LOCUS       DATCPE010000001         3866 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U7219 k141_74,
            whole genome shotgun sequence.
ACCESSION   DATCPE010000001 DATCPE010000000
VERSION     DATCPE010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955033
            Sequence Read Archive: SRR1586279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 16:49:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 640
            CDSs (total)                      :: 631
            Genes (coding)                    :: 627
            CDSs (with protein)               :: 627
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3866
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74"
                     /isolate="SMAG_U7219"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1007)
                     /locus_tag="VIM45_00005"
     CDS             complement(<1..1007)
                     /locus_tag="VIM45_00005"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY8819701.1"
                     /translation="MDFKDTPVEAIWRREVRDFLHKEMPAGLGDADGEGGLFGRGPAM
                     QEWRKRVAAKGWIAPHWPKEYGGANMSAKDQFIMNEEFAEAEAPNVGGFGVMMIGPTL
                     ISHGSEEQKRRFLPSILKGETIWCQGYSEPGSGSDLASLQTRAVRDGDDYMINGQKIW
                     TTGAQHADWMYMLARTDPDAPKHRGISMFLFDMKSPGVSVRPLTTMAGAQSFNEVFFD
                     NVRVPAPQRVGEENRGWYVGATLLDFERSGIGNAVGVRKQLERMLAYVPNESEKLAFK
                     TRSTLRLEFADRWIEASMAKWLSYRVISMQAAGLIPNHEASVTKLYMSELQQRIAALG
                     M"
     gene            complement(1086..3620)
                     /gene="lon"
                     /locus_tag="VIM45_00010"
     CDS             complement(1086..3620)
                     /gene="lon"
                     /locus_tag="VIM45_00010"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEY8819702.1"
                     /translation="MTDDPRNPDKDGDTAAETISPSQAESTGAVTGEPTDDDPAGSSA
                     ENAQSVTESAPGDEQAPAQIYIPDVLPVLRTGASVLYPAIVVPLVTSEEPDVRAIDDA
                     VTNTARIIAVVALKHGQSGAYDGDLQPVGTAAQILRMAKTPNGTLQALIQGVRRVRIV
                     GIEQDEPWIRARVESIEEVAEPSTELEALTRNAAGLFQNIVTLSEVIPGELAAAVSQI
                     NQPGNLADFIAANANLKPEDRQAVLEEANVTDRLRVLGAALAAELAVLEVSSQIRTQV
                     RGDLDKRQREFILREQMRAIQKELGDEDQPEIGELRHKIEEAHLPEEARKQADRELER
                     LAQMTQAAAEYQVARTYLEWLADLPWDRRSIDNLDIRRAEEILDEDHYGLKKVKQRIL
                     DYLAVRTLREDTRGPILCFVGPPGVGKTSLGQSIARATGRKFVRLSLGGIRDEADIRG
                     HRRTYVGAMPGRIIQEIRRAEVNNPLMILDEVDKLGSDYRGDPSSALLEALDPAQNNT
                     FVDHYLDVPFDLSHVMFITTANVLDTIPDPLRDRMEVIELDGYAEKEKLEIARRYLLP
                     RQTRENGLQPDQITVPEETLVEIISGYTREAGVRNLERLIGAICRHVARQVAAGDLEP
                     KTITPGLLPEIFGRTRYFEELANEQDEIGVVTGMAATGAGGDILFIEASAVPGKGNLT
                     LTGKLGDVMQESARAALTYARSRAAALNIAGDFFETHDIHVHVPAGATPKDGPSAGVT
                     IATAIISAITRRPVRKEVAMTGEITLRGRVLPVGAIKQKVLAAHRAGCRKICLPKENE
                     PDYADVPADVRNDLSVVFASHVDDVLNTALHDRPRAEKVRIKPTAA"
     gene            complement(3675..>3866)
                     /locus_tag="VIM45_00015"
     CDS             complement(3675..>3866)
                     /locus_tag="VIM45_00015"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEY8819703.1"
                     /translation="RAHHSGHVYNVMEIQYGPFERSILLPADVDQEQVAVEYDDGLLK
                     ITLPRRARQAHTRVRITVR"
BASE COUNT          623 a         1224 c         1214 g          805 t
ORIGIN      
        1 tgcatgccca gcgcggcgat acgctgctgc agctccgaca tatacagctt ggtaacgctc
       61 gcctcgtgat tcggaatcag gcccgccgcc tgcattgaaa tcactcgata tgagagccac
      121 ttcgccatgc tggcttcgat ccagcggtcc gcgaactcca gtcgcaatgt tgaccgcgtc
      181 ttgaaggcca gcttttcact ttcattggga acataggcaa gcatacgctc gagttgcttg
      241 cgcacaccca cggcgttgcc gatgccggac cgctcgaagt cgagcaatgt tgcgccgacg
      301 taccagccac ggttctcctc gccaacgcgc tgcggcgccg gcacacgaac gttgtcgaag
      361 aagacctcgt tgaagctctg ggcgcccgcc atcgtcgtga gtggccgtac gctcaccccg
      421 ggactcttca tgtcaaacaa aaacatggag atgccacggt gcttgggagc atcggggtcg
      481 gtgcgcgcga gcatgtacat ccagtcggcg tgctgggcgc cggtggtcca gatcttctgg
      541 ccgttgatca tgtagtcatc gccgtcgcgt acggcgcggg tctgaagcga ggcgaggtcc
      601 gatccagacc ccggctcgga atagccctgg caccaaatcg tctcaccttt gaggatgctg
      661 ggtaagaacc gacgcttctg ttcctcgctc ccgtggctga tcagggtggg gccgatcatc
      721 atcacgccga agccgcctac gttgggcgcc tcggcctcgg cgaactcttc gttcatgatg
      781 aactggtctt tagcgctcat gttcgcgccg ccgtattcct tcggccaatg gggcgctatc
      841 cagcccttgg ctgccacgcg cttgcgccat tcctgcatag ccgggccgcg gccaaacagg
      901 ccgccttcac cgtccgcgtc tccgagcccg gcgggcatct ccttgtgcag gaagtcgcgt
      961 acctcccgcc gccaaatcgc ctcaaccggg gtgtccttaa aatccatgcc gctccctact
     1021 caaccgatta cgtgcggtat cacacctacc gccgtgccag cgcagagtac catatggcgc
     1081 gggcatcacg ccgcggtcgg cttgatgcgc accttttcgg cccgtggacg gtcgtgcagc
     1141 gcggtgttga ggacgtcgtc gacgtgggac gcaaatacga ccgagaggtc gttgcgcacg
     1201 tccgctggca cgtccgcgta gtccggctcg ttctctttgg gtaagcagat ctttcgacag
     1261 ccggctcggt gcgccgccag caccttctgt ttgatggcgc ctacgggtag aacccgaccg
     1321 cggagggtga tctcccccgt catcgctact tccttgcgta ccgggcgccg cgtaatcgcc
     1381 gagatgatgg ccgtggctat cgtcacgccc gccgacgggc catctttggg agtcgcaccc
     1441 gccggaacgt ggacgtgaat gtcatgcgtc tcgaagaaat cgccggcgat gttcaacgcc
     1501 gccgcacgcg agcgcgcgta cgtcagcgcc gctcgggccg actcctgcat gacgtcgccc
     1561 agtttgccgg tcagcgtcag attgcctttg ccaggtaccg cggaggcttc gatgaagagg
     1621 atatccccgc cggcgcccgt ggccgccatg ccggttacta cgccaatctc atcctgctcg
     1681 ttggccagct cttcgaagta gcgggtacgc ccaaagatct ccggtaggag gccgggtgtg
     1741 atcgtctttg gctcgaggtc gccggccgcg acctgccggg cgacgtgccg gcaaatggcg
     1801 ccgatcagac gttcaaggtt ccgcacacca gcctcgcggg tatagccgct gataatttct
     1861 accagcgtct cctcgggaac cgtgatctgg tccggctgga gcccgttctc ccgcgtctgc
     1921 ctgggcaaca ggtagcgccg cgcgatctct agcttctctt tctcggcgta accgtcgagt
     1981 tcgatgacct ccatccgatc gcgtagcgga tcggggatcg tgtccagtac gtttgcggtg
     2041 gtaatgaaca taacgtgcga caggtcgaac ggcacatcga ggtaatgatc gacgaacgtg
     2101 ttgttctggg cgggatcgag cgcttcgagc agggccgacg atggatcgcc gcggtagtca
     2161 ctgcccagtt tatccacctc gtccaggatc atgagtggat tgttgacctc ggcacgccgg
     2221 atttcctgga tgatgcggcc gggcatggcg ccgacgtatg tgcgccggtg gccgcggatg
     2281 tctgcctcgt cgcgaattcc gcccagcgac agccggacaa actttcggcc ggtggcgcga
     2341 gcgatcgatt gtcctaggct tgtcttgcca acgccgggcg ggcccacgaa gcacagaatg
     2401 gggccgcggg tgtcttcgcg cagggtgcgc accgccaggt aatcgaggat gcgctgtttc
     2461 accttcttca gtccgtagtg gtcctcgtcc aggatctcct ctgcccgccg aatgtcgagg
     2521 ttgtcgattg agcgcctatc ccatggaaga tcggcgagcc attcgaggta ggtgcgggca
     2581 acctggtatt cggccgccgc ctgcgtcatt tgcgccagcc gctccaactc gcgatccgcc
     2641 tgcttccgcg cctcttcggg caggtgcgct tcttcgatct tgtggcgcag ctcgccgatc
     2701 tctggttgat cttcgtcgcc aagctctttc tggatcgcgc gcatctgttc ccgcaggatg
     2761 aattcccgtt gccgcttatc caggtcgccg cgcacctggg ttcggatctg gctgctgact
     2821 tccagtacgg cgagctcggc ggcgagggcg gcgccgagga cgcgcagtcg atcggtaacg
     2881 ttggcctctt cgagaaccgc ctgccggtcc tccggcttga ggttggcgtt cgccgcgatg
     2941 aagtcggcca ggttgccggg ctgattgatc tgtgagactg ccgcggcgag ctcgcccggg
     3001 atcacctcgc tcagggtaac gatgttctgg aaaaggccgg ccgcgttgcg agtcagcgct
     3061 tcgagctccg tgctcggttc agctacctcc tcgatgcttt cgacccgggc gcgaatccac
     3121 ggctcgtctt gctcaattcc gacgatgcga acgcgcctga cgccctgaat aagcgcctga
     3181 agcgttccgt tcggcgtttt ggccatacgg aggatctgcg cagcggtccc gaccggctgc
     3241 aggtcgccat cataagcgcc gctctggcca tgcttcaggg cgaccacggc gatgattcgg
     3301 gcggtgttcg tcacggcgtc gtcgatcgcg cggacgtcgg gctcttcgga cgtcacgagc
     3361 ggtacaacga tcgccgggta cagcacactg gcgccggttc gaagaacggg caatacgtcc
     3421 gggatgtaga tctgtgcggg tgcttgctcg tcgccgggcg cgctttccgt gacggattgt
     3481 gcgttctcgg ctgaagagcc cgcgggatcg tcgtccgtcg gttcgccggt taccgcaccg
     3541 gtcgattcag cctgggaggg cgaaattgtt tcggcggcag tatccccgtc cttgtcggga
     3601 tttcgaggat catccgtcat gcttatagcc tcgcgggagg gttggaattt gaagaacggg
     3661 cggagtagac cgcgtcagcg aacggtgatc cgaacccgag tgtgcgcctg tcgggcgcgg
     3721 cgtgggagcg taatctttag cagcccgtcg tcgtactcca cggcgacctg ttcctgatcg
     3781 acgtccgcgg gcagcagaat cgagcgctcg aaaggcccat attggatttc catcacgttg
     3841 tacacgtgac ccgaatgatg cgcccg
//
LOCUS       DATCPE010000002         5665 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U7219
            k141_194499, whole genome shotgun sequence.
ACCESSION   DATCPE010000002 DATCPE010000000
VERSION     DATCPE010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955033
            Sequence Read Archive: SRR1586279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 5665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 16:49:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 640
            CDSs (total)                      :: 631
            Genes (coding)                    :: 627
            CDSs (with protein)               :: 627
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5665
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194499"
                     /isolate="SMAG_U7219"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..558
                     /locus_tag="VIM45_00020"
     CDS             <1..558
                     /locus_tag="VIM45_00020"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012347.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEY8819704.1"
                     /translation="SNATQVKEEDVKAGTAKPVSEDQASRMGTLHPYLRSVNTDANSP
                     LAIAVWSFIFVEFWGITALGARHYFGKFLAFPPFAKSASPINIFVGLLETLSEFIRII
                     SFTFRLFGNIFAGDVLILFMSFLAPFILPVVFYGLETFVGFIQAAVFALLTLVFAVMA
                     VESHDEGEHDAHDAHGERAEAAAHG"
     gene            659..904
                     /gene="atpE"
                     /locus_tag="VIM45_00025"
     CDS             659..904
                     /gene="atpE"
                     /locus_tag="VIM45_00025"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HEY8819705.1"
                     /translation="MLLAADITDKGIANLGAGLAIGLGAIGPGLGIGMLVGRAMEALG
                     RNPEARPAIQTNMILGVAFAEAIGIYALLVAIIIKFV"
     gene            999..1505
                     /gene="atpF"
                     /locus_tag="VIM45_00030"
     CDS             999..1505
                     /gene="atpF"
                     /locus_tag="VIM45_00030"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEY8819706.1"
                     /translation="MQAFDALGINLPSLIAFIVNFGLLMLLLRLTLYRPVLKMLDERR
                     HRIQESLGAADRAAESARESEQRVAEEIRQAQLQGQEIVGSAQQIAQRIQDDAAREAQ
                     VRAQQILDQAQASILQETEAARAALRREFADLTILAAEKVINQSLDKQSHLRLINEVL
                     AESGSGRG"
     gene            1560..2096
                     /locus_tag="VIM45_00035"
     CDS             1560..2096
                     /locus_tag="VIM45_00035"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004402.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEY8819707.1"
                     /translation="MRREIAARRYAEAVFQIARDQGTIDQWRAEVQTVGSVFADPEVL
                     GLLENANVPHQAREDVLNRTLEGISPLALNFARLLVQRRRVALAPQVAEVFGELADAY
                     VGVAHAEVTTAVDVDDSERRLIAERLSQLTGKHVDVHTRTDPTILGGMVARIGDRLID
                     GSVRTRLQMLRRRLETAR"
     gene            2109..3629
                     /gene="atpA"
                     /locus_tag="VIM45_00040"
     CDS             2109..3629
                     /gene="atpA"
                     /locus_tag="VIM45_00040"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEY8819708.1"
                     /translation="MAVRPDEISSIIKNEILTFGSQVQQTDVGTVIEAGDGVVRIHGM
                     SGALYGELIEFPYPDKTITGIALNLEEDTVSGVVLGDYTKIKEGDEARSTGRVAEVPV
                     GDALIGRVVNSLGEPIDGKGPINTTKFRAVERIAPNVVTRQGVNQPVQTGIKAIDAMF
                     AIGRGQRELIIGDRQTGKSAIALDTIINQKGGDLICIYVAIGQKVSKVAQTVAALEKA
                     GAMDYTIVVAANASDSAALQYLAPYSGCAMGEEFMEQGKDALIVYDDLSKHAWAYRQM
                     SLLLRRPPGREAYPGDVFYLHSRLLERAAKLSAEFGGGSLTALPIVETQANDVSAYIP
                     TNVISITDGQIYLESDLFNAGQRPAINIGLSVSRVGSAAQTKAIKQVAGRLRGDLAQY
                     GELQAFAQFGTSDLDALTRRQLERGQRAVEVLKQPQYAPLNLVEEVVSLFALTNGYLD
                     DLPVEKIIPFEQSLRKFVQSSHPEIAEAINNEKALSDATTDALKRAIDESKATVPY"
     gene            3827..4714
                     /gene="atpG"
                     /locus_tag="VIM45_00045"
     CDS             3827..4714
                     /gene="atpG"
                     /locus_tag="VIM45_00045"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HEY8819709.1"
                     /translation="MPSYRQIKRRIRSVTNTAKITNALQLVAASKMRRAQMRASAARP
                     YSERMRSVLAKLSQQVQASGLDEESVHPLLRQRTGNRVTLVVVSPQRGLSGGLPGNLN
                     RRVFQLIRELGPEVRIEAIAVGKKGRDFLVRGGYRLAAEYLDLGDYPAAPDITPISRE
                     VIDAYLQGTTDRVMMVYSDFINTVTHRPTVRQLLPVEPSETAERTGGTTIDYIYEPSA
                     AAVLAQLLPRYIEMQIYEAVLQNVASQYSAQMVAMRNATDAANDMVEALTLVANKARQ
                     EQITKELLDIVGGVAAVGG"
     gene            4739..5398
                     /locus_tag="VIM45_00050"
     CDS             4739..5398
                     /locus_tag="VIM45_00050"
                     /inference="COORDINATES: protein motif:HMM:TIGR01509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEY8819710.1"
                     /translation="MPQRIDAVVFDMDGVLLDSEPLHFITVNELLAEEGGVLELAEYL
                     GYVGTTLEDTWADLVRRFRLKRSFEYYRDRYDAEILRHYRDSAVLLPGAAWLMEELLR
                     RGTRLAVASSSRTEWVEVCLAKLGLLNKFHAIVTGDMVTQGKPDPEIYLKASAALRVA
                     PERCLAIEDAPKGVQSAHRAGMTVVAVDTQYTAALDIPEADARIPTLDHFDLHWLDAA
                     A"
     gene            5471..>5665
                     /locus_tag="VIM45_00055"
     CDS             5471..>5665
                     /locus_tag="VIM45_00055"
                     /inference="COORDINATES: protein motif:HMM:NF014873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8819711.1"
                     /translation="MAEGRVAQIIGTVVDLEFPPDQLPEIFNAVEIYLDPHGAKEHEL
                     EIGEGNPNGTSNIVVGEVQQH"
BASE COUNT         1161 a         1641 c         1836 g         1027 t
ORIGIN      
        1 tccaacgcca cacaggtgaa agaagaggat gtgaaggccg gaacggcgaa accggtcagc
       61 gaagaccagg cgtcgagaat ggggacccta catccctatt tgcgcagcgt gaacacggac
      121 gcgaacagcc cgctggcgat cgccgtctgg tcgtttatat ttgtcgagtt ctggggtatc
      181 acggcactcg gcgctcgtca ttactttggc aagttcctgg ccttcccgcc gttcgcaaag
      241 agcgccagcc cgatcaacat cttcgtcgga ttgctggaaa cgctcagcga gttcatccgg
      301 attatcagtt tcaccttccg tctcttcggc aatatcttcg ctggcgacgt gttgatcctg
      361 ttcatgtcgt tcctggcgcc gttcatcctg ccggtggtgt tttacgggct ggagacgttc
      421 gtaggtttta ttcaagcggc agtctttgcg ctgctcaccc tcgtttttgc cgtgatggcc
      481 gtagagtcgc acgacgaggg cgagcacgat gcgcatgatg cacacgggga gcgtgcggaa
      541 gcggcggcgc acggctagta tccccgagcg tgagcgcacc gcgcgtgagc gtgcgcgatt
      601 acaaaagccc aaccagggct cggaaaagga cgaggaagag agaatgacgt ttctgatgat
      661 gttgctcgcg gcagatatta cggataaggg tattgcgaac ctcggcgccg gtctcgcgat
      721 cggccttggc gcgatcgggc ccgggctcgg catcggcatg ctggtaggaa gggcgatgga
      781 ggcgctgggc cgcaaccctg aggcccggcc ggcgatccag actaatatga tcctcggcgt
      841 tgcttttgcg gaagcgatcg ggatctacgc cttgctcgtc gcgattatca tcaagttcgt
      901 ttaggcaggc acattgttga aacggcgagt gccgggccgg cggcgttagc cggcgccccg
      961 agcccactgc gcggcgtccg gacgaatcgg aggtacaggt gcaggcattt gatgccctgg
     1021 gaatcaacct tcccagcctg attgcattca tcgtcaattt tggtctgctg atgctgcttc
     1081 tgcggctgac gctctatagg ccggtgctaa agatgctgga cgagcgtcgc caccggattc
     1141 aggagagcct gggagcggcg gaccgagcgg ccgaatcagc ccgcgagtcc gagcagcgcg
     1201 ttgcggaaga gatccgtcag gcgcagttgc agggacagga aatcgtcggc agcgcccaac
     1261 agatcgcgca gcgcattcag gacgacgcgg cccgagaagc gcaggtacgg gcgcagcaga
     1321 tcctcgacca ggcccaggca tccattctgc aggaaactga agcggcccgc gcggcgcttc
     1381 ggcgcgagtt cgcggatttg acgattcttg ccgcggaaaa agtaatcaat cagtcattgg
     1441 acaagcagtc gcatctgcgg ttgatcaacg aagtgctggc ggagtcgggc agtgggcgcg
     1501 gttagagccg gcggccgcgc cgtgcgccgg cctaaacgcc aagaacggaa tgcgcgggta
     1561 tgagacgcga aatcgcggcc agacgatacg ctgaagcggt gttccagatc gcccgcgatc
     1621 agggcacgat tgaccagtgg cgggcagaag ttcaaacggt cggctccgtc tttgcagatc
     1681 ccgaggtcct tggcctgctc gagaacgcca acgtgccgca tcaggcgaga gaggacgtgc
     1741 tgaaccgcac gctggagggc atatcgccgc tggcgctgaa ttttgcgagg cttctcgttc
     1801 agaggcggcg cgtcgcgctg gccccgcaag tggcagaagt cttcggcgag ttggccgacg
     1861 cgtacgtagg agtcgctcac gccgaggtta cgaccgccgt tgatgtagat gacagtgaga
     1921 gacggcttat tgctgaacgc ctcagccagt tgacgggcaa gcacgtagac gttcacacgc
     1981 gtacggatcc caccatcctc ggtggaatgg tggcgcgcat cggcgatcgg ctgatcgatg
     2041 gcagcgtccg gacacggcta cagatgctgc ggcgccggct cgagacggca cggtaaggag
     2101 atattcgcat ggcagttcgc ccggatgaaa tttcttcaat aatcaagaat gaaattctaa
     2161 cgtttggatc gcaggtccag cagacggatg tcggcacggt tatcgaggct ggcgacggcg
     2221 tggtgcgcat ccacggcatg agcggcgccc tctacggcga gctaattgag ttcccgtacc
     2281 cggataagac gattaccggt attgcgctga accttgaaga agatacggtc agcggtgtag
     2341 tgctgggcga ctacaccaaa atcaaagagg gcgacgaggc gcgatccacg ggtcgcgtcg
     2401 ccgaggttcc cgtcggagat gcgttgatcg gccgcgttgt gaactcgctt ggcgagccca
     2461 tcgacggcaa gggcccgatc aacaccacca agttccgggc cgtcgaacgc atcgccccca
     2521 acgtggttac acgccagggc gtaaaccagc ccgtgcaaac cggcatcaag gcgatcgacg
     2581 ccatgttcgc gatcggccgc gggcaacgtg agttgatcat tggcgaccgg cagaccggga
     2641 aatccgccat cgcgctcgac acgataatca accagaaggg cggcgacctg atctgcatct
     2701 atgtcgccat cggccagaag gtctcgaagg tcgcgcagac cgtcgcggcg ctcgaaaagg
     2761 ccggcgccat ggattacacc atcgtggttg ccgccaatgc ctccgactcg gccgcgttac
     2821 agtaccttgc cccgtattcc ggttgcgcca tgggtgagga gttcatggag cagggcaagg
     2881 acgccctgat cgtatatgac gacctcagca agcacgcatg ggcgtaccgc caaatgtcgt
     2941 tgctgctgcg ccggcctccc gggcgcgagg cgtacccggg cgacgttttc tacctgcaca
     3001 gccggttgct ggaacgagct gccaagctgt cggccgagtt tggcggtggg tcgctcaccg
     3061 cgctgccgat cgtcgagacg caggcaaacg acgtgtcggc atacattccg accaacgtca
     3121 tctctatcac cgacggccag atctacctgg aatccgacct gttcaacgcc ggccagcgac
     3181 cggcgatcaa catcggactt tcggtttcgc gcgtcggtag cgccgcacag acgaaggcga
     3241 ttaagcaggt ggccggccga ctcaggggcg acctcgccca atacggagaa cttcaggcct
     3301 tcgcgcaatt tggcacgagc gatctggacg cgctcacgcg acgccagctc gagcgcggac
     3361 agcgcgccgt cgaggtactg aaacagcccc aatacgcgcc gttgaacctg gtcgaagaag
     3421 tcgtcagcct gttcgccctc acgaacggct atctcgacga tctgccggta gagaagatca
     3481 ttcccttcga acagtcgctg cgaaagttcg tccagagcag ccaccccgag attgcggagg
     3541 cgatcaacaa cgaaaaggcg ctgagcgacg cgacgactga cgcgctcaag cgggcgatcg
     3601 acgagtccaa agcgacggtt ccgtattagg ccgcggacct cacccccttc ccctctccat
     3661 cgttcacgat ggagaggggc gatctagggt tgctggattc ggacttggtg gcagcgtcgg
     3721 gcggggcgca gggcgatgcg cccccacgaa tgggcggaat gccccacaaa atatcgagtt
     3781 gaagctcccc ttcccttatg gggaaggggc cgggggttag gtcgagatgc cgagctaccg
     3841 tcagatcaag cgacgtattc gctcggtcac gaatacggcc aagattacca acgcgttgca
     3901 gctagttgcg gcatcgaaga tgcggcgcgc ccagatgcgg gcgtcggcag cgcggccgta
     3961 ttcggagcgc atgcgctctg tactcgccaa gctgtcgcag caggtacaag cctctggctt
     4021 ggacgaggag tccgtgcacc ctctcttgcg acagcgaacg ggcaatcgcg tgacgttggt
     4081 cgtagtctcg ccacagcgag ggctatcggg cggactcccg ggtaacctga accgtcgcgt
     4141 ctttcaactg atccgcgaac tcgggcccga ggtacgcatc gaggcgatcg cggtcggcaa
     4201 aaaggggcgt gattttctcg tgcgcggcgg gtaccggctg gcggccgaat atctggatct
     4261 aggggactat ccggccgcac ccgacataac gccgatctcg cgagaagtca tcgacgccta
     4321 cctccagggc actaccgatc gcgtcatgat ggtctattcc gacttcatca acactgtgac
     4381 ccaccgcccc accgtacgac agttactgcc cgtcgaaccg tccgagaccg ccgagcggac
     4441 ggggggaaca acgatcgact atatttacga gccgtcggcg gcggcggttc tcgcccagtt
     4501 gctgcctcgc tacatcgaga tgcagatcta cgaggcagtg ctgcagaacg tggcgagcca
     4561 gtactcggcg cagatggtgg cgatgcgtaa cgcgaccgac gcggcgaacg atatggtgga
     4621 agcgctcacg ctcgtagcga acaaggcgcg tcaggagcag attacgaagg aattgctcga
     4681 tatagtaggc ggcgtcgccg cggttggtgg gtagtcagac atcaggagac cacataccat
     4741 gccccagcgg attgatgcgg tggtgttcga tatggacggg gttttgctgg acagcgaacc
     4801 gttgcatttt attacggtga acgagctatt ggctgaagaa ggcggggtcc tggaattggc
     4861 cgagtacttg ggctacgtgg ggacgacgct cgaagacacc tgggccgatc tggtgagacg
     4921 cttccgactc aagcgatcgt ttgagtacta tcgcgaccgg tacgacgccg aaatcctgcg
     4981 ccattaccgc gacagtgccg tgctcctgcc cggtgcagcc tggttgatgg aggaactgtt
     5041 acggcgcgga acgcgattgg ccgtggcttc gtcgtcccgc accgagtggg tggaagtttg
     5101 cctggcgaag ctgggtctgc tcaacaagtt ccacgcgatc gtgaccggag acatggtgac
     5161 ccagggcaag ccggatcccg agatctattt gaaggcctcg gccgcgctca gggtcgcgcc
     5221 ggaacgctgc ctggcgatcg aagacgcgcc caagggcgta cagtcggccc accgagctgg
     5281 aatgacggtg gtagcggttg atacccagta cacggcggcg ctggatatcc ctgaggcgga
     5341 tgccaggata ccgacgctgg accatttcga tttgcactgg ttggatgccg cggcctgaat
     5401 ccggtcgcgg accagcggag ccgggtggca aacgcagcag ggccgtggtt agggaaggaa
     5461 cttaaacgta atggcagaag gacgagtagc gcagattatc ggcaccgttg tcgacctgga
     5521 attcccaccg gaccaactgc ccgagatctt caacgcggtc gaaatctacc tcgatcccca
     5581 cggtgccaaa gagcacgaat tggagatcgg cgaagggaat ccgaacggaa cgagcaacat
     5641 cgtcgtcggg gaggtgcaac agcat
//
LOCUS       DATCPE010000003         1922 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U7219
            k141_453791, whole genome shotgun sequence.
ACCESSION   DATCPE010000003 DATCPE010000000
VERSION     DATCPE010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955033
            Sequence Read Archive: SRR1586279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 16:49:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 640
            CDSs (total)                      :: 631
            Genes (coding)                    :: 627
            CDSs (with protein)               :: 627
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1922
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_453791"
                     /isolate="SMAG_U7219"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            34..438
                     /locus_tag="VIM45_00060"
     CDS             34..438
                     /locus_tag="VIM45_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8819712.1"
                     /translation="MSESDIDPLARKAQADLIVDRAAGQLRRIVHQLAEQLEPFPPFP
                     GAFFTNAIEVEPDAAGSADRGCVVVGPDGDLYELIMGVEFDSPEAFVDPVAARTEELR
                     KLELHPRDYVVYAYNAVGALAEALLQKAEEGL"
     gene            complement(452..1411)
                     /locus_tag="VIM45_00065"
     CDS             complement(452..1411)
                     /locus_tag="VIM45_00065"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEY8819713.1"
                     /translation="MSLPSPERRLWRRRQFLAAPAGFVGGVVVARRIEGVSRALASMA
                     AVSLSPGAPLAFLSTNTVAQGEAFSLHVQTFFVSSGTAAFNDVQFPLITAGEYLVGML
                     AAGQSVTDEVEIDPGDYNVVVDAVDSNGAPLTFTLPITVSNTPFPVDEIQLDAAIASL
                     LSPATVQQELTKLEQIYGTAKGPRLWKGLFRVPVTGPITTEFGQARSYNGGPISGHHS
                     GIDIAANLGTPVGAAASGRVSFTGALPERGNFITIDHGMGVYTGYAHLSQINTAVGVS
                     VSPGDIIGLVGSTGLSTGPHLHWECAVNGVHVSAMPWTRTLLP"
     gene            1677..>1922
                     /locus_tag="VIM45_00070"
     CDS             1677..>1922
                     /locus_tag="VIM45_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEY8819714.1"
                     /translation="MEDPRSIAERIISNVEKVIVGKHEELRLTLTALLCQGHVLIEDV
                     PGVGKTMMARSIARSTGCVFKRIQFTPDLLPTDVTGVS"
BASE COUNT          376 a          581 c          612 g          353 t
ORIGIN      
        1 ctatactcgg agcgcaggag gcgccaatcg aggatgtctg aatccgatat cgatccgctc
       61 gctcgcaagg cgcaggccga cctcatcgtc gatcgcgccg ccggtcagct acgacgcatc
      121 gtccaccagc ttgccgagca actggaacca ttcccgccct tccccggtgc gttcttcacc
      181 aatgcgattg aagtcgagcc cgatgccgcc ggcagcgcgg atcgcggctg tgtcgtcgtc
      241 ggcccggacg gcgatctcta cgagctgatc atgggcgtgg agttcgacag cccggaagcg
      301 tttgtcgatc ccgtcgccgc ccgaacggaa gagctacgca agctcgagtt acacccgcgt
      361 gactacgtcg tttacgccta taacgcggtt ggagccctgg ccgaggcgct cttgcagaag
      421 gcggaagagg ggttgtaaga cgtggcacgg tctacggcag cagcgttcgc gtccaaggca
      481 tggcgctgac gtgcacaccg ttgacggcgc attcccaatg taggtgaggc cccgtagata
      541 gccccgtgct gccgaccagg ccgatgatgt cccccggcga aaccgatacc ccgacggcgg
      601 tgttgatctg ggacaagtgt gcgtatcccg tgtagacacc catgccgtgg tcgatcgtga
      661 tgaaattgcc cctctctggc aaagcgccgg taaacgaaac gcggccgctg gcggctgcac
      721 ccaccggcgt ccccagattg gccgcgatat ctatccccga atgatgaccg ctgatcggac
      781 cgccgttata cgagcgcgct tggccgaact cggtcgtgat cgggcccgta actggcacgc
      841 ggaagagtcc cttccagagc cgaggtccct ttgcggtgcc ataaatctgc tcgagcttgg
      901 tcaactcttg ctggaccgtg gcggggctga gcaatgaggc aatggccgca tcgagctgaa
      961 tctcgtctac ggggaacggt gtatttgaaa cggtgatcgg cagcgtgaag gtgagcggag
     1021 cgccgtttga gtcgacggcg tccacgacga cgttgtaatc gccgggatcg atttccacct
     1081 cgtctgtcac cgattgacct gcagccagca tgccgacgag atactcaccg gcagttataa
     1141 gcgggaactg cacgtcattg aaggctgcag taccggagct tacgaaaaac gtctgcacat
     1201 ggagcgaaaa tgcttcgccc tgcgccacgg tattggtgga gagaaacgcg agcggagcac
     1261 cgggcgatag ggagactgcc gccatgctgg cgagcgctcg cgagacgccc tcgatccgac
     1321 gggctacaac cactccgccg acgaatccgg cgggagccgc caggaattgc cggcgtcgcc
     1381 acaagcggcg ctccggactt ggcagcgaca cgtaccatct cgcaaggcaa aggcgcctta
     1441 tgcagtgtat acggaggagg gtaaacgcgg cgtactcgcc ggtccgcgcg gctattcgaa
     1501 tcgcgcggac gcctctgcta tacttccagt agaaaaacgg cggaaaattc caacagaggc
     1561 tgtgcgctcc cgtgccggcc tcgtttggtc ataccggtcg gcgttgcccg tcttcgtggt
     1621 gatagtgcgg cgacgcgagt tcggccggcg ttcagggacc gtggaggagt gtggcggtgg
     1681 aagacccgcg atcgatcgcc gagcggatca ttagtaatgt cgagaaggtc atcgttggga
     1741 agcacgagga gcttcgcctg acgctaacag cgctgctttg ccagggccac gtgctcatcg
     1801 aggacgtccc gggcgtgggt aaaaccatga tggcccgcag tattgcgcgt tctaccggtt
     1861 gcgtcttcaa acgcatccaa tttacaccgg acctgctccc gaccgacgtg accggggttt
     1921 cg
//
LOCUS       DATCPE010000004         2678 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U7219
            k141_291806, whole genome shotgun sequence.
ACCESSION   DATCPE010000004 DATCPE010000000
VERSION     DATCPE010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955033
            Sequence Read Archive: SRR1586279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 16:49:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 640
            CDSs (total)                      :: 631
            Genes (coding)                    :: 627
            CDSs (with protein)               :: 627
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2678
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291806"
                     /isolate="SMAG_U7219"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..795
                     /locus_tag="VIM45_00075"
     CDS             <1..795
                     /locus_tag="VIM45_00075"
                     /inference="COORDINATES: protein motif:HMM:TIGR01826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconeogenesis factor YvcK family protein"
                     /protein_id="HEY8819715.1"
                     /translation="ALADTEPLIRSLFEYRFPEGTDLEGHSFGNLFIVAMSEVTGSFE
                     EAIRATSRVLVTRGQILPSTLADVTLTALTEDGERVRGESTIGHIGKRIREVFIEPPD
                     ALPHPEAVGAILEADLIIFGPGSLYTSVLPNLLVRGISESLRASNALKIYVSNVATQH
                     GETDSFTVSDHVKALESHVGKGMFDLVIANSNVSNELPEEWHSEPVRVPSEAATDPRI
                     VEVDVISEENRYHHDSVKLAAAIMRLYHGRGQSVVDYRPPALTGTG"
     gene            834..1073
                     /gene="secG"
                     /locus_tag="VIM45_00080"
     CDS             834..1073
                     /gene="secG"
                     /locus_tag="VIM45_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEY8819716.1"
                     /translation="MLLAINISTTLNLAQLIVSIVLSIVLLLQAKGSGFGAGLGGQSS
                     VFRTRRGIEKTLFQLTIVLIFMFVFVSILSVRQGA"
     gene            1123..>2678
                     /locus_tag="VIM45_00085"
     CDS             1123..>2678
                     /locus_tag="VIM45_00085"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY8819717.1"
                     /translation="MRLLFNRFCSPGAWAFILLVAGVPLLALNWAVASNSGGGSLPAA
                     GGRYVEGDIGPAPERINPLFAANTGPEADLAGLIFSGLTRTGPNGESQPDLAEDWVAS
                     IDGTSFTFDLRQDVLWQDGQPFTADDVLFTARTFAVPGVKGDPATAEVWRRARVDKIG
                     LYTVVFQFSSSFAPFLAYSSAGILPQHLLGKDSPEQLVSDQFNRHPVGTGPYRLQSLT
                     ASGAELVRYTAYHLGTPYVDHISMRFLKDNQTLIGALRSHQVDGGILPPPLASSQLDS
                     LRGTGHRLISGGRPAYSLVYLNLNIAQFQDVAVRQALSLATDRNGLVQQVMDGQATAA
                     DVPLPPGTWTSAGSPPNGGQSAARQALQKAGWALDANGVQQRQGISLSFTLQTTDDPQ
                     RRATADALARGWAAIGVKANVQTVDEATLLSNILLPHKYEAVLYGWDPGPDPDPFPAW
                     HSSQKEQQGSNLSGYSSARADQLLEAARQTADPVDRAQIYGSFADVFRQDVPAVVLFF
                     PRFVYVLPQRL"
BASE COUNT          505 a          864 c          790 g          519 t
ORIGIN      
        1 gctctggccg acacggagcc gctgatccgc agcctctttg agtaccggtt tccagaaggc
       61 acggaccttg aagggcacag cttcggcaac ctcttcatcg ttgcgatgag cgaggttacc
      121 ggcagcttcg aggaagcaat acgcgccacc agccgggtgc tcgtgacacg cggccagatc
      181 ctgccgtcaa ccctcgcaga cgtgaccctc accgcgctca ccgaggacgg cgaacgcgtt
      241 cgcggtgagt cgaccatcgg ccatattggc aagcgcatcc gcgaagtgtt catcgagccg
      301 cctgacgcgt tgccccaccc cgaagccgtc ggcgccatac tcgaagccga tttgatcatt
      361 tttggacccg ggagcctcta taccagcgtg cttcccaacc tgctcgtgcg cggcatttcc
      421 gaatcgcttc gtgcctccaa cgcgctcaaa atctacgtga gcaacgtagc gacgcaacac
      481 ggcgaaaccg acagttttac tgtttccgac cacgtgaaag ctctggaaag ccatgtcggt
      541 aaagggatgt ttgacctggt gatcgccaac agcaacgtgt cgaacgaatt gccagaggag
      601 tggcactccg agccggtccg cgtcccatct gaggcggcga ctgatccgcg aatcgtcgag
      661 gtagacgtga tcagcgagga gaaccggtac caccacgact cggtgaaact tgctgcggcc
      721 atcatgcgct tgtaccatgg tcggggccag tcggttgtcg actaccgacc tcccgctctg
      781 acggggaccg gctgaggctc ggctatttca ctcttcgaga agaccaacta cgtatgcttc
      841 ttgcgattaa cattagtacg accctgaacc tcgcccaatt gatcgtatcg atcgtcctat
      901 cgatcgtcct gctgctgcag gcgaagggat ccggtttcgg tgccgggctc ggcggccaga
      961 gctcggtatt tcgcacccgc cgaggcatcg aaaagacgct cttccagttg acgatcgttt
     1021 tgatcttcat gttcgtcttc gtttcgatcc tcagcgtgcg ccagggcgct tagccggcgg
     1081 gcactgctac gccttcctca gcgctccagg cgggacggac gaatgagact cctctttaac
     1141 cggttctgtt ctccgggcgc gtgggcgttc atcttgctcg tcgcgggtgt gccgttgctt
     1201 gcgctgaact gggcagtcgc ttcaaattcc ggcggcggct ctctgccggc agcgggcggc
     1261 cgctacgtag aaggcgatat tggacccgcg ccggagcgca tcaatccgct atttgccgca
     1321 aacaccggac cggaagcaga cctggcaggg ctgatcttct ccgggctcac gcgcaccggt
     1381 ccaaacggcg agtcacagcc cgatctggct gaagattggg tcgcgtcaat cgacggcacc
     1441 agcttcacct tcgaccttcg tcaggacgtg ctctggcagg acgggcagcc gttcaccgct
     1501 gacgacgtgc tgttcacggc gaggaccttt gcggttccgg gagtcaaagg cgaccctgcc
     1561 acggccgagg tttggcgcag ggcgcgggtg gacaagatcg gtctctacac cgtcgtcttc
     1621 cagttctcgt cgtcgttcgc accgtttctc gcctatagct ccgccggcat cctgccgcag
     1681 cacttactcg gcaaggactc ccccgagcag ttggtcagcg accaattcaa ccgccatcca
     1741 gttgggaccg gaccatatcg actccaatcg ctgacagcct ccggcgctga actggtgcgg
     1801 tacaccgctt accatctggg aacgccgtat gtcgaccaca tctcgatgcg ctttctcaag
     1861 gacaaccaga cccttatcgg ggcgctccga tcgcaccagg tagacggagg cattttgccg
     1921 ccgccgctag cttcgagcca actggactcc ctgcggggca ccggacacag gctaatttct
     1981 ggcgggcggc cggcgtacag tctcgtctac ttgaacctga acatcgcgca gttccaggac
     2041 gtcgccgtgc gacaggcgct cagcctggcg actgatcgaa atggcctggt gcaacaggtc
     2101 atggacggcc aggcgactgc cgccgacgtt ccgctgccgc caggcacgtg gacgagcgcc
     2161 ggttcgccgc cgaacggcgg gcaatccgcg gcccggcagg cgctgcaaaa ggcgggctgg
     2221 gccctggatg ctaatggagt gcaacaacgc cagggaattt cacttagctt tacgctccaa
     2281 acaaccgatg acccgcaacg acgcgcgacg gccgacgcac tggcacgcgg gtgggcggcc
     2341 atcggcgtca aggcaaacgt tcaaacggtg gacgaggcga cgttgctttc caacatattg
     2401 ctgccgcaca aatacgaagc ggttttgtac ggctgggatc ccggccccga tccggaccca
     2461 tttccagcct ggcatagttc tcagaaggag caacagggca gcaacctcag cggatactcg
     2521 agtgcgcggg ccgatcagct actcgaagct gcccgccaaa ccgcggaccc ggtcgacagg
     2581 gcccaaatct atgggagctt tgccgatgtc ttccgccagg acgttccagc ggtcgtgctg
     2641 ttcttcccgc gcttcgtcta tgtgctcccg caacgtct
//
LOCUS       DATCPE010000005        11929 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U7219
            k141_615924, whole genome shotgun sequence.
ACCESSION   DATCPE010000005 DATCPE010000000
VERSION     DATCPE010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955033
            Sequence Read Archive: SRR1586279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 11929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 16:49:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 640
            CDSs (total)                      :: 631
            Genes (coding)                    :: 627
            CDSs (with protein)               :: 627
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11929
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_615924"
                     /isolate="SMAG_U7219"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="VIM45_00090"
     CDS             complement(<1..238)
                     /locus_tag="VIM45_00090"
                     /inference="COORDINATES: protein motif:HMM:NF013691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEY8819718.1"
                     /translation="MVVSKSSPRVECPGRKTECYGDFMTTDREATALAAHRWQLYLLR
                     CRDGTLYTGISTDVVRRVQEHDTGRGARYTRGRSP"
     gene            403..618
                     /locus_tag="VIM45_00095"
     CDS             403..618
                     /locus_tag="VIM45_00095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8819719.1"
                     /translation="MSTGEAMFLGFLSGVIIFFVEHFWVHASWGLSSIIGVGVAVAST
                     ALNVWLESRRRSKFAAEVKEARRSRRR"
     gene            675..860
                     /locus_tag="VIM45_00100"
     CDS             675..860
                     /locus_tag="VIM45_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8819720.1"
                     /translation="MTNVEELAQSTDLASMRNELAALLQERCFSYGYQYQALYQADSS
                     GKLQAASGIAGAAGRID"
     gene            complement(867..1307)
                     /locus_tag="VIM45_00105"
     CDS             complement(867..1307)
                     /locus_tag="VIM45_00105"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8819721.1"
                     /translation="MTGEEPLFEDASMSVDTGPQTNAGLVMIIDDDPQICDLISVALS
                     EQGYRTVTAGDGMQALPVIERDPPSLILMDVGMPALGGRDLAARVNELWGDAIPCVIM
                     SGSRPSAEDRADKAVAAYLPKPFELEELFGVVRHHARTVQITHR"
     gene            1595..5395
                     /locus_tag="VIM45_00110"
     CDS             1595..5395
                     /locus_tag="VIM45_00110"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014435082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0032549 - ribonucleoside binding [Evidence
                     IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEY8819722.1"
                     /translation="MTSARDLAPVRAAVPSVRKVYSKIREIISVPNLIQVQLDSFLWF
                     TGAKDAASKAESGLRELFDEISPIVDFTGSRMELRFLDYEFGEPKYSELECRDKDMTY
                     SAPLRVDVELRVKETEEIKEQQLFMGDFPLMTKNGTFIINGAERVVVSQLVRSPGVYF
                     TVEQDPSSGRDMTGAKLIPNRGAWLEFETSNKDIVSVKVDRKRKIPITTLLRAIDRDP
                     AIDDETKLGTDERILTKFEDVESLSEIHHYIRTTLERDSARTKRQALEEFYKRLRPGD
                     PPTLENARQLLDSLFFNPRRYDLGRVGRYKLNKRLGVSVDQKTRTLTHDDIVSIVKQL
                     IRINLGQGLPDDIDHLGNRRVRAVGELIQNQFRIGLLRMERVVRERMTITDPDQATPS
                     ALINIRPVVAAMKEFFGGSQLSQFMDQTNPLAELTHKRRLSALGPGGLSRDRAGFDVR
                     DVHHSHYGRICPIETPEGPNIGLIGSLATFGKVNPYGFIETPYRKVLRELPKDSVDLV
                     GHTLREDLRDGKGVIAAAGTEVSRALAKKIASNGVERVKVRPFVSDIVEYLSADVEDN
                     YVVAQANVALDDQGQFLETHVEVRHRQDFRVELADHVDYMDVSPKQIVSVATSLIPFL
                     EHDDANRALMGSNMQRQAVPLLRPEVPLVGTGMEKQTAFDSGQVVFAHKDGRVVSSTA
                     KRIIVEYVDGEREEFRLQKFVRSNQGTCINQRPIVERGQQVIAGQALADSSSTDQGEL
                     ALGQSVLCAFMSWEGYNFEDAIIISEAICREDKFTSIHIEKHEIEARDTKLGPEEITR
                     DIPNVGEDSLRDLDEDGVIRVGAEVREGDILVGKITPKGETELTAEEKLLRAIFGEKA
                     REVKDTSLRVPHGERGKVIDVKVFDRESHLELPAGVNKLVRVSIAQKRKLSEGDKMAG
                     RHGNKGVVARILPVEDMPYLADGRPVDIVLNPIGVPSRMNLGQVLETHLGWAAKTLGF
                     RAITPVFDGASDVKIEDALARAWLTMEAGAVSLNPISGQTTTKTEDEVGEYIDLERLR
                     EWLGERGYNPDEVFDDSHPGAARRACLELWMEQVGEKKVRGRSLEELDELADRMSADR
                     RMAPPIFGKQVLYDGRSGEAFDQPVTIGYIYMLKLVHLVEDKIHARSTGPYSLITQQP
                     LGGKAQFGGQRFGEMEVWALEAYGAAHILQELLTVKSDDVVGRVKTYEAIVKGENVLE
                     PGVPESFKVLVKELQSLGLAVEVLNEEDELVSFRAEEQADIPQIGINLSGFEREDLFS
                     SDNI"
     gene            5445..9902
                     /gene="rpoC"
                     /locus_tag="VIM45_00115"
     CDS             5445..9902
                     /gene="rpoC"
                     /locus_tag="VIM45_00115"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEY8819723.1"
                     /translation="MLEVNDFNAVRISLASPSQIRSWSYGEVTKPETINYRTLKPEKD
                     GLFCEKIFGPTKDFECYCGKYKRVRYKGIKCDKCGVEVARSKVRRERMGHIELASPVS
                     HIWFVKGTPSRLGLLLDISPRSLERVLYFAQYIIVSVNEEARQRRLNEIAAHMESDVA
                     AATATIQAQIVAARDAVDAAVGELEGERAQQLEQIEIERTAALLALETEHAAILKALA
                     KLVGKAAKKDLKLGDVTLVRTDEMVKPTFEDEVRKAYADLLDQTETGFAQRRQDIAFS
                     TDAALDQRRAAATAEVEPLVEKRKTEETAIREKHQEQIDELTREIQTLRLIPETKYRE
                     WTEQYRGVFEAGMGAEAVLRILEGVDMEDLRTRLHVEVSSTSGQRRKKATKRLRVVEA
                     LRQSGNDPSWMVFTVLPVLPPDLRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLLE
                     LGAPEIIIRNEKRMLQEAVDSLIDNGRRGRAVSGSGNHKLKSLSDMLKGKQGRFRQNL
                     LGKRVDYSGRSVIVVGPDLKLHQCGLPKRMALELFKPFVMHALVARGLAHNIKSAKRF
                     AERARPEVWDVLDEVIKQRPVLLNRAPTLHRLGIQAFEPVLIDGSAIQLHPLVCLAFN
                     ADFDGDQMAVHVPLSREAVAEARQIMLSTHNLLSPSSGDPVVAPRLDMVLGCFYMTMV
                     DPHANGRFREGDGPKRRSAVGRYGSFSEVKMAYNLGIVDLRAEIMVRDERTEHQEIKT
                     TPGRIIFNDILPFPEIEYKNVLMNGKNLKDTVAECYKKLGDERTVEIVDAIKSVGFRY
                     ATQSGITMSIHDVKVPPEKAELLRQAEENIELIDQQFQMGNLTEDERYSQAIAVWNDT
                     TARVTAEIENHFEEYGSVYIMANSGAKGNIAQIRQMAGMRGLMTDPSGRIIDLPIRSS
                     FREGLTVLEYFISTHGARKGLADTALRTADSGYLTRRLIDVAQDVIVLAEDCETLSGI
                     WLAEPADKSVMESLEERIIGRYAAQPFVNEETGEILVDRNEEIDEEKARAIVHERDRR
                     RAAGAKTGDPNVRTQVYVRSPLTCDSKRGICRLCYGRSLARGKLVEIGEAVGIIAAQS
                     IGEPGTQLTMRTFHLGGVAGGLDITSGLPRVEELFEARVPKGEAKISEIDGVAAIVRD
                     GEQRKVRISSTERYHDDYDLPAGIEAVVEDDQWVEAGTLLARPSGDAATTDVAAGGEF
                     VARFAGRVLRERGDKLSVIYEEKDEREYPIAAQARLLVEEGNVVHAGDKLTEGSKNPQ
                     DVLAIQGREAVQMYLVEEVQKVYRSQGVNINDKHIETIARQMLRKVIIDQAGDTGMLP
                     GELVDRFVYEDRNAGNLAEGGEPATAKPVLLGVTKASLSTDSFLAAASFQETTRVLTE
                     AAITGRTDHLLGLKENVIIGKLIPARATLDLPPVERLAEPRPRLFVALEDEEGLAEGL
                     FDEPGFSAVPMHGSTIVAEDIDAESEEDIDDIDEADADDEEVEEKFDEE"
     gene            10023..11369
                     /locus_tag="VIM45_00120"
     CDS             10023..11369
                     /locus_tag="VIM45_00120"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEY8819724.1"
                     /translation="MTSKTVEPDQPGSAPRLPRPESRPPTTEPPTTPPESNPYDTAVA
                     QFNAVAERLHLSPSLAQVLRNPKRELTVHFPVKMDDGSVHVFTGYRVQHNDARGPVKG
                     GIRYHPSVSIDEVRALAMWMTWKCAVVNLPFGGAKGGVCVEPKQLSLRELENLTRRYA
                     AEIGIIIGPERDIPAPDMGTDMRVMAWIMDTFSMNLGHSVPGVVTGKPISIGGSQGRV
                     EATGRGLLYITQEAQRSRGHTLEGASAAVQGFGNVGSTTARLMQAAGAKLVAVSDVGG
                     GVYNAHGFAWKDLRAAKDSDGLLKDTVRQADRITNEELLELPVDILVPAAMEGQVTGK
                     NAARVRAQLIVEGANGPITPEADAILNDNGIAVIPDILANAGGVVVSYFEWVQDLQSF
                     FWEEDEVNVKMHRIMTRAFNEVQAIQERECVTMREAANMLGVKRVVEAVETRGIFP"
     gene            11413..>11929
                     /locus_tag="VIM45_00125"
     CDS             11413..>11929
                     /locus_tag="VIM45_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018286753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HEY8819725.1"
                     /translation="MATQIKFGTDGWRAIIAEDFTFANVRALAASVARLLKQTGMADR
                     GLVVGWDTRFGSDRFAASVAQVVTAAGIKVYLGSTFAPTPACSRAIIKFGAGGGVVIT
                     SSHNPAEWNGFKYKPEYAGSASPEIVEQLEAPLDEILASGEPPQLPLEDAERKGLLQR
                     CDFRPDYLNQIA"
BASE COUNT         2456 a         3601 c         3761 g         2111 t
ORIGIN      
        1 cgggtgagcg cccgcgcgtg taacgcgcac ccctccccgt gtcatgctcc tgcacacgac
       61 ggaccacgtc ggtgctgatg cccgtgtaga gcgtgccgtc gcggcagcgg agcaggtaca
      121 actgccagcg gtgcgcagcc aaggccgttg cttctcgatc agttgtcatg aaatcaccgt
      181 agcattccgt ttttcgtccc ggacactcga ctctcggact gcttttcgat acgaccatat
      241 gcgatacttc gggcggcggt ccgactgttt cccagcaccg gcgggagtga aacgttggta
      301 ttcagacgac gcaggccgga cgctcaggaa ggcccgcagc gtggcccggg cgggggcaaa
      361 gtacgcgcga aacttagcca acgggggccg agacgccggc gaatgagcac cggggaagcg
      421 atgttcctcg gcttcctttc cggcgtgatt atctttttcg tagaacactt ttgggtccac
      481 gcgtcatggg gactcagcag cattatcgga gtgggtgtcg cagtagcttc gactgcgctc
      541 aatgtctggc tcgagtccag gcgccgaagc aagtttgcgg ccgaggtcaa agaggctcgg
      601 cggtcgcggc ggcgctgagg tcctgcgtgt attcccgatc cggcagcgct gtcgcctcaa
      661 ggccagagca gataatgacg aacgtggagg agttggcgca gagcaccgat ctggcgagca
      721 tgcggaatga actggccgcg ttgttacagg aacgctgctt ctcatacggc tatcaatatc
      781 aggctctcta tcaggcggat tcgtcgggca agctccaggc cgcttcgggc atagccggcg
      841 cggccggccg aatcgactag cacgttctac cggtgtgtga tttgcaccgt acgagcgtgg
      901 tgacgaacga ctccgaatag ttcttcgagc tcaaacggct tgggcaagta cgccgcgacg
      961 gccttgtcgg cacgatcctc ggccgacggc cggctgccgg acatgatgac gcacggaatg
     1021 gcgtcgcccc acaattcatt cacgcgtgcg gccagatctc gtccaccgag tgcgggcatc
     1081 cctacgtcca tcaagatcag tgacggcgga tcccgttcga tgacgggcaa tgcttgcatg
     1141 ccgtcgccag ccgtcactgt ccgatagcct tgctcagata gcgccacgga aatgaggtcg
     1201 caaatctgcg ggtcatcgtc gatgatcatg accagcccgg cgttcgtctg cgggcccgta
     1261 tctaccgaca tagacgcgtc ctcgaacagc ggctcttcgc cggtcaccgt atcatcaatc
     1321 gagccgcttg tggggcttga cacggtctgt atgataacag ttgaaccggc ccaggctttg
     1381 gcaaaactca gccgcggccc actatcaatg gcgttacaat ggcgcctcga agttcgttgg
     1441 cgttgatttt gcgtgctcgc cctgctacac tctggaagct gggctaatct tcgttgcctg
     1501 cctttccctc agaatacgca ttgcgaactg accgccttat ttttatctct ttttgttggc
     1561 cgcgggataa tacctttccg aggagtgctg ctctatgacc agtgccaggg accttgcacc
     1621 ggtgcgcgcc gctgtaccga gcgttcgaaa agtatatagc aagattcgag agatcatctc
     1681 ggtacccaac ctcatccagg tacagctcga ttcgtttcta tggttcaccg gcgctaagga
     1741 cgctgcgagc aaggccgaga gcggcctacg cgagcttttc gacgaaatct ctccgatcgt
     1801 cgactttacg ggctcgcgca tggagctacg gttcctcgac tacgagttcg gcgagccgaa
     1861 gtacagcgag ctcgaatgcc gcgataagga tatgacctac tcggcgccgc ttcgcgtcga
     1921 cgtcgagctc cgcgtcaaag agaccgagga gatcaaggaa caacagctat tcatgggcga
     1981 tttcccgctc atgaccaaga acggcacgtt catcatcaac ggcgctgagc gcgtcgtcgt
     2041 gtcgcagttg gtccgctcac ccggcgtcta cttcacagtg gaacaggatc cgtccagcgg
     2101 ccgcgacatg accggcgcca agctgatccc caaccgcggc gcctggctgg agttcgaaac
     2161 aagcaacaag gatatcgtct cggtcaaagt agaccgcaag cgcaagatcc cgatcacgac
     2221 gctgctgagg gcgattgacc gcgacccggc gatcgacgac gaaaccaagc tcggcaccga
     2281 cgagcgcatc ctcaccaagt ttgaggacgt cgagtcgctg tcggagatcc atcactacat
     2341 tcggaccacg ctggagcgtg actcggcccg caccaagcga caggcactcg aggagttcta
     2401 caagcgtctc cgcccgggtg acccgccgac gctggaaaac gcgcgccagt tgctcgattc
     2461 gctgttcttc aacccgcgcc gctacgacct cggtcgcgtc ggtcgctata agctgaacaa
     2521 gcgccttggc gtgtctgtcg atcagaagac gcgcacgctg acccacgacg atatcgtgag
     2581 catcgtcaag caactgatcc gcatcaactt gggtcagggg ctgcccgacg acatcgacca
     2641 tctcggcaac cgccgcgtcc gcgccgttgg cgaattgatt cagaaccagt tccgcatcgg
     2701 cctgctgcgc atggagcgcg tggtgcgcga gcgcatgacc atcaccgacc ccgatcaggc
     2761 aacgccgagc gcattaatca acatccgtcc cgtcgtcgcc gccatgaagg agttcttcgg
     2821 cggatcgcaa ctgtcacagt tcatggatca gacgaaccca ttggccgagc tgactcacaa
     2881 gcgacggctg tcggcgctgg gccccggtgg cctctcgcgc gatcgcgccg gctttgacgt
     2941 gcgcgacgtg catcacagcc actacggacg tatctgcccg atcgagactc cggaaggtcc
     3001 gaacatcggc ctgattggct cgctggcgac gtttggcaag gtgaacccat atggcttcat
     3061 cgaaacacca tatcgcaaag tcctgcgcga gctgccgaag gacagcgtcg acctcgtcgg
     3121 acacacactg cgcgaagatt tgcgcgacgg caagggcgtt atcgccgctg ccggtacgga
     3181 ggtctcgcgc gctctcgcga agaaaatcgc atccaacggc gtcgagcggg tcaaggtgcg
     3241 gccgttcgta tccgatatcg tcgaatacct ttcggccgac gtcgaagaca attacgtcgt
     3301 cgcgcaggct aacgtggccc tggacgacca gggccagttc ctggaaaccc acgtggaggt
     3361 tcggcaccga caggacttcc gggtcgagtt ggccgaccat gtggactaca tggatgtatc
     3421 gcccaagcaa attgtctcgg tggcgacgtc gctgatcccc ttcctggagc acgacgacgc
     3481 caaccgtgcg ctcatgggct cgaacatgca gcgccaggca gtgccgctcc tccggcctga
     3541 agtgccgctc gtcggcaccg gcatggaaaa gcagaccgcg tttgactcgg ggcaggtggt
     3601 cttcgcccat aaagacggcc gggtggtgag ctccacggcg aagcggatca tcgtcgagta
     3661 cgtggacgga gagcgcgaag agttccggct gcagaagttc gttcgctcga atcagggcac
     3721 atgcatcaac cagcgcccca tcgtcgagcg gggacagcag gtgattgccg gccaggcgct
     3781 ggccgacagc tcatccaccg accagggcga actggcgctc ggccagagcg tgctgtgcgc
     3841 gtttatgagc tgggagggtt acaacttcga ggacgcaatc atcatctccg aggctatttg
     3901 ccgcgaggac aagttcacgt ccattcatat tgagaaacat gagatcgaag cgcgagacac
     3961 caagctcggc cccgaagaga tcacgcgcga cattcccaac gttggcgagg atagcctccg
     4021 cgacctcgac gaggacggcg tcattcgcgt cggcgctgaa gtacgcgagg gcgacattct
     4081 ggtcggcaag atcacgccca agggcgagac cgagttgacc gcggaggaga agctgttgcg
     4141 ggcgatcttc ggggagaagg cgcgcgaagt gaaggatact tccctgcgcg tgccccacgg
     4201 cgagcgcggc aaggtcatcg acgtgaaggt ctttgaccgc gagtcgcacc tggagctccc
     4261 ggccggcgtg aacaagctgg tacgggtttc gatcgcgcaa aaacgcaagc tgtccgaagg
     4321 cgacaagatg gctggccgcc acggtaacaa gggtgtcgtc gcccgtatct tgccggtcga
     4381 agacatgccg tacctggcag atggccggcc ggtagacatc gtactgaacc cgatcggcgt
     4441 gccgagccgc atgaacctgg ggcaggtgct cgagacgcac ttgggctggg cggcgaagac
     4501 gctcggattc cgggcgatca cgccggtgtt cgacggcgcc agcgacgtca agatcgaaga
     4561 tgcgttggcc cgcgcctggc tgacaatgga ggccggtgcg gtatcgctga atcccatttc
     4621 cgggcagacc acgaccaaga ctgaggacga ggtcggcgaa tatatcgacc tcgaacggct
     4681 tcgcgagtgg ctcggcgagc gcggctataa ccccgacgaa gttttcgacg actcgcatcc
     4741 cggcgccgcg agacgggcct gtctcgagct ctggatggaa caggttggcg aaaagaaggt
     4801 gagaggtcgc tcgctcgagg agctcgacga actggccgat cggatgagcg ccgaccgccg
     4861 catggcgccg ccgatcttcg gcaagcaggt gctgtacgac ggccgctccg gcgaggcgtt
     4921 cgaccagccg gtcacgatcg gctacatcta catgctgaag cttgtccacc tcgtggaaga
     4981 caagattcac gcacgttcca ccggtccgta ttcgctcatc acccagcagc cgctgggtgg
     5041 aaaggcgcaa ttcggcggtc aacggtttgg tgagatggag gtttgggctc ttgaggccta
     5101 tggcgccgcg cacattctgc aagaactgct gacggttaag tcggacgacg tagtgggacg
     5161 tgtcaagacc tatgaagcga tcgtcaaagg cgagaatgtg ctcgagcccg gcgtgccgga
     5221 atcgttcaag gttctcgtca aagagctgca gagcctcggg ctggcggtgg aggtgctgaa
     5281 cgaggaagac gagctcgtct ccttccgggc agaggaacag gccgacatcc cgcagatcgg
     5341 aatcaacctc tccggtttcg agcgcgaaga cctcttctcc agcgacaaca tctaacaact
     5401 aacaacccgt attaacccgg ccatgggtgg aggaaattcc ttacatgctg gaagtcaacg
     5461 attttaacgc cgtccgaatc tccctggctt cgccgtcgca gattcgcagt tggtcctacg
     5521 gcgaggtcac gaagccggaa acgatcaatt accgcacgct gaagccggaa aaggacggcc
     5581 ttttctgcga aaagatcttt ggtccgacca aggatttcga gtgctattgc ggcaagtaca
     5641 agcgcgtccg ttataagggc atcaagtgcg acaagtgcgg cgtcgaggtg gcgcggagca
     5701 aggtccgccg cgagcgcatg gggcatatcg agctcgcgtc gcccgttagc cacatctggt
     5761 tcgtgaaggg cactccgagc cgccttggtc tgttgctcga tatttcgccg cgctcgttgg
     5821 agcgagtact ttacttcgcc cagtacatca tcgtgtccgt caacgaagag gcgcgacaac
     5881 ggcggctgaa cgaaatcgct gcacacatgg aaagcgatgt ggccgccgcg accgcgacga
     5941 tccaggcaca aatcgtggcg gcgcgcgacg cggtcgatgc ggcggtcggg gagctcgaag
     6001 gcgagcgcgc ccagcagtta gagcagattg agatcgagcg caccgccgcg ctactggcgc
     6061 tcgaaacaga gcacgcggca atcctcaagg ctctggcaaa attggttggc aaggctgcga
     6121 agaaggactt aaagctcggc gatgtgacgc tggtccgcac cgacgagatg gtcaagccga
     6181 cgttcgaaga cgaggtccga aaggcctacg ccgacctgct cgatcagacc gaaacgggct
     6241 tcgcccagcg ccgccaagac atcgccttct cgactgacgc ggcgcttgac cagcgtcgtg
     6301 cagcggccac cgcagaggtt gagccgctgg tcgagaagcg caagaccgaa gagaccgcga
     6361 tccgtgagaa gcaccaggag cagatcgacg agctgacccg agaaatccag accctgcggc
     6421 tgatcccgga aacgaagtat cgcgaatgga ccgagcaata tcgcggcgtg ttcgaggctg
     6481 ggatgggcgc ggaggcggtc ctgcgcatcc tcgaaggtgt cgacatggag gatctgcgga
     6541 cacggctgca cgttgaagtc agctcaacca gcggccagcg gcgcaagaag gcgaccaagc
     6601 gactccgcgt cgtggaggcg ctgcgccagt cgggcaacga tccatcatgg atggtattta
     6661 ccgttctacc ggtgctgcca ccggacctac gtccgatggt gcagctcgat gggggccgtt
     6721 tcgctacgag cgacctgaac gacctgtacc gccgcgtgat caatcgcaac aaccgcctga
     6781 agcgtctact cgagcttggc gcccccgaga tcatcatccg caatgagaag cgcatgcttc
     6841 aggaggcggt cgactccctg atcgacaacg gccggcgcgg acgggcggta tccggatctg
     6901 gcaaccacaa gctgaagtcg ctctccgata tgctcaaggg caagcagggg cgcttccgcc
     6961 agaacctgct gggcaagcgc gtcgactact ccggccggtc ggtgatcgtc gtcggtcctg
     7021 acttgaagtt gcaccagtgc gggctgccga agcgcatggc gcttgaactg ttcaagccgt
     7081 ttgtcatgca tgccctggtc gctcggggac tggcgcacaa catcaaaagc gcgaaacggt
     7141 tcgccgagcg ggcacgtccc gaggtctggg acgtgctcga cgaggtgatc aagcagcgtc
     7201 ccgtgctgct caaccgcgct ccgacgctcc accgcctcgg catccaagcc ttcgagcccg
     7261 tgctgataga cggaagcgcc attcagctcc acccgctggt gtgtctcgca tttaacgccg
     7321 actttgatgg cgaccagatg gcggtgcacg tgcccctatc acgcgaggcg gtggctgaag
     7381 cccgccagat catgctgtca acgcataacc ttctatcgcc cagctcgggc gacccggttg
     7441 tggcgccgcg gcttgatatg gtgctcggct gcttctatat gaccatggtg gacccgcacg
     7501 ccaacggccg attccgtgag ggcgatggtc ccaagcggcg ctcggcggtc gggcgttacg
     7561 gcagcttctc cgaagtcaag atggcctaca atctggggat cgtcgacctg cgcgcggaga
     7621 ttatggtccg cgacgagcgg accgaacatc aggagatcaa gacgaccccg gggcggatta
     7681 ttttcaatga catcctcccg ttcccggaga tcgaatacaa gaacgtcctg atgaacggca
     7741 agaacctgaa ggacacggtc gcggaatgct acaagaagct gggcgacgag cgcacagtcg
     7801 agatcgtcga cgcgatcaaa tcggtcgggt tccggtacgc gacccagtcc ggcatcacca
     7861 tgtcgattca cgacgtcaaa gtaccgccgg aaaaggctga gctgctgcgc caggccgagg
     7921 agaacatcga gctcatcgat cagcagttcc agatgggtaa cctgaccgaa gatgagcggt
     7981 attcccaggc gattgcggtg tggaacgaca ccacggcgcg ggtgacggct gaaatcgaaa
     8041 accacttcga ggagtacggt tcggtctaca tcatggcgaa ctcgggggcg aaaggtaata
     8101 tcgcgcagat ccgccagatg gccggtatgc gcggattgat gaccgaccct tcggggcgca
     8161 tcatcgacct tccgattcga tcgagcttcc gcgaggggct gacggttctc gaatacttca
     8221 tctcgaccca cggcgcccgt aagggtctgg ccgacaccgc cctccgtacg gcggactcgg
     8281 gttatctgac tcggcgcctg attgacgtgg cgcaggacgt catcgtcctc gccgaggact
     8341 gcgagacgct ctctggcatc tggctcgcgg agcccgccga taagagcgtg atggagtcct
     8401 tagaggagcg aatcattggc cgctacgcgg cccaaccatt cgtgaatgag gagacgggcg
     8461 aaatcctcgt cgaccgcaat gaagagatcg acgaagaaaa ggcccgcgcc atcgtccacg
     8521 agcgcgaccg gcgtcgagcc gcaggagcga agacaggcga tccgaacgtt cgcactcagg
     8581 tttacgtgcg gtcgccgctg acttgcgact ccaagcgtgg catttgccgc ctctgctacg
     8641 ggcgcagcct ggcccgcggc aaactagttg agatcggcga ggcggtcggg atcatcgccg
     8701 cacagtcgat cggcgagccg ggtacgcagc tcaccatgcg tacgttccac ctcggcggcg
     8761 tcgccggcgg cctggatatc accagtggcc tcccgcgcgt cgaggagctg ttcgaggccc
     8821 gggtgccgaa gggcgaagca aagatatcgg agatcgacgg cgttgccgcc attgttcgtg
     8881 acggcgaaca gcgcaaggtg cgaatctcga gcacagaacg ctaccacgac gactacgatc
     8941 tgccggccgg tatcgaagcc gttgtcgagg acgaccagtg ggttgaagcc ggtacgctgc
     9001 tcgcgcggcc aagcggcgac gccgcgacga ccgacgtcgc ggccggtggc gagttcgttg
     9061 cacgttttgc cgggcgtgtg ctccgcgagc gcggtgataa gctctcggtc atttacgaag
     9121 aaaaggacga acgcgagtat ccgatcgcgg cgcaggcgcg gctgctagtc gaagagggca
     9181 acgtcgttca cgcgggtgac aagctcaccg aaggctcaaa gaacccgcaa gacgttcttg
     9241 ccatccaggg gcgcgaggct gtacagatgt acctcgtcga agaggttcag aaagtctacc
     9301 ggtcccaggg tgtgaacatc aacgataaac acatcgagac gatcgcacgt cagatgctcc
     9361 gcaaggtgat catcgaccag gctggtgata cgggcatgct gccgggcgag ctcgtggacc
     9421 gattcgttta tgaggatcgc aacgcgggca acctggcgga aggcggcgag cccgccaccg
     9481 ccaagccggt gctgctgggt gtgaccaagg cgtcgctcag cacggatagc ttcctggcgg
     9541 cggcgtcgtt ccaggagacc acccgcgtcc tgactgaagc cgcaattacg ggccgaacag
     9601 accatctact gggcctgaag gagaacgtga tcattggcaa gctcataccg gcgcgggcca
     9661 ctctcgacct gccgccggtg gaaaggctcg ctgagccacg cccgcgcctc ttcgtcgccc
     9721 tcgaagacga agaaggactg gcagagggtc tcttcgacga gccgggcttc agcgcagtgc
     9781 cgatgcatgg ctccacgatt gtggccgaag acatcgatgc cgagagcgag gaggacatcg
     9841 acgacattga cgaagccgat gctgatgacg aagaggttga agaaaagttt gacgaagagt
     9901 agtccttgag gtgcgtaatt ctgtaggagc cggcggtaat ccgccggctc ctttgtttcc
     9961 cgggacgccg ggccagctat gctaagatcg cagtagcaca acaggcagca tcgaggacgg
    10021 taatgaccag caagacggtc gaacccgacc aaccagggtc tgcaccccgc ctcccgcgtc
    10081 ccgagtcccg cccaccgacg actgaaccgc ccaccactcc accggaatca aacccgtacg
    10141 acaccgccgt ggcccaattc aacgccgttg ccgagcggct gcacctttcg ccgagcctcg
    10201 cgcaggtgct gcgtaacccc aagcgcgagt taaccgttca tttccccgtg aagatggacg
    10261 atgggtccgt gcacgtcttc accggttacc gcgtgcagca caacgacgcc cgcggaccgg
    10321 tgaagggggg cattcgctat catccctctg tatcgatcga cgaagtgcgc gccctcgcta
    10381 tgtggatgac ctggaagtgt gcggtggtga atctaccgtt tgggggagcc aagggcggcg
    10441 tttgcgtcga accgaagcag ctttcgttgc gtgaattgga gaaccttacc cggcgctatg
    10501 cggccgaaat cggcatcatc atcggccccg agcgcgatat cccggcgccc gatatgggga
    10561 cggacatgcg agtcatggcg tggatcatgg ataccttttc catgaacctt ggccattccg
    10621 ttcccggagt cgtgaccggc aagccgatca gcatcggcgg gtcccagggc cgcgtcgagg
    10681 caacagggcg cggtctcctc tacattacgc aagaggcgca gcgaagtcgc ggacacacgc
    10741 tcgaaggcgc ctcggcagcg gtccagggat ttggcaacgt cggctctacg acggcgcggc
    10801 tgatgcaggc cgcgggcgcg aaactcgtgg cagtgagcga cgttggcggg ggcgtctaca
    10861 acgcacacgg gttcgcatgg aaggatctgc gcgctgccaa ggatagtgat ggccttctga
    10921 aggatacggt tcgccaggcc gatcggataa cgaacgagga gttgctcgag cttccggtcg
    10981 atatcctggt gccggccgca atggaaggac aggtcaccgg aaagaacgcc gccagggtcc
    11041 gcgcccaact gatcgtcgaa ggcgcaaacg gaccgatcac gcccgaggcc gatgcgatcc
    11101 tcaacgataa cggcatcgcg gtcatccccg acattctcgc caacgccggc ggtgtggtcg
    11161 tctcatattt cgaatgggtg caggacctcc aatccttctt ctgggaagaa gacgaggtga
    11221 acgtgaaaat gcaccgcatc atgacgcgcg ccttcaacga agttcaggcg atccaggagc
    11281 gggagtgcgt cacgatgcga gaggcggcga acatgctggg cgtcaagcga gtggtcgagg
    11341 cggttgagac gcgcggcatc ttcccttgac ggatcaggac cgcgccttat ccgccacgat
    11401 tgggagaaac gcatggccac ccagatcaaa ttcggaaccg acggctggcg ggcaatcatt
    11461 gctgaagact ttacgtttgc caacgtccgc gcccttgcgg cttcggttgc gcggctcctg
    11521 aaacagaccg gtatggccga ccgcggactt gtggtcggct gggacacccg cttcggttcg
    11581 gatcgcttcg ccgccagcgt ggcacaggtg gtgaccgccg cgggcatcaa ggtctacctg
    11641 ggaagcacct ttgcgcccac tccggcctgc tcgcgcgcca ttatcaagtt cggggctggc
    11701 gggggcgtgg tgataacgtc gagccacaac ccggccgaat ggaacggatt caagtacaag
    11761 ccggaatatg ccggctccgc gtcaccggag atcgtcgagc aactggaagc gccgctcgac
    11821 gagatcctgg cctctggtga gccccctcag cttccactcg aagatgccga gcggaaggga
    11881 ctcctccagc gttgcgattt ccggcccgac tatctgaacc agatcgcgt
//
LOCUS       DATCPE010000006         5945 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U7219
            k141_194920, whole genome shotgun sequence.
ACCESSION   DATCPE010000006 DATCPE010000000
VERSION     DATCPE010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955033
            Sequence Read Archive: SRR1586279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 5945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 16:49:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 640
            CDSs (total)                      :: 631
            Genes (coding)                    :: 627
            CDSs (with protein)               :: 627
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5945
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194920"
                     /isolate="SMAG_U7219"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..654
                     /locus_tag="VIM45_00130"
     CDS             <1..654
                     /locus_tag="VIM45_00130"
                     /inference="COORDINATES: protein motif:HMM:NF014510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase"
                     /protein_id="HEY8819726.1"
                     /translation="IVFQPEIRRALERLGRTRVASRRSRRASDVVLGALVQASAELAM
                     RRLGALIVIERKTGLDDYIRTGILIEGIPTAALVENLFWRNSPLHDGAVIVRENRLIA
                     AACTLPLSESPMPGHLGTRHRAALGLSERTDAIVVVVSEESGSISLVVDGQLIGGLDA
                     DGLRALLRNLIGQSVDEPLRLIRSHDRAYETERMSPDETSSTAADTDRNISAGTGGV"
     gene            661..1998
                     /locus_tag="VIM45_00135"
     CDS             661..1998
                     /locus_tag="VIM45_00135"
                     /inference="COORDINATES: protein motif:HMM:NF019561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HEY8819727.1"
                     /translation="MDQKRSNGYIARVREARRFLTEGGRTGVRSLRSNSPLMLLSLGL
                     AIVLWAFVTNQENPSLRKDVSFQVSSFDPVNVPKGMLVTRETPNDITVTLIGPRTAVN
                     DVRTEDIQINVDLSGADSNVPGGGAVVYTAPVKASVKQRGGGRVRAQLDPTDVRVTLE
                     PVVRRSVSVRINSTDAPPIGFELESQPVADPSEATVSGLRQNVDAVDAVYADLKLTGL
                     SVSTTVALPLSARSSDGRAISGVTVQPSTASIKVAIKRTVFNREVFVSVQTHGKPAVG
                     YQEVEAHSDPPSVTIAGSLDTLNNVTTIPTDDVEIEGATQDVKRVVGLKPPPGVTVVS
                     SRTVAATITIAPGRGQGQMLVAPRVIGLAAGVTAQISTTALVVTFAGPQPQLLALKPA
                     DVTATVDVSGLAPGTYSLDPKIGLPQGIDKDSVTPAKVDLVIVSSGSPSTPAR"
     gene            2067..3428
                     /gene="der"
                     /locus_tag="VIM45_00140"
     CDS             2067..3428
                     /gene="der"
                     /locus_tag="VIM45_00140"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEY8819728.1"
                     /translation="MTNEIVSLVSPTANVELPVVAIVGRPNVGKSALFNRLVGGRVAL
                     VEDLPGTTRDRIYGLVTWPKRPFRVVDTGGLEEGSSELYPALIRSQIEQALREADAVL
                     FAVDARDGLTAADYEVADILRRSHKPVVLVANKADNLQRRQEIGQFYELGLGDPLPVS
                     AYHDSGINDLFDALELLLPEAQLPAGTPALGISIVGRPNVGKSALLNAILGEQRVIVS
                     DVPGTTRDAVDTTFSYAGRDLVLIDTAGIRRRGHIEPGVEKHSVMRARAAIERADVAF
                     VVMDANELLTAQDTHIINFVEEAVKSMVIVVNKIDLLKLEGEWKADLTRLIRHRLKFA
                     PWAPIAFVSAMKKTGIEEMLRVAIDAGDARNQRVPTAALNSLLKRAITKHSPPSVGGK
                     RLNILYVTQAEVRPPTFVIFVNDPALVHFSYERYLENTLRDAFGFPGTPVRLIFKQRG
                     GAE"
     gene            3425..4072
                     /gene="plsY"
                     /locus_tag="VIM45_00145"
     CDS             3425..4072
                     /gene="plsY"
                     /locus_tag="VIM45_00145"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEY8819729.1"
                     /translation="MIWQVSLIAVLGYLCGAIPSGLWVGKATRGIDIREYGSGKTGFT
                     NSVRTLGKVPAMAVLVVDVVKGAVPVLVARVLFDGAPIQAVAGIAAMAGHIWPIYVGF
                     RGGRGVLVAFSATTAMMPLLGPLMIAIGLAIALPFGYISLASIVGSGVMAGIIVALAL
                     AGRVPAAYAVFGAAAATMIALLHRDNIQRLRAGTEPKFGDGGNRRTDPGAANSHP"
     gene            4072..5031
                     /locus_tag="VIM45_00150"
     CDS             4072..5031
                     /locus_tag="VIM45_00150"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEY8819730.1"
                     /translation="MLNDTKLRAILNPTQPSRTAGLTGLSNLDEDESAELRRVVFDVS
                     VERRRWVAQSLNDLAEDNATFDFEAVFTILLDDEDPHVRRLAIDGLWEVDDRRLNEPL
                     IRMLQGDPDEGVRATAALALGRYVVLNEFGEVRARDADSVIDALRGAIDDPNQRLEVR
                     ARAVEAAGASSAAWAEDMIWRAFDADEPRFQVSAIHAMGRNANPDWLPTVFTEMESSD
                     PQRRFEAAGAAGRIGDEDAIPHLVDLIEDEDAEVQEAAIAALGDIGGDAAVAVLRSHL
                     QNADARIQDAIRTAIAEALAAEGLAGPPQIRPVGDDDEDDDYE"
     gene            5053..5562
                     /locus_tag="VIM45_00155"
     CDS             5053..5562
                     /locus_tag="VIM45_00155"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEY8819731.1"
                     /translation="MVRRSGQRLRIEDIVSSGGIVYRVSESGPEVVLCGRSLERIWGL
                     PKGTPQPDETLEQTAIREVSEETGLEVEIERKVGEIEYWFSRVEQGVRFHKRVHHYLM
                     VPTGGDTANHDNEYDVVRWFPLDEAERLLTYGNEAEILRRAAPLIAVEANGRMSDGKP
                     EAAGGRGQS"
     gene            5559..>5945
                     /locus_tag="VIM45_00160"
     CDS             5559..>5945
                     /locus_tag="VIM45_00160"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8819732.1"
                     /translation="MTLEIEDEVARNGLIRLRRKRREDADADYQWRCDPELARYDAAT
                     PLRASLSDFLLTYEEDLRFPSPFRRVFAIESLDGQHIGNVMYYNIDERRGEAELGITI
                     GERQYWSQGYGRDAVSAFVRFIFSSSS"
BASE COUNT         1171 a         1732 c         1910 g         1132 t
ORIGIN      
        1 atcgtctttc agccggagat ccggcgggcg ctggaacgcc tggggcgcac gcgcgtggcg
       61 tctcggcgca gccggcgcgc ctcggatgtg gttctcggcg cccttgtgca ggcgagtgcg
      121 gaacttgcaa tgcggcggtt gggagcgctg atcgtcatcg agcgcaaaac cgggctggac
      181 gattacatcc gaaccggtat cttgatcgag ggcataccga cggcggcgct tgtggagaac
      241 ctcttctggc ggaactcacc actccacgat ggcgcggtca tcgtgcgaga aaatcggctg
      301 atcgctgcgg cctgcacgtt gccgctctct gagagcccga tgccaggcca tcttggaacc
      361 cgtcatcggg ctgcacttgg attgagtgaa cgaacagacg cgatcgtcgt cgtggtctcc
      421 gaggaatccg ggtcgatctc actggtggtg gatggtcagc tcatcggtgg tcttgatgcc
      481 gacgggttgc gggcgctgtt gcgcaacttg atcggccagt cggtggacga gcccctgcgc
      541 ctcatccggt cgcacgatcg ggcctacgag acggaacgaa tgtcgcctga cgagacctcc
      601 tcgacggccg cagatacaga ccgcaacatc tctgcgggca caggcggagt ttagacggcg
      661 ttggaccaga agagatcgaa tggatacata gcgcgtgtgc gcgaggcgcg gcggttcctg
      721 acagagggcg gccgcacggg cgttcgctca ctgcgctcca actccccatt gatgctgttg
      781 tcgctgggat tggcgattgt gctgtgggca tttgtgacga accaggagaa cccgtcgctc
      841 cgcaaagatg tctcgtttca ggtttcgtct ttcgatccgg tgaacgtacc aaaggggatg
      901 ttggtcaccc gcgaaacacc aaatgacata acagtcacat taattggccc gcgaaccgca
      961 gtgaacgacg ttcgcaccga agacattcag atcaacgtcg acctctccgg cgccgattcg
     1021 aacgtcccag ggggtggcgc cgtggtctat acggcaccgg tgaaggcgtc agtcaagcag
     1081 cgcggaggcg gcagggttcg cgcccaactc gatccgacgg atgttcgagt gacgctcgaa
     1141 ccggtagtgc gccggtcggt ctcggtaaga atcaattcca ccgacgcgcc gccaattggc
     1201 ttcgagctcg agtcacaacc ggtcgccgac ccttcagagg ccaccgttag cggcttgagg
     1261 cagaacgtgg atgccgtaga cgccgtgtac gccgacctca agctgaccgg tctatcagtt
     1321 tctaccaccg tggcactccc cttgagcgcg cgcagcagcg acggtcgcgc aattagcggc
     1381 gttactgttc agccgtcaac cgcgtcgatc aaagtcgcga tcaagcgcac cgttttcaat
     1441 cgcgaagtgt ttgtttctgt gcaaacgcac ggtaagccgg ccgtcggtta tcaggaagtc
     1501 gaggctcact cggatccgcc aagcgtgacg atcgccggca gccttgacac gctaaacaat
     1561 gtcacgacga ttccgaccga cgacgtagaa attgaaggcg ctactcagga tgtcaaacgg
     1621 gtcgtggggc taaagccacc tccgggcgtg acggtcgtaa gttcgcgaac cgtcgcggcc
     1681 accatcacca tcgcgccggg tcgcggacaa gggcagatgt tggttgcgcc gcgcgtcata
     1741 ggactcgcgg ccggagtcac tgcacagatc tcgacgacgg cattagtcgt cacgttcgcc
     1801 ggaccccagc ctcagcttct tgccctcaaa ccggcggacg taaccgccac ggttgacgtc
     1861 agcggccttg cgccaggaac gtacagcctc gacccgaaga tcgggttgcc gcaaggcatc
     1921 gacaaggatt cagtcacgcc cgcaaaagtt gacttggtga tcgttagtag cggctcacca
     1981 tctacgcctg cccgctagcg agagcgatta cgccccactt ccggtagttc gcgcgccccc
     2041 tgcatccggg cggctatgat ggataggtga ctaacgaaat cgtctcctta gtatcaccaa
     2101 cagccaacgt cgagttgcct gtcgtcgcca ttgtcggccg gcccaacgtc ggcaagtccg
     2161 cgcttttcaa ccgtctcgtc ggcggcaggg tcgcactggt agaagatctg cccggcacca
     2221 cccgcgaccg gatatacggt ctcgttacct ggccgaaacg gccgttccgc gtcgtcgaca
     2281 ccggtggcct ggaagagggc agtagcgagc tatatccagc gctcattcga tcacagattg
     2341 agcaggcgct gcgggaagcg gatgccgtgt tgttcgcggt agatgcgcgg gatggtctga
     2401 cggcggcaga ttacgaggtc gccgacattt tgcgccggag ccacaagccc gtggtcctgg
     2461 tcgcgaacaa agcggacaac ctacaacgtc gacaagagat tggccagttt tacgagttgg
     2521 gattgggcga tccactcccg gtgagcgcct accacgattc cggcatcaac gatctgtttg
     2581 acgctctcga gctgctgctg ccggaagcgc agctaccggc agggacgccg gccctcggca
     2641 tctcaatcgt cggccgtcca aacgtcggca agtcggccct gctcaacgcc atactcggcg
     2701 aacagcgagt aatcgtcagc gacgtaccgg gtacgacgcg tgacgcggtc gataccacgt
     2761 tctcatacgc cggccgggat ctcgtgctga tcgatactgc aggcattcgc cggcgcggac
     2821 acatcgaacc gggcgtcgag aagcacagcg tcatgcgggc acgggccgcg atcgagcgcg
     2881 ccgacgtcgc ctttgttgtc atggacgcca acgagctgct caccgctcaa gacacgcaca
     2941 tcatcaactt cgtcgaggag gcggtgaagt cgatggtgat cgtcgtgaac aagatagacc
     3001 tgctcaaact cgagggcgag tggaaagcag acttaacgcg gttgatccgg caccggctca
     3061 agttcgcgcc ctgggcgcct attgcatttg tctcggcgat gaagaagacc ggaattgagg
     3121 agatgttgcg cgtcgcgatc gacgccggcg atgcccgcaa ccagcgcgta ccgactgcag
     3181 ctctgaattc gttgttaaag cgtgcaatta caaagcacag cccgccttcc gtcggtggca
     3241 agcgacttaa tatcctgtat gtgacgcagg cagaagtccg tccaccgacg ttcgtgattt
     3301 ttgtcaacga ccctgcgctg gtgcatttct cgtacgagcg gtaccttgag aacaccttgc
     3361 gcgacgcgtt tggcttccct ggtacaccgg tccggctcat tttcaaacag aggggcggcg
     3421 ccgaatgatc tggcaggtct cgctaattgc ggtgctcgga tacctgtgcg gcgctatccc
     3481 gtcgggtctg tgggtcggaa aggctacacg cggaatcgac atccgtgagt acgggagcgg
     3541 caagaccggc tttaccaaca gcgtccggac gctcgggaag gttcccgcta tggcagttct
     3601 tgtcgtcgac gtcgtcaagg gcgccgttcc ggtgctcgtt gcgcgggtcc tgttcgacgg
     3661 cgcaccaatt caggccgtgg ccgggattgc ggcgatggca ggccatattt ggcccatcta
     3721 tgtcggtttc cgcggcggca gaggtgtatt ggtggcgttc tcagcgacca cggcgatgat
     3781 gccattattg ggaccgctga tgatcgctat cggccttgcg atcgcgctgc cgtttggcta
     3841 tatctcgctg gcgtcaatcg tcggcagcgg cgtcatggca ggcatcatcg ttgcactggc
     3901 gttggcgggc cgggtaccgg ccgcatacgc ggtatttggc gcggcggcgg ccacgatgat
     3961 tgcgctgttg caccgcgaca atattcagcg attgcgggcg ggcacagagc caaagttcgg
     4021 ggatggcggt aataggcgta ccgatccggg ggcggcaaac agtcacccgt aatgctgaac
     4081 gacacaaagc tccgggcgat cctgaatcca acacaaccgt cccggaccgc ggggcttacg
     4141 ggcctctcaa atctcgatga agatgagagt gccgagcttc gtcgtgtcgt atttgatgtc
     4201 tccgtggagc gcaggcgctg ggtagcccag agcctcaacg atctggccga agacaacgcc
     4261 acgtttgatt tcgaggccgt gttcacgatc ctcctcgacg acgaagaccc gcacgttcgg
     4321 cggctggcca tcgatggatt gtgggaggtc gatgatcgac ggctgaacga gccactgatc
     4381 cgcatgttgc agggagaccc ggacgagggc gtgcgcgcta ccgccgcact tgctctggga
     4441 cggtacgtgg tactcaatga gttcggcgag gtgcgcgccc gcgacgcaga cagcgtgatc
     4501 gacgcgctgc gcggcgcgat cgacgatccc aatcaacgtc ttgaagttcg agcccgtgcg
     4561 gtcgaagcag ccggggcatc aagcgcggcg tgggccgaag atatgatctg gcgtgcgttc
     4621 gatgccgacg agccccggtt ccaggtcagc gccatccacg cgatggggcg gaacgccaat
     4681 ccggattggc tgccgaccgt attcaccgaa atggagagtt ccgaccctca acgccgattt
     4741 gaggcggcgg gcgcggcagg tcggatcggc gatgaagacg caatacccca cctcgtagat
     4801 ctgatcgagg acgaggatgc cgaggtgcag gaagctgcaa ttgcggcgct cggcgatatt
     4861 ggcggcgatg ccgctgtagc ggtgctgcgg tcgcatctac agaacgccga tgcacgtatc
     4921 caggatgcga tccgcaccgc catcgccgag gcgctcgctg ccgaggggtt ggcggggccg
     4981 ccgcagatcc gtccggttgg cgatgacgat gaagatgatg attacgagta atccaacggg
     5041 cggtgtggcg gcgtggtaag acggtcgggg cagcgtcttc gcatcgaaga catcgtttcc
     5101 tccgggggga tcgtctatcg agtaagcgaa tccgggcctg aagttgtact ttgcggccgc
     5161 tcgctggaac gaatctgggg tctgccgaaa ggcacgccgc agcccgatga gacactagag
     5221 cagacggcga tacgcgaggt cagcgaggaa acgggcctcg aggtagagat cgagcgaaag
     5281 gtcggcgaga tcgaatactg gttctctcgg gtggaacagg gcgtgcgctt tcataagcgg
     5341 gtacatcatt atttgatggt gccgacgggc ggagacaccg caaatcacga caatgagtac
     5401 gacgtcgtgc gctggttccc gctcgacgaa gctgaacgat tgctgacata tggcaacgag
     5461 gcggagatcc tgcgtcgtgc tgcgcccctt atcgccgttg aagcgaatgg taggatgtca
     5521 gacgggaaac cggaggcagc cggagggcga ggccagtcgt gacgctcgaa atcgaagatg
     5581 aggttgctcg gaacgggctc atacgattgc gaaggaagcg ccgtgaagac gccgacgccg
     5641 actaccagtg gcgttgcgac cctgaacttg cccgctatga tgcagcgacg ccgctgcgag
     5701 ccagcctcag tgatttctta cttacgtacg aggaagatct tcgctttccg agcccgtttc
     5761 gccgcgtgtt tgccatcgaa agcctggacg gacagcacat cggcaacgtg atgtactaca
     5821 acatcgacga acggcggggc gaagctgagc tgggaatcac gattggtgaa cgccaatact
     5881 ggagccaggg ctacggtcgc gatgccgtta gcgccttcgt tcggttcatt tttagcagtt
     5941 cgtct
//
LOCUS       DATCPE010000007        20508 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U7219
            k141_357009, whole genome shotgun sequence.
ACCESSION   DATCPE010000007 DATCPE010000000
VERSION     DATCPE010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955033
            Sequence Read Archive: SRR1586279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 20508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 16:49:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 640
            CDSs (total)                      :: 631
            Genes (coding)                    :: 627
            CDSs (with protein)               :: 627
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20508
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_357009"
                     /isolate="SMAG_U7219"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="VIM45_00165"
     CDS             <1..244
                     /locus_tag="VIM45_00165"
                     /inference="COORDINATES: protein motif:HMM:NF013097.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEY8819733.1"
                     /translation="VLFELPIVMMVPAKFGIIRARKYLKWWRYAIVLGFLAAAAVIPN
                     ISPIEQISVAIPIIGLYFVGVGLAKLVQPKQAAVSE"
     gene            325..939
                     /locus_tag="VIM45_00170"
     CDS             325..939
                     /locus_tag="VIM45_00170"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEY8819734.1"
                     /translation="MQTNLGELSQLIKRCPDCDLSQTRTQAVPGEGPDTAAIMFIGEG
                     PGYYEDKSGRPFVGNAGKFLDELLQHAGLKRCDVFITNVVKCRPPQNRDPLRDEIDAC
                     DKWLKHQINVIDPKVIVTLGRISMGKFFAGESISKIHGQPHKVGDRTIVPMFHPAAAL
                     HQERFRSLIVDDFKQLPEVLESVQREKAASDQPAQPSTEQGRLF"
     gene            960..2618
                     /locus_tag="VIM45_00175"
     CDS             960..2618
                     /locus_tag="VIM45_00175"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012881709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEY8819735.1"
                     /translation="MADHQTLRVIPLGGLGEIGKNMMVFELADDIVAVDCGLQFPEAE
                     MLGVDLVIPDTTYLRERLDKVRAILITHGHEDHIGALPYVLRDVNVPVYCTRLTYGLI
                     SVKLKEHRLLRESDLRIVEPGEQIQLGDFTAEFFRVAHSIPDACGIILHTPLGRVVHT
                     GDFKLDHTPVMGQHTDLPRLTELGNEGVTLLCADSTYAEIPGYTPSEQVVGVALDNIM
                     NQAEGRVIVATFASLISRVQQVIDAAVHHNRRVFVIGRSMVDNVNMALEQGYLEVPDG
                     VLGRIEEMRNLPVHRVAIVTTGSQGEPTSALSRMANRDHRWVQIVPGDTVVLSASPIP
                     GNEQFVYNVVDNLFKLGAKVLYNRVDNIHVRGHAAQEELKLLLSLVRPKYLLPIHGEY
                     RHLMLHARIGQAMGMAPEDTFVIEDGDVLEITQDGAEVAGRAPAAAVYVDGLGIGDVD
                     HVVLRDRRHLANDGMVVVVVAVDKQTGKVIDLPDVLSRGFVEASEAGELMDRVRDVAM
                     QSLDGADHRAEWPDINAKVKDTVAKFLYDETRRRPMVLPVAVEV"
     gene            2667..5048
                     /locus_tag="VIM45_00180"
     CDS             2667..5048
                     /locus_tag="VIM45_00180"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEY8819736.1"
                     /translation="MAKAKGSSRPRTSLSSPRAERRRKPAFRGDIAEMALRPITPVVA
                     FLLTPAGLATAIVLIVAAAMPWLSIVDVLDAFRRAVVQGTGVVCYLIVIDLLALVAIP
                     GIAPGPRTSRRFWRIATGFQFLGLFVWGVLGALHPETALAGVSLQDYSAGGNVGHSLV
                     ASGFGVLVWLLALSIGCALVSPSGTRWALAQLPPAARSLWALNIPGKLLAALGTLRPA
                     RTPYAVAGTSAVSLPDIEESPPPETIGQGAADAFRRTGERERQRQALAPTLELPGFGT
                     GTTAAPHGVGWQLPNTQLLSTSAPSQLSPGDNEARARTIVETLASFGVDAKVLQINQG
                     PSVTQFGVEPGWEIKTRNDPERDASGKVLLDKDNKPRMRVEEVSRTRVRVNQIQKLQN
                     DLALALAAPTIRIEAPVPGKPMVGIEVPNTSSSVVSLRSVVETPQFQKVASKTKLALA
                     LGMGVSGEPIIADLATMPHLLIAGTTGSGKSVCIRALISCILMHATPDDVRVVLIDPK
                     RVEMAVFAPIPHLALSKIVTDMEEVAGTLQGVIREMEDRYKKFEMFKVRNLEAYNRNP
                     KVAGKLPNWVVIIDELADLMMAVPYEVERQICRLAQLARATGIHLVVATQRPSVDVIT
                     GLIKANFPTRIAFAVSSQVDSRVILDGVGAEKLLGRGDMLFMPTDQAKRRVQGVYVSD
                     QEIERLVDFWGAERWEKLRPRTFDHLVEQAKAEQAAADAPEQDELLEKARTLALEHSR
                     ISTSMLQRRLRIGYPRAARLIDTLEDEGIVGEGESSASREVIGSERDSVFGDL"
     gene            5105..6943
                     /locus_tag="VIM45_00185"
     CDS             5105..6943
                     /locus_tag="VIM45_00185"
                     /inference="COORDINATES: protein motif:HMM:NF013225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEY8819737.1"
                     /translation="MKNLAEFLAARRFHDSEQPVLPEAGNCINCGADLAESQLYREYR
                     ICERCRYHYSVGAHRRVELLVDPASFRESNRSLVSVDPLSFRAQAQYRRRFFQEQRRT
                     GLSDAIVTGTGSIAGRRIVLAALDFRFLGGSVGNAVGEKLARALENGARNKLPVVMIV
                     ASGGVRMQEGLPALMQFAKVAEAAGRLAGSGEPLITVLATPSLGGSYAVLGGLADLRI
                     AEPGALVGYATTRMVEERAGRPAAEGARTAEAHLARGLIDEVVDRARLRDFISSLLEM
                     LASRPQQGGAAAGRAMQFAPATHSAWTTIQLARHAERPTASDYIGHFSDTFVELRGDD
                     VTGDDRAIICGIGMLGAEPVVYVATERARNGAPKTVAGPAGFRKARRAYELAGRLRLP
                     IVTLIDRVEASPAIEAEEAGLGPALADCMSALGMVATPAIGAIIGEARGEAALALGIV
                     DKMLMLENAVFEVVSPETAASIIYRDTGMADTVATSLRPTARDCKELGIVDIIVPEPA
                     AGAHTEHEAAARLLGAALLRSLGELRGVAPRKLVRSRYNRYRRIGQYTNFLSATVGHD
                     VAQLSGDIARRASGAIARLTHRGRRTPGTRGPSISDDSDGAGLAVP"
     gene            complement(6951..9053)
                     /gene="uvrB"
                     /locus_tag="VIM45_00190"
     CDS             complement(6951..9053)
                     /gene="uvrB"
                     /locus_tag="VIM45_00190"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HEY8819738.1"
                     /translation="MPPFKLRSDFKPTGDQPDAIEMLIEGVEKGYRQQTLLGVTGSGK
                     TFTMANVVERLQRPTLVLAHNKTLAAQLCSEFKEFFPENEVEFFVSYYDYYQPEAYIP
                     RTDTYIAKDADINDEIDKLRHAATRALFERRDVLIVASVSCIYGLGEPEEYKSFVLNI
                     EKGGRMHRNEAIRGLVNMQYERNDINLVRGKFRLRGDTLTIHPSYDELAVRVDFWGDT
                     VERIIELDPLTGEVLAEKDHVDIYPAKHFVTSQEKLDAAMADIEQELHDRLAEFRSEG
                     RILEAARLEERTRYDLETLREAGYCSGVENYSRHLARRAAGSTPWTLLDYMPADWLLF
                     VDESHMSLPQVRGMFHGDYARKRTLVDFGFRLPSAIDNRPLNFEEFDRHINQVIYVSA
                     TPGPYERDVSAQTVEQVIRPTGLLDPTIEIKPTKGQIDDLMDEIQRRVDRRERVLVTT
                     LTKKMAEDLTDYLQEMGIKTNYLHSEVNTLDRVEILRDLRLGVYDVIVGINLLREGLD
                     LPEVSLVAILDADKEGYLRSEGSLIQTMGRAARHERGHVIMYADTITGSMQRAIDETT
                     RRRQIQIAYNQEHGISPESIRKAIRDITDHVRKVAEEKPEYRAGSGMPRDTVARLVID
                     LESQMKQAAKSLEFEKAALIRDQIVDLRRELLGDEEGLQRIAEMIVPRRRDEGRLVTP
                     ESTRYSNRAVRGRSRRGP"
     gene            complement(9157..9756)
                     /gene="ruvA"
                     /locus_tag="VIM45_00195"
     CDS             complement(9157..9756)
                     /gene="ruvA"
                     /locus_tag="VIM45_00195"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEY8819739.1"
                     /translation="MSYIARLRGIVEEKGRDNVVVDVGGVGYEVNVPGSTLARIGAAG
                     DQVTLFTYMYVREEALQLYGFLTPEEKDTFARLLGVTGIGPRSALSFLTQFSPGELSA
                     VVERRDATALTRVKGIGRRTADRLILELQGKLNPSGADIPAFPATGGSIDPALDVLVS
                     LGYSAAEAGMALARVSLPGQVGDEERVTQALRALDILRG"
     gene            complement(9813..10349)
                     /gene="ruvC"
                     /locus_tag="VIM45_00200"
     CDS             complement(9813..10349)
                     /gene="ruvC"
                     /locus_tag="VIM45_00200"
                     /inference="COORDINATES: protein motif:HMM:NF000711.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEY8819740.1"
                     /translation="MSTPANGLPDMNGEVVILGVDPGLRITGYGAVVCTPARTQALDY
                     GHIKTDSRAAIEARLHTIYWGIRAAIRRFNAQELAIELPFVAVNIRSAFAIGEARAAA
                     MLAAAEEHITVFQYTPAEVKQCVSGYGRSDKHQIQENVRLQLGLPSLPTPADAADALA
                     IAICHYANRRMRALERMH"
     gene            complement(10360..11109)
                     /locus_tag="VIM45_00205"
     CDS             complement(10360..11109)
                     /locus_tag="VIM45_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015407589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY8819741.1"
                     /translation="MSGHSKWSQIKRQKGVADVRRGAVFTKLGREIVVAAKQGGGDPT
                     ANFRLRLAVQRAREANMPADTIDRAIKRAAGGGEGAELEEIAYEGYGPGGIAIMVEAM
                     TDNRNRTAASIRSTFDRGGGNLGETGSVAWQFVARGVVRVDARGKDSDDIELAAIDAG
                     AEDVRSEDSEVIVYTERGSVEAVRRGLTDAGYEVTASDLHLVPENTVELDEAKSIQAL
                     KLLDKLEADDDVQNVYTNADFAESAMEAYNA"
     gene            11312..12205
                     /locus_tag="VIM45_00210"
     CDS             11312..12205
                     /locus_tag="VIM45_00210"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEY8819742.1"
                     /translation="MAAPQVKPIEQRGYVHPEVLVSTDYVAQHLEDRGIRILEVNEDI
                     LLYDTGHIPNAVKIDWQQHLNDPVRRDFIGSERFAKLMDEIGVGNDTTVVFYGDRNNW
                     WACYAYWIFQLNGHDKVKVMDGGRKKWIDEGRELTKEVPRYSSTGYKAQRPKPGIRAF
                     RDDVLGHIGGRQPKPNADKPLVDVRSPQEYSGELMHMPDYPQEGTLRGGHIPGAKSIP
                     WARAVDADTATFKSADELKQIYGDQGVTPDKDVIAYCRIGERSSHTWFVLHELLGYPK
                     VRNYDGSWTEWGNAVALPIER"
     gene            complement(12286..13143)
                     /gene="panC"
                     /locus_tag="VIM45_00215"
     CDS             complement(12286..13143)
                     /gene="panC"
                     /locus_tag="VIM45_00215"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEY8819743.1"
                     /translation="MIVEPRIDGVRAWRRSLSGSVGVVPTMGYLHEGHISLVDRARAE
                     NDHVAVTIFVNPTQFGPREDLASYPRDLDGDLRLLEQTGVDLVFTPEAAEVYPPDFDT
                     WVDPGGIANRLEGSSRPGHFRGVATVVLKLLNIIAPDRAYFGQKDAQQLRVIKQMVRD
                     LNVPVNVIGLPIVREADGLAMSSRNAYLSDRERQAALVLSQALRRVAEALHSGVTDAR
                     QLRGLAEALINAQPLASLEYVSVADGETLDELEAADTGALVSLAVRIGKTRLIDNVSL
                     GASVTAVAR"
     gene            complement(13140..13997)
                     /gene="panB"
                     /locus_tag="VIM45_00220"
     CDS             complement(13140..13997)
                     /gene="panB"
                     /locus_tag="VIM45_00220"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041223361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEY8819744.1"
                     /translation="MKERGEKIPMLTAYDYPTAKLLEAAGVPIMLVGDSLGMVVLGYD
                     STLPVTMEDMIHHGKAVVRGTERAIIVVDMPFMSYQISTEDALRNAGRLLKETGATAV
                     KLEGGEPVAGTVRRMVDAGIPVMGHLGLTPQSVNQLGGYRVQGKTPAAAVKLINDAAA
                     LERAGAFAIVLETIPAYVAKMVTDKIGVPTIGIGAGPYCDGQVQVVHDFLGLFPDFLP
                     RHARRFADLAPTIDGAVKAYIDQVKACEFPTAKESFGLDKARKALEGGECVTPYGGGS
                     VREMEASKA"
     gene            complement(14011..14943)
                     /locus_tag="VIM45_00225"
     CDS             complement(14011..14943)
                     /locus_tag="VIM45_00225"
                     /inference="COORDINATES: protein motif:HMM:NF022188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rossmann-like and DUF2520 domain-containing
                     protein"
                     /protein_id="HEY8819745.1"
                     /translation="MTPILANPPLLSESEELLPSTPSIGLIGLGAVGAALSLALAAAG
                     NHVAAVYSRNLNSSQRISEQLESATVTGSAQEVVDLCDVVLLTVSDGSIETLAASLDW
                     RPGQIAVHCSGVLGTVALAAARRQGATAVALHPLCTLPREGGAPIPTGTRFAFTGPGE
                     ARGFFTSLVAGLGGVLIDVPEEARPLYHAAAVMAGNYTVALTAVAVSLLGSLGLTRQE
                     GLAALVPLLHGTVDNLERSGLPDALTGPIVRGDLGTVAAHRRALRDTEFLELYDVLAR
                     ATAGLALQREELSNEVRQTLLGLKWGCNCDSTFD"
     gene            complement(15096..16112)
                     /locus_tag="VIM45_00230"
     CDS             complement(15096..16112)
                     /locus_tag="VIM45_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HEY8819746.1"
                     /translation="MRKSAQRRGPALSGRDSFADVPGLRVGHWTNSRAATGCTVILAP
                     PDGVAAAVAVRGGAPGTRETDVLRPGNLVQRIHAILLTGGSAFGLNAAGGVMRYLEEQ
                     NIGFETRHGRVPIVVGAVLFDLGIGSAKVRPGEADGYKACLSAVRRVREGSVGAGTGA
                     TVAKAGGIDRAIKGGIGTASERLHDGTVVAALAAVNAYGDIRDPETGALIAVPRPDPT
                     GGVETAVELLRRRGSRATSEFTSTTLVVVATTATLARDQLIRVAEMAHGGLARAIEPS
                     HSPGDGDIVFAISTGNETGSENKPDLLAIGALGARAVARSIVRAVECATSLAGVPVVS
                     DGHA"
     gene            complement(16196..17050)
                     /locus_tag="VIM45_00235"
     CDS             complement(16196..17050)
                     /locus_tag="VIM45_00235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8819747.1"
                     /translation="MTQRVGHDIATAVCEECGRPVADPSPDAGGRGCVRCADRYLRSL
                     DAEFLDNYARFGARGRLVVAEACLRQLVISDVADRKLLAMTVYEQFVAAATDFLGLYH
                     ALLNRRAVPIVKGVLGFELDVAAAMVFFNDLASKGPAEILYAFGLPQPEQVRVLAAGL
                     DTRERGQVAAALAEALADLDRVLAFQVVGEQALVSAARRLGGPMALADQTNWLCGTRL
                     IPGQIATLALNRERSGVEINLLNTDEMTLGAVVDGIDTMTRLTRNVIFAFVSLHGPEQ
                     FEHGFRDG"
     gene            complement(17047..17889)
                     /gene="mutM"
                     /locus_tag="VIM45_00240"
     CDS             complement(17047..17889)
                     /gene="mutM"
                     /locus_tag="VIM45_00240"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HEY8819748.1"
                     /translation="MPELPEVETVRRGLEPAVVGRTIRDVRFTPEGERLLQGVASHQF
                     RDYLVGRRFEAVSRRGKYLIFPLDDGRCFVAHLRMTGRFEVERATAAEGKYFRAAILL
                     DDGNELRWRDARRFGTWQISEDASEIDRKLGPEPLEDTFTPDALGAAARGRKAPIKSV
                     LLDQRRVAGLGNIYVDEALHDAGIHPSRRAGTLTTEELQTLHGAITRVLRSGISNFGT
                     TMRDFVNAYGQEGRNREHLRVYQRRGEPCYRCGTAIERIVIGGRGTHYCPHCQPAPPE
                     ALKA"
     gene            complement(17908..>20508)
                     /gene="polA"
                     /locus_tag="VIM45_00245"
     CDS             complement(17908..>20508)
                     /gene="polA"
                     /locus_tag="VIM45_00245"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEY8819749.1"
                     /translation="VLKNLQPTHIAVTMDTEAPTFRHEKEATYKAHRPDMPDDLRGQF
                     ARVRDVIEAFNIPIYEADGFEADDVLGTLARQAELLGVETYLVTLDSDILQLVRDDSV
                     RCYMFRLYQHDTVTYDEAKVFERYGVRPDQIPDLKGLKGDTSDNIPGVPGIGEKTAVK
                     LVQQFGSVRGVLDRIDEVTPPKLQELMRTYGERALHCREMATIVTDAPVELSLDYCRA
                     ADFDRGKVMDLFHALEFRSLLDRVPSGTLAAGAAAEPEAAADDREAVYRTVTSIEQLD
                     TVIAAIRESGQLAFDTETTSTNAYNAKIVGISLAANPFEAFYIPVGHFPSLEGPDQLD
                     VSTVVDRLRPVFDDPAIEKVAHNAKYDMIVLNNAGLPVERIDSDTMIAAYLLGDRDIG
                     LKSLAREILGVKMTPISDLIGTGAKQITMAQVPIETASQYASADADLTLRLRPSIEEE
                     LRSRGLFDLYKEIELPLVSVLMHMEKTGIAVDVEVLRNMSRTLTGDLLRIEAEIYESV
                     GHQFNLGSPKQLSQVLFEELKLPSGRKTTQGFTTDAQTLEALRGNHPVVEQILEYRQL
                     FKLKSTYIDALPGMIDLNDGRLHTNFSQTTAATGRLSSSDPNLQNIPVRTELGKQVRR
                     AFVARDPVNGRPMSLLAADYSQIELRILAHVTQEPRLVAAFKADEDIHKTTAADIFGV
                     PVADVTPDQRRLAKTVNFAVIYGLSAAGLSSRTELSMGEAGQFIDAYFKRYPGIKQYL
                     QDTIAHTRQLGFAETLLGRRRYLPDINTGNFNLRQAAERMATNHPIQGTNADIIKIAM
                     NRIEEELRARSLDSRMILQVHDELIFECPDSELDIIKELVPRWMCSAMTLSIPVKVEL
                     KTGKNWGEME"
BASE COUNT         3792 a         6494 c         6311 g         3911 t
ORIGIN      
        1 cgtactattt gaattgccga tcgtgatgat ggtgccggca aaattcggca ttatccgcgc
       61 cagaaaatac ctgaaatggt ggcgatacgc gattgtcctt ggtttcctgg ctgctgcggc
      121 cgttatcccg aacattagtc cgatcgaaca gatatcagtc gcgattccta taattggcct
      181 gtattttgta ggcgtggggc tggcaaagct cgtacagccg aaacaagcag ccgtttctga
      241 ataatctctc tggtaatctt caacttggcc tgcatcaacc gttcattgtc cgcaatcgct
      301 ggcccgtgct agcatggagc cactatgcag accaatctcg gcgagcttag ccagctaatc
      361 aagcgctgcc ccgattgcga cctttcccag acccgtacgc aggccgtccc cggagaagga
      421 ccggacaccg ccgctatcat gttcatcggc gaagggccag gctattacga agacaagagc
      481 ggacgcccct ttgttgggaa cgccggcaaa ttccttgacg aattgctgca gcatgctggt
      541 ctaaagcgct gcgatgtttt cattacgaac gtggtaaagt gccgcccgcc ccagaaccgt
      601 gacccgcttc gcgatgagat cgacgcgtgc gacaagtggc tcaagcatca gatcaacgtc
      661 atcgatccga aagtcatcgt gacactcggg cgcatttcaa tgggtaaatt cttcgcgggc
      721 gaatccatca gcaagattca cggccaaccg cacaaagtag gcgaccgaac gatcgtgcca
      781 atgttccatc cggctgccgc gctccaccaa gaacgattcc gatcgctcat cgtcgatgat
      841 ttcaagcagt taccggaagt actggagtcg gtccagcggg agaaggctgc ctcagaccag
      901 ccggcacagc cttcaaccga gcagggccgt ctcttctaac aagagattaa agaggtacca
      961 tggcagacca ccagactctg cgcgtcattc cgctcggcgg ccttggcgaa atcggcaaga
     1021 acatgatggt gttcgagctt gccgatgaca tcgtcgccgt cgattgcggc ctccagtttc
     1081 ccgaagccga aatgctaggc gtggacctgg ttattccgga taccacgtat cttcgggagc
     1141 gactcgacaa agtccgcgcg atcctgatta cgcatggtca cgaggaccat atcggcgcgt
     1201 tgccctacgt gcttcgcgat gtcaacgtgc ccgtgtactg cacacggctc acatatggcc
     1261 tcatctcggt gaagttgaag gagcaccgac tgctgaggga gagcgatctg cggatcgtcg
     1321 agcccggcga acagattcag ttgggcgact tcactgcgga gtttttccga gtagcgcaca
     1381 gcattcccga tgcttgcggc atcatcttgc acactccgct cggccgcgtc gttcacacgg
     1441 gcgacttcaa gctcgaccat acccccgtca tggggcagca caccgacctg ccgcgcctca
     1501 cggaactggg taatgaaggc gtgacgctcc tctgcgccga ctcgacatat gcagaaatcc
     1561 ccgggtacac accgtccgag caggtggtcg gcgtcgcgct ggacaacatc atgaaccagg
     1621 ccgaggggcg cgtcatcgtg gcaacctttg ccagcctgat ctcgcgcgtc cagcaggtaa
     1681 tcgacgcggc agttcatcac aaccggcggg tatttgtgat cggccgcagc atggtcgata
     1741 acgttaacat ggcgctcgaa cagggctacc tcgaggtgcc agacggcgtt ctcgggcgta
     1801 tcgaagagat gcgtaacctg ccggtacacc gcgtcgcgat cgtcacgacc ggcagccagg
     1861 gcgaaccaac ttcagccctc tcgcggatgg cgaatcgcga ccatcgctgg gtgcagatcg
     1921 ttcccggaga tacggtcgtg ctctcggcat cgccgattcc cggcaacgaa cagtttgtct
     1981 acaacgtggt cgataacctc ttcaagctcg gtgctaaagt gctttataac cgcgtggaca
     2041 acatccacgt ccgcggacac gccgcacagg aagagttgaa gctgctcttg agcctcgtgc
     2101 ggcctaaata cctgcttccg atccacggcg aataccggca cctgatgctt cacgcccgca
     2161 tcggccaggc gatgggcatg gcgcctgagg atacgttcgt catcgaggac ggtgatgtac
     2221 tcgagattac ccaggacggt gccgaggtgg cgggacgggc acccgccgcc gcggtgtacg
     2281 tcgacggcct cggcatcggc gatgtcgatc acgtcgtctt acgcgaccgc cggcacctcg
     2341 ctaacgacgg tatggtcgtc gtcgtcgtcg ctgtcgacaa acagacgggt aaagtcatcg
     2401 atctgccgga tgtgctttcg cgtggctttg tagaagccag cgaggcaggg gaactcatgg
     2461 atcgggtgcg agatgtcgcg atgcaatcgc ttgatggcgc cgatcaccgt gcggagtggc
     2521 cagatatcaa cgctaaagtg aaagacacgg tcgccaagtt cctctatgat gaaacccggc
     2581 ggcgcccaat ggtgctgcct gtagccgtcg aggtctaggt cgggcgggac ccgggctctc
     2641 gcaatcgaat actggagccg taccccatgg cgaaagcgaa ggggtcttcc cgcccgcgta
     2701 cctcgctgtc atctccgcgt gccgagcgca gacgcaaacc cgcgtttcgt ggcgacattg
     2761 ccgagatggc gctgaggccg atcaccccgg tcgtcgcgtt tctcctcact ccagccgggc
     2821 ttgcaacagc catcgttttg atcgtggcgg cagccatgcc gtggctatcg attgtcgacg
     2881 tgcttgacgc ctttcggcgg gcggtggtcc aggggaccgg cgtcgtctgc tatctcatcg
     2941 tgatcgacct gctggcgctg gtggcgattc cgggcattgc ccccggtccg cgaacctcac
     3001 gccgcttctg gagaatcgcg accggcttcc agttcctcgg actgtttgtg tggggtgtcc
     3061 tcggcgccct gcaccccgaa acggcgctgg caggcgtgtc gcttcaagac tattcggccg
     3121 gcggcaacgt tggtcatagc ctcgtggcca gcgggttcgg cgttctggtc tggctcctcg
     3181 cgctctccat cggctgcgcc ctggtcagcc cgtcgggtac gcggtgggcg ctcgcgcaac
     3241 tgccccctgc ggcgcggtcg ctatgggcac tcaacatccc gggcaaactg ctcgccgcgc
     3301 ttggcaccct gcgaccggcg cgcactccct acgccgttgc cggcacctcg gcggtctcgc
     3361 tgccggacat cgaggaatcg ccgccacccg aaacgattgg ccagggcgct gccgatgcct
     3421 tccgccggac cggcgagcga gaacgtcaac ggcaggcgct cgcgccgacg cttgaacttc
     3481 ccgggttcgg aacgggtacc acagccgcac cgcatggcgt cggctggcaa ctgccaaaca
     3541 cacaactgct cagcacgtcc gccccaagcc agctcagccc tggggacaac gaagcgcgcg
     3601 cgcggacgat cgtcgaaacg ctcgccagct tcggcgtaga cgcaaaggtc ctgcagatca
     3661 accagggccc gtcggtgacg cagttcggcg tggaaccggg ctgggagatt aagacacgaa
     3721 acgatcctga gcgcgacgcg agcggcaaag tgcttttaga caaagacaac aagccccgca
     3781 tgcgcgtgga ggaggtttcc cggacgcgtg ttcgcgtgaa ccagatccaa aagcttcaga
     3841 acgacctggc gcttgcactc gccgccccga cgattcgcat cgaggcgccg gttccgggca
     3901 agccgatggt cggcatcgaa gttccgaaca cgagctcgtc ggtcgtcagc ctgcgaagcg
     3961 tcgtcgaaac gccgcagttc cagaaggttg cgtcgaagac caaactggcg ctggcactcg
     4021 gaatgggcgt ttctggagag cctattatcg ccgacctggc tacgatgccc cacctactca
     4081 tcgcggggac gacagggagc ggcaagagcg tctgtatccg cgccctgatc tcgtgcatcc
     4141 tcatgcacgc aacgccggac gacgttcgcg ttgtacttat cgacccgaag cgcgtcgaga
     4201 tggctgtatt cgcgccgatc ccgcacctgg cactctccaa gatcgtcacc gacatggagg
     4261 aggtggctgg gaccttgcag ggcgtgatcc gggagatgga ggaccgctat aagaagttcg
     4321 aaatgttcaa ggtgcgcaac ctggaagcgt acaaccgcaa cccgaaagtc gccggcaagc
     4381 ttccaaactg ggtcgtcatc atcgacgagc ttgccgacct gatgatggca gtgccatacg
     4441 aggttgaacg gcagatctgc cggctcgcac agcttgcacg tgctaccgga attcatctag
     4501 tcgtggcgac gcagcggccg tcggtggacg tcattaccgg cctgatcaag gccaacttcc
     4561 caacccgtat tgcgttcgcg gtttcgtcgc aggtagattc gcgtgtcatc ctggacggcg
     4621 tcggcgccga gaagctgctc ggtcgcggcg acatgctttt catgcccaca gaccaggcaa
     4681 agcggcgcgt gcagggggtg tacgtctccg accaggagat cgagcggctg gtcgacttct
     4741 ggggcgccga gcgctgggaa aagcttcgtc cgcggacatt cgatcacctc gtcgagcagg
     4801 caaaggccga acaggcagcc gccgacgcac ccgaacaaga cgagctattg gaaaaggcgc
     4861 gcacactcgc gctggaacac tcgcgaatct cgacatccat gctgcagcgg cgcctccgca
     4921 tcggctaccc acgagctgcc cggctcatag acacgcttga ggacgagggc atcgttggcg
     4981 agggcgagag ttcagcctca cgcgaggtga taggatccga gagggatagc gttttcgggg
     5041 acctgtagtc gggaataggt cgggcgggta tgcggtggtc cacctgctct gccagcggag
     5101 gcgtatgaag aacctggccg agttcttggc cgcgcggcgc tttcacgata gcgagcaacc
     5161 ggtgctgccg gaggcgggca actgcatcaa ctgcggcgcc gacctcgcgg agtcccagct
     5221 ctatcgcgag tatcggatct gtgagcgctg ccgctatcac tacagcgttg gagcccacag
     5281 gcgcgtcgaa ctgctcgtcg atcctgccag ctttcgcgag agcaatcgat cgctcgtctc
     5341 tgtcgacccg ctttcgttcc gtgcgcaggc gcagtaccgg cgccgcttct tccaggagca
     5401 gcggcgcacg ggcctgagtg acgccatcgt aaccgggact ggatccatcg ccggccgccg
     5461 catcgtactg gctgcgctcg acttcaggtt cctgggaggc agtgttggca acgcggtcgg
     5521 cgagaagctt gctcgcgcgc tcgaaaacgg tgcgcggaac aaacttcccg tcgtcatgat
     5581 cgttgccagc ggcggagtcc ggatgcagga agggctgccg gcgctcatgc agttcgccaa
     5641 agtggcggag gctgccggtc gccttgccgg ttcgggcgaa ccgctcatta cggtgctggc
     5701 gacgccaagc cttggcggca gctacgcggt cctgggcggg cttgcagacc tgcgcatcgc
     5761 cgaaccagga gccctggtcg gatacgccac gacgcggatg gttgaagagc gtgccggccg
     5821 cccggcggca gaaggcgccc gcaccgcgga agcgcatctc gcgcgtgggc taatcgacga
     5881 ggtggtagac cgggcccgtt tacgcgactt catctcttcc ttgttggaga tgctcgcgag
     5941 ccgtcctcag caggggggcg cggcggctgg acgagctatg cagttcgctc cggccacgca
     6001 tagcgcctgg acgaccatcc agctagcccg ccacgccgag cgccccaccg cttcggatta
     6061 catcggccat tttagcgaca cttttgtcga gctgcggggc gatgacgtca ccggggacga
     6121 ccgggcaatc atctgcggca tcgggatgct tggcgcagaa cccgtcgtct acgtcgccac
     6181 cgaacgggcg agaaatggcg cgccaaaaac cgtcgccgga cccgcaggat tccgaaaagc
     6241 gcgtcgcgcc tatgagctcg cggggcgatt gcgtctgccg atcgtcacac taattgacag
     6301 agtcgaagca tcgcctgcca tcgaagccga agaagcgggc ctcggtccgg ccctcgccga
     6361 ctgcatgtcg gcgttgggca tggttgcgac tcccgccatc ggcgcaatca tcggcgaggc
     6421 ccgtggcgaa gcagcgctgg ctcttggcat agtcgacaag atgctgatgc ttgagaacgc
     6481 cgttttcgag gttgtttcgc cagagaccgc cgcctcgatc atctatcgcg acaccggaat
     6541 ggctgacacg gttgcgacat cgcttcgccc aacggctcgc gactgcaaag agctggggat
     6601 cgtcgacatc attgtgcccg agccggcggc cggcgcccac acagagcacg aagctgccgc
     6661 tcggctgcta ggcgccgcgc tgctcagatc gctaggcgaa ctacgcgggg tcgctccacg
     6721 taagctcgtg cgatcacgct acaaccgcta ccggcggatt ggccagtaca cgaacttcct
     6781 cagcgccact gtcggccacg acgtggccca actgagcggc gatatcgccc gcagagccag
     6841 cggtgcaatc gcacgcctca cgcaccgcgg ccggcgcacg cccgggacca gaggaccgtc
     6901 aatttccgac gacagcgacg gtgccggcct cgcggtaccg tagcgcgctc ctacggccct
     6961 cgacggctcc tgccgcgcac cgcccgattc gagtagcgcg tcgattctgg ggttaccaga
     7021 cggccttcgt cgcgacgacg tggcacaatc atttccgcga tccgttgcag accttcttcg
     7081 tcacccagca gttcgcggcg cagatcgacg atctggtccc ggatcaaagc agctttctca
     7141 aattcgagtg acttcgccgc ctgcttcatc tgggactcga gatcgattac caggcgggct
     7201 accgtatctc tcggcattcc actgcccgcc cgatactctg gcttctcctc ggcgactttg
     7261 cggacgtggt cggtgatgtc cctgatcgcc ttgcggatgc tttccggcga gatgccgtgc
     7321 tcctgattat aagcgatctg gatctggcgg cggcgcgttg tttcgtcgat cgcgcgctgc
     7381 atcgaaccag tgatggtatc ggcatacatg atcacatgtc cgcgctcgtg tcgcgccgct
     7441 cgccccatcg tttggatcaa tgatccctcc gaacgcaggt acccttcttt gtcggcgtcc
     7501 aggatcgcga cgaggctcac ttcgggcagg tccagtcctt cgcgcagcag gttgatgccg
     7561 acgatgacgt cgtagacgcc cagacgcagg tcccgcagga tctcgacgcg gtcgagcgtg
     7621 ttcacctccg aatgcaggta attggtcttg attcccatct cctgcagata gtcagtgagg
     7681 tcctcggcca tcttcttcgt cagggtcgta accagcacac gctcccggcg atcgacgcgc
     7741 cgttggatct cgtccatcag gtcgtcgatc tgacctttcg tcggcttgat ttcgatcgtc
     7801 gggtccagca gaccggtagg ccggatgacc tgctcgaccg tttgcgcgga cacgtctcgc
     7861 tcatacggtc ccggcgtagc ggagacgtat atcacctggt tgatgtgacg atcgaactcc
     7921 tcaaaattga gcgggcgatt gtcgatcgcc gacggtagcc ggaagccgaa gtcgaccagc
     7981 gtgcgcttgc gggcatagtc gccgtgaaac atgccgcgca cctgcggcag gctcatgtga
     8041 gactcatcca cgaacagcag ccagtcggcg ggcatgtaat cgagcaacgt ccacggcgtg
     8101 ctgccggcgg cccggcgagc gaggtgccgg ctatagttct cgacgcccga gcagtaaccg
     8161 gcctcccgca acgtttcgag gtcgtagcgc gtgcgctcct ccagccgagc cgcctcgagg
     8221 atccgcccct cgctacgaaa ttccgccagc cgatcgtgca gctcctgctc gatgtcggcc
     8281 atggcggcgt ccaacttttc ctgggaggtg acgaaatgct tggcggggta gatgtcgacg
     8341 tggtccttct ccgccaacac ctcaccggtc aacgggtcga gctcgatgat ccgctcaacc
     8401 gtgtcacccc agaaatcgac gcgaacggca agctcgtcat atgacgggtg gatcgtgagg
     8461 gtgtctcccc gcaaacggaa ctttccgcgt acgaggttga tgtcattgcg ctcatattgc
     8521 atgttgacga ggccgcgaat ggcctcgttg cgatgcatcc gcccgccctt ttcgatattg
     8581 agcacaaaac tcttgtactc ttccggctcg cccagaccgt aaatacacga aacggatgcg
     8641 acgatcagca cgtcgcgtcg ctcgaacagg gcgcgcgtcg cggcgtggcg cagcttgtcg
     8701 atctcgtcgt ttatgtcggc gtctttggcg atgtacgtgt cagtgcgagg aatatacgct
     8761 tccggttgat agtagtcgta ataggagacg aagaactcga cttcgttctc tggaaagaac
     8821 tctttgaatt cgctgcacag ttgggcggcg agcgttttgt tgtgcgctag cacgagtgtt
     8881 ggccgctgca gccgttcgac gacgttggcc atggtgaacg tcttgcccga gccggttact
     8941 cccagcagcg tctgctgcct gtagcccttc tcgacgcctt cgatcagcat ttcgatggca
     9001 tcaggctgat cgccggtcgg cttaaagtcg gagcggagct tgaacggcgg catgagcgcc
     9061 tcgcagtctt acggcggtac attggttaga acgtatgacc tattatacca gtttcgatgg
     9121 cagccggcag tcgccaccag cgggggcttt ggttgctcaa ccgcgcagta tgtcgagggc
     9181 gcggagtgcc tgggtaacac gttcttcgtc gccgacctgg cccggtaacg agacgcgggc
     9241 gagggccatc cctgcttcgg cggcgctgta gccgagcgag acgagaacgt ccagtgccgg
     9301 gtcgatggag ccgccggtcg cagggaatgc tggtatatct gcacccgaag ggtttagctt
     9361 tccttgaagc tccagaataa gccggtcggc ggttcttcga ccgatgcctt taacgcgcgt
     9421 gagggccgtc gcatcccgtc gctcgacgac cgcactcagt tcgcctggag agaactgcgt
     9481 caggaacgac agagcgctgc gcggcccgat gccggttacg ccaaggagac gggcaaaggt
     9541 gtctttctct tcgggcgtca gaaagccgta gagctgaagc gcctcctcgc gcacgtacat
     9601 gtaggtgaac agcgtcacct gatcgcccgc ggcaccgatt cgcgccagcg tgctgcccgg
     9661 gacgtttact tcgtagccca cgccaccaac gtctacgacg acgttgtcgc ggcccttctc
     9721 ttcaacaatg ccgcgcagac gcgcgatgta gctcattgcg caggaactcc ctttgggcga
     9781 gcgtagcctc gtctaaagac cgtgacccgg agtcagtgca tacgttccag ggcgcgcatg
     9841 cgccggttcg cgtaatgaca gatcgcgatc gcaagcgcgt cggcggcgtc cgcgggcgtg
     9901 gggagcgacg gcagcccaag ctgcaagcgc acgttctcct gaatctgatg tttgtcgctg
     9961 cgtccgtacc cggagacgca ctgcttcact tccgccggcg tgtactgaaa gacggtgatg
    10021 tgttcctctg ccgcggccag catagcggcc gcgcgggcct ccccgatcgc gaacgctgac
    10081 cgaatgttga cggctacaaa aggaagctca attgccagct cttgcgcgtt gaagcgtcgg
    10141 atggcagccc gaatacccca ataaatcgtg tgcagccgcg cctctatggc cgcgcgcgaa
    10201 tcggtcttaa tgtggccgta atccagagct tgcgtgcgag ccggcgtgca caccacggca
    10261 ccgtagccgg tgatgcggag accggggtcg acaccaagaa taacgacctc cccgttcatg
    10321 tcgggaaggc cgttggcagg tgtactcacg tccgaccgct caggcgttgt aggcctccat
    10381 cgcagactcg gcgaaatcgg cattcgtgta gacgttctgg acgtcgtcgt cggcctctaa
    10441 cttatcgagc agctttagcg cctggatgct ctttgcctcg tcgagctcaa cggtgttttc
    10501 agggaccaaa tgaaggtcgg atgcggttac ttcgtagccg gcgtcagtga gcccgcgtcg
    10561 cactgcttca acgcttccgc gttcggtgta cacgatcact tcgctatctt ccgaccgcac
    10621 gtcctcggcg ccggcatcga tcgccgcaag ctcaatatcg tcggaatcct tgcccctggc
    10681 gtcgaccctg accacaccac gcgcgacgaa ctgccacgca accgagccgg tttcaccgag
    10741 gttgcctccg ccccggtcga acgtcgaacg gatgctcgcc gccgtacggt tgcggttgtc
    10801 ggtcatggcc tcgaccatga tcgcgatccc gccggggccg tagccctcgt acgcgatctc
    10861 ctcgagctca gctccctcac cgccgccggc ggcgcgcttg attgcgcgat cgatcgtgtc
    10921 cgcgggcatg ttcgcttcac gcgcgcgctg gacggccagg cgcaagcgaa aatttgcggt
    10981 cgggtcgcca ccgccctgct tcgcggctac gacgatctcg cggccaagct tggtgaagac
    11041 cgcgccgcga cgaacgtctg cgacaccctt ttgccgcttg atctgtgacc acttggagtg
    11101 accggacact tctcgccttc gtttccggat tcagtcagtc gattttacca ccgccgtcga
    11161 aagggggtca aaatgccgtg gcatatcttg cccactggtc cgctggccgc aagcggcctg
    11221 cgcgatgcgc cgctgagcgg tgttagactg atatgtcggc acacgcgatt cttggcagac
    11281 agcatgagta aggcgaagaa aggaaacatc catggcggcg ccacaggtca agcccatcga
    11341 gcagcgcggc tatgtccatc cggaagttct ggtctccacc gactacgtgg cccagcattt
    11401 ggaggaccgt ggcattcgga tcctggaggt caacgaagat atcctcctct acgacaccgg
    11461 ccatatccca aacgcggtca agatcgactg gcagcagcac ttgaacgacc ctgtccgccg
    11521 cgactttatc ggctcggagc gctttgccaa gctcatggac gagatcggcg tcggcaacga
    11581 cacgaccgtc gttttctacg gcgaccgcaa caactggtgg gcctgctacg cctactggat
    11641 cttccaactc aacggccacg acaaggtcaa ggttatggac ggcggtcgca agaagtggat
    11701 cgacgagggc cgcgaactga ccaaagaagt gccgcgctac tcaagcaccg gctacaaggc
    11761 gcagcggccc aagccgggga tccgtgcctt ccgcgacgac gtcctcgggc acatcggcgg
    11821 acggcagccc aagccgaacg ccgacaagcc gctggtagac gtgcgctcac ctcaggagta
    11881 cagcggtgag ctgatgcaca tgccggatta cccgcaggag ggaacactgc gcggcggcca
    11941 catccccggc gccaagagca tcccctgggc gcgggcggtg gacgccgaca ctgcgacctt
    12001 caaatcggca gacgagctga agcagatcta cggtgatcag ggtgtgacgc ctgataagga
    12061 cgttatcgcc tattgccgca tcggtgagcg atcatcgcat acctggttcg tgctgcacga
    12121 gcttctgggc tacccgaagg tgcgcaacta cgacggttcc tggaccgagt ggggcaacgc
    12181 cgtagcgctg ccgatcgaac gctgacctga gccgaggagt cgggataccg tgcgcgtatt
    12241 ccgaccgccc ggtcggagaa acgtcctgtg tcgaatcctt tgtggttatc tggcaacagc
    12301 cgtaacgctt gcgcccaagg acacgttgtc gatcagccgc gttttgccga tgcggaccgc
    12361 aagactgacc agagcgccgg tgtccgcggc ttccaactcg tccagcgttt cgccgtcggc
    12421 aacgctgacg tattccagtg acgccagggg ctgagcgttt atcaacgctt cggctaggcc
    12481 ccgaagttgg cgggcgtcgg taacacctga gtgcaatgcc tccgctacac gccgaagggc
    12541 ctgagacagc accagagccg cctggcgttc gcggtcgctc aggtaggcgt tacggctgct
    12601 catcgccagc ccgtcggcct cacggacgat gggcagcccg atgacgttca ccggcacgtt
    12661 caggtcgcgc accatctgtt tgataacgcg aagctgctgt gcgtcctttt gaccaaagta
    12721 ggcgcggtcc ggggcgatga tgttgagcag cttcaacacg accgtggcga cgccacggaa
    12781 gtggcccgga cgcgaggacc cctctagccg gttggcaatt cctcccgggt cgacccaggt
    12841 gtcgaagtcc ggaggatata cctccgcggc ctcgggcgtg aacaccaggt caacgcccgt
    12901 ctgctcgagc aatcgcaggt ctccgtccag gtcacgcgga tagctggcga gatcctcccg
    12961 cgggccgaac tgggtcgggt tgacaaagat cgtcaccgcc acatggtcgt tctcggcgcg
    13021 ggcacggtcc accaggctga tgtggccctc gtgcaggtag cccatcgtcg gtacaacgcc
    13081 gaccgatcct gagagcgaac gccgccaggc acgaacgccg tcgatgcggg gctcgacgat
    13141 cacgccttgc tggcctccat ctcccgaacg gagcccccgc cgtacggcgt cacgcactcg
    13201 ccgccttcaa gcgccttgcg ggccttatcg agaccgaagc tctctttggc ggtcgggaac
    13261 tcgcaggcct tcacctggtc gatgtatgcc ttcacggcgc catcgatggt tggggcgagg
    13321 tccgcgaacc ggcgcgcgtg tcgtggaaga aagtccggaa acaaccccaa gaagtcgtgc
    13381 acgacctgga cctggccgtc gcaatagggg ccggcgccga ttccgatcgt cggtacgcca
    13441 atcttgtccg tcaccatctt ggcgacatat gcagggatag tttcgagcac gattgcaaac
    13501 gcgcccgcgc gctcgagggc ggcagcgtcg ttgatcagct tgactgccgc ggccggcgtc
    13561 ttgccctgaa ctcggtagcc accaagttga ttgacagatt gcggcgtgag tccaaggtgg
    13621 cccatcacgg gaataccggc atcaaccatg cgccgcaccg tgccggcgac cggttcgccc
    13681 ccctcgagct tgaccgccgt cgcccccgtt tctttcaaca ggcggccggc gttgcggagc
    13741 gcgtcctccg tcgagatctg gtagctcatg aatggcatgt caacgacgat gatcgcgcgc
    13801 tccgtgcccc gcacgaccgc tttcccatgg tggatcatgt cctccatggt caccggcaac
    13861 gtggagtcgt agccgagcac aaccataccc agcgagtcgc cgaccagcat gatcggcacg
    13921 ccggctgcct cgagcagttt ggcagtcggg tagtcatacg cggtaagcat gggaatcttc
    13981 tccccacgct ccttcattgc ctttagttca ttaatcgaaa gtcgagtcac agttgcaccc
    14041 ccatttcaac cccaacaacg tctgtcggac ttcattgctc aattcctcgc gttgaagcgc
    14101 caacccggcg gtcgccctcg caagaacgtc gtacagttct aaaaactcgg tgtcccgaag
    14161 ggcgcggcga tgtgctgcca ccgtgccgag gtcgccccga acgatcgggc cggtgagcgc
    14221 atccggcaga cccgaccgtt ccaggttgtc cacggtgccg tggaggagcg gcaccaatgc
    14281 ggccaagcct tcttgtcgag ttagtcccag agaaccgagg agtgagaccg caactgccgt
    14341 aagcgccacc gtgtaattac ctgccatcac cgccgcggcg tggtagagcg gtcgcgcctc
    14401 ctcgggtaca tcgatgagca ctccccccag ccctgcaacg agcgatgtga agaacccccg
    14461 cgcttcgcct ggtcccgtaa aagcgaaccg ggttccagtc ggaatgggcg cgccgccctc
    14521 acgaggaagc gtgcagagcg gatgcaacgc gacggccgtc gctccctgcc gccgggcagc
    14581 ggcgagcgcg actgtgccga gcaccccgct gcagtgaacg gctatttgcc ccggtcgcca
    14641 gtccaatgag gcggccagcg tttcgatgga cccgtctgag acagtcaaaa gaacgacgtc
    14701 gcacagatcg acgacctcct gggccgagcc agtaacggtt gccgattcta actgttcgga
    14761 gatgcgctgg gacgagttga ggttacgtga gtaaaccgct gcgacatgat tgccggcggc
    14821 agcgagcgcc agagaaagtg ccgcaccgac cgctcccagg ccaatcaggc caattgaggg
    14881 cgtcgacggt aagagttcct ctgattcgga cagaagaggc gggttcgcga gtattggcgt
    14941 catctgggcc ccgtctcggt cacataggat ccaagcggtt cgttggtcgg actacccgtt
    15001 gcttgctacc ggcgccgctc accgccacaa acgtggtcgg ctgctcgttc aggataccag
    15061 cgtggcaaag cgtgtcaaga atgaattttg acgagttagg cgtggccatc cgagaccacc
    15121 ggcacgcctg ccaggcttgt cgcacactct acggcacgaa cgatactgcg cgccacggcg
    15181 cgggcgccaa gggcgccgat ggcgagcagg tcaggcttat tctccgatcc cgtttcgttc
    15241 ccggtagaaa tcgcgaagac gatgtcaccg tcgccgggcg agtgcgacgg ttcgatcgcg
    15301 cgtgcgagac cgccgtgcgc catctccgcc acacggatca actggtctcg ggcgagcgtt
    15361 gccgtcgtcg ccaccaccac gagcgtcgtg ctcgtgaact cggacgtggc gcggctgccg
    15421 cgtcgtcgta agagctccac cgccgtttca acgcctccgg tcgggtcggg tcggggcacc
    15481 gcgatcagcg cgccggtttc tggatcgcga atgtcgccgt acgcatttac ggcggccagc
    15541 gcggcgacga ccgtaccgtc gtgcagccgt tccgatgctg tgcctattcc ccccttgatc
    15601 gcgcggtcga tgcctccggc cttagcgacg gttgcgcccg ttccggcacc tacacttccc
    15661 tcgcgaaccc tcctgactgc cgaaaggcag gccttatacc catcagcttc tccgggcctg
    15721 accttcgcgc tcccgatacc caggtcgaac agaaccgcgc caaccacgat cggcacgcgg
    15781 ccgtggcggg tctcaaaacc gatgttctgt tcctcgaggt agcgcatgac gccgccggcc
    15841 gcgttcagtc cgaaggcgct gccgccggtc agcagaattg cgtggatgcg ttgaacgagg
    15901 ttgccggggc gaagcacgtc agtctcacgg gtgccgggtg cgccgcctcg gaccgcgacc
    15961 gcggcagcaa cgccgtcggg aggggccaga atcaccgtac aaccggtggc tgcccgcgaa
    16021 ttggtccagt gtccgacccg caggccgggc acgtcggcaa agctgtcgcg gcccgataac
    16081 gcgggtccgc gccgctgcgc gctttttcgc attgcatatt gctcgcgcga tttcgggctg
    16141 aaagttccgg tcgcccgacc agcatacgcc ctcacgttgc agcccggcga cctgctcagc
    16201 cgtcgcgaaa gccgtgctcg aactgctcgg gcccgtgcag cgacacgaaa gcaaaaatca
    16261 cgttgcgcgt caggcgagtc atcgtgtcga tcccgtcgac gacggcgccg agcgtcatct
    16321 catcagtgtt gagcagattg atttcgacgc cggagcgctc gcggttcagc gccagcgttg
    16381 cgatctggcc ggggatcagc cgcgttccgc atagccagtt cgtctggtcg gcgagcgcca
    16441 tcggaccgcc gagccggcgc gctgcgctca ccagagcctg ttcgccgaca acctggaagg
    16501 ctagcacccg atcgaggtcg gcgagcgcct ccgccaacgc cgcggcgacc tgtccgcgct
    16561 cgcgcgtgtc caatcccgca gcaagcactc gcacctgctc tggttgcggc agcccgaatg
    16621 cgtagagaat ttcggccgga cccttactcg cgaggtcgtt aaagaagacc atcgccgcgg
    16681 ccacgtcaag ctcgaacccg agcacgcctt tgacaattgg gacggcacgg cgattcaaca
    16741 gcgcatgata caagccgaga aagtccgtag ccgccgccac gaactgctca tacaccgtca
    16801 tcgccaggag cttgcgatcg gcgacatcgc tgataaccag ttggcgcagg caagcctcgg
    16861 cgacgacaag ccggccgcgg gcgccaaagc gcgcgtagtt gtcgagaaat tcggcatcca
    16921 ggctgcgcaa atatcgatcg gcgcaccgca cacaaccgcg gccgccggca tccggcgatg
    16981 gatcggcgac gggccgcccg cactcttcgc agaccgccgt cgcgatgtcg tgaccgaccc
    17041 tttgcgtcat gctttcaatg cctctggagg cgccggttga cagtgtggac agtaatgggt
    17101 accgcggcca ccgatgacga tgcgctcaat cgctgtgcca cagcgatagc atggctcgcc
    17161 acgccgttgg tagacgcgta gatgctcacg gttgcgaccc tcctggccat acgcgttcac
    17221 aaaatcacgc atcgtcgtac cgaagttact gatgccgctt ctgagcactc gggttatggc
    17281 gccgtgcaat gtctgaagct cttctgttgt gagcgtcccc gcgcgtcggc tcggatgaat
    17341 gccggcgtcg tggagcgcct cgtccacata gatattaccc aggccggcta ctcgccgttg
    17401 gtccagcaat accgacttga tcggagcctt tcgtcccctg gccgcggctc ccaacgcatc
    17461 tggcgtgaac gtatcttcaa gcggttccgg ccccagtttg cggtcgattt cgctcgcatc
    17521 ttcggagatc tgccaagtgc caaagcggcg cgcatcgcgc cagcggagct cgttgccgtc
    17581 gtcgaggaga atcgccgcgc ggaaatattt tccttcggcg gcagtggcgc gctcgacctc
    17641 gaatcgcccg gtcatgcgaa ggtgagccac gaagcaccgg ccgtcgtcga gcggaaagat
    17701 gaggtacttg ccacgccggc ttactgcttc gaaccgccta ccaaccaggt aatcgcggaa
    17761 ctggtgcgac gccacgccct gcaacagccg ctcgccctcc ggcgtaaagc gcacgtcacg
    17821 aatcgtacga ccgacgacgg cgggctcgag gccgcgccgg accgtttcga cttctgggag
    17881 ttctggcatg gttacctgcc gagcccgcta ctccatttcg ccccagtttt tgccggtctt
    17941 aagctcgact ttgacgggga tagagagcgt catggcggag cacatccagc gaggaaccag
    18001 ctccttgatt atgtcgagtt ccgagtcggg acattcgaag atcagctcgt cgtgaacctg
    18061 gaggatcatt cgcgaatcga gcgatcgcgc acgaagctcc tcttcgattc ggttcatggc
    18121 gatcttgatg atgtccgcgt tcgtgccctg gatcgggtga tttgttgcca tgcgctcggc
    18181 agcctgacgc aggttgaagt ttcccgtatt gatgtccggc aaataccgcc gccggccgag
    18241 cagcgtttcg gcaaagccta gctgccgcgt gtgggcgatc gtgtcctgca ggtactgctt
    18301 tattcccggg taacgcttga aatatgcgtc gatgaactga ccagcttctc ccatcgataa
    18361 ctcggtgcga gacgagagac cggcggctga caaaccatag ataaccgcaa aatttaccgt
    18421 cttggccagc cgtcgctgat cgggcgtgac gtctgccacc ggcactccga aaatgtccgc
    18481 ggcagtggtt ttgtggatgt cctcgtctgc cttgaacgcc gcgacaagcc ggggctcttg
    18541 cgtgacgtgc gcgaggatcc gcaactcgat ttgcgagtag tcggcggcga gcagtgacat
    18601 tggtcgcccg ttcaccgggt cgcgcgccac gaacgcccgc ctgacctgct tgccaagctc
    18661 cgtgcggacg gggatgttct ggaggtttgg gtccgacgac gaaagtcgac cggtggccgc
    18721 ggtcgtctgg ctgaaattgg tatggagccg gccgtcgttc aggtcaatca tgcccggcaa
    18781 ggcgtcgatg tacgtcgact tcagcttgaa aagctggcgg tactcgagga tctgttcgac
    18841 cacgggatga ttgccgcgca gcgcctcgag cgtttgggcg tcggtcgtaa agccttgagt
    18901 cgtcttccgg ccgctaggca acttcaactc ttcgaacaac acctggctta gctgctttgg
    18961 agagcccagg ttgaactggt gtccgacgga ttcatagatt tccgcctcga tgcgaagcag
    19021 atctccggtc agcgtgcgcg acatgttgcg gagcacttcg acgtccactg cgatgccggt
    19081 cttctccata tgcatcagga cgctgaccag cggcagctct atctccttgt acaggtcgaa
    19141 gagcccgcgc gagcgtaact cctcttcgat gctcggacgc agacgcagcg tcagatcggc
    19201 atcggcggag gcgtactgcg acgcggtctc gatcggaacc tgcgccatgg tgatctgctt
    19261 ggcgccggtg ccgatgagat cgctgatcgg cgtcatcttg acgccgagga tttcgcgcgc
    19321 cagcgatttg agtccgatgt cgcggtcgcc gagcaggtag gccgcgatca tcgtgtcgga
    19381 gtcgatgcgc tcaaccggaa gcccggcgtt gttgagcacg atcatgtcgt acttggcgtt
    19441 gtgcgcgacc ttctcaatcg ccggatcgtc gaataccggg cgtaaccggt caaccaccgt
    19501 cgagacgtcg agttggtcgg gaccttccag gctgggaaag tgtccgaccg ggatatagaa
    19561 cgcttcaaac ggattcgccg cgagcgatat accgacgatt ttggcgttgt aggcgttcgt
    19621 gcttgtcgtt tccgtgtcga atgccagttg accgctctct cgaattgcgg cgatcacggt
    19681 atcaagctgc tcgatggagg taaccgtgcg gtacacggct tcgcgatcat cggccgctgc
    19741 ctccggctcc gcggcagcac ctgcggccag cgtgcctgat ggcacgcggt caagcaacga
    19801 gcggaactcc agcgcgtgga agaggtccat cacctttccg cggtcgaagt cggcggcgcg
    19861 gcagtaatca agcgaaagct cgacgggcgc atcggtgacg atggtcgcca tctcgcggca
    19921 gtgcagcgcc cgctcgccgt aggtgcgcat caattcctgc agcttcggcg gcgtcacctc
    19981 gtcaatgcga tcgagaacgc cgcgaacgct gccaaactgc tgaacgagct ttacggccgt
    20041 cttttcgccg attcccggta cccccggtat attgtccgac gtgtcgcctt tcagtccctt
    20101 gaggtcgggg atctggtccg ggcggacccc gtatcgctcg aacaccttgg cttcgtcgta
    20161 ggtaaccgtg tcgtgctggt aaagccggaa catgtaacag cgaacgctgt cgtcgcgcac
    20221 gagttgcaag atgtcgctgt ccagggtgac gaggtaagtt tcgacaccca aaagctcggc
    20281 ctggcgtgcg agagtgccca gcacgtcgtc ggcctcgaag ccgtctgcct catagatggg
    20341 gatgttgaac gcctcaatga cgtctctcac gcgggcgaac tgtccgcgga ggtcgtcggg
    20401 catatccgga cggtgcgctt tatacgtcgc ttccttctcg tggcggaagg tcggtgcctc
    20461 agtgtccatg gtgacggcga tgtgggtggg ttgcaggttc ttcaacac
//
LOCUS       DATCPE010000008         4791 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U7219
            k141_454488, whole genome shotgun sequence.
ACCESSION   DATCPE010000008 DATCPE010000000
VERSION     DATCPE010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955033
            Sequence Read Archive: SRR1586279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 4791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 16:49:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 640
            CDSs (total)                      :: 631
            Genes (coding)                    :: 627
            CDSs (with protein)               :: 627
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4791
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_454488"
                     /isolate="SMAG_U7219"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            98..547
                     /locus_tag="VIM45_00250"
     CDS             98..547
                     /locus_tag="VIM45_00250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8819750.1"
                     /translation="MTRRPGSRSASIERPRQKGWWGEPESDQIADDFEFEPPAPRVVM
                     RKPGTSHPERSVTQVGRALTPGQIDSIRAVMRLFAQDDTANGIDPHSSLMCHRCNAAR
                     RAIGSIRYDRFTLCNECSIDYEILHARGLVDGVVEYLERFASRAASA"
     gene            complement(751..1251)
                     /locus_tag="VIM45_00255"
     CDS             complement(751..1251)
                     /locus_tag="VIM45_00255"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY8819751.1"
                     /translation="MARYSKAPSMTIDLTKKYIATFHTEKGDFKVELFAKDAPMTVNN
                     FVTLARDGFYDNVTFHRVIPGFMAQGGDPTGSGSGGPGYTFVDEQNTRKHEGGTLSMA
                     NAGPNTNGSQFFITFEPQPHLNGRHTVFGQVTDGMDVVRKLRVRDPGSDRTPGDRITS
                     IDIGEE"
     gene            complement(1292..2146)
                     /locus_tag="VIM45_00260"
     CDS             complement(1292..2146)
                     /locus_tag="VIM45_00260"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY8819752.1"
                     /translation="MAEGSRTLSNRSPSRKRRDRRYDPLKPIGGFRHEPKSFFDRLVH
                     HKWAFPVISVVGGAALILGTILLFLNPNGGSTGRAVRPGEDAVRGGTAPAVIETPGAT
                     PAGTPQPTGTPAPQRAYNAAPAFTIDPTAKYSAVIKTDMGDITIALDARSAPQTVNNF
                     VFLAQHRFYDGLTFQRVVKNFVAQAGATSADGSGGPGYTIPDENSPLKHDPGMVAMAQ
                     TGTSTNSAGSQFYVALAPLPQQDDKETVFGQVTSGLDILQSLSARDPRDPNAGPALAI
                     QSISIQKQ"
     gene            complement(2260..2334)
                     /locus_tag="VIM45_00265"
     tRNA            complement(2260..2334)
                     /locus_tag="VIM45_00265"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2299..2301),aa:Arg,seq:ccg)
     gene            2467..3918
                     /locus_tag="VIM45_00270"
     CDS             2467..3918
                     /locus_tag="VIM45_00270"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HEY8819753.1"
                     /translation="MLAERRLIIASNRGPVEFARNPDGSLSANRGSGGLVTALSALSR
                     FSDLTWIASAMTDGDREMAAKAEGSVPFDESGMHIRFVPLPRKLYQQYYNVISNSHLW
                     FLQHYMWNTPRSPNIGRAVYEAWEQGYARANETFAEAIAAEASGDRTPPYVMVQDYHL
                     YLVPKLVRERLPGTIIQHFTHIPWPDPRYWALLPNFMRRAIFENLSSADIVGLQTVRD
                     TRNFLQGAEAFLPGADVDYRRSTVWFNGHLTLVHAYPVAVDAEGLLAFARSEKVREYE
                     RRLEPLFVQKSVVRVDRAEPSKNLLRGFRAFELLIERHAEYRSRVNMLAFIVPSRTDI
                     GFYQNYTDEVLELIDAINDEYGSATWEPITVFYENNYAQAIAAMRHYDVLLVNPVIDG
                     MNLVAKEGPLVNVNDGVLLLSETAGAFEQLAEHALPVAPADIEGTVRALKRALDMPAE
                     ERHTRALALHDVIAADDIIEWLIRQFSDLLALA"
     gene            complement(3893..4741)
                     /gene="otsB"
                     /locus_tag="VIM45_00275"
     CDS             complement(3893..4741)
                     /gene="otsB"
                     /locus_tag="VIM45_00275"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HEY8819754.1"
                     /translation="MKSATERGGAAETETGASRLRRVLHSRPLGLFVDTDGTISPLAA
                     TPQSAVVSPEAKALLRQLSTRIRVVVISGRTLVDLRRMVGLSGVTYVGSHGLATWIDG
                     REELDDSVRPYVGYARQAMAELASLRRIDGLLFEEKGTGIGIHYRLARDRDEARSAIL
                     RGVAASGSAAHFDLLEGIRVIELRPRLGINKGTSVRALVNRFRLEGLIYVGDDITDVE
                     AFEAIRDTQSGGRIDGLAIAVRHGEASPLVEAAADYAVDGVEGVVSLLGFIARSVGFM
                     PAPADR"
BASE COUNT          892 a         1497 c         1502 g          900 t
ORIGIN      
        1 gccaatccgg ctggataccg gaggcatacg tttcccctcc ctgacctggt gctccgacgt
       61 cgtcatcgcc gctaagcgtg aataggaggc agcgacagtg acacgccgtc cagggagtcg
      121 gtcagcatca atcgagcgcc ccaggcagaa gggctggtgg ggcgagcccg aatccgatca
      181 aatcgccgac gacttcgaat tcgagccacc ggcgccgcgt gtggtcatgc ggaagcccgg
      241 gacctcgcat ccggagcggt cggtaacaca agtaggacgg gcgctcacgc ccggtcaaat
      301 tgattcgatc cgcgccgtca tgcgcctgtt cgcgcaggat gacacggcga atgggatcga
      361 tccgcacagc agcctcatgt gccaccgctg caacgcggca cgtcgggcaa ttggcagtat
      421 ccgctacgac cgttttacgc tctgtaacga gtgcagcatc gattacgaaa tcctccacgc
      481 cagggggcta gtggacggcg tggtcgaata cctggagcgt ttcgcatcga gggcggcttc
      541 cgcgtagggc cgttcggtgg cagtaggcag tcggcagtcg tattgagcat tgccccgttt
      601 cggggatggg ccggggggtt cgtcccgcgc tgcaagccag ctcgaaccca tctaccaacg
      661 gtcgcccctt cccgtcgtta gggaaggggc cagggggtta ggtctcgccc gactgccaac
      721 tgccgggctg cgcgccccgc ggcgcagccg ctactcttcg ccgatgtcga tgctggtaat
      781 gcggtcaccg ggggtgcggt cggaaccggg gtcgcgaact cgaagcttgc gaacgacgtc
      841 catgccgtcg gtgacctggc cgaatacggt atggcggccg ttcaagtgcg gttggggctc
      901 gaacgtaatg aagaactggc tcccgttggt gttggggccg gcgttcgcca tcgataacgt
      961 gccgccctcg tgcttacgcg tgttttgttc atcgacaaac gtatatcccg ggccgccgct
     1021 gccggatccc gtcgggtcac cgccctgggc catgaaccct ggaatgaccc gatggaaggt
     1081 cacgttgtcg tagaaaccgt ctcgggccag agtcacgaag ttgtttaccg tcatcggcgc
     1141 gtccttcgcg aagagttcca ctttgaaatc ccctttttca gtgtggaacg tagcgatgta
     1201 cttcttcgta aggtcgatcg tcatcgacgg tgctttgctg taccgtgcca tgtttgcctc
     1261 tgttctgtgt attccggctt acgccgctgt actactgttt ctggattgaa attgactgga
     1321 tcgccagcgc gggacccgcg ttcggatcgc ggggatcgcg cgcggataac gactgtaaga
     1381 tatcaagtcc cgaggtcacc tgcccgaaaa ccgtctcctt gtcgtcctgc tgcggcagag
     1441 gcgcgagtgc gacgtagaac tgactgcccg ccgaattcgt cgacgtgccg gtctgtgcca
     1501 tcgccaccat tccgggatcg tgcttgagcg gactgttctc atcgggaatc gtataccccg
     1561 gcccgcctga gccatcggcg ctcgtggcgc cggcctgcgc gacgaaattc ttaacgacgc
     1621 gctggaacgt caaaccgtcg tagaagcgat gctgggccag gaaaacgaag ttgttgacag
     1681 tttgcggcgc tgagcgggcg tcgagtgcga tggtgatatc gcccatgtct gtcttgatga
     1741 ccgccgaata ctttgccgtc ggatcgatcg taaaggcggg cgccgcgtta tatgcgcgtt
     1801 gtggggccgg ggtaccggtc ggctgcggtg tgccggccgg tgttgcgccc ggggtttcga
     1861 tcaccgcggg ggccgtaccg ccgcggaccg cgtcttctcc cggacggact gcgcgtccgg
     1921 tgctgccgcc gtttggattc aagaacagta ggatggtgcc cagaatgagc gccgcaccac
     1981 ccaccacgga aatgaccgga aacgcccact tgtggtgaac gagccggtca aaaaagctct
     2041 tgggctcgtg tcgaaagccg ccgatcggtt taagtggatc gtatcttcgg tcgcgccgct
     2101 tacggcttgg tgacctgttg ctcagagtcc tgctcccttc ggccacgcgg ggtcgccgct
     2161 tgacccggtt tatgggctgc gccgacagcg ctcgatgctc cggcccacgc ggtcaagaca
     2221 acggccgggc cgcatggccc ggcccgtata ggtcccgcgt ggtacccccg acaggactcg
     2281 aacctgtgct tccggctccg gaggccggcg ctctatccac tgagctacgg gggctcagtg
     2341 ccgtaagtgt agcgcgggca cattcgcagc ggcaaggcag acggccgttg cgcgacgcaa
     2401 ggcgtattgt tgagcagggg agaaaacaaa ttgcccatcg acgcgctgcg ggaactttca
     2461 tcccaaatgc tggccgaacg acgcctgatc atcgcgagca atcgcgggcc ggtggagttc
     2521 gcccggaatc cggacggatc gctgagcgcc aatcgcggca gcggaggtct ggtgacggcg
     2581 ctttcggctt tgagtcgctt ctccgatctg acctggattg cgagcgctat gaccgacgga
     2641 gaccgagaga tggccgcaaa agcagaaggc tcggtgcctt tcgacgaaag cggcatgcac
     2701 atccgctttg tcccgctgcc gcgcaagcta taccagcagt actacaacgt gataagtaac
     2761 tctcacctct ggttcctgca gcactacatg tggaacacgc cgcgctcgcc caacatcggt
     2821 cgcgccgtct atgaagcatg ggaacagggt tacgcacgcg caaacgagac gttcgccgag
     2881 gcgatagcgg ccgaggctag cggcgaccgc acgccaccgt atgtcatggt gcaggactac
     2941 cacctgtacc tcgttccgaa gctggtgcgt gagcgcctgc ccggaacgat catccagcac
     3001 ttcacccaca ttccctggcc cgacccgcgg tattgggcgc tgctgccaaa cttcatgcgt
     3061 cgggcaatct tcgagaacct gagctcagcc gacatcgtcg gactgcaaac cgtgcgcgac
     3121 acccgcaatt tcctgcaagg cgcggaggcg ttcttgcccg gcgcggacgt ggactaccgc
     3181 cgctccaccg tgtggttcaa cggccacctg acgctggttc acgcctatcc ggtcgccgtc
     3241 gacgctgaag ggctgctcgc cttcgccagg tccgaaaagg tacgcgaata cgagcggcgg
     3301 ctcgagccgc tctttgttca aaaatcggtt gtccgtgtcg accgcgcgga gccgagcaag
     3361 aacttgctcc gcggcttccg agcttttgag ctgttgatcg agcgacacgc tgagtatcgt
     3421 agccgcgtca atatgctcgc atttatcgtg ccgtctcgaa cggacatcgg cttctaccag
     3481 aactacacgg atgaagttct cgagctcatc gacgcaatca acgacgaata cggctcagcc
     3541 acgtgggagc cgatcaccgt gttctacgaa aacaactacg cgcaggccat cgcggcgatg
     3601 cgccattacg acgtgctgct cgtgaacccg gtgatcgacg gtatgaacct tgtcgccaag
     3661 gagggtccgc tggtcaacgt gaacgacggc gttctcctct tatccgagac cgccggtgcg
     3721 tttgagcaac tggcagaaca cgccctaccg gtggcgcccg cggacatcga agggaccgtc
     3781 agagcgctca aacgtgcgct cgacatgccg gccgaggaac gtcacacacg ggcactggca
     3841 ttacacgatg tgatcgccgc cgacgacatc attgaatggc tgatccgcca gttcagcgat
     3901 ctgctggcgc tggcataaac cccacagacc gcgcaataaa gccgagaaga ctgacgacgc
     3961 cttcgacgcc gtcgaccgcg tagtcggccg ccgcctcgac gagcggtgag gcttcaccgt
     4021 gccgcactgc aattgccaaa ccgtcgatcc gaccaccgga ctgcgtgtct cggatcgcct
     4081 cgaacgcctc cacgtcggtg atgtcgtcgc ccacgtagat caatccttcg agacggaagc
     4141 ggttcactag agcgcgaacc gaggtgccct tgtttatgcc gagcctgggt cgcagctcga
     4201 tgacgcggat accctctaag aggtcgaagt gcgcggcaga accggacgcg gcgactccgc
     4261 ggagaatagc cgaacgggct tcgtcgcgat cgcgcgcgag ccggtagtgg atgccaatac
     4321 cggttccctt ctcctcgaag agaagcccgt cgattcgccg caacgaagcc agctcagcca
     4381 tcgcttgtcg cgcatagccg acgtatggcc tgaccgaatc gtcaagctct tcccgcccgt
     4441 cgatccacgt ggcgagccca tgcgagccga cataggtcac ccccgagagg cccaccatgc
     4501 ggcgaaggtc gaccagggtg cgaccagaaa taaccacgac gcggatgcga gtcgagagct
     4561 ggcggagcag cgcctttgct tcgggtgaga ccacggcgga ctgcggcgtt gccgcgagcg
     4621 ggcttatggt tccatcggtg tcgacgaaca gccccagcgg acgagaatgc aagacgcgac
     4681 gcagtcgtga tgcgccggtt tcggtttcgg cggcgcctcc gcgctccgtg gcagacttca
     4741 tcaggtcgag ccacgcgttc ggggatcgag cgcgtctcgg agtccgtcac c
//
LOCUS       DATCPE010000009         3545 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U7219 k141_763,
            whole genome shotgun sequence.
ACCESSION   DATCPE010000009 DATCPE010000000
VERSION     DATCPE010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955033
            Sequence Read Archive: SRR1586279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 16:49:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 640
            CDSs (total)                      :: 631
            Genes (coding)                    :: 627
            CDSs (with protein)               :: 627
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3545
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_763"
                     /isolate="SMAG_U7219"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..755)
                     /locus_tag="VIM45_00280"
     CDS             complement(<1..755)
                     /locus_tag="VIM45_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8819755.1"
                     /translation="MTNSRFPMVPVLFALVAACSIALGSAPGPARADNALTVNGTPTV
                     DNKFPTEVQFSIDATSSAGDINDATLHYQLLPSNVGVSARAEFDTGKIVHAVYHLRTA
                     GNPLYVPPTKTIRYYWTLQDASGNQTKTDPVDWSYDDTRFKFKKASNGNLTLYYYTGS
                     DANAQRILAVGRGALDKASALNGAPLDFPIHLVTYGNQAEAGAAFSHESKSFDPSILG
                     QADPPDIVVLVAGDLSGAENEDTVRHELTHLVNA"
     gene            complement(795..1860)
                     /gene="prfB"
                     /locus_tag="VIM45_00285"
     CDS             complement(join(795..1847,1849..1860))
                     /gene="prfB"
                     /locus_tag="VIM45_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_148311191.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEY8819756.1"
                     /translation="MVRLDIATREKQAAALETETAQPDFWQDAQSAQQVMRRLSDLRG
                     SIEPWTLVRRRVADLLELIELSLAEGDESIVADLAADADASKREVEELEFELMLGGEY
                     DNNAAILAVHAGAGGTESQDWAEMLMRMFLRWAERRRFAVEVLSVTPGEEAGIKSATL
                     EITGRHTYGYLRSERGVHRLVRLSPFDSAHARHTSFALVEVMPVVEEATAEIDINPDD
                     LRVDVFNASGHGGQNVQKNATAIRITHLPTGKVVTCQNERSQHRNRDSAMKVLRSWLM
                     EQELERRADERARLKGEHVAAGWGNQIRSYVLHPYKLVKDHRTGYETTDPAAVLEGDL
                     DPLMRAYLQWDIGNSGGAAA"
     gene            complement(1902..2609)
                     /gene="nfi"
                     /locus_tag="VIM45_00290"
     CDS             complement(1902..2609)
                     /gene="nfi"
                     /locus_tag="VIM45_00290"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010936345.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HEY8819757.1"
                     /translation="MKLNPTHSWDVSPADARGIQERLRWRVSAEGGPAPEDVRFVAGT
                     DISVGNKERPGRAAVVVIEYPSLEPIEQSVVEGRPAFPYVPGLLSFREIPLLAPAFER
                     LESTPDLVVVDGHGVAHPRRFGLASHLGMLLGVPTIGCAKSRLVGSFDEPADETDAVS
                     PLYDGEQLIGQVVRTRKGMRPIFVSVGDHIGLKQATEWVLRLTRGFRLPEPTRLADQA
                     AAGRTIVDRKEGQPWKS"
     gene            complement(2613..>3545)
                     /locus_tag="VIM45_00295"
     CDS             complement(2613..>3545)
                     /locus_tag="VIM45_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydrofolate dehydrogenase/cyclohydrolase
                     catalytic domain-containing protein"
                     /protein_id="HEY8819758.1"
                     /translation="ADAIVDRRRAVERCMTAEVINGHEVAAQFRAELASRIAVLRRAG
                     VTPGLAVVLVGNNAASVSYVRGKTRAAAELEILSESVRPDPDISQADLLNLVNQLNAD
                     DRFHGIIVQLPLPDGIDERAITAAVDPRKDVDGLHPINLGRLLRGDGRLLPCTPAGVR
                     ELLVRSGHGPDGRHVVICGRSNLVGKPLAAILLQKQTGANATITVCHTGTPDLGAVTR
                     LGDILVVATGRANMITADMVRPGAVVVDVGVNRVSDASRKSGYRLVGDVDFESVSRVA
                     RAITPVPGGVGPMTVTMLLVNTVLAAEASSLRYT"
BASE COUNT          598 a         1143 c         1118 g          686 t
ORIGIN      
        1 gcgttgacga ggtgcgtcag ctcgtggcgt accgtgtctt cgttctccgc gccgctgagg
       61 tctccagcca cgagcacgac gatgtccggc gggtcggcct ggccaaggat cgagggatcg
      121 aacgatttgg attcgtgaga aaacgccgcg ccggcttctg cctggttgcc ataggtaacg
      181 aggtggatcg ggaaatcgag cggcgcaccg ttgagtgcgc tcgctttgtc gagcgctccg
      241 cgaccaaccg ccagtatgcg ctgggcattg gcgtcgctgc cggtgtagta gtacagcgtc
      301 aggttgccgt tcgacgcctt tttgaacttg aagcgcgtgt cgtcgtagct ccagtcgacc
      361 gggtctgtct tggtctggtt tcccgacgca tcctgcagcg tccagtagta gcgaatcgtc
      421 ttggtcggcg gaacgtagag cgggttcccg gccgtgcgca ggtgatagac cgcgtgcacg
      481 atcttgccgg tgtcaaattc ggcgcgagcg ctgacgccga cgttgctggg cagtaactgg
      541 tagtgcagcg ttgcgtcgtt aatgtcgccc gcactggacg tggcgtcgat gctgaactgc
      601 acttcggtcg ggaacttgtt atcgacggtc ggcgtcccgt tgaccgtgag tgcgttgtcg
      661 gcgcgagcag gtcccggggc cgagcccagt gcaatcgagc aggcggcgac gagcgcgaat
      721 agcaccggca ccattgggaa ccgcgaatta gtcacgccac ttgcctccgc ctcaaccgcc
      781 ctgcacccgc cctatcaagc cgctgcgccg ccgctgttgc cgatgtccca ctgtaggtac
      841 gctcgcatga gcggatcgag atcgccctcg agtactgccg ccgggtccgt cgtctcatat
      901 ccggttcgat ggtccttcac cagcttatac gggtggagga cgtagctacg aatctgatta
      961 ccccaacccg cggcgacatg ctcgcctttc agtcgcgccc gctcgtcggc ccgacgctcc
     1021 aattcctgct ccatgagcca ggatcggagc accttcatcg ctgagtcacg gttgcggtgc
     1081 tgcgatcgct cgttctggca ggtgacgacc ttgccggtcg gcagatgcgt aatccggatc
     1141 gcggtcgcat tcttctggac gttttgacca ccgtgtccgc tggcgttgaa gacgtcgacg
     1201 cgcaggtcgt cggggttgat gtcgatctcg gcggtcgcct cttcgaccac cggcatgacc
     1261 tcgaccagcg cgaacgacgt gtgacgcgcg tgcgccgagt cgaacggcga cagccgcacc
     1321 aggcgatgca cgccgcgttc cgaccgcagg tagccgtacg tgtgccgccc ggtgatttcg
     1381 agcgtcgcac ttttgatgcc ggcctcctca ccgggagtaa cgctcaatac ctctaccgcg
     1441 aagcggcgcc gctctgccca gcggagaaac atccgcatta gcatctccgc ccagtcctga
     1501 gattcggtgc cgccggcgcc cgcgtgcacg gccagaatgg ccgcgttgtt gtcgtactcg
     1561 ccgcccaaca tcagttcgaa ctcgagctct tctacctcgc gcttcgaggc gtcggcgtcc
     1621 gcggcaaggt cggcgacgat gctttcgtcg ccttcggcca gcgagagctc gataagttcc
     1681 aacaggtctg cgacgcgccg tcgaaccagt gtccacggct cgattgagcc gcgaaggtcg
     1741 gagagcctgc gcattacctg ttgcgcggac tgagcgtctt gccagaaatc aggctgtgcc
     1801 gtctccgttt cgagcgcggc ggcctgcttt tctcgtgtcg caatgtcaaa gtcgcaccat
     1861 gattgtatcg atacggctta gaagctcctg aagctcttct tttagctctt ccacggttgt
     1921 ccttccttac gatcgacaat cgttcgtccg gcggcggcct ggtcggccag tcgtgtcggc
     1981 tccggcagcc gaaatccgcg ggtcaatcgc agcacccact cggttgcctg tttcaggccg
     2041 atgtgatcgc cgacggaaac gaatatcggc ctcatgcctt tgcgcgtgcg cacgacctgt
     2101 ccgatgagct gttcgccgtc gtacaacggg ctgacggcgt cggtctcgtc ggcgggctca
     2161 tcgaagctgc cgacaaggcg ggatttagcg cagccaatgg tcggaacacc gagcaacatg
     2221 ccgaggtggg atgccaggcc aaatcgccgc ggatgcgcca cgccgtggcc atcgacgacc
     2281 accaggtccg gcgtcgattc cagccgctcg aacgccggag caagcaacgg gatctcgcgg
     2341 aacgacagca gaccgggaac gtaagggaac gcaggccgtc cctccaccac tgactgctcg
     2401 atcggctcta gagacgggta ctcgatcacc accacggccg ctcgcccggg ccgctccttg
     2461 ttaccgaccg atatgtcggt gccggcgacg aagcgcacgt cttcgggtgc aggcccgcct
     2521 tcggctgaaa cccgccaccg taggcgttcc tggatgccgc gtgcgtcggc gggggaaaca
     2581 tcccagctat gcgtcgggtt gagtttcaca cgtcaagtat agcggaggga ggaggcttcg
     2641 gcggcgagga ccgtattgac gagcagcatc gtcaccgtca tcgggcccac gccgcccggt
     2701 acgggcgtga ttgcccgagc aacgcgcgat acgctttcaa agtcgacgtc gccgaccaac
     2761 cggtagccgc tcttccggct cgcgtctgag acgcggttga cgccaacgtc cacgacgacc
     2821 gcgcccggac gcaccatgtc cgcggtgatc atgttcgccc gccccgttgc gacgacgagg
     2881 atatcgccca gtcgggtgac cgcgccaagg tccggcgttc ccgtgtggca gaccgtgatc
     2941 gtggcgtttg cgccggtctg cttttgcaga aggatggcag caagcggctt gccgaccagg
     3001 ttcgaccgcc cgcagatcac gacatggcgt ccatcggggc catgcccgct gcgaacgagc
     3061 agttctcgaa ctcccgccgg cgtacagggc agcaggcgac catctcctcg cagcagcctg
     3121 ccgagattta tcgggtgcag accatcgacg tctttgcgcg ggtcaacggc agccgtgatc
     3181 gctcgctcgt cgatcccgtc gggcaacggc agctggacta tgatgccgtg aaaacgatcg
     3241 tcggcgttga gctggttgac gagattcaat aggtcggcct ggctaatgtc ggggtcaggc
     3301 ctcacggatt ctgacaaaat ttcgagctca gcggcggcac gggttttccc gcggacgtac
     3361 gagactgacg cggcgttgtt acccaccagg acgaccgcga ggccgggtgt gactccggca
     3421 cgtcgcaaca ccgctatgcg cgatgcgagc tccgcccgaa actgcgcggc gacctcgtgt
     3481 ccgttaatca cctcggcggt catgcacctc tccaccgcac ggcgtcggtc aactatagca
     3541 tcagc
//
LOCUS       DATCPE010000010         5335 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U7219
            k141_292444, whole genome shotgun sequence.
ACCESSION   DATCPE010000010 DATCPE010000000
VERSION     DATCPE010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955033
            Sequence Read Archive: SRR1586279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 5335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 16:49:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 640
            CDSs (total)                      :: 631
            Genes (coding)                    :: 627
            CDSs (with protein)               :: 627
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5335
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292444"
                     /isolate="SMAG_U7219"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..864)
                     /locus_tag="VIM45_00300"
     CDS             complement(<1..864)
                     /locus_tag="VIM45_00300"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlkA N-terminal domain-containing protein"
                     /protein_id="HEY8819759.1"
                     /translation="MNTAPTLQSGQVRLHPIQPFNFEYTLTYFTRRAGELVDRAEDGV
                     YRRLFVLGCGPVLAEVRPDADGSLSLNLRPPDGVALSTLDAGAAAAAVASTVTRTLGL
                     ADDLAPFQSTTIADRALAGVVSRYSGLRLVSTPSAFEAFVWAVIGQQISLHVAFRLKA
                     ALVQRFGETADIHSVRYWAFPTPESLAVTDPAELASLGLGRRKAETIVTTAIAVANGS
                     LDLPALALLPLQEAEASLVRMHGVGPWTADYTLLRGLRVADACPVSDMGLRQACGRLY
                     GLGRNATVQEVG"
     gene            927..1385
                     /locus_tag="VIM45_00305"
     CDS             927..1385
                     /locus_tag="VIM45_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018571587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03668 family PPOX class F420-dependent
                     oxidoreductase"
                     /protein_id="HEY8819760.1"
                     /translation="MLSDDQVAYLVGHRVARLATVDKSGQPHAVPVCFAVVDGLIYTP
                     LDRKPKRVAGERLRRVRDLAANPAVCLVVDTYDEDWTRLRWLQVRGTGSLVWINNEQR
                     RAVEALEARYPQYSSMRLEELPVIRITPTDVVEWSWSGRTAYADGGEPLL"
     gene            1396..3972
                     /locus_tag="VIM45_00310"
     CDS             1396..3972
                     /locus_tag="VIM45_00310"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEY8819761.1"
                     /translation="MLELRITDGNETHDEATEKDDVTLGRAPNNDVVLPDKRVSRQHA
                     RLLRRDGGLYIQDLGGANGTQVRDQLLHNETATVTPGEAIVIGPYEVSVETSVPVHGE
                     SVERSLVPPPEVNQQVRKAWESKSTSHVRAIGDADGVQQRYVIPHAPDAARIRLGRAP
                     TNDLVLGSPSVSLEHAVIERSSAGSQWSITSLSSTVPTFVNNVPVRQRGLTEGDVIRI
                     GPYSLVVQNGEIEYVDKAAGILLQALNLSKRVGKKTILHDVSLIIQPREFVAIVGVSG
                     SGKTTLLNASSGISPATSGEVLLNGYQLYDHFASFRSEIGYVPQDDIVHGELPVERAL
                     RYSAQLRMPADTSKSEREKRVDNVIAELGLDQQRKTVINKLSGGQRKRVSIGVELLTR
                     PPLFFLDEPTSGLDPVTDSHMMDLLRKLADQGRTILLVTHATRDISMCDKVTFLTRGG
                     RLAYFGGPRDALQYFGAAEFEEIYDQIENEYSPEEWEERFRQSPDYQSEITTPLVQLQ
                     AMTLPSESRPRPDLSKGNGRQVTGVWRQFGILTRRYVDTMVRDRKHLAILLLQAPIIG
                     LLLWGLFSRSVFEPPINLEVFAWHAATPSVLAIQRDPETNRLINVPPDSDLVAISGRD
                     CSNLTSGNFTVGTECHVAAGNGNSVALKAAQLAFLLAAVAVWLGTLNAIREISKENAI
                     YRRERMVNLRIMPYVASKLAVLWFLVVVQSGMLLGVTAGYVNIPVHIVAATFASLILA
                     GAASVAVALAVSAAVTNSDRAIVLAPLIMVPQILFAGGLTPVRDLGVVKPFSAIVATR
                     WSYEAIGRVFGVVKLAGIAEQFPQTPALTGQPYIPWVVLGAFIIAFSGLALVLQKLKD
                     RH"
     gene            complement(3969..4838)
                     /locus_tag="VIM45_00315"
     CDS             complement(3969..4838)
                     /locus_tag="VIM45_00315"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEY8819762.1"
                     /translation="MPGSSTIHVKLRDPGGHEREVTFDRDVIRIGRARDCDLSLESGF
                     ISRYHARIERAEGGWEVIDEGSKNGVFVNGQRVGTSASQPLHQGDSLRVGDYGLTVDY
                     EEEDDLDRTVIIPPAARRGSAPLEPTAPNEAGMTQPPARPRAAEPAEPPPAPEAVIIV
                     EDPEPVFVPEAAVPEVNEPASAAPASKPPAPLRKAGGGLIVDSAARRVTWDGQPLPSA
                     VSNRELDLLIALTGQSPRSPRELIEAVWGPGGGDDEMLERLVHRTQLKLRPVASDRRL
                     LIQEPDGGYRLGD"
     gene            5015..>5335
                     /locus_tag="VIM45_00320"
     CDS             5015..>5335
                     /locus_tag="VIM45_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018406507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8819763.1"
                     /translation="MVSRERFNYLTGRFAAGGSRMGPKHRENEDCFGIVEPDAPDLRS
                     SHGNLYVVTDGVGGHHKGEVASALAIEVIERAYFAARGDSPIDNLVTAVNEANLRVHE
                     SATLD"
BASE COUNT         1038 a         1684 c         1631 g          982 t
ORIGIN      
        1 gcctacctcc tggaccgtcg cgttgcgccc caacccgtat aaacgaccgc acgcctgccg
       61 caaccccatg tcgctgacgg ggcaggcgtc ggccactctc agacctcgaa gcagagtgta
      121 atcagccgtc cacggtccca ccccatgcat tctgaccagt gaagcctccg cctcctgcag
      181 cggcagcaac gcgagcgccg gcaggtccaa acttccgttc gcgaccgcga ttgcagtggt
      241 cacgatcgtc tcagccttgc ggcgccccag tcccagcgac gccaactcgg ccggatccgt
      301 caccgccagc gattcgggcg tggggaacgc ccagtaacga acgctgtgga tgtcggcggt
      361 ttcgccgaag cgctgcacga gcgccgcttt gagccgaaac gccacgtgca ggctgatctg
      421 ctgcccgatg accgcccaaa caaacgcttc aaacgctgaa ggggtgctga ccagccgcag
      481 gccgctgtaa cgcgaaacga cgcctgccag cgcccggtca gcgattgtcg tcgattgaaa
      541 tggggcaagg tcgtccgcga gccccagcgt acgcgtgacc gtcgaggcaa ccgcagccgc
      601 tgccgcgccc gcatcaagcg tagacagtgc aacgccgtca ggaggtctga ggttaagcga
      661 cagcgaaccg tcggcatcag gccgcacttc tgcaaggacc ggtccgcagc caaggacgaa
      721 gaggcgccgg tatacaccgt cctcggctcg atcgacaagc tcccctgcgc ggcgcgtaaa
      781 gtaggtgagc gtgtactcga agttgaacgg ctgaatggga tgaagacgaa cttgaccgga
      841 ttgcagagtc ggggccgtgt tcatgctgcc cactgtagcc caggagaagc ggaaacgcga
      901 ccgagacggg ctccgaggaa tgcgcaatgt taagcgacga tcaggtcgcc tacctcgtcg
      961 gccaccgagt tgcacgcctc gcgaccgtag acaagtccgg acaaccgcac gccgttccgg
     1021 tctgcttcgc ggtcgtcgat gggctgatct acacgccgct ggaccgaaaa cccaagcgtg
     1081 tagcaggtga gcggctgcgg cgggtacgcg acctggcagc taacccggcc gtctgtcttg
     1141 tggtggatac ctatgacgag gattggacgc ggctacgatg gctgcaggtg cgcggcacag
     1201 gctcgctcgt atggataaac aatgaacaac ggcgggctgt cgaagcgtta gaggcccgtt
     1261 atccgcagta ttcgtcgatg aggttagagg agttgccggt gatccgcata actcccaccg
     1321 acgtagtcga gtggtcgtgg agcggccgaa cggcgtatgc ggacggcggc gagcctctcc
     1381 tatgatcgag ccgtaatgct tgaactacgc atcaccgatg gtaatgagac tcatgacgaa
     1441 gcgaccgaga aagacgacgt caccctaggc agagcgccga acaacgacgt agtcctgccc
     1501 gacaagcgcg tttctcgtca acatgcacgc ttgctgcggc gagatggcgg cttgtatatc
     1561 caggatctcg gcggcgccaa cgggacgcag gtccgcgacc agctattgca caacgaaacg
     1621 gccactgtaa cgcccggcga agcgatcgtg atcggtccgt atgaggtcag cgtcgaaact
     1681 tccgttcctg tgcacggcga atcggtggag cggtcactgg tgccgccgcc ggaggtcaat
     1741 cagcaggtac ggaaagcctg ggaaagcaag tcaacttccc atgtccgggc cattggagac
     1801 gctgatggcg tgcagcagcg atacgttatt ccgcatgccc cggatgctgc gcgtattcgc
     1861 ctgggacgcg cgccgactaa cgatctcgtg ctgggctcac caagcgtatc gctcgaacac
     1921 gccgtcattg agcgatccag cgccgggagc cagtggtcaa tcaccagcct ctcgtctacc
     1981 gttccgacct tcgtcaacaa cgtcccggtt cgacaacgcg ggctcactga aggggacgtc
     2041 attcggatcg gcccgtacag cctcgtcgtt cagaacggag agattgagta cgtcgataag
     2101 gccgcgggca tcctgctcca ggcacttaac ctgagtaaac gcgtcggtaa gaagacgatc
     2161 cttcacgacg tctcgctgat tattcagcca cgagagtttg tcgccattgt tggcgtttcc
     2221 ggctccggca aaacaacgct gctgaacgcg tcaagcggca tcagtccggc aacatccggc
     2281 gaggtgctgc tcaacggcta tcagctctac gaccactttg cctcgttccg ctcagagatc
     2341 ggatacgttc cccaggacga catcgttcac ggcgaacttc cggtcgaaag ggcactgcgg
     2401 tattcggcgc aattgcgaat gcccgccgac acgagtaagt ccgagcggga gaaacgtgtc
     2461 gacaacgtca ttgccgagct tggtctggat cagcagcgta agacggtcat taataaactg
     2521 agcggcggtc agcgcaagcg cgtgagcatc ggcgtcgagc tgttaacacg cccacccctc
     2581 ttcttcctcg acgagccgac ttctggtctc gatccggtca ctgactcgca catgatggac
     2641 ctgctgcgca aactggccga ccagggtcgc accatactcc tggtcaccca tgcgacacgc
     2701 gatatctcaa tgtgcgacaa agtcacattc ctgactcgcg gcgggcgcct tgcttacttc
     2761 ggcgggccgc gcgatgcact gcagtacttc ggtgccgccg agtttgaaga gatctacgac
     2821 cagatcgaaa acgaatactc gccggaggag tgggaagagc gtttccggca gtcgccggac
     2881 taccagtcgg agattacaac cccgcttgtc cagttacagg cgatgaccct cccgtcagaa
     2941 tcgcgtccgc gccctgacct atcaaaaggc aacgggcgtc aggtcacggg agtttggcgc
     3001 cagttcggca tcctcaccag gcgctacgtt gacacgatgg tcagggaccg caagcacctc
     3061 gcgatcctgc tcctgcaggc gccaattatc ggtttgctgc tctggggcct tttcagtcgg
     3121 agcgttttcg agccgccgat taatctcgag gtcttcgcgt ggcacgcggc aaccccttcg
     3181 gtgttggcca tacaacgcga ccccgagact aatcggctta tcaacgtgcc tcccgactca
     3241 gacctcgtag ctatttccgg acgcgattgt tcgaacctaa cctctggcaa ctttacggtc
     3301 ggcacggaat gccacgtagc cgccggaaac ggcaacagcg tcgcgctcaa ggcggcacag
     3361 ctcgcgttcc tactcgctgc cgtggccgtc tggctcggca cgctgaacgc gatccgcgaa
     3421 atctccaagg agaacgcgat ctaccgacgc gagcgcatgg tcaatttgcg catcatgccg
     3481 tatgtcgcct cgaagctcgc ggtgctctgg ttcctggtcg ttgtccagag cggaatgttg
     3541 ctcggagtga ccgccggcta cgtcaacatc ccggtccaca tcgtcgccgc taccttcgcc
     3601 agtctgattc tcgccggcgc tgcatcggtt gcggtcgctc tcgccgtttc ggccgcggtc
     3661 acaaactcgg atcgcgcgat cgttttagcg ccgctgatca tggtgccaca gatcctgttt
     3721 gccggcggct tgacgcccgt ccgcgacctg ggagttgtca agccgttttc ggcaattgtc
     3781 gcgacgcgct ggtcttacga ggcgatcggc cgggtattcg gagttgtgaa gctggccgga
     3841 atcgccgagc agttcccgca aacgccggcg ctaactggcc agccgtacat accgtgggtg
     3901 gtgctcggcg ccttcatcat cgcgttctca ggacttgccc tagtgctcca gaagctgaaa
     3961 gaccggcatt aatcgccaag ccggtagccg ccgtcgggct cctgtatgag gagccggcga
     4021 tcgcttgcga ccggccgcag cttgagttgc gtgcggtgca ccagtcgttc gagcatctcg
     4081 tcgtcgcctc cgccgggccc ccagaccgct tcgatcaact cccgcggcga acgcggcgat
     4141 tgacccgtta gcgcgatcag taggtcaagc tcgcgattgc tcaccgcgct tggcaggggc
     4201 tgcccgtccc aggtaaccct gcgcgcggca gaatcgacga tcaagccacc gccggctttg
     4261 cggagcggtg cgggtggctt cgaagctggt gcggcgcttg ccggctcatt gacttccggg
     4321 acggccgctt cggggacgaa aacgggctcg ggatcttcga cgattatgac tgcctcgggc
     4381 gctggaggcg gttcggccgg ctcagcagcc cggggcctgg ccggcggctg ggtcataccg
     4441 gcctcgttcg gagccgtagg ttccagtggc gctgatcccc gacgcgccgc cggtggaata
     4501 atgacggtgc ggtcgaggtc gtcctcttct tcatagtcca cggtcaggcc gtagtcgccg
     4561 acgcgcaatg aatcaccctg atgcagcggc tggctcgcgg aggtaccgac gcgctggccg
     4621 ttgacgaaga caccgttctt actcccttcg tcgatcactt cccagccgcc ttcagcacgc
     4681 tcgatccggg catggtatcg cgagataaag ccgctttcga gcgacagatc gcagtcgcgg
     4741 gcgcgcccta ttcggatcac gtcccggtca aatgtgacct cgcgttcatg gccaccggga
     4801 tcgcgcagtt ttacatggat cgtggatgac ccgggcatgt ctcacctccg gcccagcgag
     4861 atagcgaaag cgtagatcag aacgcgcgat gcagatagac cgtacctgct attgaagagc
     4921 ccgtgatgcg gcgcagagcg caaacacttg ccaagaacgg cagcgcctcg tagcgtgagc
     4981 atgcaagcta tccctcggag agattactgc gccgatggtc tcgcgcgagc gcttcaacta
     5041 tttgaccggc cgatttgcag ccggtggcag ccgtatgggg ccgaaacacc gcgaaaacga
     5101 ggattgcttc ggcatcgtcg agccggatgc gcccgacctg cgcagttcac acggcaacct
     5161 gtacgtggtc acggacggcg tcggtggcca tcacaaggga gaggtggcca gcgccctggc
     5221 gatcgaagtc atcgagcgag cgtatttcgc cgcgcgcggc gacagtccga tcgacaacct
     5281 cgtgacagcg gtcaacgaag cgaatctgcg cgtacacgag tcggcaactc tggac
//
LOCUS       DATCPE010000011        16589 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U7219
            k141_292476, whole genome shotgun sequence.
ACCESSION   DATCPE010000011 DATCPE010000000
VERSION     DATCPE010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955033
            Sequence Read Archive: SRR1586279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 16589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 16:49:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 640
            CDSs (total)                      :: 631
            Genes (coding)                    :: 627
            CDSs (with protein)               :: 627
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16589
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292476"
                     /isolate="SMAG_U7219"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..929)
                     /locus_tag="VIM45_00325"
     CDS             complement(<1..929)
                     /locus_tag="VIM45_00325"
                     /inference="COORDINATES: protein motif:HMM:NF020318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1786 family protein"
                     /protein_id="HEY8819764.1"
                     /translation="MRILAVDMGTGTQDILLFDSNVAVENCVKMVMPSATEIAARRIR
                     RATVEKRPVLLTGVTMGGGPCHWALDDHIRAGGTAFATQEAARTFDDDLAVVAGMGVA
                     VVSDDEASKLRGIERIEMRDLDLASIRRALSAFEVPSEFDGLAVGCLDHGAAPPGFSD
                     RLFRFNHLRQVIQERNDLKAFAFLPSELPEYLTRARSMISCIDMEVPTVFLDTGPAAA
                     LGALQDPRVAEHDEQVVVNLGNMHALGFYLSGSRVHALFEHHTGLLETPQIESMTEQL
                     MDGTLTHEEVFAGHGHGVFYADRPAGGRPFLAVT"
     gene            complement(1071..2339)
                     /gene="serS"
                     /locus_tag="VIM45_00330"
     CDS             complement(1071..2339)
                     /gene="serS"
                     /locus_tag="VIM45_00330"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEY8819765.1"
                     /translation="MLNPQIIRDNPNLIRRSIELRHTAQPLDDWLKVDNEYRRLLTEV
                     EGKRAAQRVESKKIGATKDPVERQHLIAAVGGLSDEIKTLEVLERELGERRYQLALEF
                     PNLPAEDVPPGEDDHDNVVVRQSGEPKPFDFVALPHWELGERLGVIDFERGVKLSGSR
                     FYVLRGAGAILERALINWMLSLHRRQGYTELYVPFVVKEQCLVAAGQLPKFRDNLYHD
                     AEDNLWLVPTAEVPVTNFHGGEILDGATLPINYTSYTPCFRRERMSAGRDVRGIKRGH
                     QFDKVEMYKFVSPEQSQRELLSLLSDAEETCRRLGLTYRVSQLCGGDLGFAATTTYDI
                     DVWAPGQGEWLEVSSCSNDGDFQARRANIRFRREPGAKPELVHTLNGSGLGLPRTLIA
                     VMENYQQADGSIDVPEVLIPYMDGMTVIGP"
     gene            2476..3552
                     /locus_tag="VIM45_00335"
     CDS             2476..3552
                     /locus_tag="VIM45_00335"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY8819766.1"
                     /translation="MKFGLLYELEYARPWHTGFEREIFHQALDQIELADRVGFDYVWQ
                     VEHHFLTEYSHSSAPEVFLGAVSQRTKRIRIGHGIVLLPMAYNHPVRVAERIATLDIL
                     SNGRVEFGTGRSGTPAELGGFSIDPSLSKEMWDEAVRTIPRMWRDEPFEHHGKFFDMP
                     LRNVVPKPVQKPHPPMWVAAGSPDTFVQAGERGLGVLCFILGRPENLVTRVQSYRNAV
                     RHAEPVGEFINEQVAGFTVTLCLDDEAEAREIGGAAALWYTAMLGSIVGEWRGQCIPG
                     YEYYSHMSQQLSDQAEMDLAGLLDNGTYCIGDAKACIRTIEKYEAAGVDQLICFMQVG
                     RVPHEKIMRSIELFGEKVIPAFRV"
     gene            3577..3966
                     /locus_tag="VIM45_00340"
     CDS             3577..3966
                     /locus_tag="VIM45_00340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8819767.1"
                     /translation="MPTVEETADTFAKDLTSQNIAGLMMVFTPEGMMKAMTMQVQMQA
                     RAVQAISEGRVPAPATGYSLETKEREGDDQVVHITLESADGTAEIMTRWREVEGAWKV
                     TDLALIGARNPDGTPVDLGGGLSPSPA"
     gene            3984..4379
                     /locus_tag="VIM45_00345"
     CDS             3984..4379
                     /locus_tag="VIM45_00345"
                     /inference="COORDINATES: protein motif:HMM:NF016854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HEY8819768.1"
                     /translation="MQWQKTPPELAEQFYTALPADPMVARRKMFGYPSAFVNGNMFAG
                     VHQSTLFLRLAAPNYRELLSIAGSGPFEPMVGRPIKGYASVPESILGSPELLGIWVEK
                     AFADTAALPPKEPKARKPPKAAGRSAKGS"
     gene            complement(4436..4927)
                     /locus_tag="VIM45_00350"
     CDS             complement(4436..4927)
                     /locus_tag="VIM45_00350"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEY8819769.1"
                     /translation="MTGERHFTATGFVVWRGRVLLHWHRKEWLWLPMGGHVEENEDPV
                     QCVLREVEEEAGFKVELFGYRPLRGWSTVREIPAPVTIQVEDIKGSGPLHQHIDMIYF
                     CRPSSPPPAGLPDSTMRWVDVAELEADLSVAPDAQTAPTRIPEDVRVLALDALARSEC
                     GRE"
     gene            complement(4924..6426)
                     /gene="lysS"
                     /locus_tag="VIM45_00355"
     CDS             complement(4924..6426)
                     /gene="lysS"
                     /locus_tag="VIM45_00355"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEY8819770.1"
                     /translation="MSQRNEDLFRQRLAKAEQLRAMGIDPYPARARCTHRAAEALALL
                     DRIESGEAEASFEPAVCGRIRLARSMGKKVSFIDIHDGSGRLQVYCKQEILGGQQFAI
                     IRELDLGDFIQVSGRMFRTRTGEPTLEARSLVVLAKALQPPPEKWRGVTDVELKYRQR
                     YLDLMSSDETRERFRSRSRIVSTIRRYMDARGFIEVETPVLQVEAGGAAARPFKTHFN
                     ALDEERFLRISLELHLKRLLVGGFDKIYEIGRIFRNEGVDTWHNPEFTMMESYEAYAD
                     YHDVARMVEELIATVSREVLGTLQVPHGEILLDLTPPWARMTMRNALIDLAGIDFEGY
                     RDEGALRGWMAGHGIHAKPDLGWGKLLDEVFSELVQPRLQQPVFIIDYPIELSPLAKR
                     KPGDPNLVERFEAFVGGYEIANAYSELNDPVDQRERFERQVTQRARGDDETEMVDEDF
                     LQALEHGMPPAGGLGIGIDRLCMALLNEPTIREVLLFPQLRTKDRSDDEL"
     gene            complement(6544..7017)
                     /gene="greA"
                     /locus_tag="VIM45_00360"
     CDS             complement(6544..7017)
                     /gene="greA"
                     /locus_tag="VIM45_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEY8819771.1"
                     /translation="MTVKAMPMTRDGRARFSAELDHLRNVRRPEVAQRIHEARDEARQ
                     QNNADYEDAKNEQLLIGGRIRELEELLSCAILIEEHQGANDGCVRVGNTVTVVQKTGS
                     EAEYTIVGSAEANPLKGKISNESPCGRALLGRKIGDEVQVMAPSGAITITVKRIE"
     gene            7294..9045
                     /locus_tag="VIM45_00365"
     CDS             7294..9045
                     /locus_tag="VIM45_00365"
                     /inference="COORDINATES: protein motif:HMM:NF012907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HEY8819772.1"
                     /translation="MSRRSPGPLMSRTGSQSRSGIGSKGNRRPLLFVGGGAVLALLII
                     VFAFSRCGGGSSGQAAECAGKAPTPPPGYSYASKCYSPLKEFAGKRGSLSIPLTDKSS
                     NRGLTLFSHDSGAWKPIGPVQVSADGTSAVVASAIDIPKTFAVLRRTGGDFQIFGELP
                     PNGTPSADASRLLTEAIPVAYAPTADGGISGGPLTRPVGAGYGLMPAVAAPPGGTEAQ
                     AIDTILSDTNRLNAHVERIAAEVDRNQYDGIVIDYGSVNPSLKTNFTTFVQALATRLH
                     ASKKRLVLRLPLPRREGTNWNTLAYDWPAIGKSADNVILAAEHDQSIYRQRVPEAVKY
                     LVGQVGDSRKLVLEITPLSEEKSDQGQIRLLSTLEALSIAGQITVRDGNTVGSGTDVV
                     VSADNVNRENGSGPQWTPQGVVSFQYRSGDQQRTVWIENLFSVGYKLELIQFYKLGGA
                     SVDNATADPAVTNIWPAIDQYQSTSSPLLQQPNPQSLRPQWLADGRPIPDAGNRAQIT
                     WRAPDQPGKYTLTVVVSDGTMRVADSTTVDARAAVPGATPGPTPRAGASVPSVRPTQT
                     STRPAAAPSRAPSSPPR"
     gene            9067..9633
                     /gene="rsmD"
                     /locus_tag="VIM45_00370"
     CDS             9067..9633
                     /gene="rsmD"
                     /locus_tag="VIM45_00370"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEY8819773.1"
                     /translation="MRVIAGEARGRILHGPKSAGTRPTSDLVRGAVFSMLESRGASFT
                     RVLDLFAGTGALGIEALSRGADWADFIESDRAACGVIAANLVSTGFQQRAAVVCATLP
                     VALMRVRGSYGLIFVDPPYGLDNFSAVWVRLRESGTIDPETTIAYEHSRRTIPPERCG
                     PLPRIVTRAHGTTAVSLYYAGEPETRDP"
     gene            9644..10120
                     /gene="coaD"
                     /locus_tag="VIM45_00375"
     CDS             9644..10120
                     /gene="coaD"
                     /locus_tag="VIM45_00375"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014185910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEY8819774.1"
                     /translation="MTIAIYPGRFDPVTNGHLDIVRRAVKLFGVVIVGVAASRSAVFT
                     IDERVKLFRQAVADQPNVSVKPIPGLTVDFAIEHGATVLVRGIRAVTDFEAEIDMALM
                     NKKMVPEIESVYLMTSLEYLYVRASLIREITGLGRDPSEFVPAHVVRALKEKFAIT"
     gene            10089..10676
                     /locus_tag="VIM45_00380"
     CDS             10089..10676
                     /locus_tag="VIM45_00380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8819775.1"
                     /translation="MPSKKNSPSRKDDRTAREVGAVDILHLLDQLEEEVASARKLPMG
                     GGVLVDRKRMLELVDQLRVAIPANIRQARDVMQSREKTLTDADNEARRIFAAAHQEAQ
                     RLVADDAITREARSLAERTQREAQVQAHSTLREAQQQAEQELAQAKQTARQQVEDADQ
                     YTLAVLTRMDKQISAFLGNVRQSIEALQGDGGNEQ"
     gene            complement(10749..13217)
                     /gene="leuS"
                     /locus_tag="VIM45_00385"
     CDS             complement(10749..13217)
                     /gene="leuS"
                     /locus_tag="VIM45_00385"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEY8819776.1"
                     /translation="MAKQARDIGRYKPGDIEPKWQQRWERDGLYRTVDDDPRPKYYFL
                     TMFPYPSGDLHLGHWYAEAPADAGARYLRMRGYNVLFPMGFDAFGLPAENAAIKNKIH
                     PLTWTLANIGRMRDQFRTMGAMFDWSREVDTCLPEYYRWNQWFFLQFLKQGLAYRQMA
                     AVDWCPNCNTTLAREQVIGEKRTCERCDTPVIKRDLEQWFLRITKFADRLLNFDGLEW
                     PEHVMTMQRNWIGRSEGADLEFTALLADGTQRPLRVFTTRPDTVFGATFMVLAPEHPL
                     VAEITTPDRRAEVEAYVEGATRETEIERSAVDKEKTGVFTGGFCINRFNGEQIPVYIA
                     DYVLMGYGTGAIMAVPAHDQRDFEFATKYAIEIRPVFSVLEGNGSRYTRAITHGEATL
                     NSGSFDGLAEDVAKRAITEYAEREGIGSAAVTYRLRDWLISRQRYWGTPIPIIYCPTD
                     GAVPVPEADLPVLLPDDAEFLPTGESPLKFHHKFRYVSCPKCGGPAERETDTMDCFVD
                     SSWYWYRYLSPHDDSAPFDQAAARDWLPVAQYTGGIEHAILHLMYSRFWTKAMKDLGL
                     TENEEPFTRLFNQGMILGADGQRKMSKSRGNVLNPDNQVARYGADAVRCYLMFIGPWS
                     EGGPYNMQGIDGVSRWLNRVWRLVHEPAPSGSVDAGVVRDLQRLTHKTIKRVGDDIDV
                     MRFNTMIAALMEYTNGLTKARESGIEGATWSKAIDTLLLLIAPSAPHFAEELWEQRGR
                     PYSIHQQSWPAFDAGLAADDVVVVAVQVNGKLRDRLTVPADAAEATVREMALASDRVQ
                     AQLDGHAIARIVYVPGRLLNVVVE"
     gene            complement(13546..13618)
                     /locus_tag="VIM45_00390"
     tRNA            complement(13546..13618)
                     /locus_tag="VIM45_00390"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13583..13585),aa:Ala,seq:ggc)
     gene            13786..14988
                     /locus_tag="VIM45_00395"
     CDS             13786..14988
                     /locus_tag="VIM45_00395"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEY8819777.1"
                     /translation="MASLALEGIKVLDLSRLAPGPYCTMLLGDLGADVLLIEEAGGGS
                     SRRAGSMPGRAGGSEAAERAAAYNALGRNKRSIGLNLKNEQARQVFYDLCRDADVVLE
                     GFRPGVVKRLGVDYETISAINPRIVYCSLSGYGQTGPYSQVVGHDINYISIAGALGAI
                     GWPNTPPAIPLNLLADFAGGGLQAAFAIVVALLARNRIGHGQYVDIAMSDGVISLLAS
                     AASGFFGSGLVPRRGQHTLNGAVPYFNVYETADGKWFSIGAIEPWFYANLCKVLGCEQ
                     YTDYQHDQSKYAEIKAYFSEQFKTRTRDEWFRVLTEVDICAAPVYSLDEVFEDPHNRF
                     RQMQVEVEHPKFGKVKQVGIGTKFSETPGKVRSTAPMNGQHTDEVLRGLGMDEDRITR
                     LRAEGGIA"
     gene            15297..15857
                     /locus_tag="VIM45_00400"
     CDS             15297..15857
                     /locus_tag="VIM45_00400"
                     /inference="COORDINATES: protein motif:HMM:NF015298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HEY8819778.1"
                     /translation="MQKHLAVALVAALTMSGLTAGRAFADHEIVEQPGDTMQSNSEHG
                     DISSLSAASGPATGFTLPILIGPSSPDSPPGIGFSSPELPDDAVIPGRPIQYGLATWY
                     GPGFDGHLTYCGDVYDEAAYTAASNTLPCGAVVIVTNQDNGASVRVRITDRGGFGSAV
                     ILDLSRAAFGTIASNGSGVIAVMVSQ"
     gene            complement(15965..>16589)
                     /locus_tag="VIM45_00405"
     CDS             complement(15965..>16589)
                     /locus_tag="VIM45_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8819779.1"
                     /translation="MDPQVIVDRLVRLVRLDTTVFEEARDDLAGTIPALVIAVISFFL
                     CGLGGWIWWLTQGYGDKAKVFWQSALLGSLIAFGFWVVWVLVAYGVLVYVFRYTANVE
                     RMIRACSLATIPVALSLIMFIPGVNLGIGLASFALMFLLMDIGIQVSVEAQPGHVIAA
                     TFLGFAVFCVLLSLFVQSGDAFAPGIFLFRAPASILSDVTKALSGFR"
BASE COUNT         3267 a         5181 c         4922 g         3219 t
ORIGIN      
        1 gtaaccgcaa gaaacggcct cccgcccgcg ggacggtcgg cgtagaagac accgtgcccg
       61 tggccggcga agacctcttc gtgcgtaagc gtgccgtcca tcaactgttc ggtcatcgat
      121 tcgatctgcg gcgtctccag cagcccggtg tggtgctcga acagcgcgtg aacgcggctg
      181 ccactgaggt agaagccaag cgcgtgcata ttgccaaggt tgacgactac ctgctcgtcg
      241 tgctcggcaa cccgcgggtc ctgcagagcg ccgagtgcag ccgccggtcc cgtatcgagg
      301 aagacagtag ggacttccat gtcgatacag ctaatcattg aacgagcgcg cgtcaggtac
      361 tccggcaact cggatggcag gaacgcgaag gctttcagat cgttacgttc ctggatcacc
      421 tgtcgcagat ggttgaaccg aaagagccgg tcggaaaagc cgggcggcgc tgcgccgtgg
      481 tctaggcagc cgacagcgag accatcgaat tcagacggca cctcgaaggc agaaagcgcg
      541 cggcggatcg atgcgaggtc aaggtcgcgc atctcgatcc gctcgatgcc gcgcagcttc
      601 gacgcctcgt cgtccgacac taccgctacg cccataccgg caacgaccgc gaggtcatcg
      661 tcaaacgtgc gggcggcttc ctgggtcgcg aacgccgttc caccggcccg aatatggtcg
      721 tcaagcgccc agtggcaggg ccctccgccc atggtcacgc ctgtcaacaa taccggacgt
      781 ttctcgaccg tggcgcggcg gatgcgccga gccgcgatct ccgttgcaga cggcatgacc
      841 atcttgacgc agttttcgac ggccacgttg gagtcgaaca ggaggatatc ctgcgtcccc
      901 gtgcccatgt ctacggctaa gatgcgcatc gcgcggctcc gaatgtcggc gagagcgcga
      961 ccggcgcccc tgctacactt ggattcatca tgccacgtac accggcctcg ttgggccaat
     1021 gaaacagaca tctgaggtag ttacgttgcg ttctgttggc tctccggtgc ttatggcccg
     1081 atgacggtca tgccgtccat gtacggaatc aatacctcgg gaacgtcgat actgccgtca
     1141 gcttgctggt aattttccat aacggcgatg agggtgcgcg gcagccccaa gcccgacccg
     1201 ttgagcgtgt ggacgagctc cggcttcgcg ccgggctcgc gccgaaagcg gatgttcgcg
     1261 cgccgcgctt gaaaatcgcc gtcatttgag caggaactga cttcgagcca ttcaccctgg
     1321 ccgggtgccc agacgtcaat gtcgtaggtc gtcgtcgccg caaatccgag gtcgcctccg
     1381 cagagttgcg aaacgcggta ggtcaggccc aggcggcggc acgtctcttc ggcgtctgag
     1441 agcagcgata gaagctcgcg ctgcgactgc tctggactga cgaacttgta catctccacc
     1501 ttgtcgaact gatgaccccg tttgatgccg cgcacgtctc tgcctgcgct catccgctcc
     1561 cggcgaaagc aaggggtata actggtgtag ttgatgggca gcgtggcgcc atccaggatc
     1621 tctccgccgt gaaaattcgt caccggcact tcggcggtag gaacgagcca aaggttatct
     1681 tcggcgtcgt ggtaaaggtt gtcacggaac ttcggcaact gtcctgccgc gaccaggcac
     1741 tgctctttca cgacgaacgg tacgtacagt tcggtatagc cctggcgccg gtgcagagag
     1801 agcatccagt taatcagcgc ccgttccaga atggccccgg ctcccctgag cacgtagaag
     1861 cgcgaaccgg atagcttcac gccgcgctcg aagtcgatga cccccaatcg ctcgccgagc
     1921 tcccaatggg gtagcgccac aaaatcgaag ggtttcggct cgccggactg acgcaccacg
     1981 acgttgtcat ggtcgtcctc tcccggcggc acatcttcgg cgggaaggtt ggggaactcc
     2041 agagcaagct ggtagcgccg ctcgccaagc tcgcgctcca ggacttccag ggtctttatt
     2101 tcgtcgctca gaccgcccac ggcggcaatg aggtgttggc gctccacggg gtctttggtt
     2161 gcgccgattt tcttgctttc aacacgctgg gcagcccgct tgccctcaac ttcggtcagc
     2221 aggcgacggt actcgttgtc aaccttcaac cagtcgtcca gcggctgagc cgtgtgacgc
     2281 agctcgattg aacgacggat cagattgggg ttatcgcgga tgatctgtgg gttcaacatc
     2341 ggttggagtg tctggtatgc ccggcggcca cgtcaacgca agtgaccatt cggaggggcg
     2401 tgccgcatct cattgacgca gcccgaaccc cgatggacac tggctaatgg gcgcccccgg
     2461 tcgggagggg aaggcgtgaa attcggactg ctgtacgaac tcgaatacgc gcgcccatgg
     2521 cataccggat tcgagcgaga gatatttcac caggccctgg accagattga gctcgccgac
     2581 cgtgtgggct tcgattacgt ttggcaggtc gagcatcatt tcctcaccga gtactcgcac
     2641 tcttccgcac cggaagtctt cctcggcgcc gtctcgcaac gaacgaagcg gatcaggatc
     2701 ggccacggga tcgttctgct gcctatggct tacaaccacc cggttcgcgt cgcggaacgg
     2761 atcgcgacac tcgatatcct gtcgaatggg cgcgtggagt tcggcaccgg ccgctcgggc
     2821 acaccggcgg agcttggcgg attcagtata gatccttcgc tatccaaaga gatgtgggac
     2881 gaagcggttc gcacgattcc caggatgtgg cgcgacgagc cgtttgaaca tcacggaaag
     2941 ttcttcgaca tgccgctgcg gaacgtcgtg ccgaagcctg tgcaaaagcc gcatccgccc
     3001 atgtgggtcg cggcgggcag cccggatacc ttcgtgcagg cgggcgagcg cgggcttggc
     3061 gttctctgct ttatactcgg tcgcccagag aacctggtta cccgcgtgca gtcgtaccgc
     3121 aatgcggttc gccatgccga acccgtcggc gagtttatca acgagcaggt ggcgggcttc
     3181 acggtgacac tctgcctgga cgacgaagcc gaggcgcggg agatcggcgg ggcggcggcg
     3241 ctgtggtaca cggccatgct tggctcgatc gtcggcgaat ggcgcgggca gtgcatcccc
     3301 ggctacgagt actactctca tatgagccaa cagttgagcg accaggcgga gatggacctc
     3361 gctggtctgc tggataacgg cacgtattgt attggcgacg ccaaggcgtg catccgcacg
     3421 atcgagaagt acgaggccgc cggagtggat cagttaatct gcttcatgca ggtcggacgc
     3481 gtgccccacg aaaagatcat gcgctcgatt gagctgttcg gcgaaaaggt catacccgcg
     3541 ttccgggtct agtacccggc gcgccaggag atgcttgtgc caaccgtcga ggaaacggca
     3601 gatacgtttg ccaaagatct cacttcgcaa aatatcgccg gactgatgat ggtcttcacg
     3661 cccgaaggaa tgatgaaggc tatgacgatg caagtccaaa tgcaggcgcg tgcggtgcag
     3721 gccatatccg aaggccgggt gcccgcccct gcaaccgggt attcgctgga gacgaaggaa
     3781 cgagaaggcg atgaccaggt tgttcacata acgctagagt ctgccgatgg aactgccgag
     3841 atcatgaccc ggtggcggga ggtcgagggg gcttggaaag ttacggacct ggcgctgatc
     3901 ggagcccgaa atccagacgg aacacctgtt gaccttggag gcggactctc gccttcacca
     3961 gcctgataaa ggagcagtca tccttgcagt ggcaaaaaac gccgcctgaa ctcgccgagc
     4021 agttctacac cgcacttcca gctgatccca tggttgcgcg gagaaaaatg ttcggctacc
     4081 cgagtgcttt tgtgaacggc aatatgttcg ccggagtcca tcaatcgacc ctattcctcc
     4141 ggctagccgc acccaactac cgagaactcc tgtcaattgc cggctcaggg ccattcgagc
     4201 cgatggtcgg gcgtccgatc aagggctatg cgagcgttcc ggaatcgatt ctcggtagcc
     4261 ctgagcttct cggcatctgg gtggagaaag cgttcgcgga cacggctgcg cttccaccaa
     4321 aggaaccgaa ggcgcgcaaa ccgccaaagg ccgccggacg gtcagcgaag ggctcctaac
     4381 gacctcactc cgctgctcaa ttgtgccgat tcgaggcggc agcaaccacg gctcgttatt
     4441 cccggccgca ttccgatcgc gctaatgcat ccagcgccag tacccttacg tcttcgggga
     4501 tgcgggtggg cgccgtttga gcatcgggag ccaccgacag atctgcctcc aactccgcca
     4561 cgtcgaccca gcgcatggtt gagtcgggca gtccggcagg tggcggagat gacggccggc
     4621 aaaagtaaat catgtcgatg tgctggtgga gcgggccgct acctttgatg tcttcgacct
     4681 gtatcgttac cggcgccggt atctctcgta cggtgctcca tccccgtaat ggccggtatc
     4741 cgaagagctc gaccttgaag ccggcctctt cctccacttc ccgtagaacg cactgcaccg
     4801 gatcttcgtt ctcttcgaca tgaccgccca tcggcagcca caaccactct ttccggtgcc
     4861 agtgcaggag cacccggccc cgccagacca cgaatccggt agcggtaaaa tgacgttcgc
     4921 cggtcacaat tcatcatccg agcggtcctt ggtccgcagc tgcggaaaga gcaacacctc
     4981 gcgaattgtg ggctcgttca acagcgccat gcagaggcgg tcgatgccga tgccgaggcc
     5041 gcccgccggc ggcatgccgt gctcgagcgc ctggaggaag tcttcgtcga ccatctcggt
     5101 ttcgtcgtcg ccccgtgctc gctgggttac ctgccgctca aatcgctcgc gttggtcgac
     5161 gggatcgttc aattcgctat aggcgttggc gatttcgtag ccgccaacga acgcttcgaa
     5221 tctctcgacc aggtttgggt cacccggctt acgtttggcc aggggtgaaa gctcaatcgg
     5281 ataatcgatg atgaacacgg gctgctgcag tcgtggctgc acgagctcac tgaaaacctc
     5341 gtctaataac ttcccccagc caagatcggg cttcgcatgg atcccgtggc cggccatcca
     5401 cccacgcagc gccccttcgt cgcggtaccc ctcgaagtca atacccgcga ggtcgatcag
     5461 ggcgttgcgc atcgtcatgc gcgcccaagg cggtgtcaga tcgagcagga tttcgccgtg
     5521 gggaacctgc aacgtaccca ggacttcccg cgaaaccgtc gctatgagct cctcgaccat
     5581 cctggcaacg tcgtggtaat ccgcatacgc ctcgtagctc tccatcatcg tgaactcggg
     5641 gttgtgccag gtatctaccc cctcgttgcg aaagattcgc ccgatctcgt agatcttgtc
     5701 gaagcctcca accaacaggc gcttcaggtg tagctcgagt gagatgcgca ggaaacgctc
     5761 ttcgtcgagt gcgttgaagt gtgtcttgaa aggccgcgcc gcggctccgc ccgcctcgac
     5821 ctgcaacacc ggcgtctcga cctcgatgaa tcctcgcgcg tccatatacc ggcggatcgt
     5881 agagacgatg cggctgcggc tcctaaagcg ttcgcgcgtt tcgtcactcg acataaggtc
     5941 caggtagcgc tgccgatact tcaattcgac gtcggtgaca ccgcgccact tctccggggg
     6001 cggctgcagc gcctttgcga gcacgaccag gctacgcgcc tcaagggttg gttcacccgt
     6061 tcgagtgcga aacattcgcc ccgaaacctg aatgaagtcg cccaggtcga gctcgcgaat
     6121 aatggcgaat tgctgccctc cgaggatctc ttgtttgcaa tagacctgta gccgtccgct
     6181 gccgtcgtgg atatcgatga agctaacttt cttgcccatg gaacgggcaa gacggatgcg
     6241 gccgcagaca gccggctcaa agctcgcttc ggcttcaccg ctctcgattc ggtcgaggag
     6301 cgccagcgct tccgccgcgc gatgggtgca gcgggcacgg gcagggtatg ggtcgatgcc
     6361 catagcacgc agctgctcgg ccttggccag ccgctgtcgg aacaggtctt cgtttcgctg
     6421 gctcatttcc cgctcttcag gcagataggg cacgcggagt cacgggcagg tcatagaacg
     6481 tgcaacaaaa tagcggggcc gacacgcgcc gccccgctac gggccgtgcc atccataccg
     6541 cgattattcg atacgtttga ccgtgatcgt aatagccccg gatggcgcca tcacctgcac
     6601 ttcatcgcct atttttcggc ccagcagggc tctgccgcat ggcgactcgt tgctgatctt
     6661 gcctttcaac gggtttgcct cggcactccc cacgatggtg tactctgcct cgctacctgt
     6721 tttttgcacc accgtcaccg tgttcccaac acgaacacag ccatcgttcg cgccctggtg
     6781 ctcctcaata agaatggcgc acgacagcag ctcttcgagc tcgcgaatcc gccccccgat
     6841 gagcaactgc tcgtttttgg cgtcttcgta atctgcattg ttctgctggc gcgcttcatc
     6901 gcgcgcctcg tggatacgct gcgctacctc cggcctgcgg acgttacgca gatggtcgag
     6961 ttccgccgaa aaccgggcgc ggccgtctcg cgtcatcggc atggccttga ctgtcatggt
     7021 caactcctac tggcccattg ccagccactt agcaaatgga ctgaggcacg gttttcgctc
     7081 cccagtccag cctgcccgat ggcggcgcaa tcatacgatg ggccccaaac ggtgtcaacg
     7141 aacgtcgctt gccgtgatgt gtcgatacta atccgtgatt cacaagggtg cggatgttag
     7201 aatcgtgtaa cctcctcgct tctgcatctg cgaagcaggc acatgagtgc cggcaggctg
     7261 caacgatgac gaatcctcta ttggagcggt caattgagcc gtcgatcacc gggaccgctg
     7321 atgtccagga ctggcagcca gtcacggtcc gggatcggct ccaaaggaaa tcgccggccg
     7381 ttattgttcg tcggcggtgg tgctgttctc gctctgctga tcatcgtgtt tgctttttct
     7441 cgctgcggcg gcgggagctc cggccaggcc gcggagtgcg cgggcaaggc tcctacccct
     7501 ccaccggggt attcgtacgc cagtaagtgt tattcgcctc tgaaagagtt tgccggtaaa
     7561 cgcgggtcgc tcagcatccc gctgacagat aagtcgagca accgcggcct gacgctattc
     7621 agccatgaca gtggcgcttg gaagccgatc ggtccggtgc aggtttcggc cgacgggacc
     7681 tcggcggtcg tcgcctcggc gatcgacatc ccgaaaacgt tcgccgtcct ccgtcgaacg
     7741 ggaggcgatt ttcagatctt cggcgaatta ccgccaaatg gcacaccatc cgcggatgca
     7801 tcgcggttac tcaccgaagc gatcccagtg gcgtatgcac ctacagccga tggcggcatt
     7861 agcggcggcc ctctaacgag accggttggc gctgggtatg gcctgatgcc cgccgtcgca
     7921 gcgccgccag gcggcaccga agcccaggcc atcgatacga tcctgagcga cacgaatcga
     7981 ctcaacgcgc acgtcgaacg gattgcagcc gaggtcgacc ggaaccagta cgacggtatc
     8041 gtcatcgatt acggcagcgt caacccgagc ctcaaaacga acttcacgac ttttgttcag
     8101 gcgctcgcca ctcggctgca cgcatcaaag aagcggttgg tgctgcgatt gcctctgccg
     8161 cgccgcgaag gcacgaactg gaacacgctc gcgtacgatt ggcccgctat cggtaaatcc
     8221 gcggacaacg tcatcctggc ggcggaacac gaccaatcaa tttatcggca gcgtgtgccc
     8281 gaagctgtta aatacctcgt cggccaggtt ggtgactccc gcaagctcgt tctggaaatc
     8341 acccctcttt ccgaagagaa gtccgaccag gggcagatcc ggctgttgag cacgctcgaa
     8401 gccctcagta tcgccggaca gattacggtc agggatggca atacggttgg ttctgggacc
     8461 gacgtcgtgg tgtcggccga caacgtgaac cgcgaaaatg gcagcggacc gcagtggacg
     8521 cctcagggcg tcgtgtcgtt ccagtaccgc agcggcgacc aacaacgcac cgtttggatt
     8581 gagaacctgt tcagcgtggg ttataagctg gagttgatcc agttctacaa gctcggtggg
     8641 gcctcggtag acaacgccac tgcggacccc gcggtcacca acatctggcc tgcaatcgat
     8701 cagtatcagt ccacgagctc ccccctcctc caacagccga atcctcagtc gctgcgtcct
     8761 caatggctcg cagatggcag gccgattccg gatgccggca acagggccca gattacctgg
     8821 cgcgcccccg accagcccgg caaatacact ctgacggtgg tcgtcagcga cggaacgatg
     8881 cgcgttgccg actccacgac cgtggatgcg cgtgctgccg ttcccggtgc gacgcctgga
     8941 ccgactccac gcgccggtgc cagtgttcca tccgtacgac caacgcaaac atcaactcgt
     9001 cctgcggcgg ccccaagccg agcgccgtct tcccctccac gctgacgctg agtcgagcag
     9061 gccggtatgc gcgtcatcgc aggcgaagcc cggggccgga tattacatgg gccgaaatcg
     9121 gcgggcacgc gacctacttc ggacctcgtg cgcggcgctg tcttttcgat gctcgaatcg
     9181 cgcggagcat cgtttacgcg tgtgctggat ctgtttgcag gtacgggcgc tcttggcata
     9241 gaggcgctga gccgcggcgc cgattgggcc gatttcattg aaagcgaccg cgccgcctgc
     9301 ggagtgatcg cagctaatct cgtcagcacc ggtttccaac agcgcgcggc cgtcgtctgt
     9361 gcgaccctgc ctgtcgcact gatgcgcgtg cggggttcgt acggtctaat ctttgtcgac
     9421 ccgccgtacg gtttggacaa tttctccgcg gtctgggttc ggctgcggga gagcgggacc
     9481 atcgaccccg aaaccactat cgcctacgag cacagccgcc gtacgattcc tccggagcgt
     9541 tgcggtccgc tgccgcgcat tgtcacacgg gcccacggga ccacggccgt gagcctctac
     9601 tacgccggcg aaccggaaac ccgcgacccc taaggagcgt cttttgacga tcgctatcta
     9661 tcctggcagg ttcgatccgg tgacgaacgg ccatctcgac atcgtccgga gggctgtgaa
     9721 gctctttggc gtggtcattg tcggtgtcgc cgcgagcagg tcggcggtct tcactatcga
     9781 tgagcgagtt aaactcttcc gtcaggctgt ggcagatcag ccaaacgtga gcgtaaagcc
     9841 cattccggga ctcaccgtcg attttgctat cgagcatggc gcaactgtgc tggttcgcgg
     9901 catccgcgcg gttacggatt tcgaggcaga gatcgacatg gcgttgatga acaagaagat
     9961 ggttcccgaa atcgaatccg tgtatcttat gaccagtctc gaatatctct atgtccgcgc
    10021 aagtctcatc cgggaaataa cgggcctggg ccgtgacccg tccgagtttg ttccggcaca
    10081 cgttgtccgt gccctcaaag aaaaattcgc catcacgtaa agacgatcgc acagcccggg
    10141 aggtaggcgc cgtcgatatc cttcatctgc tggaccagct cgaagaagag gtcgcctcag
    10201 cgcgcaaatt gcccatgggc ggcggggtgc ttgtcgatcg caagcggatg ttggaactgg
    10261 tcgaccagtt gcgagtggcg attccggcga acattcgcca ggcgcgcgac gtcatgcaga
    10321 gtcgcgaaaa gactctgacc gatgccgaca acgaagcccg ccggatcttc gctgcggccc
    10381 accaagaagc ccaacgcctg gtcgccgatg atgcaatcac gcgcgaggca cggtcacttg
    10441 cggagcgcac gcaacgagag gcgcaggtgc aggcgcacag cacgttgcgg gaagcccagc
    10501 agcaggctga acaagagttg gcccaagcta aacaaacggc ccgccaacag gttgaagacg
    10561 ccgatcagta cacgctggcc gttcttaccc gcatggacaa gcagatttct gctttcctcg
    10621 gcaatgtccg acagagcatt gaggcccttc agggcgatgg gggcaatgaa cagtaggcgg
    10681 gccgtctatc cgcagcactg ttccacgcca ctggccatag ccccaacgac tgccgaccgc
    10741 cgactgcttc actccaccac gacattcaag aggcggccgg gcacgtacac gatccgcgcg
    10801 atggcatgtc cgtccaattg ggcctggaca cggtcgctcg cgagtgccat ctcgcgcacg
    10861 gtagcctcgg cggcgtccgc gggaaccgta agccggtccc gcaactttcc gttcacctga
    10921 acggctacaa ccacgacgtc gtcggctgca agcccggcat caaacgccgg ccagctctgc
    10981 tggtgaatcg aatacgggcg cccgcgttgc tcccacagct cctccgcgaa gtggggtgcg
    11041 gagggggcaa tcaggagcag caaggtgtcg atcgctttag accaggtcgc cccttcaatt
    11101 cccgactcac gcgccttggt caggccgttg gtgtactcca tcaatgccgc aatcatggtg
    11161 ttgaaacgca tgacgtcgat atcgtcgcca acccgcttga ttgttttgtg cgtcagtcgt
    11221 tgcaggtcgc gtaccactcc ggcatcaacg cttccagacg gggcaggctc gtgcaccagc
    11281 ctccagacgc gattcagcca gcgactgacg ccgtcgatcc cttgcatgtt gtacggcccg
    11341 ccttcgctcc acggtccgat aaacatcagg tagcagcgca ctgcatcggc cccgtagcga
    11401 gcaacctggt tgtcggggtt gagaacgttc ccgcgcgact tgctcatctt cctctggccg
    11461 tcagcaccca ggatcatccc ctggttgaac agccgtgtaa acggctcttc gttctccgtc
    11521 aatcccaggt ccttcatcgc ctttgtccag aagcgtgagt acatcaggtg caggatcgcg
    11581 tgttcgattc cgcccgtata ctgcgcgacc ggcagccagt ctctggccgc agcctggtca
    11641 aacggcgcgg aatcgtcgtg cgggctcaag tagcgatacc agtaccatga cgagtcgacg
    11701 aaacagtcca tggtgtcggt ttcgcgctcc gccggtcccc cgcacttcgg gcagctcacg
    11761 tagcgaaact tgtggtggaa cttcaaaggc gactcgccgg tgggcaaaaa ttccgcgtca
    11821 tctggcagca gtaccggcag gtcggcctcg ggcaccggca cggcgccgtc tgtggggcag
    11881 taaatgatcg ggatcggtgt gccccagtac cgctggcggc tgatcagcca gtcgcgtagc
    11941 cggtaggtta cagcagcact accgatcccc tcacgttcgg catactccgt gatcgcacgc
    12001 ttcgcgacgt cttcggccag accatcaaac gaccccgagt tgagggtggc ctcgccatgg
    12061 gtgatcgcac gggtgtaacg agagccgttg ccttccagca ccgaaaagac cggccgaatc
    12121 tcaattgcat acttcgttgc gaactcaaag tcgcgctggt catgtgcggg aaccgccatt
    12181 atcgccccgg tgccgtatcc catcagcacg tagtcggcga tatacacggg gatctgctcg
    12241 ccgttgaagc ggtttatgca gaatccgccc gtgaacacac cggtcttctc tttgtcaacg
    12301 gcgctgcgct cgatttcggt ctctcgagtg gcgccttcca cgtacgcctc tacttcagcc
    12361 cgccgatcgg gtgtcgtgat ctccgcgacc agcggatgct cgggggcgag gaccatgaag
    12421 gtcgcgccga aaaccgtgtc gggccgggtc gtaaagacgc gcagcggcct ctgcgttccg
    12481 tccgccaaca acgcagtgaa ctccaaatcg gccccttcac tacgaccgat ccagttccgt
    12541 tgcattgtca taacgtgttc cggccattcg agcccgtcga aattcaacaa acgatcggcg
    12601 aacttggtaa tccgcagaaa ccactgctcc agatcccgct taataaccgg cgtatcgcag
    12661 cgctcgcagg tacgtttttc ccctatcacc tgctcgcggg cgagggtggt attgcagttc
    12721 ggacaccagt ccactgccgc catctggcgg taggcaagac cttgcttcaa aaactgaagg
    12781 aagaaccatt gattccagcg gtagtattct ggcaggcaag tatccacttc gcgcgaccaa
    12841 tcgaacatgg cgcccatggt gcggaactgg tctcgcatgc gaccgatgtt ggcgagcgtc
    12901 caggtgagcg gatggatctt gttcttgatc gccgcgttct ccgcgggcag cccgaacgcg
    12961 tcgaagccca tcggaaacag cacgttgtag ccgcgcatgc gtaggtagcg cgcgcccgcg
    13021 tccgccggcg cctccgcgta ccagtgaccg agatgcaggt cgccggaagg gtacgggaac
    13081 atggtcagga agtagtactt agggcgcggg tcgtcgtcga ccgtccggta tagaccgtcc
    13141 cgctcccagc gctgctgcca tttaggttcg atgtcgcccg gtttgtaacg gcctatatct
    13201 cgtgcttgct ttgccatcgc cctcacccaa atctgccgaa caaaaaaccc tccgtccact
    13261 cagggacgga gggcataagc gccttactcc gcggtaccac cctggttggt ccctgcgctc
    13321 cgaattcgaa ccggagacgt ctcgctgaaa cccgcctttg ggggcgcgat aacgggcgct
    13381 acccgtcggc cgctaccctg cctaccggca gattcacgac cgccactccc aggcgagttc
    13441 ggccccggtg ccgctttgca ccacccaacg gctctctgct gaagggacct actactcctg
    13501 atcagcgcgt attcttgtct attcctctgg tcctgcatgc gccggtggag gtgaggggat
    13561 tcgaacccct tacctcaaca ctgccagtgt tgcgctctcc cgattgagct acacccccga
    13621 tgcccgaaaa tcgtatcaaa acggctgtcc ggcggcaagc tgttggtgag gtggcgcggt
    13681 tccaacgggc taacgcacgg cttgacaggc acttccagcg gcgcggatga tccggactgc
    13741 ccgccggtgg aacctcgtgg ccggcaagct gaaaggagga caacagtggc gtccctggcg
    13801 cttgaaggaa taaaggtact cgacctttct cgccttgctc ccggtcccta ttgcacaatg
    13861 ctcctcggcg atcttggcgc cgacgtgctc ctgatcgaag aagcaggggg cgggtcgtcc
    13921 cgccgcgccg gctccatgcc cggccgcgcc ggcggatccg aggcggctga gcgggccgcc
    13981 gcatataacg cgctcggccg caacaagcgc agcatcgggc tgaacctcaa gaatgagcag
    14041 gcccgtcaag tcttttacga cctctgcagg gacgcggacg tggtgctgga gggctttcgt
    14101 ccaggggttg tcaagcgact gggtgtcgat tatgaaacca tttcggccat taacccccgc
    14161 atcgtctact gctcgctttc gggatacggc cagactgggc cctactccca ggtggtcgga
    14221 cacgacatca actacatctc gatcgccggc gcactcggcg cgattggctg gccgaacact
    14281 cctccggcga tcccgctaaa cctactcgct gatttcgccg ggggcggcct gcaggccgct
    14341 ttcgcaatcg tggtcgctct gctcgcgcgc aatcggattg gccacggcca gtacgtagac
    14401 attgcgatgt cggacggagt catctccctc ctcgcgtcgg ccgccagcgg cttcttcggc
    14461 agcggccttg tgccgcgacg agggcagcac accctgaacg gcgccgtccc ctatttcaac
    14521 gtctacgaaa cggcagacgg caaatggttc agcattgggg cgatcgaacc gtggttctac
    14581 gccaatctgt gcaaagttct cggctgtgag cagtacacgg attaccaaca tgaccagtca
    14641 aaatacgccg agatcaaggc gtatttcagc gagcagttca agaccaggac ccgcgacgag
    14701 tggttccgcg tcctcaccga agtcgacatc tgcgccgcgc ctgtctattc actggacgag
    14761 gtcttcgaag acccgcacaa caggttccgg cagatgcagg tcgaagttga acatccgaag
    14821 ttcggtaagg ttaagcaggt ggggatcggc acgaaattct ctgaaacccc gggaaaagtg
    14881 cgaagcacag cgccgatgaa cggtcagcat accgacgaag ttctacgcgg cttgggaatg
    14941 gacgaagacc gcatcactcg tctgcgcgcg gaaggcggta ttgcgtgagt gtgcttacat
    15001 gtctgccaag cggaacgtgg caatcaggtt aaggaaccaa agggagtaac gaaactgtaa
    15061 aaacggagga aagggcactt caaccgttct accagcccca gatttcctct gagagccgct
    15121 ctcagcgggc ttttgccggt tggacttagc ccgaggctcg cccgtatcct tggcaagtcg
    15181 tgccatcgag accgctttgc cgcttaggct agcggtctct tagtgaccag tatttgtatc
    15241 ggaggcgaac ttagcagaca agaccatacg caaacaatta gtcagtgggg gactacatgc
    15301 agaagcacct cgctgtggct ctcgtcgccg cgctaaccat gtcaggcctt accgccggac
    15361 gggcgtttgc cgatcacgag atcgtcgagc agccgggcga cactatgcaa agcaattcag
    15421 aacacggcga tatatccagc ttatccgcgg cttctggacc ggcgactggt ttcacgctgc
    15481 ccattctgat cgggccgagc tctcccgatt caccaccggg aatcggattc tcctccccgg
    15541 aattaccaga tgacgcggta ataccaggac ggccaattca atatgggctc gcgacctggt
    15601 atggcccggg gttcgacggc cacctgacgt attgtggaga cgtttatgac gaagcggctt
    15661 acacagccgc cagcaacacg ttgccgtgcg gggcagtcgt catcgtcacc aaccaggaca
    15721 acggcgcgag cgtgcgcgta cgaatcaccg atcgcggtgg ttttggaagc gccgtcattc
    15781 tggatctgtc ccgcgcggcg tttggaacca tcgcttccaa cgggtcgggc gtgatcgccg
    15841 tcatggtctc acagtaacgt cggacggagg caattgaggg accaacaagg cgcggtccgg
    15901 gcgttgtcgc cgcgaccgcg ccttgttttc tgcgaccgtt cgtaatcgcg gctggcgccg
    15961 ccgatcagcg gaaacccgag agtgccttcg taacatccga cagaatcgag gccggcgctc
    16021 ggaacaggaa gatgcccgga gcaaacgcat cgccgctctg cacaaagagc gacaaaagga
    16081 cgcaaaacac ggcgaagccc aaaaacgtcg ccgcgatgac gtgcccgggc tgagcctcga
    16141 ccgaaacctg gatgccaatg tccatgagca ggaacatgag cgcgaaggac gcgagaccga
    16201 tgccgaggtt cacgcccggg atgaacatga tcaggctcag ggcaaccggt atggtggcaa
    16261 gactgcacgc ccggatcatt cgctcaacat tcgccgtata ccggaagacg tacacgagga
    16321 ccccgtatgc gacgagaacc cagacgaccc agaagccgaa tgcgatcaat gaccctaata
    16381 acgccgactg ccagaaaact ttcgctttat cgccgtagcc ttgcgtcagc caccaaatcc
    16441 agccgccgag gccgcacaag aaaaacgata tgacggcaat gaccagcgct gggatagttc
    16501 cggccagatc gtctctggct tcttcaaata cggttgtatc gagacgtacg agcctgacca
    16561 gtcggtctac gataacttgt ggatccatc
//
LOCUS       DATCPE010000012         4311 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U7219
            k141_357650, whole genome shotgun sequence.
ACCESSION   DATCPE010000012 DATCPE010000000
VERSION     DATCPE010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955033
            Sequence Read Archive: SRR1586279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 4311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 16:49:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 640
            CDSs (total)                      :: 631
            Genes (coding)                    :: 627
            CDSs (with protein)               :: 627
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4311
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_357650"
                     /isolate="SMAG_U7219"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(119..1168)
                     /gene="pheS"
                     /locus_tag="VIM45_00410"
     CDS             complement(119..1168)
                     /gene="pheS"
                     /locus_tag="VIM45_00410"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEY8819780.1"
                     /translation="MTARTDDLESLRKEALAGLAAADDERALDDWKVEYLGRKSGRLT
                     PIMRSLASLPEDERRRVGSIANAIKSELEAAYENRQASLRHRGLERTIQAGRIDVTLP
                     GRRRDVGRLHPVTQTLRDLLAALGDMGFQVAEGPEVETEYYNFDMLRIPADHPARDMW
                     DTLWLDPLQNGRHPLLLRTHTSPNQIRVMERQEPPIRLAIPGKCYRKEATDATHEWQL
                     TQIEGIAVDENITMADLKGTLAELARRMFGLERQIMLRHSYFPFVEPGVEMAVDCFVC
                     NGAGCSMCKETGWIEILGAGMIHPEVLAGVGYDPERYTGFAWGLGVERVALLRHGIED
                     IRLFYANDLRFLEQF"
     gene            1559..3169
                     /locus_tag="VIM45_00415"
     CDS             1559..3169
                     /locus_tag="VIM45_00415"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEY8819781.1"
                     /translation="MSTTAPEDGAASVTAASPPTPASDAANATGSSVSGPSRTTWRAR
                     WNGWRGDDDTDALVGLVVLGVFIEIFLVVLVARFPLTKFYPGPGVSLGFPQMMSGSWD
                     RDFRWLAIVLAVPFAAFVPALWLARNLRSRAAVAIVFGFAVLFGLTLIRLYPITAADL
                     FHYLADARTLWVYHQNPMQVPPQNHPFVIGISWAQQPSPYGPFWQLLSIIPVMFTGDH
                     WAASIYGLKLLSMAFYLAAGALVFLTVRRTWPGRELLATMVFAWNPFVVFRTIGNGHN
                     DVVMMAFALSAFYFVSRGNWLLALPLLALSVCIKYSTALIVPPVLLYAWMTAGRENRR
                     ELIQGAGIAAAVTLLVFVPFWRGFDTFKTFVQNANMTITSVPQLVSLELQPARAPEDA
                     DHLVRQIGFLLFFLTYAGMLAGLYFRPSFRTLIAVSSLAFIGYLVLDTWWFRPWYFIW
                     FLSLSALLPSFWWTALAVAASFGATFFDLIEQYRSHWDWVWSDSFRGYAAPVVAAFLP
                     LLLLLVLGLAISGAWTMLRDPVRHESSA"
     gene            3244..3495
                     /locus_tag="VIM45_00420"
     CDS             3244..3495
                     /locus_tag="VIM45_00420"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HEY8819782.1"
                     /translation="MAIRGYVLIETEVGSARSVGEQMQRLQHKDATLVAVDTVTGPFD
                     VIVQLEAPDLDKLGNCITEGIQGVPGVKRTTTCLAVRLG"
     gene            3509..>4311
                     /locus_tag="VIM45_00425"
     CDS             3509..>4311
                     /locus_tag="VIM45_00425"
                     /inference="COORDINATES: protein motif:HMM:NF015418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HEY8819783.1"
                     /translation="MISVEDALERVLSYCKQLEPEEKPVLDALGQVLAEDIVASFNIP
                     PLDNTAMDGYAVIGADTEGASHQSPVRLKVVGELAAGYVYDGLVEPATAVRIMTGAPM
                     PAGTDSIVPFEETDESGMIAPQKTGHLDGSVAVFKAARPGNNIRYSGEDVREGETVLT
                     RGTLLRPAHIGVLASLGRARVTVIRRPVVAILSTGDEILEPGEPRRPGTIYDSNAYSI
                     ASQVKRFGGVPRLLGVARDTIEDITSKVRSGLDADLLITSAGVSRGDFDV"
BASE COUNT          732 a         1355 c         1322 g          902 t
ORIGIN      
        1 tcgcggctgc gctcgatcgc ctctatctcc accgtcgaga gcgtcagccg gcgcgcgaga
       61 tcctcgggcg aaagattgac gtcgacgtat tcgcggagcc atcccagcgg aacgcgcatc
      121 aaaactgctc caggaagcga aggtcgtttg catagaagag gcggatgtcc tcgatgccat
      181 ggcgcagtaa cgcgacacgc tcgacgccga gtccccaggc gaagccggtg tagcgttcgg
      241 gatcgtagcc gacaccagcg aggacctcgg ggtgaatcat gccggcgccg aggatctcga
      301 tccagccggt ctccttgcac atggaacaac cagcgccgtt acagacgaag caatcgactg
      361 ccatctcgac cccgggctcg acgaagggaa aataggaatg gcgcaacatg atctgccgtt
      421 ccagaccaaa catgcgccgc gcgagctcgg ccagcgtgcc tttcagatcg gccatcgtga
      481 tgttctcgtc taccgcgatc ccttcaattt gcgtgagttg ccactcatgg gtcgcatcgg
      541 tcgcttcctt ccggtagcac ttgcccggga tcgccagccg gatcggcggc tcctgccgct
      601 ccatcacgcg gatctggttc ggagaagtat gggttcgcag cagcagcggg tggcggccgt
      661 tttgcagcgg gtccaaccag agggtgtccc acatatcgcg agccgggtgg tcggcgggga
      721 tgcgcagcat gtcgaagttg tagtactcgg tttcgacctc cggcccttcc gcgacctgaa
      781 agcccatgtc cccgagcgcg gccaacaggt cccgcagcgt ctgggtcacg gggtgcaacc
      841 ggccgacgtc gcgccgccgg ccgggcagcg tcacgtcgat ccgtccagcc tgaatcgtgc
      901 gctccaagcc gcgatgtcgc agagaggcct ggcggttttc gtacgccgcc tcgagctcgc
      961 ttttgatggc gttcgcgatc gaaccgaccc gccgccgttc gtcctccggc aacgacgcca
     1021 gtgaccgcat gatcggcgta aggcggccgg acttgcggcc caagtactcg actttccagt
     1081 cgtcgagggc gcgctcgtcg tcggccgctg ccagcccggc cagcgcctct ttgcggagcg
     1141 attccagatc gtcggttcta gccgtcataa ccggtctccg ggtctatgtt tcgctcttcg
     1201 ccgggagaac agcggccggg acgcacgcgc cccggccgcc cgccatcgct cttggcgtct
     1261 attgtaggga agccaatcag gcgcgatcaa cgcggacccc tatctctccc gacctcgctt
     1321 tccctcagga ggacacgctt cgctaccccg atgcagggaa agaaaggaga ttgggcgtgg
     1381 cacgattgct ggtaacaagc gttgatgaat agcgaggcaa ggcacgcaaa gcgtgccgga
     1441 gtcattcaca aagccgcgta ttatccggcc aacgcccgct gcggcaccgc aggatcgccc
     1501 ctttccctgc atcggggaaa ggggaggggg ataggggttg gacgcgtata ctggcagcat
     1561 gtccaccacc gcgcccgaag acggcgccgc cagcgtgacc gccgcctcac cacctacgcc
     1621 ggcatctgat gcggcgaatg ccacgggctc atccgtctcc ggtcccagcc gtaccacctg
     1681 gcgggctcgt tggaacgggt ggcgcggcga cgacgacacc gacgccctcg tagggctggt
     1741 cgtgctcggc gttttcatcg agattttcct tgtagtgctc gtcgctcgtt tcccgttgac
     1801 caagttctac ccgggcccgg gcgtgtctct cggcttcccg cagatgatgt ccggctcctg
     1861 ggatcgtgat tttcggtggc tggcgatcgt cctggcggtg ccgttcgccg cattcgtccc
     1921 ggcgttatgg ttggcgcgca acctacgcag ccgcgccgcg gtcgcgatcg tgttcggctt
     1981 tgccgtcctg ttcgggctga cgctcatacg gttatacccg atcaccgccg ccgacctgtt
     2041 tcactacctg gccgacgcac gcacattgtg ggtctaccac cagaacccga tgcaggtgcc
     2101 gccgcaaaat cacccgttcg tcattggcat cagctgggcc cagcagcctt ctccatacgg
     2161 tcctttctgg cagttgttgt cgatcatccc cgtgatgttc accggcgacc actgggcagc
     2221 gtctatttac ggtttgaagc tgctgtccat ggcgttttat ctcgccgctg gtgccctggt
     2281 gttcctcacg gtgcgccgaa cctggcctgg gcgagagctg ctggcaacaa tggtgttcgc
     2341 gtggaatccg tttgtcgttt ttcgcacgat tggtaacggt cacaacgacg tggtgatgat
     2401 ggcgttcgca ctatcggcgt tctacttcgt ttctcgcggc aactggctgt tagcactgcc
     2461 gctgctcgcc ctttcggtgt gcatcaagta ctcaacggcg ctgatcgtgc cgccggtgtt
     2521 gctgtacgca tggatgacgg ctggcagaga aaaccggcgt gagcttatcc agggagcggg
     2581 gatcgcggct gcagttaccc tgctggtgtt tgtgccgttc tggcgaggct ttgacacgtt
     2641 caagaccttc gttcaaaacg cgaacatgac catcacgtct gtgcctcagc ttgtgtcgct
     2701 cgagcttcaa ccggcgcgcg cacccgagga tgcggatcac ctggttcgcc agatcggttt
     2761 tctgttgttc ttcctaacgt acgcgggaat gctcgccggc ctctattttc gcccgtcatt
     2821 tcgaacgctc attgcggtgt ccagcctcgc cttcatcgga tacctggtac tcgatacatg
     2881 gtggttccgt ccctggtact tcatctggtt tctctccctc tccgcgttgc tgccgtcgtt
     2941 ttggtggacg gctctggcgg ttgccgccag ttttggagcg actttcttcg acctcattga
     3001 gcagtatcgg agtcactggg actgggtatg gtcggactcg ttccgcggat atgcggcacc
     3061 ggttgtcgcg gcgtttctgc cgttgttgtt acttttggta ctggggcttg ccattagcgg
     3121 tgcctggact atgttgcgcg atccggtgag gcacgaaagt tccgcttgac gctcaccggc
     3181 gtagcgctat gatcgccgag aaccaatccc gccgttttac aaagtatgtg aacgaggtga
     3241 ccgatggcga ttcggggcta cgtcctcatc gagaccgaag ttggtagcgc ccgatcggtt
     3301 ggcgagcaga tgcagcgcct acagcacaag gacgctacgc tcgtcgcggt cgacacggta
     3361 actggaccgt ttgacgtgat cgtccagttg gaagctcctg acctggacaa gctcggtaac
     3421 tgcatcaccg aaggcattca gggcgtcccc ggcgtcaagc ggaccaccac ttgccttgcg
     3481 gtccgtctcg gataagcggg ttgtggcaat gatcagcgtc gaagacgccc ttgaacgcgt
     3541 tctttcctac tgcaaacagc tcgagccgga agagaagccc gtcctggacg cgctcggcca
     3601 ggttctggct gaagacatcg tcgcgtcgtt caatatcccg ccgctcgata acacggcgat
     3661 ggacggttac gccgtcatcg gggccgacac cgaaggcgca agccatcaat cgccggttag
     3721 gctaaaggtc gttggcgaat tggcggccgg ctacgtctat gacggacttg ttgagccggc
     3781 cactgccgtc cggatcatga ccggagcgcc gatgcccgcc ggcaccgatt cgatcgttcc
     3841 gtttgaggaa acggacgaga gcggcatgat cgcaccccaa aagacgggtc acctcgacgg
     3901 gtcggttgcg gtcttcaaag cggcccggcc gggaaacaac atccgttaca gcggcgaaga
     3961 cgtacgcgaa ggcgagacgg tgttgacccg cggaacgctg ctacggcctg ctcacattgg
     4021 agtgcttgcc tcacttggtc gggcacgcgt gacggtcatt cgacggccgg tggtggccat
     4081 cctctcgacg ggcgatgaga tcctggagcc gggtgaaccc cgccgacccg gaaccattta
     4141 cgatagcaac gcttacagca tcgcctccca ggttaaacgc ttcggcggcg tgccgcgcct
     4201 gctcggcgtc gcccgcgaca ccatcgagga catcacatca aaggtccgtt ccggactcga
     4261 cgcggatctc ctcattactt cggccggtgt ctcgcgcggc gactttgacg t
//
LOCUS       DATCPE010000013         3295 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U7219
            k141_293273, whole genome shotgun sequence.
ACCESSION   DATCPE010000013 DATCPE010000000
VERSION     DATCPE010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955033
            Sequence Read Archive: SRR1586279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 16:49:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 640
            CDSs (total)                      :: 631
            Genes (coding)                    :: 627
            CDSs (with protein)               :: 627
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3295
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_293273"
                     /isolate="SMAG_U7219"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1053
                     /locus_tag="VIM45_00430"
     CDS             <1..1053
                     /locus_tag="VIM45_00430"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009471850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEY8819784.1"
                     /translation="MDLKRGKPMADDTIFRIYSMTKPITSVALMQLYEHGHFQLNDPV
                     HRVIPEWRDQRVYVSGQGAAMETRALDRPVTFRQVLSHTGGLTYGATDHPVDKVYREL
                     GVRRGEGETLRTFVEKLGRVPLRYTPGEHWMYSLSTDVCGYLVEAISGQRFDRYLQEH
                     IFGPLRMRDTSFRVAAEKVDRFAANYQRQPDKTLQLVDDSATSTYLKEPSFFSGGGGL
                     TSTTADYFRFCEMLRRGGELDGVRILGPRTVQLMTKNHLAGGRDLTQMALGAFSETAN
                     EGVGFGLGFAMTLSEVDTGALGEGDYYWGGAASTIFWIDPAEDMVVIFMTQLMPSATF
                     NFRGQLKNIIYSAIVE"
     gene            1063..2127
                     /locus_tag="VIM45_00435"
     CDS             1063..2127
                     /locus_tag="VIM45_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006574594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEY8819785.1"
                     /translation="MNPTVTVTPKRLFEFLLNVALVRPIFIWGAPGIGKSSIVQQFAA
                     DVGLPCVSLLGSQLAPEDLIGVPQIFEGTSRFCPPSMIARPDPYCLFLDELNACSHEV
                     QKAFYSLIHERRIGEYFLPKGSIVIGAGNRAQDSAIVKPMSSALLNRMVHVHLAVSHR
                     DWLEWAEANAIHHLVVEYIKARPDHLWSQPPKHEEPFSTPRTWHMLSDALHAYGERLT
                     NDELEVLAYGCLSPQHAGQFKAFIKQLRGGFRLAGILKGDAGWPSAPEDRDILYFLAQ
                     SFRAQLIKELPADRAALTPIHRETAHRAKSLLTDLATINLEMAQLAVARDDDGCTLPD
                     WLMVEIVRDLPRLVESARGA"
     gene            2117..>3295
                     /locus_tag="VIM45_00440"
     CDS             2117..>3295
                     /locus_tag="VIM45_00440"
                     /inference="COORDINATES: protein motif:HMM:NF024601.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEY8819786.1"
                     /translation="MARKTAVTAALDVAADNFRRGHIALRAHPLFAPLTAGAYIIRQN
                     GNLCPEGAWAVVSADGQIHVHPRRRGQPEEWVYVLAHCLLHLGLGQFREHQRPLEWNA
                     ACDVAVTRFLAALKLGRAPEGFIPIEDPAVRDEELLYERFCRLGLPPGYSPDMRLTEP
                     PASRGTRRIDWQAALANGVSAAVMSAVNVAGGREAYLGARRKIGTAAARAREWFINSY
                     PLLGALAVTFRLVEDAAVCRRLGISVAAVDAESQEIFVNPAAALTEGECRFVIAHELL
                     HVGLRHDARRQGRDPFFWNLACDYVINGWLVEMRVGDLPKIGVLYDAALKGESSETIY
                     DQITTDLRRYRKLATLRGQGLGDMLERGDHSWWNAGEGVGLDELYRRYLGQGLAYHET
                     E"
BASE COUNT          578 a         1039 c         1070 g          608 t
ORIGIN      
        1 atggacctga agcgtggcaa gccgatggcg gatgacacca tctttcgcat ctactcgatg
       61 acgaagccga tcacctcggt cgcgttgatg cagctgtacg agcacggcca ttttcaattg
      121 aacgatccgg tgcatcgggt catcccggag tggcgtgatc agagagtgta tgtgtcgggc
      181 cagggcgcgg caatggaaac gcgcgcgctg gatcggccgg tgacgtttcg ccaggtgctg
      241 agccataccg gcggtctgac ctacggcgcc acggaccacc cggtcgacaa ggtataccgc
      301 gagctgggcg tacgacgcgg cgaaggcgaa acgctcagga cgttcgtcga gaaactggga
      361 cgtgtgccgc tgcgctatac gcccggagag cactggatgt attcgctatc gaccgatgtc
      421 tgcgggtacc tggtcgaggc gatctccggg cagcggttcg accggtattt gcaggagcac
      481 atcttcggcc cgctgcggat gagggatacg tcgttccggg tggcagcgga gaaggtcgat
      541 cgcttcgccg ccaactacca acgccagccg gacaagaccc tgcagttggt cgacgactcg
      601 gccaccagca cctacctcaa agagccgtct ttcttctcag gcggcggcgg gctgaccagc
      661 accaccgcgg actacttccg cttctgtgag atgctgcgcc gtggcggcga gttggacggt
      721 gttcgcatcc tcgggccgcg cacggtccag ctgatgacca agaaccacct ggccggcggt
      781 cgcgacctga cgcagatggc cctcggagcg ttctcggaga cggccaatga aggcgtcggc
      841 ttcgggttgg gcttcgccat gacgctcagt gaggtcgata caggcgccct cggcgaaggg
      901 gactattact ggggcggcgc ggcatcgacg atcttctgga tcgatccggc ggaagatatg
      961 gtggtgatct tcatgacgca gctaatgccg tcggcgacgt tcaatttccg cggtcagctg
     1021 aagaacatca tctattcggc gatcgtggaa tagtggattg gtatgaatcc aacggtgact
     1081 gtgacgccga agcggctgtt cgagttcttg ctgaacgttg cgctggtgag gccgatattc
     1141 atctggggcg cgccggggat cggcaagtca tcgatcgtcc agcagtttgc ggccgacgtc
     1201 gggttgccgt gtgtctctct gctcggcagc cagctggcac ctgaggacct gatcggcgtg
     1261 ccccagatct ttgaggggac gagccgcttc tgcccgccca gcatgattgc gcgccccgac
     1321 ccttactgtc tgttcctcga cgagctcaac gcctgttccc atgaagtgca aaaggcgttc
     1381 tacagcctga tccacgaacg gcggatcggc gagtacttcc tgccgaaggg ctcgattgtg
     1441 atcggcgccg gcaaccgcgc ccaggacagc gcaatcgtca agccgatgtc gtcggcactg
     1501 ctcaatcgca tggtgcacgt ccacctggcg gtgtcgcacc gcgactggct ggagtgggcc
     1561 gaggccaacg ccattcacca cctggtggtc gagtacatca aagcgcgacc agaccacctg
     1621 tggagtcagc cccccaagca cgaggaaccg ttctcgacgc cgcgcacctg gcacatgctc
     1681 agcgatgcgc tccacgccta cggtgaacgg cttaccaacg acgagctcga ggtgctcgcc
     1741 tacggctgcc tttcgccgca gcatgcgggg cagttcaagg cgttcatcaa gcagcttcgc
     1801 ggcggtttcc ggctcgcggg gatcctcaag ggcgacgcgg gttggccgtc tgctccggaa
     1861 gaccgcgaca tcctctactt tctggcccag tcgttccgcg cccaactgat caaggagctg
     1921 ccagccgacc gtgccgcact gacgcccatc catcgcgaga ctgcgcatcg cgccaagtcg
     1981 ttgcttaccg acctcgccac gatcaatctc gagatggcac aactggcggt tgcgcgggac
     2041 gacgacggtt gcacgctgcc tgactggctg atggtcgaga tcgtgcgtga cctgccgcgc
     2101 ctggtagaga gcgcccgtgg cgcgtaagac cgccgtgact gcggcgctcg acgtcgccgc
     2161 tgataacttc cgcaggggcc acatcgcgct gcgcgcacac ccgctgtttg ctcccttgac
     2221 cgccggagcc tacatcatcc gtcagaatgg caacctctgc ccggagggcg cctgggccgt
     2281 cgtttcggcg gacggccaaa tccacgtgca cccaaggcgg cgcgggcagc cagaagagtg
     2341 ggtgtacgtc cttgctcact gcctgcttca cctcggtctc ggccagttcc gggaacatca
     2401 gcgtccactg gaatggaacg ccgcctgtga tgtggcggtg acgcgctttc tggccgcgct
     2461 gaagctgggt cgggcgcccg agggctttat cccgatagag gatccggcgg tacgcgatga
     2521 agaactcttg tacgagcgct tctgtcgcct cggtttgccg ccgggatact cccccgatat
     2581 gcgcttgacg gagccgcctg ccagtcgcgg taccaggaga attgactggc aagcagcgct
     2641 cgctaacggc gtgtccgccg ctgtgatgag cgcggtgaat gtcgcgggcg gacgcgaggc
     2701 gtatctcggc gcccgccgca agatcggaac ggccgccgcg cgggcgcggg aatggttcat
     2761 caacagttat ccgttgctcg gcgccctggc ggtgacgttc cgcctggtcg aagatgcagc
     2821 cgtctgccgg cgcctcggga tatcggtcgc agcggtcgat gcagagagcc aggagatctt
     2881 cgtcaacccg gccgccgcgc ttacggaagg cgaatgccgt ttcgtgatcg cgcacgagct
     2941 cctgcacgtc gggctgcgcc acgatgcacg ccgtcaggga agggatccat tcttctggaa
     3001 cctcgcctgc gattacgtca tcaacggctg gctggtggag atgcgtgtcg gcgacctacc
     3061 gaagatcggc gtgctttacg acgccgccct caagggcgaa tcgtccgaga ctatctatga
     3121 ccagatcacc accgaccttc gccgctaccg caagctcgcc accttgcgcg gccaagggct
     3181 cggcgacatg ctggagcgtg gcgaccactc gtggtggaac gcgggcgaag gcgtcgggct
     3241 cgatgagctc taccgtcgct atctcggcca ggggctcgcc taccacgaga ccgag
//
LOCUS       DATCPE010000014         5090 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U7219
            k141_260998, whole genome shotgun sequence.
ACCESSION   DATCPE010000014 DATCPE010000000
VERSION     DATCPE010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955033
            Sequence Read Archive: SRR1586279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 5090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 16:49:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 640
            CDSs (total)                      :: 631
            Genes (coding)                    :: 627
            CDSs (with protein)               :: 627
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5090
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260998"
                     /isolate="SMAG_U7219"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /gene="upp"
                     /locus_tag="VIM45_00445"
     CDS             <1..447
                     /gene="upp"
                     /locus_tag="VIM45_00445"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014435260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEY8819787.1"
                     /translation="ATTGNQLADRIALVPILRAGISMADGVLQMVPLAQVWHLGMYRD
                     HETLQPVAYYNKLPRPPEFDLALVLDPMLATGGSAVAAISVLKDAGIERIKFIGLIGA
                     PEGVAHVHELYPEVPIHLAALDDRLDEHSYIVPGLGDAGDRQFFTL"
     gene            complement(470..1594)
                     /locus_tag="VIM45_00450"
     CDS             complement(470..1594)
                     /locus_tag="VIM45_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019916534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - intracellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HEY8819788.1"
                     /translation="MNPHDPRQKDDIRRWQSNLKSEWEASALYQALANAEKDPVKANV
                     FRKLAEVEDRHASRWEGFLRASGAPLPSAGRTLRGRVLHALAGRFGARAVLHMVVANE
                     LAGVTMYDDQADSGNLPREERGHARIFSAMTGGRTGADIAASESWHRRDSGGGLRAAV
                     FGVNDGLVSNLSLVFGVAGANPAHRFILLAGLAGLLGGSFSMAAGEFISVTTQRELFE
                     RQIALEKDELENQPEEEAEELSLIYQAKGIPEQDAERLAHRIIGDKAVALDTLAREEL
                     GLDPDSLGSPWQVAGASGLSFGSGAIVPVIAYFFSLSRWTSLGVSAVLSVLALIAVGL
                     GISVVTGKQPVISAARMVLVGSIAAAVTITIGHIIGVTTS"
     gene            1713..2399
                     /locus_tag="VIM45_00455"
     CDS             1713..2399
                     /locus_tag="VIM45_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY8819789.1"
                     /translation="MAHILVVEDERRLASLMKRVLEEEGHVVDVANDGDEGLTLGTAD
                     GFDAIVLDVLLPGINGFDVCRQVRKEGVQTPVLMLTARGSVEDRVTGLDAGADDYLVK
                     PFAFEELLARIRALTRRAPAITTTSQLCIADLTMDLTLHEVRRGGRSVTLTGKEYALL
                     EYFLRHPNQVLTRAQILDHVWGYDSEPGSSVVEIYIHYLRNKVDRNYGQTLIQTVRGV
                     GYMLRVPEKP"
     gene            2396..3724
                     /locus_tag="VIM45_00460"
     CDS             2396..3724
                     /locus_tag="VIM45_00460"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8819790.1"
                     /translation="MTSLDRAARLARRVARPSTPAALTYLQRARIRLTAWYVGILALL
                     FVIAGIAVYAVLSQVLSHEIDNDLRAVASSVANRDVPALTEQEQNGFGSPYPDVFALY
                     LDQHGILNYPKVIPFPELPDAAGINAAARSGNPDLRTVTKSRQTIRLLSQLIRQNGQV
                     IGTVQVGKPLRTYERDMHALALVLLGSGMAALLLATVGGLVVANRALRPVQQSWHRQQ
                     AFVADASHELRTPLAIVRADAEVLLRAANRPVAENRDLVEDIVKETDHLSGLVTDMLT
                     LTRLDAGRLPLEREEFNASELLEDVAAQTRRLLLDRRVDVKIEAPAVLSVTADRERIL
                     QVLRILVDNSQRYTPDGGTIGLGARRSGNGAVLSVVDTGAGIAAKDMGHVFDRFYRAD
                     RSRARTIGGAGLGLAIARGIVEAHGGTLQIDSSPGRGTAVNVELPAGGSR"
     gene            3836..4060
                     /locus_tag="VIM45_00465"
     CDS             3836..4060
                     /locus_tag="VIM45_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8819791.1"
                     /translation="MASDSTSVQEVRKQLSAHAHQVYGTYRARDLATQINQTASAMAA
                     VGGYPLATETEPWTASDAPVIRREDGRIRA"
     gene            4057..>5090
                     /locus_tag="VIM45_00470"
     CDS             4057..>5090
                     /locus_tag="VIM45_00470"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEY8819792.1"
                     /translation="MIESRPLHRLNASAIAHMVASGDLAVDEIARQTLARIRELDASI
                     HAWICLEPEAVLAEAATLHAEARAGNLRGPLHGVPVGIKDIYDVAGMPTRAGSNVLAD
                     AGPAARDSAPVAMLRRAGALIVGKTATTEFASADPARTRNPWNLEHTPGGSSSGSAAA
                     VAAGMVPAAMGSQTAGSILRPASFCGVVGFKPSFGRIPRDGVLPFAWTLDTMGPLTRT
                     VADARLLLRSLTPSTWSLPDLETSQFGSHGPRVGFVPRLFQDRLDRSMRDMLSAAATR
                     LQSAGAIVDEVTLPADFETVLHAQHLIMITEAAAFHQRAFPGKLNLYGPRIRRMVEIG
                     ALVPATSYLNA"
BASE COUNT          933 a         1660 c         1587 g          910 t
ORIGIN      
        1 gcgacaaccg gcaaccagct agccgaccgc atcgccctcg tccccatcct tcgtgccgga
       61 atcagtatgg cggacggagt cttgcagatg gtgcccctcg cacaggtctg gcacctgggc
      121 atgtaccgcg accatgaaac gctgcaaccg gtcgcgtact acaacaagct accccggccg
      181 ccggagttcg acctcgcgct cgtgttggac cccatgctgg cgacaggcgg ctccgccgtc
      241 gcggcgatca gcgttctgaa agacgccggt atcgaacgca taaagttcat cggcttgatt
      301 ggggcgcccg agggcgtcgc gcacgtccac gaactctacc cggaggtgcc tatccacctc
      361 gccgcgctcg acgaccgcct cgacgagcac agctatatcg tcccagggct cggcgatgcc
      421 ggcgacaggc agtttttcac gctgtgaggc tgtttgcgct ccagaggggc taggaagttg
      481 ttacgccgat gatatggccg atcgtgatcg tgacggcggc ggctatggag ccaaccagga
      541 ccatgcgcgc cgcgctgatg accggctgtt tgcccgtcac cacggagatg ccgagtccaa
      601 ccgcaatcaa tgccagcacg ctgagcacgg cgctgacgcc gagcgaagtc cagcgcgaca
      661 ggctgaagaa ataggcgatg acggggacga tcgcaccgga accgaacgac agccccgatg
      721 caccggccac ctgccacggt gagcccaggg agtcgggatc gagacccagc tcttcgcggg
      781 ccagagtatc gagcgcgact gccttatcgc caatgatgcg gtgggcgagc cgctctgcat
      841 cttgttccgg tatccccttg gcctggtaga tcagcgatag ctcttcggcc tcttcttccg
      901 gctggttttc aagctcgtcc ttctccaggg ctatctgccg ctcgaacaac tcacgctgcg
      961 tcgtcaccga aatgaattcg cccgcggcca tggaaaatga accgccgagc agaccggcga
     1021 gcccggccag taggatgaat cggtgggcgg gattggctcc ggccacgcca aagacgaggc
     1081 tcaagttgga caccaggccg tcgttcacac cgaaaacggc ggcgcgcagg ccgccccccg
     1141 aatctctgcg gtgccacgat tctgaggcgg cgatgtcggc accggttcgc ccgcccgtca
     1201 tcgccgaaaa tatgcgcgcg tgaccgcgtt cctcgcgcgg aagatttccg gaatccgcct
     1261 gatcgtcgta catggttacg ccggcaagct cattggccac caccatgtgc aacacggccc
     1321 tggcgccaaa ccgccccgcc agcgcatgca aaaccctccc gcgcagcgtt cggcccgccg
     1381 acggcagagg ggcacccgac gcccgcaaaa agccctccca gcgtgaggca tgccggtctt
     1441 caacttctgc cagcttccgg aaaacgttcg ccttgactgg atccttttcg gcgttggcga
     1501 gcgcttggta cagcgccgac gcttcccact cgcttttgag gttcgactgc cagcggcgaa
     1561 tgtcgtcctt ttgtcgcggg tcatgcggat tcattcgggc ggtctccttg cgatcgagca
     1621 cggccagagg ctgaaaactt tggcagagca ggtccggcga ctacaattct acaggagcgc
     1681 ggctgtacac gttgccgcga gcgcggggat cgatggctca cattttggtg gtcgaggacg
     1741 aacggcggtt agccagcctc atgaagcgcg tcctcgaaga agaggggcat gtcgtcgacg
     1801 tcgccaacga cggtgacgaa gggctcacac tcgggacagc ggacggcttc gacgcgatcg
     1861 tgctcgacgt tctcttaccg ggaattaacg ggttcgacgt ctgccgccag gtacgaaagg
     1921 aaggggtcca gacgccggtg cttatgctga ccgcgcgcgg ctcggtcgaa gatcgcgtca
     1981 ctggactaga cgcgggcgcg gacgactacc tggttaagcc gttcgccttc gaagagttgc
     2041 tagcccgaat acgcgcgttg acccgccgtg cacccgcgat cacgacgaca agtcagctct
     2101 gcatcgctga tctgacgatg gacttgacgc tgcacgaagt ccggcgtggg ggcaggtcgg
     2161 ttacgctcac tggcaaagaa tacgcgctgc tcgagtactt cctgcgtcat ccaaaccagg
     2221 tgctcacccg agcgcagatc ctggaccacg tttggggcta cgactccgag cccgggagct
     2281 cagtagtgga gatctacatc cattacttgc gcaacaaggt cgaccgaaac tatggccaga
     2341 cgctgataca gaccgtccgc ggcgtgggtt acatgctgcg cgttccggag aaaccttgac
     2401 gtcgctcgac cgcgctgccc gactcgcccg tcgcgtagcg cgaccatcca cgcccgcagc
     2461 gctcacctac ttgcagcggg cgcggatacg gctgactgcc tggtatgtcg gcatcctggc
     2521 gctgctgttt gtcattgcgg gaatcgctgt ttatgcggtc ctctcgcagg tgcttagcca
     2581 cgagatcgac aatgacctgc gcgctgttgc ctcgtccgtg gccaaccggg atgttccggc
     2641 gctgaccgaa caagagcaaa acgggttcgg cagtccctac cccgacgtgt tcgcgctcta
     2701 tctcgaccag cacggtattc tgaactaccc gaaggtcata ccgtttcccg agctacccga
     2761 tgccgctgga atcaatgccg cggcacggtc gggcaatccc gatctgcgaa cggttaccaa
     2821 gtcgaggcaa acgatacgcc tgctctcgca attgatccgc cagaacggtc aggtaattgg
     2881 caccgtccaa gttggtaaac cgctgcgcac ctacgagcgc gatatgcacg cgcttgcgct
     2941 tgtattgctg ggcagcggca tggcggcgct cttgctcgcg actgtaggcg gcctcgtggt
     3001 cgccaatcgg gcgcttcgtc cggtccaaca gagctggcac cgtcaacaag cgtttgtcgc
     3061 cgatgcctcg cacgagctgc ggacgccgtt ggcaatcgtc cgcgccgacg ccgaagtgct
     3121 gctccgcgcg gccaaccggc cggtggcaga gaaccgtgac ctggtcgagg atatcgtcaa
     3181 agaaaccgat cacctttccg gtttggttac cgacatgctg acgcttaccc gtctggacgc
     3241 cggccggctg ccgctggaac gagaagaatt caacgcgtcc gagctcttgg aggacgtggc
     3301 cgcgcagacg cggcggctgc tgctcgatcg ccgcgtcgac gtgaaaattg aagcgccggc
     3361 cgtgctgtcg gtgactgcag accgggagcg aattctgcag gtccttcgca ttcttgtaga
     3421 caactcacaa cgttatacgc cggacggcgg caccataggc cttggggcgc ggcgcagcgg
     3481 caatggcgct gttctgagcg tcgtcgacac gggagccgga atcgccgcca aagacatggg
     3541 gcacgtgttc gaccggtttt atcgcgccga tcgatcgcgg gcacgcacga ttggcggtgc
     3601 ggggcttggc cttgcgatcg cccgcggtat cgtcgaagct cacggcggaa ccctgcagat
     3661 cgatagctcg cccggccgcg gaacagcggt caacgtcgaa ctgcccgccg gtggaagccg
     3721 ctgatctgac cgactgccgg cgtgctaccg cagtaactga ttctgctgca cggctagcgc
     3781 gccccttccg accgccgtac acttgacgcc gtctacgacg atccggaggc gtcatatggc
     3841 gtccgattcc accagcgtcc aggaggtgcg gaaacagctc agcgcccacg cgcaccaggt
     3901 gtatggcacc tacagggccc gtgacctcgc cacccagatc aaccagaccg cgtcggctat
     3961 ggcggcggtc gggggatatc ccctggcgac ggaaaccgag ccgtggaccg cctctgatgc
     4021 acccgttatc cgacgtgaag acggtcgcat tcgagcatga tcgaatcgcg tccgctccat
     4081 cgcttgaacg catcggcaat cgcccacatg gtcgcctccg gggatctggc ggttgacgag
     4141 atcgcgcgcc agacgttggc gcggatccgc gagctcgacg catccatcca cgcctggata
     4201 tgcctcgaac ccgaagctgt actcgccgaa gcggcgacgc tccacgccga ggcgcgggca
     4261 ggcaacctgc gcgggccact gcacggcgtt ccagtcggca tcaaggacat ctacgacgtt
     4321 gccggtatgc cgaccagagc aggatcaaac gtcctcgcgg acgcggggcc ggctgcgcga
     4381 gactcggcgc cggtcgcaat gttgcgccgc gcgggggcac tgatcgtcgg caaaacggcg
     4441 acgacggagt tcgcgagcgc cgacccggca cgaacgcgaa atccgtggaa tctggagcac
     4501 actcccggcg gttccagcag cggctcggct gcggccgtgg ccgcgggcat ggtgccggcg
     4561 gcaatgggct cgcaaacggc tggctcaatt ctgcgcccag cttccttctg tggcgtggtc
     4621 gggttcaagc ctagctttgg ccgcattccg agagacggcg tgttgccgtt tgcctggacg
     4681 ctggacacga tggggccgct gacgcgcacc gtcgcagacg cgcggctcct cttgcgctca
     4741 ctgacgcctt ctacatggtc attacccgat ctggaaacgt cccaattcgg ctcgcatggt
     4801 ccgcgggtcg ggttcgttcc ccgcttgttt caggaccgtc tcgatcgcag catgcgagac
     4861 atgctctccg cggcggcgac gcgattgcaa tcggccggcg ccattgtgga cgaagtcacg
     4921 ctaccggccg acttcgagac cgtgctccac gctcaacacc tgatcatgat taccgaagcg
     4981 gctgcgtttc atcagcgggc cttccccggc aaactgaacc tctacggccc acgtattcgt
     5041 cggatggtcg aaatcggggc gctggtgccg gcaacgtcgt atctgaatgc
//
LOCUS       DATCPE010000015        13047 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U7219
            k141_585451, whole genome shotgun sequence.
ACCESSION   DATCPE010000015 DATCPE010000000
VERSION     DATCPE010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955033
            Sequence Read Archive: SRR1586279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 13047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 16:49:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 640
            CDSs (total)                      :: 631
            Genes (coding)                    :: 627
            CDSs (with protein)               :: 627
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13047
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_585451"
                     /isolate="SMAG_U7219"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..117
                     /gene="rplE"
                     /locus_tag="VIM45_00475"
     CDS             <1..117
                     /gene="rplE"
                     /locus_tag="VIM45_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514647.1"
                     /note="part of 50S and 5S/L5/L18/L25 subcomplex; contacts
                     5S rRNA and P site tRNA; forms a bridge to the 30S subunit
                     in the ribosome by binding to S13; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEY8819793.1"
                     /translation="YDRIDRIRGMEITMVTSARTDEEGRRLLQLLGMPFSKS"
     gene            135..320
                     /locus_tag="VIM45_00480"
     CDS             135..320
                     /locus_tag="VIM45_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002557082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HEY8819794.1"
                     /translation="MAKVSQIVRSTRKPKFSTRQKNRCKLCGRPRGYIRKFAMCRICF
                     RGLAHVGQIPGVTKSSW"
     gene            438..854
                     /gene="rpsH"
                     /locus_tag="VIM45_00485"
     CDS             438..854
                     /gene="rpsH"
                     /locus_tag="VIM45_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEY8819795.1"
                     /translation="MNMTDPIADMLTRIRNAVASRHDTVTMPASKVKAEIARVLKDEH
                     FIRDFELVEESDRRGHGPHKVLRVHLSYSGKREAILSGLQRVSKPGLRVYVQKKEIPR
                     VYGGLGIAILSTPQGIMTGHQAWRQQIGGEVLCYVW"
     gene            905..1459
                     /gene="rplF"
                     /locus_tag="VIM45_00490"
     CDS             905..1459
                     /gene="rplF"
                     /locus_tag="VIM45_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEY8819796.1"
                     /translation="MSRIGRQPVPVGQGVNVMIDGKVVTVKGPKGQLRREFSSEVTIE
                     QRDGTLVVARQDDEPRHRALHGLSRSLLANMVEGVSNGFTRVLEVQGVGYRIAASANG
                     ITMSLGFSHGVEVSAPPGITFAVEGTNRVRIEGIDKELVGQVAANIRAIRKPEPYKGK
                     GIRYQGENVRRKAGKAGKGAKGAK"
     gene            1505..1876
                     /gene="rplR"
                     /locus_tag="VIM45_00495"
     CDS             1505..1876
                     /gene="rplR"
                     /locus_tag="VIM45_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEY8819797.1"
                     /translation="MVSKVKTSRAQRLRRHRRVRKRVAGTEQRPRLAVFRSLAHIYAQ
                     VIDDTSGCTLVAASDRDADVAPEAKGKPKTQRAELVGVAIARKATAAGLKLVVFDRGG
                     FLYHGRIKALAEAARKSGLSF"
     gene            1891..2331
                     /gene="rpsE"
                     /locus_tag="VIM45_00500"
                     /pseudo
     CDS             1891..2331
                     /gene="rpsE"
                     /locus_tag="VIM45_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019535607.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
     gene            3162..3338
                     /gene="rpmD"
                     /locus_tag="VIM45_00505"
     CDS             3162..3338
                     /gene="rpmD"
                     /locus_tag="VIM45_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEY8819798.1"
                     /translation="MATLRITWKKSAIGYAKDQKLTIQSLGFRRLGQTVEQEDTRSTR
                     GMIHKVRHLVIVEE"
     gene            3367..3831
                     /gene="rplO"
                     /locus_tag="VIM45_00510"
     CDS             3367..3831
                     /gene="rplO"
                     /locus_tag="VIM45_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011197171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEY8819799.1"
                     /translation="MKATPGARHKRIRVGRGNGSGKGTYSGKGLKGQKARSGSGLLPG
                     FEGGQFPLIRRMARKRGFTNFARIEYEWVNLDQLAELPSGTVVNQESLLAAGIIKNIK
                     RPLKVLGDGEMTGALMVQAHKFSASARQKIEAAGGTVEEVAGGTASNVIRGE"
     gene            3878..5140
                     /gene="secY"
                     /locus_tag="VIM45_00515"
     CDS             3878..5140
                     /gene="secY"
                     /locus_tag="VIM45_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEY8819800.1"
                     /translation="MREKLLFTFAMLVVFRLLTHVPLPNVDQNALTSAFKGNAVLGFL
                     NVFSGNALRKLSVAALGVYPYITASIVMQILVPVIPRLQALSKEGEHGRNQIQLYTNW
                     MTVPLALLQGYAQLSILTNTSPPALRNFGFSGSGQILPTFATLGSVVAGTLFLVWIGE
                     LITERGVGNGISLIIFAGIVAGIPNLAGSSVFSASGVAAIMLLTVIALVLVIAIVIFN
                     EATRRVPVQYSRSVFRGGRMYRQSGQTYIPLKVNSAGMIPLLFAFSLMILPSFIAQYF
                     QSSSTGAIRDLAGWMRTAFNPTGVLYQVFVFFLVMVFTFFYTIIVFQQQNVAENLQKQ
                     GGFIPGIRPGRPTAEYINRVLFRITWAGALFLGAVSIVPFVATRLTGVQSLVISATGM
                     LIVVGVVIDTMKQLEAQLLMRNYEGFIR"
     gene            5143..5811
                     /locus_tag="VIM45_00520"
     CDS             5143..5811
                     /locus_tag="VIM45_00520"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020135804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEY8819801.1"
                     /translation="MYIILLGAPGAGKGTQAETLTRETGMPQVASGDLFRAVRTQDTE
                     LAVLVRSYYDKGLLVPDDVTIRMFLERLAQLDCANGAMLDGFPRTIEQAKALDDALAA
                     QGKRIDAVLYIKVSDPELMKRLAGRWICRNNGLHVFQSVSRPPKVPGVCDECGGELYQ
                     RVDDKEETARDRLRVFFKQTMPLVDYYLNQGKLAEVDGERAPDVVGRDLVQALRKLDT
                     AGAA"
     gene            5849..6613
                     /gene="map"
                     /locus_tag="VIM45_00525"
     CDS             5849..6613
                     /gene="map"
                     /locus_tag="VIM45_00525"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008480928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEY8819802.1"
                     /translation="MAVEVKSRDEIKVMREAGRIVAEVLEMLAAAVRPGQNVRRLDQI
                     VREEYARRGVTATFLGYPPKSKHPYPASVCVSINDQIVHGIPNDRTLEDGDIVSIDLG
                     VTHRGFVGDSAVTVPCGTVTPEARRLIETTKGAMWAGIHEARAGAHLGDVGYAIQSFA
                     ESRGYSVVRQYVGHGVGRQMHEEPQVPNYGEPGKGRRLQPGWVIAIEPMVNIGTHQTR
                     VGNDYWTVYTEDGSLSAHFEHTLAITKGEPEVLTLP"
     gene            6686..6904
                     /gene="infA"
                     /locus_tag="VIM45_00530"
     CDS             6686..6904
                     /gene="infA"
                     /locus_tag="VIM45_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012611021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEY8819803.1"
                     /translation="MAKKDAIEVEGVVKEPLPNAMFRVELANGHEVLAHISGKIRLHF
                     IKILPGDRVLVELSPYDLTRGRITYRFK"
     gene            6947..7060
                     /gene="rpmJ"
                     /locus_tag="VIM45_00535"
     CDS             6947..7060
                     /gene="rpmJ"
                     /locus_tag="VIM45_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005339020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEY8819804.1"
                     /translation="MKVRASVKPRCEKCKVIRRHGVLRVICEVPKHKQRQG"
     gene            7091..7471
                     /gene="rpsM"
                     /locus_tag="VIM45_00540"
     CDS             7091..7471
                     /gene="rpsM"
                     /locus_tag="VIM45_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006902862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEY8819805.1"
                     /translation="MARIAGVDIPRDKPVAISLRYLYGIGPTNANQIVDQARVNAATR
                     VRDLTDEEINRIREIIDKQFIVEGDLRREVRQNIQRLIDIQCYRGIRHRRNLPVHGQR
                     TRTNARTKRGARRTVAGKRRAGKK"
     gene            7524..7937
                     /gene="rpsK"
                     /locus_tag="VIM45_00545"
     CDS             7524..7937
                     /gene="rpsK"
                     /locus_tag="VIM45_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEY8819806.1"
                     /translation="MAERQARTGARQAQRGKKRERKSVPRGRAYVQSTFNNTIITLTD
                     PNGNALSWGSSGGSGFKGSRKSTPYAAQIAAEGAARRAMEHGLRQVDVYVKGPGSGRE
                     AAIRALQTSGLAVLSIRDVTPVPHNGCRPPKRRRV"
     gene            7973..8599
                     /gene="rpsD"
                     /locus_tag="VIM45_00550"
     CDS             7973..8599
                     /gene="rpsD"
                     /locus_tag="VIM45_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEY8819807.1"
                     /translation="MARYRGPDCRICRRHGEKLYLKGDRCMGPKCAIERRNFPPGPRS
                     TRRRKVSDRGLQLREKQKARYAYGVLEKQFRLYYEQAVKRPGVTGENLVRLLEQRLDN
                     TVHRSGWADSRDQARQVVRHGHISLNGRKTDIPSAQVKVGDVIGWTPSGRTTEYFKIR
                     QDMAPSTAAPGWLQLDREAMVARVIGVPARADADRTFDESVIVEYYSR"
     gene            8666..9772
                     /locus_tag="VIM45_00555"
     CDS             8666..9772
                     /locus_tag="VIM45_00555"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEY8819808.1"
                     /translation="MTQLETPQIEITESLDSYARVVADPLQPGFGTTLGNALRRVLLS
                     SLPGAAVTAVRIDEVEHEFSTIPGMKEDTTEFLLNMKELRLRGFSDRPGKLYLEVQGG
                     GPVFASQVQSTADYEIVNPELRLATLDSDEARLTVELTVERGRGYVPAGAVAGMPIGM
                     IPVDAIFTPVRRVNYNVERTRVGTASNYDRLVMEVWTDGTISGVEAVAQSSDILMAHF
                     SLFNHLGRPVAPVTERGLGSASMLPPDRYNTPIEDLNLSVRAYNCLKRSGLMTVGQVL
                     EKNEDELLSLRNFGRKSYDELRDKLIELGFLEPSSVTSRDGFGADYGGEDTGDDEEEL
                     GTLGAALMEALRGKSDGPSSGIDFLRRSDDDGEE"
     gene            9800..10147
                     /gene="rplQ"
                     /locus_tag="VIM45_00560"
                     /pseudo
     CDS             9800..10147
                     /gene="rplQ"
                     /locus_tag="VIM45_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011390413.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
     gene            10174..10956
                     /gene="truA"
                     /locus_tag="VIM45_00565"
     CDS             10174..10956
                     /gene="truA"
                     /locus_tag="VIM45_00565"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEY8819809.1"
                     /translation="MEQRSFALKLEYDGTAYGGSQFQANARSIQNEVERALAPMTLAA
                     ERTRVAFAGRTDAGVHATGQVAVVRVPANWTPDKLRRALNARLPEDIAVLAVVETAAG
                     FDPRRCARRRRYEYRILNGPVHSPLMRQSVWHVRHPLDDEKLRAAGALLVGKHDFAAF
                     GGPVDPPGASTVREMFATSVARNGFGLTLSFEANAFLPHQVRRMVGALVETGRGRLTV
                     EAFEMLLRQACPGSAGPAAPARGLCLVGITYEDVRFEMGEDG"
     gene            10953..11357
                     /gene="rplM"
                     /locus_tag="VIM45_00570"
                     /pseudo
     CDS             10953..11357
                     /gene="rplM"
                     /locus_tag="VIM45_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122452.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
     gene            11648..12040
                     /gene="rpsI"
                     /locus_tag="VIM45_00575"
     CDS             11648..12040
                     /gene="rpsI"
                     /locus_tag="VIM45_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012301481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEY8819810.1"
                     /translation="MQQHYFHGTGRRKTSVARCRLYPGTGTIIVNGKALEEVFTRPSH
                     QAVVQQPLRVTGQAGRFNVQIKVDGGGISGWAGAISHGISRALIVADENLKPTLRKQG
                     LLTRDARMKERKKYGLKRARKAPQYTKR"
     gene            complement(12088..12537)
                     /locus_tag="VIM45_00580"
     CDS             complement(12088..12537)
                     /locus_tag="VIM45_00580"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY8819811.1"
                     /translation="MATAELQTVIALPPPGVFEALCDLGRRPSIDPTITEISPPSGGL
                     QAGSTFSGRGTMASSGSGFDGIVTALERDSFLGLGYTLANGATMQEQWDLRPVPSGTL
                     VTYRAELRLPGGLLGKLLDRLMVSSGFQKQRETVLESIKTAFERRAT"
     gene            complement(12556..>13047)
                     /locus_tag="VIM45_00585"
     CDS             complement(12556..>13047)
                     /locus_tag="VIM45_00585"
                     /inference="COORDINATES: protein motif:HMM:NF019378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1614 domain-containing protein"
                     /protein_id="HEY8819812.1"
                     /translation="RRIPLPHVIFYVPPRITDQVVAINVGGAVIPLLVCLWLLPRAPF
                     LRTVLATAIVAVVSHLAATIVPGRGVEMPIWIAPASAALLGLLLTFGRGAAPLAYIAG
                     TLGALIGADITNLGALSQLGDGVLSIGGAGVFDGVFLAGIVATLLSFERPQRPRYTRP
                     IAT"
BASE COUNT         2726 a         3795 c         4310 g         2216 t
ORIGIN      
        1 tacgatcgaa tcgaccgcat ccgcggcatg gagatcacga tggtgacgtc ggcgcgaacc
       61 gacgaagaag gacggcggct gcttcagttg ctggggatgc cgttcagtaa gagttaagcg
      121 ttaggagacc gaccttggcc aaggtgagcc aaattgttag atctacccga aagccgaaat
      181 tttcgacgcg gcagaagaac cgttgcaagc tctgcggccg gccgcgtggc tacattcgca
      241 agtttgcgat gtgtcgcatc tgtttccgcg gactcgcgca cgtgggacag attccgggtg
      301 tgacgaagtc gagctggtag cgcggttgcg cctgctccac cgacagatgg ttgccggtac
      361 ggaatggcgc acgtgcagag cgagtccagg tgttggagac ccctgcacgg tttcggttga
      421 cggaggagaa ccgttccatg aatatgaccg atcccattgc cgatatgctg acgcgcattc
      481 gcaacgccgt ggcctcacgc cacgacacgg tgacgatgcc ggcgtccaaa gtgaaagcgg
      541 agatcgctcg cgtccttaaa gacgagcatt tcatccgcga cttcgaattg gtggaagagt
      601 cagaccgacg aggacacggc ccgcacaagg tcctgagggt acacctgagc tacagtggca
      661 agcgcgaagc catactgagc ggtcttcagc gcgtcagcaa gccggggttg cgggtgtacg
      721 tccagaagaa agagatcccg cgtgtatacg gtgggcttgg aatcgcgatt ttgtccacgc
      781 cgcagggaat catgaccggt caccaggcat ggcgccagca gatcggcggc gaggttctct
      841 gctacgtctg gtgactggtt tggcgcaagc cgcagccagc cgggtgatcg aggagcacaa
      901 aagcatgtca cgcatcggac gacaacccgt accagtgggg cagggcgtta atgtgatgat
      961 cgacggtaag gtcgtcaccg tgaaggggcc taaaggccag ttgcggcgtg agtttagttc
     1021 ggaggtcacc atcgagcagc gagacggcac tttggttgtc gctcgtcaag acgacgagcc
     1081 gcgccaccga gcgctgcacg gattgagccg cagcctgttg gcgaacatgg tggagggcgt
     1141 ctccaacggc ttcacgcggg tacttgaagt gcaaggggtc ggctaccgta tcgccgcaag
     1201 cgcgaacggc attactatgt cgctcggctt ttcccacggc gtcgaagttt ccgctccccc
     1261 cggaataacc tttgctgtgg aaggaacaaa ccgggtccgc atcgagggaa tcgacaaaga
     1321 actggtgggc caggtggcgg cgaacattcg cgccatccgc aaaccggagc cttacaaggg
     1381 caaaggcatt cgctaccagg gcgagaatgt gcgccgcaag gctggaaagg caggcaaggg
     1441 cgccaagggc gccaagtaat tacgttgata ggcgggcggt agggctcgca tgaggacttg
     1501 aagcatggta tccaaggtca aaacgagtcg agcccaacga ctccggcgac accgccgggt
     1561 tcgcaagcgc gtggcgggta cggaacagcg gccgcggctg gcggtattcc gcagcctggc
     1621 gcacatctac gcgcaggtga tcgacgatac tagtggctgc acacttgtgg cggcgtccga
     1681 tcgcgatgcg gacgtcgcgc cggaggcgaa aggaaagccg aagacgcaac gagccgaact
     1741 ggtcggcgtc gcgatcgctc gtaaggcaac ggcggcaggc ctgaagctgg ttgtcttcga
     1801 tcgcggcggt ttcctgtatc acggacggat aaaggctctg gcagaagcgg ctcgcaaatc
     1861 cgggttgagc ttctagagga tgatgcgacg atgacgactg gcgagcggat cgatccgcgc
     1921 gatctggaac tgaccgagaa ggtcgtgtac atcaaccggg tgtccaaggt ggtcaaaggc
     1981 ggccggcgct tcagttttag cgccctggtt gtcgtggggg atggccgagg ccacgtcggc
     2041 gccggcatgg gtaaagcgaa cgaagtgccc gaggcgatcc gaaaaggcgc aagcatcgcc
     2101 cgtaaaggac tgattcgcat tccgatgaac gccaatacaa tcacccaccc ggtgctggcc
     2161 cgattcgggg ccgcaaagat catgatgaag ccggcctcgg ccggaacggg agtgatcgcg
     2221 ggcggtggcg ttcgcgccgt catggaggtc gcgggagtca aagacatact ggcgaagtcg
     2281 cttggaagct cgaacgcgat taacgtcgtg cgcgccacca ttcagggcct tacggagctg
     2341 aaaaacgtgg ccgacgaacg ccgccggcga gtggaagccg cggaggctcc gatggaagac
     2401 gctatccggc gcggacgtcc gcccgtagtg cgtgtgccca gagaccggga ccgagatggt
     2461 ggaccgcggc gcggccctcg cgacgaccgg ccgcgtcctc agtttgaaca acgattcgag
     2521 ccgcgaatcg agcagggtca gccgggtgtc caagcgcaaa tggcaccggc cgttccagcg
     2581 ccggagcccg gggagcgaca gctgcctccg gcgccggccg agatgcagcc cgttgccgag
     2641 atcgctgcat cggagccggt aagggagtta gcgccaccgg agacggcggt cacagaggaa
     2701 gccggcaagg ttgaacagga gccggtcgcg gccgaggctg cgtctgctac cgctgaatcc
     2761 gggagcgccc cggcggagac agaggcggtc gcgcccgcca aacgcaagcg cgcggtggcg
     2821 gtatcaaaag cagtcgccct cgccgaagca gaaagcgcac cggctcaggc cgagaaggag
     2881 ccggccccga cccgaacccg cgcgactcgc gctaaagcgc cggcaaccgg tggaaacgag
     2941 cccgaaagcg aagcgtccgg aagttcggga gcagaacccg agggaacggt cacgcagccc
     3001 gaggcgactg ctgagccggc cccggctgcg gccgaagcga cgggggaaga gacggcgccc
     3061 aagccgcgcg ccaggcgaac gcgaaagatc gacgccgtga gctcggcgga agagtcatcc
     3121 gagaagccgc cggaaggctc gatcgaaagc gaaggctaat catggcaacg ctgcgcatca
     3181 cgtggaaaaa gagcgccatc ggctacgcga aggaccagaa actgacgatt cagtcgctgg
     3241 gattccgccg gctggggcag acggtcgagc aagaagacac gcgaagtacg cgtggtatga
     3301 tccacaaggt ccgacacctc gtgattgtgg aagagtagga gtaggcgcgc cgtggatcta
     3361 agccagatga aggcgactcc cggggcgcgt cacaagcgca tccgggtggg tcgcggcaac
     3421 ggcagtggca aaggcacgta ttcgggtaag gggctgaaag gtcagaaggc tcgaagcggc
     3481 agcggtctcc tgcccgggtt tgaaggcgga cagtttccgc tgattcggcg catggcccgc
     3541 aaacgcgggt tcaccaactt tgcacgcata gagtacgaat gggtgaacct tgaccagcta
     3601 gccgagctgc catccggcac tgtggtcaat caggaatccc tgctcgcggc cggcatcatc
     3661 aagaacataa agaggccgct caaggtgctc ggggacggag agatgacggg ggcgctgatg
     3721 gtgcaggcac ataagttctc cgcatcagcg cggcagaaga tcgaggcggc gggcggcacg
     3781 gtcgaggagg tcgcgggtgg cacagcctcc aacgtcatca ggggggaata ggccgcggct
     3841 gctacaggcc agcgtcgacg catttcggca accagacgtg cgagagaaac ttctcttcac
     3901 gttcgcgatg ctggtggtgt ttcgtctgct gacccacgtc ccgcttccga acgtcgacca
     3961 gaatgccttg accagcgcgt tcaaaggcaa cgcggtcctc ggcttcctca acgtcttcag
     4021 cggcaacgcg ctgcgaaagc tcagcgtcgc tgcactcggc gtctacccgt acatcacggc
     4081 atcgatcgtg atgcagattc tggtgccggt cattccgcgg ctgcaggcgc tttccaaaga
     4141 gggcgagcac ggccgtaatc agatccagct ctatacgaac tggatgaccg tacccctggc
     4201 gcttttgcag ggctacgcgc agctgtcgat cctgaccaat acgagcccgc cagcgctgcg
     4261 caacttcggt ttcagcggct ccggtcagat cctgccgacg ttcgcgacgt tgggatcggt
     4321 cgtcgcgggc acgctattcc tagtgtggat tggagaactg atcaccgagc gcggcgttgg
     4381 caacgggatc tcgctgatca tctttgccgg catcgtggcg gggatcccga acctggcggg
     4441 gagcagtgtc ttttcagcct caggcgtagc ggccatcatg ttgctcaccg tcattgcgct
     4501 ggttctggtg atcgcgatcg tgattttcaa cgaggcgacg cgacgtgtgc cggttcaata
     4561 ctctcgcagc gtgtttcgtg gcgggagaat gtaccggcaa tcgggtcaaa cgtacattcc
     4621 gctgaaagtg aactcggcgg gcatgattcc gcttctgttc gcgttctcgt tgatgatctt
     4681 gccgagtttc atcgcccagt atttccagtc ctcgtcgacc ggtgcgatcc gagacctcgc
     4741 cggttggatg cggacggcgt tcaatccgac gggggtgctc taccaggtgt tcgtcttctt
     4801 cctggtcatg gtgttcacgt tcttctacac gatcatcgtg ttccaacagc agaacgtcgc
     4861 cgagaacctg caaaagcaag gtggcttcat acctggcatc agaccgggaa ggccgacggc
     4921 ggagtacatc aaccgagtgc tgttccgcat tacctgggcc ggcgcgcttt tccttggcgc
     4981 cgtgtcgatc gtgccgttcg tcgctacccg cctgacaggt gtgcagtcgc tggtgattag
     5041 cgcgaccggc atgctgatcg tcgtcggagt cgtgatcgat acgatgaagc agctcgaagc
     5101 gcaactgctg atgcgcaact atgaaggatt tatacgctaa cgatgtacat cattcttctg
     5161 ggtgcaccgg gagccggaaa agggacgcag gcggaaacgc tgacccgcga aaccgggatg
     5221 ccgcaggtgg cgtcgggcga cctcttccgc gcggtgcgca cccaggacac cgagcttgcc
     5281 gtgctggtgc ggtcgtacta cgacaagggt ctgctggttc ctgacgacgt gacgattcgc
     5341 atgttcctcg agcgcctagc gcagctcgac tgtgcaaacg gagcgatgtt ggacggattc
     5401 ccgcgcacga tcgagcaggc aaaagcgctc gacgacgcgc tggcggcgca agggaagcgg
     5461 atcgatgcgg tgttgtacat caaggtatcg gacccggagc taatgaagcg gctcgcgggg
     5521 cggtggattt gtcgaaataa cgggctccac gtctttcagt ccgtcagccg gccgccaaaa
     5581 gtgccgggtg tgtgcgatga atgtggtgga gagctctacc agcgcgttga tgacaaggag
     5641 gagacggctc gcgaccggct ccgcgtcttc ttcaaacaga ccatgcccct ggtcgattac
     5701 tacctcaatc aaggcaagct ggcagaggtc gatggcgagc gggcgcccga cgtggtgggt
     5761 cgcgaccttg tccaggcgct acgaaaattg gacacggccg gagcggcgta attggcaact
     5821 atccgcagga actcgaggac agattatatt ggcggttgag gtaaagtcgc gtgacgagat
     5881 caaagtcatg cgcgaagcag ggcggatcgt cgccgaggtg ctggagatgc tcgcggccgc
     5941 ggtgcggccg gggcaaaacg tccgccgcct cgatcagatc gtgcgcgaag aatacgcacg
     6001 ccgcggcgta accgcgacct ttctaggtta cccacccaaa agcaagcacc catatccggc
     6061 gagtgtgtgc gtttcgatca acgaccagat cgtgcatggc atcccgaacg accgcactct
     6121 tgaagacggg gatattgttt caatcgacct cggagtgacc cacagaggtt tcgtcggcga
     6181 cagcgcagtg acagtgccct gcggaactgt tacgccggaa gcgcgacggc taattgaaac
     6241 gacgaagggc gcgatgtggg cagggattca cgaagcccgc gctggcgcgc acctgggcga
     6301 cgttggctac gcaatccaga gcttcgccga atcgcgcggc tacagcgtcg tgcgacagta
     6361 cgtcggtcac ggtgtcgggc gccagatgca cgaagagccg caggtgccga actatggcga
     6421 acccggcaag ggcaggcgtc tgcaaccggg atgggtcatc gccatcgagc cgatggtcaa
     6481 catcggaacg catcagaccc gggtggggaa cgactactgg accgtgtaca cggaagacgg
     6541 cagtctctcg gcacactttg agcatacgct tgcgattacg aagggtgagc cggaagtgct
     6601 tactctgcca tagagggtaa ttgtggacgc cgcgagcgtc cacgcgggac ctggatgggg
     6661 aagtggcccg tccatgaagg agtttatggc caagaaagac gcgatcgaag tcgaaggtgt
     6721 cgtaaaagag ccactgccga atgcgatgtt cagggttgaa ctggcgaacg gccacgaggt
     6781 tttggcccac atttcgggca aaatacggct tcactttatc aaaatccttc caggagacag
     6841 ggttttggtg gagttatcac cgtatgacct gacccggggc cgtatcacgt atcgcttcaa
     6901 atagacactt gtgctaactt gttgttttgg ggcaggagca gaccctatga aagtgcgtgc
     6961 gtcggtcaag ccgcgctgcg agaagtgcaa ggtcatccgc cggcacggtg tgcttcgcgt
     7021 tatttgcgag gttcccaagc acaagcagcg ccagggatga cggcttaaac agtccaggag
     7081 aaggttggga atggcacgta ttgccggagt tgacatccct cgagataagc ccgtcgcgat
     7141 ctcactccga tacctgtacg gtatcggccc gacgaatgcg aatcagatcg tcgaccaggc
     7201 gcgcgtcaac gcggcaacgc gggtacgcga cctgaccgac gaagagataa accgcattcg
     7261 cgagatcatc gacaagcagt tcatcgtcga gggcgacctg cgacgtgaag tgcggcaaaa
     7321 catccagcgg ctgatcgata tccagtgcta ccgtgggata cgtcaccgcc gcaacctgcc
     7381 ggtacacggt cagcgaacgc gaaccaacgc ccgcaccaaa cgaggtgccc ggcgcacggt
     7441 ggccggaaag cgacgggccg gcaagaagta aatcacggga gcgccaccgc ctaacgccgt
     7501 ggcgccaggc ctgaggagac cagatggcag aaagacaggc gcgcaccggc gcccggcagg
     7561 cgcagcgcgg caagaagcgc gagcggaagt cggtcccgcg cgggcgggca tacgtgcaat
     7621 cgacgtttaa caacacgatc atcacgctca ccgatccgaa cgggaacgcg ctttcctggg
     7681 gtagctcggg cggatcgggc ttcaagggct cgcgtaagag cacgccgtat gccgctcaga
     7741 tcgccgctga aggcgccgcc cgacgcgcca tggagcacgg cctgcggcag gtggacgtgt
     7801 acgtcaaggg acccggcagc gggcgtgagg cagcgatccg ggcgttgcaa acatcgggat
     7861 tggccgtact cagcattcgg gacgtcacgc cagttccgca caacggctgc cggcctccca
     7921 agcgtcgtcg cgtctaacgt atctattcaa ttagcgaaca aggaagttat caatggcccg
     7981 atacaggggg ccggactgcc ggatctgccg gcgtcacggc gagaagctat atctgaaagg
     8041 cgaccgctgc atggggccga agtgcgccat cgagcgtcgc aactttccac cggggccccg
     8101 ctcgacgcgg cgccgcaagg tctccgaccg cgggttgcag ctgcgcgaga agcaaaaggc
     8161 gcgctatgcc tatggcgtac tcgagaagca attccggctg tattacgaac aggcggtgaa
     8221 gcgaccgggt gtaaccggcg agaatttggt gcggctgctg gaacagcggc tcgacaacac
     8281 ggtccaccgg tccggttggg ccgattcgcg cgaccaggcg cgacaggtcg tgcgccatgg
     8341 ccatatttcg ctaaacggcc gtaagaccga tatcccatcg gcccaggtca aagtcggcga
     8401 cgtgatcggt tggacgccga gcggacgcac gactgaatac ttcaagatca ggcaggatat
     8461 ggctccgtcg actgccgcac cgggctggct gcagttggat cgtgaggcaa tggtcgcccg
     8521 cgtgatcggc gtaccggcga gggccgacgc ggatcgcact tttgacgaga gcgtcatcgt
     8581 cgagtactac tcgcgctagg ctcaatttga gtagacctgt gctatcgctc gcagattccg
     8641 ggccggcgtc cggggaggaa catcgttgac ccagttagag accccgcaaa tagagatcac
     8701 cgaaagtctc gacagctacg cacgcgtggt agccgatccg cttcaacctg gcttcggcac
     8761 cacacttgga aatgcgctca ggcgcgtgct gctcagctcg ctgccggggg cggcagttac
     8821 cgccgtgcgg atagatgaag tcgagcacga gttctcgacc atcccgggca tgaaagagga
     8881 taccaccgag tttttgctga acatgaagga actgcgcctg cgcggcttct ccgatcggcc
     8941 tggcaagctg tatctcgagg tccagggtgg aggccccgtg tttgcctcgc aggtgcaatc
     9001 taccgcagac tacgagatcg tcaacccgga gctgcggctg gcaacgctgg attccgacga
     9061 ggcgcgcttg acggttgagc tgactgtgga gcgcgggcgc gggtatgtgc ccgctggtgc
     9121 ggtcgccggc atgccgatcg gcatgatccc cgtcgacgcc atatttaccc cggtgcgacg
     9181 cgtcaactac aacgtcgagc ggacgcgcgt gggtacggcg tcgaactacg atcggctggt
     9241 gatggaggtg tggaccgacg gcaccattag cggcgtcgag gcggttgcac agagctctga
     9301 catcttgatg gcgcacttct cgctgttcaa ccatctgggc cgcccggtgg ccccggtcac
     9361 ggagcgcggc cttggctctg cttcgatgct gccgccggac cgctataaca cgccgattga
     9421 ggacctcaac ctttcggtcc gggcatacaa ctgcttgaag cgcagcggtt tgatgaccgt
     9481 aggccaggtg ctcgaaaaga acgaggatga gctgctttcg cttcgtaact tcggccgtaa
     9541 atcgtacgac gagttgcgag acaagctgat cgagcttggc ttcctcgagc cgtcgtcagt
     9601 cacaagtcgt gacggtttcg gcgcagacta cggtggcgaa gacaccggtg acgacgaaga
     9661 ggaactcggc acgctgggtg cagcgctcat ggaggccctg cgcggtaaga gcgacggtcc
     9721 gagcagcggc atcgatttcc ttcgccgctc agacgacgac ggggaggagt agcgtcaccg
     9781 ggcgctgcga atagtagcta tgcggcatca agtaagcgga cgcaagttca atcgaaacac
     9841 cggccatcga acggctatgt atcgcaacct cgtgtccgat ctgctgaaac acgagaagat
     9901 caagaccacg gagcccaagg cgaaagagat ccgctccatg gcggagaaga tcatcacgct
     9961 gggtaaagac ggcaccatac acgcacggcg acaggcgctg aaatttgtgt acgatcccgc
    10021 ggtggttgcc cacgtgttcg ccgagcttgg accgcggtac gcgaccaggc ccggcggcta
    10081 tacccgtatc attaaagttg ggcctcgtct cggcgatggt gcgccgatgg cgattatcga
    10141 gctggtctga acaccgccgg ccggcgtccc accgtggaac aacgaagctt cgcgttgaag
    10201 ctggagtacg acggaactgc ctacggcggg tctcagttcc aggccaacgc gaggagcata
    10261 caaaacgagg tagagcgggc gctggcgccc atgacgctgg ctgcagaacg gacccgagta
    10321 gcgttcgcgg gtcgaaccga cgccggagtc cacgccacgg ggcaggtcgc cgtcgtgcgg
    10381 gttccggcta attggacgcc ggacaaactg cggcgggcat tgaacgcaag gctgcccgag
    10441 gacatcgcgg tcctggcggt ggtggaaacg gccgccgggt tcgacccgag gcgctgcgcc
    10501 aggcgccgta gatacgagta ccggatactg aacgggccgg tgcactcgcc gctcatgcgc
    10561 cagagtgtat ggcacgtaag gcatccgctg gacgacgaaa agctgcgggc ggctggagcc
    10621 ctgctggtcg gaaaacatga ctttgcggcc ttcggagggc cggttgaccc gccgggggcg
    10681 agcacggtcc gggaaatgtt cgccacctcg gtggcgcgaa acggcttcgg gctgacgctg
    10741 tcgttcgagg cgaacgcctt cctgccgcac caggtgcggc ggatggttgg cgcgctggtc
    10801 gaaactggaa gaggtcgcct gaccgtcgag gcgttcgaaa tgctgctgcg gcaggcttgc
    10861 cccggatctg ctggtccggc ggcgccggca cgcggtctgt gcctggtcgg gatcacgtac
    10921 gaggacgtac gattcgagat gggtgaagac ggatgaaaac gtacagtaca agggcgtccg
    10981 aggtcatgcg ctcctggcat gtcctcgatg cgacgggcgt gccgctgggc aggctcgcca
    11041 cgcacgtcgc gacattgatc cgcggaaagc acaaggtcaa ctacgccccg catctcgaca
    11101 ccggagacta cgtcgtcgtc gtcaacgcgg aaagagttgg actaaccggc cgcaagtttg
    11161 agcagaaaaa atactatcgc cattcgcagt acccgggcgg tcttaaagtc cgaaccgtgc
    11221 gcgagcagat ggggcgaaac gcgaccaggg tcatcgagca cgccgtattt ggaatgttgc
    11281 cgcacaacag tctcggacga tcaattcacg gccatttaaa agtgtacgcg ggaccggatc
    11341 acccgcacgc ggcgcaggta acgggaccag cacaaccggc cctttctcaa ccgcccaagg
    11401 aagggagccg caggaaacac aggcctgtcg tggcccgcgc cgtcgccccg gatgcggcac
    11461 cagttcagga taccgttggc cccgttacga ttgcgggagc ggcggaggcc ggcatcgcag
    11521 cggggtcgca gccggctgcc gtgccggccg agcaggaaca gaccgcaacg aacatggagc
    11581 aggccgtgcc cacgaccgca ctgccttccg aggctgatga gacaactgag gagcctaaag
    11641 cgtgacgatg caacagcatt acttccacgg caccggtagg cgtaagacgt cggtcgctcg
    11701 ctgccggctc tatccgggca ccgggacgat catcgtcaac ggcaaggccc tggaagaggt
    11761 attcacccgt ccatcgcacc aggccgtcgt gcagcagccg cttcgcgtca ccgggcaggc
    11821 cggacgcttt aatgtgcaga ttaaagtcga cggcggtgga atcagcggct gggccggcgc
    11881 catttcgcac ggtatctcgc gggcgctgat cgttgccgac gaaaacttaa agcccaccct
    11941 ccgcaagcag gggctactca cgcgcgacgc ccgcatgaaa gagcggaaga aatatggact
    12001 caagcgtgct cgaaaggcgc cccagtacac caagcgctga ccgatccgaa attcgttacc
    12061 ggggcggctg ctaggccatt cctctgttta cgttgcacgg cgctcgaacg cggtttttat
    12121 cgactccagt actgtttctc gctgcttttg aaagccgctc gataccatta gtcggtccaa
    12181 aagcttgccg agcaagcctc ccggaagccg taactccgct cggtaggtga ccaacgtacc
    12241 gcttggcacc ggcctcaggt cccactgctc ttgcattgtc gcgccattgg caagcgtgta
    12301 tccaaggccg aggaacgagt cccgctcgag tgcggtaacg atgccgtcga atccgctgcc
    12361 tgagctggcc atcgttcccc tgccgctgaa cgtcgaccct gcctgcagcc cacccgaagg
    12421 tggagagatt tcggtgatgg tcgggtcgat cgaaggtctg cgtccgaggt cgcaaagcgc
    12481 ctcaaatacg cccggcggag gcagggcgat cacggtctgt aactcggcgg tcgccaagaa
    12541 ttgacggtcc tcgtattacg ttgcgatcgg gcgggtatag cgcggccgtt gtggtcgttc
    12601 gaaactgagt agtgtggcga cgatgccggc caaaaagacg ccatcgaaca ccccggcccc
    12661 gcctatactt aagacgccgt ctcccagttg actcagcgca cccaggtttg tgatgtcggc
    12721 gccgatcagc gctcccagcg ttccggcgat gtaggcgagc ggcgccgcgc cgcgcccaaa
    12781 cgtcagcaag agtcccagca gcgccgccga tgctggagca atccaaatgg gcatctcaac
    12841 gccccgaccg ggcacgatcg tcgcggccag gtgggagacg acggcgacga tcgctgttgc
    12901 gagcaccgtc cgcaagaagg gtgctcgtgg cagcaaccat aggcacacga gcagcggaat
    12961 caccgcgccg cccacgttga ttgcaaccac ctggtcggta attcggggtg gaacgtagaa
    13021 aatcacgtgc ggaagcggga tgcggcg
//
LOCUS       DATCPE010000016         3901 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U7219
            k141_293757, whole genome shotgun sequence.
ACCESSION   DATCPE010000016 DATCPE010000000
VERSION     DATCPE010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955033
            Sequence Read Archive: SRR1586279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 16:49:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 640
            CDSs (total)                      :: 631
            Genes (coding)                    :: 627
            CDSs (with protein)               :: 627
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3901
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_293757"
                     /isolate="SMAG_U7219"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="VIM45_00590"
     CDS             <1..286
                     /locus_tag="VIM45_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HEY8819813.1"
                     /translation="RAVESRTPHIPMLSDLRESGSIEQDADVVMFIYREDAYMRKDDW
                     QDTHPDQPGRSYPTGIASIIIAKHRNGPTGQVDLRFHNRTTKFEDLQLTD"
     gene            286..1056
                     /locus_tag="VIM45_00595"
     CDS             286..1056
                     /locus_tag="VIM45_00595"
                     /inference="COORDINATES: protein motif:HMM:TIGR01446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HEY8819814.1"
                     /translation="MGKQSMNAGGSPSSINERTPENRGPARLGGFPAGARQTAIPSVF
                     FSDVLPAIDDPAELLVTLYAFYLVSRRRGSRRWFDEAELAAEPPLMRSLAKVNPDSAA
                     ALRGGLDAAVNRGSLLRAVRHRDGRSLRLYAVHMPGAAEMLASLDARVHRPVDGAGGA
                     DRQYPLPNIFVLYEENIGVISPLLADQLGTAEADYPWPWIEAAFREAVALNRRSWRYI
                     ERILERWRTEGPDLEAIRRSAEGGRKSLAGRYWRLVKR"
     gene            1133..2392
                     /locus_tag="VIM45_00600"
     CDS             1133..2392
                     /locus_tag="VIM45_00600"
                     /inference="COORDINATES: protein motif:HMM:NF013828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8819815.1"
                     /translation="MRRAVEFGHPDFGRAIPCVCVLTEHEDERQERLQRFSNLGALAR
                     QSFATLLPRGRSPEASHQERFARALADAVRYAEDPQGWLVFVGESGTGKTHLAAAIAN
                     QCIENGVPVFFAVVPDLLDHLRAAYGPASEIPYDRLFEVVRNAPVLVMDALGTQFSTP
                     WAEEKLFQVLNHRYNSQLPTVFTCSIALDDLDERIRTRISDPALSRIHVLEDGPGVSR
                     SLPDPLTLALVRGMTFPTFAFKPAPPQITEQVSKNLQRVLLAARNFADHPEGWLVLLG
                     ETGCGKTHLAAAIAHHIAGQRQQVRFVVVPDLLDYLRKANYEVKDGRDAIDEMRTVPI
                     LVLDDLGVHSATPWAQEKLFQILNYRYNAKLATVITLSCSLDDLPQSWVSRMYDDKVS
                     MIQEIQAPDYRGLQRGKKSEPTRRTRR"
     gene            complement(2399..3853)
                     /locus_tag="VIM45_00605"
     CDS             complement(2399..3853)
                     /locus_tag="VIM45_00605"
                     /inference="COORDINATES: protein motif:HMM:NF039823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5667 domain-containing protein"
                     /protein_id="HEY8819816.1"
                     /translation="MLGIRRHNRERELADALSASLEAIRGGGSSVDDCLQRYSSLAAE
                     LEPLLNAGSRMQALSRITPNEQARLNARFALMRSAAASRRPVSIMPRAAVRRRSWLAL
                     APAGIAVVLFAAVAVPVLGSIDTNAVPGDWNYSFKRATERVRLAIATDPTDRRLLRIE
                     FARRRLTEIERLSSNGQVDVHAGEITALIKNYSSDLSQVATAAQTQGLIQSSEKQQLE
                     AVTSQAQAVLQPIAENATQDKPVKAVAQEAVSVTKQANSVVKAIPTQQAKQSNQASPS
                     PSNTPTPAPPVPADVSSAASATASATPSPASPTAAPSPTPSATPSATATSTNTVGAAP
                     TREAAVLTPVPTAPPARPAQTQPATEPATTTAGVPVAARTSTSEPPAASIPVRSVVTA
                     TATPVPTRAAPIEAGLIVRDRGPESIVYQWTGAATSLDQIVAPLGNQVVFVTYLRADG
                     QSVVWYPGTAAPVIGPSALLTVKLRVITTPTNGR"
BASE COUNT          710 a         1212 c         1254 g          725 t
ORIGIN      
        1 gcgagcggtg gaatctcgta cgccgcacat ccctatgctg agcgacctgc gtgaatctgg
       61 aagcatcgaa caggacgccg acgtcgtcat gtttatctat cgcgaagatg cctacatgcg
      121 gaaggacgac tggcaggaca cccatccgga ccaaccggga cgtagctacc caacgggcat
      181 cgcgtcaatc atcatcgcca agcacaggaa tggaccgacg gggcaggtag atctacgatt
      241 ccacaaccgc acaacgaaat tcgaagacct gcaactcacc gactgatggg taaacagagc
      301 atgaatgccg gcggatcgcc aagcagtatt aacgaacgaa ctcccgagaa ccgcggaccc
      361 gcgcgactgg gcggattccc tgccggcgct cgccagacgg cgataccgag cgtgttcttc
      421 agcgacgtgt tgccggcgat cgacgacccc gccgagctgc tcgtcaccct gtacgctttc
      481 tatctggtga gccgccgccg gggcagccgc cgctggttcg acgaggcaga gctggctgcc
      541 gagccgccac tcatgcgttc gctggcaaag gtaaacccgg attcggcggc tgcgctccgc
      601 ggcggtctgg acgccgccgt gaaccgtggg tcgttgttac gggccgtcag acaccgcgac
      661 ggcagatccc tccggttata cgcagttcac atgccgggtg ctgcggagat gctggcttcg
      721 ctcgacgcga gggtccatcg tccggtcgat ggcgccggcg gcgcggacag gcaatatccg
      781 ctgcccaaca tttttgtgct gtacgaagag aatatcggcg tgatctcacc attgctggcg
      841 gatcagcttg gcaccgcaga ggcggattat ccctggccct ggatcgaggc ggcatttcgc
      901 gaggccgtgg cgctgaaccg tcgcagttgg cggtatattg agcgtattct cgaacgctgg
      961 cgaaccgagg gaccggacct tgaagcgatt agaagatctg ctgagggcgg gcggaagagc
     1021 ctggcgggac gatactggcg cctcgtcaag cgctgatctt ccggccacgt ttctggtcgg
     1081 tgaacgcggt tcgggggatc cgaactgtcc ccagtgctca ggcgcggggt tcgtgcgccg
     1141 tgcggtagag tttggccacc ctgactttgg tcgcgcaatt ccatgcgtct gcgtcctcac
     1201 cgaacatgag gacgaacggc aggagcgctt gcagcggttc agcaaccttg gcgcgttggc
     1261 tcgacagagc ttcgcgacgt tgctgcctcg cggccgcagt cccgaagcat cgcatcagga
     1321 gcggttcgct cgcgcactcg ccgacgccgt acggtatgca gaagacccgc aggggtggct
     1381 cgtgttcgtg ggggagagcg gaaccggcaa aacccacctg gcggccgcga tcgccaatca
     1441 atgcatcgag aacggagttc cggtcttttt tgcggtcgtg ccagacctgc tcgatcacct
     1501 gcgcgcggcg tatggaccgg cgagcgagat accgtatgac cgtctatttg aagtagtgcg
     1561 caatgcgccg gtgctggtga tggacgcact cgggacacag ttcagtactc cgtgggcgga
     1621 agaaaaactg ttccaggtgc tcaaccaccg ctacaacagc cagttgccga cggtgttcac
     1681 gtgctcgatc gcgctcgacg accttgacga gcgcattcgc acacggatct ccgatccggc
     1741 tctgtcgcgg attcacgttc tcgaggacgg gcccggagtg tcgcgcagct tgccggaccc
     1801 gttgacgctg gcgttggttc gcgggatgac cttcccaacg ttcgccttta agccggcgcc
     1861 gccgcagatt acggagcagg tctcgaagaa cctccagcgc gttttgctgg ccgcccgcaa
     1921 cttcgcggac caccccgagg gctggctcgt gctgctcggc gagaccggct gtggtaagac
     1981 gcaccttgcc gcggcaatag cccatcacat cgccggccag cgtcagcagg tccggtttgt
     2041 cgtggtgccc gatcttctgg attacctgcg caaggcgaac tacgaggtca aagacggccg
     2101 cgacgcgatc gacgagatgc gaacagtgcc aatcctcgta ctcgacgatc ttggcgtaca
     2161 cagcgccacg ccatgggctc aggagaaact tttccagatc ctcaactatc ggtacaacgc
     2221 caagctggcc acggtgatca cgctgtcatg ctcgctcgac gatctgccac agtcgtgggt
     2281 atcccgcatg tatgacgaca aagtgagcat gatccaagag atccaggcgc ccgattaccg
     2341 cggtctgcag cgcggaaaga agtccgaacc cacaagacgc acgcggcggt agaggacctt
     2401 accggccatt ggtcggcgtg gtgatgactc gaagcttgac ggtcagcagg gcacttgggc
     2461 cgatcacagg ggctgccgtc cccggatacc agaccaccga ctgaccatcg gcccgcaggt
     2521 atgttacgaa gacgacctga ttgccgagcg gcgccacgat ctgatcgagc gatgtggccg
     2581 cgccggtcca ctgataaacg atcgattccg gtccccggtc gcgcacgatg agtccggcct
     2641 cgattggtgc agcgcgtgtg ggaactggcg tagccgtcgc ggtgacgacc gaccgtacgg
     2701 gtatactcgc tgcaggcggc tcagaggttg acgtccgcgc tgctaccggg acccccgcgg
     2761 tggtcgtcgc cggctcggtc gcgggttggg tctgcgctgg tcgcgcgggc ggtgctgttg
     2821 gcacaggcgt gagcaccgcg gcctcacgtg ttggcgcggc ccccactgta ttcgtcgaag
     2881 tggcggttgc ggaaggtgtt gcagatggcg tcggagaggg tgccgcggtt ggagaggcag
     2941 gcgacggtgt ggccgatgcc gtcgcggacg ctgcactact tacatccgct ggcactggcg
     3001 gagcgggcgt gggcgtgttc gaaggcgatg gcgacgcctg atttgactgc tttgcctgct
     3061 gcgtcgggat cgccttgacg acgctattcg cctgcttggt gacggaaacc gcctcctggg
     3121 caactgcctt gaccggctta tcctgcgtcg cgttctcggc gatcggctgc aacacggctt
     3181 gtgcctgcga cgtcaccgcc tcgagctgct gcttttcgga gctttgaatc aggccctgcg
     3241 tttgcgcggc ggtggcaacc tgactcaggt cggacgagta gtttttaatt agagccgtga
     3301 tttcgcccgc gtggacatcc acttgtccat tgctggaaag gcgctcaatc tccgtcaatc
     3361 gccgccgggc gaattcgatg cgcagtagcc ggcgatcggt aggatccgtc gctattgcca
     3421 gccgtacgcg ctccgtcgcc cgcttgaagc tgtaattcca gtcgccagga accgcgttgg
     3481 tatcgatgct gccgagcacg ggaacggcga ccgctgcaaa cagcaccacg gctatcccgg
     3541 caggggcgag cgccaaccac gagcgtcgcc gcaccgcagc ccggggcatg atcgataccg
     3601 gccgccgcga cgccgcggcc gaccgcataa gagcaaatct cgcgttcagt cgcgcctgct
     3661 cgttcggggt gatgcggctc aacgcttgca tccgcgagcc cgcgttcagc agcggttcga
     3721 gttcagcggc aaggctcgag tagcgctgca ggcaatcgtc gaccgaagac ccgccgccgc
     3781 gaatcgcttc aagcgacgcg gatagcgcat cagcgagctc tcgctctcga ttatgcctgc
     3841 gaattcccag catcctgctg actcattacc ttccgaagct tgcctaacgc cttgaacgtc
     3901 a
//
LOCUS       DATCPE010000017         3069 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U7219
            k141_391487, whole genome shotgun sequence.
ACCESSION   DATCPE010000017 DATCPE010000000
VERSION     DATCPE010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955033
            Sequence Read Archive: SRR1586279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 16:49:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 640
            CDSs (total)                      :: 631
            Genes (coding)                    :: 627
            CDSs (with protein)               :: 627
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3069
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_391487"
                     /isolate="SMAG_U7219"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..443
                     /locus_tag="VIM45_00610"
     CDS             <1..443
                     /locus_tag="VIM45_00610"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY8819817.1"
                     /translation="WRNVIDTNLTGSFLMSRAVARQLIKQNSGGRIVNISSIAGKTHG
                     ANTAAYAASKAGLQSLTASMAAELAQYQVTVNAVLPGMIDTFRMDDLGRGENWERMIK
                     QRVPLGRAGTGEDIAHMTVFLCSPQADWITGQCYVVDGGTVRRG"
     gene            496..870
                     /locus_tag="VIM45_00615"
     CDS             496..870
                     /locus_tag="VIM45_00615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8819818.1"
                     /translation="MLLTPMASRLLSDMEDSHKALVEHSDADQPVRHGFRAICEAVSA
                     ALADLTEDQLVAAPSVDEWSMVEVVEHVTEHDRKYHEVEQHGVAHYVEHGLEHALQLW
                     KLRHDQLPQQAEGPHDRRSGTL"
     gene            958..2040
                     /gene="ruvB"
                     /locus_tag="VIM45_00620"
     CDS             958..2040
                     /gene="ruvB"
                     /locus_tag="VIM45_00620"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEY8819819.1"
                     /translation="MNDESDERLVSGQEQLEDERLDTTLRPKYLADYIGQEKVKDNLH
                     ISIKAALARGEGLDHLLLYGPPGLGKTTLANIIGAEMGVNVRTTSGPAIERPGDIASM
                     LTNLQTGDLFFVDEVHRLPRIVEEVLYAAMEDFVLDLVIGKGPGARSMRIPLQHFTLV
                     GATTRYDMISPPLRDRFGSVYRLEFYTDEALTLIVKRSARILNVSVDPDAAVEIARRS
                     RGTPRIANRLLRRVRDHAQVLHDGVITLGVARESLARLEVDDLGLDDVDRIILRTIVE
                     KFEGGPVGLSSLAASTAEDRDTIETVYEPFLLRLGFIMRTPRGRVVTRAAYRHLGVTP
                     PPEHQDPQRAFLWPSDDQTVQQDGSA"
     gene            2276..2488
                     /locus_tag="VIM45_00625"
     CDS             2276..2488
                     /locus_tag="VIM45_00625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8819820.1"
                     /translation="MYIFKSCPKCRGDLALEQGSVNGKLLGPDTDFACLQCGYYLQPA
                     ERRDLIVRIVKSHRSRRRAAQLPAAA"
     gene            complement(2494..>3069)
                     /locus_tag="VIM45_00630"
     CDS             complement(2494..>3069)
                     /locus_tag="VIM45_00630"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HEY8819821.1"
                     /translation="PVIAFIRGTGASGAYYIACATQRIIAQRSSIVGSIGVITIRPQM
                     QDLLDKIGVQVSVTATGPLKGAGLPFREETEAERVNMRLMVDAFFERFIDVVADGRRV
                     SRERARQWATGEVFWANRALDEGLVDELGDLERAVAVASSMVNIPEKNAVTVRPRVPL
                     AQRLLKGVGSAAASSLHAEFERLFVPRIEYR"
BASE COUNT          595 a         1029 c          933 g          512 t
ORIGIN      
        1 tctggcggaa cgtgatcgac accaacctca ccggcagctt tcttatgtcg agagccgtcg
       61 cccgccaact gatcaaacag aacagcggcg ggcggattgt caacatctcc tcgatcgccg
      121 gtaagacgca cggcgcgaat accgcggcat acgcggccag caaagccggg cttcagtcgc
      181 tcacggcctc aatggcggcg gagcttgccc agtaccaggt gaccgtaaac gccgtgctgc
      241 ccggaatgat cgatacgttc cgcatggacg atctgggacg gggcgaaaac tgggagcgga
      301 tgatcaagca gcgggtaccg ctcggtcggg ccggcaccgg cgaagacatc gcccatatga
      361 ccgtcttcct ttgttccccg caggcggact ggatcaccgg ccagtgctat gtcgtcgacg
      421 gcggcacggt acgccgaggg tgagttgcag gccggttcag catcgattcg gtttcgaggg
      481 aggagtctgg catcaatgct gctgacacca atggcatctc gcctgctatc agatatggag
      541 gattctcaca aggcgctggt cgagcacagc gacgccgatc agcccgttcg gcacggcttt
      601 cgcgccatat gcgaggcggt gtccgcggcg ctggccgacc tcacggagga tcaactggtg
      661 gccgcgccct ccgtcgacga atggtcgatg gtcgaagtcg tggaacacgt caccgagcac
      721 gaccgcaagt accacgaggt cgaacagcac ggcgtcgccc attatgtcga gcatggcctc
      781 gaacacgcgt tgcagctctg gaagttgcgg cacgaccagc tgccgcagca ggctgaagga
      841 ccgcatgaca ggcgttctgg tacactgtag acagtatcag aacatgtgta ctgatacgcg
      901 aacacaaacg cgatccggga gcgggtgcag gtggcaacaa cggcagcgcc gggcacgatg
      961 aacgacgaat ctgacgagcg gctggtctcc ggccaggaac agctcgagga cgagcggctc
     1021 gacaccacgc tgcggccgaa gtacctcgcc gattatatcg gccaggaaaa agtcaaagac
     1081 aacctgcaca tcagcatcaa ggcggcgctc gctcgcggag aagggctcga tcacctgctg
     1141 ctctacggtc ctcctggact cggcaagaca acgctggcga acatcatcgg cgccgagatg
     1201 ggcgtcaacg tccgcaccac ctccgggccg gcgatcgagc ggcccggcga catcgcttcc
     1261 atgctcacaa atcttcaaac aggcgatctg ttcttcgtgg acgaggtcca ccggttgccg
     1321 cgcatcgtcg aggaagtcct ctacgcggca atggaggact tcgtcctcga cctggtgatc
     1381 ggcaagggac ccggagcccg cagtatgcgc atccccttgc agcacttcac gctcgtcggc
     1441 gccacgactc gctacgacat gatcagcccg ccgctgcgcg accgcttcgg ctcggtctac
     1501 cgcctcgagt tctacaccga cgaggcgctc accctgatcg tcaagcgcag cgcccgcatc
     1561 ttgaacgtct ccgtcgaccc cgacgccgcc gttgagatcg cccgccgatc acgcggcacg
     1621 ccgcgcatcg ccaaccgcct cttgcgccga gtccgtgacc acgcgcaggt gctccacgac
     1681 ggcgtgatta cgctcggcgt ggcccgcgaa tcgctcgctc gtctggaagt ggacgacctc
     1741 gggttggacg acgtcgaccg catcatcctg cgcacgattg tcgagaagtt cgagggcgga
     1801 ccggtcggtc tctcttcgct cgcggcatcg acggcagagg accgggatac catcgagacg
     1861 gtgtatgagc cgtttctgct ccgcctgggg ttcatcatgc gcacccctcg cggccgcgtc
     1921 gtgacccgcg ccgcctaccg ccacctcggc gtaacaccac cccccgagca ccaggaccct
     1981 cagcgagcct tcctctggcc gtccgacgac cagaccgtcc agcaagacgg ttcagcttag
     2041 ggacccgcac tgcacatatg gcgcgtgatc agcccatgca tgactcgaag tacgtctgcc
     2101 ccgacagaca tggtggtgcc atctatcgcg cgccgagcct tcggtaccgg acgatactgg
     2161 ccaaaagtac tgatcgcttc gcgactaagg ccgcatgcgc gtcgctatcg cgccgcctag
     2221 actcggtgat gtcgaatcgg gcagattcag cccggttcgc cacggaggaa ccgacatgta
     2281 catcttcaag agttgcccga agtgccgcgg cgacctcgcc ctggaacagg gcagcgtgaa
     2341 cggcaagctg ttgggacccg acacggactt cgcttgcctg caatgcggct actacttgca
     2401 gcccgctgag cgacgcgacc tgatcgtgcg gatcgtgaaa tcgcatcgta gccggcgccg
     2461 cgccgcccaa ttgcccgccg cagcgtaacg gcgctaccgg tattctatcc gtgggacaaa
     2521 caggcgttcg aactcagcgt gtaatgacga ggcggccgcc gaccccacgc ccttgagcaa
     2581 gcgctgcgcc aggggaacgc gcggccgcac cgtgacggca ttcttctccg gtatgttgac
     2641 catggacgac gcgaccgcta cggcgcgctc caggtcgccc aactcgtcga caagtccttc
     2701 gtcgagcgcc cggttggccc agaacacctc gccggtagcc cattgtcgcg cccgctcgcg
     2761 cgagacacgc cgtccatcgg caacaacatc gatgaaccgt tcgaagaacg cgtccaccat
     2821 caaccgcata ttcacgcgct cagcctcggt ttcctcacga aatggcagtc cggcgccctt
     2881 cagcgggccg gtggccgtca cgctcacctg caccccgatc ttgtcgagca gatcttgcat
     2941 ctgcgggcgg atcgtgatca cgccgatgct gccgacgatg ctggaccgct gcgctatgat
     3001 gcgctgggtc gcgcaggcga tgtaataagc gccggacgcg ccggtgccgc gaatgaaggc
     3061 gatgaccgg
//
LOCUS       DATCPE010000018         8698 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U7219
            k141_132384, whole genome shotgun sequence.
ACCESSION   DATCPE010000018 DATCPE010000000
VERSION     DATCPE010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955033
            Sequence Read Archive: SRR1586279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 8698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 16:49:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 640
            CDSs (total)                      :: 631
            Genes (coding)                    :: 627
            CDSs (with protein)               :: 627
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8698
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132384"
                     /isolate="SMAG_U7219"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..911)
                     /locus_tag="VIM45_00635"
     CDS             complement(<1..911)
                     /locus_tag="VIM45_00635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEY8819822.1"
                     /translation="MPEMRQVYVVGVGMTRFGRYSHMDGGGLGAEAVRLALKDGGLLL
                     KDVQVLTSGSYFESSGAFSSGAVGFHEAVLRVATGEVDVAMAIGSDVTSGDRRGRRAA
                     APPGGPVLSPAPDFIQQGYAGAVREYSEHHGFSQSQAAKVAVKNRRHGSLNPFACLQT
                     EIRHDDVLRSPMVEYPNTEAMCCFAIDGAAALIVASAEKARAIVGAGALIRVAASCRI
                     TNPRQNQPDALRDIAGMTRICATQAYQQAAIEPGELDCVELDDYLAGAELLHYETLML
                     CEEGGAGRMIDESRTSLGGDIPVNTSGG"
     gene            1104..1592
                     /locus_tag="VIM45_00640"
     CDS             1104..1592
                     /locus_tag="VIM45_00640"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY8819823.1"
                     /translation="MKTAEHTRDTSELLEAAVFHLLTAGAEYWARVDLTMPQLKVLLL
                     LGRHGSAPVSWLASRMKVSPPNITGILDRLQQRHWVQRTSDPQDRRVVRILLTPEGEK
                     LLHELSGAGVDYLRESISSMPNRGTDSLRQGLSAFLESLGQHASPADNNYELRGHVAA
                     RS"
     gene            complement(1589..2203)
                     /locus_tag="VIM45_00645"
     CDS             complement(1589..2203)
                     /locus_tag="VIM45_00645"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEY8819824.1"
                     /translation="MTGPRRIIGVVLAAGLSSRMGMPKALLDWNGEPLIAYQVRQLFA
                     AGVGTVVAVTGHEAKRVEEALAGTGAITIRNHHYREGRATSVRAAAEAITNEPEVIVL
                     LNVDQPRRSTVTERLLREHSRLGGCITVPAYAGRRGHPVILSGTLLPELRLVSEEDQG
                     LKAIMRRHERERREVAFEEEEVILDLNDPDAYARAVNGWRQPAG"
     gene            complement(2200..2583)
                     /locus_tag="VIM45_00650"
     CDS             complement(2200..2583)
                     /locus_tag="VIM45_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HEY8819825.1"
                     /translation="MAEDPYSPTVLDHFTNPRNAGVMTDPDAVGVQTNPVCGDTMKLM
                     LRIEHDTVTEARWQTVGCPAAIATSSIATELIVGKRLTEIEGLTKETIVAAAGGLPAS
                     KLHCSVLAQDALRRAIETYRAKCSA"
     gene            complement(2576..3052)
                     /locus_tag="VIM45_00655"
     CDS             complement(2576..3052)
                     /locus_tag="VIM45_00655"
                     /inference="COORDINATES: protein motif:HMM:NF017771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEY8819826.1"
                     /translation="MAIQTGQQAPKLTLTTTDGSVMVPDSAGRPTLLMFFQEAGTPAC
                     SLQVASLNGESETLKDLDALAVCVSTDPIERLSEFASSLGGVTLALATDPEGHAAEAF
                     GVYDAEQKRAQRSAFVIAPNGTILLAIAWYNPSNSDQLLSIFTALGVGEAGKGPNG"
     gene            complement(3052..4263)
                     /locus_tag="VIM45_00660"
     CDS             complement(3052..4263)
                     /locus_tag="VIM45_00660"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEY8819827.1"
                     /translation="MYFDRNHQPGSGSALVGEGLALDRFGSTTDNAVLRDMIRREIGD
                     RGPMTFRRFMQLALYEPSEGYYRTRAAIGPRGDYLTSPELHPLFGAILFRQLVEFWEL
                     LDRPDPFAVAEVGAGTGSLARSIISAANGWEFGGCLKYEIIESGTLQREAQRATLGAL
                     AEQVVWTDVLPESGDCSCVLSNELLDSFPVHRVTARDEKLLEIFVGLNGDRFVDLFAE
                     PSTPGLQAYFERAGVFPADGAVAEVNLDLDGWHLSVSRAVRRGFVLTLDYGYPASTLY
                     APWRKQGTLHCYSRHTVSTDPYQRIGRQDMTAHVDFTSVVRSGRDSGFELVAFTSQQN
                     FLPALGIGEALAGGPVVAASLEEFLARRRAVEALLDPEGLGRVRVLVQGRVVGRPALR
                     GFPGSTGDGLL"
     gene            4360..4791
                     /locus_tag="VIM45_00665"
     CDS             4360..4791
                     /locus_tag="VIM45_00665"
                     /inference="COORDINATES: protein motif:HMM:NF012354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY8819828.1"
                     /translation="MEPHVKLWLEEDGRLVLSDYRARLLQLVHDSGSLAQAAAAMGLS
                     YRRAWGKVRELERNLGVPLVQSDVGGTGGGRSRLTGEAAALLRQYDAFTYDLTVEMAL
                     LRDRHFGDRFGRPAPHAGLRESGEQIEPVANDPVDPPGGEL"
     gene            complement(4720..5403)
                     /locus_tag="VIM45_00670"
     CDS             complement(4720..5403)
                     /locus_tag="VIM45_00670"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEY8819829.1"
                     /translation="MTICAPFVAPNGSPAVDRIAHRAGNSRRALQLAIEAGVDWIEID
                     VWYSYGRLIARHEWGVWRVPLVYDSRRLHVLRHRLIDLEDVIRLTEGGPRLFIDLKGV
                     HRRLPGAIVRALRKHDAADRAAVCGQHWPPLDAIAEAEPRIEIFHSLGRPEHLEAYAA
                     REHDRLVPAGISIARSLITPELVENFHKQERKLFAWTVNDWEQASQLAAWRVDGIISD
                     RLDLLAALA"
     gene            complement(5366..6199)
                     /locus_tag="VIM45_00675"
     CDS             complement(5366..6199)
                     /locus_tag="VIM45_00675"
                     /inference="COORDINATES: protein motif:HMM:NF024762.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HEY8819830.1"
                     /translation="MIMQLTPAAESFGRIIGAGGGIDLLRASVAVGLVAEPALDVEQI
                     VRAIENLAIDVRFEIGDVSGADTVIAGLHHALFVKRGFRGNRSHYYDPRNCYVHEVLT
                     SRTGMPITLSVLYIVIGRHLGMQVDGIGFPAHFVVRAGADASHYRYFDPFNEGQELSR
                     ADLAIYLKQQGGDPEKQLDLFLAAVTARQILSRLLTNLKNCYVNLSDYARAREIVNLM
                     LVLAPWAIEERRDRGLLSSYIGDVATAHADLSLYLDLAPNAPDAPRVRDHLRAFRGAK
                     R"
     gene            6332..6793
                     /locus_tag="VIM45_00680"
     CDS             6332..6793
                     /locus_tag="VIM45_00680"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020644602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEY8819831.1"
                     /translation="MAEIEVGKEAPDFTVNDENRQPVHLADLRGRPVVLVFYPLDFSP
                     VCTGEMCEIRDRFPDVEAAGARLFGISRDSTWAHKAFKEKEGLKQTLLSDMKGEVSTA
                     YGAWNERAGLAERLTVVIDKNGKIAFMDRSPGIPTPREQQKMIAAVQQLAA"
     gene            6913..7182
                     /locus_tag="VIM45_00685"
     CDS             6913..7182
                     /locus_tag="VIM45_00685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8819832.1"
                     /translation="MTDQWLVRTLVRFRECTFRHLEAELLAIRGVPPSDVVASVLKLE
                     QAGLVERKPVSGIGAEERLFRLTRRGKRMGALVPDAPRSPTIFYE"
     gene            complement(7191..>8698)
                     /locus_tag="VIM45_00690"
     CDS             complement(7191..>8698)
                     /locus_tag="VIM45_00690"
                     /inference="COORDINATES: protein motif:HMM:NF017964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HEY8819833.1"
                     /translation="TKPADQGNQRAVAAEYRRWRSGCTEVLTSNEIFNAVLDQALNDV
                     RTLWNADGQSSGFLAAGSPWFDTLFGRDSLIASLQLLAFTPEVARNTLIRLGSEQGTV
                     VDEWRDEEPGKILHELRSGEMTRTGELPFGRYYGSIDSTPLYLVLAAEYWRWTGDLRL
                     MRRLKPNLVACLEWAKRWGDRDGDGYLEYQREAAEGLLNQGWKDSGNAIVHRDGQLAM
                     PTIALVEVQAYLYAGLMGLAEIADALHDTKLAGNARHQARSLRRRFNRDFWIPDSRFY
                     ALALDGKKKRVESIASNPGHALFAGIVPLERGPAVAARLMQGDMFTGWGIRTLSAGAA
                     TYNPMGYHIGSVWPHDNSLIAAGFKRYGQEPLLNDLASALYDTARSMEYYRLPELFCG
                     LPRSVEGQPVPYPVACRPQAWAAGTIPMLLTSILGLCPNAPGGALSIVCPQLPGWLDR
                     VDVRNLHVAGRSLNLRFELRNRRTHLTVVDSGGLNVGVVKRWPRQQRRASR"
BASE COUNT         1709 a         2650 c         2705 g         1634 t
ORIGIN      
        1 ccgccgctcg tgttcaccgg gatgtcgccg ccgagcgagg tccggctctc gtctatcatg
       61 cgtccggcgc cgccttcttc gcataacatc agcgtctcgt agtgaagcag ctccgcgccg
      121 gcaaggtagt cgtcgagttc aacacagtcc aattcgccgg gttcaatggc cgcctgctgg
      181 taggcctgag tagcgcagat acgggtcatg ccggcgatgt cgcggagcgc atctggctgg
      241 ttttgccggg ggttcgtaat gcggcaagat gcagcgaccc ggatcagtgc gcctgctccc
      301 acgatcgcgc gcgctttttc ggcagacgcg acgatgagcg cggcggcacc atcgattgca
      361 aagcagcaca tcgcctcggt attgggatat tcgaccatag gactgcgtag cacgtcgtcg
      421 tgtctaatct cagtctgcag gcaggcaaac gggttaagcg aaccatgacg tctgtttttg
      481 acggccacct tcgcagcctg tgactgactg aacccgtggt gctcagagta ttcgcggaca
      541 gccccggcat agccttgctg tatgaagtcg ggagccggcg atagtacggg gccaccaggc
      601 ggcgcggccg cacgacgccc acgacgatcg ccggaagtca cgtcggatcc gatcgccatc
      661 gcgacgtcca cttcccctgt tgccactcgc aagacagctt cgtgaaatcc gacagcgccg
      721 gaggagaacg cgccgctcga ttcgaaataa ctgccggagg tcagtacttg aacgtctttg
      781 agcaacagac caccgtcttt gagcgccaat cgaacggctt cggcgcccaa cccgccgcca
      841 tccatgtggc tgtagcgacc gaaccgcgtc atgccaacgc cgaccacgta gacctggcgc
      901 atttcgggca tctgattcgt ctccgggaat tccatcataa agaccgcaac gctaatactt
      961 tctgtacata gcagtaaatt gactcagtag gcgtatactt gatgaagcac tttgctgggc
     1021 gatgacaaca tccgccgccg taatatctta acgagacaat acgcgcacat cggaacaagc
     1081 gcgataagga taaggtatcg actatgaaaa cggcggagca tacgagggat acgtctgaat
     1141 tactcgaggc agccgtcttt catctgctta cggccggcgc ggaatactgg gcgcgtgtcg
     1201 atctaacgat gccccaactg aaggtattgt tgttgcttgg acggcatgga agtgccccgg
     1261 tgagctggct ggcgtcacgc atgaaggtat cacctccgaa catcaccgga atccttgaca
     1321 ggctgcagca gcgccactgg gtacagcgga cgagcgaccc ccaagatcgg cgcgtcgtgc
     1381 gaattctcct gacgccggaa ggcgagaagc ttctgcacga actctccgga gcgggggtgg
     1441 actacttgcg ggaaagcatc agctcgatgc cgaacagggg tacggactcg ctgcgtcagg
     1501 gactatctgc atttctcgag tcgctcgggc agcacgcctc tcccgcggac aataattacg
     1561 aattacgcgg ccacgtagcg gcgcgttctt aacccgcggg ttgccgccag ccgttcacag
     1621 cccgagcgta tgcatccggg tcgttcaggt cgaggattac ctcttcttcc tcaaacgcga
     1681 cctctcggcg ttctcgctcg tggcgtcgca tgattgcctt cagtccctga tcctcctccg
     1741 acacgaggcg caactccggc agcagcgttc cgctgagaat tactggatgg cctctgcggc
     1801 ccgcgtacgc aggtacggtg atacagcctc caaggcggga gtgctctctt agtaatcgtt
     1861 ccgtaaccgt tgagcggcgg ggctggtcga cattgagcag cacgattact tcaggctcat
     1921 tcgttatcgc ttcggcggcg gcccgtaccg acgttgctct accttctcga tagtggtgat
     1981 tgcgaattgt gatcgcacca gtgccggcaa gcgcctcctc gacacgtttg gcctcgtgac
     2041 cagtgacggc tacaacggtg ccaacaccgg ccgcaaagag ctgccgaacc tggtaggcga
     2101 tcagcggctc gccgttccaa tccaggagcg ctttcggcat gcccatccgg ctcgaaagcc
     2161 cggcagccaa aacaacgccg atgatgcgcc gaggaccggt catgccgagc acttcgcccg
     2221 ataggtctcg atcgcccgcc ggagggcgtc ctgcgcgagc actgaacagt gcaatttact
     2281 ggcggggagg ccgcctgcgg cagcaacgat cgtttccttc gtcaacccct cgatctcggt
     2341 tagccgtttg cccacgatca gttcggtggc aatcgagctt gtcgcgatcg ccgcgggaca
     2401 gccgaccgtt tgccagcgcg cctccgtgac ggtgtcgtgc tcgatgcgca gcatcagctt
     2461 catcgtgtcg ccgcagacag ggtttgtctg tactccgacg gcgtccggat cggtcatcac
     2521 gccggcgttc ctcgggttgg tgaagtgatc gagcacggtc ggactgtacg gatcttcagc
     2581 cattcggacc ttttcccgct tcaccgacgc ccaacgcggt aaaaatcgac aataattgat
     2641 cgctgttcga agggttgtac caggcgatcg ccagcaaaat ggtgccgttc ggggcgatga
     2701 cgaacgccga ccgttgggcc cgtttctgtt ctgcgtcgta gacgccaaag gcttctgccg
     2761 cgtgcccttc cggatcggtc gcgagcgcga gggtcacgcc tccaagcgag ctggcaaact
     2821 cggagagcct ttcgatagga tcggtgctga cacagacggc cagagcatcg aggtccttca
     2881 gggtctctga ttcgccgttg agcgacgcaa cctgcaggga gcatgcaggg gtgcctgctt
     2941 cctggaagaa catgagaagg gttgggcggc cggcggagtc cggtaccatc actgacccat
     3001 cggttgtggt cagcgtcaat ttgggcgcct gctggccggt ctggatcgcc atcacagcag
     3061 gccgtcaccg gttgagccgg gaaacccgcg cagggccggc cggcctacga cgcgcccctg
     3121 gacaagcacc cgaacgcggc ccagcccttc tgggtcgagc aacgcctcaa cggcacggcg
     3181 ccgggcaagg aactcttcca ggcttgctgc cactaccgga ccaccggcga gcgcctcgcc
     3241 gatcccgagg gcagggagga agttctgctg gctggtgaat gctacgagtt caaaaccgga
     3301 atcgcgcccg ctccggacaa cggaggtgaa atcgacgtgg gcggtcatgt cttgcctgcc
     3361 aatccgttga tacgggtcgg ttgagactgt gtgtcgtgag tagcaatgga gtgtgccctg
     3421 ctttcgccag ggtgcgtaca gcgtcgaggc ggggtaaccg taatccaacg tcagtacgaa
     3481 gccgcgcctc acggcacggg aaactgacaa atgccaaccg tcgaggtcca agtttacctc
     3541 agcgacagcg ccatccgcag gaaaaacgcc ggcccgttcg aagtatgcct gcaagccggg
     3601 cgtcgatggc tcggcgaata gatctacgaa ccgatcaccg ttgagaccga cgaagatttc
     3661 aaggagcttc tcgtcccgag ccgtcacccg gtggacggga aagctgtcga gtagctcgtt
     3721 actcaagaca caggaacagt cgccagattc cggcagcaca tcggtccaga cgacctgctc
     3781 ggcgagcgcg cccagagtag ctcgttgcgc ctccctttgt agcgtgcctg actcgatgat
     3841 ttcgtacttc agacacccgc caaattccca gccgttcgcg gccgaaatga tcgagcgcgc
     3901 cagcgatcct gtaccggcgc cgacctcggc aaccgcgaac ggatccggcc ggtcgagcaa
     3961 ttcccagaac tcgacaagct gacggaagag gatcgcgccg aataacgggt gcagctccgg
     4021 gctggtgagg tagtcgccac gcggtccgat cgccgcgcgc gtgcgatagt acccttcgga
     4081 gggctcatag agcgccaact gcatgaaccg gcgaaaggtc atgggaccgc ggtcccctat
     4141 ctctcgccgg atcatgtcgc gtaggaccgc gttgtcggtg gttgaaccaa accgatcgag
     4201 ggcgagtccc tcaccaacca acgccgatcc agagcccggt tgatggttgc gatcgaaata
     4261 catacgccat catgctagcg ccggtgccgc acgcgggtcg aggcggtacg atgacgtgga
     4321 accacggggt acgcaatcgc cgttcacgag ggtggaccgg tggaacctca cgtcaagctc
     4381 tggttggaag aagacggcag gctggtgctc agcgactatc gcgcccgcct gctgcaactc
     4441 gtccacgaca gcggctcgct ggcgcaggca gccgcggcga tggggctctc gtatcgacgg
     4501 gcgtggggca aagtgcgcga gctcgagcgc aaccttggcg tgccgctcgt acagagcgat
     4561 gtcggaggga ccgggggtgg tcgctcccgg ctcactggcg aggccgccgc gctgttgcga
     4621 cagtacgatg cgtttacata cgacttgacc gtcgagatgg cgttgttgcg tgatcgacac
     4681 ttcggcgatc ggttcggccg gcccgcgcca cacgcggggt tacgcgagag cggcgagcag
     4741 atcgagccgg tcgctaatga tcccgtcgac cctccaggcg gcgagctgtg acgcttgttc
     4801 ccaatcgttg accgtccaag cgaagagctt gcgctcttgc ttgtgaaaat tctcgaccaa
     4861 ttcgggtgtg atcagcgacc gggcgatcga gatgccggcc gggacgaggc gatcgtgctc
     4921 tcgcgcagcg tacgcctcga ggtgctcggg acggcccagg gaatggaaga tctcgattcg
     4981 aggctccgcc tccgcaattg cgtccaacgg cggccaatgc tgtccgcaga cggccgcgcg
     5041 gtccgcggca tcatgtttgc gcaaagccct gacaatcgcg cccggcaggc gccgatgcac
     5101 tccctttaag tcgatgaaga ggcggggacc gccctcggtc aatctgatga cgtcttcaag
     5161 gtcgattaag cggtgccgca ggacgtggag acgcctgctg tcgtatacga gaggaacacg
     5221 ccatacaccc cactcgtgcc tagcgatcag tcggccgtag ctgtaccaga cgtcgatttc
     5281 gatccaatcg acgcctgctt caattgcaag ctggagcgct cgccggctgt tgccggcgcg
     5341 gtgagcgatg cgatccacgg ccgggctacc gtttggcgcc acgaaaggcg cgcaaatggt
     5401 cacgtactcg gggcgcatca ggagcgtttg gagcgagatc gaggtacagc gacaggtcgg
     5461 cgtgtgcggt agcgacgtcg ccaatgtacg agctcagcag cccgcggtca cgacgttctt
     5521 caatcgccca gggcgcgagc acgagcatca ggttcacgat ctctcgcgct cgggcatagt
     5581 cgctaaggtt gacgtagcag tttttcagat ttgttagcag cctcgaaagt atctgccgtg
     5641 cagtcaccgc ggcaaggaag agatcaagct gtttttccgg atcgccgcct tgctgtttga
     5701 ggtagatggc caggtcggcg cgggataatt cctgtccttc gttgaacgga tcgaagtacc
     5761 gatagtgcga agcgtccgcg ccggcgcgaa cgacgaaatg agcgggaaag ccgatgccgt
     5821 cgacctgcat tcccagatgg cgtccgatca cgatgtaaag caccgacagc gtgatcggca
     5881 tgccggtgcg gctggtcagg acttcgtgga cgtagcaatt gcggggatcg taataatggc
     5941 tgcggtttcc gcggaaccca cgcttgacga agagggcgtg gtgaagaccc gcaatcacgg
     6001 tgtccgcacc ggaaacgtcg ccgatttcaa agcgcacgtc gatcgcgagg ttctcgatgg
     6061 cacgtacgat ctgttcgacg tcaagcgcgg gctcggcgac gagtcccacc gcaaccgacg
     6121 cgcgcagcag gtcgatgccg ccgcctgctc cgattatcct tccaaacgat tctgcagcgg
     6181 gcgtaagttg catgatcatc ccgtttgaaa tcctacttca atggaacgac aggagccacg
     6241 gcgctatgat cccaattacc acgccgccca actatcggtg gcgcttttct ctggcccggc
     6301 ggtgggttaa ctggttggaa ggagacgcga tttggcagag atcgaggttg gtaaggaagc
     6361 accggatttc acggtcaacg acgaaaatcg ccagccggtg cacttggccg atttgcgcgg
     6421 tcggcctgta gtgctcgtgt tctacccgct cgatttttcc ccggtgtgca ccggcgaaat
     6481 gtgcgaaatc cgcgatcgct ttcccgacgt tgaggccgcg ggagcccggc tgtttggcat
     6541 cagccgggac agtacttggg cccataaggc attcaaagaa aaggaagggc tgaagcaaac
     6601 gttgctgtcc gatatgaaag gcgaagtttc gacggcctac ggcgcatgga acgaacgcgc
     6661 ggggcttgcc gagcgattga ccgttgtgat cgacaagaat gggaagatcg cgtttatgga
     6721 ccgcagcccc ggtattccca cgccccgcga acagcagaag atgatcgccg ccgttcagca
     6781 gctagcagcg taacgcaacg gcgcgcgacc ttgcgtcgat ggctctcgac ccattgccaa
     6841 cccaccatgg atctcttcgg atcgattcga cgcataactg gcttggggcc gaggcgcgag
     6901 catgacctgg acgtgaccga ccagtggctt gtgcggacgc tggtgcgttt tcgtgaatgc
     6961 acctttcggc acctcgaggc tgaactgctc gcgatccgtg gtgtgccgcc atccgacgtc
     7021 gttgcctcgg tgctgaagct cgaacaagcc ggtctggtag aacgcaagcc ggtctcgggc
     7081 atcggcgccg aggaacgtct cttccgcctg acaaggcggg gtaaacggat gggggccctc
     7141 gtaccggatg cgccgcgcag cccgacgatc ttctacgagt gaacgctgat ttagcgagag
     7201 gctcgccttt gttgtcgcgg ccagcgcttc acgacgccga cgttcaggcc gccactgtca
     7261 accacggtta ggtgagtacg gcggttgcga agctcgaacc gcagattcaa tgatcgtccg
     7321 gcaacgtgga ggttccgaac atctacccgg tcaagccatc cgggaagctg cgggcaaacg
     7381 atcgaaaggg cgccgccggg cgcatttgga cagagaccga ggatcgaggt gagcagcatc
     7441 ggaatggtgc ctgccgccca tgcctggggg cggcaggcca ccggatacgg aaccggttgc
     7501 ccctcgaccg aacgtggcaa gccgcaaaac agctccggca accggtagta ctccatcgat
     7561 cgcgccgtgt cgtaaagggc gctggcgagg tcgttcagta gcggttcctg accgtagcgc
     7621 ttgaatcccg cggcgatcag cgaattgtcg tgaggccaaa cgctgccgat gtggtagccc
     7681 atcgggttgt acgttgcggc gcccgcgctc aacgtacgga taccccaacc agtgaacata
     7741 tcgccctgca tcagccgcgc cgcgaccgcg ggaccccgtt cgagcgggac gatgcccgcg
     7801 aagagcgcgt ggcccggatt tgacgcgatc gattcgacgc gtttcttctt gccatcgagt
     7861 gcaagagcgt aaaacctcga atcaggaatc cagaaatccc ggttaaaccg acgtcgcagc
     7921 gaacgcgcct ggtgccgtgc attgccggcc agcttagtgt cgtgaagggc gtcggcgatc
     7981 tcggcaagcc ccatcaaccc cgcgtacaga taagcctgga cctcgaccaa tgcgatcgtc
     8041 ggcatcgcca gctggccgtc ccggtgcacg atggcgttgc ctgagtcttt ccacccctgg
     8101 ttgagcagcc cctcagccgc ttcgcgttgg tactcaagat agccgtcgcc atctcggtcg
     8161 ccccaacgtt ttgcccactc gagacacgcg accaagttcg gcttcaggcg gcgcatgagg
     8221 cggaggtcgc cggtccaacg ccaatactca gcggctagca cgagatacag tggcgtcgag
     8281 tcgatgctgc cgtagtaacg accgaaagga agctcacctg tgcgcgtcat ttcacccgat
     8341 cgaagctcgt gcaagatctt acccggttcc tcgtcgcgcc attcgtcgac gaccgtaccc
     8401 tgctccgaac cgaggcgaat caacgtgttg cgagcgacct ccggggtgaa tgcgagtagc
     8461 tggagtgaag cgatgaggct gtctcgccca aagagcgtgt cgaaccaggg gctgccggcg
     8521 gctaggaacc cggacgattg gccatcggca ttccataacg tgcgcacgtc gttcagcgcc
     8581 tggtccagca ccgcgttgaa aatctcattg ctggtgagaa cctctgtaca ccctgaacgc
     8641 cagcggcggt attcggcggc gaccgcgcgc tggttcccct ggtccgcggg ctttgtgc
//
LOCUS       DATCPE010000019         2357 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U7219
            k141_294419, whole genome shotgun sequence.
ACCESSION   DATCPE010000019 DATCPE010000000
VERSION     DATCPE010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955033
            Sequence Read Archive: SRR1586279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 16:49:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 640
            CDSs (total)                      :: 631
            Genes (coding)                    :: 627
            CDSs (with protein)               :: 627
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2357
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_294419"
                     /isolate="SMAG_U7219"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            758..1183
                     /locus_tag="VIM45_00695"
     CDS             758..1183
                     /locus_tag="VIM45_00695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8819834.1"
                     /translation="MNDRGIVVAGIPVPLSSPVFLSIVAIHVAAGLVCVVAGVAAMLS
                     AKRAGRHPFAGSVYYWSLLVVFLSMTALSVIRWPRDNHLLVLGVLSFGAAVVGRAARR
                     RWRPPLIVGSPGKPPARVKVKASLGLRGNKSVFLLNRAP"
     gene            complement(1296..>2357)
                     /locus_tag="VIM45_00700"
     CDS             complement(1296..>2357)
                     /locus_tag="VIM45_00700"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HEY8819835.1"
                     /translation="VVLIDRERQAVFQPSLLWVAVGDRTPEAIQRPLATLVRKGVEIR
                     IGTVEKLDAETRTVRVAGEDLVGDAVIVALGADLAPETIPGLADGGHNLYTLGGARSF
                     RDELAALTTGRVAVLTASPAYKCPAAPYEAAMLIAGALRRRGIRDRMQIDLYSAEAGP
                     MLVTGPQLSAAVRGIVESKGIAYHPEHQVTKVDVAARRLIFANGATADFDLLLYVPPH
                     RAPAVVRDAGLLSESGWVAADRRTFETRVAGVYAIGDVTGIPLAMGKPLPKAGVFAHA
                     EAEVVAHNIASAWMGRGARRTFSGEGQCFVETGGGRAGLGAGNFYAEPTPQVKLYAPA
                     RRWHWGKIILERRWLKRWI"
BASE COUNT          412 a          740 c          760 g          445 t
ORIGIN      
        1 ttcgcagaat cggctcgcgc aagtagagac tcgagggaat tggatgcctg cgcgcacgct
       61 ggcgacacga gtaaagtggt tgctacgaat gagagcagcg cgggtagcgc ggatcgatac
      121 aatcccgcac gccaggtcag attgaagcga agcatagtcc gattttgaaa gagtccacga
      181 gacagcgcgg caaacgaagg aagcgcgcga atccctccga cacatccggt ttggatggat
      241 cggaactaca acttcttatg ttgtgtggtg gactacgccc gaggaggtgg gagctcgggt
      301 cgtgacgtta cggagagaag cgttattgcg gcgccaggca ttacccgctc gggaacgtgg
      361 ccggttctgt ggagaacaga cgtggccgaa gcgagcgcgg ccgccgcaca cggcaccatc
      421 gactgacacc catcaggtgt ccacgggaaa ttgcagggag ccgccggcgc cttctgaacg
      481 ggtgcgacgt cttgtgcggt cgcctgccca ttggcgccgc ccgatgtcat caccatcgcc
      541 gtcatatcct ctgagctgcc cggcgtcgtg catgcgaagc cgctcgtgac cagaacgaag
      601 gcgacgagga ataggcttgt gaggagaccg attagacgcg aggggcgctg catcatgtta
      661 acaatatgct gacgtgaaga gccacgaaag ggtcactgct tcgcatcgtt gactggaatt
      721 gcgtgatttt atgggaatat tctaggcacc ggttcatatg aacgatcgcg gtattgtcgt
      781 cgcaggcatt ccggtcccgt tgtcatcgcc ggtgttcctg agcatcgtcg ccatccatgt
      841 cgcggcaggg ctcgtctgcg tggttgccgg ggtcgcggcg atgctgtcag cgaagcgcgc
      901 tggcaggcat cctttcgccg gtagcgtcta ttattggagc ctactggtcg tgtttctttc
      961 gatgaccgcg ctctcggtca ttcgatggcc gcgggataat cacctgctcg ttctcggcgt
     1021 tctgtctttc ggggcagcag tcgtcggccg cgcggcgaga cgccggtggc gaccgccgct
     1081 tatcgtcggt tcacctggca agccgccggc ccgtgtgaaa gtgaaagcct cgctcgggct
     1141 ccgcggcaac aagagtgtat tcctcttgaa tcgcgcgcct taggccgtcg acatcgagtc
     1201 cttcaatggc ccgacttaca ccatgcaagg acgtgtcgat tttggcggtc atggagatcc
     1261 tcccgaatta cgtgttgata cggcccgaac cacgctcaga tccatcgttt cagccatcgg
     1321 cgttccagga taatcttccc ccagtgccat cgtctcgcgg gtgcgtacaa tttgacctgc
     1381 ggtgtcggct cggcgtagaa gtttccggcg ccgagtcccg cacggccgcc accggtctcg
     1441 acgaagcatt ggccttcacc gctgaacgta cggcgcgccc cgcgtcccat ccacgcgctg
     1501 gcgatgttgt gagcgaccac ctcggcttcc gcgtgcgcaa agacgcccgc tttcgggagt
     1561 ggcttcccca ttgcgagcgg gatcccggtg acgtcgccga tggcgtagac gccggcgaca
     1621 cgagtttcga acgtacggcg gtcggcggcc acccagccgc tctcgctgag gagtccggca
     1681 tcgcgtacga ccgccggcgc ccggtgcggc ggcacgtaca agagcaggtc gaagtcagcc
     1741 gtggccccat tggcgaatat caggcggcgc gccgccacat cgaccttcgt cacctggtgc
     1801 tccgggtggt acgcgatgcc cttcgactcg acgatcccgc gcacggcggc ggacagttgc
     1861 gggccggtca ccagcattgg gccggcctcc gccgaataga ggtcgatctg catgcggtcc
     1921 cgaattccgc ggcgacggag tgcgcctgcg atcagcatcg ccgcttcgta cggcgctgca
     1981 gggcacttgt acgcgggcga cgcggtgagc acggcgaccc ggccagtcgt cagcgccgcc
     2041 aactcgtcgc ggaaggacct cgccccgccc agcgtataca gattgtgacc gccgtcggcg
     2101 aggccgggaa tcgtctccgg tgccaaatcg gcgccgagcg ccacgatcac cgcgtcgcct
     2161 acaaggtcct cccctgcgac gcgcaccgtg cgcgtctcgg cgtcaagctt ctcgacggtg
     2221 cctatgcgga tctcgacgcc ttttcggacg agcgtcgcga gcggccgctg aatcgcttct
     2281 ggcgttcggt cgccgaccgc gacccagagc agcgacggct ggaagaccgc ctggcgttcg
     2341 cggtcgatga gcaccac
//
LOCUS       DATCPE010000020         6097 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U7219
            k141_489281, whole genome shotgun sequence.
ACCESSION   DATCPE010000020 DATCPE010000000
VERSION     DATCPE010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955033
            Sequence Read Archive: SRR1586279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 6097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 16:49:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 640
            CDSs (total)                      :: 631
            Genes (coding)                    :: 627
            CDSs (with protein)               :: 627
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6097
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_489281"
                     /isolate="SMAG_U7219"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..681
                     /gene="coaE"
                     /locus_tag="VIM45_00705"
     CDS             <1..681
                     /gene="coaE"
                     /locus_tag="VIM45_00705"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEY8819836.1"
                     /translation="RVDPHSGRLLRLNRRRHNTGDWCVRMIVIGLTGGIASGKSTISG
                     ALAEHGATVVDADKVGHDVYRPATEGWQKVVDAFGKQILAPDGEIDRKALGAIVFGDP
                     AHRERLQAIVWPLMKGMMRRLIDDFRNQGIGVAVIEAAVLIEADWVDLVDQVWVVTVP
                     VEVAAARLIGRNGLTPDQARARISAQLSNEERARQADVVIDNSGSVEQVRSRLAELWD
                     GLVLAGSR"
     gene            796..1713
                     /locus_tag="VIM45_00710"
     CDS             796..1713
                     /locus_tag="VIM45_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015412965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbbQ/NirQ/NorQ/GpvN family protein"
                     /protein_id="HEY8819837.1"
                     /translation="MTTDTEQQDHFRQYAIEEYTPKREPFYVGINDEEQLFRAAFEQK
                     IPVLLKGPTGCGKTRFVEYMAYVLGRPLTVIKEKAGSGGEPQNNGAGDGHGLSVVTVA
                     CHEDLTAGDLVGRYLLEGEGTRWMDGPLTRAVKRGAICYLDEVVEARKDTTVLIHPLT
                     DHRRLLPVEKLGQVIEASEGFLLVISYNPGYQSALKDLKHSTRQRFIALDFTYPPRET
                     EAKIIEHEAGIDAATAMELAKLGEKVRNLREHGLGEGVSTRLLIYAGKLIAKGITPRR
                     ACQVGVVWALSDDAEVQRSIEEVVSSIFE"
     gene            1720..2340
                     /locus_tag="VIM45_00715"
     CDS             1720..2340
                     /locus_tag="VIM45_00715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YfcE family phosphodiesterase"
                     /protein_id="HEY8819838.1"
                     /translation="MKIGIVSDIHCNIAGLDRALAEMGDVDELICAGDSIFQYRFSNE
                     VVTRLRERGARVILGNHEETFFSSAGVRAQQAGWIDRDAYAYLASQPAEIRVTVGDKR
                     LCIVHGSPWDPHNEYVYPHSPILRKFAEFDADIVVLGHTHYQMAERAGRTLVVNPGST
                     GQPRDPRNDFQLSFAILDSESDEVRFCSFPDPTRACVTQTVATWSV"
     gene            2403..5666
                     /locus_tag="VIM45_00720"
     CDS             2403..5666
                     /locus_tag="VIM45_00720"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY8819839.1"
                     /translation="MERFAEQLADQYEQTLSGYPVSLPRDFRQALAQIEGSLSEEELR
                     QWAEDGAALARHSLRSWEAAGEYFRSSPLLIGRMPFTLVRRWAESGRELAEESATLAS
                     AFFRAGAEGLDKLSVHQMREWAGLGKQLYKGTWKSSSLASQFFEVSPQLIQHLTMTET
                     RSLARFVDTLASRSYELASACLGIAGRVLGGMARSDRGPFLGFAAVIAETSWADARIY
                     FEKGPGLLHHVQAEQRRRFLMLSSAVARRAGRQAYPFFAESASALGQLDQELHGRLLT
                     MSELLAPKSGAAAMEFLKSVPDVLGRIRFHDLEAWHGEGRRLLDQTEDGGLAFFRLES
                     GKGEEVIEALSSRVELSRVGEVLRMYCKALTGTNVSIQAAGALADKGVGWVSSERAST
                     EGTSVFLPAVVEEATDKGENFAVYKVYATHQAGHLEFNSFGFRFERPGAVVAISRIAR
                     AAAGETRAALTDMERYFDLFEDRQLASDLFTVVEDARVDFLIKGEYGGIRGALRRIQS
                     RELGRRSSVQEMPLRKGAVENLIRLSLDGAHTVRWPIADMRMMGNAAGILRQIYHPDA
                     TIEDTAEVTLQLYDLIGQIPNVVQEDLAEEDWSELTEDDISLDMLQTKGQAGQPQPGG
                     EGNIGEPQEGEVPYDSPDAPDFRGDFKPELVQLLMRLRGDAKNQGETDGQFAPLTPEQ
                     LKELMEKSVEIDISAFVEGDLQMSSGMFLANLMKETGTPASEKSKDKGEKGEGEPGEG
                     SEEEGQETQVENPIEYFWYDEWDFRANDYKPRWCRVVQQTLDEGDDKFFEKTLVENSA
                     LVAQTRKQFELMRPELFRKIKRLYDGEELDIDAAIEYVIERRSGHTGDDKVYWRRNKI
                     ERDVAVAFLLDMSASTDEEINRRDRSKGEDDDFDDDPRRYLAWWAQKRAKELQNPVKR
                     IIDLEKESTVLLMEALETIGDRYGIYGFSGYGRDNVEFYVIKDLGEDFSEHIKRRIDK
                     VVPIRSTRMGPAIRHAITKLDQFDAKVKILFLVSDGRPQDHGYGRDRTEKEYAIHDTK
                     MALNEAKRKGIVPFALTVDRAGHDYLKTMCEDIGYEVVADIESLPSRLPTLYRKLTE"
     gene            complement(5722..>6097)
                     /locus_tag="VIM45_00725"
     CDS             complement(5722..>6097)
                     /locus_tag="VIM45_00725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8819840.1"
                     /translation="PTPGIHFNMSGNRFRSLPDFMRSLQTVRSLSPRRVLPGHGAPFT
                     DLAGAVDRTAGVFEQRAKRVLRKLVSGPASAYELALRLYPHLRPSATWIVMAEIIGLL
                     DLLEERGQVAPLHEDTLSYRAT"
BASE COUNT         1235 a         1813 c         1985 g         1064 t
ORIGIN      
        1 cgcgttgacc cccattcggg ccggttgcta agattgaacc gtcgccggca caacaccggc
       61 gattggtgtg tacgcatgat cgtcatcggc ctcaccggcg ggatcgccag cggcaagtcc
      121 accatttcgg gtgcactcgc cgagcatggt gcgaccgtcg tggacgccga caaagtgggt
      181 cacgacgtct accggccagc tacggaaggc tggcagaagg tcgtggacgc cttcggcaag
      241 cagatcctgg cccccgatgg cgagatcgac cggaaggcgc tcggggcgat cgtatttggt
      301 gacccggcgc accgcgagcg gctccaggcg atcgtgtggc cgctcatgaa ggggatgatg
      361 cgccggctga tcgacgattt ccgcaatcag ggcatcgggg tcgccgtgat tgaggccgcg
      421 gtcctgattg aagctgactg ggtcgatctg gtcgaccagg tatgggttgt gaccgtgccc
      481 gttgaggtcg ccgcggcccg cctgatcggc cgcaacggtc tgactcccga tcaggcccgg
      541 gcacgcatct ccgctcagct cagcaacgag gagcgagcgc gacaggcgga cgtggtaatc
      601 gacaacagcg gctcggtcga acaggtcagg agccgtttgg cagagctctg ggacggtctg
      661 gtactcgcgg gtagccgcta agagattcag ccaagcaggc gtgacggagc cggttagata
      721 ctgaccgccg gacgggcggc accctgcaga cgagccaaac ccggatgtag gacccgtaag
      781 ggatggcgtg tggcaatgac aaccgacacc gagcagcagg accattttcg gcagtatgcg
      841 atcgaggagt ataccccgaa gcgcgagcct ttctacgtgg gtatcaatga cgaagagcaa
      901 ctcttccgcg ctgcctttga acagaagatc cccgtgctcc tcaagggtcc gaccggctgc
      961 ggcaaaacgc gattcgttga gtacatggca tacgtgctcg gacgtccgct gaccgtcatc
     1021 aaggaaaaag ccggaagcgg tggcgagcca cagaataacg gagcaggcga cggtcacgga
     1081 ttgtccgtcg tgaccgtcgc atgtcacgaa gacctgacag cgggcgacct cgtcggccgc
     1141 tatctgctcg agggcgaggg cacgcgttgg atggacggcc cgttgacgcg ggcggtcaaa
     1201 cgtggggcca tctgttattt ggacgaagtc gtcgaggcac gcaaagacac gaccgtcctg
     1261 atccacccgc tcaccgacca tcgccgcctg ctgccggtgg agaagctcgg ccaggtgatc
     1321 gaggcttccg agggtttcct gctggtcatc tcttacaacc ccggctacca gagcgcgctc
     1381 aaggacctca agcacagcac ccgccagcgt ttcatcgcgc tcgacttcac ctatccgccg
     1441 cgtgagaccg aagcgaaaat catcgagcac gaggccggca tcgacgcggc gaccgcaatg
     1501 gagctcgcca agctcggcga gaaagtgcgt aacctgcgcg agcacggact gggtgaaggc
     1561 gtaagcaccc gcctgcttat ctacgcgggc aagctcatcg ccaaggggat cacgccgcgc
     1621 cgcgcctgcc aggtgggagt cgtctgggcg ctctccgatg acgccgaggt gcagcgcagc
     1681 atcgaggaag ttgtctcgtc gatctttgag taaccgcaat tgaagatcgg catcgtctct
     1741 gacattcatt gcaatattgc ggggctcgac cgtgcactcg ccgagatggg cgacgtcgac
     1801 gagttgatct gcgccggcga cagcatcttc cagtatcgct tctccaacga ggtcgtgacg
     1861 cgattacggg agcgcggggc gcgggtcatt ctcggtaacc acgaagagac atttttcagc
     1921 tcggccggcg tacgcgccca gcaagcggga tggatcgacc gcgatgctta cgcctatctt
     1981 gcgagccagc cggccgagat tcgggtcacc gttggggata agcggctgtg catcgtacac
     2041 ggcagcccct gggaccctca caatgaatac gtctatccgc acagcccaat actgcggaag
     2101 ttcgcggagt tcgacgcgga tatcgtcgtt ctcggtcaca cgcactacca gatggccgaa
     2161 cgcgccggac gaacgctcgt cgtcaatccc ggctctaccg gtcagccgcg cgacccgcgt
     2221 aacgactttc agttgagctt tgctattctg gatagtgaga gcgacgaggt acggttctgc
     2281 agcttccccg atccgacgcg cgcctgcgta acgcagaccg tagcgacgtg gtcggtttag
     2341 acacggcagc gcccggttag cgtatttggt aatgatcagt cgccatggag taggcaggac
     2401 cagtggaacg cttcgctgaa cagttggccg atcaatacga gcagacgctg agcggctatc
     2461 ccgtctcact gccgcgcgac tttcggcagg cgctggcgca gatcgaaggc tcgctatccg
     2521 aggaagagct gcggcagtgg gcagaagacg gtgccgcgct ggcgcgccat tcgttgcggt
     2581 cctgggaagc ggccggcgag tattttcgct ccagtccgtt gctgatcggg cgcatgccct
     2641 tcacactcgt tcggcgttgg gcagagtcgg gacgtgaatt ggcggaggag tcagctaccc
     2701 tcgcgtcggc gtttttccgc gccggggcag aaggtctcga caagctctcc gtgcaccaga
     2761 tgcgcgaatg ggcaggcctg ggcaagcagc tttacaaagg cacctggaag tcgagctcgc
     2821 tcgcgtcgca gttctttgaa gtcagcccgc aactgattca gcacctgacg atgacggaaa
     2881 cgcggtcgct tgcccgtttc gtcgatacgc tcgcgtctcg ttcgtatgaa ctcgcctcgg
     2941 cgtgtctggg aatagcagga cgtgtgctgg gcggcatggc acgcagcgac cgcggtccgt
     3001 tcctcggctt cgccgccgtc atcgcggaga cgtcctgggc ggatgcacgc atctacttcg
     3061 aaaaaggtcc gggactcctt caccacgtcc aggccgagca acgccggcgc ttcctcatgc
     3121 tgtcatccgc ggtggcgcgc cgagccggcc gccaagcata cccattcttc gccgaaagcg
     3181 ccagcgccct tggccagctc gaccaggaac tgcacggccg gctgctgacc atgtcggagc
     3241 tgcttgctcc taaatcgggt gccgcggcga tggaattctt gaaaagcgtt cccgacgttc
     3301 tcgggcgcat ccgcttccac gacctggaag cctggcacgg cgagggccgc cggctgctgg
     3361 accagaccga agatggcggt cttgccttct ttcggttgga gtccggcaaa ggcgaagaag
     3421 taatcgaggc gctttcgtca cgggtcgagc tctcgcgcgt gggcgaagtg ctccgtatgt
     3481 actgcaaggc gctgaccggt acgaatgtca gcatccaggc cgccggagcg ctggccgaca
     3541 aaggcgtcgg ctgggtgagc tcggaacgcg cttcgaccga gggcacttct gttttcctgc
     3601 ccgccgttgt cgaggaagcc actgataagg gtgagaactt cgcggtttac aaggtttacg
     3661 cgacgcacca ggcgggacac ttggagttta acagcttcgg gttccgcttc gagcgcccag
     3721 gtgccgtcgt cgcaatctct cgcatcgccc gcgccgcggc cggcgagaca cgtgcggcgc
     3781 tgacggacat ggagcgatac ttcgacctct tcgaggaccg ccaactcgcc tctgatctgt
     3841 tcacggttgt cgaggatgct cgcgtcgact ttcttatcaa aggcgaatat gggggcattc
     3901 gcggtgcgtt gcggcgcatt caatcgcggg agctgggcag gcgaagctcc gtgcaagaga
     3961 tgccgctgcg caagggcgcg gtcgaaaacc tcatccgttt gagcctggac ggcgcccaca
     4021 ccgtgcgttg gccaatcgct gacatgcgca tgatgggcaa cgccgccgga atcctccgcc
     4081 agatctacca ccccgacgcg accatcgaag acacggcaga agtcacgttg cagctctatg
     4141 acctcatcgg ccaaattcct aacgtcgtgc aggaggacct ggccgaagag gattggtcag
     4201 agctcaccga ggacgacata tcgctcgata tgctgcagac caaggggcaa gcggggcagc
     4261 cccaacctgg cggcgaaggc aacatcggcg aaccgcagga aggcgaagtc ccgtacgaca
     4321 gcccggatgc cccggacttc cgcggcgact tcaaacccga gctggttcag ctattgatgc
     4381 gtctgcgggg cgatgccaag aaccagggcg agaccgacgg ccagttcgcg ccgctcacgc
     4441 ccgagcagct caaggagctg atggaaaaga gcgtcgagat agatatctcc gctttcgtcg
     4501 agggcgacct gcagatgagc tccggcatgt tcctcgcaaa cctcatgaag gaaaccggaa
     4561 cgcccgcttc agaaaagagc aaggacaagg gcgaaaaagg tgagggggag cccggagaag
     4621 gtagcgagga agaagggcaa gagacgcagg tcgaaaatcc gatcgagtac ttctggtacg
     4681 acgagtggga cttccgcgcc aatgactaca agccgcgctg gtgtcgtgtc gtgcaacaaa
     4741 cgctcgatga gggcgacgac aagttcttcg agaagaccct cgtggaaaat tcggccctcg
     4801 tcgctcagac ccgaaagcag ttcgagctga tgcgtccgga gctgttccgg aagataaagc
     4861 gtctctacga cggcgaagag ctggacatcg acgcggcgat cgaatacgtg atcgagcggc
     4921 gcagcggtca cacgggcgac gataaagtct actggcggcg caacaagatc gagcgggacg
     4981 ttgccgtggc cttcttgctg gacatgagcg cttccaccga cgaggagatc aaccggcgcg
     5041 accgcagcaa gggcgaagac gacgatttcg acgacgatcc gcggcggtac cttgcgtggt
     5101 gggcgcagaa acgggcaaag gagctgcaga acccggtcaa gcgcatcatc gatctcgaga
     5161 aggaaagcac cgtcctgctc atggaggcgc tcgagacgat cggcgaccgc tacggcattt
     5221 acggcttctc agggtacgga cgggataacg tcgagttcta cgtgatcaaa gacctgggcg
     5281 aggacttctc cgaacacatc aaacggcgca tcgataaggt ggtgccgatt cgcagcacgc
     5341 gaatgggacc ggcgatccgc catgcgatca cgaagctcga ccagttcgac gcgaaggtga
     5401 agatcctgtt cctcgtctct gacggccgcc ctcaagacca cgggtacggc cgcgaccgga
     5461 cagagaagga gtacgccatt cacgacacga agatggcgct taacgaggcg aagcgaaaag
     5521 ggatcgtacc gttcgcattg acggtggaca gggccggtca cgactacctg aagacgatgt
     5581 gcgaggacat tggctacgaa gttgtcgcgg acatcgagtc cctccccagc cggctgccga
     5641 cgctctacag gaagttgacg gaataagacg atggcggccg gcgcggcctc gtcggggagt
     5701 tagggggcct gcccgcgttt gctacgtggc ccggtagctc aatgtgtctt catgcagcgg
     5761 cgctacttgg cctcgctctt cgagcaggtc gagtagacca atgatctctg ccatcacgat
     5821 ccaggtggcg gacgggcgta aatgcggata caggcggagc gcaagctcgt aggcgctcgc
     5881 tgggccgctt acgagcttgc gcagaacgcg tttggccctt tgttcgaaga cgccggccgt
     5941 cctatctacg gctccggcca ggtctgtgaa cggcgcgccg tgtccgggca gtacgcgacg
     6001 cggtgagagc gaccgcacgg tttggagcga gcgcatgaaa tcggggaggc tgcggaaacg
     6061 gttgcctgac atgttgaagt ggatgcccgg cgtcggc
//
LOCUS       DATCPE010000021         6855 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U7219
            k141_294628, whole genome shotgun sequence.
ACCESSION   DATCPE010000021 DATCPE010000000
VERSION     DATCPE010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955033
            Sequence Read Archive: SRR1586279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 6855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 16:49:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 640
            CDSs (total)                      :: 631
            Genes (coding)                    :: 627
            CDSs (with protein)               :: 627
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6855
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_294628"
                     /isolate="SMAG_U7219"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..840)
                     /locus_tag="VIM45_00730"
     CDS             complement(<1..840)
                     /locus_tag="VIM45_00730"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY8819841.1"
                     /translation="MQTRAARVITEVPGPASRRLVEAESHSLAPGLQSIGLFSGIALD
                     HGRGALVTDVDGNTFIDFTAGVGVASLGHAHPSYVRALSDQLERISVGSYTTEKRALF
                     LEELRPYLPLGLDRMQFYSGGTEAVEAALRMARSFTGRFEVISFWGGFHGKTGTTLGL
                     SGPDVKQGLGPMHPGVHNVPYPDWARRPFPAVDPVDLGVRCADFLRAYIKNSTAGSLA
                     AIIVEPIQGTAGNVLPPPGFLRSVADIARENGALFIADEMITGFGRTGRMFGCEHFDV
                     VPDA"
     gene            complement(1039..1452)
                     /locus_tag="VIM45_00735"
     CDS             complement(1039..1452)
                     /locus_tag="VIM45_00735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8819842.1"
                     /translation="MNDDSATPGAYNTPITLNIVAAGNGWWVNTGPDPSEIATYIFLD
                     AEEHPELLELPRLSAGADWSAAAEWLADGARIRVNITGPVQTTLDVQVGGPGLEAVRH
                     ARLLYICPTTPELGLSPYLARDRSFAVRLPRLRDA"
     gene            complement(1493..2689)
                     /gene="ispG"
                     /locus_tag="VIM45_00740"
     CDS             complement(1493..2689)
                     /gene="ispG"
                     /locus_tag="VIM45_00740"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015166215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(E)-4-hydroxy-3-methylbut-2-enyl-diphosphate
                     synthase"
                     /protein_id="HEY8819843.1"
                     /translation="MNVDIVCQRRPTREVHAGSAVIGGNAPILVQSMITEETRDVAAC
                     VESIIRLYRAGCELVRVTTPTIADARYLGEIRADLDRRGHKIPLVADVHHQGTDIAVE
                     VARYVDKVRLNPGLFVYRKPRQRAEEYSPSEIQEELDAIEQSLFPVIEVCKQRGIAMR
                     IGVNHGSLAERMLVTYGDTPEGMVESALEYVRICEAHDYRNLVISLKASRVPIMVQAN
                     RLMARRQDELGMGYPLHLGVTEAGDGEYARIKSTAGIATLLSEGIGDTIRVSLAEDPI
                     NELPVCYDILQALGLRRTKVEFIACPSCGRTKFNLPTVFNQVKEATSHLVGLNIAVMG
                     CIVNGPGEMADADYGYVGRGGGQIALYRGREVVKTSIPQEDGVRELVGLIKADGRWLD
                     QPATNQ"
     gene            2757..3338
                     /locus_tag="VIM45_00745"
     CDS             2757..3338
                     /locus_tag="VIM45_00745"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8819844.1"
                     /translation="MHEHAVAADILAHLATEPGREESIGSLATLSGRSAAAIAAACDL
                     LKRWSLIDDSVTGAVRLPPVWSHSDQPVILIVENTVAVAHLVGALLDSEGYRVLIAGT
                     LDIGRRVLESVPIDLVVADSFSDTARDALNRLSVLRDANRSTPVMIFTAHRDLDDERA
                     KTEGFAGVLRKPFDIDDLLARVSSQIMARRTVG"
     gene            complement(3406..4296)
                     /gene="sucD"
                     /locus_tag="VIM45_00750"
     CDS             complement(3406..4296)
                     /gene="sucD"
                     /locus_tag="VIM45_00750"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013538158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEY8819845.1"
                     /translation="MSILVDENTRLLVQGITGGEGSQHARRSIAYGTSVVAGVTPGRG
                     GQKFDDTVPVFNTVHQAVKETGANVSIIYVPAAGAADAILEAADAGLPLVVCITENIP
                     VLDMVKVRRALQGGKTRLIGPNCPGVITPGAKCRVGIMPGQVFAPGSVGVISRSGTLV
                     YEAVAQLTSLGIGQSTCVGVGGDPVIGTRQQEVVEMFNDDPETAALVLIGEIGGSAEQ
                     EAADYIKAHVRKPVIAFIAGSTAPAGRRMGHAGAIISGNSGTAAAKKQALSDAGATIV
                     ESPAEIGATTKRVLRERGLT"
     gene            complement(4333..5502)
                     /gene="sucC"
                     /locus_tag="VIM45_00755"
     CDS             complement(4333..5502)
                     /gene="sucC"
                     /locus_tag="VIM45_00755"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HEY8819846.1"
                     /translation="MKIHEYQAKQILSEFGVPVPKGRVARTPADARQIAEELGGKVVV
                     KAQIHAGGRGKGGGVKLANSPDEAEIVAGQILGMQLVTHQTGPEGQKVKSVLVEEQMQ
                     IERELYLAVLVDGAAGMPVMMASAAGGMDIEEVAAKTPEQIYRVAVDPATGFQPYMAR
                     ELAFALDLSGEQLRSATGLMIGLYNAFLANDASLAEINPMVVTTDGRLLALDAKFNVD
                     DSALYRHKDIADLRDVDEEDPLEVEARELGIGSYIKLDGNIGCIVNGAGLAMATMDTI
                     KLAGGEPANFLDIGTVNDPSRVVNALKVIIEDPAVKAILVNIFGGMARIDIIAQGVIN
                     GYKELGLKLPVVARLDGTNLAEGEKLLTESGVPVIRGRDLGEAAKLAVEAAGKVA"
     gene            complement(5615..6829)
                     /locus_tag="VIM45_00760"
     CDS             complement(5615..6829)
                     /locus_tag="VIM45_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019071896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEY8819847.1"
                     /translation="MRDAVICMPLRTPVGRYGGSLRDVSAETLAGLVIEQVLARSGLP
                     PASVDDSIWGHCYPNGETPAMGRLAPLHAGLPVEVPGFQLDRRCGSGLQAICLAAMEV
                     QTGVADVVLAGGVESMSNAEFYTTNARWGPNRGEIKLMDRLDRGRVTAGTYRYPVDGG
                     MLETAENLRRQYNIPREEQDEYALRSHQRAVAAIESGKFDNEIVPVPVPSRKGEGVLF
                     GRDEHPRADTTLEKLASLRAVRARQDPEATVTAGNASGENDAAACCIVTTPERAAELG
                     LKPMGKLRSWGVAGVHPAYMGIGPVPATKKALERGGLTLGDIDLIELNEAFAAQVLAC
                     TREWGFTEKDFDRTNVNGSGISIGHPVGATGVRIMATMLNEMERRGSQFALETMCIGG
                     GQGIAAVIERVG"
BASE COUNT         1187 a         2178 c         2118 g         1372 t
ORIGIN      
        1 tgcgtcgggc acgacgtcga agtgttcgca gccgaacatg cggccggtgc gaccgaagcc
       61 ggtgatcatt tcgtcggcaa tgaagagggc gccgttctcg cgggcgatgt cggcgaccga
      121 acgcaggaag cccggcggcg gaagcacgtt gccggcagtt ccctggatcg gctcgacgat
      181 tatcgccgcc aagctgccgg ccgtgctgtt cttgatgtac gcgcggagga agtccgcgca
      241 gcgtactcct aggtcaaccg ggtcgacggc tggaaacgga cggcgcgccc agtccggata
      301 gggcacgttg tgtacgccgg gatgcattgg tccgagcccc tgcttgacgt ctggtccgct
      361 caagccgagc gtggtcccgg ttttgccgtg gaagccgccc cagaatgaaa taacctcgaa
      421 gcgcccggtg aacgagcgcg ccatcctcag cgccgcctcg actgcctcgg tcccgccgga
      481 gtagaactgc atccggtcga gtccgagcgg aaggtatggc ctcaactcct ccaaaaagag
      541 cgcccgtttc tcggtggtgt agctcccgac ggagatgcgt tcgagctgat cggacagggc
      601 tcgtacataa gaaggatggg catggccgag cgacgcgacg cccaccccgg cggtgaagtc
      661 gatgaatgtg ttaccgtcca cgtcggtgac caaagcgcca cgcccgtggt ccaaagcaat
      721 gccgctgaaa aggccgattg actggagccc tggcgccaga ctgtggcttt ccgcctctac
      781 caggcgtcgg gaggcgggtc cgggtacctc cgtaatgacc cgtgcggcac gtgtctgcat
      841 gctgctcctt gctgtgtcgg cgggcgatca gtcgcgcgcc gcgggccgga ttgaggttcc
      901 gatcggtcgg gtaaccggcc tcgatattat gaactgacga tcccaagtgt acaaccacgg
      961 atccgctggt acataagaaa attgagcctt ttctcggtga cgccgccgca tgcgtacgcg
     1021 ctcaccgaat gcagatgatc acgcatcgcg aaggcgtggc aatctgaccg cgaagctacg
     1081 gtcgcgcgcg aggtacgggc tcagaccgag ctcgggggtg gtagggcaga tatacaacag
     1141 ccgcgcgtgt ctgactgctt cgagcccggg accaccgacc tggacgtcga gggttgtctg
     1201 cactggtcca gtgatgttga cgcggatcct tgcgccgtcg gcgagccatt ccgcggcggc
     1261 cgaccagtcc gcgccggcgg aaagacgggg cagttccagt agctcggggt gctcctcggc
     1321 gtctaagaat atgtaggtgg cgatctccga tggatcggga ccggtgttca cccaccaacc
     1381 gttgccggct gccacgatat tcaacgtgat gggtgtgttg taggcgccgg gcgtggcgct
     1441 atcgtcgttc atgcatgcgc atccgagaaa ctgtgtgtaa gagacgtcgg ctctattggt
     1501 tggtggcggg ctgatcgagc cagcggccat cggccttgat cagtccaacc agttctcgaa
     1561 cgccgtcttc ctgcgggata ctcgttttga ctacctcgcg accccgatac agcgcgatct
     1621 gaccgccgcc gcgccccacg tagccgtagt cggcgtccgc catctcgccg gggccgttga
     1681 cgatacagcc catgacggcg atattgagcc cgaccaggtg gctggtcgcc tctttcacct
     1741 ggttgaacac ggtcggcagg ttgaactttg tgcggccgca cgacgggcac gcgatgaact
     1801 ccaccttggt acggcgaagg ccgagcgcct gcaagatgtc gtagcaaacc ggcaactcgt
     1861 tgatcgggtc ctccgcaagt gagacgcgga tcgtgtcgcc gatcccctct gataagagcg
     1921 ttgcgatgcc cgcagtcgat ttaatcctgg cgtattcccc gtcgccagcc tcggtgacgc
     1981 ccaggtgcag agggtaaccc atgcccagct catcctgccg ccgggccata agtcgattgg
     2041 cctggaccat gatcggcact cgcgatgcct ttagcgagat aacgaggttg cggtagtcgt
     2101 gcgcttcgca aatgcgaacg tactcgagcg ccgactcaac cataccttcc ggggtgtcgc
     2161 cgtaggtcac gagcattcgc tcggcaagtg aaccgtgatt gacgccgatg cgcatagcga
     2221 tgccgcgctg tttgcagact tcgataacgg ggaacagact ctgttcaatg gcgtcgagtt
     2281 cctcctggat ctcggacggc gaatactctt ctgcccgctg gcgcggcttg cggtagacga
     2341 agagtcccgg gttcaaccgg actttgtcga catacctggc cacttcgaca gcgatatccg
     2401 tgccctggtg gtggacgtcg gcgaccaacg gaatcttgtg accgcgccgg tcgaggtcgg
     2461 cgcggatctc gcccaggtat cgggcgtcgg ctatcgtcgg cgtcgtgacc ctgacaagct
     2521 cacagcccgc ccgatagagc cggataatcg actccacaca ggcggcgaca tcgcgtgtct
     2581 cttcggtgat catcgactga acgaggatcg gtgcgttgcc gccgatgacc gccgagccgg
     2641 cgtgtacctc gcgggtgggt cttcgttgac atacaatgtc cacgttcatt gtctcccggg
     2701 ttgcggcggg cagaatgatg tactctacga actattgtgc tacaaccaac tgaatcatgc
     2761 acgaacatgc ggtcgcagcg gatattcttg cgcacctggc tacagagccc ggtcgcgaag
     2821 agtcaatcgg atcgcttgcc actctcagcg gcaggtcagc ggcagctatc gccgcagcgt
     2881 gcgaccttct caaacgctgg tcgttgattg atgacagtgt aaccggagct gtgcgtcttc
     2941 ccccggtatg gtctcattct gaccagccgg tcattttgat cgtcgaaaac accgttgccg
     3001 ttgcgcacct cgtcggtgct ctgctcgata gtgaggggta ccgagtactg attgcgggaa
     3061 cgctcgatat aggtcgccgt gtcctcgaat ccgttccaat cgaccttgtt gtcgccgaca
     3121 gcttttcgga tactgcgcgc gatgcactta acaggctttc ggttctgcgc gatgcgaacc
     3181 gctcaactcc ggtgatgata ttcaccgcgc atagagatct ggatgacgag cgcgccaaaa
     3241 cggaaggctt tgccggcgtc ttgcggaagc cgtttgatat tgacgatctc ctcgcgcggg
     3301 tgtcgtcgca gatcatggcg cggcgcacgg tcggttgacc tcagcgttcg gctgccgcag
     3361 tcagaattcg ggtaaagagg cgcagttcgg ggatctcggg caggctcagg taaggccgcg
     3421 ctcccgcagc acccgcttcg tcgtcgcccc aatctccgcc gggctctcga cgatcgtggc
     3481 gccggcgtcg ctcagcgcct gcttctttgc agctgccgta cctgagttac cagagatgat
     3541 cgcgcccgcg tgacccatgc gacggccggc aggcgccgtg ctgccggcga taaacgcgat
     3601 cacgggtttg cggacgtgcg ccttgatgta atcggccgcc tcttgttcgg cgctgccgcc
     3661 gatctcgccg atgagcacca gcgccgcggt ttcgggatcg tcgttgaaca tctcaacgac
     3721 ctcttgctgt cgagttccga tcaccgggtc gccgccgacg ccgacacaag tggactggcc
     3781 gatgcccagg ctggtgagct gcgctacggc ttcgtagacg agcgtgcccg agcgcgagat
     3841 gacgccgacg cttcccggag cgaacacctg gccgggcatt atgccgacgc ggcacttggc
     3901 gccgggcgtg ataacgcccg gacaattggg gccgatgagc cgcgtcttcc caccctggag
     3961 cgctcgccgg actttcacca tgtcgagaac ggggatgttt tcggtgatgc acacgacgag
     4021 gggaaggcca gcgtccgcgg cttccagaat ggcgtccgcg gcgcctgcag ccggcacgta
     4081 gataatgctg acgttggcgc cggtctcttt caccgcctgg tgcacggtat tgaagaccgg
     4141 cacggtgtcg tcgaacttct ggcccccgcg gcctggtgtc accccggcga cgacactcgt
     4201 gccgtaggca atgctgcggc gggcgtgctg gctgccttcg ccgccggtaa tcccttgcac
     4261 cagcaggcga gtgttctcgt caaccaggat gctcaatgca gctctcctcg tatcgatggt
     4321 cggattgtgc gttcaggcga ccttgcccgc tgcctcgaca gccagcttcg ccgcctcgcc
     4381 gagatccctt cctcggatga ccggaacgcc ggattcggtc agcagcttct cgccttcagc
     4441 gaggttggtg ccgtcgagcc gcgccacgac gggcagcttt aagccaagtt ccttgtatcc
     4501 gttgatcacg ccctgggcga tgatgtcgat tcgcgccatt ccaccgaaga tgttcaccag
     4561 gatcgctttg acagcggggt cttcgatgat cactttaagc gcgttcacca cacgacttgg
     4621 gtcgttgaca gttccgatgt caagaaagtt tgcaggctca ccgccggcca gtttgattgt
     4681 gtccatcgtc gccatggcga gaccggcgcc gttgacgatg cagccaatgt tgccatccag
     4741 cttgatgtag ctgccgatac cgagctctct tgcttccacc tcgagtggat cttcctcgtc
     4801 gacgtcgcgc aggtcggcga tatctttgtg gcgatagagg gcgctgtcgt caacattgaa
     4861 cttggcgtca agtgcgagca gccgtccatc tgtcgtaaca accatcgggt tgatctcggc
     4921 cagtgaggcg tcattggcca agaacgcgtt gtagagtccg atcatcaatc ctgtcgcact
     4981 acgcaactgc tccccgctga ggtcaagcgc gaacgccagc tcgcgcgcca tgtacggctg
     5041 aaagccggtg gctggatcga cagctacccg gtaaatctgt tccggcgttt tcgccgcgac
     5101 ctcctcgatg tccatgccgc ccgcggcgct cgccatcatg accggcatac cggccgcgcc
     5161 gtcgaccagc acggcgaggt atagctcgcg ctcgatctgc atttgctctt ccacgagcac
     5221 gctcttgacc ttttggccct ccgggccggt ctggtgggtg acgagctgca tgccaaggat
     5281 ctggccggca actatctctg cttcatcggg actgttggcg agcttgactc ctccgccttt
     5341 gccacggccg ccggcgtgaa tctgcgcctt gaccaccacc ttgccaccca gctcttcggc
     5401 gatctggcga gcgtcagccg gcgtgcgcgc aacgcgcccc ttgggtaccg gcacgccgaa
     5461 ctccgacagg atctgcttcg cctggtactc gtgaatcttc atcaaagact ccaaccgtgc
     5521 ggcatcaagg gcagcatgac ataacgtcaa caataaggga gtcgggggag aaagtccaaa
     5581 atcgactcta gcacagggga tgctgggacg ttccctatcc cactcgctcg atcacggcgg
     5641 cgattccctg gccaccaccg atgcacatcg tttcgagcgc aaactgcgat ccgcggcgct
     5701 ccatttcgtt gagcatcgtc gccatgatgc ggactcccgt cgcgccgacg gggtggccga
     5761 ttgagatgcc cgatccattg acgtttgtcc gatcgaagtc cttttcggtg aagccccact
     5821 ctcgcgtgca ggccagcacc tgggcggcaa acgcctcgtt gagctcgatc aaatcgatat
     5881 cgccaagcgt tagcccgccg cgttccagcg cttttttggt cgccggcacc gggccgatgc
     5941 ccatgtacgc cggatgaaca ccagcaacac cccacgagcg gagcttgccc atcggcttca
     6001 atcccagttc ggccgcccgc tcgggcgtgg tgacgatgca gcaggcagcc gcgtcgtttt
     6061 cgccgctcgc attgccggct gtcacggttg cctccggatc ctgccgggct cgcactgccc
     6121 gaagtgaggc cagcttttcg agggtcgtgt cggcgcgggg atgctcgtca cgaccaaaca
     6181 ggacgccctc tccctttctg gaagggacgg gcaccggcac gatctcgttg tcaaactttc
     6241 cagattcgat cgcggcgaca gcgcgctggt ggctgcgcag cgcgtactcg tcctgctctt
     6301 cgcgcgggat attgtactgg cggcgcaggt tttcggccgt ctccagcatg ccgccgtcca
     6361 ccggatatcg ataggtgccc gcggtcacgc gaccgcgatc gaggcggtcc atcagcttga
     6421 tctcaccccg gtttggcccc cagcgcgcgt tggtcgtata gaactcggcg ttgctcatgc
     6481 tttccacgcc gccggcaagt acgacgtccg ccacgcccgt ctgcacctcc atcgctgcga
     6541 ggcatatcgc ctgcaacccg gagccacagc ggcgatccag ctgaaagccc gggacctcca
     6601 ccggcaggcc ggcgtgcagc ggcgccagcc gccccatggc cggtgtttcc ccgtttgggt
     6661 agcagtgccc ccaaatgctg tcgtccacac tggcgggagg aagcccagaa cgcgccagga
     6721 cttgctcaat caccagtccc gccagggttt cggccgaaac gtcgcgcagg ctgccgccgt
     6781 agcggccgac gggggtgcgg agcggcatgc aaatcacggc gtctcgcatt gggcttctcc
     6841 tttaatatct tttgg
//
LOCUS       DATCPE010000022         5274 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U7219
            k141_456960, whole genome shotgun sequence.
ACCESSION   DATCPE010000022 DATCPE010000000
VERSION     DATCPE010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955033
            Sequence Read Archive: SRR1586279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 5274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 16:49:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 640
            CDSs (total)                      :: 631
            Genes (coding)                    :: 627
            CDSs (with protein)               :: 627
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5274
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456960"
                     /isolate="SMAG_U7219"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..405
                     /locus_tag="VIM45_00765"
     CDS             <1..405
                     /locus_tag="VIM45_00765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HEY8819848.1"
                     /translation="VTEGDIINGQVEHSVLSPGVYIEDGAVVRDSILFDDCYVERGAV
                     IERAILDKGVYIGEGCRVGTGEDFSSNRARPDLLWSGISLVGKRTRLGAGLAVGRNCI
                     IGPNVREKDIATNWLPSGETIRAPRPTGVPSV"
     gene            427..2118
                     /locus_tag="VIM45_00770"
     CDS             427..2118
                     /locus_tag="VIM45_00770"
                     /inference="COORDINATES: protein motif:HMM:NF015052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein
                     domain-containing protein"
                     /protein_id="HEY8819849.1"
                     /translation="MPERAGAFAFVLHSHLPYCRRAGRWPHGEEWLHEAASETYLPLL
                     EGLNRLRNEGTKFQLTIGITPVLCEQLADRDVRDNLADFMEDKEQRARSDVGRFEREG
                     DGRRAEGAAWYLEHFISLRSLYESMDRDIVGGFKRLQDDGCIEIATSAATHGYLPLME
                     RDSTIHGQLAVGVQSYKRHFGRDPRSCWLPECAYRPAYERVVGDQRYVKPGIESFLSE
                     LGLRLFFAETHTVEGGAPVGKAVGDAVGPYGSVPRRYVVPIPNYDSPSHRTTYLPYWV
                     SKPEVAVLGRNNRTGLQVWSASHGYPGEADYREFHKRDGVSGLQYWRVTGPEVDLGNK
                     DVWNPSWARDRVAGHADHFAHLVESLLSEFHATSGKTGIISAAYDTELFGHWWFEGVD
                     WLISVLRKLSGSPNVETCTAGQFVAAHSPEEVLALPESSWGQAGNHFVWLNADTEWMW
                     PVIHAAERRMERLVELHPDAGGQLLAVLSQCARELLLLESSDWPFLVTTGQAREYAIQ
                     RFDDHVERFRRLAEVAEGGRPTSDDIALTNELWELDKLFPTIDYRVFSRRDLVWD"
     gene            2173..2592
                     /locus_tag="VIM45_00775"
     CDS             2173..2592
                     /locus_tag="VIM45_00775"
                     /inference="COORDINATES: protein motif:HMM:NF021486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2203 domain-containing protein"
                     /protein_id="HEY8819850.1"
                     /translation="MPKYWTLTEAKAALPEAVALIEQLRDLIRRGRSGAAGRERRHGL
                     GGPRGARSGNGRGPSSNGHGSSEPAGQLDVQARQLMERIQAMGFQVKDVEAGLIDFPC
                     MRNGEEALLCYRLGEPDIQFWHDLESGFAGRRPVEEL"
     gene            2606..3058
                     /locus_tag="VIM45_00780"
     CDS             2606..3058
                     /locus_tag="VIM45_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8819851.1"
                     /translation="MAASVLLLLASACGTGGHHAPPPQATSLRPTGALDPTPATGAIA
                     PATSVTGSATGRPSVTVVAVAGTPGLITLTPAGAKPDALSVQSGRTITWQNDDTNRHH
                     VVSDEPGLFDTGDIAPGSSVTVTVTVIGTHDWHDAVTPRLKGTLRVLK"
     gene            complement(3076..3972)
                     /locus_tag="VIM45_00785"
     CDS             complement(3076..3972)
                     /locus_tag="VIM45_00785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8819852.1"
                     /translation="MICEELRADADALALGALPEKEASQAELHLASCDDCKAEYEAAL
                     RVAGQLPLSAPLVEAPWALRAAVFGAINSDTAGDRVSRSGAGVTGTPVGSRLQTTTHA
                     AVPGGRNALRSASVARFRRVAFYLPTLAAAAMFIAIIGLGVWTLRLHNQVQDLKKSDQ
                     AAARLRISEPALARADTDALLLLSAPGTIKAPLTAGAAAPNANGAVFWSREQQRCVIF
                     ARELTPLRGGSEYHVWFSAGAAYWDGGPLIPDTTGVAEMVVSTERWHPGESYIINIVI
                     QPVPDDGSRRPVLSASIEQDVE"
     gene            complement(3972..4565)
                     /locus_tag="VIM45_00790"
     CDS             complement(3972..4565)
                     /locus_tag="VIM45_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY8819853.1"
                     /translation="MTARRGSLADALTDDALVDRVVARDEEAFAELYERYSDLVYSVG
                     LRVLADPTSALDVAQDVFIRFWRNPGSFDPQRGRFMPWLLSVTRNRAVDEVRSRGRRR
                     LREIAPVEGADDPVDVAAPDPAWMAVLASEQRSVRAALATLPPEQREAIELAYFGGLT
                     QQEIAERVATPLGTIKTRVRLGMKKLRIILRDETEAE"
     gene            4785..>5274
                     /locus_tag="VIM45_00795"
     CDS             4785..>5274
                     /locus_tag="VIM45_00795"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEY8819854.1"
                     /translation="MKAYRLILPVVALTAVALAIACTQKTHPATLGSNVSQPPAATGA
                     SPASAAQGTSAAAPSPALTTTTSKNDATLTSIPDIVKALRPSVVRVRTDASQVGVFGN
                     LQKASGTGTGIILDADGHILTNNHVVTLGTNQPAQKFTVDLADGESLDAQLVGREPNA
                     DLA"
BASE COUNT         1022 a         1636 c         1642 g          974 t
ORIGIN      
        1 gtgaccgaag gcgacattat caatggccag gtcgaacatt cagtgttgtc gcccggcgta
       61 tacatcgaag acggcgccgt ggtgcgcgac tcgatcctct tcgatgactg ttacgtggaa
      121 cgtggtgctg tgatcgagcg ggcgatcctc gacaagggag tctatattgg cgaaggctgt
      181 cgtgtcggca ccggcgagga tttttcgtcc aaccgggcac gccccgacct gttgtggagc
      241 ggaattagcc tggtaggcaa acggacacgc ctgggcgccg gtcttgcggt cggacgcaac
      301 tgcatcatcg gtccgaacgt gcgcgaaaag gacatcgcga ccaactggct cccaagcggc
      361 gaaacgattc gggccccgcg accaaccgga gttccctcgg tctgatcatc gtaaaggacc
      421 atcactgtgc ctgagcgagc cggcgcgttt gcattcgtgc tgcatagcca ccttccctat
      481 tgccgtcgtg ccggtcggtg gccgcacggc gaagaatggc tgcatgaggc cgcttccgaa
      541 acgtacttgc cgctgctgga ggggctgaac cgtttacgca acgaagggac gaagtttcag
      601 ctcacgatcg gcatcacgcc cgttctatgc gaacaactcg ccgatcgcga tgtacgcgac
      661 aacctggccg atttcatgga ggacaaagag cagcgggctc ggtctgacgt cgggcgcttc
      721 gagcgcgaag gggacggccg gcgcgcggaa ggggcggcct ggtatctgga gcacttcatc
      781 tctcttcgga gcctctatga aagcatggat cgcgacatcg tgggcggttt caagcgcctg
      841 caggacgatg gctgcataga gatagcgacc agcgccgcca cgcacggcta cctgccgctc
      901 atggagcgtg attcgaccat ccatggtcaa ctggctgtgg gcgtgcaatc gtacaagcgc
      961 cattttggcc gcgatcctcg ctcgtgttgg ctccccgagt gcgcctaccg cccggcgtac
     1021 gagcgcgttg tgggggatca gcgttacgtc aagcccggaa tcgagagttt tctgtcggag
     1081 cttgggctac ggctcttctt tgcagagact cataccgtcg agggtggcgc tccggtgggc
     1141 aaggcggtcg gcgacgccgt tggcccgtac ggaagcgtcc cccgtcgcta tgtggtgccg
     1201 attcccaatt acgactcgcc gagccaccgc acgacctacc tgccgtattg ggtttcaaag
     1261 ccggaagtcg ccgtgttggg ccgcaacaac cgcacgggac tgcaggtctg gtcggcgtct
     1321 cacggatatc cgggcgaagc ggattaccgc gagttccaca aacgcgacgg cgtatcgggg
     1381 ctgcaatatt ggcgtgttac cgggccggaa gtcgacctcg gcaacaagga cgtctggaac
     1441 ccatcatggg cgcgggaccg ggtggccggg cacgcggacc acttcgcaca tttggtcgaa
     1501 tcgctgctta gcgagtttca tgcgacgagt gggaagaccg gcataatctc ggccgcatac
     1561 gacacggagc tgtttgggca ctggtggttt gagggcgtcg attggctgat ctccgtacta
     1621 cgaaagctat ccggaagtcc gaatgtcgag acctgcaccg ccggccaatt cgtggcggcg
     1681 cactcccccg aagaggtttt ggccttaccg gaaagctcat gggggcaggc cggaaaccac
     1741 ttcgtctggc taaatgctga cacagagtgg atgtggcccg ttattcatgc ggcggagcgt
     1801 cggatggaac ggctcgtgga actgcaccct gacgccggcg gtcaactgct cgcggtgctg
     1861 agccagtgcg cccgagagct gctgctgctg gaatccagcg actggccgtt cttggtcacg
     1921 acaggccagg cgcgcgagta tgcgatacag cgctttgacg accacgtgga gcggttccga
     1981 cggttggctg aggttgcgga gggtggccgg cctacctccg acgacatcgc gttgaccaac
     2041 gagttgtggg aactcgacaa gctgttcccc actatcgatt accgtgtctt cagccggcgg
     2101 gatctcgttt gggattgatt cttgtctctc caaagtcgcc cgcgctcacg ttcccgctgg
     2161 ccggaggcgc caatgccgaa gtactggacg ttgaccgagg caaaggcagc gctgcccgag
     2221 gcggttgcct tgatcgaaca gttgcgagat ctgatccgcc gcggacgcag tggagccgcc
     2281 ggacgcgagc gccggcacgg tctcggaggc ccgaggggcg cccggtccgg caacggtcgc
     2341 ggtccttcga gcaatggaca cggatcaagt gaacctgctg ggcaactcga tgtacaggcg
     2401 cgacagctca tggagcggat ccaggcgatg gggtttcagg ttaaggacgt cgaagccggc
     2461 ctcatcgact ttccctgtat gcgaaacggc gaagaagcgc tgctctgcta ccgcctgggc
     2521 gagcccgaca ttcagttctg gcacgacctc gagagcggct tcgcgggaag gcgcccggtc
     2581 gaagaactgt agcccggtgt tgcaggtggc cgcgtcggtg ctgctgcttc tggcatccgc
     2641 gtgcgggacg ggcggccacc acgcgccgcc gccacaggcg acgtcactgc gaccgacagg
     2701 tgcgctcgat ccgactcccg ccacaggcgc gattgcgccc gcaacgtctg tcacaggaag
     2761 cgcaacgggg cgtccctcgg tgaccgtcgt cgcggtcgcc ggaacaccgg gattgataac
     2821 tctcacgcct gccggtgcga agcccgatgc tctgtcggtt cagtccggac gcacgataac
     2881 ctggcagaac gacgacacga accggcatca cgttgtttcg gacgaacccg gtctgttcga
     2941 cacgggcgac atcgcgcccg ggtcatctgt gactgtcacc gtgaccgtaa ttggcactca
     3001 cgactggcat gatgcggtga cgccccgtct aaaagggacc ctccgcgtgt tgaagtgagc
     3061 aggctggcga gggagctatt cgacgtcttg ttcgatgctg gccgacagaa ccggccgtct
     3121 tgaaccgtca tcgggtacag gctgaatcac aatattaatg atgtacgatt cgccgggatg
     3181 ccacctttcg gtagaaacca ccatctccgc cacgcccgtc gtgtctggaa taagcggtcc
     3241 gccgtcccag taggccgcac ccgcgctgaa ccaaacgtgg tattcactcc cgccacgcag
     3301 cggtgtgagt tcccgcgcga atatcacgca acgctgctgc tcgcggctcc agaagacggc
     3361 gccattcgcg ttgggcgccg cggcgcccgc ggtgagcgga gccttaatcg tccccggagc
     3421 agacagcaac aacagtgcat cggtgtccgc tcgggcaagc gcaggctctg aaatcctgag
     3481 ccgcgcggcc gcctgatctg acttcttgag gtcctgcacc tggttatgaa gccgcagcgt
     3541 ccaaacgccg agcccaatga tcgcgatgaa catcgccgcc gcggccagcg tcggcaaata
     3601 aaaagcgaca cggcgaaagc gcgctacgct tgcgctgcgt aacgcattac gcccgccggg
     3661 gactgcagca tgtgtcgtgg tttgtaagcg gctgccaacg ggcgttccgg tcacgccggc
     3721 accagaccgc gagactcgat ctccagccgt gtccgagttg attgcaccga acacggcggc
     3781 tcgcagggcc cagggagcct ccactagcgg ggcgctgagc ggtaactggc ccgccacccg
     3841 gagcgcggct tcgtactcgg ctttgcagtc atcacacgat gcgagatgca gttcggcctg
     3901 cgacgcttct ttttcaggaa gcgctccaag tgccaaagca tcggcatccg cgcgaagctc
     3961 ttcgcagatc atcactcggc ctcagtctcg tcccgcagga taatgcgcag cttcttcatg
     4021 cccagcctta cccgcgtctt aatcgtgcct aacggcgtcg ctacccgttc cgcgatctct
     4081 tgttgcgtta ggccaccaaa ataagcaagt tctatcgcct cgcgttgctc ggggggcagc
     4141 gtcgccaggg ccgctcggac gctgcgctgc tccgatgcaa gcactgccat ccatgccgga
     4201 tctggagcag caacgtcaac cgggtcgtct gcaccttcga cgggcgctat ctcgcgcagc
     4261 cgtcgccggc cgcgcgaacg gacttcatcg acggctcgat ttcgggtgac gctcaacagc
     4321 cagggcatga accggcctcg ctgtggatcg aaactgccag ggttacgcca gaaccgaatg
     4381 aagacatctt gggcgacatc taatgcagac gtcggatcgg ctaaaacgcg caacccgacc
     4441 gagtacacga ggtccgaata gcgttcgtac aattcggcga aagcttcttc gtcgcgcgcc
     4501 acaacccggt cgactaatgc gtcgtccgta agggcatcag ccaggctgcc ccttcgcgcg
     4561 gtcaccgcgc tacatcccta tgtctatacg acgaaggagc gtgatcggat tgcaaaccct
     4621 atctccgaat ttccctgtga ctgctgcatt gtatcaatta ctccgccgct gtgtttcaca
     4681 gtctgttagc aggagattaa tcgggcgtgt gtatgcggac gctacaaacc cacgtaccgt
     4741 ttagttagag cactcaactc gctacaacaa ggagaagaac agcaatgaaa gcctaccggc
     4801 tcatattacc cgtggtcgcg ctgaccgcgg tagctctggc gattgcctgc acgcaaaaga
     4861 cgcacccggc cacgcttggc agcaatgtca gtcagccacc cgctgcaacg ggggcctcgc
     4921 cggcgtctgc ggcgcagggg accagtgccg cagcgccaag ccctgctcta acgacgacaa
     4981 cgagcaagaa tgacgccacg cttacctcaa ttccggacat cgtgaaggcg ttgcgaccga
     5041 gcgtggtgcg agtccgaacc gatgcatccc aggtcggagt cttcggcaat ctacaaaagg
     5101 cttcgggaac cggcaccggc ataattctcg atgccgacgg acacattctc accaacaacc
     5161 acgtcgtcac gctgggcacg aatcagccgg cacaaaagtt taccgttgac ctcgcggacg
     5221 gcgagtcgct tgacgcgcag ctcgtggggc gggaaccgaa cgcggacctc gcga
//
LOCUS       DATCPE010000023         3043 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U7219
            k141_3226, whole genome shotgun sequence.
ACCESSION   DATCPE010000023 DATCPE010000000
VERSION     DATCPE010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955033
            Sequence Read Archive: SRR1586279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 16:49:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 640
            CDSs (total)                      :: 631
            Genes (coding)                    :: 627
            CDSs (with protein)               :: 627
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3043
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3226"
                     /isolate="SMAG_U7219"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1643)
                     /locus_tag="VIM45_00800"
     CDS             complement(<1..1643)
                     /locus_tag="VIM45_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011929022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HEY8819855.1"
                     /translation="MGIVWIVPIVGAIAVLFAVWLAHDVLVRDKGTPGMQDIADMIFE
                     GAMAFLRRQYQAITIMSVVVAVVIGLIVWGTASPVRDPRSIGEAGSIIGAGGGAGSVG
                     AGEQGILAGVAFLVGAFCSALAGYIGMYISVRSNLRTAAAAQVNLRAAITVALRGGAV
                     SGFLVTALSLLGVSSIFGIYSKVLNHPLGSTPFLIVGFGFGASFVALFAQLGGGIYTK
                     AADVGADLVGKIEAGIPEDDPRNAAVVADLVGDNVGDCAGRGADLFESMSAENIGAMI
                     LGVAIFKATGHVQWIFFPLVLRSFGILATIAGLLSVPYFTREDGNQDPMQPLNGGYYV
                     CSILSVVGLAIATRFMLGSQWYWYFYCGLVGIATGIAFVYITQYYTAGAWRPVKEIAA
                     ASRTGPATNIIIGTAVGFETTAVTALVIGTALVTSFILGAHAGIAPSVAGQKLDTTSM
                     GIFGTAVATMGMLMSAAYILAMDTFGPITDNAGGITEMSGASESARHITDALDTVGNT
                     TKALTKGYAVASAGLAAFLLFSAYLDKVKEVLSSQAVAKG"
     gene            complement(1870..2526)
                     /locus_tag="VIM45_00805"
     CDS             complement(1870..2526)
                     /locus_tag="VIM45_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011929038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase"
                     /protein_id="HEY8819856.1"
                     /translation="MPDELASQLDTIMEGVRARLAAKHAAREQALPLCREVIRLSANG
                     IRAVHRDEFGPAQNMLDKATALLRQIRSALADHGDIFHAGFVHDAQKEHAEAALTLAL
                     VHGDQLPGPDDLAVENAAYLNGLGEAVGELRRYLLDSLRDGDVARCERYLRAMDDIYD
                     VLVTVDYPDAMTGGLRRTTDVTRGILERTRGDLTMAARQSDLERRLAAFESRLPAQAK
                     "
     gene            complement(2596..2937)
                     /locus_tag="VIM45_00810"
     CDS             complement(2596..2937)
                     /locus_tag="VIM45_00810"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEY8819857.1"
                     /translation="MPIYEYRCRECGRLSSVFARTVSSPIEPVCGHCHGKRMERAVSK
                     FAYHRSEQQILEDYGVPEPGSGDSYKDPRQIGRWVEKRFDEMGMELPSEARQMIDAAR
                     EGEFPEPVKDL"
BASE COUNT          605 a          970 c          902 g          566 t
ORIGIN      
        1 ccttttgcga ctgcctggct gctcagcacc tctttgactt tatccaggta agcgctgaac
       61 agcaggaagg cggcaagacc ggctgatgcc accgcataac ccttcgtgag cgccttggtc
      121 gtgttgccga ctgtatccag cgcatcggtg atgtggcgcg ccgattccga ggcgcctgac
      181 atctcggtga taccgccagc gttgtcggtg attggcccga acgtatccat tgccaggatg
      241 taggcagcgg acatcaacat gcccatcgtc gcaactgccg tgccgaatat ccccatgctg
      301 gtcgtatcta gcttctggcc ggctaccgaa ggtgcgatgc ccgcgtgggc gcccagaatg
      361 aacgacgtta ccaacgcggt gccgatgacc agcgccgtga ccgcggtggt ttcgaatccc
      421 accgcggtgc cgatgatgat gttggtcgcc ggccccgttc gcgaagccgc cgcgatctct
      481 ttcaccgggc gccatgcacc ggcggtgtag tactgggtga tgtatacgaa tgcgataccg
      541 gtcgcgatac caacgaggcc gcagtagaaa taccaatacc actggctgcc caacatgaac
      601 ctggtggcaa tggccaatcc cacgaccgat aggatggaac agacgtaata accgccgtta
      661 agcggctgca tcgggtcctg gttaccgtcc tcgcgcgtga agtacggcac cgatagtagt
      721 ccggcgatcg tcgcgaggat accgaacgac cgcaggacga gcgggaagaa gatccattgc
      781 acgtggccgg tcgccttgaa gatggccacg ccgaggatca tcgcaccaat attctcggca
      841 gacatcgact caaacaggtc ggcgccgcgg ccggcgcagt cgccgacgtt gtcacccacc
      901 aggtcggcga cgacggccgc gttgcgcggg tcgtcctcag gaatgccggc ttcgattttg
      961 ccaacgaggt ctgcgccaac gtcggccgcc tttgtataga ttccaccgcc gagttgcgca
     1021 aacaacgcca cgaagctggc gccaaagccg aaaccgacga tcagaaaggg cgtggagccg
     1081 agcggatggt tcaacacctt gctgtaaatg ccgaagatgc tcgaaacgcc cagcagcgat
     1141 agggccgtca ccaaaaaccc cgataccgca ccgccgcgaa gcgcaacagt gatcgccgcc
     1201 cgaaggttga cctgcgcggc cgccgccgtt cgaaggttgg aacgaacaga gatatacatg
     1261 ccaatgtatc cggcgagcgc cgagcagaac gccccgacca ggaacgcgac tcccgccagg
     1321 atgccctgtt cgcccgcacc aactgatccg gcgccgcccc cggcgccgat aatcgatccg
     1381 gcttccccta tgctgcgcgg atcccgcacc ggggaagctg tgccccagac gatcaggccg
     1441 atcacgacgg ccacgacaac cgacatgatg gtgatcgcct gatactgccg cctgaggaac
     1501 gccattgcgc cctcaaagat catgtcggcg atgtcttgca tccctggcgt gcccttgtca
     1561 cgcacaagca cgtcgtgcgc gagccagaca gcgaacagga ccgcgatggc accgacgatg
     1621 ggtacgatcc aaacgatacc cacgtagccc tctccttctt ctgtggctga ttgccggccc
     1681 cgcagacgac gaaacgagcg taaaggcccg ctcgaaaagc tgtggaagct ccgtctacgg
     1741 ataccggtcc ggattggaat ctaacagtcc acatagtgac gatcaagcgt gtaaacgagt
     1801 gattagttag cctgagcagg caattcggca tcagatgggc cggcggaagg cgcacagtag
     1861 cgacgcgaat tactttgcct gtgcgggaag ccgagactcg aatgccgcga ggcgccgttc
     1921 gagatcgctt tgtcgcgccg ccatcgtcag gtcgcctcgc gtccgctcca ggatgccgcg
     1981 ggttacgtcc gtcgttctgc gcagtccacc ggtcattgca tcggggtagt cgacggtgac
     2041 gagtacatcg taaatatcgt ccatcgcacg cagataacgc tcacaccgcg ccacgtcacc
     2101 atcccgcaga ctgtcgagca gatatcggcg tagttcgccg acggcttcgc caagaccgtt
     2161 cagataggcc gcgttttcca ccgcgaggtc gtcggggccg ggcaactggt caccgtgcac
     2221 gagcgcgagt gtgagcgccg cctcggcatg ctccttctgc gcgtcgtgaa caaatccggc
     2281 gtgaaagatg tcgccatggt ctgcgagggc actccggatc tggcgaagca gcgccgtcgc
     2341 cttgtccagc atgttttgtg ccggcccaaa ttcgtctcga tgaacggcgc ggattccgtt
     2401 ggcggagagc ctgatgactt cccgacagag tggcagcgcc tgttcgcggg cggcgtgctt
     2461 tgccgccaac cgcgctcgga cgccttccat gatcgtatcg agctgggaag cgagttcgtc
     2521 gggcatgccg ttctcgtttc tggtaacgca ttccggccat ccctgaccgg ggaacgccgg
     2581 ttgaccgcgg tccttttaca gatccttcac cggctcgggg aattcgcctt cgcgggccgc
     2641 gtcgatcatt tgcctggcct cggaaggaag ctccatgccc atctcatcaa accgcttctc
     2701 gacccagcgg ccgatctggc gtggatcttt atacgaatca ccgcttcccg gctcgggaac
     2761 gccgtaatct tcgaggatct gctgctcact gcggtggtag gcaaacttcg aaactgcccg
     2821 ctccatgcgt tttccgtggc agtgaccgca taccggctct atcggagagg acacggttcg
     2881 ggcaaatacg ctcgatagtc tgccgcattc ccggcatcga tattcgtaga tcggcatcca
     2941 aaacgcctcc gcgctagccg cgggactcaa tcgcgtccgt tctaaccggc gaagtctccg
     3001 aaatcgtcgt cgtcgccgtc atagggcatg tcgcctgggt cgc
//
LOCUS       DATCPE010000024         5602 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U7219
            k141_3669, whole genome shotgun sequence.
ACCESSION   DATCPE010000024 DATCPE010000000
VERSION     DATCPE010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955033
            Sequence Read Archive: SRR1586279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 5602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 16:49:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 640
            CDSs (total)                      :: 631
            Genes (coding)                    :: 627
            CDSs (with protein)               :: 627
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5602
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3669"
                     /isolate="SMAG_U7219"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1755)
                     /gene="infB"
                     /locus_tag="VIM45_00815"
     CDS             complement(<1..1755)
                     /gene="infB"
                     /locus_tag="VIM45_00815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEY8819858.1"
                     /translation="MVTRTPQPPAGEPVDQGPIELPRVITVKELGERMSVSAIEVIKE
                     LMKNGVMATVNQTIDFDTAAVVATDLGFEPIEAEVAAQEVTPVFTSRKVTETGEGLES
                     RAPIVTIMGHVDHGKTSLLDAVRNTRVTEGEAGGITQHIGASTIIINGQRLTFLDTPG
                     HEAFTAMRARGAEVTDIAVLVVAADDGVMPQTAEAINHAKAAGVPIVVAINKIDLESA
                     NPDRVKQELLQYGITVEDYGGDVIAVPVSARTKEGLDDLLESLLAQAEILELKANPRR
                     PAIGVVLEAEMDQARGKVATVLVQTGTLRPGDVIVAGDNAWGRVRAMFDERGRKLRQA
                     GPSQPAKILGLSAIPHAGDRVTVVEDERTAREWVNQRQREQDAILANSHQAVTLDTLF
                     GEISAGKLKELNIVLKTDVQGSIEPIRNAFERMSNEEIRVKVIHSGSGSITESDVMLA
                     KAAKGIIVGFNTRPEQGARRQAEAEGVQIKVYEVIYHAVEDIEAALKGMAEPVFVEVV
                     DGHAEVRQLIKISRYGTIGGSYVTDGKVVRSSSCRIRRGHELVFEGRVENLKRFKEDV
                     REVLAGYECGITLEGWDDF"
     gene            complement(1873..2268)
                     /locus_tag="VIM45_00820"
     CDS             complement(1873..2268)
                     /locus_tag="VIM45_00820"
                     /inference="COORDINATES: protein motif:HMM:NF016204.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEY8819859.1"
                     /translation="MDASSRLKRKSSTSTNTSRRSGEAASAAPPARKQPLRPRHVPQR
                     TCVICRTTTAKRALVRLVRTSEGSVRVDPTGKQPGRGAYLCVDPDCWRAPGLQQRLGN
                     SLKTSVSNEDYRQLESFADDLAPLPPDAG"
     gene            complement(2201..3685)
                     /gene="nusA"
                     /locus_tag="VIM45_00825"
     CDS             complement(2201..3685)
                     /gene="nusA"
                     /locus_tag="VIM45_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010936687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HEY8819860.1"
                     /translation="MKNDFLLAIAQLAGEKNLSKEVVFEAVETALASAYRKDQAQSGP
                     VVVKIDQNTGTAHFYAQKTVVTDVEDDKAEMPLKEARAINPASVIGDTINIEIAAQPA
                     GRIAAQTAKQVVLQKLREAEREVVFEEYAGREGDILSGIVQRIEGSGNHKSVFIDLGK
                     TEAMLPPGEQVRTEHYQQAQRLKVYVIEVHKATKGPQVIVSRSHKNLIKRLFELEVPE
                     IFRGAVEIKSIAREAGYRSKVAVTAKQEGVDPVGACVGLRGIRIQNVVNELGGERIDV
                     VQWDPDAARFVASALSPAQVVSVSVDEEDNTASVVVPDRQLSLAIGKEGQNARLAAKL
                     TGWRIDIRSQTTAEEQRLAMSPLEPVVVAEPGPKVEEEELRPAAQATPTVVEVAAFPE
                     EIEPAVVTEPVHVPQPVAAGVAAVPARPRGLRFAEDLNVGPLVAPARPESGAKSNKKH
                     KNKKGGDRDEVELEAAHAKKGKRGRVIEVEEEEFDEYQYLTKKR"
     gene            3952..4896
                     /gene="ribF"
                     /locus_tag="VIM45_00830"
     CDS             3952..4896
                     /gene="ribF"
                     /locus_tag="VIM45_00830"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HEY8819861.1"
                     /translation="MSTRLLREELHRASPGRPSAYTIGNFDGVHRGHQFLIQLLKQRA
                     AARGLTSGVITLYPHPLTVVRPGTEIVYLTGLDERIELLQALGVDSVAPVTFTSEVSQ
                     IGAEEFMRELVDELQLQFLLIGPDFALGRGREGSPERLIEIGNHLGVEVELSPLESED
                     GHKIGSRDIRDALAAGDIDRVNYLLGRRYSLRGPVVHGAELGRTLGFPTANVAVAADR
                     ALPGYGIYNACAYLNEGVYPAAVNIGIRPTVNTGAPSVEAYLIGFTGDAYGRELRLDF
                     ARRLRGEEKFDSLEAMKSQIACDVDEAARLDCVPKAGP"
     gene            4950..5390
                     /locus_tag="VIM45_00835"
     CDS             4950..5390
                     /locus_tag="VIM45_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8819862.1"
                     /translation="MMDSDYQLDMVEVRRHLEETQIVGFFFPFIRRTLLLDTRTSAAD
                     QPMILVVPMVNSVEDRMRSLKRLRPRFPKPESMTLIPWPRFVAAIQRLGLWELIERRM
                     VALGGEAMHDRCTEAFTELLREERTQIKNAIVGEGYQTLWERTP"
     gene            complement(5387..>5602)
                     /gene="fusA"
                     /locus_tag="VIM45_00840"
     CDS             complement(5387..>5602)
                     /gene="fusA"
                     /locus_tag="VIM45_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218418.1"
                     /note="EF-G; promotes GTP-dependent translocation of the
                     ribosome during translation; many organisms have multiple
                     copies of this gene; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEY8819863.1"
                     /translation="TQGMTPAGKGHTTIEAEAPLAELQRYATDLRSITQGRGSFTMQF
                     DHYAEVPAHVAQKVIDEHKKELAKAGH"
BASE COUNT          949 a         1745 c         1730 g         1178 t
ORIGIN      
        1 gaaatcgtcc caaccctcta gggtaatgcc gcactcgtac ccggcgagca cctcgcgcac
       61 gtcttccttg aagcgcttta ggttttcgac gcggccttcg aacaccagtt catgaccgcg
      121 gcggatccgg cagcttgaag agcgcacgac tttaccgtcg gtgacgtacg aaccgccaat
      181 cgtgccataa cggctgatct tgatcagttg ccggacttct gcgtggccgt cgacgacttc
      241 gacgaagacc ggttctgcca tgcctttgag cgcggcttcg atgtcttcga ccgcgtggta
      301 gatcacctca tagaccttga tctgcacgcc ttctgcttcg gcctgccggc gggctccttg
      361 ctccggacgt gtattgaagc cgacgatgat acccttggcc gcttttgcga gcatcacgtc
      421 cgactcggta atcgagcctg agcccgagtg aatgaccttc acacggatct cttcgttgct
      481 catgcgctca aaggcgttgc gaatcggttc gatactgccc tgcacatcgg tcttgaggac
      541 gatgttcagc tctttcagct tgccggcgct aatttcgcca aacagcgtgt cgagggtgac
      601 ggcctggtga gagttggcca ggatggcgtc ttgctcgcgc tggcgctgat tcacccactc
      661 gcgcgcggtc cgctcgtcct cgaccacggt cacgcggtcg ccggcgtggg gaatcgcgct
      721 gaggccgagg atcttcgctg gctggctggg tccggcctgc cgcagcttgc gtccgcgttc
      781 gtcgaacatg gcgcgtacgc ggccccaagc gttgtcgcct gcgacgatca cgtcgccggg
      841 gcgcagagtg ccggtctgga ccaagaccgt cgcgactttg ccgcgggcct ggtccatctc
      901 ggcctcgagc acaacgccga tcgccgggcg tcgggggttg gccttcagtt cgaggatttc
      961 ggcctgcgcc agcaggctct caagcaggtc gtcgagacct tccttggtac gagcggagac
     1021 aggaaccgcg atcacgtcgc cgccgtaatc ttcgacggtg attccgtact ggagcagttc
     1081 ctgtttgacg cggtccgggt tggcgctttc gaggtcgatc ttattgattg ccacgacgat
     1141 ggggacgccc gccgccttgg cgtggtttat cgcctcggcc gtctgcggca tgacgccgtc
     1201 gtccgcagcg accaccagta cggcgatgtc cgtgacttcg gcgccacggg cgcgcatcgc
     1261 cgtgaacgct tcgtggccag gcgtgtcaag gaatgtgagg cgctggccgt tgatgataat
     1321 cgtcgaagcg ccgatgtgct gcgtaatccc gcccgcttcg ccttcggtta cgcgtgtgtt
     1381 gcgtacggcg tcgaggagac tcgtcttgcc atggtcgacg tggcccatga tcgtaacgat
     1441 cggtgcacgc gactcaagcc cttcgcccgt ctccgttacc tttcggctgg tgaagaccgg
     1501 cgttacttcc tgcgcggcga cctcggcttc aattggttca aatccgaggt ccgtggcgac
     1561 gacggcggcg gtgtcaaagt cgatcgtctg gttgacggtc gccatcaccc cgttcttcat
     1621 cagctccttg atcacttcga tggcgctgac gctcatgcgc tcgccgagct ctttgaccgt
     1681 gattacacgc ggaagctcaa tcggtccctg gtcaaccggc tcaccggccg ggggttgcgg
     1741 tgttctcgta accataattc ctcctctgcg gaggcggcag cacggacggc gggccacctc
     1801 acaagagccg ggccggtgcc cgcggctcca aggagagcgc gccgcagatg cggtgccaaa
     1861 tccggagtta ccttagccgg cgtcgggagg taggggcgcg aggtcgtccg cgaagctctc
     1921 taactgccgg tagtcttcat tcgaaactga agttttcaaa gaattgccca agcgttgttg
     1981 gagccccggc gcacgccaac aatcgggatc gacgcacagg tatgcgccac ggcccggctg
     2041 cttgcccgtt gggtctactc gtacacttcc ctccgatgtc cgcacgagcc tgacgagagc
     2101 ccgttttgcg gtggtcgtgc ggcaaatgac acacgtccgt tgcggaacat gacgggggcg
     2161 aagtggctgc ttccgggctg gtggggcagc agatgctgcc tcaccgcttc ttcgtgaggt
     2221 attggtactc gtcgaactct tcctcttcaa cctcgatgac gcgtccacgc ttgcccttct
     2281 tggcgtgtgc agcctcgagt tctacctcgt cacgatcgcc gcccttcttg ttcttatgct
     2341 tcttattaga cttggcgccg ctctccggcc gcgcgggtgc caccaacgga ccgacgttca
     2401 gatcttcggc aaagcgaagg ccgcgtggac gagcgggtac agcggcaacg ccggccgcga
     2461 ccggttgggg cacatggacg ggctccgtca cgacagccgg ctcgatctcc tcgggaaacg
     2521 cggcaacttc taccactgtc ggggtggctt gagccgctgg ccgcagttcc tcttcctcga
     2581 ccttcggtcc tggttcggcc accacgacgg gttccagcgg actcatcgcc aggcgttgct
     2641 cttccgcggt ggtctggctg cggatgtcga tccgccagcc cgtgagcttg gcggcgagtc
     2701 gagcgttctg tccctctttg ccgattgcaa gcgagagctg gcggtccggc accaccaccg
     2761 aagcggtgtt gtcttcttcg tctactgaaa cgctgacgac ctgggcaggg ctcaaagcgc
     2821 tggcgacgaa gcgcgccgcg tctggatccc actgcacgac atcgatacgc tcgccgccga
     2881 gctcgttaac gacgttctga atgcggatgc cgcgtaagcc gacgcaggcg cctacagggt
     2941 cgacgccttc ctgcttagcc gtgacggcga ctttgctgcg atagcctgcc tcgcgcgcga
     3001 tcgacttgat ctccacggcg ccgcggaaga tttcggggac ctcgagctca aatagccgct
     3061 tgatcaggtt cttgtgggag cgagaaacga tgacctgggg acccttggtc gccttatgga
     3121 cctcgatcac gtagaccttg aggcgctggg cctgctgata gtgctcggtt cgcacctgct
     3181 cgccgggcgg cagcatcgcc tccgtcttgc caaggtcgat aaaaacgctc ttgtggttgc
     3241 cgctgccctc gatccgctgc acgattccgg aaaggatgtc gccttctcga ccggcgtatt
     3301 cctcgaatac gacctctcgt tcagcctcac gcagcttttg cagcacgacc tgcttggccg
     3361 tctgggcggc gatgcgcccg gcgggctggg cagcgatttc gatgttgatc gtgtcgccga
     3421 tgaccgacgc ggggttgata gccctcgctt ccttcagcgg catctctgcc ttatcgtctt
     3481 cgacgtcagt aacgacggtc ttttgagcgt agaaatgcgc ggtgcccgtg ttctggtcga
     3541 tcttcaccac gacgggcccc gactgcgcct gatccttgcg ataggctgac gcgagcgccg
     3601 tctctactgc ttcaaagacg acctctttcg aaagattctt ctctcccgcc agctgtgcga
     3661 ttgccagcag aaagtcgttt ttcattccga gatgaacctc cgcgccacgg acacctaacg
     3721 tttaggccta acaaaaaagt gggctgtacc cacttcaacg atactaccgc cgctctgcat
     3781 tatcgtatca ccgcgaacca gaggcgcgca acggagcgga agccccgcca gcgcgcatca
     3841 cgaccgattc aggttgcccg gcggatcggc ccatgctacg atccttgaca gcgagcggtg
     3901 gtacggggag ggtaattacc ctccccgttt gtttccgcga aaggcgggcc aatgtccacc
     3961 cggctcctac gagaagagct gcatcgcgca tctcctggac gcccaagcgc ctataccatc
     4021 ggcaacttcg acggcgtcca tcgcggccac cagttcctca ttcaattgct caagcaacgc
     4081 gccgcggcca gaggcctgac cagcggggtg atcacgttgt atccccaccc gctcaccgtc
     4141 gtgcgtccgg gtaccgaaat tgtctacttg accgggctgg acgagcggat cgagctgttg
     4201 caagcgctgg gtgtcgatag cgtggcgccc gtgactttta cgtccgaggt ctcgcagata
     4261 ggcgccgaag agtttatgcg ggagcttgtg gacgagctgc agttacagtt cttgttgatc
     4321 ggccccgact tcgcgctggg acgcggacgc gagggttcgc cggagcgcct gatcgagatc
     4381 ggcaaccatt tgggcgtcga ggtggaactc tcgccgctgg aaagtgaaga cggccataag
     4441 atcggctcgc gcgacatccg cgacgcactg gcagccggag acatcgatcg tgtcaactat
     4501 cttctggggc ggcgttactc gctgcgcgga ccggtggtac acggcgccga gcttggacgc
     4561 actctcggtt ttcctaccgc caacgttgcc gttgcagcgg acagggcgct tccaggctac
     4621 ggcatttaca acgcctgcgc ttatttgaat gagggtgtat atcccgcggc ggtgaacatc
     4681 gggattcgac ccaccgtgaa caccggcgca ccgagcgtgg aagcctacct gatcggcttc
     4741 accggtgacg catacggccg cgaactgcga ctcgacttcg cgcggcggtt acggggtgaa
     4801 gagaagttcg atagtctgga ggcgatgaag tcccagatcg cctgcgacgt ggacgaagcg
     4861 gcgaggctcg actgcgttcc aaaggccgga ccgtaaagag cagtcagggc gacgtgcacc
     4921 agtacacgca aacatagtaa ggaaagccga tgatggattc cgattaccag ctcgatatgg
     4981 tcgaagttcg ccggcacctg gaagaaaccc agattgtcgg ctttttcttc ccgttcatac
     5041 ggcgcacctt gctgcttgat acacggacca gtgccgccga tcagccgatg atcctcgtcg
     5101 tgccgatggt caactcggtg gaggaccgca tgcggagcct gaagcggttg cgaccgcgat
     5161 ttccgaagcc cgagtcgatg acgctcatcc cctggccgag gtttgttgcc gcaattcagc
     5221 gcctgggtct ctgggaattg attgagcggc gcatggtagc gctcggcggc gaggcaatgc
     5281 acgaccgctg caccgaagcg tttaccgagc ttctccgcga agagcggacc cagatcaaga
     5341 acgcgatcgt cggcgaaggc taccagacgt tatgggagcg caccccctaa tgtccggctt
     5401 tggcaagctc cttcttgtgc tcgtcgatta ctttctgggc gacgtgggcg ggcacttcgg
     5461 cgtaatggtc gaactgcatc gtgaacgagc cgcggccctg ggtgattgag cgaaggtcgg
     5521 tggcgtaccg ttgcagttcg gccaaggggg cctcggcttc gatggtcgtg tggcccttgc
     5581 ccgccggcgt catgccctgg gt
//
LOCUS       DATCPE010000025         7143 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U7219
            k141_295545, whole genome shotgun sequence.
ACCESSION   DATCPE010000025 DATCPE010000000
VERSION     DATCPE010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955033
            Sequence Read Archive: SRR1586279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 7143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 16:49:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 640
            CDSs (total)                      :: 631
            Genes (coding)                    :: 627
            CDSs (with protein)               :: 627
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7143
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295545"
                     /isolate="SMAG_U7219"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1209
                     /locus_tag="VIM45_00845"
     CDS             <1..1209
                     /locus_tag="VIM45_00845"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEY8819864.1"
                     /translation="GSRTARLVLRTLDDLQELIAAVQLGVTMASLALGALAEPTIATV
                     IEPWFVFVPHAWRPVSAHGIALVFTFAFITALDIVVAELVPKTIALQNSERVAFVVIR
                     PVRAFIWLFRPVIAVLNRAGGAIVRLTGLRPPGEGDAAHSPEELKMLVAASTRAGVLN
                     TDEQEMLFRVFEFSRLTARQVMVPRTEMLALDIEMTREQVYEILGNTHHTRYPVYRGT
                     IDDIAGILYVRDLLNRFTSHEQIEFDLRQLVREPYFVPDSVPIDDLLAQLRREQVQIA
                     VVLDEFGGTAGLVTLEDVLERIVGEVRDEFEESGPEIQMLAEGDAVIDGLTLVGDVNE
                     RFALALDAEDYDTLGGLVWGELGRLPQVGDILEIEGCRLAVEAMDGRRVAAVRLSRSG
                     SGGDAVESAN"
     gene            1230..2744
                     /locus_tag="VIM45_00850"
     CDS             1230..2744
                     /locus_tag="VIM45_00850"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl--CoA ligase"
                     /protein_id="HEY8819865.1"
                     /translation="MAEGSQTLIDLLEAPQQSRTAVIAPERDLKLSYADLRRFVSQAA
                     EGLRALGVKLGDHMALVAPNGPEFIFGYLGALEAGTACQPINPQLKPDEVDFAVEDSS
                     AVLTLVVAGSEGTLHGTRVPEGRRAVLRFDGGSVAIEGRPIGPPQKGGGPSGDSDALI
                     LYTSGTTSRPKAVPLTHKNLVTSARNVADSYQLTADDVTMLVMPLFHVHGLVAATFAT
                     LLRGGTIIAPPRFSASGFWDPVRQYRATWYTAVPTIHTILLNSVTDSDAKSAGQFRFV
                     RSCSSALAAPTQAQYEETFGVPVLQAYGMTEAAHQIATNPLPPGDRREDSVGLPYGVD
                     VAILDDAGDALPSGAEGEVALRGPNVTRGYLHNPNANRDAFTNGWFRTGDSGYIDADG
                     YIYLSGRLKELINRAGEKISPHEVDAALLSHPSVYEAVAIAVPHPIYGEEVEAVVALK
                     PGHSADAAELISFCRDRIADFKVPKAIRFVAEVPRSATGKIQRRRLLEMLPPVS"
     gene            complement(2774..3376)
                     /gene="cobU"
                     /locus_tag="VIM45_00855"
     CDS             complement(2774..3376)
                     /gene="cobU"
                     /locus_tag="VIM45_00855"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012188608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HEY8819866.1"
                     /translation="MGSLILVTGGARSGKTRFALRLADRLPAPRIYVATAEAGDEEMA
                     SRIARHRAERAPNWHTLELPLGLDTRIAVSSNDGDTVLIDCLSVWLSNELLAGHDWSD
                     AGDASSAVLSASVSREAEERLLSMVERFAQFGAERAGVTIVVTNEVGSGIVPAFPLGR
                     LYRDILGRANQLVAARAAAVYLVVAGIGVDLRRLEAGLAD"
     gene            complement(3383..4885)
                     /locus_tag="VIM45_00860"
     CDS             complement(3383..4885)
                     /locus_tag="VIM45_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006522520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HEY8819867.1"
                     /translation="MPGKVIMLQGTASSVGKSLLCTALCRILHQDGLRVAPFKAQNMS
                     NNSFVTPDGLEFGRAQAAQAEAAGIEPSVEMNPVLLKPEADHRSQVVVMGRQRGVVGG
                     ADFISIKRDLWPVVTDALDRLRQQYEVIVIEGAGSPAEINLKAGDIVNMRVARYAQAP
                     VLLAGDIDRGGVFAHLVGTLQLLEPEERALVRALVINKFRGTASLLQPGVDWLTEYTG
                     LPVAGVIGWLADVGVADEDAVALEQRSRIGVAKTFDIAVVQFPRIANFDDLDALASEP
                     DVDLRYITKGDEVADADLLILPGTKSTMADLGWLRATGIAAEVVRHAAAGGPIIGVCG
                     GYQMLGERLLDPEGVESPIAEVQGLGLLPVVTIFEAIKATHRAIARVSAAHGLLNGLE
                     GAELAGYEIHTGRTAGARPALWVLSRSGESCDAGDGAIDASGAIFGTYLHGIFDNDAF
                     RRSVLGWLAQRRGIERDSSGVSWGREQAYDRLANHVRSALDMPLIYRLLD"
     gene            complement(4891..5526)
                     /locus_tag="VIM45_00865"
     CDS             complement(4891..5526)
                     /locus_tag="VIM45_00865"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEY8819868.1"
                     /translation="MTTRLLLTRHGETESNAGHRFSGHSDVALTSLGHAQARALGRRL
                     RREPIDVAYSSDLARARETAMIALRGRSVKVTTHSGLRELCFGDWEGKTFQEVREGWP
                     DSYRQLLVVGDGFCAPGGEPLEITRERVVGAMSEIVARHESLNVLIVAHGGTLQILLA
                     HVLGMPPSSMFRLATGNCGLSIIEFHGERPIVTLVNDCVHATRVDKPTARG"
     gene            complement(5572..6669)
                     /locus_tag="VIM45_00870"
     CDS             complement(5572..6669)
                     /locus_tag="VIM45_00870"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEY8819869.1"
                     /translation="MRVTGAVHGGVRPGELEALGLNLADVLDFSVNVSPIGPPPGVRD
                     AIMALEPGAYPDPDCTVLTNALSSLLDVPGKQILIGNGSTQLIHLVTRVFVHQGQRPV
                     VFAPTFGEFAHAVEILGANAYPWQAQQQRAFRWNLKNKPDVLRRVAPPLVYLCNPNNP
                     TGVYLGRDEVRGLAQALMSGPLLLDEAYRGFLAEPWASLDMVRTGRVIVLRSMTKDYA
                     LAGLRLGYLVAHEDVVAAVRRLQPAWSVSSFAQAAGVAALRDEEYLPRMRRIVTEGKS
                     ALVTGLESIGMSVHEGAVNFILCDVGDATDFRRRLLLRGIAVRDCSSFGLPQFIRIGV
                     RTPVECKRLVEEMSALLKELEGAQAEIGILG"
     gene            complement(6660..>7143)
                     /locus_tag="VIM45_00875"
     CDS             complement(6660..>7143)
                     /locus_tag="VIM45_00875"
                     /inference="COORDINATES: protein motif:HMM:NF015164.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_function: GO:0048472 - threonine-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CobD/CbiB family cobalamin biosynthesis protein"
                     /protein_id="HEY8819870.1"
                     /translation="SVVAPLLAYCVAGLPGAYWYRCVNTLDSMIGYRGRFEWIGKTAA
                     RLDDLANFIPARISAGLLIVSAPFIGGSSTQGLRLLLEDRRRTESPNAGWTMAAAAGS
                     LGVRLEKQGQYVLNRNGREPGPADIATARLLVAVASTASVLLAAVAILRRPLRSNVCG
                     "
BASE COUNT         1362 a         2328 c         2169 g         1284 t
ORIGIN      
        1 ggtagccgca cggcccggct ggtgcttcgg acgctggacg atcttcaaga gctaattgca
       61 gcggtccagc tcggcgtgac gatggcaagt cttgcactcg gcgccttggc ggagccaacg
      121 atagcgaccg tcatcgagcc atggttcgtc ttcgtgcctc atgcctggcg cccggtcagc
      181 gcccacggaa tcgcgctcgt ctttacgttc gcatttatta ctgcgctcga tatcgtcgtc
      241 gcggagctgg tgccaaagac gatcgcgctc cagaacagtg agcgcgtcgc attcgtcgtc
      301 atccggcccg tccgcgcctt catctggctc ttccgtccag ttatcgccgt cctcaatcgg
      361 gcgggcggcg ccattgtccg gctgacgggg ctacgcccgc ccggcgaggg cgacgcggcg
      421 cattcacccg aggagctgaa gatgctcgtt gccgccagca ctcgtgccgg agtgctgaac
      481 accgacgaac aggaaatgct ctttcgcgtc tttgagtttt cacgccttac tgcccgccag
      541 gtgatggtgc cacgcacgga gatgctggcg ctcgacatcg agatgactcg cgagcaggtt
      601 tacgagattc tgggaaacac ccaccatacg aggtatccgg tataccgagg gacgatcgac
      661 gatatcgccg gcattttata cgtgcgcgac ctgctgaacc gattcacatc acacgaacag
      721 attgagtttg accttcgtca actggtccgc gaaccgtatt tcgtgccgga tagcgtgccg
      781 atcgacgacc tgctcgccca attgcggcgc gagcaggtgc agatcgcggt ggtgctcgac
      841 gagttcggtg gtaccgccgg cctggtgacg ctggaggacg tgttggaacg gatcgtcggc
      901 gaagttcgcg acgagtttga agagtccggc ccggagattc agatgttagc cgagggcgac
      961 gcggttatag acgggctaac cctcgtgggc gacgtgaacg aacggtttgc gctcgccctt
     1021 gacgcggagg actatgacac gctcggaggg ttagtgtggg gcgagcttgg gcggctaccc
     1081 caggttgggg acatacttga aattgagggc tgccggctgg cagtcgaggc gatggacgga
     1141 cggcgagtcg cggcggtcag gctcagccgc agtgggtccg gcggcgatgc cgtcgaaagc
     1201 gccaattaga cgaaggggtt gaaacaacca tggcagaagg ttcacagacg ctcatcgatc
     1261 tgctggaagc cccgcaacag tcacggacgg ccgtgatcgc tccggagcgt gacctgaaac
     1321 taagttacgc agatttgcgg cggttcgtct cgcaggcggc tgagggactc cgggccctgg
     1381 gcgtgaagct gggcgaccac atggcgttag ttgcgccaaa cgggccagag ttcatttttg
     1441 gctacctggg ggcgcttgag gcaggcaccg cttgccagcc catcaaccct caactcaagc
     1501 cggacgaggt cgacttcgcg gttgaggact cctccgccgt tctcacgctc gtcgtggccg
     1561 gcagcgaagg aacgctccac ggtacgcggg tgccggaggg gcgccgagcc gttctgcgtt
     1621 tcgacggtgg ttcggtcgcg atcgaaggcc ggccgatcgg cccgcctcag aagggaggcg
     1681 gaccgtcggg cgacagcgat gcgctgattc tctacacatc gggaacgacg agccgtccca
     1741 aagcggtacc gttgacgcac aagaacctgg tcacatcggc caggaacgtc gccgactcat
     1801 accaattgac cgccgacgac gtcacaatgc tcgtcatgcc gttgtttcac gttcacggcc
     1861 tcgtggcggc aacatttgcg acgctgctcc gcggcgggac gatcatcgcc ccaccccgct
     1921 tcagcgcatc gggcttctgg gacccggtgc gccagtaccg tgcgacgtgg tatacagcgg
     1981 taccgaccat ccacaccatc ctcctcaaca gcgtcacgga ttcggacgca aagagcgccg
     2041 ggcaattccg cttcgtgcgt tcgtgctcgt cggcgctggc cgcgcccacg caggcgcagt
     2101 atgaggagac gttcggcgtc cccgtactgc aggcctacgg catgacagag gcggcacatc
     2161 agattgcgac aaacccgctt ccgcccggcg accgtcgcga agattcggtt ggcctgccgt
     2221 acggggtgga cgtagcgatc ctcgacgatg ccggcgacgc gcttccgtct ggcgcagaag
     2281 gcgaagttgc gctgcgcggg cctaacgtta cgcgcgggta cctgcacaac ccgaacgcca
     2341 acagagacgc gttcacaaac ggctggttcc gcaccggcga cagcggttat atcgacgccg
     2401 acggctacat ctacctttcc ggccggctca aggagctcat caatcgtgct ggcgaaaaga
     2461 tctcccccca cgaggtcgac gccgcgttgc tcagccaccc gtcggtgtat gaggcggtcg
     2521 cgatcgccgt tccacacccg atctacggtg aagaggtcga ggcggtggtc gcattgaagc
     2581 ccggccacag cgccgacgcg gcggagctca taagcttctg cagggaccgg atagccgatt
     2641 tcaaagtgcc gaaagcgatc cgtttcgtgg ccgaagttcc tcgttctgcc accggcaaga
     2701 tccagagacg gcggttgttg gagatgctgc cgccggtttc ctagtggact cgaaggtcgg
     2761 catcagcgcg taatcagtcc gcgagccctg cttccagacg gcgcaggtcc acgccgattc
     2821 cggcgacgac gagataaacg gccgcggcgc gcgccgcgac aagctggttg gcgcgcccta
     2881 ggatgtcgcg gtacagcctg ccaagcggga aggcgggcac gatccctgag ccgacctcgt
     2941 tggtaaccac gatcgtcacg ccggctcttt cggcgccaaa ctgggcgaac cgttcgacca
     3001 tcgacagtag ccgctcctcg gcttcgcggc tcacggaggc actcagcacg gctgacgaag
     3061 cgtcgccggc atcggaccaa tcgtgaccgg caagcagttc gttgctcagc catacactga
     3121 ggcaatcgat caagacggtg tctccgtcat tgctgctcac ggcaatgcgt gtgtccaacc
     3181 caagcggcag ctcgagtgtg tgccagttcg gtgcccgctc ggcgcggtga cgggcgatcc
     3241 gagatgccat ctcttcatca ccggcttccg cggtcgcaac gtagatccgg ggcgcaggca
     3301 agcggtcggc gaggcgaagc gcaaatcgcg tcttgccgct gcgggctccg ccggtaacta
     3361 gtatcaggct gcccatttat aactagtcca gcaggcgata gatcaggggc atgtcaagcg
     3421 ctgaccgcac atgatttgca aggcggtcat aggcttgctc gcggccccag ctcacaccag
     3481 atgaatcgcg ctcgatgcct cgacgctgcg ccagccagcc cagcacagag cgcctgaaag
     3541 cgtcgttgtc gaagatcccg tggaggtagg tgccgaatat cgcaccgctg gcgtcgatgg
     3601 cgccgtcacc cgcatcacaa ctctctcccg aacgtgatag gacccacaat gccggccgcg
     3661 ctccagcagt ccgacccgtg tgaatctcgt aaccggccag ctcggcgccc tccagtccgt
     3721 ttaacagccc atgcgcggcc gaaacccgcg cgatggcccg gtgcgtcgcc ttgatggctt
     3781 caaatatcgt gacaaccggc aagaggccca gtccttgaac ctcggcgatc ggcgactcca
     3841 cgccctcggg gtccagtaga cgttctccca gcatctggta gcccccgcag acgccgataa
     3901 tcggtccgcc ggcggcggca tgcctcacga cctcggctgc gataccggtg gcccgcagcc
     3961 acccgaggtc cgccatcgtc gacttggttc ccggaaggat cagcaggtcg gcatccgcta
     4021 cttcgtcgcc tttcgttatg taacgaagat cgacgtcggg ttccgatgcc agtgcgtcca
     4081 ggtcgtcgaa gttcgcgatg cgtggaaatt gcaccacagc gatatcgaac gtcttagcga
     4141 cgccaatcct cgatcgctgc tcgagcgcga ccgcgtcctc gtcggctacg ccgacatctg
     4201 ccagccagcc aataacacct gctaccggta gacccgtgta ttccgttagc cagtcgaccc
     4261 ccggctgcag gaggctggcg gtaccgcgga acttgttgat taccagcgcc cgtacgagcg
     4321 cgcgctcctc gggctccaac agttgcaacg tgccgaccag atgtgcgaaa acaccgccgc
     4381 gatcgatgtc gccggcaagc agtaccggcg cctgagcgta ccgtgccacc cgcatattga
     4441 cgatgtcgcc ggctttgaga ttgatctcgg ctgggctacc tgcaccttcg atcacgatga
     4501 cctcgtactg ctgccgcaat cggtcgagag cgtctgtcac gacaggccag aggtcgcgct
     4561 taatgctgat gaagtctgca ccgccgacga ccccacgctg ccggcccatg accaccacct
     4621 gggagcggtg gtcggcctcc ggcttgagca gcaccgggtt catctctaca gacggctcga
     4681 tccccgcggc ttcagcctgg gcggcctggg cccgtccgaa ctcgagaccg tccggcgtca
     4741 cgaacgaatt gttggacatg ttctgcgcct tgaacggcgc aacgcgaagt ccatcctggt
     4801 gcaagatacg gcagagcgcc gtacacaaca ggctcttccc tacggacgac gccgtgccct
     4861 gcagcatgat cacttttccg ggcatagcta ttatccacga gccgtcggct tgtcgacccg
     4921 cgtcgcatga acgcagtcgt tgaccaacgt gacaatcggt cgctcgccgt gaaactcgat
     4981 gatactgaga ccgcagttcc cggtcgcgag ccggaacatg ctgctgggcg gcataccgag
     5041 cacatgcgct agcaggatct gcagcgtgcc tccatgcgct acgatcagca cgttcaggct
     5101 ctcatgccgc gcgacaatct cgctcatggc gccgaccacc cgttcacggg tgatttcgag
     5161 cggctcacca cccggagcgc aaaagccgtc gccgaccacc agaagctggc ggtacgagtc
     5221 cggccacccc tcgcgcactt cctggaacgt cttcccttcc cagtctccga aacatagctc
     5281 gcgcaggcct gaatgtgtgg tcaccttcac tgagcggccg cgcaaagcga tcatcgccgt
     5341 ctcccgtgct ctggcaaggt cgctgctgta cgccacatcg atgggctcgc gacgcaaccg
     5401 acggcctagt gctcgcgcct gagcgtgccc cagcgaggtc agggccacgt ccgagtgccc
     5461 ggaaaatcgg tgacccgcgt ttgattctgt ttcgccatgc cgcgtcagca gtagcctggt
     5521 tgtcaaaagt tctttctccg cagcgagccg ccgctgctcg tttccaaact ttcagccgag
     5581 aatgccgatc tctgcctgtg ctccctcaag ctctttcagc aaagctgaca tctcctctac
     5641 caggcgcttg cactcgaccg gcgttctaac gccgatccgg atgaattgag gcaagccaaa
     5701 cgaagaacaa tcccgaacgg cgatgccccg cagcagcagg cggcgacgaa aatcggtcgc
     5761 atcgccgaca tcacacagaa tgaaattcac cgccccctca tgcacagaca tgccgattga
     5821 ctccaggccg gtcaccagcg cactcttgcc ctccgttaca atgcgtctca tgcgtgggag
     5881 gtattcctca tcgcgtagcg ctgctactcc cgcggcctgt gcaaagctgc taacgctcca
     5941 ggccggttgg agcctgcgaa cggccgcgac aacgtcttcg tgggctacca ggtagcccag
     6001 cctgaggccg gcaagggcat aatccttggt cattgagcgg agcacgatca cgcggccggt
     6061 ccggaccatg tccagagacg cccacggttc cgccaagaat ccgcgatacg cctcgtccaa
     6121 cagcaacgga cccgacatca gcgcctgtgc gaggccgcgg acttcgtcgc gcccgaggta
     6181 gacacctgtg gggttgtttg ggttgcaaag gtataccaag ggcggcgcaa cccgccgcaa
     6241 tacgtccggc ttgttcttca agttccagcg gaacgcacgt tgctgctgcg cctgccacgg
     6301 gtaggcgttc gctcccagaa tctctacagc gtgcgcaaac tcgccgaacg taggcgcgaa
     6361 aacgaccggg cgctggccct ggtggacgaa cacccgcgtg accagatgaa tcaattgcgt
     6421 agacccgtta ccgatcagga tctgtttgcc aggtacgtcc aggagtgagc tcagagcgtt
     6481 cgtcaaaacg gtgcaatcag gatcgggata tgcgcccggc tcgagcgcca tgatcgcgtc
     6541 acgcacgccg ggtggagggc cgatagggct gacgttgacg ctgaagtcca gcacgtcggc
     6601 caggtttaga ccgagagctt ccagctcacc cggcctcacg ccaccgtgca cagcgccggt
     6661 tacccgcata cgttgctccg tagcggtcgc cgaaggatag cgacggccgc cagaagtacc
     6721 gatgccgttg aggcgacagc caccagcagc ctggcggtcg caatatctgc aggcccgggc
     6781 tcgcgtccat ttcggttcaa cacgtactga ccctgctttt cgagccgtac tccgagcgat
     6841 ccggccgcgg ccgccatcgt ccatcccgcg tttggactct ccgtgcggcg gcggtcctcc
     6901 agtaggagtc gcaggccctg agtcgatgaa cccccaatga acggcgcaga cacaatgagc
     6961 agaccagcac tgatcctcgc cgggatgaaa ttcgccaggt cgtcgaggcg cgcggcggtt
     7021 ttaccaatcc attcaaagcg gccccggtac ccgatcatgc tatcaagcgt attcacgcag
     7081 cgataccaat aagccccggg aaggccagcg acacaataag cgagcaacgg cgcgacaacg
     7141 ctg
//
LOCUS       DATCPE010000026         3574 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U7219
            k141_458285, whole genome shotgun sequence.
ACCESSION   DATCPE010000026 DATCPE010000000
VERSION     DATCPE010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955033
            Sequence Read Archive: SRR1586279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 16:49:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 640
            CDSs (total)                      :: 631
            Genes (coding)                    :: 627
            CDSs (with protein)               :: 627
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3574
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_458285"
                     /isolate="SMAG_U7219"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /locus_tag="VIM45_00880"
     CDS             <1..410
                     /locus_tag="VIM45_00880"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEY8819871.1"
                     /translation="CEMELSYTTWVRAALNADNPDVGGLSTSPVAIPIEAANGHDVHE
                     LLGIMATERLGTLALIEGLGLDDFDRPGTHPMFGTLTVLQWLRSFYRHDRMHFDQIAG
                     RDPDYRPRFTGAEPNQRTARLAKVQHERQERRG"
     gene            468..1469
                     /locus_tag="VIM45_00885"
     CDS             468..1469
                     /locus_tag="VIM45_00885"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HEY8819872.1"
                     /translation="MTNRPHVFVTRHLPGDALEMLRERANVDLWEDALPPPRQVLLDR
                     SRHAEGLISLLTDKIDTELLEACPRLSVVANYAVGYDNIDVPACTQAGVFATNTPGVL
                     TNTTADFAFALLMAAGRRVVEGDRFTRENRWKTWGPELLLGTDVYGGTLGLVGVGQIG
                     SAVARRARGFDMHVLYHDAVPRPDLEESLGLQRTDLETLLRESDFVSLHVPLLPETHH
                     LISDRQLELMKRTAVLVNTSRGPVVDGAALYRALKAGRPAAAGLDVTETEPISPEDPL
                     LTLPNVIIAPHIASGSFVTRSRMAQMAVDNVLAGMEGRVPPDCLNPQATERHRGRGD"
     gene            1594..2259
                     /locus_tag="VIM45_00890"
     CDS             1594..2259
                     /locus_tag="VIM45_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HEY8819873.1"
                     /translation="MARSNAAAGKSWLAKELWDLDAIQFGDFTLGRTLNTPVYVNLRR
                     LVSNPAALQRVGRVIKEESETLLTMLHQHMDPFSRCAGVPFGGLHLATAFSLTARVPM
                     IYLHPQKDGSGSVIEGVYIPGQTVLVIDDLISGGTSIIETAAQLREAGLHVKDAIALV
                     DRRQGGKERLRQHGIRLIPLLDLEMLLNWGMANGRIEDEAQYRASIDFLHSRGTEAKD
                     EAV"
     gene            complement(2317..2670)
                     /locus_tag="VIM45_00895"
     CDS             complement(2317..2670)
                     /locus_tag="VIM45_00895"
                     /inference="COORDINATES: protein motif:HMM:NF037170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY8819874.1"
                     /translation="MVEDWARPVVHWEVQARDAQRLRDFYAQMFNWVIGEGPIMNIPA
                     GVGGPQPGPGGHIRQSEKPGVSLYIQVLNLRASLEKAVQLGGSVHVQPVDSPGRATLA
                     GIADPEGNRIMLVQQ"
     gene            complement(2755..>3574)
                     /locus_tag="VIM45_00900"
     CDS             complement(2755..>3574)
                     /locus_tag="VIM45_00900"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEY8819875.1"
                     /translation="ALTDRGTQSAKDYTFRAPPDSVQSLVGAGITVVDVANNHVLDFG
                     TQGLLDTISTVARNGIFHIGAGSDGESARTPAIVMVNGIRIAWLAYVNVPDDSISGFV
                     ARSLEGGPGRPGVAWGTPDVIARDVTAARQRSDVVIVALHSGFEYTSEPNSVQKDLAH
                     AAIDAGAALVLGAHPHVLQGMEFYHGGLIAYSLGNFLFDLDASDIAHYDLPSVLTVVL
                     RVNLDIRGVTGIEVYPAIINRNDFRPEPVSGDAARPVFDRIYKLTDGLNGGNRP"
BASE COUNT          660 a         1123 c         1113 g          678 t
ORIGIN      
        1 tctgcgagat ggagctttcc tacacgacct gggtgcgcgc cgcgctaaac gcggataatc
       61 cggacgtggg agggctctcg acgtcacccg ttgcaatccc gatcgaagca gcaaacggcc
      121 acgacgtcca tgaactgctc ggcatcatgg ccaccgagcg cctcggcacg cttgccctta
      181 tcgaggggct ggggctggac gacttcgacc ggcccggcac gcacccgatg tttggcacgc
      241 tcaccgttct tcagtggctg cgatcgtttt acaggcatga ccgcatgcat ttcgaccaga
      301 tagcgggcag ggatcccgat tatcggccca ggttcaccgg agcggagcca aatcaacgga
      361 ccgcgcgcct ggccaaagtt caacatgagc gccaggagag acgcgggtga cccgcccgcc
      421 cggtcgccgc ggaccgtacc gctatccagc attgcggagg tgagcgcttg accaacaggc
      481 cacacgtctt cgtcacccgt cacctccccg gcgacgccct cgaaatgctc cgcgaacggg
      541 cgaacgtgga cctctgggag gacgcgctgc cgccgccccg ccaggtattg ctggatcggt
      601 cgcgccacgc cgagggactt atctcgctac tcacggacaa gatcgacacc gaactgctcg
      661 aggcctgtcc gcgactctcg gtcgtggcta actacgcggt cgggtatgac aacatcgacg
      721 tgccggcgtg cacccaggcg ggcgtcttcg ctactaatac tcccggcgtc ctgaccaaca
      781 ccaccgccga tttcgcgttc gctctattga tggcggccgg gcggcgcgtg gtagaggggg
      841 accggttcac gcgcgagaat cggtggaaga cgtggggtcc ggagcttttg ttgggtaccg
      901 acgtttacgg cggcacgctc gggcttgtcg gcgtggggca gatcggctcg gcggttgcgc
      961 ggcgggcgcg cggcttcgac atgcacgtgc tgtatcacga tgcagtgccg cgccccgatc
     1021 tcgaagaatc gctcgggttg cagcgcaccg acctggagac gttgctgcgc gagtccgatt
     1081 tcgtcagcct ccacgtgccc ttgcttcccg aaacgcacca cctcatcagc gatcgccagc
     1141 ttgaattgat gaagcgcaca gccgtgctgg tgaacacatc tcgtggaccg gtggtggacg
     1201 gcgcggcgct ctaccgtgcg ctgaaggcgg gacgccccgc cgccgcggga ctcgacgtca
     1261 cggaaacgga accgatctct cccgaagacc cgctacttac gttgcccaac gtgatcattg
     1321 cgccgcacat tgctagcggc tcgtttgtca cgcgttcacg aatggctcag atggcggtgg
     1381 ataacgttct ggccggtatg gagggccggg tgccgcccga ctgcctcaac cctcaggcca
     1441 ctgagcgcca tcgcggtcgc ggggattgac aaaatcacgt gcaactttct agcctttgtt
     1501 cagttaatgc cgatcaaagc gttcagatga tattctcatg tcgacaagcc gcgtcaggcg
     1561 accggctttt ttcatatcta cggcggtggt gacatggcgc gatcgaacgc ggccgctggg
     1621 aaatcctggc tggcaaaaga gctgtgggat ctggatgcga tccaattcgg cgactttacc
     1681 ctgggacgga cgctgaacac gccggtatat gtcaatctgc gacggctggt gagcaacccg
     1741 gccgccctgc agcgggtagg acgggtcatt aaagaagagt ccgagaccct gttgacgatg
     1801 ctgcaccagc atatggatcc attcagccgt tgcgccgggg taccgttcgg cgggctgcac
     1861 ctggccaccg cgttctcgct gaccgcgcgg gttcccatga tctacctgca tccgcagaag
     1921 gacggctcgg gcagcgtcat cgaaggcgtc tacatccccg gccagacagt gctggtgatc
     1981 gacgacctga tcagcggtgg cacgtcaatc attgagaccg ccgcacaact gcgcgaagcc
     2041 ggtctacacg taaaggacgc gatcgcgctg gtcgatcgtc gacagggcgg aaaagagcgc
     2101 ctacggcaac acggcatccg gctgattccg ctgctcgacc tggagatgct gctgaactgg
     2161 ggcatggcta acggccgcat cgaggacgaa gcccagtacc gcgccagtat tgacttcctc
     2221 cactcgcggg gcacggaggc caaggacgag gcggtttaga cgcctctcac aagagccggg
     2281 ttcaccgccg tgcggtacaa ccgaagctcc ggcgctttat tgctgcacca gcatgattcg
     2341 attgccttcg ggatcggcga ttccggcgag cgtcgctctg cccggcgagt cgaccggctg
     2401 cacatgcacg ctgcctccta actgcacggc tttctcaagt gacgcccgta agttgagcac
     2461 ctggatgtac agactgaccc cgggtttttc gctttgccgg atatgtccgc cgggtcccgg
     2521 ttgcggcccg ccgacaccgg ccgggatatt catgatcggc ccttcgccta tcacccagtt
     2581 gaacatctgc gcgtagaaat cgcgcagtct ttgcgcgtcg cgcgcctgga cctcccagtg
     2641 gactaccggt cgagcccagt cttccaccat cgcgatctct cctgtttgaa atgccgtgcc
     2701 aagtctagcc cgacctggtg tccatgatct agcggttggg tcgtcggact ggatttacgg
     2761 tcggtttccg ccgttgagcc catccgtaag tttgtagatc cgatcgaaca ccggccgggc
     2821 ggcatcgccc gaaaccggct cgggacgaaa gtcgttgcgg ttgatgatcg cgggatacac
     2881 ctcaatgccg gttacgccgc gaatgtccag gttcacccgc aagacaacgg tgagcactga
     2941 gggcagatcg taatgcgcga tgtcggatgc gtccaggtcg aacaggaaat tgcccagact
     3001 gtaggcgatg aggccgccgt ggtagaattc catcccttgc aggacgtgag ggtgcgcgcc
     3061 aagaacgagc gccgcgcccg catcgattgc ggcgtgtgcg aggtctttct gcacgctgtt
     3121 tggctcagaa gtatattcga agcccgagtg caaggcgacg atcacgacgt cgctacgttg
     3181 acgggcggcg gtcacgtcgc gggcaatcac atccggtgta ccccatgcga ctcccggtct
     3241 gccggggcct ccttccaggc tgcgggccac gaatcctgag atactgtcgt ccggcacgtt
     3301 gacatacgcc agccaggcga tccggattcc attcaccatg acgatagcgg gagtgcgtgc
     3361 agattcgccg tcggaaccgg caccgatgtg aaatatgccg ttccgcgcga ccgtgctaat
     3421 cgtgtccagg agtccctgag taccgaagtc cagcacgtgg ttgttcgcaa cgtcgaccac
     3481 ggtgatgcct gcgcctacga ggctctgcac cgaatcaggc ggcgctcgaa aggtgtagtc
     3541 cttcgccgac tgtgtgcctc gatcggtgag cgcg
//
LOCUS       DATCPE010000027         4661 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U7219
            k141_166871, whole genome shotgun sequence.
ACCESSION   DATCPE010000027 DATCPE010000000
VERSION     DATCPE010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955033
            Sequence Read Archive: SRR1586279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 4661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 16:49:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 640
            CDSs (total)                      :: 631
            Genes (coding)                    :: 627
            CDSs (with protein)               :: 627
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4661
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166871"
                     /isolate="SMAG_U7219"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..692)
                     /locus_tag="VIM45_00905"
     CDS             complement(<1..692)
                     /locus_tag="VIM45_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY8819876.1"
                     /translation="MTTTQDPNTAKGANATDWIDREGKYYFQAARRMPIVIERGEGTA
                     VFDTDGKRYLDFVAGIATNSLGHRHPAVVDAIKKQADTLIHISNVYFSEPQVQLAQLL
                     VEQSALDRVWFCNSGAEANEAAIKLARKWGGMHKDGAFEIITTTNSFHGRTLAAVTAT
                     GTERYKVPFGPLPPGFIVVPFDDVEALRAAVTPKTCAIMLEPVQGEGGVNLPSENYLQ
                     AVRKLCDDQNILL"
     gene            complement(929..3832)
                     /locus_tag="VIM45_00910"
     CDS             complement(929..3832)
                     /locus_tag="VIM45_00910"
                     /inference="COORDINATES: protein motif:HMM:NF015644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HEY8819877.1"
                     /translation="MSFGEPPREDRGPLGPPPIQFRVGREQLPHINAARWLVIAVAVF
                     VLFIAASIGKGIYADYLWFDSVGLSSVYGKEILTKVILFFASALIFLLFIGFNLWLAR
                     RLAPEGYEESFIAEVEPATLRRIVTIGLVAGSLFLAVIFGSVAAGQWDTVLRFANRVD
                     FGVNDPAFNHDVGFYVFALPALRALQGWLVGAVIVTLLAVVGVYAFTLSLQNFELRLS
                     RGNKGHVGALLIGLLLLFCFGYVLSIFELAIGHNGVVQGATYTDIHARVPGYFILFAF
                     SGIAMAAIVWSVLRKSLIPAGLGGMAWVIVLIVVLGLYPGSVQRFTVDPNELAKEQPY
                     IERNIKMTRQAFGLDSIQEQPFDAETNISADAISANQETINNIRLWDPRFIINAYKQQ
                     QEIRPLYVFDDVDVDRYQIDNAYTEVTLSPRELTLSGLPVNARSWVNIHLQYTHGYGA
                     VMNPVNKVDQDGLPAYNLGNIPPAGAPPITEPRVYYGSETNEYVIIGGKQSEFDYESD
                     TGQAETRYSGKGGIPIGSFFRRLVYAWEFSDTNLLISGQITGTSRLLYRRNIVDRINH
                     IAPFLRLDQDPYLVVAGGKLYWMQDAYTTSSNYPYSEVSSSGFNYIRNSVKVVVDAYT
                     GEVTFYLADANDPIARTYQRMFPSLLKPISQMPDELRSHVRYPEDLFTIQADVFRTYH
                     IQDSRAFYNKEDLWATPKEGASGNVKDVVPYYVIMKLPGESNEEFVLIRPFTAANKPN
                     AVAWMGARSDGENYGKLAVYRFPSGRQIPGPAQVDSSIDGQPDISQRFSLLNVQGSHV
                     QRGNLLLIPIGNSYLYVEPVYLVADQNPKPAVIAVIVYNGDKVYMEPTFQQALKVAIG
                     QAQPTFAVGATSAGNQAAASQAQRAATPAPATPAPAPTPSSTVAPTVRAGPSPSATDV
                     AGLAREALDADAQAQQALRQGDFAGYGQQQARLKAALDRLSQLVGQPAASPTP"
     gene            complement(3851..>4661)
                     /gene="argB"
                     /locus_tag="VIM45_00915"
     CDS             complement(3851..>4661)
                     /gene="argB"
                     /locus_tag="VIM45_00915"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEY8819878.1"
                     /translation="SRDFVWEPRRVPEFNQTVVVKLGGSTLGAHDTTLSDIAALHRRG
                     VPIVVVHGGGAEITRWLDIHKVESHFVDGLRVTHGPTLDVVVAVIAGLINKQLVAQLA
                     GLGVRALGLCGADGGLLRAEIDQPALGFVGAVREVDVALLQMLLNAGIVPVIAPIAIH
                     IGKGSGQLLNVNADTVAGEIAAVLNHCRLVFLTDVAGVQDGNGDLVHRLTSEDTNRLR
                     GSGVLKGGMIPKIEACFRAAAAGSRPVIVDGRQEHALTKAIGTEPVGTLVG"
BASE COUNT          925 a         1381 c         1407 g          948 t
ORIGIN      
        1 aggagaatgt tttgatcgtc gcaaagtttg cgcacggcct gcagatagtt ttctgagggc
       61 aggtttaccc cgccttcgcc ttgcacgggc tccagcatga tggcgcacgt ttttggtgtc
      121 accgctgccc gaagcgcctc gacgtcgtcg aatggaacga caatgaagcc tggcggcagc
      181 ggtccaaacg gaaccttgta gcgctcggtt ccggtagccg tcacggcggc aagggtgcgc
      241 ccatggaacg agttggtggt cgtaatgatc tcaaatgcgc cgtccttgtg catgccgccc
      301 cacttacggg ccagcttaat tgcagcctcg ttcgcttctg cgccgctgtt gcagaaccag
      361 acgcgatcga gtgccgactg ttcaaccagg agttgagcga gttgtacctg tggctcgctg
      421 aagtacacgt ttgagatgtg gatgagagtg tcggcctgct tcttgatcgc gtcgactacc
      481 gcgggatggc gatgtccgag tgaattcgtg gcgatgccgg ccacgaagtc caggtaccgc
      541 ttgccgtcgg tatcgaagac ggccgtacct tctccgcgct cgataacgat tggcatgcgt
      601 cgtgcggcct ggaagtaata tttgccctcg cggtcgatcc agtcggtcgc gttagcgcct
      661 tttgccgtat tagggtcttg tgtcgtggtc acctgattct cctacagctt cggtatcgct
      721 gggttcccgc tgttgcctga ctaacgccag accccgctca ctgcattgca gctgcagcca
      781 agtcgcgatg gccatcccct cttgccttaa acgctagagt ctcgtctact atctcccctt
      841 ccttggtggg gaaggggccg gaagttaggt ctggcgggcc ggggttgagg tctacgacct
      901 ccgactgccg actgccgact gccctcggct acggcgttgg cgacgctgct ggctggccta
      961 cgagctggct cagccggtcg agcgccgcct tgagtcgtgc ctgttgctgg ccatagccag
     1021 cgaagtctcc ttgacgcagt gcctgttgag cctgcgcgtc ggcgtccaga gcctcccgcg
     1081 ctagccctgc cacatcggtt gccgatggcg acggcccggc ccgtactgtc ggcgccacgg
     1141 tcgaagatgg cgttggtgca ggtgcaggcg tggctggagc gggcgtcgcg gcgcgctgcg
     1201 cctggctggc ggcggcttgg ttgcctgcgc tggttgcgcc aacggcaaat gtcggttgcg
     1261 cctgtccgat tgcgaccttc agagcctgct gaaatgtcgg ctccatatac accttgtcgc
     1321 cgttgtatac gatcacggcg atcacggccg gcttggggtt ctggtcggct accaggtata
     1381 ccggctcgac atacagatac gaattgccga tcggaatcag cagcagatta ccgcgctgaa
     1441 cgtgcgaccc ctgaacgttc aacagtgaga accgttgtga aatgtccggc tggccgtcga
     1501 tgctcgagtc gacctgggcc gggccgggga tctgccgtcc cgatgggaac cggtacacgg
     1561 ccagttttcc gtaattttct ccatcggagc gggcgcccat ccaggcgacc gcgtttggtt
     1621 tgttggccgc ggtgaatggc cgaatgagga caaactcctc gttcgattcg cccggcagtt
     1681 tcatgatcac gtagtaggga accacgtcct tgacgttacc gctggcgcct tccttcggcg
     1741 ttgcccacag gtcctctttg ttgtaaaacg cccgcgaatc ctggatgtgg taggtgcgga
     1801 agacgtccgc ctgaatcgta aagaggtctt ccggatagcg gacgtgcgac cgcagttcgt
     1861 ccggcatttg cgaaattggc ttcaacaggc tcggaaacat cctctgatag gtcctggcga
     1921 tcgggtcgtt cgcatccgcc agatagaagg tcacctcgcc ggtgtacgcg tcgacgacga
     1981 ccttcacact gttacgaatg tagttgaagc cggagctgct gacttccgaa tagggatagt
     2041 tggacgacgt cgtgtatgcg tcctgcatcc agtacagctt cccgccggca accaccaggt
     2101 agggatcctg gtcgagtcgc aggaacggtg cgatatggtt gatgcgatcg acgatattgc
     2161 gccggtaaag caggcggcta gtgcccgtaa tttgaccgct gatcaggagg ttggtgtcac
     2221 tgaactccca tgcgtagacg agccgccgga aaaacgaccc gatcggaatc ccgccctttc
     2281 ccgagtaacg ggtttcagcc tgacccgtgt ccgattcgta atcgaactcc gattgcttac
     2341 cgccaatgat gacgtactca ttcgtttcgc tgccgtagta gacacggggc tcggtgattg
     2401 gcggggcgcc tgccggcggg atattgccca ggttataagc gggaagaccg tcctggtcga
     2461 ccttgttgac gggattcatg acagcgccgt aaccgtgggt gtattggaga tggatgttca
     2521 cccagctccg agcgtttacc ggcagaccag agagcgtcaa ttcacgcggc gacagcgtta
     2581 cttcggtata ggcgttgtcg atctgatagc gatccacgtc gacgtcgtcg aacacataca
     2641 gcggacgaat ctcctgctgc tgcttgtacg cgttgataat gaaccgaggg tcccacagcc
     2701 gaatattgtt gatcgtctct tgattggcgc tgattgcgtc cgccgaaatg ttcgtttcag
     2761 catcgaatgg ttgttcctgg atcgagtcga gaccaaaggc ttggcgcgtc atcttgatgt
     2821 tgcgctcgat gtacggctgt tccttggcca gctcattggg atcgaccgta aagcgctgga
     2881 cggaacccgg ataaagaccg agtacgacga tcagcacaat cacccaggcc ataccgccaa
     2941 gtcccgcggg aattaagctc ttgcgtaaca ccgaccagac aatggctgcc atagctatgc
     3001 cgctaaacgc gaacaggatg aaatagccgg gaacgcgggc gtggatatca gtatacgtag
     3061 cgccctgcac cacgccgtta tggccgatag ccagttcgaa aatgctcagc acgtagccaa
     3121 agcagaagag caacagcaac ccgatgagca gcgcaccgac gtgcccttta ttgccgcgac
     3181 tgagacggag ctcgaagttc tgcaagctca gcgtaaaagc gtagacgccg accacggcca
     3241 gcaaggtgac gatcactgcg ccgaccaacc aaccctgcag cgcgcgcagt gcgggcaacg
     3301 cgaacacgta gaagccaacg tcgtgattga acgcaggatc gttcacgccg aagtcgacgc
     3361 gattggcgaa acgcagcacc gtgtcccact gtcccgccgc taccgagcca aagatcaccg
     3421 cgagaaacag tgagccggcg accaagccga tggtcacaat gcggcgtagc gtggcgggct
     3481 ctacttccgc gatgaagctt tcttcgtagc cctccggcgc cagccgtcgc gcgagccaca
     3541 ggttgaaacc gatgaagaga agaaaaatca aggcgcttgc gaagaagagg atgaccttgg
     3601 tcaggatctc cttgccatac accgacgaga ggccgacgct gtcgaaccag aggtaatcgg
     3661 cgtagatccc cttgccaatc gacgcagcaa tgaacagcac gaatacagca acggcgatta
     3721 cgagccagcg cgccgcgttg atatgcggaa gctgctcacg cccaacacgg aattggatag
     3781 ggggcggacc aagcggtccg cggtcttctc taggtggctc gccgaacgac actgtgcctc
     3841 cgcctctctg tcagccgacg agtgtcccca ccggctcggt tccaatggcc ttggtcaatg
     3901 cgtgttcctg tcgcccgtcg acaatcaccg gccgcgaacc ggcggccgcg gcgcgaaagc
     3961 aagcctcgat cttcgggatc ataccgcctt tcagaacacc cgatccacgt agacggtttg
     4021 tatcctcgga tgtcaaccga tgaacgaggt caccgttgcc gtcttgtacg ccggcaacgt
     4081 cggtcagaaa gacaagccgg cagtgattga gcaccgccgc gatctcacca gctaccgtat
     4141 cagcgttcac gttgagcagc tgtcccgagc cttttccgat gtgaatggca atcggagcga
     4201 tgaccggaac gatgccggcg ttcaagagca tttggagcaa ggcgacgtcc acttctcgta
     4261 cggcgccgac gaatccaagt gccggctggt caatctctgc tcgaagcaac ccgccatccg
     4321 cgccgcagag ccccaacgcg cgcacgccca ggcctgcaag ctgggcaacc agttgcttgt
     4381 tgatcaggcc cgcgatgacg gcgacgacga cgtcgagtgt tggaccatgc gtcacccgga
     4441 ggccgtcgac aaaatgcgac tcgactttgt gaatgtcgag ccagcgcgta atctcggcgc
     4501 cgccgccgtg gacgacgaca atagggacac cgcggcggtg caacgcggcg atgtcgctca
     4561 acgtcgtgtc gtgggcgccg agcgtgctgc cgccgagctt tactacaacg gtttggttga
     4621 attcgggcac cctgcgaggc tcccagacga aatcgcgact c
//
LOCUS       DATCPE010000028        13127 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U7219
            k141_231505, whole genome shotgun sequence.
ACCESSION   DATCPE010000028 DATCPE010000000
VERSION     DATCPE010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955033
            Sequence Read Archive: SRR1586279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 13127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 16:49:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 640
            CDSs (total)                      :: 631
            Genes (coding)                    :: 627
            CDSs (with protein)               :: 627
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13127
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231505"
                     /isolate="SMAG_U7219"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..692)
                     /gene="ftsZ"
                     /locus_tag="VIM45_00920"
     CDS             complement(<1..692)
                     /gene="ftsZ"
                     /locus_tag="VIM45_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014435065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEY8819879.1"
                     /translation="MKQAARAEVEGLPPIKVVGVGGGGCNAVNRMIQEHIFGVQFIAV
                     NTDAQQLMHSDAPTKIRIGDKLTRGLGVGGDPTIGLRAAEESREELREAIRGADMVFV
                     TAGMGGGTGTGAAPVLAEVAKQEGALTIGVVTKPFSFEGSRRLKQAEAGIQALREKVD
                     TLIVIPNDRLLSMCDKRVTVQEAFKAADDVLRQGIRGISEIITRPGEVNLDFNDVRKV
                     MSDAGPALMAIG"
     gene            complement(832..2076)
                     /gene="ftsA"
                     /locus_tag="VIM45_00925"
     CDS             complement(832..2076)
                     /gene="ftsA"
                     /locus_tag="VIM45_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEY8819880.1"
                     /translation="MSRRGTFAAIDVGTTKVCTIVGDADRDGGPRVLGVGVVPSSGLT
                     KGMVENIHDATEAIRVSVEKAERSSGQRVTSAHVGIAGAHISSLNNRGIAAIADRDHA
                     ISNDDVDRALDGARGLSIPTNREVLHVIPRYYVVDGQDNVSDPVGMYGQRLDVETHII
                     TGAVSAMRNLTKCVEGAGIEVDALVLEPLASAEAVLLDEERRQGVVLVDIGGGTTDVG
                     IFVDGSVFHTAVIPVGGYHLTHDLVVGLRVPFDSAEAAKAQYGNALPSNVRADEMIDL
                     DAFGGDGRMHVHRQRLCEILQARTEEILEMVLTEVHRAGYDEMVSAGLVLTGGSSNLT
                     GIADLARQVLNMPVRIGMPSGVYGLVETISNPAYATSVGLLHWAMRDGGQYTHPAQVG
                     PRMAMPEWMKGFRKLIRGFLPE"
     gene            complement(2112..2885)
                     /locus_tag="VIM45_00930"
     CDS             complement(2112..2885)
                     /locus_tag="VIM45_00930"
                     /inference="COORDINATES: protein motif:HMM:NF015739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HEY8819881.1"
                     /translation="MRRRPGRGQRAAYLPPRHHNQHRHPFTRGTFTAVVLLLLATGCG
                     YGGWRLYNSGWLIIQKVQVEGASTVSEDDIREAAALQGQQILNVNTGAAAARVNALAW
                     VQTASVERHFLNSITIRIVERTPIGVWRVGADSYLVDGEGVVIEAATDFGDLPIVDAT
                     QSGFDVRPGDHVEGDSLRTAQQVLTLASKISGQTIAAFTYDKDNGLTAVTSTGTRVLL
                     GDGQNLDYKLAVWQAIASKVNPDDIHELDLRYGNRPFYR"
     gene            complement(2899..3843)
                     /gene="murB"
                     /locus_tag="VIM45_00935"
     CDS             complement(2899..3843)
                     /gene="murB"
                     /locus_tag="VIM45_00935"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEY8819882.1"
                     /translation="MTKIAAGRLDRLAKELNPLGGLQRDEPLARHTTFGIGGPADLYF
                     KATSSDILTAAARAARRAGVPHFVFGSGSNILVGDAGCRGLVIENDAKQIEGPTPDGH
                     GQGCVRGESGASFATTARRLCRQGFWGLEWAVGIPGTLGGAVVYNAGAYGGCLADVLR
                     EIDLLEPDDTRRVMAASELSLEYRGSVFTRGLLRDRTIVSVTFDVRQGDPAEIMAHVA
                     KLDEKRIATQPRGRNAGSIFKNPPEHPAWWLIDQVGLRGARIGDAEISAKHANFFSNV
                     GQAKACEIKQLMDLASGLVRERFGIELHAEVALVGEGF"
     gene            complement(3840..5240)
                     /gene="murC"
                     /locus_tag="VIM45_00940"
     CDS             complement(3840..5240)
                     /gene="murC"
                     /locus_tag="VIM45_00940"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEY8819883.1"
                     /translation="MVERRIELSRLKRVHLVGIGGMHMSAIASILLSRGVTVTGSDIA
                     PSPFTERLEREGATVYPRHDAANLGDPDLVVATVAAKETNPEILAARERKIPVVIRAE
                     MVAALMEGRIAVCVAGTHGKTTTSSLLAYALREAGRDPTYLLGGDSVDLGRNAAPGNG
                     PEIVVEADEYAEAFLHYSPDIALVTNVEVDHLDYYRTEERLFGAFAAFMARVRPDGTL
                     IVCADSPRLVELVERSTNGSQPPVRARIESYALDRVADWTARIVEGGAHQVFSVEREG
                     QPFGVFTTPLAGRHSVSNCLGAIATLHTLGIGLPDMQRTIAGFHGARRRFEHVGEVRG
                     VTIMDDYAHHPTEIAVNIAAARDRFPGRRLVIVFQPHTFSRTSYLFDGFRSCFDGADK
                     VLLLQTYAARENAGAGMDAHQLADAMSPRPDVAEDLNEAVSWLGANTRRGDVVFTMGA
                     GTVDAVGKAFTGSAPV"
     gene            complement(5240..6331)
                     /gene="murG"
                     /locus_tag="VIM45_00945"
     CDS             complement(5240..6331)
                     /gene="murG"
                     /locus_tag="VIM45_00945"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEY8819884.1"
                     /translation="MRVLLTGGGTGGHVYPALSVAEVLPERAGGEQIEFLFVGTTRGD
                     VERLVTRAGMAFTSVDAAPVRGRGPIALIGAVWRISAGFVQSWRILGKFRPGVVLATG
                     GYASVPVSVAAWLRRIPLVVYLPDVYPGLAVRLLVRLARRVATTTDGAKEYLPPSKTV
                     VTGYPVRAEFWSQNRNAARIRFGFDEKTPVLLVTGATQGSMALNTAVIDALPRLLCDC
                     AILHQTGLSGIGAARAAAARLPFESRERYQPVAYLDDMPAAMAAADLAVMRSGASSLA
                     EPPAAGLPAILVPGSFAGAHQRHNASYMASRGAAVILAEKRLCELGDLAVALLTDKDR
                     LANMKAAATALARPDAALRIADVVLEAVA"
     gene            complement(6328..7566)
                     /gene="ftsW"
                     /locus_tag="VIM45_00950"
     CDS             complement(6328..7566)
                     /gene="ftsW"
                     /locus_tag="VIM45_00950"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HEY8819885.1"
                     /translation="MAAVARGRRNYAGRPDYWILALAIILTGIGLLAVYSASFAIGYS
                     DFHNVNYFITKQSISAVAGIALLLIFQRVPYDYLRRYSPLIMLFSLVSLLIVLLPHFG
                     VRSNGANRWIAIGPLPPLEPSEFAKLAMVIYISAWLAAKGDHVKQFSLGVVPFVMMVG
                     LVGGLILLEPDFGTAIVITLTTMTMFFVAGAALPHVLTLLLSGVAGAGLMISVEGYQS
                     ARLTSFLSPEKDPAGTGFHILQLLIALGSGGIHGLGLGASRQKFFYVPGAHTDGIFAI
                     IGEEMGLIGGLVVIFLFAGLVYRGFRAGVKSPDKFAFLLAVGITCWIGYQAVINIGGI
                     TRSIPMSGIPLPLVSYGGSALIATLAAIGVLLNVSRHLGEAGVMERERSGGAVPGGRR
                     VRSEPRNRSRKRRLIGRGRK"
     gene            complement(7554..8957)
                     /gene="murD"
                     /locus_tag="VIM45_00955"
     CDS             complement(7554..8957)
                     /gene="murD"
                     /locus_tag="VIM45_00955"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEY8819886.1"
                     /translation="MNDEWLHDRRVAVLGLGIEGRDLSRFLAARGARLIVFDTRSRNA
                     VAAGAAELEALGAEVRLGPIPVDAADGCDAIYVSQSVLLHREPLVLRARATGKPVRSR
                     QTEFFERWPGPIAAITGSSGKTTTTSLVQAGFAAAGVPHIVGGNIGGSLLGQLDAAAP
                     NCWAVLEVSHTQLQLFERGPDIACVTNVTPNHLDQFSWDEYVDLKRNLVRHQSRRDVA
                     VLNATNPDAARLAFDTQAKKVWFNADVPGEDSCFVSDEQLVLRTADATTAFVTLGEVP
                     LRGRHNVENVLAAAAVTASARIPPTIFGDAVRSFKPVPHRLEYVANVDGAAWYNDSIA
                     TSPERTLAGLRSFEEPLVLLLGGREKNLPLDELAEEVHKRVRAVICFGEAAGLLAGAI
                     ERQEANGAPRAQVQRVKTLDEAVSMARSWSQPGDVVLLSPACTSFDAYPNFERRGEEF
                     RRLVLNEARKVGAPSQL"
     gene            complement(8960..9931)
                     /gene="mraY"
                     /locus_tag="VIM45_00960"
     CDS             complement(8960..9931)
                     /gene="mraY"
                     /locus_tag="VIM45_00960"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEY8819887.1"
                     /translation="MSRAFVTGVIAFLLAAGAGGPIIGQLRTRKVGKAISEFAPPSHQ
                     SKAGTPTMGGIIVFFTVMIVTVPSNIVGRYSILLPFGTIAATGLIGFFDDLATLQGRV
                     RDALSWRLKFGLIAIIGLIVGVVLYKALGIHRVAVPWLGHRNIGWGIIPLSVLIIVGT
                     TSAVAVTDGADMLAGGTTAFAFAAYGAIAAFQGQPFLGAFCYTVVGAILGFLWFNAYP
                     AMVIMGDTGALALGSSLAVVALMTEQWLILPVIGVVFVAEAFSDVLQIGYFKLTHGKR
                     ILRKAPLHHHFEMMGWSEVQVVMRFWLVGIAGAMIGVALALKVSGPL"
     gene            complement(9955..11697)
                     /locus_tag="VIM45_00965"
     CDS             complement(9955..11697)
                     /locus_tag="VIM45_00965"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEY8819888.1"
                     /translation="MAVILVLAAAGIVCRLVYLQVLQHDVYRAQASEEHVNTATEPAH
                     RGSLLDTNGHPLATSVSTYDVIINPAVWSDPRVARDGAEALAPLLNRTPDDVLALAGN
                     GGNGQVTIASGVEYQVGQQIIALSINGTLKGVQASPDSLRLYPDGDIASQVLGFLGRD
                     GTGLSGLELDLNQQLAGQPGTEQFERDSLGNPIAFGSRKIVPPQPGGDVVLTIDRNIQ
                     KMAEDELHAGVDKTHATGGNVIVMDPITGAILAMATEPTFKLSQLDLNNTNQAPLYKN
                     GAVANMFEPGSVFKLVTMSAGIDTGKVNANTTYLDTGTEVVNERIFHNFDLSTNGTTT
                     MTQVLVRSLNLGTLWLSTKVLGPDIFYKYVRLFGFGEPSGSGFSGEANGILRTNAEPD
                     WTRADLASNSFGQGISVTALQMVTAVATIVNGGNLMQPYIVKEIHANGTVRTTAPVVR
                     RRAISAETATTLRDMMRQVLEANALANIAGYTAGGKSGTAYVPTLVTKDSAGDAYANE
                     VTIPSYIGFAPLNNPRVLIYVKLDNLKSADFGGTLTAPMFSHLAGEILDYMNVPTERP
                     LPPGYERSVPATGR"
     gene            complement(11833..12270)
                     /locus_tag="VIM45_00970"
     CDS             complement(11833..12270)
                     /locus_tag="VIM45_00970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8819889.1"
                     /translation="MTVAHGPMATPHNRPILGSFGAAVSLLIAVALIVAATLLPVMQD
                     SDATTAGYAIRNNQQELADLNAQTYNIQAEIARLSSMERIRQQATQLGMVPAPRQTES
                     VVINAAAPIETLLPRRFVPQPPVVAKPPQRGLLANFLHFLSLR"
     gene            complement(12303..>13127)
                     /gene="rsmH"
                     /locus_tag="VIM45_00975"
     CDS             complement(12303..>13127)
                     /gene="rsmH"
                     /locus_tag="VIM45_00975"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:TIGR00006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEY8819890.1"
                     /translation="GGHAERILDESSPNGSLLGLDRDHEALSLARVRLQRFGARAALV
                     HANYAEAVAVAADAGMTPLTGLLLDLGVSSLQLDRPERGFSFRAEGPLDMRFDPTTGE
                     SANDLVNGAGEEELADVIWRYGEERASRRIARAIVRSRPIRTTTQLARIIERVVARPG
                     QRVSPATRTFQALRIAVNRELENLASILEDSRSLLESGGRLAVITFHSLEDRIVKQFI
                     QRESRDCICPPGLPECRCGHTASFRPINRSPITPSEDETRRNPRSRSAKLRAAVRV"
BASE COUNT         2467 a         4131 c         4005 g         2524 t
ORIGIN      
        1 tgaccgatcg ccatcagcgc cgggccggcg tcgctcatta ccttgcgaac gtcgttgaaa
       61 tcgagattga cctcgcccgg ccgcgtaatg atctctgaaa taccgcggat accctggcgc
      121 aagacatcgt ccgcggcctt gaacgcctcc tgcaccgtca cgcgtttatc acacatggaa
      181 aggaggcggt catttggaat cacgatcaac gtatcgactt tttcgcgcag cgcttgaatt
      241 cctgcttcgg cctgcttcaa gcggcgcgaa ccctcgaagc tgaatggttt agtaacgacg
      301 ccgattgtca gcgcgccttc ctgcttcgcc acttcggcga ggacgggcgc cgcccctgtt
      361 ccggtgccgc cgcccatgcc cgcggtgacg aacaccatgt cggcgccgcg gatcgcttcg
      421 cgcaactctt cgcggctttc ctcagccgcg cgcaacccga tcgtcgggtc tcccccgaca
      481 cccagcccgc gcgtcagttt gtcgccaatg cggatctttg tcggcgcatc ggaatgcatc
      541 agttgctgcg cgtcggtgtt caccgcgatg aactgaacac caaaaatgtg ttcctggatc
      601 atgcgattca cagcgttgca gccaccaccg ccgacgccca ctactttgat aggaggaagc
      661 ccttcgactt cggctctggc tgcctgcttc atcgattcct ccgtcctctt ttcgcgcggc
      721 gcgggccgtt gaggcatgaa tgtcatattt acaaaactcc tgcggttcag cagacttgcg
      781 ccgacagtgg ccgcgaccgg cagacagcag cggtacggaa aataccgacc gctactctgg
      841 cagaaagccc cgtatcagtt tacggaaccc cttcatccac tccggcatgg ccattcgagg
      901 accgacctgg gccggatggg tatactgccc gccgtcgcgc atggcccagt gtagcagacc
      961 aacactggtg gcgtatgcag gatttgaaat agtttctaca agcccataca cgcctgatgg
     1021 catacctatc cgaacgggca tattgaggac ttgcctcgcg aggtcggcga ttcccgtcag
     1081 gtttgaactg ccgccggtga gcacgaggcc ggcggaaacc atctcgtcgt aaccggcgcg
     1141 gtgcacttcg gttaggacca tctccaggat ctcttcagtg cgcgcctgga ggatttcgca
     1201 tagtctttgc cgatggacgt gcatccggcc atctccgccg aacgcatcga gatcgatcat
     1261 ttcgtcggcg cgaacattgc tcggtaacgc attgccgtac tgtgctttcg ccgcttcggc
     1321 cgagtcgaaa ggtacgcgga gtcccacaac gaggtcgtgc gttaggtgat atccgccgac
     1381 aggaatgacg gccgtatgga acaccgagcc atcgacgaag ataccgacat cggtcgtccc
     1441 gccgccaata tctacgagta cgacgccctg ccggcgctct tcgtccagca gcacggcctc
     1501 agcgctggcg agcggttcca aaacgagtgc gtccacttcg atgccggccc cctcgacgca
     1561 ctttgtcagg ttgcgcatcg ccgataccgc accggtgatg atatgggtct cgacgtcgag
     1621 acgctgtccg tacatgccga ccgggtcgga tacgttgtcc tgtccatcta ccacgtagta
     1681 gcgaggaatg acgtgcagaa cctcgcggtt ggtgggaatg ctgagcccgc gggcaccgtc
     1741 cagggcgcgg tccacgtcgt cgttgctgat cgcgtgatcc cgatcagcga tcgcggcaat
     1801 cccccgattg ttgagtgaac tgatgtgggc gcccgcgatg ccaacgtgtg cggacgtgac
     1861 tcgctggccg ctcgaacgct cggccttttc cacggaaacg cgaattgcct cggtcgcgtc
     1921 gtgtatattc tcgaccatgc ctttggtgag gccggaagaa ggcacgactc ccacgcccaa
     1981 gacacgcggt cctccatcac ggtcggcgtc gcctacaatc gtgcaaacct ttgtggttcc
     2041 aacgtcgatc gcggcaaacg ttcctctgcg tgacacggtc cgtctcctgc gtttagtggt
     2101 accgagtccg tttatcggta gaatggtcgg ttgccatacc gaaggtctag ctcatgaatg
     2161 tcgtcgggat tgactttact cgcaatcgcc tgccacacgg caagcttgta gtccagattt
     2221 tgcccgtcgc ccagaagaac tcgtgtgccg gtgctggtca cagcggtcaa gccgttatct
     2281 ttgtcgtaag taaatgccgc gattgtttga ccggagattt tagacgcgag cgtaagtacc
     2341 tgttgggcgg tgcgcaacga gtcgccttcc acgtggtcac ctggtcgcac gtcgaaacct
     2401 gactgcgtcg cgtcgacaat cggcagatcg ccgaagtcag ttgcagcctc gatgacgacg
     2461 ccttcgccat cgacgaggta gctgtcggcc cctacccgcc aaacgccgat gggtgtgcgt
     2521 tcgacgatcc ggatggttat cgagtttaga aagtggcgct ctactgaagc agtttggacc
     2581 catgccagcg cattcacacg cgccgctgcc gcgcctgtat tcacgttgag aatttgttgg
     2641 ccttgaaggg cagccgcttc gcgtatgtcg tcttcgctga ccgtcgacgc gccttcgact
     2701 tgaacctttt ggatgatgag ccagccggaa ttgtataacc gccatccacc gtaaccgcag
     2761 ccggttgcca gcagtaacag cactaccgca gtgaacgtcc cacgagtgaa tgggtggcgg
     2821 tgctggttgt gatgcctcgg aggtagatat gccgctcgct gaccgcgacc gggtcgtcgt
     2881 cgcattcgct ccgccatatc agaaaccctc gccgaccagc gcgacttcgg catgcaactc
     2941 gatgccgaac cgctctcgta cgagcccgct cgcaaggtcc atcagttgtt tgatttcgca
     3001 cgctttcgcc tgcccgacgt tggaaaagaa gttggcatgc ttggcggata tctccgcgtc
     3061 gccgatgcgg gcgccgcgca gcccaacctg gtcaatcagc caccaggccg gatgctccgg
     3121 agggttcttg aagatcgatc cggcgttccg gccgcgaggc tgtgtggcga tccgcttttc
     3181 gtcgagtttg gcaacgtggg ccatgatctc ggcaggatct ccctgtcgca cgtcgaacgt
     3241 taccgatacg atcgtgcgat cgcgtagcag tccgcgtgtg aacacgctgc cacgatactc
     3301 gagcgaaagc tccgaagccg ccatgactcg ccgggtgtca tccggttcga gcaggtcgat
     3361 ttcacgcagc acatccgcca ggcagccacc ataggcgccg gcattgtaga ccacggcgcc
     3421 tcccaaggtc ccgggaatgc caacagccca ttcgaggccc cagaaccctt gccggcacag
     3481 acggcgggct gtggtggcaa agctggcgcc gctctcaccc cggacgcatc cttgaccgtg
     3541 cccgtcgggt gtcggccctt cgatctgttt agcgtcgttc tcgatcacga ggccgcgaca
     3601 tcccgcgtct cccaccagga tgttgctgcc ggagccgaac acgaagtgcg gcacgccggc
     3661 gcggcgcgcc gcccgggcag cagcagtcag tatgtcgctg ctggtcgctt tgaaatagag
     3721 gtcggccgga ccgccgatgc cgaaggtcgt gtgccgcgcc aacggctcgt cgcgctgcag
     3781 accgccaaga gggttcagct cttttgccag gcggtcgagc cggccggcgg ctatcttcgt
     3841 cataccggcg ccgaccctgt gaatgctttt ccgaccgcgt caacggtgcc ggcccccatt
     3901 gtgaatacga cgtccccacg tcgggtgttg gctcccagcc agctaactgc ttcattgaga
     3961 tcctccgcga catccggccg tggcgacatg gcgtccgcaa gctggtgcgc atccatgccg
     4021 gcacctgcgt tctcacgcgc ggcatacgtc tgcagcagca gaaccttatc cgcgccgtcg
     4081 aagcaggaac ggaaaccgtc gaataggtaa ctggtccggc tgaacgtgtg cggttgaaag
     4141 acgatcacca accggcgtcc aggaaagcgg tcacgcgcgg ctgcaatatt cacggcgatc
     4201 tcggtgggat ggtgggcgta gtcgtccatg atcgtaacgc ctcttacctc gccgacgtgc
     4261 tcgaaccgcc ggcgagcacc atggaacccc gcgatggtcc gttgcatgtc gggtaggcca
     4321 atgcccaacg tgtgcagcgt ggcaatggca cccaggcagt tactgacact gtggcggccc
     4381 gccagcggcg tcgtgaaaac tccaaagggc tgtccttctc gctccaccga aaacacctgg
     4441 tgtgcaccgc cctccacgat gcgcgccgtc cagtcagcca cccgatccag cgcatacgac
     4501 tctatccgtg ctcgaacagg cggctgcgac ccgttcgtcg agcgctcgac tagttcgacc
     4561 agtcgcggac tgtcggcgca cacgatcagc gtcccatcgg gtcgcacccg tgccataaag
     4621 gcggcaaacg caccgaaaag tcgttcttcg gtgcggtagt agtcgagatg gtccacctcg
     4681 acgttggtga cgagtgcgat gtctggcgag tagtgcagga acgcctccgc atattcgtcg
     4741 gcttcaacca cgatctcagg tccgttaccg ggggcagcgt tgcggcccag atccaccgag
     4801 tcgccgccga gcaggtaggt tgggtcacga ccggcctcgc gaagcgcgta ggcaagcaag
     4861 ctcgatgtcg tggtcttgcc atgggttccg gcgacgcaca cggcgatccg tccctccatg
     4921 agtgccgcga ccatttccgc gcggattacc accgggatct ttcgttcccg cgccgcgagg
     4981 atttcggggt ttgtctcctt tgcagcgacc gtcgcgacca cgaggtcggg atcgcccaga
     5041 ttggccgcgt cgtgcctcgg gtagacggtg gcgccttcgc gctccaaccg ctcggtaaat
     5101 ggcgatggag cgatatcaga tccggttacg gtcacaccgc gcgacaacaa aatgctggcg
     5161 atcgccgaca tgtgcatgcc gccgatcccg accaggtgta cgcgcttgag gcgtgagagc
     5221 tcgatgcgcc gttccaccat taggcgaccg cctcgagcac gacgtcggcg atccgaaggg
     5281 cagcgtcagg tcgggcaagc gccgtcgcag ctgctttcat gttcgcgagc cgatctttgt
     5341 cggtcagaag ggcgacggcg aggtccccaa gctcgcacaa tcgtttctcc gcgagaatta
     5401 ccgcggcgcc gcgtgaggcc atgtacgaag cgttatgccg ctggtgggcg ccggcaaagc
     5461 tgccgggaac gagaatagcc ggcaggcccg cggcaggcgg ctcagccagg gacgaggcgc
     5521 cagaacgcat cacggccagg tcagcggcgg ccatcgcagc cggcatgtcg tccagatagg
     5581 cgacgggctg atacctctcg cgggattcaa agggtagccg cgcagcggcg gcacgagccg
     5641 cgcctattcc ggagagccct gtctgatgca gaatagcgca atcacagagc agcctcggca
     5701 aagcgtcaat cacggccgtg ttcaacgcca tcgaaccttg tgttgccccc gtgacgagca
     5761 acacaggcgt cttctcgtcg aagccgaaac gtatacgcgc ggcgttgcgg ttttgactcc
     5821 agaattcggc gcgaaccgga tacccggtca cgacggtctt gctcggcggg agatactctt
     5881 ttgctccgtc cgtcgtcgtc gccacgcgcc ttgcgagacg aaccagcaaa cgcaccgcca
     5941 acccgggata gacgtccgga agatacacga ccagcgggat gcgacgcagc cacgccgcga
     6001 ccgaaaccgg cacgctcgcg tagccgcctg tcgcgaggac gacacccgga cggaactttc
     6061 cgaggatgcg ccacgactgc acaaacccgg cgctgattcg ccagacggcc ccgatcagcg
     6121 cgattggtcc tcgaccgcga accggtgcag catctacgga cgtaaaggcc attcccgccc
     6181 tcgtaaccag ccgctcgacg tcgccgcggg tcgtgccaac aaacaggaac tcgatctgtt
     6241 cgcctccggc gcgttcgggg agcacttcgg cgacgctgag cgccgggtat acgtgtcctc
     6301 ccgttccgcc accggtgagc aacactctca ttttcgtccc cggccgatca gccggcgctt
     6361 cctggatcta tttcgcggtt cggaccttac acggcggccg ccgggcaccg cgcctcccga
     6421 ccgttcgcgt tccataacgc cggcctcacc taaatggcgc gatacgttca acagcacgcc
     6481 gatggcggcc aacgttgcaa tcagcgcaga accaccgtaa ctcaccagcg gcagcgggat
     6541 tccggacatc ggaatcgagc gcgtgatccc tccgatgttg atcacggctt ggtatccgat
     6601 ccagcaggtg atgccgacgg cgagcaggaa ggcgaactta tcgggtgatt tgaccccggc
     6661 gcgaaagccg cggtaaacga gcccggcaaa caggaagatg acaaccagcc cgccgatcag
     6721 gcccatctcc tcgccgataa tggcaaaaat gccgtcggtg tgggcgccag gaacatagaa
     6781 gaatttctgc ctgcttgcgc ccaaccccaa accatggatg ccgccgctcc caagcgcgat
     6841 cagcagttgc aggatgtgga aaccggttcc ggcggggtcc ttttcgggcg ataggaatga
     6901 agtcaagcgt gccgactgat agccctccac cgagatcatc aagcctgcgc cggctacacc
     6961 cgatagcagc aaggtcagca cgtgcggcag cgccgcaccc gcgacgaaga acatcgtcat
     7021 cgtcgtcagc gtaattacga tcgccgtgcc gaagtcgggt tcgagcagga tcaggccgcc
     7081 caccagcccc accatcatga cgaacggaac gacgcccagc gagaactgct tgacgtggtc
     7141 gcccttggcc gccaaccacg cggatatgta gatcaccatc gccagctttg cgaactcact
     7201 cggctccagt ggtggaagcg gtccgatggc gatccagcga tttgcgccat tgctgcggac
     7261 gccgaagtgc ggcaacaaga cgattagcag cgacaccagg gagaagagca tgatcagcgg
     7321 gctgtagcgc cgcaagtagt cgtacgggac acgctggaaa atcaacagca gcgcgattcc
     7381 ggcgacggcg ctgatcgact gctttgtaat aaagtagttg acgttgtgaa agtcggaata
     7441 gccgatcgca aagctggcgc tgtataccgc caacagccct atgcccgtaa ggatgatcgc
     7501 gagcgcgagg atccagtaat cgggccgtcc ggcatagttt cgccgcccgc gggctacagc
     7561 tgcgatggcg cacctacctt ccgtgcctca ttcagcacga gcctgcgaaa ctcttcgccg
     7621 cggcgctcga aatttggata ggcgtcaaag ctcgtgcagg ccggcgacag cagcacgacg
     7681 tcgccgggtt gtgaccagct tcgcgccatc gatacggctt catcgagcgt tttcacacgc
     7741 tgcacctgtg cgcggggcgc gccgtttgct tcctgccgct cgatcgcgcc ggccagcagc
     7801 cccgcggcct ctccgaagca gataacagca cgtacacgct tgtgaacttc ctcggctaat
     7861 tcatccaatg gtaggttctt ctcacgtcca ccgagcagca atactagcgg ctcttcgaac
     7921 gagcgcagtc cggcaagcgt ccgctcgggc gaagtggcga tgctgtcgtt gtaccaggcg
     7981 gcgccatcga cgttggcaac gtactccaag cggtgcggca ccggtttgaa gctccttacc
     8041 gcatcgccaa atattgtcgg cggaatccgg gcggacgctg ttaccgcagc tgcagcgagc
     8101 acattctcga cgttgtgtct gcccctcaat ggaacttccc cgagggtcac aaatgcggtc
     8161 gtggcatcag cggtgcgcaa aactaactgt tcgtcgctca caaagcaaga gtcctcgcct
     8221 ggcacgtcgg cgttaaacca aaccttcttt gcctgtgtat cgaaggccag acgagcggca
     8281 tccgggttgg tggcgttcaa aacggcgacg tctctgcgcg actggtgtcg aacgaggttt
     8341 cgcttgaggt cgacgtattc gtcccagctg aactggtcga ggtggttcgg cgtcacgttc
     8401 gtaacgcagg cgatgtctgg tccacgttcg aacagctgca gttgcgtgtg gctgacttca
     8461 agcaccgccc agcagttcgg cgccgcggcg tcgagttgtc caagcagcga gccgccgatg
     8521 ttgcccccga cgatatgcgg cactcccgcc gccgcgaacc ccgcctgcac gagggacgtg
     8581 gtggtcgtct tgccgctgct gccggtgatc gcggcgatgg gccccggcca gcgctcgaag
     8641 aactcggtct gcctcgatcg aaccggcttt ccggtggccc gtgcccgcag gaccagcggc
     8701 tctcgatgaa gcagcacgct ctgcgagacg tagatcgcat cacagccgtc ggcggcgtct
     8761 acgggaatag ggccgagtcg tacctccgcg cccagcgcct ccaactcggc cgcgccagcg
     8821 gccaccgcgt tacgcgaccg ggtatcgaat acgatgagcc gcgcgccgcg cgccgcaaga
     8881 aagcggctga ggtcgcggcc ctcgatgccg agtcccagca ccgctacgcg gcggtcgtgc
     8941 agccactcgt cgttcatctt cacagtggtc cagagacctt gagcgccagt gcgacgccga
     9001 tcatggcgcc agcgatgccg accagccaga aacgcatcac gacctgcacc tcagaccacc
     9061 ccatcatctc aaaatggtgg tggagcggcg ccttgcgcag gatccgctta ccgtgggtga
     9121 gcttgaagta accgatctgt agcacgtcgc tgaacgcctc ggcgacgaag acgacgccga
     9181 ttaccggtag tatcagccat tgctcggtca tcaatgcgac caccgcgagg ctggacccca
     9241 gtgcgagcgc acccgtatcg cccatgatca ccatcgccgg ataggcgtta aaccagagaa
     9301 atccgagaat ggcacccacg accgtgtagc aaaacgctcc cagaaacggc tgaccctgaa
     9361 acgcggcgat cgcgccgtaa gccgcaaaag cgaatgcggt cgtgccgccc gccagcatgt
     9421 cggcgccgtc ggtgaccgcc acggcgctgg tagtgccgac gatgatgagg accgacagag
     9481 ggatgattcc ccaaccaatg ttccggtggc cgagccacgg tacggccacg cgatggatgc
     9541 cgagcgcctt gtacaacacc acgcccacga tcagaccgat gatcgcgatc aggccgaact
     9601 ttaatcgcca tgacagcgca tcgcgcaccc gcccctgaag cgtcgcaagg tcgtcgaaga
     9661 agccgattaa tccggttgcc gcgatcgtgc caaacggcaa gagaatcgag tagcgtccaa
     9721 cgatgttaga tgggacagtg acgatcatca cggtgaaaaa gacgatgatg ccacccatgg
     9781 tcggtgtgcc ggccttcgat tgatgggacg gcggcgcaaa ttcgctgatc gccttgccga
     9841 ccttgcgcgt ccgtagctgg ccgataatcg gccctccggc gccggccgca agcaggaatg
     9901 cgatcactcc ggtgacgaaa gctctagaca agggctacgg ctcgatttcg caagtcatct
     9961 tcctgtcgca gggacggacc gctcataacc aggtggtagc gggcgttcgg tgggtacgtt
    10021 catataatcg agtatctctc cggcaagatg actaaacatc ggcgccgtta gggtgccgcc
    10081 gaagtccgca ctcttgaggt tgtcgagctt gacgtaaatc agcacccgcg gattgttgag
    10141 cggtgcgaag ccgatgtatg aggggatcgt cacttcgttg gcataagcgt cgcccgcgct
    10201 gtccttcgtc accagcgtcg gcacgtaggc cgtgccggat ttgccccccg cggtgtagcc
    10261 ggcaatgtta gccagggcgt tcgcttcgag cacctgccgc atcatgtcgc ggagggtcgt
    10321 cgccgtttcg gctgaaatgg cgcggcggcg cacgacaggt gccgtcgttc ttacggttcc
    10381 gttggcgtgg atctctttga cgatgtacgg ctgcatgaga ttaccgccgt tgacgatggt
    10441 ggccacggct gtgaccatct gcagcgccgt aacgctgata ccctgaccga agctgttgga
    10501 ggcgaggtcg gctctcgtcc agtctggctc tgcgttggtg cgtaatatcc cattcgcttc
    10561 tccgctaaat ccggagccgc ttggctcgcc aaagccgaac aggcggacgt acttgtagaa
    10621 gatgtcaggg ccaagcacct tggtcgatag ccataacgta cccaggttga ggctgcgcac
    10681 cagcacctgc gtcatcgtcg tcgtgccgtt ggtgctcagg tcaaaattgt ggaatatgcg
    10741 ttcgttgaca acctcggtgc ctgtgtccag gtaggtcgtg tttgcattaa ccttgccggt
    10801 gtcgatgccc gccgacatcg tgacgagttt gaacaccgac ccgggctcga acatgtttgc
    10861 gacggcgccg ttcttataaa ggggagcctg gttggtatta ttcaggtcga gttgcgagag
    10921 cttgaacgtg ggctcggttg ccatcgccag aattgcaccg gttataggat ccatgacgat
    10981 gacgtttccg cccgtggcgt gggtcttgtc cactccggca tgcagttcgt cttcggccat
    11041 cttctgaatg ttgcggtcaa ttgtcaggac gacatcgcca ccgggctgcg gtggaacgat
    11101 cttacggctg ccaaatgcga tcgggttacc cagcgaatcg cgctcgaact gctcggtgcc
    11161 gggctgtcca gcaagctgct ggttcaggtc gagttccagg ccgctgagac ccgtcccgtc
    11221 gcgacccagg aagccgagca cctgggatgc gatgtcgcca tccgggtaga gcctgaggct
    11281 gtccggggac gcttgcacgc ccttcaacgt tccattgatg gagagggcga tgatttgctg
    11341 accgacttga tattccacgc cgctggcgat tgtgacctgg ccgtttcccc cattacccgc
    11401 gagggcaagc acgtcgtccg gcgtgcgatt cagtagcggc gccagcgcct cggcgccgtc
    11461 gcgagcgact cgcgggtcgc tccataccgc cgggttaatg ataacgtcat acgtactgac
    11521 gctggtggcc aaggggtggc cgttggtatc gagaaggcta ccccggtgcg cgggctcggt
    11581 agccgtgttg acgtgctctt cgctcgcctg agcgcggtaa acgtcgtgct gcaacacctg
    11641 cagatagacg aggcggcaga cgattcctgc ggctgcgagc accaggatga ctgccaccgt
    11701 tgtcagtcgc catgctgccg gttgaagtgg cctcggtgct gtcgccatgt tcgatacccc
    11761 gccgttccgg ttgcgccgct gaggaattgg gagcggcggt gtcggtcaaa tccgctccgg
    11821 ttgcagcgcc ggctaacgca gggataggaa gtgcaagaag tttgcgagca gccctcgttg
    11881 gggaggctta gcgacaaccg gtggctgcgg cacgaagcgt cgcggtaaca gagtttcaat
    11941 gggcgcggct gcgtttatta cgacggattc tgtctggcga ggggcaggca ccattccaag
    12001 ctgagtcgcc tgctgccgaa tgcgctccat ggagctgagc cgcgcaattt ctgcctggat
    12061 gttgtaggtc tgggcgttca gatcggccag ttcctgctgg ttattgcgga tcgcgtatcc
    12121 ggcggtcgtg gcatcactgt cctgcatcac cggcaaaagg gtggcggcga cgatcaatgc
    12181 gaccgcgatc agtagcgaca ctgcggcgcc aaagctgccc agtatcggtc tgttatgtgg
    12241 cgtcgccatc ggtccgtgtg ccaccgtcat gtttgctgac tcccgcgtgt ttattgtttg
    12301 atttagaccc gcacggccgc cctaagcttt gcgctgcggc tgcggggatt tcgccgagtc
    12361 tcgtcttcgc tcggggttat tggcgaacgg ttgatcggcc ggaacgatgc ggtgtgaccg
    12421 caccggcact ccggcaggcc tggtggacaa atacagtcgc gactctcgcg ctgaataaac
    12481 tgtttcacga tccggtcctc gagcgagtga aatgtgatca cagctagccg accgcccgat
    12541 tccaggaggc tgcgagagtc ctccagaatc gatgcgaggt tctccagttc acgatttacc
    12601 gcgatgcgga gcgcctgaaa tgtgcgtgtt gcaggggata cccgctgccc gggtctggcg
    12661 acgacacgct cgatgatgcg ggcgagctgg gtggtggttc gaatggggcg ggaccgcaca
    12721 atagcccgtg cgatgcgccg ggaagcgcgc tcttcgccgt accgccagat cacgtcagcc
    12781 aactcttctt cgccggcgcc attgaccagg tcgtttgccg attctcccgt cgttggatca
    12841 aaccgcatat cgagggggcc ttcggctcga aagctgaagc cgcgttcggg acggtccagc
    12901 tgcagcgacg agacgccgag gtcgaggagc aggccagtca gtggcgtcat gcctgcgtct
    12961 gccgcgacag ctaccgcttc agcgtaattc gcgtggacca gcgcagctcg ggcaccgaac
    13021 cgctgaagcc gcacccgcgc aagcgacagg gcctcgtggt cgcgatcgag accgagcagg
    13081 ctgccgtttg ggctgctttc gtcgaggatg cgctcggcgt gtccgcc
//
LOCUS       DATCPE010000029         3315 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U7219
            k141_167038, whole genome shotgun sequence.
ACCESSION   DATCPE010000029 DATCPE010000000
VERSION     DATCPE010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955033
            Sequence Read Archive: SRR1586279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 16:49:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 640
            CDSs (total)                      :: 631
            Genes (coding)                    :: 627
            CDSs (with protein)               :: 627
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3315
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167038"
                     /isolate="SMAG_U7219"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="VIM45_00980"
     CDS             complement(<1..418)
                     /locus_tag="VIM45_00980"
                     /inference="COORDINATES: protein motif:HMM:NF014047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HEY8819891.1"
                     /translation="MRLTLGDLTNRGAAIAHTDEGQVVFVAYGAPGEEVVATIDRVQK
                     DHLMAHVVEVIVPSRDRVLPLCPYFGACGGCNYQHLTYEAQLEAKRRIVIETLVRIGR
                     LVDVPVQPTVPSPSPWFYRNQARLSCNQRGDVGFTQR"
     gene            623..1495
                     /locus_tag="VIM45_00985"
     CDS             623..1495
                     /locus_tag="VIM45_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012559922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HEY8819892.1"
                     /translation="MNDCTVCLSFDFDQMSGWLFRGNTTPTPLSRGEFGARVGVGRVL
                     DLLKEHGIVATFFTPGHTCDTFPQQCRRIAEAGHEFGHHGYCHEVPATLSRKEEEKIL
                     QKGIEAIVRVAGKRPVGYRSPSWDLSAHSLKLLLQNGFLYDSSLMADDFTPYLCRMGD
                     KFDTKGPYRFGRSVGLVEMPISWLLDDHPHFEWNSQRGSGHAAPSHVEEIWREEFEFM
                     YERVPGGIYTLTMHPQVIGRGHRIRMLDRLIRHMKSRPGVRFAVMNDVAESVRPKLIA
                     QAGLAAGAAQTKKA"
     gene            1500..2588
                     /locus_tag="VIM45_00990"
     CDS             1500..2588
                     /locus_tag="VIM45_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013400425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HEY8819893.1"
                     /translation="MKLFEDMCFGGHEQVVFWSDPGTGLRAIVAIHDTTLGPGLGGTR
                     MYPYASEHEALTDVLRLSRAMTYKNAAAGLDFGGGKAVIIGDSTKDKSEALMHSYGRL
                     VETLNGRYVTTTDVGTTTNDLNIISLETRFVTGTSPAYGGSGDTSMLTGLTVYVGMRA
                     AAKVKWGEPDLAGRTVAVQGTGKVGWHLMENLYAEGAKLIVTDVNREQVQNAASTFGA
                     RVVGTDEIYDVECDIFSPNALGASINYNTVPRLKCEIVCGGANNQLETPEDGEALARR
                     GILYAPDFIVNSGGVINVADESLGYNAERARARGEGVFDTTLRILTQAKERGITPNEA
                     AEAYAVSRIKLLNKVHSTFLPGHSPIPR"
     gene            2618..>3315
                     /locus_tag="VIM45_00995"
     CDS             2618..>3315
                     /locus_tag="VIM45_00995"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY8819894.1"
                     /translation="MRYPHTKRQAELVALATGLARQIAPRARVHDREGTIPVDSYRDL
                     IDAKYQTLTVPSERGGMGVDMIEFVLAQNALAQGDESAALGINMHLMSVANASQPDAW
                     PRELYLRVMYEVVEERALINSAAAEPEMGSPAGGGRPLTMATASKGGWLINGRKTFTS
                     LSPILRYFIVLATIGELPDGGVEIGQFLVRNGPGVRIDPTWDVMGMRSTASHDIVLED
                     AFVPDSDVVSRRSLG"
BASE COUNT          668 a         1021 c         1041 g          585 t
ORIGIN      
        1 ttcgctgcgt gaagccaacg tcgccgcgct ggttgcagct taagcgcgcc tggttgcggt
       61 agaaccaagg cgaaggcgac ggcacagtgg gctgcacagg tacatccacc aggcgcccga
      121 tacgcaccag tgtctcaatc acaatccgtc gtttcgcctc gagctgcgcc tcgtacgtca
      181 gatgctggta attgcaaccg ccacaagcgc caaaataggg gcaaagcggc aaaacgcggt
      241 cgcgcgaagg cacgatcact tcaaccacgt gggccatcag gtggtccttc tgcacgcggt
      301 cgattgtcgc gaccacctcc tcgccgggcg caccgtaagc gacgaacaca acctggcctt
      361 cgtcggtatg agcgattgcg gcgccacggt ttgtgagatc accgagcgtc agccgcaact
      421 gaacgccctg ccgatatccg ggacgggtcg tgcgagagga ggtcctgttc gtttcaacgg
      481 gaatcatgca ctgagtgtac ggtggcaccg cgccaggaca aacgtcgggg tagtccggca
      541 gcccggtcgc tacttgacgg cgattcgggg gcggcggatc gtgtagacag atggcgcggg
      601 gccacttcgg gaggcaatga gcgtgaacga ctgcaccgta tgtctctctt tcgacttcga
      661 ccagatgtct ggatggctct tccgtggaaa cacgactccg acaccgctgt cccgcggcga
      721 attcggggcg cgggtcggtg tcgggcgtgt tctcgacctt ttgaaggagc acggcatcgt
      781 cgcgacgttt tttacccctg gccacacttg cgacaccttt ccccaacaat gccgccgcat
      841 cgccgaggca ggccacgagt ttggccatca cggttactgc cacgaagtgc cggccacgct
      901 gtcccgaaaa gaagaggaga agatcctgca gaaaggcatc gaggcgatcg tgcgggtagc
      961 aggcaagcgg ccggtcggtt atcggtcgcc gtcctgggat ctgagcgctc attcgctaaa
     1021 actgctgctg cagaacggct tcctttacga cagcagcctg atggccgacg actttacgcc
     1081 gtacctttgc cgcatgggag acaaatttga taccaagggt ccctaccggt tcggtcgctc
     1141 ggtggggctg gtcgaaatgc ccattagttg gttgctcgac gaccatccgc acttcgagtg
     1201 gaactcacag cgcggcagcg gccacgccgc tccctcgcac gtcgaggaga tctggcggga
     1261 agagttcgag ttcatgtacg agcgcgttcc aggtggcatt tacaccttga ccatgcaccc
     1321 tcaagtgatt ggccgcggtc accgaatccg catgctcgac cggctgattc ggcacatgaa
     1381 gtcgcgaccc ggcgttcgct ttgccgtgat gaacgacgta gccgagagcg tgcggccgaa
     1441 gctgattgcc caagcagggc tggcagcggg cgccgcacaa acgaagaagg cgtagcccga
     1501 tgaagctgtt cgaagacatg tgcttcggcg gccacgagca ggtcgtcttt tggagcgacc
     1561 ctggtacggg gctgagagcg atcgtcgcga ttcacgacac gaccctcggt ccggggctcg
     1621 gcggaactcg aatgtatccc tacgcgagcg aacacgaagc cctgaccgac gtactccgcc
     1681 tgtcgcgggc gatgacttac aagaacgctg ccgccggact tgatttcggc ggtggtaaag
     1741 ccgtaatcat cggcgattcg acgaaagaca aatccgaggc gctgatgcat tcgtatgggc
     1801 ggctggtcga gaccctcaat ggccgttacg tcaccaccac ggacgtaggc acgacgacca
     1861 acgacctgaa cattatttcg ttagagaccc ggttcgtgac cggcacgtcg ccggcatacg
     1921 gcggcagcgg cgacacttcg atgctgaccg gcctaacggt ctatgtcggc atgcgggccg
     1981 cagcaaaggt aaaatggggc gagccggacc tcgccggccg tacggtagca gtacagggga
     2041 caggcaaagt cggctggcac ctgatggaaa acctgtacgc ggaaggcgcg aagctcatcg
     2101 tcaccgacgt caaccgcgaa caggtacaaa atgccgcttc aacgttcggc gcccgcgtcg
     2161 tcggaaccga cgagatctac gacgtggagt gcgacatctt ttcgccgaac gcgctcggcg
     2221 cctcgataaa ttacaacact gtgccgcggc taaagtgcga gatcgtctgc ggcggcgcca
     2281 acaaccagct cgagacgccg gaggatgggg aagctcttgc gcgccgcgga atcctttacg
     2341 cgcccgactt cattgtgaat tcgggcggcg tgatcaacgt cgcggacgaa tcgctgggtt
     2401 ataacgccga gcgcgcccgc gccaggggcg agggcgtatt cgatactacg ttgcgcatcc
     2461 ttacgcaggc gaaagagcgc ggcatcacgc caaacgaagc ggctgaagca tatgcggtgt
     2521 ctcggatcaa gctgctgaac aaggtgcata gcactttttt gccgggccac tcacccattc
     2581 cccgttaacc gcggcagggc cgcgcaggca ggaacccatg cggtacccgc acaccaagcg
     2641 gcaggcggag cttgtcgcac tcgctaccgg cctggcacgc cagattgcgc cccgcgctcg
     2701 cgtgcacgac cgcgaaggca caatccccgt cgatagctac cgcgatctca tcgacgcgaa
     2761 gtaccagacc ctcaccgtcc cgtccgaacg aggcggcatg ggcgtcgaca tgatcgagtt
     2821 cgtgctggca cagaacgcgc ttgcccaagg agacgaatcc gccgcgctgg gcataaacat
     2881 gcacctgatg tccgtagcca acgcgtcgca gccggacgcc tggccgcgag agctgtacct
     2941 gcgtgtcatg tacgaggtcg ttgaggaaag ggcgctcatc aattcggctg ctgccgagcc
     3001 ggagatgggc agcccggcgg gaggcggcag gcccctgacg atggctacag ctagtaaagg
     3061 cggttggctc atcaacggcc gcaagacgtt caccagcctg agccccatcc ttcggtactt
     3121 catcgtgctt gcaacgatcg gcgagctgcc cgatggcggc gtcgagatcg gccagttcct
     3181 ggtgcgcaat ggacccggcg ttcgcattga cccgacctgg gatgtcatgg gcatgcgctc
     3241 gacggccagc cacgacatcg tgctcgagga cgcattcgtg cccgattcgg atgtcgtttc
     3301 ccgccgctcc ctggg
//
LOCUS       DATCPE010000030         1847 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U7219
            k141_231856, whole genome shotgun sequence.
ACCESSION   DATCPE010000030 DATCPE010000000
VERSION     DATCPE010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955033
            Sequence Read Archive: SRR1586279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 16:49:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 640
            CDSs (total)                      :: 631
            Genes (coding)                    :: 627
            CDSs (with protein)               :: 627
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231856"
                     /isolate="SMAG_U7219"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..615)
                     /locus_tag="VIM45_01000"
     CDS             complement(<1..615)
                     /locus_tag="VIM45_01000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEY8819895.1"
                     /translation="MVMNLGLNPLTSPEARDNPYPFFAMARQMMPVLRLEQFGVWSAF
                     LYEDCRNILRDSKRFSSSFIRRMPEGQARPRPSMLNSDPPRHTRLRELVNKAFTPRMV
                     AQLEPRVRSITDELVDAVLPTGSMDLIDDLAYPLPVIVIAEILGVPPSDRAMFKHWSD
                     TLVATLGAGFSTTPDRAPTETIQELNEYFTRVIEQRRQEPREDLI"
     gene            complement(649..1680)
                     /locus_tag="VIM45_01005"
     CDS             complement(649..1680)
                     /locus_tag="VIM45_01005"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY8819896.1"
                     /translation="MHSRYNEHTRDESGIAASEGPLMLLQHLETFVRVVEEGSFTRAG
                     ELLNVSQPAVTRQIAALERELGAPLVERGGRGLHLTTVGQTAYAHARHILAQVEHLRS
                     DVSSLSDPQRGEVSIACVTTVGLFTLPRLLADYRAAYPEVRLRVWSARADGVLDRLLE
                     GNADLGLTSTPVAHPRLISTPLFEDPVIPVAAPQFAAALPDPLPLEQLAELDMILFQA
                     PSRFRTHVDAELEQAGVYPRITMEFDSHEAVRTAAQMGYGVGIVPRQAVAAELAGGQL
                     VELHVAGLPEIKRTTCLVERRGNIHLPPAANFIKLLLERYGGAAGVAKTRSPFAAPAS
                     RLAASGRQV"
BASE COUNT          352 a          553 c          624 g          318 t
ORIGIN      
        1 gatcagatcc tcgcgcggtt cctggcggcg ctgctcgatc acgcgggtga agtactcgtt
       61 cagctcctgg atcgtctccg ttggcgcccg gtcgggagtc gtgctgaagc cggcgccgag
      121 ggtggcgaca agcgtgtcgg accagtgctt gaacattgcg cggtcggatg gcggcacgcc
      181 gagaatctcc gcgatcacga tgacaggcag cgggtaggcg agatcgtcga taagatccat
      241 ggaaccggtt gggaggaccg catcgaccag ttcgtccgtg attgatcgaa cgcgcggctc
      301 gagctgcgcc accatgcgcg gtgtgaacgc tttgttcacg agctcgcgca gccgtgtgtg
      361 ccggggaggg tcggagttga gcatgctggg ccgcggcctt gcctggcctt ccggcatgcg
      421 gcggatgaac gacgaggaga agcgcttgga atcgcgcagg atgttgcgac agtcctcata
      481 caggaacgca ctccagacgc cgaactgttc gagccgcagc accggcatca tctgccgcgc
      541 catggcgaaa aacgggtatg gattatcgcg cgcctccggc gaggtgagcg ggttcagtcc
      601 gaggttcatg accataacgg acgcctcctg ggacgactta caccagcatc agacctgacg
      661 tccgctcgcg gcaagtcgcg aagccggtgc tgcaaaagga gatcgcgttt tagcgactcc
      721 ggcggcgccg ccgtaccgtt ccaacagaag cttgatgaag ttcgcggcag gcggcaggtg
      781 tatgttgccg cgacgttcaa ccaggcaggt cgtccgctta atctcgggca atcccgccac
      841 gtgtagttcc accaactgcc cgccggctag ctcggctgca accgcctgtc gcggcacaat
      901 gccgacgccg tagcccatct gcgccgcggt gcgtaccgct tcatggctgt cgaactccat
      961 ggtgattcgg ggatacacgc ccgcctgttc caactcggcg tccacgtggg tacgaaaacg
     1021 cgaaggcgcc tgaaaaagga tcatgtccaa ctcggcaagc tgttcgagcg gcaaggggtc
     1081 gggaagggcg gccgcaaact gcggggccgc caccgggata accgggtctt cgaacagggg
     1141 cgttgagatc agacgcgggt gcgcgaccgg cgtactggtc aagcccagat cggcgttgcc
     1201 ctcgagcagc cggtccagca cgccgtcggc gcgcgccgac cagacgcgca gacgtacctc
     1261 cgggtaggcg gcgcgatagt cggcaagcag acgaggcagc gtgaacaggc ccacggtcgt
     1321 cacgcaggcg atcgatacct cgccccgctg ggggtcagat agcgacgaaa cgtcgcttcg
     1381 caggtgctct acctgcgcga ggatgtggcg agcgtgtgca tacgccgtct ggccgacggt
     1441 agtcaggtgt aagccgcgtc cgccgcgttc gaccagcggg gcgccaagct cgcgctccaa
     1501 cgcggcgatc tggcgcgtca cagcgggctg actgacgttg aggagctcgc cggcgcgggt
     1561 aaagcttcct tcttcaacga cccttacgaa cgtttcgagg tgctgcaaca gcatgagcgg
     1621 tccctccgaa gcggcgattc ccgattcgtc acgcgtatgc tcattatagc gactatgcat
     1681 tggacgtatg gcacatggag aggtacgtta agcacaagat gactggaatg aatcggacag
     1741 gagggggcgt catgctccgc gagatcgtct ggaagatcgg tggcgaacag ggccaggggc
     1801 tcgaccccac cagtgacgtg ttcgcaacgg tttgtaatcg aatgggc
//
LOCUS       DATCPE010000031         4176 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U7219
            k141_4741, whole genome shotgun sequence.
ACCESSION   DATCPE010000031 DATCPE010000000
VERSION     DATCPE010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955033
            Sequence Read Archive: SRR1586279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 4176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 16:49:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 640
            CDSs (total)                      :: 631
            Genes (coding)                    :: 627
            CDSs (with protein)               :: 627
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4176
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4741"
                     /isolate="SMAG_U7219"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="VIM45_01010"
     CDS             <1..219
                     /locus_tag="VIM45_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012595060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEY8819897.1"
                     /translation="LKTPPASDLLKKAAGIEKGGKQQKKEKVGSVTQAQIREIAGIKL
                     KDLNAYDIESAMKMIAGTARNMGIDVTS"
     gene            313..1026
                     /gene="rplA"
                     /locus_tag="VIM45_01015"
     CDS             313..1026
                     /gene="rplA"
                     /locus_tag="VIM45_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEY8819898.1"
                     /translation="MPKHGKKYLDVAKQVDNDKFYDPAEGIALAKQAHFAKFDETVEL
                     HLRTGLDPRHADQQLRGTTLLPHGLGKTVRVLVFAEGDAARAAEEAGADFVGGDDLIT
                     RVAGGFTDFDVALSQRELMGKVGRLGRVLGPRGLMPNPRSGTVVEPQDMAKAVQDAQR
                     GRVEFRLDKTAIIHVPIGKVSFEETALAENLANLIDAVMKAKPAGAKGVYIRSATLTS
                     TMGPGIHLDLAPTLALKPA"
     misc_feature    1118..1281
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            1306..1839
                     /gene="rplJ"
                     /locus_tag="VIM45_01020"
     CDS             1306..1839
                     /gene="rplJ"
                     /locus_tag="VIM45_01020"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEY8819899.1"
                     /translation="MPTARKEAEVAELQDLVSRAAISISADYRGLSVAEMTALRRRMR
                     DAGVDVRVVKNTLLKLAAERAGQPKVAEIATGPTAIVFGYGDIAVPAKALQEFIRVSR
                     STLTLNAAYVDGEILRGAAITDLAALPSKDQLLANFIGALRSPIAAFASLLSGTIQSF
                     AGLIDARAQQLEASAAA"
     gene            1904..2296
                     /gene="rplL"
                     /locus_tag="VIM45_01025"
     CDS             1904..2296
                     /gene="rplL"
                     /locus_tag="VIM45_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012459032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEY8819900.1"
                     /translation="MPSDRVQQVLDIIKEMTILELRDLNKQIEEVFGVTAAAPMMMAA
                     GAAPAGAAATAEPVEEQTEFSVVLTSFGENKINVIKAVREVTSLGLKEAKDLVESAPA
                     PIKEGVSKDEAEQVRAKLANAGASVEVK"
     gene            2387..2989
                     /locus_tag="VIM45_01030"
     CDS             2387..2989
                     /locus_tag="VIM45_01030"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEY8819901.1"
                     /translation="MPIAMPDDEPRSCSFCNQFADDSCRRCGRAYCKAHGRELCNVCL
                     SPSSALPSPAVYKGSLITLAIAVVAGLLVLIHPLSLPGEHRLAKGSGQNPALLQPGTA
                     PGNRTSGSVVPTPLPGVAPNSTSPALRSYTVVLNDTLTGIAQNFGTTVVAIQAANPGV
                     NESNLKAGQELLIPPSEGAARPLGSPSPAAALSATPTSTP"
     gene            3032..3571
                     /locus_tag="VIM45_01035"
     CDS             3032..3571
                     /locus_tag="VIM45_01035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8819902.1"
                     /translation="MLDLSPRGRVMRDALIYTPLFLVCVAGILLMVGSGDPPWIGVFL
                     VGVMAFLFGYQSIQSLRDLSSAPLTVEGPITRRWTKRDAFVSKSHYITVERGIYRIPV
                     EDYLDLLEKDRVRVVAYPHTGTVVSVERLARPEDEPKPAGAMAKGRLRMLRTARSTPS
                     VRRGEAVDAPPSPDEGRRH"
     regulatory      3643..3756
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3858..>4176
                     /locus_tag="VIM45_01040"
     CDS             3858..>4176
                     /locus_tag="VIM45_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012692406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HEY8819903.1"
                     /translation="MEDILTIAGARFHSRLMLGTGRNRTVDEMLAALEASGTQIVTVA
                     IRRLDLDNPNEKTLLDYIDWGRFQVLPNTAGSRTADEAVFAARLGRSVTGSDWVKLEV
                     IPDA"
BASE COUNT          825 a         1307 c         1323 g          721 t
ORIGIN      
        1 ctgaaaacgc caccggcgtc cgaccttctg aagaaggcag cgggcatcga aaaaggcggc
       61 aagcagcaaa agaaagagaa agtcgggagc gtaacgcagg cgcagatccg tgagatcgcg
      121 ggcatcaagc tcaaggattt gaacgcctat gacatcgagt cggcgatgaa aatgattgcc
      181 ggcaccgcac gcaatatggg tatcgacgtc acttcctagc accccactcg gggtcatccc
      241 ggtgtatata cctccgccga caagcgagag ggagggctgg gcgcattccg cgatgcccga
      301 aggagggtca caatgccaaa gcacggcaag aagtatctgg acgtagccaa gcaggtcgac
      361 aacgacaagt tctacgatcc ggccgaaggg atcgcgcttg caaagcaggc gcattttgcc
      421 aagttcgacg agacagtcga actgcatttg cgcacgggac tggatccacg gcacgccgac
      481 cagcagttgc gtggcaccac actgctgccg cacggcctgg gcaagacggt gcgtgtgctc
      541 gtcttcgccg aaggcgacgc cgcacgcgcg gccgaagagg cgggcgccga cttcgttggc
      601 ggcgacgacc tcatcacccg cgtagcggga ggcttcacgg acttcgacgt cgcgctttcc
      661 cagcgcgagt tgatgggcaa ggttggccgg ctcgggcggg tgcttggtcc gcgcggcctg
      721 atgccgaacc cgcgttccgg cacggtggtc gagccgcagg acatggccaa agcggttcag
      781 gacgctcagc gcggccgcgt cgagttccgg ctcgacaaga ccgcaattat ccatgtgccg
      841 atcggcaaag tcagctttga agaaacggcg ctggccgaaa acctggcgaa cctgatcgac
      901 gccgtgatga aggcgaagcc ggccggcgcc aagggcgtgt acatccggag cgctaccctc
      961 acttcgacga tgggcccggg cattcatctc gatctggcgc caacgcttgc gttgaagccg
     1021 gcttaagaga tcgagggcac taccagaccg agctttcgag cccgctgcgt ttgaccgacg
     1081 cggcgggctt cagagatact caattggcaa attgaatact gtatctgtca cgtagccgta
     1141 gacagccggc ggccttcagg ccttaatcca ccagccagcc gaggcgagcg accgagcgaa
     1201 catccgcgat ggcacgactg gtgccgcggt acagcgtttc gagccctcgc accgacgtgc
     1261 gagggctttt ttggtggccg ccggatggaa gaaagtggtg tgtgagtgcc aacagcacgc
     1321 aaggaagccg aggtcgcgga gctacaggac ctggtgtcgc gcgctgctat ctcgatcagc
     1381 gccgactatc gcgggcttag cgtcgcagag atgacggcgc tgcgacgccg tatgcgcgat
     1441 gccggcgtcg atgttcgcgt cgtcaagaac acgctgctga agctcgccgc ggaacgggcc
     1501 ggacagccga aggtggctga gatcgccacc gggccgaccg cgatcgtatt tggctatggc
     1561 gacatcgcgg tgcccgccaa ggcgctgcag gaattcatcc gcgtgtcacg cagtaccctc
     1621 actctcaacg ctgcgtacgt ggatggcgag atcctccgcg gagcagcgat caccgacctg
     1681 gccgccctgc cctcgaaaga tcagttactt gccaacttta tcggcgccct gcgatcgccg
     1741 atcgcggcct ttgcgagcct gctctcgggg acgatccaga gcttcgcggg tctcatcgac
     1801 gcccgcgccc aacagcttga ggcaagcgct gccgcctgag cggagcgctg gaattttgag
     1861 taggtccggc tcaaacgccg gaatcaggaa aaaggagcgt tcaatgccca gcgatcgcgt
     1921 acagcaggtg cttgacatca tcaaagagat gactatcctc gagctccgcg acctcaataa
     1981 gcagatcgaa gaagtattcg gcgtcacggc cgctgcgccg atgatgatgg ccgcgggcgc
     2041 ggcaccagcc ggcgccgccg caacggccga gccggtcgaa gagcagacag agttctcggt
     2101 cgtgctaacc agcttcggcg aaaacaaaat caacgtgatc aaggcggttc gagaagtgac
     2161 gtccctggga ctcaaagagg ccaaagacct ggttgagagc gcacctgctc cgatcaagga
     2221 aggtgtcagc aaggacgagg cagagcaggt ccgcgccaag ctcgcgaatg ccggcgcctc
     2281 agtcgaggtc aagtagaccc gccccgaaga ggtgcaatga gcggggatgg gccaacaccc
     2341 atccccgctt ttgcgcgccc gtcgatagtt aaattgttat gctataatgc cgatcgcgat
     2401 gccggacgat gagccccgct cttgttcctt ttgtaatcaa tttgcggatg acagctgccg
     2461 gcgctgcggc agggcttact gcaaggcgca cggtcgcgag ctctgcaacg tctgcctctc
     2521 cccatccagc gctctgcctt cgccggcggt gtacaagggc tcgctgatta cgctggcgat
     2581 tgccgtggtc gccgggctat tggtgctgat tcacccgctc tcgcttcccg gcgaacaccg
     2641 gcttgcgaag ggatcgggcc aaaacccggc actgttgcag cccggtacag cgccgggcaa
     2701 ccgcaccagc ggctcggtgg tgccaacgcc gttgcctggc gtggcgccaa actcgacctc
     2761 gccagccttg cgctcgtaca ccgtggttct aaatgatacg ctgaccggga tcgcgcagaa
     2821 cttcggcacg accgtcgtcg cgatccaggc tgcgaatccc ggtgtgaacg agagcaacct
     2881 gaaagctggc caggagttgc tgatcccgcc ctctgaagga gcggcgcgtc cgttgggcag
     2941 cccgagtccg gcggccgctc tctcggcaac tccgacgtcg acaccgtaaa gcggccagca
     3001 aagtctggac ggatcacgca aagcaggacc tatgctcgac ctcagtccgc gcggcagggt
     3061 tatgcgggat gcactgatct atacgccgct tttcctcgtg tgcgtcgccg gaattttgct
     3121 gatggtcggg agcggagatc cgccgtggat cggcgtgttt ctcgttggcg tcatggcgtt
     3181 tctgttcggc taccaaagta tccagtcgtt gcgcgacctc tcgtcggcgc cgctcacggt
     3241 cgagggcccg attacccgcc gctggaccaa gcgcgacgct tttgtttcta agagccacta
     3301 catcaccgtg gagcgcggca tttaccgcat ccctgtagaa gactatctgg atctgctcga
     3361 aaaggaccgc gttcgggttg tcgcatatcc ccacaccggg acagtggtat cggtcgagcg
     3421 cctcgcgcgc ccggaggacg agccgaaacc cgccggcgct atggccaagg gccggctgcg
     3481 catgctgcgg accgcccgca gtacgcccag cgtccgtcgc ggcgaagccg tcgatgctcc
     3541 tccatccccc gacgagggac gccgtcactg accgaattcg cgccctctta ttgacgcctt
     3601 tcgccgggcg ctgatacgat tagtcgggta cactcaaaac cgcatagcgc tcacgggtgc
     3661 tcggggaagc cgggctgaga gggtggccgc cgatcgccgc cgaccgttgg acctgatccg
     3721 ggtaatgccg gcgtagggaa aacgatctga tcacgcagca gtgccgcatc ctctctttcc
     3781 gccgcccgct aaccgggcgg cgttttgtat tcggcccgct ctgcccgcgg cgcggctgaa
     3841 gaggaaggct gactcgcgtg gaagacattc taaccatcgc cggtgcgcga tttcactctc
     3901 gactcatgct tggcaccgga cgcaaccgga ccgtcgacga aatgctggcc gcgctggagg
     3961 cgtccggcac acagatcgtc accgtcgcca tacgccgcct ggacctggac aatcccaacg
     4021 agaagacgct gttggactac atcgactggg gccgcttcca ggtgctgccg aacacggccg
     4081 gcagccgaac cgcggacgag gcagttttcg ccgctcgact gggtcgttcg gtcacgggat
     4141 cggactgggt aaagctggaa gtgatccccg acgcca
//
LOCUS       DATCPE010000032         3138 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U7219
            k141_492136, whole genome shotgun sequence.
ACCESSION   DATCPE010000032 DATCPE010000000
VERSION     DATCPE010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955033
            Sequence Read Archive: SRR1586279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 16:49:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 640
            CDSs (total)                      :: 631
            Genes (coding)                    :: 627
            CDSs (with protein)               :: 627
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3138
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_492136"
                     /isolate="SMAG_U7219"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(64..978)
                     /gene="prmA"
                     /locus_tag="VIM45_01045"
     CDS             complement(64..978)
                     /gene="prmA"
                     /locus_tag="VIM45_01045"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEY8819904.1"
                     /translation="MDWVELSAEVSGDRVEDLCSALENVAPGGCSIEDPIVPLGPEEG
                     VRREPWRPSTVRIYLPVDVHIDERRRDAAVAAARQSVRFRERPVREADWANAWKEFFQ
                     VEQVGRHIVVRPTWREFSPSPEDVVLHLDPGMAFGTGQHETTRMCLVVLEELAEPGMR
                     VLDVGCGSGILSVAAARLGAASVTAVDLDSVAVEATAVNAARNGVAAIVQVAEGSLDD
                     GWPFGAMPSAEFDLVLANIHAAAAETLALAYWHALRPGGTLVASGMIAERLDGACAAL
                     SNNGFDRLDVRVLGEWRTVVARRPDASG"
     gene            complement(990..2108)
                     /gene="dnaJ"
                     /locus_tag="VIM45_01050"
     CDS             complement(990..2108)
                     /gene="dnaJ"
                     /locus_tag="VIM45_01050"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEY8819905.1"
                     /translation="MATQQKDLYEVLGVPRTATAEEIKKAFRKLAMQYHPDRNSDSGA
                     EERFKEINTAFEVLSDPEKRAAYDRFGHAGIGQQGPSGFEGFSNFGGFGDIFDAFFGG
                     ATRQRRGPARGPDLRFNLTLKFEEAVFGAEKEIDVSRTEQCAMCGGSGAEPGSKPERC
                     PSCNGTGEIRRVQQSIFGQFVNVTQCDRCHGEGRIITSPCAQCRGAGRERKVRKLQVT
                     VPPGVDNGQQLRLSAEGEAGVQGGPPGNLYVLLHVQEHDLFKRDEDDIILDLPVNVSQ
                     AALGDEVQIPTIDGKAQLKIPAGTQSGRIFELKGNGVPHLRGGGRGDQIVRVRVVTPT
                     NLTLEQKRLVSELGKSLGTATMPHEEKGFFDRIKDAFG"
     gene            complement(2262..>3138)
                     /locus_tag="VIM45_01055"
     CDS             complement(2262..>3138)
                     /locus_tag="VIM45_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012882438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEY8819906.1"
                     /translation="EAVKKFFGKEPHKGVNPDEVVAIGAAIQAGVLRGEVKDVLLLDV
                     TPLTLGIETLGGVMTPLIQRNTTIPTSKSQVFSTAADGQSSVEIHVLQGERAMAADNK
                     SLGRFILDGILPAPRGVPQVEVTFDLDANGIVNVSAKDKGTGKEQKITITASSGLSKE
                     EVEKLQREAEMHADEDRKRREEIEMRNNADNLAHQAEKLLRENGDKIPAELKSEVEGK
                     AETLRNALKGDDAEAIRRDFESLSEALQKVGAAVYGAAGPGAGAGFGEDGRSANGHAE
                     GKPEEGTVEGEFREV"
BASE COUNT          522 a         1038 c          933 g          645 t
ORIGIN      
        1 gtcatgatgg ccgctctatt attcaatcga aggtccggca cgtggaaacg gtgcatggct
       61 caactaacct gaagcatcgg gacgccgagc tacgacggtg cgccactcgc cgaggacgcg
      121 gacgtcgagc cgatcaaagc cgttgttcga aagtgcggcg caggcgccat ccaacctctc
      181 tgcaatcatg ccgctggcaa cgagcgttcc gccgggacgg agtgcatgcc agtaggccag
      241 cgccagcgtt tccgcggccg cggcatgaat attggccagg acgaggtcga attcagcgct
      301 gggcatagct ccgaaaggcc aaccgtcatc caggctcccc tcggctactt gaacaatagc
      361 agccacgcca ttgcgggctg cattgacggc ggttgcctcg acggccacgg aatcgaggtc
      421 caccgcggtg acggacgccg caccaagtct ggccgccgca acagagagga taccggatcc
      481 gcatcccaca tcgaggacgc gcattcccgg ttcggccaac tcttcaagca ccacgaggca
      541 catgcgggtg gtttcgtgtt gccccgtgcc gaaggccatg ccgggatcga ggtggagcac
      601 gacgtcttcg gggctcgggg agaattcacg ccacgttgga cgcaccacaa tgtgccggcc
      661 gacctgttca acctggaaga actctttcca ggcgttcgcc caatcagcct cgcgcaccgg
      721 ccgctccctg aaccgtaccg attgccgcgc ggcggccacc gcggcgtcgc gacgtctttc
      781 atcgatgtgt acgtccacgg gcaggtagat tcggactgtc gatgggcgcc agggctcgcg
      841 ccgaacgcct tcctcggggc caagcggcac gatggggtct tcgatactgc agccgcccgg
      901 cgccacattc tcgagcgccg agcacagatc ctcgacccgg tcgcccgaca cctccgcgga
      961 gagttcaacc caatccatca gggtcccgct tacccgaacg cgtccttgat gcgatcgaag
     1021 aagcctttct cttcgtgcgg catcgtggcc gtgccgagtg atttaccaag ttcgctgacg
     1081 aggcgcttct gttccagggt gaggttggtg ggagtgacca cgcgtacgcg cacgatttgg
     1141 tcgccgcgtc cgccgccgcg caggtgaggg acaccattgc ccttcaattc gaatattcgg
     1201 cccgactgcg ttccggccgg gatcttcaac tgcgccttgc cgtcgatggt tgggatctga
     1261 acctcgtctc cgagcgctgc ctgggaaacg ttgaccggca gatcgaggat aatgtcgtcc
     1321 tcgtcccgct tgaacaggtc gtgctcttga acgtgtaaga gcacatacag gtttccgggt
     1381 ggtccccctt gcacgcccgc ttcgccttcg gcggagagcc gaagctgctg gccattgtcc
     1441 actccgggtg gaacggtgac ctgcagcttg cgtactttac gctcgcgtcc tgccccccgg
     1501 cactgggcgc aggggctcgt gatgattcgg ccttcgccat gacagcggtc gcactgtgtg
     1561 acgttcacaa actgaccgaa aatgctctgt tgaacgcggc ggatttcgcc cgtgccgttg
     1621 cagctagggc atcgttccgg cttgctgcct ggctccgcgc cgctgccgcc gcacatcgcg
     1681 cactgctctg tgcggctgac atcgatctcc ttctctgcgc cgaagacggc ctcttcgaac
     1741 ttgagggtca ggttgaagcg cagatcgggc ccgcgcgccg gtccgcgtcg ctgacgtgta
     1801 gcgcccccga agaaggcgtc gaagatatcg ccgaagccac cgaagttact gaacccctcg
     1861 aagccgctcg gtccctgctg gccgatgccc gcgtgaccaa agcgatcgta tgcggcccgt
     1921 ttctccggat cggatagaac ttcaaaggca gtattgatct ccttgaatcg ctcctcggca
     1981 cccgaatcgc tgttgcgatc ggggtggtac tgcatggcca gcttgcgaaa ggccttcttg
     2041 atctcttcgg ctgtcgcggt gcggggaacg cccaacacct cgtagaggtc tttctgctgc
     2101 gtggccatct tcctctttat ccatccttgg tcggggtcgg aactagccgc ctcattctac
     2161 cagtaccgtt tcgacctgag cgcggcgtcg ggcgatcagg cccaggccgc ggggtttcga
     2221 aagagcccca cgactagatt tccctgcatc cttcccgcga tctagacctc ccggaattcg
     2281 ccctcgacgg tgccttcctc gggctttcct tcggcgtggc cgttggcgct ccgaccgtct
     2341 tctccgaagc cggctccggc ccccgggccc gccgcgccat aaacagccgc gccgactttc
     2401 tgaagagctt ccgaaagcga ttcaaagtca cgccgaatgg cctcagcgtc gtcgcctttg
     2461 agcgcgttgc gcagcgtctc tgccttgcct tcgacctcgc tcttcagctc agccgggatc
     2521 ttatcgccgt tttcacgcag taacttctcc gcctggtgcg ccaggttgtc ggcgttgttg
     2581 cgcatttcga tctcttcgcg acgcttgcgg tcttcatccg cgtgcatctc ggcttcgcgc
     2641 tgcagcttct cgacctcttc tttcgagagg ccagacgaag cggtaatcgt gatcttctgc
     2701 tccttaccgg tgcccttgtc cttggccgag acgttcacga tgccgttcgc gtccaggtcg
     2761 aaagtaactt cgacctgggg tacgccgcgc ggcgcaggca gaatgccgtc gaggataaag
     2821 cgaccgagag acttgttgtc ggccgccatt gcgcgttcgc cctgcaggac gtggatctcc
     2881 acgctcgact ggccatcggc cgcagtggag aacacctggc tcttactggt cgggatcgtt
     2941 gtgttgcgct gaatcagcgg cgtcatcaca ccgccaagcg tctcaatgcc cagcgtcagc
     3001 ggggtcacgt cgagcagtag tacatccttg acttcgccgc gcaagacgcc cgcctggatc
     3061 gcggcgccga tggcgacaac ttcgtccggg ttgacgccct tgtgcggctc cttgccgaag
     3121 aacttcttga ccgcctcc
//
LOCUS       DATCPE010000033         3464 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U7219
            k141_200150, whole genome shotgun sequence.
ACCESSION   DATCPE010000033 DATCPE010000000
VERSION     DATCPE010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955033
            Sequence Read Archive: SRR1586279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 16:49:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 640
            CDSs (total)                      :: 631
            Genes (coding)                    :: 627
            CDSs (with protein)               :: 627
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3464
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200150"
                     /isolate="SMAG_U7219"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..618)
                     /locus_tag="VIM45_01060"
     CDS             complement(<1..618)
                     /locus_tag="VIM45_01060"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY8819907.1"
                     /translation="MSGALDGIRVIDLAQGIAGPYGAMLLAEQGADVLKIEPPEGDPY
                     RTQPGFRALNRSKEGAVADLETEQGRAAILDAVAAADVLVYDGSPGDVARLGLDYASL
                     SAINRRLVYCWMPPYGSCGPHAERAPDDALVAALDGIMATQYSSHPGPTYLTVPLSSY
                     GAAILAAGAICAALWVRQRTGQGQLVEVSWLAGGFAVQTGTILMGE"
     gene            complement(629..1447)
                     /locus_tag="VIM45_01065"
     CDS             complement(629..1447)
                     /locus_tag="VIM45_01065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8819908.1"
                     /translation="MSTTWIRVSFAVLLGLVLAVSAGFGVLTFESGPRPPQPVGLSFS
                     QLNGNSTDQDSTRINKQIDGFYSDNQTYREKFPDHQRNIFLWFAGIGIVLGVIGVALP
                     RVVNYLRLGFVFGGLLLVGAGTWYTLQQVPQAAPPASSLLALFSGGSPKELDTAGRFL
                     RFAVSIVGLLVLLFVGLWRLTDWQPAGASEGRQAPGAIMGGYAPGAAPMLTPYGSPAG
                     APTRPSEEPAKWARPDDLIQTTQPPAPPVVPTQPSAPAGSEEIARTTESSAPPL"
     gene            1808..2629
                     /locus_tag="VIM45_01070"
     CDS             1808..2629
                     /locus_tag="VIM45_01070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8819909.1"
                     /translation="MSLAQRVLKISTVAAAVLSIAAISGSASGVLAQSTPPVARIFGS
                     ITFTGSTNLNWATVTAYSGSTTCGTGSADGRYVDIDSGNPGCNRVGTVITFKVGSQWA
                     NQTFVIPDLPGSAQQVNLTGPAASQPGGGGAATSSATYSPGWNLAGGPAGTLFSQAAG
                     VLYTFPAGASAYTQVPNTQPAQAGVGYWAFFNSQTTVTLSGSTSLPFTVTAPAGQYIM
                     IANPSATSTVTVSGADVVYTYDPTNAASPYSAVSTLRPGQGAWVYSAAGGTITMQ"
     gene            complement(2733..>3464)
                     /locus_tag="VIM45_01075"
     CDS             complement(2733..>3464)
                     /locus_tag="VIM45_01075"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEY8819910.1"
                     /translation="YFTGAFADFYYLRPNHWVYRVPDDLPDELVAPVNCALSQVIYGL
                     HKAGVRFGDTVVVQGAGGLGLNAAAVAKEMGADKVIAIDGLPSRLELARQFGADETIN
                     ITELDSPEHRIAAVKDATAGRGADVVCDFVGFPAVIPEGIEMLRNGGTYLEVGTISTG
                     VTATIEPARMVFTSKKLIGIYHYDPWTIPTALDFLQRTKEKYPFDRIVSHKFPLEEID
                     RAFSEAEWLNRANNPTAVTRAVIAW"
BASE COUNT          660 a         1138 c         1038 g          628 t
ORIGIN      
        1 ttcgcccatg aggatcgtac ccgtttgaac cgcgaaaccg cctgccagcc acgatacctc
       61 aaccagttgc ccctgaccgg ttcgttgtct gacccacaat gccgcacaga ttgcaccagc
      121 ggcgaggatg gccgcgccgt agctggagag aggcaccgtg aggtatgtcg gacccgggtg
      181 gctcgaatac tgcgtcgcca tgatgccgtc gagcgccgca accaacgcat cgtccggcgc
      241 ccgttcggcg tgcggaccac agctcccgta cggcggcatc cagcaataaa cgaggcgccg
      301 attgatcgcc gaaagagagg cgtaatcgag gccgagtcgg gcgacatccc ccggtgagcc
      361 gtcatacacg agcacgtcgg ccgctgcgac ggcgtcgagt attgcggcgc gtccctgctc
      421 cgtctcaagg tctgcaaccg ccccctcctt gcttcgattg agcgcgcgga agccaggctg
      481 ggtccggtac ggatcgccct cgggcggctc gattttcagt acgtcggcgc cctgctcggc
      541 cagcagcatc gcgccgtacg gaccggcgat gccctgagcc aggtcgatga ctcggatacc
      601 gtccagtgcg ccggacatgg gttgacctct acaacggcgg tgcggaggat tctgtggttc
      661 tggcgatctc ttcggacccc gcgggcgcgc tcggctgcgt cggcacaaca ggtggtgcag
      721 gcggttgcgt cgtctgtatg aggtcatcgg gccgcgccca tttggccggt tcctcagacg
      781 gtcgcgtcgg cgcaccggct ggggagccat acggcgtgag cattggcgcc gcgccgggtg
      841 cgtaaccgcc catgatcgct ccgggggcct gcctgccttc gctcgcaccg gccggctgcc
      901 agtcggtcag ccgccaaagt ccaacaaaca acaacaccag caggccgact attgagaccg
      961 caaagcgcaa gaagcgcccc gccgtatcga gctccttggg cgacccgccc gaaaacagcg
     1021 ccagcaagct cgacgccggt ggagcggcct ggggaacctg ctgcaatgtg taccaggtgc
     1081 cggccccgac caacagcagt ccgccgaata cgaatcccag gcgcaaatag ttgaccacgc
     1141 gcggcaacgc gacgccgatg acgcccagaa cgatacctat accggcgaac cagagaaaga
     1201 tgttgcgctg gtgatccggg aatttctcgc ggtaggtctg attgtcgctg taaaatccat
     1261 cgatctgctt gttgatgcgg gtgctatcct ggtccgtgct attgccgttc aactgggaga
     1321 aactgaggcc aacgggctgc ggcggacgcg gcccggactc aaacgtcagc acgccaaaac
     1381 ccgcggagac ggccaggaca agccctagca ataccgcaaa actgaccctg atccacgtcg
     1441 tgctcatccc cggctttccc tccgccaacc gcgccgcgcc gttgaacgcg cctgggccca
     1501 gtgtgtctgg cacagtatac ccattctctt tcagagccgc agccgtgccg caacacgccc
     1561 gacctggccc gattttcgct ggcgctacgg atacgcatca atcgatatcc atgccacttc
     1621 ctcttggaac gcggttgagc tacccttgtg ggggatggta atgccacaag tcggggtgca
     1681 tcgagttcag tctgaaacga ttcggttctc tcgtgcgttg tattaaatta gaagccggtc
     1741 gcccagcccg tcctcgcctt ccggcaccac ctcggcaagc agtaagtaat cagggaggaa
     1801 caggtacatg tccttagccc aacgtgtgct caagatcagc actgttgccg ccgcggtcct
     1861 cagtatcgcc gcgatcagtg gcagcgcatc gggggtcctc gcgcagtcca cgccacccgt
     1921 cgcccgcatt ttcggctcaa tcacattcac cggatcaacc aacctgaact gggcaacggt
     1981 tacggcctac agcggcagca ccacatgcgg aacggggtca gctgacggtc ggtacgtcga
     2041 tatcgacagc ggcaaccctg gctgtaacag ggtcggcacg gtgatcacct ttaaggtcgg
     2101 cagccaatgg gctaaccaaa cattcgttat cccggatcta cccggttccg cccagcaggt
     2161 caatctgacc ggtccggccg catcgcagcc gggaggtggt ggcgcggcca cttctagcgc
     2221 aacgtactcg ccgggctgga acctcgctgg tggccctgcg ggcaccttat tctcgcaggc
     2281 agctggggtc ctctatacgt tcccggcggg cgcgagcgcc tacacgcaag tgccgaacac
     2341 ccagccagcg caggccggtg tcggctactg ggcgttcttc aacagccaaa ccaccgttac
     2401 cctcagcggc agcacgtccc tgccgttcac cgttaccgcg cccgcgggcc agtacatcat
     2461 gatcgccaat cccagcgcga ccagcaccgt gactgtcagc ggcgccgatg tggtctatac
     2521 ttacgacccg accaacgccg ccagcccgta ctcggcagtc tccacattgc ggccgggaca
     2581 gggtgcatgg gtctattccg ccgccggcgg aacgatcacg atgcagtaac attctgcggg
     2641 accatggacc gatcatcccg gaatgcagga aaggggaggc ggatccgcct cccctttccg
     2701 tttccctgga aaacgcaact gcgtactgtc gtttaccagg cgatgacggc gcgcgtcact
     2761 gccgtgggat tgttggcgcg gttcagccac tcggcctcgg agaaggcgcg gtcgatctct
     2821 tctagcggga atttgtgcga cacaatccga tcaaacgggt atttctcttt ggtgcgttgc
     2881 agaaaatcga gcgccgtcgg gatcgtccac ggatcgtagt gatagatgcc gatcagcttc
     2941 ttgctggtga acaccatccg tgcaggctcg atggtagcgg taacgcctgt gctgatggtc
     3001 ccgacctcca ggtaggtgcc gccattacgc agcatctcta tcccttccgg gatcacggcc
     3061 ggaaatccga cgaagtcgca gacgacgtcc gcaccccgcc cagcggtcgc gtctttgacc
     3121 gccgcgatac ggtgttccgg cgaatcgagc tcggtgatgt taatcgtttc gtcggcgccg
     3181 aactggcgcg caagctccag tctggaaggg agaccgtcga tcgcgatgac tttgtctgcc
     3241 cccatctcct tggccacggc ggcggcattt aatcccaacc cgcctgcgcc ctggacgacc
     3301 acagtgtcgc cgaagcgcac gcccgcctta tgcagtccgt agatcacctg tgatagggcg
     3361 caattaaccg gcgcgaccag ctcatccggc aggtcgtcgg gcacgcgata tacccagtga
     3421 ttaggacgca aatagtagaa gtcggcgaag gcgccggtga agta
//
LOCUS       DATCPE010000034         8361 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U7219
            k141_395100, whole genome shotgun sequence.
ACCESSION   DATCPE010000034 DATCPE010000000
VERSION     DATCPE010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955033
            Sequence Read Archive: SRR1586279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 8361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 16:49:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 640
            CDSs (total)                      :: 631
            Genes (coding)                    :: 627
            CDSs (with protein)               :: 627
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8361
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_395100"
                     /isolate="SMAG_U7219"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1133..2143
                     /locus_tag="VIM45_01080"
     CDS             1133..2143
                     /locus_tag="VIM45_01080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8819911.1"
                     /translation="MSFRQTLGPHATGNSEAYVAPFSSPPSPQISPAPGMGTVPTEGE
                     IRSASTAVLTTEGLEPLKELVAKAQSERAVLLPELHQAKSAATKATKRCEDWTNGWLL
                     RRVFKNRFAHIAAIAEEASAKAAELAEQERLSRLATQFDLPAQLKDAFGRVCDATAFL
                     AQSRRIWDTLSRVATDRVRERSTATSSINRELVTVGLGSSDVIESQFDVPHLSNANGG
                     DVFIYPGFVLYHISASAFALVDAREVTITSSAAPFIEEEGVPTDSEIVAQTWKKANKD
                     GSPDKRFANNYQIPIVRYGRLRLESKTGLNEEYMVSNFASSERFAAEWAGFQKALSAA
                     TT"
     gene            2276..2707
                     /locus_tag="VIM45_01085"
     CDS             2276..2707
                     /locus_tag="VIM45_01085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8819912.1"
                     /translation="MGIARSFPIALAVLSVACHSSPIGPGAGRVVGIIDAGQSFPQVI
                     DAPAQASLGQSFSVTVSTIGNSCVRPAGADVTIDGLVATITPYDVEFVTGVTCLEIPK
                     ASPHVLQLSFGQAGTATIRVKGRSDYQPGLVTVERSLIVSQ"
     gene            complement(2778..2987)
                     /locus_tag="VIM45_01090"
     CDS             complement(2778..2987)
                     /locus_tag="VIM45_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8819913.1"
                     /translation="MFVRPVKRRRDKARHVSQAGLGGTREEIEQTRLSINASVSMKTD
                     DGVWSIYFNTVLIATLNERDYIIRG"
     gene            3199..3465
                     /locus_tag="VIM45_01095"
     CDS             3199..3465
                     /locus_tag="VIM45_01095"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY8819914.1"
                     /translation="MTNETAPPPLLAQSLEASLTVADVRRSVEWYRDVLRFSVGREFE
                     RGGRLLAVSLRAGSIRILLTQDDGARGVDRVKGEGATRRLTNAE"
     gene            3680..4528
                     /locus_tag="VIM45_01100"
     CDS             3680..4528
                     /locus_tag="VIM45_01100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8819915.1"
                     /translation="MVSGTGSASEIYAMRPDGSARLQLTQNAILDGDPDWSPDGQQIV
                     FTSAVDSEPGAPARRRDVYLMNADGSGKHRLFQGAPGAGAAHPRWSPDGSRISFDSFD
                     AALGGFQPYVMNSDGSNVKIVRTMPGENFGLAWSPDGTRFLFLTNRSPRLLWTLYVMP
                     TDGSSDQQITDDAACPSNLGRAAQWSPDGSSIAFSCATNFGSWIGTMRADGTALVVVT
                     VPATEAFDPVWSPSGAQIAFAGNPGSVGGNPVSQVFVINAAGGAVSQITNGTAGNVVT
                     AWRSGH"
     gene            4625..6928
                     /locus_tag="VIM45_01105"
     CDS             4625..6928
                     /locus_tag="VIM45_01105"
                     /inference="COORDINATES: protein motif:HMM:NF036578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-rhamnosidase C-terminal
                     domain-containing protein"
                     /protein_id="HEY8819916.1"
                     /translation="MFDGASPASWIAPSGVPGDSFTVFHARRTFVLATVPTRFVVHVS
                     ADNRYRLYVNGLQVSSGPQRSDVTHWRYETLDLAPHLRAGRNVIAALVWNWGAARPVA
                     QHSNRTGFLLQGNGQVEAALVNTGTGWKLLRDPAYSPIVITTATLGDYYAAAPGDSID
                     GSRYPWGWERADYADGAWPEAAFVDRIKRRAMAPGGYGEVSGWQLEARSIPPMEETEQ
                     RLARVRRTTGVETDGAFLRGAGDLVIPARTTAVLLLDQLHTTNAYPVLETSGGAGSMV
                     RLTYAEALIDAKGQKGNRNEIEGRTVRGVRDVFLPDGGARRRFTTLYWRSFRYIQLDV
                     TTADEPLRVHDLHGIFTAYPFTERARFASDLPWLADMWRMNWNGARIGAFETYMDTPY
                     WEQLQYIGDTRVQGLISLYVAGDDRLLRQAIEHFDLSRIPEGLTTSRYPSALTQIIPP
                     FSLIYVAMVHDYFMHRDDPAFVRERLAGVRGILDWFGRHVDATGMLGPMPYWNYVDWT
                     KPWDRGVPPGADDGHSTTISLLYVYALQRAAELEEGVGMRGAGADYRARAEAVRAAVR
                     ARAWDSARGLFRDAPDISAYSQQTNVLAVLANAVSSAEQRGLMERVLADTTLTPASYY
                     FSFYVLEALRKAGLGDRYIEQLAPWQAMLKLGLTSAPENPEPTRSDTHAWAAHPNYGL
                     LATVLGVRPSSPGFRTVRIAPALGPLRRAEGRVPHPLGDIDVQLVRERQRGLRAEITL
                     PRGLSGVLEWQGQSRPLREGRQIIRFE"
     gene            7015..7437
                     /locus_tag="VIM45_01110"
     CDS             7015..7437
                     /locus_tag="VIM45_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005269216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEY8819917.1"
                     /translation="MLFFRSSSPRQLSLGLGALRVAVATIFIKHGAQKLFVFGFSGVT
                     GAFTQMGVPLPGVTGPFIALLEFFGGIALVIGLLTRLIALGLVCDMLGAILLVQLKSG
                     FSHYELEFLLLGSSLALVLAGPGGFSVDALLGRRDAKD"
     gene            7444..>8361
                     /locus_tag="VIM45_01115"
     CDS             7444..>8361
                     /locus_tag="VIM45_01115"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY8819918.1"
                     /translation="MRNPSEEESSLRKLDGAIRQPRAGKLLTAPLLPIMIAVFTVALA
                     VFQEPADVPPWAFPGSPKDSGVVDSTRLNRVPNSRARFTLLQAIDRFAPPEWHPSGHP
                     PMPDVVAHGRKPDLFACAYCHLPNGAGRPENVALAGLPEGYIIAQVADFRSSARRNPL
                     SGWIPGNSMHTVAMSATEADVASAARYFSRLPFKPQVRVIEAARIPRMREAAFVYARI
                     PGKGTEPIGRRIVEGPPDFERHELRDDAMIYVAYVPPGSIKRGRSIATKGTDGLATAC
                     ITCHGKNLLGIGLVPRLAGRYPTYLVRQLL"
BASE COUNT         1582 a         2523 c         2551 g         1705 t
ORIGIN      
        1 ccaggctaac gaacgctgaa gcagacgagc gatcttcggt agctcgctac gctcgcattt
       61 tgttagactc gctcgcagct tagctaaggc gttaggttgc aattccagat tagcgcctca
      121 tgaacactcg cccgaaaaca aagattcaag ccgagcatgc ggtcgggcgg aagcttcgac
      181 catttctcgc tgaatatctt cgtgcaattc aaaaaacaga tcggctcatt tatcaactct
      241 tagcctcacg cgagaacaaa ccgagcaatg atcgggctcc cgtggtctgc gcaatactgt
      301 tagctcgtct cagtaatgat ctgcgagcat gcgggctgct tgcgagccgg ggccacatcc
      361 tccagtcatg gacaatcggc gcctcggccc tcgagagtgc atacggcgtc ggattcgtcg
      421 gaagaagcga ggagcgagca aagaggtggt ttgatcataa ggatttggcg aaagctgcct
      481 ggccagcctt tgacgcattg gcggccactc ttcggacgtt cgatctttcg agcaacctag
      541 aaaacttttt cgggtcgtac cgacgtcttt ccgccgcgaa acacggaaac ccgttgattc
      601 aaagccgata cggcgtgtcg aaatcaaagc tcgcgacacg aatccaagtt gatccgcact
      661 tcggcagggg cgccgctaga gcagcgcgct acgggatagc atggacgctt cacggggcgg
      721 gcttggggat gtggtcttac gcacactatt tccctaccgg taaacccctt gagcaagaaa
      781 tcgctcaatt cgcccgggta acgctcgcct tagcggcgag ggctcgtgaa gtctagcacg
      841 gcgcgtttgc accctaacga acgttgcagc tgacaagcac ttttcggatg cggcttcgcc
      901 gcaatggttg taacgtgctt gcagctgaac ttgggcgtta ggtggctggc agacacattt
      961 ccttggaccg aatgagatga gttggcggtt cagaaagagt tttagtcccc ttccaggcgt
     1021 tcggctaaac ttttccccac gcggtattag cacgtcggtg ggcgtcggac cagttcgact
     1081 gtacctcggt cctaaaggcg cagcggtcac cacgcgcatt cctggatccg gtatgtcctt
     1141 tcgacaaacc ttgggccccc atgcaacggg caattctgag gcatacgttg ctccgttcag
     1201 ctcaccgcca tcacctcaaa tctcgcctgc gcccggaatg ggaaccgttc cgacagaagg
     1261 cgagattcgc agcgcaagca ctgcggttct aacaaccgaa ggattagaac cgctcaagga
     1321 actagttgcg aaggcgcaaa gtgagcgagc cgtacttctt cctgaacttc accaagccaa
     1381 gtccgccgcc acgaaggcga ctaagcgttg tgaggattgg acgaatggtt ggttgcttcg
     1441 tcgcgtattc aagaatcgtt tcgcccatat cgcggccatc gcagaggaag catcggcaaa
     1501 ggccgctgaa ctcgcggaac aggaacgtct ttcacgcctc gctacccagt tcgacctgcc
     1561 tgcacaactg aaggatgcct tcggccgcgt gtgcgatgct actgcattcc tcgcgcaatc
     1621 acgtcgaatt tgggacacgc tgtcacgagt agcgacggac cgagttcggg aacggtccac
     1681 ggcgaccagc tcgattaacc gcgaacttgt aactgttggt ttagggtcga gcgacgtaat
     1741 cgaatctcag ttcgacgtgc cccatcttag caacgccaat ggtggagatg tttttatcta
     1801 tccgggtttc gttctgtacc acatctcggc atctgcattt gcgctggtcg atgcgcgaga
     1861 ggtgacgatc acatcttcgg ctgctccatt catcgaagag gaaggcgttc caactgactc
     1921 cgagattgtc gcgcaaactt ggaagaaggc taacaaggat ggttcacccg acaagcgatt
     1981 tgcgaacaat taccagatcc cgatcgttag gtacgggagg ctgcgacttg aaagcaaaac
     2041 tgggctgaac gaggaataca tggttagcaa cttcgcatct tcagaacgct ttgccgcgga
     2101 atgggctggc ttccagaaag cactgtccgc ggccaccacc taacgaatgt tgcagcagac
     2161 gggcgcggcc aagtcgttgg gttgtgtatg ttcagagtag aatcgcccgc agctgaactt
     2221 aggcgttagc tagacaacca tcctgcacgt aacttagagc gtatcggagg gttacatggg
     2281 cattgctcgt tcatttccaa tcgcactggc cgttctatca gtcgcctgcc actcgtcacc
     2341 aatcgggccc ggagccggac gagtagttgg gattatcgac gccggccaat cgttcccaca
     2401 ggtcatagac gctccggccc aggcttcact cggtcagtcc ttcagcgtta cggtctccac
     2461 aattggcaat agttgtgtaa ggcctgctgg agctgacgtg acgatcgatg gacttgttgc
     2521 cactattact ccctacgatg tcgaattcgt gaccggcgtt acgtgtcttg aaattccgaa
     2581 agcgtctcct cacgtgctgc agctcagttt cgggcaggca ggtacagcaa ccatacgagt
     2641 gaagggcagg agcgactacc agcccggact ggtaacagtg gagcgctctt tgatcgtaag
     2701 ccagtaaact aggtcgtcga gtgagcagcc gggatcatag gtaacaagag tgtccagcaa
     2761 catgggtaac acttttgtta tcctcggatg atgtaatcac gttcattcag cgtggctata
     2821 agcacggtgt tgaaatagat ggaccagact ccatcgtcgg ttttcatcga gaccgatgcg
     2881 ttgatcgaca agcgcgtctg ctcgatctcc tcacgcgtgc caccgagtcc agcctgcgac
     2941 acgtgccggg ctttgtcgcg gcggcgcttc accggtctaa cgaacaacca ccatctttcc
     3001 ttctcgtcac tatgcgcatc gcattgaacg tctcaggcgt cttgtgtctg ctcgtgggat
     3061 gtgtctggat tcttcaggga atcaacatcc ttccgggtag cttcatgact ggccaaacga
     3121 agtgggccat ctacggcggc cttctagtgg tcgcaggcat cggtctgttg atctcggcaa
     3181 atcgtcgcag agtctgaatt gaccaacgag actgccccgc ctccgctact cgcgcaaagc
     3241 ctcgaggcgt ccctcacagt ggccgacgtt cgacgcagcg ttgagtggta tcgcgacgtg
     3301 ctccgcttct cggttggccg tgaatttgaa cgcggtggcc ggctgctcgc cgtcagcctg
     3361 cgggcgggat cgattcgcat cctcttgacg caggacgatg gggcccgggg agtcgatcgc
     3421 gtgaagggag aaggagccac gcgacgcctg acgaacgcgg aataactcgg cgatcagtca
     3481 ttgcctttag cttgactggg accgttgcgg cccaacagat tcagcgcaca ggattctgaa
     3541 gatccgtcga gaggctccgt cgccacctat gagttgtatc cgcgctgcac cggccctcgg
     3601 tgcctggctg gcaatctgcc tcgccgcatg cggcggggat tctacttcac cgctgaatga
     3661 tccccgtgga gtcatgctgg tggtgagcgg gactggttcc gcgtccgaga tctacgccat
     3721 gcgtcccgat ggatccgctc ggctgcagct cacccaaaat gcgatcctgg atggtgatcc
     3781 ggattggtca cccgacggtc agcagatcgt gttcaccagt gccgtggaca gcgagccagg
     3841 tgcgcccgct cgccggcgcg atgtctatct gatgaacgcc gacggctccg gaaagcatcg
     3901 attgtttcag ggcgcccccg gggccggggc tgcacatccc cgctggtcac cggacggaag
     3961 ccggatttcc ttcgacagct tcgacgcagc tttgggtgga ttccagcctt acgtgatgaa
     4021 cagtgacggt tccaacgtga agatcgtgcg gacaatgccg ggagaaaact tcggtctagc
     4081 gtggtcgccc gacggcacgc gctttctctt tcttacgaac cgatcaccga ggttgctgtg
     4141 gacgttgtac gtcatgccaa cggacggttc cagcgaccag cagatcacag acgacgccgc
     4201 ctgcccgtcg aatctcggga gggcggccca gtggtcgcca gatggctcca gcatcgcttt
     4261 ttcgtgcgct actaactttg gatcgtggat cggtaccatg cgggccgatg ggacggcttt
     4321 ggttgtcgtg accgtccccg cgacagaagc tttcgatccg gtgtggtcac caagtggcgc
     4381 gcaaatcgcc ttcgccggca atcccggttc ggttggcggt aatcctgtct cgcaggtgtt
     4441 cgtgatcaac gccgcgggag gggcggtgtc gcagataacg aatggcaccg caggtaatgt
     4501 cgtgaccgcc tggcgatcgg ggcactagcc cataggcact cttcgtctgt gcttcgcctc
     4561 gatcctcgcg gcgttcgcgg ccatcgtcgc gttctccgga ccgctcgacg cgcaggagac
     4621 gcgcgtgttc gacggggcgt cgccagcgag ctggatcgcg ccctccggag tgccgggcga
     4681 cagtttcacc gtgttccacg cgcgacgaac cttcgtgctg gcgacggtgc ccacgcgatt
     4741 cgtggtgcac gtgtccgcgg acaatcgcta ccgactctac gtcaacggcc tgcaggtatc
     4801 ctctggccca cagcgctcgg acgtcacgca ctggcgctac gagactctcg acctcgcgcc
     4861 ccatctgcgc gcggggcgta acgtgattgc ggccctcgtg tggaactggg gcgctgcgcg
     4921 tccggtggcg cagcacagca atcgcaccgg attcctgctc cagggtaacg gccaggtcga
     4981 ggcagcgctg gtgaacacag gcacaggatg gaagttgctg cgcgatccgg cgtactcgcc
     5041 catcgtcatc accactgcta cgctgggcga ctattacgcc gcggctcccg gcgattcgat
     5101 cgacggctcg cgctatccat ggggatggga gcgcgcggac tacgcggacg gtgcgtggcc
     5161 ggaggctgcg ttcgttgatc gcataaagag gcgcgcgatg gcgcccggcg gctatggcga
     5221 agtgtcggga tggcagctcg aggcgcggtc gataccccca atggaggaaa cagagcaacg
     5281 gctcgcgcgc gtccgtcgca cgaccggtgt cgaaaccgac ggcgcgtttc tacgaggagc
     5341 cggtgatctg gtgatccccg cacggacgac ggcggtgctc ctgctcgatc aattgcatac
     5401 gacgaacgcg tatccggtgc tcgagacgag cggcggggca gggagcatgg tgcgactcac
     5461 atacgccgag gcgctgatcg acgcgaaggg acagaaagga aatcgcaacg agatcgaggg
     5521 gcggacggtg cgcggcgtgc gcgacgtctt ccttcccgac ggcggcgcgc ggcgccgctt
     5581 cacgacgtta tactggcgct cgttccgcta catccagctg gacgtcacga cggcggacga
     5641 gccactgcga gtgcacgatc tgcacgggat cttcactgcc tacccgttca cggagcgtgc
     5701 ccgcttcgcg agcgatcttc cgtggcttgc cgacatgtgg cgcatgaact ggaacggcgc
     5761 gcgaatcggt gcgttcgaga cgtacatgga cacgccgtat tgggagcagc tccagtacat
     5821 cggcgacacg cgcgtgcagg ggctcatctc gctctacgtc gccggcgacg atcgtctcct
     5881 gcgtcaggcc atcgagcatt tcgatctgtc gcgcattccg gaagggctta cgacaagccg
     5941 ttatccctcg gcgttgacgc agatcattcc tcccttctcg ctcatatatg tcgcaatggt
     6001 gcacgattat ttcatgcacc gggatgaccc cgcgttcgtg cgggagcgtc tcgccggcgt
     6061 gcgcgggata ctggactggt tcggccggca cgttgacgcc acgggcatgc tggggccgat
     6121 gccatactgg aactacgtcg attggacgaa gccgtgggat cggggcgttc ctcccggcgc
     6181 cgacgatggg cactcgacga cgatcagcct gctgtacgtg tacgcgttgc agcgcgcggc
     6241 ggagctcgag gagggagtcg ggatgcgcgg tgcgggtgcc gactatcgtg cgcgcgctga
     6301 agcggtgcgt gcggcggtac gcgcgcgcgc ctgggactcc gcgcggggat tgtttcgcga
     6361 cgcgccggat atcagtgcgt acagccagca gaccaacgtg ctcgccgtcc tggcgaatgc
     6421 ggtgtccagc gctgagcagc gaggactcat ggagcgcgtc ctcgccgaca cgacgctcac
     6481 gccggcgtcc tattacttca gcttctacgt gctggaagcg ttacgaaagg cgggactcgg
     6541 cgaccgctac atcgaacagc ttgcgccgtg gcaggcgatg ttgaagctcg gtcttacatc
     6601 ggctccggaa aatccggagc ccacgcgctc cgacacccat gcgtgggccg cgcacccgaa
     6661 ctatggcctg ctcgcgactg tgctcggcgt gcgcccatcg agtccggggt ttcgcaccgt
     6721 gcgcatcgcg ccggcgctcg gtcctttgcg tcgcgctgag ggacgcgtgc cacatccgct
     6781 cggcgacatc gacgtccaac tggtgcgcga gcgccagcgc ggcctgcgcg ccgagatcac
     6841 gctgccgcgc gggctatcag gcgtactcga atggcagggg cagagccggc cactgcgcga
     6901 ggggcggcag atcattcggt tcgaataagc cgacgtgacc ggtaccaagt actaccgaat
     6961 tctgcctcgt cctaaccggg atagattcct gacagcctaa caaaggatca gtcgatgtta
     7021 ttctttcgct cttcttcccc tcgccagctg agcctcgggc ttggggcact ccgtgtcgcc
     7081 gttgcaacga tcttcatcaa acacggggcg cagaagttgt tcgtctttgg tttctccggc
     7141 gttacgggcg cgttcacgca gatgggcgtc ccccttccgg gcgtcacggg accgttcatc
     7201 gcgctgctcg agttcttcgg cggcattgct ctcgtaatag gtctcctgac ccgtctgatc
     7261 gcgctcggct tagtgtgcga catgctcggt gccatcctct tagtgcagct caagagcggg
     7321 ttttcgcatt atgagctcga attcctgctc ctcgggtcga gcctcgctct tgttcttgcg
     7381 ggacccggcg ggttctccgt cgacgcgctg cttggtcgtc gtgacgcgaa agattgactt
     7441 cgcgtgagga acccaagcga ggaagaaagc agcctccgaa agcttgacgg agcgattcgt
     7501 cagccacgtg ccggcaagtt gctgaccgcg ccgttactgc cgataatgat cgccgtcttt
     7561 acggtcgctc ttgccgtctt ccaagagcca gccgatgtgc cgccatgggc tttccccggc
     7621 agcccaaagg attccggggt cgtcgatagc acgcggttga atcgcgtccc caacagcagg
     7681 gcgcgattca cgctgcttca agccatcgat cggttcgcgc ccccggagtg gcatccgagc
     7741 ggacatccgc cgatgccgga tgtcgttgcg catggccgga agccggatct ctttgcgtgc
     7801 gcctactgtc atctgccgaa cggagcggga agaccagaga acgtagcgct tgccggcttg
     7861 cccgagggct acatcatcgc acaggtggcg gactttcgca gttctgcccg acgaaatccg
     7921 ttgtccggct ggattcccgg caacagcatg cacaccgttg ccatgagtgc aacggaagca
     7981 gacgtcgcct ccgccgcgcg ctacttttcg cggctgccat ttaagccgca agttcgagtg
     8041 atcgaggcgg cgcgcattcc acgcatgcgc gaggccgcat tcgtctacgc gcgcatcccg
     8101 gggaagggaa ccgagccgat cggcaggcgg atcgttgagg gtccgcccga tttcgaacgc
     8161 cacgagctcc gcgacgacgc catgatctat gtcgcctacg taccgccggg gagcatcaaa
     8221 cgtggccgct ctatcgcgac gaaaggcacc gacgggctcg ccaccgcttg cataacgtgt
     8281 cacggcaaaa acctgctcgg cataggcctc gtgccgcggc tcgccggccg ctaccccacg
     8341 tatctcgtcc gccaactcct c
//
LOCUS       DATCPE010000035         2906 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U7219
            k141_589570, whole genome shotgun sequence.
ACCESSION   DATCPE010000035 DATCPE010000000
VERSION     DATCPE010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955033
            Sequence Read Archive: SRR1586279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 16:49:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 640
            CDSs (total)                      :: 631
            Genes (coding)                    :: 627
            CDSs (with protein)               :: 627
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2906
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_589570"
                     /isolate="SMAG_U7219"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1633
                     /gene="dxs"
                     /locus_tag="VIM45_01120"
     CDS             <1..1633
                     /gene="dxs"
                     /locus_tag="VIM45_01120"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEY8819919.1"
                     /translation="GRRERFATIRQHEGLTPFCERDESIHDVWGAGHASTSLSAAFGM
                     ALARDLKGEHHQVVAVIGDGGLTGGMALEAINHIGHLGKRLIVVLNDNGMSIAPNVGA
                     INRIFNKVRLDARYQSAKTTMGQIAKKMPMGELGWEASRRVKRSVKGLLMPSGFFEEL
                     GFDYIGPVDGHNIGELRAALQSAMLIEDRPVFIHAITQKGHGYEPAEIDPVKLHGVSP
                     PGGSGGPPKWQDVFGAAVGELMRQDRYVTAITAAMPDGTGLVPVLKEFPDRSFDVGIC
                     EQHAVTLAAGMATQGLAPIVAIYSTFLQRAYDQIVHDVCVQNLHVVFALDRAGFVGDD
                     GRTHQGAFDLSYLSCLPNMVIAAPKDENELRDLLYTGLEHLGPFAVRYPRGAAAGVEI
                     QPGFRAIAIGSWEVLREGTDVAILALGATIPPALAAAEELAQHGISAQVINARFVKPL
                     DTVMLLDAARTVRARHVLTVEENVMRGGLGSAVAVELQKAGLADVRLDCLGMPDKFVE
                     HGSQNIQRGRYGITANAIVQRIMAPSLSAEPVAAS"
     gene            1684..2715
                     /locus_tag="VIM45_01125"
     CDS             1684..2715
                     /locus_tag="VIM45_01125"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEY8819920.1"
                     /translation="MATAAPPALDRFRDAVVHEMRDTLRTHDQSIYGLQRYHLGWQAS
                     DGVPTERVTGKMFRAALCLLCCEGVGGDSERALPAAAAIEFLHNFSLIHDDIEDVSRS
                     RRGQRTVWDVWGVARAINAGDSMFVVARMALHRLAAHGYQPQTLVNAFLLFDRAGQRM
                     CEGQDLDLSFEDRESVSMDEYQRMIAGKTGALIGASASLGALLGDAPAPTVALFDRFG
                     RLLGRAFQIQDDILGVWGLESRTGKPSGSDIRSRKKAYPVVRALELLSPGQRSRLLGI
                     FAQPELDDAAVEEVLSILDEHGIRTAAETEAVRAATDAIALISQATLVGPVRRELTAL
                     AEFVADRDA"
     gene            2749..>2906
                     /gene="pgk"
                     /locus_tag="VIM45_01130"
     CDS             2749..>2906
                     /gene="pgk"
                     /locus_tag="VIM45_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012615945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEY8819921.1"
                     /translation="MNKKTVRDVDVSRKRALVRVDFNVPTDKSTGEITDDTRIRASLP
                     TINYLIDH"
BASE COUNT          541 a          907 c          924 g          534 t
ORIGIN      
        1 gggccggcgc gaacgctttg caaccattcg tcagcacgaa ggcctgacgc cgttttgcga
       61 gcgcgacgag agcatccacg atgtgtgggg cgcgggacac gccagcacgt cgctgtcggc
      121 agccttcggc atggcgctgg cgcgcgacct gaaaggcgag caccaccagg tggtggccgt
      181 gatcggcgat gggggcctca ccggcggcat ggcgctggag gcgatcaacc atatcggcca
      241 cctgggcaag cgactgatcg tcgttttgaa cgacaacggc atgtcaatcg ccccgaacgt
      301 cggcgcgatc aaccgtattt tcaacaaggt ccgactcgac gcgcggtacc agtccgccaa
      361 gacgaccatg ggccagatcg cgaagaaaat gccgatgggc gagttgggct gggaggcgag
      421 ccggcgcgtc aagcgcagcg ttaaaggtct gctgatgccg agcggctttt tcgaagaact
      481 gggcttcgat tacatcggcc cggtggatgg acacaacatc ggcgaactgc gcgccgcatt
      541 gcaaagcgcg atgctgatcg aagatcggcc ggttttcatc cacgcgatca cgcagaaggg
      601 tcacggctat gaacccgctg aaatcgaccc ggtgaaactg catggagtat cgccgcctgg
      661 gggctccgga ggcccgccca agtggcagga cgtattcggc gccgccgttg gcgagctgat
      721 gcgacaggat cggtatgtca cggcaataac ggccgcgatg cccgacggca ccggactggt
      781 tccggtcctg aaagagttcc ccgaccggtc gttcgatgtc ggcatctgcg agcagcacgc
      841 cgtgaccctc gccgccggta tggctaccca ggggctagcg ccgatcgtgg cgatctattc
      901 caccttcctt cagcgtgcat atgaccagat cgtgcatgac gtctgcgtcc agaatcttca
      961 cgtcgtcttt gcgctcgacc gtgccggctt cgtcggcgac gacgggcgga cccaccaggg
     1021 ggcatttgac ctttcctatc tgtcgtgcct gccgaacatg gtgatcgccg cgcctaagga
     1081 cgagaacgag ctgcgggacc ttctctatac cggattggaa catctgggtc cgtttgccgt
     1141 gcgctatccg cgcggcgcgg cggcaggtgt cgagattcag ccaggctttc gcgcgattgc
     1201 aatcgggagc tgggaagttc ttcgcgaagg tacggatgtc gccatcctgg cgctgggcgc
     1261 tacgattccg ccggcccttg ccgcagccga ggagcttgcg cagcacggga tttcggcaca
     1321 ggtcattaac gcccgcttcg tcaaaccgct cgataccgtg atgctccttg acgctgcgag
     1381 gacggtccgc gcgcggcacg tgctgaccgt ggaagagaat gtcatgcgtg gcgggctggg
     1441 aagcgcggta gccgtggagc tacagaaggc cggtttggcc gacgtgcggc tggactgcct
     1501 ggggatgcca gacaagttcg tcgaacacgg gtcacaaaat atccaacgcg gtcgctacgg
     1561 catcaccgcc aacgccatcg tccagcggat catggcgccg tcgttgtccg ctgaaccggt
     1621 tgccgcctcg taggtttcag ccccgagatt cggcgcatcc ggcccctaca tggagccgca
     1681 gccatggcca cagccgctcc cccagctctc gatcgatttc gagacgcggt cgtgcacgaa
     1741 atgcgtgata cgctgcggac ccacgaccag tcgatctacg gcctacagcg ctaccacctc
     1801 ggatggcagg cgtccgacgg cgtccctacc gagcgcgtga ccggcaagat gttccgggca
     1861 gcgctgtgcc tgctctgctg cgagggcgtc ggcggagact ctgagcgcgc cttgcctgcg
     1921 gccgccgcca tcgagttcct ccacaatttc tctcttatac acgacgatat cgaagacgtc
     1981 agccgaagcc ggcgcggcca gagaacggtt tgggatgtgt ggggggttgc ccgcgctatc
     2041 aatgccggcg attcgatgtt tgtcgtcgcc agaatggctc tccaccggct agccgctcat
     2101 ggttatcaac cccaaacgct cgttaatgca tttctgctct tcgaccgcgc cggtcagcgt
     2161 atgtgcgaag gccaagacct cgacctctcg ttcgaagatc gcgaatcggt gtcgatggat
     2221 gaataccagc ggatgatagc gggcaagact ggggcgctga tcggcgcttc ggcctcgctg
     2281 ggcgccctcc ttggcgatgc cccggcgcct acggtcgcgc tattcgatcg ttttggcaga
     2341 ctgctgggcc gcgcttttca gatccaggac gacatcctcg gcgtctgggg gctggaatcg
     2401 cgcacgggca agccatccgg cagcgacatc cgttctcgca aaaaggcgta tcccgtcgtg
     2461 agggcgctcg aactgctttc gcccggacag agatcgcgct tgctcggcat ctttgcgcaa
     2521 cccgaactag acgatgcggc cgtagaagag gtgctgagca tcctcgacga acacggcatc
     2581 cggaccgctg ccgagaccga ggccgtgcgt gccgctactg acgccattgc gttgatcagt
     2641 caggcaacgc ttgtggggcc ggtccggcga gagttgaccg cgctcgcaga attcgtcgcc
     2701 gatcgcgacg cctgaaggcc ctgcgacccc atccggtata cttgcggcat gaacaaaaag
     2761 acagtccgcg acgtggacgt ttcccgaaag cgagcgctcg tacgggtcga cttcaatgtt
     2821 ccgacggaca aaagcacggg cgaaatcacc gacgatacgc gcatccgcgc gtcgctaccg
     2881 actatcaatt acctgatcga ccatga
//
LOCUS       DATCPE010000036         5528 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U7219
            k141_232926, whole genome shotgun sequence.
ACCESSION   DATCPE010000036 DATCPE010000000
VERSION     DATCPE010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955033
            Sequence Read Archive: SRR1586279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 5528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 16:49:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 640
            CDSs (total)                      :: 631
            Genes (coding)                    :: 627
            CDSs (with protein)               :: 627
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5528
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232926"
                     /isolate="SMAG_U7219"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2207
                     /locus_tag="VIM45_01135"
     CDS             <1..2207
                     /locus_tag="VIM45_01135"
                     /inference="COORDINATES: protein motif:HMM:NF023266.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ERAP1-like C-terminal domain-containing protein"
                     /protein_id="HEY8819922.1"
                     /translation="DANGEEKAIATTQFESTDARRAFPCWDEPAFKAVFSITLLVDPG
                     MTAISNGSVQSETTDQATGKRTVRFSDTMLLSTYLVAFIIGEFEGTEPVDVAGTPVRV
                     WATPGKQQLAGYALAAGSHAVSFFNGYYGINYPGDKLDLIAIPDFAAGAMENLGASTF
                     RENLLLLNPETSTHGELERVVEVEAHEIAHMWFGDLVTMRWWNGLWLKEAFATFMAML
                     AEDDFKPEWEVWTAFGLSRAGAMLVDGLNSTRSIEYTVVKPEDAAGMYDVLTYEKGAA
                     VLRMLEQYLGSETFRRGINLYLVKHSFGNAETSDLWDALEEASNEPVRKLADSWIFQP
                     GYPLVSADYRDGALTLAQQRFLYHREGEASDQLWQVPVAIRIERDGESGTEKVLLGDR
                     EQRVEIPSGFRSITVNAGGNGVFRVRYSPNLLSKLTADLRSSLSPIERFNLVSDSWAN
                     VLAGYTPLSEFLDLTALFRDEDDVNVWAIILGGFSQARRIIDDANLPSLQALVRDRLQ
                     RMYKKIGWDPKESESDLTKQLRGQIVGALATQGNDPEVQQKARDHFARYKEDPTSVDA
                     NVVAACIGGVAYTGGPEEYADFWQRFKNAATPQEERRFLFSLPGFRDPALLQESLDRA
                     FAGEVRTQDAPSLISQTFYNRAGGELTWRFVTQHWDELLQKFPDNTIMPMVEGITALT
                     RPEQAEETQAFFADHKVPDAGKRLDQALERQRIGVAFRERESTRLVEYLRAP"
     gene            complement(2394..2684)
                     /locus_tag="VIM45_01140"
     CDS             complement(2394..2684)
                     /locus_tag="VIM45_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HEY8819923.1"
                     /translation="MHIGVCHITLRLPENHSLKGKRQVVKSIVARLQNQFNVSAAEVD
                     DNDVWQIAGIGVCCISNDSRHADQMLASVIEWIERNRPDVELVDYETEIITV"
     gene            complement(2746..5022)
                     /locus_tag="VIM45_01145"
     CDS             complement(2746..5022)
                     /locus_tag="VIM45_01145"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013075547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEY8819924.1"
                     /translation="MPEYVETEINGRPLWIETGTLAAQAGGAVTVRYGDTVVLVTATA
                     SPGPRPLDFFPLTVDYEERLYAAGKIPGSFFRREGRPGTEAILAARLTDRPIRPLFPM
                     GFRNDVQIIITVLSADQENDPDVLGTIGASCALMISPIPFEGPVSSVRVGRVGGKFVA
                     MPTFTELQSSDLDLIVAGTREAIMMVECGSQELPEDVMLEAIRFGDQVNKQIIAIQDE
                     LIAKTAKPKMEITPKTVDPGVDQSVRAFLQGKMEAVVDTRVKKNALDAEEQVRAELQE
                     RFGETFQMEQVLAVFESVHEAEVRNSIVNEGRRPDGRGMKDIRPISVAVGVLPRTHGT
                     GLFQRGETQVLTIATLGGVGEAQKIDSLSREVTKRYMHHYNFPPFSVGEVRRVGSPGR
                     REIGHGALAERALEPVVPPVEEFPYTLRLVSETLSSNGSSAMASVCGSTLAMMDAGVP
                     LKKPVAGVAMGLVMSDGKYQVLTDIAGVEDHLGDMDFKVAGTKDGITALQMDIKVKGI
                     TTEIMGEALAQAKEGRLFILEKMLEVLPAPRQEMSPYAPRVYTIHINPEKIGAVIGPG
                     GKTIRSIIEATKATIDIQDDGSVFIGSANEEGARKAIQMVEGLTKEVEIGQVYTGKVV
                     RIMPFGAFVEILPNKDGMVHISELTDHRIDRVEDEVKLGDEVSVVVTEIDHMGRINLS
                     RRALMEVGSRDNGASPDGNGDGGFGAQRGGSLMAGRGGGRPGGGGRPGGGRPMGRGPG
                     GPGGPGGPRRGGPGGPRP"
     gene            complement(5218..5484)
                     /gene="rpsO"
                     /locus_tag="VIM45_01150"
     CDS             complement(5218..5484)
                     /gene="rpsO"
                     /locus_tag="VIM45_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012864242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEY8819925.1"
                     /translation="MLTKEKRDSVVESYRTHEKDTGSSEVQIALLTERILQLTDHMRT
                     HKQDFHSRRGLLKLVGQRRRQLAYLNQTDVERYRGVIARLGLRK"
BASE COUNT         1005 a         1737 c         1693 g         1093 t
ORIGIN      
        1 gcgacgcgaa cggcgaggaa aaggcgattg ccaccacgca gttcgagtcg accgatgcgc
       61 ggcgcgcctt tccttgctgg gacgagcccg cgttcaaagc cgttttctcg atcaccttat
      121 tggtcgatcc gggcatgacc gcgatatcaa acggctcggt acagagcgaa accacggatc
      181 aggcaacggg aaagcggacg gtcaggttca gcgacacaat gctgctgtcg acctacctgg
      241 tcgccttcat catcggcgag tttgagggta ccgagcccgt cgacgtggcc gggacgcccg
      301 tgcgcgtttg ggcgacaccc ggcaaacagc aactggccgg ctatgcgctg gcggcgggca
      361 gccacgccgt ttcgtttttc aacggctatt acggtatcaa ctacccaggt gacaagttgg
      421 acctgatcgc cattcccgac ttcgcagcgg gcgcgatgga aaacctcggg gcctccacat
      481 ttcgcgagaa cctcttgctg ctgaatccag agacctccac ccacggcgag ttggagcggg
      541 tcgtggaggt cgaggcgcac gaaatcgcgc acatgtggtt cggtgacctg gtaacgatgc
      601 gctggtggaa cggcctctgg ttgaaagagg cgttcgcaac ctttatggcc atgcttgcag
      661 aggacgattt caagcccgag tgggaagttt ggacggcgtt cgggctctcg cgagcgggag
      721 cgatgctcgt cgacggcctc aacagcacgc gctcaatcga gtacaccgtg gtcaagccgg
      781 aagacgccgc cggcatgtac gacgtgctga cgtacgaaaa gggcgccgcg gtgctgcgca
      841 tgctggagca gtaccttggc tcggagacct tccgcagggg gatcaacctc tacctggtga
      901 agcattcgtt cggaaacgcc gaaaccagcg acctgtggga cgcgctcgaa gaagcatcca
      961 acgagccggt tcggaagctc gccgattcgt ggatctttca gcccggttac ccgctcgtga
     1021 gcgccgatta tcgcgacggc gcgctaacgc tggcgcagca acgtttcctg tatcaccgcg
     1081 aaggcgaggc ttccgaccaa ctctggcagg tgccggtagc gattcgaatc gaacgcgatg
     1141 gtgaatccgg cacggagaag gttctcctcg gcgatcgcga gcagcgcgtt gaaattccgt
     1201 ccggtttccg ctcgatcacc gtgaacgccg gtgggaacgg cgttttccgg gttcgctatt
     1261 cgccaaactt actctcgaag ctgaccgccg atcttcgcag cagcctctcg ccaatcgagc
     1321 gattcaacct ggtaagtgac agttgggcga acgtcctggc aggctatacg ccgctcagcg
     1381 aatttctcga cctcacggcg ctgtttcgtg atgaggacga cgtgaacgtt tgggcgatca
     1441 tcctcggggg ttttagccag gcgcgccgca tcatcgacga cgcgaacctg ccatcgctgc
     1501 aagcgctggt gcgcgaccgg ttgcagcgga tgtataagaa gatcggctgg gatccgaagg
     1561 agagcgaaag cgacctcacg aagcagctac gcgggcagat cgtcggcgca ctcgccaccc
     1621 agggcaacga ccccgaggtc cagcagaagg cgcgcgatca cttcgcgcgc tacaaagagg
     1681 acccgacttc ggtagacgcc aatgtggttg ccgcctgcat cggcggcgtg gcatataccg
     1741 gcggtccgga agaatacgcc gacttctggc agcgtttcaa gaacgccgcc acgccgcagg
     1801 aagagcgtcg cttcctcttc tcactgcccg gattccgcga cccggcacta ttacaagagt
     1861 cactggaccg cgcgttcgct ggggaggtcc gcacccagga tgcgccttcc ctcatcagcc
     1921 agaccttcta caatcgggcc ggcggcgagc ttacctggcg cttcgtgacg caacattggg
     1981 acgaactgct gcaaaagttt ccggacaata cgattatgcc gatggtcgaa ggcataactg
     2041 ccctcaccag acccgagcag gcggaagaga cccaagcgtt tttcgccgat cacaaagtcc
     2101 ccgacgcggg gaagcggctg gaccaggcgc tcgagcgcca gcggattggc gtcgcattcc
     2161 gcgagcgcga aagcacaagg ttagtagagt acttgcgggc cccctaaccc ccatcccctt
     2221 gcccccaata ctggggggaa ggggcgatcc ctgggtgcac ggctcggccg gcgtcgggca
     2281 tcccgtgtgt cctgtacgtc caaactcaat tgctaacccc cgtgttagct ccctcttgcc
     2341 cccagaattg ggggaaagag ggccggggag aaagggggcc gtcccgactg ccctcacaca
     2401 gtgatgatct cagtctcgta gtccacgagc tccacgtcgg gacggttgcg ctcgatccac
     2461 tcgatgaccg acgccagcat ctggtcagcg tgacgggagt cgttggagat gcagcagacg
     2521 ccgatgccgg cgatttgcca gacgtcattg tcgtcaacct cggcagcgga aacgttgaac
     2581 tggttctgca accgggcaac gatcgacttc accacctgcc gcttgccttt cagcgagtgg
     2641 ttttcgggca aacgcagagt aatgtgacag acgccgatat gcatcgatct tcaaaatggg
     2701 gccggggaga ttccccggcc ccatcagcta cagcctcttt actggttacg ggcgaggtcc
     2761 gcctggaccg ccgcgccttg gaccgccggg ccctcccggc ccgcccgggc cacgacccat
     2821 cggccggccg ccgccgggtc ttccgcctcc gcccggccgg ccaccgccgc ggccagccat
     2881 gagcgatcca ccacgctggg cgccgaagcc accatcgccg ttgccatcag gcgaagcgcc
     2941 gttgtcgcgg ctgccgacct ccatcaacgc acggcgagag agattgatgc gacccatgtg
     3001 gtcgatctcg gttacgacta ccgaaacctc gtcgccaagc ttcacttcat cctcaacccg
     3061 gtcgatgcga tggtcggtga gctcggagat gtgtaccatc ccatctttat tcggcaggat
     3121 ctcgacaaag gcgccaaacg gcatgatgcg cacgactttg ccggtgtaaa cctggccgat
     3181 ttcgacttcc ttggtgagcc cttcgaccat ctggatcgcc ttgcgggcgc cctcctcgtt
     3241 ggccgatcca atgaaaacac tgccgtcgtc ctggatgtcg atcgtggctt tggttgcttc
     3301 gatgatgctg cggatcgtct tgccaccggg tccgatgacc gcgccgatct tctccgggtt
     3361 gatatggatc gtgtacacgc gcggcgcgta tggggacatc tcctgccgcg gcgcgggcaa
     3421 tacttccagc atcttttcga gaatgaaaag ccggccttct ttggcctgtg ccagcgcctc
     3481 tcccatgatc tccgttgtaa tacctttaac cttgatgtcc atttgcaggg cggtgatgcc
     3541 gtccttcgtg cccgcgactt tgaagtccat gtctccaagg tgatcttcga ccccggcgat
     3601 gtccgtgagc acctggtact taccgtcgga catcacgagt cccatggcaa cgccggccac
     3661 aggcttcttg agtggaacgc cggcgtccat catcgccagc gtgctaccgc acacgctcgc
     3721 catcgcgctg ctgccgttgg aagagagcgt ctccgacacg agcctcagcg tatacgggaa
     3781 ctcctccacg ggcgggacaa ccggctcgag cgctcgttct gccagggcgc catgtccaat
     3841 ttcgcggcgg cctggcgagc cgacgcgccg cacttcgccc accgagaagg gggggaagtt
     3901 gtaatggtgc atgtatcgct tggtcacttc ccgtgagaga ctgtcgatct tctgcgcctc
     3961 gccgacgccg cccagcgtgg cgatcgtcaa gacctgtgtc tcgccgcgtt ggaacagacc
     4021 ggtgccgtgc gtgcgcggca ggacgccaac ggcgacactg attggccgga tgtctttcat
     4081 accccgacca tcggggcggc gtccctcgtt cacgatcgaa ttgcgcactt cggcctcgtg
     4141 tacgctttcg aagaccgcca ggacctgttc catctggaac gtctcgccga accgctcctg
     4201 cagctcagcg cgcacctgct cctcagcatc cagcgcgttc ttctttacac gggtatcgac
     4261 gacggcttcc atcttgcctt gaaggaaagc ccgcaccgac tgatcgaccc cagggtcgac
     4321 cgtcttcgga gtgatctcca tcttgggctt tgctgtcttg gcgatcagct catcctggat
     4381 ggcgatgatc tgtttgttga cctggtcgcc aaagcggatc gcttcgagca tcacgtcttc
     4441 gggcaactct tgcgagccgc attcgaccat catgatcgct tcgcgtgtcc ccgccacgat
     4501 caggtcaagg tcgctggact gcagttcggt aaaggtcggc atcgcgacga acttgccgcc
     4561 gacgcgcccc acgcgcacgc tgctcaccgg tccctcgaag gggatcggag agatcatgag
     4621 cgcgcacgag gcgccgattg tgcccagcac gtccggatcg ttttcctgat ccgccgacag
     4681 caccgtgatg atgatctgaa cgtcgttgcg gaatcccatg ggaaagagcg gtcggatggg
     4741 gcggtccgtg agccgggcgg ccaagatcgc ttccgttccc ggcctgcctt cacggcggaa
     4801 aaagctgccg ggaatcttgc ctgccgcgta cagccgctct tcgtaatcta cggtcagcgg
     4861 gaaaaagtcg agcggccgcg gtcccgggct tgccgtagcc gtcacaagca cgaccgtgtc
     4921 gccgtagcgt acggtgacgg cgcctccggc ctgcgcagcg agggtgcctg tctctatcca
     4981 gaggggccgt ccgttgattt cagtttctac gtattctggc attggttgct tgttctccat
     5041 tgctcgactg cgtcatgcag gagcggggcc tttcggccgc taagggctgg tacgagggat
     5101 cattcgacct gcggcaggcg cgaagccgca cgccgatgcc tggggcttgt cgggcggtct
     5161 gggattgtgc gatcggtatg aacattgtga cgtgtggaac gaaaatggcc cccgtgatta
     5221 ctttcggagg cccaggcgag cgatgacgcc gcggtagcgc tcgacgtcgg tctggttgag
     5281 gtacgcgagc tgccgtcgcc gctggcccac gagcttgagc aggccccgcc gcgagtggaa
     5341 atcttgtttg tgggtgcgca tatgatcggt gagctggagg atgcgctcag tgagcaatgc
     5401 gatttgtact tccgacgagc cggtgtcctt ctcgtgggtc ctgtaggact ccacgaccga
     5461 gtctcttttc tccttggtga gcatgcgcct ttctttcggt gcccgccccc gcagtcgggc
     5521 cgtttact
//
LOCUS       DATCPE010000037         6273 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U7219
            k141_168491, whole genome shotgun sequence.
ACCESSION   DATCPE010000037 DATCPE010000000
VERSION     DATCPE010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955033
            Sequence Read Archive: SRR1586279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 6273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 16:49:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 640
            CDSs (total)                      :: 631
            Genes (coding)                    :: 627
            CDSs (with protein)               :: 627
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6273
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168491"
                     /isolate="SMAG_U7219"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            374..1984
                     /locus_tag="VIM45_01155"
     CDS             374..1984
                     /locus_tag="VIM45_01155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8819926.1"
                     /translation="MFEVSSEPNGTGSNGADPTPMALRDDIMPAMELPRVPRPLPRTP
                     PRESMPVEMPDWVDSRAHKRDWRQLFRRSRSGSKTNYEGDGVENVPGTTLAAYTPNLQ
                     PIEQTPPPKTRKSMKPLPWGRDFAAATIHRDDTLTAIFGKLDAADSPCVALVLPRGNQ
                     ELSKPLGMRRLRRHVDVSGKDVILVTHAGALRARAREVGLAVVGSTKAVDFERYGRNG
                     VKVGGVVVPLPGLGMFIRFCGLAAGTVALAAVVLLYLPTATVQVYPQLTLLSSTSDIL
                     LSTDATVVNAAGGEVPAHRRNTTITRTVPFPVHGQATVKGPDGSDQNVPAATDDDLKQ
                     GAQFAQQVLMDQGRTALAARYTGETLFPQTASVSALDIKPNVKANEPADLLEITATGQ
                     VVMLSADNESLQAILKRALQGKVDGSQMFVPETFTATPLAAGNFDKANNRLQTRVKLS
                     EGVTRAFSLKRLQQAVSGKGIRDAQQAVVERVDEVQPAKIKLNPGWAPWLPRFTSRIT
                     VKVSQVTVNASGDSAGASAGATPTPAAR"
     gene            2021..2461
                     /gene="ruvX"
                     /locus_tag="VIM45_01160"
     CDS             2021..2461
                     /gene="ruvX"
                     /locus_tag="VIM45_01160"
                     /inference="COORDINATES: protein motif:HMM:NF015607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEY8819927.1"
                     /translation="MTRLLGVDAGDERIGLAVGDDEGRMAVPFAIITRKGRGADWAAR
                     AVAVRARECGAEAVIVGLPLNMDGTFGPQAVKARSLGRKIALAAGLRLEWWDERMSSF
                     ISEQKQRELTKRGGRRRPVDDLAAAVILQSYIDSQSHVDSEKPN"
     gene            2458..3534
                     /gene="mltG"
                     /locus_tag="VIM45_01165"
     CDS             2458..3534
                     /gene="mltG"
                     /locus_tag="VIM45_01165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEY8819928.1"
                     /translation="MSRRVLPYIGLAIALAFVFIGYQASRNVAGWALPSAQKLPIDPT
                     SDSSRTTSLAINKGENARSIGAKLQTAGVIRSASWFRMLAEIEGVENDLAAGNYTFQL
                     DSDTQAVLDRVKVGILEPQILVTIPEGLRIEQVADLLEKKGVFQGQALLDQMKGGQFN
                     GDLLTDRPPGSSLEGYVFPDTYYFPKKSTADGVVNEMLHALDDRFDPELRGAIQAQGL
                     TDFQTLTLASIVEREAQVASERPMIASVFLNRMKQGIPLQADPTIQYAVAQDPASIAA
                     NGWWKRDLTVDDLKINSPYNTYANKGLPPGPICNPGRDAIAAVAHPAQTNFLYFVAKG
                     DGSHAFAATLEEHNANIQKYQKGR"
     gene            3531..4388
                     /gene="aroE"
                     /locus_tag="VIM45_01170"
     CDS             3531..4388
                     /gene="aroE"
                     /locus_tag="VIM45_01170"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013703921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEY8819929.1"
                     /translation="MTAGPRLAGLIGHPVAHSISPAFQQAAFDALGIHATYERWDTPP
                     DRLATRVRALRGARYFGANVTVPHKEAVLPLLDDLDPMARRAGAVNTIVNANGRILGS
                     NTDIVGFRRALREAGGFDAAGKRALVLGAGGAARAVILALEQEGAVSVAVANRHLARA
                     ERLVSDLRSNAGPGLIAIPWEQATSALTVASVDLLINCTTLGLAGSALARESPIPCEA
                     LHSDLYVCDIVANPLVTPLLHDASVAGAGCVGGLPMLVYGGAASFEQWTGETAPVDTM
                     LHAASMAMR"
     gene            4419..5150
                     /locus_tag="VIM45_01175"
     CDS             4419..5150
                     /locus_tag="VIM45_01175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8819930.1"
                     /translation="MGWGILGVSLLGLFLGFVIVQEMFVQRHWRGLVNRGDRWAIREL
                     VEQEIERWREMRTPKGTSASIWHGIQTTEVANVGRDFINLISSAEGEYRVVEGRRTEV
                     SSPLDEGMKLAAALLERLFYDVPNVSLSVVRVDVYTTFRGEDGAPDQQCILSTVGERS
                     VADRLPWDELRPNEIISRFESRFRLSENGALTPIDPGPVLREEDDEDGAGNEIPQRLS
                     TPTESAVTSKDSTPAAYPQDRRRQD"
     gene            5155..>6273
                     /locus_tag="VIM45_01180"
     CDS             5155..>6273
                     /locus_tag="VIM45_01180"
                     /inference="COORDINATES: protein motif:HMM:NF013375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional shikimate kinase/3-dehydroquinate
                     synthase"
                     /protein_id="HEY8819931.1"
                     /translation="MNNRHAPRRIALVGLPGSGKTSVARVLARRLGRPWRYVDTDELV
                     VALDGRSVPQIFRDSGEEAFRNVERTAIAQQERSEYAVIATGGGAVLDPINRRRLWQS
                     ALVVHLKARPETLASRVLGSGSNTGRPLLAGSDQSARLLELDEQRGPLYNLADWTIQT
                     DALYPDQVAEEILRVHQRLGEQLAARQGRERAWIETDTGGGGSAAAQVSTPSGSYSIY
                     TGPGELSRLGERMHAVGLKGRATVVADASVAPLYGEQVLSALRTEGFDAELVTIAGGE
                     QNKHLGEVARVYDRLIDRRSERGDTVVALGGGVVTDLAGFVAATYLRGVPLVHVPTSL
                     LGMVDASIGGKVGVDHALGKNLIGAFYQPRLVVADVDVL"
BASE COUNT         1235 a         1891 c         2019 g         1128 t
ORIGIN      
        1 ttggtagcgg ccgaggccag gggcggcggg cgacctgaac ttgcccaagg cggaatcaac
       61 aacccggccg cgctggaccg ggcattcgag gatgccaggc ggttagtcaa ggagttgctc
      121 tctgactgat gctacgggcg ataaagaaca aattgggcct tcaccactag gtgcgcgccc
      181 gaaaccgcca cctgcgagta aaacgccgat agcggatagt gcaggcaatg gcgttcggtc
      241 gcccgaaaat cgcacgcctc cgaagcagga gatgactcac cccgcgccag cgcagcgggc
      301 ggggcggcaa tccgccgcac ctccgccgcc tcccgcaacc gagggcgaga ccccggcgac
      361 agtgaaccaa cccatgttcg aggtgtcatc ggagccaaat ggcaccggtt ccaacggcgc
      421 ggatccaact ccgatggctc tccgcgacga catcatgccg gcgatggaat taccgcgcgt
      481 accgcggccg ctgccccgca caccgcctcg tgaatcgatg ccggttgaaa tgcccgactg
      541 ggttgattcg cgggcgcaca aacgagactg gcgacagctt ttccgacggt cgcgaagcgg
      601 ttcgaagaca aactacgaag gtgacggcgt agagaacgtt cctggaacca cgctcgcggc
      661 gtatacacca aatttacaac cgatcgagca gacgccgccg ccgaagaccc gcaaatcgat
      721 gaaaccgctg ccatggggcc gtgactttgc cgccgcaacc attcatcgag acgataccct
      781 gaccgccatc ttcggcaagc ttgacgcggc tgatagcccg tgcgttgcac tggtcctgcc
      841 gcgcggcaat caggaactgt ccaaaccgct tggcatgagg cggctgcgac gccatgtcga
      901 cgtttctggc aaggacgtga tactggtcac ccatgccgga gcactgcgtg ctcgcgcccg
      961 agaagtcggt ctcgccgtgg tgggcagcac caaggctgta gacttcgaac gctatggccg
     1021 caacggcgta aaagtcggcg gcgtggtggt accgctcccc ggcctcggca tgtttattcg
     1081 gttttgcggt ctggctgcgg gcaccgtcgc gttggcggcc gtcgtactac tgtacttgcc
     1141 taccgcgacc gtgcaggtct acccgcaact gacattgctc tcatccacat ccgacatttt
     1201 gctctcaacc gacgcgaccg tggtcaacgc cgcgggcggt gaagtgcccg ctcaccggcg
     1261 caacacgacg atcacgcgaa ccgtaccatt tccggtgcac ggccaggcca cggttaaagg
     1321 cccggacggc agcgatcaga atgttcccgc tgcgaccgat gatgacctta aacaaggggc
     1381 tcaattcgcc cagcaggtat tgatggatca gggtcgaact gcgctcgccg cgcgctatac
     1441 cggagagact ctattcccac agaccgcgtc cgttagcgcc ctggacatca agccaaacgt
     1501 caaggcaaac gaaccagccg acctgctgga gatcacggcg accgggcagg tcgtgatgct
     1561 cagcgcggac aacgagtcgc tgcaggcgat cctgaaacga gcgctccagg gcaaagtaga
     1621 tggcagccag atgttcgtgc cggagacatt taccgcgact ccgctggctg cgggcaactt
     1681 cgataaggcc aataatcgat tgcagacacg tgtcaagctc tcagaaggtg taacgcgggc
     1741 atttagcctc aagcgcttac agcaggccgt atcgggcaaa ggcattcgcg acgcccaaca
     1801 ggcggtggtg gagcgcgtgg acgaggtcca gccggcaaag atcaagctca atccgggctg
     1861 ggcgccatgg ctgcctcggt ttaccagccg catcacggtc aaggttagcc aggtgaccgt
     1921 gaacgcgtct ggtgattcgg ccggagcgtc cgccggcgcg acgccgacac cggctgcacg
     1981 gtaaagccac gtatattcga gttgtccggg agtcaaagcg atgacccgcc tcctcggtgt
     2041 cgatgccggt gacgagcgga tcggactcgc cgtcggcgac gacgaaggac gcatggcagt
     2101 gcccttcgct atcattactc ggaaggggcg cggagcagat tgggctgcgc gcgccgtcgc
     2161 cgttcgggcc cgcgagtgcg gcgcggaggc cgttatcgtc ggcctgccgt tgaacatgga
     2221 tggtaccttc ggcccccagg cagtaaaggc aaggtcactc ggtcgcaaga tcgccctggc
     2281 ggcggggctg cggctcgaat ggtgggacga acgcatgagc agtttcattt cggagcagaa
     2341 acagcgtgaa ctgaccaaac gtggcggacg gcgccgtcca gtggacgatc tggccgcggc
     2401 agtgatcttg cagtcctaca tcgattcgca atctcacgta gattcggaaa agcccaattg
     2461 agccgtcgcg tcctgccata catcgggttg gctatcgcgc tcgcctttgt gttcatcggc
     2521 taccaggcaa gccgtaacgt cgccgggtgg gcgttgccgt cagcgcagaa gcttccgatc
     2581 gatccaacgt cggacagttc ccgcaccacg agcctggcga taaacaaggg agagaatgcc
     2641 cgttcaatcg gcgcaaagct gcaaactgcc ggcgtgattc gaagcgccag ttggtttcgc
     2701 atgttggccg agatcgaagg ggtcgaaaat gacctggcgg cgggcaacta taccttccaa
     2761 ctggactctg acacgcaggc tgttttggac cgcgttaagg tcggtatcct cgagccacag
     2821 attctggtga ccattccgga aggtctacgc atcgaacagg tcgccgacct tctggagaag
     2881 aagggtgtgt tccagggaca ggcgctttta gaccagatga aggggggtca gttcaatggc
     2941 gacctgctca ccgaccgacc tccgggctcg agcctcgaag ggtacgtttt tcccgacacg
     3001 tactattttc cgaagaagtc gaccgccgac ggcgttgtca acgagatgtt gcacgcgctc
     3061 gatgaccgct tcgacccgga gttgcgcggt gcgatacagg cgcagggact aaccgatttc
     3121 cagacattga cgctggcgtc gattgtagag cgcgaagccc aggttgcctc ggagcggcct
     3181 atgatcgcct cggtgtttct caaccgcatg aagcagggca tccccttgca agccgatccc
     3241 acaattcaat atgcagtggc gcaagacccg gcgagcatcg cggcgaatgg atggtggaag
     3301 cgcgacctga ccgtggacga cctcaagatc aactctccgt acaacaccta cgcgaacaag
     3361 ggtctgccgc cgggcccaat ctgtaacccc gggcgcgacg cgatcgccgc agtcgctcat
     3421 ccggctcaga cgaacttctt gtacttcgtg gccaagggtg acggcagtca cgcgtttgcc
     3481 gcgaccttgg aggaacacaa cgccaatatc cagaagtatc aaaagggacg atgacagcag
     3541 ggccgcgact cgcggggctg atcggccatc cggttgccca ctcgatctca cccgcattcc
     3601 agcaagctgc ttttgatgcg ctgggcatac acgcgaccta tgaacgctgg gatacgcctc
     3661 cagaccggtt ggccacgcgc gttcgagcct tgcggggcgc tcgctacttc ggtgcgaacg
     3721 taacggttcc tcataaggaa gccgtgctcc cgttgctgga cgatctggat ccgatggcgc
     3781 ggcgcgcggg cgcggtcaac acgattgtca acgcgaacgg ccgcatcttg ggttcgaaca
     3841 cggacatcgt ggggtttcgg agggcgttgc gcgaagccgg cggttttgac gccgctggca
     3901 aacgcgccct ggtgctcggc gcgggtgggg cggcgcgggc ggtgattctg gcgttagaac
     3961 aggaaggcgc agtttccgtg gccgttgcga atcgccacct agcgcgggcg gaacggttgg
     4021 taagcgacct tcggtcgaat gcaggtccag ggctaattgc tattccctgg gagcaggcga
     4081 cgtcggcgct gacggtggcg agcgtggacc ttctgataaa ctgtacgacg ctcgggttgg
     4141 cgggttcggc gttggctcgc gaatcgccga tcccctgtga ggcgctgcat tcggacctgt
     4201 acgtgtgcga catcgtcgca aatccactgg tgacgccact gcttcacgac gccagcgtgg
     4261 caggcgctgg ttgcgtcggc ggtttgccga tgctggtgta cggtggagcg gcctcgttcg
     4321 aacagtggac cggagagacg gcgccggtgg atacgatgct acacgctgcg agcatggcga
     4381 tgcgctaacg ttcgggtgca ggaaagtagg acacggattt gggctggggt atccttggcg
     4441 tctcgttatt ggggctcttc cttgggtttg tgattgtcca ggagatgttc gtgcagcgac
     4501 actggcgcgg cctggttaat cgtggagacc gctgggcgat tcgcgaactg gtcgaacagg
     4561 agatcgagcg ctggcgcgag atgcgcacgc cgaagggcac gagcgcgtcg atctggcacg
     4621 gcatccaaac gaccgaagtt gcaaacgttg gacgcgactt catcaacctg atttcgagtg
     4681 cggagggcga ataccgggtc gtcgagggac ggcggaccga ggtaagctct ccgctggacg
     4741 aaggaatgaa actggccgcg gcattgctgg aacgcctatt ctacgacgtg cccaatgtga
     4801 gcctatctgt ggttcgcgtc gacgtctata cgacctttcg cggggaggat ggggcgcccg
     4861 atcagcagtg catccttagc accgttggcg agcgctccgt tgcagaccga cttccctggg
     4921 atgagctacg gcctaatgag attatcagta gattcgaaag ccgctttcgt ctcagcgaga
     4981 acggcgctct tacccccata gacccggggc cggtcctccg cgaagaagat gacgaggacg
     5041 gcgcggggaa cgagatcccc caacgcttat cgacaccgac cgaatccgca gtaaccagca
     5101 aagactcgac gccggccgcg tacccgcagg atcgacggcg ccaggactga aaaggtgaac
     5161 aatcgccacg cgccgcggcg gatcgccctc gtcggcctgc ccggttcggg caagacctcg
     5221 gtggcacgcg tcctcgcccg ccggctcggc cgtccctggc ggtacgtgga caccgacgaa
     5281 ttggtcgtcg cgctcgacgg acgttccgtt ccgcagatat tccgcgatag tggcgaggag
     5341 gcgtttcgaa acgtcgaacg cacggcgatc gcgcagcaag agcgcagtga gtacgcagtg
     5401 atcgccactg gcggcggcgc agtattggat ccgatcaacc ggaggcgtct ctggcaaagt
     5461 gcactcgtgg tgcatctaaa ggctcgtcct gaaacgctgg cgagccgcgt cctgggctct
     5521 gggtccaata ccggcagacc gctgctcgcc ggaagcgacc agtccgcccg attgctggag
     5581 ctagatgaac agcgtggtcc gctgtataac ctcgccgact ggacgatcca gaccgacgcc
     5641 ctttaccccg accaggtagc tgaagagatc cttcgcgttc atcagcgatt gggtgaacaa
     5701 ttggcggcac ggcagggacg cgagcgtgcg tggatcgaga ctgacaccgg cggtggtggt
     5761 tccgcggcag cccaggtttc cactccttcg ggctcatact ccatttacac cggaccgggc
     5821 gagctttcac ggttgggcga acgcatgcac gctgtcggac tcaaaggcag ggccacggtc
     5881 gtcgccgacg cgtccgtcgc gccactttac ggcgaacagg tactttcggc gctgcgaact
     5941 gaagggttcg atgccgaatt ggtgaccatt gccggtggcg agcaaaacaa gcacctaggc
     6001 gaggtcgcgc gcgtttacga tcgcctgatc gaccgccgta gcgagcgcgg cgacaccgtc
     6061 gtcgcgttag gcggcggcgt ggtcaccgat ctggccggct tcgtcgctgc gacgtatctg
     6121 cgcggtgtgc cgcttgtcca cgttcccacc agcctgctcg gcatggtgga cgcctcgatc
     6181 ggcggcaaag tgggagtcga tcatgctctc ggcaagaacc tgatcggcgc gttttaccag
     6241 ccacgcctgg tggtggccga tgtcgatgtc cta
//
LOCUS       DATCPE010000038         4570 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U7219
            k141_297744, whole genome shotgun sequence.
ACCESSION   DATCPE010000038 DATCPE010000000
VERSION     DATCPE010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955033
            Sequence Read Archive: SRR1586279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 4570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 16:49:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 640
            CDSs (total)                      :: 631
            Genes (coding)                    :: 627
            CDSs (with protein)               :: 627
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4570
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297744"
                     /isolate="SMAG_U7219"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1250)
                     /locus_tag="VIM45_01185"
     CDS             complement(<1..1250)
                     /locus_tag="VIM45_01185"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY8819932.1"
                     /translation="MPDDQNHVGLSPGAVPKSGWLPALHELAIAVAAGGSVPDLLQKV
                     AESSTVLSGAESGSLFLWDMWNAELQLAAFHKVALPDGRFQHDPGEDFARIIWERGEP
                     LIVNGFKHRPAAGDEALNSEVRSVIGVPLRVGGYRAGVLLALRHSEGRPFGVEDELAF
                     SLLALQAAHIVERSRANEALQASESHLRSLMQYTSDVISVLDVEGTVRYVSPSILRVM
                     GYPPQAITGATAFGAIHPDYVESVRGLFAEVVAASGAVRAIEFQRQRRDGSWMHVEGT
                     FHNLLDDPDVRGIVMNTRDITERKQVAEALRHQALHDALTGLPNRSLLVERLDQAIDA
                     AEREERPLAFLKLDLDRFKQVNDSFGHQTGDQLLQQAIPRLRANLRGADTVARLGGVE
                     FAVLLPDSGPEGAAVTARELLLAL"
     gene            1500..1688
                     /locus_tag="VIM45_01190"
     CDS             1500..1688
                     /locus_tag="VIM45_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003084246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YezD family protein"
                     /protein_id="HEY8819933.1"
                     /translation="MTAERIDLSTATPGVRPGPEELLVLNEVLSAIRGVRHGTVTLIV
                     QDGRVVQIDRTEKRRLVR"
     gene            1766..2455
                     /locus_tag="VIM45_01195"
     CDS             1766..2455
                     /locus_tag="VIM45_01195"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005544498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HEY8819934.1"
                     /translation="MDTELLQSLNERFAGGSPQQLIAWAAETYGGRLALSCSFGGASG
                     MVLLDLALQIDRSVAVVYVDTDYLFPETYETVRAVEGRYGINVLGFRSSLSPKTQELL
                     CGPALWLRDPDRCCELRKVEPMRAALANYDAYLTGLRRDQAGTRSETPLVQWDAKFGL
                     YKLNPLAEWSERDVWSYIVANKLPYNPLHDQGYPSIGCTNCTRPVTAGEDPRSGRWSG
                     TDKVECGLHTS"
     gene            2478..3425
                     /gene="cysD"
                     /locus_tag="VIM45_01200"
     CDS             2478..3425
                     /gene="cysD"
                     /locus_tag="VIM45_01200"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017592260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HEY8819935.1"
                     /translation="MTATYGIAAAAPAGKPVRPSHLAVLEAESIFVLREAVAEFQRPV
                     LLFSGGKDSAVLLRLAEKAFRPAKFPFPLMHIDTGFNFPEVIDFRDRRAAELGEDLVI
                     RYVQDSIDRGRVVEETGPTASRNRLQTTTLLDALAEFRFDVAFGGARRDEEKARAKER
                     MFSFRDEFGQWEPKAQRPELWTLYNGRIRRGEHVRTFPLSNWTELDIWQYIALERLEL
                     PSVYYAHRRLVVEREGMLLAAGEHLQLLPAELPFEAEVRYRTVGDMSCTGAVLSSACR
                     AEQIIGEVAASHLSERGATRADDRTSDAAMEDRKREGYF"
     gene            3426..>4570
                     /locus_tag="VIM45_01205"
     CDS             3426..>4570
                     /locus_tag="VIM45_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013839483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEY8819936.1"
                     /translation="MQVTSPPIYDGTDLLRFSTAGSVDDGKSTLIGRLLYESKAIFQD
                     QLAEVERASRAGAGEYVNLALLTDGLRAEREQGITIDVAYRYFATSRRKFIVADTPGH
                     TQYTRNMVTGTSTADVAIVLVDAARGITEQSRRHLYVAALVRVPHLIICINKMDLVGF
                     AEPVYEQLRREVVDYASRLDVGDLTFVPISALRGDNVVERSEHMPWYEGPALLPLLEE
                     IEVAESAAPVGARFPVQLVNRPAIGAFEHHRRYAGQIAAGVLRPGDPVVALPSGIETY
                     VRSIETFDGPLAEAGPPLSIALTLEDDIDISRGDMICSAADQPRAEKEVDAMVCWMSR
                     TPMQEGDRYAIKHTTRSARAIVQELRYRVDMNTLAEQDEVTSLGLND"
BASE COUNT          875 a         1510 c         1378 g          807 t
ORIGIN      
        1 tccagcgcca gcaacagttc acgcgccgta accgccgcgc cctcgggacc agaatcgggc
       61 aacaataccg cgaactccac accgccgagc cgggccaccg tatcggcgcc ccgcaggttc
      121 gcccgcagcc gggggatcgc ctgctgcagc agttgatcgc cggtttgatg gccgaaactg
      181 tcattgacct gtttgaagcg gtccaggtcg agcttcagaa atgccagcgg tcgctcttcg
      241 cgctcggccg catcgatcgc ctggtccagt cgttcgacca gcaacgaacg gtttggtagc
      301 cccgtcaggg catcatggag cgcctggtgc cggagcgcct ctgccacttg cttgcgctcc
      361 gtgatgtcac gcgtgttcat gacgatgccg cgcacgtccg ggtcgtcgag caagttatga
      421 aaggtcccct cgacgtgcat ccatgaccca tcgcgccgtt ggcgttggaa ttcaatcgcc
      481 cgcaccgccc ccgatgccgc gacgacctca gcgaacagcc cgcgcacgct ctctacgtaa
      541 tccggatgaa tggcgccaaa cgccgtcgca ccggtaatcg cctgcggcgg gtatcccatt
      601 acccggagga tcgaagggct gacgtaccgg acggttccct ccacgtccag caccgatatg
      661 acgtcagaag tgtactgcat caacgatcgc agatgcgatt cactcgcctg tagcgcctcg
      721 tttgcacggc tgcgttcgac gatgtgcgcc gcttgcaacg ctagcagcga aaacgctagc
      781 tcgtcctcaa ctccgaacgg ccggccctct gaatggcgca gcgccagtaa aacgccggcg
      841 cggtaaccac cgacacgcaa gggaacgcca atcaccgatc gcacctcgct attaagcgcc
      901 tcgtccccgg ccgccggccg gtgcttaaaa ccattgacga tcagcggttc gcctcgctcc
      961 cagataatcc gggcaaaatc ctcgccggga tcgtgctgaa accgtccgtc cggcagagca
     1021 accttgtgga aagctgccag ttgcagctct gcattccaca tatcccagag aaagagcgat
     1081 ccgctctcgg cgccgctgag cacggtcgag ctttcggcca ccttctggag caggtcgggg
     1141 acacttccac cggccgctac cgcgatcgcc agttcgtgca gcgccggcag ccagcccgat
     1201 ttcgggaccg cgccggggga tagtcccacg tggttctggt cgtcgggcac cggcttacct
     1261 cgcgttcgcc ataccggcag cccgcacggc tccaagcggg agaacgcacg ctacggatac
     1321 gtcatgctac cacccggtta ccgggatgtc ccgcctgcag taagtatcgg atgctatgac
     1381 cgaattatta ccgccgtccc tcatacgcgg cggcggttca aacccagcag gttgacaaca
     1441 ccgaagtgca cctgtaaact tccgagaaag tagacttagt gaatcaagaa actacactta
     1501 tgaccgcgga acgcatcgac ctgtccaccg ccacgcccgg tgtccgtccc ggccccgagg
     1561 agctactcgt cctgaacgag gtgctttctg ccattcgcgg cgtgcggcat ggcaccgtaa
     1621 cgctgatagt ccaggatgga cgcgtcgtgc agatagaccg tacggagaaa cggcggctgg
     1681 tccgctagct cgaatatcgc ggctgaccgg taaaccggag gcccatcccc gtagtgggga
     1741 agggcctcta tctgtttgga gaacaatgga caccgagctt cttcagtccc tcaacgagcg
     1801 ctttgccggc gggtcgcccc aacagctcat cgcctgggcg gccgaaacct atggcggacg
     1861 cctggcgctc tcgtgttcgt tcggcggcgc aagcggcatg gtgttgctcg acctcgcgct
     1921 gcagatagac cggtcagtgg ccgtcgtcta cgtggacacc gactatctct tccccgagac
     1981 gtatgaaacc gtgcgcgctg tcgaggggcg ctacggcatc aacgtgctcg gctttcgctc
     2041 ctccctctcg ccaaagacgc aggagctgct ctgcggacct gctctctggt tgcgtgaccc
     2101 ggaccgctgc tgcgagctgc gcaaagtcga gccgatgcgc gcggcactgg ccaactacga
     2161 cgcgtacctt accggcctgc gccgggacca ggcgggaact cggtcagaaa ctccgctcgt
     2221 gcagtgggac gcaaagttcg gtctatacaa gctcaacccg ctggccgagt ggagcgagcg
     2281 tgacgtttgg tcctacatcg tcgccaacaa gctcccttac aacccgctgc acgaccaagg
     2341 atacccgagc atcggctgca ccaactgtac gcgaccggtg accgcgggcg aagacccgcg
     2401 ctcgggtcgc tggagcggaa ccgacaaagt tgagtgcggg ctgcatacgt cctgaaactg
     2461 aaacacgagg acctcgaatg accgcgacct atggcattgc cgccgctgcc ccagccggca
     2521 agccggttcg tcctagccac cttgccgtgc tggaagcgga atccatcttt gtcctgcgtg
     2581 aggcggttgc cgagtttcaa cggccggtgc tgctcttctc cggcggtaag gactccgcgg
     2641 tgctcttgcg cctcgcggag aaggccttcc gtcccgcgaa gttcccgttc ccgcttatgc
     2701 acatcgacac cggctttaac tttccggagg tcatcgactt tcgggaccga cgggcggccg
     2761 agctgggcga ggacctcgtc attcgttacg tgcaggacag catcgatcga gggcgggtcg
     2821 tcgaagagac ggggccgacg gcatcgcgga accgcctcca gacgaccacg ctgctcgacg
     2881 ccctcgccga gttccgcttt gacgtcgcat tcggcggcgc ccgtcgcgat gaggagaagg
     2941 cccgcgccaa agagcgcatg ttctcgttcc gagacgagtt cggacaatgg gagccgaagg
     3001 cgcagcgacc ggaattatgg acgctgtaca acggccgcat ccggcgtggc gagcatgtcc
     3061 gcacgtttcc gctttccaac tggaccgaat tggacatctg gcagtatatc gcgctggaac
     3121 ggctcgaact gccgagcgtc tactacgccc atcggcggct cgtcgtggag cgcgaaggca
     3181 tgctgctcgc tgcgggcgag catctccagc tcttgcctgc cgaattgcca tttgaggccg
     3241 aggtgcgtta ccggaccgtc ggcgacatgt cctgcaccgg tgcggtcctc tcttcggcgt
     3301 gccgcgcgga gcaaatcatc ggcgaggtcg ccgcgtccca tctctcggaa cggggggcaa
     3361 ccagggccga cgaccggaca tcagacgccg caatggaaga ccgtaagcgc gaagggtatt
     3421 tctaggtgca agtcacgagc cccccaatat acgacggcac ggatctcctg cggttctcca
     3481 ccgccggctc ggtcgacgac ggaaaaagca cgctgatcgg ccggctgttg tacgaaagca
     3541 aagcgatctt tcaggaccag ctcgcggaag tcgagcgagc cagccgcgcg ggcgccgggg
     3601 aatatgtcaa ccttgcgttg ctcaccgatg gccttcgcgc cgagcgcgaa cagggaatca
     3661 ccatcgatgt cgcctaccgt tacttcgcca cgtcgaggcg aaagttcatt gtggccgaca
     3721 cacccgggca cacgcagtac acgcgcaaca tggtgacggg tacatcgaca gccgacgtcg
     3781 ccatcgtctt ggtggacgcc gcccgcggca tcaccgagca atcgcgccgc cacctgtacg
     3841 tcgctgcgct ggtccgcgta ccacatctga ttatctgtat caacaaaatg gacctggtcg
     3901 gtttcgccga gccggtttac gagcagcttc gacgggaagt cgtggactat gcgtcccggc
     3961 tcgacgtagg ggatctcacc ttcgtcccga tttcagctct ccgcggcgac aatgtggtcg
     4021 aacgcagcga gcacatgccc tggtatgaag gaccggcgct cctgccgctc ctggaggaga
     4081 tcgaagtcgc cgagagcgcc gcacccgttg gcgctcgctt cccggtgcaa ctggtgaatc
     4141 ggccggcgat cggcgcgttc gaacaccacc gcaggtacgc cggccagatc gccgccggcg
     4201 tactgcgccc aggggaccct gtggtagcct tgccgtccgg catagagaca tacgtccgat
     4261 ctatcgagac gttcgacggt cccctcgcgg aagctgggcc accgctcagc atcgcattga
     4321 cgctcgagga cgacatcgat atctcacgtg gtgacatgat ctgctcggcc gccgaccaac
     4381 cacgggcgga aaaagaggtc gacgcgatgg tgtgttggat gtcccgcacg ccgatgcaag
     4441 agggcgatcg atatgcaatc aagcacacca cccgcagcgc ccgagcgatc gtgcaagagc
     4501 tgcgctaccg cgtggatatg aacacgctcg ccgagcagga cgaagttact tcgctcggcc
     4561 tgaacgacat
//
LOCUS       DATCPE010000039         3106 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U7219
            k141_557355, whole genome shotgun sequence.
ACCESSION   DATCPE010000039 DATCPE010000000
VERSION     DATCPE010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955033
            Sequence Read Archive: SRR1586279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 16:49:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 640
            CDSs (total)                      :: 631
            Genes (coding)                    :: 627
            CDSs (with protein)               :: 627
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3106
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_557355"
                     /isolate="SMAG_U7219"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..885
                     /locus_tag="VIM45_01210"
     CDS             <1..885
                     /locus_tag="VIM45_01210"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEY8819937.1"
                     /translation="YDPAQIDQSGDALLSDPGKPLLNRTTQGLYPPGSTYKTVTASAA
                     LDTGVVKPDDRFKCVNGVVIQGFVIQCTNAPQGQTEWDFLHAYAFSINATFAQVAAER
                     LGSDRFIAYSRKFGLDQPLSFDIDTAISRTSRSGGGLNQVLLASSGFGQGQLVVSPLQ
                     MALVTATVANHGIEPAPYLVQEIRDAAGNIVEEHSQTDRGRVMSEATAAEMVHFMETA
                     VNEGFGSETGLLGMGIAGKTGTAETGTDAPADAWFTGFAPSNAPSLVVVVEVDNGGPG
                     SRAAAPVAQQIFQAFLGQ"
     gene            979..1776
                     /locus_tag="VIM45_01215"
     CDS             979..1776
                     /locus_tag="VIM45_01215"
                     /inference="COORDINATES: protein motif:HMM:NF023818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FhaA domain-containing protein"
                     /protein_id="HEY8819938.1"
                     /translation="MKPLSRLESLIAEILERPAWTLSPHKLHPLELTAALTKALERRA
                     VRLVDRVLAPDRYEVSVHPDDLSAFGDAGRVLERELAEYLGRTITERDLTCNRPPSVR
                     LVADKSVRPGRVHVTAAFTARPDLRGDISGGFTRLSASAASRRSGTPVAMSGRDNPIT
                     GARPDAILEVLDGSGRTAQDFPVNGTPAIVGRRHDVTVPLLDSKVSREHARIDLTAAG
                     YTITDLQSLNGTLVNDREIAGTVALRDGDLIDIGHFRLRFRQRGSSQ"
     gene            1796..2233
                     /locus_tag="VIM45_01220"
     CDS             1796..2233
                     /locus_tag="VIM45_01220"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEY8819939.1"
                     /translation="MGALNDAVLVLVRLTVLALLYIFLTVVLRGVWKDFSTAAPARKG
                     NIGRAFLVVVEGPHEILKRGQRIPIEGAASIGRDSDNQVVIPDKTVSGRHAAIIYRDG
                     RWWAEDFDSTNGTWVNDRRVSSPLPVAEGDMIQVGRFSFRLAV"
     gene            complement(2241..>3106)
                     /locus_tag="VIM45_01225"
     CDS             complement(2241..>3106)
                     /locus_tag="VIM45_01225"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEY8819940.1"
                     /translation="TAVVGKEINGSRLWLGAGGFYFQVTEAMKLLLVLFLAGYLADRR
                     LMLAAVSRRWRSFRVPTLPYLVPLVIIWGFTLALMAWQHDLGAMLLLMGVTLLMLYVA
                     TGRWAFVGAGLAIVVLNLFVAYHAFGYVRVRIDLWLHPLTQIHGAGYQIAQSIFAFAG
                     GGILGTGLGRGYPGLIPVVQTDFIFAAIGEELGTAGAFAIVGVYVVMAFRGLRISVRQ
                     PADYGMLLAVGITAILAVQSLIIMAGNMALIPITGITLPFISYGGSSIVVNFILIGFL
                     LRLSTSRPPPG"
BASE COUNT          610 a          964 c          973 g          559 t
ORIGIN      
        1 tacgacccgg cgcagataga ccagagcggg gacgcgctcc tctccgatcc gggaaagccc
       61 ctgttgaacc ggactacgca gggcctttac cctccgggat cgacatacaa gacggtcacc
      121 gcttcagccg cgcttgatac cggtgtcgtg aagcccgatg atcggttcaa gtgtgtcaac
      181 ggcgtcgtca tccaggggtt cgtaattcag tgcaccaacg cgccgcaggg gcagaccgag
      241 tgggattttc tgcatgcgta cgctttctcg atcaacgcga cgtttgccca ggttgcggct
      301 gagaggctgg gctccgaccg cttcatcgcc tacagccgca agttcggtct ggaccagccg
      361 ctgtcgttcg atatcgacac cgcgatcagc cgcacgtcgc gttcgggcgg cggcttgaat
      421 caggttttgc tcgccagcag cggcttcggt cagggacagc tagtggtatc gccgctgcag
      481 atggcgcttg taacggcaac cgtagccaac catgggatcg agccagctcc ctacctggtg
      541 caggagatcc gtgatgccgc tggcaacatt gtcgaggagc actcgcagac cgaccgcggc
      601 cgcgtcatgt cggaagcgac cgccgcggag atggtgcatt tcatggagac ggcggtgaat
      661 gagggtttcg gttccgaaac aggactgctc ggaatgggca tagccggcaa gacgggcacc
      721 gctgagacgg gcaccgacgc gccggccgac gcctggttca ccggctttgc gccgtccaac
      781 gcgccgtcgc tcgtcgtcgt cgtcgaggtc gataatggag ggccaggcag ccgggccgct
      841 gcgccagtgg cccagcagat attccaggca ttcctcggtc agtgaacgcc ggaacagaag
      901 cagccggtcc ggatggtata atcggaccgg aacggcagag catacttcgg ccgatcactc
      961 tgtgttcacg gcgggcacat gaagcccctt tcgcgtctcg agtcgctcat cgccgaaatc
     1021 ctggagcgac ccgcctggac gctttcgcct cacaagctcc accccctcga acttaccgcg
     1081 gcgttgacca aggcgttgga gcggcgcgcc gttcgtctgg ttgaccgcgt gcttgcgcct
     1141 gatcgctacg aggtctccgt acatccggat gatctctctg cgttcggcga cgccggacgc
     1201 gtgctcgaac gcgaattggc cgaatatctc ggtcgcacga ttaccgaacg cgatctgacc
     1261 tgtaaccgtc ccccgtccgt gcgactggtc gccgacaagt ccgttcgacc cgggcgcgtg
     1321 cacgtgacgg ccgcgttcac ggcgcgtcct gatctccgcg gagacatttc cggtggtttc
     1381 acaaggctat ccgcctccgc tgcttcgcgg cggagtggta cgcctgtcgc gatgtccggt
     1441 cgcgataacc cgattacggg agcccggccc gatgcaattc tcgaagtgtt ggatggcagc
     1501 ggccgcactg cgcaggattt tccggtgaac ggtaccccgg cgatcgtcgg gcgacgccat
     1561 gacgtgacgg tgccgttgct tgacagcaaa gtctcgcgcg agcacgcgcg catcgatctt
     1621 accgccgcgg gttatacgat tacggacctg cagagcctga acggcacctt ggtcaacgac
     1681 cgagagatcg caggaaccgt cgcactgcgc gacggggacc tcatcgacat cggccatttc
     1741 cgcctccgtt ttaggcaacg cggatcatca caatgatcac gttcattccg gacgggtggg
     1801 tgctctgaac gacgccgttc tcgtgctcgt gcgcctgacc gtgcttgcgc tgctttacat
     1861 ttttctaacg gtggtccttc gcggggtgtg gaaggacttc tcaaccgcgg cgccggcgag
     1921 aaagggcaat atcggacggg cctttttggt cgtcgtcgaa ggtccccacg agatattaaa
     1981 gcggggtcag cggataccga ttgagggcgc cgccagcatt ggccgcgata gcgacaacca
     2041 ggtcgtcatt ccagacaaga cggtttcggg ccgtcacgcg gcgatcatct accgcgatgg
     2101 ccgttggtgg gcggaagact tcgatagcac gaatggcacc tgggtaaacg accggcgcgt
     2161 ttcttcgccg ctgccggtcg cggaaggcga catgatccag gtgggacgat tctcttttcg
     2221 tctggcggtt tagaaccgtc tcaccccgga ggcggccgcg acgtggacag ccgcagcagg
     2281 aagccgatca ggataaagtt gacgacgatc gacgatccgc cgtagctgat gaacggcaag
     2341 gtaatcccgg taatcgggat taaggccata ttcccagcca tgatgatcag cgattgaacc
     2401 gcgaggatgg cggtaattcc aaccgcaagc agcatgccgt aatccgcggg ctggcgcacg
     2461 gaaatccgca ggccgcgaaa ggccatgacc acgtagacgc ccacgatcgc gaaggcgccg
     2521 gcggtgccga gctcctcgcc tatggctgcg aagatgaagt cggtttgcac gaccgggatc
     2581 aagcccggat atcctcgacc aagcccggtg cccaagatgc cgccaccggc aaacgcgaag
     2641 atcgactgcg caatctggta gccggcgccg tgtatctgcg taagcgggtg cagccagagg
     2701 tctatacgga cccggacgta gccaaaggcg tggtacgcga caaagaggtt gagtacgacg
     2761 attgcaaggc cggcgccgac aaatgcccat cgcccggtgg cgacgtagag catcagcagc
     2821 gtaacaccca tgagcagcag catggccccg aggtcgtgtt gccaggccat cagtgcgagc
     2881 gtgaagcccc agattatgac cagcggcacc aggtagggca gcgtcggcac ccgaaagctg
     2941 cgccatcgcc tgctgaccgc tgccagcatc aagcgccggt cggcaaggta gccggctaaa
     3001 aatagcacca gcaaaagctt catcgcttcg gtgacctgaa agtagaagcc acccgcgccc
     3061 agccacagcc gcgatccgtt gatctcctta ccaactaccg ccgtaa
//
LOCUS       DATCPE010000040         5796 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U7219
            k141_200810, whole genome shotgun sequence.
ACCESSION   DATCPE010000040 DATCPE010000000
VERSION     DATCPE010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955033
            Sequence Read Archive: SRR1586279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 5796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 16:49:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 640
            CDSs (total)                      :: 631
            Genes (coding)                    :: 627
            CDSs (with protein)               :: 627
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5796
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200810"
                     /isolate="SMAG_U7219"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2246)
                     /gene="gyrA"
                     /locus_tag="VIM45_01230"
     CDS             complement(<1..2246)
                     /gene="gyrA"
                     /locus_tag="VIM45_01230"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEY8819941.1"
                     /translation="MVVEQTGDRITPVRIEDEMRQSYLDYAMSVIVQRALPDVRDGLK
                     PVQRRILYSMSEMGLRSSAAYKKSAAVVGEVMGKYHPHGDAPVYDAMVRMAQDFSLRY
                     PLIDGQGNFGSVDADPPAAMRYTEAKLAQVADEMLADIDKNTVDFVPNYDGSHQQPES
                     LPARLPNLLVNGSSGIAVGMATNIPPHNLTEICNAIMRLIDKPDTTTEELTEIVRGPD
                     FPTAGIIIGREGIRNAYATGQGRVIMRARAHVEEMARAGRFQIVVTELPYQTNKASLI
                     EKMADLVKDKKIDGISDLRDESDRHGMRIAIELKAGGQPKQILNALYKHTSMQSSFNV
                     HMLCLVNGQPKTVTLRTALHEYLNFRRSVIRRRSQFDLDKAREREHIVAGLLKALDKI
                     GAIIRLIRGSESAAAAKVKLQEKPFTFTERQATAILAMTLSRLAALERQQLLNEHAEL
                     LKTITFLEELLADAAKVDAVIKEETLELRKKHGDDRLTQIVDQELQNFSDEDLIPHEE
                     IVVTISRRGYIKRLALDTYRPQRRGGKGLTGMPTREEDAVHRLLVADTHDNLLFFTDR
                     GKVFQLKAHEIPDASRTAKGLPLINLIDILQGELVTAVIATATFDGDCLLLATKRGEV
                     KRTPLKDFESVRRNGLIAMNLEQGDALVSVKMANSADDLVLATADGQCICFPVGELRM
                     ASRQSGGVRGIRLAKGDFVVGMEIAARGQHLFTISERGFGKRTDFAQYPRHHRGGSGV
                     SNLKLTDKT"
     gene            complement(2372..3073)
                     /locus_tag="VIM45_01235"
     CDS             complement(2372..3073)
                     /locus_tag="VIM45_01235"
                     /inference="COORDINATES: protein motif:HMM:NF019342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEY8819942.1"
                     /translation="MHTPKIAISVGSQGAAKGYDNYILRVTEAGAEPMIAAPVCDVEH
                     VLNGVDALLVTGGTDIDPALYGEQPAAELLTPDLPRDESEIALVRAALRRDIPVLAIC
                     RGHQVLNVALGGRLQQHIPGDGHRAFDEPPHDSRYHTVQVEEGSKLADLLGAGPHRVN
                     SRHHQAIRPDLLGEGLLPSAMSPDGIVEGIESPEHRFVVGVQWHPERPEVIDDFRALF
                     TALVEAARVASVSAT"
     gene            complement(3109..3927)
                     /locus_tag="VIM45_01240"
     CDS             complement(3109..3927)
                     /locus_tag="VIM45_01240"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8819943.1"
                     /translation="MIATQRALRSHNFNLPDLSHHALNVWRRNRDVYLKLWKTELIMP
                     LVEPILVLTAMGIGLGHFISLGGSGTYVAFLAPGILAQFGMFQATFECGWGAYFRMEN
                     QGTYTAVAATPATLDDIVAGEVMWAATRSVINSFYILAVLLALTPTYGIVQSPMAVLV
                     LPVAYLLGLCFGALTLCFTAMAPAISFLGYYFNVVIIPLFWLSGTFFPIERLPGWLQV
                     VAWATPPAQIIATFRHFLNGVPQWADLFHITLLLIETVVFGYIAMLLVRRKLIT"
     gene            complement(3924..4871)
                     /locus_tag="VIM45_01245"
     CDS             complement(3924..4871)
                     /locus_tag="VIM45_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018815530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8819944.1"
                     /translation="MQKHRQDHSVQALITARSLYKCYGDFTAVDHIDFDVLPGECFGF
                     LGPNGAGKTSTMRMTTCMSPPSGGELLVDGLDVRSDHRRIKARLGVVSQDDNLDGDLT
                     VRENLLVYARYFDISRAVAAARADEVLDLFQLTDKRNEKINALSGGLKRRLTIARALI
                     SAPTLLVLDEPTTGLDPQARHLVWRQLRALKDQGMTMVLTSHYMDEAARLCDRLVIMH
                     QGRILVQGTPEALVREHAGNEVIELRPAPGRTNEILAAAAMVEGLQIEDTGDTIYLFC
                     DVPVGSLAMRFEDAADDIFVRRANLEDVFLRLTGRGLLD"
     gene            complement(4890..5744)
                     /locus_tag="VIM45_01250"
     CDS             complement(4890..5744)
                     /locus_tag="VIM45_01250"
                     /inference="COORDINATES: protein motif:HMM:NF024820.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY8819945.1"
                     /translation="MVYRTDELSRLRKQRTEQAIRLAMQSRWEEAVTVNLSVLEMTGQ
                     RDGDAFNRLGRALMALGKYKEAREAYGRALKLDPTNQIAKKNIAALEKKAEIESDAGA
                     APRDKVDPKLFVEETGKTGLTVLQRPRKDALSHMTPGERVHLRPDGNLLLATNAAGDA
                     LGQIEPRIALRLINLMKGGNEYAAAISQLGSDTARIIIKETLQSAANSGRLSFPPAGV
                     DGTPRPYTREGLIRYDDEDEEILEEVETGEGWETDGEAHGQGDVTLLEYQKAHEGVVR
                     DDGTFEDE"
BASE COUNT         1057 a         1753 c         1782 g         1204 t
ORIGIN      
        1 gtcttgtccg ttagtttcaa gttggagacg cccgatccac cgcggtggtg cctcgggtac
       61 tgcgcgaaat cggtacgctt gccaaaacca cgctcgctga tggtgaacag gtgctgcccc
      121 ctggccgcga tctccatacc aacgacgaag tcgcccttgg ccagcctgat cccgcgcacg
      181 ccgccactct ggcgggaggc catgcgcagt tcaccgaccg ggaagcagat gcactggccg
      241 tcggccgtcg ccagcaccag gtcgtccgcc gagttcgcca ttttcacgga gacaagcgca
      301 tcgccttgct ccaggttcat cgcgatcagg ccgttacggc ggacactttc gaagtccttc
      361 agcggcgttc gcttgacttc gccccgcttg gtcgcgagca gcaggcagtc tccatcgaac
      421 gtcgccgtgg cgatgaccgc ggttacgagc tcgccctgca gaatgtcgat caggttgatc
      481 agcggcaggc ccttcgccgt gcgcgaagcg tccggaattt cgtgcgcctt gagctgaaag
      541 accttgcctc gatccgtgaa gaagagcaga ttatcgtgcg tgtcggctac aagcagacgg
      601 tgcactgcgt cctcttcacg cgtgggcatg ccggtcagcc ccttgccgcc acgccgctga
      661 ggccggtatg tatcgagcgc aagccgcttg atgtagcctc gcctgctgat ggtgacgacg
      721 atctcctcgt gaggaatgag gtcctcgtcc gagaagttct gaagctcctg gtccacgatc
      781 tgggtgaggc ggtcgtcgcc gtgcttctta cgcagctcga gcgtctcttc cttgatcacg
      841 gcgtcaactt tggccgcgtc ggcaagaagc tcctcgagaa acgtgatcgt ctttagaagc
      901 tcggcgtgct cgttcagaag ctgttgccgc tcgagcgcgg cgagccggct gagcgtcatg
      961 gccaggatgg cagtcgcctg ccgttcggtg aaggtgaacg gtttctcttg aagtttgacc
     1021 tttgcagcgg ccgcggactc cgacccgcga atcaacctga tgatcgcccc tatcttgtcg
     1081 agcgctttca gcaggccggc gacgatgtgt tcgcgttcgc gcgccttgtc gaggtcgaac
     1141 tgactgcgac ggcgaatcac gctgcggcgg aaattcaggt actcgtgcag cgccgtacgc
     1201 agggttactg ttttcggctg accgttgacg aggcagagca tatgcacgtt gaacgacgac
     1261 tgcatcgagg tgtgcttgta cagcgcgttc aggatctgtt tcggctggcc gccggccttg
     1321 agctcgatcg cgatgcgcat gccatgccgg tcggactcat cgcgcaggtc gcttataccg
     1381 tcgatctttt tatctttgac gaggtcggcc atcttctcga tcagcgacgc cttgttggtt
     1441 tgatacggca gctcggtaac gacgatctgg aagcgcccgg cgcgtgccat ctcttcgacg
     1501 tgtgcccggg cccgcatgat gacgcgacct tgaccggtcg cgtaggcgtt ccggatcccc
     1561 tcccgaccaa tgatgatgcc agcggtgggg aagtcgggcc ctcgtacgat ctcggtcaat
     1621 tcctcggtcg tcgtgtccgg tttatcaatc aagcgcatga tcgcgttaca gatctcggtg
     1681 aggttgtgcg gtgggatatt ggtcgccatg ccgaccgcaa tgccggacga gccgttcacc
     1741 agcaggtttg gcagtcgcgc cggcaacgat tccggttgtt ggtggctgcc gtcgtagttc
     1801 ggcacgaaat cgacggtgtt tttgtcgatg tcggcgagca tctcgtcggc gacctgcgcc
     1861 agcttcgctt cggtgtagcg catcgcagca ggtgggtcgg cgtcgacact gccgaagttg
     1921 ccctggccgt cgatgagcgg gtagcgcagc gagaagtcct gtgccatacg taccattgcg
     1981 tcgtataccg gcgcgtcgcc gtgcggatga tacttgccca ttacttcgcc gactacagcg
     2041 gcgctcttct tgtatgcggc gctggaccgc agccccatct cgctcatcga gtagaggatg
     2101 cgccgttgaa ccggtttgag gccgtcgcgc acgtcgggga gtgcccgctg cacgatgacg
     2161 ctcatcgcat aatcgagata cgactggcgc atctcgtcct cgatacgtac cggggtaatg
     2221 cgatcgccag tctgttctac aaccattccg tactcaaccc tcgctacaag ctatccaaaa
     2281 tgacagagag ccagcacagg ctcgaagcgg cagatatcac gccattcacg gcgcatcaat
     2341 ctcagagctg cttattggtt agggagcgca actacgtcgc ggacacgctc gctacccggg
     2401 ctgcttcgac aagcgccgtg aaaagcgcgc ggaagtcgtc gatgacctcg ggacgctcgg
     2461 ggtgccactg cactcccacc acaaaacgat gctccgggct ctcgataccc tcgacgattc
     2521 cgtccgggct catcgcggat ggcagcaacc cttcgccgag caggtccggg cgaatcgcct
     2581 ggtggtggcg ggagttcacg cgatgcggtc ccgcgcccag caggtccgcc aatttgctgc
     2641 cttcttcgac ttgaaccgtg tgatagcgcg aatcgtgcgg tggttcgtcg aaggcgcggt
     2701 gaccatcgcc cgggatgtgc tgctgcagcc ggccgccgag agcaacgttg agcacctggt
     2761 ggccgcggca aatcgccaga acgggaatat cgcgccgtag agccgcgcgc actaacgcaa
     2821 tctctgactc gtctcggggt agatccggtg tcaatagctc agctgccggt tgctcaccgt
     2881 acagcgcggg gtcgatgtca gtgccaccgg tcacgagcag cgcgtcaacg ccgttcagca
     2941 cgtgttctac atcacacacc ggcgccgcaa tcatcggctc ggcgcccgct tcggtgaccc
     3001 gcagaatgta attgtcgtag cctttagcag caccctggct gcctaccgag atagcaattt
     3061 tcggggtgtg catggcgcct ccacacacgt aattgtaaca gatctcttct atgtaatcag
     3121 cttgcgacga acgaggagca ttgcgatgta gccgaagacc acggtttcga tcagcagcaa
     3181 ggtgatatgg aagaggtcgg cccattgagg cacaccgtta aggaagtgcc ggaaggtcgc
     3241 aataatctgg gcgggcggcg ttgcccaggc aactacctgt agccagcccg gcaaccgttc
     3301 aatcggaaaa aacgtgcctg acagccagaa cagcgggata atcacgacgt taaagtagta
     3361 gccgagaaag ctgatcgcgg gcgccatcgc cgtaaagcac agcgtgagcg ccccgaaaca
     3421 gaggccgagc aggtaggcga cggggagcac cagtacggcc atcggcgact gcacgatgcc
     3481 gtaagttggc gtcaaggcga gcaagaccgc caggatgtaa aagctgttga tcaccgagcg
     3541 ggtagccgcc cacattactt cccccgccac gatatcgtcg agcgtcgccg gcgtggcggc
     3601 gactgcggtg taggttccct gattttccat gcggaaatat gcgccccagc cgcactcaaa
     3661 cgttgcctgg aacataccga actgcgcgag gatgcccggc gccaggaacg ccacataggt
     3721 gcccgatccc ccgagactta tgaagtgacc gaggccgatg cccatcgccg tcagaaccag
     3781 aatgggctcg acgagcggca tgatgagctc ggtcttccac agtttgaggt agacgtcgcg
     3841 gttacgccgc cacacgttta acgcgtggtg cgataagtcg ggcaggttga agttgtgcga
     3901 tcggagtgcc cgttgcgtgg cgatcaatcg agtaaccccc tgcctgtcag ccgcaggaag
     3961 acgtcctcca ggttggcacg gcgcacaaag atatcgtcgg ccgcgtcttc gaagcgcatc
     4021 gccagcgacc caaccggtac gtcacagaac aggtagatcg tgtcgccggt gtcttcgatc
     4081 tggagcccct caaccattgc ggcggctgcc aggatctcgt tggtgcgacc tggggccggt
     4141 cgaagctcaa tgacttcgtt gccggcgtgc tcgcgcacca gcgcctccgg cgtgccctgc
     4201 acgagaatgc ggccctggtg catgattacc agccgatcgc aaaggcgcgc cgcctcgtcc
     4261 atgtagtgcg acgtcaacac catcgtcatg ccttgatcct taagagcgcg tagctgacgc
     4321 cacaccaggt ggcgagcctg cggatcgagc cccgttgtcg gctcgtcgag cacgagcaag
     4381 gttggagcgc ttatcaaggc acgggcgatg gtcaaccgtc gcttgaggcc gccggatagc
     4441 gcgttgattt tttcgttccg cttgtcggtg agctggaaca ggtcgagaac ttcgtcggcg
     4501 cgggccgctg ccacggcgcg cgagatgtca aagtagcgcg cgtagacaag aaggttctca
     4561 cgcaccgtca ggtcgccatc caggttgtcg tcctgagaaa cgacacccag gcgcgctttg
     4621 atacggcgat gatcggagcg aacatcgagg ccgtctacca gtagttcacc gccgcttggc
     4681 ggcgacatac acgtggtcat gcgcatcgtg ctggttttcc cggcgccatt tggaccgagg
     4741 aaaccgaagc actcgccggg taggacgtcg aaatcgatat ggtctacggc ggtgaagtca
     4801 ccgtagcatt tgtagagaga gcgggctgtg atgagcgctt gcactgaatg gtcctgccgg
     4861 tgtttctgca ccggcggcac cggtgcgggt tattcgtctt cgaacgtgcc gtcgtcgcgt
     4921 acaacgcctt cgtgtgcttt ttggtactcg agcagggtca cgtccccctg accgtgggcc
     4981 tcaccgtcgg tctcccagcc ttcgcccgtt tcgacctctt cgagaatttc ctcgtcctcg
     5041 tcgtcgtaac ggatgagccc ttcccgggtg tacggccgag gtgtcccgtc tactcctgcg
     5101 ggcgggaacg acagacggcc agaattggct gcggactgga gcgtctcctt gatgatgatg
     5161 cgcgcggtat cgctgcccag ttgcgagatt gccgccgcgt attcattgcc gcccttcatc
     5221 agattaatca gccgcagggc gattcgcggt tcaatctgtc cgagtgcatc gcccgccgcg
     5281 ttcgtagcga gcaggaggtt gccgtcgggc cgcaaatgaa cacgttcgcc tggagtcatg
     5341 tgcgacagcg cgtctttacg gggccgctga agcaccgtga gtcctgtctt gcctgtttct
     5401 tcgacaaaga gcttcggatc gactttgtct cgcggagccg cgccggcgtc ggattcgatc
     5461 tcagctttct tttccagtgc ggcgatgttt ttcttagcga tctggttggt cgggtccagc
     5521 ttcagtgccc ggccataggc ttcacgcgct tccttatatt tgccgagtgc catgagggcg
     5581 cggccaagcc tgttgaacgc atcgccgtca cgctggccgg tcatttcgag aacggacagg
     5641 ttaaccgtaa ccgcttcttc ccagcggctt tgcatggcaa gacgaatcgc ttgctcagtg
     5701 cgctgcttgc ggaggcggct gagctcgtct gtccgataga ccatagaccg gcaccttaac
     5761 cgtaggctcc gcgcattgta tgcgctgata tttatg
//
LOCUS       DATCPE010000041         6141 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U7219
            k141_71185, whole genome shotgun sequence.
ACCESSION   DATCPE010000041 DATCPE010000000
VERSION     DATCPE010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955033
            Sequence Read Archive: SRR1586279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 6141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 16:49:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 640
            CDSs (total)                      :: 631
            Genes (coding)                    :: 627
            CDSs (with protein)               :: 627
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6141
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71185"
                     /isolate="SMAG_U7219"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(201..3053)
                     /locus_tag="VIM45_01255"
     CDS             complement(201..3053)
                     /locus_tag="VIM45_01255"
                     /inference="COORDINATES: protein motif:HMM:NF024703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HEY8819946.1"
                     /translation="MNNQPASWEWFVSLDLEMTGVDPETDEIIEIGAVRFAPDGRTER
                     FQSLVNPGRTLSYRIQTLTGIHDEELQSAPPLAVVSGALRAFLGNYPIVGQHVAFDLL
                     YLSKHGIRPTGPVFNTAEIAELLSPGDAEYSLRALTRRLCVDFPVQHRALGDAEAAME
                     VFRKLRDHAADIDPLILNEVVQLTADSSWPLRFFFSDVERSSPRPVPERPDSGIVLDL
                     RAPDKSVMRALTPVADRVEVSPEEVGRIFDEVQRQPAVYNGFEQRPQQIEMAQAVAVA
                     LNQDTNLLVEAGTGTGKSLAYLLPAACFALRNNERVVVSTSTISLQEQIIGKDVPAML
                     RVLETCGPEDIRQALHDFRAVPLKGRGNYICLQKFATFRRQSLLTSDEARFAAKLLLW
                     LLKTETGDRSELNLRGDEENLWPRVSAANVNCFAGPTYYVRNGSCQLMRARKRAENAH
                     LVVVNHALLLSDIAAGGRVLPTYDRIIVDEAHNLEDEATSQFGFRTGQTELRNALSAI
                     YDRTAEREHGLVADIRLALRSDDDPLQQEKFIRGLLDAVAGQVDRARERVPEIFGRLH
                     AFAATHGESGGDYDNRLLITAGKRAQPEWEHVEIAWDNLRIALDAVNDGLSRLLVALS
                     ETSSGIAGHENLASQLQTQSLDLARLTRGVDAVILRHDEERIAWLTVNRQFGTTSLAS
                     APLNVGEVLESYLFGRKSSVILTSATLSTAGNFKYVRNRLGLEEVDELALGSPFDYKR
                     SALVLMPEDMPEPNRLDYQPALESAIVDLVRASAGRALVLFTSHSALRTAYYAIKARL
                     TGDGIRVMAQGIDGAPDDLLEALRTDRKTVLLGTSSFWEGVDVAGEALSLLIIAKLPF
                     SVPSDPVFAARSELFTDPFKDFALPQSVLRFKQGFGRLIRHRNDRGVLVVLDRRVRSK
                     AYGRAFLQSLPDCSSAEAPLGQLSSLVRQWLKTPQA"
     gene            complement(3110..3823)
                     /locus_tag="VIM45_01260"
     CDS             complement(3110..3823)
                     /locus_tag="VIM45_01260"
                     /inference="COORDINATES: protein motif:HMM:NF024660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP-associated domain-containing protein"
                     /protein_id="HEY8819947.1"
                     /translation="MTGTIVDMHCHTTNGASDSALTPAELIEVSQRIGMTGVNVSEHD
                     RVWDRYTVAKFREDSSMFVCPGMEVSTDLGHMITIGLTEYLPGIRHCAELRKALDKVG
                     GFLIVAHPFRHWFDPVTFRRTGKEPPVMTVENMAKLEVFQLVDGVEVLNGANTARENR
                     FALEVANYIGKPGTGGSDAHSTSGVGIYTTVFEKNLTTVEEMLEELHARRFKASLGLP
                     EGKLRPFTLDEFVTPESIR"
     gene            complement(3846..4784)
                     /gene="ispE"
                     /locus_tag="VIM45_01265"
     CDS             complement(3846..4784)
                     /gene="ispE"
                     /locus_tag="VIM45_01265"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEY8819948.1"
                     /translation="MSAALRLRAFAKINWSLEILGRRWDGYHEVRTVLQTIDWWDDIC
                     LVPGHLPVLEVVPMAGNSVPDGAENLAERARALYTGAGDAMQPLSIRLGKRVPTAAGL
                     GGGSSDAATVLRGLDRLSDRPVGAGRLEQLASDLGSDVPFFIHGGTQVATGRGEVLER
                     LGGAPAAWLVVLTPNIAVERKTARMYSLLGSADYSNGDSVAKIAEALSAGALPDANTL
                     KNTFDRMSEQAYPGIWRYRRALVDTRGHALLCGSGPALFALCGSAAEAEAAALRLRRL
                     GMPARAVRTVTAAESTAVEYVNEAAQPDIVKERTGE"
     gene            complement(4781..5713)
                     /gene="rsmA"
                     /locus_tag="VIM45_01270"
     CDS             complement(4781..5713)
                     /gene="rsmA"
                     /locus_tag="VIM45_01270"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEY8819949.1"
                     /translation="MPRRNQPPARERFRPGSSRQRGRTSPAHNRPRIGQHFLKDPLIA
                     HDIVDAAGLTLDDDVLEVGPGGGAITGLLLERAAHVTAVELDDNLAEALRRKYAGESR
                     LTVIAASILAHPVDQLLAEGGRKPPYVVVANLPFYITAPVMRYFLEEGPRPRRFIVMV
                     QREVAESIAGQRKGLSLLGVSVQVFASVEVLFRVPPRAFDPPPKVDSAVIRLDVYSEP
                     LIPEQDLPAFFEVVRAGFRNPRKQMHNALSGGLWMPPGDAPGLLEAAEIDPIRRAGTL
                     KIAEWRRLQLAYAAARAEFGPHPSIRSDTKRDVT"
     gene            complement(5785..6050)
                     /gene="ffs"
                     /locus_tag="VIM45_01275"
     ncRNA           complement(5785..6050)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VIM45_01275"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
BASE COUNT         1175 a         1949 c         1840 g         1177 t
ORIGIN      
        1 cagagaaggc gttccgccgc tgctaccgcc caaccactct ctcaaagacg gagattcgtg
       61 gcgcccgctt tcgccgggtc cgaatatctc gggccaagtc gaagagaacc aggattcgag
      121 gccgtcagcg gattccgagg ccggctgaac ggtcaccctg agccccggta gcggtccgaa
      181 catttccgtt tgcgcgtctc ttaagcctgg ggcgttttca gccattggcg gactagcgaa
      241 gacagttgcc ccagcggcgc ctcggcgctg ctgcaatcgg gcagcgattg caagaacgcg
      301 cggccgtatg ccttgctacg cacgcggcgg tctagcacca ccaggacacc gcggtcattg
      361 cgatgacgga tcagccgccc gaacccctgc ttgaagcgaa ggaccgactg tggcagggcg
      421 aagtccttga acggatcggt aaacagctcg cttcgagcgg cgaataccgg atcgctcggc
      481 accgaaaagg gcagctttgc gatgatcaga agtgaaaggg cctcgccggc cacgtccact
      541 ccttcccaga agctcgacgt acccagcagc accgttttac gatcggttct cagcgcttcc
      601 agaaggtcat cgggggctcc gtctatgccc tgggccatta cgcgtatccc gtcccccgtt
      661 agccgcgcct tgatcgcgta gtaggccgtc cgcaaggcgc tgtgagaggt gaacagaacg
      721 agcgcacgcc cggcgctggc gcgtacgaga tctacgatcg cggattccag ggccggctgg
      781 tagtcgagcc ggttcggttc gggcatgtct tctggcatca gaacgagcgc tgatcgcttg
      841 taatcgaatg gggaaccgag cgccagttca tcgacctctt ccaggccgag acggtttcgg
      901 acgtacttga agttacccgc cgtgctgagc gttgcgctgg tgaggataac gctcgacttc
      961 cttccgaaaa ggtagctttc caacacctca ccgacgttca gcggcgccga ggcaaggctc
     1021 gtcgtaccaa actgccggtt aaccgtcagc caggcgatgc gctcctcgtc gtggcgcagg
     1081 atcaccgcat caacgcccct ggtcagacga gccaggtcca gcgattgtgt ctgtagctgg
     1141 gaggccaggt tctcgtgccc ggcgatgcca ctgctggtct cgctcaacgc aacgagcagg
     1201 cgggacagac cgtcattgac cgcgtcaagg gcaatccgca gattgtccca cgcgatttcg
     1261 acatgttccc actccggctg ggcacgcttg ccggcggtga tcaagaggcg gttatcgtag
     1321 tcgccgcccg attcaccgtg cgtagccgca aatgcatgca gacgtccgaa tatttccgga
     1381 acacgttccc tggctcggtc gacctggccg gccaccgcgt ccaacaaacc ccggatgaat
     1441 ttctcctgct ggagcgggtc atcatcggag cggagagcca accggatgtc tgcgacaagg
     1501 ccatgctctc gttcagccgt gcgatcatag atggcggaca gggcgttgcg aagctcggtc
     1561 tggcccgttc taaagccgaa ctgactcgtt gcttcgtctt cgaggttatg tgcctcgtcg
     1621 acaattatgc gatcgtacgt tggcagaacc cgaccacccg ccgcgatatc ggacaacagc
     1681 aatgcgtgat tgaccaccac gaggtgggcg ttttcggcgc gtttccgcgc ccgcatcaac
     1741 tggcaggagc cgttgcgcac gtagtacgtc gggcccgcga aacagttcac gtttgcggcc
     1801 gacacccgcg gccacaagtt ctcttcatca ccgcgaagat tgagctcaga tcggtcgccg
     1861 gtctcggtct tcagcaacca gagcagcagc ttggcggcga atcgcgcttc gtcgctggtc
     1921 aataagctct ggcggcgaaa cgtcgcgaac ttctgcaagc agatgtaatt tccgcgcccc
     1981 ttcagcggca cggcgcggaa atcgtggagg gcctgccgaa tgtcttcagg gccgcacgtt
     2041 tcgagcacgc gcagcatggc tggcacgtct ttgccgatga tctgctcttg cagactaatg
     2101 gtgcttgtag acacgacgac ccgttcgtta ttgcgaagcg caaagcaggc ggccggcaac
     2161 aggtaggcaa gggacttgcc agtaccggtt ccagcttcca ccaaaaggtt cgtgtcctga
     2221 ttcagcgcaa ctgcgaccgc ctgcgccatt tctatttgtt gaggacgctg ctcgaagccg
     2281 ttgtagaccg caggttggcg ctgaacttca tcgaaaatgc ggccgacttc ttccggcgat
     2341 acttcgaccc tgtcggcaac aggagtcagc gcgcgcatca cggacttgtc cggggctctc
     2401 aggtcgagaa cgatgccgct gtcaggccgc tccggaactg gtcgaggaga tgagcgttca
     2461 acgtcgctga aaaagaatcg cagcggccaa gacgagtccg ccgtaagttg gacgacctca
     2521 ttgaggatca gtgggtcaat gtcggcggcg tggtcccgga gctttctgaa gacctccatt
     2581 gccgcttcgg cgtctccaag ggcgcgatgc tgaaccggaa agtcgacgca cagcctccgc
     2641 gtcagcgcgc gaaggctgta ctcggcgtct cctggcgaaa ggagctcggc tatctccgct
     2701 gtgttgaaca cggggccggt tggccggata ccgtgcttgg agagatataa caggtcgaag
     2761 gcgacgtgtt ggcccacgat cggatagttg ccgaggaacg cccgcagcgc gccagatacg
     2821 acggcgagcg gcggtgctga ctgtagctct tcatcgtgga tccctgtcag cgtttgtatt
     2881 cggtacgaga gcgtccgtcc gggatttacg agcgactgga agcgctcagt gcgcccgtcc
     2941 ggggcaaagc gcacggcgcc aatctctata atctcgtctg tttccgggtc gactcccgtc
     3001 atttcaaggt caagggagac gaaccattcc caggacgcgg gctggttatt caaataatac
     3061 cggcaatctg gcagcccttc agctgaaact gtgccgctga tgcggcgtgc taacggatac
     3121 tttcgggagt cacgaattca tccaaagtaa agggtcgaag ctttccctcg gggaggccaa
     3181 gactcgcctt gaatcgccgg gcatgaagct cttccagcat ctcctcaaca gtggtcaggt
     3241 tcttctcgaa aaccgtcgtg tagatgccca cgccgctggt cgaatgcgcg tcgctacccc
     3301 ctgtgcccgg ttttccgatg tagttagcca cttccaatgc aaatcgattc tcgcgggcgg
     3361 tgttggcgcc gttgaggact tcgacgccgt cgacgagttg gaatacttcc agtttggcca
     3421 tattctcaac cgtcatcacc ggcggctctt tccccgtgcg gcgaaaggta accgggtcaa
     3481 accaatgtcg gaaagggtgg gccacgatca ggaagccgcc gactttatcg agcgccttcc
     3541 ggagctccgc gcaatgccgg ataccgggca agtattcggt caacccgatc gttatcatgt
     3601 gaccaaggtc ggtcgacacc tccatcccgg ggcacacgaa catgctgctg tcttcgcgaa
     3661 atttggccac cgtgtaccgg tcccagacgc ggtcgtgttc gctgacattc acgcctgtca
     3721 tgccgatgcg ctgggatacc tcaatgagct ccgccggggt gagggcactg tccgaggcac
     3781 cgttggtagt atggcagtgc atgtcgacga ttgtgcccgt catccccacc tcagcagcgc
     3841 cgcgctcatt cgcctgtacg ctccttcact atatctggct gagcggcctc gttcacgtat
     3901 tcgacggcgg tgctctccgc agcggtaacc gtccgaactg ccctcgccgg cattccgagg
     3961 cggcgtaatc gtaatgcggc ggcttcggcc tcagccgcgg aaccgcagag cgcgaacagc
     4021 gcgggtcccg agccgcacaa aagcgcgtgc ccgcgcgtat cgacgagtgc tcgacggtat
     4081 cgccagatac ccgggtacgc ctgctccgac atgcggtcga acgtattctt cagcgtgttc
     4141 gcgtccggta gcgcacccgc ggatagggct tccgcgattt tggcaactga gtcgccgttg
     4201 ctgtagtccg cactaccgag caacgagtac atccgcgccg tcttgcgttc gacggctatg
     4261 ttgggcgtca ggacaacaag ccacgcagcc ggcgcgcctc ccagacgctc cagcacctcc
     4321 ccgcgcccgg ttgcaacctg cgttccacca tgaataaaga acggcacatc cgaaccaagg
     4381 tcgctcgcca gctgctccaa acggccggca ccgaccggcc gatccgatag gcggtccaga
     4441 ccacgcagta cggttgccgc gtcgctgctg cctccgccca ggcccgcagc ggtaggcacc
     4501 cgcttcccga gccgaatcga caacggttgc atggcgtctc cggcccccgt gtagagcgcc
     4561 cgcgcccgtt cagccaggtt ttcagcaccg tcgggaaccg aattgcctgc catcgggacg
     4621 acttctaaga ctggaagatg tccaggaacc aggcagatgt catcccacca gtcgatcgtt
     4681 tgcagaactg ttcgtacttc gtgatagccg tcccaccggc gaccgagtat ttcaagcgac
     4741 caattgatct tggcaaaggc ccgtagacgc agagcggcgc tcacgtaacg tctcgcttgg
     4801 tatcgctccg gatagacgga tgcggtccaa actccgccct agccgccgca tatgcgagct
     4861 gcaacctgcg ccactcggca attttcagcg taccagcgcg gcgtatagga tcgatttccg
     4921 ccgcttcgag tagacccgga gcgtcgccgg gtggcatcca caggccgccg gacagtgcat
     4981 tatgcatctg tttgcgaggg tttcggaatc cggcccgcac gacctcaaaa aacgccggca
     5041 gatcctgctc tgggattagc ggctcgctgt atacgtccaa ccggataacg gccgaatcga
     5101 ccttcggcgg cggatcgaac gcccgcggcg gcacgcgaaa cagcacttcc actgaggcga
     5161 agacctgcac cgacaccccg agcaggctca gcccctttcg ctgtccggcg atactctccg
     5221 ctacttctcg ctgaaccatg acgatgaagc ggcgcggacg cggcccttcc tccagaaaat
     5281 atcgcatgac gggtgcggtg atgtagaacg gcaggttggc gacgacgacg tacggcggct
     5341 ttctgcctcc ctcagcaagg agctgatcta ccggatgggc gagaatgctc gcggcgatca
     5401 cggtgagacg cgactcaccc gcatatttgc gccgcaatgc ctcggcgagg ttgtcgtcca
     5461 actcaactgc cgttacatgt gcggcgcgct ccaggagtaa gccggtgatc gcgcccccac
     5521 ccggccccac ttcgagaacg tcgtcgtcca gtgttagacc ggcagcgtcg acaatgtcgt
     5581 gcgcgatcaa cgggtctttg agaaaatgtt gccctatccg ggggcgattg tgggccggcg
     5641 aggttctccc ccgctgccgc gatgagccgg gccggaaccg ttcacgagct gggggttggt
     5701 tgcgacgcgg cacctgttcg gccctttaaa gcaaagggcg gacggctgaa ttcagcgtcc
     5761 gcccgtaacc tgtcgggagt caggtcgtgc acccattgtc gatctcgccc cgcgagctta
     5821 ccttcgccgt ccggctcggg ccacgcaccc cggcggcacc cgaaagggcc cacttacggc
     5881 tgctgtcttc cgaccctgac cgggttcatg gtctttcgcc gcgccggacg cagcctgtca
     5941 acaccggcag cgtcggcagt cctctgggac cgcagacctc gaatgggagt tcagccccgc
     6001 ctaaggaggg tttcaggtac agggcacccc caacaccccg cttagcacga ccacatatat
     6061 gatagcgact gcgcaggcgg gggacaacgc gattagacgt gagtgcgtac cagcgccccg
     6121 tcgtcgagca ccgtaacgcg c
//
LOCUS       DATCPE010000042         8203 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U7219
            k141_493565, whole genome shotgun sequence.
ACCESSION   DATCPE010000042 DATCPE010000000
VERSION     DATCPE010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955033
            Sequence Read Archive: SRR1586279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 8203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 16:49:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 640
            CDSs (total)                      :: 631
            Genes (coding)                    :: 627
            CDSs (with protein)               :: 627
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8203
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_493565"
                     /isolate="SMAG_U7219"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..352)
                     /locus_tag="VIM45_01280"
     CDS             complement(<1..352)
                     /locus_tag="VIM45_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010937019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HEY8819950.1"
                     /translation="MEIIPAVDIRGGKCVQLAQGDYARERVYNADPVAAALEWQSLGA
                     TRLHVVDLDGARDGKPVNRPVVERIVAALKIPVEAGGGIRSVEDALGYAHAGVDRVIL
                     GTAAVEDVVLVQSLA"
     gene            complement(352..981)
                     /gene="hisH"
                     /locus_tag="VIM45_01285"
     CDS             complement(352..981)
                     /gene="hisH"
                     /locus_tag="VIM45_01285"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEY8819951.1"
                     /translation="MELVIVDYGAGNLRSVARAVEKTGRRPLVSCRPEDVVAAKALIL
                     PGVGAAADTMRNLTSSGLVEPVKEYIASGRPFLGVCMGFQALMTLSEEGGEHPCLDVL
                     RGRVRRLEGGDGLKVPHMGWNNVRQLRPHPIFRGIPNDAYFYFVHSYFVDPEDECLTI
                     GQAEYGRTFTAVAARDNLVATQFHPEKSGIHGLMVYANFLDNALGAGAK"
     gene            1097..3124
                     /gene="ligA"
                     /locus_tag="VIM45_01290"
     CDS             1097..3124
                     /gene="ligA"
                     /locus_tag="VIM45_01290"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEY8819952.1"
                     /translation="MRVEELRSQINQANYRYYVLDEPQISDANWDAMLRELRELEAQF
                     PNLVTPDSPTQRVGAQPLTEFAPVRHRRPMLSLANAFNPDELRRWYQRACRLAEVDCL
                     ELVCELKIDGLAIALVYENGTLTQGATRGDGLTGEDITQNLRTVRSIPLTLDHELAPP
                     IFEVRGECYMTRTGFERLNERRASAGDRLFANPRNSAAGSLRQLDPSITASRPLHTFM
                     YQLGWVDGSETPSTHWEILQWLSRLGFRTNPNIRLVPDIEAAVAFCDEWDSKRPQLDY
                     PIDGIVVKVNSLLMQQHLGTVGRDPRWAIAYKYPSAEETTKLVDIGVNVGRTGTLNPY
                     AILEPVQIGGVTVALATLHNEEDIHRKDIRIGDTVIVHRAGEVIPQVIGPVVSLRTGA
                     EQPWQMPENCPVCGTPVVHPLGEAMCYCPNRACPAQAFRLMVHFVGRNAMDVEGIGES
                     LAQQLLEACVVNDPGDLYSLTKEQLAGLDRMGEKSAQNVINEIEASKQRPFGNVLFAL
                     GIRHVGEETARLLAEHFGGIDALKSATDADLESVPSIGPKLAQSIEAFFQDERNLQLV
                     EKLRAADVRLKADGTPRSEGPLSGTTWVVTGKLDAMPRGAAETRLKDLGAKVSSGVSK
                     TTSFVVVGADAAGHSKFKSAQKLGVPLLDEEAFLRVLRDPAALPRFADTVK"
     gene            3311..4375
                     /locus_tag="VIM45_01295"
     CDS             3311..4375
                     /locus_tag="VIM45_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEY8819953.1"
                     /translation="MAFNPLSALFGLFSHDVGIDLGTANTLVMVKGKGIVIDEPSVVA
                     IDRNTKKILAIGAEAKRMVGRTPANIVAVRPLKDGVISDFEVTEKMLQYFIRAVHDRF
                     GFGFPRPRVVVGIPSGVTEVEKRAVHDAALNAGARFAYLIEEPMAAAIGSGLPIMEAN
                     GTMIVDIGGGTTEVAVIALGGIVVSTSIRTAGDEMDQDIIAYARQIHNMLIGERTAED
                     IKVQAGSAYAQDPETSVQLRGRDLATGLPKSVDVSSVEIRDALSGSINAIVDAVRSTI
                     EITPPELISDLMAHGIALAGGGALLKGLDRRLSQETRFPVYVADDPLTCVVRGTGEVL
                     EEAAMLAKVQSRLAQRRPPR"
     gene            4458..5288
                     /gene="mreC"
                     /locus_tag="VIM45_01300"
     CDS             4458..5288
                     /gene="mreC"
                     /locus_tag="VIM45_01300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEY8819954.1"
                     /translation="MSLSRPLTWTLSLLLLGVVALGLSRTGATDPLQSLAVRVFSPLD
                     SGVHSVFTPIADFVNNVGSYGDLRKENRDLRADNERLNAAVNQLREQQAEAGQVDALT
                     QSTKLFPDADFVNANVIARDPSNVHDAVLIDKGKNDGVLSGMVIMGKEGSLLGTVSKA
                     LSTSAWVRLTTDPDSDVNAEVQETRAMAIVSGAFGHKLQLQFVAEGTDVKPGDTVVTS
                     GLGGNYPKSLLIGRVSKVEGGPTELFKTIQVDPAVRPGTLESVIVMTSFVPSRVDTGQ
                     "
     gene            5285..5791
                     /locus_tag="VIM45_01305"
     CDS             5285..5791
                     /locus_tag="VIM45_01305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8819955.1"
                     /translation="MIYYAAIPLSIFIVVLQVAAAPSFTYLGVHADLPVVWLGCWAAA
                     RGRREMLPLVGVTGLALGLLGREPLGASVLALLPLAGLAVLDESPAIVGRFVTALLVV
                     FVGGVMFAVIHALTSFAGGEGFGSPLNVLRIAPRAAVLDVMTAALWYWPVRLFFGRHT
                     RAGEFRRM"
     gene            5842..7851
                     /gene="mrdA"
                     /locus_tag="VIM45_01310"
     CDS             5842..7851
                     /gene="mrdA"
                     /locus_tag="VIM45_01310"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEY8819956.1"
                     /translation="MGLFSRERRWRAESRTPRTEEDLSRSSNRRLIVMRLVVVLLFCA
                     LGLQLLRFQVWSGGRYQLKAESNRLRLTVTESPRGLIYDRHHNPLVANIASYTAIVIP
                     ADIPKGADRDTFYQLSGVIGVPPDVIEQKVTDSIKRTDPFTPVPIKEGLDQRTVLTLA
                     ELRHTFPGVDVKYDAVRQYTNSTLMAHIYGYVGKVAEDEYDQLKDRGYQLSDKIGKTG
                     VEYAYEDQLRGTLGKQQVEVDASGRVLRTLDGIPPKPGNSLVLSIDLDLQRHVTDILQ
                     QSLIQYGSPSGAAVMMDIHTGEILAYVSLPSYDVNLFSKPISNKDYQALLDDPGKPLV
                     DHISDDQYPPGSIFKTITGSAALETGVASLNTTLTSHGAFSVTNNPNDKANVVYDWAD
                     LGTLDFLHGLSMSSDIYFYCLAGGCPLLPGGGISTNSALGADRLAKFARDFGLGSKTG
                     IDLPDEASGLIPTTDWKAKTQKDEKGNPLPWVVADTYFMGIGQGYDTATPIQMVRLAA
                     TIANGGDLLRPHVVHEIDDPAGNVVVPPQREVVRHVGVSDQNLAIIRQGMLMAVEGGS
                     AQNGFLPGYHIAGKTGTAEFGVAIQPAGDSNNGTYNEHGWYMSYAPYNNPEVALVVFH
                     ERGGGAVSAAPTAQKIWDYYFNHYKAGDKTPELKASPTAAAGIGH"
     gene            7848..>8203
                     /locus_tag="VIM45_01315"
     CDS             7848..>8203
                     /locus_tag="VIM45_01315"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEY8819957.1"
                     /translation="MIRDLRRFDYPMLLSCLLLVGYGLALIYSGSLALYGTSHAALTH
                     AVFKQAAFAVVGLLLLGLLTQIDYRVWSNLAPILYGLTIVGLLAVLVIGQRAFGSRRW
                     FSLAALQLQPSELAKLV"
BASE COUNT         1597 a         2552 c         2492 g         1562 t
ORIGIN      
        1 ccgcgagact ctggaccagc accacgtctt cgaccgccgc ggtgccgaga atgactctgt
       61 ctactccggc atgcgcataa ccgagggcgt cttcgacgct gcggatgcct cccccggcct
      121 ctacgggtat tttaagtgcg gcaacaatgc gctcaactac tggccggttc accggtttgc
      181 cgtcacgggc gccgtcaagg tcgaccacat gcagccgcgt cgcacccagg ctctgccatt
      241 ccagcgccgc cgcgacggga tcggcgttat aaacgcgctc gcgagcgtag tcgccttgcg
      301 ccagttgaac gcactttccg ccgcgaatat cgaccgcggg gatgatctcc actacttggc
      361 gcccgcgcct agggcattat cgaggaagtt cgcatagacc atcaggccgt gtatgccgct
      421 cttttcaggg tggaattgcg tggcgacgag attgtcgcgc gctgcgacag ccgtgaatgt
      481 ccgaccgtat tctgcctgcc cgatcgtcaa acactcgtcc tctgggtcca cgaagtagga
      541 atgtacaaag tagaagtacg catcgttcgg aatgccacgg aagatcggat gcggccgaag
      601 ctgccggacg ttattccagc ccatgtgcgg cacctttaat ccgtcgccgc cttcgaggcg
      661 ccgcacgcgc ccgcgcagaa cgtcaaggca ggggtgctct ccaccctctt cgctgagcgt
      721 catcagggct tgaaagccca tgcaaacgcc gaggaatggg cggccgctcg cgatatattc
      781 cttgacgggc tcaaccaggc cgctggacgt gaggttgcgc attgtgtctg ccgcggcgcc
      841 aacgcccgga agaatcagcg ccttggccgc gacgacgtct tcggggcgac agctcacgag
      901 cggccgccgg ccggtcttct caaccgcccg ggcgacgctg cgcagattgc ctgcgccgta
      961 atcgacgatc acgagttcca cgcaatgctc ccgaagctga ttgtagcatg agaattgttt
     1021 gtttttgaat tacaccagcg atggctctta tacttctgca atgacgaacg aggaacgtgc
     1081 ccaccaggcc gccgcgatgc gggtcgaaga gctgcgctcc cagatcaacc aggcaaacta
     1141 caggtattac gtcctcgatg agccacagat tagcgacgcg aactgggacg cgatgctccg
     1201 tgaattgcgc gagctcgaag cgcaatttcc gaacctcgta acgccggatt cgccgacgca
     1261 gcgggtgggt gcccaaccgc tgaccgagtt tgcgccggtg cgacaccggc ggccgatgct
     1321 gagccttgcg aacgcgttca atcccgacga gttgcggcgc tggtatcagc gggcgtgcag
     1381 gttggcggaa gtcgactgcc tcgagctcgt ctgcgaattg aagatcgacg ggctggcgat
     1441 cgcccttgtc tacgagaatg gcaccctcac acagggcgct acacggggcg atggactgac
     1501 gggcgaagat atcacccaga acctgcgaac ggtgcgaagc ataccactca cgctggacca
     1561 cgaactggcg ccgccgatct tcgaagtgcg cggcgagtgc tacatgacgc gcaccggctt
     1621 cgagcggctg aacgaacggc gggcctcagc gggcgaccga ctgttcgcga acccgcggaa
     1681 ctcggccgcg ggctcgcttc gccagctcga tccgtcgatc accgcctcaa ggccgctgca
     1741 cacgttcatg tatcaacttg gttgggtaga tggctccgag accccgtcaa cacattggga
     1801 gatcttgcaa tggctgagtc ggctcggttt ccgtacaaat ccgaacatca ggctcgttcc
     1861 cgacatcgaa gccgcggtcg ctttctgcga cgaatgggac tccaaacggc cacagcttga
     1921 ttatccgatc gacggcatcg tcgtcaaagt aaattcgctt ctgatgcagc agcatctcgg
     1981 aacggttggc cgggacccgc gctgggcgat cgcgtacaag tatccgtcgg ctgaagaaac
     2041 gacaaagctg gtcgacatcg gcgtaaacgt cggccgcacc ggcacgctga acccgtacgc
     2101 catcctcgag ccggtgcaga tcggcggtgt gaccgttgcg ctggctaccc tgcacaacga
     2161 agaggatatt caccgcaaag acatccggat cggcgacacg gtgatcgtcc atcgagccgg
     2221 cgaggtcatc ccccaggtca tcggaccggt cgtttcgcta cgcaccggag cggaacagcc
     2281 gtggcagatg ccggaaaact gcccggtatg cgggactccg gtcgtgcacc cgctcggcga
     2341 agcgatgtgc tactgtccca accgggcgtg tcccgcccag gcgtttcggt tgatggtgca
     2401 cttcgtcggc cgcaacgcga tggatgtcga ggggatcggc gaatcgcttg cgcaacagct
     2461 tttggaagcc tgcgtcgtta acgacccggg cgatctctac tcactcacaa aggagcagct
     2521 cgcgggtctc gaccggatgg gcgagaagag cgcccagaac gtcatcaatg agatcgaggc
     2581 gagcaagcaa cgaccgttcg gcaacgtgct gttcgctctc ggaatccggc acgtcggcga
     2641 agagacggcc aggctgttgg cagagcattt tggcggaatc gacgcgctca agtcggcaac
     2701 tgacgccgat ttggaaagcg taccttccat cggcccaaag cttgcccaga gcatcgaagc
     2761 gttctttcag gacgaacgca atttgcagtt ggtcgaaaag ctgcgcgccg cagacgtgcg
     2821 gctcaaagca gacggcacac cccgctccga agggccgctc agcggcacaa cctgggtggt
     2881 gaccggcaag cttgacgcca tgccgcgagg ggcggccgag acccggctga aggacctcgg
     2941 tgcgaaggta tcctccggtg tctcgaaaac caccagcttc gtcgtcgtcg gagccgatgc
     3001 ggcgggtcac tccaaattca aaagcgccca gaaacttggg gtgccgcttc tggacgaaga
     3061 ggcttttctg cgtgtcctgc gtgacccagc cgcactgcca cggttcgcag ataccgtcaa
     3121 atagcggcag gttacgggtt aacttgtgcc aattttcgga ttcggcggcg gacatccata
     3181 tgccgaagcg gcaaaatgtt aattcgcttt cgcgctttgc cgcctcaggg ggtttcgtta
     3241 tactttcagg ggctccagga cgccggaccg ccgccgcagc gcatacggac cgcgaaggag
     3301 aatactgcca ttggccttta atcccctgtc ggcgctcttc gggcttttct cgcacgatgt
     3361 tggcatcgat ctcgggaccg ctaatacgct ggtcatggtg aagggaaagg gcatcgtcat
     3421 cgacgagccc tcggtcgttg cgatcgaccg taacaccaag aagatcctcg caatcggcgc
     3481 agaagcaaaa cggatggtgg gacgcacgcc cgccaacatc gtcgcggtcc gcccgctcaa
     3541 ggacggggtg atctcggact tcgaggtcac agagaagatg ctgcagtact tcatccgcgc
     3601 ggtgcacgac cgtttcgggt ttggctttcc acgcccgcgg gtggtggtcg gcatccccag
     3661 tggcgtgacc gaagtcgaaa agcgcgccgt acacgacgcc gcccttaacg ccggcgcccg
     3721 ctttgcctac ttgatcgaag agccgatggc ggccgcgatc ggctcgggcc tgccaatcat
     3781 ggaagcgaac ggtacaatga tcgtcgatat cggcggcggc accaccgaag tcgcggtcat
     3841 cgcgctcggc ggcattgtcg tcagtacctc gattcggacg gcgggtgacg agatggacca
     3901 ggatatcatc gcctatgccc gccagattca taacatgctg atcggcgaaa gaacggccga
     3961 ggatatcaaa gtacaagccg gctcggcgta cgcgcaggat ccggagacgt cggtccagtt
     4021 gcgcggtcga gacctcgcga ccggtctgcc aaagtcggtc gacgtcagca gcgtcgagat
     4081 ccgcgacgcc ttatccggct cgatcaatgc catagtcgac gcggtccgat cgacgatcga
     4141 gattacgccg cccgagctta tctccgacct gatggcgcat gggattgcgc ttgccggcgg
     4201 cggagccctg ctgaaagggc tcgatcgcag gctgtcgcaa gaaacgcggt ttccggttta
     4261 tgtcgccgac gatccgctga cctgcgtcgt tcgcggaacg ggcgaggtgt tggaagaagc
     4321 ggcaatgctc gcaaaggtgc agtcacggct tgcacaacgg aggccgcccc gctgaactct
     4381 tcaggccgca ctcaaacaca tgcagatcga ctcgcgggac ggccggctcc gcgcggctcg
     4441 ctggccggct gagaactatg tcgctgtcga gaccgctcac ctggacgctc tcattgctgc
     4501 tcctgggagt cgtcgccctc ggactgtcgc gtaccggcgc caccgatccg ttgcagagcc
     4561 tcgccgtccg cgtcttctcg ccacttgact cgggcgttca ctcggttttc actccgatcg
     4621 ccgatttcgt caacaacgtc ggcagctatg gcgatctgcg caaggaaaac cgtgacctgc
     4681 gcgccgacaa cgagcgactg aacgccgccg tgaaccaact tcgtgaacaa caggcggagg
     4741 cgggacaggt cgacgctctg acccagtcaa ccaaactctt ccccgacgcc gatttcgtga
     4801 acgcgaacgt cattgcacgc gatcccagca acgtgcatga tgctgtgctc atcgacaaag
     4861 ggaaaaacga cggcgtgctt agcggcatgg tgatcatggg caaagagggc tccctgctag
     4921 gcaccgtgtc gaaagcgctc tcaacttcgg cgtgggtgcg actgacaacc gaccccgata
     4981 gcgatgtgaa cgccgaggtg caagaaacac gggcgatggc gatcgtatcg ggcgccttcg
     5041 gtcacaagtt gcaactacag ttcgtcgccg agggcacgga tgtgaaaccc ggcgatacgg
     5101 tcgtcacctc tggactcggg gggaactatc cgaagtcgct actcatcggt cgcgtcagca
     5161 aagtcgaggg tggacccacc gaactcttca aaacgatcca ggttgacccg gcggtgcgcc
     5221 ccggcacgtt ggagagcgtc attgtaatga ccagcttcgt gccgtcacgg gtagacacgg
     5281 ggcagtgatc tactacgcgg caattcccct ctccattttt atcgtcgtgc tgcaggtggc
     5341 ggccgcgcct tcgtttacct acctgggtgt tcacgccgac ctgccggtag tctggctggg
     5401 atgctgggca gccgcacgtg gccggcgtga aatgttgccg ctggtaggcg taaccggtct
     5461 ggcgttggga ttgctcgggc gcgaaccgct cggcgcctct gtactggcgc ttctgccatt
     5521 agcgggtctg gcggtactgg atgagtcgcc cgctatcgtc ggccggtttg ttaccgcgct
     5581 gctggtcgta ttcgtcggcg gcgttatgtt cgccgttatt cacgccctta catcgtttgc
     5641 gggcggtgag gggttcggat ctcctctaaa cgttctacgg atcgcccccc gggcggctgt
     5701 ccttgacgtt atgaccgccg cgctgtggta ctggccggtc cggctctttt tcggtaggca
     5761 cacccgcgcg ggtgaattcc gacgtatgtg agtgttacga tcgtccttgg tggaggggga
     5821 tgtggggatt ttggagccgt gatgggactg tttagccggg agcgtcgctg gcgcgccgaa
     5881 tctcgaacac cgcggaccga agaggatctc tctcgctcca gcaaccgtcg actgatcgtc
     5941 atgcgattgg tggttgtgct cctgttttgc gccctcgggc tccagctgct gcgtttccaa
     6001 gtttggagtg gcggcaggta tcagctcaag gccgaaagca accgactccg tctcaccgtc
     6061 actgaatcgc cccgtggtct gatctacgac cgtcaccaca acccgctggt cgctaacatt
     6121 gcgtcgtaca ccgccatcgt catcccagcc gacatcccca agggagccga ccgcgatacg
     6181 ttttaccagt tgagcggtgt gatcggcgtg ccgccggacg tcatcgagca gaaggtgacc
     6241 gacagcatca agcgcactga cccgttcacg ccggtgccca ttaaagaagg gcttgaccag
     6301 cgcaccgtcc tgacgcttgc tgagttgcgt cacacgtttc caggcgtcga cgtgaaatac
     6361 gacgcggtgc gacagtacac caactcgacg ctaatggcgc atatatacgg ctacgtcggc
     6421 aaagtggctg aggacgaata cgatcagcta aaagatcgcg gttaccagct aagcgataag
     6481 atcggcaaaa ccggcgtgga gtacgcgtac gaggatcagc tgcgcggcac gctcggcaag
     6541 caacaggtcg aagtggacgc cagcggtcga gtcctccgca cgctggacgg aatcccacca
     6601 aagcccggaa acagtctcgt cctctctatt gacctggacc tacaacggca cgtcaccgat
     6661 atcctgcagc agtcgctgat ccagtacggc tcaccctccg gcgccgccgt aatgatggac
     6721 atccataccg gcgagatcct cgcgtacgtg agcctgccga gctacgacgt aaacctcttc
     6781 tctaaaccga tatctaacaa ggactaccag gctttgctcg acgaccccgg caagccgttg
     6841 gtcgaccaca tctcagacga tcagtatccg ccaggtagta tcttcaagac cattaccggg
     6901 tcggcggccc tcgagacggg cgtggcttcg ctcaatacta cgctcacgag ccacggggct
     6961 ttttccgtaa ccaacaaccc caatgacaag gcgaacgtag tctacgactg ggcggacctc
     7021 ggtacgctcg atttcctcca cggcctgtcg atgtcatccg acatctactt ctactgcctg
     7081 gctggcggct gtcccctgct acccggtggc ggcatctcca caaactcggc cctcggcgcc
     7141 gaccggttgg ctaaattcgc acgtgacttc ggactcggat cgaagaccgg catcgacctc
     7201 cccgacgaag cgtcggggtt gattccaacg accgactgga aggcgaagac ccaaaaagac
     7261 gaaaagggaa atccgttgcc ctgggtcgtc gcggacacgt acttcatggg gatcggtcag
     7321 ggctacgata ccgcgacgcc catccagatg gttcgcctgg cagcgacgat cgccaacggc
     7381 ggcgatctgt tgcgtccgca cgtcgtccac gagatcgatg accccgcggg caacgtggtt
     7441 gtgccgccgc aacgtgaagt cgtgcgccat gtcggcgtca gcgaccagaa cctggcgatc
     7501 atccgccagg ggatgcttat ggccgtcgag ggcggcagcg cccagaacgg gttcctgccc
     7561 ggctaccaca tcgccggcaa gactggcacg gccgagtttg gcgtcgccat tcagccggcg
     7621 ggcgacagca acaacggtac atacaacgag cacggctggt acatgtcata cgcgccgtac
     7681 aacaatcctg aggttgcgct ggtggtgttc cacgaacgcg gaggtggcgc cgtctctgcg
     7741 gcaccgactg cgcagaagat ctgggattac tacttcaatc attacaaggc cggagacaag
     7801 acccccgaac tgaaagcctc gcctacggcg gctgccggga tcggccattg atccgcgatc
     7861 tgcgccgctt tgattacccg atgctgcttt cgtgcctgct gctcgtcggc tacggcctgg
     7921 cgctgatcta ttcgggttcg ctcgcgctat acggcaccag ccacgctgcg ctgacccacg
     7981 cggtgttcaa gcaggccgcc tttgccgtgg tcgggctgct gctcctcggc ctgttaacgc
     8041 agatcgatta ccgagtgtgg tccaacctgg cgcccattct ctatggattg acgatcgtcg
     8101 ggctactcgc ggtgctcgtg atcggccagc gggcctttgg atcgcgccgc tggttctcgc
     8161 ttgccgcgct tcagcttcag ccttccgaac tggcaaagtt ggt
//
LOCUS       DATCPE010000043         9208 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U7219
            k141_6678, whole genome shotgun sequence.
ACCESSION   DATCPE010000043 DATCPE010000000
VERSION     DATCPE010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955033
            Sequence Read Archive: SRR1586279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 9208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 16:49:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 640
            CDSs (total)                      :: 631
            Genes (coding)                    :: 627
            CDSs (with protein)               :: 627
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9208
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6678"
                     /isolate="SMAG_U7219"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..859)
                     /locus_tag="VIM45_01320"
     CDS             complement(<1..859)
                     /locus_tag="VIM45_01320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8819958.1"
                     /translation="MRRWCVLALTLVVILLFGALTGRGRFAAQAADLWNVQVSGDVAA
                     DGIMGTTYFPEVLNIHIGDTVVWTWATTFAPHTVTFPSGAEPPPPLIPGPDPGSLQAG
                     PGFFPIGVGPDNSGTYDGTQVANSGVPADPTIPPFQLTFTAPGIYPYICLFHPGMHGT
                     ITVLPSDASLVESPAQAFARGQADFNVLADGLRNTIGAIQSTSLAIPGGVTLHTVAAG
                     VSHPAGMTALRFLPDSITVRRGDLVSWTIADPFELHTITFTSGTNPPGLLDVRPQPSG
                     APLIVIPTQV"
     gene            1337..2125
                     /locus_tag="VIM45_01325"
     CDS             1337..2125
                     /locus_tag="VIM45_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8819959.1"
                     /translation="MNWPRCQAYYEVLNADAAFQAALYVLDDFVEPHCPLYPMNGHIA
                     ATVWPAVDAFVRQWRLPTKPAQFPDGLRGGQAYGFDDMQYRYSVRRAARLPGEFRRLL
                     VGARPDPRSVLDEYSKPYIHEDGRVDADVTPPAIRPMLPEPFCYDPTALPGARGAMKP
                     KELQRRASAVAAAVKADILAQAAAIEKRAERMGFAPRGSRYQDPGEIARGARRLYQRA
                     VLRMRYRDIADDEHLENDKSASEQAIGQTINEWAELLGVELPKV"
     gene            2207..2401
                     /locus_tag="VIM45_01330"
     CDS             2207..2401
                     /locus_tag="VIM45_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013479565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family DNA-binding protein"
                     /protein_id="HEY8819960.1"
                     /translation="MEKLALSIPEAVERSSLGRSLIYKSIADGSLPSLRAGSRRLILT
                     ADLERWLLTLRDTALTAAGR"
     gene            2409..5792
                     /locus_tag="VIM45_01335"
     CDS             2409..5792
                     /locus_tag="VIM45_01335"
                     /inference="COORDINATES: protein motif:HMM:TIGR01613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage/plasmid primase, P4 family"
                     /protein_id="HEY8819961.1"
                     /translation="MTASTTAPTAEHLAFIGHRCGLGILPPREDGSKAPLAEYEDEKG
                     RRIWEPYQRSHATPAHIAEWYANGRTGLGIVTGKISGHWNEDGENIDGLEVFEFDDRP
                     TYDAFMMAAEAVGLASLIARIRAGYEESTPGGGVHWPCRCAEISGNTKLAERPKRPNE
                     MGHPNDKRKVLIETRGEGGFIITAPSAGSVHPTGKAYVLVSGGFDTIATISPQEREAL
                     WDLARTFDQMPEKPIEPHATARSAAAAGGRPGDDFAARVDWPEILEPHGWRRVYGKLW
                     RRPGKERGWSASVDYQGSNLLYVFSSSTAFEPERGYGKFSAFALLNHGGDFTAAAKVL
                     AAHGFGEQRRNGSTPDSEATAALRAEVDVSEAGTVALTADEKHHLSDHGNSLRLVQRH
                     GNDMRYSSTAGGLFAWDGQRYQRDEDDACVTQFAAATARSIYHEANTEADPERRQALG
                     KHALKSEAAPRLAAMVALAKTHPAVRVRADAFDADRSLLNCPNGTLNTITAELHEHRR
                     EDLCTKLAGAPFDHDAGCPMWDAFLFRIMGGNEALIRFLQRAIGYSLTGDASEQVLFL
                     AHGKGANGKSTLLETVRGVLGDYARATPFSTFLSRGDGDRATNDLAALSGARFVSATE
                     ADAGKQLSESVTKQVTGGDLVKARYLFREYFEYLPQFKVWLAANVKPTIQGTDEAIWR
                     RVRLIPFDVTIPEAERDRHLAEKLRSELSGILAWAVRGCLQWRKDGLGVPEEVRRATD
                     EYRTEQDTIAGFLDDACVLHPQAQVPSGRLYHEYAGWCANSGEKPVTQKALASLLTER
                     GFVSVRLGKERTRTWQGMGLRSDTDPTDGGRMDQADALGRVKGDPSQDFSHEGEYRKN
                     ASICVRQGKASAKAAALSTETHGSGCVGNPASRTDCWDMLGSEPRARRATRSAVARTA
                     GYSERTARVKATVLLTIANIAAAIAVETEKRNAAIKADGIARPSLARVGNPSRSGTVN
                     TSANVGGSVRPRGHAATARPNARFLNFHACGIFIHGASSVTTLGSACNFSARRCHDVV
                     RSFSYRAPCSSRSTACARSRPLGWVCRSFCTWSQADSAMLRLGRLVHLNQRRLHWMEG
                     WVQRHGIRAIVPGRLIPGLRHDALLAGPVSAPIPPSAWG"
     gene            complement(5887..6426)
                     /locus_tag="VIM45_01340"
     CDS             complement(5887..6426)
                     /locus_tag="VIM45_01340"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEY8819962.1"
                     /translation="MPGFSRLRAPERAYSASAQARSAALANCDVDASRLPLNDEEQAV
                     LDQTNAYRAAYSLQPLQLSYSLTVNAEWKASDMAANHYGAHDDGFRTWDQRFRDCGYN
                     MPDAYMGENLAGGNAGSVATLEQWQTSPHHNENLLNPNYSVVGIKRAKAPDASDIHVW
                     YWAMELASGAQPDVDLSPR"
     gene            6661..6807
                     /locus_tag="VIM45_01345"
     CDS             6661..6807
                     /locus_tag="VIM45_01345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8819963.1"
                     /translation="MGLVLGHHCCERTRQLDLAKADALDDMGERHAAHELILQSLRPP
                     PGFA"
     gene            complement(6958..8142)
                     /locus_tag="VIM45_01350"
     CDS             complement(6958..8142)
                     /locus_tag="VIM45_01350"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEY8819964.1"
                     /translation="MDRERILQLAHATDATVLAARRHIHQYPELSFEERETASFVADR
                     LRSLGYAPQTNIAGYGVKVVLNGGRPGRTVALRADMDALPLHEPTGLPFASSRPGAMH
                     ACGHDAHTAMLLGAAQAILEIRDELVGNVVFLFQPAEEKPPGGALPMIEAGVLEDPPV
                     DCVFGLHQSPTYDVGQFGVTGGPRSASSDTFRVSIISKGGHAARPHATADPIAAAGMA
                     IAGIHQIASRRLSAMQPVVITIGTIHGGTKENIIPELVTMSGTVRCFDEAIRREVPEM
                     IRNVAETAAAMFGAAATLDYEYGYPVTKNDDAMAAVARRAAERVFGSANVLTPEPAMP
                     SEDFSYFLQRVPGAFASIGAGTPGATNRGSTHSPSFILDESALPLGVAFYLSLVSDLL
                     GA"
     gene            complement(8442..>9208)
                     /locus_tag="VIM45_01355"
     CDS             complement(8442..>9208)
                     /locus_tag="VIM45_01355"
                     /inference="COORDINATES: protein motif:HMM:TIGR01409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HEY8819965.1"
                     /translation="WRMPMTTRRGFLQFGGATALALVAAPRLLHLKAPSVHAQAIPGA
                     PTFRGAAGTAVVGPVSLAAGITVLRAQHNGTGNFIVNTFVPNDGYSPADAFTQSQTTD
                     NALVYDVIGSYKGGSVVVTALNAPYYLAVNASAAWQVSVEQPLPETVAATQGTSFSSK
                     GQDITPYFTLPDGIAQIALQADKSSAITGFLYHLDDLGGEAIQAGEMGHTGAIFFFND
                     PANQVSYPINLPDDGPYVFFVTNDLHDTTAWTVSFA"
BASE COUNT         1720 a         2870 c         3019 g         1599 t
ORIGIN      
        1 acacctgcgt cggaatgacg atcagcggcg cgcccgatgg ttggggtcgc acgtcgagga
       61 ggcccggcgg gttcgtgccc gaggtgaatg tgatcgtgtg aagctcgaac gggtcggcga
      121 tcgtccacga aaccagatcg ccgcggcgca ccgtgatgct gtccggcagg aagcgtagcg
      181 cggtcatccc agccgggtgg ctaaccccgg cggcaacggt gtgaagcgtg acgccacccg
      241 ggatggccaa actggtcgat tggatggccc caatcgtgtt ccgtagacca tcggccagca
      301 cgttaaagtc tgcctgcccg cgtgcgaagg cttgcgccgg gctctcgacc aggctggcat
      361 ccgaaggcag caccgtgatg gtgccgtgca tgccgggatg gaagagacaa atgtacggat
      421 agatgcccgg tgctgtgaag gtcaactgga acggcggtat tgtgggatcc gccggtacgc
      481 cggagttagc gacctgggtc ccgtcgtacg tgccgctgtt gtcgggtccc acgccgatgg
      541 ggaaaaaacc cggccccgcc tgcagcgagc cgggatcggg accggggatc agaggtgggg
      601 gcggctcagc gccgctcggg aacgtgaccg tgtgcggcgc aaaggtcgtg gcccacgtcc
      661 aaaccacggt gtctccaatg tgaatattca atacctcagg gaagtaggtg gtgcccatga
      721 taccgtcggc tgcgacgtca ccgctgacct gcacattcca gaggtccgcg gcctgagcgg
      781 cgaagcgccc ccggccggtc agcgccccaa agagcaagat caccaccagg gtaagcgcaa
      841 gcacgcacca tcgtcgcatc gcggaatctc cttttcgctg aatgtgtttc ccaatgaccc
      901 aggccttcgc acaatccacg cgattgtacc acctcgttcg agaccgtcaa gcgaagtttg
      961 tttgccgagc cgcgtcggaa cttgcttcga tcgtgttggt gatgaatcgg cgattgaagc
     1021 ctgtggctac tgtgcacgcc taaattctgg tgagccgggt gggattcgaa cccacgatca
     1081 atggattaaa agtttcgtgg aacccgtccg cacacggcca ctttcacgcc taattccccc
     1141 ggtactgtcc gccacttttc gccgttgtcc gcccgcattg gtgtcagaag tggtgtcaag
     1201 actgttacgg aacggtgacg cgatagaata gaaccgcccg cgctgcacca acagccgggc
     1261 ggatggcaaa cgactggata ggagccgttc acatggacag tatacccgcc ggcgcccgct
     1321 ccaaacgccg gaggaattga actggccgcg ttgtcaggct tactacgaag tgctcaacgc
     1381 cgacgccgcc tttcaagccg cgctgtacgt gctcgatgac ttcgtagaac ctcactgccc
     1441 gctgtatccg atgaacgggc acatagcagc gaccgtctgg ccggccgtag atgcatttgt
     1501 caggcagtgg cggctgccga cgaagccggc acagttccct gacggcctga ggggcgggca
     1561 ggcttacggc ttcgatgaca tgcagtaccg ctattctgtc cggcgcgcgg ctcggcttcc
     1621 cggcgaattc cgccggctgc tagtcggagc acggccggac ccgcgaagcg tgctcgatga
     1681 atacagcaag ccctacatcc acgaagacgg ccgcgttgat gccgatgtca cccccccggc
     1741 cattcggccg atgctgccgg aacccttctg ctacgacccc accgcgctgc ccggtgcccg
     1801 tggcgcgatg aagccgaagg agctgcaacg tcgggccagt gccgtcgctg ccgccgtcaa
     1861 ggccgacatt ctggctcagg cggctgcaat cgagaaaaga gctgagcgta tgggattcgc
     1921 accccgcggg tcacgctacc aagatcctgg cgagattgcc cgcggcgctc gccgcttgta
     1981 tcagcgtgcc gttctgagga tgagatatcg agatattgcc gacgacgagc atcttgagaa
     2041 cgataaaagc gcgtcggagc aagccatcgg tcagaccata aatgaatggg ccgagctgct
     2101 tggggtcgag ctaccaaagg tttgagtttc cacactgaaa acttgtggac cgcctaccgc
     2161 gtccacgtga agctgtccgt agcagaaacg ctacggaggc ccagtcttgg aaaaactcgc
     2221 gttatccatt cccgaggccg tggaacggtc gtctctggga cgctcgctga tctacaagtc
     2281 gatcgcggat gggtctttgc catcgctccg cgccggatcc cggcgtttga ttcttacggc
     2341 ggacttggaa cgctggctgc tgaccctgcg tgatactgcg cttaccgcgg ccgggcggta
     2401 acgccgcgat gactgcgtcg accactgccc ccaccgctga acacctggcc ttcatcgggc
     2461 atcgctgcgg gctgggcatc cttccgccac gtgaggatgg gagcaaggca ccgcttgctg
     2521 aatacgaaga tgaaaagggc cgacgcatat gggagcctta ccagcgctcc cacgcaaccc
     2581 ctgcgcatat cgcggagtgg tatgcaaacg gacgtacggg gctgggcatc gtaaccggga
     2641 agatcagcgg ccattggaac gaagacggcg agaacatcga cggcttggag gtgttcgagt
     2701 ttgatgatcg cccgacctat gacgctttca tgatggctgc cgaggccgtc ggtctggcat
     2761 cgctgatcgc ccgcattcgc gccggctacg aggagtcgac ccctggcggt ggcgtccact
     2821 ggccctgccg atgtgcggag atcagcggca acacgaagct tgccgaaagg ccgaagcgcc
     2881 ccaacgagat gggccaccca aacgataagc gcaaggtgct gatcgaaacc cgcggtgagg
     2941 gcggtttcat tatcacggcc ccatccgccg gtagcgtcca cccgaccggc aaggcctacg
     3001 tgctggtcag cggcggcttc gacacgatcg cgaccatttc gccacaggag cgagaagcgc
     3061 tctgggatct ggcgcgcacg ttcgaccaga tgcccgagaa gccgatcgag ccgcacgcca
     3121 cggcccgcag tgcagcagcg gccggcggca gacccggtga cgacttcgcg gcgcgtgtgg
     3181 attggccgga gattcttgaa ccgcacggat ggcgacgcgt ttacggcaag ctctggcgcc
     3241 gccccggaaa agaaaggggc tggtcagcga gcgtcgatta ccagggtagc aacctgctgt
     3301 acgtctttag cagctcgaca gccttcgagc ccgaacgcgg ctacggcaag ttctcggcct
     3361 ttgcgctgtt gaaccacggc ggcgacttca cggctgcggc gaaggtgctg gcggcgcatg
     3421 gcttcggtga acagcggcgc aacggcagca cacctgacag cgaagcaacg gcggcgctgc
     3481 gcgcggaagt ggacgtcagc gaagcaggaa ccgtcgcgct gaccgccgac gaaaagcacc
     3541 acctttccga tcacggtaat tccctgcggc tggtgcagcg gcacggcaac gacatgcgct
     3601 atagctcaac ggccggtggg ctcttcgcct gggacggcca acgctatcag cgtgacgaag
     3661 acgacgcctg cgttacccag ttcgcggccg ccacggcgcg cagcatctac cacgaggcga
     3721 acaccgaagc agaccccgaa cggcgtcagg cgctcgggaa acacgcgctg aaaagcgagg
     3781 ccgccccacg tctggccgcc atggtggcac tggcgaagac acacccggcc gtgcgtgtac
     3841 gggccgacgc cttcgacgct gaccgttcgc tgctcaactg ccccaacggc acgttgaaca
     3901 ccatcaccgc cgagctgcac gaacaccggc gcgaggacct gtgcacgaag cttgctggcg
     3961 ccccgtttga ccacgacgca ggttgcccga tgtgggatgc gttcctcttc cgcatcatgg
     4021 gcggcaacga ggcgttgatc aggttcttac aacgtgccat cggatactcg ctcaccgggg
     4081 atgcgagtga gcaggttcta tttctggcgc atggcaaagg ggcgaacggt aaatcgacgc
     4141 ttttggagac ggtgcgcgga gtgctcgggg attatgcccg agcaaccccg ttcagcacgt
     4201 ttcttagccg cggggacggg gaccgggcaa caaacgacct ggccgcgctg agcggcgccc
     4261 ggttcgtctc tgctaccgaa gccgacgccg gtaagcagct ctcggaatcg gtgacgaagc
     4321 aagtaaccgg cggcgatcta gtgaaagccc gctacttgtt ccgcgaatac ttcgagtacc
     4381 taccgcagtt caaggtgtgg ctcgcagcca acgtcaagcc cacgattcag gggacggacg
     4441 aagcaatctg gcgccgcgtc cgcctgattc ccttcgacgt cacgatcccc gaagcggaac
     4501 gggatcggca cctggccgag aagttgcgca gcgaactgtc cggcatcctg gcatgggccg
     4561 tgcgcggctg cctgcagtgg cggaaggacg ggctcggcgt accggaggaa gtacggcgag
     4621 cgacggacga atatcgcact gagcaggaca cgatcgccgg ttttttagac gatgcatgcg
     4681 tcctccaccc gcaggcccaa gtcccgagcg gccggctcta ccacgagtac gcaggctggt
     4741 gcgcgaatag cggcgaaaag ccggtaacac agaaggcgct tgcgagcctg ttgacagaac
     4801 gcggttttgt atctgtccgt ttgggcaaag agcgcacgcg gacatggcag ggcatgggac
     4861 tgcggagcga taccgacccg actgatggcg gacgcatgga ccaggcggac gcattaggac
     4921 gcgtaaaagg ggatccttcc caagatttct ctcatgaagg agagtaccgg aaaaacgcgt
     4981 ccatatgcgt ccgccaaggc aaggcgtccg ccaaagctgc ggcgctctcc actgaaacgc
     5041 acgggagcgg gtgcgtggga aaccccgcat cacggaccga ttgttgggat atgttggggt
     5101 ccgagcctcg cgcgcggcgg gctacacgaa gcgcggtggc gcggacggcc ggctactcgg
     5161 agcggacggc acgggttaag gcgacggtac tgttaacaat tgctaacatt gccgccgcca
     5221 tcgctgtcga gacggaaaag cgcaacgccg cgatcaaggc ggatggcatc gctcgtccga
     5281 gcctcgcgcg cgtgggaaat ccctcccggt cggggacggt caacacttcc gccaacgttg
     5341 gcggaagtgt caggccacga ggccatgccg ccaccgccag accaaacgct cgattcctga
     5401 attttcatgc ttgcggaatt ttcatacacg gcgcaagctc cgtgaccacg ctcggttcgg
     5461 cgtgcaactt ctctgcacgg cgttgccatg acgtggtgag gtcgttctcc tacagagcgc
     5521 cctgctccag ccgctctacg gcgtgtgcga gatcccggcc gctcgggtgg gtatgtcgat
     5581 cattctgtac atggtcacaa gccgattccg cgatgctgcg cctcggtcgc ctggtgcacc
     5641 tgaaccagcg acgcttgcac tggatggagg ggtgggtgca gcgccatggg attcgagcga
     5701 tcgtgccagg acggctgata cccgggctgc gacacgatgc gctgttggcc gggcctgttt
     5761 ccgcacccat accaccgtct gcttggggct gacggtctga cgcggagctg cttgcacgcc
     5821 gctggtcggc cggcgcgccc gcgtcagagc tccggccgga ggtcgaggat cagtgcggct
     5881 gcgacgtcaa cgcggtgaca gatcgacatc cggctgcgcg ccgctggcca gctccatcgc
     5941 ccagtaccac acgtggatgt cgctcgcgtc gggcgctttt gcccgcttga tgccgacgac
     6001 gctgtagttc gggttcagca ggttctcgtt gtggtgcggc gaggtctgcc attgctcaag
     6061 ggtggcgacg ctgccggcgt tgcccccggc caggttttca cccatgtacg cgtcgggcat
     6121 gttgtagccg cagtcgcgga agcgctggtc ccaggtgcgg aagccgtcgt cgtgggcgcc
     6181 gtagtgattg gccgccatgt cgctcgcctt ccactccgcg ttgacggtca gcgaatagga
     6241 gagctgcagg ggctgcaggc tgtaggcggc gcggtaggcg ttggtctggt cgagcaccgc
     6301 ctgctcttcg tcgttcagcg gcagccggct ggcatcgacg tcgcagttgg cgagcgccgc
     6361 cgaacgcgcc tgggcggatg ccgaataggc gcgttccggc gcgcgcagcc ggctgaagcc
     6421 aggcactacg gctacgagta gcgcagtggc cagagggagc catatacgcc tgggccggca
     6481 cgcggttgag ggctgatgaa acacgaacat tccatggcct cgttacttca tgcgtcggcc
     6541 gagcgcccat catgcgtgag ccttcttcag taacgtgacg gtaaggactg tcttcttaga
     6601 caaacataat tcggcagccg cgccgcggca gtcaccccgg gcagcgggaa gaggagtgcg
     6661 atgggcctgg tactcggcca ccattgctgt gagcgtacgc ggcagctcga cctcgcaaag
     6721 gcggacgcgc tggacgatat gggtgagcgg cacgcggcac acgaactgat cctgcagtcc
     6781 ctgcggccgc cgcccgggtt tgcttgatat gggcggcact ctccgggggc ataaccttcg
     6841 gataggtcgg ccccagcaac acgactgtaa gcgcgttccc ttgcgcctcc aacagtacgc
     6901 acacgatgtt gccatcgtgt gcgggagtat gaccgcgccg ggcgtcacgc cgcagcgcta
     6961 cgcacccaag agatccgaga cgagcgagag gtagaacgct acgcccaacg gcaacgcact
     7021 ctcgtcgagg atgaagctcg ggctgtgggt agacccgcgg ttcgtcgcac ccggcgttcc
     7081 ggcgccgatg ctagcgaaag cgcccggcac gcgctggagg aagtaggaga agtcctccga
     7141 cggcattgcc ggctcgggcg tgagcacgtt ggcgcttcca aagacccgct ctgcagcgcg
     7201 acgagcgacc gcggccatgg catcatcgtt cttcgtgacc gggtagccgt actcgtaatc
     7261 cagcgtcgct gcggcgccga acatcgccgc agcggtctcg gcaacgttac ggatcatctc
     7321 cggcacctcc cggcggatcg cctcgtcaaa acaccgcacc gtgccgctca tggtgaccag
     7381 ctcagggatg atgttctcct tcgtacctcc gtggatggtc ccgatcgtaa tcaccaccgg
     7441 ttgcatcgcc gacagccggc ggctcgcgat ttgatggatg cccgcgatcg ccatcccggc
     7501 agcagcgatg gggtccgcgg tggcgtgcgg acgggctgca tggccgcctt tgctgatgat
     7561 cgaaacccgg aaggtgtccg agctcgcgga acgcggaccg cccgtcacgc cgaattgccc
     7621 gacatcgtag gtcggactct ggtgcagacc gaagacacaa tcgaccggag ggtcctctaa
     7681 cacacccgcc tcaatcatcg gcagcgcgcc gccgggtggc ttctcctccg ccggctggaa
     7741 gagaaagact acgttgccga caagctcatc gcgaatttcc aggatcgcct gggcggcgcc
     7801 gagcagcatc gctgtgtggg cgtcgtggcc gcaggcgtgc atcgcaccgg gacggctgct
     7861 ggcgaagggc aaccctgtgg gctcatgcag tggcaatgca tccatatcgg cgcgcagcgc
     7921 gacagttcgc cccggacggc cgccgttcag caccactttc accccatagc cggcgatgtt
     7981 cgtctgcggc gcatagccga gggagcgcag tcggtccgcg acgaaggatg cggtttctct
     8041 ctcctcgaaa ctcaactcag ggtactggtg aatatggcgc cgagccgcca acaccgtggc
     8101 gtccgtggcg tgggcgagct gtaggattcg ttccctgtcc actctccggc tcctctactc
     8161 caggacggtg tgagagtgag tgtaatggac gagactccgg aagcggctgg tgttgcgttg
     8221 atctgccgaa gagacacgag catgcaatcg tgtatgccaa cggcgcagga gtgtgacgct
     8281 tgtcgtcgtg actcccgcgt gtggacaaac cggctcgcga cggagaccga cgaccgatac
     8341 tcaggcaact gcctcgctgc ggcaggaagc accgcggcca gcgcttcctg tgttcccgcc
     8401 ggccaaacca gtcttcgggc agtccaaccg ccacggtctc gtcaggcgaa gctcaccgtc
     8461 caagcggttg tgtcgtgaag atcgttggtg acgaagaaca cgtagggacc gtcatcgggc
     8521 agattgatgg gataactcac ctggttggcg gggtcgttga aaaagaagat tgccccggtg
     8581 tggcccatct cgcccgcctg gatcgcttcg ccacccagat cgtcgagatg gtagagaaag
     8641 ccggtgatgg ccgagctttt gtccgcctgc aacgcgatct gcgcgatgcc gtcgggcagc
     8701 gtgaagtagg gcgtgatgtc ttgccctttg ctggagaagc tcgtgccctg cgtggcggca
     8761 accgtctcgg gtagcggctg ctcgacgctc acctgccagg cggcgctcgc gttcacggcc
     8821 aggtagtagg gggcattgag ggcggtgacc accaccgagc cgcctttgta gctgccgatg
     8881 acgtcgtaca cgagcgcatt gtccgtggtc tgcgactgcg tgaaggcatc cgccgggctg
     8941 tagccgtcat tcggtacgaa agtgttgacg atgaaattgc ccgtgccgtt gtgttgggca
     9001 cgcagcaccg tgatgccagc ggccaggctg accggtccca cgaccgccgt gccggccgcg
     9061 ccccggaacg tgggtgcgcc ggggatcgcc tgcgcgtgga cggatggcgc cttcaaatgc
     9121 agcaggcgtg gcgcggccac gagcgcgagc gccgtggcgc ccccgaactg caagaaaccg
     9181 cgtcgtgtgg tcatcggcat tcgccacc
//
LOCUS       DATCPE010000044         5638 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U7219
            k141_39484, whole genome shotgun sequence.
ACCESSION   DATCPE010000044 DATCPE010000000
VERSION     DATCPE010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955033
            Sequence Read Archive: SRR1586279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 5638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 16:49:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 640
            CDSs (total)                      :: 631
            Genes (coding)                    :: 627
            CDSs (with protein)               :: 627
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5638
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39484"
                     /isolate="SMAG_U7219"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1023
                     /locus_tag="VIM45_01360"
     CDS             <1..1023
                     /locus_tag="VIM45_01360"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY8819966.1"
                     /translation="DANPNQTVLADLVARLEGAETGVAAASGMGAIAAAIMSGLKQGD
                     RLVAGHDLYGRTMGMLRTSLAALGVSTDFVDLTDTDAAAAALAQPATAVIVESVSNPL
                     VRIPDLPALAELAHRAGARLIVDNTFASPYHCRPLAFGADVVVHSGTKYLGGHSDVTN
                     GVLVGSVDFVQQARSFIVTFGASASPFDCWLTARGIKTLAVRMQKASANALQICCFLA
                     GCPEVEAVHYPGLSSHPHHQRARSLLCDGFGAMAAFELRGGEAAASRFVKGLKHIRLA
                     PSLADVSTTISHPAKTSHRGYTAEQREAIGVFPGLIRLSVGIEHADDIIEDLRRGLDA
                     AGRGAS"
     gene            1108..1542
                     /locus_tag="VIM45_01365"
     CDS             1108..1542
                     /locus_tag="VIM45_01365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEY8819967.1"
                     /translation="MRVSTKGDYGVRALIELALHYGEGPLQSAEIAARQRIPEPYLDH
                     LLTTLRRAGFIRSIRGPQGGHELVREPGDIHMDNALEALEGSLSPSSCLDESSACAKH
                     GGCPQRDVWQEVYDVTRGILHRTTIAELAAKERRPAGGRYYI"
     gene            1573..2508
                     /gene="cysK"
                     /locus_tag="VIM45_01370"
     CDS             1573..2508
                     /gene="cysK"
                     /locus_tag="VIM45_01370"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018249271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEY8819968.1"
                     /translation="MQDVATRTVSIVDSVLDLVGNTPLVRLNRLPGPRSAEVLGKLES
                     INPAGSVKDRIAVSMVEDAERQGLLAPGTTIVEPTTGNTGIGLAMVAAVKGYRLILTM
                     PEDMSLERRRLLARYGAAFVLTPAIEGMTGAVYAAEQLLAKHPDYFMPQQFLNAANPE
                     IHRQTTGPEILRATDSRIDAFVAGVGTGGTITGVGEVLRERIPDVLIVAVEPVRSQVL
                     RGHRAGMHAIQGIGASFVPGVLNRDVYQELIAVGDDDAIEMSRRLAREEGLLVGISSG
                     ANVVASLKVAKRLGPGKRVVTMLPDTGERYLSMGL"
     gene            2602..3711
                     /gene="moeB"
                     /locus_tag="VIM45_01375"
     CDS             2602..3711
                     /gene="moeB"
                     /locus_tag="VIM45_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HEY8819969.1"
                     /translation="MAVSVYIPTPFRRLTGNRTRVEMGGETVAEVLAELDNAYPGFSE
                     LLWDEKREIPAHINVYLNNREINSLQGQQTPVADGDEVAVIPAIAGGSGTALRADQVT
                     RYSRHIIMPQVGPVGQRKLMASKVLVVGAGGLGSPVALYLALAGVGTLGIVDFDIVDL
                     TNLQRQILHQNDDVGRPKVESARETLNAYNPDVNVVTHEVPLTSENAMQIIPQYDVVV
                     NGADNFATRYLVNDACYLAKKPLVDGSILLFDGQASTYLPGKGCYRCLFPEPPPPGMV
                     PSCAEAGVLGALCGTVGSIQATEVLKLILEIGDPLVGRLLLYDALSMEMRVVKTRRDP
                     KCPLCGDNPTVTELIDYDAFCGGPPHKEGAPVAAG"
     gene            3771..4049
                     /locus_tag="VIM45_01380"
     CDS             3771..4049
                     /locus_tag="VIM45_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014375008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD family protein"
                     /protein_id="HEY8819970.1"
                     /translation="MAVEVRLTTVLQKVTGGRKTVSAEAGNVDELLRNLDSDYPGFLH
                     QVQGDDGQLHRFVNIYVNDEDIRYLEKLDTPLKNGDVVSILPALAGGT"
     gene            4116..5054
                     /locus_tag="VIM45_01385"
     CDS             4116..5054
                     /locus_tag="VIM45_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HEY8819971.1"
                     /translation="MLYSNILDAIGNTPLVGLPQTSPNPNVKIYAKLEGQNPSGSVKD
                     RIAKYMIERGEQDGSLTHDKTVLEPTSGNTGIALAMIGRVKGYQVMVVIPESVSEERS
                     QLLRAYGAEVVYSEGLKGTNGSIAVAKQMVAEAPQRFFMPYQYGNDANPLAHYETTGP
                     EIVRDLPDVDVFIAGLGTGGTLTGAGRYLKEYNPNIKVIATVPHPGDLVQGLRSLEEG
                     FIPPVLDESVLDGRVVVDSRTAFAATKELMQKEGIFAGISCGAVIKTAQRVASRMESG
                     EIVVILADGGWKYLSTALWTMDFSDLPEDIDSKVWW"
     gene            5060..5458
                     /locus_tag="VIM45_01390"
     CDS             5060..5458
                     /locus_tag="VIM45_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017987367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HEY8819972.1"
                     /translation="MPLTLPRSYVDEIVAHAQEENPNECCGVIAGDNGVAMRLFKAVN
                     AEHSPYRYNIDSRELLKIYREIDDNGWEMMVIYHSHTHTPAYPSPTDLNLAGYPDAYY
                     LLVSLTWDPPDIRAYRIIDGAVSQEALFVE"
     gene            5448..>5638
                     /locus_tag="VIM45_01395"
     CDS             5448..>5638
                     /locus_tag="VIM45_01395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8819973.1"
                     /translation="MSSKLNGLTIQGSNAGGAMVNEVHGRLNIPVQPIPKQTAEERVK
                     NWDEVYRGFDLESAIIEANR"
BASE COUNT         1134 a         1724 c         1759 g         1021 t
ORIGIN      
        1 gacgccaatc caaaccagac cgtgcttgca gacctcgtcg cacggctgga aggggcggag
       61 accggtgtgg cggcggccag cggcatgggt gcaatcgcag cggcaattat gtcggggctg
      121 aagcaaggcg accggttggt cgcgggccac gacctttatg gccgcacgat gggaatgctg
      181 cgcacgtccc tggcggcgct tggggtcagc accgacttcg tcgatctgac cgacaccgat
      241 gccgcggccg cggcgctcgc gcagccggcg actgcagtaa tcgtggaatc ggtcagcaat
      301 ccgctggtgc gcatccccga cctgcctgca ctcgcggagc tggcccaccg cgccggcgca
      361 cgcctgatcg tggataacac cttcgcctct ccgtatcact gccgtccgct cgcttttgga
      421 gccgacgtcg tggtacatag cggcaccaag tatctgggcg ggcatagcga cgtcaccaac
      481 ggcgttctgg ttggctcggt tgatttcgta cagcaggcga ggagcttcat tgtgacgttt
      541 ggcgcttcgg ccagcccatt tgactgctgg ctaacggcgc gcgggatcaa aacgctcgcc
      601 gtgcgtatgc agaaggcgtc ggccaacgcg cttcaaattt gctgcttcct cgccggctgc
      661 ccagaagtcg aggcggtcca ttatccgggg ctgtcgtcgc acccgcatca ccagcgcgcc
      721 cgtagcctgc tgtgtgacgg attcggcgct atggcggcat ttgagctgcg tgggggcgaa
      781 gccgccgcat ctcgcttcgt gaaggggctc aagcacatcc gcctggcgcc aagcctcgcc
      841 gacgtcagta cgacgatctc ccatccggcc aagacgtcgc accgtggcta cacggccgag
      901 cagcgtgagg cgatcggtgt cttccccggc ctcatccgtc tctccgtcgg catcgagcac
      961 gccgacgaca ttatcgagga tctgcgacga gggctcgacg ctgccggacg gggcgcctcg
     1021 taacgccgcg gcacattctc ttgcaagctt tactttcttc atctacaatg ttgacagatt
     1081 tagtcaggta tagcagcggg gttttcggtg cgggtttcca ccaagggcga ctacggagtt
     1141 cgcgccctca ttgagctagc gctgcattac ggcgaagggc cgctgcagag cgccgagatc
     1201 gcggcgcgcc agcggatacc ggagccgtac ctggatcatt tgctcacgac gcttcgccgt
     1261 gccggcttca ttcgcagcat tcgcggcccg cagggcggac acgagttggt acgcgagccc
     1321 ggcgacattc acatggataa cgctctcgaa gcgctcgagg gatcgctctc gccgagctcg
     1381 tgtctcgacg agagcagcgc gtgcgccaag cacggcggct gtccacagcg cgatgtctgg
     1441 caagaggttt acgacgtaac tcgagggatc cttcaccgaa ccacgatcgc cgagctcgcg
     1501 gcaaaagagc gacgcccggc cggcggccgg tactacattt aagcccgatt aggcaggaag
     1561 tttcgaacgc acatgcagga tgtcgccacc cgtaccgttt ccatagtcga cagcgtcctt
     1621 gacctggtcg gcaacacgcc gcttgtgcga ctcaaccggc tgccaggccc tcgcagcgcc
     1681 gaggtgctgg gcaagctcga atcgatcaac ccggccggca gcgtcaaaga ccgcatcgcc
     1741 gtctcaatgg tggaagacgc cgagcggcag ggtctactgg ctcccggcac aacgatcgtc
     1801 gagccgacga ccggcaacac cggcatcggc ctcgccatgg ttgccgcggt gaaaggctac
     1861 cgactcatct tgacgatgcc cgaggatatg agccttgagc gccgtcgatt gctggcccgt
     1921 tacggcgccg cgttcgttct caccccggcg attgagggaa tgaccggagc ggtatacgcg
     1981 gccgaacagt tgctcgcaaa acatccggac tatttcatgc cgcaacagtt tctgaacgcc
     2041 gccaacccgg aaattcaccg acagaccact ggaccggaga tccttcgggc gaccgacagc
     2101 cggatcgacg cttttgtggc cggagtcgga accggcggca ccatcacggg tgtgggcgaa
     2161 gtgctccgcg agcggatccc ggacgttctc atcgttgccg tcgagccggt gcgttcgcag
     2221 gttttacgcg ggcatcgggc tgggatgcac gcgattcagg gcattggcgc aagctttgtt
     2281 cccggtgtcc tcaatcgcga tgtgtaccag gaattgatcg ccgtcggcga tgacgatgcg
     2341 atcgagatgt cgcgccgcct cgcgagggaa gaggggctgc tggttggcat ttcatcggga
     2401 gcaaacgtcg tcgcttcgct aaaggtggcg aaacggctcg gtccgggcaa gcgcgtggtg
     2461 acaatgcttc cggacacggg cgagcgatac ctgagcatgg ggctatgacg ggcaagctat
     2521 tcgccgaaac tggcgcagtg tagtgtactg aagaacgcgg gcgctcgcgc accggttcca
     2581 gcacagagga gacagtccga aatggcagtc agcgtatata ttccaacgcc gttccggcga
     2641 cttaccggca atcgcacccg cgttgaaatg ggcggcgaaa ccgtagccga ggtactggcg
     2701 gagcttgata acgcctatcc gggcttcagc gagcttttgt gggatgagaa gcgcgagatc
     2761 ccggcacaca tcaacgtgta tttgaacaac cgcgagatca acagcctgca ggggcagcaa
     2821 actccggttg ccgacggcga tgaagtcgcg gttattcccg caattgcggg tggtagcggc
     2881 accgcgcttc gcgccgacca ggtgacccgc tattcgcgac acatcatcat gccgcaggtt
     2941 gggccggtgg ggcagcgcaa gctgatggcg tctaaggtgt tggtggtcgg agccggcggt
     3001 ctgggctcgc ccgtcgccct ttaccttgca ctcgcgggtg tcggaacgct ggggattgtc
     3061 gacttcgata ttgtagacct gaccaacctc cagcggcaga tccttcacca gaacgacgac
     3121 gttgggcggc caaaagtcga atcggcgcgc gagacgctca atgcgtataa ccctgacgtc
     3181 aacgtagtaa cgcacgaagt gccgctaacg tcggaaaacg cgatgcagat tatcccccaa
     3241 tatgacgtcg tcgtgaacgg agcggataac ttcgccaccc ggtacctggt gaacgacgcc
     3301 tgctatctgg cgaaaaagcc gctagtagat gggagcatcc tcctattcga cggccaggca
     3361 tcgacctatc tgcccggcaa gggctgctac cgttgcctct tccccgaacc gcctccaccg
     3421 ggcatggtcc cttcctgcgc cgaagcgggg gtgctgggcg ccctttgcgg taccgtcggc
     3481 tccatccagg ccacggaggt gctgaaactg attctcgaaa tcggcgaccc gctcgtcggc
     3541 cggctcctgc tctacgatgc gctttccatg gaaatgcggg tggtgaagac gcgccgcgat
     3601 ccgaagtgtc ctctttgcgg ggacaacccg actgtcaccg aactcatcga ctatgacgcc
     3661 ttctgcggcg gtcctccgca caaggaaggc gctcccgtgg cggcgggata atctcacaat
     3721 attcgcggcg ccgccccgcc gggaggtcgc caaccccggg agaacagtag atggccgtag
     3781 aagtccgact taccaccgtg ctgcagaagg tcaccggtgg cagaaaaacc gttagcgccg
     3841 aggccggcaa tgtggacgag ttgctcagaa acctcgacag cgactatccg ggcttcctgc
     3901 accaggtcca gggagacgac ggccagcttc atcgcttcgt caacatctat gtgaacgacg
     3961 aggacatccg ctaccttgaa aagctcgata cgccgctgaa gaacggcgac gtcgtctcaa
     4021 tcctgccggc gcttgccggc gggacgtaga acgggcggtc tcctcactcg caaagttccc
     4081 gtggcgcgaa cgccgcccgc tgctcggagg ggagcgtgtt gtactcaaac attctcgacg
     4141 cgattggcaa tacgccgttg gtgggcctgc cgcaaacgag ccccaatccg aacgtcaaaa
     4201 tttatgccaa gctcgaagga cagaatccct cgggcagcgt gaaagatcgc attgccaagt
     4261 acatgatcga gcgtggggag caggacggca gcctgaccca cgacaagacc gtgctcgaac
     4321 cgacgagcgg caacacgggc atcgcactgg cgatgatcgg tcgcgtcaag ggctaccagg
     4381 taatggtggt cattcccgag agcgtcagcg aagagcgcag ccagttgctg cgggcatacg
     4441 gcgccgaggt cgtctattcc gaagggctga agggaacgaa cggttccatc gccgtggcta
     4501 agcagatggt cgccgaagcg ccgcagcgct tcttcatgcc ctaccagtac ggcaacgacg
     4561 ccaatccgct ggcgcactac gaaaccaccg gtccggaaat cgtacgtgac ctgcccgacg
     4621 tggacgtgtt tatcgccgga cttggcaccg gagggacgct gaccggcgcc ggtcgctacc
     4681 tcaaggaata caacccgaac atcaaggtca tcgcgaccgt cccgcatccg ggcgacctcg
     4741 tgcagggtct gcggagcctc gaagaaggct tcattcctcc cgtactcgac gagtcagttc
     4801 tcgacgggcg tgtcgtggtc gatagccgca ccgcgttcgc cgcgaccaag gagttgatgc
     4861 agaaggaagg catatttgcc ggcatatcct gtggggcggt gatcaagacg gcgcagcgcg
     4921 tcgcatcccg aatggaatcc ggcgagatcg tggtcatcct cgccgacggg ggttggaagt
     4981 acctgtcgac ggcgctgtgg acgatggact tcagcgatct gcccgaagac atcgacagca
     5041 aggtctggtg gtaggctcgg tgccgttgac actgccgcgt tcatacgtcg atgagatcgt
     5101 cgcacatgcg caagaggaga acccgaacga gtgttgcgga gtgattgccg gagacaacgg
     5161 ggttgcgatg aggctattca aagccgtcaa cgcggagcac agcccctacc gctacaacat
     5221 cgattcgcgc gaactcctga aaatttaccg ggagatagac gacaacggct gggagatgat
     5281 ggtgatctat cactcccaca cgcatacgcc ggcatacccc agtccgaccg atctgaacct
     5341 tgccggttat ccagacgcct attacctgct ggtatcgctt acatgggatc ctcccgacat
     5401 tcgtgcctat cgaattatcg acggagcggt tagccaagag gcactttttg tcgagtaaac
     5461 tgaacggact cacaatacag ggttccaacg cggggggagc aatggtcaac gaggtccacg
     5521 gccgcctcaa tattcccgtc cagcccatcc caaagcagac ggcagaggaa cgggtaaaga
     5581 attgggacga agtttatcgc ggtttcgacc ttgaatcggc gatcatcgag gcgaaccg
//
LOCUS       DATCPE010000045         3063 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U7219
            k141_590932, whole genome shotgun sequence.
ACCESSION   DATCPE010000045 DATCPE010000000
VERSION     DATCPE010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955033
            Sequence Read Archive: SRR1586279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 16:49:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 640
            CDSs (total)                      :: 631
            Genes (coding)                    :: 627
            CDSs (with protein)               :: 627
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3063
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_590932"
                     /isolate="SMAG_U7219"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            72..566
                     /locus_tag="VIM45_01400"
     CDS             72..566
                     /locus_tag="VIM45_01400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8819974.1"
                     /translation="MTTLKPEDLTPLERTYLGVLATGLVPADLARDTRFRMEFITAVC
                     HAMVEGLPEGGFLGDEEFTASTEFRHRLRKAIVDLDLKQVIGVGAPQDVVIMKPSVTQ
                     PEALYGQIDLNRHPPIFDRYLSQRCMDELMELPSVHLYLMALYADSGDIWHELYKQGY
                     GQYR"
     gene            563..1351
                     /locus_tag="VIM45_01405"
     CDS             563..1351
                     /locus_tag="VIM45_01405"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEY8819975.1"
                     /translation="MTATQPPENTGPVHPGVTIAGHYSSLVLPEPPPGRPFVYINMVT
                     SVDGKATVDGSERGLGSPDDQRMMHELRVHADAVMNGASTLRISGSSPRVRDRGLKAQ
                     RIQRGLAPQALGVVVTRSGDLPLDAPFFTLKDYDAVVVVVDTTPQERVSAISATGRHI
                     ITVPDRPDNGAEIVRALRDRFGVRHLLCEGGPTLNQTLLLAEVADELFLTVAPWIVAG
                     KENLTAAEGAPFHRLTMPRLRLLQYFHDQATDELFLRYAISYRG"
     gene            1384..1731
                     /locus_tag="VIM45_01410"
     CDS             1384..1731
                     /locus_tag="VIM45_01410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8819976.1"
                     /translation="MGVCRDTQCGGWLDVVSCVANGLRYHNLHNEFITWEAETMWYTL
                     VRFKDAPQHITGVSQAKTAADALLLMESWEQGFPGETTVVFDPKNAPVNRRALSDSAT
                     RIANANESWVRAR"
     gene            complement(1900..2478)
                     /locus_tag="VIM45_01415"
     CDS             complement(1900..2478)
                     /locus_tag="VIM45_01415"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004671 - protein C-terminal
                     S-isoprenylcysteine carboxyl O-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006481 - C-terminal protein methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEY8819977.1"
                     /translation="MPPWRRPFALLVLVVAIQRFYELVISRRNERRSGTPLGTGGKRS
                     FPLMVLLHMALFWMPLVERRLRPPRPRLAIAGPALVVCALATALRLWVIRSLGIHWNV
                     RGTVHAGTKVVVIGPYRYLRHPNYVAAALEMAALPLAGSAPISAALLSAANALVLIPR
                     VRGEEALLDAIPGYRERMGEKPRFIPRLRPRQ"
     gene            complement(2483..>3063)
                     /locus_tag="VIM45_01420"
     CDS             complement(2483..>3063)
                     /locus_tag="VIM45_01420"
                     /inference="COORDINATES: protein motif:HMM:NF014815.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HEY8819978.1"
                     /translation="GQKALIVAAEFPSLTFQPADDSMDNLVSTLVFADGVGAAVLSED
                     DSPGIEIVSSRSLLLPDSLTEMGFDLRDGGFHVVLSKDVPGLLAGPFGRATDQLLADY
                     GLTASRLAFACIHPGGPKILRAVDEALGVKGLTRPSWSVLSKFGNQSSAAVFFILSEL
                     LACGAPKSGAFGLMAGFGPGLSLELCLLQQRP"
BASE COUNT          620 a          956 c          953 g          534 t
ORIGIN      
        1 cgaacagcgg tcgatgcgat cggggtgagg ggccaaccgg ttcgctatgg tagcttgggg
       61 cgggaggcgc gatgacgacg ctcaagcccg aagatttgac ccccctggag cgcacgtacc
      121 tgggcgtgct cgccaccggg ctggttcccg ccgacctggc gcgcgacact cgcttccgca
      181 tggaattcat cacggcggtc tgccatgcaa tggttgaggg tctgccggag ggcggatttc
      241 tcggcgacga ggaatttacg gcgtcaacag aattccgtca taggctgcgc aaagcgatcg
      301 tcgacctcga tctcaagcag gtgatcggag tcggcgcccc gcaggacgtt gtgatcatga
      361 agccgtcggt aacgcagccc gaggcgctgt acgggcagat cgacctcaac cgtcacccac
      421 caatcttcga tcgttacctt tctcaacggt gtatggacga actgatggag ctgccgtccg
      481 ttcacctcta cctgatggct ctttacgccg acagtggcga catctggcac gagctttaca
      541 aacagggata cggccaatac cgatgaccgc aacacagccg cccgaaaaca cgggaccggt
      601 gcatccgggc gtcaccattg ccgggcacta cagtagcctc gtactgcccg aaccgccgcc
      661 tggccgccca ttcgtctaca tcaacatggt cacgtcagta gacggcaagg cgacagtgga
      721 cggctcggaa cgcggcctcg gcagtcccga cgaccagcgc atgatgcacg agctgcgggt
      781 gcacgcggac gccgtcatga acggagcgag caccctgcgc atcagcggtt cgtcgcctcg
      841 cgtgcgcgac agagggctaa aggcgcaacg gatacaacgg ggtctcgctc ctcaggcgct
      901 gggtgtcgtc gtaacgcgat ccggagacct gccgcttgac gccccttttt tcacgttgaa
      961 ggattacgac gcggttgtgg tggttgtgga taccacgccg caggagcggg tgtcggcgat
     1021 cagcgccacg gggcggcaca taatcacggt ccccgacagg cccgataacg gcgctgaaat
     1081 cgtgcgcgcg ttgcgcgatc ggttcggtgt gcgacatctt ttgtgcgaag ggggacccac
     1141 gctcaaccag acgctcctac tcgccgaagt ggctgacgaa ctgttcttga cggtggctcc
     1201 atggatcgtg gccggaaagg agaacctgac cgccgccgag ggtgctccgt tccatcgctt
     1261 gacgatgccc cgtctccggc ttttgcagta ctttcacgat caggcaaccg acgagctttt
     1321 tctccgctac gcgatctcgt atcgaggtta ggcgcagaac actggtaacc tgttgcgatc
     1381 caggtgggcg tatgccgcga tactcaatgc ggcggctggc tcgacgtggt aagctgcgtc
     1441 gcgaacggtc tccgatacca caatctgcac aacgaattca ttacctggga ggcagaaacc
     1501 atgtggtaca ccctcgtccg cttcaaggat gctcctcagc acatcaccgg agtctcgcag
     1561 gcgaaaacgg cagccgatgc gctcctgttg atggaaagct gggaacaggg cttccccggt
     1621 gagaccacgg tggtgttcga tcccaagaat gctcccgtca accgaagggc gctttcggat
     1681 tcagccacgc gtatcgccaa cgccaacgag tcgtgggtcc gcgcccgcta gcattgatta
     1741 ctgcggctat tcggccgtgg cagtatgccg gtcgaaagta ctcccattgg gcgatttaac
     1801 gcccgccgtc acgatagcca tactctccct gtaagctcac ggttcggcgg ggtgccgtga
     1861 gatgacagca aacgggagcg gcatagccgc ccccgtttgt cattgcctgg ggcgcaggcg
     1921 agggatgaag cgcggcttct ctcccatccg ttcccgatag ccgggaatgg cgtcgagaag
     1981 cgcctcctcg ccgcgcacgc ggggaataag caccagagcg ttggcggcgc tgagcagtgc
     2041 cgccgatatc ggcgctgagc cggccagcgg aagcgccgcc atctccagcg ctgcggccac
     2101 gtagttcgga tggcgaaggt agcgataggg tccgatgacg acgaccttcg tgccagcgtg
     2161 aactgtccct cgcacgttcc agtgaatccc cagagaacga atcacccata gccgcagcgc
     2221 cgtcgcgagt gcgcagacga ccagtgcggg acctgcgatc gcgagacgcg gccttggcgg
     2281 acgcaatcgc ctctcgacca gcggcatcca gaagagtgcc atatgcagca gcaccatcaa
     2341 cgggaagctt cgttttccgc cggtgcccag cggtgtccca gatcgtcgtt cattgcgccg
     2401 agagatcacc agctcgtaaa agcgttggat agcaacgacg agcacgagca gcgcaaacgg
     2461 gcgacgccac ggcggcatca catcagggcc gttgctgcag caggcaaagc tcaagggaga
     2521 gacccggacc aaagcctgcc atcaggccga acgcgccgga tttcggcgcg ccgcaggcta
     2581 acagctccga aaggatgaaa aagaccgcag ccgacgactg gttgccgaac ttcgagagca
     2641 ccgaccagga cggacgcgtc agacccttta ctcctagcgc ctcatctacc gcccgcaaga
     2701 tcttcgggcc gccggggtga atgcacgcga aggcgagtct ggacgccgtt agtccataat
     2761 cggcgagaag ttggtccgtc gctcgtccaa acggtccggc cagtagcccg ggcacgtcct
     2821 tgctcaaaac gacgtgaaag ccgccgtcgc gcaggtcgaa ccccatctcg gtcagcgaat
     2881 ccggtagcag caggctgcgg ctggagacaa tctcgatccc gggactgtcg tcttcggaca
     2941 gcaccgcggc cccgactccg tcggcgaaga ccagtgttga gacaaggtta tccattgagt
     3001 cgtcggcggg ctgaaaagtg agcgaaggga actcggccgc tactatcaga gccttttggc
     3061 cgc
//
LOCUS       DATCPE010000046        14014 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U7219
            k141_591255, whole genome shotgun sequence.
ACCESSION   DATCPE010000046 DATCPE010000000
VERSION     DATCPE010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955033
            Sequence Read Archive: SRR1586279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 14014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 16:49:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 640
            CDSs (total)                      :: 631
            Genes (coding)                    :: 627
            CDSs (with protein)               :: 627
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14014
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_591255"
                     /isolate="SMAG_U7219"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="VIM45_01425"
     CDS             complement(<1..375)
                     /locus_tag="VIM45_01425"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEY8819979.1"
                     /translation="MPGGRGILLVRIELNFRNLLLLFALLVVAWVTYLVRDTIVLLGL
                     ALMLMATLHPLVQMAERRGLSHSWAVALVMLALVLVPVAMIAAMTPIIISEVQSFAKS
                     FPTLQQHINELLHNWGVADRVNQ"
     gene            complement(435..1322)
                     /locus_tag="VIM45_01430"
     CDS             complement(435..1322)
                     /locus_tag="VIM45_01430"
                     /inference="COORDINATES: protein motif:HMM:NF006128.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HEY8819980.1"
                     /translation="MVEFENLIYEKENGRARITLNRPEKLNALSIALQRELNAAMREA
                     DNDTDVHCVILRGAGRAFSAGYDLTAGGRRNSDETYANIYRGRVSLDDDIWQMEQSQQ
                     LRMIIFDMHKPVIAQVHGYCLAGGTDVALLCDMIIAADDAQLGFPPVRAMGAPPAHMW
                     TYMVGPQWAKRLLLTGDTISGADAERIGLVLQAVPAIELEQTVEALADKLAMIDSELL
                     SANKRVCNMALELMGARTMQRFAAEMDGRAHLAPSVQEFGRISREQGLKPALEWRDSK
                     FGDGRASAAAVARKRAATT"
     gene            complement(1377..2129)
                     /locus_tag="VIM45_01435"
     CDS             complement(1377..2129)
                     /locus_tag="VIM45_01435"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY8819981.1"
                     /translation="MTDGLLELAGKRALVIGAGTPAGRAAAVALAAAGADVAVASGSI
                     DGDEVMAVRRARRAIEALGRRTAEYAFDLTLGANVRVSTRQVAKEIGGLDILVNAADV
                     YLRRSTESTSDSDWSRVLNVNLNGVFYACRSALKEMEEHGGAIVNVSSVLGERGAVES
                     AAYCAARHGVIGLTRALALEYAGRNIRVNAVALGWLQGVPQDISPLPPASMPEKSDAV
                     QSVGPLVVYLVSAASGFVNGQVFTLDGSPVAV"
     gene            2367..2519
                     /locus_tag="VIM45_01440"
     CDS             2367..2519
                     /locus_tag="VIM45_01440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8819982.1"
                     /translation="MGKLFKVGILAGIIGGVVYAWKRMTGGSAQESDSAGGNEAPARR
                     DGDHKS"
     gene            2561..4231
                     /locus_tag="VIM45_01445"
     CDS             2561..4231
                     /locus_tag="VIM45_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEY8819983.1"
                     /translation="MLYDARTLKELAGRRAAQEAEVAGGSEAAGPSVTTSGRPIQRLY
                     SPVDQPDFDYQKDLGNPGEYPYTRGIHPTGYRGRKWTIRMFAGFGSAEETNARYRYLL
                     DHGETGLSVAFDMPTLMGYDTDDEWGLGEFGKCGVAISSLADMELLFAGIPLDQITTS
                     MTINSPAAVIWAMYIAAAEKRGVPRAALGGTLQNDILKEYIAQNEFIYPLAESMRLVT
                     DTIEFGSQEMPKWNTVSVSGYHIREAGATAVQELAFTLADGIEYTKWALARGLDIDAF
                     APRLSFFFNAHNDFFEEICKYRAARRVWAKVMRERFGAKNPRSWWLRFHTQTAGVSLT
                     EQQPEVNLIRTTIQALSAVLGGTQSLHTNSWDEAMALPSEKAVRLAVRTQQVIAHESG
                     VSNTVDPLGGSYFVERLTSEMERECFAYFDQIDALGGVLPAIESGFFQREIADSSYRY
                     QREIDRHERTIVGVNDFQLEEPVDIPILRMDPDGEGRHLARLNRIRCERDQGCWSGVM
                     SQLEAVCRDGKTNTMPALIAAVNAYATLGEICGVMRKVFGEYRELVVV"
     gene            complement(4293..4370)
                     /locus_tag="VIM45_01450"
     tRNA            complement(4293..4370)
                     /locus_tag="VIM45_01450"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4333..4335),aa:Pro,seq:cgg)
     gene            complement(4451..4654)
                     /locus_tag="VIM45_01455"
     CDS             complement(4451..4654)
                     /locus_tag="VIM45_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8819984.1"
                     /translation="MVFPKCQKCKLGDLVPLSDFGSQGAAIHFKAWACTNPDCGFNIK
                     IRNGDLYIDEPITSGALHSPRFR"
     gene            4899..5612
                     /locus_tag="VIM45_01460"
     CDS             4899..5612
                     /locus_tag="VIM45_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008880853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY8819985.1"
                     /translation="MANTVLVVDDEKNIVELARVYLRNAGFQVEAAYDGLEAIEKTRS
                     LKPAIVVLDLMLPELDGMEVCRAIRKENEVPIIMLTARSDDMDKIIGLEIGADDYLTK
                     PFNPRELVARIKAVLRRSEAGKRPPKVIEVGDMRIDVLGREASVAGVPVPLRTKEFDL
                     MLALANNVGIAMARDRILNLVWGEDFFGDQRTLDVHVAWLRDKISASTAKIVTVWGFG
                     YKLIAGEPAKAAAVESKRA"
     gene            5649..7118
                     /locus_tag="VIM45_01465"
     CDS             5649..7118
                     /locus_tag="VIM45_01465"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY8819986.1"
                     /translation="MFRTLRIKLAVAFAMITLLSIVAVGAGSLFLLRDHEVKAATQRT
                     GDLLPPEAQEIGMFASRGVSQARIDGYLRQRADQLHVRFILLDGDSQVLLDTDGKQSG
                     TFTKLAAGKSGRNLPNSYHVTAYQEGPEHYVLFTQPSGALTLQGQRTVIPTQFNLLML
                     VPETSITSAWTDLAPRLIMAGGVALVASLGLALLIARSISRPLAGITRASEAMAQGNY
                     EQQIPVKGRDEIARLARSFNGMAAQVSGSDRMMRDLLANVAHELKTPLTSIQGFSQAL
                     ADGTLQTPDEHSTAARIINDESERMRRLVNDLLYLSQIESGQIPLELQPTDVGMLLNE
                     SAERVHWQLSGSDKRMITESAERLPPVSADPRRMEQVIANLLDNAMRYTPDGGEVRLS
                     ATQFDEGHVRIDVHNSGSYIAPVDLDRIFERFYQVDRSRRRNGHNGGLGLAIARQIVT
                     ALGGQISARSDPKTGTTFSVSLPIASGAPYAKVPAAAAD"
     gene            complement(7237..8844)
                     /gene="cimA"
                     /locus_tag="VIM45_01470"
     CDS             complement(7237..8844)
                     /gene="cimA"
                     /locus_tag="VIM45_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HEY8819987.1"
                     /translation="MPGVVQLYDSTLRDGSQMEGISFSLEDKLLIAQKLDEIGIHFIE
                     GGFPGSNPKDAEFFVRARDLELRQARIVAFGGTRRAGLSCDEDPNIQSLVHAHTQAIC
                     FVGKASARQVRDILETTPEENLSMIADSVAYMRAIGKQVFFDAEHFFDGFFDDPGYSL
                     QVLRTAHRAGAEVAVLCDTNGGTITPKLLEAIGAVRETGVPFGIHVHDDTGLGVANTL
                     AAFEAGAVQLQGCLNGYGERCGNANLTTVIANLKVKLGVDCVSDEQLSRLTEVSHFVS
                     ELANLAPNSQAPYVGQSAFAHKAGYHVAAIVKDASSYQHIDPVTVGNDRRVLISELSG
                     QRNILAKLEEQGVHIPLNGEETKRLLQSVKTMESRGYQFEGAEASFEVLVRRGQPDYT
                     PPFELEDFLIVERRRHKADRDGETKEMLAEAMAKVRVGEEVRHTAAEGNGPVNALDGA
                     VRKALVEFFPDLERIRLLDYKVRILDGNTGTSASTRVLIESTDGVQIWRTVGVSTDII
                     EASWLALADSLEYWLYHHTRRHAQMLA"
     gene            complement(9027..10124)
                     /gene="leuB"
                     /locus_tag="VIM45_01475"
     CDS             complement(9027..10124)
                     /gene="leuB"
                     /locus_tag="VIM45_01475"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011309304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEY8819988.1"
                     /translation="MTTFKIAVLPGDGIGPEVVAEGTRVLKAVERRFGHRFEFNEAIV
                     GGAAIDKYGVALLPETLDMCKASDAVLFGAVGGPKWDDPRAKVRPEQGLLGLRKGLGL
                     FANLRPVKVHPLMVNSSTLKSEVLDGTDIIIIRELTGGLYFGQPKRRWENATGRQSVD
                     TNRYSEKEVRRVLQVGFDLARQRRKKLTSVDKANVLETGRMWREIAIEMGKQNPDIEL
                     DHILVDACAMHLIRHPSTFDVIVTENTFGDILTDEASMLVGSMGMLPSASIGKTRKSG
                     TGHGLYEPIHGTAPDIAGQGKANPLATILSVAMMLRLSLGLEAEAQTVEKAVDSALQQ
                     NLRTPDIAQEGTTMVNTSQMGDAVAKAVLAP"
     gene            complement(10379..11932)
                     /locus_tag="VIM45_01480"
     CDS             complement(10379..11932)
                     /locus_tag="VIM45_01480"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011142287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEY8819989.1"
                     /translation="MPDRVIIFDTTLRDGEQAAGVALNGDDKMQIAKQLERLGVDVIE
                     AGFAASSPGDFDAVMAIAREIKGATVASLARAVPGDVDAAWEALRNATEKPRIHVFLS
                     SSDIHIMHQLQKDYEQVLNQAAEMVARAKGYTSDIEFSPMDATRSRPEYVWKMLEAVI
                     DAGATTVNIPDTVGYAIPEEFEQFIRGIFQNVRNIDKATVSVHCHNDLGLSTANSLAA
                     LRAGARQIEVCVNGIGERAGNAALEEVVMSIKTREDLFDVGMGIDTTQLYRTSKLVEH
                     LTGMPVQHNKAIVGKNSFRHQSGIHQDGILKLRATYEIMDPKSIGVPEGTQLVLGKLS
                     GRHALQQRLEDMGYQLNNEELSRAFLAFKELADKKVEVDDRDLEALLMDEKRVASEVY
                     HLIWVQVQSGSNMTATGTVRIRLPNGDEATDAAIGDGPVDAAFKAVDRIVGMEAELLD
                     YHVKSVTEGTDAQGEVVLRVKSGEHVATGRGADTDIIVASAKAYLDALSRAGHLAKVA
                     NRVPTASTP"
     gene            complement(12235..13710)
                     /locus_tag="VIM45_01485"
     CDS             complement(12235..13710)
                     /locus_tag="VIM45_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013163332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEY8819990.1"
                     /translation="MPATFTTETFEKEPLRLGSTEEFIVRGGRSLFPRLADAFEGIRQ
                     IGVLGWSSQAPAQAQNLRDSLAGTAITVKIGLREGSASIPAAERAGFTRASGTLGEMY
                     QVIAESDLVLLLISDAAQAEEYQRIFAALRPGSTLGLSHGFLLGHLKNVGASFPENVN
                     IIGVCPKGMGPSVRRLYEQGADVDGAGINSSFAIQQDVTGRATDYALGWAVGLGSPFA
                     FQTTLESEYKSDIFGERGILLGAVHGIVECLYRWFVQHGASQEEAFINSVESVTGPIS
                     KGISRYGILGVFNMLGDADKETFLKAYSAAYPAAFDVLLEIYDEVSSGNEIRSVVMAQ
                     ERSERFPMGKIDGTEMWQVGEGARARRQTLKTPIHPLTAGVYVATMMAQVDLLREKGH
                     PYSEIANESIIEAVDSLNPYMHFKGVAYMVDNCSTTARLGARKWAPRFDYIITQQALT
                     AIDRDEPVNERLRTEFLNNEIHQVLSKCSELRPPVDIAILE"
     gene            complement(13762..>14014)
                     /locus_tag="VIM45_01490"
     CDS             complement(13762..>14014)
                     /locus_tag="VIM45_01490"
                     /inference="COORDINATES: protein motif:HMM:NF021855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8819991.1"
                     /translation="FRAEIIDVTDTTLVVQAVGDEETLESLLQVLRPYGIREIVRTGR
                     VALTRGATSTTVDVEPAEIRQFHRREGRMPNKPLPFVSD"
BASE COUNT         2561 a         4439 c         4314 g         2700 t
ORIGIN      
        1 ctggttaacc cggtccgcga caccccagtt gtgtagcagc tcgttgatgt gctgttgcag
       61 ggtcgggaag ctcttagcga aactttgaac ctcggaaatg atgatcggcg tcatggcggc
      121 tatcatcgcc accggcacga gcacgagcgc cagcatgacc aacgcgacgg cccaactgtg
      181 cgacagaccc cgccgttcgg ccatttggac gagcgggtgc agcgtcgcca tcagcatgag
      241 cgcgaggccg agaagcacga tcgtatcgcg caccaggtac gtgacccacg ccactaccag
      301 gagtgcgaac aatagcaata ggttgcgaaa gttcagctcg atccgaacca gtagaatacc
      361 cctcccgccc ggcaccgtgc gccgggaatt gccgcgccgg cgcaggtcgt gccgcgctca
      421 ttgttgaggc tcttttacgt ggtcgctgcc cgcttgcgcg ccacggccgc ggcgcttgcc
      481 cgtccgtcgc cgaacttcga gtctcgccat tcgagcgcgg gcttcagacc ttgctcgcgg
      541 gagatccggc cgaactcctg tacgctcggc gccaggtggg cgcgtccatc catctccgcg
      601 gcgaaccgct gcatggtacg ggcgcccatt agttccagcg ccatgttgca gacgcgcttg
      661 tttgcggaca gcaactcgct gtcgatcatc gccaatttat cggccagtgc ttcgacggtc
      721 tgttcgagct caatcgcggg gacggcctgt aaaacgagtc ctatcctttc ggcgtcggcg
      781 ccggagatcg tatctcccgt gagcagcagt cgcttcgccc attgaggtcc aaccatgtag
      841 gtccacatgt gagccggcgg agcgcccatg gcgcgtaccg gcggaaagcc gagctgcgca
      901 tcgtcggccg cgatgatcat gtcgcacaac agcgccacgt cggtgcctcc ggcaaggcag
      961 tagccgtgca cctgtgcgat gaccggttta tgcatgtcga aaatgatcat gcgtagctgc
     1021 tgtgactgct ccatctgcca gatatcgtcg tcgagcgata cgcggcctcg gtagatgttg
     1081 gcgtaggtct cgtcgctgtt tcggcgaccg ccggcagtga ggtcatagcc ggcggagaat
     1141 gcgcgtccag cgccgcgcaa gatgacgcaa tggacatctg tgtcgttgtc ggcttcacgc
     1201 atcgccgcgt ttaattcgcg ctgcaaggcg atcgaaagcg cgttcagttt ctccgggcgg
     1261 ttgagcgtga tccgggcgcg gccgttttct ttctcgtaga tcaggttctc gaactctacc
     1321 atggttcgtg cctccctctc tgcacttttc cctggccgac caacgtttcg atatcgctaa
     1381 acggcgaccg gcgagccgtc cagcgtaaat acctgaccat tcacgaagcc gctcgccgca
     1441 gacaccaggt acaccacgag cgggccaacg ctctggacag catccgattt ctccggcatc
     1501 gatgccggcg ggagaggcga gatatcctgc ggcacgccct gcagccagcc gagtgccacc
     1561 gcgttcaccc ggatgttgcg gccggcatat tccagggcaa gcgcccgggt aagcccaatc
     1621 acgccgtggc gagcagcgca gtacgcagcg ctttccacgg cgccgcgctc ccccagtact
     1681 gaggaaacgt tgacgatcgc accaccgtgt tcttccatct ccttgagggc ggaccggcac
     1741 gcgtaaaata cgccgttcaa gttgacgttt aatacccgtg accaatccga atcggaagta
     1801 ctctcggtgc tacgccgcag gtagacgtca gcggcgttga cgagaatgtc cagcccgcca
     1861 atctccttcg cgacctgccg ggtcgacacg cgcacattgg cgccgagcgt caggtcaaac
     1921 gcgtattcgg ccgtgcgccg tccgagcgcc tcgatcgcgc ggcgcgctcg tcggaccgcc
     1981 atcacctcgt cgccgtcgat cgacccggac gcgacagcca catcggcccc ggcggcggcc
     2041 agcgcgaccg cagcggcgcg gccagcgggc gttcccgcgc cgatcaccag cgcccgttta
     2101 ccggccagct ccagtagacc atcggtcatc ggcacgccgc tcctggcttc gaccttcgga
     2161 gaatggataa tgtgaacttc gagaccattg cgcgcatgat accgctgcac gggcaccggc
     2221 ggacagccgg gcaatcaagg tggatttttg cgattagagc gagaacggtt cagcgatgat
     2281 caccaagtat ccgaggggct cggcctgcat gatacgataa tcgcacctgc gaataacccg
     2341 cagttcgcgc gaatggaggc gttctaatgg gcaagctctt taaggtcggc attctagctg
     2401 gcatcattgg cggcgtcgtc tacgcctgga agcgtatgac cggcggcagc gcccaggaaa
     2461 gcgattcggc cggcggcaac gaagcccccg cccgacggga tggtgatcac aagagctgac
     2521 ccgcgctagc cccacccgaa atgtctcagg gagtgagacg atgctctacg acgcgcgaac
     2581 tctgaaagag ctggccgggc ggcgcgccgc ccaggaggcg gaggtcgccg gcggcagtga
     2641 agccgccggg ccgtccgtca cgacttccgg ccgccccatt caacgcctct acagccccgt
     2701 ggaccagccc gacttcgact accaaaaaga tcttggtaac cccggcgagt acccctatac
     2761 acgcggcatc caccccaccg gctaccgcgg gcgcaagtgg acgatccgca tgttcgccgg
     2821 ctttggctcg gcggaggaga cgaacgcccg ctaccgctac ctcctggacc atggtgagac
     2881 cggtctctcg gtcgcgttcg acatgccgac gctgatgggt tacgacaccg acgacgaatg
     2941 gggcctcggc gagttcggca agtgcggcgt tgccatctcc agcctggcgg acatggagct
     3001 cctgttcgcc ggcatcccgc tggaccagat cacgacctca atgacgatca actccccggc
     3061 ggcggtgatc tgggcgatgt acatcgccgc tgcagagaag cgcggggtac cgcgggcagc
     3121 gttgggcggt acgctgcaga acgacatcct caaggagtat atcgcccaga acgagttcat
     3181 ctacccgctg gccgagtcga tgcggctggt gaccgacacg atcgagttcg gctcgcagga
     3241 gatgccgaag tggaacacgg tctcggtcag cggctaccac atccgcgagg ccggggcgac
     3301 ggcggtgcag gagctggcgt ttacgcttgc cgacggcatc gagtacacga agtgggcgct
     3361 ggcacggggc cttgacatcg acgcctttgc gccgcggctc tccttcttct tcaacgcgca
     3421 caacgacttc ttcgaggaga tctgcaagta ccgcgccgcg cggcgggtgt gggccaaggt
     3481 catgcgcgag cgcttcgggg ccaagaaccc gcgctcatgg tggctgcgct tccacacgca
     3541 gacggcaggc gtctcgctca ccgagcaaca gccggaagtc aacctgatcc gtacgaccat
     3601 ccaggcgctg tcagcggtct tgggcgggac gcagagcctg cacaccaact cgtgggacga
     3661 agccatggcg ctgccgagcg aaaaggcggt gcggctggcg gtgcgcacgc agcaggtgat
     3721 cgcccacgag tcgggcgtga gcaacaccgt ggaccctctg ggcggtagct attttgtcga
     3781 gcggctcacc agcgagatgg agcgcgagtg cttcgcctac ttcgaccaga tcgacgccct
     3841 gggcggggtg cttcccgcga tcgagagcgg gttcttccag cgggagatcg ccgactcgtc
     3901 gtaccgctac cagcgggaga tcgaccgcca cgagcggacg atcgtggggg tgaacgactt
     3961 ccagctcgag gagccggtgg acatccccat cctgcgcatg gacccagacg gcgaggggcg
     4021 ccacctggcg cgactgaatc gcatccgatg cgagcgggat cagggctgct ggtccggggt
     4081 gatgtcgcag ttggaagcgg tgtgtcgcga cggcaagacg aacacgatgc cggcgctgat
     4141 tgcggcggtg aacgcctacg ccacgctggg cgagatctgc ggcgtgatgc gcaaggtctt
     4201 cggcgaatac cgcgagctgg tggtcgttta acgaccggcc cgcgttgcgg actgcctcga
     4261 caaaagaaaa ccggcccgcg cgggccggtg tctggtcggg gtgggcggat ttgaactgcc
     4321 ggcctctgcg tcccgaacgc agcgctctaa ccaagctgag ctacaccccg gtggaaccag
     4381 tgaggcgcgc gaagcctcaa ccatcattcg ctgtaatacc gtatcagccc aaaacgcatt
     4441 ctgtccagaa ctagcggaag cgcggactgt gaagcgcacc gctcgtgatc ggctcatcga
     4501 tatagagatc gccgtttcgg atcttgatgt tgaagccgca gtccgggtta gtgcatgccc
     4561 atgctttgaa gtgaattgca gcaccctggc tgccgaagtc ggagagcggt acgagatccc
     4621 caagcttgca cttttggcac tttggaaaga ccatctccac cacccacctc cctcgtccgt
     4681 acgaatacgc cgggcgccga cgactgccac aacggaaaat tcggcttcgc agccattgct
     4741 agttaacaaa tgtttaacac attcgcggga cccggtccac ggtctacact ggtacttggg
     4801 cagcggcgaa ttaatttagc atttcgttta cagcggcttt accggggaat ggctttcaag
     4861 cgcttaaatg gatgatgaag gccccgagga ggacgtccgt ggcaaacacc gtcctggttg
     4921 tcgatgacga aaagaatatc gttgagctcg cgcgcgtcta cttacgcaac gcaggcttcc
     4981 aggtcgaagc agcctacgac ggcctggaag ccatcgagaa gacgcggtct ctaaagccgg
     5041 ctatcgtcgt gctggacttg atgttgcccg agctggatgg tatggaggta tgccgcgcga
     5101 ttcgtaagga gaacgaagta ccgatcatca tgctcacggc ccgcagcgac gacatggaca
     5161 agattatcgg gctcgaaatc ggcgccgacg attacctcac caagcccttc aacccccgag
     5221 agttggtggc gcgcatcaaa gcagtgctgc gtcgcagtga ggccggcaaa cgcccgccca
     5281 aagtaataga ggtcggcgac atgcggatcg acgtgctcgg tcgagaggcg agcgtcgccg
     5341 gagtgccagt gccgctgcga acgaaagaat tcgacctgat gctggcactc gctaacaacg
     5401 tgggcatagc gatggcccgt gaccgcatcc tgaaccttgt ttggggcgag gacttttttg
     5461 gcgatcagcg cacgctggat gtccacgttg cctggctgcg cgataagatc agcgccagca
     5521 cggcgaagat tgtcacagta tggggctttg gttacaaact gatcgctggc gagccggcga
     5581 aggccgccgc ggtcgaatcc aaaagggcgt agcgttccta tcggtctcgc gacgggccat
     5641 ggacggtcat gtttagaacc cttcgaatca agcttgcggt cgccttcgcc atgatcacgc
     5701 tgctgtcgat cgtggcagtc ggcgccggct cactattctt gttgcgcgac catgaggtca
     5761 aggcggccac gcagcgtacc ggcgatctgc tgccccctga agcgcaggag atcggcatgt
     5821 tcgcgagccg aggtgtatcc caggcgcgaa tcgacggcta cctacgtcaa cgcgcggatc
     5881 agctccacgt gcgcttcatc ctcctcgacg gggattccca ggtgctcctt gataccgatg
     5941 gcaaacagag cggcaccttc acgaaacttg ccgccggcaa gagcggtcgc aatcttccca
     6001 acagctacca cgtaacggcc taccaggaag gtcccgagca ctacgttctc ttcactcagc
     6061 ccagtggggc gcttaccctc cagggtcaac gtaccgtcat accgacgcaa ttcaacctgt
     6121 tgatgctcgt tccggagacg agcattacgt cggcctggac ggacctcgct ccgaggctga
     6181 tcatggccgg gggggtggcg ctggttgcgt cgctagggct cgcgttgctg atcgcacgaa
     6241 gcatctcccg cccgctggcc ggcatcacga gggcctcgga ggcgatggct cagggtaact
     6301 acgaacagca aattccggtg aagggaagag atgagatcgc ccggctcgcg cgctcattca
     6361 acgggatggc cgcgcaggta tcggggtccg atcgtatgat gcgggatctc ctggcgaacg
     6421 ttgcccacga gctcaagacg ccgctgacgt cgattcaggg cttttcacaa gcgcttgccg
     6481 atggcacgct gcagacgccc gacgaacact cgactgccgc ccgcattatc aacgatgaat
     6541 cggagcgtat gcggaggttg gtgaacgacc tgctctacct ttcccagatc gaatcagggc
     6601 agatcccgct ggagcttcag cccacggacg tcggcatgct gctgaacgaa tctgctgagc
     6661 gagttcactg gcagttgagc ggcagcgaca agcggatgat taccgaatcg gcggaaaggc
     6721 tgcccccggt gagcgccgac ccccgacgca tggaacaggt gatcgcgaac ctgctggaca
     6781 atgcgatgcg ttatacgccg gacggcggcg aggttcgcct gagcgccacc cagtttgacg
     6841 agggtcacgt ccgcatcgac gtacacaaca gtggctcata tatcgctcct gtcgacctcg
     6901 accgcatatt tgagcgcttc taccaggtcg atcggtccag acgccgcaac ggacacaacg
     6961 gcggcctggg actggcgatt gcccgacaga tcgttacggc gctcggcggt cagatatcgg
     7021 cgcggtccga cccgaaaacg ggtacgactt tcagcgtatc ccttcctatc gcgtcgggcg
     7081 caccgtatgc gaaagtgccg gcggctgcag ccgactaaac ggtcagcatc aggcgcttcc
     7141 gccggacgcg ttgcaactca gtccgaatcg tgcttccagt gatcgtccct tccctcctca
     7201 gggaagggag acgagggtta ggagcgctgg cttaccctat gccagcatct gcgcgtgacg
     7261 gcgagtgtga tggtagagcc agtattcaag cgagtcggcc agcgccagcc agcttgcttc
     7321 gatgatgtcg gtcgaaacgc cgaccgtgcg ccagatctgt acgccatcgg ttgactcgat
     7381 cagcacgcgc gtcgaggccg aagtgccggt gttgccatca aggatgcgca ccttgtagtc
     7441 gagcaggcgg atgcgctcca ggtcgggaaa gaactcgacc agcgccttgc gcaccgcgcc
     7501 gtccagcgcg ttcacgggac cgttgccctc ggcggccgtg tgccgaactt cttcgccgac
     7561 gcgtaccttt gccatcgcct cggccagcat ctccttcgtc tcgccgtcgc ggtccgcctt
     7621 atgacgtctc cgttcgacga tcaagaagtc ttcaagctca aacggcggcg tgtaatccgg
     7681 ctgaccgcgg cggacgagta cctcgaacga tgcctctgcg ccctcaaact ggtagccgcg
     7741 actctccatt gtcttgacgc tttggaggag ccgcttcgtc tcttcgccat tcagagggat
     7801 gtggacaccc tgctcttcca gcttggcaag gatattgcgc tgcccggaaa gctcggagat
     7861 gagcacgcgt cggtcgttgc caacggtcac cgggtcgatg tgctggtacg acgaggcgtc
     7921 cttgacgatc gccgccacgt ggtaacccgc cttgtgcgcg aatgcgctct ggccgacgta
     7981 cggcgcctgc gagttcggcg caagattggc cagctcgctg acgaaatgcg acacctcggt
     8041 tagtcgggaa agctgctcgt cgctgacgca gtcgacgccg agcttcactt tcaggttggc
     8101 gattaccgtt gtgaggttgg cgttgccgca gcgttcaccg tagccgttca gacagccttg
     8161 aagctgcact gcacccgctt cgaacgccgc gagcgtgttg gcgacgccga gacccgtgtc
     8221 gtcgtggaca tggatgccga aaggcacccc ggtttcacgc accgcgccga tcgcttccaa
     8281 gagttttggc gttatggttc cgccgttggt atcgcatagc acggccactt cggcgcccgc
     8341 tcggtgcgct gtgcgcagca cctgtagcga ataccccgga tcgtcgaaaa agccgtcgaa
     8401 gaagtgctcg gcgtcgaaga agacctgctt gccaatcgcc cgcatgtacg ccacggaatc
     8461 cgcgatcatg gacaggttct cttccggcgt cgtctccagg atatcgcgca cctggcgcgc
     8521 ggatgctttg ccgacgaaac agatcgcttg cgtatgcgcg tgcacgagcg actggatgtt
     8581 cgggtcctcg tcgcagctta agccggcgcg gcgggtgccg ccaaaggcga cgatgcgagc
     8641 ctgccgcagt tcgaggtccc gggctcggac gaagaactcg gcgtctttag ggttcgagcc
     8701 gggaaagccg ccttcgatga agtgaattcc gatctcgtcg agcttctgcg cgatcagcag
     8761 cttatcttcg agtgaaaacg agatcccctc catctgcgat ccgtcgcgga gggtcgaatc
     8821 atacagttgt acgacgcctg gcatggtgag cgagccccct aacccccagc cccttccgcc
     8881 ccaatactgg ggggaagggg cgatctctgg tacctgcgtg ctggcagtgt cggtactccc
     8941 gctttccgtc ctattccgat tcggacttca cgcctctgtc gtagctccct ctttccccta
     9001 gcattggggg aaagagggcc gcggagttag ggggccagca cggccttcgc aaccgcgtcg
     9061 cccatctgtg atgtgttgac catcgttgtt ccttcctgcg cgatgtcggg tgtacgaagg
     9121 ttttgctgga gggcggaatc gaccgctttc tcgacggtct gggcttcggc ttcaagtccc
     9181 agcgagagac gaagcatcat cgcgacgctg aggatcgtcg caagcgggtt cgccttgccc
     9241 tgtccggcga tatcgggcgc ggtgccatga attggctcgt agagaccgtg tcccgtgccg
     9301 cttttgcgcg tcttgccgat gctggctgac ggcaacattc ccatcgaccc caccagcatg
     9361 gacgcctcgt ctgtgaggat gtcgccgaac gtgttctcgg tgacgatgac gtcgaacgtg
     9421 gacgggtggc ggatgagatg catcgcgcag gcatcgacca ggatgtggtc aagttcgatg
     9481 tctggattct gcttgcccat ttcgatcgca atttcgcgcc acatccgtcc tgtttcgagg
     9541 acgttggctt tgtcaacgct ggtcagcttc ttgcggcgtt gacgcgcgag gtcgaaaccg
     9601 acctgcaaaa cgcgccgcac ttccttctcc gagtagcggt tggtatcgac cgactggcgg
     9661 ccggttgcgt tctcccagcg acgcttaggc tggccaaagt aaaggccgcc ggtgagctcg
     9721 cgaatgataa taatgtctgt gccgtccagc acttcgcttt tgagggtgct ggagttcacc
     9781 atgagcgggt gaacctttac cggtctcagg ttggcgaaca gcccgagccc tttgcgcagt
     9841 ccaagcagcc cttgttccgg ccgcaccttt gcgcgcgggt cgtcccactt gggaccgccc
     9901 acggcaccga acaagacggc gtcgctcgcc ttgcacatgt cgagcgtctc gggaagcagt
     9961 gcgacaccgt acttatcgat tgcggccccg ccgacgatcg cctcgttgaa ctcgaagcgg
    10021 tgtccgaagc gccgctccac ggcctttagc accctggtgc cctcggccac aacctcgggc
    10081 ccgatgccgt cgcccggaag aaccgcgatt ttgaacgttg tcacgctttc ctaacccctt
    10141 tcccgtccgg ccccctaacc cccgtcccct tccaccccat tactgggggg aaggggcgat
    10201 ccgtgggtgc tgcgctcaga caagatcggg cgtcccgact tcccccaatc ccgcgagccg
    10261 gatcgccacc ccaccgccta agctccctct tgcccctagt attgggggaa agagggtcga
    10321 ggaggtaggg ggcttgcggc ggctccacgt cgccgactgc ctattgccga ccgcccgtct
    10381 acggcgtact cgccgttggc acacggttcg caacctttgc caaatggccg gcacggctga
    10441 gggcgtcgag gtaggccttg gcgctggcga cgatgatatc ggtgtcggcg ccgcggcccg
    10501 tggccacgtg ttcgcccgac tttaccctca acacgacttc gccctgcgcg tcagtacctt
    10561 cggtcaccga cttcacgtgg tagtcgagca gctctgcttc catcccgacg atgcgatcga
    10621 cggccttgaa ggcggcatcg accggaccgt cgccgatggc agcatctgtg gcctcatcgc
    10681 cgttggggag gcggattcgc accgtcccgg tggcggtcat gttgctgccc gactgcacct
    10741 gtacccagat aaggtggtac acctcgctgg caacgcgttt ttcgtccatc agcagcgctt
    10801 ccaggtcgcg gtcgtccacc tctaccttct tgtcggcgag ctctttgaac gcgaggaagg
    10861 cgcgggacag ctcctcgttg tttagctggt agcccatatc ttccagccgt tgctgcagcg
    10921 cgtggcggcc ggaaagcttg cccagcacga gctgggtgcc ttcgggaacc ccgatcgact
    10981 tcggatccat gatctcgtag gtcgcccgca gcttgaggat gccgtcctgg tggatccccg
    11041 actggtggcg gaaggagttc ttgcccacga tcgccttgtt gtgttgcacc ggcatcccgg
    11101 tcaggtgctc gacgagcttg cttgtccggt aaagctgagt ggtgtcgatg cccatgccca
    11161 cgtcgaagag atcttcgcgc gtcttgatcg acatcacgac ctcctcgagc gcggcgttgc
    11221 cggcgcgttc gccgatgccg ttgacgcaga cctcgatctg gcgggcgccc gcgcggagcg
    11281 ccgcgagcga gtttgcagtc gaaaggccaa ggtcgttgtg gcaatgcacc gacacggtcg
    11341 ctttgtcgat gttgcggacg ttctgaaaaa tgccgcgaat gaactgctcg aactcctcgg
    11401 gaattgcgta cccgaccgtg tccggaatat tcaccgtcgt cgcaccggcg tcgattaccg
    11461 cctcgagcat cttccagacg tactcgggac gcgatcgggt cgcgtccatg gggctgaact
    11521 cgatgtcgct ggtgtagcct ttcgcccgtg cgaccatctc cgcagcctgg ttcagcacct
    11581 gctcgtagtc cttttgaagc tggtgcatga tgtggatgtc cgacgacgat aggaaaacgt
    11641 gaatgcgcgg cttttcggtc gcgttgcgaa gggcttccca cgcggcgtcg acgtcaccgg
    11701 gcaccgccct tgcgagcgat gcgaccgtgg cgccctttat ctctcgcgca atcgccatca
    11761 cagcatcgaa gtcgccgggc gacgaagccg caaacccggc ctcgattacg tccacgccta
    11821 accgctcgag ctgcttggcg atctgcattt tgtcgtcgcc gttcagcgct actccggcgg
    11881 cctgttcccc gtcacgcagg gtggtgtcaa agataattac acggtctggc attggctctc
    11941 tccgttcgcg gtctgcattc aattcccgac cggccctctc aagcccgatg gctggagggc
    12001 catataagaa cgagccgatg ccccgcgatc atcacttcac gtctcccgag ccgggctctc
    12061 ggcattgtct ccgagcatcc ccgcacgact gccgcgttga aagtctaccc caacgcctaa
    12121 cggggggtac tggtacccca acgcctaacg gggggtactg gtaccccaac gcctaacggg
    12181 gggtactggt ccgacggttg cgctccgcta tggcaactac ctactgccgc cccgtcactc
    12241 caaaatcgca atatccacgg gagggcggag ctctgagcac ttcgacagca cctggtggat
    12301 ctcgttgttc aggaactcgg ttcgcagccg ctcgtttacc ggttcgtcgc ggtcgatggc
    12361 cgtcagcgcc tgttgcgtaa tgatgtaatc gaagcgaggc gcccacttgc gtgcgccgag
    12421 gcgcgccgtg gtcgaacagt tgtcgaccat gtacgcgaca cccttaaagt gcatgtacgg
    12481 attgagcgaa tcaaccgcct cgatgatcga ctcgttggcg atctccgaat acgggtggcc
    12541 cttctctcta aggaggtcca cctgcgccat catcgtggcg acgtatacgc cggctgtcag
    12601 cggatgaata ggggtcttga gcgtttgtcg cctggcgcgc gccccctcgc caacctgcca
    12661 catctcggta ccgtcgatct tgcccatcgg gaaccgctca cttcgctcct gggccatgac
    12721 gacgctgcgg atctcgttgc cggaagaaac ctcgtcgtag atctccagca gcacgtcgaa
    12781 cgccgccgga tatgcggctg agtacgcctt gaggaaggtc tccttgtcgg cgtcgccgag
    12841 catgttgaac acgcccagga ttccatatct cgagatgccc ttgctgatcg gcccagtgac
    12901 agactcgacc gaattgatga aagcctcttc ctgggacgcc ccatgctgga cgaaccaacg
    12961 gtacaagcac tcgacgatgc catggacggc gccgagcagg atgccccgtt cgccaaagat
    13021 gtccgacttg tactccgact cgagcgtcgt ttggaacgcg aacggcgaac cgagacctac
    13081 cgcccacccg agtgcatagt cggttgcacg accggtcacg tcctgttgga tggcgaagct
    13141 ggagttgatg ccggcgccat cgacgtcagc gccctgctcg tacagccgcc gtaccgacgg
    13201 gcccatgccc ttggggcaga cgccgatgat gttgacgttt tcgggaaacg aagcgccgac
    13261 gttttttagg tgcccgagca ggaaaccgtg tgagagcccg agcgtagagc ccggtctcag
    13321 cgccgcgaag atccgctggt attcctcggc ctgcgcggcg tcggagatta gcagcagcac
    13381 caggtctgat tcggcgatga cctgatacat ctctcccaac gtgccgctcg cccgtgtaaa
    13441 gcccgcccgc tcggcggccg gaattgaggc ggagccttcg cgcaggccga tcttcaccgt
    13501 gatggccgtg ccggcgaggg aatcccgcag gttttgcgcc tgcgcgggcg cctgcgacga
    13561 ccagcccaga acacctatct ggcgaatgcc ctcaaaggcg tcggcgagcc gcggaaacag
    13621 gctccgtcca ccacggacaa tgaattcctc ggtgctgccc agccgcagtg gttccttttc
    13681 aaaggtctcg gtggtaaagg ttgccggcat agtctcgggg actcctcggg ccactcgcgg
    13741 gccatcttgg aaatttgagg ttcagtctga aacgaacggg agaggcttgt tcggcatgcg
    13801 cccttcgcgg cggtggaact ggcggatttc ggccggctcc acgtctaccg tggtagaagt
    13861 cgcgccgcgc gttagtgcca cgcgtccggt acgcacgatc tcgcggatgc cgtagggtcg
    13921 cagcacttgc agcaggctct ccagcgtctc ttcgtcgccg accgcctgca cgaccagcgt
    13981 cgtatcggtg acgtcgataa tctcagctct gaaa
//
LOCUS       DATCPE010000047         5511 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U7219
            k141_7618, whole genome shotgun sequence.
ACCESSION   DATCPE010000047 DATCPE010000000
VERSION     DATCPE010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955033
            Sequence Read Archive: SRR1586279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 5511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 16:49:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 640
            CDSs (total)                      :: 631
            Genes (coding)                    :: 627
            CDSs (with protein)               :: 627
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5511
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7618"
                     /isolate="SMAG_U7219"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(13..2646)
                     /gene="alaS"
                     /locus_tag="VIM45_01495"
     CDS             complement(13..2646)
                     /gene="alaS"
                     /locus_tag="VIM45_01495"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEY8819992.1"
                     /translation="MNSDQVRERFLTFFIERGHKQIPSATLVPWGDPTLLFTSAGMVQ
                     FKPFFTGKDSPPALRMTSVQRCFRTSDIESVGDVSHHTFFEMLGNFSVGDYFKDQAIS
                     WAWEFVTKELGIPAERLWNTVYTDDDQAFDLWVGQGQKPEKIFRYGEAEGNFWTSGDL
                     GPCGPCSEIFYDFGEAIGCGPDCEPAHENCYRFLEIWNLVFMSMYQDENGERTPLPKA
                     NIDTGAGLERITRVLEDSASSYETDLFDPILRAISDAIGVEYGKDEESRRMMRVIADH
                     ARAVTFLVADGVLPTNEGRGYVVRRLIRRSVYYGRVLAAEGPFLAKVADAVIDRMKRY
                     YPFLDEQRTNVHSALTGEERRFGDTLEAGVTRLDGLIASLRGQGDALPGDEIFRLYST
                     YGVPLEVIEEIAEQHGLNIDREGFESELERERERGRAGGEFLAEATSQARLELAGTGR
                     GGRFIGYESLQVDTAITGIFAGSEVLDRANAGRQIQVVLDATPFYPEGGGQVGDAGEI
                     RTESGVARVTDTQRDEGLILHEATVVDGTIAVQQLVAATVDPIRRRNTTRNHTGTHLL
                     HAALREVLGRHVRQMGSLVAPDRLRFDYQQPQAPTNQQRLEVEHLVNEKIREDIPVAT
                     KVMNKEAALGEGVLAFFGDKYGDEVRVVEMVDSGHRFSAELCGGTHVDETGELGLLLL
                     VNDASIGAGVRRVEALTGSSAERYIEEQLARIDRLSRTLGVPPTGLEARVEGLVAELE
                     AVRRQQALMQREAGRQNADSLVASAERIGGAALIIARVNAPNVESLRQIGDVVRQKVG
                     SSVVVLTGASGKKPPAMVMVSSDLSQKVKAADVLNKGLVAAEARGGGRPELAQGGINN
                     PAALDRAFEDARRLVKELLSD"
     gene            2828..4099
                     /locus_tag="VIM45_01500"
     CDS             2828..4099
                     /locus_tag="VIM45_01500"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEY8819993.1"
                     /translation="MDVDSWLGVLLLLGCALGLFLTAVSSAAGIFNRRPYRPPVGRGA
                     ARPENLHQFLHDRESFISSLVFARTVSIVTGGITAGVIVMRRTGVSARPAIVTLVLVM
                     LAAELIEGIPRVVVSHNPERWHDRVVLITVAIHGAFFVPAMLIDLPGKALLRFARFRP
                     NADDDDQDEEIVRLVELEESQGRIEDDERQMIRAVIELEDTTAREIMVPRIDITAVGT
                     GESMQDVAAIIAVHGYSRIPLYEGNIDNIVGVVYAKDVLARFAQGAGDVDIRTIARPP
                     LFVPETKRVDELLSELRDRRIHMAVVVDEYGGTSGLLTIEDLVEEIVGEIEDEYDHAE
                     PNVVLTGEHDAVVDARQRVDILKELFDVEPDQGDFDTVGGFIIHQLGKIPSPGDTVAI
                     NGLSLKVLSVTGRRIRKVRITRMQSDTAGQN"
     gene            4191..5105
                     /gene="argF"
                     /locus_tag="VIM45_01505"
     CDS             4191..5105
                     /gene="argF"
                     /locus_tag="VIM45_01505"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEY8819994.1"
                     /translation="MRGRHLLTVGDLDARELSLILDTSARFKAAPPPPLLAGKSVALL
                     FEKPSLRTRVSFDVGVYRLGGHLTYLSPPEVGLGVREAVKDVARVLSGYVDLIAARTM
                     TQSTVEELASYASVPVINALSDTEHPCQALADLLTIQENCGRLAGVRIAYIGDGNNVA
                     ASLVQSCALMGMDITLASPEGYDVPETALARARGYAEKSGGSITIVREPEEAVTDVQV
                     IYTDVWVSMGQEEETASRREAFAPYQVDMALLRRAPAEVIVMHDLPAHRGEEITDEVM
                     ESSHSRVFQQAENRMYTEQAAMALILGD"
     gene            5110..>5511
                     /locus_tag="VIM45_01510"
     CDS             5110..>5511
                     /locus_tag="VIM45_01510"
                     /inference="COORDINATES: protein motif:HMM:NF039908.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8819995.1"
                     /translation="MPQPPESVKTFIDTLLRIDPVSAIDILLIAAIIYAVLVWLKGST
                     GMSLVRGAAFVILVGLVAGSLLNLTVVNWLLRNSVPALLVAVPIVFQPEIRRALERLG
                     RTRVASRRSRRASDVVLGALVQASAELAMRRL"
BASE COUNT         1002 a         1693 c         1687 g         1129 t
ORIGIN      
        1 tcgcccgtag catcagtcag agagcaactc cttgactaac cgcctggcat cctcgaatgc
       61 ccggtccagc gcggccgggt tgttgattcc gccttgggca agttcaggtc gcccgccgcc
      121 cctggcctcg gccgctacca atcctttgtt tagcacatcc gcggccttca ccttctggct
      181 caaatcagaa gagaccataa ccatagcggg cggcttctta cccgaagctc ccgtgagtac
      241 gacgacggag ctgcccacct tctgccggac aacgtcgccg atctgacgca gggattctac
      301 gttcggagcg ttaacgcggg cgataataag cgctgcgccc ccgatgcgtt cggctgaggc
      361 gaccagtgag tcggcatttt gccgtcccgc ttcgcgttgc attagtgctt gttggcgacg
      421 taccgcttcg agctcggcga cgaggccctc cacgcgggcc tcgagcccgg ttggggggac
      481 gccgagcgtc cgagagaggc gatcgatcct cgctagctgc tcttcgatat aacgctcggc
      541 cgaggagccg gtcagtgcct ccacacggcg cacgcccgcg ccaatacttg cgtcgttcac
      601 cagcaacagc aggccgagct caccagtctc gtctacgtgc gtgccgccgc atagctcggc
      661 actgaagcgg tgtccactgt ccaccatctc cacgacacgt acttcgtcac cgtatttgtc
      721 tccgaagaat gccagcacgc cctcgccgag cgctgcctct ttgttcatga cctttgtcgc
      781 gacgggtatg tcctcgcgga tcttctcgtt gaccaggtgc tcgacctcga gccgctgttg
      841 gtttgtcggc gcctggggtt gctggtaatc aaagcgcagc ctatccggag cgacaagcga
      901 gcccatctgc cgtacatgcc ggcccaagac ctcgcggagt gccgcgtgca ggaggtgtgt
      961 gccggtgtga ttccgcgtcg tgttccggcg gcggatcggg tcaacagtcg ccgccaccag
     1021 ctgctggacg gcgatcgtgc catctacgac ggtagcttcg tgcaggatca aaccttcatc
     1081 acgctgcgtg tccgtcaccc gggctacacc agattcggtg cgaatctcgc ctgcatcgcc
     1141 gacctgtcca ccgccctcgg gataaaacgg tgttgcgtcg agcacgacct ggatctgccg
     1201 gccagcgttc gcacggtcga gaacctctga accggcaaag atcccggtga ttgccgtatc
     1261 gacttgcagc gactcgtaac cgatgaacct gccgccgcgg cccgttcctg cgagctcgag
     1321 ccgggcctgg ctcgtcgctt ctgccagaaa ttcgccgccg gctcgaccgc gctcccgctc
     1381 gcgttccaac tcgctctcga aaccttcgcg atcgatgttc aaaccgtgct gttcggcgat
     1441 ctcctcaatt acctccaatg gcacgccgta ggtgctgtag agacggaaga tctcgtcgcc
     1501 aggaagcgcg tcgccctgac cacgaaggct cgcgatcagc ccgtcaagcc tcgttacacc
     1561 ggcttcgagc gtgtctccga accgccgttc ctcaccggtg agtgcagagt gaacgttggt
     1621 tcgctgttcg tcgaggaacg ggtaataccg tttcatacga tcgatgacgg cgtccgcgac
     1681 cttggccagg aacgggcctt ccgctgccag tacgcgcccg tagtagacgc tgcggcgaat
     1741 gagccgtcgt acgacgtacc cgcgcccctc gttggttggc aatacgccat cggcgacgag
     1801 aaaggtgacc gcacgcgcgt gatcggcaat gacccgcatc atgcgccttg actcttcgtc
     1861 cttgccgtat tccaccccga tcgcgtccga gatcgccctg aggatcgggt cgaagaggtc
     1921 agtctcgtag cttgacgcgc tgtcctccag cactcgcgtt atccgctcca ggccagcgcc
     1981 ggtgtcaatg ttggcttttg gcaacggcgt gcgctcaccg ttctcgtcct ggtacatcga
     2041 catgaagacc aggttccaga tttcgagaaa gcggtagcag ttctcgtggg ccggctcgca
     2101 atcgggtccg cagccgattg cttcgccgaa gtcatagaag atctcggaac atggtccgca
     2161 agggccaagg tcgcccgaag tccagaaatt gccctctgcc tcgccgtaac ggaagatctt
     2221 ttccggcttc tggccctggc ctacccaaag gtcgaacgcc tgatcgtcgt ccgtatagac
     2281 ggtgttccat aatcgctccg ctgggatgcc gagctctttg gtcacgaact cccacgccca
     2341 cgagatcgcc tggtccttga agtaatctcc cacggaaaag ttgccgagca tctcgaagaa
     2401 cgtgtggtgg ctgacgtcgc ccacactttc gatgtcgctt gtgcggaagc aacgctggac
     2461 cgacgtcata cgcaaggcag ggggcgaatc cttgccggtg aagaagggct tgaactgcac
     2521 catgccggcg ctcgtgaaaa gcaacgtggg gtcgccccac ggcaccagcg tcgcggacgg
     2581 tatctgtttg tggccgcgtt caatgaagaa ggtgaggaag cgttcccgca cctgatcgct
     2641 gttcacgagt gacctcgacc gccgttccct aagtagttcc gtgcgcgatg tcgaccgggc
     2701 cgccaggccc gggattgggc ccgattatac catggggccc atcgggtcac tgagatggag
     2761 atataatgat cgaaatcatc aaccggttgg cattttcacc cggccgcgga aggagccgtt
     2821 cgtccgcttg gacgtagata gttggttggg cgttttgctc ttgctgggct gcgctctcgg
     2881 gctcttcctc accgccgtct cctcggcggc cggaatcttc aaccgaagac cgtatcgccc
     2941 gcccgttggc cgcggggccg cgcgtcccga aaatcttcat caattccttc acgaccgcga
     3001 gagtttcata tccagcctgg tattcgcccg gacggtcagc atcgtcacgg gcgggatcac
     3061 cgccggagtt attgtcatgc ggcgcaccgg cgtgtcagcg cgacctgcga tcgtaacgct
     3121 tgtgctggtg atgcttgcgg ccgaattgat cgaaggcata ccgcgggtcg tcgtctcgca
     3181 caaccccgaa cgctggcatg acagagtcgt cctcattacc gtggcaatcc acggcgcttt
     3241 ctttgtcccc gcgatgctta tcgatttgcc cggcaaggcg ttattgcgct tcgcgcgctt
     3301 tcgtccgaat gcagacgacg acgaccagga tgaagagatc gttcgcctgg ttgagcttga
     3361 agagagccag ggccgcatcg aggacgacga gcggcagatg attcgggccg taattgaatt
     3421 ggaggacacg acagcgcggg agatcatggt tccgcgaatc gacatcaccg cggtggggac
     3481 cggcgagtcg atgcaggatg ttgcagccat aattgccgtt cacggctaca gccgcattcc
     3541 gctgtacgag ggcaacatag ataatattgt gggtgttgtt tacgcgaagg acgttcttgc
     3601 ccgcttcgcg cagggcgcag gggacgtcga tattcggacg atcgctcgcc cgccgctgtt
     3661 cgtgccggaa accaagcgcg tcgacgagtt attgagtgag ctgcgcgatc ggcgcataca
     3721 catggcggtc gtggtggacg aatatggcgg cacttcggga ctgttgacga tcgaagacct
     3781 ggtcgaagag atcgtcggtg agatcgaaga tgagtacgat cacgcggagc caaacgttgt
     3841 gctgaccggc gagcatgacg ccgtcgttga tgcgcgacag cgcgtggaca ttttgaaaga
     3901 actattcgac gtcgagccgg atcagggcga tttcgacacc gtcggcggct tcatcattca
     3961 ccaactcggc aagattccgt cgccgggcga caccgtggct ataaacggcc tgagcctcaa
     4021 agttctatct gtaaccggca ggcgcatccg caaggtgcgc atcaccagga tgcaatcgga
     4081 cactgctggt cagaactgat gccggccggg ggagtaacga gaacgtaacg aaaccccagg
     4141 cggcagcccc tatgctatac ttgccgcgct cacgagcgca ggagtaggga atgcgcggca
     4201 ggcacctttt gaccgtgggt gacctggacg cacgggaact gtcgctgatt ctcgatactt
     4261 cggcgcgttt caaagctgcc ccgccgccgc cgctcctcgc cggcaagtcc gtcgctctcc
     4321 tcttcgaaaa gccatcgctt cgcacacggg tcagcttcga cgttggcgta tatcgccttg
     4381 gcggtcacct cacctacctg tcgccgccgg aggtgggtct tggcgtgcgt gaggctgtga
     4441 aggacgtggc gcgggtactc agcggctacg tagacctgat tgcagcgcgg acgatgacgc
     4501 agagtacggt cgaagaactc gcctcatacg ccagcgttcc ggtcattaac gctctttcag
     4561 atacagagca cccatgccag gcgttggccg acctgttgac catccaggag aactgtggcc
     4621 ggttggccgg tgttcggatc gcgtatatcg gggacggtaa taacgttgcc gcgtcgctcg
     4681 ttcagtcgtg cgccctgatg ggcatggaca ttacgctggc ctctcctgag ggctatgatg
     4741 tgcccgaaac ggcgctcgcg cgcgcgcgcg gttacgctga gaagtcgggt ggttcgatta
     4801 cgatcgttcg agagcccgag gaagccgtaa cggacgttca ggtcatctat acggacgttt
     4861 gggtgagcat ggggcaggaa gaggagactg catcgagacg cgaagccttt gcgccctacc
     4921 aggttgatat ggcgctgctt cgtcgggcgc cagcggaagt aatcgtgatg cacgacctcc
     4981 cggcccaccg cggtgaagag ataaccgacg aagtgatgga gtcatcgcac tcgcgcgtgt
     5041 tccagcaggc cgaaaaccgc atgtataccg aacaggccgc gatggcgctc attttaggcg
     5101 actgatccca tgccccagcc ccccgagtcg gtaaagacct tcattgacac gctactgcgt
     5161 attgatccgg tcagcgctat cgatatcctc ctgattgcgg cgatcattta cgcggttttg
     5221 gtctggctga agggttcaac cgggatgtcg ctggtgcgag gggcggcgtt cgtcatcctg
     5281 gttgggcttg tcgccggcag cctgctcaac ctcacagtcg tcaactggct tctgcggaat
     5341 tcggtaccgg cgctgctggt ggccgtgccg atcgtctttc agccggagat ccggcgggcg
     5401 ctggaacgcc tggggcgcac gcgcgtggcg tctcggcgca gccggcgcgc ctcggatgtg
     5461 gttctcggcg cccttgtgca ggcgagtgcg gaacttgcaa tgcggcggtt g
//
LOCUS       DATCPE010000048         2059 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U7219
            k141_364788, whole genome shotgun sequence.
ACCESSION   DATCPE010000048 DATCPE010000000
VERSION     DATCPE010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955033
            Sequence Read Archive: SRR1586279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 16:49:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 640
            CDSs (total)                      :: 631
            Genes (coding)                    :: 627
            CDSs (with protein)               :: 627
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_364788"
                     /isolate="SMAG_U7219"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..354)
                     /locus_tag="VIM45_01515"
     CDS             complement(<1..354)
                     /locus_tag="VIM45_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015130651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8819996.1"
                     /translation="MCDFDAAAIPDVESMITDEGRAALSELHDTVTAAIAEGGDPEAI
                     IGRLRGHFAAFLKRDGWLPEPCRQAIPGKAASYALLRSKDPEFVLFSMVLPAGESTKV
                     HNHLAWGLIGLWQGRQ"
     gene            complement(859..1809)
                     /locus_tag="VIM45_01520"
     CDS             complement(859..1809)
                     /locus_tag="VIM45_01520"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY8819997.1"
                     /translation="MSVRIGLALGGWPFGANDCAPFWDFVDQAEALGYDSLWLSDRLI
                     STSPIVEPVTGLAAIAGRTRKLKFGMSVLVLPLRNPVIVAKEIATIDFLSGGRMLPAF
                     GIGTEDPREYEAAGVAKEERGGRTDEAVAILRRLWSEEHVTHRGRYYRMTDVTIAPRP
                     AQRILPIWFGGRSEAAYRRIGRSGDGWLASFLTTEEFADGAERIRANAAAAGRDIDED
                     HYGVILSVTIATSVEQARQLAAPALRRFRPDVDIERLSIFGTPDDCSARIDEYLRAGA
                     SKFVLRPACPPDQLSAQLDIIARDIAPRFDRTMATVGATS"
     gene            complement(1865..>2059)
                     /locus_tag="VIM45_01525"
     CDS             complement(1865..>2059)
                     /locus_tag="VIM45_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8819998.1"
                     /translation="EGARRAWERIRDVSHLNWPDNRTWSLSGGAAGYNVDVGSVENML
                     SDADRRLALEKRRLRAEHES"
BASE COUNT          390 a          658 c          655 g          356 t
ORIGIN      
        1 ctggcgaccc tgccacaggc cgatcagtcc ccaggcgagg tggttgtgaa cctttgtgct
       61 ctcgccggcc gggagcacca tgctgaacaa cacgaactcc gggtcctttg aacggagcaa
      121 cgcgtagctg gcggccttgc ccggaatcgc ctgccggcac ggctcgggca accatccatc
      181 gcgcttgaga aacgcggcga agtgcccgcg tagccgtccg atgatcgcct cgggatcgcc
      241 gccttcggca atggcagcag tcacggtgtc atgcagttcg ctcagcgctg ctctgccttc
      301 gtcggtgatc atcgattcga catccggaat tgcagcggcg tcaaagtcgc acaccggcaa
      361 acctcctgcg gtcgcaatcg cattgcgtcg tcatggctat tataggttcg cgccgccgca
      421 cggtactaac gcgggaaagt gctggcgggc tcaacccgca ggccctgagg tgaactggcc
      481 gacatatgcc ggctgcacgg ggctgccaca gaccgtgcat ggagcgtctg agggagaacc
      541 cttgcagtct ttgcactcaa cccagttaca caacggacac acgatggcgg catagacggg
      601 cggcagcatg ctggttgcag gaggcatgcg gcaggcgagg ggccgagcac agccactgca
      661 aataggcgta attctggtac cggcggcccg gccgtcctgc gcaccaccca cgggcctgga
      721 ggcgccggct cccggtcccg agtacggcac ggaaggcgac ggcaaggcgt cagccggttt
      781 gtgccgccgt tttctgaaaa gccacatccc tgctccgatc agtgacgccg tacggagcct
      841 gacaacgcca atatcgcgtc aggaggttgc tccaacggtg gccatcgttc ggtcaaacct
      901 gggagcgata tcccgcgcaa tgatgtcgag ctgtgccgaa agctgatccg gagggcaggc
      961 gggacggagg acgaacttgc tggcgccggc gcgcaggtat tcgtcaatgc gggcggagca
     1021 gtcgtccgga gtgccaaata tactgagtcg ctcgatatcc acgtcgggac gaaaacgtcg
     1081 caaggcgggt gccgcaagct ggcgcgcctg ctcgacgctc gttgcgatgg tcaccgaaag
     1141 gatgacgccg tagtggtcct cgtcgatgtc gcgtccggcg gcagcggcgt tggcccgtat
     1201 tcgttcggca ccgtcggcga actcttcggt ggtaagaaac gacgccagcc aaccgtcacc
     1261 tgaccgcccg atacgccggt aggcggcttc gctgcgccct ccaaaccaga tcggcagtat
     1321 ccgttgggcg gggcgcggcg cgatcgtcac gtctgtcatc cggtagtagc ggccgcggtg
     1381 cgtcacatgt tcttccgacc agagccgccg cagaatcgcg accgcctcgt cagtgcgtcc
     1441 gccacgctct tccttcgcaa caccggcggc ctcatattcg cgcgggtctt cggtgccgat
     1501 gccaaacgcg ggtagcatac ggccgccgga gaggaaatca atcgtggcta tctccttggc
     1561 cactatgaca ggattgcgta gcggcaggac gagcacgctc attccgaact tgagtttgcg
     1621 cgtgcgtccg gcaatagcgg ccagcccggt aaccggctcc acgatgggcg acgtggagat
     1681 aagccggtcg cttagccaca gcgaatcgta gccaagcgct tcagcttgat cgacaaaatc
     1741 ccaaaacggg gcgcagtcgt tggcgccgaa tggccagccg ccgagcgcga gcccgatgcg
     1801 tacactcacc gtcgtattat ccccgattga aaggagttga acgccggggc ccgtaaagtc
     1861 gaatctacga ttcgtgctcg gcccgaaggc gtcgcttttc gagcgccagc cggcgatcag
     1921 cgtcggagag catgttctcg accgagccga cgtcaacgtt atacccagcc gctccgccgg
     1981 acaacgacca ggtgcggtta tcgggccagt tcaagtgcga cacatcgcga attcgctccc
     2041 atgcgcggcg cgcgccctc
//
LOCUS       DATCPE010000049         4752 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U7219
            k141_105496, whole genome shotgun sequence.
ACCESSION   DATCPE010000049 DATCPE010000000
VERSION     DATCPE010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955033
            Sequence Read Archive: SRR1586279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 4752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 16:49:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 640
            CDSs (total)                      :: 631
            Genes (coding)                    :: 627
            CDSs (with protein)               :: 627
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4752
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105496"
                     /isolate="SMAG_U7219"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            290..982
                     /locus_tag="VIM45_01530"
     CDS             290..982
                     /locus_tag="VIM45_01530"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HEY8819999.1"
                     /translation="MSDERKTALVVGAHPDDPDFGAGGTAALWSRDGWEFYYLVCTNG
                     SKGSADPDMDPVKLIEFRRTEQRAAAQALGVVDCFFLDGVDGELQPTREFLGHVVRYI
                     RTLKPDAVFTHSTEIVIRNSFINHTDHRCTGQVTLDAIYPAARDILNFPEQIADGLEP
                     HKVLDIYIWGSNEPNFTVEIGEVADVKVDALTHHMTQFAHREGFRESVKERWKNEEGR
                     LVETFRRVQMRG"
     gene            994..1497
                     /locus_tag="VIM45_01535"
     CDS             994..1497
                     /locus_tag="VIM45_01535"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEY8820000.1"
                     /translation="MTFCIECGNRLQERVAFGRPRMVCPACEHVHFEDPKVAVGVVAT
                     RGGRILLTRRNHEPKMGEWSFPAGFVDAGEDVIEAAAREALEETGVQVQVGPLLGVFQ
                     ERASRVVFLAYAATAPEGELACGDECMEVRFFEPDALPALAFENDTAILAAWRRTVAG
                     AEAPYSN"
     gene            1497..2696
                     /gene="queG"
                     /locus_tag="VIM45_01540"
     CDS             1497..2696
                     /gene="queG"
                     /locus_tag="VIM45_01540"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEY8820001.1"
                     /translation="MLSAEALKRRVLDMGFVAVGIAGAETDSAARFVFHERIDLGMYD
                     GLPWFTGERADRATEPSRSLAGAASVITLAAPYKTEYEPAESLDGLRGRVARYAWGRD
                     YHRVLEHRLKQLVTWLDEEYGARSRGLVDYGPLAERAYAARAGIGWFGKNTNLLLPGI
                     GSWVLLAELITTVAFEPDEPLKKSCGSCTRCISACPTGAISGPYVVDNRRCISFQTIE
                     NRGWIPRDLRPLMGDWVFGCDLCQDVCPVGAGGERTMPEFRPASADAALPELTALLAL
                     TEEQFLERFRGRAIMRAKRAGLARNACIALGNLGDPRAVPALGVAVRWDPSAIVRGHA
                     AWALGRIGGAVARSLLEEALQAERDAVARAEIEAALEGEEQPPDGAGIVRDPIAWLAG
                     PWRSSRR"
     gene            2804..3793
                     /locus_tag="VIM45_01545"
     CDS             2804..3793
                     /locus_tag="VIM45_01545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY8820002.1"
                     /translation="MIRLIRRFALPLLLMVVTLILPVGVHAGTVASYPVQRSLPQQSA
                     GTPLRQVDWASVLTSDPNLQTDDECVPFPLLALDKPICITVAVTPPVAVNSLSGVVDS
                     FSGYAVIGDGVMYGDIDGDGVDEAVIETQSGGTGGSFGFLVYHQASPRPRLVAAVPGY
                     KLGVRIDSGTIVARQPFYFGFEGNCCPTGLTEIGYRLIGGTLTATYGPNYSLLSLDVE
                     RPATPAEITVAAFYRALSTRDFDAAYSLLSPAYQATHPFSSWKAGYANTQDIEADVDL
                     GNAPGEVTVAITASDRTPAGGRTVSSFAGSWFTVPDANAPLGVLLDHAVIGVV"
     gene            3839..4414
                     /locus_tag="VIM45_01550"
     CDS             3839..4414
                     /locus_tag="VIM45_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HEY8820003.1"
                     /translation="MTDSEAGTSGDAVIRQAAGKLTLIERRAIEAGVLVPLIRAFQNE
                     FGEDRANAVARQTIIDLAEEQGRKNADHKGRNDLLAFTENMQAFSGGGALEVEMVERD
                     AARVGFNVRRCRFAEMYRDMGAGDIGAVLSCNRDFSNVNGFNPEIKLTRTQTIMQGAE
                     YCNFRYSYAAPDPGASSGAVGSRGPNPRPLP"
BASE COUNT          853 a         1442 c         1559 g          898 t
ORIGIN      
        1 gtcttgccgc gtagatcgca gtagcggcgg tagggctgat tcttagtgta ttggaaggtg
       61 aagacgtcca gcgcaagttc gttgaactgc gtctcggcca tcggcccatc ggtcccggac
      121 cggacgaccg acaggatttg cgcttgcagt gtttccgggg tgggcggctt tcgtgtcaac
      181 tgcttcctct acggcaccag aatagcacgc ccgttccagc atcccggcgc caattgtggc
      241 tggttcgaga tggtccgtac aatcggggta gagcacggag gacgatcgca tgagcgatga
      301 acggaaaacg gcgctggtgg tcggcgccca cccggacgac cccgacttcg gcgccggtgg
      361 caccgcggcc ctctggtcgc gagatggctg ggaattctac tacctcgtct gcacgaacgg
      421 cagcaagggc agcgccgatc cggacatgga cccggtgaag ctgatcgaat tccgccgcac
      481 ggagcagcgc gcggctgcgc aagcgctcgg cgtggtcgat tgcttcttcc tcgacggagt
      541 ggacggtgag cttcagccga cgcgcgagtt cctgggacac gtcgtccgct atattcgcac
      601 cctcaaaccc gacgccgtct tcacacacag caccgagatc gttatccgca acagcttcat
      661 caatcacacc gatcaccgct gcaccggaca ggtgacgctc gacgccatct acccagcagc
      721 gcgggacatc ctcaatttcc ccgaacagat cgcagacggc ctggagccgc acaaggtgct
      781 ggacatctat atctggggct ccaacgagcc gaacttcacc gtggaaattg gcgaagtggc
      841 ggacgtcaaa gtggacgcgc tgacgcatca catgacgcag tttgcgcacc gcgaaggttt
      901 ccgtgagtcg gtgaaggaac gatggaagaa tgaggaaggc cgcctggtgg agaccttccg
      961 ccgagtgcag atgcggggct agtgcttgtg ccgttgacgt tctgtatcga gtgcggaaac
     1021 cgcttacaag agcgggtggc gtttggccgt ccgcgcatgg tctgtccggc gtgcgagcac
     1081 gttcacttcg aagacccgaa agtagccgtt ggcgtcgtcg cgacacgggg cgggcggatc
     1141 ctgctcacgc gccgcaatca cgagccaaag atgggcgaat ggagctttcc ggcgggcttc
     1201 gtcgatgccg gcgaagacgt catcgaggca gcggcgcgag aggcactaga agagactggc
     1261 gtccaggtcc aggttgggcc gctgctcggc gtctttcagg aacgcgccag ccgggtcgtc
     1321 ttcctggcgt atgccgctac tgcgcctgag ggcgagcttg cgtgtggaga cgagtgcatg
     1381 gaggttcgct tcttcgagcc ggacgccctg ccggcgcttg cgttcgaaaa cgacactgcc
     1441 atcctcgcgg cctggcggcg gacggtcgcc ggtgccgaag cgccgtactc gaactgatgc
     1501 tctccgcaga ggcgctcaaa cgcagggtgc tcgacatggg cttcgtagcg gttggcatcg
     1561 ccggagctga aaccgacagt gccgcgcgct tcgtcttcca cgaacggatc gacctcggca
     1621 tgtacgacgg gttgccctgg ttcaccggcg agcgcgccga tcgcgccacc gagccgtcgc
     1681 gcagtcttgc aggcgcggca tcggtcatca cgctggccgc tccatacaag acggagtacg
     1741 agcctgctga gtcgctagac ggcctgcgcg gccgcgtagc ccgctatgcg tgggggcgcg
     1801 attaccaccg cgtgctggaa caccggctca aacaactggt gacgtggtta gatgaagagt
     1861 acggtgcgcg gtcgcgggga ttggttgatt acggaccgct cgctgagcgc gcgtacgcgg
     1921 cccgcgcggg catcggctgg ttcggaaaga atacgaacct gttgctccct ggcattggct
     1981 cgtgggtgct gctggcggaa ttgatcacca ccgtcgcatt tgagccggac gagccgctga
     2041 aaaagagctg tggctcctgc acccgctgca tcagcgcctg cccgaccggc gccatcagcg
     2101 gaccgtacgt tgtggacaac cggcgctgca tctcatttca aacgatcgaa aaccgcggct
     2161 ggatcccgcg agacctgcgc ccgctgatgg gcgactgggt gttcggttgc gacctttgtc
     2221 aggatgtttg tcctgtcggc gccggcggcg agaggactat gccggagttc cggcccgcct
     2281 ctgccgacgc ggcactgccc gagctcaccg ccctgctcgc gttgaccgag gaacagttcc
     2341 tggaacggtt ccgtggtcga gccatcatgc gggcaaagag ggccgggttg gcacggaacg
     2401 cgtgtatcgc gctgggtaac ctcggcgatc cacgagcggt gcccgcgctt ggcgtggcag
     2461 tgcgttggga tccgtccgcc atcgtccgcg gtcatgccgc gtgggcactt ggacgcattg
     2521 gcggcgccgt ggcccgctct ttgctggaag aggcgctgca agccgagcgg gacgccgtcg
     2581 cccgcgccga aatcgaagcg gcgctcgagg gcgaagagca gccgccggat ggcgccggca
     2641 tagtccggga cccgatagcg tggttggccg gcccctggcg aagttctcgg cggtagtccc
     2701 ggagtctgtg atgctgcaca ggcgcaatcc agcacagcgc tgggtcacac ggcagcgccg
     2761 acgcgtacaa taaagctcaa gtctcggctc ggagccccgc cacatgattc gtttgatccg
     2821 ccgttttgct ctccccttat tgttgatggt cgtgacgctg attctgcctg ttggagttca
     2881 cgcgggaacc gttgcctcgt atcctgtgca gcgctcgctg ccgcaacaga gcgccggcac
     2941 gccccttcgt caagtcgact gggcgtccgt gctaacgtct gacccgaacc tgcaaactga
     3001 cgacgagtgc gttccgtttc cgttactggc gctcgacaaa ccgatctgca tcaccgtagc
     3061 cgtgacacct ccggtagctg tcaattcatt gagcggcgtc gtcgactcct tctcgggcta
     3121 cgcggtgatc ggcgacgggg ttatgtacgg cgatattgat ggggacggtg tcgacgaggc
     3181 ggttatcgag acccagtcag gagggaccgg tgggagtttc ggctttctcg tgtaccacca
     3241 ggcttcaccc cgaccgaggc ttgtcgccgc cgttccgggg tacaaattgg gagttcggat
     3301 agatagtggg acgatcgtgg ctcggcagcc gttctatttc ggcttcgaag gcaactgctg
     3361 cccgaccgga ctcaccgaga taggctaccg tctcatcggc ggcacgctca ccgcgaccta
     3421 tggaccgaac tattcgctgc tgtcgctgga tgtcgagcgc ccggccacac cggccgaaat
     3481 caccgtcgcg gccttctatc gtgccctatc tacgcgcgac ttcgatgctg catattcgct
     3541 gctaagcccg gcttatcaag cgacgcatcc ctttagctcg tggaaagccg gctacgcgaa
     3601 tacccaggac atcgaagccg acgtcgatct cggcaacgcg ccgggcgagg tcacggtcgc
     3661 gatcacggca tcggaccgga cgccggcagg aggtcggacg gtttcttcgt ttgccggatc
     3721 atggtttacc gtgccagatg caaatgctcc gctcggagtg ctgctcgacc atgccgtgat
     3781 cggcgtggtt tgatcaaatg acgtgggcgc attcgagatt tgtgcgggag atacgtcgat
     3841 gacagattcg gaagcgggca caagcggcga tgctgtcatc cggcaagcgg cagggaagct
     3901 cacactgatc gagcgccggg cgatagaagc cggcgtgttg gtgccgctga tccgtgcgtt
     3961 ccaaaatgaa ttcggagaag atcgcgccaa cgccgtcgct cggcagacga tcatcgacct
     4021 cgcggaagag caaggccgca agaacgccga tcacaagggc cgcaacgacc tactggcgtt
     4081 cacagaaaac atgcaggcgt tcagcggcgg cggtgcgctc gaagtcgaga tggtcgagcg
     4141 cgatgccgcc cgtgtgggtt tcaacgtgcg gcgttgccgg tttgcggaga tgtaccgcga
     4201 catgggggcg ggcgatatcg gtgctgtcct ctcctgcaac cgcgacttct ccaacgttaa
     4261 cggcttcaat cccgagatca agctcacgcg cacccaaacc atcatgcagg gcgcggagta
     4321 ttgcaacttc cgttacagct acgcggcacc tgaccccggt gcctcgtcgg gggcagtggg
     4381 cagtcgtgga cctaaccccc ggccccttcc ctaaccaggg aaggggagct tgtagtcggt
     4441 agttggtgaa gtagtccgtc cacgtatagg ggcgcatcgc gatgtgccta atccgacgct
     4501 gcagtcatgc ccgtacacgg cacccctaga tcgccccttc ccggcgttag ggaaggggta
     4561 tgggggttag gtcgccgtgt gctgcaaccg agcccgatcg gtgcagccaa cgatcgcccc
     4621 ttcccggcgt tagggaaggg gaagcaaccg tcttaatggt aggtgttcga acgatggttg
     4681 gtcttgggca tacctcctcg ttgttgctgc cgttgtgcta cgctagcgat gcactaacgg
     4741 gaacgggacg gt
//
LOCUS       DATCPE010000050         3795 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U7219
            k141_365004, whole genome shotgun sequence.
ACCESSION   DATCPE010000050 DATCPE010000000
VERSION     DATCPE010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955033
            Sequence Read Archive: SRR1586279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 16:49:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 640
            CDSs (total)                      :: 631
            Genes (coding)                    :: 627
            CDSs (with protein)               :: 627
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3795
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_365004"
                     /isolate="SMAG_U7219"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            21..263
                     /locus_tag="VIM45_01555"
     CDS             21..263
                     /locus_tag="VIM45_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005992879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEY8820004.1"
                     /translation="MYLLRCRDGTLYTGISTDVVRRVQEHDTGRGARYTRGRSPVQLL
                     AAWPYADESAARQAEARFKRLPHARKLLATCLAEPP"
     gene            305..550
                     /locus_tag="VIM45_01560"
     CDS             305..550
                     /locus_tag="VIM45_01560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8820005.1"
                     /translation="MTQHSDEQPTGPWWRPAVSPPPWWIWLLGIAADAGTGVWQLITT
                     NGTRDAGLFLGSMVWPGAAITAAVFVVVWLGWSLDLE"
     gene            561..1571
                     /gene="holA"
                     /locus_tag="VIM45_01565"
     CDS             561..1571
                     /gene="holA"
                     /locus_tag="VIM45_01565"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEY8820006.1"
                     /translation="MLRLYYGPDSYTRTAALAALRSELDGDGMLSANTAIFDGRSVKP
                     EELMSACDTVPFLAERRFVLVEGLLSSQETAPRTTRTGRSRSQAGSADDGWPWAGLAA
                     YARRLPESTELVCVDGELRVNNRLLAELRPLADVHVFPLMDREALSRWVSERVRERRA
                     SIQPRAAAVLAETVGANLWRMNSEVEKLSLFAYDRPIEVADVRHMVTVAPTGTVFQLV
                     DAAMAGQSGDALRLVRLLMEGGAAGPYLLTMLARQYRQLLLAQDLLRRRVSRDEIQRK
                     LELRYPGAMAKVMDQARRYPAARLESGYARLLEADLNIKRGIQDEETAIELLVAELAG
                     LR"
     gene            complement(1589..2587)
                     /locus_tag="VIM45_01570"
     CDS             complement(1589..2587)
                     /locus_tag="VIM45_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8820007.1"
                     /translation="MAQPILEVQDLSVEFATREGPVRAVNHASLALASGEIVAIVGES
                     GSGKTTLALSVLDLLPFPGRVTSGSIRFENQNLLSLRDDELRKLRGNAISMIFQDPVS
                     CLNPVLPIGRQVEEILTSHLKLDKKERKRRAISLLQSVGLADAERIANQYPFHLSGGM
                     CQRVMIAMATALNPRVIVADEPTSSLDVTVQAQILAELDGLRRTRNTAILLITHDFGV
                     VAQSADRVAVMYGGAIVEQGDVATVFSKPQHPYTWALLNTLPRLDGRNGRLRAIPGSP
                     PSLLNLTPECSFVTRCTKALSRCRIEPAPPLVEAGTNHAVACYNPIFQDWADDADD"
     gene            complement(2686..3327)
                     /gene="lexA"
                     /locus_tag="VIM45_01575"
     CDS             complement(2686..3327)
                     /gene="lexA"
                     /locus_tag="VIM45_01575"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015408062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEY8820008.1"
                     /translation="MKGRLSVKQQHILDYMRSFMDDNSYPPSIRDIQSGCSISSTSVV
                     DYNLKALERLGYIHRDREVSRAIELLDNGPKRMRVVSVPVVGLIAAGTPIPVPSADSF
                     AVDYSETVEVGDSVTRGRENVFALRVKGTSMIDAFINDGDVVLFEATDHAENGEMVAA
                     WLKREGETTLKKFYHEGDRVRLQPMNSTMGPIYTAADNVEVQGRLIYVTRSMV"
     gene            3507..>3795
                     /locus_tag="VIM45_01580"
     CDS             3507..>3795
                     /locus_tag="VIM45_01580"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY8820009.1"
                     /translation="MFGRHVILRPIVPGVLQVPLFPTSAFLLLGDRVTLVDTGFRGSG
                     VRLLHAVQAAGRRADEIERVIITHYHPDHLGGLAELQQVLPAKAAIHAVEAS"
BASE COUNT          666 a         1149 c         1240 g          740 t
ORIGIN      
        1 tggctgcgca ccgctggcag ttgtacctgc tccgctgccg cgacggcacg ctctacacgg
       61 gcatcagcac cgacgtggtc cgtcgtgtgc aggagcatga cacggggagg ggtgcgcgtt
      121 acacgcgcgg gcgctcaccc gtgcagttgc ttgccgcatg gccgtatgct gacgagtctg
      181 cagcccggca ggccgaagcg cgtttcaagc gactgcccca cgcgcgcaag cttttggcaa
      241 catgcttggc cgagccgccg tgagcgcaca ccacatacaa tgaccgggaa agggcccgcc
      301 tccgatgacg cagcactccg acgagcagcc taccggtccg tggtggcgac cagcagtttc
      361 tccaccgccg tggtggatct ggctgctcgg aatcgccgcc gatgccggta ccggtgtgtg
      421 gcagctcatc acgacgaacg gcacgcgcga tgccggcctg tttctgggca gcatggtgtg
      481 gccgggggcc gcgatcacag cggccgtgtt cgtcgtcgtg tggctcggct ggagcctgga
      541 cctggagtag tgatcaccga gtgttgcgcc tgtattacgg tcctgactcg tatacccgca
      601 ccgcagcact agcggcgttg cgaagtgagc tcgacggcga cggcatgctc tccgccaaca
      661 ccgcgatctt cgatggccgc tcggtgaagc cggaggaact tatgtccgcg tgcgatacag
      721 tgccgttcct ggccgagagg cggttcgtgc tcgtggaagg tttgctgagc tcacaggaaa
      781 ctgcgccgcg aaccacgcgg acgggccgtt ctcggtctca ggcgggttct gcggacgacg
      841 ggtggccctg ggcgggatta gcggcatacg cgcggcggct gccggaaagc accgagcttg
      901 tgtgtgtgga tggcgagctg cgggtgaaca accggctgct tgccgagcta cgcccgctgg
      961 ccgacgtgca cgtattcccc cttatggatc gagaggctct gtcgcgatgg gtatccgagc
     1021 gggtgcgaga gcggcgcgcg tccattcagc cgcgcgcggc ggcggtgttg gccgaaacgg
     1081 tgggtgcgaa cctctggcgt atgaacagcg aggtcgaaaa actcagtctg ttcgcttacg
     1141 accgccccat agaggtcgca gacgtccgtc acatggtgac ggttgcaccg acgggcaccg
     1201 tatttcaact ggtcgacgcg gcgatggccg gtcagagtgg cgatgccttg cggctcgtgc
     1261 gcctgctcat ggagggtggc gccgcggggc cttatctcct gacgatgcta gcgcgccagt
     1321 atcggcagtt gttgctggcg caagacttgc tccggcgtcg tgtgagtcgc gacgagattc
     1381 agcgcaagct cgagctccgg taccccggcg cgatggcgaa ggtgatggac caggcccgtc
     1441 gttacccggc ggcgcgcctt gaatctggct atgctcgcct gcttgaagcg gaccttaaca
     1501 tcaagcgcgg cattcaggat gaagagacgg cgatcgagct gctcgtcgct gagctggccg
     1561 ggctgcggtg aatcggtggc tgcattcgct aatcgtcggc gtcgtcggcc cagtcctgaa
     1621 agatcgggtt gtaacaggcg acggcgtggt tggtgccggc ttccaccagg ggcggggccg
     1681 gttcgatgcg gcagcggcta agcgccttgg tgcaccgggt cacgaagctg cattccggcg
     1741 tgaggttgag caggctgggc ggcgagccgg ggatcgcgcg aagccggccg ttgcgcccat
     1801 ccaggcgcgg cagcgtattg agcagcgccc aggtgtacgg atgctggggt ttgctgaaca
     1861 cggtcgccac gtcgccttgc tcgacgatcg cgccgccata catcaccgcc acgcggtcgg
     1921 cagactgcgc taccacgccg aagtcgtgtg ttatgagcag gatggcggtg tttcgcgttc
     1981 ggcgcagacc atccagttcg gccaaaatct gggcttgtac agtcacgtcc agcgatgacg
     2041 tcggttcgtc tgcgacgatt acgcgcgggt tcagggcggt agccatggcg atcatcaccc
     2101 gctggcacat gccgcccgat aggtggaacg ggtactggtt ggcgatgcgc tcggcgtccg
     2161 ctagaccgac gctttgcaac aggctgatag cgcgccgctt gcgttctttc ttgtcgagct
     2221 tgaggtggct cgtaaggatc tcttcgacct ggcgtccaat gggcaggacc gggttcaggc
     2281 agctcaccgg gtcctggaaa atcatcgaga tcgcgttgcc gcgcagtttg cgcaactcgt
     2341 cgtcgcgcag agatagaaga ttctgatttt caaaccgaat actgccgctg gtcacgcgac
     2401 ccggaaacgg caacaaatcc agcactgaca gcgccagcgt cgtcttccca ctgccactct
     2461 caccgacgat cgccacgatc tcgccggacg cgagtgccag cgacgcgtga ttcaccgccc
     2521 gcacggggcc ttcgcgcgtt gcgaactcga ccgagaggtc ttgaacttcg aggatgggct
     2581 gcgccatgcc gctctcgccg tgatatgcaa agtgagcggt agtagcctac cgctcacctg
     2641 tctgtggtcc aatcataccg cgaaggtcct gcgagctgct cagggctaca ccatcgaccg
     2701 cgtgacgtaa atgaggcgcc cctgaacctc gacgttgtcg gccgcggtgt agatgggccc
     2761 catcgtggag ttcatcggct gaaggcggac gcggtcacct tcgtgataga acttcttgag
     2821 cgtcgtctca ccttcgcgct tgagccaggc tgcaaccatc tcgccgtttt cggcgtgatc
     2881 ggtggcctca aacagcacga cgtctccatc gttaatgaaa gcgtcgatca tcgatgtccc
     2941 cttgacccga agagcaaaga cgttctctct gccgcgcgtg acgctatcgc caacttccac
     3001 ggtttccgag tagtcgacgg cgaagctatc agccgatggg acggggatcg gagtgccggc
     3061 ggcaattagt ccgactaccg gcaccgagac aacgcgcatg cgctttggcc cgttgtccag
     3121 gagctcgatc gcgcgggaaa cctcacggtc tcggtgaatg tatccaagcc gctcgagtgc
     3181 cttgaggttg taatcgacca ccgaggtaga actgatactg cagccggact ggatatcgcg
     3241 aatgctgggc ggatatgagt tgtcgtccat aaacgagcgc atgtagtcga gaatgtgctg
     3301 ctgctttacc gacagcctac ccttcaacgt gatgccccct gttcgctcga tgccggcgtg
     3361 attaccggct gtcgggtgcc aaaatcggaa cacccgttct ggacaacaaa tgtagcactc
     3421 actccataga gtgtcaacaa cgtcagcttt ccgtatttga ccggtttgag cttgacctcc
     3481 gacccatcgt gtgacgacga tccggtatgt ttggacgcca cgtcatcctc cgtccgatcg
     3541 tccccggagt ccttcaggtg ccgctgttcc ccacatcagc gttcctgttg ctcggggatc
     3601 gagtgaccct ggtagatacg ggcttccgtg gaagcggggt gcgattactg cacgccgttc
     3661 aggcagccgg acgccgggcc gatgagatcg agcgcgtgat catcacgcac tatcacccgg
     3721 atcacctcgg aggattggcg gagttgcaac aggtgctgcc ggcaaaggcg gcgatccatg
     3781 cagtcgaagc cagcg
//
LOCUS       DATCPE010000051         5489 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U7219
            k141_397704, whole genome shotgun sequence.
ACCESSION   DATCPE010000051 DATCPE010000000
VERSION     DATCPE010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955033
            Sequence Read Archive: SRR1586279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 5489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 16:49:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 640
            CDSs (total)                      :: 631
            Genes (coding)                    :: 627
            CDSs (with protein)               :: 627
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5489
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_397704"
                     /isolate="SMAG_U7219"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..918
                     /locus_tag="VIM45_01585"
     CDS             <1..918
                     /locus_tag="VIM45_01585"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEY8820010.1"
                     /translation="QVIPTPEVDRNDPVDREAAYAALQDLTRPLYYDHVVQPGDTVST
                     IAALYGISSDTVLWNNPLIVDRNALFIGQTLRLPAKNGVLYNVHYGDTLNDIADSYKV
                     EPTALLSLAANGLADAGSLKEGQTVLIVGGQPPAPPPPPPAVVTPSPTPPPQPSATPP
                     TLAASATRAPAPAPPPAASSTWIWPAHGPISSYFGPGHPLGIDIGQGAGGQPIIAARA
                     GTVTFAGGDACCSYGYYVDIDHGSGFSSRYGHFRSWPIVAVGQHVNQGQVLGYAGTTG
                     YSTGPHLHFEIHINGAIVNPLNYLPPGGN"
     gene            complement(937..1578)
                     /locus_tag="VIM45_01590"
     CDS             complement(937..1578)
                     /locus_tag="VIM45_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY8820011.1"
                     /translation="MELTWLGHACFRLKNRDTTIILDPYGPNCGYPSRNFGEADVVTV
                     SHNHAAHDNVGAVSKQRLVISGPGEYEVAGVLITGVRTFHDAMRGGERGANTAFVFEM
                     DELRICHLGDLGHVPTPQQAEALSGAEILLIPVGGHTTIDAAAAAEVVSLLEPRISVP
                     MHYRVDGSTVDLDPLDAFLKQMGVSAPQPSARLSVTRTSLPLETTVSVLEYRR"
     gene            complement(1604..1885)
                     /locus_tag="VIM45_01595"
     CDS             complement(1604..1885)
                     /locus_tag="VIM45_01595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8820012.1"
                     /translation="MKAASERTVTESTRRTKTVQGHPTQSVACDLGVTGKLLRSTLVT
                     RSAPPGFREALRRQLVDTAGGAIAGAERFGRFGAGSVSVANPRRHDSTA"
     gene            complement(1882..2466)
                     /locus_tag="VIM45_01600"
     CDS             complement(1882..2466)
                     /locus_tag="VIM45_01600"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY8820013.1"
                     /translation="MTGHFRALGGGTAALQRPPDGELVEQARIDREAFGALYERYVDR
                     IYSYLYYRTDSAADAEDLTARTFYQALSHLADFEERGLPFASWLFTIAHNLVANYYRE
                     RSRRPTAPLDSAGEPASPSPFAAAEDAADVRDAIATLSPERQHLILLKYVEGMSNAEI
                     GRAMNRSEGAVKSLLRRTLDEMRRNMERNRDVAS"
     gene            2591..3607
                     /gene="hrcA"
                     /locus_tag="VIM45_01605"
     CDS             2591..3607
                     /gene="hrcA"
                     /locus_tag="VIM45_01605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEY8820014.1"
                     /translation="MLTDRRAQVLRLIVSDYIDTATPVGSEHIVKRHRLELSPATIRN
                     EMAKLEEEGYISHPHTSAGRVPSDLGYRYYVEMLMEEPNVSNDEQLRILHQFHQSTAE
                     LSEWLRLAASVLAQELRNVAVVSAPRSTRSRLKHLELVSLHDHSALLVVVLQEVKVRQ
                     QVVALRETATQDDLSRLSRKLNHLFAALTASQISARTVDLLPLEEQIAGLVAEIVQTE
                     DVVEFPDPHLEGIRNVLSQPEFARSDRVLDILEALDERNIARTIPQADIGEDGVTVII
                     GAENPENVMRDLSIVLGRYGDSQGPSGMLAVVGPTRMPYSRAIATVRYVGSLMTELMM
                     KLYD"
     gene            3659..4246
                     /locus_tag="VIM45_01610"
     CDS             3659..4246
                     /locus_tag="VIM45_01610"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEY8820015.1"
                     /translation="MVSNEHHQPNDGSHRRRGRSGRSGEIKHDAQENGVKAEQDTETL
                     ESLQAQLSEERNRADGYLEQWKRATADYQNYKRRIEQEREEYGRLANTAILLNILPAV
                     DDLDRALANVDVPLSGQKWVEGVEAINRKLKGALEASGLAVIPAEGEAFDPNVHEAIS
                     EAPGEHGQVVAEVRPGYRLGSRVIRPALVVVGNGN"
     gene            4315..>5489
                     /gene="dnaK"
                     /locus_tag="VIM45_01615"
     CDS             4315..>5489
                     /gene="dnaK"
                     /locus_tag="VIM45_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007923368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEY8820016.1"
                     /translation="MAKVIGIDLGTTNSAVAVMEAGEPVIIPNAEGGRITPSMVALSK
                     TGERMVGQVAKRQSVTNPDNTIFSVKRLMGRKFDDVEVQRTIKMVPYKIVKGPNGDAH
                     VEMGGRSYSPPEISAMILQKLKTDAESYLGEKITEAVITVPAYFNDSQRNATKDAGKI
                     AGLEVLRIVNEPTAAALAYGLDKKHDETVAVYDLGGGTFDVSILELGEGTFQVKSTNG
                     DTHLGGDDFDQVLVNYLIDEFKKDQGIDLRNDRPALQRLREAAEKAKIELSTLLQTEV
                     NLPFITADQNGPKHLVITLTRSKLEQLTNDLVDKTLGPCRKAQEDASVSATQINEVVL
                     VGGQTRMPLVQEAVKKFFGKEPHKGVNPDEVVAIGAAIQAGVLRGEVKDVLLLDVTPL
                     "
BASE COUNT         1149 a         1702 c         1679 g          959 t
ORIGIN      
        1 caggtaatac cgacgcccga ggttgaccgc aacgatccgg tcgaccgcga agccgcatac
       61 gccgcactcc aggatctgac acggccgctg tactacgacc acgtggtaca gccgggcgat
      121 accgtttcca cgatcgccgc cctttatggg atatccagtg acacggtgct gtggaacaac
      181 ccgctgattg tagaccgaaa tgcgttattt atcggtcaaa cgctgcgcct gccggcaaag
      241 aacggcgttc tttacaacgt gcattatggc gacacgctca acgacatcgc cgactcgtac
      301 aaagtcgagc ccacggcgct gctttcgctg gccgccaacg gccttgcaga cgcgggttct
      361 ctcaaggaag gtcaaacagt cctgatcgtc ggtggccagc cgcccgctcc acctccgcca
      421 ccacctgccg tcgttacgcc gtcgccaact cccccaccgc agccatctgc tacgccgccc
      481 accctggccg catcggcgac gcgggcacct gcacccgcac cgccaccggc cgcttcatct
      541 acctggatct ggcctgctca cggaccgata tcaagttact tcggaccggg ccacccgctc
      601 gggatagaca tcggccaggg cgccggtggg cagccgatta ttgccgcccg agccggcacc
      661 gtcacgtttg ccgggggcga cgcgtgctgc agttacggat attacgtaga tatcgaccac
      721 ggcagcggat tcagctcgcg atacggccac ttccgaagct ggccgatcgt cgccgtcggg
      781 cagcacgtca accagggcca ggtgcttggg tatgccggca ccaccggcta cagcaccggc
      841 ccccacctgc acttcgaaat ccacatcaac ggcgccatcg tcaacccgtt gaactacttg
      901 ccgcccgggg ggaactagcc cgatcgttgg gcaacgttac cgccggtact cgagcaccga
      961 gacggtagtc tcgagcggga ggcttgtcct ggtgacgcta agccgcgcgg atggttgcgg
     1021 cgcgctgacg cccatttgct tgaggaaggc gtccagaggg tccaggtcca ccgtgcttcc
     1081 gtcgactcgg taatgcatag gcaccgatat tcggggctcg agcaggctaa cgacttcagc
     1141 cgcggccgcc gcgtcaatcg tggtgtgccc gcccacggga atgagcagga tttcggcgcc
     1201 gctcaacgcc tcagcctgct gcggcgtcgg tacgtgaccg aggtcgccca ggtgacaaat
     1261 acgcagctca tccatctcaa agacaaaggc ggtattcgcc cctctctccc caccgcgcat
     1321 ggcatcgtga aacgtgcgca cgccggtaat gagtacgccg gcgacctcgt attcgccggg
     1381 tccgcttatc acgagccgct gtttggatac ggcgccaacg ttgtcgtgag cggcatggtt
     1441 gtggcttacc gtcacgacgt cggcctcgcc aaaattgcgc gaagggtacc cgcaatttgg
     1501 accgtaagga tcaagaataa tcgtcgtatc gcgattcttc agtcgaaagc aggcatgacc
     1561 aagccaggtg agttccatat cgtctcctca cgcgcgttgc ggtctaggcg gtcgagtcgt
     1621 gccgccgcgg gtttgccaca gagaccgagc cggcaccgaa ccgaccaaag cgctctgccc
     1681 ctgcaatagc gccgccggca gtatcgacga gctgacggcg aagggcttca cggaatccgg
     1741 gcggcgccga gcgcgtcacg agggtactgc gcagtagttt gccggtgaca ccgaggtcgc
     1801 aggccaccga ttgagtcgga tgtccttgca cggttttcgt cctccgtgtc gattcggtca
     1861 cggtccgttc ggaagccgct ttcacgatgc gacgtctcga ttgcgttcca tgttcctgcg
     1921 catttcgtca agcgttcgtc gcaacagact tttcacggcg ccttcgctgc gattcatcgc
     1981 ccggccgatc tccgcgttgc tcattccttc gacgtatttt agcaaaatca ggtgttgacg
     2041 ctccggcgag agcgtcgcaa tcgcgtcgcg cacgtccgcc gcatcctcgg cagcggcaaa
     2101 cggcgacggc gacgcgggct ctccggcgct gtcgagcggc gccgtggggc ggcgcgaacg
     2161 ttcgcgataa tagttggcca ctaggttgtg ggcgatagtg aacagccacg aggcgaatgg
     2221 gagaccgcgc tcctcaaagt cggcaaggtg gctcaacgcc tggtagaacg tgcgggcggt
     2281 gaggtcctcg gcgtcggcgg cactgtcggt acggtaatag aggtagctgt aaattcggtc
     2341 gacgtagcgt tcgtacaacg cgccaaacgc ctcgcggtcg atgcgcgcct gttctacgag
     2401 ctcgccgtca ggcggccgct gcaacgccgc tgttccgcca ccgagcgctc gaaagtgacc
     2461 ggtcaccctt gacaatgaca cactcaactg ttactctcac tgggttagca ctctccgacc
     2521 ttgagtgcta acccagccga ttccattcaa tacttctgcc gacgatcggc cggctggagt
     2581 agaacacggc atgctgactg atagacgtgc gcaggtactg cgccttatcg tatccgacta
     2641 catcgatact gcaactcctg tgggttccga gcatatcgtc aagcgacacc gcctcgaact
     2701 gtctcccgct acgatccgta acgagatggc caaactggag gaggaaggat atatctcgca
     2761 cccgcacacg tcggcgggtc gggtgccctc cgatctcggt taccgctact acgtcgaaat
     2821 gctgatggag gagccgaacg tcagcaatga cgagcagctc cgcatcctgc accagtttca
     2881 ccagtcgacg gcagagctca gcgaatggct gcggttggcg gcttccgtgc tcgctcagga
     2941 gttgcgaaac gtcgcggtgg tttcggcgcc gcgctcgacc cgctcacgcc tgaagcacct
     3001 cgaattggtc agtctgcacg accacagcgc cctgctggta gtggttctgc aggaggtcaa
     3061 agtacgccag caggtcgtgg cgttgcgcga gacagcgacg caggacgacc tcagccgctt
     3121 atcgcgcaag ctgaatcacc tgttcgctgc cctgacagcc agccagattt cggcccgcac
     3181 cgtcgatctg ttgccgcttg aggagcaaat tgccggactg gtcgcagaaa tcgtccagac
     3241 agaggatgtg gtcgaattcc cggacccaca ccttgagggt atacggaacg tcttatcgca
     3301 gccggagttc gcccgtagcg accgcgttct cgacattctc gaggcgctgg acgaacgcaa
     3361 tatcgcccgg acgatcccgc aggccgacat cggcgaggac ggagtaaccg tgatcatcgg
     3421 ggccgagaac cccgaaaacg tgatgcgaga cctaagcatc gtcctcggcc gctacggcga
     3481 ttcgcagggg ccatccggca tgctcgccgt ggtcgggccg acgcgaatgc cctattcacg
     3541 ggcgatcgca acggtgcgct acgtcgggtc gttgatgacc gaactcatga tgaagctgta
     3601 cgactgaact tgccacgagc ggccagatat ccgatttcgc gtgcatccag gagcagcaat
     3661 ggtttcaaat gaacatcatc aaccgaatga cgggagtcat cgccgacgcg gccgatccgg
     3721 tcgctccggc gagatcaaac acgacgcgca ggaaaacggc gtcaaagcag aacaagacac
     3781 cgagactctt gagagcctcc aggcgcagtt gagcgaggag cgcaaccgcg cggacgggta
     3841 tttggagcaa tggaagcgag caacggccga ctatcagaac tataagcgcc gcattgagca
     3901 ggagcgagaa gagtacgggc gactcgccaa tactgctatt ctgctcaaca tcttgccagc
     3961 agttgacgac ctcgaccgcg cgttggcgaa cgtcgatgtc ccgctctccg gccagaagtg
     4021 ggtcgagggc gtcgaggcga ttaaccggaa gctcaaaggc gccctggagg cgtcgggatt
     4081 ggcggtcata ccagcagaag gcgaggcgtt cgatcccaac gtgcacgaag ccatcagcga
     4141 ggcccccggc gaacacggcc aggttgttgc ggaggttcgg ccaggttaca ggctcggcag
     4201 ccgcgtcatc cggccagcgt tggtggtcgt cggcaacggc aattaagacg gataaccagt
     4261 agaaaaacgg ggctcgattt ctaagcagaa gaggtccagg agggaagtca gatcatggcg
     4321 aaggtcattg gcatagatct cggtacgaca aatagtgcgg tcgcggtgat ggaagccggc
     4381 gagccggtca tcatccccaa cgccgaaggc gggcgcatta caccctcgat ggtggcgctc
     4441 agcaagaccg gcgaacggat ggtcggacag gtggcaaagc gccagtccgt gaccaacccg
     4501 gacaatacga tcttctcggt gaagcggctg atgggccgca agttcgacga cgtggaagta
     4561 cagcgcacca taaagatggt gccatacaag atcgtcaagg gaccgaacgg cgatgcgcac
     4621 gtcgagatgg gcggccgcag ctactcgccg ccggaaatct ccgcgatgat cctgcagaag
     4681 ctcaagacgg acgcggaatc gtacctgggc gagaagatta ccgaagccgt cataaccgtg
     4741 ccggcgtact tcaacgacag tcagcgcaac gccacaaaag acgccggcaa gatcgccgga
     4801 ctggaagtgt tacgcatcgt caacgagccg acggcagcag cgctggccta cggcctcgat
     4861 aagaagcacg acgagacggt cgcggtctac gacctcggtg gcggaacgtt cgacgtctcg
     4921 atcttggagc ttggagaagg cacgttccag gtaaagagca ctaacggcga tacgcacctt
     4981 ggtggcgacg atttcgacca ggtcctggtg aactacctga tcgacgagtt caagaaggac
     5041 caggggatcg acctgcggaa tgaccgtccg gcgctgcagc gcctgcgcga ggcggccgag
     5101 aaagcgaaga tcgagctctc gacgctgctg cagacagaag tgaacctgcc gttcataacc
     5161 gccgaccaga acggtcccaa gcacctcgtg attaccctca cccgatcgaa gctggagcaa
     5221 ctgacaaacg atctggtgga caagacgctc ggcccgtgcc gcaaggcgca ggaagacgcg
     5281 agtgtgtctg caactcagat caacgaggtt gtactggtcg gcggccagac ccgcatgccg
     5341 ctcgtccagg aggcggtcaa gaagttcttc ggcaaggagc cgcacaaggg cgtcaacccg
     5401 gacgaagttg tcgccatcgg cgccgcgatc caggcgggcg tcttgcgcgg cgaagtcaag
     5461 gatgtactac tgctcgacgt gaccccgct
//
LOCUS       DATCPE010000052         6340 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U7219
            k141_268080, whole genome shotgun sequence.
ACCESSION   DATCPE010000052 DATCPE010000000
VERSION     DATCPE010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955033
            Sequence Read Archive: SRR1586279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 6340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 16:49:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 640
            CDSs (total)                      :: 631
            Genes (coding)                    :: 627
            CDSs (with protein)               :: 627
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6340
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268080"
                     /isolate="SMAG_U7219"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            216..983
                     /locus_tag="VIM45_01620"
     CDS             216..983
                     /locus_tag="VIM45_01620"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEY8820017.1"
                     /translation="MNLARVKNALTGPAANWDIRYEAEVSSTMDVARELAIQGALHGT
                     VALAGAQTSGRGRLGRSWVSEPDTNLYFTVVLRPSLTLLSRLAMVTPLAVAEAIEQVT
                     KLRAELKWPNDVQIDGLKCCGVLIDSEVQGSLPRFCLVGIGINVNSDPDGLPELAGIA
                     TSIANCAGHKISREDLLGAVLSRLGAHCMAVESGTPVQHLWKSRLNTIGRSVNVQSGA
                     DVHSGVVEDVTEEGTLKLRRDDGSIVSIPAGEVTLRA"
     gene            1051..2292
                     /locus_tag="VIM45_01625"
     CDS             1051..2292
                     /locus_tag="VIM45_01625"
                     /inference="COORDINATES: protein motif:HMM:NF024785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HEY8820018.1"
                     /translation="MIETAESLLCRGMRDLLPDEMVRFRRIEQTFRKACQSWGYSEIR
                     TPTLEHLYLFTSFGTLSPHLLGRVYSFLDWDGWTGERVVLRPDATIPVARLFREQLRS
                     SPAKLAYVTNIFRFAQGDEPREVWQCGAELIGESWPYGDLEQIAMALHIVRSAGVRNA
                     TISLSHSGVVRALLERAGFASDEQAELYDRLLDGDRNVVADVEARLPDLGPGLRLLSD
                     VGEGDSAFLANLRSAFLPGVPEMAVPLDELDLIALTATAFGASVTVSVTEVRDFEYYT
                     GPVFRLVSGDVTLASGGRYDRLITDANGGPLAACGFALHVDALLPLVQPPADSSHGII
                     QVTPADMSAKTLSTALVVAEQLQNAGFRAALVDAEPACRWCLTVDARAGQPHFRFDDR
                     ERGNRVEAHSIDQVIAAMRRS"
     gene            2296..3735
                     /gene="hisG"
                     /locus_tag="VIM45_01630"
     CDS             2296..3735
                     /gene="hisG"
                     /locus_tag="VIM45_01630"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEY8820019.1"
                     /translation="MAKIALPTGDLREPAAALLAEAGLPNLEYASGSRSLRLTLECAG
                     DAALRVFRERDIPIQIALGNYDIGICGLASIEELRLRFPQDEIVMLRPLPFGNVEVHL
                     ATDPETARCLGPMQTWPEHIGLRIVSDLPTIAESFALSARLPRARVTAVFGSADAYPP
                     EDAELAVLQASSAASVEQRGLVSMATLLTGSAWLIANRRSLAYQDLSAVLGPLLRLPP
                     SSTHGFGLNLPRLRSALQAALWHGWQRDAAVLRLALPDGHQQRHTYESLKEAGFRFDG
                     YTEKTYVRRPKSGVPGLDVKVIRPQDMPAQVALGKFDLAMTGRDVLRDHLLAFPDSPV
                     EEVVDLGRSGYGIAAVVDEGLPATTIREALDFWRRSGRKSIRIASEYPNIADNYARTH
                     HFGRYRVMPIAGASEAFVPEDAEVLIEGSETGKSIVANKLKIIDSLFESTNCMIAGRR
                     ALAEPRRALSQQIIAALRRAVAMSAEGQS"
     gene            3757..4494
                     /locus_tag="VIM45_01635"
     CDS             3757..4494
                     /locus_tag="VIM45_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HEY8820020.1"
                     /translation="MATARDIGRDLGSDLVDGESGAYYLGATTPDIRVLTRGNRRDTH
                     FFDLDSLEHQDSVEEFFRTHKHLADADKLDPQAAAFVSGYVTHLVLDESYIVSMYRPF
                     FGQLSSLGGDLNANMMDRLLQYELDRRRREAGHEVDEIREALAGCSLSLNVGFLDSAT
                     LNRWLEVAIDQTRHPPDWNRFRNQGGRHLGQDWDENPRAYEEFLKTIPDLLQRTIEHV
                     STAEVDAFLEASKERAQRIVERYLGCG"
     gene            4485..5816
                     /gene="hisD"
                     /locus_tag="VIM45_01640"
     CDS             4485..5816
                     /gene="hisD"
                     /locus_tag="VIM45_01640"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012189560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEY8820021.1"
                     /translation="MRIVTGKDEARRTILRRTALVSVEIPEHLRSRDRALFGPGLSVE
                     ETVRRIVDAVRDEGDTALQRFNVALDGALERPIRVTRAEIEAAYGDPAITPELVNALN
                     EAATRIRAYHELQLNYSARNFHESGLGQIVRPLKRAGVYMPGSAIIYPSSLLMTAIPA
                     SVAGVDEIYVCCSSGPDGKVPAIKLVAADIAGCTGVFATGGAQAIAAMAYGTESVPRV
                     DKICGPGNIFVTLAKRQIIGVTGIDALYGPTETVVIADDTADPILCAADLIAQAEHDE
                     IASPILITNSRGLAEKVAAEVERQVKLIPRGTVAAAAFENRGGAVIAADLDEAFDLAN
                     EYAPEHMCLLMRDAWSFVDKVRNAGGLFVGESSPEALADYIAGPSHVMPTGGSARYSS
                     PLTVSDFLKVTSVVALGDADLEALGPSAARIAHAEGLSGHARAIEVRLESR"
     gene            5816..>6340
                     /locus_tag="VIM45_01645"
     CDS             5816..>6340
                     /locus_tag="VIM45_01645"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY8820022.1"
                     /translation="MPANERSVDLMGLVRPEVRALPGYEAVEPVELLAERLGIDRERI
                     AKLDGNENPYGPSPRALEALSSYRDYNIYPDPDQRRLRAALSKHLGVPPEYIVAGSGS
                     DELIQLLVSVFLRPGDTVIDLVPTFGMYSFEADVAGGQTVQVKRRQDFSVDVNAVAEA
                     IDARTRLIFATSPNN"
BASE COUNT         1147 a         1934 c         2008 g         1251 t
ORIGIN      
        1 ctgctaacgt tagcgtcatt gaaaggaggt gatgtggatg gatagtttta gttgtagggg
       61 cctcgggccc gtccgtttgc atcgcagctc ccacggggta gctgcggtat agctccagtc
      121 gggttccgag gtcctcgcaa ctcgctcgat gggccagggg caaccgcccc tggccttaat
      181 ctttgtccgg aaaaacgccg cagatggatg tggcaatgaa tctcgcgcgt gtgaagaatg
      241 ccctgaccgg gccggcggcg aactgggaca tccgctacga ggccgaagtt agctcgacga
      301 tggacgtcgc gcgagagctt gcaatacagg gagcgctgca cggcaccgtc gcgctggccg
      361 gagcgcaaac atccggccgc gggcggctgg gaaggagttg ggtttccgag cccgacacca
      421 acctgtactt taccgtcgta ctacgcccgt cgctgacctt gctttcacgg ctggcgatgg
      481 tgaccccgct ggcggtggct gaggcgatcg aacaggtcac caagctccgg gcggaactca
      541 agtggcccaa cgacgttcag atcgatggcc tcaagtgttg cggcgtgttg atcgattctg
      601 aagtgcaagg cagccttccc cgcttctgtc tcgtgggcat cggcattaac gtcaactccg
      661 acccggatgg cttgcccgaa ctggccggaa ttgcgaccag cattgcaaac tgcgcgggac
      721 acaaaattag ccgggaagat ctacttgggg ccgttctctc gcggttgggg gcgcactgta
      781 tggctgtcga gtccggaacc ccggtgcagc acctctggaa gtcgcgcctg aacacgatcg
      841 gccgatccgt caacgttcag agcggtgcgg atgttcactc cggcgttgtc gaagacgtaa
      901 ccgaagaagg aacgctgaag ctgcgccggg acgacggctc gatcgtttcg attcctgccg
      961 gcgaagtgac gctccgggcc tgacgcggtt gccgctctaa aatggcagac gatagaataa
     1021 ttggacgaga tttatgcgag tcctaccttc atgattgaga ccgcggagag tttattgtgt
     1081 cgtggcatgc gcgatctgct gccggacgaa atggttcgtt tccggcgcat tgagcagacg
     1141 tttcgtaaag cttgtcaatc gtggggctat tcagagattc ggacgccgac actcgagcat
     1201 ctctatcttt tcaccagttt cggcacgctt tcaccgcatt tattaggtcg agtttattct
     1261 tttctcgatt gggatggctg gacgggcgag cgtgtcgtac tgcggcccga cgctaccatt
     1321 ccggtcgcgc gactatttcg tgaacaactc cgtagctcgc ctgccaaact tgcgtatgtc
     1381 accaatattt ttcggtttgc ccagggtgat gaaccgcgcg aggtgtggca gtgcggcgcg
     1441 gagttgatcg gtgagagctg gccgtacggc gatctcgagc agatcgctat ggccctgcac
     1501 atcgtccggt cggccggcgt tagaaacgcc accatcagcc tttcacactc cggcgttgtc
     1561 cgggcgctgc tcgagcgggc gggctttgcg tccgatgagc aagccgagct gtatgaccgg
     1621 ctgcttgacg gcgaccgcaa cgtcgtcgcc gacgtggaag cacggcttcc ggacctgggc
     1681 cccggcctgc gcctcctttc cgacgtcggc gaaggtgaca gcgcgtttct cgcgaacctg
     1741 cgaagcgcct ttctgcctgg cgttccggaa atggccgtgc cgctggatga gctcgatctg
     1801 atcgccctca ccgcgaccgc cttcggcgcg agcgtaacgg tgagcgtgac cgaggtccgg
     1861 gacttcgagt actatacggg ccctgtattt cggctcgttt ccggcgatgt gacgctcgcg
     1921 agcggtggac gttacgatcg actgattacc gacgccaatg gaggacctct cgctgcttgc
     1981 ggctttgcac tccacgtgga tgcacttctg ccgttggtgc agccgccggc tgattcatcg
     2041 catggaatta tccaggtgac gcccgcggat atgtccgcga agacgctctc cacggcgttg
     2101 gttgtcgccg agcaactcca aaatgccggc ttccgagcgg cgttggttga cgcggagccg
     2161 gcctgccgct ggtgcttgac tgtggacgcc cgcgccgggc aaccccactt tcgcttcgac
     2221 gaccgtgagc ggggtaatcg cgtcgaagca cactcaatcg accaggtgat cgcggcaatg
     2281 cggaggtcat agactatggc caaaatcgca ttaccgaccg gcgatcttcg tgaaccggcg
     2341 gctgcattgc tcgccgaagc ggggctgccg aacctcgagt atgcttctgg ttcgcgctca
     2401 ctgaggctca cgctggagtg tgcaggcgat gcggcgcttc gcgtcttccg tgagcgggac
     2461 atcccgattc agatcgcgct cggcaactat gatatcggca tctgcgggct cgcctcgatc
     2521 gaggagctgc ggttgcgctt tccgcaggac gagatcgtca tgctgcgacc gctgccgttc
     2581 ggcaatgtcg aggtccacct ggcaaccgat ccggagaccg cgcgctgtct cggtccgatg
     2641 cagacgtggc ccgaacacat aggtcttcgg atcgtcagtg accttcccac gatcgctgaa
     2701 agcttcgcgc tatcggcgcg cctacctcga gcccgcgtga ccgcggtgtt tggttcggcc
     2761 gatgcttacc cgcccgagga tgcagagctc gcagtactcc aggcttcgtc tgcggcttcg
     2821 gttgaacagc gcgggctcgt ttcaatggcg acgttgttga ccggtagcgc gtggttaatc
     2881 gccaatcgcc gtagcctggc ataccaagac cttagcgctg tcctcggtcc gctgctgcgc
     2941 ctaccgccgt cctcgacgca cggtttcggg cttaatctgc cgcggctccg ctccgcgctg
     3001 caggcggctc tgtggcacgg ctggcaacgc gatgccgcgg tgcttcggct ggccctgccc
     3061 gatggccacc agcagcgaca tacctacgag agtctcaaag aggcgggatt ccgcttcgac
     3121 gggtataccg aaaaaaccta cgtgcgacgc ccgaaatccg gcgttccggg tctcgacgtc
     3181 aaagtgattc ggccgcagga catgccggcc caggttgcgc tcggcaagtt tgacctcgca
     3241 atgaccggcc gtgacgtgct gcgcgatcac ctgctcgcgt ttcccgatag ccccgtagaa
     3301 gaagtagtgg acctcggtcg ctcgggctac ggcatcgctg ccgttgtcga cgaaggcctg
     3361 ccggcgacga cgatccgcga ggcgctcgac ttctggcggc gtagcggtcg caagagcatc
     3421 cgcatcgctt cagagtatcc gaacatcgcc gacaactacg ctcgaactca ccacttcggc
     3481 cgctatcggg tgatgccgat cgcgggcgcc tccgaagcgt tcgtgcccga ggacgcggaa
     3541 gtcctgatcg aaggctctga aacgggcaag tcgatcgtcg ccaacaagct caagatcatt
     3601 gattccctct tcgaatcgac caactgcatg atcgccggac gccgcgcgct cgcggaaccg
     3661 cggcgagcgt tgtcgcagca gatcatcgcg gcgctcaggc gagcggttgc catgtctgca
     3721 gaggggcaaa gctaagtgcc tccgattttc atgcacatgg ccacagccag ggatattggc
     3781 cgcgacctcg gctccgatct cgtcgatggt gaaagcggcg cctactacct cggcgccacg
     3841 acgcccgaca ttcgtgtcct gacgcgaggc aaccggcgcg atacgcattt tttcgatctc
     3901 gactcgctgg agcaccagga cagtgtcgag gagtttttcc gcactcacaa gcacctggcc
     3961 gatgcggaca agctggatcc gcaggcagcc gcatttgttt ccggctatgt tacgcacctc
     4021 gtgctcgacg agtcttatat cgtttcgatg taccggccgt tcttcggcca gctttcgtcg
     4081 ctcggtggcg acctcaacgc gaacatgatg gaccggctgc tccagtacga gctcgaccgg
     4141 cggcgtcgcg aggccggcca cgaagttgac gagattcgag aggcgcttgc cgggtgttcg
     4201 ctctcgctga acgtgggttt tctggatagc gcgacgctga atcgctggtt ggaggtcgcg
     4261 atcgaccaga cgcggcaccc gccagattgg aaccgcttcc gcaatcaggg cgggcgacat
     4321 ctcggccagg actgggacga aaacccgcgt gcctacgaag agttcctgaa gacaattccc
     4381 gatcttctgc agcgcaccat tgagcatgtg tcgacggcag aagtcgacgc cttcttggag
     4441 gcgtccaaag agcgtgctca gcggattgtc gaaaggtacc tgggttgcgg atagtaacag
     4501 gcaaagatga ggcgcgtcgc acaattctgc gtcgaactgc cctcgtctca gtagagatac
     4561 cggagcatct gcgcagccgg gacagggcgc tgttcggccc gggtctttcg gtggaagaga
     4621 cggtgaggcg gatcgtcgac gctgtacgcg acgaaggcga tacggccctg cagcgcttca
     4681 acgttgcgct cgacggggct ctggagcgtc ccatccgtgt cacccgcgcc gagattgaag
     4741 ccgcgtacgg cgacccagct atcacccccg agcttgtcaa cgccctgaac gaggcagcga
     4801 cccgaatccg cgcctatcac gagttacaac tcaattattc ggcgcgaaac ttccacgagt
     4861 cggggctggg gcagatagtg cgtcccctga agcgcgccgg cgtgtacatg cccggtagtg
     4921 cgatcatcta tccctcatcg cttctgatga cggccatacc cgcatcggtg gcaggcgtcg
     4981 acgaaatcta cgtgtgttgc tcgtccggtc ccgacggcaa agtgccagcg atcaagctcg
     5041 tggccgccga tatcgcgggc tgcacgggag tattcgccac cggcggggcg caggcgatcg
     5101 cggccatggc ctacggcacc gaatcggtgc cgcgcgtgga taagatctgc ggtccaggca
     5161 acattttcgt gacgctcgcc aagcgccaga tcatcggcgt caccggcatc gacgcgctct
     5221 acggaccgac ggagacggtc gtgattgctg acgacacggc cgacccgatc ctatgcgctg
     5281 cagacctcat tgctcaggcg gaacacgacg agatcgccag cccgattctg attaccaact
     5341 ctcgtgggct tgctgaaaaa gtggcggccg aggtcgagcg tcaggtcaaa ctgattcctc
     5401 ggggcaccgt cgcggccgct gcctttgaga accgcggcgg agcggtgatc gctgccgact
     5461 tggacgaagc gttcgatctc gccaacgagt atgcgccgga acacatgtgc ctattgatgc
     5521 gtgacgcatg gagcttcgtg gataaagtcc gtaacgccgg aggcctgttc gtcggcgaat
     5581 cctcgccgga ggcgctggcc gactacatcg ccggacccag ccacgtcatg cccacgggcg
     5641 gcagcgcacg ctattcgtcc ccgctgactg tgtcggactt tctcaaagtt acgagtgtgg
     5701 tggcgttggg cgacgccgac ctcgaggcgc tgggcccttc ggcagcccgc atcgcgcacg
     5761 ctgaagggct cagcggccac gcccgcgcca ttgaagtgcg gctggagtcg cgctgatgcc
     5821 ggcaaatgaa cgctcggtgg acctgatggg tctggtgcgg ccggaggtgc gcgcgttacc
     5881 gggttatgaa gccgttgagc cggttgagct tctggcggag cggcttggca tcgaccggga
     5941 acgaatcgcg aaactggacg ggaacgaaaa tccctacggc ccgtcgccgc gggcgttgga
     6001 ggcgctgtcg agctaccggg actacaacat ctacccggat cccgaccagc gccgtctgcg
     6061 cgcagcgctt tccaagcacc tgggagtgcc accggaatac atcgtggcgg gttcgggcag
     6121 cgacgaattg atccagttgc tggtatccgt ttttttgcga cccggcgaca cggtcatcga
     6181 ccttgtcccg actttcggaa tgtactcgtt tgaggcggac gtggccggcg gccaaacagt
     6241 acaagtgaag cgcaggcaag acttcagcgt cgacgtgaac gcggtcgcgg aagcgatcga
     6301 tgcccgcacg cggcttatct tcgcgacgtc gccgaacaat
//
LOCUS       DATCPE010000053         6592 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U7219
            k141_592437, whole genome shotgun sequence.
ACCESSION   DATCPE010000053 DATCPE010000000
VERSION     DATCPE010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955033
            Sequence Read Archive: SRR1586279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 6592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 16:49:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 640
            CDSs (total)                      :: 631
            Genes (coding)                    :: 627
            CDSs (with protein)               :: 627
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6592
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_592437"
                     /isolate="SMAG_U7219"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1599)
                     /locus_tag="VIM45_01650"
     CDS             complement(<1..1599)
                     /locus_tag="VIM45_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010937098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HEY8820023.1"
                     /translation="MMRQDRFTEQAQEVLAASQELMRQQRHTQWDVEHVLLSLVKREG
                     GLARQILERFRVPLDELTQTLETHLNAAPKSAFPAVQAYVTPRLVRMLKNADAEANRL
                     KDEYIGVEHLLIAISDEREGESARILRTFGVDKERIYRALQEIRGTHRVDNPRAESKY
                     QALNKYATDLTELAREGKLDPVIGRETEVKRVMQILNRRTKNNPVIIGEAGVGKTAIV
                     EGLAQKIVLGDVPDNLQDRRLLALDMGALVAGSRFRGEFEERLQAVMAEVRQAAGEIV
                     LFIDELHTVVGAGAAEGSIDASNMLKPALARGELRAIGATTLDEYRQHIERDPALERR
                     FSPVYVDEPSVEETVAILKGLRTRYEEHHKVKISDEAISAAATLSDRYVTERFLPDKA
                     IDLIDEAASKHVIEAESLSPELQSMKLRLDQLSRETDAAVQAEDYEDAARIKSDLLRL
                     QEDYAKAKEEWQAKRPRKTEIDEADIAELIASITGVPVSRMLEGEAAKLLDMEQRLHE
                     RVIGQDAAISVLADAIRRARAGLKD"
     gene            complement(1625..1972)
                     /locus_tag="VIM45_01655"
     CDS             complement(1625..1972)
                     /locus_tag="VIM45_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010937097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY8820024.1"
                     /translation="MAQDSIQNEDPCYVISIAARMVGMHQQTLRYYERVGLIEPSRTR
                     GNIRLYSPANINRLRQIQRLISDLGVNLAGVEVIINMGQRLQSLEAEIQQLRAEVEHC
                     RTQHQQVRGRASS"
     gene            complement(2033..3052)
                     /locus_tag="VIM45_01660"
     CDS             complement(2033..3052)
                     /locus_tag="VIM45_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012876226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HEY8820025.1"
                     /translation="MPTMNYYDILGVKRDATNKEIRAAFRKLARKFHPDVNPGDKTAE
                     AKFKEINAANEVLSDAEKRRKYDKFGDKWEYSDQIEEQQRQQASAGQWFRTAGRGTRT
                     TGGFGGVETDLGDLGDIFGNVFRGRSKPVARKGENLEHPLDVSLEEAYRGTTRTLTIQ
                     NNEPCVGCKGTGLAGEAICRVCDGVGYVTRPRKLEVKIPAGVKIGSRVRIAGEGQPGT
                     GGGSKGDLYLLINVPPSERFERKGDDLYEDVDVPLYDALLGGEAPVLTMTGRVMLKIP
                     AGSQNGKTIRLTGKGMPRLGGSGQGDLYARIRVMLPDRLSPPEQELFEQLRALRQPEA
                     AHASR"
     gene            3320..4309
                     /locus_tag="VIM45_01665"
     CDS             3320..4309
                     /locus_tag="VIM45_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEY8820026.1"
                     /translation="MVQETTRSSEQIREQSRFLTEVRSEVAKVIVGQQYLISRLLIAL
                     LTGNHVLIEGVPGLAKTLSVKTLSDALHLAFVRVQFTPDMLPADLTGTTIYNARDAQF
                     TVRQGPIFANIVLADEINRSPAKVQSALLEAMQERQVSIGGETFPLPAPFMVLATQNP
                     IESEGTYPLPEAQLDRFMIKAVIGYPSRLEERAILDRGISGELGEVRRIVSPEMILAA
                     RAMVREVYLDDRLKDYIIQIISATRNPRAFRMDTLAPFIAYGASPRATIFLAQAAQAH
                     AFVDGRSYVTPDDIKEIAPDVLRHRLVVTYEAEAEEVSPDEIVKRVLAGVDVP"
     gene            4345..5292
                     /locus_tag="VIM45_01670"
     CDS             4345..5292
                     /locus_tag="VIM45_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEY8820027.1"
                     /translation="MAETNEPVVPGGGLTSELLRKVKRIEIRSRRLVNNLFVGQYHAV
                     FRGRGIEFSEVREYSPGDEVRTIDWNVTARLGTPFIKKFVEERELSVLLLVDVSASGA
                     FGTVAQTKREVAAEIAALLALSASDNQDRVGLLAFTNEIEKYIVPAKGQRHVLRLVRE
                     LLALQPLNRGTRIGAAVDYVSRILKRRSVVFIISDFCDSGDYERPLRVLGQKHDVIAV
                     ALADPRDEELPNIGLLELEDAESGETVLVDTSDARVRRHYRATVEAARAERQRIFRTN
                     SIDAIEINTAASYVEPVLAFFRRREGSGGTGRGRRALSA"
     gene            5289..6293
                     /locus_tag="VIM45_01675"
     CDS             5289..6293
                     /locus_tag="VIM45_01675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8820028.1"
                     /translation="MNARAFRLLLFIVCLSAGLASVRLTQLSAAAAGEQQDNQTPDVT
                     VNIDRARATVGDPISLTVTIKHLANVTIDTTSIDDQLGSLEPIASDPPDDRPAGGGME
                     LRLRYKLASYHTGTVELPVLTFTYTLPDGTQAQVQSKAPVPITIQSVLPDGADPTDIR
                     PLKQQFSLPVPASEQLLWIIGGGGIPVAFVVVAVLSYLLIWRKRPPQSIAARHATQAA
                     RADLDRVMGLGLLERGELLDHYRQLANCVRRYLTERYGFPAVALTTGELERQMEARGI
                     ARWPARLISGVLSECDAVVYARYQPAAVRSEADNAMAYEILDATDDAGVAPKPMVQAV
                     "
BASE COUNT         1161 a         2054 c         1975 g         1402 t
ORIGIN      
        1 gtccttcagg ccggcacgcg cccggcggat cgcgtcggcc aggacgctga tggcggcatc
       61 ctggccgatg acgcgctcgt ggaggcgctg ctccatatcg agcagttttg ctgcctcgcc
      121 ttcgagcatg cggctcaccg gaacaccggt tatactcgcg attagctcgg cgatgtccgc
      181 ttcgtcgatt tccgttttgc gcggacgctt agcctgccac tcttccttgg ccttggcgta
      241 atcctcctgg agccgcagca agtcgctctt gatccgtgcc gcgtcctcgt agtcctcggc
      301 ctgcacggcg gcgtccgttt cgcgcgaaag ctggtcgagc cgcaacttca tcgattgcag
      361 ctccgggctc aagctttccg cttcgataac gtgtttgctc gcggcctcgt cgatgaggtc
      421 gatcgccttg tcgggcagga atcgctccgt gacgtaccga tcggatagcg tcgccgcagc
      481 actgatcgcc tcgtccgata tcttgacctt atggtgctct tcgtacctcg tccgcagccc
      541 cttcaggatg gcgaccgttt cctcgacgct cggttcatcc acgtacaccg gggaaaagcg
      601 ccgctccagc gccgggtcgc gctcgatgtg ctggcggtat tcgtctagcg tggtcgcgcc
      661 gatcgcccgc agctctcccc gggcaagcgc cggcttcaac atgttgctgg cgtcgataga
      721 cccttccgcc gcgccggctc cgacgacggt gtggagctcg tcgataaaca gcacgatctc
      781 tccggctgcc tggcgcacct cggccatcac ggcctgcagc cgttcttcga attcgccacg
      841 gaagcgtgag ccggccacca gggcgcccat atccaatgcc agtaggcggc gatcctggag
      901 gttgtcgggc acatcgccca acacgatctt ctgcgcaagg ccctcgacga tcgcggtctt
      961 gcccacgccc gcctccccga tgatcaccgg gttgttcttg gtgcggcgat tgaggatctg
     1021 catcacccgc tttacctcgg tctcccgacc gattacgggg tcgagcttgc cttcgcgggc
     1081 cagttcggtc aggtccgtcg catacttgtt gagggcctga tacttgcttt cagcgcgcgg
     1141 gttatcgacg cggtgggtcc cgcgaatctc ctgcaatgcc cgataaatcc gttctttgtc
     1201 cacgccgaac gtgcgcaaaa tacgggccga ttcgccctcg cgttcatcgc tgatcgcaat
     1261 cagcaagtgc tcgacaccga tatattcatc cttgagacgg tttgcttcgg cgtctgcatt
     1321 tttcagcatc cgaaccaatc tcggcgtaac gtacgcctgc acagccggaa aggccgactt
     1381 gggtgccgcg ttcaagtgcg tctcaagtgt ctgcgtaagc tcgtcaaggg gaacgcgaaa
     1441 tcgctcgagg atctgccggg cgaggccgcc ttcacgtttg actagggata gcagcacgtg
     1501 ctccacatcc cactgagtat ggcgctgctg gcgcatcagc tcctgggagg cggccagcac
     1561 ctcctgtgcc tgctctgtaa atctgtcttg acgcatcatg gggtaatttt cacctatcgc
     1621 tggttcagct tgacgccctg ccacgcactt gttgatgttg tgtgcggcaa tgttcgacct
     1681 ctgcgcggag ttgttggatc tccgcttcga gactctgtaa tcgctggccc atgttaatga
     1741 tcacctcgac gccggccagg ttgacgccga ggtcgctgat gagccgctgt atctgtcgta
     1801 atcgattgat gttggccggc gaatacagcc tgatgtttcc gcgcgtgcga gacggctcga
     1861 tcaacccaac gcgctcgtag taccgcaacg tctgctggtg catccccacc attcgcgccg
     1921 cgatgctaat gacgtagcaa ggatcctcat tctgtataga atcctgcgcc atcaatgtct
     1981 gcctcctatc tggcatctcg tctgcccacc cggtgcggcg ggtcagattg gatcacctgg
     2041 acgcgtgtgc ggcttcgggc tgtcgcaaag cgcgaagctg ttcaaagagt tcttgctccg
     2101 gtggcgatag acggtccggt aacattaccc ggatcctggc gtacaggtct ccttgaccgc
     2161 tgccgccgag tcggggcatc cccttaccgg tgagccggat cgtcttgccg ttctgcgagc
     2221 ccgccgggat cttcaacatc acccgtccgg tcatcgtcag caccggcgct tcaccgccga
     2281 gcaacgcgtc gtagagaggc acgtcgacgt cctcatatag gtcatcgccc ttgcgctcaa
     2341 aacgctcact tgggggcacg ttgatcagta gatacaagtc gcctttggaa ccgccgccgg
     2401 tgcctggctg accttcgccc gcgatgcgga cacgcgaccc gatcttgact ccggccggga
     2461 tttttacttc gagcttgcgc ggccgggtaa cgtagccgac gccatcacac acgcggcaga
     2521 tcgcttcgcc cgcgaggccc gttcctttac agcccacgca gggctcgttg ttctggatgg
     2581 tcagagtacg cgtggtgccc cgataggcct cttcgagcga aacgtcgagc ggatgctcga
     2641 ggttctcgcc tttgcgggca accggtttgc tgcgaccacg aaagacattg ccgaaaatgt
     2701 cgcccaggtc gcccaggtcg gtttcgacgc ctccgaagcc tccagtggtc cgtgtccccc
     2761 ggccagcggt acggaaccat tgtcccgcgc tcgcctgctg gcgctgttgc tcctctatct
     2821 ggtccgaata ttcccacttg tcgccgaatt tgtcgtattt acgacgcttc tctgcatccg
     2881 acaggacctc atttgcggcg ttgatttcct tgaattttgc ctcggcggtc ttgtcccctg
     2941 ggttcacgtc cggatggaat ttccgggcaa gcttgcgaaa ggcagcgcgg atctccttat
     3001 ttgtcgcatc ccgcttgacg cccaggatgt cgtaataatt catcgttggc atcgctgcct
     3061 cctgcccgaa tggccgttag acgccactcc gcggtagccg cattgagtat cgttgtaatg
     3121 atagtctgat aataacacac taaactctat tgacaataac gtttggagcc ctatccctcg
     3181 tcgaccagca ccacgcgcgc cggtgcaccg tcgccgcccg ccagctcggg aggtaggcag
     3241 agcaattgat atgcgctcct aacatccttg atatccgatt gcggctatac tgctgtcgca
     3301 actgggatgg agttgaggca tggtacaaga aaccacacgg agtagtgaac aaattcgcga
     3361 gcagtcccgg ttcctcaccg aagttcgatc ggaagtggcg aaggtcatcg tcggccagca
     3421 gtacctgatc agccgcctgt tgatcgcatt gcttaccggg aaccacgtcc tcatcgaggg
     3481 cgtccccggg ctcgcaaaaa cgctttccgt gaagacgctt tctgacgcgc tgcaccttgc
     3541 gttcgtgcgc gtccaattca cgcccgatat gctgcccgcg gaccttaccg gcaccacgat
     3601 ctacaacgcc cgtgacgccc agttcacggt gcggcaaggg ccgatcttcg cgaacatcgt
     3661 cctggcagac gagatcaatc gctcacccgc caaagtgcag tcggcgctcc tcgaagcgat
     3721 gcaggagcgc caggtcagta tcggcggcga aacttttccg ttgcccgcgc cgttcatggt
     3781 gctcgcaacg cagaacccga ttgaaagcga gggcacctat cccctcccgg aagcgcagtt
     3841 ggatcgattc atgatcaagg ctgtgatcgg ctatccgagc aggctggagg aacgtgcgat
     3901 cctcgaccgc ggtatctcgg gggagctggg cgaagtgcga cgaatcgtca gtcccgaaat
     3961 gatccttgcc gctcgggcga tggtgcgaga ggtttacctg gatgatcgcc tgaaggacta
     4021 tatcattcag atcatttcgg cgacccggaa cccgcgcgca ttccgcatgg atacgctggc
     4081 gccctttatc gcctatggcg cttcgccccg cgccacgatt ttcctcgcgc aggcggctca
     4141 ggcgcatgca ttcgtcgacg gccgcagcta cgtgacgccg gacgatatca aggagatagc
     4201 accggacgtt ttgcggcaca ggctggtggt aacctatgag gcggaggccg aagaggtctc
     4261 gcctgatgag atcgtcaagc gggtgctcgc cggtgtcgat gttccctgat ttggaatttg
     4321 cggcaatggg tgcggggtag ggtggtggca gagaccaacg aaccagtggt tcccggcggt
     4381 ggtctcacct ccgagttgtt gcgcaaggtg aagcggattg aaattcgctc gcgccggctc
     4441 gtcaacaacc tgtttgtggg tcagtaccac gcagtctttc gcggacgtgg gatagagttc
     4501 tccgaagtcc gcgaatactc gcccggcgac gaagttcgca cgatcgactg gaacgtcacg
     4561 gcacggctcg ggactccttt tatcaagaaa tttgttgagg agcgcgagct gagcgtcttg
     4621 ctgctggtgg acgtcagcgc gtcgggagcg tttgggacgg ttgcccaaac caagcgtgag
     4681 gtagcagccg aaatcgcagc gctgctggcg ctctccgcaa gcgacaacca ggaccgcgtc
     4741 ggcctgctcg catttaccaa tgagatcgaa aagtacatcg tgccggccaa ggggcagcga
     4801 cacgtgttgc ggctggtgcg cgagctcctg gcgcttcagc ccctgaatcg cggcacccgc
     4861 atcggcgcgg cggtggacta cgtttcccgg atcctcaaac gtcgctcggt cgtgttcatc
     4921 atttccgact tctgcgattc tggcgattac gagcgaccgt tgcgcgttct cggtcagaag
     4981 cacgatgtga tcgcggtggc gctcgccgat ccgcgagacg aagagttgcc caacatcggg
     5041 ctgcttgagc tcgaggacgc cgaaagcggt gagaccgtac tcgtcgacac gtcagacgcg
     5101 cgtgtgcgac gtcactaccg cgcgacggtc gaggcggcgc gcgcggagcg ccagcgtatt
     5161 ttccggacaa acagcatcga cgcgatagag atcaacacgg cggcgtcgta cgtcgagccg
     5221 gtcctggcat tcttccgccg gcgagagggc agcggcggaa ccggccgcgg ccggagggcg
     5281 ttgtcagcat gaatgcccgt gcatttcgcc tgttgctttt cattgtctgc ctgagtgccg
     5341 ggctggcgtc ggttcgtctg acgcagctat ctgcagcggc cgcgggcgag cagcaggaca
     5401 accagacgcc agacgtcacg gtcaacatcg atcgcgcgcg cgcgacggtt ggcgatccaa
     5461 tttcgctgac cgtgacgata aagcacctcg ccaacgtgac gatcgatacg acgtcgatcg
     5521 acgatcagct cggcagcctg gaaccgatcg cgtcggaccc gccagacgac cggcctgctg
     5581 gcggcggcat ggagttacgg ctgcgttaca agctcgccag ctaccacacc ggtacggtcg
     5641 agcttcccgt cttgacgttc acctataccc ttcccgacgg aacccaagcc caggtccagt
     5701 caaaagctcc ggttccaata acgatccaaa gcgtgctgcc cgacggtgca gacccgaccg
     5761 atatacgtcc cttgaagcag caattctcgt tgcccgtgcc ggcgtcagaa cagttgttgt
     5821 ggatcattgg cgggggtggc atcccggtcg cttttgtagt cgtcgcggtg ctgtcgtacc
     5881 tgttgatatg gcgcaagcgt ccgccgcagt cgatcgccgc gcgccacgcg acccaggcgg
     5941 cgcgcgctga cctggatcgc gtcatgggat tgggcctgtt agaacgcgga gagttgttgg
     6001 accactaccg gcagttggcg aactgtgtcc gccgctatct gaccgagcgg tacggtttcc
     6061 cggcagtggc gttgaccacc ggcgaattgg agcgacagat ggaggcgcgc gggatcgcgc
     6121 gttggcccgc ccggttgatc tccggcgtgt tgagcgagtg cgacgcggtc gtctacgcgc
     6181 gttatcagcc cgccgcagtc cgttccgaag ctgataatgc catggcatac gagatactag
     6241 acgcgactga cgacgccggc gttgcaccaa agccgatggt acaagcagtc tgagcggaat
     6301 caagggagag ataccgcgtg cccaacctac ctttccgact cgcagatccg gctttgctgg
     6361 ccttgctcct ggtggttcca tttagctggt tggcgttcca atggattgcg caccggcgaa
     6421 gctcaggggc gatgcgaatt tccacgatct taccgctacg tggtgtgccc gttgggtggc
     6481 gggtgcggta tcgctggacc ttgaacgcgc tccgattgct ggcggcaata cttgtcgtac
     6541 tcgcgctgtc gcgtcctcaa accggtaaag ccagcgctcg aatcccagct ca
//
LOCUS       DATCPE010000054         4819 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U7219
            k141_527729, whole genome shotgun sequence.
ACCESSION   DATCPE010000054 DATCPE010000000
VERSION     DATCPE010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955033
            Sequence Read Archive: SRR1586279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 4819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 16:49:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 640
            CDSs (total)                      :: 631
            Genes (coding)                    :: 627
            CDSs (with protein)               :: 627
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4819
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_527729"
                     /isolate="SMAG_U7219"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1022)
                     /gene="metK"
                     /locus_tag="VIM45_01680"
     CDS             complement(<1..1022)
                     /gene="metK"
                     /locus_tag="VIM45_01680"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEY8820029.1"
                     /translation="MCDQISDAVLDAILSADPKAHVACETACTTGLVVVMGEVTTDAW
                     VDVQRIVRDTVTRIGYTSDEYGFNADTCGVLVALHSQSPDIAGGVQESLEARQHVGEV
                     DELDLQGAGDQGMMIGFACDETPELMPLTIALAHRLTRRLTELRKNGTLPYLRPDGKS
                     QVTIEYAYGKPQRVDAVVISSQHDPSVSQEKIEKDVIAKVIRPVIEEFNSSLLDSDTK
                     YYVNPSGRFAIGGPMGDAGLTGRKIIVDTYGGIARHGGGAFSGKDPSKVDRSAAYAAR
                     HVAKNVVAAGLADRCEVQVSYAIGKAEPTSLAIETFGTANIDEDRILELIKDTFDLRP
                     AAIIADL"
     gene            complement(1176..2444)
                     /gene="ahcY"
                     /locus_tag="VIM45_01685"
     CDS             complement(1176..2444)
                     /gene="ahcY"
                     /locus_tag="VIM45_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008475508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEY8820030.1"
                     /translation="MTPQTRGDVRDRALANEGIRRIEWAAREMPVIALIRERFRKEQP
                     LTGVRVGACLHITTETANLLIALKEGGAEVTACASNPLSTQDDVAAALNVEYGITTFA
                     IKGEDHETYYRHINAVLDARPQLTMDDGCDLVATIHKERTDLIPGIIGGTEETTTGVV
                     RLHGMDKDGALKFPIVAVNDADTKHMFDNRYGTGQSTLDGVIRATNILLAGKNVVIAG
                     YGWCGRGVASRARGMGAQVIVTEVDAIRALEAVMDGFRVMSMMEASAVGDIFITLTGD
                     INVLDRPHLEAMKDGAILCNSGHFNDEINIGALESISEGQRPVREFVQEYRLGNGRKL
                     FLLGEGRLINLTAAEGHPASVMDMSFANQALSAEHIVINAGKLKPGVYGVPREIDEEI
                     ARLKLNAMGIGIDTLTEEQRHYLASWESGT"
     gene            complement(2532..3452)
                     /locus_tag="VIM45_01690"
     CDS             complement(2532..3452)
                     /locus_tag="VIM45_01690"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8820031.1"
                     /translation="MVKGARLSLAGHAHRHRYQVDGLFEPGRIRALLETRRLYSAYAL
                     GQLGSDLFPLVDVWRSAEETGGEGALALYSRGGLGDAMFTLGDPESLDAILSLHPGPR
                     QNYASCEPRHLDTMRRYFRFSLERPMLRMGVSLRTFRRPAEPSGHLYIRRLLATDVRL
                     LNRLYNSEGSPTYYSGQVIETGCYFGVYEDGRLVSVAGTHVVTPEEGIAVVGNVFTHP
                     NWRSRGLAQIATGATTSFLLQRCRDVVLTVDPGNAPAVRAYERLGYSTDCRLIEAAVT
                     RRELIGAGSYVRRLAARIRGRQEGTEIVIR"
     gene            complement(3454..4362)
                     /locus_tag="VIM45_01695"
     CDS             complement(3454..4362)
                     /locus_tag="VIM45_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011734379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEY8820032.1"
                     /translation="MSILVVGSAAFDDIETPFGRAEHILGGSTIYFAAAASLFAPVNV
                     VSVVGTDMPLDAYDFLKARGVDMRGLQVEDGKTFHWSGRYDYDLNQTETLVTDLNVYA
                     DFRPSLPDGYADSPFVFLANIQPDLQRSVLEQARSPRLTMMDSMNLWIETQRDAVEAI
                     MRMVDMVSINETEARLLMRTSSVLAAAKRILTLGPKAVLVKRGEYGAVLFTEDDYFAA
                     PGYPLEEVYDPTGAGDSFAGGFMGYLAQAPEINGATLRQAVIHGSAVASFTVQAFGVE
                     RLKTLTRSEVEDRCREFQRFTSFGEV"
     gene            complement(4409..>4819)
                     /locus_tag="VIM45_01700"
     CDS             complement(4409..>4819)
                     /locus_tag="VIM45_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010936294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HEY8820033.1"
                     /translation="LVVMEGPAFSTRAESELYRTWGASLIGMTALPEAKLAREAEICY
                     AAVACATDYDCWHETEADVTADLILANLLKNVEVSKRIVRDAVTRLTADRTCPCANAL
                     QTAIITQPGRVPRQVKRDLAPIIGRYLRMTVSAT"
BASE COUNT          860 a         1523 c         1476 g          960 t
ORIGIN      
        1 aggtcggcga taatcgccgc cggccgcagg tcgaaggtgt ccttgatcaa ctccagaatg
       61 cgatcttcgt caatgtttgc ggtgccgaac gtctcgatcg ccagcgacgt gggctcagcc
      121 ttgccgatgg cgtacgaaac ctgcacttcg cagcggtcgg ctaaaccggc cgcgacgacg
      181 ttcttcgcca cgtgtcgtgc cgcgtatgcc gccgaacggt cgactttgga cgggtctttg
      241 ccggagaacg ctccgccgcc gtgccgcgcg atgccgccat acgtatcgac aatgatcttg
      301 cgacccgtca gccccgcgtc gcccatcggc ccgccgatcg cgaagcgccc cgacgggttg
      361 acgtaatatt ttgtgtctga atcgagcaac gacgaattga attcctcgat caccggacgg
      421 attaccttcg cgattacgtc cttctcgatc ttttcctgac tcacagacgg gtcgtgctgg
      481 ctcgagatca cgaccgcgtc tacgcgttgc ggcttgccgt aggcgtattc gatcgtcacc
      541 tgtgacttgc cgtccggtcg cagatacggc agggtgccgt ttttgcgcag ctccgtgagc
      601 cggcgggtca acctgtgcgc taaggcgatg gtgagcggca tcagctcggg ggtctcgtcg
      661 caggcgaagc cgatcatcat gccctggtcg cctgcgccct ggaggtcgag ctcgtcaact
      721 tcaccaacgt gctgtcgggc ctcgagcgac tcctgcacac cacccgcgat atcgggtgac
      781 tggctgtgca gggcgaccag cacgccgcac gtatcggcgt tgaagccgta ctcgtcgctc
      841 gtgtagccga tccgcgtcac cgtgtcgcgc acaatgcgct gcacgtctac ccaggcgtcg
      901 gtggtgacct ctcccatgac caccaccagg ccggtggtgc aggcggtctc gcaggcaacg
      961 tgcgccttgg gatcggctga gaggattgcg tccagaacag catccgagat ctggtcgcac
     1021 atcttgtcgg gatgaccttc agtcaccgat tccgacgtca ggaatagcga tcgcgagttg
     1081 ctgaacgttg tgcccactaa gattgtcctc ctgttgttgc ggcgccatat caggcgctga
     1141 tttctattga cggcttggag tgcagcaata tgctcctaag tgcctgactc ccatgaggcc
     1201 aggtaatgac gttgttcctc ggtcagcgtg tcgatgccga tccccatcgc gttcagcttc
     1261 agccgggcaa tctcctcgtc gatctcccgc ggcacaccgt aaacgcccgg cttgagcttg
     1321 ccggcgttga tgacgatgtg ctcggcggaa agcgcctggt tggcgaagct catgtccatg
     1381 acgctcgccg ggtggccttc ggccgccgtc aggttgatca gccggccctc gccaagcaag
     1441 aagagcttac gaccgttgcc cagcctgtac tcttgaacga actctcgcac gggtcgctga
     1501 ccctcgctga tcgactcgag ggcgccgatg ttgatttcgt cgttaaagtg gcccgagttg
     1561 cacaggatgg cgccgtcttt catcgcttcc aggtgcggcc ggtccaggac gttgatgtcg
     1621 ccggtcaacg taatgaagat atcgccgacc gccgaagcct ccatcatgga catcacgcgg
     1681 aagccgtcca tcacggcctc gagggcgcga atggcgtcga cttcggtaac gatgacctgt
     1741 gcacccatgc cgcgtgcgcg gctcgcgacg ccgcgaccgc accagccata cccggcgatc
     1801 accacgttct taccggcgag caggatgttg gtggcgcgga tgacgccgtc cagcgtgctc
     1861 tgacccgtgc cgtagcggtt gtcaaacatg tgcttggtgt cggcgtcgtt gacagccacg
     1921 attgggaact tgagcgcgcc gtccttgtcc atgccgtgca gccgcacgac gccggtcgtg
     1981 gtctcttcgg taccgccgat tattcccgga atgaggtcgg ttcgctcctt gtggatcgtc
     2041 gcgacgaggt cgcagccatc gtccatcgtt agctgcggcc gcgcgtccag caccgcgttg
     2101 atgtgccggt agtaggtttc gtggtcctcg cccttgatcg cgaacgtcgt gatgccgtac
     2161 tcgacgttaa gcgccgcggc gacgtcgtcc tgggtcgaaa gcgggttgct ggcgcaggcg
     2221 gtgacctcgg ctcccccctc tttgagcgcg atcaagaggt ttgctgtctc ggtcgtgata
     2281 tgcaggcagg cgccgacccg cacaccggtg agtggctgct ccttgcgaaa acgttcgcgg
     2341 atgagggcga tgacgggcat ctcgcgcgcg gcccactcga tgcggcgaat gccttcgttg
     2401 gcgagcgccc gatcccggac atcgccgcga gtctgaggcg tcatggtccc tgtggtcaag
     2461 ttccttgctc ctttttgaac cgtactgttg aatcgttctc cgacggggtc gaaatgaggg
     2521 caaaagcacc ctcaccgaat cacgatctct gtcccttcct ggcggccgcg gattcgggcg
     2581 gccaaccgcc tgacatagct gccggccccg atcagttctc ggcgggtcac ggccgcttcg
     2641 atcaaccggc agtcggtgct gtagcccagc cgctcgtacg cgcgcaccgc cggtgcattg
     2701 ccaggatcga ctgtcaaaac gacgtcgcga cagcgctgca gcagaaaact agtcgtcgct
     2761 ccggtggcga tctgggcgag accgcggctc cgccagttcg gatgggtgaa cacgttgccg
     2821 accacggcga tgccctcttc gggggtcacg acgtgggtgc cggctacgga gacaagccgg
     2881 ccgtcctcgt acacgccgaa gtagcaccct gtttcgatga cctggccgct gtagtaggtc
     2941 ggggagcctt cgctgttata gagccggttc agcagccgca cgtcggtcgc gagcaggcga
     3001 cgaatataca agtgcccgct cggttcagcg ggacggcgga acgtccggag gctcacaccc
     3061 atgcgaagca tcggccgctc taacgagaac cgaaagtagc gtcgcatcgt gtcgaggtgt
     3121 cggggctcgc agcttgcata attctgcctc ggtcccggat ggagcgagag aatcgcgtcg
     3181 agcgactcgg gatcacccag cgtgaacatg gcgtcgccca ggcctccccg cgagtagagc
     3241 gccagtgcgc cctcccctcc ggtttcctcc gctgaacgcc agacgtccac caggggaaag
     3301 agatcagaac ccagctggcc gagagcatag gcggagtaga ggcgccgggt ttcgagcagc
     3361 gcgcggatgc gcccgggctc aaatagcccg tccacctggt accggtgacg gtgggcatga
     3421 cccgcaaggg acagtcgcgc tcccttgacc acgctagacc tctccaaaac tggtgaatcg
     3481 ctggaactcc cggcagcggt cttcgacctc tgaccgcgtt aacgtcttca atcgctcgac
     3541 gccaaacgcc tgcacggtga aactggcgac ggcactgccg tgaatgacgg cttgcctgag
     3601 cgtggcaccg ttgatttccg gcgcctgcgc gagatatccc ataaaaccac cagcaaagct
     3661 atcgccggcc ccggtcggat cgtaaacctc ctcgagtgga tacccgggtg ccgcaaagta
     3721 gtcgtcttca gtaaatagga cggcgccata ttctccgcgt ttgacgagca cggccttggg
     3781 tccgagcgtc aggattctct ttgcagcagc cagcacgctg ctggtccgca tgagcaggcg
     3841 ggcctccgtt tcgttgatgc tgaccatgtc taccattcgc attatcgctt cgactgcgtc
     3901 cctctgcgtc tcaatccaca gattcatcga gtccatcatc gtcaatcggg gcgagcgcgc
     3961 ctgttcgagc actgaacgct gcaaatccgg ttgaatattg gccaggaaga caaaagggct
     4021 atcggcgtat ccatccggaa gcgatggccg aaaatcggcg tagacgttca ggtcggtgac
     4081 tagcgtctcc gtctggttga ggtcgtagtc gtagcgccct gaccagtgga acgtcttgcc
     4141 gtcctctacc tgcaggccgc gcatgtctac acctcgcgcc ttgagaaagt cgtaggcgtc
     4201 aagcggcatg tcggtgccga ccacggagac tacgtttacc ggcgcgaaca gactggctgc
     4261 ggccgcaaag taaattgtgg agccgccaag gatatgctcg gcacggccaa acggcgtttc
     4321 gatgtcgtcg aaggctgcgc tgccaaccac caggatgctc aaaccagaac ccctcttttc
     4381 aaactggatg gaatagtccg tggcaccctc acgtagcaga aaccgtcatt cgaagatagc
     4441 gcccgataat cggcgctaga tcgcgcttga cctgtcgcgg cacacggcca ggctgagtga
     4501 tgatcgccgt ctgcagggcg ttggcgcacg gacaggtccg atcggccgtc aacctggtta
     4561 cggcgtcacg cacgatgcgc tttgagacct ccacgttctt caacaggttg gccaggatca
     4621 ggtcggcggt cacgtcggct tcggtttcat gccaacaatc gtagtcagtt gcgcaggcga
     4681 ccgccgcgta acagatctcg gcttcgcggg ccagcttcgc ctcaggcagc gccgtcatgc
     4741 cgatcaggct ggccccccac gtccggtaca gctcagattc ggcgcgggtg gaaaaggcgg
     4801 gtccctccat gactaccag
//
LOCUS       DATCPE010000055        12359 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U7219
            k141_41255, whole genome shotgun sequence.
ACCESSION   DATCPE010000055 DATCPE010000000
VERSION     DATCPE010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955033
            Sequence Read Archive: SRR1586279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 12359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 16:49:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 640
            CDSs (total)                      :: 631
            Genes (coding)                    :: 627
            CDSs (with protein)               :: 627
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12359
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41255"
                     /isolate="SMAG_U7219"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(82..960)
                     /locus_tag="VIM45_01705"
     CDS             complement(82..960)
                     /locus_tag="VIM45_01705"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8820034.1"
                     /translation="MQQASPLAGDVAWLVAVRQRRAVARNVAFIEPAAGSVRIRSLSR
                     AVFRSVARYYVELLRLPYTEIDELHGRIDVTGWENFAAANERGAGVIVASLHYGPAEI
                     VLQAFGGRGIRYTAMIERIRPIPLAKLFLCLREAHGNQYVFPGRAGARQLIRALKSGG
                     VVALMVDRDVTGGGMHVTFCGGTVSVPAGPVELAHLTGAAIIPAIGRWRPHNRYEVTL
                     LPAVIPEPDRRKPKMLRVEIEAVLAELEPYLRREPEQWIVLLPLWNERMPGVSPAYTG
                     VTDRGPRSTGGAVGDS"
     gene            complement(1057..1950)
                     /locus_tag="VIM45_01710"
     CDS             complement(1057..1950)
                     /locus_tag="VIM45_01710"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8820035.1"
                     /translation="MIYWLWRLGLSLVRFVPLRVSYAVAGPVADVAWLFMPVQRRNAV
                     ENMTQVVGSRSNPKAKSLARRSFRNYAKYAIDFMRHPEINPDDLMARLDFDDWHEFDA
                     ALANGRGIILVLMHFGNWDLGGAALTAHGYTLNVIAETQAHSRFNDAIVAARLTRGMK
                     LIPMERAATGIVRAMRRNEMLAILIDRPLTEGGVAVQFFGAETRVPEGPARIALRTGA
                     RVFPVSLLAVADRVRAIIDFDVRQVHTGNMERDVQSLTQQIMTSHERVIRQHPDQWYM
                     FRRMWPAGARVETPPAVTAES"
     gene            complement(1989..3089)
                     /locus_tag="VIM45_01715"
     CDS             complement(1989..3089)
                     /locus_tag="VIM45_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018287644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HEY8820036.1"
                     /translation="MGKINVAIVGVGNCASSLVQGIEYYKNAKEDEFVPGLMHVNLGG
                     YHIRDCRVVAAFDIDADKVGKDLSEAIFSGQNNTFKFCPVPKTGVIVHRGMTHDGLGK
                     YLSQVITKAPGPTADIVNILKETKTDVVINYLPVGSEEATRWYVEQVLQAGCGMINCI
                     PVFIAREPYWRKRFEDVGVPIIGDDIKSQVGATITHRVLTRLFEDRGVRIDRTYQLNF
                     GGNTDFLNMLERERLESKKISKTNAVTSQLTNSPLDPDNIHVGPSDYVPWLQDRKWCH
                     IRIEGTTFGDVPLNLELKLEVWDSPNSAGVVIDAIRCVKLALDRGMSGALFAPSSYFM
                     KSPPQQYHDDDARRQLEQFIAGSQPDHALEQE"
     gene            complement(3301..4614)
                     /gene="rimO"
                     /locus_tag="VIM45_01720"
     CDS             complement(3301..4614)
                     /gene="rimO"
                     /locus_tag="VIM45_01720"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015940366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HEY8820037.1"
                     /translation="MRYHLVTLGCPKNTVDSEQLERLLLASKHTPTVRPSDADLLIVN
                     TCGFIDASKEESVNTILRLAALKKPGQKLVAAGCMTQLYGDDVASEIPEVDHIFGVNQ
                     WESVAALAAGERNAPWDIPVTSSPMVARTSAYLKISDGCNAPCTFCIIPQIKGRFTSA
                     SAGQVVGEARRLVAEGARELVLVAQDSTAWGEDIGLRDGLADLLHVLADSLPERPWLR
                     IMYAYPGRVSPRLIDAMATIPNVVHYLDMPLQHASVKMLKRMRRPANMEMVRRTLADL
                     RSAMPDIALRTSLIVGFPGETESEFSELKDFVGEVRFDHAGVFTYSPQDGTPSATMDA
                     QVPERTKRRRRAELMEILQTISNERNRSLIGSELTVLIEASSQERGGSGRVVSVGRSY
                     RDAPEVDGVVIVRGEFAPGTLLPVRVTGAMPYDLVADPIAALTPA"
     gene            complement(4629..5522)
                     /locus_tag="VIM45_01725"
     CDS             complement(4629..5522)
                     /locus_tag="VIM45_01725"
                     /inference="COORDINATES: protein motif:HMM:NF015734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY8820038.1"
                     /translation="MAGLGESLQNTRHMRGVSLEEAERATHISRRYLQALEDEDFSVF
                     VAPVFARGFLRNYSQYLGLDPEEMLSYWPQSPAAAAAAPEPESTRDDIEYRRGRVQDR
                     TPPRRVAVTRGTEPPSPLSRPNLAPAEHTPGSRVLAVAAGLVVCMGVIAFVAGKAAGR
                     SASSPVRSGGAAAPTPGGPRRTSGAAQPRLTPTPPSRSAGAMPALLGQDAQSAIQQLQ
                     QRGVTPLVVSVTSTNRADRPGTVIRQEPSAGTGITTNSGVTLVVDQAAPAPGAPAIST
                     PPRVPAFGTPSGTRTATAPAR"
     gene            complement(5564..5839)
                     /gene="rpsT"
                     /locus_tag="VIM45_01730"
     CDS             complement(5564..5839)
                     /gene="rpsT"
                     /locus_tag="VIM45_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEY8820039.1"
                     /translation="MPNIASARKRMRQAGKRRLRNRIVISTTRTYIHRAERELSLGES
                     GSAASTIHLAIRALDKAAEKGIIHKNNAARRKSRLMHKLNAATANSA"
     gene            complement(5953..6645)
                     /locus_tag="VIM45_01735"
     CDS             complement(5953..6645)
                     /locus_tag="VIM45_01735"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEY8820040.1"
                     /translation="MQTGETARALSCVPLFANLPSGVIDAIGARVRRRRYSSGEVIFH
                     EGDPGRSLCLIDSGHVKIVSVSEQGQEAMLAVMGTGEFFGELALFDDVPHSADVVAME
                     TTQTLNLAREDFFAIIDHYPAVSRQIFSLLASQIRRLTNGVGDMIFLNLDARIAKRLL
                     ELAHSHGVDVPEGRRIELSLSQAELGSMVGATRESVNQCLRRFQHAGLILLGRQRITV
                     VKAAALRRRIIY"
     gene            6907..8016
                     /locus_tag="VIM45_01740"
     CDS             6907..8016
                     /locus_tag="VIM45_01740"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8820041.1"
                     /translation="MVIVHTFFLSNFLLTAALPLYVAKSPRWQVGLVVGVPFIASLIL
                     RPFTGRLVDRSGRRVYLITGAAGCAAMMALHAVSANIWFLAGVRLLLGAASAFITTAI
                     MASLADVLPPTRRGAGMGWYGIFYTSTNLYGPALGLWLASSFGYAFMFGFDAALNVAA
                     LLVALALTETGVRTIKGTPPARLFSRSALLPMSTFLSITIPAGAVTAFLALVEKQRHG
                     GDPGLFFLLFGVTLMSGRVIGGWVSDRFSRSAAIVPGLVLTGVAMLGLAAAGAPVAFY
                     LVALMYGFGFALGHTGSTILTMDRAPEAERGAAMATLTVAWDIGTLVGAFMLGFLVDA
                     AGYGVVFLLVGLIPIAQAGIYMVIAKPRRSVVAPS"
     gene            complement(8073..8459)
                     /locus_tag="VIM45_01745"
     CDS             complement(8073..8459)
                     /locus_tag="VIM45_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HEY8820042.1"
                     /translation="MYERRSVSRRRFESLVRRALKDLPAEFAGRLENIDIVVRARAND
                     DQLHAGKTQPRQTLLGLYVGTDLTRRGYGYNFALPDRIFIFQQPLERIARDEEHLTEL
                     VRHTVLHELAHHFGIDDDRLHEIGRY"
     gene            complement(8713..9708)
                     /gene="tsaD"
                     /locus_tag="VIM45_01750"
     CDS             complement(8713..9708)
                     /gene="tsaD"
                     /locus_tag="VIM45_01750"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015407239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEY8820043.1"
                     /translation="MRILSVETSCDESAAAVIEDGHRILSNMVATQIDVHRRYGGVVP
                     EVASRQHLQTIIPVIETAMHSSGCNWDDIDGIAVTHGPGLAGSLLVGVNAAKAVAFAR
                     RKPLVGLNHLESHIYANWLDTETPTEPPEFPALCLVVSGGHTDLIIMCGHGEYKRLGR
                     TVDDAAGEAFDKVARLLELGFPGGPAIEWTAATIVHTDVVLPRAWLPGTWNFSFSGLK
                     TAVLHMLREPDHPPVADIAKAFQESVVDVLAAKTVRAAREFGVKQILVAGGVAANGAL
                     RKELTARAPVQVRVPPPKYCTDNAAMVGACGYFHFSAGDAKDFDLDVEPNLAFAS"
     gene            complement(9742..10431)
                     /locus_tag="VIM45_01755"
     CDS             complement(9742..10431)
                     /locus_tag="VIM45_01755"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY8820044.1"
                     /translation="MTGIPVRLMVVDDHRLFRDGLASLLNSYDDVELVAEAGSGPEAI
                     EKAKLFKPNVVLMDMSMPGMPGTEATKTILELAPETAVCMLTVSEQDEDLFAAIRSGA
                     RGYLLKDTELRALHDALQVLADGGTSITPVLATRLLDEFAKASPPPRAAGPGLDQLTS
                     REREILEYVAAGWRNQEIADHLYIAVNTVKVHLRNILEKLDLRNRQQAAAFAAQEGLL
                     GQTQPARRNDA"
     gene            complement(10473..11303)
                     /locus_tag="VIM45_01760"
     CDS             complement(10473..11303)
                     /locus_tag="VIM45_01760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEY8820045.1"
                     /translation="MGRSLRAYMGDRASSYAAAIAYYTLFSVLPLSIFVIGFGSYFVT
                     NSDRDRMVANIADALGGASTVKIYQQVQSFTKGRAELGVIGLIGALWGASAVFTAVRT
                     GLNVVWNTSDGRPWLIAKAVDLLSVIGFGLLLALALAGVAGLVALSELTKEMLGSRVA
                     GLTSFGLGVFFLVLPFLIAFATFSQLYVVTSPASVHWTDCWHGAAVAAAGFQATSYGF
                     GWYVRSFGRFDKLYGSLGAVIAFVFYAYVVASLVLLGAEIARQYVEWKRRRPSCVPVL
                     "
     gene            11604..>12359
                     /locus_tag="VIM45_01765"
     CDS             11604..>12359
                     /locus_tag="VIM45_01765"
                     /inference="COORDINATES: protein motif:HMM:NF012879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEY8820046.1"
                     /translation="MPTWQIATLKNGMRIVTTPIPTAQSASVNVFVGVGSRAEERRVN
                     GVSHFMEHMLFKGTNRRPNAIIIASEIEGAGGVLNAYTTKELTCYWNQVPYDMLPLAM
                     DVVADMVRNSLLEDVEVERERTVVQQEIRRAHDQPGAWVGELLSGAVYGDQPIGWSIA
                     GTLDIVAGISRDDMRRHLDTWYVPSNLVLSVAGNVIHQQVVELSQKLWEDMPGDVTPS
                     VTPAVPEMSPDRIRMDQRDITQANMAIGLRSLHR"
BASE COUNT         2229 a         3825 c         3903 g         2402 t
ORIGIN      
        1 agggacgtct ggtgctccgc gtactccaac acctcttccg gactggccat gccgtcgctg
       61 cccgtcgtgt ggatatgcag gtcagctgtc cccaacggct cccccggtgc tgcgcggacc
      121 gcgatcagtc accccagtat acgccggact cacgccgggc atccgttcgt tccacaacgg
      181 cagcaagaca atccattgct caggttcgcg gcgcagatat ggctccaatt cagccagaac
      241 agcctcgatt tccacacgca acatctttgg tttcctgcgg tctggttccg gtatgacggc
      301 gggtaacagc gtcacctcat agcgattgtg tggtcgccag cgcccgatcg cgggaataat
      361 ggcggcgccg gtaaggtgcg caagttccac tggtccggca ggtacgctca cggttccacc
      421 gcagaaggtg acatgcatac cgccgccggt tacgtcgcgg tcgaccatga gggcgacaac
      481 gccgcccgac ttcagcgccc ggatcagttg tcgtgcgccc gcgcgaccgg ggaaaacgta
      541 ctgattgccg tgggcttcgc gtaggcacaa gaacagcttc gccagtggga tcgggcgaat
      601 gcgttcgatc atcgccgtgt aacgaattcc acggccgcca aacgcctgga gcacgatctc
      661 ggcgggacca tagtgcagac tggcaacaat gacgccggca ccccgttcat tggcggccgc
      721 gaagttttcc caccccgtta cgtcgatgcg accgtggagc tcgtcaatct ctgtgtaggg
      781 cagccggagt agctcgacat aatacctggc caccgagcgg aagacggcgc gtgacagcga
      841 ccgtatccgc acagatccgg cggccggctc gatgaaggcg acgttgcggg cgaccgcgcg
      901 ccgctgcctc accgcaacca gccatgccac gtcgccggcc agcggacttg cctgctgcac
      961 taggctgggg gccacacgca ccgctgcgga ggcgacctgg aacatccgcc acttgagaac
     1021 gggtataagc ccaagatgcc cgcgatgcga catggttcag gattctgcag tcactgccgg
     1081 cggcgtctcc acccttgcgc ccgcgggcca catccggcgg aacatatacc actggtcggg
     1141 gtgctgacga atgacgcgtt cgtgcgaggt cattatctgc tgcgtcagcg actgcacgtc
     1201 ccgctccatg ttgccggtgt gtacctgcct cacgtcgaaa tcgataatcg cccttacgcg
     1261 gtcggcaacg gccagcaggc tgaccgggaa aacccgcgct ccagtacgga gggcgatgcg
     1321 cgccggacct tccggcacgc gcgtctccgc gccgaaaaac tgcaccgcta cccctccctc
     1381 ggtcaatggc cggtcgataa ggatggcgag catctcgttg cggcgcatgg ctcggacgat
     1441 gccggtggcc gcccgttcca tgggaatcag cttcatgccg cgggtgagcc gagctgcaac
     1501 gatcgcatcg ttaaagcggc tgtgcgcctg cgtctcggcg atcacgttca gcgtgtagcc
     1561 gtgtgcggtg agtgcggcgc cgccgaggtc ccagttgcca aaatgcatca gcacaaggat
     1621 gatgccgcga ccgttcgcca gagctgcatc gaactcgtgc cagtcatcga agtcgagcct
     1681 ggccatcagg tcgtccggat tgatttccgg gtggcgcata aaatcgatgg cgtacttggc
     1741 gtagttgcga aacgatctgc gggcgagcga tttggccttt gggttcgacc ggctgccgac
     1801 tacctgcgtc atgttctcga cggcgttgcg acgctggaca ggcatgaata gccacgccac
     1861 gtccgcgacg ggccccgcca cggcatacga gacgcgcagc ggcacgaatc tcacgagcga
     1921 taggcccaac cgccagagcc aatagatcat ggggtacgat cagcggcgcc gggtcgcagc
     1981 acgtccggct attcctgctc gagcgcgtgg tcgggctgac tgcccgcgat gaactgttcg
     2041 agctggcggc gcgcgtcgtc gtcgtggtac tgctgcggcg gcgacttcat gaagtacgac
     2101 gatggggcaa agagggcgcc cgacatgccc cggtcgagtg cgagcttaac gcagcggatc
     2161 gcgtcgatca cgacgccggc cgagttcggg ctatcccata cttcgagctt cagttccagg
     2221 tttagcggta cgtcgccgaa ggtcgtccct tcgatgcgga tgtggcacca cttacggtcc
     2281 tgcagccatg ggacgtagtc gctggggccg acatggatgt tatcgggatc gagcgggctg
     2341 ttcgtgagtt gtgacgtgac cgcgttggtc ttggagatct ttttcgactc aagccgctcg
     2401 cgctcgagca tgtttaagaa atcggtgttg ccgccgaagt tcaactggta ggtacggtcg
     2461 atgcgcactc cacggtcttc gaataagcgc gtcaaaacgc gatgcgtaat cgtggcgcca
     2521 acctgcgact tgatgtcatc gccgatgatc ggcacgccga cgtcctcaaa ccgcttgcgc
     2581 cagtagggtt cgcgggcgat aaacacgggg atgcagttga tcatcccgca gcccgcctgg
     2641 agcacctgtt cgacgtacca ccgggtcgct tcttcgctgc cgacgggcag gtagttgatc
     2701 acgacgtcgg tcttggtttc tttgaggatg ttgacgatgt cggccgtcgg ccccggcgcc
     2761 tttgtgatta cctgcgacag gtacttgccg aggccgtcgt gggtcatccc gcggtgaacg
     2821 ataacgccgg ttttcggcac cgggcagaat ttaaacgtgt tgttctgccc ggagaaaatg
     2881 gcttctgaca ggtctttgcc aactttgtcg gcgtcgatgt cgaaagcagc cacgacccgg
     2941 cagtcacgga tgtggtagcc gcccaggttg acgtgcatca aacccgggac gaattcatcc
     3001 tctttcgcgt tcttgtagta ttcgatcccc tgaaccaggg aggacgcgca gtttcccacg
     3061 ccaacgatgg ctacgttgat tttgcccaag atgccggttg ctcctcgccc atgtccgccg
     3121 cgaaaacgca ctgcgaaccg tggccacggc tgggcgaacc cgctgtttta tgccacatgg
     3181 ttcaggcccc gccagcggac gaggcccaac gcggattgta acagcgcccg aaacgtccga
     3241 gcaagttacg ggcggtcata aaatctcgga accgagcgat tatagcgtca cgcctcgaac
     3301 tcaggcgggc gtgagtgcgg cgatgggatc tgccaccagg tcgtacggca tcgcgccggt
     3361 cacgcgcacc ggcaacaacg tacccggtgc gaactcgcca cgcacaatca ctactccgtc
     3421 cacttcgggt gcgtcgcggt agctgcgacc gacagagacc actcgaccgc tcccgcctct
     3481 ctcctgtgag ctcgcctcaa tcaagacggt taactcgctt ccgatcaacg accggtttcg
     3541 ctcgttcgag atagtctgca gtatctccat gagttcggcg cgccgccggc gcttggtcct
     3601 ctccggcacc tgggcatcca tggttgccga cggagtccca tcctgtggcg aatacgtgaa
     3661 gacgcccgca tggtcgaagc ggacttcgcc gacgaagtcc tttagttctg agaactcgga
     3721 ctccgtttcg cccggaaagc caacgatcaa cgaggtgcgg agggcgatgt ccggcatcgc
     3781 cgagcgcagg tccgcgagtg tgcgccgcac catctccatg tttgcggggc gccgcatacg
     3841 cttcaacatc ttcacgctcg cgtgctgcag cggcatgtcg aggtagtgga caacgtttgg
     3901 gatcgtcgcc attgcgtcaa tgagccgggg agatacacgg ccggggtagg cgtacatgat
     3961 gcggagccac ggacgctccg gcagcgaatc agccagtacg tggagcagat ctgccagccc
     4021 atcccgcagc ccgatgtcct cgccccaggc ggtgctatcc tgtgcgacca acacgagttc
     4081 ccgcgcgcct tcggcgacca gccgccgcgc ctcaccgacg acctgtcccg cgctggcgct
     4141 ggtaaagcgg cccttaatct gcgggatgat gcagaacgtg cacggcgcat tacagccatc
     4201 cgagatcttg aggtaggcgc tggtacgcgc caccattggc gacgacgtaa ctgggatatc
     4261 ccacggggcg ttgcgctccc cggcggcgag cgcggccacg ctctcccatt ggttgactcc
     4321 gaatatgtga tcgacctcgg gaatttcgct tgccacgtcg tcgccgtaga gctgcgtcat
     4381 acagcccgcg gccacgagct tttgtccggg tttcttgagc gctgccagtc gcaggatcgt
     4441 gttgaccgat tcctctttcg aggcgtcgat gaagccgcac gtattgacga tcaacaggtc
     4501 tgcatcggaa ggccgcaccg ttggcgtgtg cttactggcg agcaggagcc gttccagttg
     4561 ctctgaatcg accgtatttt tggggcaacc gagtgtgaca aggtgatacc gcatgtgatt
     4621 cctaattact atcgggctgg agccgtagcc gtgcgcgtac cagatggtgt gccaaaggcg
     4681 ggaacccgcg gcggcgtcga gatagctggg gcgcccgggg ccggcgcagc ctgatccacc
     4741 accaacgtaa cgccggagtt ggtggtgatg cccgtgccgg ccgacggctc ctggcgaatg
     4801 acggtgccgg ggcggtcggc acggttcgtg ctggtcaccg acacgactag cggcgttacg
     4861 ccgcgctgct gcaattgctg aatcgccgac tgggcatcct ggcccaggag agccggcatc
     4921 gctccggcgc tgcgcgaggg tggggtgggt gtcagtctgg gttgggcggc accggatgtt
     4981 ctgcgtgggc cacctggcgt tggggcagcg gcgcctccgc tgcgaaccgg actgctggcg
     5041 ctacgtccgg ctgcctttcc ggcgacaaaa gcaatcaccc ccatgcatac cacgaggccg
     5101 gcggcgaccg ctagcacacg cgaaccgggg gtgtgttcgg ccggagccag gttgggtcgg
     5161 gacagcggac tgggtggctc ggtgccacgg gtaaccgcaa cccggcgtgg aggtgtgcga
     5221 tcttggaccc gtccgcgacg gtattcaatg tcatcgcggg tgctttccgg ctctggagca
     5281 gccgccgcag cagccggtga ttgcggccag tacgacagca tctcttcggg gtcaagcccg
     5341 agatactggc tgtagttacg gagaaatccc cgcgcgaaaa cgggagcgac aaagacggaa
     5401 aaatcttcgt cctcgagggc ctggaggtaa cgtcgcgaga tatgcgttgc tcgctcggcc
     5461 tcctccagag agacgccgcg catgtggcgc gtattctgga ggctctctcc gaggcccgcc
     5521 atccgtcctc cggtgtgaag ggtggtagac gtgccctcga gtgtcaggcg ctattcgccg
     5581 tcgcggcatt gagtttgtgc atcaaccggg atttgcgacg cgctgcgttg ttcttgtgga
     5641 tgatgccctt ctcggccgcc ttatcgaggg cgcggatcgc aaggtgaatc gtcgaagctg
     5701 cagaacccga ttcgcccagt gacagttcac gttcggcgcg gtgaatgtac gtcctggtgg
     5761 tgctaatcac gatacggtta cgcaggcgtc gcttgcctgc ctgccgcatc cgctttcggg
     5821 cggaggcaat attcggcaac ttgggaaccc ctctgttggt gagctaatcc accctatgat
     5881 acggaagtaa gcaaactgtg tccagaacgg tcacgcggcg cacgcgctcg accgcgacct
     5941 ttcgcacgtg cgtcaataga tgatccgccg tctcaacgca gccgccttga cgacggtgat
     6001 ccgttgccgt cccagcagga tgaggccggc atgctggaat cgtcgcagac actggttgac
     6061 gctttcccga gttgcaccca ccatcgatcc tagctctgcc tggctgagcg acagttcgat
     6121 tcgccgtcct tcgggtacgt caaccccgtg agaatgagcc aattcaagca agcgcttcgc
     6181 gatacgagcg tcgaggttca ggaagatcat gtcgccgact ccgttggtca gccgcctgat
     6241 ctgcgaggcc agcaaactga agatctggcg ggatacggcg gggtagtgat cgatgatcgc
     6301 gaaaaagtcc tcacgtgcga gattgagcgt ctgcgtcgtc tccatcgcca ctacgtcggc
     6361 cgaatgcggc acgtcgtcga acagcgccag ctcgccgaaa aactcacccg tgcccatcac
     6421 cgccagcatt gcttcctggc cctgctcaga gaccgaaacg atctttacgt ggcccgaatc
     6481 gatcaggcaa agcgatcgtc ccgggtcgcc ctcgtgaaag atcacttcgc cagagctgta
     6541 gcgccgccgc cgcacccggg cgccgatggc atcaatcacc cccgaaggca ggtttgcgaa
     6601 caggggaaca cacgagagtg cgcgggcggt ctcaccggtt tgcaccgcga aagtgctcct
     6661 cgacatgtgg agatgattac ggatgacaga atgccgcggg ctcactctac actgccggct
     6721 gtgcccgatg ccactgttgg cgccgcgcgg ccagcgcgtt gacgcgcgtt caacgcgaca
     6781 tctagcattc ttttgatgac ctccgtaccg aaatcgctgc ggcgaggtgc ctcgccggac
     6841 gacccgcggg ggacccaagc acccgcggcg gatgtgccgc tttggacttc atcgttcctc
     6901 ctcgtgatgg tcatcgttca tacctttttc ctgagtaact ttctgcttac cgccgcgttg
     6961 cccctgtacg ttgccaagtc accacgttgg caagtggggc ttgtcgtcgg ggttccgttc
     7021 atagcatcgc tgattctgcg gccattcacc ggccgcctcg tcgatcgttc cggcaggcgc
     7081 gtctacctga tcacgggtgc agctggctgc gcggcgatga tggcgctgca cgcggtaagc
     7141 gccaatatct ggtttctggc tggggtgcgg ctcttgctgg gagcagcgtc ggcgtttatc
     7201 acgacagcaa tcatggcctc gctcgcggac gtcctccctc ctacccgtcg cggtgctggt
     7261 atggggtggt acggcatctt ttacacgagc accaacctct acggacccgc gcttggtctc
     7321 tggctggcga gcagcttcgg atacgccttc atgtttggct tcgacgcggc gctcaacgtg
     7381 gcggcattgt tggttgccct cgctttgacc gaaacgggcg tacgcaccat aaagggcacg
     7441 ccgcctgcca ggctattcag ccggtcggcc ctgctgccga tgtcgacgtt cctatcgatc
     7501 acgattccag ccggagcagt gaccgctttc cttgcgctcg tggagaaaca acgccatggc
     7561 ggcgacccgg gcctcttttt cctgctgttc ggcgtcactc tgatgtccgg tcgcgtcatc
     7621 ggcggctggg tttcggaccg cttctcgcgg agcgcggcga tcgtgccggg cctggtgcta
     7681 actggcgtgg caatgctggg cctcgccgca gccggagcgc cggtcgcctt ttacctggtg
     7741 gcgctgatgt atggattcgg cttcgcgttg ggtcatacgg gttcgacgat cctcacgatg
     7801 gaccgggcgc ccgaagccga gcgcggtgcg gccatggcga cgctcaccgt tgcgtgggac
     7861 atcgggacgt tggtcggagc cttcatgctt ggctttttgg tcgatgccgc cggctacggc
     7921 gtggtctttt tgcttgtcgg cctaatccca attgcccagg caggtatcta catggtgatc
     7981 gcaaagccgc gccgtagcgt ggtcgcgccc agttaattgc cacttcacgg caggcgcgcg
     8041 gtccacgtcg gtcagcgggc tgattccatg ggttagtagc ggccgatctc gtgcagacgg
     8101 tcatcgtcga tgccgaagtg atgcgcgagc tcgtgtagga cggtgtggcg caccagttcg
     8161 gtcagatgct cttcatcgcg ggcgatgcgt tccagcggct gctggaaaat gaagatgcgg
     8221 tcggggagcg cgaagttata gccgtatccc cgccgcgtga ggtccgtgcc tacgtacagg
     8281 ccgagtagtg tctgtctcgg ctgagtcttc cccgcgtgta gctggtcatc attagcacgg
     8341 gcgcgcacca cgatgtcgat gttttctagc cggccggcaa actccgctgg caagtccttc
     8401 agggcacgac ggacaagcga ctcaaagcga cggcgggaga cggagcggcg ctcgtacatc
     8461 ctggctagct ccgcacgtga cggcgctcga aacatggcag cgcattattc ggcgtgctgc
     8521 tccgagcgga caccttcggg acacgtcgta tggacggtcg cgtcgcagac cgggcaccgc
     8581 cggaacgaaa ggacgacgaa acagcccgat gcaattgagc atgcatgccc gagcggaggg
     8641 tccttgtggc gtagtgcata cggcaggtcg caatggcggc acgtcgtcca gcggatgtac
     8701 tctgagggta gctcaactcg cgaaggcgag gtttggctcg acgtcaaggt cgaagtcctt
     8761 tgcgtctccg gcgctgaagt ggaagtagcc gcaggcgccg accatcgcgg cgttgtcggt
     8821 gcagtacttt ggcggcggaa cgcggacctg caccggcgcc cgggcggtca actctttgcg
     8881 aagagcgcca tttgctgcca ctcctcccgc cacgaggatc tgcttaactc cgaactctcg
     8941 cgccgcgcgg acggtcttgg cggccaacac gtccacgact gattcttgga acgccttggc
     9001 gatatccgcc accggcgggt ggtccggttc gcgcagcatg tggaggacgg cggtcttcag
     9061 accgctgaaa ctgaagttcc atgtccccgg cagccacgcc cgaggaagca cgacgtccgt
     9121 gtggacgatg gtcgcggcgg tccactctat agcgggacca ccgggaaacc ctagctccag
     9181 caggcgggcg actttgtcaa acgcctcacc ggccgcgtcg tcgaccgtgc ggccaagacg
     9241 cttgtactcg ccgtggccac acatgatgat caggtcggtg tggccgcctg aaaccaccag
     9301 acaaagcgca gggaattccg gcggctcggt cggcgtttcg gtgtccaacc agttcgcgta
     9361 gatgtgcgat tcgaggtgat tcaggccgac gagcggcttc cttcgcgcaa atgcaaccgc
     9421 tttggccgcg ttcacgccga cgagcagcga gccggccagt cccgggccgt gggttacggc
     9481 gatgccgtcg atgtcatccc agttgcagcc ggacgagtgc atcgccgtct cgattaccgg
     9541 gatgatcgtt tggagatgtt gtcgggacgc cacttcgggc acgacgccgc cgtaacgccg
     9601 atggacatcg atctgggtgg cgaccatgtt ggacaggatt cgatgaccgt cctcgatgac
     9661 cgccgcggcc gactcatcgc aggatgtctc tacactcagg atacgcatcc aggactactc
     9721 cgggcttagg gggaggcgcg tctaggcatc gtttcggcgt gcgggctggg tttgaccgag
     9781 cagcccctcc tgtgcggcga aggcggctgc ttgctggcgg tttcgcaggt cgagcttttc
     9841 caggatgttg cgcaggtgca ctttcacggt gttgacagcg atgtagaggt gatcggcgat
     9901 ctcctggttg cgccatcccg cggcgacgta ctcgaggatc tcccgctcgc gactggtgag
     9961 ctgatctaat ccaggcccgg cagccctcgg cggcgggctc gccttggcga attcatctag
    10021 cagcctcgtc gcaagtaccg gcgttatcga cgtgccgccg tcagcgagca cttggagcgc
    10081 gtcgtgcagc gccctgagct cggtgtcttt cagcaggtag ccacgcgccc ctgagcggat
    10141 ggccgcgaat aggtcttcgt cctgctcgga cacggtaagc atgcataccg ccgtttcagg
    10201 cgcaagttcc agaatcgtct ttgtagcctc ggtgcccggc atgccgggca tgctcatatc
    10261 catcagcacg acgttaggct tgaacagctt ggccttttcg atcgcctccg ggccgctgcc
    10321 cgcctcggcg acgagctcga cgtcgtcgta ggagttcaac aggctggcaa gcccatcgcg
    10381 gaagaggcgg tggtcgtcca cgaccatgag ccgaacgggt atgcctgtca tgggcatttc
    10441 tccatgcgtg aagcggatat attcgttccc gatcataaca caggcacaca tgacgggcgt
    10501 ctccgtttcc attcgacgta ctggcgagcg atctccgcgc ccagtagcac caggctagcg
    10561 accacatacg cgtaaaacac gaaagcgatc acggctccga gtgaaccata cagcttgtcg
    10621 aagcgaccaa agctgcgcac gtaccagccg aaaccgtagc tggtcgcttg gaagcccgct
    10681 gcggcgacgg cagcaccatg ccagcagtcg gtccagtgaa cgctagccgg tgacgtgacg
    10741 acgtagagct ggctgaacgt tgcgaacgcg attaagaagg gcagaacgag gaagaagacg
    10801 ccgagcccga atgacgttag tcctgcgacg cgggagccaa gcatctcttt cgtcaattcg
    10861 ctcaacgcga caagtccggc aacgccggcc agagcaagcg cgagtaacag gccgaagccg
    10921 attacactga ggaggtccac ggctttggca atgagccagg ggcgcccgtc gctcgtattc
    10981 cagaccacat tgagtccggt gcgaaccgcc gtgaagaccg cgctggctcc ccacaacgcg
    11041 ccgatcaggc cgatcactcc tagctccgcc cggccctttg taaaagactg cacctgttgg
    11101 tagatcttga cggtcgaagc gccgcccagc gcatcggcaa tgttagcaac catgcggtcg
    11161 cggtctgaat tggtgacgaa gtaactgccg aaaccaatga cgaatattga cagcggaagg
    11221 acggagaaca gggtgtagta ggcgatcgcc gccgcgtaac tgcttgcgcg gtcgcccata
    11281 tacgcccgca gcgagcgtcc cactactgaa aacgctgttc gcacagtccc tctgatgatc
    11341 cggttacggc gccgcctgac aaagtcttgt ggctgtcagg ctgctgcttt ctactttacc
    11401 ggtcccggct ttgacgggtt tatatgaggc gcattgttat cacgcttctt gaccggcgtt
    11461 attagccgct cctacactgc gtcagcggac aggcccgggc gccgcccgcg cgccgcccgc
    11521 gcttgcgcct ttcttccagc ttgcaacgag gggaccggat ggcttcgact cgaggtcgga
    11581 ctggcaagcg cggcgccggt cagatgccta cctggcagat tgccacactc aagaatggca
    11641 tgcgcatcgt gaccacgccg attcccacgg cgcaatcggc cagcgtcaac gtatttgtcg
    11701 gcgtcggttc gcgcgccgag gagcgacgcg tcaacggcgt gtcgcacttc atggagcaca
    11761 tgctttttaa gggcacgaac cggcggccga acgcgatcat catcgccagc gaaatcgagg
    11821 gcgccggcgg cgtgctgaac gcctacacga cgaaagaact cacgtgttac tggaaccaag
    11881 ttccctacga catgttgccg ctcgcgatgg acgtcgtagc ggatatggtg cgcaactcac
    11941 tgttggaaga cgttgaggtc gagcgcgagc gaacggtggt acagcaggaa attcgacgcg
    12001 cccatgacca gcctggcgca tgggtcggcg agcttttgtc gggcgccgta tacggcgacc
    12061 agccgatcgg ctggagcatc gcggggacgc tcgatatcgt ggctggcatc tcgcgtgacg
    12121 acatgcgccg gcaccttgat acctggtacg tgccgagcaa cttggtactc agcgtcgccg
    12181 gcaatgtgat ccatcagcag gtagtggagc tatcgcagaa gttgtgggaa gacatgcccg
    12241 gcgacgtcac cccgtctgtg acgccggccg ttccggagat gtcgcctgat cgcattcgta
    12301 tggaccagcg cgacattacg caggcaaaca tggcgatcgg gttgcgctcg ttgcaccgc
//
LOCUS       DATCPE010000056         3506 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U7219
            k141_593127, whole genome shotgun sequence.
ACCESSION   DATCPE010000056 DATCPE010000000
VERSION     DATCPE010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955033
            Sequence Read Archive: SRR1586279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 16:49:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 640
            CDSs (total)                      :: 631
            Genes (coding)                    :: 627
            CDSs (with protein)               :: 627
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3506
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_593127"
                     /isolate="SMAG_U7219"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            17..1453
                     /gene="lpdA"
                     /locus_tag="VIM45_01770"
     CDS             17..1453
                     /gene="lpdA"
                     /locus_tag="VIM45_01770"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEY8820047.1"
                     /translation="MTIINIEDVYTSRGSSMDEFDVVVVGGGPGGYVAAIRAAQLKLR
                     TALVERDQVGGICLNWGCIPSKALLRNAEILHLFHRAAEFGIAAEGVRGDFGHAIDRS
                     RGVVDRMVKGVQFLLRKHGVEVFAGSAALCSANEIEVSDGRQIRSKNIIVATGARSRN
                     LPGLEIDRHRMITSREALERRDRPGSIVIVGAGPVGMEFAYVYRNYGSEVTMVEALSH
                     LLPQEDEAISMVIEKEFDKLGINVRLNTTVLGARPGANAVEVDVEGRDGRATVTADVV
                     LVGIGVRGNSDGIGLEQLGVVTEQSFIKIDEHMATNVPGVFAVGDVTGPPMLAHVASA
                     QGVNAVEGIAGLHPPALDYEQMPRATYCQPEVASIGLTEAQAKDRGLAYKVGTFPFRA
                     NGRALAMAEPEGLAKVIADEQSGDLLGFHLVGPGVTELLGELSLARSLEATPEAIGYT
                     VHAHPTLSEAVKEAALSARGEVIHVWQG"
     gene            1489..2601
                     /gene="pdhA"
                     /locus_tag="VIM45_01775"
     CDS             1489..2601
                     /gene="pdhA"
                     /locus_tag="VIM45_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008475609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HEY8820048.1"
                     /translation="MVVTVQATTSQNGVAPLDQTHGDGRDVTELNDDQLVTWLRQMQL
                     IRRFEERAAEMYTRRKIGGFLHLYVGEEAVAVGTIARLRPQDYIVSHYRDHGHAIARG
                     LPPGAIMAELFGRSTGVSHGKGGSMHLFDRARHFLGGYAIVAGHLPIACGLGLSAQTQ
                     GEDRVVLCIFGDGAVNEGEFHEALNLASLWKLPVIFLCENNLYGMGTALAAASATSEL
                     YKFAEAYNMPGARCNGMDVRDCYRSMGDALAHCTSGKGPYFLEAMTYRFRGHSMADPE
                     LYRTKDEVAEWRRKDPILMLRQDLVSREVLTEDDVTRMFQDVESEVDEATMFADESPK
                     PDPSQLYTDVYAESVRSHVALAHRSKVGETTHGDSE"
     gene            2605..>3506
                     /locus_tag="VIM45_01780"
     CDS             2605..>3506
                     /locus_tag="VIM45_01780"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEY8820049.1"
                     /translation="MTTMTPVREEQQPAGNGSADAGETVEMTIRDAIALALREELIAD
                     EHVFLMGEDIGAYGGSYVVTKGFFEEFGDKRVRDTPIAESVIVGTAIGAAMGGLKPIV
                     ELMTINFSLLAFDQIVNHAAKILYMSGGQVNVPMVLRMVTGGGSQLGAQHSQNLEAIF
                     ARVPGLTVVCPSTPYDALGMMRQAVQEANPYIFVEHSLLYRTKGLVPKAYYTVPSGKS
                     AVRRRGKNVTLVGYLRSVVLNLEAAEVLAQEGIEAEVIDLRSLRPLDLEPVIASVKET
                     NRVVIAEDAWMTGGISSEIACQIQ"
BASE COUNT          698 a         1034 c         1145 g          629 t
ORIGIN      
        1 gcccgcttta taaccattga ctataataaa catcgaagat gtctatacga gtcgcgggag
       61 ttcgatggac gagttcgacg ttgtggtggt gggtggcggt ccaggcggct acgtcgcggc
      121 catacgggcg gcccagttga agcttcgcac tgccctcgtc gagcgggacc aggtgggcgg
      181 catctgcctc aactgggggt gcatcccttc caaagcgttg ctccgcaacg ccgagatcct
      241 tcacttgttt catcgggcag cggaatttgg catcgccgcc gagggcgttc ggggcgactt
      301 cggccacgcg atcgaccgca gccgcggcgt tgtggaccgt atggtcaaag gcgttcagtt
      361 cctgcttcgg aaacacggcg tcgaggtatt cgccggcagc gcggccctct gttctgcaaa
      421 cgaaatcgaa gtcagcgacg gccgccagat ccggtcgaag aacataattg tcgcaaccgg
      481 agcccgcagc cgtaatcttc cggggctgga gatcgaccgg catcggatga tcaccagccg
      541 cgaggcgctc gaacgccgcg accgtcccgg ctcgatcgtc atcgtcggcg ccggacccgt
      601 aggcatggaa ttcgcttacg tctaccgcaa ctacgggagc gaagttacga tggtcgaagc
      661 gctgtcccac cttctgcccc aggaagacga ggcaatctca atggtcatcg aaaaggagtt
      721 cgacaaactc ggcatcaacg ttcgcctgaa cacgacggtg ctaggcgcac ggcccggtgc
      781 gaacgccgtt gaagtggatg tcgaggggcg agacggccgc gcgaccgtga ccgccgacgt
      841 cgtactcgtt ggcattggcg tacggggaaa cagcgatggc attgggctgg aacagttagg
      901 cgtggtgacc gagcaatcct ttatcaagat cgacgagcac atggcgacca acgtgccggg
      961 tgtgtttgcc gtcggtgacg tgaccggtcc gccgatgctt gctcacgtgg cctccgcaca
     1021 gggcgtcaac gcggttgaag gaattgcggg actgcatccc ccggcgctcg attacgagca
     1081 gatgccgcgt gccacgtact gccagcccga ggtcgcgagc atcggtttga ccgaagcgca
     1141 ggcgaaagat cgaggtctcg cgtataaagt cggaaccttt cccttccgcg ccaacggccg
     1201 cgccctggca atggccgagc cggaggggct ggccaaagtc attgctgatg aacagtcggg
     1261 cgatctgttg ggatttcatc ttgttggtcc cggggtcacc gagcttctgg gggagctttc
     1321 gctcgctcgc tcgctcgaag cgacgcccga ggcgatcggc tacacggttc acgctcatcc
     1381 aacgctgtcc gaggcagtga aagaagcagc gctttccgcg cgtggtgaag taatccacgt
     1441 ctggcagggt tgaagtcgaa cgggccgctg gtactacggg aggcagggat ggtggtcacg
     1501 gtacaagcaa ccacttcaca aaatggcgtc gcgccgctgg atcagaccca cggcgatggc
     1561 agagacgtta cggagctaaa cgacgaccag ctcgtcacat ggctaaggca gatgcagctc
     1621 atccgccgct tcgaggagcg cgcggcggag atgtacaccc gtcggaagat cggcggcttt
     1681 ttgcacctct acgtcggcga ggaggcggtc gcggtcggca cgatcgcgcg tctgcggccg
     1741 caggactata tcgtcagtca ctaccgcgac cacggtcacg cgatcgcccg cggcctgccg
     1801 ccgggcgcaa tcatggccga gctttttggc agatcgacgg gcgtcagcca tggcaaaggt
     1861 gggtcgatgc acttgttcga ccgcgcccgg cacttcctcg gtggctatgc gatcgtggcg
     1921 ggccacctgc cgatcgcgtg cgggctcggc ctttcggcgc agacgcaggg cgaggaccga
     1981 gtggtgctct gcattttcgg cgacggagcc gtcaacgaag gggagtttca cgaagcactc
     2041 aacctggcat cgctgtggaa gctgccggtg atcttcctgt gtgaaaacaa cctctatggc
     2101 atgggcaccg cgctggcggc agcctcggcg acgagcgagc tgtataagtt cgcggaagcg
     2161 tacaacatgc ccggcgcccg ctgcaacggc atggatgtcc gcgattgcta tcgatcaatg
     2221 ggcgatgcgc ttgcgcactg caccagcggc aaagggccat acttcctcga ggcaatgacc
     2281 tatcgtttcc gcggccattc gatggccgac ccggagcttt atcgcaccaa ggacgaagtc
     2341 gccgagtggc gccggaagga tccgattctg atgcttcggc aggatctcgt ttcccgggag
     2401 gtccttaccg aagacgacgt gacgcggatg tttcaggacg tcgagagcga ggtcgacgag
     2461 gcgacgatgt tcgccgacga gagccccaag ccggacccat cgcagcttta taccgacgtc
     2521 tacgccgaga gtgttcgcag ccacgtcgcg ctggcacacc gcagcaaggt aggcgaaacc
     2581 acgcacggcg attcggagta aacgatgacc acgatgacac ctgtccgcga ggagcaacag
     2641 ccggccggta acgggtccgc ggatgccggc gaaacagtag aaatgaccat tcgcgacgcg
     2701 atcgccctgg cgctgcgcga agagttgatc gccgatgaac acgtattcct gatgggagag
     2761 gacattggcg cctacggggg ttcctacgtc gtcaccaaag ggttcttcga ggagttcggc
     2821 gacaaacggg tccgcgatac gccgatcgcc gagtctgtga tcgtcggcac ggcgataggc
     2881 gccgcaatgg gtgggctgaa gccgatcgtc gagctgatga cgattaactt cagcctgctg
     2941 gccttcgacc agatcgtcaa ccatgcggcc aagatcctgt acatgtccgg cggccaggtc
     3001 aacgtgccga tggtcctgcg catggtgacc ggcggcggca gccagcttgg cgctcagcat
     3061 tcacagaacc tggaggccat ttttgcacga gtgccggggc tgacggtggt ctgtccttcc
     3121 acgccgtatg acgcactcgg gatgatgcgc caggccgtgc aggaggcgaa cccgtatatc
     3181 ttcgtcgaac atagcctcct gtacaggacg aaaggcctgg taccgaaggc ctattacacg
     3241 gtgccaagcg gcaaatccgc cgttcggcgc aggggcaaga acgtgacgct ggtcggctac
     3301 ctgcgctccg tagtgctcaa cctcgaggca gccgaagtat tggcgcagga agggattgag
     3361 gccgaagtca tcgacctgcg atcactacgc ccgctggacc tggagccggt catcgcatcg
     3421 gtgaaggaga caaaccgcgt ggtaatcgcc gaggacgcgt ggatgaccgg cggaatttcg
     3481 agcgaaatcg cctgtcagat acagga
//
LOCUS       DATCPE010000057         5330 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U7219
            k141_560784, whole genome shotgun sequence.
ACCESSION   DATCPE010000057 DATCPE010000000
VERSION     DATCPE010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955033
            Sequence Read Archive: SRR1586279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 5330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 16:49:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 640
            CDSs (total)                      :: 631
            Genes (coding)                    :: 627
            CDSs (with protein)               :: 627
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5330
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_560784"
                     /isolate="SMAG_U7219"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2449
                     /locus_tag="VIM45_01785"
     CDS             <1..2449
                     /locus_tag="VIM45_01785"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY8820050.1"
                     /translation="AVEDSVTRVQRSLRQFDREREDNAGRRNQMLARLRKHGGDALRL
                     ITDQRRFAAELAARQVEQGTTRRQAQLAREATERADADLAGLAHQLDAARSRLELLAR
                     LRDEHRNSDGALRALVHAAQRVGPETPPRLVAVLSQVIRVQKGLETAIEAALGEQLSA
                     IVVPETLDAQAALAVLEESRAGRATFVPMSELKASHALNLTSERGVLGVASRFIKCDA
                     RYRELIDTLLGRVIVVDDIRAGQAMLRRGLGTAVTLDGTVLRPTGIITGGPAGSAGLV
                     LEFERDLEELPGQVEELREAHAARKQDAERMREAMRIAEARLRDGESRIETLQSALRR
                     LEGEAGMVTGAIAGLQGEAAILRGNGAAFADRTAELNRELAEAEEERRRLSLGVRSAQ
                     LGVEAARREVEGLAVPVEELRNSLAVVTGARQALEQQRTALARMIEGRRTTLERTERT
                     MATREAELANRRIELEQIVQQQGQARDQLASWTDTLSEQRAILQPIRERVTVLSRRER
                     ELFERFTHGRTELLAFERASMEAEAAVNRRAQAIDRLREEMEADEIESPDELSSAEAG
                     LEMVAARAFGGDPTRVDQATTGVGEDRIDPSSLQSRVRSLRGSIRGLGPINAQAEADY
                     DEARERHDFLTSQVQDLEQAEASLQEALEELRRVVRDRFRETFHAVNADFARYFKTFF
                     GGGTARLALTEPEDYGESGVDILAQPPGKRQQHLQLLSGGERSMTAVALLFALLETNP
                     APFCVLDEVDAALDEANVSRFGDALGELARRTQFILITHNRSTIQAAQSIYGISMGSD
                     GVSTVLSLRLEDLPVGA"
     gene            2729..3730
                     /gene="ftsY"
                     /locus_tag="VIM45_01790"
     CDS             2729..3730
                     /gene="ftsY"
                     /locus_tag="VIM45_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008517022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEY8820051.1"
                     /translation="MIDGLDGVEPEPVEPEEGPAFDEAVDERTEQALGRTKRGFFQRM
                     VGVFERSQIDDELWDELEEILIGADTGVETTEMILDEVRSRVRKEGLKQPEEVREALR
                     DQLIALLESVDGKGQLWGDQSDDDRSFSPAVILVVGVNGAGKTTSIAKLARAFRRDGE
                     RVVLAAADTFRAAAIEQLKLWGDRVGATVIAHQQGQDASAVVFDAMAAAKKRAADVVL
                     IDTAGRLHTKSNLMEELKKINRIVQRSDDTAPHEVLLVIDAVTGQNGLLQAKSFAEAV
                     GVTSIFLTKLDGTAKGGIVFSIADQLRIPVRFIGTGERADDMAPFDAESFVDALFSE"
     gene            3740..>5330
                     /locus_tag="VIM45_01795"
     CDS             3740..>5330
                     /locus_tag="VIM45_01795"
                     /inference="COORDINATES: protein motif:HMM:NF021570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2298 domain-containing protein"
                     /protein_id="HEY8820052.1"
                     /translation="MAYLVRWWLASEIIGLVTLPLTFRIFRRLPDRGYTFSRIIGLLL
                     AGYVLWIGGTAGVLPFSAGSVVAVVLVFGIVGAWLFARDRGEIADWLRASKRYVVVAE
                     AVFLGAMLAIAFLRSYQPDIANTEKPFEYANYQAVNRSVYFAPSDPWFAGKPMPYYYL
                     GYEIGDSVAKVTGTPSNYAFNLALVLVGGMAALAAFGVAATAAVLLRTSGTARDTWPI
                     VAGLFAVLLLMVIGNLEGWFELAAVHGWNPQWVYQHLDVSTYGGLLPRHSSHWWPDES
                     FYAAGWRATRLGSTWNFLEFPFFSFLLGDVHPHVLALPFKLLCAGLALAVLTATHLPH
                     PGVWRDGLSVREQLLDRRLRLLDLRRWQWSGWLVWAAAALAAGSLIAIHTWDYPPFLL
                     LIGAMVVARTAISKERDWIGAGACAAGLAILSLLFMLPFFASTGHGGIQGISATEVAF
                     RSPAVEPEGAYLPFQHLLIFWLPLMFPTAIFVAWCLAQRGWRPVREYGATMITLVSVL
                     ALAWGVTILGSHGGSGLRQELQ"
BASE COUNT          956 a         1636 c         1789 g          949 t
ORIGIN      
        1 cgccgttgaa gacagcgtca cgagggttca gcggtcgctg cgccagttcg atcgggaacg
       61 cgaggataac gccggccgcc gcaaccagat gctggctcgg ctgcgcaagc acggcggcga
      121 cgcgctccgg ttgatcacag accagcggcg gtttgcagcc gaactggcag cgcggcaggt
      181 tgaacaggga acgacccgac gccaggcgca actcgcgcgc gaggcgaccg agcgtgccga
      241 cgcggacctt gccggactag cacaccagct tgatgcggcg cggtcgcggc tggagcttct
      301 ggcacgcttg cgcgacgagc accgcaacag cgatggcgcc ttgcgcgctt tggttcatgc
      361 agcccaacgc gtcggaccgg agacgccccc acgtctggtt gccgtgctct cgcaggtcat
      421 ccgcgttcaa aagggtctcg aaacggcgat cgaagcggca ctcggcgaac agctgtcggc
      481 gatcgtcgtg cctgagacgt tggacgccca ggccgcgctt gccgtgctcg aagagagccg
      541 cgccgggagg gccacgtttg tacccatgtc cgaactgaag gcgtcgcacg ccttgaacct
      601 gaccagcgag cgtggagtgc tcggcgtcgc gtcgcggttc atcaagtgcg acgcgcgcta
      661 ccgggagctc atcgacacgc tgctcggtcg tgtgatcgtc gtcgacgaca tccgcgccgg
      721 acaggcgatg ctgcggcgcg gcctcggcac cgcggttacg ctggacggaa ccgtgctgcg
      781 accaacgggg atcatcaccg gggggccagc ggggtcagcc ggtctcgtgc tcgaattcga
      841 gcgtgacttg gaggaattgc ccgggcaggt cgaggaatta cgggaagcgc acgcagcccg
      901 caagcaagat gccgaacgca tgcgcgaagc gatgcgtatc gccgaggcgc gactgcgaga
      961 cggcgagagc cggatcgaaa ccctgcaatc tgcccttcga cggctcgagg gcgaggcagg
     1021 aatggtcacg ggggcgatcg cgggattgca gggtgaggcg gcgatcttgc gcggcaacgg
     1081 cgcggccttt gctgaccgga cggcggaact gaatcgcgag cttgcagaag cagaagagga
     1141 gcgacgcaga ctgtcgctgg gggtgcgatc ggcgcagttg ggcgtggaag ccgcacgacg
     1201 cgaagtcgaa ggcctcgcag tgcccgtgga agagctgcgc aacagtttgg ccgtggtaac
     1261 cggggctcgc caggcattgg agcagcagcg gacggcgctg gcgcggatga tcgaagggcg
     1321 ccggacaacg ctggaacgaa ctgagcgcac gatggccacg cgcgaagcgg agctcgctaa
     1381 tcgcaggatt gagctcgaac agatcgtcca gcagcaaggt caggcccgcg accaactagc
     1441 gtcgtggact gacaccctgt cggagcaacg agccattctc cagccaatcc gcgaacgcgt
     1501 aactgtacta tctcgccgcg aacgcgaact attcgagcgg ttcacccacg gccgcacgga
     1561 gttgttggct tttgaacggg cgtcgatgga ggccgaagcc gcggtgaatc ggcgggcgca
     1621 ggcgatcgat cgcctgcgcg aggagatgga ggcggacgaa atagagagcc ccgacgagct
     1681 ttcctccgcc gaagcagggc tcgaaatggt cgccgcgagg gccttcggcg gggatcccac
     1741 ccgcgtcgat caggcaacga ccggggtcgg cgaggaccgg atcgatccgt cctcattgca
     1801 gtcgcgagtg cgctcgctgc gggggagcat ccgcggtctc ggcccgatca acgcccaggc
     1861 ggaggccgac tacgacgagg cccgcgagcg tcacgatttt ctcacgtcgc aggtgcagga
     1921 cctggagcag gccgaagcct cgttgcaaga ggcgctcgaa gagttgcgcc gtgtcgtgag
     1981 ggatcgcttt cgggagacct tccacgcggt gaacgcggac tttgccaggt acttcaagac
     2041 gttctttggc ggcggcaccg cccggcttgc gctgactgag ccggaggact acggagaaag
     2101 cggcgtggac atcctggcgc agcctccggg caaacgccag caacacctgc aactgctcag
     2161 cggcggcgaa cgctcgatga ccgcggtcgc cctgctgttc gcgctcctgg aaaccaaccc
     2221 ggcgccgttc tgtgtgctcg atgaggtcga tgccgcgctc gacgaggcaa atgtcagccg
     2281 cttcggcgac gcccttggtg aactggcaag gagaacacag ttcatcttga ttacgcacaa
     2341 tcgcagcacg atccaggccg cccaatctat ctatggcatt tcaatgggca gcgacggggt
     2401 ttccacggta ttgtcgcttc gcctggagga tcttccggta ggcgcctaac ccgatatcgc
     2461 gggcacacct cccggccttg ttgcaagtcg gaagccgttg atatgatccc cgaaacggtc
     2521 cgtacggccc tgcgcagctc aggcaaatat tggcgaccga ggcactcccc tgttcaagct
     2581 ttttcgccgc aagcagaacg aaaatgaaga tggcacgggc aacgacactg ttgtcggcga
     2641 aggcgcagac gtcactgaat gcagccgaac cggcatcgcg gtcgccgagc ttgcggacgc
     2701 tgacgccgag ccggagacgg ccggcctggt gatcgatggt ctcgacggcg tcgagcccga
     2761 gccggtagaa cccgaagaag gtcccgcgtt cgacgaagcg gtcgacgagc gaaccgagca
     2821 ggcgctcgga cgcaccaagc gcggattctt ccagcggatg gtcggcgtat tcgaacgctc
     2881 ccagatcgac gacgaactat gggacgagct cgaggagatt ctgatcggcg ccgacacggg
     2941 ggtcgaaacg acggagatga tactcgacga ggtgcgaagc cgggtccgca aagagggctt
     3001 gaagcagccc gaagaggtgc gagaagcgct ccgcgatcag ctcatcgcgc tactggagtc
     3061 ggttgacggc aagggacagc tctggggtga ccagagcgat gatgatcggt cgttctcgcc
     3121 cgcggttatc ctcgtggtcg gcgtcaacgg tgcaggaaag acgaccagca ttgcgaagtt
     3181 ggctcgcgcc ttccgcaggg atggcgagcg cgtcgttctt gccgccgcag acacgttccg
     3241 cgccgccgca atcgagcagc tcaaactctg gggagaccgg gtaggcgcaa cggttatcgc
     3301 acaccagcag gggcaggacg cctctgccgt cgttttcgat gcaatggcag cggcgaagaa
     3361 gcgggccgcc gacgtcgtcc tgatcgatac cgcgggccgc ctgcatacca agtcaaacct
     3421 tatggaagag ctgaaaaaga tcaaccgcat tgtccagcga agcgacgata ctgcgcccca
     3481 cgaggtctta ctcgtgatcg atgcggttac cggccagaat ggactgctgc aggcaaagtc
     3541 gttcgccgaa gccgtcggcg tcacgtcgat cttcctgacc aagctggacg gaacggcgaa
     3601 aggcggcatc gtcttctcaa tcgccgacca gttgcggatc cccgtgcgct tcattggcac
     3661 cggtgaaagg gccgacgata tggcgccgtt cgacgccgaa agcttcgtcg acgcgctctt
     3721 ctccgaatag gtgctgtaga tggcgtacct ggttcgctgg tggctggcct cggaaatcat
     3781 cggcttggtc acgttgccgc tcacatttcg tatctttcgt cgtctgccag accgcgggta
     3841 tacgttctcg cgcattatcg gcctgctgct ggccggctat gtgctgtgga tcggcggaac
     3901 ggcgggcgtc ttgccctttt cggcgggttc agtcgtcgcc gtcgttttgg tattcgggat
     3961 cgtcggcgcc tggctgttcg cgcgcgaccg cggtgagata gccgactggt tgcgagcttc
     4021 gaaacgctac gtggtcgtag ccgaggctgt tttcctcggc gccatgcttg cgatcgcatt
     4081 ccttcggtcc tatcagccgg atatcgccaa cactgagaag ccgtttgagt acgccaatta
     4141 ccaggcggtc aaccgtagcg tgtatttcgc accgtccgac ccatggtttg ccggcaagcc
     4201 gatgccttac tactacctcg gttacgagat cggcgatagc gtggcaaagg tcacgggtac
     4261 gccatctaac tacgctttca acctggcgct ggttctggtc ggcggcatgg cggcgctggc
     4321 ggcgtttggg gtggcagcta cggccgcggt cctgcttcgc acgtccggaa ccgcccgtga
     4381 cacatggccg atcgtcgccg ggctgttcgc cgtacttctg ctcatggtga tcggcaatct
     4441 ggaaggttgg tttgagcttg ccgccgtgca tggttggaac ccgcaatggg tctaccagca
     4501 tctcgacgtc tcgacctacg gcggactgct gcctcggcac agctcccact ggtggccgga
     4561 cgagtcgttc tatgcagccg gctggcgggc gacccgcctg ggctcgacgt ggaacttcct
     4621 tgagttccca tttttcagct ttctgctcgg cgatgttcac ccacacgtgc tcgccctgcc
     4681 gttcaaactg ctctgtgccg ggctggcgct cgccgtgttg accgccaccc acctgccaca
     4741 ccccggcgtt tggcgcgacg ggttatcagt gcgagagcag ctactggatc gccggctccg
     4801 gcttctcgat ctccggcgct ggcagtggag cggctggctg gtatgggcag ccgcagcgct
     4861 tgcggccgga tcgttgatcg cgatccatac ctgggattac ccacccttcc tgctgctcat
     4921 cggcgcgatg gtcgtcgcgc ggacggcgat atcgaaagag cgcgactgga tcggcgccgg
     4981 tgcgtgcgct gccgggctgg ccattctgtc gctgttgttc atgctgccgt tcttcgcgag
     5041 cactggccat ggcgggatcc agggcatttc ggcaaccgaa gtggcgtttc gaagccccgc
     5101 cgtcgagccg gaaggcgcct acctgccttt ccaacacctt ctgatattct ggctgccgct
     5161 aatgttccca accgccattt tcgtcgcctg gtgcctcgcg cagcgcggct ggcgaccggt
     5221 gcgcgagtac ggggccacga tgatcaccct cgtgtcggtg ctggcgctgg cctggggagt
     5281 gacgatcctg ggctcacacg gcggatctgg gctgaggcaa gagctgcaga
//
LOCUS       DATCPE010000058         3183 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U7219
            k141_269174, whole genome shotgun sequence.
ACCESSION   DATCPE010000058 DATCPE010000000
VERSION     DATCPE010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955033
            Sequence Read Archive: SRR1586279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 16:49:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 640
            CDSs (total)                      :: 631
            Genes (coding)                    :: 627
            CDSs (with protein)               :: 627
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3183
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269174"
                     /isolate="SMAG_U7219"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1039
                     /gene="purM"
                     /locus_tag="VIM45_01800"
     CDS             <1..1039
                     /gene="purM"
                     /locus_tag="VIM45_01800"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEY8820053.1"
                     /translation="SYSAAGVDIDATNRAVERMKALAAGASRPEVLAGIGPFAAMFHM
                     GTYRDAVLVSSTDGVGTKVKLARALGRYDTVGRDLVNHCVNDILTAGAEPLFFLDYLA
                     NSGLTEDQKVALVGGMAGACRDVGCALVGGETADMPDVYEPGDFDIAGFIVGVAERDA
                     LMDGSRIVPGDVLLALPSNGLHTNGYSLVRTVFDVARGGAEREAADREKLARLVPGTS
                     TSLGEALMVPHHCYLHELRPVLRHIKAMAHITGGGLFDNVPRILPDGVAAEFDRTSWK
                     VPPLFGYIQREGNVLDAEMFRTFNMGVGMVLAVDPASRDVILSHDRLPDAWEVGCVIA
                     NNADGPRVLLR"
     gene            1141..2667
                     /gene="purH"
                     /locus_tag="VIM45_01805"
     CDS             1141..2667
                     /gene="purH"
                     /locus_tag="VIM45_01805"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012882455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEY8820054.1"
                     /translation="MRAILSVYDKTGIAEFAAGLNQLGAEIFSTGNTKTAIAAAGVPV
                     HGISDLTGFPEILDGRVKTLHPAVHGGLLARRDLPEHMRELEANGLQTVDLVAGNLYP
                     FVQTVAGGADLTAALDNIDIGGPTLLRSAAKNFPSVVVIVDPDDYGPVLEALRSGGPP
                     LEERQRLAQKAFQHVASYDTAIAEYLRGSVKLFPQYLTKAYQKRFNLRYGENPHQQAA
                     FYVEQSVFSTRPAGIAGARQLHGKELSFNNILDADAAWNTVREFPGQAVAVVKHTNPC
                     GLAEHENQAEAYRRAFAGDEVSAFGGIVAVNRPLDREMAEAMRPVFYEIVIAPSYTDE
                     ALATLRKKRDLRILEVDAEVDGAALDYRRVRGGMLLQTPDEFPEEQIEFTTQSERRPT
                     PAELDDLRFAWGTVKHVKSNAIVLVKDRAIVGIGAGQPNRVVSVHLAVRSAGDRAKGS
                     VLASDAFFPFPDGPQLAADAGVTAIVQPGGSIRDTETIAVCNAADIALVHTGVRHFRH
                     "
     gene            2762..>3183
                     /locus_tag="VIM45_01810"
     CDS             2762..>3183
                     /locus_tag="VIM45_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8820055.1"
                     /translation="MAITREQTIAELDEAVQHMSHGPRSLFERIFSVQSSHGRLIPPA
                     EMEPWILKQFGSLEATVDQKIIRITNNITLEGVLFNWLRSSRPMWNAPALDLDGELDK
                     INANDPLGDPYHGTPEDVFGRIEGRYCVTASNIAKFDG"
BASE COUNT          601 a         1017 c         1037 g          528 t
ORIGIN      
        1 ctcgtacagt gccgccggag tcgatatcga cgcgacgaac cgcgcggtcg agcgcatgaa
       61 ggcgctcgcg gccggcgcgt cgcggccgga ggtgctggcg gggatcggcc ccttcgccgc
      121 catgttccat atgggcactt atcgcgacgc cgtgctcgtc tccagcacgg acggcgtcgg
      181 cacgaaggtg aagctcgcgc gcgccctcgg ccgttacgac accgtcggcc gcgacctcgt
      241 gaaccattgc gtcaacgaca tcctgaccgc cggcgccgag ccgctcttct tcctcgacta
      301 cctggccaat agcggtctca ctgaagatca gaaggtagcg ctggtcggag ggatggccgg
      361 cgcatgccgg gacgtcggtt gtgcgctggt cggcggagaa accgccgaca tgccggacgt
      421 ctacgagccg ggcgacttcg acatcgccgg cttcattgtc ggcgtggccg agcgagacgc
      481 gctcatggat ggcagccgca ttgtgcccgg cgacgtgttg ctggcgctcc catcaaacgg
      541 cctgcacacg aacggttatt cgctggtcag aaccgtgttc gacgtcgccc gtggcggcgc
      601 cgagcgtgag gcggccgaca gagaaaaact ggcgcggctc gtccccggca cgtcgacgag
      661 cctgggcgag gcgctcatgg tgccgcatca ctgctacctg cacgagctga ggccggtgct
      721 gcgccacatc aaggcgatgg cgcacatcac cggcgggggg ctgttcgaca acgtgccacg
      781 tattctgcca gacggtgtcg cggccgagtt cgaccgcaca agctggaagg tgccgccgct
      841 cttcgggtac attcagcgcg agggcaacgt actcgacgcg gagatgttcc gcacattcaa
      901 tatgggggtg ggaatggtgc tcgccgtcga ccccgcgtcc cgcgatgtga tcctcagtca
      961 tgaccgcctc cccgacgcct gggaagtggg atgcgtcatc gccaacaatg ccgacggccc
     1021 gcgggttctg ctgcgctaac agctcgaacg gggttgccga tgacggacgg cggcaccctg
     1081 tggggcgccg tcttttaatg tccgccgctc ggacggcatg gtagagagga cgaagcgatc
     1141 atgcgagcca tcctcagcgt gtacgacaag acgggaatcg cggagtttgc cgcgggcctg
     1201 aaccaactag gcgccgagat cttcagcacc ggtaacacca agacggcgat cgccgcggcc
     1261 ggagtgcccg tacacggcat ctccgacctg accggctttc cagagatcct cgacggacgc
     1321 gtgaagacgc tccatccggc cgtccacggc ggcctgctgg cgcgtcgcga cctgcctgag
     1381 cacatgcgag aactggaggc gaacggccta caaacagtcg acctcgtcgc gggaaacctc
     1441 tatccattcg tgcagacggt tgcgggcggc gccgacctga cggccgctct cgacaacatc
     1501 gatatcggcg gtccgacctt gctgcgctcc gccgccaaga actttccgag cgtcgtggtg
     1561 atcgtcgacc ccgacgacta cggcccggtt ctggaggctc tgcgatcagg gggaccgccg
     1621 ctcgaagagc ggcaacggct ggctcagaaa gcgttccaac acgtcgcaag ctacgacacg
     1681 gcgatcgccg aatacctgcg cggatctgta aagcttttcc cgcagtactt gacaaaggca
     1741 taccaaaaac gtttcaatct gcggtatggg gagaatcccc atcagcaggc ggccttttat
     1801 gtcgaacagt cggtcttctc cacgcggccg gccggaatcg cgggcgcacg ccagctacac
     1861 gggaaggaat tgtcgttcaa caacatcctg gatgcggacg ccgcctggaa caccgtacgt
     1921 gagttccccg gacaggccgt cgcggttgtg aagcatacga atccatgcgg gctggccgag
     1981 catgagaacc aggcggaagc ctaccgtcgc gcctttgcgg gggatgaggt ctcggcattc
     2041 ggcggcattg tggcggtaaa tcgtcccctc gaccgggaga tggctgaggc gatgcgaccg
     2101 gtgttctacg agatcgtcat cgcgccgtcg tacaccgacg aggcgctggc aaccttgcga
     2161 aagaagcgcg acctccgcat cctcgaagtg gacgccgagg tagacggcgc cgcgctcgat
     2221 taccgtcgcg tgcgtggcgg catgctgctg cagacgcccg acgaattccc tgaggagcaa
     2281 atcgagttca cgacgcagag cgagcgcagg ccgaccccgg ccgaattgga cgatctgcgc
     2341 ttcgcgtggg gcaccgtcaa gcacgtcaag tccaacgcga tcgtcctggt caaggaccgg
     2401 gcgatcgtgg gcatcggcgc cggccagcca aaccgcgtgg tgagcgtgca cctcgccgtg
     2461 cggtccgctg gcgaccgggc gaagggctcg gtgctcgcgt cggacgcgtt cttcccattc
     2521 ccggacggcc cgcagctcgc ggcggatgca ggcgtaacgg ccatcgtcca gccgggcggg
     2581 tcgattcgcg acactgaaac gatcgctgtc tgtaacgccg cggacatcgc cctggttcac
     2641 accggggtca ggcacttccg gcattagcac gcgttgcaag ccgcaaaggt gaaaaggacg
     2701 ccttgtatca ggacaataat tggcaggact ccccgcagca agggagggat ggagtgaaac
     2761 gttggccatt acccgtgagc agacaatcgc ggagttggac gaggccgtgc aacacatgtc
     2821 gcatggtcca cgcagcctct tcgagcgcat tttcagcgtg caatccagtc atgggcggct
     2881 gattccgcct gccgagatgg aaccgtggat cctcaagcag ttcggcagtc tcgaggcgac
     2941 cgtcgatcag aagatcatcc ggatcaccaa caacatcacg ctggaaggcg tgctgttcaa
     3001 ctggctccgc tccagccggc cgatgtggaa tgcgcccgcg ctcgacctcg atggagagct
     3061 ggacaagatc aacgccaatg atccgctggg agacccctat cacggcaccc cggaggatgt
     3121 cttcggccgc atcgagggac gctactgcgt cacggcgagc aacatcgcca agttcgacgg
     3181 ctt
//
LOCUS       DATCPE010000059         7349 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U7219
            k141_496545, whole genome shotgun sequence.
ACCESSION   DATCPE010000059 DATCPE010000000
VERSION     DATCPE010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955033
            Sequence Read Archive: SRR1586279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 7349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 16:49:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 640
            CDSs (total)                      :: 631
            Genes (coding)                    :: 627
            CDSs (with protein)               :: 627
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7349
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_496545"
                     /isolate="SMAG_U7219"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(227..529)
                     /gene="yajC"
                     /locus_tag="VIM45_01815"
     CDS             complement(227..529)
                     /gene="yajC"
                     /locus_tag="VIM45_01815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEY8820056.1"
                     /translation="MAIMLQLMVTSAILLGGFYYILLRPVLQQQRKTKRDLLELQTGD
                     EVVTTGGLIATVIDIRQPAVGPIELVLELAPGMEVRALTTAILERRTQPEPVPAAP"
     gene            complement(534..2042)
                     /locus_tag="VIM45_01820"
     CDS             complement(534..2042)
                     /locus_tag="VIM45_01820"
                     /inference="COORDINATES: protein motif:HMM:NF013871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEY8820057.1"
                     /translation="MVSAAECIQLIPPELLSLLAASEDTRAYLVGGFVRDALLGRQNH
                     DLDLAVHGDPLSMARRIGARANGAVVALDPDRGTYRVVLREPMEQMRTVDLTRLRAPT
                     LEGDLAARDFTINAIAVPVDGEGPSFIDPTGGLGDLRRKRVKVASPESFIDDPVRLLR
                     VVRFGLELGFTLDQQTQELARAAASLLPRAAAERRRDEFMRLFECDNAASGIRTLDSL
                     RLLTTMLPDLEATRSVSQPKEHYYDVFEHSLHTLAALDLMLRRAPPTHKVDATLWRAL
                     WDGFDWVPGLPQRLEAQLGEGRTRRSMLKVAGLLHDISKPETKTVDSQSGNMRFYGHP
                     ELGARRTMDTLGALRLSAHEIQYAGLLIAQHLRPGMLAGPGEAPTRRALFRFFRDLGD
                     ASPDLLLLNLADHAAARGPRLTMEEWTGHIDYAAWVLRILYEDEQVSRPIRLITGDDL
                     MSELDIHPGPLIGTLLDTIMEAQAAGEVGTREEALTLARATAASLSATRQGG"
     gene            complement(2058..2621)
                     /gene="def"
                     /locus_tag="VIM45_01825"
     CDS             complement(2058..2621)
                     /gene="def"
                     /locus_tag="VIM45_01825"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEY8820058.1"
                     /translation="MPIVVLGGPGDDVLRRPSNRVRDVRDRSIQRLIDDMFETMRDAP
                     GVGLAASQVGTPLRLIVVEETDPEHPAFALANPEIVKTSGRRRVTEGCLSIPGYQGEL
                     YRSETVVAKGLDRRGKEMRIKAPKGSLLAQALEHEIGHINGNLYIDWLEKREDLYRIR
                     QGSRESGDASDEGDDSVAAGGSREAAE"
     gene            complement(2724..4982)
                     /gene="priA"
                     /locus_tag="VIM45_01830"
     CDS             complement(2724..4982)
                     /gene="priA"
                     /locus_tag="VIM45_01830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEY8820059.1"
                     /translation="MPYAEVAISTRVPFAQAFSYAVSDGLVLSPGDAVLAPFGARLLP
                     GVVVSMGDRPAFDGELRTIETRLGDESLIDTHHIALARWLADRYLAPFPTALSLMLPR
                     GARDPADFLPPVPPAMHALRLEISAESALKSLAFAAPAIAGRALRSVAALINAGGIVP
                     IAELRSSHLLTNAVEKLLVSTGIVCAVETAHITAKPEPRLQRAEPAAVLTDDQQRAVA
                     AIELSMRRRFAGHDAPGSFLLHGVTGSGKTEVYLAALDIARRYGRQGIVLVPEIALTP
                     QTLERFSRRFPGRVAVVHGRLTRARHRAQWFGARAGEFDVVIGARSAIFAPLQHIGLI
                     VMDEEHEPSYKQSDPAPRYHAREVAAELARLTDAVLVLGSATPDAGSYRRAMDGEHRL
                     LALPKRVAGVADGTAGERPLPSLTVVDMARELREGHQRVLSRPLEADIEAALAAGEQT
                     LLFLNRRGSASLLLCRECGYTPRCPRCSVAYALHASEGKLVCHLCHHSRKAFERCPKC
                     RGARIRPVGIGTQRLEELVRHRFPEARVVRWDSDTANTSTRHAELARLIAHHEADIVV
                     GTQMVAKGHDFPHMTVVGVVSADLSLNVPDFRAAERTFQLLVQVAGRAGRRDVPGKVV
                     VQTYSPRHYAIALAAEQNYAGFMNRELGFRRQLSYPPFGALSRLVYAHTRPELAERGA
                     KRYGDLLRSERERLGLPGPQVIGPAPCYFSKVGGRWRWQVLLLGPGAHELLSAVTPPA
                     QWSIDVEPGELL"
     gene            complement(5034..5384)
                     /gene="rplS"
                     /locus_tag="VIM45_01835"
     CDS             complement(5034..5384)
                     /gene="rplS"
                     /locus_tag="VIM45_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEY8820060.1"
                     /translation="MDLSSYLELENNPNVPDFNTGDTVKVSSKVVEGDRERTQVFEGV
                     VIRRRNAGSSTNFTVRRIAHGVGVERTFLVHSPRVEKVEVVRQGKVRRSRLYYLRGLT
                     GKAARIKERARPRR"
     gene            complement(5530..6252)
                     /gene="trmD"
                     /locus_tag="VIM45_01840"
                     /pseudo
     CDS             complement(5530..6252)
                     /gene="trmD"
                     /locus_tag="VIM45_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256255.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
     gene            complement(6261..6773)
                     /locus_tag="VIM45_01845"
     CDS             complement(6261..6773)
                     /locus_tag="VIM45_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015049333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HEY8820061.1"
                     /translation="MRVGVLTISDKGSRGERIDSSGAAIRELLGQAGSVVVRYEIVPD
                     ERDLIAARLLQWVDGAEIDVVLTTGGTGLSSRDVTPEATLTVLDYEVPGIMEALRAEG
                     LKHTPMSMLSRAVAGVRGRTLIVNLPGSERAVRQNLNVLLAVLPHAVETLRDRSGNHV
                     ASDASTTSGE"
     gene            7104..>7349
                     /gene="mraW"
                     /locus_tag="VIM45_01850"
     CDS             7104..>7349
                     /gene="mraW"
                     /locus_tag="VIM45_01850"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012624735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytosine(1402)-N(4))-methyltransferase"
                     /protein_id="HEY8820062.1"
                     /translation="MTDPAGNHVAVLLDETIAALAPHTGGRYLDGTVGLGGHAERILD
                     ESSPNGSLLGLDRDHEALSLARVRLQRFGARAALVHAN"
BASE COUNT         1363 a         2415 c         2255 g         1316 t
ORIGIN      
        1 tcgctgggcc agaccacgta caccgcaaat gcaaacagca ctacgacggc cgctagcagg
       61 attcgattcg cgttttcgcg catgttgcga ctggacctcg acaggcgccc gcctctacag
      121 gttatgcggg cgcaccagaa atttttacac catttcaagt agtatcgcgc cacgggggag
      181 ctaggccaag tggggaggaa gacgtgaccc tctatctccc cagcggttag ggggctgcgg
      241 gaactggttc gggttgggtt cggcgttcga ggatcgcggt ggtaagcgcc cgcacttcca
      301 tccccggggc aagctccaat acgagctcga tcggacctac agcaggctgg cggatatcga
      361 tgaccgtggc gatcaatccg ccggtagtta cgacctcgtc gccagtttgc agctcgagca
      421 gatcgcgttt agtcttgcgc tgctgctgca gcacagggcg tagcaagatg tagtagaagc
      481 cgccaagcaa gatggccgac gtaaccatca actgcagcat gatcgccatc ggattaccct
      541 ccttgccgtg tcgccgacaa tgaagcggcc gttgctcgtg ccagcgtcag cgcttcctcg
      601 cgcgtcccga cttccccggc ggcctgcgct tccataattg tatccagcag tgtcccaatc
      661 agtggacccg ggtgtatatc cagttcgctc atcaggtcgt cgccggtaat gagccgaatt
      721 ggtcggctaa cctgctcatc ctcatatagg atgcgcagta cccaggcggc atagtcaatg
      781 tgcccggtcc actcttccat cgtcagacgc ggaccccgag ccgccgcatg gtctgccagg
      841 tttaacagca gcaggtccgg cgatgcgtca cccaggtctc tgaaaaaacg aaacagcgca
      901 cgccgcgtcg gcgcctcgcc agggcccgcc agcattcccg gccttaggtg ctgggcgatc
      961 agcaagccgg cgtactggat ctcgtgcgca gaaaggcgca gcgctcccag cgtgtccatc
     1021 gtacggcggg cgcccagctc aggatggcca tagaagcgca tattaccgga ttgcgaatcg
     1081 accgtcttcg tttcgggctt actgatgtcg tgcagcagtc cggcgacttt caacattgac
     1141 cgacgcgtcc gcccctcgcc cagttgcgct tccagccgct gaggaagtcc cggaacccaa
     1201 tcgaagccat cccataacgc cctccagagc gttgcgtcga ccttgtgcgt tggtggcgcg
     1261 cgtcgcagca tcaggtcaag cgccgccagc gtgtgcaggc tgtgctcgaa cacgtcgtag
     1321 tagtgctctt ttggctggga gacagagcgc gtcgcttcca ggtccggcaa catggtcgtg
     1381 agcagacgca ggctatcaag cgtgcgaata ccgctggccg cgttgtcgca ctcgaacagg
     1441 cgcataaatt cgtcgcgtcg acgctcggcc gcggcgcgcg gcaggagaga cgccgcggcg
     1501 cgagcgagtt cctgcgtctg ctggtcgagg gtgaatccga gctcgaggcc aaaccgcacc
     1561 accctcagca ggcgtacggg gtcgtctatg aaggactcgg gtgaagcgac cttcacccgc
     1621 ttgcgcctga gatcccccag tccgcccgtc ggatcgatga aagagggacc ttcaccgtca
     1681 accggcacag cgatcgcgtt gatagtgaag tcgcgcgccg ccagatctcc ttccagagtg
     1741 ggggcgcgga gccgcgtcag gtccacggtc cgcatctgtt ccattggctc gcgaagaact
     1801 acgcggtagg tccctcgatc ggggtcgagc gccacgactg ccccgttggc tcgggcacca
     1861 attcgtcgcg ccatgctcaa cgggtccccg tgcacggcca agtcgaggtc gtggttctgg
     1921 cgccccagca acgcgtctcg aacgaatccg ccgaccaaat aggcgcgcgt gtcctcactg
     1981 gccgccagaa gcgacaagag ttccggtggg atcagttgaa tacactcggc cgcgctaacc
     2041 acggaagagc ttcccgtcta ctcggctgcc tcgcgggaac ctcccgcagc taccgagtca
     2101 tcgccttcat cgctcgcgtc tccgctttct cgggacccct gacggatgcg atacagatcc
     2161 tcgcgctttt cgagccaatc gatgtagagg ttgccgttga tgtgaccgat ctcgtgttcg
     2221 agcgcttgag ccagcaggga acccttcggc gcctttattc gcatctcttt cccgcggcgg
     2281 tcgaggccct tcgcgacgac ggtttcggat ctatagagct cgccctggta cccgggtatc
     2341 gacaggcagc cctcggtgac acgccttcgc ccgctcgtct tgacgatctc cggattggca
     2401 agcgcaaacg ccgggtgctc gggatcggtc tcctctacca caatcagccg cagcggcgtg
     2461 ccaacctgcg acgcggccaa tccgacgccc ggcgcatcac gcatggtctc gaacatgtcg
     2521 tcgatcagcc gttggatgga gcgatcacgc acgtcccgga cgcgattgct cgggcgccgc
     2581 aagacgtcat cgcccgggcc cccgagcact acgataggaa taatcgccac tcgctcgctc
     2641 cgctcttcgc cggctcggtc tggatccgac acgcaccatt gtaaaggatg ctttgcagcc
     2701 gccaaataca atcggagcgc cggctagagc aactcacccg gttccacgtc aatcgaccac
     2761 tgtgccggcg gcgtgaccgc actgagcaat tcgtgtgccc cggggccgag taacaacacc
     2821 tgccaccgcc agcgaccgcc aactttgctg aagtaacacg gcgccggacc gatcacctgc
     2881 ggaccaggca accccaaccg ctcgcgttcg gatcgcaaga gatcaccgta gcgtttggcg
     2941 ccgcgctcgg ccaactcggg cctggtgtgg gcatatacca acctcgatag cgcgccgaac
     3001 ggcgggtacg agagctggcg acggaagccg agctcgcggt tcataaagcc ggcgtagttc
     3061 tgttcggcag ccagcgcgat agcgtagtgc cgcgggctgt acgtctgtac cacgaccttt
     3121 ccaggaacgt cgcggcgacc ggcgcgaccg gccacctgca cgagcagctg aaacgtccgt
     3181 tcagccgcgc gaaagtccgg gacgtttagc gagaggtcgg cgctaaccac gcctaccacc
     3241 gtcatgtgcg ggaagtcgtg gcctttggcc accatctggg tccccaccac gatgtcggct
     3301 tcgtgatggg caatgagacg ggcgagctcc gcgtgccttg tcgaggtgtt cgccgtgtcg
     3361 ctgtcccagc ggacgacccg cgcctcggga aatcggtgac gcacgagctc ctcgagccgc
     3421 tgcgtgccga tgccgacggg acggatgcgc gcgccgcgac atttggggca gcgttcaaag
     3481 gctttgcggc tgtgatgaca caggtggcaa acaagttttc cttcggatgc atgtagcgcg
     3541 taggcgaccg agcaacgcgg acagcgcggc gtatagccac attcgcggca gagcaaaagc
     3601 gaggcgcttc cgcgccggtt gaggaacagc aacgtctgtt caccggcagc gagagccgcc
     3661 tcgatatcgg cctccagtgg gcggcttagc acccgctgat ggccttcacg cagctcgcgg
     3721 gccatgtcta ccacagtcag cgatggcagc gggcgctcac cggccgttcc gtctgctacg
     3781 cctgctaccc gctttggcaa cgccagcagc cggtgttcgc cgtccatcgc gcggcggtag
     3841 ctgcctgcat ccggagtcgc cgagcccaga acgagcaccg cgtcggtgag gcgagccagt
     3901 tctgcggcga cctcgcgcgc atggtaccgt ggcgccgggt ccgattgttt gtatgaaggt
     3961 tcgtgctcct cgtccatgac gatcagcccg atgtgttgca gcggcgcgaa tatggcactg
     4021 cgtgcaccga tgacgacgtc gaactcaccc gctcttgctc caaaccactg tgcacggtgg
     4081 cgggcgcgcg ttagcctccc atgaacgaca gcaacgcggc cggggaaccg cctggagaac
     4141 ctttcgaggg tttgcggcgt tagcgcgatt tcgggcacca aaacgatccc ttgacggccg
     4201 taccggcgcg caatatccag ggcggccagg tagacctccg tcttaccgct gccggtcact
     4261 ccgtgcaaca agaaggatcc cggggcgtcg tgccccgcga agcgccgccg catcgatagc
     4321 tcgatcgccg caaccgcacg ctgctggtca tcagtcagaa cagccgccgg ctcggcacgc
     4381 tggagccgag gttcgggctt cgccgtaatg tgtgccgtct cgacggcgca aacgatcccg
     4441 gtggagacga gtagcttctc tacggcattc gtgaggagat gggagctccg aagctcggcg
     4501 attggaacga tgcccccggc attgatgagc gcggcaaccg agcgcagcgc gcggccggca
     4561 atggcaggtg cggcgaaggc gagggatttt aacgctgatt ccgccgaaat ctccagccga
     4621 agagcgtgca tcgccggcgg taccggcggt aggaagtccg ccggatcacg tgcaccacgt
     4681 ggaagcatca gcgacagcgc ggttgggaac ggagcgagat agcgatcggc cagccatcgg
     4741 gcaagcgcaa tgtggtgcgt gtcgatgagc gattcgtcgc caaggcgggt ttcgatcgtg
     4801 cgcagctcgc cgtcaaacgc aggccggtcg cccatcgaaa cgacgacgcc cggcaacaac
     4861 cgcgctccaa aaggggccag caccgcatca ccgggactaa gcacgagccc gtcggaaacg
     4921 gcgtagctaa acgcctgtgc gaacggcaca cgcgtgctga ttgctacttc tgcatacggc
     4981 aaaggcgggg ccacctggtg accggccgca tggcgccggt cggtgcagcg agactatcgg
     5041 cgcgggcgag cccgctcctt gatgcgcgcg gccttaccgg taagtccccg caggtaatag
     5101 agacgcgaac gtcgcacctt accctgccgg acgacttcca ccttctcgac gcgcggagag
     5161 tgaaccagga acgtgcgttc gacacccaca ccgtgagcga tgcgccgcac ggtaaagttc
     5221 gtgctgctgc cggcgttccg gcggcggatg accacgcctt cgaatacctg cgtccgctcg
     5281 cgatcgcctt cgacgacttt cgagcttacc tttacggtat cgccggtgtt gaagtccggc
     5341 acgttcgggt tattttccag ctcgaggtag ctgctcaggt ccatttgtgg tttctcccct
     5401 gtaccctcgc atcggcgtcc gcgccggccg cgggccgtcg gagtgacctg tctgctcaga
     5461 atccggttgc tggtctagct cgtgtgaatg cgcatcgtgt cgatgatcgc cggccgcggt
     5521 gggttcgagc cccagttccg ccaaccaccc ctgctcggac ggcgtaagca cggcacgcgc
     5581 cagcaggtcg ggccgtcgcc gcgcagttcg ttcgacggag agtcggcgac gccaacgttc
     5641 gatctcggcg tggtggccgc tcaaaagtgt agcaggaaca ctccacccgc ggaagtccgg
     5701 gggccgcgta tactgcggat actccaacag gccctggcta tgtgattctt ccgtcaacga
     5761 ctcggcagcc ccgagcgcgc cggggagccg gcgcacaaca gcatctgtca cgatgattgc
     5821 cgctggttcg ccacccgaca aaacgtaatc gccgatgctg atctcatccg tggccaggta
     5881 ctcgcgcacg cgttcgtcga cgccctcgta gtgaccgcag atcatcatga ggcgctcttc
     5941 gccggccaac tgctcgacga taggctgcga cagcaccctc ccctgcgggc tgagcaatat
     6001 cacagggccg cgccgcgcat cctgctctgc aaccatctcg gccgcggcaa atatcggctc
     6061 cggcttcatc accatgcccg gtccaccgcc gtaggcgtaa tcgtcgcaaa cacgatgccg
     6121 tccgatgcca tggtcgcgga tgtcgtgcac gtgaatctca acgaggccgc tggcgaccgc
     6181 tctaccgata atgctttctg caaaaggacc gctaaacatg ccgggaaaga gggtgaagat
     6241 atcgatgcgc acggaagcaa ttactccccg gacgtcgtgc tcgcgtcaga cgcgacgtgg
     6301 ttaccgctgc ggtcgcggag cgtctccacc gcgtgtggga gtacggccaa taagacgttc
     6361 aggttctgac gtacagcgcg ctcgctcccc ggcagattga cgatcagggt gcgtccacgt
     6421 acgccggcca cggcccggct cagcatggac atcggcgtat gtttcaatcc ttcggcccgg
     6481 agcgcctcca tgatgcccgg cacctcgtaa tcgagcacgg tcagagtcgc ttcaggcgtc
     6541 acgtcgcggc tggaaagccc cgttccgccc gtggtgagca cgacgtcgat ctccgcgcca
     6601 tccacccact ggagcaaccg cgccgcgatc agatcacgtt cgtcgggaac gatttcgtaa
     6661 cgcacgacca cgctaccagc ctgacccaga agctctcgaa ttgcagcccc gctgctatcg
     6721 atacgctctc cacgcgatcc cttgtcgctg atcgttagca cacccactcg catacccgcc
     6781 aattgactcg ctccatccgt tcggaccgtg cgtgcggtat agcaacacct atggtccggc
     6841 actagattga cggtaataac attgacatgc ggcagggcca atgggagaat ggagaccgtc
     6901 cgggagcaat agaatcaagc tcaatccgtt atctaatcac aggtactgca aatgtgtccc
     6961 ggggaggatg ccccaattcg ccattcgaac caccggcagc taagaccatc agtgggtccc
     7021 cgccccggtg gcctcgcgct gcctccgagt atacatgagc ctcgagcctc gctttccggc
     7081 gggcccgggc gacttggaac gctatgaccg atccggctgg caaccacgta gcagttttgc
     7141 ttgatgaaac gatcgcagcg ctggcgcctc acacgggtgg ccgctacctg gatggcacgg
     7201 tcggcttggg cggacacgcc gagcgcatcc tcgacgaaag cagcccaaac ggcagcctgc
     7261 tcggtctcga tcgcgaccac gaggccctgt cgcttgcgcg ggtgcggctt cagcggttcg
     7321 gtgcccgagc tgcgctggtc cacgcgaat
//
LOCUS       DATCPE010000060         4654 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U7219
            k141_366753, whole genome shotgun sequence.
ACCESSION   DATCPE010000060 DATCPE010000000
VERSION     DATCPE010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955033
            Sequence Read Archive: SRR1586279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 4654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 16:49:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 640
            CDSs (total)                      :: 631
            Genes (coding)                    :: 627
            CDSs (with protein)               :: 627
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4654
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_366753"
                     /isolate="SMAG_U7219"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            78..1184
                     /locus_tag="VIM45_01855"
     CDS             78..1184
                     /locus_tag="VIM45_01855"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011929249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEY8820063.1"
                     /translation="MRNANQQQAALQLVETPVTAVREANGTGTSVPSGAVEGPRPRTG
                     ASTDGARWRHHHVLDLDDFGRDEISLVLETTDAMREVLARDIPRVPALRGTTVATLFY
                     EASTRTRASFELAGKVLGADVINVSSSGSSVEKGESLIDTVRTLQALGVDAVVIRHQS
                     SGAPYLAAAHTSASVINGGDGWHAHPTQALLDLYTMRTHLGDLRGRRVVIVGDISHSR
                     VARSDIWGLTALGAEVILCAPPTLLPAGLGRGVSTNQEEVALPRVTVEYEIERALKDA
                     DVVMTLRLQEERQQAGLLPSLREYSRRFEITRDRMALAAPHALVMHPGPMNEGVEIAP
                     DVAYSAQSHIEEQVTNGVAVRMALLYLIAREGSA"
     gene            1181..2530
                     /locus_tag="VIM45_01860"
     CDS             1181..2530
                     /locus_tag="VIM45_01860"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEY8820064.1"
                     /translation="MNATGSLLVRGARVIDPARGVDNVQDVLFADGRVVPAGSVTPDH
                     TIDATGLILAPGFVDLHCHLREPGYEYKENIRSGLDAAAHGGFTTVCAMPNTEPATDN
                     RSVVEFVLRTAKFVGLARVLPIGAVTVARAGKQLAELGELADAGCIGFSDDGNPVADS
                     EIMRRALEYATGLGLPIIDHCEDPSLARGGVMNEGWVSTRLGLKGQPAAAEEAMAARD
                     IQLAELTGGHVHIAHVSTAGTVELVRSAKARGVHVTAEVTPHHLTLSDEAVLYSDSGP
                     QVPAYDTNARVNPPLRGASDRDASVLGLADGTIDCVATDHAPHATTDKVCEFDAAAPG
                     ISGLETAMALVLELVHEGRLSMARAVEALTAGPVRALRLERFVPGIGSLAAGAPADAV
                     LFDPDASWTVDPRAFVSFGKNTPFAGRRVRGRVIATVYNGTISYMDPVFDGSRPVRS"
     gene            2533..3627
                     /gene="carA"
                     /locus_tag="VIM45_01865"
     CDS             2533..3627
                     /gene="carA"
                     /locus_tag="VIM45_01865"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEY8820065.1"
                     /translation="MPEELAWLVLEDGSVFQGTQAGATVRGFGELVFNTSMTGYEEIL
                     TDPSYAGQLVTMTYPLIGNYGTNAEDIESRKIQAAGLVMREECDRPSHWRNTESLRDF
                     LCRRGVPAIAGIDTRALTRRLRSGGVLMAAIAGDESPNAALAWLQDQPHYGSTDLARE
                     VTTTEPYSWEPNGTVEGHVVVLDAGVKYNIMRSLNERGYRTTALPCTATAEEMLALKP
                     DGVVLSPGPGDPEALDYVVKNVSGLVGRTPILGICLGHQVLGQVFGASTYKLKFGHHG
                     GNHPVKDLATGSVYITSQNHGYSVDGDSLKGGAQVSHLNLNDYTCEGLTHRDYPIVSI
                     QYHSEAAPGPRDNMYLFDRFLDLVRSNKRG"
     gene            3650..4108
                     /locus_tag="VIM45_01870"
     CDS             3650..4108
                     /locus_tag="VIM45_01870"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8820066.1"
                     /translation="MTTLDATIRRASDADAVDIAAIISRLVTEPNPVGFREAQSSEQA
                     SRWLVRLGDQGCIFVAQVEEKIVGFGALDFNTEEPDTGTIGVWILPDFRRRGLATMLG
                     ESILDFAREAGYRRIRGRLPANNEPALSFLSGLGALVPLQNPEMRFELPL"
     gene            4173..>4654
                     /locus_tag="VIM45_01875"
     CDS             4173..>4654
                     /locus_tag="VIM45_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase large subunit"
                     /protein_id="HEY8820067.1"
                     /translation="MNKPSKVLIIGSGPIIIGQAAEFDYAGTQACKAMREEGVISVLV
                     NSNPATIMTDRGIADIVYIEPLRLDVLTRIIERERPDGLLPTLGGQTGLNLAVELADA
                     GVLDKYGVRVLGTPLKAIRQSEDRELFKRLLEELGEPVLPGETVTTIEAAKLVIAGIG
                     L"
BASE COUNT          876 a         1487 c         1495 g          796 t
ORIGIN      
        1 gacgaagatg ttcaggttct cctggacgag accgatggac gtgacgaggt gatgctgtgc
       61 cccggggaga atagctgatg cgtaacgcca accaacaaca ggcggcactg cagttggtcg
      121 agacgcctgt taccgccgtc cgcgaggcga acggtacggg cacatccgtc cccagcggtg
      181 cagttgaggg acctcggcca cgtaccggcg cttccactga cggcgcgcgc tggcgccacc
      241 accacgtcct tgacctcgac gatttcggcc gcgacgaaat ctcgttggtg ctggaaacga
      301 ccgacgcaat gcgtgaagtt ctcgctcggg acatccctcg ggtgccggcg ctgcgaggca
      361 ccaccgttgc caccctcttc tacgaagcaa gtacgcgtac gcgggcatcg ttcgagttgg
      421 ccggtaaagt cctcggcgcc gacgtcatca acgtgagctc gagcggcagc agcgtcgaga
      481 agggcgaatc gctgatcgat accgttcgca cgttgcaggc gctcggcgtg gacgccgtcg
      541 tcattcggca tcagagcagc ggcgcgccct atttagcggc cgcgcacacg agtgcctcag
      601 tcatcaacgg cggcgatggc tggcacgcgc acccgacaca ggcgttgttg gacctctata
      661 cgatgcgaac ccacctgggc gacctgcgcg gccggcgggt cgtcatcgtc ggcgacattt
      721 cgcacagtcg cgtcgctcgt tcggacatct gggggcttac cgccctgggc gcggaagtca
      781 tactctgcgc gccgcccaca ctgttgcctg ccgggctcgg ccgcggcgta agtacgaatc
      841 aggaagaagt tgcactgccc agggtcacgg tcgaatacga gatcgaacgt gcgctcaagg
      901 acgccgatgt ggtaatgacc cttcgcttgc aggaggagcg tcagcaggcg ggcctgctgc
      961 ccagcctgcg tgaatattct cgccgcttcg agatcacgcg cgaccgcatg gcgctggcgg
     1021 ctccacacgc gctggtcatg caccccggcc ccatgaacga aggcgtagaa atcgcgccgg
     1081 acgtcgcgta ctctgcccag tcacacattg aagagcaggt caccaacggc gtcgcggtgc
     1141 gcatggcgct cctctacctc atcgcgcggg agggctcggc atgaacgcca ccggctcctt
     1201 gctcgttcgc ggcgcccgcg tcatcgaccc tgcccgcggc gtcgacaacg ttcaggacgt
     1261 actttttgcc gatggccggg tcgtgccggc cggttcggtc acgcccgatc acaccatcga
     1321 cgccacgggg ttgatcctag cccccggctt cgtggatctg cactgccatc tgcgcgaacc
     1381 aggatacgag tacaaggaga acatccggag cggcctcgac gcggccgcac acggtggatt
     1441 taccaccgtt tgcgcgatgc cgaatacgga acccgcaaca gacaaccgct ccgtcgtcga
     1501 gttcgtgctc cgcacggcaa agtttgtcgg gctggcccgc gtgctcccga ttggcgcggt
     1561 cacggtcgcc cgtgccggca agcagctcgc ggagcttggg gaactggcag acgcgggctg
     1621 tatcggcttt agcgacgacg gcaaccccgt cgccgattcg gaaattatgc gccgcgccct
     1681 cgaatacgcc accgggctcg gcctgccgat tatcgaccat tgcgaagatc cgtcactggc
     1741 gcgcggcgga gtcatgaacg aaggctgggt ttcgacgcgg cttggtctca agggacagcc
     1801 cgccgccgcc gaagaagcaa tggcggcgcg cgacatccag cttgccgagc ttaccggtgg
     1861 ccacgtccac atcgcgcacg tcagcacagc gggaacggtg gagctcgttc gcagcgcaaa
     1921 ggcgcgcggc gttcacgtga ccgccgaagt gacgcctcac cacctcacgc tcagcgacga
     1981 ggcggtgtta tacagcgatt ctggcccgca agtgcccgcg tacgacacca acgcgcgcgt
     2041 gaatccgccg cttcgaggag catccgaccg ggacgccagc gtcctcgggc tggcggacgg
     2101 caccatcgac tgcgtagcca cggaccatgc gccgcacgcg accaccgaca aggtctgtga
     2161 attcgacgca gcggcgcccg gcatcagcgg tctggagaca gccatggcgc tcgtgctcga
     2221 gctggtccac gaaggcaggc tctcgatggc ccgagctgtc gaggcgctga ccgccggccc
     2281 ggtacgtgcc ctccgcctgg agcggttcgt gcctggcatc ggatcgttgg ctgccggcgc
     2341 acccgccgac gccgtactgt tcgatccaga cgcatcatgg accgtcgatc cccgagcctt
     2401 cgtgtcgttc ggtaagaaca cgccgttcgc cggtcgccgc gttcggggcc gggtgattgc
     2461 cacggtctac aatggtacga tttcctacat ggacccggta tttgacggta gtcgcccggt
     2521 gcggagctga cgttgccgga agaacttgcg tggctggtac tggaagatgg cagcgtcttt
     2581 cagggaaccc aggcgggagc aacggtcagg ggcttcggag agctcgtatt caacacatcc
     2641 atgaccggtt acgaggagat cttgaccgac ccgtcgtacg caggtcaact cgtgacgatg
     2701 acgtacccgc tgatcggcaa ctacggtacc aacgccgagg acattgagtc gcgcaagatc
     2761 caggccgccg ggttggtgat gcgggaggaa tgcgaccggc cgagccactg gcgcaacacc
     2821 gagtcgctcc gcgacttcct ttgccgccgg ggcgtgcctg caatcgctgg aatcgatacc
     2881 cgtgcgctaa cgcggcggct ccgttcgggc ggcgtgttga tggcggcgat tgccggcgat
     2941 gaatcgccca acgcggccct tgcctggttg caggaccaac cccactacgg cagcaccgat
     3001 ctggcgcgcg aggtcaccac tacggagccg tattcctggg agcccaatgg cactgtcgag
     3061 ggccacgtag tcgtcctcga cgcgggcgtc aaatacaaca tcatgcggtc gctcaatgaa
     3121 agaggctacc gtacgactgc gctaccatgc accgcaacgg ccgaggaaat gctcgcgctc
     3181 aaacccgacg gcgtcgtgct ctcaccgggc cccggcgatc cggaggcgct tgattatgtc
     3241 gtgaagaacg tcagcgggct ggtgggcagg acgcctatcc tgggaatttg cctcggacat
     3301 caagtgctcg ggcaggtatt cggtgcgagc acatacaagc tcaagtttgg gcatcacggc
     3361 ggcaatcacc cggtgaagga tctcgccacg gggagcgtct acattacgtc gcaaaaccac
     3421 ggctactcgg tagacggcga tagcctcaaa ggcggagcgc aggtcagcca cctcaatctg
     3481 aacgactaca cgtgtgaggg tctcacccac cgtgactacc cgatcgtctc gatccagtac
     3541 cattccgagg cggcgccggg tccgcgggac aacatgtacc tctttgaccg ttttctcgac
     3601 ctcgttcggt cgaacaagcg cggttgaaat cgatgaaagg cgacggccga tgacgacctt
     3661 agacgccacc atacggcggg caagcgacgc cgacgccgtc gatatcgcgg cgatcatcag
     3721 ccgcctggtc acagagccga atccggtcgg tttccgcgag gcgcaatcgt ccgagcaggc
     3781 ttcccgctgg ctggtcaggt tgggagacca gggctgcatc ttcgtcgcgc aggtagagga
     3841 gaagatcgtc ggctttggcg cgctggactt caacaccgag gagccggata ccggcacgat
     3901 cggcgtatgg atcctgcccg atttccggcg gcgaggtctg gcgacgatgc tcggtgagtc
     3961 gatcctcgac ttcgcccgcg aagccggata ccgccgcatc cgaggccgct tgcccgccaa
     4021 caacgagccc gcgttgtcat ttctgtcggg attgggcgcc ctggtgccac tccagaaccc
     4081 ggagatgcgc tttgaactgc cgctctaggg tcacggagcg acccctatcc cttaccgggg
     4141 aggatcccaa cggatggaga ttgtaaagac gcgtgaacaa gcccagcaaa gttctcatta
     4201 tcggctccgg gccaatcatc atcggccagg cggcggaatt cgattacgct ggcacgcagg
     4261 catgcaaggc tatgcgcgaa gagggcgtga tctcggtgct ggtcaactcc aacccggcga
     4321 cgatcatgac cgaccgggga attgcggaca tcgtatacat cgagccgctg aggctcgacg
     4381 tcttgactcg gatcatcgaa cgcgagcggc cagacgggct gctgccaacg ctaggaggcc
     4441 agacggggct gaacctggca gtagagcttg ccgatgccgg agtcctcgac aaatacggag
     4501 ttcgagtgct cggcacgccg ctgaaggcca tcaggcagtc cgaggaccgt gagctgttca
     4561 agcggttgct ggaggagctc ggtgagccag tgcttccagg cgaaacagtg accactatcg
     4621 aggcggcgaa gctggtcatc gcgggcattg gact
//
LOCUS       DATCPE010000061         4172 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U7219
            k141_399603, whole genome shotgun sequence.
ACCESSION   DATCPE010000061 DATCPE010000000
VERSION     DATCPE010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955033
            Sequence Read Archive: SRR1586279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 4172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 16:49:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 640
            CDSs (total)                      :: 631
            Genes (coding)                    :: 627
            CDSs (with protein)               :: 627
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4172
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_399603"
                     /isolate="SMAG_U7219"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1881
                     /locus_tag="VIM45_01880"
     CDS             <1..1881
                     /locus_tag="VIM45_01880"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY8820068.1"
                     /translation="YGAAILAAGAICAALWVRQRTGQGQLVEVSWLAGGFAVQTGTIL
                     MGEGITRLSGVPVNPLGPVPVYRVFRAADDRYLFLACGHSGFFHRFCLLIDRPDMISD
                     SRFQNAPWGIVDPDDRAALARELEPIMASKPRDEWLTLLSAADIPCAPVLLRDDYLQD
                     PQVEHIGMRAVVEDPELGSTVQPGTPLRLHGTPGGIRGGAPLLGGRLLDGTPFGAGDS
                     AAIRGETSPNVPLPASALAGLRVLDLSAYIAGSLCPMTLADWGADVIKIEGLDGDPFR
                     AFGFGFLGWNRGKRSVAVDLKRPEGRELIYELVRGADVVVENFRPGVSERLGVDYETL
                     QAINPRLIYSSETAYGYSGPYVTRPGFDPLLQARSGMMAAQGGLLDGHSPVFLTVALC
                     DYGAALLSVFGISAALVARERTGEGQRVETNLTSSAMAMQMAEFVRYQGSPPPLEGGA
                     DYRGPTALCRAYEAQDGWLLLAIRDATHWVPLTQALDRFDLLHTYTPLEAIEETGHGH
                     LATTIATAFLTRTVAEWLDRCDRAGVPAGPVISAQALFSDPHIAANDLIVTHQHPVWG
                     RVEQTGQLVKFSRTPGKIGPVASMLGQHTEEVLIEAGIGRDRVRTLLLGGIISQHQGN
                     EA"
     gene            complement(1892..3082)
                     /locus_tag="VIM45_01885"
     CDS             complement(1892..3082)
                     /locus_tag="VIM45_01885"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEY8820069.1"
                     /translation="MAVSVVRKRLRTAMVGGALLVVLYVLYAARGVLPPFLLGIVFVI
                     VCGPIVDRLAHDLPFRRRHPDLALSVSIALVYFVLAGIFALIVVLIGPRLLAEGKNLA
                     SVLPSLVQRAQRELQDNSGWYQSNVPADIRTQIDQNWQQIANKLGEYGQTIFQHTINF
                     ATDSVTTLISYIVVPFWAFFVLKDRAKATRWFLGLFPAEVQPDVEHLLLNAQSVFGFY
                     LRAQLLLATATGVVTTIGLVWFGVRFAVALGVIAGVANLIPVIGPMLGGIPALIVVLA
                     THPGWEVLWVFLFLFVAQELKDFLLVPRIQGRAVRIHPAFILLLIVVAGHIAGFWGLL
                     LVVPLAAVIRDTFVYIYRRLGQDPIVAVPTARTAASTAGLPPVEHDGMADGLPRRAAK
                     ALRG"
     gene            3316..3552
                     /locus_tag="VIM45_01890"
     CDS             3316..3552
                     /locus_tag="VIM45_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8820070.1"
                     /translation="MARIIVANRHGHQVVEYDTVTDTEEARRSVEAAERILREARDQG
                     CRVSKKVGDQHVIDDGPFDPTVEEYQVIAPLAGG"
     gene            3658..>4172
                     /locus_tag="VIM45_01895"
     CDS             3658..>4172
                     /locus_tag="VIM45_01895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8820071.1"
                     /translation="MSSSFFRRLVDEFLSLVTPSRRDEYLLTPGADAWAMDDGEPDED
                     EDWFGDALARHWRTGVNVDDARRLGYWPGQEHRPDFVRAWQSEGAQALERPWNYRWDR
                     LPKRSAGPGFSRGDYRDARVRALEIARETLGEDLWQQLELNGYLDVRSSKFPGVTYRL
                     RVGRRIQVLCDP"
BASE COUNT          808 a         1248 c         1359 g          757 t
ORIGIN      
        1 tacggcgcgg ccatcctcgc cgctggtgca atctgtgcgg cattgtgggt cagacaacga
       61 accggtcagg ggcaactggt tgaggtatcg tggctggcag gcggtttcgc ggttcaaacg
      121 ggtacgatcc tcatgggcga agggatcacc cggctttccg gcgtgccggt caaccccctc
      181 gggccggtgc ccgtttaccg ggtctttcgg gccgcggacg accggtatct ttttctcgcc
      241 tgcgggcaca gcggtttttt ccaccgtttc tgcctgctga tcgatcgtcc cgacatgata
      301 tcagattcac gcttccaaaa cgctccctgg ggcatcgtcg acccggacga tcgagcggcg
      361 ttggcgagag agctggaacc gatcatggca agtaagccgc gcgacgaatg gctcaccttg
      421 ctctccgcgg ccgatattcc ctgcgcaccc gtcctgctgc gcgacgatta cctgcaggac
      481 ccgcaggtcg agcacatcgg aatgcgcgct gttgtggaag atccggaact gggctcgacc
      541 gtccagcccg gaacgccgtt gcgtttgcat ggaaccccgg gtggtatccg cggtggcgct
      601 ccgttgctcg ggggtcgact gttggatggc acgccgttcg gcgccggtga ttccgcggcg
      661 attcggggcg aaacgtcacc aaatgtgccg ttgccggcga gcgccctggc agggctgcgc
      721 gttctcgacc tctcggccta catcgccggt tcgctctgcc cgatgacgct cgccgactgg
      781 ggcgccgacg tcatcaagat cgaggggctg gatggagacc cgttccgcgc gtttggcttc
      841 ggtttcctag gctggaaccg cggcaagcgc agcgttgcgg tcgatctgaa gcgcccggag
      901 ggtcgcgaac tgatctacga actggtacgc ggcgccgatg tggtcgtgga aaacttccgt
      961 ccgggtgttt cggaacgcct aggggtcgac tacgaaacgc ttcaggcgat caatccgcgc
     1021 ctaatctatt cgagcgagac tgcctacggt tacagtggtc cgtacgtgac ccgtcccggc
     1081 ttcgatccgc tgctgcaggc gcgatcgggg atgatggcag cgcagggagg cctccttgac
     1141 ggtcattccc cggtgttcct cacggtcgct ctttgtgact atggtgcggc attgctcagc
     1201 gtatttggca tcagcgccgc actggtggcg cgagaacgaa ccggcgaagg ccaacgggtg
     1261 gagaccaacc tcacgagctc ggcaatggct atgcaaatgg ctgagttcgt ccggtatcag
     1321 ggttcaccac cgcccttgga gggcggcgcc gattaccgcg gaccgactgc gctttgccgc
     1381 gcgtatgaag cgcaggacgg ctggttgctg ctggctatcc gtgacgccac gcactgggtg
     1441 ccgctaacgc aagcactcga tcggttcgac ctgttgcata cctacactcc actggaggcg
     1501 atcgaggaga cggggcacgg acacctagct acgacaattg ccacggcgtt cctcacaaga
     1561 actgtcgcgg aatggctcga ccgctgcgac cgagcgggtg tcccggccgg gccggtgatc
     1621 agtgcacagg cgctcttctc cgacccgcac attgccgcaa atgacctgat cgtgacgcac
     1681 cagcatccgg tttggggtcg cgtcgagcag accggccagc tggtaaagtt tagccggacg
     1741 cccggcaaga tcggacctgt tgcttcgatg cttggccagc acaccgaaga agtgttgatc
     1801 gaggccggca tcggccgaga tcgggtccgc actctactcc taggcggcat catttcgcag
     1861 catcaaggga acgaggcctg agccggctga gtcagccgcg gagcgccttc gccgcgcggc
     1921 gcggcaggcc gtccgccatg ccgtcatgct caacgggcgg tagtccggcc gtcgaagccg
     1981 ccgttcgcgc cgtggggaca gcaacaatcg ggtcttgtcc gagcctccgg tagatgtaga
     2041 cgaaggtgtc ccgaatgacg gcggccaggg gcaccaccaa cagcaatccc cagaacccgg
     2101 caatgtgtcc ggcgacgacg atgagcaaca gaatgaacgc cgggtggatg cgcacagcgc
     2161 gaccctggat gcgcggcacg agcagaaagt ccttcagttc ctgggcaacg aagaggaaga
     2221 gaaacaccca cagaacttcc cagccgggat gggtggcgag caccacgatc agcgcgggga
     2281 tgccgccaag catcggacca attaccggaa tcaggttggc gaccccggcg atgacaccca
     2341 gcgcgactgc gaaacggacg ccgaaccata cgagcccaat tgtcgtcaca acgcccgtcg
     2401 ctgtagcgag gaggagctgc gcccgcaggt aaaagccgaa cacgctttgg gcgttgagaa
     2461 gcaggtgctc gacatctggc tggacttcag ccggaaatag cccgagaaac caacgggtcg
     2521 cctttgcgcg gtctttgagc acaaagaagg cccaaaaggg aaccacgatg tagctgatca
     2581 atgtggtgac gctgtcggtc gcaaaattga ttgtgtgctg aaagatcgtc tggccgtact
     2641 caccgagctt gttggcgatc tgttgccagt tctggtcgat ctgagtgcgg atgtccgcgg
     2701 gaacattgct ctggtaccag ccgctattgt cctgcagttc acgctgcgcg cgctgtacga
     2761 gtgaaggcaa cacgctggcg aggttcttac cttcggccag gagccgaggc ccgatcagga
     2821 caacgatcag cgcgaatatt ccggccagga cgaagtaaac gagcgcgatg ctcaccgaga
     2881 gggcgaggtc cgggtgccgg cggcggaacg gcaaatcatg cgcgaggcga tcgacgattg
     2941 ggccgcacac gatgacgaat acgatgccaa gcaagaacgg cggcaatacg ccgcgcgccg
     3001 cgtacagcac gtacaggacg accagcagag ccccaccgac catggcggtg cgcaggcgct
     3061 tccggacgac actaactgcc atcgtaacga cctcccggcg ccgaaattct cggctttgaa
     3121 cggtggcgag tcaagttggg tccgccagta tcctcgtaat ccatcgtgta agaccgtcta
     3181 aagatgcatg aagttgtaat gtcgcggagg ttggcaggca tcccaactgg acctaggata
     3241 cagggtaggg atgggcaggc cggtgcagcc catccggtga ggttccactg cgccgagagg
     3301 ttttggaggg gaattgtggc acggatcatc gttgcaaacc gccatggaca ccaggtagtc
     3361 gagtacgaca ctgtcacaga cacggaagag gcccgacggt cggttgaagc agcggagcga
     3421 atcctccgtg aagcgcgaga ccagggctgt cgggtatcga agaaagtcgg cgaccagcac
     3481 gtgatcgacg acggaccatt cgatccgacc gttgaggagt accaggtcat cgccccgctg
     3541 gcaggcgggt aggcggccca accgcaccac aactcgataa actgatggcc gccggcgacg
     3601 gaagcggtcg gattttctcg cctccgtgat cccggcttgc cttctggtaa taccggcgtg
     3661 tcatcttcat tctttcgccg gcttgtagat gagttcttga gcctggttac gccctctcgc
     3721 cgggacgaat acttgttgac gcccggtgca gatgcctggg cgatggatga cggcgagccg
     3781 gatgaggacg aagactggtt tggagacgcc ttggccagac actggcgcac cggcgtgaac
     3841 gtcgatgatg cgcgacggct tggttattgg ccgggacagg agcatcgccc cgatttcgtc
     3901 cgtgcctggc aatccgaagg cgcccaggcg ctggaacgac cctggaacta ccgctgggac
     3961 cggctgccga agcgcagcgc gggtcccgga ttttcgcgtg gagactaccg cgatgcgcgg
     4021 gttcgcgcac tggaaatagc ccgcgagacg ctcggtgagg atctctggca gcagctcgaa
     4081 ttaaacggct atctcgacgt tcgatcgtcc aaatttccgg gggttacgta tcgcctccgg
     4141 gtcgggcgcc gcatccaggt gctctgtgat cc
//
LOCUS       DATCPE010000062        10662 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U7219
            k141_108036, whole genome shotgun sequence.
ACCESSION   DATCPE010000062 DATCPE010000000
VERSION     DATCPE010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955033
            Sequence Read Archive: SRR1586279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 10662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 16:49:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 640
            CDSs (total)                      :: 631
            Genes (coding)                    :: 627
            CDSs (with protein)               :: 627
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10662
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108036"
                     /isolate="SMAG_U7219"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..954)
                     /locus_tag="VIM45_01900"
     CDS             complement(<1..954)
                     /locus_tag="VIM45_01900"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEY8820072.1"
                     /translation="MIEQRLNRLRAQLDEHELDALFVSDPFNRRYLSGFTGSNGWLLV
                     SRDEAVLATDFRYWEQAGSQSPHFRLFKTVGPMPDWLGELFDGLRGKRLGFEQAHLTY
                     GDYRAVVGIIEGFEPASRPKFMPALPIVEQLRSIKDACEMIAMERVIALGDAAFEHVA
                     ARIEPGWTELQASRAIENYVKDNGGEGTSFTSIVAGGARGAMPHARAGRDVLREGEPV
                     VIDMGALLDGYCSDMTRTIFLGNPTNRFNVIFDIVFTAQRTAEELIEPGMTGDYAHKL
                     AQRVIEEAGYGEHFGHGLGHGIGLQVHEAPRLAPKSTSVLQE"
     gene            complement(951..1397)
                     /gene="aroQ"
                     /locus_tag="VIM45_01905"
     CDS             complement(951..1397)
                     /gene="aroQ"
                     /locus_tag="VIM45_01905"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003394103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HEY8820073.1"
                     /translation="MRILLINGPNLNLLGARRPEIYGRATLAEIEAEARDRATAAGVE
                     LICYQSNSEGALIDFIQEHAGGADGIIMNPGAFTHYSYALRDAVEATELPLIEVHLSN
                     IHARESFRHTSVIAPVAVGQVAGLGRHGYPAALDALIAIVKEKRKA"
     gene            complement(1369..2346)
                     /gene="xerA"
                     /locus_tag="VIM45_01910"
     CDS             complement(1369..2346)
                     /gene="xerA"
                     /locus_tag="VIM45_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015407024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase/integron
                     integrase"
                     /protein_id="HEY8820074.1"
                     /translation="MHPLIQRYLDALSAEHRRSALTLRNYLTDLRAFDEYLRSTGRCD
                     LKSVDRVTFRGYLASLAERGVARGSVARIVSTIHTFYRHLLVLGSLSRDPLVGVRPPK
                     RERRLPRVLAEEEAVALVSSPIPDDAFGLRDRAILELLYASGVRVAEIVSLNVEAIDL
                     DEARMVVRGKGKKERVALFGNPAAEAIARYLYTGRPALTGKRGEPALFLNRDGGRLSA
                     RAVQTIVRQAGIASGIDAVTHPHMLRHSFATHLLDGGADLRVVQELLGHASASTTQIY
                     THVTESRQRQVYDEAFYNAWRSDKRKRRSSPPTSSAGGEGNANPAHQRP"
     gene            complement(2391..4667)
                     /gene="topA"
                     /locus_tag="VIM45_01915"
     CDS             complement(2391..4667)
                     /gene="topA"
                     /locus_tag="VIM45_01915"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEY8820075.1"
                     /translation="MPQAKQAKKNLVIVESPAKAKTVASILGAGYDVRASVGHVRDLP
                     KSRLGVDEKRDFAPHYIIPKDKADVVNQIKRAAKNAQSVFLATDPDREGEAISWHLVQ
                     AADLENTPHKRVVFHEITRDAITDAFKHPRDIDMPLVNAQQARRVLDRLVGYKISPLL
                     WNKVRRGTSAGRVQSVALRMIVDSEHEIEEFTAREYWSIEAELAQAAGAPESFRARLQ
                     GYAGKKRELELNNEQQTSAIIEQLRTSSYEVAEVRQRPQSRRPAAPFTTSTLQQEASR
                     RLGFTAKRTMTIAQQLYEGRSIGSEGEVGLITYMRTDSTVMSDVAKAEIRSYIADRFG
                     KQFMPEQARTYTKKAKGAQEAHEAVRPTGVLREPDRIKRFLTNDQYRLYQLIWQRAVA
                     SQMADALIDMTSVDIHAKPRGGDTFLLRATASHLRFPGFRAVYFERTEEGDDEDADTR
                     ALPDLKPGEALNLIDLFPEQHFTEPPARFTEATLVKALEENGIGRPSTYATILSNLMD
                     RTYVEKLGRALKPTQLGRDVNELLTSYFPGVVDLRFTAHMEEELDEIAAGNRAWSPVV
                     LEFFQPLKLALAEAENAPALMKPTDEICELCGRPMAIKWGRFGEFLACTGYPECTNSR
                     PLKKDEEDQAVNDELCPICNSGMVVKTGRFGRFLACERYPECKGTKTFLNKIGVNCPQ
                     CDGAVVERRTKGRRTFYGCSNYPRCDFVSWTRPTGEVCPNCSGAVVADGPNGVKCLKC
                     PWRNEREPEQEVVVEVPA"
     gene            complement(4724..5893)
                     /gene="dprA"
                     /locus_tag="VIM45_01920"
     CDS             complement(4724..5893)
                     /gene="dprA"
                     /locus_tag="VIM45_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011309191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HEY8820076.1"
                     /translation="MVDFRRRCYNPPSTNGPGGIQLEDTAYWLAFNRIPTIGRARFER
                     LERAFGTLERAWLASDADLRAAGIDDRSARAISAARPRISPERELAALQRIGVTALTW
                     NDPLYPPLLKESYDRPPVLYVRGTITTEDELALAVVGTRRMTAYGRQVTEHIAGDLAR
                     SGITIVSGLARGVDRTAHEAALGAGGRTIAVMACGVDIIYPAAHERLARQIVEHGALV
                     SELPLGTRPQKDLFPRRNRIIAGLTLGVVITEAPERSGALLTAKLAADDNREVFAVPG
                     SIFSPMSKGTNALIQEGAKLTGTAEDVLSELNLAAAAQQAELAAAVPATAVESTLLSC
                     LTAEPIHIDDVTRRSGLAAAEVSGGLALLELKGVVRQIGGMQYVRLRETAGEYTA"
     gene            5955..7187
                     /locus_tag="VIM45_01925"
     CDS             5955..7187
                     /locus_tag="VIM45_01925"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEY8820077.1"
                     /translation="MERNPWFRALLILGVAFLGVQLFLIVWHFGSHFAQTILIFFLAW
                     MLSFILNPAVDVVARRWRLGRPAAVSIVFLGIIAVVALVGFLVVPPAVKQVAALGDKV
                     PEYRQNTGKLVTDLQSWLDERHINIDITGVNTEDLSKQIDKTGASLAQNALGLAPKVI
                     EGIFDTVIILVVSFYMMLDAPRISAAMISVTPERYRRDVRMLFASIDHSFGGYIRASV
                     ALALLYAAGTALTMWGTGIPFVLPVSMFAGFMLIIPFIGDVIAVIPTIVIGLFTTSLV
                     HVIIALVAMIALQQLVLQVIRPKLMGKSVGLHPLWVLAAFFLGARAAGLWGALFSVPI
                     AAIIQSVVQLYYYRVTGRPQPAALAALMREATAPFPLRPEPNLPPEDESRDRPAADGT
                     PIPAATSGGPGRRGDDRD"
     gene            7235..8578
                     /locus_tag="VIM45_01930"
     CDS             7235..8578
                     /locus_tag="VIM45_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8820078.1"
                     /translation="MRVLVVGFPLPNPQFDNYTLLNSPSWFDYDAVIVEPESVSTVIE
                     NVVNRKEEHETRAEEPILNRPTTPFAVALADMIRRRRAEASQLLANGGVIAVFTRPNI
                     VHQEVVGFPGCDRYAWLPAPAGVSWDSPFLVRAEGTEVHASDSRHPFARMVDRHRPWF
                     FYRARIDENAPGFPTYGSVFARSYGGVAIGADLKVGAGRVILLPAFAPLAYGDQRFEL
                     ASEFLEALRLCTGQQADTKPPAWVSELSVPGSEELEASEREATEALAAAETRVAEAQA
                     SLTQLTKYRALIWQEGRFGLEPAVCDAFRAIGFEVESDPDKPGWIADGRVRAFYEVEA
                     STEAVLEYPYFRLQKRLEKDLIDTKEPKHGLIVVNGQRLSPPVNRNAQVSETLRIAVD
                     NYRYGLLTTERLFEIVKAVLADPLNAPLRSMIRSRLLNAVGEVGADVIPTAEELA"
     gene            complement(8579..8893)
                     /locus_tag="VIM45_01935"
     CDS             complement(8579..8893)
                     /locus_tag="VIM45_01935"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HEY8820079.1"
                     /translation="MTSLQHQVANWHIDRERLDEWESIWDQMHDVARQTPGFRTARLL
                     RSVEHPGKYTIYSLWDSRASWVEYYNTSKVQELIHSSFRLLKGPPVTEWFDLVREVHV
                     TG"
     gene            complement(8909..>10662)
                     /gene="lepA"
                     /locus_tag="VIM45_01940"
     CDS             complement(8909..>10662)
                     /gene="lepA"
                     /locus_tag="VIM45_01940"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HEY8820080.1"
                     /translation="LEVTGTLSAREMEEQVLDSMDLEREKGITIKAVAVRMFYTARDG
                     IEYELNLIDTPGHVDFTYEVSRSLAAAEGAILVVDAAQGIQAQTLANVYLALEHNLQI
                     IPVINKIDLPNAEPQRVMQELADVIGFDRDEMILASAKEGTGITDILEAVVKRVHPPR
                     GRAEAPARALIFDSKYDAYKGVVAYVRVVDGELNGRRPLKLMSSGLTVQPIEVGTFRP
                     GLLPGTELVTGEVGYVATGLKNVKECQVGDTFTDAARPAHEPLKGYQPAKPMVFAGIY
                     PQQANDYPLLRDALDKLQLNDASLVYEPESSMALGFGFRCGFLGVLHMEIVQERLERE
                     YGLELIATAPSVEYQVTLSDGKELNVDNPAELPPPEKVQEVREPWLNISVITPSRYIG
                     AVMELVTTRRGAFNKMEYLEHGDGAGSAAGEARVLLEYKIPLSEILVDFYDQLKSGTQ
                     GYASLDYNFSHYEAAKLVKLDVLVNGQPVDALSLIAHADRAPQQGRRLVEKLRSLIPR
                     QMFDVPIQAAIGGRVVARETVRAMRKNVLAKCYGGDITRKRKLLEKQSEGKKRMKRVG
                     NVEIPQEAFLAVLSLDD"
BASE COUNT         1889 a         3315 c         3264 g         2194 t
ORIGIN      
        1 ctcctgaagc acagaagtgg atttcggggc aaggcgcggt gcttcgtgga cctgaagtcc
       61 gatgccgtgc ccaaggccat ggccgaagtg ttcgccgtaa ccggcctctt cgatcactct
      121 ctgcgcaagc ttgtgggcgt agtcgccggt catgcccggc tcgatcagct cttcggcggt
      181 tcgctgagcg gtgaagacga tgtcaaagat gacattgaac cggttcgtcg gatttcccaa
      241 aaagatcgtg cgcgtcatgt ccgaacagta gccgtcgagc agagcgccca tatcgatgac
      301 gacgggttcc ccttcccgca gaacgtcgcg acccgcccgc gcatggggca tggctcctcg
      361 cgcaccaccc gcgacgatgg acgtgaaact ggtgccctcg ccgccgttat ctttgacgta
      421 gttctcgatc gcgcgtgagg cctggagctc cgtccagccg ggttcgattc tggcggcgac
      481 gtgttcaaac gcagcatcgc cgagcgcaat gacgcgctcc atcgcgatca tttcgcaggc
      541 gtctttgatc gaacgcaact gctcgacgat tggcagcgcg ggcataaact tcggtcgcga
      601 cgcgggctcg aatccttcga ttatgccgac cacggcgcgg tagtcgccgt aggtcaagtg
      661 cgcctgctcg aagccgaggc gcttgcctcg caatccgtcg aacagctcac cgagccagtc
      721 gggcatcggt ccgaccgtct tgaacaggcg aaagtgcggc gattggctcc ccgcctgttc
      781 ccaataccgg aagtcggtgg ctaggaccgc ctcatcgcgg cttacaagta gccagccgtt
      841 gctgccggtg aagccagaga ggtaacggcg gttgaatgga tccgagacga agagggcatc
      901 cagctcgtgt tcgtcgagct gcgcccggag ccggttaagg cgttgctcaa tcaagccttg
      961 cgcttctcct tgacgattgc gatcagcgca tcgagcgcag cggggtaacc gtgacggccg
     1021 aggcccgcga cctgtccgac tgcgaccggt gcgattaccg atgtatggcg aaacgattcg
     1081 cgagcgtgga tattcgaaag gtgcacttca atcaacggca gctcggtcgc ctcgaccgca
     1141 tcgcgcagcg cataactgta atgtgtgaac gcaccggggt tcatgatgat cccgtccgca
     1201 ccgccggcgt gctcctgtat gaagtctatc agagcgcctt cgctgtttga ctgatagcaa
     1261 attaattcga cgcccgcggc ggttgctcgg tcccgagcct cggcttcgat ttcggcgagg
     1321 gtggcacgtc cgtatatctc cggccgccga gcgccgagca ggttcaaatt agggccgttg
     1381 atgagcagga ttcgcattgc cctcgcctcc cgccgatgat gttggcggtg aagatcgccg
     1441 cttgcgctta tcggagcgcc atgcgttgta gaaggcctcg tcgtacacct gccgttgccg
     1501 gctctcggta acgtgtgtgt aaatttgcgt ggtgctggca ctcgcatggc ccaagagttc
     1561 ctggacgacg cgcagatcgg caccgccatc caacaaatgc gttgcgaacg aatgcctgag
     1621 catgtgcggg tgggtaacgg cgtcgattcc cgaggcaatc ccggcctgcc tgacgatggt
     1681 ctggaccgcg cgcgccgaca accggccgcc gtcgcgattc aggaacagcg cgggctcgcc
     1741 gcgtttcccc gtgagcgcgg gccggcctgt gtacagatag cgagcaatcg cttcggctgc
     1801 ggggttgccg aacagcgcga cgcgttcctt tttacctttt ccgcgcacca ccatgcgcgc
     1861 ttcatcgagg tcgatcgcct cgacgttcag cgaaacgatc tcggcgacgc ggactcccga
     1921 ggcgtacaaa agctccagaa ttgcccggtc gcgcaaaccg aaggcgtcgt cagggatcgg
     1981 agaagagacg agcgccactg cctcttcctc ggccagaacg cgcggcagac ggcgctcgcg
     2041 cttcggcggg cggacgccca ccagtggatc gcgtgatagc gatcccaaca ccagtaaatg
     2101 acggtaaaac gtgtggatgg tactgacgat gcgcgccacg ctgccgcgtg ctactccgcg
     2161 ttccgcgagt gacgccaggt agccacgaaa agtgacgcgg tccaccgatt tcagatcgca
     2221 acggccggta gagcggagat attcgtcgaa ggcgcgaagg tcagtgaggt agttgcgtag
     2281 cgtcagcgcc gaacggcggt gctccgcaga gagcgcgtcg aggtatctct ggatcagcgg
     2341 atgcacagga gctttcgccc gggacgcgtc cgctggctgc cgggcggcgg ctatgcgggc
     2401 acctcgacga caacctcttg ctccggctcg cgctcgttcc gccatgggca cttgaggcac
     2461 ttgactccgt tcggtccgtc ggcgacaacc gcgcccgagc agtttgggca gacttcgccc
     2521 gtcgggcgcg tccagctgac gaagtcgcat ctcgggtagt tgctgcaccc gtaaaacgtt
     2581 cgccgtccct ttgtccggcg ctccacgacg gcgccgtcgc actgcgggca atttacgccg
     2641 atcttattga ggaaggtctt tgtgcctttg cactccggat aacgctcgca ggccaggaag
     2701 cgaccgaagc ggccggtctt gacgaccatg cccgaattgc agattgggca gagctcgtcg
     2761 ttgaccgcct gatcttcctc gtcttttttc agtggccggc tattagtgca ctccgggtag
     2821 cccgtgcagg cgagaaactc gccgaaacgt ccccatttga tcgccatcgg ccgtccgcac
     2881 agctcgcaga tctcgtccgt cggcttcatc aatgccgggg cattctcggc ttccgccaac
     2941 gccagcttta acggctgaaa aaactccagt accacggggc tccatgcacg gttgcctgcg
     3001 gcgatctcgt cgagctcttc ttccatatgg gcggtgaagc gcagatcgac cacgcccggg
     3061 aagtagctgg tcagcagctc gttgacgtcg cgaccaagct gggtgggctt taacgcccgc
     3121 ccgagttttt cgacgtacgt gcggtccatg aggttcgaca ggatcgtggc gtaggtgctc
     3181 ggacggccga tcccgttctc ttcgagcgct ttgaccaacg tcgcctcggt aaagcgggcg
     3241 ggcggctcgg taaaatgctg ctcggggaag aggtcgatca agttgagcgc ctcgccgggc
     3301 ttgaggtcgg gcagtgcccg agtgtcggcg tcctcgtcgt ccccttcctc cgtccgctca
     3361 aagtacacgg cgcggaagcc agggaagcga aggtggctcg cggtcgcgcg caacaggaat
     3421 gtgtcgccgc cgcgcggctt cgcgtggatg tcaaccgacg tcatgtcgat taaggcgtcc
     3481 gccatctgtg atgccacggc acgctgccag atcaactggt agagccggta ctggtcgttc
     3541 gtcagaaacc gcttgatgcg gtccggctcg cgcagtaccc ccgttggacg cacggcttcg
     3601 tgggcttcct gggcgccctt cgccttcttt gtgtatgtac gcgcctgctc tggcatgaat
     3661 tgtttgccga acctgtcggc gatatacgag cgaatttccg ccttcgcaac gtcagacatg
     3721 acggtggagt cggtgcgcat gtaggtaatc agtccgactt cgccctccga cccgatgctg
     3781 cggccctcgt agagctgctg cgctatggtc atcgtccgct tcgccgtgaa gccgagccgg
     3841 cgcgaggcct cctgctgaag cgtgctggtg gtaaacggcg ccgccggccg ccgagattga
     3901 ggacgttgcc gtacctcggc gacttcatac gaggacgtgc gcagctgttc gatgatcgca
     3961 ctggtctgtt gctcgttgtt gagctcgagt tcccgcttct tgccggcgta gccctgtaaa
     4021 cgggcgcgga acgattccgg tgccccggct gcttgggcca attcggcctc gatggaccag
     4081 tactcccgcg ccgtgaactc ttcgatctca tgctcactgt ccacgatcat gcgaagcgct
     4141 acggactgaa cgcggccggc agacgtcccg cggcgcactt tgttccagag cagcgggctg
     4201 atcttgtagc ccaccaggcg gtcgagcaca cgccgcgcct gttgagcatt cacaagcggc
     4261 atgtcgatgt cacgtggatg cttgaacgcg tcggtgatcg catcgcgtgt gatctcgtgg
     4321 aagacaaccc gtttgtgggg cgtattttcg aggtcggcag cctgcaccag atgccacgaa
     4381 atcgcttcgc cttcgcggtc cgggtccgtt gcgaggaaga ccgattgcgc gtttttcgcg
     4441 gcccttttta tctggtttac gacgtccgct ttgtctttgg ggatgatgta atgcggcgcg
     4501 aagtcgcgtt tttcatctac gccgagccgc gacttcggca gatctcggac gtgcccgacc
     4561 gatgccctaa cgtcgtaacc ggcaccaaga atgctggcca ccgtcttcgc cttggccggc
     4621 gactcgacga tcaccaggtt cttttttgct tgttttgcct gtggcattca acttccgttc
     4681 aatcaccgtg ccatgcacgg agcgttaact gactaccaat aatttacgca gtatactcgc
     4741 ccgcggtttc acgaagccgg acgtactgca tgccgccgat ctgccgaact acacccttta
     4801 gctccaaaag cgccaggccg ccgcttactt cggcggctgc gaggccactc cgtcgtgtga
     4861 cgtcgtcgat gtgaatcggt tcagccgtaa ggcagctgag cagcgtggat tcaacggccg
     4921 tcgcgggcac ggcagccgct agttccgcct gctgcgccgc cgcagcgagg ttgagctcgg
     4981 agagcacgtc ttccgcagtc cccgtcaact tcgcgccttc ctgaatgagg gcgttcgttc
     5041 ctttgctcat cggagaaaag atgctgccgg gtactgcaaa tacctcacga ttgtcatcgg
     5101 cagcgagctt ggccgtcaac agggctccgc tgcgttccgg cgcctctgtg atgacgacgc
     5161 cgagcgtcag acccgcgatg atacggttcc gtcgtgggaa taggtccttc tgaggccgcg
     5221 ttccgagcgg gagctcgctg accagagccc catgctccac gatctgccgt gccaggcgct
     5281 cgtgtgctgc cggatagatg atatcgacac cgcaagccat gacagcgatg gtgcgcccgc
     5341 ccgcgcccaa cgcggcctcg tgcgcggtcc tatctacgcc tctggcaagt ccgctaacga
     5401 tggtgatgcc ggatcgcgcc aagtctcctg cgatatgctc cgtgacctgg cggccatacg
     5461 ccgtcatgcg gcgcgttccg accacggcga gtgcaagttc atcttcagta gtgattgtac
     5521 cacgtacgta cagcactggc ggccggtcat acgactcctt gagtagtggc gggtagagcg
     5581 ggtcgttcca cgtgagagcg gtgacaccga ttcgctgcag ggcggctagc tcgcgctcgg
     5641 gcgagatgcg tggacgcgct gcgctaattg cccgcgcact tcgatcgtca attccggccg
     5701 ccctgagatc tgcgtcgctt gccagccacg ctcgctcgag ggtgccgaac gcgcgctcca
     5761 gccgctcgaa tcgggcccgc ccgatcgtcg gtatgcggtt gaaagcgagc cagtacgcag
     5821 tatcttccag ttggattccc cctggaccgt tcgtgcttgg cggattgtag cagcgacgcc
     5881 taaaatcaac cacggtactt gacgaggcgg caccgcttcg cccgcgcccg cagcgagaaa
     5941 aggcttttac ctggatggaa cgtaatccgt ggttccgtgc cctcctgatt ctgggcgtag
     6001 cattcctcgg cgtgcaactc tttctgatcg tctggcactt cggatcgcac ttcgcccaga
     6061 cgatcctgat cttcttcctc gcatggatgc tctccttcat tctcaatcca gccgtagacg
     6121 tcgtcgccag gcgctggcgg ctcggccggc cggcggccgt atcaatcgtg tttctcggca
     6181 taattgccgt cgtcgcgctc gtggggtttt tggtggtgcc gccggcggtc aagcaggtcg
     6241 ccgccctggg cgacaaggtg ccggagtacc ggcagaatac gggcaaactg gtcacagact
     6301 tgcagtcatg gctcgatgag cgccacatca atatcgatat caccggcgtc aacaccgagg
     6361 atctgagcaa gcagatcgat aagaccggtg ccagcctcgc tcaaaacgcc cttggactcg
     6421 caccgaaagt gatcgaaggg atcttcgaca ccgtgatcat cctcgtcgtc tcgttctata
     6481 tgatgctcga cgctccccgt atcagtgcgg ccatgatcag cgtcactccc gagcgatacc
     6541 gtcgcgacgt gcgcatgttg tttgccagca tcgaccacag tttcggcggc tatatacgcg
     6601 cgtcggtggc gctggcgctg ctctacgccg cgggcacggc gttgacgatg tgggggaccg
     6661 gaatcccgtt cgtcctgccc gtgagcatgt tcgcaggctt catgctcatc attccattta
     6721 tcggcgacgt tatcgcagtc ataccgacaa tcgtgatcgg gttgttcacg acgtcgctcg
     6781 tgcatgtcat cattgcgctc gtggcgatga tcgcgcttca acaactggtc ctacaggtca
     6841 ttcgacctaa gctgatgggc aaaagtgtgg gcctgcaccc attgtgggtg ttggcggcat
     6901 tcttcctcgg tgcccgcgcc gcggggctgt ggggggcgct gttcagcgtg ccgattgcgg
     6961 cgatcatcca gtctgtggtc cagttgtact actaccgagt gaccgggcgt ccgcaaccgg
     7021 cggcactcgc ggccctgatg cgcgaagcaa cagcgccgtt tccgttgcga ccggagccga
     7081 atctgccgcc cgaagacgaa tcacgagacc gcccggcggc ggatggtacg ccgattccgg
     7141 cagccacctc cggcgggccc ggccggcggg gcgacgatcg cgactaagtc ggatttggtc
     7201 cgcgaatcca gccatttctt gaagaggacg attgatgcgt gtactcgtgg tcggcttccc
     7261 gctgccgaac ccgcagtttg acaactacac cctgctcaat tcgccgtcat ggtttgatta
     7321 cgacgcggtc atcgtggagc cggagtcggt atcgacggtg atagagaatg tagtcaatcg
     7381 aaaggaagag cacgaaaccc gggctgaaga gccgatctta aaccggccga caacgccatt
     7441 cgcggttgcg cttgccgaca tgatcaggcg acgccgggcc gaggcgtcac aactgctggc
     7501 gaacggcggt gtgatcgccg tgtttacccg tcccaacatc gtccaccagg aagtcgttgg
     7561 ctttccgggc tgcgaccggt acgcgtggct gccggcgccg gcaggtgtga gctgggattc
     7621 gccgttcctc gttcgggcag aaggaacaga agtacacgcc tccgattcgc ggcatccgtt
     7681 tgcgaggatg gtggaccggc atcgcccgtg gttcttctac cgcgcccgca tcgacgaaaa
     7741 tgcgccgggc ttccccacgt atggcagcgt atttgcgcgg agctacggtg gtgtcgccat
     7801 cggcgccgac ctgaaagtcg gtgcgggccg cgtgatcctg ttgcccgcct tcgctccgct
     7861 tgcctacggt gaccagcggt tcgagctcgc gagcgagttc ctggaggcgc tgcgtctttg
     7921 cacgggacag caggccgata cgaagccgcc cgcgtgggtt tcggagctgt cggtgcctgg
     7981 ttctgaggaa ctggaagcgt ccgagcgcga agcaacggaa gcgcttgcgg ccgcggaaac
     8041 ccgggtcgcg gaggctcagg cgagtctcac gcagttgacg aaataccggg ccctcatctg
     8101 gcaggaaggc cgcttcggac tcgagcccgc ggtgtgcgac gcgtttcgcg ccattgggtt
     8161 tgaagttgaa tcagacccgg acaagcccgg gtggatcgcg gatggacgcg tccgcgcgtt
     8221 ctacgaagtc gaagcctcta ccgaagcggt gctcgagtac ccctactttc gcctgcaaaa
     8281 gcgccttgaa aaggacctga tcgatacaaa agagcccaag cacggcctga tcgtcgtcaa
     8341 cgggcaacga ctcagcccgc cggtgaaccg gaacgcccag gtaagcgaaa cgctgcgtat
     8401 cgccgtagac aattaccggt acggtctgct gacaaccgag cggctgttcg agattgtaaa
     8461 agcggtgctc gcggaccctc tcaacgcgcc cctgcggtca atgatccgat cgcgactgct
     8521 caacgccgtc ggggaagttg gcgccgatgt gattccgacc gccgaggaac tcgcctgact
     8581 agccggttac atgcacctca cggacgaggt cgaaccactc ggtcacgggc ggccctttca
     8641 agagccggaa gctcgagtgg atcaattctt gcaccttgga cgtgttgtaa tactcgaccc
     8701 aggatgcgcg cgagtcccac agcgaataga ttgtgtactt gcctggatgc tcgaccgatc
     8761 gcagtagcct tgccgttcga aaaccgggcg tctgtctggc gacgtcgtgc atctggtccc
     8821 agatagactc ccactcgtct agacgctccc gatcgatgtg ccagttcgca acctggtgtt
     8881 gcaagctcgt caccctcccg ccctgctgtt agtcgtcaag gctgagcacg gccagaaacg
     8941 cctcttgtgg gatttcgaca ttcccgacgc gcttcatgcg ttttttgccc tcggactgct
     9001 tttcaagcaa ttttcgcttg cgcgtgatgt caccgccata acactttgcg agcacatttt
     9061 ttcgcatcgc tcgaaccgtc tcgcgggcga ccacccgacc gccgatcgcg gcctgtatgg
     9121 gcacgtcgaa catctggcgt gggatgaggg agcgcagctt ttcgaccagc cggcgaccct
     9181 gttggggtgc acggtcggcg tgggcaatga gcgataaagc atcgacgggc tggccgttca
     9241 caagcacgtc gagcttaacc agcttcgccg cctcatagtg ggaaaagttg taatccaggg
     9301 aggcgtagcc ctgcgttcca gacttcaatt ggtcgtaaaa atctaccagg atttccgaaa
     9361 gcggaatctt gtactccagc agtacccgag cctcgccggc ggcgctgccc gcgccatcgc
     9421 catgctccag gtactccatc ttgttgaatg cgccccgccg cgtcgtgacc agctccatca
     9481 ccgcgccgat gtagcggctg ggtgtgatga cggagatatt cagccagggt tcgcggacct
     9541 cctggacctt ttccggcggt ggaagctcag ccgggttatc gacgttgagc tctttaccgt
     9601 cgctcagggt cacctggtac tcgacgctcg gggcagttgc aatcagctcg agaccgtatt
     9661 ctcgttccaa tcgctcctga acgatctcca tgtgcagcac gcccaggaag ccgcagcgaa
     9721 aaccgaagcc aagcgccatg ctcgactccg gctcgtacac cagcgaggcg tcgttgagct
     9781 gaagcttgtc gagcgcgtcg cgaagcagcg gataatcgtt ggcttgctgc ggataaatgc
     9841 cggcgaagac catcggtttg gccggctggt atcctttcaa cggttcatgt gcaggccgcg
     9901 ccgcgtctgt aaacgtatcg ccgacctggc actccttgac gttcttcagg ccggtggcga
     9961 catagcccac ctcgcccgtt accaactccg ttcccggaag cagtccgggg cggaacgtac
    10021 cgacctcgat cggctgcact gtcaggccgc tcgacatgag cttgagcggc ctgcgcccgt
    10081 ttaattcgcc atctacaaca cgcacatatg cgacgacgcc tttgtaggcg tcgtacttgg
    10141 agtcgaaaat tagcgcccgc gccggcgcct cggcccgacc ccttggtgga tgcacgcgct
    10201 tcacaactgc ttccaagatg tccgtgatgc cagtcccttc ttttgccgat gcgaggatca
    10261 tttcgtcgcg gtcgaagccg atgacgtctg ccagttcctg catcactcgc tggggctcgg
    10321 cgttgggcag gtcgatttta ttgatcacgg ggatgatctg aaggttgtgc tccagcgcca
    10381 gatacacgtt ggcaagcgtc tgcgcttgaa tcccctgcgc ggcgtccacc accaagatgg
    10441 cgccctccgc cgcggcgaga gaccgtgaca cctcatacgt gaagtcgacg tggcctggtg
    10501 tgtcgatcag gttgagctcg tactcgatcc cgtcccgcgc ggtgtagaac atgcggacgg
    10561 caacggcctt gatcgtgatg cccttttcgc gttcgaggtc catcgaatca agcacctgct
    10621 cctccatctc gcgtgcagat agagtgccgg taacttcgag ca
//
LOCUS       DATCPE010000063         8658 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U7219
            k141_173149, whole genome shotgun sequence.
ACCESSION   DATCPE010000063 DATCPE010000000
VERSION     DATCPE010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955033
            Sequence Read Archive: SRR1586279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 8658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 16:49:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 640
            CDSs (total)                      :: 631
            Genes (coding)                    :: 627
            CDSs (with protein)               :: 627
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8658
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173149"
                     /isolate="SMAG_U7219"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..832
                     /locus_tag="VIM45_01945"
     CDS             <1..832
                     /locus_tag="VIM45_01945"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY8820081.1"
                     /translation="PLRGVPVGWRVRYRWTLNALRLLAAILVVLALSRPQTGKASARI
                     PAQGVDIVIALDVSGSMLDPGLSASSKLTGAKLAIREFIAQRKNDRVGFVTFESESRV
                     MSPLTLDYRALQQIVDRSDNGLLPDGTAIGLGIADAVNLLRESRAKSRVIILATDGEN
                     NQHRLEPEQAADIAKSLKMKLYTIGMFAKGESAATGQIDEKLLRQIAEPTGGFYARAQ
                     SGDQLHKIFDTISKLETSQIERQHYTSYFELEGWLLAPAMGLLVLEVVLSGTVFRRTP
                     "
     gene            849..1973
                     /locus_tag="VIM45_01950"
     CDS             849..1973
                     /locus_tag="VIM45_01950"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY8820082.1"
                     /translation="MPRIAFGAPELLPMVLLAVLAAGLFVWVFSRKRSARARYRGTSM
                     AALVSAATSPGHQVFKAALVVLVMLLLALAAARPQIGTHKTLLQREGTDVIVALDVSL
                     SMSAQDAKPSRLERAKGAIGALFDHLQGDRVGLVTFAGSAELRFPLTTDVDAARKVVQ
                     GLTFKDGGLRAGTSIGEALRQATEGFANDNTRSKILVLVSDGEDLGDDATGAATFVKS
                     QGIALDTIGIGQDSPVPVLVLNPRTGLLESRTDPTTSQALLTSADPKALQQLASSNQG
                     HFFDGNSDDYAVQLADEIGRLQKTRFESGEGDVPLERYQILAGIALAMLMLDYMLPAG
                     RRRKVDSGGGWLSPVLRRIRGGDRPAAAAAPVDSHIGVNQ"
     gene            1970..2989
                     /locus_tag="VIM45_01955"
     CDS             1970..2989
                     /locus_tag="VIM45_01955"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY8820083.1"
                     /translation="MTRSDRQTTLYRPTISRRHNRRSSMFLAGAIVLAACSVACSRPS
                     AQRLNDEGNKAFNRGDYSRALENYRKAQVERPDQAAFNYNAGNALNQQGEFPRAIQES
                     LRATQAGISDVRVRAYYSIGADYYREGKFKEALDAYKKALLLDPNDVDTKYNVEVIQR
                     RLDKEAEQQKQLEEQAKQDQPEQQDQQSEQGQQPQQNQQNSQGQQPGQQQGQQPQQGQ
                     GQQQGQQNPNGQQGQSGQQGAGQQAGQPNGQQQGGQPSQPTGSGTGGQAASSAAPGAN
                     GTPGPSVREQQAGLNQDLKNAIDSYGKQPTIEEALRILDIIAEQERLAQADQGNRSDP
                     RVRDK"
     gene            3143..4582
                     /locus_tag="VIM45_01960"
     CDS             3143..4582
                     /locus_tag="VIM45_01960"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEY8820084.1"
                     /translation="MTDRFTSEHKRRFDAAVHWIESFIRPITGTAPQKTRADWYTDGP
                     GRLDRMATLLTSFGHPERRYETLHVTGTSGKGSVCTYLGSVLRASGRRTGVHATPYLQ
                     TPVEKLQVDGRYASGEELAGLVDEFRSLLPEGRAATDGLPYPALWVALTYLYFARKHV
                     DSAVIEVSTGGRFDWTNTLNPRVTVVTTVGPDHLMSLGPSLADVAYHKTGIIKPGIPS
                     VTGVHLPERLIIEQEADRQCSPLLRLGVEFDYSVRRCTESGTIFDFESHVSGLRSCKS
                     LEIGMLGRYQAFNAALATAALGSYGTENSAIDEDALRTGLRDARLPGRMELIQRGPDV
                     LLDGAHNPEKAAALADSIAAIFPDRRLLLVIGALSTKDVSGILEPFAGMTRTATLTIP
                     HVLGKSGLDPERMARTAAVLGINAHVEPDPAVAVQTAMASAGPDDLVLVTGSLYLVGE
                     VRRLWVPDEQVLATGYSTPGGATSAAKHA"
     gene            complement(4557..6854)
                     /locus_tag="VIM45_01965"
     CDS             complement(4557..6854)
                     /locus_tag="VIM45_01965"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HEY8820085.1"
                     /translation="MSQANWIEWFREQPAGPDRPKSFYAQMPSWLLSWESWLTFALAT
                     ITFLSVSRSIDSAHWVEDMPSLALVSFLALLTGFGLSRIKINEVFLHLLALLTGAPIV
                     VGLTLNYMGETSLRLGLAHLWHRWGDWIDVIRSGGITSDNMPFVTLVLALAWIAAFLS
                     SWAIFRWHNAWLALIPGGMGLLTNISYLPGQFSTDFVVFLFGSMLLVMRVNMLNRISD
                     WQHRGIAFPRFLSLTLLNATIWIAAGLLIIAWWIPLAGESRAIASAWEGVTQPFSNAS
                     ADWGRLFSSIDAKREVPLHTFGPTLPLQGKVVLSDRVVAEVDFQDQSNAGHNLVATHY
                     DEYTPAGWKRGGRETADLGPNGVKIGDQQPINQDVYKDRKDIPADVIVDSPNGVLLSV
                     GQPLQTSVDARAELVNGGNRPDITAIRPKKDLKRGDQYTTIGSVSVAPEDSLRKATPD
                     YPEYIKDRYLQLPAELPQRVRDLAVQVTANAPTAYDKARTLEDLLRTYQNTYDVPIVP
                     PGRDAVDFFLFDTKRGYSDYQASAMVVLLRALGIPARLATGYAVEEFDLNTHRYIIRE
                     KHADSWPEVYFTGYGWVEFSPFGGAPLVSRPFSGDDTGPAIQDDPAASGAPGIFDGEF
                     GPEIPLDQGTAGLLKVHHPFNWLPVYIALTFLAIIVISGGFVRLAWEYGLRGLDRPAR
                     TWEKTLRLASWTRLRPKPNQTPNEFAGEVARRTQLTEEPRIVADGYLRVRYGRKGLDR
                     QEQEKVDSAWVRIRNRLIKRALRLK"
     gene            complement(6883..8136)
                     /locus_tag="VIM45_01970"
     CDS             complement(6883..8136)
                     /locus_tag="VIM45_01970"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEY8820086.1"
                     /translation="MHGVRQFHLQRSVLIICLLMALCVGFALATGFWLLFRLAYVLLA
                     AVPLCFVWSRINLSGLEVTVERRIDRAQVGQTTDERITVRNRSLFPKVWLEVDDPTTL
                     PGHRPGRVVTLGGRQRRSWRTQTLLTRRGVYDVGPVAISSGDPFGLFRMTRRLGPQSS
                     IIVYPPFFDLPHFNVPPANLPGEGRFRKRTHYVTPNASGVREYVPGDSFNRIHWRTTA
                     RMGKLMVKVFELDPASDIWIALDLQQSVQAGSEDESTEEYAVKVAASVARHFLNQNRN
                     VGMISFGERLEIVETERGGQQLTHILESLAVARANGDVPIGDLLNVEGKRFGRHTTLV
                     VVTPSVDEAWVLSLLQLTQRGVKVAVVLLEASTFGGQSNALMVVSALAAADIWTYLVK
                     RGDNLSASLSPAGEESAEIASSAEH"
     gene            complement(8167..>8658)
                     /locus_tag="VIM45_01975"
     CDS             complement(8167..>8658)
                     /locus_tag="VIM45_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEY8820087.1"
                     /translation="PEAQLDRFMLRVHLGYPSKTDEIIIMDRQQMTHPIDDLEQITDA
                     SEVLRLQTGVKAIYVDPLIKQYIVEIVAATRNHEAAYLGASPRGSLALYRSSQALALM
                     DGRDFVIPDDVKALAYPTLGHRVIASPSARVKNVTTASIVNDCLSRIPVPGARARGAY
                     SAG"
BASE COUNT         1729 a         2630 c         2719 g         1580 t
ORIGIN      
        1 accgctacgt ggtgtgcccg ttgggtggcg ggtgcggtat cgctggacct tgaacgcgct
       61 ccgattgctg gcggcaatac ttgtcgtact cgcgctgtcg cgtcctcaaa ccggtaaagc
      121 cagcgctcga atcccagctc aaggcgtcga cattgttatt gccctcgacg tctccggcag
      181 catgctcgat cccggactta gcgccagcag caaactgacc ggcgccaaac tggcgatcag
      241 agagttcatt gcgcagcgga aaaacgatcg agttggattc gtcacgtttg aaagcgaaag
      301 ccgcgtgatg agtccgctca cgctcgacta tcgcgcactg cagcaaattg tcgatcggtc
      361 tgacaacggc ttgctgcccg acggcaccgc aatcgggctg ggcattgccg acgccgtgaa
      421 cctgctgcga gagtcgcgcg ccaaaagtcg tgtgattatc ctggcgaccg atggagaaaa
      481 caatcagcac cgattggaac ccgaacaagc ggccgacatc gccaagtccc tcaaaatgaa
      541 gctctatacg attggcatgt tcgccaaggg cgaatcggcg gcgacgggcc agatcgacga
      601 aaagttattg cggcagatcg ccgaacctac cggcggcttc tatgcgagag cgcagtccgg
      661 cgatcagttg cacaagatat tcgacacgat cagcaaactc gaaacgtctc agatcgaacg
      721 ccagcactat accagctact tcgagctcga aggttggttg ctggcccccg caatggggct
      781 tctcgtcctg gaagtagtgt tgtcaggtac cgtttttcga cggacgccgt aacggaggaa
      841 aggtcacgtt gccaaggatc gcttttggag ccccagagtt gctgccgatg gtgctgctcg
      901 ccgtgttggc ggcagggctc ttcgtctggg tcttctcgcg caagcgatcc gcccgggcgc
      961 ggtatcgtgg cacgtcgatg gcggcgctgg tgagtgctgc cacgagcccg ggtcaccagg
     1021 tgttcaaggc tgctctagtc gtcttggtga tgctgctgct cgcgctcgcc gccgcccgtc
     1081 ctcaaatcgg cacccacaag acgttgcttc agcgcgaagg caccgacgtc atcgtggcgc
     1141 tggacgtttc gctcagcatg agcgcgcagg acgctaagcc ctcgcggctg gaacgggcaa
     1201 agggcgctat cggcgcgttg ttcgaccatc tgcagggtga tcgggttggc ctcgtcacgt
     1261 ttgcgggcag tgccgagctt cgatttccgc ttacaacaga tgtcgacgcg gcgcgaaaag
     1321 tggttcaggg cctgaccttt aaggatggcg ggctgcgcgc cggcacgagc attggcgaag
     1381 cgctgcgaca ggcaactgaa ggatttgcca acgataacac ccgcagcaaa attctcgtgc
     1441 tggtgagtga tggcgaagac ctcggcgacg acgcgaccgg cgcagcgacg tttgtgaaaa
     1501 gccagggtat cgcattggac acgatcggta tcgggcagga ctccccggtg ccggtgctgg
     1561 tgctgaaccc gcgcaccggt ctgctcgagt cgcgtaccga ccctactact agccaggcgc
     1621 ttttgacgtc cgccgaccct aaggcgctgc aacagctcgc gagcagcaac caaggacact
     1681 tctttgacgg caacagcgat gactacgcgg tccagctcgc cgacgaaatt ggccgcttgc
     1741 agaagaccag gttcgaaagc ggagagggcg atgttccgct cgagcgctat cagatcctgg
     1801 ccggaatcgc ccttgcaatg ttgatgctcg actatatgct tccggcaggg cgccgtcgca
     1861 aggtcgattc gggcggtgga tggttgtccc cagttttgcg ccgaattcgc ggaggggatc
     1921 gccctgcggc tgcggccgcg ccggtcgatt cgcacatcgg ggtcaaccaa tgacgagatc
     1981 cgaccgtcaa accacgctct accgtccgac gatctcgcgg cggcacaatc gacgatcgtc
     2041 gatgttcctg gctggggcga tcgtactggc ggcatgcagc gtcgcttgtt cacgcccgtc
     2101 ggctcagcgg ctgaacgacg aaggcaacaa ggcgttcaat cgcggcgact acagcagggc
     2161 cctggagaac tatcggaagg cgcaggtgga gcgcccagat caggcggcgt tcaactacaa
     2221 cgcgggcaat gcgttgaacc agcagggcga gtttccgcga gccatacaag agtcgcttcg
     2281 tgcgacgcaa gccggtatct cagacgttcg cgtccgtgcc tactacagca tcggcgcaga
     2341 ctattaccgg gaagggaagt tcaaagaggc gctggatgcg tacaagaagg ccttgctgct
     2401 tgatcccaac gacgtggata ccaaatacaa cgtcgaagta attcaacgcc ggctcgataa
     2461 agaggcagag cagcagaagc agctcgaaga gcaggcgaaa caggaccagc cggagcaaca
     2521 agaccagcaa agcgagcaag ggcagcagcc gcaacagaat cagcagaatt cacaaggcca
     2581 gcagcccggc caacagcagg gacagcagcc gcagcaaggt caggggcagc agcagggcca
     2641 gcagaacccg aacgggcaac aagggcagtc cggtcagcaa ggtgccggac agcaggccgg
     2701 ccaaccgaac ggccagcaac agggtggaca accgagccag ccgaccggca gtggaacggg
     2761 cggtcaggct gcatcctcgg cagcgccggg ggcgaatggt acgcccggcc cttcggtccg
     2821 agagcagcag gctgggctaa accaggacct caagaacgcc attgactcct acggcaaaca
     2881 accgacgatt gaagaggcgc tgcgcattct ggacatcatc gccgagcaag agcgcctcgc
     2941 gcaggcggat cagggcaacc gcagcgaccc gcgggtgcgg gataaatagc gcgggtctcc
     3001 gcagcccgca agatttggaa taagacctta cccttccggt tcctctcgtt tgcccgccgc
     3061 cccgcaaagg tcggatgctg taggtgcggc cggcggtgca tgcagcccac cgcgaagact
     3121 gtatcggcgg caggttcgac cgatgacgga caggttcact tcggaacaca aacgtcgctt
     3181 cgacgcggcc gttcattgga tcgagtcctt catccgtccg atcaccggta ccgcgccgca
     3241 aaagacgcgc gcggactggt acacggacgg ccccggccgg ctggaccgaa tggcgacgct
     3301 gctcacctcc ttcgggcacc ccgagcgccg ttacgagacc ctccacgtga ccgggacgtc
     3361 ggggaagggc tcggtctgta cgtatctcgg ttccgtcttg cgggcctccg ggcgtcgcac
     3421 gggcgtgcat gcaaccccgt atttgcaaac gccggtggaa aaactccagg tagacgggcg
     3481 ctatgcgtcg ggcgaagagc ttgctgggct tgtcgatgag tttcgctcgc tgctgcccga
     3541 aggccgggcg gcgacggacg gcctgccgta tccggccttg tgggtcgctc tcacctatct
     3601 ctactttgcc cgcaagcacg tggacagcgc ggtgatcgaa gtcagcaccg gtggccggtt
     3661 tgactggacg aacacgctaa acccgcgagt tacggtggtt accaccgttg gacccgacca
     3721 cctgatgtcg cttggaccgt cgctcgcgga cgtggcttat cacaagaccg gcatcatcaa
     3781 gcctggtatt cccagtgtga ccggagtgca tctcccggag agactgatca tcgaacagga
     3841 agccgaccgg cagtgcagcc cactgttgcg gctcggtgtc gagttcgact actcggtacg
     3901 gcggtgtact gagagcggaa cgatcttcga ctttgaatcg catgttagcg gccttcgctc
     3961 ttgcaaaagt ctggaaatcg gaatgctcgg ccgctaccag gccttcaatg ccgcgctggc
     4021 gactgcggca ctgggttctt atgggacaga aaacagcgct atagacgagg atgcgttacg
     4081 caccggtttg cgcgacgccc ggcttcccgg ccggatggaa ctgatacaac gcggtccaga
     4141 cgtacttttg gatggagccc ataacccgga gaaggcggcc gccctggccg attcgatcgc
     4201 cgcaattttc cccgatcgcc ggctgctgct cgtgatcggg gcgttatcaa ctaaagacgt
     4261 gagcggaata ttggagccat ttgcgggcat gacgcgcacg gcgaccctga ccataccgca
     4321 cgtgctcggc aagtccggtc tcgaccccga gcgcatggcc aggaccgccg ccgttctagg
     4381 gatcaacgcc catgtggaac cggatccggc cgtcgctgtg caaaccgcca tggccagcgc
     4441 cggccctgat gacctggtgc tcgtcacggg ctcgctatac ctggtcggag aagtcagacg
     4501 cctatgggtc cccgacgagc aggtccttgc gaccggatat agcacgccgg ggggcgctac
     4561 ttcagccgca aagcacgctt gatcaggcgg ttacggatgc ggacccaggc gctgtccacc
     4621 ttttcctgtt cctggcgatc caggcctttg cgaccgtacc gcacccgcag ataaccgtcc
     4681 gccacgatgc gcggttcttc ggtgagctgg gttcggcgtg cgacctcgcc tgcaaactcg
     4741 ttcggcgttt gattgggctt cggccgtaat cgtgtccaac tggccagtcg caacgttttt
     4801 tcccaggtcc gtgccggtcg atccaggccg cgcagcccgt attcccacgc caggcgcaca
     4861 aagccgcccg aaattacgat gatagccagg aacgtcagtg cgatgtaaac cggcagccag
     4921 ttgaacgggt ggtgcacttt tagcagcccc gcggttcctt gatcgagcgg aatctccggc
     4981 ccgaactcgc cgtcgaagat gcccggcgca ccgcttgcag ccgggtcatc ctgtatcgcg
     5041 gggccggtat cgtcgccgct gaacggccgc gagaccagcg gagcgccgcc gaaggggctg
     5101 aattcaaccc agccgtaacc ggtgaaatac acctcgggcc acgaatccgc gtgtttctcg
     5161 cggatgatgt accggtgggt gttgaggtcg aactcttcca ctgcgtaccc agtagccagg
     5221 cgagcaggaa ttccgagcgc gcggagcagg accaccatcg ccgacgcctg gtagtcggag
     5281 tagccccgct tggtgtcgaa caggaaaaaa tccactgcat cgcgtcccgg cggaacgatt
     5341 ggcacgtcgt atgtgttctg ataggtgcgg aggaggtcct cgagcgtccg cgctttgtcg
     5401 tatgcggttg gggcgtttgc agtcacctgt accgcaaggt cgcgaacgcg ctgcggtagc
     5461 tcggccggga gctgtaagta ccggtccttg atgtattccg gataatcagg agtggctttg
     5521 cgaaggctgt cttccggcgc gaccgagacg ctgccgatgg ttgtgtactg atcgccgcgc
     5581 ttcaggtcct tctttgggcg gatagcagtg atgtctggtc ggttgccccc gttgacgagt
     5641 tcggctctgg catcgaccga ggtctgcagc ggttgtccca cgctgagcaa aacaccgttg
     5701 ggggagtcga cgatgacatc ggccggaatg tccttgcgat ccttgtaaac gtcctggttg
     5761 atcggttgct ggtcgccgat ctttacgccg ttcggtccga gatcggccgt ctcacgtccg
     5821 ccgcgcttcc aacccgccgg tgtgtactcg tcataatgcg tggccacgag gttgtggccc
     5881 gcgttcgatt ggtcctggaa atccacttct gcaacgacgc ggtcgctcag cactactttg
     5941 ccttgcaagg gcaacgtagg cccaaacgtg tgcaacggca cctcgcgttt ggcgtcgata
     6001 ctgctgaaca ggcggcccca gtccgcactg gcgttgctga aaggctgcgt tacaccttcc
     6061 catgcagacg ctatcgccct ggactcgcct gccagcggta tccaccacgc gatgatcagc
     6121 aggccagccg cgatccagat cgtggcgttc agaagggtca ggctgaggaa tcgcggaaat
     6181 gcgattccac ggtgctgcca atcgctgatg cgattcagca tattcacacg catcacgagc
     6241 agcatcgaac cgaataggaa cacgacaaag tcggtgctga actgcccggg caagtagctg
     6301 atgttcgtta gcaggcccat gccgccgggg ataagcgcca gccaggcgtt gtgccatctg
     6361 aagattgccc acgaagagag gaaggcggcg atccatgcga gcgcgagcac cagcgtgacg
     6421 aaaggcatgt tgtcactggt gataccgccc gaccgaatca cgtcgatcca gtcaccccag
     6481 cggtgccaca agtgggccaa accaaggcgt agcgacgtct cgcccatgta gttcagcgtc
     6541 agtccgacga cgatcggcgc cccagtgagg agcgccagca ggtgaagaaa tacctcattt
     6601 atcttgattc gtgaaaggcc gaacccggtg agcagggcga ggaagctgac cagcgccagc
     6661 gacggcatat cctcgaccca gtgggcggag tcgatcgacc gcgacacgct gaggaaggtg
     6721 atcgtggcca gggcgaacgt gagccaggat tcccagctga gcagccatga cggcatctgg
     6781 gcgtagaagg acttgggccg atcggggccg gcgggctgct cacggaacca ttcgatccag
     6841 tttgcctgac tcatctgccg cggtctccct cagtcctgcc gctcagtgct ccgccgacga
     6901 cgcgatttca gcgctctctt cgcccgcggg tgagagagaa gctgaaaggt tgtcgccgcg
     6961 cttcaccagg taagtccaga tgtcggcggc cgcgagggcg cttactacca tcaatgcatt
     7021 ggactggccg ccgaaggtgc tcgcttccaa cagaaccacg gccactttga cgccgcgctg
     7081 ggtgagttgt aacaggctga gcacccaggc ctcgtcgacc gagggcgtaa cgacgacgag
     7141 cgtcgtatgc cgtccaaatc gcttgccctc gacgttcagt aagtcgccga tcgggacatc
     7201 gccgtttgca cgcgcgaccg cgagcgattc cagaatatga gtgagctgtt gaccgccccg
     7261 ttcggtttcg acaatctcaa gccgttcgcc aaacgaaatc ataccgacgt tgcggttctg
     7321 gttgagaaag tggcgggcga ccgacgcggc gaccttgacc gcgtactcct cggtgctttc
     7381 gtcctcgctc cccgcctgca ccgactgctg cagatcgagc gcgatccaga tatcgcttgc
     7441 cggatccagt tcaaacactt tcaccatcag cttgcccatg cgcgccgtgg tgcgccagtg
     7501 aatgcggtta aagctgtcgc cgggcacata ttcgcgcacg cccgaagcgt tgggggtgac
     7561 atagtgtgtg cgctttcgga agcgcccttc tccgggcagg ttggcgggtg ggacattgaa
     7621 atggggaagg tcgaaaaagg gcgggtagac gatgatcgac gactgaggac ccaggcgccg
     7681 ggtcatgcgg aaaagcccga acggatcgcc gctgctaatc gcgaccggcc caacgtcgta
     7741 aactccgcgc cgcgtaagca acgtctgtgt gcgccaggat cgacgctgcc ggccaccgag
     7801 cgtaacgacg cggccaggac gatgccctgg aagcgtcgta gggtcgtcta cctccagcca
     7861 taccttcggg aacaggctgc gattgcgtac cgttatccgc tcatcggtgg tttggccgac
     7921 ctgtgcgcgg tcgattcttc gctcgacggt cacttcgagg ccagacaggt tgatgcgaga
     7981 ccagacaaag cacagaggca cggccgccag caacacatag gccagccgga acagcagcca
     8041 gaacccggtc gccagtgcga agcccacgca tagggccatc agcaggcaaa tgattaatac
     8101 cgatcgctgt agatggaact ggcggacgcc gtgcatgtat gcccctcgcc cgaagcgtga
     8161 ccgtggttat ccggctgagt atgcccctcg cgcgcgggcg ccgggcaccg gtattcgcga
     8221 caagcagtcg ttgacgatac tagcggtggt gacgttcttg acacgcgccg atgggctagc
     8281 aatgacgcgg tgcccgagcg ttggataggc cagcgccttt acgtcgtccg ggatgacgaa
     8341 gtcgcgcccg tccatcagcg caagcgcctg cgatgaccgg tacagggcaa gcgagccgcg
     8401 cggcgatgcg cccaggtagg cggcctcgtg gtttcgggtc gccgccacga tctcaacgat
     8461 atactgcttg atgagcgggt ccacgtaaat cgctttgacc ccggtttgaa gacgcaacac
     8521 ttcgctcgcg tcggtgatct gctcgagatc gtcgatcgga tgggtcatct gctgccgatc
     8581 catgatgatg atctcgtccg tcttgctcgg atacccaaga tgcacgcgca gcataaaccg
     8641 gtcgagctgc gcttccgg
//
LOCUS       DATCPE010000064         3818 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U7219
            k141_302803, whole genome shotgun sequence.
ACCESSION   DATCPE010000064 DATCPE010000000
VERSION     DATCPE010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955033
            Sequence Read Archive: SRR1586279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 16:49:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 640
            CDSs (total)                      :: 631
            Genes (coding)                    :: 627
            CDSs (with protein)               :: 627
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3818
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302803"
                     /isolate="SMAG_U7219"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..361
                     /locus_tag="VIM45_01980"
     CDS             <1..361
                     /locus_tag="VIM45_01980"
                     /inference="COORDINATES: protein motif:HMM:NF017736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase family
                     metallohydrolase"
                     /protein_id="HEY8820088.1"
                     /translation="KLELVIQEIARWHGDKAVANIEIDVHSGFRAPEHNRKIRRAARD
                     SQHQYGDAADVAIDANGDGKLTAIDSRMVGLAVEIVELKHPDLVGGLGLYTSGHSSTN
                     YVHIDVRGRRARWRGHG"
     gene            384..803
                     /locus_tag="VIM45_01985"
     CDS             384..803
                     /locus_tag="VIM45_01985"
                     /inference="COORDINATES: protein motif:HMM:NF021558.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2281 domain-containing protein"
                     /protein_id="HEY8820089.1"
                     /translation="MNDILRERLIRKLDTLPEEKVYQILDYIDFLESKYAPKLSTAPN
                     PLKRFAEGVEDTLRAGKMSANVIGGTMNVMNKAVGVLTDVANAGKSVATEIAGVASSM
                     TSPKAQPPQTGSASSTAPPASPSTQTPPTQPPGDQRK"
     gene            965..1315
                     /locus_tag="VIM45_01990"
     CDS             965..1315
                     /locus_tag="VIM45_01990"
                     /inference="COORDINATES: protein motif:HMM:NF018508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HEY8820090.1"
                     /translation="MKELPNFLRLIGGLLTDVRVATTDKLLVAGAIGYVFLPMDFVPD
                     FVPFIGEIDDVFLLVLALQRLIANADRSVVLQHWMGDPKQLRSLDLERVLVAAAFFLP
                     RRVRRRLRTIGRDI"
     gene            1369..3681
                     /locus_tag="VIM45_01995"
     CDS             1369..3681
                     /locus_tag="VIM45_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014265992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEY8820091.1"
                     /translation="MTSARAGLTADEARRRLEKSGPNAMPDTALHPLRRALSKLWAPV
                     PWMLEAAILLELVLGKYVEGAVIAGLLLFNAALGFFQEGRAQATLAALKSRLALNASV
                     RRDGVWKIVPAAELVPGDTVKLSLGAVVAADVRLVEGSVLLDQSMLTGESIPIEAGPG
                     AQTYAGALVRRGEAVAEVTATGARTKFGRTAELVRTAHVVSSQQKAVLRVVRNLALFN
                     GVLIALLVAYAYAISMPWAEIVPLVLTAVLASIPVALPATFTLAAAVGAQALAKLGVL
                     PTRLSAVDEAASIDVLCADKTGTLTNNELKVTTVHQMPGFDESQVMGMAALASSDGGQ
                     DPVDGAIRAVALQRAASNLPKLINFVPFDPATKMSEAMVTDSTGATLRVVKGAFAAVA
                     GLTQASPDASTTANELEAQGFRVLAVAVGAPAAMKLAGIIALSDPPRADSAALITELH
                     TLGVRTVMVTGDAPATAAIVAHAVGLNGAVSPPGNIPDRVRAEDFAVFAGVLPEGKYR
                     LVQAFQRGGHTVGMCGDGANDAPALRQAQMGIAVSTATDVAKSAAGMVLTQPGLVGIV
                     AAVREGRVTFQRILTYTLNTVIKKIVTVLFLAVGLVMTGHAILTPMLMVIIMVTGDFL
                     TMSLTTDKVQASPLPNEWRIGNLTIAGAVLGFCLLGFCTAVLAFGHFSMALGTAALNT
                     LALVALVFGSQTALYAVRERRRLWSLPGLWLNVSSAANLLIISTLAVAGILMTPLPIV
                     VVAGTLGAAVGFGFVLTVVKIPVFRRLRIA"
BASE COUNT          668 a         1168 c         1295 g          687 t
ORIGIN      
        1 caagctcgag ctggtgattc aggagatcgc gcgctggcac ggcgacaagg cggtggccaa
       61 catcgagatt gatgtgcatt cgggatttcg cgcgcccgag cacaaccgga agatccggcg
      121 cgccgcgcgt gacagccagc accaatacgg ggacgccgca gacgtagcca tagacgccaa
      181 cggcgacgga aaactcaccg cgatcgacag ccgtatggta ggtttggcgg tggagatcgt
      241 cgagctgaag cacccggatc tggtcggcgg gctcggcctc tacaccagtg gtcactcgag
      301 caccaactac gtacatatcg acgtgcgcgg ccggcgggcg cgctggcgcg gccacggctg
      361 acgactacgg gggagcaaag gggatgaacg acattctgcg cgagagattg attcgcaagc
      421 tggatacgct ccctgaggag aaggtctatc agattctcga ttacatcgac tttctggagt
      481 cgaagtacgc gccgaagctc tcgacggcgc cgaacccgct caagcggttt gccgagggtg
      541 tcgaagacac gcttcgcgcc gggaaaatgt cggcgaatgt gatcggcggg acgatgaatg
      601 tgatgaacaa ggcagttggg gtgctcacag acgtggctaa cgctggcaag tcagttgcaa
      661 cggagatcgc gggggtcgct tcgtcgatga cttcgccgaa ggcgcagccg ccccagacgg
      721 gttctgcttc atcgaccgcg ccgcctgcgt caccatcgac acagactccg ccaacacagc
      781 caccgggaga tcagcgtaag tgagagctca atcggcgcgc gcgagcgcgg gatcatcgcg
      841 aaagaaaagc gcgagtcgcg ctcgcccggc tggcgcggct cgttcgtccg cgcgcgcccg
      901 ggaggcggaa gcgccatcag cagcgggacg aaggccgagc ccgaaacgaa cggttatctc
      961 gacgatgaag gagctgccca attttctgcg gctcattggc gggctgctga ccgacgttcg
     1021 cgtggcgacg accgacaagc tgctagtcgc cggcgcgatc ggatatgttt tcctgccgat
     1081 ggatttcgtt ccggattttg tgccgttcat cggcgagatc gacgacgtat ttctgctcgt
     1141 gcttgctctc cagcggctga ttgcgaatgc cgaccggtcg gtggtgctcc agcactggat
     1201 gggcgacccg aaacaacttc gctcgctgga cctggagcgt gtgctggtgg cagccgcgtt
     1261 ctttttgccg cgtcgggtgc ggcggcggct gagaacgatt gggcgggaca tctagcgtcg
     1321 gaaaggcgca cgattgaagc tttcgcaagc tcggagcgtg gggaccgcat gacatccgca
     1381 cgcgcgggcc tcacggccga tgaggcccgc cgccggctgg agaaatccgg cccgaacgcc
     1441 atgcccgaca cggcactgca tccgctgcgc cgagcgctga gcaaactctg ggcgcccgtt
     1501 ccatggatgc tcgaagccgc gatcctgctg gagttggtgc tcggcaagta tgtggagggc
     1561 gcagtcatcg ccggtctcct cttgttcaac gccgcgcttg gtttttttca ggaagggcgc
     1621 gcgcaggcga ccctcgccgc gctcaagtcg cggctcgcgt tgaacgcgtc ggtccggcgc
     1681 gacggtgtct ggaaaatcgt gcccgccgcc gagctggtgc ctggtgacac ggtgaagctc
     1741 tccctcggtg cggtcgtcgc ggccgacgtg cgactggtcg aggggtcggt tctgctcgat
     1801 cagtccatgc tcaccggcga atcgatccct atcgaggctg gccccggcgc acagacatac
     1861 gcgggagcgc tggtgcggcg cggtgaagcc gttgccgagg tcacggcgac cggggcgcgc
     1921 accaagtttg gccgcaccgc ggaactggtc cgcaccgcgc acgtcgtgag ctcccagcag
     1981 aaagccgttt tgcgcgtggt ccgtaacctc gctctgttca acggcgttct tatcgcgctg
     2041 ctggttgcct atgcctatgc gatctcgatg ccgtgggccg agatcgttcc gcttgtgctc
     2101 acggcggtcc tggcttcgat cccggtcgcg ctacccgcga cgtttaccct tgcggcggcg
     2161 gtcggcgcac aggctctggc caaactcgga gtcctgccga cgcggctctc cgcggtggac
     2221 gaagcggcgt cgatcgatgt cctgtgcgcc gacaagacgg gcacgctgac gaacaacgag
     2281 ctgaaggtga cgaccgtcca ccagatgccc ggcttcgacg aatcacaggt catgggaatg
     2341 gcagcgctgg cgagctcgga tggcgggcag gatccggtcg acggcgccat tcgcgccgtc
     2401 gccttgcaac gcgccgcgtc caacttgccg aagctgatca acttcgtccc attcgatccc
     2461 gccacgaaga tgtcggaggc gatggtgacg gattccaccg gagccacgct gcgtgtggtg
     2521 aagggagcgt ttgcagccgt cgccgggctc acgcaggcat cgcccgatgc gtcgacgacg
     2581 gcgaatgaac ttgaggcaca gggcttccgc gtgctggccg tggcggtggg tgcgccggcg
     2641 gcgatgaagc tggcgggaat catcgccctc agcgacccgc ctcgcgcaga ctcggcggcg
     2701 ctgatcacgg agcttcacac gctgggtgtg cgcacggtga tggtgacggg cgatgcgccg
     2761 gcgacggcag caatagttgc gcacgcggta gggctgaatg gcgccgtctc gccacccggg
     2821 aacatcccgg accgggtaag ggccgaggat ttcgccgtct tcgcgggcgt cctcccggag
     2881 ggcaagtaca ggctcgtgca ggcattccag cgcggcggtc acaccgtcgg catgtgcggc
     2941 gacggcgcca acgacgcgcc ggcgctgcgc caggctcaga tgggaatcgc cgtttcgact
     3001 gcgaccgacg ttgccaaatc ggcggccggc atggtgctca cccagcccgg actcgtcggc
     3061 atcgttgccg cagtcaggga aggccgggtc acgttccagc gcatccttac ctatacgctc
     3121 aacacagtca tcaagaagat tgtgacggtg ctctttctgg cggtgggcct ggtgatgacc
     3181 ggccatgcga tcctgacgcc aatgctgatg gtcatcatca tggtcacggg tgattttctc
     3241 acgatgtccc tgacgacgga caaggtacag gcgtcgccgc tgccgaatga gtggcggatc
     3301 ggcaacctga cgatcgcggg ggccgtcctg ggattttgcc tgctgggttt ttgcaccgcc
     3361 gtgctcgcgt tcggccattt cagcatggcg cttgggaccg cggcgctcaa tacgctggct
     3421 ctcgtagccc tggtattcgg cagccagacg gctctctatg cggtacgcga gcgccggcgc
     3481 ctctggtctc tccccggcct ctggctcaac gtatcgtccg cggccaatct tctcatcatc
     3541 tctacactgg cggtagccgg aatcctgatg acgccgttgc cgatagtggt ggtggccggc
     3601 acgcttggcg cggcggtggg cttcggattc gtgctgactg tggtgaagat tccggtattc
     3661 aggagactga ggatcgcgta gatccaggac gcaatggctg tcgctgaccc gtggtgccgt
     3721 aaaccgaaag gatgaacatg aaaatcgacc cggaagactt ccgcgtgcgg gaaggcgaca
     3781 aagtcaaact caagaagtgg ccgaccgtag tgaagccg
//
LOCUS       DATCPE010000065        12803 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U7219
            k141_43599, whole genome shotgun sequence.
ACCESSION   DATCPE010000065 DATCPE010000000
VERSION     DATCPE010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955033
            Sequence Read Archive: SRR1586279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 12803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 16:49:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 640
            CDSs (total)                      :: 631
            Genes (coding)                    :: 627
            CDSs (with protein)               :: 627
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12803
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43599"
                     /isolate="SMAG_U7219"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(25..282)
                     /locus_tag="VIM45_02000"
     CDS             complement(25..282)
                     /locus_tag="VIM45_02000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8820092.1"
                     /translation="MALARKYLPLNRHLEALDPGVESISITFSELETILGTPLPLSAW
                     LAHYWAGSSVARYNWERSGFAAKLDRLGRLVVFTRRPQRII"
     gene            447..1145
                     /gene="purQ"
                     /locus_tag="VIM45_02005"
     CDS             447..1145
                     /gene="purQ"
                     /locus_tag="VIM45_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HEY8820093.1"
                     /translation="MRFAIVRFPGTWSDVDCHHVVTEVLGQRADYVWHRETSLEGYDC
                     VVLPGGFAHGDYLRTGAVARFSPVMGAVAGFAASGRPVFGICNGFQVLCEAGLLPGAL
                     MRNDSLQFRCQWTNLRIESTDSPFTAACSPGQVLRIPISHGEGNYYADQATLDRLNDA
                     GRVAFRYSTPDGDTTPEANPNGSLENIAGIMSEGRNVLGMMPHPERAAEELTGGSDGL
                     VIWRSMIESFALSR"
     gene            1236..2033
                     /locus_tag="VIM45_02010"
     CDS             1236..2033
                     /locus_tag="VIM45_02010"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY8820094.1"
                     /translation="MSPAALALVFTAAMFHALWNRTLKIAGDRVATLAVADLIAGVIL
                     APAWIIAGPWSVLFLVFLSALAEAGYALCLSAAYVRGALSVAYPLGRGTAPLLVTLGG
                     WLVLSQPPGPLAVAGAVALAAGMALVATAGRRLGQIHAVGFALLAGVGIASYSVVDAR
                     AVSQVSAPGYLGVVLLITGIILTACLGRDQLRLLGSIKPGIQVAVGSTAAYLLILFAF
                     QRAHAGRVSTLREVSVLIGLWLSGERPAKRIWLGAMFVVGGIVLAAI"
     gene            2102..4429
                     /gene="purL"
                     /locus_tag="VIM45_02015"
     CDS             2102..4429
                     /gene="purL"
                     /locus_tag="VIM45_02015"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008413135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HEY8820095.1"
                     /translation="MTVEQRVLDQIALSRDEYELVVKRIGREPNDVELGMFGALWSEH
                     CGYKNSRPLLRLFPTEGSRVLTKAGEENAGAVEIGDGWLVAMKVESHNHPSAIEPLQG
                     AATGVGGIIRDIFAMGARPIAMLDSLRFGPLQPASDTPADVEEAAHNRHLFKEIVAGI
                     GWYGNCIGLPTVGGETGFSSSYNGNPLVNAMCVGIARVGELVKARADGPGNPLILVGA
                     DTGRDGIHGATFASVELNEQSEERRPAVQVGNPFMEKLLMEACLELVQTDWIVGLQDL
                     GAAGLTSAAIECASKSGTGLRIDVSRVPRREEGMTAYEVMLSESQERMLVIAKRGCEQ
                     KVQALFERWELSSDVIGEVTGDGMARIMDGGTEVACVPVPELTSPPQYIRQGIKPEWL
                     RDLGNFDLAALPDFATPTSRPPSTGEPEAKTTFGRQLTPAEAVFVRLLASENIASKRW
                     IYRQYDHQVMTNTVVGPGDGDAAVLRLKGTHRGIALATDCNGRFCFLDPYAGGAIAVA
                     EAARNVVCTGAEPVAVTDCLNFGNPEKPEVYFQLEQSIRGMAEACRVFSTPVISGNVS
                     LYNETNGSPIYPTPVVGMLGIIDDVRYRVGMGFGTAGDLVYLLGSGDGQGGLRGDAAA
                     LAGSEYLAVLHDTVAGRPTIDLDLEKRVQQVCLEAGRRGMISSAHDCSAGGLAVTLAE
                     SCCAGSVGIDATRMNISGRIDAGLFGESQSRIVVSLPPERAAELLELAASKNVPETLV
                     GRVGGANLRLADLIDLPVSGLAKVLDLALPRLLEG"
     gene            complement(4453..6738)
                     /locus_tag="VIM45_02020"
     CDS             complement(4453..6738)
                     /locus_tag="VIM45_02020"
                     /inference="COORDINATES: protein motif:HMM:NF019317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing metalloprotein"
                     /protein_id="HEY8820096.1"
                     /translation="MTFSSQRPQASVVLRAASFTVLLTLLLVAILFPILPSQLHIHQG
                     EIASQTIRAPRQFSYNSDVQRRQLQDQAVRNVPTAFSYDPNIQNTQLAKLGDVLSRIT
                     VIRQDAGANRAQAIDALNRLGVALTLDQRTAIAGYSDDEWKQAGDESMRLLSSVLEEP
                     FASTELAGRLASLPNRVSVGLTASQGDAAVGLARGFITPTERIDASATQRAQDQAIAA
                     VPPQPQAFAKDQVIVREGDVIDAFKLEALQSAGLLTVRLRYTDLGAAALLALVAAGVL
                     AAFVSHFRPDVLRATSRALLLGLIVAGIVALAKIYMPVVLPDSGHQFLPFAFPAAAVP
                     MLVTSLFDVELGVAVAGIAALLVTFTALYLPDLSGIVGLTALQPLEMACAFFFSGLAG
                     LLVVNRAERFNRFLSAGIAVAATTAIVLFGFWMLDTLHTLRDAGFLLMSSGINGIVSS
                     LLTVGTFVLLGSVFNISTRLQLMELAQLNQPLLRRLQEEAPGTFHHSILVGNLGERAA
                     DLIGADSLLVRVGCYYHDIGKLSRPAFFIENQLGGENPHDSLDPITSNHIVQEHVRFG
                     GELARKHHLPDRVRAFTVEHHGTRLVAFFYRKAAADDAQIDPALFTYPGPRPQSKETA
                     IAMLADSTEAVVRSSPDHGFEKIDELVEGVIAERLAEGQFDDCDLTLRDLRTIADSFK
                     ITLRAIYHPRIEYPAPTEAERQRTARRVALAPEPAELVPAASNGHLPEPIAEPATQQP
                     VHDSATVEPEDAETPNPIPMP"
     gene            complement(6802..7254)
                     /locus_tag="VIM45_02025"
     CDS             complement(6802..7254)
                     /locus_tag="VIM45_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEY8820097.1"
                     /translation="MSLKERLNDDLRTAMRGGDNQRRNTIRLALSSIRNAEIAARKPF
                     DEDGPVVDVLRKEVKQRRDSIDEFRKGKREDLVATEQAEMEVLLAYLPQTLDRAQISA
                     AAREVIAEVGATSVRDKGKVMGPLLKRLAGQAEGRDVNEVVSELLTGG"
     gene            complement(7289..7690)
                     /locus_tag="VIM45_02030"
     CDS             complement(7289..7690)
                     /locus_tag="VIM45_02030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8820098.1"
                     /translation="MGRRVDHVRFFIRGAVLAAVPLVAGLLLGAAHTLPATAQQPPTS
                     ITIIGAPVSLYTGCNTFVVRAPPGTSWRDIVARMATPNSVLGIWRYDNSSQRYEGVYF
                     SNSEAPTNGATSTIAFTFAIWTCVNANGSVS"
     gene            complement(7675..9174)
                     /locus_tag="VIM45_02035"
     CDS             complement(7675..9174)
                     /locus_tag="VIM45_02035"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY8820099.1"
                     /translation="MSIPLVLHNAEIHTMAPNQPRSTALLVRDGTIAAIGSDPDVRAE
                     SGPRFDDIDCGGDLVVPAFIDAHVHLLSYAASLVSVDVSPAAVGDLSSLLATVAARAE
                     TTPPGQWIRAYGYRETELTGRRHPTRWDLDSVAPRHPVRLIHGSGHGSVLNSMALQRV
                     AITNESEEPPGGFISRRLDDGDPDGLLLEMEHVLDGLIPRSEPEELHRGIAEANRRLA
                     ASGIACIQDLGVRNDASTLALFKQLHNDGTLSVEVVPALGIDAFLSDEVRGWTGLVKV
                     AVNELDGLRPDVATLSRWLYSIDKAGARAAVHAVSAGAIRHVLDAFEGAGVGWRRLSP
                     WHRIEHAAICPPDLAHRIAALGLVAVCNPGFVFEGGDRYLREVRTEDQPFLHNPSTLV
                     SAGAVVAAGSDAPVSAPDPVASIRASLVRRSSSGRRVPGKLLNLEAALALHTCSAAIA
                     GGQAASLGRLSKGETANLVRLPRALLEPVIRQTTAGKLRAMRAGAWVEG"
     gene            complement(9181..11145)
                     /gene="mnmG"
                     /locus_tag="VIM45_02040"
     CDS             complement(9181..11145)
                     /gene="mnmG"
                     /locus_tag="VIM45_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012191286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEY8820100.1"
                     /translation="MSTPPRHDRQPALYDVIVVGAGHAGCEAALAAARMGVRTLVLTM
                     NLETVALMPCNPSIGGPAKGHLVREIDALGGEMGRAIDRASIQIRMLNTGKGPAVQAL
                     RAQADKQLYMSEMRGVIERQPGLDIGRCLVESAVPAPAGDGKPAHYEVRGSQGELFRG
                     LRVILTTGTSLAGRMLTGENSVAGGRRGEGAATALSQSLRDLGIRLGRLKTGTPPRVH
                     ARTIDYSKTELQRGSETPLYFSFTPPSKVPEFPEPNTAYPGASLDGWRPQLPCYLVHT
                     NASTHDVIRANLHRAPMFNGSITGVGPRYCPSIEDKIVRFAEKESHGLFLEPEGWQTD
                     EVYVQGANTSLPEDVQLAMLRTIAALEHCEMVRVGYAVEYDYVVTDQTRVTLESKSLP
                     GLYFAGQINGTSGYEEAAAQGLMAGINAAHAVQGRAPLVLRRDQAYIGVMLDDLVSQD
                     LREPYRLFTSRAEYRLLLRQDNADMRLSPIGYKIGLIDGPRARRVEEKREAVEREAAR
                     LNGEFLSHTAEMQQRADALGLGALRQGLTAGDVLRRPHVGYNALLALGVGDESLARDA
                     VEQVEIELKYRGYLERQEREVERVRRYDGRPLPDQLDYESLRGLRTEAREKLARFRPA
                     TVGQAGRLAGVTPADVAVLLVHLERLSVPA"
     gene            complement(11382..11561)
                     /locus_tag="VIM45_02045"
     CDS             complement(11382..11561)
                     /locus_tag="VIM45_02045"
                     /inference="COORDINATES: protein motif:HMM:NF018140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8820101.1"
                     /translation="MNSRFRGSYLRVIFKERSNRVANQVGKRYQCTKCNTEMIVTKAG
                     NGSLGCCGQPMQQKQ"
     regulatory      11705..11814
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            11905..>12803
                     /gene="thrC"
                     /locus_tag="VIM45_02050"
     CDS             11905..>12803
                     /gene="thrC"
                     /locus_tag="VIM45_02050"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009371447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEY8820102.1"
                     /translation="MTFSRSLRCRECSREYPLQALHVCEFCFGPLEVAYDYEAIAAST
                     SRRRIARGPNTLWRYVDFLPVDGESRIDLHAGFTPLVRSKNLADRLGLRELYIKNDTV
                     NPTWSFKDRVVAVASSRARELGYTTLACASTGNLANSVAAHAARAGMDAYVFIPSDLE
                     QGKVLGSAIYNPTLVAVDGNYDEVNRLCAELSGRYPWAFVNINVRPYYSEGSKTLGFE
                     TIEQLGWRSPDHCVAPIASGSLFTKIYKGMKEFEKVGLIDSVHTRMSGAQADGCSPVA
                     TAFREGSMNFRPVKPKTIAKSLA"
BASE COUNT         2338 a         3939 c         4126 g         2400 t
ORIGIN      
        1 aaataacagg ccggctccgg tcggttagat gatccgttgg gggcgccgcg tgaagacgac
       61 gagccgccca aggcgatcaa gttttgcagc gaagccgctg cgctcccagt tgtagcgggc
      121 aaccgagctt ccggcccagt aatgtgcaag ccaggcgctg agtggcagcg gcgtacccag
      181 aatcgtctcg agttcggaaa acgtgatcga aatcgactct acgccggggt ccagcgcttc
      241 caggtgtcgg ttgagtggca aatacttacg ggcgagcgcc atggtggtgg gtggtcctca
      301 aaatcttgcg gatctcgctc tcgattgatc gagtgtactc cctcgatcgg tgccgatgcg
      361 cctgtcgccg gcttgtcgcc ggtgaccccg acacgggaca atgagtccaa gagtcagcaa
      421 tagcgacgcc aagttgaggt gttcgtgtgc gctttgcgat cgtacgtttt cccggcactt
      481 ggtcggacgt cgactgtcat cacgtggtga ccgaggtcct cggacagcgg gcggactacg
      541 tctggcaccg cgaaacgagc ctcgaagggt atgactgcgt cgttttgccg ggcggcttcg
      601 cgcacggtga ttacctgcga acgggagccg tcgcacgttt ctcccccgtc atgggcgcgg
      661 tggccggctt tgcggcgagt ggccgcccgg tgtttggtat ctgcaacggc ttccaggtgc
      721 tgtgcgaagc gggactgttg cccggcgcgc tcatgcgcaa cgacagcctg cagttcaggt
      781 gccagtggac gaatttgcgc atcgaaagta cggattcgcc atttacggcg gcatgctcgc
      841 ccggccaggt cctgcgtatc ccgatctcgc atggcgaagg caattactat gccgatcagg
      901 cgacactcga ccgcttgaac gatgccgggc gcgtcgcatt tcggtacagt acgccggatg
      961 gggataccac tcccgaggcc aacccgaatg gttcgctgga gaacatcgcc ggcatcatga
     1021 gcgagggccg aaacgtcctc gggatgatgc cgcacccgga acgggcggcc gaagagctga
     1081 ccggtggaag cgacggcctc gtgatttggc gttcgatgat cgagtcgttt gcgctcagtc
     1141 gataagccgg ctaatcgtgg caactgataa gcccgtggcg ggaggccgct cgcgtgatgc
     1201 acccgccccg ccgggcagag taggagcgcc gccagatgtc gccggcagcg ctcgcgctgg
     1261 tcttcaccgc ggcgatgttc catgccctct ggaatcgcac ccttaaaatt gccggcgatc
     1321 gcgttgccac ccttgcggtt gccgatctaa tcgccggcgt catcctcgcg cccgcgtgga
     1381 tcatcgccgg gccctggagc gtgttgttcc tggtattcct ctccgcgctc gctgaggcag
     1441 gctacgctct ttgcctctcg gcggcgtacg tccgtggcgc tctgtcagtc gcgtatcctc
     1501 tcgggcgcgg gacggcgccg ctgctggtaa cgctcggcgg ttggctcgtc ctttcgcaac
     1561 ctccgggacc gcttgccgtg gccggggcgg tcgctctggc ggccggcatg gcgctcgtgg
     1621 cgacggcggg gcggcggctc gggcagattc acgcggtcgg ctttgcgctg ctggccgggg
     1681 tcggaatcgc cagctattca gtggtcgatg cccgggcggt aagccaggtt tcggcgcccg
     1741 gatatctggg cgtcgtgttg ctaattaccg gcatcatcct tacagcctgc ctgggccgtg
     1801 accagctccg gttgttgggc tcaatcaagc cggggatcca ggttgcggtc ggttcgaccg
     1861 ccgcctacct gctgatcctc tttgcttttc agcgggcaca tgcgggccga gtctcgactt
     1921 tgcgtgaagt gtcggtgttg atcggcctct ggctctcggg cgaacggccg gcgaagcgga
     1981 tctggttggg ggcaatgttc gtggtgggcg gcatcgtgct ggcggctatc tgatgccatc
     2041 tgcaatttca cctggcgggc tccgattgtg gctcgtcgta gcggcatagt acgctccggt
     2101 catgaccgtc gaacaacgtg tcctcgacca aattgcactc tcgcgcgacg aatatgagct
     2161 cgtggtcaag cggattggcc gcgaaccgaa cgacgtagag ctaggcatgt ttggcgccct
     2221 gtggagcgag cactgcggct acaagaacag ccgcccgcta ttgcgcctct tcccaaccga
     2281 aggcagccgt gtgctgacca aggcgggaga agagaacgct ggcgctgtag aaataggcga
     2341 cggctggctg gtggcgatga aggtggagtc gcacaaccat ccgtctgcga tcgaaccgct
     2401 gcagggcgcc gccaccggcg tgggcggcat tattcgtgac atcttcgcga tgggcgcccg
     2461 gccgatcgcc atgctcgact cgctgcgctt cggaccgctc cagccagcct cggatacgcc
     2521 cgcggacgtg gaggaggccg cgcacaaccg gcatctattc aaagagatcg tggccggcat
     2581 cggctggtac ggcaactgta tcggactgcc gaccgtgggg ggcgaaacag gattttcttc
     2641 gtcgtacaac ggtaatccgc tggtgaatgc gatgtgtgtt gggatcgcgc gcgtcggcga
     2701 gctcgtgaag gcgcgcgccg acggtccggg caatcctctg atcctcgtgg gcgccgacac
     2761 cggacgcgac ggtatccacg gagcgacctt cgcaagcgtc gagttgaacg agcagtccga
     2821 agagcgacgt cctgcggtgc aggtcggcaa cccgttcatg gaaaaactgc tgatggaggc
     2881 ttgcctcgag ctcgtgcaga ccgactggat cgttgggttg caagaccttg gcgctgccgg
     2941 gctgacaagc gccgcgatcg aatgcgcctc gaagtcggga acgggtctgc ggatagacgt
     3001 gtcacgtgta ccgcgccgcg aggagggaat gactgcgtac gaagtcatgc tctccgaatc
     3061 gcaagagcgc atgctggtca ttgcaaaacg cgggtgcgag caaaaggtcc aggcgctgtt
     3121 cgagcgctgg gagctgagtt ccgacgtgat cggtgaagtt accggcgacg gcatggcccg
     3181 catcatggac ggcggtactg aggtcgcgtg cgtgcccgta cccgaactca caagcccgcc
     3241 gcaatacata cgacagggca tcaagcccga gtggttgcgc gacctgggga actttgacct
     3301 tgccgcgttg cccgacttcg cgacgccgac gagccggcca ccgtccacag gggagccgga
     3361 agcaaagacg accttcgggc gacagctgac gcccgcggag gcagtattcg tccggttgct
     3421 tgcttcggag aacatcgcct caaagcgctg gatctaccgc cagtatgacc accaggtgat
     3481 gacaaacacc gtcgtgggac ccggagatgg cgacgcggcg gtgttgcggc tgaagggcac
     3541 ccatcgcggc atcgcactcg cgacagactg caacggccgt ttctgcttcc tcgacccgta
     3601 cgctggcggc gcgatcgccg ttgccgaagc cgcgcgcaac gtcgtctgca ccggagccga
     3661 accggtcgcc gtgaccgact gcctgaactt cggcaacccg gaaaaaccgg aggtgtattt
     3721 ccaactggag cagtcgattc gcggcatggc cgaggcctgt cgcgtattct ccacgccggt
     3781 gatatccggc aacgtgagcc tttacaacga gaccaacggc agcccgatct atccgactcc
     3841 ggtcgtcggc atgctgggca tcatcgacga cgttcgttac cgggtcggca tgggctttgg
     3901 cacggccggc gacctggtgt acctgctcgg gtcgggagac ggccagggag gcctgcgagg
     3961 agatgccgcg gcactcgcgg gcagcgagta cctggcggtt ctgcacgaca ccgtcgccgg
     4021 ccgccccacg atcgatctgg atctggagaa gcgggtccaa caggtatgcc tggaagcggg
     4081 ccggcgaggg atgatatctt cggcgcacga ctgctccgcc ggcggactgg ccgtgacgct
     4141 cgccgaaagc tgttgcgccg gcagcgttgg aattgatgcg acgcgtatga acatcagcgg
     4201 tcgtatcgac gcaggccttt ttggggagag tcagtcccgc atcgtcgtct cactcccacc
     4261 cgagcgagct gcggagcttc tggaattggc tgcctcaaag aacgtgccgg agaccctcgt
     4321 gggccgggta ggcggcgcca atctgcgcct tgctgatctg attgatctcc cggtctccgg
     4381 ccttgcgaag gtcttggacc tggcactgcc gcgcttgctg gaggggtgag agcggcgtgc
     4441 tcgaacccgg tattatggca tcggaatggg attaggcgtc tccgcgtctt cgggctcgac
     4501 cgtggccgaa tcgtgaaccg gctgttgtgt cgccggctcg gcaatcggtt ccggaagatg
     4561 cccgttcgac gccgccggca caagctcagc cggttccgga gcgagggcca cgcggcgcgc
     4621 ggtgcgttgt cgctcggcct ccgtgggtgc ggggtactcg atacggggat ggtaaatagc
     4681 gcgcagcgtt attttgaaag agtcagcgat cgtgcgtagg tcgcgcagcg ttaggtcaca
     4741 atcgtcaaat tgcccctccg ccaaccgctc cgcgatcacg ccttccacga gctcgtctat
     4801 cttctcaaag ccgtggtccg ggctgctcct gacgaccgct tcggtcgaat cggcaagcat
     4861 ggcaatcgcc gtctctttgc tctgcggacg cggccccgga taggtgaaca acgccggatc
     4921 gatctgcgcg tcgtctgccg cggccttgcg ataaaagaac gccacaagcc gcgtgccgtg
     4981 atgttcgact gtgaaggccc gcactcggtc cggcagatga tgcttgcgcg ccagctcacc
     5041 cccgaaacgg acgtgctcct ggacgatgtg attgctggta attgggtcga gcgagtcgtg
     5101 cggattctcg ccgcctaact ggttttcgat aaaaaaggcg gggcgcgata gcttgccgat
     5161 atcgtggtag taacagccga cgcggaccag cagcgagtca gcgccgatga ggtcggcagc
     5221 gcgctcacca aggttgccga ccaggatgct gtggtgaaag gtgccgggcg cctcttcctg
     5281 cagccggcgt aacagcggct ggttcaactg ggcaagctcc atgagctgca ggcgggtgct
     5341 gatgttgaaa acgctgccga gcagaacgaa ggtgccgacc gtgaggagcg agcttacaat
     5401 tccgttgatc ccactggaca taagcaaaaa tcccgcatcg cggagggtat gcagcgtgtc
     5461 gagcatccag aaaccgaaca aaacgatcgc ggtggtggca gcgacggcaa ttcccgcgct
     5521 gaggaagcgg ttgaaacgct cggcccggtt cacgaccagc aggccggcga ggcccgaaaa
     5581 gaagaacgcg cacgccatct ccagcggctg cagcgcggta agtccgacga ttcccgagag
     5641 atccggcaaa tacagcgccg tgaacgttac cagcagggca gcaatgccgg cgacagctac
     5701 gccaagctcg acgtcgaaca acgacgtgac cagcatgggc accgcggccg cgggaaatgc
     5761 gaacggcaaa aactgatgcc ccgagtcggg aaggacgacc ggcatgtaga tcttcgccag
     5821 cgccacgatt cccgcgacga tgagcccgag caacagggca cgcgacgtgg cccgaagcac
     5881 gtcggggcgg aaatgcgaga cgaacgcggc caggacaccg gcggccacga gcgccagcaa
     5941 ggcggcagcg ccaagatccg tgtatcgcag ccgcacggtg agcaggccgg cgctttgcaa
     6001 cgcctccagt ttaaaagcgt cgatcacgtc gccttcgcga acgattacct ggtctttggc
     6061 gaaagcctgt ggttggggcg gcaccgccgc gatcgcctgg tcctgggctc tttgcgtggc
     6121 ggacgcgtcg atccgctcgg tcggggtgat aaatccgcga gccaacccga ccgctgcgtc
     6181 gccctggctc gcggtcaatc caacgctgac acggttcgga aggctcgcaa ggcgccccgc
     6241 cagctcggtc gaagcgaatg gctcttccag gacgctcgat agcaaccgca tcgattcgtc
     6301 gcccgcttgc ttccattcat cgtcggaata tccggcgatg gccgtacgct gatccagcgt
     6361 cagcgcaacg cccagccggt tgagggcgtc gattgcctgg gcacgattgg cgccggcatc
     6421 ctggcggatg accgtgatgc ggctgagtac gtcgccgagc ttggcgagtt gggtgttctg
     6481 gatgtttggg tcgtacgaga acgccgttgg gacgtttcgg accgcctggt cctgcagttg
     6541 gcggcgttgg acgtcgctgt tatacgagaa ctggcgcggc gcccggatcg tttgcgaagc
     6601 gatttcaccc tggtgaatgt gcagctggct cggcaagata gggaaaagga tagcgaccaa
     6661 caacagggtc aacagcacgg tgaagctggc ggcgcgtaga acgacacttg cttgtggtcg
     6721 ttgcgaagag aaagtcactg actgaacacc atgtgcgggc cgtcgggaca acggccatcg
     6781 gtcatcatat cagagctcac gtcagccgcc ggtgagcagt tcgctcacga cttcgttgac
     6841 gtcacggcct tcggcttgcc cggccagccg cttcagcaac ggccccatca ctttgccttt
     6901 gtcgcgaaca ctggtcgccc cgacttctgc gatcacttcg cgagcagcgg ccgagatctg
     6961 cgctcgatca agcgtctgcg gcaaatacgc aagtagcacc tccatttcgg cttgttctgt
     7021 tgcaaccagg tcctcgcgct tgccctttct gaactcgtcg atcgaatcgc ggcgctgttt
     7081 cacttctttg cgaagaacgt ccacgacggg tccgtcttcg tcgaatggct ttcgggccgc
     7141 gatctcggcg ttccggatcg aagagagggc aagccggatc gtgtttcggc gctgattgtc
     7201 gccgccgcgc attgcagtcc tcaagtcgtc gttcagccgt tctttgagcg acattcaggt
     7261 gctcctcgtt accaaagccg gtcgagcttt aactgaccga cccattcgcg ttgacgcacg
     7321 tccatatcgc gaacgtgaac gcaatcgtac tcgtggcgcc gttggttgga gcctctgagt
     7381 tgctgaaata gacgccctca tagcgctgtg aggagttatc ataccgccat attccgagga
     7441 cgctgttggg ggtcgccata cgggcgacga tgtcgcgcca cgaggtgccc ggcggcgcgc
     7501 gcacgacgaa ggtgttgcat cccgtgtaga ggctgaccgg ggcgccgatg atcgtgatcg
     7561 acgtcggcgg ctgttgagcg gtcgcgggca gcgtgtgcgc cgcgcccagc agcaatccgg
     7621 caaccagcgg aacggccgca aggacagcgc cccgaatgaa aaatcgcacg tggtctaccc
     7681 ttctacccaa gcgccggccc gcattgcgcg gagtttgccg gcggtcgtct gtcgaataac
     7741 cggttccaga agcgctcggg gcagccgcac gagattcgcc gtctcgcctt tcgatagccg
     7801 tccaaggctt gccgcttgac ctccagcgat cgccgcgctg caggtatgca acgccagagc
     7861 cgcttcgagg ttcaggagct tgccggggac gcgccgcccc gaactggaac gccgcactaa
     7921 cgacgctcgt atagatgcta cgggatcggg agcgctgacc ggagcatcgg agccggcggc
     7981 gactactgcc ccggccgaga cgagcgtcga cgggttgtgc aggaacggct ggtcctccgt
     8041 tcgaacctcg cgtaaatacc gatcgccgcc ctcgaacacg aagcccggat tacagacggc
     8101 aaccaggccg agcgccgcga tgcgatgggc aaggtcgggc ggacagatag cggcatgttc
     8161 gatgcggtgc cacggtgaca accgccgcca accaactcct gccccctcaa atgcgtccaa
     8221 gacgtggcgg atggcgcctg cgctcaccgc atgaacggcg gcgcgggccc ccgctttatc
     8281 gatcgaataa agccacctcg aaagagtcgc aacatcgggt cttaagccgt ctagttcgtt
     8341 gactgcgacc tttactaatc cggtccaacc acgcacttcg tcgctgagga aggcatcgat
     8401 ccccagcgcg ggcactactt cgacagataa tgtgccgtcg ttatggagct gcttgaacaa
     8461 ggccagcgtc gaagcgtcgt tgcggacgcc caaatcctgg atgcatgcga tgccgctggc
     8521 ggccaggcga cggtttgcct cggcaatgcc gcgatgcagc tcctcgggct cagagcgcgg
     8581 aattagccca tccagcacat gttccatctc gagtagcaag ccgtcggggt cgccatcatc
     8641 caatcgcctg gagataaacc cacccggtgg ctcctctgac tcgttggtaa tcgcgacacg
     8701 ctgaagcgcc atcgaattca gtacggaccc gtggccggac ccgtgaatca agcgtaccgg
     8761 gtgcctcggc gcgacactat ccagatccca acgcgtcgga tgacggcggc cggtgagctc
     8821 ggtttcgcgg tagccgtagg cgcggatcca ctgccccggc ggcgttgtct ctgcgcgcgc
     8881 tgctacggtt gcgagcaggc tactcagatc gcccacggcc gcaggagaaa cgtccacgga
     8941 gaccagcgaa gctgcatagc tgagcagatg gacgtgggcg tcaataaacg cgggcaccac
     9001 gaggtcgcca ccgcaatcga tatcgtcaaa gcgtggccct gattcggctc gaacgtccgg
     9061 gtcggacccg atcgccgcga ttgtgccgtc gcgtaccagc agcgccgtcg agcgcggttg
     9121 attaggagcc atcgtgtgga tctcggcgtt gtgtaggacc aggggaatcg acacggcgag
     9181 ctacgccggt accgatagcc gctcgaggtg gaccagcagc accgcgacat cggcgggcgt
     9241 gacaccagcc aggcgcccgg cctgacccac tgttgccggt cggaaccggg cgagcttctc
     9301 gcgcgcctcg gtgcgtaacc ctcgaagact ttcgtagtcc agctgatcgg gcagcggccg
     9361 gccatcgtaa cggcgcaccc gttcgacctc tcgttcctgg cgctccaggt agccgcggta
     9421 cttgagctca atctctacct gctcgaccgc gtcgcgagca agggactcgt cgccaacgcc
     9481 taaggccagg agcgcgttgt agccaacgtg cgggcggcgg agcacatctc cggccgtcag
     9541 tccctggcgc agcgccccaa ggccgagcgc atccgctcgc tgctgcattt cggccgtatg
     9601 ggataggaac tcgccgttga gtcttgcggc ctcccgctcc acggcctcgc gcttttcttc
     9661 gacacgccgc gcacgtggac cgtcaattaa tccgatcttg tagccgattg gcgacagacg
     9721 catatcggcg ttgtcctgtc gcagaagcag ccggtattcg gcgcgcgacg taaacagccg
     9781 gtacggctcc cgtagatcct gcgagacgag gtcgtcgagc attacgccga tgtacgcctg
     9841 gtcacgccgc aaaaccagcg gcgctcggcc ctgaaccgca tgcgcggcgt tgatgccggc
     9901 catcaagccc tgtgccgccg cctcttcgta gccggaggtg ccgttgatct gtccggcaaa
     9961 gtagaggcca gggagagatt tagactcaag cgtcacgcgc gtctggtcag tgacgacgta
    10021 gtcgtattca accgcgtagc caacgcggac catttcgcag tgttcgagtg ccgcaatggt
    10081 gcgcagcatc gcgagttgta cgtcttccgg caggctggtg ttcgcgccct gcacgtagac
    10141 ttcgtccgtc tgccatccct cgggctccag gaacaggcca tgcgactctt tttcggcaaa
    10201 gcgaacaatc ttgtcttcga tcgacgggca gtatcgcggt ccgacgccgg tgatcgaccc
    10261 gttgaacatc ggcgcgcgat gcagattggc gcggatcacg tcgtgtgtgg aagcgttcgt
    10321 gtgcacgagg taacagggca attgtggacg ccacccatcg aggctggcac ccggataggc
    10381 cgtgttcggc tccggaaact ctggcacttt cgagggcggc gtgaagctga aatacagcgg
    10441 cgtttcactg ccgcgctgca gctctgtctt gctgtagtct atcgttcgcg cgtgcacgcg
    10501 tgggggcgtg cccgttttga gacggccaag gcgaattccg aggtctcgca ggctctgcga
    10561 aagggcagta gcggccccct cgccacgtct ccctcccgct acagagttct caccggtgag
    10621 catgcggccg gcgagcgaag tgcccgtcgt caggatcacc cgaaggccac gaaagagctc
    10681 gccttgagag ccccgaacct catagtgagc gggtttgcca tcccctgccg gcgcgggtac
    10741 ggccgactcc acgaggcagc ggccgatgtc aaggcctggt tgccgttcga tgaccccccg
    10801 catctcgctc atgtagagct gcttgtcggc ctgcgcccgc agggcctgga cagccggacc
    10861 cttgccggta ttcaacatgc ggatctggat ggacgcgcgg tcgatcgctc ggcccatttc
    10921 gcccccaagc gcgtcgatct cgcggacaag gtggcccttt gccggtcctc caatcgacgg
    10981 gttgcacggc attagcgcca cggtttcgag gttcatggtc aggacgaggg tacggacgcc
    11041 catccgagcc gcagcgagag cggcctcgca tccggcgtgg ccggcgccca cgacgatgac
    11101 gtcatatagg gctggttgtc ggtcgtgacg cggcggcgtc gacatggcat cactcatgaa
    11161 gctgggggta tgacgaagcc gcgggggctg ggccgcccgc ggccgcattg ccggacctgt
    11221 agttgcacta gtcggccgac ggaaggacct tcggctgaag caaggtcatc gccttctcgc
    11281 agcactgtac tgcgccgtcg ccgggcttga tacagagaac ttccgtcccg cagtcctcac
    11341 aatggaaacg cttaccgagc tggttcgcca tcctacctca cctactgctt ttgctgcatt
    11401 ggctggccgc agcacccgag actaccgttg cccgctttgg tcacgatcat ctccgtgttg
    11461 cactttgtgc attggtaccg tttgcctacc tgattcgcca cacgattgct ccgctctttg
    11521 aaaatcactc gaagataact gccgcggaac cgtgaattca cgcataaatc cgccgcccac
    11581 tcggccaaag tctagcccgt gattgcagct tgagtcaacg cggggaaggc catgcgaatg
    11641 gtcgtctatt gacgagcgat tcaggcgtct cgtacaatat tagctaacaa ttgaatatcg
    11701 ggtcctctta tcaagagagg tggagggaaa cggccctacg aagcccggca accgagcccg
    11761 ttttacggga agcggtgcca actccggcct ggtacacgcc aggcgagata agagacgaag
    11821 taccgctttg gtacggacgc ctcacaccca cctcttctcc acccatagga gaagaggttt
    11881 tttcatgcct agggggacgc agccatgacc ttttcccgat cactccgctg tcgcgaatgt
    11941 agccgcgaat atccgctgca agcgctgcac gtctgcgagt tttgctttgg cccgctcgag
    12001 gtggcgtacg actatgaggc gatcgccgcc tcaacgagca ggaggcgcat tgcgcgtgga
    12061 ccaaatacgt tgtggcgcta cgtcgatttc ttgccggtgg atggcgagtc gcggattgac
    12121 ttgcacgcgg ggttcacgcc gctcgtccgc agcaagaacc tcgcagaccg attgggcctg
    12181 cgcgagctct acataaagaa cgacacggtg aaccccacgt ggtcgttcaa ggaccgcgtc
    12241 gtagccgtcg ccagttcgcg agcgcgggag ctcggataca cgacattggc gtgcgcctcg
    12301 acgggtaacc tcgctaatag tgttgcggca cacgcggcgc gggctggtat ggacgcatat
    12361 gtgttcattc cgtcggatct ggagcagggc aaagtgctgg gctcggcgat ctacaacccg
    12421 acgctggtgg ccgtcgacgg caactacgat gaggtcaatc gactctgcgc ggagttgagc
    12481 ggccgatacc cctgggcatt cgttaacatc aacgtgcgcc cgtattactc cgaaggcagc
    12541 aagacgcttg gattcgaaac gatagagcag ctcggctggc gctctccgga ccactgcgtg
    12601 gcaccgatcg ccagcggttc gttgttcacc aagatttata agggcatgaa agagttcgag
    12661 aaggtcggtc tcatcgacag cgtgcataca cgcatgtcag gtgcccaggc cgacgggtgc
    12721 tcgccggtag ccacggcctt tcgcgaaggc tcaatgaatt ttcgcccggt gaagccgaag
    12781 acgatcgcca agtcgcttgc gat
//
LOCUS       DATCPE010000066         6099 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U7219
            k141_595126, whole genome shotgun sequence.
ACCESSION   DATCPE010000066 DATCPE010000000
VERSION     DATCPE010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955033
            Sequence Read Archive: SRR1586279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 6099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 16:49:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 640
            CDSs (total)                      :: 631
            Genes (coding)                    :: 627
            CDSs (with protein)               :: 627
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6099
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_595126"
                     /isolate="SMAG_U7219"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(140..1348)
                     /gene="argJ"
                     /locus_tag="VIM45_02055"
     CDS             complement(140..1348)
                     /gene="argJ"
                     /locus_tag="VIM45_02055"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HEY8820103.1"
                     /translation="MPKDIPNGSVTSPAGFLAGVAVAGVKHADSTRPDVGLLFSVRPC
                     TAAGMFTRSTVEGAPVTVSREYLANGRAQAIVANSGIANVAMGQTGLRDARDMAAEAA
                     AKLGIRQDDVIVASTGVIGWPLPMDRIRKGIRQVEVRTDGGIDLARAIMTTDTRPKHV
                     AVEFEASGRTYRAGGIAKGSGMIHPDMGTMFAFLTTDAPVEGACLKMLMRSVVDETFN
                     MVSIDGDTSTSDTVVLLANGAAGGEPIRSDTPAADSFREAIYSVSRRLAREIAADGEG
                     ASKLIEVRISGARTLEEARKAARTITVSPLMKSAVYGSDFNWGRIMMAIGRSGAAIEM
                     ERAEVSIGDVVAYTRGAAASVDPAAARSALQGPEVTISANLGTGDASAVAWGCDLTEE
                     YVKINAEYTT"
     gene            complement(1417..2448)
                     /gene="argC"
                     /locus_tag="VIM45_02060"
     CDS             complement(1417..2448)
                     /gene="argC"
                     /locus_tag="VIM45_02060"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEY8820104.1"
                     /translation="MIRVGVLNVTGYAGVEAARLLLQHPNATMVAATGRSEAGKPLAA
                     VFPHLWRYDLTITPEIDTDVDAVISCLPHAASAAALLPFVERGVPVIDVSADFRLRDP
                     DEYREWYGVEHPSPEALKFAVYGLPELHREAIRKSRLVANPGCYPTAAILALAPAFKA
                     GIVEPGVIVDSKSGISGAGRGAKLEYNYSEVDEDFSAYGLNGHRHLPEITQELAAFTC
                     NTPPRVTFVPHLVPMIRGIETTCYADLRLDRLGSDPQSELRGIYRDFYADAPFTHVTD
                     SAPHTKHTAGSNYCHVFPTVDLRTGRLVVSACLDNLVKGASGQAIENMNLMLGLPQTA
                     GIDSPALYP"
     gene            2642..3583
                     /locus_tag="VIM45_02065"
     CDS             2642..3583
                     /locus_tag="VIM45_02065"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012881706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEY8820105.1"
                     /translation="MYQELSIFTGNAHPALAQAICDRLGRPLGQCAVFEFSNENIFVQ
                     IEENVRNRDVFIIQPAASPVSTRLMELFIMLDAFRRASAGRISVVMPYYAYGRSDKKD
                     QPRVPITARLIANILETAGADRLLTVDLHAGQIQGFFNVPVDELSTLHSFEMYFRELD
                     LPDPVVVATDLGASKRARNLAERLEVPLAVMEKRRAANDDTAEIMTVIGEVNGKTALI
                     VDDEVDTAGTLIQAVCALHERGAKAIYAAATHGVLSGPAIKRIDDSPLQQLVVSDSLP
                     TPSGKQSDKIRTLSLAPMLADAITRIHTGQSVGAMFH"
     gene            3683..>6099
                     /gene="secA"
                     /locus_tag="VIM45_02070"
     CDS             3683..>6099
                     /gene="secA"
                     /locus_tag="VIM45_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEY8820106.1"
                     /translation="MKFLSKLIGDPNEKEIKHLLPIVEEVNALEPETQALSDEELRAK
                     TDEFKQRLADGETLDDLLPEAFAVVREASHRYTGRRHFDVQIMGGVILHRGQIAEMKT
                     GEGKTLVATLPLYLNALTGLGVHLVTVNDYLAKRDPQYMGPGTIFTRLGLSVGVLQHE
                     AAFLVSHDKVSDQPNAEYLVPCSRNEAYLADVLYGTNHEFGFDYLRDNMSRSLDGCVQ
                     RELSYAIVDEVDNILIDEARTPLIISGPAEENVQIYQAFARVVPTLSLNTDYEVDEKQ
                     RVVTLTDEGVERIQRGLGIGNIYSAENFKLTRFLDAALKAHIIYQRDRDYVVKDGEVI
                     IVDDFTGRLMHGRRWSDGLHQAVEAKERVKIQNESVTYATITLQNYFRLYEKLAGMTG
                     TAMTEAEEFHKIYKLEVVSVPTNQPMVRVDNGDLVYRTEEAKFAAVASEVMERSQRGQ
                     PVLVGTVSIEKSEYLAELLRRRGVKLEVLNAKQHEREASIVAQAGRLGGVTIATNMAG
                     RGTDIILGGKPDERDDEEWKNEHNNVVEQGGLHIIGTERHESRRIDNQLRGRAGRQGD
                     PGSSRFYVSFEDDVMRRFAPDWLPGMMTKLGMDDETPIEHSWVSKAIHTAQSKVEGYN
                     FDIRKHVVEYDDVMNMHRDVIYRERRKILEGADVRENVRTMSEEEVGAVVSAHVDDRP
                     ADEWDLESLMAQINAIMELPPGLDQATLIEMTPADIKEQLTDHARQLYDAKIAQFERV
                     RGIDPFGDDPFGFVERQVLLQTIDALWIEHLTAMEEMREGIGLQAYGQNDPLVMYKRE
                     AHDMFEQL"
BASE COUNT         1278 a         1837 c         1858 g         1126 t
ORIGIN      
        1 atagggacac cgcggcggtg caacgcggcg atgtcgctca acgtcgtgtc gtgggcgccg
       61 agcgtgctgc cgccgagctt tactacaacg gtttggttga attcgggcac cctgcgaggc
      121 tcccagacga aatcgcgact caggtcgtgt actccgcgtt gatcttcacg tactcttctg
      181 tgagatcgca gccccaagcg actgctgacg cgtcacctgt gcccaggttc gcgctaatag
      241 tgacttcggg accctggagc gccgacctgg ccgcggcggg gtccacgctt gcggccgcgc
      301 cgcgcgtata ggctaccacg tcgccgatgc tcacttcggc tcgttccatc tcaattgccg
      361 cgccgctgcg gccgatcgcc atcataatcc tgccccagtt gaagtcgctt ccatagaccg
      421 ccgacttcat cagcggcgag accgtgattg tccgtgcggc cttccgcgcc tcttcgagcg
      481 tcctggcgcc ggaaattcgg acttcgatca gcttgctcgc tccttcgccg tcggcggcga
      541 tctcgcgcgc cagccggcga gagacggaat aaatcgcctc tcgaaaactg tcggccgcgg
      601 gtgtatcgga ccgaatcggc tctccccccg cggcgccatt cgccagaagc actacagtgt
      661 cgctggtgct tgtgtcgccg tcgatgctga ccatgttgaa cgtctcgtcc acgaccgaac
      721 gcatgagcat tttcagacag gcgccctcga ccggggcgtc ggttgtgaga aaggcgaaca
      781 tcgtacccat atccgggtgg atcatgcccg aacctttggc tatgccgccc gcgcggtacg
      841 tcctaccgct cgcctcgaat tcaacggcca cgtgcttcgg ccgcgtatcc gtcgtcatga
      901 tcgcgcgggc caggtcgatg ccgccatcgg tcctgacctc aacctgccgt atccctttcc
      961 gaatgcggtc catcggcagc ggccagccga tcacgcccgt agacgccaca atcacatcgt
     1021 cctggcggat cccgagcttc gcggcagcct ccgctgccat atcgcgcgcg tctcgcagcc
     1081 ccgtttgccc catcgccacg ttcgcgatac ccgagtttgc gacgatggcc tgtgcgcggc
     1141 cgtttgcgag gtattcccgt gacaccgtga ccggagcgcc ctcgacagtg ctgcgcgtga
     1201 acatgccggc ggcggtacag ggccgaactg agaacaacaa tccgacgtcg ggtcgcgttg
     1261 aatcagcgtg tttgacgccg gcaacagcca caccggcgag gaatccggcc ggcgaggtga
     1321 cgctgccgtt tgggatatct tttggcatgg cgcgggaaga cctcctcttg caggtctggc
     1381 cggttatcga tggcccggga gggtggctga agggcctcag gggtacagcg ctggactatc
     1441 gattccggcc gtttgtggca gaccgagcat gaggttcatg ttctcgatcg cctgtcccga
     1501 cgcgcccttt accaggttgt ccaggcacgc cgagacgaca agtcgcccgg ttcgtagatc
     1561 aaccgtcggg aaaacgtggc agtagttgct tccagcggtg tgcttggtgt ggggcgccga
     1621 atcggtcacg tgggtgaacg gcgcatccgc gtagaagtcg cggtagatgc cgcgaagctc
     1681 agactgtggg tcggaaccga gtcggtcgag ccggaggtcc gcgtagcacg tggtttcaat
     1741 gccgcgaatc attggcacta ggtgcggcac aaacgtcact cttggcggcg tgttacaggt
     1801 gaacgcagcg agttcctgag tgatctcggg aagatgccga tggccgttga gcccataggc
     1861 cgaaaaatcc tcgtcgactt cgctgtagtt gtactcaagt ttcgcgccac gaccggctcc
     1921 cgaaatccct gacttgctgt cgacgatcac ccccggctcg acgatgcctg ccttgaaggc
     1981 tggggcgagc gccaaaatcg ccgccgtcgg atagcagccg gggttggcaa cgaggcggct
     2041 cttgcgaatt gcctccctgt gcagctctgg cagcccgtag accgcaaatt tgagcgcctc
     2101 gggtgaagga tgttccaccc cgtaccattc gcggtactcg tccgggtcgc gcaggcgaaa
     2161 atcggcgctt acgtcgatca ccggcactcc gcgttccacg aatggcagca acgcggcagc
     2221 actggcagcg tgcggcaaac aggagatgac ggcatcaacg tcggtatcaa tctcaggcgt
     2281 gatcgtcaga tcgtagcgcc agagatgtgg aaaaacggcg gccagcggct tgcccgcttc
     2341 gctgcgtccg gtggccgcga ccatcgtggc gttcggatgc tgcaacaaga gacgggcggc
     2401 ttcgacgccc gcgtagccgg taacgttcag gacaccaacc cgaatcacac cacactccag
     2461 tgcataggta gcaagtataa agaagggctc acgcctgggc aacaaacagg aatgggacat
     2521 cgcggctcct tccaagggcc ggcaccgttg ttccctgcgt gaccatcggc gaaactgaga
     2581 tcagcttaaa cggttggaaa acggcgcggc gccgtcattc aaaacgaatg aggagtctgt
     2641 gttgtaccag gagctcagca ttttcaccgg caacgcccac ccggcgctgg ctcaggcgat
     2701 atgcgaccgt cttggccggc cccttggaca atgcgcggtc ttcgagttca gcaacgagaa
     2761 cattttcgtc cagattgaag agaacgtccg caaccgcgac gtcttcatca tccagccggc
     2821 cgcatcgccc gtgagcacgc gattgatgga actgttcatc atgctcgacg catttcggcg
     2881 cgcttccgcg ggacggatca gcgtcgtgat gccttactac gcctacggac gtagcgataa
     2941 aaaagaccag ccgcgggtcc cgataacggc ccgactgatt gcaaatatct tggaaactgc
     3001 cggcgcggac cgccttctga cagtggacct gcacgcggga cagattcagg gattcttcaa
     3061 tgtgcccgta gacgagttga gtacgctgca cagttttgaa atgtacttta gagagttgga
     3121 cctgccggac cccgtggtcg ttgcgacgga cctcggcgcc tccaagcggg cccgcaacct
     3181 ggcggaacgg ctggaggtcc cgctggccgt catggagaag cgacgcgctg ctaacgacga
     3241 tactgccgag atcatgacag tgatcggcga ggttaacggc aagaccgcgc tgatcgtcga
     3301 tgacgaagtc gatacggccg gtacgctcat ccaggccgtc tgtgcgctgc atgagcgcgg
     3361 cgccaaagcg atttacgccg ccgcaacgca cggcgtgctt tcagggccgg cgatcaagcg
     3421 aattgacgat agcccccttc aacaacttgt tgtgtcggac tcactcccaa ccccatcggg
     3481 caagcaatcg gataagatcc ggacgctgtc actggctcca atgctggccg acgcgatcac
     3541 gcgtatccat accggccagt cggtcggcgc catgttccat tagtagggaa acggctggta
     3601 gggaaacggt gaccggggcc ccctggtgta tcatttatcg acaataactc ttgctcctct
     3661 gcgttcggca aggtgattcc agatgaagtt tctcagcaaa ttgatcggcg atccgaatga
     3721 gaaagagatc aagcacctcc ttccgatcgt cgaagaggta aatgcgctcg agccggagac
     3781 acaagcgctc tccgacgagg agttgcgtgc caagaccgac gaattcaaac agcgacttgc
     3841 agatggcgag acgctggacg acctgttgcc ggaagctttc gccgtcgtgc gagaggcatc
     3901 acatcgctat acgggccgcc gtcattttga cgtccagatc atgggcggcg tcattctgca
     3961 tcgcggccag atcgccgaga tgaagaccgg tgaaggcaag acgctggtag cgacgctgcc
     4021 cctgtacctg aatgcgctta ccggcctcgg cgtacacctg gttacggtga acgattacct
     4081 ggccaagcgc gacccgcagt acatgggtcc aggcacgatc tttacccggc ttggtctttc
     4141 cgtaggcgtc ctgcagcacg aggccgcgtt tctggtctcg cacgacaagg taagcgatca
     4201 gccgaacgcg gaatacctcg tgccgtgcag ccgcaacgaa gcctacctcg ccgacgtgtt
     4261 gtacggaacc aaccacgagt tcggtttcga ctacctgcgc gacaacatgt cacgcagcct
     4321 cgacggctgc gttcagcgcg aactgtctta cgcgatcgtc gacgaggtcg acaatatcct
     4381 gatcgacgaa gcgcggacgc cgctgatcat cagcggaccg gcggaagaga acgtccagat
     4441 ttaccaggcg ttcgcgcgcg tggtgcccac gctcagtcta aataccgatt acgaagtcga
     4501 cgaaaagcag agggttgtga ccctgaccga cgagggcgtc gagaggatcc agcgcggact
     4561 tgggatcggc aacatctatt ccgccgagaa tttcaagttg acgcgcttcc tcgacgcggc
     4621 tctgaaagct catatcatct accagcgcga ccgcgactac gttgtcaaag atggcgaagt
     4681 gatcatcgtc gacgacttta ccggccggct catgcacggg cggcgttggt cagacggcct
     4741 gcaccaggcg gttgaggcta aagagcgggt caagattcag aacgagagcg taacgtacgc
     4801 aacgatcacg ctgcagaact actttcgcct ctacgagaaa ctcgccggta tgaccggtac
     4861 ggccatgacc gaggccgagg agttccacaa gatctataag ctcgaagtcg tttcggtgcc
     4921 aacaaaccag ccgatggtgc gcgttgacaa cggcgacctg gtttatcgaa ctgaagaggc
     4981 gaaatttgcg gctgtcgcga gcgaagtcat ggaacgcagc cagcgcggcc aacctgtact
     5041 cgtcggcacc gtttcaattg aaaagtctga ataccttgcc gagttgctgc gacgtcgcgg
     5101 cgtgaagctc gaagtcttaa acgccaagca acacgaacgc gaggcgtcga tcgtcgcgca
     5161 ggcgggacgc ctgggcggcg taacgatcgc cacgaacatg gctgggcgcg gtaccgacat
     5221 catcctcggc ggtaagccag acgagcgaga cgatgaagag tggaagaacg agcacaacaa
     5281 cgtagtcgag cagggcggct tgcacatcat cggcacagag agacacgagt cccggcgcat
     5341 cgacaatcag cttcgcggtc gagccgggcg gcagggcgac ccggggagct cgcgcttcta
     5401 cgtatcattc gaagacgatg tcatgcgccg gtttgcgccg gactggctgc cgggaatgat
     5461 gaccaagctc ggaatggatg acgagacgcc gatcgagcat tcatgggtca gtaaagcgat
     5521 ccatacggct caatcaaaag tagaaggcta caacttcgac atccggaagc acgtcgtgga
     5581 gtacgacgat gtgatgaaca tgcaccgcga cgtcatctac cgcgagcgac ggaagatcct
     5641 ggaaggtgcc gacgtgcgtg agaacgttcg caccatgtcc gaagaagagg tgggcgccgt
     5701 agtttcggca cacgtagacg accggccggc cgacgagtgg gacctggaga gcctgatggc
     5761 gcagatcaac gcgatcatgg aactgccgcc gggtctcgac caggcaacgc tgatcgaaat
     5821 gactcccgcg gacatcaaag agcagcttac tgaccacgcg cggcagctct acgacgcgaa
     5881 aatcgcccaa ttcgagcgag tgcgcgggat cgatccattc ggcgatgacc cgtttggctt
     5941 tgtcgaacgg caggtactat tgcaaaccat cgacgcgctc tggatcgagc acctaacggc
     6001 gatggaagag atgcgcgaag gcataggact gcaggcatac ggccagaacg acccgctcgt
     6061 catgtacaag cgcgaagcgc acgacatgtt cgagcaact
//
LOCUS       DATCPE010000067        14163 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U7219
            k141_530659, whole genome shotgun sequence.
ACCESSION   DATCPE010000067 DATCPE010000000
VERSION     DATCPE010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955033
            Sequence Read Archive: SRR1586279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 14163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 16:49:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 640
            CDSs (total)                      :: 631
            Genes (coding)                    :: 627
            CDSs (with protein)               :: 627
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14163
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_530659"
                     /isolate="SMAG_U7219"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1023)
                     /gene="recF"
                     /locus_tag="VIM45_02075"
     CDS             complement(<1..1023)
                     /gene="recF"
                     /locus_tag="VIM45_02075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HEY8820107.1"
                     /translation="MFLAHLTLNNFRNYHRLDLAATPGITLVAGANAQGKTNLLESVY
                     LLATTRPARGSSEAELVRWGSGGDGINATRVSGSAEREQGPVTVEVVIVGRDEATGPR
                     GRVEHASKRLKVNGIPRRASEVIGQITAVLFTASDIELLTGPPAWRRRYLDITIAQTD
                     SVYVRALQRYARIVLQRNHLLRRIDDGHARPEELAYWDAELVREGARIMHVRQGTVEA
                     VCIAAREYHRRLSDGREDLQVTYAPQTPIPEDDSPSIEAIEARLRVALGPVRKVEIAT
                     GNTLVGPHRDDLHFTLDGVPIGAYGSRAQQRTAALALRLAESSFLNAVNHDPPILLLD
                     DILSELD"
     gene            complement(1058..2041)
                     /locus_tag="VIM45_02080"
     CDS             complement(1058..2041)
                     /locus_tag="VIM45_02080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8820108.1"
                     /translation="MDQEYARPDLMVDQTDGAQEDDAEAFEHATAIVVDPLERTRAAL
                     EDARRQMPGMLGLETAADRPEIGEGQPDRFVGVRHLAAESTDLMTQLRDERSQLAEEI
                     TGLRLLVLDLATEVRRLAGMLDLVQHGTVRAADDENVPLALSSSAAVDPATTGLSELS
                     FDGSNVGNGSTLKMPPDFGWRDRPEASPGQPLDADGGPGPDATQPDDSTPGSDDDGVA
                     ADAGSDISAHREVPAGHAIRLLVIGVPGVRSVAAIEAGLATRFSPGHSELARFRGGDA
                     TFTLKPEADLSVDALVDAVEQGGLRVGECDVQDGGRVIRLLVAASPRAGTE"
     gene            complement(2339..4801)
                     /locus_tag="VIM45_02085"
     CDS             complement(2339..4801)
                     /locus_tag="VIM45_02085"
                     /inference="COORDINATES: protein motif:HMM:NF019935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEY8820109.1"
                     /translation="MNATIAGFLEWRESCIRTAPDDSGLALCHSLVAALDAALRSAFP
                     AHSGEFALVAIGGYGRRELCLYSDIDLMLLYRGWLPPGIVDTVFYPLWDAGLKVGHAV
                     RTVPEAVKSADERLETLTSLLDHRLVAGSPELVDRLSKDLAKLLKRRLPRVREELRTA
                     ERQRRNSERFQLLETDLKGGRGGLRTLQRERWLRRAEALAGVPQAAESCSDAVDAAHE
                     VLLRSRNALHAAAGRASDTWLFDLQEPACRWLGSDVDVWAPLLYGALRDIDHAAEEFW
                     EVTAPVKGEKSSGRLSGLVPRRRNALSVEREPPVAGQSVFAYAAGISPLDISPPRLPA
                     VAATAIGGAPGPSWTAEDRQGLVSLLRSGERGYRIFDALDRLGWIERALPEWRHVRGA
                     PQHVPFHENPVDTHLWRTVIEAIALAEGRTDDPWAAGIAIDLGGLDELLLASLLHDIG
                     KGWPGDHAETGAAAATAFCQRAHFGLEATLTVATSVRHHLLLPTIATRRDIADARVIE
                     DVADRAGRARTLRILYLLSVADSRATGPTVWNPWKASLIRSLFAAALEVLEMRAGASP
                     LSTSRAAARSMVLEAAEGRVAHAAVAAHIDGMAPGYLQSFGAADVIRHIELIDKTSKS
                     DAARLDVRGEGDAYDLVVTTPDRPGLIAMVSGVLALHNLSVLDGRFFTREDGVALQAL
                     HVVDALGNAVTEDRWRRVQTDLERALRGDLPLEQRLREKIHAYRGRRRRAQEAEVRFV
                     REASDEYTVVEVHAADRVGLLYAIAGTLFALCLDIHLAKIDTQGTSVVDVFYLRDLTG
                     APLRDAAKLEELRMGLLAAVES"
     gene            complement(4814..5152)
                     /locus_tag="VIM45_02090"
     CDS             complement(4814..5152)
                     /locus_tag="VIM45_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEY8820110.1"
                     /translation="MKLVIAFIKPFKLDEVKDALKAAGVTGLSVTDVRGFGRQAGHTE
                     VYRGAEYEVDFVPKSRIDVLVDDDMVATVVDTIERSAHTGKIGDGKIAVLPVVDLVRI
                     RTGERGHDAL"
     gene            complement(5228..6469)
                     /locus_tag="VIM45_02095"
     CDS             complement(5228..6469)
                     /locus_tag="VIM45_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018350994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEY8820111.1"
                     /translation="MDINSGDTAWVLASAALVLFMTPGLALFYGGMVRAKNVLGMLMA
                     NFFTITIVTIIWVLVGYTLAFAPDAWGGLIGKLDFIGLKDVRGNSLFPGTHIPDIVFM
                     CFQMMFAIITPALITGAIAERMKFSAWVVFCILWSLIVYLPVAHWAFASDGWIYAKLG
                     ARDFAGGLVVHINAGAAALACVFVLGPRRGFRREVIRPHNLTLTLLGAGILWFGWFGF
                     NAGSALGANRLAGSAFAATQIAAAVGAGAWIAMEWWRVGKPTTLGAASGAVAGLVAIT
                     PAAGFVSPLPAMAIGAVAGAVCFLAVGLKFRFNFDDSLDVVGVHLVGGILGSVLLGVF
                     AERAINPDQLSTNGLLFGGAAFFGKQVIAVLAVLAFSFVVSFILAKAVDMIMGLRVPD
                     EVETSGLDISLHEERAYVFAE"
     gene            complement(6674..7195)
                     /locus_tag="VIM45_02100"
     CDS             complement(6674..7195)
                     /locus_tag="VIM45_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8820112.1"
                     /translation="MMDDRTAVAATALRIDMQDAATEEEALAAIPGIVQVHRNPTGWY
                     NNASFALPLAAALVVAIAGLILRRGDVLAFAGFLFAVTACMVPVVLVSWRQTATMVVV
                     GADGITSLHGRHVLKSLPWTVVRSITRRETQGNVRWIVAADDDRLALDGELDHLDRLV
                     ATAQRLAGLSGVE"
     gene            complement(7192..8067)
                     /locus_tag="VIM45_02105"
     CDS             complement(7192..8067)
                     /locus_tag="VIM45_02105"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017954454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEY8820113.1"
                     /translation="MKVGVVFPQTEIGPDPAAVREYALASEAAGYDHLIVYDHVVGAD
                     PNRPGGWSGPYTHQSQFHEPFVLFGYLAAITRRLELVTGILISPQRQTVLIAKQAAAV
                     DVLTGGRLRLGLGVGWNAVEYEALGENFHNRGRRMEEQVDLLRKLWTQTVVTYNGKYH
                     HVTAAGINPLPIQRPIPIWMGGMSEPVLKRIARLADGWFPQTRPDDEGRVVIERLRGY
                     VQEAGREPSAVAIEGRLNAARSTADQWVDTAAKWRKLGATHISFNTMGAGFTSPQQHI
                     DTIRQFKEALAAARV"
     gene            8192..9421
                     /locus_tag="VIM45_02110"
     CDS             8192..9421
                     /locus_tag="VIM45_02110"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002620748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY8820114.1"
                     /translation="MTRTALNGLSRREDATMFKQRLNFGIFMAPFHHVGDNPTLALQR
                     DLELIEWLDHLGFDEVWIGEHHSAGWELIASPELMIAAAAERTKRIKLGTGVTSLPYH
                     HPLIVADRMVQLDHMTRGRAMLGVGPGGLTSDAYMMGIEPATQRQRMDEALGAIIELL
                     KGDEPVTMKTDWFELREARLQLLPFTKPCFPIAVSSTISPSGMMTAGKYGVGVLSIGV
                     SIAGGTDGLRRQWGMAEEAAQAAGTVIRREEWRLLKSFHIAETREQAFRDVEAGERRE
                     TVNYFQGTLGQPAGPQSLDELVKMGVAVVGTPDDAIEAIENLGELSGGFGGFLGRAHE
                     WANREATMRSFELFARYVMPRFQGVLPAVKASNEWVSDNRRTIFSPSQAAIVKAFEDA
                     GKEMPKEIKERLLRTRP"
     gene            complement(9446..11344)
                     /gene="speA"
                     /locus_tag="VIM45_02115"
     CDS             complement(9446..11344)
                     /gene="speA"
                     /locus_tag="VIM45_02115"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011144058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HEY8820115.1"
                     /translation="MGEWTTQASADLYRLQGWGEPYFSINDAGHLEVSPAGDGGPRID
                     IYRLVRDLQTRNLSLPLLLRFSDILGDRIRRLNESFTHAIAEYEYPGVYRGVYPVKVN
                     QQRHLLEEIVALGGPYHLGLEAGSKPELLIAVATLDTQDALIVCNGFKDEEYIETALL
                     ARQLGKRPFIVIERMEELDMVLKATKKLGIEPILGVRAKLTARGNGRWGISTGDGSKF
                     GLTASEIVEVAERLRSAGLLHTLQLLHFHIGSQISQIGVIKSALREASQFYVELAGMG
                     APMGFLDVGGGLGIDYDGSKTDFYASKNYNVQEYAYDVIATVQSACDRRDITPPTLVS
                     ESGRAMVSHQSVLVFDILGVRKVESGTPEPPDAGDHQLVHELWETYRTVDAENLQEAY
                     HDAVAAKDETQTLFSLGYMGLEQRAKAERLFWACCACILQHLRGAEHVPEELEDLEEL
                     LADTYFGNFSVFQSIPDSWAIDQLFPIMPVHRLGEEPRRRATLADLTCDSDGRIDQFI
                     DLQDVKHVLELHEPDGEPYYLGIFLAGAYQEILGDLHNLFGDTNAVHVHATEGGYRLE
                     HVIKGDTVSEVLRYVQYDGEILLEKVRRQAERALQNRQLTLEQSRLLLQHYEDSLRGT
                     TYLRGQHA"
     gene            complement(11455..11952)
                     /locus_tag="VIM45_02120"
     CDS             complement(11455..11952)
                     /locus_tag="VIM45_02120"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY8820116.1"
                     /translation="MVNTSHPAVEAYRRLYVAHTAVARAIERDLIRVGVSLPQAIALQ
                     VIGTAADAVTATQLAGYMGQERQSMTGLIDRMEMKLWIARVRDLPDRRAIRLQLTPSG
                     RVKLDEILPITFSAVVSIFSRFTEGDLIGMARTFEMVYDAAATQPGMDLPAVFEPETA
                     SVALA"
     gene            12274..12780
                     /locus_tag="VIM45_02125"
     CDS             12274..12780
                     /locus_tag="VIM45_02125"
                     /inference="COORDINATES: protein motif:HMM:NF013411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl tetrahydropterin synthase family
                     protein"
                     /protein_id="HEY8820117.1"
                     /translation="MPAHQNPSRSAHVERQRLKFAAAHMATFGGSIEPLHGHTYAVTI
                     EVDGELSSEGWVVDFGVLKRIGVEICDRLDHHFLLQRGSALLGMERGDGAWEISFADK
                     RYRLPEDDVFELPITNTTAEQIAEWFWGEVLGNLRTAGITTVTRLTIGVEEAPGQAGW
                     FAADLPVD"
     gene            12793..13590
                     /locus_tag="VIM45_02130"
     CDS             12793..13590
                     /locus_tag="VIM45_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019875386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEY8820118.1"
                     /translation="MAVTYEKNGHKAYITLNRPEALNAFNTDMGRELREAWLDFRADP
                     NVWVAIVTGNGRAFSSGADVKELARANSGAASGGQTVNYSGFWSSMYGLDSFEAGLEV
                     WKPTIAAVNGHCLGIGCTLAITCDFRLASDRAQFGFPEVKIGVPTIMASIRLPKLVGM
                     GPALELLLYGDSIDAQEAYRVGLVHRVIPHERLLAAADEWADRILLRGPTAVAATKEV
                     TVRCSNLPLPDAVRMGEAIRRLARTADDAKEGPRAFAEKRTPIWTGR"
     gene            complement(13601..13807)
                     /locus_tag="VIM45_02135"
     CDS             complement(13601..13807)
                     /locus_tag="VIM45_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8820119.1"
                     /translation="MLILKACPKCKGDLAAETSVRRMSDDSDIICIQCGYTLPLEQQK
                     SLFARLLSRTQPAVLERVPEYART"
BASE COUNT         2699 a         4538 c         4331 g         2595 t
ORIGIN      
        1 gtcgagctcc gagaggatat cgtccagcaa cagaatcggg ggatcgtggt tgacggcgtt
       61 cagaaatgac gactcggcca ggcgcaacgc cagcgccgcg gtccgctgct gagcccgcga
      121 gccgtatgcg ccgattggca ccccatcgag ggtgaagtga aggtcgtcgc gatgaggccc
      181 aaccagcgtg tttccggtgg cgatctcaac ctttcgcacc gggcccagcg cgacccgcaa
      241 tcgcgcttca attgcctcaa tcgacgggga atcgtcttcg ggaatcggag tctgcggggc
      301 gtaggtgacc tgaagatcct cacgtccgtc actcagccgc cggtggtact cgcgggcggc
      361 gatgcagact gcctcaaccg tcccctgtcg aacgtgcatg atacgagcgc cctctcgcac
      421 cagttcagca tcccagtatg ccagttcctc aggcctcgca tggccgtcgt cgatgcgacg
      481 caagagatgg ttgcgttgca aaacgatccg cgcgtaccgc tgcaaagcgc gcacatacac
      541 gctgtcggtc tgcgcgatgg taatgtcgag ataccggcgt ctccaggcag gtggacccgt
      601 gagcagctcg atgtctgatg cggtaaacaa gaccgcggta atctgcccaa tcacttcgga
      661 ggcgcgccgc ggaatgccgt ttaccttaag ccgcttgctc gcgtgctcga ccctgccgcg
      721 aggccccgtc gcctcatcgc gaccgacgat gacaacttcc accgtcaccg gaccctgttc
      781 ccgctctgca ctgccggaca cccgcgtggc gttgatgccg tccccaccgg agccccaccg
      841 caccaattcg gcctcgctgc tgccgcgcgc gggcctggtt gtagctagca ggtacaccga
      901 ttcgagcagg ttggtcttgc cctgggcgtt cgctccggcg acaagcgtga tgccgggagt
      961 cgcagcaagg tccagccgat ggtaattgcg aaagttgttc agggtcaggt gcgctaagaa
     1021 cataccgccg cccgcgctgc cgccggccgc gcgcggctca ttctgtgccg gcccgcggag
     1081 aggccgcgac gagcagccgg ataacacgcc ctccgtcctg aacgtcgcat tcgccgacgc
     1141 gcagcccgcc ttgctcgacg gcgtccacca gcgcatcgac tgacaggtca gcctccggct
     1201 tcaacgtgaa ggttgcatcg ccgccccgaa agcgggcgag ctcactgtgt cccggagaaa
     1261 aacgcgtcgc caggccggct tcgatcgcag caacagaacg aacgcccggg acaccgatga
     1321 ccaggagcct tatcgcatgg ccggctggaa cttcgcggtg cgcgcttatg tccgatccag
     1381 cgtccgcggc tacaccatcg tcatcggacc caggtgttga atcatccggc tgcgttgcgt
     1441 caggtcccgg acccccgtcc gcatccaacg gctgtcccgg gctcgcttcc ggacggtctc
     1501 tccacccgaa gtcaggcggc atcttcaagg tcgagccgtt cccgacgttg gagccatcga
     1561 agcttaactc cgacaacccc gtcgtcgccg gatcgacggc agcactgctg ctgagagcga
     1621 gcggcacgtt ttcatcgtcc gccgccctga ccgtaccgtg ttgcaccaag tcaagcatgc
     1681 cggcgagccg gcgcacttcc gtggccaaat caaggacgag caaccgcaga ccggtaatct
     1741 cttccgcaag ctggctgcgc tcgtcgcgca gctgggtcat cagatcggtc gactcagccg
     1801 ccaggtgcct gacgccaacg aaccggtcag gctgcccttc cccgatctcc ggccgatcgg
     1861 ccgcggtctc caggccgagc atccccggca tctggcgtcg cgcatcttca agtgccgcgc
     1921 gggttcgctc cagcggatcc accactatcg cagtcgcatg ctcaaacgcc tctgcatcgt
     1981 cttcttgcgc gccgtccgtc tggtccacca tcaaatccgg ccgggcatat tcttggtcca
     2041 ccgacggaat ccctccctcc gctaccgcag cctgcaagga tgagtcggct cctgtcgggt
     2101 cggcaagggt aacgttgtag atcgcagacc attcaagctc ggcaggcggt tctacaagga
     2161 tccggtgacg ccgcggtgac catgggttcg cgaatgcctc cacgcggcga agtgctcgcc
     2221 cgagcgctcg acgctttctg cgtggctcgt accagtcgct cacagtcgct cctggatgcg
     2281 gacaagccgc cccgagatgg ttagatgtca gtataccgtg cgccggccaa tgtacgggtt
     2341 agctttctac cgccgcgagc aatcccatcc gcagctcttc gagctttgcg gcatcccgga
     2401 gcggagcgcc ggtgagatcg cgcaggtaga agacgtctac gacgctggtt ccctgtgtgt
     2461 cgattttagc gaggtggatg tcgaggcaga gcgcgaacag cgtcccggca atcgcgtaca
     2521 acagtcctac gcggtccgcg gcgtgaactt cgaccacggt gtattcgtcc gacgcctcac
     2581 gcacaaaccg gacctcggcc tcctgcgcgc gccgtcgacg gccgcggtac gcatgaattt
     2641 tttcccgcag gcgctgctcc agcggcagat cgccgcgtag tgctcgctcg aggtccgtct
     2701 gcacccgtcg ccagcgatcc tcagtgacag cgtttcccag ggcatcgacg acatgcagcg
     2761 cctgcagcgc gacgccgtcc tcgcgggtaa agaagcggcc atcaaggacg ctcaggttgt
     2821 gaagcgccag gacgccggaa accatcgcga tgagacccgg tctatccggc gtggtcacga
     2881 cgaggtcgta cgcatcgcct tccccgcgca cgtcgagccg tgcggcatcg gacttgctcg
     2941 ttttatcgat caattcgatg tggcggatga cgtctgccgc accgaatgac tggagatagc
     3001 cgggagccat tccgtcgata tgggcggcca cggcggcatg cgcgacacgg ccttcggcgg
     3061 cctcgagcac catggaccgc gcagccgccc gcgaagtgga aagcggggag gcgcccgctc
     3121 gcatctccaa gacctcgagc gccgcggcga acagtgaacg aatcaggcta gctttccagg
     3181 ggttccatac ggtcgggccg gtagcacgcg agtccgctac ggacagcaga tacaagatgc
     3241 gcagcgtgcg cgcccgaccc gcccggtcgg cgacgtcttc aatcacgcgc gcgtccgcga
     3301 tatcgcgtcg agtcgcaatc gttggcagca gcaggtgatg cctgacgctt gtcgcgacgg
     3361 taagcgttgc ctccaggcca aagtgtgccc gttggcagaa tgccgtcgct gcagcggcgc
     3421 cggtttcggc gtgatctccc ggccagcctt tgccgatgtc atgcaggagt gatgccagca
     3481 gcaactcgtc gaggccgcca aggtcgatgg cgatgccggc tgcccatgga tcgtcggttc
     3541 taccttcggc cagcgcgatc gcttcgatca cggttcgcca gaggtgcgtg tcgacgggat
     3601 tctcgtgaaa cggcacgtgt tgcggcgccc ctcgaacgtg ccgccattcg ggcaatgcgc
     3661 gttcgatcca tcccagacga tccaatgcgt cgaatatccg gtagccgcgt tcgccggatc
     3721 tcaacagcga caccagcccc tggcggtctt cggccgtcca cgacggcccc ggcgctccgc
     3781 cgatagccgt tgctgccacc gcgggcaacc gtggcgggct tatgtcgagc gggctgatgc
     3841 cggcggcgta ggcgaagacc gactgtccag cgaccggcgg ttcccgttcg acactcagcg
     3901 cgttgcggcg tcgtggtacc agtccgctca gtcgtccgct actcttttcg ccctttactg
     3961 gggccgtcac ttcccaaaat tcctcagccg cgtgatcgat gtctcgcaag gcaccgtaga
     4021 gcagcggtgc ccaaacgtcg acgtcggatc ccagccatcg gcaggcaggc tcctgcaggt
     4081 cgaacagcca ggtatcgctg gcgcgtccgg ccgccgcatg cagggcgttg cgagagcgca
     4141 gcaacacttc gtgcgctgca tcgactgcgt cactgcagga ctccgccgcc tgcgggacac
     4201 cggcaagcgc ttcggcccgc cgcagccagc gttcccgctg tagtgttcgc agccctcccc
     4261 ggcccccctt gaggtccgtc tccaatagtt ggaaccgttc gctgttccgg cgctgccgct
     4321 ccgccgtcct caactcttct cgtactcgcg ggagcctgcg cttcagcagc ttcgcgaggt
     4381 ctttggacaa gcggtcgact agctcgggcg aacccgcgac aagacggtgg tcgagcagcg
     4441 aggtcaacgt ttccagccgc tcgtccgcag acttaaccgc ttctggcacc gtgcgcactg
     4501 cgtgaccaac cttcagcccg gcgtcccaca gtggatagaa aacggtgtcg acaatcccgg
     4561 gcggcaacca accacgatac agcagcatca ggtcgatatc ggagtacagg cacagctcac
     4621 gccgcccgta gccgccaatc gcgacgagcg caaattcgcc gctgtgcgcg ggaaatgcgg
     4681 aacgcagcgc ggcatcgagc gcggcgacca ggctgtggca gagtgcaagc cccgagtcat
     4741 caggggcggt ccgaatgcag gactcgcgcc attcgagaaa accggcgatt gtggcgttca
     4801 cggtcaccgc gtgttacaga gcgtcgtgac cgcgctcccc ggtacggatc cgaaccaggt
     4861 ccacaaccgg aagaacggcg atcttgccat cgccaatctt acccgtatgc gcgctccgtt
     4921 caattgtgtc gacgaccgtc gccaccatat cgtcgtccac gagcacatcg atgcgacttt
     4981 tggggacgaa gtccacttcg tactcggctc cccggtagac ctcggtatgc cccgcctgac
     5041 ggccgaaacc gcggacgtcg gtcacactta gtccagtaac cccggccgcc ttcagggcgt
     5101 ctttgacctc gtccaacttg aaaggcttga tgaaggcgat gacgagcttc atctgctgct
     5161 ctcctcagcg cgccaacggc gtgctttgtt gctgtcgatt cggcagccgc ctgggaggcg
     5221 ccggacccta ttcggcgaac acgtaggcgc gctcttcgtg cagagagatg tcgagaccgc
     5281 tcgtctcgac ctcatccggg acacgcagcc ccatgatcat atcgacggct tttgcgagga
     5341 tgaagctgac gacgaagctg aaagcgagca cggctagaac agcaatcact tgcttaccga
     5401 agaaagcagc cccgccgaac aggaggccgt tcgtgctcaa ctgatcgggg ttaatcgccc
     5461 gctccgcgaa cacgccgagc agcaccgaac caaggatacc gcccacgagg tgaacgccaa
     5521 ccacgtcgag cgagtcgtcg aaattgaagc gaaacttgag tcccacggcc aaaaagcaca
     5581 ctgctccggc gaccgcgccg atcgccatcg ccggtaaggg gctgacgaag ccggccgccg
     5641 gagtaatcgc aaccaacccc gccaccgcac ctgatgcggc gccaagcgtt gtcggcttcc
     5701 ccacgcgcca ccattccatc gcaatccagg ccccagctcc gaccgccgcg gcgatctggg
     5761 tggccgcgaa ggcgctgccc gcgagccggt tcgcgccgag cgctgaaccg gcgttaaagc
     5821 cgaaccaccc gaaccacagg atgccggcgc ccagcagcgt caacgtgagg ttgtgcggcc
     5881 ggattacttc gcgacgaaag ccgcggcgtg gacccagcac gaagacgcac gccagcgcag
     5941 cggcaccggc gttgatgtgg acgaccaacc cgccggcaaa gtcccgcgcg cccagcttag
     6001 cgtagatcca gccgtccgaa gcgaacgccc agtgagcgac cggcaggtac acaatcagcg
     6061 accagagaat gcaaaatacg acccatgccg aaaacttcat ccgttccgct atggctccgg
     6121 ttatgagcgc gggtgtgatg atcgcaaaca tcatctggaa gcacatgaag acgatgtccg
     6181 ggatgtgtgt tccgggaaac aggctattgc cgcgcacgtc tttgaggccg atgaagtcca
     6241 gctttccgat cagccctccc caggcatccg gcgcgaacgc gagcgtgtat ccgaccaaca
     6301 cccagatgat cgtgacgatc gtgatcgtga agaaattcgc catcagcatg ccgagcacgt
     6361 ttttcgcgcg gaccatgccg ccgtagaaaa gcgccagccc gggcgtcata aacaggacga
     6421 gagcggcgct ggcgagcacc cacgcggtat cgccggagtt gatgtccatt gactcgcggc
     6481 ctccgcgacg cgtgccgtca ctcgacacgg tccacgcacg ccttagcatg ctacagcgac
     6541 atttcgggga ggttgcgtat acgtttcttt accgttacag cacctgtgaa cagggacgca
     6601 cggcgtaacg caatgcaacc atggcgctca ctaaccggcg gcgacggcta cccgagttac
     6661 cgtgagggcg agatcattcg acgccggaca acccggccag ccgttgcgcg gtggcgacca
     6721 gccgatcgag atggtcgagt tcaccgtcca aggccagacg gtcatcgtcc gccgcaacga
     6781 tccaacggac gttcccctgg gtttcgcgcc gagtaatcga gcgcactacc gtccacggca
     6841 gcgacttcaa tacgtgccgg ccgtgcaagc tcgtgattcc gtcggcgccg actacgacca
     6901 ttgtcgccgt ctgccgccat gacacgagta ccacgggtac catgcacgcg gttacggcga
     6961 ataaaaagcc ggcgaaagcg agcacgtctc cgcggcgcag gatgagtccg gcgatcgcca
     7021 ccaccagcgc cgcggccagc ggtaaggcaa agctggcgtt gttgtaccag ccggtcggat
     7081 tgcggtgaac ctgcacgatg ccgggaatcg cagcgagcgc ctcctcttca gtggccgcgt
     7141 cctgcatgtc aatcctcaat gccgtagctg ccaccgccgt gcggtcatcc atcaaaccct
     7201 ggctgcagcg agcgcttctt tgaactgcct gatggtgtca atgtgctgct gcggcgacgt
     7261 gaagcccgcc cccatcgtgt tgaacgaaat atgggtagcg ccgagtttgc gccatttagc
     7321 cgccgtgtcc acccactggt cggcggttga cctcgcggcg ttgagcctgc cttcgatggc
     7381 gacggctgag ggttcgcggc cagcctcctg aacgtagcct cggagccgtt cgatcacaac
     7441 acggccctcg tcgtcggggc gcgtctgcgg aaaccagccg tcagcaaggc gagcgatgcg
     7501 cttcaacacc ggttcggaca tgccgcccat ccagatcggg atcggccgct gtatcggcag
     7561 cggattgatg ccggccgcgg tgacgtggtg gtacttgccg ttgtacgtga cgactgtttg
     7621 cgtccacaac ttgcgcaaga gatcgacctg ctcctccatg cgccgcccgc ggttgtgaaa
     7681 attctcgcca agcgcctcgt attcgacggc attccacccc acgccgagcc cgagccgcag
     7741 ccgccctccc gtcaggacgt ccaccgccgc cgcctgtttg gcgatcagca ccgtctggcg
     7801 ctgtggagat atgaggatcc ctgtgacgag ctccagccgc cgcgtaatcg cggcaaggta
     7861 gccgaagagg acaaacggtt cgtgaaactg gctttgatgg gtatacggtc cgctccagcc
     7921 gccaggacga tttggatcag caccaactac gtggtcatac acgatcaggt gatcgtaccc
     7981 cgccgcctct gaggccagcg catactcacg gaccgccgcc gggtccggac caatctcggt
     8041 ttgaggaaag acaactccga ccttcacgat tcattctcca ctggcgccca gaaggctatt
     8101 ttgcacagca tacgccgttt ccgaccagtc gatcgcgtaa tggtgtgtgg tgtccggagg
     8161 tgctggtcgc tcatagtagt atccgagctg agtgacgcga accgcgctca atggattgtc
     8221 gcgacgagag gacgcaacga tgttcaaaca acgattaaac ttcggcatct tcatggcgcc
     8281 attccaccac gtcggtgata atccgacgct ggcgctgcag cgcgatctcg agttgatcga
     8341 atggctcgac cacctgggat tcgacgaagt gtggatcggc gagcaccaca gcgcgggctg
     8401 ggagctcata gcctcgccgg agctgatgat cgccgccgcg gcggaacgca ccaagcggat
     8461 taagctgggg accggtgtca ccagcctccc ctaccaccac cccctcatcg tcgcggaccg
     8521 catggttcag ctcgaccaca tgacccgcgg ccgcgcgatg cttggcgtcg gtcccggcgg
     8581 gctcacctcg gacgcttaca tgatgggcat cgagcccgcc acccaacgcc agcgcatgga
     8641 cgaggcgctg ggtgcgatca tcgaactgct gaagggagac gagccggtca cgatgaagac
     8701 tgactggttc gagctgcgtg aggcgcgcct gcagttgctc cccttcacga aaccatgttt
     8761 cccgatcgcg gtctccagta cgatatcgcc gtcaggaatg atgacggccg gcaagtatgg
     8821 ggtgggcgtg ctgtcgatcg gggtcagcat agccggcggc accgacgggc tgcgcaggca
     8881 atggggaatg gcggaagagg cggcgcaggc ggcgggtacg gttattcggc gtgaagagtg
     8941 gcgtctgctg aagtcgttcc atatcgccga aacacgcgag caagcgttca gggacgtgga
     9001 agccggggag cgccgggaga cggtcaatta cttccagggc accctcggcc agcctgcagg
     9061 gccccaatct ctcgacgagt tggtcaagat gggggtcgcg gtcgtcggaa ctccggatga
     9121 cgccattgaa gcgattgaaa acctgggaga gctgagcggc ggttttggcg gtttcctggg
     9181 gagagcgcat gaatgggcaa accgcgaggc gacgatgcgc agctttgagc tgttcgcgcg
     9241 ctacgtcatg cccaggttcc agggagtcct tcccgcagtc aaggcttcca atgaatgggt
     9301 ctccgacaat cgacgaacca tctttagccc cagccaggcg gcgatcgtca aagccttcga
     9361 ggatgccggc aaggagatgc cgaaagagat caaggagcgc ttactccgca cgcgaccctg
     9421 accgagctcg cgtcgcccca ccccctcatg catgctgccc gcgcaggtag gtcgtgccgc
     9481 gcaaactgtc ttcgtagtgc tgcagcagca gccgcgactg ttcgagcgtt aactgcctgt
     9541 tctgcagagc ccgctcggcc tggcggcgca ctttttccag caatatctca ccgtcgtact
     9601 gcacgtagcg cagcacctcc gacaccgtgt cccctttgat aacgtgttcg aggcgataac
     9661 cgccttccgt cgcatggacg tggacggcgt tggtgtcccc aaaaaggttg tgcagatccc
     9721 cgaggatctc ctggtaggcg cccgcaagga agatacccag gtagtacggc tccccatccg
     9781 gttcgtgaag ctcgagcacg tgctttacgt cctgcaggtc gatgaactgg tcgatgcggc
     9841 catcgctgtc gcacgtcagg tccgcgagcg tggcgcgccg cctcggctct tcgccaagcc
     9901 tgtgcaccgg catgattggg aaaagctggt ctattgccca gctgtcgggg atcgactgga
     9961 ataccgagaa gttaccgaag tatgtatcag cgagcaactc ctcgagatct tccaactcct
    10021 ccggaacgtg ttctgccccg cgcaggtgct gcagaatgca agcgcagcac gcccagaaga
    10081 ggcgttcagc cttggctcgc tgttcgagtc ccatgtaccc cagcgagaag agcgtctggg
    10141 tctcgtcctt cgcggcaacg gcgtcgtgat acgcctcctg gaggttctcg gcgtccacgg
    10201 tgcggtacgt ctcccagagt tcgtggacca actggtgatc gcccgcatcc ggcggctccg
    10261 gcgtgccgct ttccaccttg cggacaccca ggatgtcgaa gaccagcacc gactgatgcg
    10321 acaccatcgc gcggccgctc tcactgacta gcgtgggagg cgtgatgtcg cggcgatcgc
    10381 aggccgactg gacggtcgcg atcacatcgt aggcgtactc ctgaacgttg tagttcttgg
    10441 acgcgtagaa gtcggtcttc gagccgtcgt aatcgatgcc aagaccgccc ccgacgtcca
    10501 ggaatcccat cggcgcgccc atgcccgcaa gctcaacgta gaactggctt gcttcacgta
    10561 gcgcggactt gataacgccg atctgcgaga tctgcgaccc gatatggaag tgcagcagtt
    10621 gcaaggtgtg gagaaggccg gccgaccgta gtcgttccgc tacttcaacg atttccgagg
    10681 cggtgagccc gaacttggaa ccgtcaccgg ttgaaatccc ccagcgaccg ttgccgcgag
    10741 cagtcaactt ggcgcgaaca ccgagaatcg gctcaatgcc aagctttttc gttgctttga
    10801 gcaccatatc gagctcttcc atgcgctcga tcacgatgaa cggtctttta ccgagctggc
    10861 gcgccagcaa cgccgtttcg atgtactcct cgtccttgaa gccgttgcag acgatcaacg
    10921 catcctgggt atccagcgtt gccacggcga tcagcagttc cggcttggaa ccagcttcga
    10981 gaccgaggtg atacggtccg cccagcgcaa cgatttcctc gaggaggtga cgctgctgat
    11041 tgaccttcac gggatacaca ccgcggtaga cgcccggata ctcgtactcg gcgatggcgt
    11101 gtgtaaagga ctcgttcaag cgccgaattc ggtcgccgag gatatcggaa aagcggagca
    11161 atagcggcaa tgataagttg cgggtttgga ggtcgcgaac gaggcgataa atgtcgatgc
    11221 gcggaccgcc gtcgccagcg ggcgagacct cgaggtgccc ggcgtcgttg atgctgaagt
    11281 acggctctcc ccagccctgc agacgataaa gatcagcgct ggcctgggtg gtccattctc
    11341 ccacggagac gttcctccag tttgcagtct ggcagccgtc gcccgtgtcg gggttacctt
    11401 ggccgtgcta cggaaaggat attgtccgtg acgtggcgat cccggccggc gcagctacgc
    11461 cagcgctacc gacgcggttt ccggctcgaa aaccgcggga aggtccatgc ccggctgcgt
    11521 ggcggcagcg tcgtagacca tctcgaacgt acgcgccatg ccaatcaggt cgccctctgt
    11581 aaatctcgag aaaattgaca caacggcgct gaatgtaatc ggcaggatct catccagttt
    11641 gacccgtccc gaaggcgtga gttgcagccg aatcgcccgg cgatcgggca ggtctcgcac
    11701 gcgggcaatc cacagcttca tttccatccg gtcgatcagg ccggtcatac tttgccgttc
    11761 ctgacccatg tagccagcaa gctgggttgc ggtaaccgca tcggccgcgg tgccgatgac
    11821 ctggagggcg atggcctggg gaaggctcac accaacgcga ataaggtcgc gctcaatcgc
    11881 gcgggcaacc gcggtatgag ccacgtatag gcggcgatag gcctcgacag ccggatgcga
    11941 cgtgttaacc atgctcccac ctgccttact aatgtcggcc gttatgttag gccgccgttg
    12001 cgttttccgc agttcagccc gatacctcat cgggcagggc tgaaatgtgt tgcgcgcctt
    12061 acttgccgac catcgtatca ccaatcacga tggaatgcta cacatttctt tcagattctg
    12121 tacttgaaca gttgggacat agacagacct tctaaggcga atgtgcatcg ccaacacttt
    12181 ggacatcggt tcctgaatcg caacatagct tgctgccggg gataaggaac cttagggtga
    12241 aactgccgcc gcatgccgcg gcccggagga gagatgcccg cccaccagaa tccgagtcgc
    12301 tccgctcacg tcgaacggca acgactcaag ttcgccgcgg cacacatggc gacgttcggg
    12361 ggcagcatcg aaccgctgca cggacacacc tacgctgtca cgatcgaggt cgatggagag
    12421 ctttcgtcgg aaggctgggt tgtcgatttc ggcgtcctga agcgaatagg cgtggaaatc
    12481 tgcgaccgcc tcgaccatca cttcctttta cagcgtggga gcgctctact tggaatggaa
    12541 aggggagacg gcgcgtggga aatctcattc gccgataagc gctaccgctt gccggaggac
    12601 gacgtattcg agctgccgat tacaaacacc acggccgaac agatcgcgga gtggttctgg
    12661 ggagaagtat tgggcaattt gcgcaccgcg gggattacaa cggtaacgag actgacgatc
    12721 ggcgtggaag aggcgccggg gcaagcgggc tggttcgcgg cagatcttcc cgtcgactga
    12781 aaggagcgat acatggccgt cacatatgaa aagaatggcc acaaggcgta catcacgcta
    12841 aacaggccgg aggcgctcaa cgctttcaac accgacatgg gccgcgagtt gcgtgaggca
    12901 tggctggact tccgtgcaga tccgaacgtt tgggtggcaa tcgtcacggg caacggtcgt
    12961 gcgttttcat cgggggctga cgtcaaggaa cttgcacggg ctaactcggg cgcggcgtcc
    13021 ggcggacaga cagtcaacta cagcggtttt tggtcttcga tgtacgggtt ggacagcttc
    13081 gaggctggcc tggaggtttg gaagccgaca atcgcggcgg tcaacggcca ctgcctaggg
    13141 atcggctgca cgcttgcgat cacgtgtgac ttcaggctgg cgtcggaccg ggcgcaattt
    13201 ggcttcccgg aagtcaagat cggcgtccca acgatcatgg cgtcgatccg gctccccaag
    13261 cttgtcggca tgggaccagc gctagagtta ctgctttacg gcgactccat tgacgcgcag
    13321 gaggcgtatc gcgtcggcct cgtccatcgg gtaattccgc atgagcggct actggcggca
    13381 gcggatgagt gggccgaccg catcctgctg cgcggtccca ctgccgtggc agccaccaaa
    13441 gaagttacgg ttcgctgctc aaacttgccg ttgccagacg cggtgcggat gggggaggcg
    13501 attcgccggc tcgcgcgcac ggctgacgat gctaaagagg gtccgcgtgc ttttgccgag
    13561 aagcggaccc cgatttggac cggccgctga tcacaggcgg ctacgtccgt gcgtactccg
    13621 gcacgcgctc cagcacagcc ggctgggttc tggacagcag ccgcgcgaac aggctcttct
    13681 gctgctccaa cggcaacgta tagccgcact gtatacagat gatgtcggag tcgtcgctca
    13741 tccgacgcac gctcgtctct gccgcgagat cgcctttgca cttcggacaa gccttgagaa
    13801 tcaacatgtg aactgctcca ctagtccggc gggcaggctc ccttttcaac ctgagttcac
    13861 ggtacgccga taccggtgac ggcagtcatg ccccgccgcg ctatccccgg ctctgtcgta
    13921 ctaacctgtt tgtggtagcg tgagccggtt ttactcgcta aagcgtctca gttgctaaat
    13981 tcatacgtag gagttttgca cgttacggcg tcaaacacga gtggtcgaaa ttgcggggtg
    14041 ttgacatccg tgttatactc cggccacttc gctcgcgccc gaatttcagc aaaggttgcg
    14101 gcgtggttca ggcagcggac tgtggatgtt ccctcgggcg gtttcgcgaa ggtgctgtcc
    14161 cat
//
LOCUS       DATCPE010000068         5330 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U7219
            k141_303509, whole genome shotgun sequence.
ACCESSION   DATCPE010000068 DATCPE010000000
VERSION     DATCPE010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955033
            Sequence Read Archive: SRR1586279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 5330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 16:49:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 640
            CDSs (total)                      :: 631
            Genes (coding)                    :: 627
            CDSs (with protein)               :: 627
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5330
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303509"
                     /isolate="SMAG_U7219"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            44..117
                     /locus_tag="VIM45_02140"
     tRNA            44..117
                     /locus_tag="VIM45_02140"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:78..80,aa:Val,seq:cac)
     gene            153..240
                     /locus_tag="VIM45_02145"
     tRNA            153..240
                     /locus_tag="VIM45_02145"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:187..189,aa:Leu,seq:cag)
     gene            259..335
                     /locus_tag="VIM45_02150"
     tRNA            259..335
                     /locus_tag="VIM45_02150"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:293..295,aa:Arg,seq:gcg)
     gene            421..789
                     /locus_tag="VIM45_02155"
     CDS             421..789
                     /locus_tag="VIM45_02155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8820120.1"
                     /translation="MIPEGTKPNEDERYLSLGAVAGRFLVRIDDVHRWIAQGRLKTVT
                     GPRGKTLIPESALLDFQRTAYRRENYNGTDHGLHSWPTDIPEVSNLPMIPVQNFQQAE
                     ANAPIWPFPHAKPRMAAKGD"
     gene            780..853
                     /locus_tag="VIM45_02160"
     tRNA            780..853
                     /locus_tag="VIM45_02160"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:814..816,aa:Val,seq:tac)
     gene            949..2595
                     /locus_tag="VIM45_02165"
     CDS             949..2595
                     /locus_tag="VIM45_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011734468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEY8820121.1"
                     /translation="MSLHEWREATYARALKRGAERQPEFETTGAMSIAPLYTPESLNG
                     WNYQEQLGYPGEFPYTRGVQPTMYRGRFWTMRQYAGFADAEESNRRYRYLLEQGQTGL
                     SVAFDLPTQIGYDSDDEMAQGEVGKVGVAIDSLLDMEILFNQIPLEQVTTSMTINATA
                     PILLALYVAVARKQGADLTKLGGTIQNDILKEYVARGTYIFPPKPSMRLITDVFAWCK
                     DNLPAWNTISISGYHMREAGSTAAQEIAFTLADAIAYVEAAQNIGLKFDEFGPRLSFF
                     FACHNNFLEEVAKFRAARRLWAKIARGRFGSTDPRSQMLRFHTQTGGATLTAQQPDNN
                     IVRTTIQALAAVLGGTQSLHTNSKDEALALPSEHAVQIALRTQQVIAYESGSGDTVDP
                     LAGSYFVESLTDSLEADALKLIERIDSLGGAVTGIENGFQQREIQEAAYRLQMDMEKG
                     RRVIVGVNRFQTEEPPVEGLMRVDPRVGERQCAKIAKVRRERDEAQVRQVLVRIEQAA
                     RGTENMMPLFVEAVEAYATIGEICDVLRGVFGEQRDALVF"
     gene            2665..3123
                     /locus_tag="VIM45_02170"
     CDS             2665..3123
                     /locus_tag="VIM45_02170"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEY8820122.1"
                     /translation="MATRDSVLRELETNNTNLLQELQGMPEEKLKEPFLGTWSAREIL
                     VHLGSWYDMMGQAMERMGRGERPGPEGVDLSDSDGMNAKYVDAARGKSVAEVQKGLET
                     GLARFEGGVKALPEDRFAEGKTAMRILETMAGHPLEHIEGIRHWKSRGTA"
     gene            3158..3997
                     /gene="mce"
                     /locus_tag="VIM45_02175"
     CDS             3158..3997
                     /gene="mce"
                     /locus_tag="VIM45_02175"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HEY8820123.1"
                     /translation="MLTKIHHVGVVVRSADEALRFYRDVLGLPVAKDEVIEDQGVRGV
                     LLPVGDTEIELLEPTREGTGVARFLEKGEGMHHVCFETDDVGAELDAAKAKGLRLIDE
                     KPRQGLAGMIGFLHPAATRGVLVEFATPPHGAHGSPPGPLVRNFDHLVVATKDLDGGV
                     QTWKDNFGLNANVPSEIPALGIKNVVLPVAGAKAFVELVSPLGDGGAVEKFLTERGEG
                     LFLISIEVGNLDSTVAGLRDKGLRVGDPVVMAGGNRLAFLSPRNTNGVSIQLLERAGT
                     AAG"
     gene            4011..4418
                     /locus_tag="VIM45_02180"
     CDS             4011..4418
                     /locus_tag="VIM45_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HEY8820124.1"
                     /translation="MTNERKIRVLIAKPGLDGHDRGAKVVARALRDAGMEVIYTGIRQ
                     TPQMIAEAALQEDVDVVGLSILSGAHMELFPKIVEELRSRGVDDVLLFAGGIIPEEDT
                     PALEKIGFQKIFRPGTSTQDIIAYVRNTVGVKA"
     gene            4415..>5330
                     /gene="meaB"
                     /locus_tag="VIM45_02185"
     CDS             4415..>5330
                     /gene="meaB"
                     /locus_tag="VIM45_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HEY8820125.1"
                     /translation="MTESYTDRVLRGERRALSRVITWVQENTPQGREALRTLYRHTGH
                     AHTVGVTGSSGSGKSTLTGALAREARRRNRTVGIVAVDPSSPFTSGAILGDRIRMQDL
                     TSDPGVFLRSMATRGSLGGLTAMAADVMTVLDAAGKDMVLIETVGAGQDEVEIARTAQ
                     TTCLVLTPGTGDDIQTMKAGIMEIADILVVNKADLAGTDQVLSQLRAMLSYAEHGDWI
                     IPIVRVVANREEGVGELLDRIDEHRAYLESSGRLVGRQLERSRHQIVESVRAELVRRY
                     LTGDGAGQLEQLAQLVADRELDPHSAAEQ"
BASE COUNT         1080 a         1560 c         1761 g          929 t
ORIGIN      
        1 gcgcggctcg gctagcattt gattctggca tggtatccgg gccgggcggc tagctcagct
       61 ggttagagcg cttcgttcac accgaagagg tcactggttc gagtccagtg tcgcccaccg
      121 gacagtggcc gggcaaccgg caagtggcat aggccgaagt ggcggaaccg gcagacgcgc
      181 tacattcagg gtgtagtgag ctttacgctc gtgggagttc aaatctcccc ttcggcacca
      241 ggccccttca catttcctgc gctcgtagct cagttggata gagcgtcagt ttgcggaact
      301 gaaggtcgtg ggttcgagtc ccgccgagcg caccagtctg gcgcagcgat gatgaacgtt
      361 tgaagcggcc gaagggccgc ttttgcttat atccggtatg taccgaacga ggagccagat
      421 atgatccctg aaggaaccaa gccgaacgag gacgagcgct atctgtcgct cggcgccgtg
      481 gctggccgct tcctcgtccg catcgacgat gtccaccgct ggatcgccca gggcagactc
      541 aagaccgtaa ccgggccgcg gggaaagacg cttatccctg agagcgccct cctcgacttc
      601 caacggaccg cctatagacg tgaaaactac aacggtaccg accatgggct gcactcctgg
      661 ccgaccgaca tcccggaagt gtcgaacctg ccgatgatcc ccgtccagaa cttccagcag
      721 gctgaagcca acgcgccgat ctggccgttt ccgcacgcaa agcctagaat ggccgcaaag
      781 ggcgattagc tcagttggct agagcatctc gcttacaccg agagggtcag aggttcgagt
      841 cctctatcgc ccaccgcacc cgcacctctt tcatttgcag cggaccactt tttctagatg
      901 gttccgctgg ctgccaccgt cagtggggga tgagccgcca tgccaagcat gagtcttcac
      961 gaatggcgag aagcaaccta cgcgagggcc ctcaagcgtg gggccgagcg ccaaccggaa
     1021 tttgaaacga ccggcgcgat gtcgatcgct cccctctata cgcccgaaag cctgaacggc
     1081 tggaactacc aggagcaact gggatatccg ggcgagttcc cgtacacgcg cggcgtccag
     1141 ccgacgatgt accgtggccg cttctggacg atgcgccagt acgccggatt cgccgacgcc
     1201 gaggagtcaa accgtcggta ccgttacctg ctggagcagg gacagacggg gctatcggtc
     1261 gctttcgatc tgccgacgca gatcggctac gactccgatg acgagatggc ccagggcgag
     1321 gtcggcaagg tcggcgtcgc aattgacagc ctgctcgaca tggagatcct gttcaaccag
     1381 atcccgctgg aacaggtcac gacctcgatg accatcaacg cgaccgcgcc gatcttgctg
     1441 gcactgtacg tggccgtggc gcggaaacag ggtgctgacc tcacgaagct cggcggcacg
     1501 attcagaacg atattttgaa ggagtacgtc gcccgcggca cctacatctt cccgccgaag
     1561 ccgtcgatgc ggctgattac cgacgttttc gcatggtgta aagacaacct gcccgcctgg
     1621 aacacgatct cgatcagcgg ctatcacatg cgcgaggcgg gctctacggc ggcgcaagag
     1681 atcgccttca cgctcgccga tgctatcgct tacgttgagg ccgcccagaa tatcgggctg
     1741 aagttcgacg agttcgggcc gcgcctctcc ttctttttcg cctgccataa caacttcctc
     1801 gaggaagtgg ctaagttccg cgcggcgcgc cggctgtggg caaagatcgc gcgcggtcgt
     1861 ttcggtagta cagacccgcg atcgcagatg ctgcgcttcc atacgcagac cggcggcgca
     1921 acattgacgg cccagcagcc ggacaacaac atcgtgcgca cgacgatcca ggcattggcc
     1981 gcggtgctcg gcggaacaca gtctttgcat accaactcga aggacgaagc gctggcgttg
     2041 ccgagtgagc acgctgtgca gatcgcgctg cggacgcagc aggtgatcgc gtacgagtcc
     2101 ggctcgggcg acacggttga tccgctcgcc ggctcgtatt ttgtagaaag cctgaccgac
     2161 agcctcgagg cagatgcgct gaagctgatc gagcgcatcg actcactggg cggcgcggtc
     2221 accggcatcg aaaacggctt ccagcagcgt gagatccagg aggcggccta tcgcctgcag
     2281 atggacatgg agaaggggcg acgcgtaatc gtcggcgtca accgcttcca gacagaagag
     2341 ccgccggtcg aagggctgat gcgcgtcgat cctcgcgtgg gcgagcgcca gtgcgcgaaa
     2401 atcgcaaaag tacgccgcga gcgcgacgaa gcgcaggtac ggcaggtgct ggtgcgaatc
     2461 gagcaggcgg cccgcggtac cgaaaacatg atgcccctgt ttgtcgaagc cgtcgaagca
     2521 tatgcgacca taggagagat atgcgacgtg ctgcgcggcg tcttcggcga acagcgtgac
     2581 gcgctggtct tctaagttgg agtgtccata cccacaaatg tagtccagag cgaaacggct
     2641 ggtggtcaga taaggagggg cgcgatggca acccgcgatt cggttctccg agagcttgaa
     2701 accaacaaca ccaatctgct gcaagaattg cagggtatgc cagaggaaaa gctgaaggag
     2761 cccttcttgg gcacctggtc ggcgcgcgag attctggtac acctcggcag ctggtacgac
     2821 atgatgggcc aggcgatgga acggatgggc cgcggtgaac ggcccggacc agaaggagtg
     2881 gacctatccg actccgacgg gatgaacgca aaatacgtcg atgccgcacg gggcaagagc
     2941 gttgccgagg tacaaaaggg tctggaaacg gggttggccc ggtttgaggg cggtgtaaag
     3001 gcgttgccgg aggaccgttt tgccgaaggt aagacggcga tgcgcatcct ggagacgatg
     3061 gccggtcatc cgctcgagca tatcgagggt attcggcact ggaagagccg gggcactgca
     3121 tgagtttcgg tagccggcga ttgccggagg aggacagatg ctaacgaaga tccaccacgt
     3181 cggtgtggtc gtgcgcagcg ccgatgaggc gctgcgtttt taccgtgacg tgctcggcct
     3241 acccgttgca aaagacgagg tcatcgagga ccagggcgtg cgtggcgttc tcctgccggt
     3301 cggcgataca gagatcgagt tgctcgagcc aacacgggaa ggaaccggag tggcgcgctt
     3361 cctggaaaag ggcgagggca tgcatcacgt ctgttttgaa accgacgacg tcggcgccga
     3421 gcttgatgcg gcgaaggcga aggggctgcg tctgatcgac gaaaagccgc ggcaaggtct
     3481 ggccggcatg atcggctttc tgcacccggc cgcgacgaga ggcgtgttgg tcgagttcgc
     3541 gaccccgccg catggggccc acggttcgcc gcccggtccg ctggtgcgca acttcgacca
     3601 cctcgtcgtc gccacgaaag acctcgacgg gggcgtccag acatggaaag acaattttgg
     3661 cctcaacgcg aacgtgccaa gcgaaatacc cgcgcttggc atcaagaatg tggtgctgcc
     3721 ggtagcgggc gccaaggcgt tcgtcgagct tgtgagcccg ctcggtgacg gcggagcggt
     3781 agagaagttc ttgaccgagc gcggcgaagg gctgttcttg atttcaattg aagtcgggaa
     3841 cctggatagt acggtggctg gtctgcgcga caaggggttg cgcgtcggcg acccggtagt
     3901 catggcgggc ggcaaccggc tcgcatttct cagtccgcgc aacacgaatg gagtctcgat
     3961 tcaattgctc gaacgcgccg gcacggcggc aggctaggaa cgggagcgga atgacaaacg
     4021 aacggaagat cagggtgctc attgcgaagc cgggtctcga cggtcacgac cggggcgcca
     4081 aggtcgtggc gcgggccctg cgcgacgcgg gcatggaggt catttatacc ggcatccggc
     4141 agacgccgca gatgatcgcc gaggccgcat tgcaagagga cgtcgacgtg gtcgggctca
     4201 gcattctgtc aggcgcccac atggagctgt tcccgaagat cgtcgaagag ctgcggtcgc
     4261 gcggtgtgga tgatgtattg ctgttcgctg gcggcatcat tccggaagag gacacgccgg
     4321 cgctggagaa gattggcttc cagaagatat tccgtccggg aacctcaacc caggacatca
     4381 tcgcgtacgt gcgaaacacg gtcggtgtga aggcgtgacc gagagctaca ccgatcgcgt
     4441 cctcagaggt gaacggcggg cgctttcgcg cgtcattacg tgggtacagg agaatactcc
     4501 gcagggtcgc gaggcgctgc gcacgctgta ccgccatacc ggtcatgcac atacggtcgg
     4561 agtgacgggc tcttccgggt ccggaaaaag tacgctcacc ggcgcgctgg cccgcgaagc
     4621 gcgacgccgc aaccgcacag tcggtatcgt tgcggtcgac ccgtcaagtc ctttcaccag
     4681 cggcgccatt ctgggtgacc gcatccgcat gcaggatcta accagcgatc ccggcgtttt
     4741 cctgcgcagc atggcgactc gcgggtcgtt gggcggtctg acggcgatgg ccgccgacgt
     4801 gatgaccgtg ctcgacgctg ccggcaagga tatggtgcta attgaaaccg tcggtgccgg
     4861 gcaggacgag gttgagatcg cgagaaccgc ccagacgacg tgcctggtcc tgacgccggg
     4921 tacgggggac gacatccaga cgatgaaggc cggcatcatg gagatagccg acattcttgt
     4981 cgtgaacaag gccgatctcg cgggcaccga ccaggtgctg agtcagctgc gcgccatgct
     5041 ctcttacgcc gagcacggcg attggatcat cccgatcgtg cgggtcgtgg ccaatcgcga
     5101 agaaggggtc ggcgagctgc tcgatcgtat cgacgagcac agggcatatc tcgaaagcag
     5161 cggcaggctc gtcggccggc agttagaacg atcgcggcat cagatcgtcg agtcggtgcg
     5221 cgccgaactg gtccgccgct acctcacggg tgacggcgcc ggccagctcg agcagctggc
     5281 gcagttggtc gctgaccgcg agcttgatcc gcactcggct gccgaacaga
//
LOCUS       DATCPE010000069         5006 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U7219
            k141_465517, whole genome shotgun sequence.
ACCESSION   DATCPE010000069 DATCPE010000000
VERSION     DATCPE010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955033
            Sequence Read Archive: SRR1586279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 5006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 16:49:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 640
            CDSs (total)                      :: 631
            Genes (coding)                    :: 627
            CDSs (with protein)               :: 627
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5006
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_465517"
                     /isolate="SMAG_U7219"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="VIM45_02190"
     CDS             complement(<1..288)
                     /locus_tag="VIM45_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEY8820126.1"
                     /translation="MVRQIKIAPSILAADFARLGEQVRAAEEAGADYIHVDVMDGQFV
                     PNITLGPLIADAVHRSTSLPLDLHLMILQPDRQINAFREAGASILTVHAEAC"
     gene            complement(281..1786)
                     /gene="argH"
                     /locus_tag="VIM45_02195"
     CDS             complement(281..1786)
                     /gene="argH"
                     /locus_tag="VIM45_02195"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013639091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEY8820127.1"
                     /translation="MRKRTGSDGDKRWAGRFEKQTNPLVEAYTSSIAQDEALLAYDIA
                     GSIAHATMLGQQEIIDPLDAQRIVKGLQSIAADAAAGAFQMRPELEDVHMNVEAELER
                     RIGPAAGRLHTARSRNDQVVTDFRLLVRNATHQAITAIVELQRVLIDLAEQHIDVVMP
                     GYTHMQLAQPVRLSHHLMAYFEMFRRDCTRFAAVGNSIDESPLGAGALAGLPYPLDRE
                     GAAEALGLARVTANSMDAVSNRDFVIDFHHAAAVCMMHISRMAEEIIVWSTREFGFIT
                     LDDAFATGSSIMPQKKNPDVAELARGRTGLVYGNLIAALTMMKGLPLTYNRDLQEDKG
                     PLLNSAKTLIATVNIFAAMLPTITWNVDRLREAAGSGYSLATDIADYLVRKQMPFREA
                     HGVVGRLVRYAESRTKDFGDLTLDEYRQFSQLFDKSVLSIDLNSAIDARDVIGGTARN
                     RVLDQIDRARRWFIEGFESMEPSTPTARNARSKTKSRSTGRRTVAKRSSNG"
     gene            1978..3561
                     /locus_tag="VIM45_02200"
     CDS             1978..3561
                     /locus_tag="VIM45_02200"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010632018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HEY8820128.1"
                     /translation="MEVPLLVSDFLRRAVEVYPEKEGVVCGGARFTYAEFGSRVNRLA
                     NALAAMNVAKGDRIAILSPNCHRFLEAFYGVTAVGAVLVPLNYRLVPSDFTYIVNHAG
                     ASVFLVDEELVPVADEIRPQLGTVREYVMLGGSTKEGWTSYETMLADATDGTPADACV
                     DENDVATINYTSGTTARPKGVMMTHRNLYANAWNTIGHIRVSHDDVLIHTLPMFHANG
                     WGTPFSGTGMGVKHVVLRKVDGADIFRLIQDESVTWACMAPAVLSVILNFPDKQNYKI
                     TTAPRLVVAGAPPPAAFIKRLRQELGWDFIQVYGLTETAPFLTITILRPDMYSLSIDE
                     QCQIQARAGVPMVGVDLRIVDQHDQDVVPDDEQVGEVIARSNVVMKGYWRQPGETGKA
                     IVNGWFHTGDLATINKAGYINIVDRAKDVIISGGENISSIEVEDALYKHPAVLECAVI
                     GVPSEKWGETPKALIVPREGMQPDETEIIGFCKEQMAHFKAPTSVELVESLPRTATGK
                     LQKYVLRDKYWGGAKRKVN"
     gene            complement(4319..>5006)
                     /locus_tag="VIM45_02205"
     CDS             complement(4319..>5006)
                     /locus_tag="VIM45_02205"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY8820129.1"
                     /translation="EALRAAVTPKTCAIMLEPVQGEGGVNLPSENYLQAVRKLCDDQN
                     ILLVLDEIQTGVGRTGRLWAYEHFGIEPDVMTLAKGLAGGVPIGAILAKEVVAACFVP
                     GDHGSTFGGNPLATAAGFSVLSYIIDNDLPADVLRKGDKLMQRLHGIEDRHRDISEVR
                     GMGLLCAVEFSGDLADGVTRGCMDRGLLINILRPNTVRFSPPLTVSDQEIDEALDIFE
                     SVLSELPTNK"
BASE COUNT          969 a         1477 c         1557 g         1003 t
ORIGIN      
        1 gcaggcttcg gcatggaccg tcaggatgga ggctccggct tctctgaagg cgttgatctg
       61 gcgatcgggt tgaaggatca tcaggtgtag gtcgagcggg agcgaagtac tgcggtggac
      121 ggcatccgcg attagcggcc caagggtgat gttgggcacg aactgaccgt ccattacatc
      181 gacatggatg tagtcggcac ccgcctcctc cgccgcgcgc acctgctctc cgagtcgggc
      241 aaagtccgcg gcgaggatcg acggcgcaat ttttatctgt ctaaccattg gacgatctct
      301 tcgccaccgt tcgtcggccg gtagaccgtg acttcgtctt actcctcgca ttgcgggccg
      361 tcggcgtgga cggttccata gattcgaaac cttcgataaa ccaccgtctg gcacggtcga
      421 tctgatcgag aacgcggttg cgtgccgtac cgccgatcac gtcgcgggca tcgatcgcgc
      481 tgttcaggtc gatgctcagc actgatttgt caaatagttg cgaaaattga cggtactcgt
      541 ccaaagtgag gtcgccgaag tccttcgttc gtgattcggc gtagcggacc agtctgccga
      601 ccacgccatg cgcttcgcgg aacggcatct gcttgcgtac cagataatcg gcgatgtcgg
      661 ttgccagcga gtagccgctc cctgcggcct cgcgcagccg gtcgacgttc catgtgatcg
      721 tcggcagcat cgcggcaaag atgttcaccg ttgctatcag cgtcttcgcc gaattcagga
      781 ggggcccctt atcctcctgc aggtcgcggt tgtaggtcag tggcaggcct ttcatcattg
      841 tgagcgcggc tatcaagttg ccgtacacca ggccggtgcg gccccgcgcc agttcggcga
      901 cgtcgggatt cttcttctgc ggcatgatgc tcgaacccgt tgcgaaggca tcgtccagtg
      961 tgatgaaacc gaattcacgg gttgaccaga cgatgatctc ttcggccatc cgcgagatgt
     1021 gcatcatgca gacagcggcg gcgtggtgga agtcgataac gaagtcacgg ttcgagaccg
     1081 cgtccatgct attggcagta actcgggcga ggccgagcgc ctcagccgcg ccttcgcggt
     1141 cgagcgggta tggaagaccc gccaaggctc ccgctccgag cggcgattca tcaatcgagt
     1201 tgccgaccgc ggcgaagcgg gtacagtcac gccggaacat ttcgaagtag gccatcaggt
     1261 gatgtgaaag ccgtacaggc tgggccaact gcatgtgcgt gtagcccggc atgaccacgt
     1321 cgatgtgctg ttcggcgaga tcgatgagca ctctttgcag ttctacgatc gcagtgatcg
     1381 cctggtgggt cgcattgcgc acgagcaggc gaaaatcggt gacgacctgg tcgtttcgag
     1441 accgagcggt gtgaagccgt ccggccgcgg gaccgatacg acgctcgagc tcagcctcga
     1501 cgttcatgtg gacgtcttcg agttcgggcc gcatctggaa agcgccggct gctgcgtcgg
     1561 cggcgatgct ttgcaggccc ttcacgatgc gctgggcatc cagcggatcg atgatttcct
     1621 gctgtccgag catcgtggcg tgggcgatcg agcccgctat gtcgtaagcg aggagcgctt
     1681 cgtcctgggc aattgacgaa gtgtatgcct cgaccagcgg gttggtctgc ttctcaaacc
     1741 gtccggccca gcgcttgtca ccgtcggagc cggtcctttt gcgtatccca tccccaccgg
     1801 atgcggcctt cgtgttacgg ggcaatgctc acctcaccgg cagatatggc gggttcagga
     1861 tagcgccgct cgcggcagga caccacttga cgtactgttt gcagcgcata gtctaggagg
     1921 cagggcggcc gggggtgcct ggccgccggc cgatgatttg catttagggg gttgattgtg
     1981 gaagtcccac tgcttgtatc ggactttttg cgccgtgcgg tcgaggtgta tccagagaag
     2041 gaaggtgtcg tctgcggcgg cgctcgcttt acctacgcgg agttcggaag ccgtgtgaac
     2101 cggctcgcga acgcgctcgc ggcgatgaac gtggccaaag gcgaccgcat cgccatcctc
     2161 agcccaaact gccaccggtt cctcgaggcg ttttatggag tgacggcggt cggcgccgtt
     2221 cttgttccgc tgaactaccg tctggtgccg tccgatttca cgtatatcgt caatcatgcc
     2281 ggcgcttcag ttttcctcgt cgacgaagag ctcgtgcccg ttgccgatga gattcgacca
     2341 caactgggta ctgtgcgcga atacgtcatg ctgggtggct cgaccaaaga gggctggact
     2401 tcctacgaaa cgatgctggc ggatgcgacc gatggaacac ccgccgatgc ttgcgtcgac
     2461 gaaaacgacg tggcgaccat caactacacc tcgggcacca cggcacggcc caagggcgtg
     2521 atgatgaccc accgcaacct gtatgcaaac gcgtggaata ccatcggaca cattcgcgtg
     2581 tcacacgacg acgtgctgat ccatactctg cccatgttcc acgccaacgg ttggggcacg
     2641 ccgttttccg gcacgggtat gggggtcaag cacgtggtgc tgcggaaggt cgacggggcc
     2701 gacatcttcc gcttgatcca ggacgagagc gtaacctggg cgtgcatggc acccgccgtc
     2761 ctgagcgtga tcctcaattt ccccgataag cagaattata agatcaccac ggcgccacgg
     2821 ctggttgtcg ccggtgcgcc gccgccggcc gcattcatca agcgtctgcg ccaggagctc
     2881 ggttgggatt tcattcaggt ctatgggctc acggaaaccg cgccgttcct gacgattacg
     2941 atcctgcgcc ccgacatgta ctcgctctcg atcgacgagc aatgccagat ccaggcccgc
     3001 gcgggggtgc ccatggttgg cgtcgacctt cgtatcgtcg accaacacga ccaggacgtg
     3061 gtaccggatg acgagcaggt cggagaagtg atcgctcgtt caaacgtcgt gatgaagggg
     3121 tactggcgcc aaccgggcga aacaggcaag gcaatcgtaa acggctggtt tcacaccggc
     3181 gacctcgcga cgattaacaa agcgggctat atcaacatcg tcgaccgcgc taaggacgtg
     3241 atcatttcag gtggagagaa catctcatcg atagaggtag aggacgcact ctacaagcat
     3301 ccggcggtac ttgagtgcgc tgtcatcggc gtgccaagcg aaaaatgggg cgagacgccc
     3361 aaggcgctga ttgtgcctcg cgagggcatg cagccggacg agacagagat catcggtttc
     3421 tgcaaggagc agatggccca tttcaaggct cccacctcgg tcgagctggt cgagagcctg
     3481 ccgcgcaccg caaccggtaa gctccagaag tacgtactgc gcgataagta ctggggcggc
     3541 gccaagcgca aagtgaacta acggttgccg ctgcccgctt aaacagcaca agagcccgct
     3601 tcgcccggtt acgcctcggt cttcccagag actagccaag gcggcatcgc gtgaccccgt
     3661 cgtgggcgtg ataaaggccc ataggtacgt gctattcttc atgtgaccga ttgatcgcag
     3721 gaatggtcct atgaccgcgg tagaagaacg cgtccgtact cacgtagtcc tcccgcgcgg
     3781 cgttattgaa gctgttgatc ggattgcggg aagacgtaat cgcagcgaat tcatcgccgc
     3841 ggcggtccgg gacagtctaa tacgggcact gcaaagggaa gcgctggaga atacggccgg
     3901 ggtgctagcc gggagcgact atcccgaatg ggagacgcca gaaaagacat ccgcctgggt
     3961 tcgtaagttg cgagcggaag acaacaggcg tctcgaaagg aagctgcgtc gctggtctag
     4021 ttcatgagct ggcacgttct cgccacaacg gacgtggtaa tagcggagac atggtccggt
     4081 ctacgtccag aagaacgtgc tacaggagag gaactcctct cgtctttcgt attcttgccg
     4141 acgtcccggg aagcggcgcg tcgcgcgggg gaatggcgat acaccgcccg agatcaacat
     4201 tgttccgctc ccgcgcgcgg cgtggaactc cataacgcgt cgccgggtcg tcggctccct
     4261 ccgtcttccg tgtgcacgcg ggccacttag ctcaccacgt gtctggtgcc agcgccggtc
     4321 acttattcgt cggtagttcg ctgagcaccg actcgaaaat atcgagcgct tcgtcgatct
     4381 cctggtccga aaccgtgagc ggtggtgaga agcggaccgt gttcggacgc agtatgttga
     4441 tcaagagacc acggtccatg cagccgcggg tcacaccgtc cgctaaatcg cccgagaatt
     4501 ccacggcgca aagcagcccc atgccccgaa cttcgctgat atcgcgatga cggtcctcga
     4561 tgccgtgcag ccgttgcatc agcttgtcgc ctttgcgcag cacgtcagcg ggcaggtcgt
     4621 tgtcgatgat gtaactcagc accgaaaagc ctgctgcggt agcgagcggg ttgccaccaa
     4681 acgtcgatcc gtggtcgccg gggacgaagc acgcggcaac cacctctttt gcgagaatag
     4741 cgccgatcgg aacacctccg gcaagtccct tcgccagggt catgacgtcc ggctcgatgc
     4801 cgaaatgctc gtatgcccat agccgcccgg tacggccaac gcccgtctgg atctcgtcca
     4861 aaaccaggag aatgttttga tcgtcgcaaa gtttgcgcac ggcctgcaga tagttttctg
     4921 agggcaggtt taccccgcct tcgccttgca cgggctccag catgatggcg cacgtttttg
     4981 gtgtcaccgc tgcccgaagc gcctcg
//
LOCUS       DATCPE010000070         5873 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U7219
            k141_77578, whole genome shotgun sequence.
ACCESSION   DATCPE010000070 DATCPE010000000
VERSION     DATCPE010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955033
            Sequence Read Archive: SRR1586279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 5873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 16:49:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 640
            CDSs (total)                      :: 631
            Genes (coding)                    :: 627
            CDSs (with protein)               :: 627
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5873
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77578"
                     /isolate="SMAG_U7219"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..563
                     /locus_tag="VIM45_02210"
     CDS             <1..563
                     /locus_tag="VIM45_02210"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEY8820130.1"
                     /translation="CGMVISAKTYEQVALEDPNGHWELDHGCLRQKPGMTAQHDFLMQ
                     TLTAELMGQLDRSLFLVYMNTMRLRTSDGSFYVPDVTVVPRASVQAALDLRSTRLDVY
                     TEAMPLVVEVWSRSTGGYDVETKLLEYQLRGDAEIWLLHPYDRTLTAWRLQADGRYTE
                     SVHREGVIEPVALPGVRIELAALFEA"
     gene            592..1098
                     /gene="ybeY"
                     /locus_tag="VIM45_02215"
     CDS             592..1098
                     /gene="ybeY"
                     /locus_tag="VIM45_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEY8820131.1"
                     /translation="MAANEISLIVDERYRLPGAEVWLPALAERVLQSEQIYDSSLSVL
                     ITDDETVHQLNRTYRGYDQPTDVLSFGLSELAKPAIDGEVAESGFVLPPDSCSQLGEV
                     IISLPTAQRQAREHDRPVEMELAHLLIHGVLHLLGYDHYEPDEEREMRSREEKLLAER
                     TWEPKEYR"
     gene            1155..2882
                     /gene="dnaX"
                     /locus_tag="VIM45_02220"
     CDS             1155..2882
                     /gene="dnaX"
                     /locus_tag="VIM45_02220"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEY8820132.1"
                     /translation="MSAEVWSRKWRPRTFNEVVGQKHVTQTLARAVALDRVAHAYLFC
                     GPRGTGKTSTARILAKAVNCEANRHESGGPQPGRSRGQPCDECDSCRSVTEGRALDLI
                     EMDAASNRGIDEIRSLRDKVGYSPNSSRYKVYLIDEVHELTQFAFDALLKTLEEPPAH
                     VLFVLATTEAHKVPETILSRCQRFDFTRIKLADIVARLRQIAEAEGIEITSGALELIA
                     RRATGSLRDAENLFEQTVAAHGNVLTEDNIRGGAAATGDQRATAVVKAALQRRLADAL
                     NLVAEVRDDGDDLRQFTAAVVQHLRSVLLVRAGAEGSLALSADAIEALRVVAAETPVI
                     EVLRLAKSLSATDFRADPQSPLPLELALIEFVETPVLAPPAQKAGQPGNEEARPKPTG
                     LQPSGAVDRIRSAITPRSGPPPLARATAVEVVPSARSNGTVGAVGTPASVSDSDSARL
                     VARQSGAPAAVTLEDAQRRFRDIYERCSELDRSAARLLNSGGCDIVGIDAAVVTIGMR
                     HPRMVTLAAAVASTIRRSVNEILGGEYTVTYTHVPDVPSRLQLSAAEQPSHLLDEALK
                     LGAKPVGRA"
     gene            2926..3288
                     /locus_tag="VIM45_02225"
     CDS             2926..3288
                     /locus_tag="VIM45_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009165817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEY8820133.1"
                     /translation="MNRAQRRSGGGGGAGGFGGINPDMLRRAQELQNKLLQAQEEAAL
                     AVTEGTSGGGVVRAQVKGDMKVIGISIEKDVVDPEDVDMLQDLILAAIKDAQDRIEAK
                     QAETMGSLTGGLKIPGLL"
     gene            3391..4002
                     /gene="recR"
                     /locus_tag="VIM45_02230"
     CDS             3391..4002
                     /gene="recR"
                     /locus_tag="VIM45_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008476413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HEY8820134.1"
                     /translation="MSNAASLAAAPVARLIDEFHKLPGVGPKTAQRLAYYILRTGPDD
                     AHALAQAIIDAKEQITFCSSCQNMTDVEPCSICRSDKRDAATICVVEEPLDILAIERT
                     RSYSGLYHVLHGVISPMDGIGPDDLKVRELIGRLRDGSVHEVIMATNPNIEGDATAMY
                     LERLITPLDVRVTRLARGLAMGTDLEYADEVTLTRAFEGRQLV"
     gene            4020..4853
                     /gene="recO"
                     /locus_tag="VIM45_02235"
     CDS             4020..4853
                     /gene="recO"
                     /locus_tag="VIM45_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008478588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEY8820135.1"
                     /translation="MAGRIPRVYRAEAVVLRQRRLGEADRIVTLFTPQLGKIDAVAKG
                     IRKQTSRKAGHLEQLSLTSLLLASGKNLDIITQGETIEGFMPLRQDLERLARGLYVAE
                     LVDRFSELRAENYPVFRLLVETLQRLSSTVAVELAVRYFELHLLSHLGYRPELGRCVA
                     CRADLEPVENSFSAALGGVVCPECRYSNVGLWRLSVNALKVLRLLERGSFEDAERLRL
                     TPELSMELELLLQGYVRHVLERNPRSLEFVDAVRRTHDSRLKPALALVAESAAAYEPG
                     N"
     gene            4953..5384
                     /locus_tag="VIM45_02240"
     CDS             4953..5384
                     /locus_tag="VIM45_02240"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEY8820136.1"
                     /translation="MPVYEFYCEPCVGVFEVLRPIREASDAASCPVCSSDARRMMPTS
                     FNAFTFRDGYPRRIPDKGTYWHLGKEVKNLVTGPVRPNEHPEINKPEPRRRPLKGDQI
                     DAQEAGRMQAQAKAIEERERQSAIQAYEKTQAKKSKTADTV"
     gene            complement(5388..>5873)
                     /locus_tag="VIM45_02245"
     CDS             complement(5388..>5873)
                     /locus_tag="VIM45_02245"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEY8820137.1"
                     /translation="DYFGRIAGKTASLFGTACEGGAIASACPEPWIDALREYGFNLGM
                     AFQIVDDILDYTGNQAIMGKPVGSDLLSGTLTLPALLLIERTEGENPVRRLFLARRNR
                     QQRLAEVLEAVHSSGALRDAEAVARDFMNRGLEAIAPLSNGEAKTALIDLSEYVLTRA
                     S"
BASE COUNT         1163 a         1751 c         1908 g         1051 t
ORIGIN      
        1 ggtgcggaat ggtcatcagt gcgaaaacgt acgagcaggt agcgctggag gatcccaatg
       61 ggcactggga gcttgaccat ggctgcctcc ggcagaaacc gggtatgacc gcccagcatg
      121 acttcctaat gcagacactc acggccgaac taatggggca gcttgaccgc agtctgttcc
      181 tggtctacat gaacacgatg cgcctgcgga catcggatgg ctcgttctat gtcccagatg
      241 tcaccgttgt ccctagagca tcggtacagg cggcgctcga cttacgcagt actcgactcg
      301 atgtgtacac agaagcaatg ccattggttg tcgaggtttg gtcacgttcg accggcggat
      361 acgatgtcga gacgaagcta ttggaatacc agctgcgcgg agacgccgag atctggctcc
      421 tgcacccgta tgaccgcacg ctgaccgcgt ggcggctgca agccgacggt cgttacaccg
      481 agtccgtcca ccgcgaaggc gtcatcgagc cggtcgcgct gcccggcgtc cgcatcgaac
      541 tagcggcact gttcgaggcg tagtctgcag tccaagctag aagatggacc attggcggcc
      601 aacgagatat cactcatcgt cgacgaacgg tatcgtttac cgggcgcgga ggtctggctg
      661 ccggcgctcg ccgagcgggt gctgcagagt gagcagatct acgactccag cctatctgtg
      721 ctcataacag acgacgagac agttcaccaa ctgaaccgga cgtatcgggg ctatgaccag
      781 cccacggacg tgctctcgtt cgggctctcg gaactggcga agccggccat agacggagaa
      841 gtcgccgaat ccggcttcgt cctaccgccc gatagctgtt cgcaacttgg agaggtgatc
      901 atctccctgc cgacggcgca acgccaggcg cgcgagcacg accgaccggt cgaaatggag
      961 ttagcacacc tgttgatcca cggcgtgctg cacttgctcg gctacgacca ttacgagccc
     1021 gatgaagagc gcgaaatgcg ctcacgcgag gaaaaacttc tcgcggagcg cacctgggag
     1081 ccgaaggaat atcggtagcc gggcccacca ctgtaaccct ataatgacac acgtgttcca
     1141 atggggtgca gggcgtgagc gcagaagtct ggtctcgaaa gtggcgaccg cggacgttca
     1201 acgaggttgt cggccagaag cacgtgaccc agacgttggc ccgcgccgtt gcgttggaca
     1261 gggtcgcgca cgcgtacctg ttctgcggcc cgcggggaac gggcaagacc agcaccgctc
     1321 ggatcctcgc caaagcggtg aactgcgagg caaaccggca cgagagcggc gggccgcagc
     1381 cagggcgctc tcgcggccag ccgtgtgacg agtgcgattc atgccgctcc gttacggagg
     1441 gccgggcgct cgatctgatc gaaatggacg ccgccagcaa ccgcggcatc gacgagatcc
     1501 gcagcctgcg tgacaaagtc ggctactctc ccaactcgtc gcgttacaag gtctacttga
     1561 tcgacgaggt gcacgaacta acccagttcg ccttcgacgc actgctgaag acgctggaag
     1621 agccgccggc tcacgtgctg ttcgtgctgg cgacgaccga agcacacaag gtgccggaga
     1681 cgatcctgtc gcgctgtcag cgcttcgatt tcacacggat caagctcgcg gacatcgtcg
     1741 cgcggctgcg tcagatcgcc gaagctgaag gcatcgagat taccagcggt gccctcgagc
     1801 tcatcgctcg tcgggcaaca ggtagcctgc gcgacgccga gaacctgttt gagcagacgg
     1861 tggccgcaca cgggaatgtg ctcaccgaag ataatattcg tggcggcgcc gccgcgaccg
     1921 gcgatcaacg cgctaccgct gtggtaaaag ccgcgctgca gcgccggctc gctgacgcgt
     1981 tgaacctggt ggcggaggtc agggacgacg gcgatgacct gcgccaattc accgcggctg
     2041 tagttcagca cctgcgttcg gttctgctcg tgcgcgccgg agcggagggc tcgctcgcgt
     2101 tgagtgccga tgcaattgag gcgcttcgcg tggttgccgc tgagacgccg gtgattgaag
     2161 tgttgcggct cgccaagtca ctgtcggcca ccgacttccg cgccgacccg cagtcgccgc
     2221 tgccgctcga gctggcgttg atcgagttcg tcgagactcc ggtgctcgcg cctccggctc
     2281 aaaaagcggg tcagccgggg aacgaagagg cgcgccccaa gccgacgggt ttacagcctt
     2341 cgggcgccgt cgaccgcata cggtctgcta tcacgccgcg gagcggaccg ccgccgctgg
     2401 cgcgtgccac cgctgtcgag gtagtaccgt ccgcgagaag taatggcact gtcggcgcgg
     2461 tcggaacccc agcatccgta tccgattcag acagtgcgag gttggttgcc cggcaaagtg
     2521 gcgcgcctgc agcggtaacc ctcgaagacg ctcagcggcg cttccgtgac atttacgaac
     2581 gctgcagtga gctcgacaga agcgccgcgc ggctattgaa cagcggcggg tgtgatatcg
     2641 tcgggattga cgcggcggtt gtgacgatcg gtatgaggca tccgcgaatg gtgacgctgg
     2701 cagccgcggt cgcatcgacc atacgaagga gcgtgaacga gatcctcggc ggagagtaca
     2761 cggtgaccta cacgcacgtc ccggatgtgc ccagccgcct gcagctttcg gccgccgagc
     2821 aaccgagcca cctgctggac gaagcgctga agttgggagc aaagccggtg gggcgcgcct
     2881 gaaagtgccc cgcgaaccga tcggcaagtt acagggaggg aaccgatgaa ccgagcacaa
     2941 cggcggtccg gcggcggcgg cggggccggc ggtttcggcg gtataaatcc cgacatgttg
     3001 aggcgggccc aggagcttca aaacaagctg ctccaggccc aggaggaggc tgccttagca
     3061 gtgaccgagg ggacttctgg cggcggcgtg gtgcgcgccc aggtcaaggg cgacatgaag
     3121 gtcatcggta tttcgatcga gaaggacgtc gtagaccccg aagacgtgga tatgctccag
     3181 gatctgatcc ttgccgcgat caaagacgcg caggaccgga tcgaggcgaa gcaggctgag
     3241 acaatgggtt cgttgaccgg cggccttaag atccctggac tgctctaagg ggtggcagtg
     3301 gcgatggccg gcagttgcgc tttcccgatg ctgtgactgt gccttagcag gtttggtata
     3361 ataagtacag tcattgagag gtgaatttgc ttgtccaatg ccgcttctct cgcggccgcc
     3421 ccggttgcga gactaattga cgaatttcat aaattgccgg gagtaggtcc taaaaccgct
     3481 caacgtcttg cttattacat cctacgaaca ggtccggacg acgcgcacgc gcttgcgcag
     3541 gcaattatcg acgcaaaaga gcaaataacg ttttgctcgt cctgccagaa catgaccgac
     3601 gtcgagccgt gcagcatctg ccgctccgat aagcgtgatg ccgccacgat ttgcgtggtt
     3661 gaagagccgc tcgacatcct cgcgatcgaa cggacgcgca gctactcggg gctttatcat
     3721 gtcctgcacg gcgtcatctc gccgatggac gggattggac ctgatgacct gaaggtgcgt
     3781 gagttgatcg gccgcctccg ggatggctct gtgcacgagg tcatcatggc gaccaacccg
     3841 aacatcgagg gcgatgcgac ggcgatgtat ctggagcggc tgattacgcc gctggacgtg
     3901 cgtgtaacgc gcctcgcgcg cggtctggcg atgggcaccg atctcgagta cgccgacgaa
     3961 gtgacgctga cgagggcgtt tgagggccgg cagctagtct aggaatgcgg taaccgaacg
     4021 tggctggaag aattccgcgg gtctatcggg ccgaagctgt cgtgctgcgc caacgccgcc
     4081 tgggtgaggc cgaccgcatc gtcaccctat tcacgccgca gctcggcaaa atcgatgcgg
     4141 tcgccaaggg tatccgtaag cagacgagcc gcaaggcggg ccacctggag cagttgagcc
     4201 tgacgtcgct gttgcttgcg agcggtaaga acctcgacat catcacccag ggagagacga
     4261 tcgaagggtt tatgccgctg aggcaggacc ttgagcggct tgcccgcggc ctctacgtcg
     4321 ccgagctcgt cgaccgcttc agcgagctgc gcgcagagaa ctacccggtc ttccgcttgc
     4381 tcgtggaaac attgcagcgg ctgtcgagca ccgtcgcagt cgagctggcg gttcgatact
     4441 tcgagctgca cctgctgtcg cacctcggct accggcccga gctcggtcgc tgcgtggcgt
     4501 gcagggccga cctcgaaccc gtggagaatt cgttcagcgc cgcgctgggc ggcgtcgttt
     4561 gtcccgagtg cagatactcg aacgtaggtc tttggcgcct gagcgtcaac gcgctcaagg
     4621 tattacgcct gctagagcgt ggctcgtttg aggacgcgga acggctgcgg ttgactcctg
     4681 aactgtcgat ggagctggag ctgttgctgc agggatacgt acggcacgtg ctggagcgga
     4741 atccacggtc gctggaattc gtcgatgcgg ttcggcggac acacgactcg cggctgaagc
     4801 ccgcgctggc actggtcgcc gagtccgccg ccgcgtacga accgggcaac tgacacaatg
     4861 gttggacgcc atcccgcatg ataggatgaa gcgcgccgcg gtaatctatt ctgccccgta
     4921 ctgccgcggc ggacttgggg ttgaggtggt ttgtgccggt ttacgagttc tattgcgagc
     4981 catgtgtcgg cgtctttgaa gtattacgac cgattcgcga ggcgtcggat gcggcgtcat
     5041 gtccggtgtg cagcagcgat gccagacgga tgatgccgac atcgttcaac gcgtttacgt
     5101 tccgcgatgg gtatccgcgc cgcattcccg ataagggcac gtattggcac cttggcaagg
     5161 aagtaaagaa ccttgtcacg ggtcccgtgc gtcccaacga gcatccggag attaacaagc
     5221 cggagcctag gcggaggccg ctcaaaggcg accagataga cgcgcaagaa gcgggccgga
     5281 tgcaagctca agcgaaggcg atagaggagc gagagcgaca gtcggcgatc caggcctacg
     5341 agaaaaccca ggccaagaag agcaaaaccg cagacacggt gtagctatca actcgcccgg
     5401 gtcagcacgt attcggagag atcaataagg gccgtcttgg cttcgccatt tgaaagcggc
     5461 gcgatcgctt cgagaccgcg attcatgaag tctcgcgcga cggcttcggc gtctcgaagc
     5521 gcgcccgacg agtgcacggc ctctagcacc tcagccagcc gctgctgacg gttacggcgc
     5581 gccaggaaca acctgcgaac tggattttcg ccttcggtcc gctcgatgag cagcagcgcc
     5641 ggcagtgtca gcgtgccgga cagcaggtcg cttccgaccg gcttgcccat gatcgcctga
     5701 ttgcccgtgt aatcgaggat gtcgtcaacg atctggaacg ccatccccag gttgaatccg
     5761 tattcccgca aggcgtcgat ccacggctcc gggcacgcac tcgcgatcgc accgccttca
     5821 cacgccgtac cgaacagcga tgcggtcttg ccggctatac ggccgaaata gtc
//
LOCUS       DATCPE010000071         4310 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U7219
            k141_271939, whole genome shotgun sequence.
ACCESSION   DATCPE010000071 DATCPE010000000
VERSION     DATCPE010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955033
            Sequence Read Archive: SRR1586279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 4310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 16:49:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 640
            CDSs (total)                      :: 631
            Genes (coding)                    :: 627
            CDSs (with protein)               :: 627
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4310
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271939"
                     /isolate="SMAG_U7219"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="VIM45_02250"
     CDS             complement(<1..218)
                     /locus_tag="VIM45_02250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8820138.1"
                     /translation="MQQTNIMEQPVSEEVEGAPELPVCRHHWRIETPNGSTSVGKCKV
                     CGEIREFSNSSSDSIWENDSSDSGSRWRG"
     gene            454..1167
                     /locus_tag="VIM45_02255"
     CDS             454..1167
                     /locus_tag="VIM45_02255"
                     /inference="COORDINATES: protein motif:HMM:NF013209.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEY8820139.1"
                     /translation="MHSSKSQILVALKRQGRCSVDELAIALHLAPMTVRQHLATLERD
                     GLVVSVEERQRMGRPHFVFSLTEQGDESFGMRYDRIALQLLLEVGRLDSQEIAGLSAE
                     EKTALLFDRLADRFISRYRPRMGPLTLPQRIAAVSDALHTESGFTEWAATPEGYEIRD
                     YNCFFDRLNGTTANHCRWHERILTELLETPVECRSDAPPSVQLCHFVIKSPNTRAGAR
                     PMAAVLSMAGAHREPSATG"
     gene            1179..2273
                     /locus_tag="VIM45_02260"
     CDS             1179..2273
                     /locus_tag="VIM45_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018195602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HEY8820140.1"
                     /translation="MTQVSSIQEGGAIKDAVLAALSTVQDPELHRDLVSLGMIEDLAV
                     DGGSVSFKVVLTTPACPVRGEIEQQVEQAVMAVPGVRSVHVVMGAQVRSTPIKPGGPK
                     PIEGVRNVIAVASNKGGVGKSTVSVNLAVALAQAGARVGLADADITGPNIPTMMGFEA
                     GFQSQGNQGLRAVEKYGVKVASLGFLLPKGMPVVWRGPMIGTAVRQLLHDIPWGEGGD
                     LDYLIIDLPPGTSDASMSMAQDAAISGAVIVSTPQDVALEDALKAVGMFEKLHVPVFG
                     IIENMSYFICPCCGERTEIFGHGGANEAADELGLDFLGEVPLHSQIRAGGDSGTPITV
                     SDSDSPQAEAFRRIAERVAAKLSVLQWSQA"
     gene            2528..>4310
                     /locus_tag="VIM45_02265"
     CDS             2528..>4310
                     /locus_tag="VIM45_02265"
                     /inference="COORDINATES: protein motif:HMM:NF014045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEY8820141.1"
                     /translation="MPDEQKTPRSSPVRGRRGAAPRTSAIDKTARRATIKTDSDEPVQ
                     VESFVPAARADASPEAPPAARSRRTGQRPPTSEPEAAGASEATGIPSDVAPATPKGRS
                     GRRGKTAHTEPPVVAPVETSGPDLEFGVADASAEDLRAIARATGAIGANVAPRRSDES
                     PARAFGDDEEMVTWSPSPRVAPAGVEDDVAPGSDELSETGEESGGVRRRRRRGRGRRG
                     RGGQSGTAIDGVSENGGAATQPDTAAAPSQLTPDRQLVVPRQPILPDYPVEFEPMPPP
                     TKRRPARRPGMITDPRFSREEQAEIDQFVRTSPSVAAPVVSGTDEFRAALLTADEKRE
                     LEQAAPRPRSRRGRGSGRAAASPSPQETEPGLQATPAFAPMPVAPAARAGQNPLEALI
                     ARQNVILDTLMERQISLLRNIERSMIALDQRLGNGGQAAAKAARVGVFVDVPNVIYAA
                     ERIGCTIDFGKLLAILTRGRDLVRASAYSPVSDDPQMRVETQKFVQPFLGRGFRVVTK
                     PLKRFADGTMKGNFDVELAMDVLTMADRLDVVCLVSGDGDFSRLVEMIAVKGVRVEVI
                     AFSSSTSSELRAACDEYIDLGTRLREIC"
BASE COUNT          806 a         1365 c         1376 g          763 t
ORIGIN      
        1 ccacgccagc gactgccgga gtcactggaa tcattctccc agatagagtc gctcgacgaa
       61 ttcgagaact cgcggatctc gccacaaact ttgcatttcc cgacgctcgt cgagccgttg
      121 ggcgtctcga tacgccagtg gtgccggcac accggcagct cgggcgctcc ctctacttcc
      181 tcagagaccg gctgttccat gatgttcgtc tgctgcatca tatcctcccg tccgcacaag
      241 ccgcggacca aaactctcgc gttgtacgtg tcgtcaccta ggaacagtta aacctagcat
      301 gaatagtggc cggctgtcaa ggattctacg gtatttacag tatagacttg gttatcgttg
      361 acaagtatta ttgcgcgttt ctacaattga agtcggtaga ttttccgggc gggttctcgc
      421 ctcaatgcgg gcctgtccac ggggtccccg tccatgcatt cctcaaaaag ccagatactg
      481 gtcgcgctca agcgacaagg tcgatgcagc gttgacgaac tagcgattgc gttgcacttg
      541 gcgccgatga cggtgcgcca gcatctggcg acgctggaac gggatggcct cgtcgtttct
      601 gttgaagaac gccagcgaat gggccgtccc catttcgtgt tctcattgac cgagcagggc
      661 gacgaaagct tcggtatgcg gtatgaccgc atcgcgctcc aactgctact cgaggtgggc
      721 cggctcgaca gccaggagat cgccggattg tcggcggaag aaaagacggc gctccttttc
      781 gacaggctgg ccgaccgatt tatcagccgc taccgcccgc gtatgggccc gctcaccctg
      841 ccgcaacgca tcgccgcggt atcagacgcg ttacacaccg aaagcggctt caccgaatgg
      901 gccgcgaccc ccgagggtta cgaaatccgc gattacaact gctttttcga caggctgaac
      961 ggtacgaccg ccaatcattg tcgttggcat gagcgcattt tgaccgagtt gctcgagacg
     1021 ccggtcgaat gtcgaagcga cgcgccgcca tccgtccaac tgtgccactt tgtcatcaag
     1081 agtcccaaca cgcgagccgg tgcccgcccc atggcggcgg tgctgtcgat ggccggcgca
     1141 caccgcgagc catccgcgac cgggtaagga gaaccatcgt gacccaagtt tcatccatcc
     1201 aagagggcgg cgccatcaag gacgccgtgc tcgcggctct cagcaccgtt caggaccctg
     1261 aactccaccg tgacctcgtt tcgctcggca tgatcgagga tctcgccgtg gacggcggca
     1321 gcgtttcatt caaagtcgtc ctgacaacgc ccgcctgtcc tgtgcgcggc gaaatcgaac
     1381 agcaggtcga acaggccgta atggctgtac cgggtgtgcg gagcgtccac gtcgtgatgg
     1441 gagcgcaggt ccgatcgacc cctatcaagc cgggcggacc gaagccgatc gaaggggtgc
     1501 gcaacgtcat tgccgtcgca agcaacaaag gcggcgtggg caagagcacc gttagcgtga
     1561 acctcgccgt cgcccttgcg caggcggggg cgcgtgtcgg attggccgac gccgatatta
     1621 ctgggccaaa catccctacg atgatggggt ttgaggccgg ctttcaatcg cagggcaatc
     1681 agggtctccg agcagtcgag aaatacggcg tgaaagtcgc atcgctgggc ttcctcctgc
     1741 caaaagggat gcctgttgtc tggcgcggcc cgatgatcgg tacggccgtt cgccagctac
     1801 tgcacgacat cccatggggc gaaggcggag accttgacta tctcataatc gatctgccgc
     1861 cgggcacaag cgacgcctcg atgagcatgg cacaggatgc ggcgatatcc ggcgccgtga
     1921 ttgtgagcac gccgcaggac gtcgccctcg aagacgcact gaaggccgtc ggcatgtttg
     1981 aaaagcttca cgttcccgta ttcggtatca tcgagaatat gagctacttc atctgcccat
     2041 gttgcggtga acggacggag atttttggac acggcggcgc caatgaagcc gcggacgagc
     2101 tcggcctgga cttcctggga gaagttccct tgcattcgca gatccgcgcc ggcggcgaca
     2161 gtggtacgcc tataacggtc tctgattcgg actcgcctca ggctgaagcc ttcaggcgga
     2221 tcgccgagcg cgtcgccgcg aagctcagtg tgctgcagtg gagccaggcg tgaccagcga
     2281 atcgacacct tctccgagac gccgtcgtcc tgcccccaag cgggctgctg aggcaaagac
     2341 cggagcggct gcgttagatg caagcggcgc cagcaagcgt gcgggaggtc cgccggctcc
     2401 ggtgtcggtc tcgggaatcc cacgcagcat attttcgtcg ccggtcctca acctcccgcc
     2461 ggcaacggca gcaccgagtg ctacgagctc taaggccgcc gagaaacctg ctgggagccc
     2521 ggcagacgtg cccgatgaac agaagacccc tcgatccagt ccggtacgcg gccgtcgcgg
     2581 cgccgcaccg cgtacatccg ccatcgacaa gacggctcgg agggcgacga tcaagaccga
     2641 ctctgatgag ccggtgcagg tcgagtcatt cgtcccggcg gctcgtgcgg acgccagtcc
     2701 cgaagctccg ccggcggccc gttcacgccg cacgggacag cggcctccga cttccgaacc
     2761 ggaggccgct ggcgcatccg aggcgaccgg gattccctcc gacgtcgcgc ctgcgacgcc
     2821 aaaaggtcgc tcggggcggc gcggcaagac ggcacacacc gagccgccgg tcgtggcacc
     2881 ggtagaaacc tctggacccg atttggaatt tggcgttgcg gacgccagcg cagaggatct
     2941 gcgggccatc gctcgcgcta cgggcgcaat cggcgccaat gtcgctccgc gacgatcaga
     3001 cgaatccccc gctcgggcct tcggcgacga cgaggagatg gtgacgtggt cgccctcgcc
     3061 gagagtagct cctgccggtg tggaggacga tgtcgcgcct ggcagtgacg agctttctga
     3121 aacaggcgag gagtctggcg gcgttcggcg ccggagacgg cgcggtcgcg gccggcgcgg
     3181 gcgcggcggc cagagcggaa cggcgatcga cggggtatcc gagaacggcg gtgcggcaac
     3241 gcagcccgat accgccgcgg cgccttctca gctcacgcca gaccgacaac tggtcgtgcc
     3301 acggcagcca atcctgccgg attacccggt cgagttcgaa ccgatgccgc cgcctaccaa
     3361 gcgccgtccc gcccgccgtc cgggcatgat caccgaccca cgtttctctc gggaagagca
     3421 ggccgaaatc gaccaatttg tacggacgag tccgtccgtc gcggcccccg tcgtttccgg
     3481 aacggacgag ttccgcgcgg cgctgctgac agcagacgag aagcgggagc tcgaacaggc
     3541 tgcgccccgg ccccgatcgc gccgcggtcg cgggagcgga cgcgcggctg cttcgccgtc
     3601 cccgcaggag accgagcctg gtttgcaggc gacgcccgcg ttcgcgccga tgccggtggc
     3661 gccggccgcc cgtgccggcc agaacccatt agaagctttg atagcccgac agaacgtcat
     3721 cctggatacg ctgatggagc ggcagatctc cctgttgcgc aatatcgagc ggtcgatgat
     3781 cgccctcgat cagcggcttg gcaacggcgg tcaggccgcg gcaaaagcgg cgcgtgtcgg
     3841 cgtattcgtc gatgttccta acgtgatata cgcggcggag cggatcggct gtacgatcga
     3901 ttttggcaag ctactcgcga ttctgacccg tggccgcgac ctggtacgcg ccagcgccta
     3961 ttcgccggtt agcgacgatc cgcagatgcg ggtcgagaca cagaagtttg tgcagccgtt
     4021 tcttgggcgt ggattccgcg tcgtcacaaa gccgctcaag cggttcgcag acggaacgat
     4081 gaagggcaat ttcgacgtcg aactggcgat ggacgtgctg accatggccg atcgcctgga
     4141 cgtggtctgc ctggtatcgg gcgacggcga cttcagccgg ctggtggaga tgatcgccgt
     4201 caagggcgtg cgggtcgaag tcatcgcgtt ctcgtcaagc acgtccagcg aattgcgcgc
     4261 ggcctgcgat gaatacatcg atctggggac caggttgcgc gagatctgct
//
LOCUS       DATCPE010000072         3843 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U7219
            k141_466184, whole genome shotgun sequence.
ACCESSION   DATCPE010000072 DATCPE010000000
VERSION     DATCPE010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955033
            Sequence Read Archive: SRR1586279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 16:49:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 640
            CDSs (total)                      :: 631
            Genes (coding)                    :: 627
            CDSs (with protein)               :: 627
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3843
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_466184"
                     /isolate="SMAG_U7219"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..712)
                     /locus_tag="VIM45_02270"
     CDS             complement(<1..712)
                     /locus_tag="VIM45_02270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Catalyzes the synthesis of acetoacetyl coenzyme A
                     from two molecules of acetyl coenzyme A. It can also act
                     as a thiolase, catalyzing the reverse reaction and
                     generating two-carbon units from the four-carbon product
                     of fatty acid oxidation; Derived by automated
                     computational analysis using gene prediction method:
                     GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase domain-containing protein"
                     /protein_id="HEY8820142.1"
                     /translation="MAAASGQRRDAAIVGIYEYPLRKVDGITALEIKAESARNALDDA
                     GLKWGDVDGVYDAGDGGPSGSGLGIADYFGFHPSVIDTTSVGGSSYEFQASHALRDIA
                     AGKCKVALLTYGSTAHSDARAIGTGGRVSPSPSPTGNMEDSWGMTLIANYAMVAQRHM
                     YEYGTKPQQLAEISVATRAHAVRNPQAVRAMEDLGFVGTGEVSVEDVLDSRMIADPLH
                     LLECCMVSDGGGAVVIASA"
     gene            841..1431
                     /locus_tag="VIM45_02275"
     CDS             841..1431
                     /locus_tag="VIM45_02275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YfcE family phosphodiesterase"
                     /protein_id="HEY8820143.1"
                     /translation="MRLGLVTDTHLPALHRHLGELGPECAAFFQSVGMILHAGDITAP
                     SILDWLEQFAPVIAALGNNDNFSDPRLAPVQYLEVGGWRIGMRHSLVPETRPLDAVHD
                     KHFDRPVDVMIAGHTHFERIRHGHGMVLVNSGSPILPHHKETRLGTVGLLELTPESMH
                     AEIIVLGNSPGRPNPGRAHSWDTTRAVLSAHAHGHS"
     gene            1564..1749
                     /locus_tag="VIM45_02280"
     CDS             1564..1749
                     /locus_tag="VIM45_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8820144.1"
                     /translation="MSREREPKRLAHRAAEQSRFRATGRWMAEEIDEALDISQIVDTG
                     LAAETEPHPEAALTLQR"
     gene            1819..2715
                     /locus_tag="VIM45_02285"
     CDS             1819..2715
                     /locus_tag="VIM45_02285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8820145.1"
                     /translation="MTTIRTLPALPLFILGDELPSSTVTAHNASLHSDNKIHDDEVAK
                     KYGFAGGLVPGVTVYAYMVAPLAEALGERWLTTGAATVAFVQPLYEQQMAVSRATVLE
                     VNSGESGDAVAFDIRTENSAGERCGTGTATVTSEPTRGPVQVPDYVNVDPGSPPDPRP
                     ELRLETTSVGTVLARLVAPTTAQVARDYAQNVFGSNSLFTERSIWGPPLMHPGWLLSE
                     CNRIFTQSYSFGPWIHTRSQIRYLGPALAGRTFTFHGRLAEVYERRLHHYAVLDIFCI
                     DDHGRQVMQVQHTAIFKVHPKT"
     gene            2715..2981
                     /locus_tag="VIM45_02290"
     CDS             2715..2981
                     /locus_tag="VIM45_02290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8820146.1"
                     /translation="MPATHFNGAADFLIPDGTNVPARCDAGFRQGRWTGTISLVDSER
                     RLERGDVCQLRLHDEDLRVVITEQVASKRYAFIALVKPDPFETL"
     gene            2981..>3843
                     /locus_tag="VIM45_02295"
     CDS             2981..>3843
                     /locus_tag="VIM45_02295"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HEY8820147.1"
                     /translation="MSPTDPHCGQIPPTADILEAIEAAAVDIARRSGGELLRYFVEPF
                     DVRFKSKRDKDPVTDADEAVERFVRAEVACRFPGHGVLGEEGADADAGSAYLWVVDPL
                     DGTANFANGLPIFAVSIGILYQGEPVVAALYTTFGPDARPCVMHARVGSGLSIDERKW
                     QLPQRDLSGRARLSGVPAGFHHGFRFRLSQGMPPGETRSLGSIAVELGLAASGGLQYA
                     LFASPKIWDVAAGVLLVQEAGGSVYTDRNGRWQQLRRFEAPPGRPLREWKQAVVAGDP
                     AALGKIVPRARV"
BASE COUNT          686 a         1235 c         1197 g          725 t
ORIGIN      
        1 cggcagaggc gatcacgacc gcaccgccgc cgtctgacac catgcaacat tcgagcaggt
       61 gcagagggtc ggcgatcatg cgcgagtcga gcacgtcttc gaccgacact tcgccggtac
      121 cgacgaatcc caggtcctcc atcgcgcgga cggcctgggg attacgaacg gcgtgggcgc
      181 gcgtcgcaac cgaaatctcc gcaagctgct gcggcttggt cccgtattca tacatgtgcc
      241 gctgggcaac catcgcgtag ttggcgatca gcgtcatgcc ccaggaatct tccatatttc
      301 ccgtcgggct gggcgatggg ctcacgcggc cgcctgtgcc gattgcgcgt gcgtcactgt
      361 gcgccgtcga gccgtaggtg agaagcgcga ccttgcactt gccggcggcg atgtcgcgca
      421 gcgcgtgcga cgcctggaat tcgtaggagc tgccgcctac cgacgtagtg tcaatcacag
      481 atgggtggaa tccgaagtag tcggcgatgc ccaggccgct gccgctgggc ccgccgtcgc
      541 cggcgtcgta gaccccgtca acatcgcccc acttcagccc ggcatcgtcc agagcgttgc
      601 gggcgctctc tgccttgatc tccaacgcgg taataccgtc gaccttccgc agcgggtact
      661 cgtagatacc cacaattgcc gcgtcacggc gctgacctga tgctgccgcc attgcttgct
      721 ccttggccca actgcgcgaa ccgcggctct atgctaccgt cactagcacg cgggcgaaac
      781 cggcatcctg tatatttttc ggtttcccca cgccgcaatc gcggattcgg gcagccccac
      841 atgcgcctcg gccttgtcac cgacacacac ctgcccgcgc tccaccggca cctcggcgaa
      901 ctgggaccgg agtgcgccgc tttctttcag tcggtgggca tgatcctcca cgcgggcgac
      961 attaccgcgc ccagtattct cgactggctg gaacagttcg cgccggtcat cgccgcgctg
     1021 ggaaacaacg acaactttag cgatccacgc ctggcgccgg tccaatacct tgaggtcggg
     1081 ggctggcgaa tcggtatgcg gcacagcctc gtaccggaga cacgacccct tgatgccgtt
     1141 catgacaagc acttcgaccg gcctgtcgat gtcatgatcg ccggccacac gcacttcgag
     1201 cgcatccgtc acggccacgg gatggtcctg gtgaactccg gcagtcccat cttgccccac
     1261 cacaaagaga cgcgactggg caccgtcggt ctgcttgaac tgacgcctga atcgatgcat
     1321 gcagagatca tcgtgctcgg caattcgccg ggccgtccga atccgggacg tgcccatagc
     1381 tgggacacca cccgcgcggt gctctcggct cacgcccacg gtcattcata gaaccggtcg
     1441 aacaagagtt gacagctcac aacaattttt gtgcttgatt actgttttgc tattaactta
     1501 tgtcccgatt tgccgttatt actatcaatt gacagaacat cagtgcggct agggggcgga
     1561 atcgtgtcac gggaacggga gccgaaacgg ctggcgcatc gggcggctga acagagccgg
     1621 tttcgggcga ccgggcgctg gatggcggag gagatcgacg aggctctgga tatatcacag
     1681 atcgtcgata ccgggctggc ggcggaaacc gagccgcacc cagaggctgc tctgacactc
     1741 cagcgctgat tgtatgtggc tgtccggcgt acactgacaa cggcgccgct gggtgcacat
     1801 tcagactgga gcgccgtgat gacgacgatc cgtacactac cggctctgcc gcttttcatc
     1861 ctcggcgacg agttgccgtc ctcaaccgtc accgcccata acgcgtcgct tcactccgat
     1921 aacaagatcc acgacgacga ggtcgcaaag aaatacggct ttgccggcgg cctcgtcccc
     1981 ggcgtcaccg tttacgcgta catggttgca ccgttggccg aagctctcgg cgaacgctgg
     2041 ctgacgaccg gcgccgccac ggtggccttc gtgcagccgc tctacgagca gcaaatggcg
     2101 gtgtcccgtg cgacggtgct ggaagtgaac agcggcgagt cgggtgatgc ggtcgctttt
     2161 gacatccgca ccgaaaattc ggccggcgaa cgctgcggga ccggtaccgc gacggtaacc
     2221 agcgagccta cgcggggccc cgtccaagtt cccgattatg tcaatgtcga cccgggatcg
     2281 ccgccggatc cgcggccgga actgcgcctc gaaacgacat cggtcggtac ggtgctcgcg
     2341 agactcgtgg cgccgacgac ggcgcaggtc gcccgcgact acgcgcagaa cgttttcggc
     2401 agcaatagtc tgttcacaga gagatcgatc tggggcccgc cgctgatgca tcctggctgg
     2461 ctgttgagcg agtgcaatcg catcttcacg caaagctaca gtttcggccc gtggatccac
     2521 acgcgttcgc agatccgcta tctcggtccg gcgctcgccg gtcgcacgtt caccttccat
     2581 gggcggctcg ccgaggtcta cgagcgccgg ctgcatcact acgccgttct tgatattttc
     2641 tgtatcgacg accatggtcg gcaggtaatg caggtacaac acacggcgat cttcaaggtt
     2701 caccccaaaa cctgatgccg gcaacgcatt tcaatggggc ggctgacttc ctgattccgg
     2761 acggcacgaa tgtgccagcc agatgtgacg ccggctttcg ccagggacgc tggacaggaa
     2821 cgatcagcct ggtcgattcg gaacgccgtc tcgagcgcgg cgatgtctgc cagttgcggc
     2881 tgcacgacga ggacctccga gtggtcatca cggagcaggt ggcgagcaag cggtatgcct
     2941 ttatcgcgct cgttaagccc gacccgttcg agacgctttg atgagtccga ccgacccgca
     3001 ttgcggtcag atcccgccaa cggccgacat cctggaggcg atcgaagcgg cggcggtcga
     3061 cattgcgcgc aggtccggcg gcgaactcct ccgatatttc gtcgaaccgt ttgacgtccg
     3121 cttcaaatcg aagcgggaca aagacccggt gaccgatgcc gacgaggcgg tcgagcgttt
     3181 cgtccgggcg gaggtggcgt gccgatttcc gggccacggc gtgctcggcg aagaaggcgc
     3241 cgacgcggat gccggctccg cgtatctgtg ggtcgtggac ccgcttgacg gaacggctaa
     3301 cttcgccaat ggactgccga tcttcgccgt ctcaatcggc attctctatc agggtgagcc
     3361 ggtcgtggct gcgttatata cgaccttcgg ccccgatgca cgaccgtgcg tgatgcacgc
     3421 tcgcgttgga agcggattga gcattgacga acgcaagtgg cagctcccgc agcgcgacct
     3481 ttctggccgc gcccggcttt ctggcgtgcc ggcggggttt caccatggat ttcgttttcg
     3541 gctttcccag ggtatgccgc ccggcgagac gcgctcgctc ggcagcatcg ccgtggaact
     3601 ggggctcgcc gcctcaggcg gtttgcaata cgcgctgttc gcttcgccga agatttggga
     3661 cgtggcggcc ggcgtactgc tggtgcaaga agccggcggc tcggtataca cagaccgaaa
     3721 cggacgctgg cagcaactgc ggcgcttcga agcgccgccg ggccggccgt tgcgtgagtg
     3781 gaaacaggca gtggtcgccg gagatcctgc cgctctcggc aaaatcgttc cgcgggcgcg
     3841 cgt
//
LOCUS       DATCPE010000073         4645 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U7219
            k141_337001, whole genome shotgun sequence.
ACCESSION   DATCPE010000073 DATCPE010000000
VERSION     DATCPE010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955033
            Sequence Read Archive: SRR1586279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 4645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 16:49:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 640
            CDSs (total)                      :: 631
            Genes (coding)                    :: 627
            CDSs (with protein)               :: 627
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4645
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_337001"
                     /isolate="SMAG_U7219"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..490
                     /locus_tag="VIM45_02300"
     CDS             <1..490
                     /locus_tag="VIM45_02300"
                     /inference="COORDINATES: protein motif:HMM:NF015922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HEY8820148.1"
                     /translation="GMEIAARGQHLFTISERGFGKRTDFAQYPRHHRGGSGVSNLKLT
                     DKTGRVAAVRAVNGTRELMIISVDGIVLRTTVDAISIIGRSTQGVTIMKMRPTDTVAA
                     IAGIESEEDDDEASSRKRKGVADGAAEATGNGHGAVAELAGFSDVVQKTPKRGRNKDG
                     AS"
     gene            complement(575..>4645)
                     /gene="gltB"
                     /locus_tag="VIM45_02305"
     CDS             complement(575..>4645)
                     /gene="gltB"
                     /locus_tag="VIM45_02305"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: protein motif:HMM:NF008730.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HEY8820149.1"
                     /translation="LSPLDYTRRLYLARKEMERHASEEQIEGFYLPSLSNRTLVYKGL
                     LVAHQLPTFYRDLRDPQFETALAVFHQRYSTNTFPNWYLSQPFRMLAHNGEINTLNGN
                     VNWTRARESGLESEVWGDRVKELVPLVQSGGSDSSSLDNVLEAIIMSGRDIRHAFMML
                     IPEAWENMPNMPKDRRAFYEYHACLSEPWDGPASMAFTDGVFVGATLDRNGLRPARYQ
                     LTSDGTIVMGSEAGMVALDPATIVEKGRLGPGQMIGVDTDKGELLYNDDIKNLVSGRK
                     TYQTWVKRQLLHLDSWLANRTSEVLPANALDVARQQIVFGYTAEELEFILKPMAAEGK
                     EPIGSMGDDTALAIFASRPRLTYAYFKQKFAQVTNPPIDPIREELVMSLDSYLGRRRS
                     FLEETEQHARLVHLASPLMIDEEIEALRKIAEPALHAITIPTLFDVGTGPEGMHRALQ
                     TLCQQASMAVDDGATILVLSDRGVSVSLAPIPMLLAIGAVHHQLIREGKRMLASIIAE
                     TGEARDVHQIALLIGFGASAVNPYLAFATLRSLVDEGNASAKDALKAVSQSKATENYE
                     KAVDAALLKIISKMGISTISGYQAAQIFEAIGLDQETLDLCFSGMTSRIGGIGICHLA
                     QETIDRHAAAFGNGRAPALDAGAYYRYRRDGEYHAFNPEVWRNLHKAVESGDYADYRQ
                     YADIVQSRNPIALRDLLEFVPTDPIPLEEVEPIEKIATRFVTAAMSLGALSPEAHATM
                     ATAMNRIGAKSNTGEGGEDSSRRVVQPNGDTSNSKIKQVASARFGVTPVYLASADELE
                     IKMAQGSKPGEGGQLPAHKVSAQIAALRHTLPGTPLISPPPHHDIYSIEDLAQLIYDL
                     KMVNPRARVAVKLVAEEGVGTIAAGVAKGYADVVHISGHDGGTGASPLSSIKSAGAPF
                     ELGLSEVQQVLVMNDLRGRVKIRADGGMKTARDVVIAAMLGAEEFGFGTAAVVAVGCK
                     MARQCHLNTCPTGVATQREDLREKFTGTPEKVVNFFMHVSEEVREILAGLGIRSLDEV
                     IGRVDLLQQVRGGEDPRVHRVDLGRILKQPDPTGQRPHRRMQERNQRAGDQPLDDKIL
                     ADLGDTIETKKPIRLSYDVTNQNRTIGARVAGQIAYRCGDEGLPANTIELQFTGSAGQ
                     SFGAFTFRGMQLVLTGEANDYVAKGMGGGEVVIRPPAGAGYTSHENTIVGNTVLYGAT
                     SGKLFVAGRAGERFAVRNSGALAVVEGVGDHGCEYMTEGVVVILGETGRNFGAGMSNG
                     VAYVLDESRDFPKKINPELVGLAQVTAAADIEIMETLIRRHFELTGSKRAQQILENFD
                     GYLPLFWKAAPHFALTEDGPMTIVNRHLRSLKDGSH"
BASE COUNT          820 a         1462 c         1437 g          926 t
ORIGIN      
        1 tggtatggag atcgcggcca gggggcagca cctgttcacc atcagcgagc gtggttttgg
       61 caagcgtacc gatttcgcgc agtacccgag gcaccaccgc ggtggatcgg gcgtctccaa
      121 cttgaaacta acggacaaga ctggacgcgt ggcggccgtt cgggccgtca acggcacccg
      181 cgagctgatg atcatctcgg tggacgggat tgtcctgcgc acgactgtgg atgcaatttc
      241 catcatcggg cgcagcacgc agggcgtcac gatcatgaag atgcgtccaa ctgatacggt
      301 ggcggcgatc gccggcatcg agtccgaaga ggatgacgac gaggcgtcgt cgcggaagcg
      361 caagggggtt gcagacggtg cagcggaagc caccggcaat ggccacggcg cagtggccga
      421 gctagccgga ttctccgacg tcgtgcagaa gacgccgaag cgcggtcgaa acaaggacgg
      481 cgcttcgtag accggttggc tgatcggcag atggaaaagg gcggccccgt aatggagggc
      541 cgtccctatg gtttggtggc aggacgcggg cccgttagtg cgaaccgtcc ttcaagctcc
      601 tcaggtggcg gttgacgatc gtcattgggc cgtcctctgt cagcgcgaag tgtggcgcag
      661 ctttccagaa gagcggcaga taaccgtcga agttttccag gatctgctgt gcccgcttgc
      721 tgcccgtcaa ctcgaagtga cggcgaatca gcgtctccat gatttcgatg tcggctgccg
      781 cggtcacctg ggctagaccg acaagctcgg ggttgatctt cttggggaag tcgcgcgatt
      841 catcgagcac gtacgccacg ccgttagaca tgccggcgcc gaaattgcga cctgtctccc
      901 caagaatcac gaccacgccc tcggtcatgt attcgcagcc gtggtcgccc acgccctcga
      961 cgaccgcgag agcgccgctg tttcgtacgg caaagcgttc gcccgctcga cccgctacga
     1021 agagcttgcc gctggtggcg ccgtacagga ccgtgttccc cacaatcgta ttctcgtggc
     1081 tggtataacc tgcgccggca ggaggacgaa taacgacctc gccgccaccc attcccttgg
     1141 ccacgtagtc gttcgcctcg ccggtgagca ccagctgcat cccccggaag gtgaaggcgc
     1201 caaagctctg gccagcgctg cccgtgaact gcagctcgat cgtatttgcc ggcaacccct
     1261 catcgccgca gcgataggcg atttggccgg caacgcgcgc cccaatcgtt cggttctgat
     1321 tcgtcacgtc gtatgacagt cggatcggct tcttcgtctc gatcgtgtcg ccaaggtcgg
     1381 ccagtatctt gtcatccaac ggctgatccc cagcgcgctg gttgcgttct tgcatgcgac
     1441 ggtgcggccg ctggccggtc ggatcgggct gcttcagaat gcgcccgagg tccaccctgt
     1501 gtacgcgtgg atcctcgccg ccgcggactt gctgcaacag gtcaacccgc ccgatgacct
     1561 cgtcgagcga ccgtattccg agcccggcaa ggatctcgcg aacttcctcg gagacgtgca
     1621 tgaagaagtt gacgaccttt tcgggagtgc ccgtgaactt ctcacgcaga tcctctcgct
     1681 gcgtcgcgac gccggttggg cacgtgttta gatggcactg tcgcgccatc ttgcaaccca
     1741 cggcgacgac tgccgcggtc ccgaagccga actcctcagc gcccaacatc gccgcaatga
     1801 cgacgtcgcg ggcggttttc atgccgccgt ccgcacggat cttcaccctg ccgcgcaggt
     1861 cgttcatcac gagcacttgc tggacttcgg agagtccgag ctcgaacggc gcgccggcgc
     1921 tcttgatcga agagagcggg gacgcccccg tgccgccgtc gtgtcctgag atgtggacca
     1981 cgtcggcgta acccttcgcc acgcctgcgg cgatcgtgcc cacgccttcc tctgcaacca
     2041 gcttcacggc tacccgcgcc cgcgggttca ccattttaag gtcgtagatc agctgcgcca
     2101 ggtcctcgat actgtagatg tcgtggtgag gcggcggcga tattagcggt gtgccgggca
     2161 atgtatgccg tagagcggcg atctgtgccg acaccttgtg cgccggaagc tgaccgccct
     2221 cgccgggctt actgccctgg gccatcttga tctcaagctc gtctgccgag gccaggtaga
     2281 ccggcgtcac gccgaagcgg gctgaggcca cctgcttgat cttgctgttc gaagtgtccc
     2341 cgttcggttg aacaacccgc cgcgacgaat cctcgccgcc ctcgcccgtg ttgctcttgg
     2401 cgccgatgcg attcatggcg gtcgccatcg tcgcgtgcgc ttccggtgaa agcgctccga
     2461 gcgacatcgc ggcggtcacg aagcgcgtgg cgatcttttc tatcggctcg acctcttcca
     2521 acgggatcgg gtcggttggg acgaactcca acagatcgcg caacgcgatc gggttgcgcg
     2581 actgcacaat gtccgcgtac tggcggtagt ccgcatagtc gccggactcc acggccttgt
     2641 gcagattgcg ccatacttcc gggttgaagg cgtggtattc gccgtcgcgg cggtagcggt
     2701 agtacgcgcc cgcgtcgagc gcgggcgccc tgccgttgcc gaatgccgcg gcatgacggt
     2761 cgatggtttc ttgcgctagg tggcagatac cgatgccgcc gatgcgcgaa gtcatgccgc
     2821 tgaaacagag gtcgagcgtt tcctgatcga ggcctatcgc ctcgaagatc tgcgcagcct
     2881 gatagccgct gatcgtcgaa atccccatct tggagataat cttcagcagc gcggcgtcaa
     2941 ccgctttttc atagttctcg gtggccttcg actgcgatac cgccttgagc gcatctttgg
     3001 ccgacgcgtt gccttcgtcc acgagcgaac gcaacgtcgc aaacgcgagg tacgggttga
     3061 cggcgctggc gccgaaaccg atcaacagcg caatctggtg aacgtcccgc gcttcgccgg
     3121 tttcggcgat tatgctcgca agcatccgct taccttcgcg gatcagctga tggtgcacgg
     3181 ctccgatcgc gagcagcatt ggaattggcg ccagcgaaac cgagacaccg cgatcgctga
     3241 gcaccaggat cgttgcgccg tcgtcgaccg ccatcgacgc ctgctgacac agtgtttgca
     3301 gtgcccgatg catgccttcc gggcctgttc cgacgtcaaa tagcgtcgga atcgtgatcg
     3361 cgtgcagggc cggctcggcg atcttgcgca gcgcctcgat ctcttcgtcg atcattaacg
     3421 ggctggcaag gtgtaccagc cgggcgtgct gctcggtctc ttcgaggaag ctgcgccgcc
     3481 tgcccaggta gctgtcaagt gacatcacca gctcttcgcg gatcgggtcg atcggcgggt
     3541 tggtgacctg ggcaaacttc tgcttgaaat aggcgtaggt aagccgtggc cgcgaagcga
     3601 aaatcgccag agccgtgtcg tctcccatcg agccgatcgg ctccttcccc tcggcggcca
     3661 tcggcttcaa aatgaattcc agctcttcgg ccgtatagcc aaacacgatc tgctgccgcg
     3721 cgacatccaa cgcatttgcc ggcaacacct cggaggtacg gttggccagc cacgagtcga
     3781 ggtgcagcaa ctgccgcttc acccaggttt ggtacgtttt tctgcccgaa accaagttct
     3841 ttatgtcatc gttgtaaagc agctcgccct tatcggtgtc cacgccaatc atctgaccag
     3901 gtcccagcct gcccttctcg acaatagtcg ccgggtcgag cgcgaccatg ccggcctcgg
     3961 agcccatgac gatcgttccg tccgatgtga gctggtaacg cgccgggcgc aggccgttgc
     4021 ggtccaaggt tgcaccgacg aatacgccgt cggtaaaagc catgctcgcc ggcccatccc
     4081 atggctcgct gaggcaggcg tggtactcgt aaaacgcacg cctatctttc ggcatgttgg
     4141 gcatgttctc ccacgcctct gggatgagca tcatgaaagc gtggcggata tcccggccgg
     4201 acattatgat cgcctcgagc acgttgtcga ggctcgacga gtcactgccg cccgattgca
     4261 ccagcggcac cagctccttg acgcggtcgc cccagacctc ggattccagg ccggactcgc
     4321 gggctctcgt ccaattcacg ttgccgttca gcgtattgat ctcgccgttg tgggccagca
     4381 tgcggaatgg ttgtgatagg taccagttcg gaaaggtgtt ggtgctgtag cgctggtgga
     4441 aaacggcaag tgcggtttcg aactgcgggt cgcgcaagtc gcgatagaac gtgggcagtt
     4501 ggtgcgctac caggagtccc ttatagacca gcgtgcggtt ggagagcgaa ggcagataaa
     4561 agccttcaat ctgctcctcc gacgcgtgcc gctccatctc cttgcgcgca aggtagagcc
     4621 ggcgtgtgta atcgagcggc gacaa
//
LOCUS       DATCPE010000074         3147 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U7219
            k141_596600, whole genome shotgun sequence.
ACCESSION   DATCPE010000074 DATCPE010000000
VERSION     DATCPE010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955033
            Sequence Read Archive: SRR1586279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 16:49:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 640
            CDSs (total)                      :: 631
            Genes (coding)                    :: 627
            CDSs (with protein)               :: 627
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3147
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_596600"
                     /isolate="SMAG_U7219"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1255)
                     /gene="dnaG"
                     /locus_tag="VIM45_02310"
     CDS             complement(<1..1255)
                     /gene="dnaG"
                     /locus_tag="VIM45_02310"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEY8820150.1"
                     /translation="MNDNAIETVRQRVDIVELVGEQVQLQKSGRNFKANCPFHSEKTP
                     SFYVYPERQTWHCFGACATGGDVFSFVMTRENAGFGEALRTLAERAGIVLEKTRPDES
                     SQPRDRLIAANEAAIAYFRQALQGSSAGEEARAYVARRGLDAPTVAVFELGFAPDGWD
                     NLRSYLLEKGFSEEEQLEAGLLTTGERGSPYDRFRRRLIFPIRDDRGRAVGFGGRALD
                     DAKPKYLNTPQTPLFDKGNLLFLLDRAKDAIRGAGEAVVVEGYLDAITAHQFGYANVV
                     ATLGTAITERHVQLLKRLSHKVTLCMDADAAGQDAALRGEEVARTLRLDDGAMTEAVV
                     AWDGLVRVQARSPIELRVFTVPSGKDPDEAIREDTVAWPAWVAAAMPPFEFRLRREED
                     RTNMNDPRARVELADRMLPLLLQIGD"
     gene            complement(1351..1680)
                     /locus_tag="VIM45_02315"
     CDS             complement(1351..1680)
                     /locus_tag="VIM45_02315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8820151.1"
                     /translation="MLQDFSVISGGSGVGFIQVVIGNAGDGDLVSQQIEIIGVDQTDT
                     QVLHTTTGAVTISARGSLTITTGYKPAQRTMLTVVINPNGTIREADAPPGFGDPNNAL
                     TKTVAPP"
     gene            complement(2084..>3147)
                     /locus_tag="VIM45_02320"
     CDS             complement(2084..>3147)
                     /locus_tag="VIM45_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HEY8820152.1"
                     /translation="VREMLDARERALSPFAARGATSRGRERPEPPSPLRTEYQRDRDR
                     ILHSKAFRRLKHKTQVFIAPAGDHYVTRMTHTLEVAQIARTITRALNLNEDLAEAISL
                     AHDIGHAPFGHAGEEALADLLGLSWRHNEQGARVVEKLENNGRGLNLTWEVREGIVKS
                     SKVREDIFAEAWGTASTLEGQALKIADAVAYLNHDILDAIRAGLLTEEALPADVIRVL
                     GSSHGDRINTLVSDIVNASWPATGEVPGVTEPAIAMSDEVRRVTNELREFMFENVYLL
                     EERQAEVLQAREILGHLFSYYVQHPEQLPEGFSLPDDPLRQRVTDYISGMTDRFAIRL
                     AASLGSKPARQWLDRNMEL"
BASE COUNT          534 a          971 c         1022 g          620 t
ORIGIN      
        1 ggtcgcctat ttgcagcaac agcggcagca tccggtccgc aagttcgaca cgcgcccgcg
       61 gatcgttcat gtttgtccga tcctcctcac gtcggaggcg gaactcaaag ggcggcatcg
      121 ccgccgccac ccacgccggc caagcaaccg tatcttcgcg gatcgcctcg tccgggtctt
      181 tgccggacgg caccgtgaac acccgtagct ctatgggcga ccgtgcttgt acgcgcacca
      241 gcccgtccca cgccactacg gcctcggtca tcgcgccatc gtcgagcctg agcgtccgcg
      301 ccacctcttc tccgcgcagc gcagcgtcct ggccggcggc gtcggcatcc atgcagagcg
      361 tgaccttgtg gctcagccgc ttcagtagct gcacgtgccg ctcagtgatc gcggtgccca
      421 gcgtagccac gacgttggcg tagccgaact ggtgggcggt gatagcgtcc aggtaccctt
      481 ccactacgac cgcttcgccg gcgccgcgga tcgcgtcttt cgcccgatca agcaggaaca
      541 gtaggttgcc tttgtcgaac agcggcgttt gcggcgtgtt gaggtacttt ggcttggcgt
      601 cgtcaagcgc ccggccgccg aagcccaccg cgcgtccgcg gtcgtcgcgg atcggaaaga
      661 tcaaccggcg gcggaagcga tcgtacgggc tgccgcgttc gcccgtggtc aacagacccg
      721 cttcaagttg ctcctcttcg ctgaacccct tctccagcag ataactccgc aagttgtccc
      781 agccgtcggg agcgaacccc agctcgaaca cggcgacagt cggagcgtcg agcccgcggc
      841 gcgctacgta tgcgcgagcc tcttcgccgg cggaactgcc ttgcaaagct tgccgaaagt
      901 acgcgatcgc ggcctcgttt gcggcgatca gccggtcccg tggctgtgag ctctcatcag
      961 gccgcgtctt ttccagcacg atccccgctc gctcggccaa cgtgcgaagt gcctcaccaa
     1021 atcccgcgtt ttcgcgagtc attacgaacg aaaagacatc gcctccagta gcgcaggcgc
     1081 cgaagcagtg ccatgtctgg cgctcggggt atacgtagaa cgacggtgtt ttttcgctgt
     1141 gaaaggggca gttcgccttg aagtttcggc cggacttctg tagctggacc tgctcaccga
     1201 cgagttcgac gatatcgacg cgctggcgga cggtttcgat cgcgttatcg ttcatacgtc
     1261 ccccataccg gttagaacac cgaggggcag cgaaagttac ggcctggaca gagtttctaa
     1321 taattcgcga ggcggagata gcgtctcggt ttagggtggc gcaacggtct tggttagcgc
     1381 gttgttcgga tcaccgaaac cgggtggggc atcggcctcg cggattgtcc catttggatt
     1441 gatcactacg gttagcatgg tgcgctgggc cggcttgtaa ccggttgtaa tggtcaacga
     1501 gccgcgtgcg gaaatggtca ccgcgccggt tgtcgtgtgc agcacctggg tgtctgtttg
     1561 atcgacgcca ataatttcta tctgctggct cacaagatcc ccgtctccgg cgtttccgat
     1621 caccacctgt atgaatccca cccccgaccc gccggagatg acgctgaagt cttgcagcac
     1681 aaggtccggg agcccgcggc cggcagcggg cgtcggagct ggcggagaca cgggcgttgg
     1741 gctaggagtt ggcgacggag tcagggcagg cgttggcgtg gggcttggtg ttgcgaaagg
     1801 agcagcggtt ggtgcggcga gaattgggac agaggtggga agccgcggcg cggtcgcccg
     1861 cggtgaaggt gatgcgccga ggctcgacgc ggcggtgggt gtgctcgaaa cggttcgaat
     1921 ctcgacgatg ccgcctcgct tcttgccatt cccgccaatg aatatgactg ccaacaagaa
     1981 tatgaacagg atgccggccg cggacgccat ggcccagagg attcgctgct gggcgagtgt
     2041 catatggtgg ctggcctggg agtgtgcggt tgccggtgaa ggatcacagc tccatgttcc
     2101 ggtcgagcca ctgtcgcgcc ggtttgctgc ccagcgacgc cgccagccgg atggcaaaac
     2161 ggtcggtcat gcccgagatg tagtcggtaa cacgctggcg gaggggatcg tcaggcagcg
     2221 aaaatccttc gggtagctgt tcgggatgct gtacgtagta gctgaacaaa tggccgagaa
     2281 tctcgcgcgc ctgcagaact tccgcctggc gttcttcgag caggtagacg ttttcgaaca
     2341 tgaactcgcg cagctcgttc gtcacccgcc gcacctcgtc gctcatcgca attgcgggct
     2401 cggttacgcc cggcacctcg ccggttgcag gccatgacgc gttgacgatg tcgctgacaa
     2461 gcgtattgat ccggtcgcca tgagacgaac caaggacccg gatgacgtca gccggtagcg
     2521 cttcctcggt gagaagaccc gccctaatcg cgtcgagaat gtcgtggttg agataggcga
     2581 cggcgtcggc gatcttgagc gcctggccct ccaacgtcga ggcggtgccc catgcctcag
     2641 caaagatatc ctcgcgcact tttgagcttt taacgatccc ttcccgcact tcccaggtca
     2701 ggttcaggcc ccgcccgtta ttctcgagct tttcgacgac gcgggctccc tgttcgttgt
     2761 gccgccaact caagccgagc agatcggcga gcgcctcctc gccggcgtgt ccgaacgggg
     2821 cgtggccgat gtcgtgcgcc agggagatgg cttcggcgag atcctcgttg aggttgaggg
     2881 cgcgggtaat cgtgcgcgcg atctgcgcca cttccagtgt gtgggtcatg cgggtgacgt
     2941 agtggtcgcc cgccggggcg atgaatacct gcgtcttgtg cttcagccgg cgaaaggcct
     3001 tcgaatgcaa gatccggtcg cgatcgcgtt gatattcggt tcgcaacggc gacgggggct
     3061 cggggcgctc gcgtccacgg ctggtcgctc cgcgggcggc gaacggcgac agagcacgtt
     3121 cacgcgcgtc gagcatctcg cgaaccg
//
LOCUS       DATCPE010000075         1938 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U7219
            k141_12400, whole genome shotgun sequence.
ACCESSION   DATCPE010000075 DATCPE010000000
VERSION     DATCPE010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955033
            Sequence Read Archive: SRR1586279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 16:49:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 640
            CDSs (total)                      :: 631
            Genes (coding)                    :: 627
            CDSs (with protein)               :: 627
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1938
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12400"
                     /isolate="SMAG_U7219"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="VIM45_02325"
     CDS             <1..314
                     /locus_tag="VIM45_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010937097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY8820153.1"
                     /translation="YVISIAARMVGMHQQTLRYYERVGLIEPSRTRGNIRLYSPANIN
                     RLRQIQRLISDLGVNLAGVEVIINMGHRLQSLEAEIQQLRAEVEHCHTQHQQLRGRAS
                     S"
     gene            340..>1938
                     /locus_tag="VIM45_02330"
     CDS             340..>1938
                     /locus_tag="VIM45_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010937098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HEY8820154.1"
                     /translation="MMRQDRFTEQAQEVLTASQELMRQQRHTQWDVEHVLLSLVKREG
                     GLARQVLERFRVPLDELTRTLETHLNAAPKSAFPAVQAYVTPRLVRMLENADAEANRL
                     KDEYIGVEHLLIAIGDEREGESARILRTYGVDKERIYRALQEIRGTHRVDNPRAESKY
                     QALNKYATDLTELAREGKLDPVIGRETEVKRVMQILNRRTKNNPVIIGEAGVGKTAIV
                     EGLAQKIVLGDVPENLQDRRLLALDMGALVAGSRFRGEFEERLQAVMAEVRQAAGEVV
                     LFIDELHTVVGAGAAEGSIDASNMLKPALARGELRAIGATTLDEYRQHIERDPALERR
                     FSPVYVDEPSVKETVDILKGLRPRYEQHHKVKISDEAISAAATLSDRYITERFLPDKA
                     IDLIDEAASKHVIEAESLSPELQSMKLRLNQLSRETDAAVQAEDYEDAARIKSDLLRL
                     QEDYATAKEEWQAKSPHKTEIDEADIAELIASITGVPVSRMLEGEAAKLLDMEQRLHE
                     RVIGQDAAISVLADAIRRARAGLKD"
BASE COUNT          447 a          570 c          626 g          295 t
ORIGIN      
        1 gctacgtcat tagcatcgcg gcgcgaatgg tggggatgca ccagcagacg ttgcggtact
       61 acgagcgcgt tgggttgatc gagccgtctc gcacgcgcgg aaacatcagg ctgtattcgc
      121 cggccaacat caatcgactt cggcagatac agcggctcat cagcgacctg ggcgtcaacc
      181 tggccggcgt cgaggtgatc attaacatgg gccatcggtt acagagtctc gaagcggaga
      241 tccaacaact ccgcgcagag gtcgagcatt gccacacaca acatcaacaa ttgcgcggca
      301 gggcatcgag ctgaaccagc gataggtgac aactacccca tgatgcgtca agacagattt
      361 acagagcagg cacaggaggt gctgaccgcc tcgcaggagc tgatgcgcca gcagcgccat
      421 acccaatggg atgtggagca cgtgctgcta tccctggtca aacgtgaagg cggcctcgcc
      481 cggcaggtcc tcgagcgatt tcgcgttccg cttgacgagc ttacgcggac acttgaaacg
      541 cacctgaacg cggcaccgaa gtcggcattc ccggctgtgc aagcgtacgt tacgccgaga
      601 ttggttcgaa tgctggagaa tgcagacgcc gaagcaaacc gtctcaagga cgaatacatc
      661 ggtgtcgagc acttgctgat cgcgatcggc gatgaacgcg agggcgaatc ggcccgtatt
      721 ttgcgtacct acggcgtgga caaggaacgg atctatcggg cgttgcagga gattcgcggg
      781 acccaccgcg tcgataaccc gcgcgccgag agcaagtatc aggccctcaa caagtacgcg
      841 acggacctga ccgaactggc tcgcgaaggc aagcttgacc ccgtaatcgg tcgagagacc
      901 gaggtgaagc gagtcatgca gatcctcaac cgccgcacca agaacaaccc ggtaatcatc
      961 ggcgaagcgg gcgtgggcaa gaccgcgatc gtcgaaggcc ttgcgcagaa gatcgtgtta
     1021 ggcgatgtgc ccgaaaacct ccaggatcgc cgcctactgg cattggatat gggcgccctg
     1081 gtggccgggt cacgcttccg aggtgaattc gaagaacggc tgcaggccgt gatggccgaa
     1141 gtgcgccagg cagccggaga ggtcgtgctg tttatcgacg agctccacac cgtcgtcgga
     1201 gccggtgcgg cggaagggtc gatcgacgcc agcaacatgt tgaagccggc gcttgcccgg
     1261 ggagagctgc gggcgatcgg cgcgaccacg ctagacgaat accgccaaca catcgagcgc
     1321 gacccggcgc tggagcggcg cttttccccg gtgtacgtgg atgaaccgag cgtcaaggaa
     1381 acggtcgaca tcctcaaggg gctgcggccg aggtacgaac agcaccataa ggtgaagata
     1441 tcggacgagg cgatcagtgc cgcggctacg ctgtccgacc ggtacatcac ggaacgcttc
     1501 ctgcccgaca aggcgatcga cctgatcgac gaggccgcga gcaaacacgt aatcgaagcg
     1561 gaaagcctga gcccggagct gcaatcgatg aagttgcggc tcaaccagct ttcgcgcgaa
     1621 acggacgccg ccgtgcaggc cgaggactac gaggacgcgg cacggatcaa gagcgacttg
     1681 ctgcggctcc aggaggatta cgccacggcc aaggaagagt ggcaggctaa gagtccgcac
     1741 aaaacggaaa tcgacgaagc ggacatcgcc gagctaatcg cgagtatcac cggtgtgccg
     1801 gtgagccgca tgctcgaagg cgaggcagca aaactgctcg atatggagca gcgcctccac
     1861 gagcgcgtca tcggccagga tgccgccatc agcgtcctgg ccgacgcgat ccgccgggcg
     1921 cgtgccggcc tgaaggac
//
LOCUS       DATCPE010000076         5432 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U7219
            k141_46610, whole genome shotgun sequence.
ACCESSION   DATCPE010000076 DATCPE010000000
VERSION     DATCPE010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955033
            Sequence Read Archive: SRR1586279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 5432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 16:49:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 640
            CDSs (total)                      :: 631
            Genes (coding)                    :: 627
            CDSs (with protein)               :: 627
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5432
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46610"
                     /isolate="SMAG_U7219"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..623)
                     /gene="rsmI"
                     /locus_tag="VIM45_02335"
     CDS             complement(<1..623)
                     /gene="rsmI"
                     /locus_tag="VIM45_02335"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEY8820155.1"
                     /translation="MYLVPTPIGNLEDVTLRSLRVLREAGLILAEDTRTTRRLLDRYK
                     ITGQLESYTEHSHAVRAPRVMEALATSDVALVSEAGMPGINDPGQGLVAEAVTAGYRV
                     IGLPGASAVPLAVAVAGFPITSFTYLGFLPHRSAERRKLLTANGWSGHTLVGFETPHR
                     VLASLRDLEAVLGDRRLAVCRELSKLHEEVFRGSAATAIEHFSTPKG"
     gene            complement(702..1580)
                     /gene="prmC"
                     /locus_tag="VIM45_02340"
     CDS             complement(702..1580)
                     /gene="prmC"
                     /locus_tag="VIM45_02340"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEY8820156.1"
                     /translation="MFRPGPNQPPTGTDALRRGTAALRAAGIDDPALEAELLLRHVLG
                     LDKSYFFLRLPDQLSHEQHRSFLKLLGERAAHKPAAYISGHREFYGLDFRVQPGVLIP
                     RPETEMLVEQSLALAGYLRDRGKRVRFVDVGTGSGAIAIAVAKHAPEAGVMATDRSRD
                     ALVIAAYNARQMRFAGRVKFLEGDLLDPVHGQVDIVAANLPYIPSAAIESLAPEVKDW
                     EPREALDGGADGLDAIRRLIACLPVRVRPGGAAVLEIGYDQGAAVLALTASLSGAGTT
                     IVKDLSGFDRVAVIKL"
     gene            complement(1580..2659)
                     /gene="prfA"
                     /locus_tag="VIM45_02345"
     CDS             complement(1580..2659)
                     /gene="prfA"
                     /locus_tag="VIM45_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015865328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEY8820157.1"
                     /translation="MWERLDDIERRYEELSALMARPEIASDFVRYQALAKEQSALAAT
                     VELYREYKNAGRELEQARVLVEESDDRDMRELSQEEVARLAPQVEGLTQLLKLAVLPK
                     DPRDERNVILEVRGAEGGEEAALFAADLFRMYTRYAERHGWKTEIISSTLSDIGGFKE
                     VVLEVRGNGAYSRLKYEGGGHRVQRVPETEAKGRIHTSLATVAVLPVAEEVEFRIDPK
                     DVREDVYHSGGAGGQNVNKVATAIRLTHFPSGIVVTCQDERSQLQNRVKAWNFLRAKL
                     LDLEERRQMQEVTDKRRSQVGSGERSEKIRTYNFPQDRLTDHRVPITVHNLPGILDGQ
                     IDDLIDAVIIWNQQRLFDESNAAAD"
     gene            complement(2735..4006)
                     /gene="hisS"
                     /locus_tag="VIM45_02350"
     CDS             complement(2735..4006)
                     /gene="hisS"
                     /locus_tag="VIM45_02350"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEY8820158.1"
                     /translation="MYKAPRGTVDVLPSEQPYWRYVMATAERLSALYGYQRIDTPTFE
                     EARLFIHGTGDATDIVRREMYTFEDRDHRLMALRPEGTPNTLRAYLEHGLFNLPQPVK
                     LYYLAPAFRYERPQAGRFREHRQFGLEAIGEEDPAIDAELIDFLWQFYLQLGLRGLTV
                     LLNSIGDRNCRPEYIRVLRKYYADKLDQVCSDDRDRFERNPLRLLDCKVPGCQPIVAS
                     APTIDQHLCEECAAHFSRVQRYLRDLQIPFAVTPRLVRGLDYYTRTVFEIIPENSGST
                     GTIGAGGRYDGLMEILGGKPTPGIGFATGLERIVLNLQKAGVEPPPLPGPDVYVAFKS
                     EEDRQLAVQICGQLRHQGVGALVATGNRSLKSQLRQADRTPARFALIIGGAPGETVLK
                     DLRGQVAEVAVESDQAIFMIMARLQGDGETA"
     gene            4081..>5432
                     /locus_tag="VIM45_02355"
     CDS             4081..>5432
                     /locus_tag="VIM45_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015406698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HEY8820159.1"
                     /translation="MEVAELLSRVSEYLPADRVAIIEDAYRFAESAHDGQMRLSGEPY
                     IQHPLHAAYTVAGLQLDAGAVSAALLHDTMEDCGVTPDRMQEQFGPEITRLVEGATKL
                     SKMHWHPAEGRLNDSLQAENLRKMFLAMAEDVRVVIIKLADRLHNMRTLDAQDPVKQV
                     RIAAETMDIYAPLAHRLGIWQIKWELEDLAFRHLEPEKYREIAQLVSSKRASRERYIG
                     QVEAILTEELAKSQIAAEVKGRAKHIYSIYQKISKYEVQGKSFNEIYDLLALRVLVDS
                     VTDCYNALGVVHSLWRPIPGQFDDYIASPKDSMYQSLHTTVMCLGQRPLEIQIRTHEM
                     HDMSEYGIAAHWRYKEGGKRDQRYEERMAWLRRLLEWQRDMSSADDFVDSVKTDVFSD
                     QVFVYTPKGEIKDLPAGSTPLDFAYRIHTDLGHNCVGAKVNGRLVSLNSALSNGDVIE
                     V"
BASE COUNT         1034 a         1672 c         1669 g         1057 t
ORIGIN      
        1 tcgcccttcg gcgtcgagaa atgttcgatc gccgtggcgg ccgaacctcg gaagacctct
       61 tcgtggagtt tcgatagttc gcggcagacg gcgaggcgcc ggtcgccgag cacggcttca
      121 aggtcacgca gcgatgcaag aacgcggtgc ggcgtctcga agccgaccag tgtgtggccg
      181 gaccagccgt tcgcggtaag caatttccgg cgttccgcgg aacgatgcgg cagaaagccg
      241 aggtaggtga acgacgtaat cgggaaaccg gcgacggcaa cggccagcgg caccgccgaa
      301 gcgccgggaa gtccaatcac ccggtatccc gcggttaccg cttcggccac gagtccttga
      361 cctgggtcgt tgattccggg catccccgcc tcggagacga gcgccacgtc ggacgtggcc
      421 aatgcttcca ttacgcgcgg ggcgcgcacg gcatggctgt gttcggtgta gctctcgagc
      481 tgaccggtga tcttgtagcg gtccagcagg cggcgcgtcg tacgggtatc ttccgcgagg
      541 atcaagcctg cctcgcgtag gacccgcagc gagcgcagcg tcacatcctc gaggttgcct
      601 attggcgttg gaacgagata caagacgccg atggctgctc ccggcgcaga ccgctgccgt
      661 cctagcgcgg cctgccagtc gcattgtagc gcggtggatc tttacaactt gattactgcg
      721 acccggtcaa atccggaaag gtctttgacg atggtcgtgc cggcaccgct gagcgacgcc
      781 gtaagagcga gaactgcggc cccctggtcg tagcctatct ccagcaccgc ggccccgccg
      841 gggcggacgc gcaccggcaa gcaggcgatt agacggcgaa tcgcgtccaa cccgtcggcg
      901 ccaccatcga gtgcctcgcg cggttcccag tccttcacct ctggggcgag cgattcgatc
      961 gcggcggacg gaatgtaggg caggttagcc gcgacgatat cgacttgacc gtgaaccgga
     1021 tcgagcaggt ccccttcgag aaatttgacc cggcccgcga aacgcatctg tcgggcgtta
     1081 taagccgcga taaccagggc gtcccgagat cggtccgttg ccattacccc cgcttccggt
     1141 gcatgtttcg caacggcgat tgcaatcgcg ccggagccgg ttcccacatc gacaaaccgg
     1201 acccgtttgc ctcggtcccg cagatagccg gccaacgcca ggctctgttc cacgagcatc
     1261 tctgtctcgg gccgcgggat caggactccg ggctgcacgc gaaaatcgag gccataaaac
     1321 tctcgatgcc cggatatgta ggcggccggc ttgtgggcgg cgcgctctcc caacagtttc
     1381 aggaacgacc gatgctgctc gtggctgagc tggtctggca gtctcaggaa gaagtagctc
     1441 ttatcgagcc ccaacacatg gcggagcagc agttcggctt ccagcgccgg atcgtcgatt
     1501 ccggcggcac ggagtgcggc ggttccccgt ctcagtgcat cggtacccgt cggcggctga
     1561 ttcggaccgg gcctgaacat cagtcggccg cggcgttgct ttcgtcgaat aggcgctgct
     1621 ggttccagat gataacggcg tcgatcaggt catctatctg gccatccagg attcccggca
     1681 ggttgtgaac cgtgatcggc acacggtgat cggtgaggcg atcctgcggg aagttgtagg
     1741 tgcgaatctt ctcggagcgc tcacccgagc cgacctgcga cctccgcttg tcggtcactt
     1801 cttgcatctg gcgtcgctct tccaggtcaa gaagctttgc ccgcaggaag ttccacgcct
     1861 ttacgcggtt ctggagctgc gatcgctcat cctgacaggt aacgacgata ccggaaggga
     1921 agtgggtaag gcggatggcc gtggcgacct tgttcacgtt ttggccgccc gctccgccgc
     1981 tgtgatacac gtcctcgcgc acgtccttgg ggtcgatacg aaactcgact tcctcggcca
     2041 caggcaggac ggcgaccgtc gcgagtgagg tatggatgcg gcccttggcc tcggtctcgg
     2101 gaacgcgctg gacgcggtgg cctccaccct cgtacttgag gcgagagtag gcgccgtttc
     2161 cccgcacttc gaggacgact tccttgaagc caccgatgtc gctaagcgtg ctggatatga
     2221 tctcagtttt ccagccatgg cgctcggcgt agcgcgtgta catgcgaaac aggtcggcgg
     2281 caaagagggc ggcctcttca ccgccttcag cgcctcgtac ttccaggatt acgttacgct
     2341 cgtcgcgcgg atcttttggc aaaacagcca gctttagcag ctgggtaagg ccttcgacct
     2401 ggggcgcaag gcgggccacc tcttcctggc taagctcgcg catgtcgcgg tcatcgcttt
     2461 cttcgaccag cacacgcgcc tgctcgagct cgcggcccgc gtttttgtac tcccgataca
     2521 actctacagt ggcagcaagt gcagactgct ctttggccag cgcctggtag cgcacgaaat
     2581 cagacgcgat ttcggggcgc gccattaagg cggacagctc ttcgtaccgc cgttcgatgt
     2641 cgtcgaggcg ctcccacatg aagggacttc ctactctccg ctatcgaacc tacgcttcga
     2701 ggtgagccgc atgggttcag ctataccgag tttatcacgc ggtctcgcca tcaccctgta
     2761 gcctcgccat aatcatgaag atggcctggt cggactccac cgccacttcc gcgacctggc
     2821 cgcgcaagtc tttgagcact gtttcgcctg gcgctccgcc gatgattagc gcgaacctgg
     2881 caggcgtgcg gtctgcctgg cgaagctgcg atttgaggct acggttgccc gtcgctacca
     2941 gtgcgcccac gccctggtgg cgcaactggc cgcaaatctg gacggcgagc tgtcggtctt
     3001 cttcgctctt gaacgcgacg tagacgtcgg gtcccggcaa cggcggcggc tcaacgcctg
     3061 ccttctgcag gttcagaacg attcgctcga gccccgtcgc gaaaccgatg cccggcgtcg
     3121 gcttaccgcc caggatctcc atcagtccgt cgtagcggcc cccggcgccg atcgtgccgg
     3181 tgctgcccga gttctcgggg atgatctcga ataccgtgcg cgtgtagtag tccaggccgc
     3241 gtacgagccg cggcgtcacc gcaaatggaa tctgcaggtc tcgcaagtaa cgttgaaccc
     3301 tggaaaagtg tgcggcgcac tcttcgcaga ggtgttgatc gatggtcggt gcgctcgcga
     3361 cgatcggctg gcaacccggc actttgcagt cgagcaagcg cagcggattc cgctcgaatc
     3421 gatcacgatc gtcactgcac acctggtcca gcttatcggc gtaatacttg cgcaggacgc
     3481 gaatgtattc ggggcggcaa tttcggtcgc cgatgctgtt gagcaagacg gtgagtccgc
     3541 gcaggccaag ctgaaggtag aactgccaga ggaaatcgat cagctcggca tctattgccg
     3601 ggtcttcttc gccgatcgct tcaaggccaa actgccggtg ctcgcgaaaa cgccccgcct
     3661 ggggccgttc gtatctaaac gcgggcgcga gatagtacag cttcaccggc tgcggaaggt
     3721 tgaaaagtcc gtgttccagg taggctcgga gcgtatttgg cgtaccttcg gggcgtagcg
     3781 ccatcaaccg gtggtcgcgg tcctcaaacg tgtacatttc gcgccgcacg atgtcggttg
     3841 catcgccggt tccgtgaatg aacagccggg cttcttcaaa tgtcggcgtg tcgatgcgtt
     3901 ggtagccata cagggcgctg agtcgctccg cggtcgccat aacgtaccgc cagtacggct
     3961 gctccgacgg caggacgtcg acggttcccc gaggtgcctt gtacatcatt ctctctgata
     4021 tgcttaatag taaggtcaca atcgctggat tgacccgttt gtggccgatg ccggagtcct
     4081 atggaagtcg cagaactgtt gtcgcgcgtt tcggagtatc tgccggcgga tcgcgtcgcg
     4141 ataattgaag acgcctaccg cttcgccgaa tcagcacatg atggacagat gcgactgtcc
     4201 ggcgaaccgt acattcaaca tccgttacac gcagcataca ccgtggccgg ccttcaactc
     4261 gacgccggcg ccgtttcggc ggcgctattg cacgatacga tggaagactg cggtgtcact
     4321 ccggaccgga tgcaggagca gtttggcccg gagatcacca ggctcgtgga aggggcaacc
     4381 aagctcagca agatgcattg gcacccggca gagggccgtc tgaacgacag tttgcaggcc
     4441 gaaaacctgc gcaaaatgtt cttggccatg gccgaagacg tgagggtcgt catcatcaag
     4501 ctggccgaca ggctgcacaa catgcgcacg ctggacgcac aagacccggt aaagcaggtc
     4561 cgaattgcgg ccgagacgat ggatatctac gcgccgctgg cgcaccggct cgggatatgg
     4621 cagatcaaat gggagctgga agacctcgcc ttccgtcacc tggaacccga aaagtaccgc
     4681 gagattgctc agctcgtctc atcaaaacgc gcatcccgtg aacggtacat cggccaagtc
     4741 gaggccatcc tgacggaaga gctggccaag agccaaatcg ccgccgaggt gaagggtcgc
     4801 gccaagcaca tctatagcat ctaccagaag atctcgaagt acgaggtgca aggcaagtcg
     4861 ttcaacgaga tttacgacct gcttgcactg cgcgtgctgg tggattccgt taccgattgc
     4921 tacaacgcgc tcggcgtggt tcatagcctc tggcgcccaa tcccagggca gtttgacgac
     4981 tacattgcca gtcccaagga cagcatgtat caatcgctgc acacgaccgt catgtgcctt
     5041 ggtcagcgac cgctggagat acagatccgt acacacgaga tgcacgacat gtctgagtac
     5101 ggtatcgctg cccattggcg ctacaaggaa ggcggcaaac gcgaccagcg ctacgaagag
     5161 cgaatggcgt ggctccggcg actgctcgag tggcagcgag atatgtcctc cgccgacgac
     5221 tttgtcgatt cggtaaagac ggacgtcttc tcggatcaag tattcgtcta cacgccgaaa
     5281 ggcgagatca aagacctgcc tgcgggctcg acgccgcttg acttcgccta ccgtatccac
     5341 accgacctgg gtcacaactg cgtcggcgcc aaagtcaacg gccgcctggt atcgctcaac
     5401 tccgcgctgt cgaacggcga cgtgatcgag gt
//
LOCUS       DATCPE010000077         3902 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U7219
            k141_533321, whole genome shotgun sequence.
ACCESSION   DATCPE010000077 DATCPE010000000
VERSION     DATCPE010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955033
            Sequence Read Archive: SRR1586279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 16:49:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 640
            CDSs (total)                      :: 631
            Genes (coding)                    :: 627
            CDSs (with protein)               :: 627
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3902
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_533321"
                     /isolate="SMAG_U7219"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..993
                     /locus_tag="VIM45_02360"
     CDS             <1..993
                     /locus_tag="VIM45_02360"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HEY8820160.1"
                     /translation="GYVEDEKLNIARRFLVPRQIDENGLTPANLTFNDAALRRLIREY
                     THEAGVRGLEREIGQICRKVARQVAEGQTRLVHVGPGALARFVGAQKYFWGMAEEHDE
                     IGLATGVARTDMGGDILGIEVTLMPGKGNLILTGQLGEVMRESAQAALSFARSRSAEL
                     GISLVDAEKQDIHVHVPAGGIPKDGPSAGITIATALISALTQQPVDRQVAMTGEITLR
                     GRVLPVGGIKDKVLAAHRAGIKTFVLPAKNEKDLDEVPQLVRRSLRFVFADEMRDVLR
                     VAFPGWQPSRTAGLSDPVELRTERTAVSVPSRTHGGPPVPHLRPPEVHRSPPPA"
     gene            1082..1579
                     /locus_tag="VIM45_02365"
     CDS             1082..1579
                     /locus_tag="VIM45_02365"
                     /inference="COORDINATES: protein motif:HMM:NF014704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEY8820161.1"
                     /translation="MNWLDFAIIVTILWFAITGLTVGLLREVVTLVAAFLGVVLAGRL
                     YARLADDIRIVHADTRMDQLIAFIAIFGATALAGQIAGSLLKQTASLLLLGPVDRVAG
                     LAFGLLKGLIIVELVLIAFAVFPSAMWMKTAIDTSLLAPLFLDGAPWLLHLLPGGFRS
                     AVHAV"
     gene            1673..2200
                     /locus_tag="VIM45_02370"
     CDS             1673..2200
                     /locus_tag="VIM45_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEY8820162.1"
                     /translation="MEPAVLVLNQNYDPINVCNARRAFTLLYGGKAEVLEHGLGVLRS
                     PSFAFPLPSVIRLVYMVKRPRPQMRLTRREVFMRDRYTCQYCGRQARELTLDHVFPRH
                     RGGRHTWENLVAACKGCNHRKAGRTPSEARMLLSRAPYRPRVSSYYLFYQYLDVQHEW
                     RKFIPGWDEASIGIS"
     gene            complement(2214..2873)
                     /locus_tag="VIM45_02375"
     CDS             complement(2214..2873)
                     /locus_tag="VIM45_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEY8820163.1"
                     /translation="MYIIVVGGGKVGFYLARELVSEGHELLVIEQDPSKCERITDELG
                     DITLCGDGCEAAVLDNAGTGRANMFIAVTGGDEDNLVACQVARTKFDVPRIIARINNP
                     KNEAIFKKLGIDTTVSATAVILAHIEQALPTHHLIPLLTLRNSGLELVEAQLVQDSAS
                     VGRRVRDLLLPQRSLIPLLVDSDGFAKVPTPDTVLQDGDQVLAVTPPETEDALRAVLL
                     G"
     gene            complement(2892..3293)
                     /locus_tag="VIM45_02380"
     CDS             complement(2892..3293)
                     /locus_tag="VIM45_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEY8820164.1"
                     /translation="MKVVIMGCGRVGAQLATTLDREGHDVTILDLRSESFSKRLPSDF
                     SGRALVGNGIDMDTLRRAGIQDADAFVAVTQGDNRNVMAAQIAQHLFNVKRVVCRIYD
                     PIREELYHTLGLETISPTKVGAQLLHDALER"
     gene            complement(3339..>3902)
                     /locus_tag="VIM45_02385"
     CDS             complement(3339..>3902)
                     /locus_tag="VIM45_02385"
                     /inference="COORDINATES: protein motif:HMM:NF037043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEY8820165.1"
                     /translation="GIRDEALPSGCEVEFIQERGDLKQAVLWLGPLATTRRRATLLPG
                     GQTLTTVSVAHVPAAPPRQFLFEPGPAVIRAHAIEQVAHSLGATKIDDSTAFLCADVS
                     QQTPFATAYRVEEWMPFNLKQLRRRLRALDIGHVVVKTRASPLDPQELERSLRLEGSG
                     ERILVLTRVLGRHSVLICRVDRDQTDT"
BASE COUNT          699 a         1182 c         1262 g          759 t
ORIGIN      
        1 ggctacgtcg aagacgagaa actcaacatc gcgcggcgtt ttttggtacc acgccagatc
       61 gacgaaaacg gtctgacacc ggcgaacctg actttcaacg acgcggcgct gcgccggctg
      121 atacgcgagt acacgcacga agccggcgtg cggggactgg agcgcgaaat cggtcagatc
      181 tgtcgcaagg tcgcgcggca ggttgccgaa ggccagacgc gcttggtgca cgtcggcccg
      241 ggtgccctcg cccggttcgt cggtgcgcaa aaatacttct ggggcatggc cgaggagcac
      301 gatgagatcg gcctcgcgac aggcgtcgcc cgcaccgata tgggcggcga tattctgggt
      361 atcgaggtga ccttgatgcc cggaaaaggc aacctgatcc ttaccggaca gttgggcgag
      421 gtaatgcgag agtcggcgca ggcggcgctc tcattcgcgc gatcgcgatc ggcggagctc
      481 ggcatttccc ttgtagacgc cgaaaaacag gatatccatg tccacgttcc ggccggcgga
      541 atccccaagg acggtccttc ggctggtatc accatcgcga cggcgttgat ctcggcgctg
      601 acacagcagc cggtcgatcg acaagtggcg atgaccggag aaatcacgct tcgcggccgt
      661 gtgttgccgg tgggcggaat caaggacaag gtgctggcgg cgcaccgggc agggatcaag
      721 acgttcgtgt tgccagccaa gaacgaaaaa gacctggacg aagtgccgca gctcgtacgc
      781 cgcagtttga gattcgtatt cgccgatgag atgcgtgacg tgcttcgagt ggcgttcccg
      841 ggctggcagc cgagcaggac ggctggtttg agtgatccgg tggagctgcg aacagaacgc
      901 accgcggtga gcgtcccgag ccgaacccac ggcggaccgc cggtgccgca tctgcgtcca
      961 ccggaggtac atcgtagccc gccgccggcg tgagcagcgg agcaatcatt tcttaaaatg
     1021 ctggcgggcg gcacagtttt ttgcatatca atacggcggg cggccgggta cgggctcagc
     1081 gttgaactgg ctcgatttcg cgatcatcgt gacgatcctg tggttcgcga tcacagggct
     1141 gaccgtcggt cttctgcgcg aagtggtgac gctggttgct gcctttcttg gggtggtcct
     1201 ggccgggcgg ctgtatgcgc gtctcgcgga cgatatccgc atcgttcacg ccgacacgcg
     1261 gatggaccag ttgatcgcct tcatcgctat ttttggggcg actgcgcttg ccggccagat
     1321 cgccggcagc ctgctcaagc agaccgcgtc actgctgctg ctggggcccg ttgaccgcgt
     1381 ggccggcctg gcgtttgggc tcctcaaagg gctgatcatc gtcgagctgg tcttgatcgc
     1441 ctttgctgtg ttcccatcag cgatgtggat gaagacggcg atcgatactt cgttgctggc
     1501 accgctgttt ctcgatggcg cgccctggct gcttcacctg ctgccgggcg ggttccgttc
     1561 agccgtacac gcggtgtagt ggagacacac ccgcgcgttg tcagccgttc gaatgctctg
     1621 ttatgattcg gccaccacca cctgtgcgtg atttctcggt cgagcgtgct ctatggagcc
     1681 tgctgtactc gttcttaatc agaattacga tccgatcaac gtgtgtaacg ctcgccgtgc
     1741 cttcacgctc ctgtacgggg gcaaggccga agtgctggaa cacggccttg gcgttcttcg
     1801 cagccctagc tttgcgtttc cgcttccttc cgtcatccgc ctggtctaca tggtgaagcg
     1861 gccccgtccg caaatgcgcc tgaccaggcg cgaagtcttc atgcgcgacc gctacacctg
     1921 ccagtattgc ggccgccagg cgcgcgagtt gacgctcgat cacgtctttc cacgccaccg
     1981 tggcggacgg catacctggg agaacctggt ggcggcgtgc aagggctgca accaccggaa
     2041 ggccggtcgc acgccgtcag aggcgcggat gctgttgagc agggcgccct accggcccag
     2101 agtgagcagc tattacctgt tctaccagta cctcgacgtc cagcacgaat ggcgcaaatt
     2161 tatcccgggc tgggacgaag ccagcatcgg catctcttga aagcgagcgt gcgttacccc
     2221 agcaggactg cgcgtaaagc gtcctcggtt tccggcgggg taactgcgag cacctggtcg
     2281 ccgtcctgca gcaccgtatc cggggtgggg accttggcaa agccatccga atccacgagc
     2341 agcggaatca gggagcgctg aggcagcagc aggtcgcgca cacgccggcc caccgaagcc
     2401 gaatcctgga ccaattgggc ctctacaagc tccaggccgc tattgcggag cgtaagcaaa
     2461 gggatcaagt ggtgagtggg aagcgcctgt tcgatgtggg cgaggatgac ggcggtggcg
     2521 ctgacagtcg tgtcaatgcc cagctttttg aagatcgcct cgttcttcgg gttgttgata
     2581 cgggcgataa tgcgcgggac gtcgaactta gtgcgcgcca cctggcaggc cacgaggtta
     2641 tcctcatcgc caccggtcac cgcaatgaac atgttggctc ggcccgtccc ggcgttgtcg
     2701 agcaccgcgg cctcgcagcc gtctccgcat aaggtgatgt cgccgagctc gtcggtgatg
     2761 cgctcgcact tgctcggatc ctgctcgatg acaagcagct catgtccttc actgaccagc
     2821 tctcgggcaa ggtaaaagcc gactttgccg ccgccgacga caatgatgta cacggttggc
     2881 cgcctccttg cttagcgttc cagtgcgtcg tggagtagtt gcgcgccgac ttttgtcggg
     2941 ctgatcgttt cgagcccgag ggtgtggtac agctcttccc ggatggggtc gtagatgcgg
     3001 caaacgacgc gcttgacgtt gaacaggtgc tgagcgatct gcgccgccat gacgttgcga
     3061 ttatcgccct gtgtcacggc aacaaacgcg tccgcgtcct ggatacctgc gcgacgcaac
     3121 gtgtccatgt cgatgccgtt accgaccagg gcgcgcccgc tgaagtccga aggcagccgc
     3181 ttgctgaatg actcgctgcg cagatcgagg atggttacgt cgtgaccttc gcggtcgagc
     3241 gtggtggcga gttgcgcacc cacgcggccg cagcccatga tcacgacctt cacaattctc
     3301 tcctcgcgcc acgtcaagta tgtgaaacca aacacatgct aggtatcggt ctgatctctg
     3361 tcaacgcggc aaatcaacac cgaatgccgg ccgagcacgc gcgtcagtac aaggatccgt
     3421 tccccagaac cttctaacct gagggagcgc tccagctcct gcggatctag cggcgaagcg
     3481 cgtgtcttga cgactacgtg gccgatatcg agcgcccgca ggcggcggcg tagctgtttc
     3541 aaattgaacg gcatccactc ctcgacgcgg taagccgtcg cgaacggcgt ttgctgcgaa
     3601 acgtcggcgc ataggaaggc ggtcgagtcg tcgatcttgg tggcgcccag gctgtgggcg
     3661 acctgctcaa tcgcgtgggc gcggatcacg gctggtccgg gttcgaagag gaactgacgc
     3721 ggtggcgccg ccggcacatg cgccacggaa acggtggtga gggtctgccc gcccggcaac
     3781 agggtggcgc gccggcgggt cgtagccagc ggccccagcc agagcaccgc ctgcttgagg
     3841 tcaccacgct cctggatgaa ttcgacttcg cagccggacg gaagcgcttc gtcgcgtatc
     3901 cc
//
LOCUS       DATCPE010000078         2620 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U7219
            k141_468560, whole genome shotgun sequence.
ACCESSION   DATCPE010000078 DATCPE010000000
VERSION     DATCPE010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955033
            Sequence Read Archive: SRR1586279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 16:49:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 640
            CDSs (total)                      :: 631
            Genes (coding)                    :: 627
            CDSs (with protein)               :: 627
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2620
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_468560"
                     /isolate="SMAG_U7219"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /locus_tag="VIM45_02390"
     CDS             complement(<1..326)
                     /locus_tag="VIM45_02390"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEY8820166.1"
                     /translation="MGMIRADLHSHTHFSRDGLTTPDQYVQRSVEHGITCAAVSDHNN
                     IDGAIAVRRLAPFKVIISEEIRSTEGEIIGYFLHDPVPKRLTPEETVRAIREQGGIVG
                     VPHPFDR"
     gene            complement(337..570)
                     /gene="xseB"
                     /locus_tag="VIM45_02395"
     CDS             complement(337..570)
                     /gene="xseB"
                     /locus_tag="VIM45_02395"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020177648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEY8820167.1"
                     /translation="MTEQETFESVYAHLEETVHRLENGGLPLDESIALFEYGMGMAKR
                     CRELLDGAELRITTLTEEFHSAGPPAPEDADLC"
     gene            complement(563..1750)
                     /gene="xseA"
                     /locus_tag="VIM45_02400"
     CDS             complement(563..1750)
                     /gene="xseA"
                     /locus_tag="VIM45_02400"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEY8820168.1"
                     /translation="MVVLTVEQALGYLRELFETNPTLSDLWIMGEISNLTRSSAGHIY
                     FTLKDGAGQIRCAFFKRQNVGVTLEHGQQVLAHGRVSIYEQRGDLNFIVDFVHSQGAG
                     ILHAQFERLKQVLESEGLFDTSRKRPLPAFPRRIGVVTSPAGAVLHDICTVIGRRWPL
                     AEIMVAPTPVQGDAAAPAIVAALQALNERASVDVIIVARGGGSLEELWPFNEEQVQRA
                     IYASRVPVVSAVGHETDFTLADYVADLRAPTPSAAAEMVVPDQIHVAMRLGSYVATLE
                     SCVRHELESNRDRLDRVADYIELAAPDLKAIDERLSALHVQACNQVKKALSGGRALVE
                     LRVLALTTLNPLAVLERGYAVVHHEGGALVARASEAIPGDRLSVRLRDGSFAAEVKER
                     HGD"
     gene            complement(1776..2339)
                     /gene="efp"
                     /locus_tag="VIM45_02405"
     CDS             complement(1776..2339)
                     /gene="efp"
                     /locus_tag="VIM45_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008476601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEY8820169.1"
                     /translation="MITPGELKRGIAIELDGKLYTVLSYEHNKVGRGSAQIRIKLRDV
                     RAGHTIDRTFQASEKFVRARIDKSPMQFLYEENGLYYFMNAATYDQIALTKDQVGDAL
                     MYLKENETCEVLTHGDDAIGIELPTSVSLKVVDTEPGFRGDTATGGNKPAKLETGLTV
                     QVPLFIKEGDTLKVDTRTGDYLERVAQ"
     gene            complement(2336..>2620)
                     /locus_tag="VIM45_02410"
     CDS             complement(2336..>2620)
                     /locus_tag="VIM45_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013278704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEY8820170.1"
                     /translation="YAHKLAQRVIEEAGYGEHFGHGLGHGIGLQVHEAPRLAPKSTSV
                     LQEGQVFSVEPGIYLSGWGGVRIEDLVVLENGRCRVLSKSPKIRLSGVTA"
BASE COUNT          473 a          800 c          814 g          533 t
ORIGIN      
        1 cgatcgaacg gatgcggcac gccaacaatt ccaccctgtt cgcggatggc gcgaaccgtc
       61 tcctccggcg tcaaacgctt tggtacgggg tcgtgcagga aatagccgat gatctcgccc
      121 tcggtggaac ggatctcctc cgagataatg accttgaatg gagcgaggcg gcgtacggcg
      181 atcgcaccgt cgatgttgtt gtggtcgctg accgcagcgc aggtgatgcc gtgttcaacg
      241 gaccgttgaa cgtattgatc gggcgtggtc aggccgtcgc gggaaaagtg ggtatggctg
      301 tgcaggtcgg cgcgaatcat gcccatgttc tggcgctcaa caaaggtcgg cgtcctcggg
      361 agcaggcgga cccgccgaat gaaactcctc cgttagcgtg gtaatgcgca gctcggctcc
      421 gtccagcagt tctcggcagc gtttggccat gcccatacca tattcgaaga gcgcgattga
      481 ctcatccaac ggtagaccac cgttttcgag acggtgaacg gtttcttcca ggtgtgcata
      541 gaccgactcg aaggtctctt gctcagtcac catgccgttc cttcacttca gcggcaaacg
      601 aaccgtctcg cagccgcacc gacagccggt ctcccgggat ggcttcggat gccctcgcca
      661 caagtgcacc accctcatgg tgcacgacgg cgtaaccgcg ttccagcact gccagcggat
      721 taagcgtggt caacgccaga acccgcagct ccaccaatgc tcgtcctcct gacagcgcct
      781 ttttcacctg gttgcatgcc tgaacgtgta gggcggacag ccgttcgtcg atcgctttca
      841 gatccggggc ggctagctcg atgtagtccg ccacacggtc caaccggtcg cgattgcttt
      901 ccagctcatg acgcacgcac gattcgagtg ttgcaacgta cgacccgagt cgcattgcaa
      961 catggatctg gtctggaacc accatttcgg ccgccgcgga cggcgttggg gcgcggagat
     1021 cggcgacata atcggcgagc gtaaagtcgg tctcgtgccc gactgccgac acgacgggaa
     1081 cgcggctggc gtagatcgcg cgttgcacct gctcctcgtt gaagggccac agctcttcga
     1141 gtgagccacc gccgcgcgcg acgatgatca catccacgct tgcgcgctcg ttgagcgctt
     1201 gcagggcggc gacgatcgcg ggcgccgcgg cgtccccttg cacgggagtg ggtgcgacca
     1261 tgatctccgc gagtggccag cgccgcccga tcacggtgca gatgtcgtgg agcaccgcac
     1321 cggccggcga ggtgacgacg ccgatacgtc gagggaaggc ggggagcggc cgcttgcgcg
     1381 acgtgtcgaa cagaccctcg gactcgagta cctgcttcag gcgctcgaac tgcgcatgga
     1441 ggatgccggc gccctgcgag tggacaaaat cgacgatgaa gttcagatca ccgcgttgct
     1501 cataaatgga aacgcgtcca tgggccagca cctgctgtcc gtgctccagc gtcacgccca
     1561 cgttctggcg cttgaagaac gcgcaacgaa tctggcccgc gccgtctttc agcgtgaagt
     1621 agatatggcc cgctgatgag cgggttaggt tcgagatttc gcccatgatc cagagatcgc
     1681 tgagcgtggg gttggtctcg aacagctctc ggaggtagcc gagggcctgc tcgacggtca
     1741 gcacgaccat ggcgggatcg gctccgtgtc aggctttact gcgctacgcg ctccagataa
     1801 tcgccggtgc gggtgtctac tttgagcgta tcgccttcct tgatgaaaag cggtacttgc
     1861 acagtcagcc cggtctccag cttggccggc ttattgccgc ccgtggccgt gtcgcctctg
     1921 aagccgggct cggtgtccac gaccttcaga ctgaccgacg tcggtagctc gatcccgatg
     1981 gcgtcgtctc cgtgggtcag cacttcgcag gtctcgtttt ccttcaggta catgagcgcg
     2041 tcgccaacct gatccttggt aagcgcaatc tggtcgtagg ttgccgcgtt catgaagtaa
     2101 taaaggccgt tctcttcgta gaggaactgc atcggcgatt tgtcgatacg ggccctcacg
     2161 aatttttcgg acgcctggaa ggttcggtcg atggtgtggc cggcgcgcac gtcgcgaagc
     2221 ttgatgcgga tctgggcgct gcccctgccg accttgttgt gctcgtagct caagacggta
     2281 tatagcttgc cgtcgagttc gatggcgatg cctcgcttca gttcgccggg cgtaatcatg
     2341 cagtgactcc tgacaatcgg atctttggcg atttgctgag aacgcggcag cggccattct
     2401 ccaggaccac caggtcctcg atgcgaacac cgccccagcc gctcagatag atgcccggct
     2461 cgacggagaa cacctgcccc tcctgaagca cagaagtgga tttcggggca aggcgcggtg
     2521 cttcgtggac ctgaagtccg atgccgtgcc caaggccatg gccgaagtgt tcgccgtaac
     2581 cggcctcttc gatcactctc tgcgcaagct tgtgggcgta
//
LOCUS       DATCPE010000079         4187 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U7219
            k141_210079, whole genome shotgun sequence.
ACCESSION   DATCPE010000079 DATCPE010000000
VERSION     DATCPE010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955033
            Sequence Read Archive: SRR1586279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 4187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 16:49:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 640
            CDSs (total)                      :: 631
            Genes (coding)                    :: 627
            CDSs (with protein)               :: 627
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4187
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210079"
                     /isolate="SMAG_U7219"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            136..789
                     /locus_tag="VIM45_02415"
     CDS             136..789
                     /locus_tag="VIM45_02415"
                     /inference="COORDINATES: protein motif:HMM:NF018712.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1326 domain-containing protein"
                     /protein_id="HEY8820171.1"
                     /translation="MTAPSSPTGVSAAHGGNVPQWNIRGQVILACNCDYGCPCNFNGL
                     PTTGKCEGNWNWHIEHGNFGDVPLSGLTVGVAVNWPGAIHEGNGQAMAVLDERADERQ
                     RQALETLVSGEAGGPWKIINTTIGKMSAPEYAPFEVTLNDYTSSVKAGSYITLQMQPV
                     RNPVTKAEVHPRVVLPEGFVFKEGQVGASSTFRIAGPVSFDHSGRYAAVGPFDYQGP"
     gene            807..1637
                     /locus_tag="VIM45_02420"
     CDS             807..1637
                     /locus_tag="VIM45_02420"
                     /inference="COORDINATES: protein motif:HMM:NF021465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2182 domain-containing protein"
                     /protein_id="HEY8820172.1"
                     /translation="MAASPATVVLGAALIAVAATAWLLVLRAGSSMSMMGPGASPSVA
                     EGAAFTAQWGVMMAAMMLPSAAPMILLYRTVSKRLSAERNPSIPVALFAAVYLFLWLL
                     LGAPVYGAYVAVSSLAARWRPFDAAMPYAVAAVLVAGGVYQLTEAKRVCLRHCEAPLG
                     FLMRRWRSGYAATLRLAVEHATYCIGCCWALMAILVAAGAMSISWVLAIAIVVFAEKV
                     LPRGWRTARLVGVSLIALGVAVALHPELAGTMRPHAAPMGVDSRGPLPRSYLSVGVTE
                     "
     gene            complement(1684..2658)
                     /locus_tag="VIM45_02425"
     CDS             complement(1684..2658)
                     /locus_tag="VIM45_02425"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY8820173.1"
                     /translation="MTKRTILIVDDHITVARELEGRLIRLGYEVVAIASSGDEAIIAM
                     TAQAAPDLVLMDIALRGDTNSVETANELQRYGKIPVVLMIAQTDEAIVPRAGVSYPYG
                     YVVKPFSERELRVNIELALCKHDAARAVHQLEDRFFAASIDLLCLLDFNGYFKRVNPA
                     WEQTLGFTREELMSRPFIEFVHPDDRERTLKQNAQVRGGGQALAFENRYLCKDGSHRW
                     FHWNAAPDSAEQVIYSMARDITASKQAEDEREQLVRELQAALAEVKTLQEILPICSYC
                     RKIRDDENYWHTVENYISRHTTTRFSHGICPSCMATNVESQLEARERE"
     gene            complement(2844..3302)
                     /locus_tag="VIM45_02430"
     CDS             complement(2844..3302)
                     /locus_tag="VIM45_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HEY8820174.1"
                     /translation="MTLWIDADAAPQPVKEICFRASERLTLATVLVANQRLPLPSGYS
                     YVSAVRVEGGPDVADRYIAQHAALGDVAVTADIPLAALLVPKGVIVLDPRGEEYTGES
                     IGERLSVRNFMDKLRTTGVDTGGHAPYGPREKQAFANALDRALTRALKQR"
     gene            3576..>4187
                     /locus_tag="VIM45_02435"
     CDS             3576..>4187
                     /locus_tag="VIM45_02435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8820175.1"
                     /translation="MAVAESAAAAPKERATWQRRLDASHDTLENVLAWYTSHDQGNEA
                     LRLLVALERFWNGPSLVTGFDQALAIPGTQPRIRARALNSASHAAFRVKDQERTRRWA
                     NESIAVWRSLGDSAQTGRAYERLVQAALREDNHAALRALADTGELLCVQAHDEDCHAY
                     FLNMRGESARVLKQYDSAAVYYGQAAVLYSRVSPVPRLDIAHNI"
BASE COUNT          751 a         1307 c         1308 g          821 t
ORIGIN      
        1 gctccaggca tggtagcaag tagcgttgac aaacgcttac gatctccata gcgtcctgcg
       61 cgattaccgc catccggtga ggtccgtcgt gatggcggat actactcatc ctctcaccac
      121 gaccaggtac gcaacatgac ggcgccttca tcccccaccg gcgtctccgc cgcgcatggc
      181 ggcaacgtgc cccaatggaa tattcgcggc caagtaatcc tcgcctgcaa ctgcgactac
      241 ggctgcccct gcaacttcaa cggccttcca acgactggta agtgcgaggg aaattggaac
      301 tggcacatcg agcacgggaa cttcggcgac gtgccgctct ccggactcac cgttggtgtt
      361 gccgtgaact ggccgggcgc cattcacgaa ggtaatggtc aggcgatggc cgtcctcgat
      421 gaacgtgcgg acgagcgaca gcggcaagcg ctagagacgc tggtttccgg tgaggcgggc
      481 ggaccgtgga agatcatcaa tacgaccatt ggcaagatga gcgcgccgga gtacgcgccg
      541 ttcgaagtta ccctcaacga ctacaccagt agcgtgaaag cgggcagcta catcacgctt
      601 cagatgcagc ccgtgaggaa tccggtgacg aaggccgaag tgcacccgcg cgtcgttcta
      661 cccgaagggt tcgtgttcaa ggagggacag gtgggcgcgt catccacgtt ccgcattgcg
      721 ggaccggtga gtttcgatca ctcgggacgc tacgccgccg tcggcccatt cgactatcag
      781 ggaccgtgag gcgggcacgg tcggctgtgg ccgccagccc agccaccgtc gtcctgggtg
      841 ccgcgctgat cgcggttgcg gcaacggcgt ggctcctcgt gttacgagcc ggctcttcga
      901 tgtccatgat gggaccgggc gcatctcctt ccgtcgcaga gggcgcagcg ttcaccgcgc
      961 agtggggcgt gatgatggcc gcgatgatgc tgccgagcgc cgcgccgatg attctgctct
     1021 accggacagt cagcaagcgt ctgtcggccg agcgcaaccc gtcgatcccg gtggcactgt
     1081 tcgccgccgt atacctattc ctttggctgt tattgggggc tcctgtctac ggagcctacg
     1141 tcgcagtgtc gtccctggct gcgcggtggc ggcctttcga cgccgcgatg ccgtacgcgg
     1201 tagccgcagt tctggtcgcc ggcggggtct accagctcac ggaagcgaag cgcgtctgcc
     1261 tccgccattg cgaagcgcct ctcggattcc tcatgcggcg gtggcgtagc gggtatgctg
     1321 cgacgctgcg gcttgcagtc gagcacgcga cctattgcat cggatgttgc tgggccttga
     1381 tggcaatcct cgtggctgcc ggtgccatga gtatctcatg ggtgctggcg atcgccatcg
     1441 tcgtatttgc ggagaaggtg ctgccgcgcg gctggcggac tgcgcgattg gtgggagtga
     1501 gcctgatcgc ccttggggta gcggttgcgc ttcacccgga gttggcgggc acgatgagac
     1561 cgcacgccgc gcccatggga gtcgactctc gggggccgct gccccgatcc tatctatccg
     1621 tcggcgtgac ggagtgatgg ccatcgtgag agccgaaacc gttggcgccg caccgggcct
     1681 cccttactcg cgttcccgcg cttcgagctg cgactcgaca ttagtcgcca tgcagctcgg
     1741 acagataccg tggctgaacc gggttgtcgt gtgccgagag atgtagttct cgacggtgtg
     1801 ccagtagttc tcatcgtcac ggatctttct gcaatacgaa cagatcggca gtatctcctg
     1861 caaggttttt acttcggcca gcgcagcctg tagctcgcgc accaattgct cgcgctcgtc
     1921 ctccgcctgc ttgctcgcgg tgatgtcacg cgccattgag tagatgacct gctcggcgga
     1981 atccggggcc gcgttccaat gaaaccatct atgcgagcca tccttgcaga gatagcggtt
     2041 ctcgaaggca agcgcttgtc caccgcctct tacctgggcg ttctgcttca gcgtgcgctc
     2101 gcgatcatcg gggtgcacga actcgatgaa cggccgcgac atcagctcct cgcgggtgaa
     2161 gccgagtgtt tgctcccacg ctggattgac ccgcttgaag tatccgttga agtcgagtaa
     2221 gcagagcaag tcgatcgagg cggcgaagaa gcgatcctcc agctgatgga ccgctctcgc
     2281 cgcgtcgtgc ttgcagagag ccagctctat gttgacgcgc agctcccgct ccgagaaagg
     2341 cttcacgacg tagccgtatg ggtagctcac tccggcccgt ggcacgattg cctcatccgt
     2401 ctgcgcgatc atgaggacga ccgggatctt cccgtaccgc tggagctcat tggcggtttc
     2461 cacgctattc gtgtcgccac gcagcgcaat gtccatcagc accaggtccg gcgcagcctg
     2521 agccgtcatg gcgatgatgg cctcatctcc ggaagaggcg atcgccacga cctcgtatcc
     2581 gagcctgatg agccgcccct cgagctctct ggcgacggtg atatggtcat ccacgatcag
     2641 gattgttcgc ttagtcacaa ggtgcccggc tcctccatct gcaacgaagt gcggggaagg
     2701 cgatttgaag cggcgacacg gccgccggag atgccctcaa tttatcctgt ttcgaagcag
     2761 gtatgctggt gcatctcata ccgccgcata ttgacgatct aatacacggg cagatcagcc
     2821 tgaggcattg ggccgcgacc cgctcagcgc tgtttgagcg cgcgcgtgag cgcccggtcg
     2881 agtgcgtttg cgaatgcctg tttctcgcgc ggtccatagg gcgcgtggcc acccgtgtcg
     2941 acaccggtcg tccgtagttt gtccatgaag ttccgcaccg acagtcgctc cccaatgctt
     3001 tcgccagtgt actcttcacc cctggggtca aggacgatta cccctttggg caccaggagg
     3061 gcggcgagcg ggatgtcggc tgtgacggca acatcgccga gggcggcgtg ctgcgcgatg
     3121 tagcggtcgg ccacgtccgg cccgccctcg acgcggacag ccgacacgta cgagtatccg
     3181 gacggaagtg ggagacgctg gtttgccaca aggacagttg cgagcgtgag ccgctcagag
     3241 gcgcgaaagc agatttcctt cacgggctgc ggggcggcgt cggcgtcgat ccagagcgtc
     3301 atgaccacga acttgtcacc gggtatcgaa caagagagag actcgctgcg ttcgcgggct
     3361 ggcgaaagat aatcctgcgc gccatagtgg gcactcaatg actgggcgtt tttcgtggtc
     3421 ccgctaaaaa gaggaaacga tccatgcgcc agcgtcttgt agttctcgcg ggtttcgcaa
     3481 cgctcacgct gccgtacaca caagccctgt acggccagag cccgtcgcaa cagcttccac
     3541 aggtagctat aaccgaggga gtctccgcgc actacttggc agttgcagaa agtgctgccg
     3601 cagcgccgaa ggagcgcgcc acttggcagc gccggctcga tgcctctcac gacacgcttg
     3661 aaaacgtgct tgcctggtac accagccacg accaaggcaa cgaagcacta cgacttcttg
     3721 tggccctgga gcgcttttgg aacgggccat cgctcgtcac cggattcgac caggcactgg
     3781 cgattcccgg cactcagccg aggattcgcg cccgcgcatt gaacagcgcc tcccatgcgg
     3841 catttcgagt aaaagatcag gagcgtactc gccgctgggc gaatgaaagc atcgccgtct
     3901 ggcgctcgct tggggattcg gctcagaccg gccgtgcata cgagcgcctg gtacaggcgg
     3961 cactccggga ggacaaccac gcagcgcttc gggcgctagc cgacaccggc gagttgctgt
     4021 gcgtccaggc acatgatgaa gactgtcacg catacttcct caacatgcgt ggcgaatcgg
     4081 cgcgagtcct caagcaatac gattctgccg cggtgtacta cggtcaggct gccgttctct
     4141 actctcgcgt ctcacccgtg ccccggctgg acatcgccca caacatc
//
LOCUS       DATCPE010000080         9204 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U7219
            k141_437445, whole genome shotgun sequence.
ACCESSION   DATCPE010000080 DATCPE010000000
VERSION     DATCPE010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955033
            Sequence Read Archive: SRR1586279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 9204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 16:49:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 640
            CDSs (total)                      :: 631
            Genes (coding)                    :: 627
            CDSs (with protein)               :: 627
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9204
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_437445"
                     /isolate="SMAG_U7219"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..269
                     /locus_tag="VIM45_02440"
     CDS             <1..269
                     /locus_tag="VIM45_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8820176.1"
                     /translation="QLDLNGIAASSGSACTSGSLEASHVLQAMGVPIEIARSSLRLSV
                     GVENSLEQIEQVLDVLPRFIERLRALSPLGPDTPRPDLVTAEQP"
     gene            343..987
                     /locus_tag="VIM45_02445"
     CDS             343..987
                     /locus_tag="VIM45_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012059187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HEY8820177.1"
                     /translation="MSAVVPLHLADERPLHLDIDQELKNNLLVSSVDTVLNWARSSSL
                     WPAMFGLACCAIEMIASATSRYDIARFGAEIFRASPRQADFMIVAGTVTWKMAPAVRR
                     VYLQMAEPKWVIAMGGCAIMGGPFAYGYSTLPGVNLIIPVDVYVPGCPPRPESLLTGL
                     MMLQEKIKHESVVGKRERIEPTGDFNQFLPADDPVRRELEMLFPPYLKTGADAV"
     gene            1286..2893
                     /gene="groL"
                     /locus_tag="VIM45_02450"
     CDS             1286..2893
                     /gene="groL"
                     /locus_tag="VIM45_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874516.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEY8820178.1"
                     /translation="MAKQLLFDEEARHALRRGIDALADAVKITLGPKGRNVVLDKKFG
                     SPTITNDGVTIAKDIELEDPFENIGAQLAKEVASKTNDIAGDGTTTATVLAQAIVHEG
                     LKNVAAGANPMALKRGLESATSVIAQAIRDNATPVTTREQMAQVASISAADQEIGDMI
                     GEVMEKVGKDGVVTVEESKGIRTETEYVEGMSFDRGYISPYFVTNPERMEAVIDDPYI
                     LITDKKISAVNDILPLLERLLQVSKNFVIIADDVDGEALATLAVNKLRGTINVLAVKA
                     PGFGDRRKDNLGDLAVLTGGQVISDELGRKLDSVTPADMGRARRVICTKEETTVIEGK
                     GEQTAVEARVSQVRAMMEDAKSDWDKEKAQERLGKLAGSVAVIKVGAATEIELKEKKH
                     RVEDALSATRAAVEEGIVPGGGVAFINALPKLEALQLGDDESTGGNILRRALEEPLRR
                     IAINAGQDGSVIVQEVRSLPVGQGYDALLGEFGDMIKKGIIDPAKVTRSALENAVSIA
                     GMVLTTNCLVTDLPEKQAGAPGAPPMY"
     gene            3037..4500
                     /locus_tag="VIM45_02455"
     CDS             3037..4500
                     /locus_tag="VIM45_02455"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR02095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA];
                     GO_function: GO:0009011 - starch synthase activity
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase"
                     /protein_id="HEY8820179.1"
                     /translation="MKVLFVTVEMSPLAKVGGLADVAGSLPKALVRSGHDVRIVMPLH
                     GITDRSRFGVTSTGETVIVRQAAGSELAGEICMATVGGVPVYLVDVPALFDRTQIYGE
                     QDDTVRWLAFCDAVLAWLRRIDWRPDVLHLNEWHTAFIASRLRQDPDDALAALPRVYT
                     IHNLAIHGGFDDEFARAAGIGGRALNSPLALEPWVSRSGMGQGLLWCDLINTVSTTYA
                     KEIMEPEYGAGLDPLLRKRRDDLFGILNGIDYEEFDPATDPRLPAHYDADQYAGHSDV
                     KAALQRRMGLIETRETPVIGMVTRLFFQKGADLAVESIEALINEHPLQFVVLGTGDNV
                     YHEQLVALRERHPGKIALELAFDADLAQLIYGGTDVFLMPSRFEPCGLGQMIALRYGS
                     VPVVRRTGGLADTVREWTGPGSGGNGFLFDLPAADELTQALHRALAVYARPDEWQALV
                     RSGMRDDYSWGEAAREYVGLYERSVGRAASSSNPART"
     gene            4526..5503
                     /locus_tag="VIM45_02460"
     CDS             4526..5503
                     /locus_tag="VIM45_02460"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEY8820180.1"
                     /translation="MPGILAGAIDLGGTKILSLVVDDGGAIKGRDQRPTQAERGLDRV
                     LGSMVESLRRACEDGGVGLDALHGIGVAVPGPIDLEAGVLASPPNLPGWNDVPLVRLL
                     QERTRISVLLENDANAAAVGEHAFGAGRGIRDMVFITISTGIGGGIIAGGHIYHGATG
                     AAGEIGHVVVEPDGARCGCGRRGCLEGLASGTAIGRNGEHAARQGRSSALGEVLDKVG
                     AVTAEDVSRAAQGGDTGAKEVLATAAKYLGIGLVTVVHLLNPQMIVIGGGASKIGDPL
                     LGPAEAYMRSHAFPLMAAAVRIVPASTGDDAGALGAAALVLNRDLAESA"
     gene            5592..6872
                     /locus_tag="VIM45_02465"
     CDS             5592..6872
                     /locus_tag="VIM45_02465"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8820181.1"
                     /translation="MTAPPVEPSLQAPGRVFSRLRDQSPAAAGLIAVIFLTEAVIGVL
                     LLAINPQYPASRLGEGVRITGLAFSVYGAAKMPGQPIGGWLADRFEPRIVLIGGLLLS
                     LPTIIIMERFQNVWAYILAWIMFGLTLAVVWPAVYAIVGHRFRPDRQGRLLALVSMGQ
                     IAGTAAGTGAGAFVVDHVSYGGAFGLALLIEAIALSIAILAVPRTRVDGPTSLVPGTA
                     LPPSGFSSIRRLVTLNVAMLIGLIMLLSTAVAMIAPDLKPYSDQILHVSYSTFVLMLA
                     IPATVAALLIVPSGYIADRFGRNLPIGLGLFLFAASLLLLTLTHSPLLSTLFATIAAV
                     GYVLVLPAFSASLLDLSSSGNRGLLTGISTSIQAIGLVVGPAVGGFLIDASGPLAPFR
                     VAFAIVFVTGLLSIVYAGRTRAIYRERVQRAGGA"
     gene            complement(6951..7763)
                     /locus_tag="VIM45_02470"
     CDS             complement(6951..7763)
                     /locus_tag="VIM45_02470"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEY8820182.1"
                     /translation="MTEPLRVLGIFPHPDDESYSSGGTLARIAAEGAGVHVLCATAGS
                     AGMDLRANVAPVNDLGATRAAELACACDALCLPPPAFLGLPDGGVGDVNFPDVAGEII
                     REIRSVRPQVVITLGADGVYGHPDHLALYRLVVAAFGAAGGGDRFPESEFGPGWAPQR
                     LYLAAFPRGMFRPMFDHMLESEYATTIRGLDPGKLGVEPQDVAAAIDIREFSDRKLAA
                     IACHRSQLRDGDPNTLFPNGLVQRTLTTELFTLGAGKPVSRRLRNLSEDLEL"
     gene            complement(7771..>9204)
                     /locus_tag="VIM45_02475"
     CDS             complement(7771..>9204)
                     /locus_tag="VIM45_02475"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8820183.1"
                     /translation="VFFGTLDQTVVVTVLPDIIKDIRLPINTKFGEASWIVNGYLIGY
                     TVSMPIVGRIADVFGRVRIFLVAIAIFVAGSLMVAVAPNLAFLVAARALQAIGGGGLL
                     PIALAIAADATSTKRRTLVLGALAAANNGSTFLGPVWGAAISGAWNWRVIFWLNVPLL
                     LPFMVAMPLLARDHRTGERQRIDWRAAVLLVAGLSALTFALTDDGANPRPLPVSLAVG
                     TAGAIALALFARAEWLNPRPMVDLRILRVRHVSAAMLTYFLIGGALITALVNVPLMTD
                     VLYGGSTTQGGLNLMRLLLLLPIGGLVGGWLATMVGNRPTVLLGLGFAIAGFIWMRAW
                     PFVPGEAAALAPSTWHLWGALGAIGLGLGLCDGPIVATVVDAVSHRERATASALLLVV
                     WTTGMIVGLALLATQGLGTFTRRLADISFDDPNYQARFQGIMHRTFDETFLVAALALG
                     VAFLLSLALQRGRAQEIIVAPYEGLGE"
BASE COUNT         1733 a         2897 c         2923 g         1651 t
ORIGIN      
        1 tacaactgga cctgaacggc atcgccgcgt ccagcggctc ggcctgcacc tccggctcgc
       61 tcgaagcctc gcacgtgttg caggcgatgg gcgttccgat cgagattgcg cgcagcagcc
      121 tccggctcag cgtcggcgtc gagaattcgc ttgagcagat cgaacaggtt ctcgacgtgc
      181 tgccgcgctt catcgagcgc ctccgtgcgc tttcgcccct cggtccggat acgccgcgac
      241 cagacctcgt gacggccgag cagccctgat gttacgatgc ttgtgatagc gggaacgaag
      301 gcgattcact gatgaatcgc caagtccgta tcgggagccg gtatgtccgc tgtagtgccg
      361 cttcatctgg ccgacgagcg cccgctgcac ctggacatcg accaggagct gaagaacaac
      421 ctcctggtct cttccgtgga tacggtgctg aactgggccc gtagctcctc gctttggccg
      481 gcgatgttcg ggctcgcctg ctgtgcaatt gaaatgatcg cgagcgccac ctcgcgctat
      541 gacatcgcac ggtttggagc cgagatcttc cgcgcgtcac cccggcaggc cgatttcatg
      601 atcgtggccg gtaccgtgac ctggaagatg gctccagctg tccggcgcgt ctacttgcag
      661 atggcggagc caaaatgggt gatcgccatg ggcggttgcg cgatcatggg cgggcccttt
      721 gcctacggtt acagcacgct ccctggcgtg aacctgatca tcccggtgga cgtctacgtt
      781 cccggctgtc cgccgaggcc cgaatcgctg ctcaccggcc tgatgatgct ccaggagaag
      841 atcaagcacg agagcgttgt tggcaaacgc gagcgcatcg agccaacggg cgacttcaac
      901 cagtttctgc ccgccgacga cccggtgcgg cgtgagctcg agatgctatt tccgccatat
      961 ctgaagaccg gcgccgacgc cgtctgatcc ggctcggaaa cgaccaggac ctgcgaggct
     1021 cgtccatccg agcggccctc ttgcttttcc gaccggcgtg attcggtttc aacttcgaag
     1081 ctgccaccaa cccgatcgag ccgtgaaaat gtgcgccaca gagtgagtta gaatgcgaga
     1141 gacagagtga gcctggcaga gcattcgggc gctgtgcgga gttctaccgt aggcgctctg
     1201 cttgctgcga ttcggctgct ccgatacaac tggtttagca ctcgtatagt gagagtgcta
     1261 attttgaaca gtgtacaggt gagcaatggc gaaacaactg ctttttgatg aggaagcgcg
     1321 acacgccctc cgccgcggca tcgacgcgct tgccgatgcc gtgaagatca cgctaggccc
     1381 caaaggacgc aacgtcgtcc ttgataagaa gttcggctcc ccgacgatca ccaacgacgg
     1441 tgtgacgatc gccaaggata tcgagctcga ggatcccttc gagaatatcg gcgcccagct
     1501 tgccaaagaa gtcgcttcca agaccaacga catcgcaggc gacggcacca ccacggcaac
     1561 cgttcttgcc caggcgatcg tccatgaggg tctcaagaac gtcgcggccg gcgccaaccc
     1621 catggctctg aagcgcggcc tcgagtccgc cacgagcgtg atcgcccaag cgattcgcga
     1681 caacgcgact ccggtaacga cccgcgagca gatggcgcag gttgccagca tctccgcggc
     1741 cgaccaggaa attggcgaca tgatcggcga ggtcatggag aaggtcggta aggatggggt
     1801 cgtaaccgtc gaagagtcga agggcatccg caccgagacc gagtacgttg aaggtatgag
     1861 ctttgaccga ggctacatct cgccctactt cgttaccaac ccggagcgga tggaagcggt
     1921 catagacgac ccctacattc tcatcaccga caagaagatc tccgcggtca acgatattct
     1981 gccgctgctg gagcgactgc tccaggtaag caagaacttc gtgatcatcg ccgacgacgt
     2041 cgacggtgag gcgctcgcta cgctcgcggt caacaagctg cgcggcacga tcaacgtgct
     2101 ggcggtgaag gcgccgggat tcggtgaccg ccgcaaggac aacctgggcg acctcgccgt
     2161 tctgaccggc ggccaggtca tcagcgacga gctcggacgg aagctcgatt cggtcacgcc
     2221 ggcagacatg ggccgcgctc gccgcgttat ctgcaccaag gaagagacca cggtgatcga
     2281 gggcaagggc gaacagacgg ccgtcgaggc gcgtgtctct caggtccgcg caatgatgga
     2341 agatgccaag agcgactggg acaaggaaaa ggcccaggag cggctcggta agctggccgg
     2401 cagcgtcgcg gtcatcaagg tcggcgcggc caccgagatc gagcttaaag agaagaagca
     2461 ccgcgtcgaa gacgcgctct ccgcgacgcg tgccgcggtt gaggaaggga tcgtaccggg
     2521 cggcggcgtt gccttcatca atgcattgcc gaagctggag gcactgcaac tcggcgacga
     2581 tgagtcgacc gggggcaaca tcctgcgccg cgctctcgaa gaaccgctcc gccgcatagc
     2641 gatcaatgcc ggtcaagacg gctcggtcat cgtccaggaa gtccggagcc tgccggttgg
     2701 ccagggctat gacgccctcc tcggcgaatt cggcgatatg atcaagaagg gcatcatcga
     2761 ccctgccaag gtgacccgct cggcgctcga gaacgcggtc agcattgcgg gcatggtgct
     2821 taccacgaac tgcctggtga ccgacctgcc ggagaagcag gccggcgctc ccggcgcacc
     2881 accgatgtac taatcacggg ccaacgggga ttccctcccc aatcagacgg tacaatgcag
     2941 ggggacacgc ggtgcgtgtc cccctgtttc gcgttcgccg cgcggtcgtc agagcaaaca
     3001 gacgaaccgc gagttgtacc tcgcgcagga gttcgcgtga aagttctttt cgttacggtc
     3061 gaaatgagcc cgctcgccaa agtcggtggg ctcgcagatg tcgcggggtc gctgcctaag
     3121 gcgctggtgc gttctggaca tgacgtgcgc attgtcatgc cgctgcacgg gatcacagac
     3181 cgctcccgct tcggcgtgac cagtaccggc gaaaccgtga tcgttcgcca ggcggcgggc
     3241 agcgagcttg ccggcgaaat atgcatggct acagtcggcg gcgtgcccgt gtacctcgtc
     3301 gacgtcccgg cgttattcga ccgcacgcag atatacggag aacaagacga tacggttcgg
     3361 tggctggcat tctgcgacgc cgtgctggcg tggctccgcc gcattgactg gcgaccggac
     3421 gtattgcatc tgaacgaatg gcacaccgcc ttcatcgcca gccggttgcg tcaagatccg
     3481 gacgacgcgc tcgcggcgtt gcccagggtg tacaccatcc acaacctggc catccacggc
     3541 ggcttcgatg acgagtttgc acgggcggcc ggcattggag gccgcgcgtt gaactcgccg
     3601 ctcgccttgg aaccgtgggt gtcccgcagc gggatgggac agggtttgct ctggtgcgac
     3661 ctcatcaaca ccgtcagcac cacgtacgcc aaggagatca tggaaccaga atacggcgcc
     3721 ggtctcgacc cgctgctacg gaaacgacgc gacgacctgt tcggcatcct gaacggcatc
     3781 gactacgaag agttcgaccc ggcgacggat ccccggctac cggctcacta cgatgcagac
     3841 caatatgccg gccattcgga cgtaaaagct gcgctccagc ggcgaatggg actgatcgaa
     3901 acgcgcgaga cgccggtgat cggcatggtc acgcgactct ttttccaaaa gggggctgat
     3961 ttagcggtag agtccatcga agcgctcatc aatgagcatc cgctgcagtt cgttgtgctc
     4021 ggcaccggcg acaatgtcta tcacgagcag cttgtggcgt tgcgtgaacg gcatcctggc
     4081 aagattgcgc tcgagctggc attcgacgcg gacctggcgc aactgatcta cggcggcacc
     4141 gacgtgtttc taatgccgtc tcgcttcgag ccatgcgggc tgggccagat gatcgccctt
     4201 cggtatggct cggtgcctgt cgtgcggcgg acgggcggcc tggcagacac agtgcgcgag
     4261 tggaccggtc ccggtagcgg cggcaacgga tttctcttcg acctgcccgc ggcggacgaa
     4321 ttaacgcagg cgctgcaccg ggcgttggcg gtctatgccc gccccgacga atggcaggcg
     4381 ctggtacgta gcggcatgcg cgacgactat tcgtggggag aggcggcgcg agagtacgta
     4441 ggactctacg agcgatcagt gggccgcgcc gcgagtagtt ctaatccggc gcgcacctga
     4501 acggcgcggt gtacgggaga aaatcatgcc cggtattttg gcgggggcaa tcgacctcgg
     4561 cggcaccaag attctgtccc tcgtcgtcga cgacggcggc gccataaaag gcagagacca
     4621 gcgccccacc caagccgaac ggggactgga tcgtgtgctc ggcagcatgg tcgaatcttt
     4681 gcggcgcgcc tgcgaggacg ggggcgtcgg gctcgatgcg ttgcatggta tcggtgttgc
     4741 cgtgccggga ccgatcgacc tcgaagccgg cgtgctcgcg agcccgccga acctacccgg
     4801 ctggaacgac gttcccctgg tgcgcttgtt gcaggaacgt acccgcatct ccgtcctgct
     4861 ggagaacgac gcaaacgcgg ctgcagtcgg tgaacacgcc ttcggagccg ggcgcggcat
     4921 ccgggatatg gtcttcatca cgatcagcac cggtattggc ggcggcatca ttgccggtgg
     4981 ccatatctac cacggagcca cgggcgcggc cggcgagata ggacacgtgg tcgtcgagcc
     5041 agacggggcg cgttgtggat gcggcagacg cggctgcctg gaaggactcg cctccggaac
     5101 cgccatcggc cgcaacggcg aacatgccgc tcggcagggc cggtcctccg cgctcggcga
     5161 ggttctggac aaggtcgggg cggtgacggc ggaggacgtt tcccgcgcgg cgcagggtgg
     5221 agacacgggg gcgaaagagg tgctcgctac cgcggccaaa tatcttggga tcgggctggt
     5281 gaccgtcgtt catctgttga atccccagat gatcgtcatt ggaggcgggg ccagcaagat
     5341 cggcgatccg ctcctcggtc cggccgaggc gtacatgcga tcgcatgcgt ttcctctcat
     5401 ggcagccgcg gtacgtatcg tcccggcttc tacaggcgac gatgccggcg cccttggggc
     5461 ggcggcgctg gtcttgaacc gtgaccttgc cgagtccgct taggagttca ttctcgtgac
     5521 ccggcggcgc gccgccgcgt atactgtccc ccgacgcgga gctccttcct caatctccgg
     5581 gagacgtcga cttgacagcc ccacctgtcg aaccatcgct gcaagctccc ggccgagtct
     5641 tttcacggct gcgcgaccag agtcctgcag cggccggact catcgcggtg atcttcctca
     5701 cggaggcggt cattggcgta ctcttgctgg cgataaaccc acagtatcct gccagcaggc
     5761 tgggtgaagg cgttcgcatt accggcctgg cattttccgt ttatggcgcg gccaagatgc
     5821 cgggtcagcc gatcggcggc tggcttgcgg accggtttga accgcggatc gtgctcatcg
     5881 gcggtctcct gctcagcctg ccgacgatca tcatcatgga acgcttccag aacgtctggg
     5941 cctatatcct tgcctggatc atgttcggat tgaccctggc agtggtctgg ccggccgtct
     6001 acgcgatcgt gggtcaccga ttccgtcctg atcggcaagg gcgattactc gcgctggtca
     6061 gcatgggaca aattgccggc acagcggccg gcaccggtgc aggggctttt gtcgtggatc
     6121 acgtgtcgta tggcggagct tttgggctgg cgctgttgat agaagcaatt gcgctgtcga
     6181 tcgccattct cgccgttcca cgcacgagag tagacggccc cacctcgttg gttccaggta
     6241 ccgccctacc gccttccgga ttcagttcga tacggaggct ggtcacgctc aatgttgcca
     6301 tgctgatcgg gttgatcatg ctgctcagta cggccgtcgc catgatcgca cctgacctca
     6361 aaccgtacag cgaccagatc ttgcacgtca gctactcgac gtttgtgttg atgctggcta
     6421 tcccggcgac tgtggctgcg ctgctgatcg tgccatccgg atatattgcc gaccgcttcg
     6481 gccgaaacct accgattggg cttggtcttt tcctcttcgc tgccagcctg ttattgctaa
     6541 cgctgactca ctcgccactg ctatccacgc tgttcgctac catcgcggcg gtcggctatg
     6601 tactcgtttt gccggcattc agcgcctcac tgctcgacct ctcgtcaagc ggcaatcgcg
     6661 gtcttttgac cggcatcagc acgtcgatcc aggcaatcgg actcgtagtc ggtccagcag
     6721 tcggcggctt tctgatcgac gcctcagggc cgctggctcc gtttcgcgtc gccttcgcga
     6781 tcgtgttcgt caccggcctg ctctcgatcg tctacgccgg gcggacccgt gccatctatc
     6841 gcgagcgagt gcagagggcg ggaggtgcgt gacctacgtc gcctgcgtga gctaacagct
     6901 ttgcggcgtg tgaagcggtg gtagggccag gaagaggacg accggtcgaa ttacagctcg
     6961 aggtcttcgc tcaggttccg aagccggcga gataccggtt taccggcacc cagcgtgaac
     7021 aattccgtcg tgagcgttcg ttgcacgagt ccgtttggaa agagcgtatt cgggtcgccg
     7081 tcgcgtagct ggctacggtg gcaggcgatg gcggccagct tacggtcgga aaattcccgg
     7141 atgtcgattg ccgcggcaac gtcctgcggc tcaaccccaa gtttgcccgg atccagtccg
     7201 cgaatcgtgg ttgcatactc cgactccagc atgtgatcga acatcgggcg gaacattccg
     7261 cgcggaaatg ccgccaagta cagccgctgc ggagcccagc ccggaccgaa ttcggactcc
     7321 ggaaagcgat caccgccccc ggcggcgcca aacgccgcga ccaccagccg gtataacgcc
     7381 agatggtccg gatggccgta gactccgtcg gccccgagcg taatgacgac ctgcgggcgg
     7441 accgatcgga tttcgcggat gatctccccg gcgacgtcgg gaaagttcac gtcgccgaca
     7501 cccccatcgg gcaggccaag aaatgcaggc ggcggcaagc aaagcgcgtc gcaggcgcag
     7561 gcaagctcgg cggctcgtgt agccccgagg tcgttcacgg gggcgacgtt cgcacgcagg
     7621 tccatgcctg cactccccgc ggttgcgcag aggacgtgca cgccggctcc ttccgccgca
     7681 attctcgcga gtgtgccgcc gctgctgtat gactcgtcgt cggggtgtgg gaatatgccg
     7741 agcacacgca gcggttctgt cattgtggct tcactcgccc aaaccttcgt agggggcgac
     7801 gatgatctcc tgcgccctac cacgttgcag cgccaggctc agtagaaaag cgacgccaag
     7861 cgctagcgcc gcaacgagaa aggtttcgtc gaatgtgcgg tgcatgatcc cctggaatcg
     7921 cgcctggtag ttcgggtcgt cgaacgatat atccgccagt ctgcgcgtaa aggtccccag
     7981 cccctgggtt gccagcagcg ccagcccgac gatcatgccc gtcgtccaga cgacgagcaa
     8041 cagcgccgac gccgtcgcgc gctcgcgatg agagaccgcg tccacaaccg tcgcgacgat
     8101 cgggccatcg cacagaccga ggccgaggcc gatcgctccc agggcgcccc agaggtgcca
     8161 cgtcgagggc gcaagcgccg cggcctcccc aggcacgaac ggccaggcgc gcatccagat
     8221 gaatccggca atggcgaacc ccaggccgag cagcacagtc ggcctgttcc ccaccattgt
     8281 tgccagccaa ccaccaacca gaccgccgat cggcagcagc aatagaagcc gcatcaggtt
     8341 caggccgcct tgcgtcgtgc tgccgccgta caggacatcg gtcatcaagg gaacgttcac
     8401 aagcgcagtg atcagcgctc cgccgatgag gaagtacgtc agcatcgccg ccgagacgtg
     8461 gcgcacgcgt aagatgcgca ggtcaaccat cggtcgcggg ttcagccact cggcgcgcgc
     8521 aaatagcgcc agcgcgattg ctccggcagt tccgacagcc aggctgactg gcagcggtcg
     8581 cgggttggcg ccatcgtcgg tgagcgcgaa ggtcagtgcc gacagcccgg cgaccaaaag
     8641 tacagcggcg cgccaatcga tgcgctgacg ctcacccgtg cggtggtcgc gggccagcaa
     8701 tggcattgcg accatgaacg ggaggagcag cggcacgttc aaccagaaga tgactcgcca
     8761 gttccatgcg ccagagattg ccgcccccca aactggtccc agaaacgtgc tgccgttgtt
     8821 tgccgccgcc aatgcgccga gaacgagtgt ccgccgtttg gtactggtgg catccgcggc
     8881 tatcgcgagc gcgatcggca acagaccacc cccaccgatc gcctgcaacg cccgtgcggc
     8941 caccaaaaac gccaggtttg gcgcaaccgc caccatgagt gagccggcca cgaaaatcgc
     9001 gatcgcgaca aggaaaatgc gcacgcgtcc gaatacatcc gcaatgcggc cgacgatcgg
     9061 catcgaaacc gtatagccga tcaggtagcc gttgacgatc cacgatgcct cgccgaactt
     9121 cgtattgatc gggagacgaa tgtccttgat gatgtcgggc aaaaccgtga cgacgacggt
     9181 ttgatcgagc gttccaaaga acac
//
LOCUS       DATCPE010000081         5402 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U7219
            k141_210944, whole genome shotgun sequence.
ACCESSION   DATCPE010000081 DATCPE010000000
VERSION     DATCPE010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955033
            Sequence Read Archive: SRR1586279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 5402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 16:49:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 640
            CDSs (total)                      :: 631
            Genes (coding)                    :: 627
            CDSs (with protein)               :: 627
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5402
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210944"
                     /isolate="SMAG_U7219"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..610)
                     /locus_tag="VIM45_02480"
     CDS             complement(<1..610)
                     /locus_tag="VIM45_02480"
                     /inference="COORDINATES: protein motif:HMM:NF012421.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stilbene synthase"
                     /protein_id="HEY8820184.1"
                     /translation="MARIRSIGTAVPPYHLDSAAFKDALDRAFGRHFSDVRPILRMAE
                     RCGVQSRFIAVPPDEILAPRTLTDRNRRYGECAVELGERAARQALERSEVSPAEIDLI
                     VTVSCTGYMLPSLDAHLAGRLGLRPDVRRLPITEMGCLAGVAAFARAADLISGRRGQK
                     ALIVAAEFPSLTFQPADDSMDNLVSTLVFADGVGAAVLSEDDS"
     gene            821..1954
                     /gene="solA"
                     /locus_tag="VIM45_02485"
     CDS             821..1954
                     /gene="solA"
                     /locus_tag="VIM45_02485"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018541029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HEY8820185.1"
                     /translation="MSETYDAIVLGLGGMGSSAAYHLARRGKRVLGLEQFTPANDRGS
                     SHGRSRIIRQAYAEGAMYVPLLLRAYELWRELEMETGRVLMQITGGLMIGDPSSKTVS
                     GALASARQYGLAHELLDSTDVRRRFPAFHVDREVALYEAVAGALFPEDCVTAHIEQAA
                     ASGAELHFETPATAWRAIDDGVEVEAGGQTYRAERLIVTAGAWAGQILAGLSLPLLPE
                     RNLIYWFVPAANPEQFAPGRFPIFIWDGPGFPLYGLPDLRGDGPKAGIHHSGVTVDPS
                     DLCRDVHEYEVAAIRQRLQECLPAMNGSLRSAAVCMYTNTPDEHFAIGVHPTLPQVSI
                     AAGFSGHGFKFASVAGEVMADLATDGQTRHPITPFALGRFTDD"
     gene            2164..3558
                     /locus_tag="VIM45_02490"
     CDS             2164..3558
                     /locus_tag="VIM45_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase"
                     /protein_id="HEY8820186.1"
                     /translation="MEKPRTVGELRSSGYKVLPVREEMRKNLIEKIKKNEVLFPGIIG
                     YEESVVPSIENAILAGQDIIFLGERGQAKSRMIRALVSLLDEEVPVLTGCEIPENAFD
                     PITKTGRDIIAEQGDDAPISWVRREDRYGEKLATPDITIADLIGEVDPIKVAEGRYLS
                     DELTIHYGLIPRTNRGIFCINELPDLAERIQVGLLNIMEERDVQIRGYRIRLPLDVVV
                     LATANPEDYTNRGRIITPLKDRYGAQIRTHYPRNVDHEIDIMEREMWQFPVENYRVTV
                     PQYMKEIVAEITRLARRSPDVNQRSGVSVRASIADYESLLANALRRAIRLDEEAVVPR
                     ISDLPYIVPAISGKIELETVEDGREDQIIDKLIQGGVVSVFNRNFNIGELEEVVTRFK
                     AGLSLEVSDLMPSDEYIRIAKQIDALGKAVDKLGAGNDPAAVASAVEFVLEGLHLNKR
                     LNKDKVAGRAQYRG"
     gene            3571..>5402
                     /locus_tag="VIM45_02495"
     CDS             3571..>5402
                     /locus_tag="VIM45_02495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY8820187.1"
                     /translation="MLRYRYSEWDGTQEIPELAPDDILENLTDDLMNFGDLQHALRNM
                     LQRGMRNPGQQRTQGLRDLLQQLRQQRRQTLDKYNLSSVFDDLKKKLNEIVQRERDTI
                     DRRMQDANEGGEQQNGQPQAQGPQDGQTKSGGEQGQGQQAGQDGEQGEGQEGQEGGQS
                     GMQQSGTQGQQGSGQRTRGQQSGQRSGRQSSQQSGQQSSQRGQQGGEQGGQEGQAAGV
                     NDEFKQMLRNIAGRKQSFLENLPSDLGGAVKELQNYEFMDPEAQAQFQELMESLKKAM
                     METFFKDIYKQIQNMSPEDMARMKQMVRDLNKMLQDKMAGGQPDFDQFMQQYGDLFGD
                     NPPQSLEDLISQMQGQISQMQNLLDSMPGEMRRQLQDLLSDKIGDPELQSELSELGAN
                     LEYMFPMRDMRNQYAFRGDEELDLNEAMQLMDRMQGMDDLERQLERTQYGGDIDEIDE
                     ERLRELLGDEAAETLKQLKQFLEILENAGYIRKKGNTWELTPRGTRKIGQKALGEIYA
                     QLKKDSFGKHATRERGTGGERSDDTKKYEFGDPFHLNLEKTIFNSFYRETPKTPVRLH
                     PDDFEVFRSELLTQTATVMMLDLSWSMALRGSFQAAKKVALALN"
BASE COUNT         1181 a         1642 c         1714 g          865 t
ORIGIN      
        1 gactgtcgtc ttcggacagc accgcggccc cgactccgtc ggcgaagacc agtgttgaga
       61 caaggttatc cattgagtcg tcggcgggct gaaaagtgag cgaagggaac tcggccgcta
      121 ctatcagagc cttttggccg cgccggccgg atatgaggtc cgcggcgcgc gcgaacgcgg
      181 ccaccccggc gaggcagccc atctccgtga ttgggagccg tcgcacgtcc ggccgcaacc
      241 cgagccttcc ggcgagatgc gcgtcaagcg atggaagcat gtagccggta cacgaaacgg
      301 tgacaatgag gtcaatctcg gcgggactga cttcgctgcg ctctaacgcc tgccgcgcgg
      361 cgcgttcgcc aagctccacg gcgcactcgc cgtagcgacg gtttcgatcg gtaagagtgc
      421 gcggcgcgag gatttcatcg ggcggcacgg cgatgaagcg ggactgaacg ccgcaacgct
      481 cggccatgcg caaaatcgga cgaacgtcgg aaaaatggcg gccgaaggcg cggtccagcg
      541 catctttaaa tgccgcgctg tcgaggtggt acggcggtac tgccgtgccg atggaacgga
      601 tgcgggccat aaactctgca ccaggccggg gagggctccg tgaaagacaa atagcgagcg
      661 ggcgctagcg atacggtagc acaggccatc tgcgccgaac ggcagggcgc cgcattgacc
      721 atcgtacccg accgggagac cattgtcggc gtacggcgcg ccgagtgaaa ttcccagtcc
      781 gttgtaagca atggccgtat gccgcagccg ggagacgagc atgtccgaaa cgtacgatgc
      841 catcgtcctc ggcctcggcg gcatgggtag cagcgccgcg tatcacctgg cgcgccgagg
      901 caagcgcgtg ctcggtctgg agcagttcac tcccgccaac gaccgcggct cgtcgcacgg
      961 caggtcccgc attatccgcc aggcctacgc cgagggggca atgtatgtgc cgctcctttt
     1021 gcgcgcctac gagctctggc gcgagcttga aatggaaacc ggccgcgttc tcatgcagat
     1081 caccggcggc ttgatgatcg gcgatccgag cagcaagacg gtctccggcg cgcttgcgag
     1141 cgcccgccaa tacggcttgg cgcacgagct actcgactcg actgacgtgc ggcgccggtt
     1201 cccggcgttc cacgtggacc gggaagttgc gctgtatgag gcggttgccg gcgcattatt
     1261 tccggaggac tgcgtcaccg cgcatatcga acaggctgcg gcgagcgggg ccgagcttca
     1321 cttcgagact cctgccaccg cctggcgtgc aatcgatgac ggtgtcgaag tcgaagcggg
     1381 cggacaaacg tatcgagccg agcggttgat tgtcacggcg ggggcatggg caggtcaaat
     1441 ccttgccggt ctgagcctgc cgctactgcc cgaacggaac ctgatttact ggttcgtacc
     1501 ggccgcaaac ccagagcagt ttgcacccgg ccggttcccg attttcatct gggacggccc
     1561 tggctttccg ctctacggcc tccccgacct ccgcggcgac ggaccaaagg caggaatcca
     1621 ccatagcggc gtgacagtcg atccatccga tctctgccgc gacgttcacg agtacgaggt
     1681 cgccgcgatt cggcagcggc tgcaagaatg cctccctgcc atgaacggta gcctgcggtc
     1741 agcggcggtc tgcatgtaca ccaacacgcc tgacgagcat ttcgctatcg gcgtgcatcc
     1801 aaccctgcca caggtgtcga tcgccgcggg cttctccggt cacggcttca aattcgccag
     1861 cgtcgccggc gaagtcatgg ccgacctcgc cacagacgga caaaccaggc atccgattac
     1921 tccgtttgct ctagggcgct tcaccgatga ttgagaggcc gcaaagcgcg gggtatcatg
     1981 cgcgcaccac gtccaatcgc ctgtcgccgg tcgggggagg cgggaacaga catatgggga
     2041 ggcggacgtt tgggtggggc ggatggtccc ggggcgccgc cccgacggcg ccgaagcggt
     2101 atactgaagc cgtctagctg accggcgtta cttccagaga aaaatgaggt cgcagtggca
     2161 acaatggaga aaccccgcac cgtaggcgag ctccgaagct cgggctacaa ggttctgccg
     2221 gtccgcgagg agatgcgcaa gaacctgatc gagaagatca aaaagaacga agtgctgttt
     2281 ccgggcatca tcggctacga ggagtcggtg gttccgtcga tcgaaaacgc catcttggcc
     2341 ggccaggaca tcatttttct cggcgaacgc ggccaggcca aatcgcggat gatccgggcg
     2401 cttgtcagtc ttctcgacga agaggttccc gttcttaccg gttgcgagat tccggaaaat
     2461 gcctttgatc cgatcaccaa gaccggtcgc gacatcatcg ccgagcaagg cgacgacgct
     2521 cctatttcgt gggtacgccg cgaggaccgc tacggcgaaa agctggccac gccggatatc
     2581 accatcgccg acctgattgg cgaggtggac ccgatcaagg tggccgaggg acgatatctc
     2641 tccgacgagc tgacgattca ctatgggttg atccctcgca caaatcgcgg catattctgc
     2701 atcaacgagc ttcccgacct cgccgaaaga atccaggtcg gtctcctcaa catcatggag
     2761 gagcgcgacg ttcagatccg tgggtaccgt atccgtttgc ccctggacgt ggtcgtcctc
     2821 gccaccgcca acccagagga ctacaccaac cgcggccgca tcatcacacc gctcaaagac
     2881 cgttacggtg cccaaatccg tacgcattac ccacgcaacg tcgaccacga gatcgacatc
     2941 atggaacggg agatgtggca gttcccggta gagaactacc gggttacagt cccacagtac
     3001 atgaaagaga tcgtggccga gatcacgcgc ctggcgcgcc gcagtccgga cgtcaaccag
     3061 cggtccggcg tctcggtacg cgcttcaatc gcggactatg aaagcctgct ggccaacgcg
     3121 ctgcgacgcg cgatccggct ggatgaagag gctgttgtcc cacgcatcag tgacctgccg
     3181 tacatcgtgc cggcgataag cggcaaaatc gagctggaga ccgtcgaaga cggccgcgaa
     3241 gaccagatta tcgacaagct gatccagggc ggcgtcgtgt cggtgttcaa ccgcaacttc
     3301 aatatcggcg agctcgaaga ggtcgttacc cgtttcaagg ccggcctgtc gctggaagtc
     3361 tccgatttga tgccgtcgga tgagtatatt cgcatcgcca agcagatcga cgcactgggc
     3421 aaggccgtcg acaagttagg cgcgggaaac gatccggccg cggtggcttc agccgtcgaa
     3481 tttgtgctcg aagggctgca cttgaacaag cgcttgaaca aggacaaggt cgccggccgc
     3541 gctcagtacc gcggctagga cggaagacgc atgcttcgtt atcgctattc cgaatgggac
     3601 ggcacccagg agattccgga gcttgcgccg gacgatatcc tcgagaacct gaccgacgac
     3661 ctgatgaact tcggcgatct gcagcatgcc ctgcgcaaca tgctgcagcg cggcatgcgt
     3721 aaccccggac agcagcgcac gcaggggctg cgcgatctcc ttcaacagtt acgacagcag
     3781 cgccgccaga cgctcgacaa atacaacctg agctcggtgt ttgacgacct caagaagaag
     3841 ctgaacgaga ttgtccagcg agagcgcgat acgatcgacc gtcgcatgca ggacgcaaac
     3901 gagggcggtg agcagcaaaa cggccagccg caggcgcagg gaccacagga cggtcagacg
     3961 aaatccggag gcgagcaggg gcagggtcaa caggcgggcc aggacggcga gcagggcgaa
     4021 ggacaggagg gtcaggaggg cggtcagtcc ggcatgcagc aatcgggcac gcagggacag
     4081 caaggctcgg gtcagcggac gcgaggacag caatccggcc agcggtccgg ccgacagtcg
     4141 tctcagcagt ctggccagca atcgtctcag cgcggacagc agggcggaga gcagggcggc
     4201 caggagggtc aggccgctgg cgtgaacgac gagttcaagc agatgctccg caatatcgcg
     4261 gggcgcaagc agagcttcct ggaaaacctg ccgagcgacc tcggcggtgc cgttaaggag
     4321 ctgcagaact acgagttcat ggaccccgag gcccaggccc agtttcagga gctgatggaa
     4381 tccttgaaga aggcgatgat ggagacgttc ttcaaggaca tctacaagca gatccagaac
     4441 atgtcccctg aggacatggc gcgcatgaaa cagatggtgc gcgacctcaa taagatgctg
     4501 caggacaaga tggcgggcgg tcagcccgac ttcgaccagt tcatgcagca atacggcgac
     4561 ctgtttggcg acaacccgcc gcaaagcctc gaagacctga tatcgcaaat gcaggggcag
     4621 atctcacaga tgcaaaatct gctcgacagc atgccggggg agatgcggcg gcaactacaa
     4681 gatctgctga gcgacaagat cggcgacccg gagctgcaat cggagctgtc cgagctcggc
     4741 gccaacctcg agtacatgtt cccaatgcgc gacatgcgca accagtacgc gttccgcggc
     4801 gacgaagagc tcgatctcaa cgaggcgatg cagctcatgg accgtatgca ggggatggac
     4861 gacctcgagc gccagctcga gcgcacccag tatggcggcg atatcgacga aatcgacgag
     4921 gagcggctgc gcgaattgct cggcgacgag gctgccgaaa cactcaaaca gctcaagcag
     4981 ttcctggaga tcctcgaaaa cgcgggctac atccgcaaga aggggaacac gtgggagctg
     5041 accccgcgcg gcacacgcaa gatcggccag aaagcgctgg gcgagatcta cgcgcaactc
     5101 aagaaggaca gcttcggcaa gcacgccacg cgcgaacgcg gcacaggcgg agagcggagc
     5161 gacgacacca agaagtacga gtttggcgat cccttccacc tcaacctgga aaaaacgatc
     5221 ttcaactcgt tctaccgcga gacgccaaaa actcctgtgc gcctgcaccc cgacgacttc
     5281 gaagtctttc gatcggagct gctgacgcaa acagctaccg tgatgatgct cgacctctca
     5341 tggtcgatgg cgctgcgcgg cagcttccag gcggccaaga aggtcgcgct ggcgctcaac
     5401 aa
//
LOCUS       DATCPE010000082         5005 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U7219
            k141_178850, whole genome shotgun sequence.
ACCESSION   DATCPE010000082 DATCPE010000000
VERSION     DATCPE010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955033
            Sequence Read Archive: SRR1586279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 5005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 16:49:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 640
            CDSs (total)                      :: 631
            Genes (coding)                    :: 627
            CDSs (with protein)               :: 627
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5005
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178850"
                     /isolate="SMAG_U7219"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..735
                     /locus_tag="VIM45_02500"
     CDS             <1..735
                     /locus_tag="VIM45_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012882438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEY8820188.1"
                     /translation="TLGIETLGGVMTPLIQRNTTIPTSKSQVFSTAADGQSSVEIHVL
                     QGERAMAADNKSLGRFILDGILPAPRGVPQVEVTFDLDANGIVNVSAKDKGTGKEQKI
                     TITASSGLSKAEVEKLQREAEMHADEDRKRREEIEMRNNADNLAHQAEKLLRENGDKI
                     PVELKSEVEGKAETLRNALKGDDADAIRRDFESLSEALQKVGAAIYGAAGSGAGAGFG
                     EDGQSANGHAEGKPEEGTVEGEFREV"
     gene            784..1902
                     /gene="dnaJ"
                     /locus_tag="VIM45_02505"
     CDS             784..1902
                     /gene="dnaJ"
                     /locus_tag="VIM45_02505"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEY8820189.1"
                     /translation="MATQQKDLYEVLGVPRTATAEEIKKAFRKLAMQYHPDRNSDSSA
                     EERFKEINTAFEVLSDPEKRAAYDRFGHAGIGQQGPSGFEGFSNFGGFGDIFDAFFGG
                     ATRQRRGPARGPDLRFNLTLKFEEAVFGAEKEIEVSRTEQCAMCGGSGAEPGSKPERC
                     PSCNGTGEIRRVQQSIFGQFVNVTQCDRCHGEGRIITSLCAQCRGAGRERKVRKLQVT
                     VPPGVDNGQQLRLSAEGEAGVQGGPPGNLYVLLHVQEHDVFKRDEDDIILDLPVNVSQ
                     ATLGDEVQIPTIDGKAQLKIPAGTQSGRIFELKGNGVPHLRGGGRGDQIVRVRVVTPT
                     NLTPEQKRLVSELGKSLGTATMPHEEKGFFDRIKDAFG"
     gene            1914..2828
                     /gene="prmA"
                     /locus_tag="VIM45_02510"
     CDS             1914..2828
                     /gene="prmA"
                     /locus_tag="VIM45_02510"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEY8820190.1"
                     /translation="MDWVELSAEVSGDRVEDLCSALENVAPGGCSIEDPIVPLGPEEG
                     VRREPWRPSTVRIYLPVDVHIDERRRDAAVAAARQSVRFRERPVREADWANAWKEFFQ
                     VEQIGRHIVVRPTWREFSPSPEDIVLHLDPGMAFGTGQHETTRMCLVVLEELAEPGMR
                     VLDVGCGSGILSVAAARLGAASVTAVDLDSVAVEATAVNAARNGVAAIIRVAEGSLDD
                     GWPFGAMPSAEFDLVLANIHAAAAETLAPAYWHALRPGGTLVASGMIAERLDAACAAL
                     SNNGFDRLDIRVLGEWRTVVARRPDASG"
     gene            2836..3618
                     /locus_tag="VIM45_02515"
     CDS             2836..3618
                     /locus_tag="VIM45_02515"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HEY8820191.1"
                     /translation="MHRFHVPDLRLDNRAAIMTPTQSRQIARVLRMRSGEQIELFDGR
                     GDLAEAKLTEVNANRVSATILSTSRVAWPLPWRTTLYLAVIRPQRFEWAIEKATELGA
                     ASIVPVFCSNTTHGGDSVGSSRHGRWQQIAIEAAEQCGSAFVPRVGAAVTLQEALAEA
                     ASLRIFAGESAGEKKGERLMDVIAGIRRRDGDDPLSVGLYIGPEGGFAAAEVETSMAA
                     GCRLVTLGPRILRAETAAVAALSLMLAGASNSDQMPSSLSII"
     gene            3735..4763
                     /locus_tag="VIM45_02520"
     CDS             3735..4763
                     /locus_tag="VIM45_02520"
                     /inference="COORDINATES: protein motif:HMM:NF014234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HEY8820192.1"
                     /translation="MAVEKVTIIGTGLIGSSIGLALKAAKLPNVQIVGHDIEPDAAKA
                     AQKRGAVDSVQSHLEGAVADARLIILATPPLAIRPLLEDLAELAPEGSIITDVSSTKA
                     DIMRWAKELLPDNVSFVGGHPMAGKEETGAQAADADLFRGKAYAVVPSTSATEGAVKS
                     VLGLVSIVGADPVFIDAEEHDQYVAAISHMPMVASTAIFTLIRNSQGWNEISPLASTG
                     FKDMTRLASGDPYMNHDICVTNADGVVHWLDRFIAELLRYKNMVLDNHEELFNTFNAA
                     QLQREAFLNGTDRPAASRVELPSTGEQINSMLFGGLLAQGAKKYEKMMKRVDRNDLRR
                     GINDDDDE"
     gene            4851..>5005
                     /locus_tag="VIM45_02525"
     CDS             4851..>5005
                     /locus_tag="VIM45_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010935846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular matrix/biofilm biosynthesis
                     regulator RemA family protein"
                     /protein_id="HEY8820193.1"
                     /translation="MATELIHVGFGNVLAVNRVIAIVAPNSAPTKRLIQEGKNKGLTV
                     DMTSGRRT"
BASE COUNT         1038 a         1516 c         1598 g          853 t
ORIGIN      
        1 acgctgggca ttgagacgct tggcggcgtg atgacgccgc tgattcagcg caacacaacg
       61 atcccgacca gtaagagcca ggtgttctcc actgcggccg atggccagtc gagcgtggag
      121 atccacgtcc tgcagggcga acgcgcgatg gcggccgaca acaagtcgct cggccgcttt
      181 atcctcgacg gcatcctgcc tgcgccgcgc ggcgtacccc aggtcgaagt tactttcgac
      241 ctggacgcga acggcatcgt gaacgtctcg gccaaggaca agggcaccgg taaggagcag
      301 aagatcacga tcaccgcttc gtccggcctc tcgaaagcag aggtcgagaa gctgcagcgc
      361 gaagccgaga tgcacgcgga tgaagaccgc aaacgtcgcg aagagatcga aatgcgcaac
      421 aacgccgaca acctggcgca ccaggcggag aagttactgc gtgaaaacgg cgacaagatc
      481 ccggttgagt tgaagagcga ggtcgaaggc aaggcagaga ccctgcgcaa cgcactcaaa
      541 ggcgacgacg ctgatgcaat tcggcgtgac ttcgagtcgc tttcggaagc tcttcagaaa
      601 gtcggcgcgg ctatctatgg cgcggcgggc tcgggggccg gagccggctt cggagaagac
      661 ggtcagagcg ctaacggcca cgccgaagga aagcccgagg aaggcaccgt cgagggcgaa
      721 ttccgcgagg tctagatcgt cggaaggatg tccgaccccg accaaggatg gataaagaga
      781 aagatggcca cacagcagaa agacctctac gaggtgttgg gcgttccccg caccgcgaca
      841 gccgaagaga tcaagaaggc ctttcgcaag ctggccatgc agtaccaccc cgatcgcaac
      901 agcgattcga gtgccgagga gcgattcaag gagatcaata ctgcctttga agttctatcc
      961 gatccggaga aacgggccgc gtacgatcgc tttggtcacg cgggcatcgg ccagcaggga
     1021 ccgagcggct tcgaggggtt cagtaacttc ggtggcttcg gcgatatctt cgacgccttc
     1081 ttcgggggcg ctacgcgtca gcgacgcgga ccggcgcgcg ggcccgatct gcgcttcaac
     1141 ctgaccctca agttcgaaga ggccgtcttc ggcgcagaga aggagatcga ggtcagccgc
     1201 acagagcagt gcgcgatgtg cggcggcagc ggcgcggagc caggcagcaa gccggaacga
     1261 tgccctagct gcaacggcac gggcgaaatc cgccgcgttc aacagagcat tttcggtcag
     1321 tttgtcaacg tcactcagtg cgaccgttgt catggcgaag gccgaataat cacgagcctc
     1381 tgcgcccagt gccggggggc aggacgcgag cgcaaagtac gcaagctgca ggtcaccgtt
     1441 cctcccggag tggacaacgg ccagcagctt cggctctccg ccgaaggcga agcgggcgtg
     1501 caagggggac cacccggaaa cctgtacgtg ctcttacacg ttcaagagca cgacgtgttc
     1561 aagcgggacg aggacgacat tatcctcgac ctgccggtca acgtctccca ggcaacgctc
     1621 ggagacgagg ttcagatccc aaccatcgac ggcaaggcgc agttgaagat cccggccgga
     1681 acgcagtcgg gccgaatatt cgagttgaag ggcaatggtg tcccccacct gcgcggcggc
     1741 ggacgcggtg accagatcgt gcgcgtccgc gtagtcactc ccaccaacct caccccggaa
     1801 cagaagcgcc tcgtcagcga acttggtaaa tcactcggta cagccacgat gccgcacgaa
     1861 gagaaaggct tcttcgatcg catcaaggac gcattcgggt aagcggggcc ctattggact
     1921 gggttgaact ctccgcggag gtgtcgggcg accgggtcga ggatctgtgc tcggcgctcg
     1981 agaatgtagc gccgggcggc tgcagtatcg aagaccctat cgtgccgctt ggccccgagg
     2041 aaggcgtccg acgcgagccc tggcgcccgt cgacagtccg aatctacctg cccgtggacg
     2101 tacacatcga tgaaagacgt cgcgacgccg cggtggccgc cgcgcggcaa tcggtacgct
     2161 tcagggagcg gccggtgcgc gaggctgatt gggcgaacgc ctggaaagag ttcttccagg
     2221 ttgaacagat cggccggcac attgtggtgc gtccaacgtg gcgagaattc tccccgagcc
     2281 ccgaagacat cgtgctgcac ctcgatcccg gcatggcctt cggcacgggg caacacgaaa
     2341 ccacccgcat gtgcctcgta gtgcttgaag agttggccga accgggaatg cgcgtcctcg
     2401 atgtgggatg cggatccggt atcctctctg ttgcggcggc cagacttggt gcggcctccg
     2461 tcaccgcggt ggacctcgat tccgtggccg tcgaggcaac cgccgtcaat gcagcccgca
     2521 atggcgtggc tgctattatt cgagtagccg aggggagcct ggatgacggt tggcctttcg
     2581 gagctatgcc cagcgctgaa ttcgacctcg tcctggccaa tattcatgcc gcggccgcgg
     2641 aaacgctggc gccggcctac tggcatgcac ttcgccccgg cggaacgctg gttgccagcg
     2701 gcatgattgc agagaggttg gatgccgcct gcgccgcact ttcgaacaac ggctttgatc
     2761 ggctcgacat ccgcgtcctc ggcgaatggc gcaccgtcgt cgctcggcgc cccgatgctt
     2821 caggttagtt gagccatgca ccgtttccac gtgccggacc ttcgattgga taatagagcg
     2881 gccatcatga cgccgacgca gtcgcggcag atcgcccggg ttctgcgcat gaggtccgga
     2941 gagcagatcg agttgttcga cggtcgcggc gatcttgcgg aggcgaagct taccgaggtc
     3001 aacgccaacc gggtatccgc gacgatccta tccaccagcc gtgtcgcctg gcctcttccc
     3061 tggcgcacga cgctctactt ggcggtcatc cggccgcaac ggtttgagtg ggcgatcgaa
     3121 aaggcgaccg aattaggtgc ggcgtcaatc gtacctgtct tctgttcgaa taccacccac
     3181 gggggcgatt cggtcgggtc gtcgcggcac ggacggtggc agcagatcgc cattgaggcg
     3241 gcggaacagt gcggctcggc attcgtgccc cgcgtgggcg cggccgtgac tctgcaagaa
     3301 gccttggctg aagccgcgag ccttcgcatc ttcgccgggg aaagcgctgg agaaaagaag
     3361 ggcgagcgcc tgatggatgt gatcgccgga attcgtcgtc gagacggcga cgacccgtta
     3421 tccgtcggcc tctacatcgg gccagaaggc ggattcgcgg cggccgaagt ggagacgtcc
     3481 atggcggcag gttgcagact ggtgacgctt ggccctcgta tcctgcgcgc cgaaactgct
     3541 gcggtcgcgg cgctgtcgct gatgctggca ggcgcctcca atagcgatca aatgccctcg
     3601 tctctttcca tcatctgagc cgtcgactcg ggatttttca cgctatttgc tacgccaacg
     3661 tgtcgcgtgg tgctatcgta tgctacaggc ggaattcgac gtagcgaatt ccgcgtgtgc
     3721 ggtaagggcg gtttatggca gtcgaaaagg tcacaattat cggcaccggt ttgatcggga
     3781 gctcgatcgg cctcgcactg aaggcggcga aactccctaa tgtccagatc gtgggccacg
     3841 acatcgagcc tgatgcggca aaggcggcgc aaaagcgcgg tgccgtagac tctgtccaat
     3901 cgcacttaga aggggccgtc gcagacgctc gccttatcat cctggccaca ccgccgctcg
     3961 ccatccggcc gctgcttgaa gacctggccg agcttgctcc cgaaggctcg atcatcaccg
     4021 acgtcagcag cacaaaggcc gacatcatgc gttgggcaaa agagctgttg ccggacaacg
     4081 tctccttcgt cggcggccac ccaatggccg gcaaggagga gacgggggca caagccgcgg
     4141 acgccgacct gtttcgcgga aaagcatatg ccgtcgttcc gtccacctcc gccactgaag
     4201 gcgcggtgaa gtctgtgctg ggtctggtct ccatcgtcgg cgccgatccc gtttttatcg
     4261 acgcggagga gcacgaccag tatgtggcgg caatcagtca catgccgatg gtcgcctcca
     4321 cggccatttt cacgctgatt cgcaacagcc agggttggaa cgaaatatcg ccattggcaa
     4381 gcaccggctt caaagatatg acgcgccttg cttcgggcga tccgtacatg aaccacgaca
     4441 tctgcgtcac caacgccgac ggggtggtac actggctcga ccgcttcatc gccgaattgc
     4501 tgcgctacaa gaacatggtg ctagacaatc acgaagagct gttcaacacg tttaacgccg
     4561 cacaactcca gcgcgaagcc ttcctgaacg gcaccgaccg gcccgcggcc agtcgcgtcg
     4621 aactgccgtc taccggcgaa cagatcaaca gtatgctgtt cggcggtctg ctggcgcagg
     4681 gcgccaagaa gtacgagaag atgatgaagc gcgtcgatcg gaacgatctg cggcgcggca
     4741 tcaatgacga tgacgacgaa tagcagcagc ttccgggtca aactccatca ccggcccttc
     4801 atatagcaag aagcgaccga cgacaggggc cgaacggccg gagggaacgc atggcaaccg
     4861 aactgattca tgttggattc gggaacgtcc tggcggtgaa ccgagtcatt gcgatcgtgg
     4921 cgccaaactc cgcgcccacg aagcggctca tccaggaagg caagaacaaa gggttgaccg
     4981 tcgacatgac cagcggccgc cgcac
//
LOCUS       DATCPE010000083         3158 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U7219
            k141_178888, whole genome shotgun sequence.
ACCESSION   DATCPE010000083 DATCPE010000000
VERSION     DATCPE010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955033
            Sequence Read Archive: SRR1586279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 16:49:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 640
            CDSs (total)                      :: 631
            Genes (coding)                    :: 627
            CDSs (with protein)               :: 627
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3158
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178888"
                     /isolate="SMAG_U7219"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1497
                     /locus_tag="VIM45_02530"
     CDS             <1..1497
                     /locus_tag="VIM45_02530"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEY8820194.1"
                     /translation="TADGAETDLDLGHYERFIDQDLTKASSVTTGQIYQAVISKERRG
                     DYLGGTIQAVPHLTNEIKARIRGVASETGAEVLIIEVGGTVGDIESQPFLEAIRQMRN
                     EEPREDILSIHVAFLPYIGASGELKTKPTQHSVRELRSMGVQPDVIVCRSDHPVSEEL
                     TAKVALFCDVEQRAVVPLLTADSIYRVPLILEDAGLGDYVIERLHLNAGSRDLAEWRT
                     LIGQMSDPERRLEVAIVGKYIQLHDAYLSVKESLIHAGIYHRSEVGIRWVQAEDLNEN
                     NLDATLSEVDGIIVPGGFGERGVEGKILAARYAREYKVPYLGLCLGMQVMVIEFARHM
                     WGNNEANSTEFAETRLPVIDLMPDQNGVSEKGGTMRLGGYVCRLLPGTRAREAYGTDE
                     VVERHRHRFEFNNAYRAILEKEGLIASGVSPDGRLVEISELRDHPWMVGSQFHPEFRS
                     RPNRPHPLFRDFIGAAVRKADSCPADQPAVSILKAATGAASVASATAS"
     gene            1500..1976
                     /locus_tag="VIM45_02535"
     CDS             1500..1976
                     /locus_tag="VIM45_02535"
                     /inference="COORDINATES: protein motif:HMM:NF021538.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2269 family protein"
                     /protein_id="HEY8820195.1"
                     /translation="MSFVNIWKFAHIASLFLLMAGIGAVMAPVYRAWHNKDLRTQMYA
                     FTIAADNETALLLPGALLTGVTGVGWVASGHHNVVHEGWLLTLTIVYLITTFICLPLL
                     GLGLRRARLASLKSWKSGKLTPELQSTLADNVPLVFGSLIIASLPLMGWLAVFKPF"
     gene            1993..>3158
                     /gene="rho"
                     /locus_tag="VIM45_02540"
     CDS             1993..>3158
                     /gene="rho"
                     /locus_tag="VIM45_02540"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012190910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEY8820196.1"
                     /translation="MNIAELELKSRDDLVTQALNIGVFNGAALPKQDLVQRLLSHQAD
                     QFGHIFGGGVLDIVDDGYGFLRGESLLPGVNDVYVSQTQIRRFGLRTGDYVTGQVRPP
                     KDSEKYYGMLRVESVNGIDPEVAKQRPYFEQLTAVFPNRMIDLETTPQNLSTRLINLI
                     SPVGRGQRGLIVSPPKAGKTMLLKAIANGITTNYSDIHLIVALIGERPEEVTDMKRSV
                     KGEVIASTFDEPVEDHTRVAEMSLERAKRLVESGKDVVILMDSITRLTRAYNLAMPPS
                     GRTLTGGIDPIALYPPKRFFGAARNTDEGGSLTIIATCLVDTGSRGDEVIYEEFKGTG
                     NMELILDRKLSERRIFPSIDISRSSTRRDDLLLGEAALGKVVLMRRMTQMISQNS"
BASE COUNT          657 a          939 c          968 g          594 t
ORIGIN      
        1 actgccgacg gcgccgagac cgatctcgac ctggggcact atgagcgctt catcgatcag
       61 gacctgacca aggcaagctc agtcactacc ggacagatat accaggccgt gatctccaaa
      121 gagcggcgtg gagactactt gggcggcacg atccaggctg tgccgcacct gacaaacgag
      181 atcaaggcac ggattcgcgg ggtcgcgagc gagacgggcg ccgaggtgct catcatcgag
      241 gtcggcggca cggtcggcga catcgagagc cagccatttc tcgaggcgat tcgccagatg
      301 cgcaacgagg agccgcggga ggatatcctc tcgatccacg tggcgttcct gccgtatatt
      361 ggcgcttccg gcgaattgaa gaccaaacca acgcagcaca gtgtgcgtga gctgcgcagc
      421 atgggagtcc agccggacgt catcgtttgc cgcagtgacc atccggtatc ggaagagttg
      481 accgccaaag tcgcgctgtt ctgcgacgtt gaacagcgtg ccgtggtgcc gttgttaact
      541 gctgactcga tttatcgcgt gccgcttatt ctcgaggacg cgggactggg cgattacgtg
      601 atcgagcgcc tacacctgaa cgcgggcagt cgcgatctcg ccgagtggcg gacgctgatc
      661 ggtcagatgt cagaccccga acgccggctc gaagttgcga tagtgggtaa gtatatacag
      721 ttgcatgatg cctacctgtc ggtgaaagaa tcgctcatcc acgcgggtat ctaccaccgt
      781 tccgaggtcg gcattcggtg ggtgcaggcg gaagacctca atgagaataa cctcgatgcg
      841 acactgtcgg aggtcgacgg catcatcgtt ccgggcggct ttggcgagcg cggtgtcgaa
      901 ggcaagatcc tcgccgcccg ctatgcccgt gagtataagg ttccatacct cggactctgc
      961 ctgggcatgc aggtgatggt gatcgagttc gcacggcaca tgtggggcaa taacgaggca
     1021 aactcaaccg agtttgcgga gacgcgcctg ccggtgatcg acctaatgcc cgatcagaac
     1081 ggcgtcagcg aaaagggcgg cacgatgcgg ctcggcggtt acgtttgccg cctgctaccg
     1141 ggaacacgcg cgcgcgaggc gtacggcacc gatgaggtgg ttgaacgcca ccgccatcgt
     1201 tttgagttca acaacgccta tagggccatt ctcgagaagg aaggcctgat cgcgagcggg
     1261 gtatcccctg acggccggct ggttgagatc agcgagttgc gtgaccaccc gtggatggtc
     1321 ggttcacagt tccatccgga gttccggtca cggccgaacc ggccgcaccc gctgttccga
     1381 gacttcattg gggcggccgt gcgcaaagct gactcgtgcc ccgctgatca gccggccgtt
     1441 tccatcctca aagcggcgac gggcgccgca tccgtggcga gcgcgacggc gagctagttg
     1501 tgtccttcgt aaacatctgg aagtttgcgc atatcgcttc gttgttcctg ctcatggccg
     1561 gtatcggggc agttatggcg ccggtctacc gcgcatggca caacaaggat cttcgcacgc
     1621 agatgtacgc attcacgatc gcggcggata acgagactgc gctcctgctg cccggggcct
     1681 tgctaaccgg cgttactgga gttggctggg tcgccagcgg tcaccataac gtcgtgcacg
     1741 agggctggct gctgaccctg accatcgtgt atctgatcac gacatttatc tgcctaccgt
     1801 tgctggggct cggcctgcgc agagcgcgac tcgcgtcgct aaaatcgtgg aaaagcggca
     1861 aactgacacc ggagctgcaa tcgacgctgg cagacaacgt gccgcttgta tttgggtcgt
     1921 tgatcatcgc ttccctgccg ctcatgggtt ggctggcggt gttcaagccg ttttaggagt
     1981 ggagacctca aagtgaatat cgcggaactc gaactcaaat cgcgcgacga tctcgtcact
     2041 caggcgctca atataggcgt gttcaacggc gcggcgctcc ctaaacagga cctcgtacaa
     2101 cgactgctct cccaccaggc cgaccagttc gggcatatct tcggtggcgg cgtgctggac
     2161 attgtcgacg acggctacgg cttcttgcgc ggagagtcac tcctgcccgg cgtcaacgac
     2221 gtatacgtct cgcagacgca gattcgccgt ttcgggctgc gcaccggcga ctacgtcacc
     2281 ggccaggtac ggccaccaaa ggacagcgaa aaatattacg gcatgctcag ggtggagagc
     2341 gtaaacggta tagatccaga ggtggcgaaa caacggcctt atttcgaaca gttaacggct
     2401 gtctttccga accgcatgat tgacctggag accacgccgc agaacctgtc cacgcggctc
     2461 ataaacctca tctccccggt cgggcgcggt caacgcggcc tcattgtctc gccccccaaa
     2521 gctggaaaga caatgctgct caaggcgatc gccaacggaa tcacaaccaa ttacagcgac
     2581 atccacctca tcgtcgcatt gatcggcgag cgacccgagg aagttaccga tatgaagcgg
     2641 tcggtcaaag gtgaagttat cgcttcgacc ttcgacgaac cggtcgaaga tcacacgcgc
     2701 gtagccgaaa tgtcgctgga gcgcgccaag cgactcgtag agagcggcaa agacgtggtc
     2761 atcctgatgg acagcatcac gcgcttgacc cgcgcctaca acctcgcaat gccgccgagc
     2821 ggtcgaacgt tgacgggcgg catcgacccg attgccctct acccgcccaa gcgcttcttc
     2881 ggcgccgcac gtaacaccga cgagggcggc agcctcacga ttattgccac ctgccttgtg
     2941 gacaccggca gccgcggtga cgaggtgatc tacgaggaat tcaaaggtac cggcaacatg
     3001 gagctgatac tggaccgcaa gctgtcggag cggcgaatct tcccgtcaat cgacatatcg
     3061 cgcagcagca cccgacgcga cgacctgctg ttgggcgaag ccgcgcttgg caaagtggtg
     3121 ctcatgcggc ggatgacgca gatgatcagt cagaactc
//
LOCUS       DATCPE010000084         2998 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U7219
            k141_568236, whole genome shotgun sequence.
ACCESSION   DATCPE010000084 DATCPE010000000
VERSION     DATCPE010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955033
            Sequence Read Archive: SRR1586279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 16:49:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 640
            CDSs (total)                      :: 631
            Genes (coding)                    :: 627
            CDSs (with protein)               :: 627
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2998
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_568236"
                     /isolate="SMAG_U7219"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(142..609)
                     /locus_tag="VIM45_02545"
     CDS             complement(142..609)
                     /locus_tag="VIM45_02545"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEY8820197.1"
                     /translation="MNLGLNPLTSPEARDNPYPFFAMARQMMPVLRLEQFGVWSAFLY
                     EDCRNILRDSKRFSSSFIRRMPEGQTRPRPSMLNSDPPRHTRLRELVNKAFTPRMVAQ
                     LEPRVQSIADELLDAVVVTGSMDLIDDLAYPLPVIVIAEILGVPPSDRAKFKR"
     gene            complement(649..1680)
                     /locus_tag="VIM45_02550"
     CDS             complement(649..1680)
                     /locus_tag="VIM45_02550"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY8820198.1"
                     /translation="MHSRYNEHTLDESGIVASEGPFMLLQHLETFVRVVEEGSFTRAG
                     ELLNVSQPAVTRQIAALERELGVPLVERGGRGLHLTTVGQTAYAHARHILAQVEHLRS
                     DVSSLSDPKRGEVSIACVTTVGLFTLPRLLADYRAAYPEVRLRVWSARADGVLDRLLE
                     GNADLGLTSTPVAHPRLISTPLFEDPVIPVAAPQFAAALPDPLPLEQLAELDMILFQA
                     PSRFRTHVDAELEQAGVYPRITMEFDSHEAVRTAAQMGYGIGIVPRQAVTAELAGGQL
                     VELHVAGLPEIKRTTCLVERRGDKHLPAAANFIKLLLERYGGAAGVARTRSPSSAPAL
                     RLAASTRQV"
     gene            1752..>2998
                     /locus_tag="VIM45_02555"
     CDS             1752..>2998
                     /locus_tag="VIM45_02555"
                     /inference="COORDINATES: protein motif:HMM:NF013707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HEY8820199.1"
                     /translation="MLREIVWKIGGEQGQGLDPTSDVFATVCNRMGYWVYAYKTFSSR
                     IKGGHTDFTVRVGQDRKLSPAPKLHMLVAMDQESIDILVEDLMGDGILLADSGFKPQA
                     PDGVNMITADLTKMARDLGNPVYRNMIAVGASAQVLGIPLEPFKEYVQEKFGRKGESV
                     VKGNWTALDQGAQIARDQVGDKGFRLGDPDGKSDRLLVTGNDAMTLGALAAGCRTIFA
                     YPITPASEVLEVGAKIFPKFGGVSVQMEDELGALGACVGAGFAGARALTCTSGPGISL
                     MQELMGLAGMTEVPVVIIDTQRGGPSTGMPTKQEQSDLFALIHGGHGEAPRIVLAPGT
                     GEQAFYDIALAFNLADNYHCPVILASDLGLALWQQTVDPFDVTKVVIDRGPIVEQSEL
                     DKIGRDGFERYADSETGVSARSFP"
BASE COUNT          564 a          906 c          978 g          550 t
ORIGIN      
        1 gatcagatcc tcgcgcggtt cctggcggcg ctgctcgatc acgcgggtga agtactcgtt
       61 cagctcctgg atcgtctccg ttggcgcccg gtcgggagtc gtgctgaagc cggcgccgag
      121 ggtggcgaca agcgtgtcgg atcaacgctt gaactttgcg cggtcggatg gcggcacgcc
      181 gagaatctcc gcgatcacga tgacaggcag cgggtaggcg agatcgtcga tcagatccat
      241 ggagccagtc acgaccaccg catcgagtaa ttcgtccgcg attgattgaa cgcgtggctc
      301 gagctgcgcc accatgcgcg gtgtgaacgc tttattcacg agctcgcgca gccgtgtgtg
      361 ccggggaggg tcggagttga gcatgctggg ccgcggcctt gtctggcctt ccggcatgcg
      421 gcggatgaac gacgaggaga agcgcttgga atcgcgcaga atgttgcgac agtcctcata
      481 caggaacgca ctccagacgc cgaactgttc gagccgcagc accggcatca tctgcctcgc
      541 catggcgaaa aacgggtatg gattatcgcg cgcctcaggc gaggtcagtg ggttcaggcc
      601 gaggttcatg accactgcgg atgcctcctg tgacggctta caccagcatc agacctgacg
      661 cgtgctcgcg gcaagccgca aagccggtgc ggaagaagga gatcgcgttc tagcgactcc
      721 ggcggcccca ccgtaccgtt ctaacagtag cttgatgaag ttcgcggcgg ccggcaggtg
      781 tttgtcgccc cgacgttcga ccaggcacgt cgttcgcttt atctcgggca atcccgctac
      841 gtgtagttcc accaactgcc cgccagctag ctcggctgtg accgcttggc ggggcacaat
      901 gccgatgcca tagcccattt gcgccgcggt acgtaccgct tcgtggctgt cgaactccat
      961 ggtgattcgg ggatacacgc ccgcctgttc caactcggcg tccacgtggg tacgaaaacg
     1021 tgaaggcgct tgaaaaagga tcatgtccaa ctcggcaagc tgttcgagcg gcaaggggtc
     1081 gggaagggcg gccgcaaact gcggagccgc caccgggata accgggtctt cgaacagggg
     1141 cgttgagatc agacgcgggt gcgcgaccgg cgtactggtc aggcccagat cggcgttgcc
     1201 ctcgagcagc cggtccagca cgccgtcggc gcgcgccgac cagacgcgca gacgtacctc
     1261 cgggtaggca gcgcgatagt cggcaagcag acgaggcagc gtgaataggc ccacggtcgt
     1321 cacgcaggcg atcgatacct cgccccgctt ggggtcagag agcgacgaaa cgtcgcttcg
     1381 caggtgctct acctgcgcga ggatgtggcg agcgtgtgca tacgccgtct ggccgacggt
     1441 agtcaggtgt aagccgcgtc cgccgcgctc gaccagcggg acaccaagct cgcgttccaa
     1501 cgctgcgatc tggcgcgtca ccgccggctg actgacgttg aggagctctc cggcgcgggt
     1561 aaagcttcct tcttcaacga cccgcacgaa cgtttcgagg tgctgcaaca gcatgaacgg
     1621 tccctccgaa gcgacgattc ccgattcgtc gagcgtatgc tcattatagc gactatgcat
     1681 tggacgtatg gcaaatggag aggtacgttg agcacaagat gactggaatg aatcggacag
     1741 gagggggcgt catgctccgc gagatcgtct ggaagatcgg tggcgaacag ggccaggggc
     1801 tcgaccccac cagtgacgtg ttcgcaacgg tttgtaatcg aatgggctac tgggtgtacg
     1861 cgtacaagac gttttcgtcc cgcatcaagg gcgggcacac cgactttacc gtacgcgtcg
     1921 gccaggatcg caagctttcg cccgctccaa agctgcacat gttggtcgca atggaccagg
     1981 agtctatcga catcctggtc gaggacctga tgggagacgg catcctgctc gccgatagcg
     2041 gcttcaagcc acaggctccg gacggtgtca acatgattac cgccgacctg accaagatgg
     2101 cgcgcgacct cggcaacccc gtttaccgca acatgatcgc ggtcggcgcg tcggcgcagg
     2161 tgcttggcat ccccctggag ccattcaaag agtacgtaca ggagaagttc ggtcgcaaag
     2221 gcgagtctgt tgtaaaaggc aactggaccg cgctagacca gggtgcccag atcgcccgcg
     2281 atcaggtggg cgataagggc ttccgtctcg gcgatccgga tgggaagtcc gacaggctgc
     2341 tcgtaacggg caacgacgcg atgacgctcg gtgctctcgc cgccggctgt cgcacgatct
     2401 ttgcgtaccc gatcacgccg gcttcggaag tgctcgaggt cggggccaag atcttcccta
     2461 agttcggcgg cgtttccgtc cagatggaag atgagcttgg cgccctcggc gcgtgcgtcg
     2521 gcgccggatt cgccggggcg cgagcgctga cgtgtacctc cggccctggt atctccctga
     2581 tgcaggagtt gatgggcttg gcagggatga ccgaagtccc ggttgtgatc atcgatacgc
     2641 agcgcggcgg cccttccacc ggtatgccga ccaagcagga gcagagcgac ctttttgcgc
     2701 tgattcacgg tgggcacggc gaagcgccac gtattgtgct ggcgccgggc acgggcgagc
     2761 aggcattcta cgacatcgcg ctcgctttca accttgccga taactaccat tgcccggtca
     2821 tcctggcgtc ggacctgggc ctggcgttgt ggcaacagac cgtcgatccc ttcgacgtta
     2881 cgaaggtcgt catcgaccgc ggtcctatcg tcgagcagtc tgagctcgac aagatcggcc
     2941 gcgacggttt cgagcgctac gccgacagcg aaaccggcgt tagcgcccgc tcgttccc
//
LOCUS       DATCPE010000085         7806 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U7219
            k141_114164, whole genome shotgun sequence.
ACCESSION   DATCPE010000085 DATCPE010000000
VERSION     DATCPE010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955033
            Sequence Read Archive: SRR1586279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 7806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 16:49:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 640
            CDSs (total)                      :: 631
            Genes (coding)                    :: 627
            CDSs (with protein)               :: 627
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7806
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114164"
                     /isolate="SMAG_U7219"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            196..510
                     /locus_tag="VIM45_02560"
     CDS             196..510
                     /locus_tag="VIM45_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8820200.1"
                     /translation="MVRVRLISKEEAQNTRKTKTPGIRRQRMNQFDEYARSVLDNPEE
                     AAVYEELGENPQKFVLSLRGSFKRAGVNATVRKMRGRDEVRVWISDEPPSATSRRGRG
                     TN"
     gene            complement(608..1972)
                     /locus_tag="VIM45_02565"
     CDS             complement(608..1972)
                     /locus_tag="VIM45_02565"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY8820201.1"
                     /translation="MVIRQRPASFAISLALVLIAGLAATWWATGGPFSQAARDVGSAG
                     SAAGSDPAISTDRAVATLQNRLQQEPTFAAGYAQLGDAYLQKARETADPSYYVKADAV
                     LRQALSLDAQSFDAMVGLGSLALSRHQFRDALQWGEQARVLNPYSVDAYAVIGDAHTE
                     LGEYDEAIAAFQKMIDLKPGLPAYSRVSYARELHGDADGALAAMTKAVKLGGPGTEAT
                     AWTRVQVGNLYFNRGDYTNASRYYKQSLFDYKDYSHGYAGLAKVAAARGHYADAIKLY
                     QRVTRLLPLPEYVIALGDVQQAAGKTQDAARTYDLVDVEDQLLRAANVDSDVEIAQFD
                     VDHGRNVATVLQQAQQGAERRPSVKGYDVLAWTLYKTGDVTGAQSAMKQALKLGTKDP
                     LMLFHAGMITYAAGDHSAARGYLESAVTINPHFSVLYETTATRLLDQLRHEAGARTTS
                     GGSK"
     gene            complement(2057..3442)
                     /locus_tag="VIM45_02570"
     CDS             complement(2057..3442)
                     /locus_tag="VIM45_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013284159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4331 domain-containing protein"
                     /protein_id="HEY8820202.1"
                     /translation="MRKITVFAALVTVALPLGARHATHSVNASSHREAPLTSQYPNID
                     STDLYAFVSPDKPDTVTIIANYIPLEGPAGGPNFYRFGDDTLYTINVDNVGDGLPHIR
                     YDFTFTTFYRNPNTFLYNTGPIKSLDDPNWNIYQSMKVTRTDSTGTKVVGDNLKTPPV
                     NVGSHSTPNYDALASAAIQDVGNGTKVFAGQRADPFFVDLGATFDLLTIRNIPGNMGG
                     GVNTLKGFNVHTIAIQVPISQLTNDGKAPNDTKVSDAVLGFWTSASLQRTSVLRDDGS
                     VADDGPFVQVSRLGMPLTNELVVPVGLKDYWNATAPAADSQFGAAAADPELAHLFTAL
                     YNIKVPPAPRKDIIAAFFTGLPGINMPSNPTIADELRLNVAIPPSATPNRMGVLGNDL
                     AGFPNGRRLTDDVVDIELNVVAGVAYPLFVDKTFQPDPLAGKLGDGVDTPDKPQLSTF
                     PYVASPYAGFP"
     gene            complement(3684..4355)
                     /gene="nth"
                     /locus_tag="VIM45_02575"
     CDS             complement(3684..4355)
                     /gene="nth"
                     /locus_tag="VIM45_02575"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEY8820203.1"
                     /translation="MPPSVPELIERLGRVFGEMQWRPSGDPVGELVLTILSQHTSDRL
                     SGRAFANLLAQFPSWEAVLQAPTSEVAECIAIGGLSNQKAPRIKAVLERIHSERGSLD
                     LHFLSGLPLDEARTWLVDLPGVGPKTAACVLMFALGKPALPVDTHVYRVGQRLGLIPP
                     KMTADKAHAHLESIVPPADVYAFHVGLIKHGRYTCVAQRPRCDGCVLSDVCPACSSFK
                     SSGTG"
     gene            complement(4336..5505)
                     /locus_tag="VIM45_02580"
     CDS             complement(4336..5505)
                     /locus_tag="VIM45_02580"
                     /inference="COORDINATES: protein motif:HMM:NF017634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045550 - geranylgeranyl reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0030494 - bacteriochlorophyll biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranyl reductase family protein"
                     /protein_id="HEY8820204.1"
                     /translation="MHFDTDVVVVGAGPAGATAARALAERGVKTLLIERSRLPRYKSC
                     AGGIPVRTAAMLPFDLEPVIEDRVAGLNVSYQGTSRFTRRSNEPFAFMVMRDRFDSLL
                     TEQACQAGAELADCSPVRELHRIEGGFELTAGGRRMRSRFVIGADGANSVVARATGLG
                     AGLAESAALEAEVCATDTDLERWRGLVNVDFGYRPWGYGWVFPKEQRLSVGLVLPRDR
                     GGDLRGYLRTYLDRLGLGGAEVDRLVGHKLLLRRGNEPIAGHGVLVVGDAAGLADEFT
                     EEGIYYAVKSGLIAADVIGQALEQGFAWLGAYEHQVDRHIMPELRAARIIAYTFYAMM
                     RRSPAAMMGISRYMSYFWNAFFRVQQGVTGYDEELARAWWLRPMLPLVNRASLRS"
     gene            5676..6725
                     /gene="selD"
                     /locus_tag="VIM45_02585"
     CDS             5676..6725
                     /gene="selD"
                     /locus_tag="VIM45_02585"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:5724..5726,aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HEY8820205.1"
                     /translation="MASAPVRLTSMVSCGGUASKVGPAALAQVLRPLTETLSAADFPN
                     LLVGLQLADDAAVYRLNDDQAIVQTLDFFAPVLDDPYLFGAVAAANAMSDVYAMGGDV
                     LFALNIAAFPDSMDIAAMTEIFRGGADKVREAGGVIAGGHTVYDPEPKYGLAVTGAVH
                     PDRVLTKAGAHAGDRLYLTKRIGTGVLCSSIKDGKVAESDLKQATASMLQLNRGGSLA
                     AREAGASALTDITGFGLLGHADEMARLSSVCLVIKAAGVPLLDGALDAVRAGVRTGGS
                     NRNEEFVCDRVRVDPGVPREIVELLYDPQTSGGLLAAVPPERASTFEKACTRHGAGCW
                     SIGHVVAGSGLVVTM"
     gene            6776..7531
                     /locus_tag="VIM45_02590"
     CDS             6776..7531
                     /locus_tag="VIM45_02590"
                     /inference="COORDINATES: protein motif:HMM:NF018822.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LppX_LprAFG lipoprotein"
                     /protein_id="HEY8820206.1"
                     /translation="MNTNHQKSRSAFALALALLLLPAAVACGSKKKSAVATATSAPIA
                     RATPSPTPQDLLNTATKNTEVLKAFHFTLTHENGSTPVAQGISMSKADGDFVQPDRMK
                     ATISGTVAQGFDIDAKVISVGNNVWIALIGNRYLPLPNGVGAASILDPNNGVLKALHG
                     VKSPQFAGTEKVSGVDATVVSGTIDAGDLTALDADAQAGKSVKGKVWIGNTDQRVYRL
                     RLDGPLNDQEPANIARILDLSQFNESVTIQPPA"
     gene            7528..>7806
                     /locus_tag="VIM45_02595"
     CDS             7528..>7806
                     /locus_tag="VIM45_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8820207.1"
                     /translation="MSDPVPAAASGTAHPPSAAALALEHGMRTVSRRPTAALLLFVVG
                     AGVFFGTLDQTVVVTVLPDIIKDIRLPINTKFGEASWIVNGYLIGYTVS"
BASE COUNT         1489 a         2316 c         2485 g         1516 t
ORIGIN      
        1 ctttctgttg tcagcatcag tcatcatctg ataatagtgc acagcttgtc agttcggcct
       61 acgaccgcga atggcgagtc cttacgatcg tattgagcac tcaatttgtg aacaatggcc
      121 ttcatggacc attgcatcgg aactatcgtt gctgtacaac tgaaatgaga gcgatgtctg
      181 catgaagggg gaatgatggt tcgcgttcgt ctcatttcca aggaagaagc ccagaacacc
      241 agaaaaacca aaacgccggg tatacgcagg cagcgaatga accagttcga cgagtatgct
      301 cgctcagtgc tagataaccc ggaggaggct gcggtctacg aagaactggg agagaatccg
      361 cagaaattcg tcttatctct gcgtgggtcg tttaagcggg ccggagtgaa cgccactgtt
      421 cgcaagatgc gcggtaggga cgaagtccgg gtgtggatct ccgatgagcc gccttcggcg
      481 acgtcacgcc gcggccgggg taccaattag cggattcccg cagacagttt gtggttcgcg
      541 ccgcgctcgg taattggcgc cgatcgcgcg caagcacgta cggcgcgaag cgagcccttg
      601 cccgccttca cttggagcct ccgctggttg tgcgagctcc ggcctcgtgg cgtaattgat
      661 cgagcaatcg cgtggcggtg gtctcgtaga gcaccgagaa atgcggattg atagtgaccg
      721 cggattccag gtaaccgcgt gcggcggaat ggtcgccggc cgcgtaggta atcatgccgg
      781 cgtggaacag catgagcgga tctttcgtgc ccagtttcag agcttgcttc atcgccgact
      841 gggctccagt cacgtcaccg gtcttgtaga gggtccatgc cagcacgtcg taccccttga
      901 cgcttggccg ccgttcggca ccttgttgcg cctgttgcag aacagtggcc acgttgcggc
      961 cgtggtcgac gtcgaactgt gcgatctcta catcgctatc gacgtttgcc gcccgaagaa
     1021 gctggtcctc gacgtcgaca aggtcgtacg tacgggccgc gtcctgcgtt ttgcccgcgg
     1081 cctgctgaac gtcgccaagt gcgatcacgt attcgggaag cggtaagagg cgcgtaacgc
     1141 gctggtagag cttgatcgca tcagcgtaat ggccgcgtgc cgcagcaacc tttgccaggc
     1201 ccgcatagcc gtgcgagtag tctttatagt cgaacaagga ctgcttgtag taccggctgg
     1261 cgttcgtgta gtcgccacgg ttgaaataaa gattcccgac ctgcaccctc gtccacgcgg
     1321 ttgcctcggt cccggggcca cccagtttca ccgctttggt cattgctgcg agcgcgccat
     1381 cggcgtcgcc gtgcaactcc cgtgcatacg agacgcgcga atatgctgga agcccgggct
     1441 tcaggtcgat catcttttgg aatgccgcta ttgcttcgtc gtattcacca agctccgtgt
     1501 gtgcatcgcc gatcaccgca taggcatcca cgctgtacgg attcagcacc cgtgcttgtt
     1561 cgccccactg gagcgcgtcg cggaactggt gccgagacag cgccagtgag cccagcccaa
     1621 ccatagcgtc gaagctctgt gcgtcgagac tgagcgcctg gcgtaatact gcgtcggcct
     1681 tgacgtagta cgaggggtct gccgtctcgc gggccttttg gaggtaagcg tctcctaatt
     1741 gcgcatatcc cgccgcgaat gtcggttcct gctgcaggcg attctgcaac gtggcgacgg
     1801 cccggtcggt tgagatcgcc ggatccgagc ccgccgcaga gccggctgag ccgacatcac
     1861 gagcagcctg actgaacgga ccgccggttg cccaccatgt cgcagcgagg ccggcaatca
     1921 ggacgagcgc aagcgaaatg gcgaaagagg cgggtcgttg acggatgacc acggtgcagt
     1981 gctccttgtg aggtacggcg cggaaaccgg agttgtagcc gtccgcccgg acgggcggac
     2041 ggctacagca tcgagactag gggaagccgg cgtacggaga ggccacgtac gggaaggttg
     2101 ataactgtgg cttgtctgga gtgtcgacgc cgtctccgag cttgcccgcg agggggtccg
     2161 gctggaaggt cttgtcgacg aacagcgggt acgctacacc ggcaaccaca ttcaactcga
     2221 tgtcgaccac gtcgtcggtg agccggcggc cgttggggaa gccggccagg tcattgccaa
     2281 gcacacccat gcggttcggg gtggccgacg gcggaatggc tacgttgagc cggagctcgt
     2341 ccgcgatcgt cgggttggac ggcatgttga tgcctggtaa cccggtgaag aatgcggcga
     2401 tgatgtcttt acgcggtgcg ggcggaactt tgatgttgta cagggcggtg aagagatgag
     2461 ccagttccgg atccgccgcc gcggcgccga actgtgaatc tgccgccgga gccgtggcgt
     2521 tccagtagtc cttcagaccc acgggaacga cgagctcgtt agtcaacggc atgccgaggc
     2581 gtgaaacctg gacgaacggt ccatcgtcgg ccaccgagcc atcgtctcgc aggaccgatg
     2641 tccgctgcag tgaggccgat gtccagaagc cgagaacggc gtcgctgacc ttcgtatcgt
     2701 ttggagcctt gccgtcgttg gttagctggc tgatcggcac ctggatggcg atggtgtgga
     2761 cgttgaaccc tttgagcgta ttgacgccgc cacccatatt gccggggatg ttgcgaatgg
     2821 tgagcaggtc gaatgtcgcg cccagatcga cgaaaaatgg gtcggcgcgc tgtcctgcaa
     2881 agactttggt accgttgccg acgtcctgga tcgccgctga ggcgagcgcg tcgtagtttg
     2941 gagtcgagtg cgagccgacg ttgacgggcg gcgtcttcag attgtcgcca actaccttcg
     3001 tcccggtcga atccgtccgc gtgaccttca tggattggta gatgttccag ttcggatcgt
     3061 cgagggactt gatcggtccg gtgttgtaca ggaacgtgtt cgggttgcgg tagaaggtcg
     3121 tgaaggtgaa atcgtagcgg atgtgcggca aaccatcgcc gacgttatcg acgttgatcg
     3181 tatagagcgt gtcatcgccg aatcgataga agttcggtcc gccagccggt ccttccaacg
     3241 ggatgtagtt cgcgatgatg gtgactgtgt ccggcttgtc cgggctgaca aatgcgtaga
     3301 ggtcggtgct atcgatattc gggtactggc tggtcagcgg cgcctcacgg tggctgcttg
     3361 cattaacgct atgcgttgca tgcctggccc ccaggggcag cgctactgtg accagagccg
     3421 caaagacggt gatcttgcgc aatgtggtgt tccttccaaa cttgagcatt agctccattg
     3481 atgtggacag actcgcacgg gcattgtcgg cggcgatctt tcatcgcgtg tcacgttgtc
     3541 gctcctttcg tcgtaagcgc ctcgacgtgg gacacgtcga ggccatcaaa cggttactac
     3601 gcgctgcaaa aaaatccgga tcactgtttg caaaagtgat ccggatttcg cgcactcttt
     3661 tggtcccaat gcgagcaata aagctaacca gtgcccgatg atttgaagga agagcacgcc
     3721 ggacacacgt cgctgagaac gcagccgtcg caacggggtc gctgagcaac acaggtgtag
     3781 cgaccgtgct tgatcagtcc cacatggaat gcgtagacgt cggcgggcgg cacgattgat
     3841 tcgagatgag catgcgcctt atcggcggtc attttcggag gaatcaaccc gagccgctgg
     3901 ccgacgcgat acacgtgagt atcgacaggt aatgcgggtt ttccgagcgc gaacatgagc
     3961 acgcaggcag cggtcttcgg tccgacgccc ggcaggtcta cgagccacgt acgcgcttca
     4021 tccagcggaa ggcctgagag gaagtgcagg tcgaggctgc cgcgctcgga atggatccgt
     4081 tctaaaacgg ccttgattcg gggtgccttc tgatttgata ggccgccgat ggctatgcac
     4141 tctgcgacct ctgacgtggg cgcctggagg acggcctccc agcttgggaa ctgtgcgagt
     4201 aggttcgcga aggcccgtcc ggagagccga tccgacgtgt gctgagagag gatcgtaagg
     4261 accagttccc caaccggatc gccgctcggc cgccactgca tctctccaaa gacgcgaccg
     4321 agccgttcga tcagttcagg aacggaggga ggcacggttc accagcggca acatcggacg
     4381 cagccaccag gcccgtgcaa gctcttcgtc gtacccggtc acgccctgct gaacgcgaaa
     4441 gaacgcattc cagaagtagc tcatgtagcg actgatcccc atcattgccg ccggtgaccg
     4501 gcgcatcatc gcgtagaacg tgtaggcaat gattcgggcg gcacgaagct ccggcatgat
     4561 atggcgatct acctggtgct cgtaggcgcc gagccaggca aaaccttgtt ccagcgcctg
     4621 tccgatgaca tctgcggcaa tcagcccgct cttcaccgca taatagatcc cttcctcggt
     4681 aaattcgtct gcgagcccgg cggcgtcacc aaccaccagg acaccatgac cggctatcgg
     4741 ctcattgccc cgacgtaaga gcagcttatg cccgacaagc cgatccacct cggcaccacc
     4801 gagtcccagt ctgtccaaat aggtgcgcag ataaccgcgt agatcgccgc cccggtcgcg
     4861 cggtaaaaca agcccgacag agagccgctg ttctttcgga aaaacccagc cataccccca
     4921 tggacgatag ccgaaatcga cgtttacgag cccgcgccag cgctccaggt cggtatctgt
     4981 ggcgcacact tccgcttcca aagcagcgct ttctgccagc ccggcaccca ggccggtcgc
     5041 acgtgctacg acgctgttgg caccgtcggc gccgatcacg aaccggctgc gcatccgacg
     5101 tccgccggcc gtcaactcaa aaccgccttc aatcctgtgg agttcacgga cggggctgca
     5161 gtcggctagt tcggcgccgg cctgacatgc ctgctccgtg agcaggctgt cgaagcggtc
     5221 gcgcatcacc atgaacgcaa atggttcgtt ggatcgccgt gtgaagcggc tcgtcccctg
     5281 gtagctgaca ttcagacccg cgacgcgatc ctcgatcacg ggctcaaggt cgaacggcag
     5341 catcgccgcg gtccgaacgg ggatgccgcc cgcgcaactc ttgtaacgcg gcagccgcga
     5401 tcgctcgatc agcagcgttt tcacgccgcg ttcggccagg gcacgtgcgg cagtggcgcc
     5461 agcgggaccc gcgcccacaa ccacaacgtc ggtgtcgaaa tgcattcggg ttcctgtccc
     5521 gccgctcgta cgcgtacgcc acggataagt atagagggcc gcgcggagcc ggacgaatga
     5581 gaggtgccgt gaacgatgcg tggcagtaag cttgcggggc agaggcgcgg actcgtacga
     5641 tcaccgcagc ccgcgagggg aggaggtgat gcgcgatggc gagcgccccg gtgcgcctaa
     5701 cgtccatggt cagttgcggc ggttgagcct ctaaagtggg ccccgcggcc ctggcgcagg
     5761 tgctgcgccc gctgaccgag accttgtccg cggctgattt tccgaacctg ctggttggcc
     5821 tccagctagc ggatgatgcc gccgtttacc ggctgaacga tgatcaagcc atcgtgcaga
     5881 cgctggactt ctttgcgccg gtgctggacg acccctatct gtttggagcc gtcgccgcgg
     5941 ccaacgccat gagcgatgtg tacgccatgg ggggcgacgt tctgttcgcg ctgaatatcg
     6001 ccgcctttcc ggactccatg gatattgccg ccatgacgga aatcttccgt ggcggcgccg
     6061 ataaagtgcg cgaggcgggc ggtgtgatcg ccggggggca tacggtgtac gacccggagc
     6121 caaagtatgg gctggcggta acgggcgccg tccacccgga tcgggtgctg accaaggcgg
     6181 gcgcacacgc gggtgaccgg ctctatttga cgaagcggat cggcacgggc gtgctctgct
     6241 cgagcattaa ggacggcaag gtggccgagt ccgacctgaa gcaagcgacc gcgtcgatgc
     6301 tgcagctcaa tcgtggtggc tcgctcgctg cacgcgaggc gggcgcgtct gcgctgactg
     6361 atatcacagg tttcggtctg cttggccatg cggacgaaat ggcgcggctc agcagcgtgt
     6421 gcttggtcat aaaagctgcc ggcgtgcccc tgctcgacgg cgcgctggat gccgtgcgcg
     6481 ctggcgttcg gacggggggg agcaatcgca acgaggagtt cgtatgcgat cgggtgcgcg
     6541 tggaccccgg cgtgccccgt gaaatagtcg agctgctgta cgacccgcaa acttccggcg
     6601 gactgctggc cgcagtgccc cctgagcgcg ccagtacgtt tgaaaaagcc tgcacgcgac
     6661 acggcgccgg ctgctggtcg atcggccatg tggtcgctgg tagcggactc gtggtcacta
     6721 tgtgacggcg tccggcgcct cacgggcgct ggatgcgccg agaacggatt aagcggtgaa
     6781 cacaaaccat cagaaatctc gatcagcgtt cgcgcttgct ctggcactgc tgcttttgcc
     6841 ggccgcggtc gcgtgcggct cgaagaagaa aagcgcggtg gcgaccgcaa cctccgctcc
     6901 gatcgctcgc gcaacaccgt ctccgacccc gcaggacctg ctcaacaccg ccacgaagaa
     6961 caccgaagtt ctgaaagcat tccacttcac attgactcac gaaaacggca gtacgccggt
     7021 tgcgcagggc atttcgatgt ccaaagcaga tggtgatttc gtgcagccgg atcgaatgaa
     7081 agcgaccatc tcgggcactg tcgcccaggg atttgacatc gacgcaaagg tgatcagcgt
     7141 tggcaataac gtctggatcg cgctgatcgg aaaccgctat ttgccgcttc ccaacggggt
     7201 cggcgcggcc tcaatcctcg acccgaataa cggcgtgctc aaggcattgc acggtgtgaa
     7261 gtcgccccag tttgcgggaa cggaaaaagt tagcggcgtg gatgcgaccg tcgtgtccgg
     7321 cacgattgat gccggcgacc tcaccgccct cgatgctgat gcccaggcag gcaagtcggt
     7381 aaaggggaag gtctggatcg gtaacacgga ccagcgggtc taccgtctgc ggctcgacgg
     7441 tccgctgaac gaccaggagc cggccaacat cgcacgtatt ctcgacctct cacagttcaa
     7501 cgagagcgtc actattcagc cgccggcatg agcgacccgg tcccggccgc tgcctccggc
     7561 actgcgcatc ctccgagcgc ggctgcgctg gcgctcgaac acgggatgcg caccgtgtcg
     7621 cgacgaccaa ctgccgcgtt gctgttgttt gtcgtcggcg cgggtgtgtt ctttggaacg
     7681 ctcgatcaaa ccgtcgtcgt cacggttttg cccgacatca tcaaggacat tcgtctcccg
     7741 atcaatacga agttcggcga ggcatcgtgg atcgtcaacg gctacctgat cggctatacg
     7801 gtttcg
//
LOCUS       DATCPE010000086         5597 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U7219
            k141_146588, whole genome shotgun sequence.
ACCESSION   DATCPE010000086 DATCPE010000000
VERSION     DATCPE010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955033
            Sequence Read Archive: SRR1586279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 5597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 16:49:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 640
            CDSs (total)                      :: 631
            Genes (coding)                    :: 627
            CDSs (with protein)               :: 627
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5597
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146588"
                     /isolate="SMAG_U7219"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..613
                     /locus_tag="VIM45_02600"
     CDS             <1..613
                     /locus_tag="VIM45_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEY8820208.1"
                     /translation="RWEEIADNYHQGQMVVGRTTKLVTFGAFARIEGPVEGLIHVSEL
                     VERRITHPKEVVREGDILPLRIVRIERDRHRLGLSLKAARDQAEAEGWEFDNDGRVSY
                     VPADIVASVEARLGGPPPEAAEPEYSDAQEQSEAPPAGEHETPDPHETPATASQEAAD
                     VSAEALPETESPPESEPSEHEPDENGRMPVERPADSTGAAKDR"
     gene            692..3154
                     /locus_tag="VIM45_02605"
     CDS             692..3154
                     /locus_tag="VIM45_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEY8820209.1"
                     /translation="MADRFDKFSERARRVLTLAQEEAQRFNHNYIGTEHLLLGLVREG
                     DGVAAKVLANLGVELNKVRSAVEFIIGRGDRAVLGEIGLTPRAKKVIELAVDEARRLN
                     HGYIGTEHLLLGLVREGEGIAAGVLESLGVNLERVRAETTRILAQNQPQGAQHPSGRA
                     ASRTPTVDQLGMDLTAAARAGKLDPVIGRNKEIERVIQILSRRQKNNPVLIGEPGVGK
                     TAIAEALAHRIVAGEVPETLQGKRLLTLDIGSLVAGTKYRGEFEERLKKVIEEIKASG
                     NCVLFIDELHMLVGAGAAEGAVDAANILKPSLARGELQCVGATTLDEYRKHIERDAAL
                     ERRFQPIVVDEPSVEDTIEILRGIKEKYEEHHNLTITDDALKAAAELAYRYVADRFLP
                     DKAIDLVDEAASRVRIKRSSTPPSLKEAMKGLEGLRREKDAAISSQQYEYAAELRDRE
                     VKLQDKIEKMEEGWESEREDSKPMVTEEDIAEIVSMWTGIPLARIASEESTRLLHMEA
                     SLHGKVIGQDEAITAVARSVRRARAGMKDPRRPIGVFMFLGPTGVGKTETARALAEFM
                     FGSQDSMIKLDMSEFMEKHSVARLVGAPPGYIGYDDGGQLTDTVRRKSYCLILLDEIE
                     KAHPDVFNMLLQIFDEGHLTDAKGRRVDFRNTIIIMTSNVGAQLIRRNSQLGFVREDE
                     AKDADTQYTRMRDKVLNEMKNVFKPEFLNRLDNTVVFHALTRQHIRQVVDIQLRDVEK
                     QLMAKGVHLEVTEKAKDWLGDKGYDPAFGARPLRRVIQNEIEDRLSESLLQGRFAIGD
                     NIVIDAEEDKLVFNGAAEPALA"
     gene            3311..4687
                     /gene="radA"
                     /locus_tag="VIM45_02610"
     CDS             3311..4687
                     /gene="radA"
                     /locus_tag="VIM45_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012983997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEY8820210.1"
                     /translation="MAELKKARAIFVCQECGLESVRWQGRCPECNAWNTFVERTVRPQ
                     APSRAIASRGAAVLPVELAMVDQDGHSRLPLGIAEFDRVLGGGVVPGSLVLIGGDPGI
                     GKSTLLLQVTAEVAATGKKVLYISGEESAHQLKLRASRLGISGERLFLLTETNIDEAL
                     VTADRLQPDIVVVDSIQSVFSPELPNAPGTAVQLRESTQRIMRWAKVGGVPAFIVGHV
                     TKEGEIAGPRLLEHLVDVVLYLEGERFSSYRLLRGVKNRFGATSEVGVFEMRGSGLSG
                     VENPSEIFLAERAEGAVGSAIVPVVEGTRPVLVEVQALTSQSPLAMPRRTSHGLEVNR
                     LLLIAAVLTKRVGLQLYNQDIIVNVAGGLRVQEPATDLGVALAIASSFKDIKLPGDMV
                     AIGEVGLSGELRSVGHLDRRLSEAEKLGFKNALLPVAALRRYTPQTTMTVHAAPSLSD
                     AIERAFSL"
     gene            complement(4697..>5597)
                     /gene="rodA"
                     /locus_tag="VIM45_02615"
     CDS             complement(4697..>5597)
                     /gene="rodA"
                     /locus_tag="VIM45_02615"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HEY8820211.1"
                     /translation="NLAPILYGLTIVGLLAVLVIGQRAFGSRRWFSLAALQLQPSELA
                     KLVTIIFLAKFLSDDRRRIHTVRGFLTAFGIAAVPALLVFVEPDLGTAIVFLAVWLGM
                     IYVAGARARHLLTFVGACVASIPFIVVAGLSGYQKDRFQSFIDPGKDPLGAGFNILQA
                     QISIGSGGLFGKGWTHGPQTQLAYLRTQTTDYIFSVLGEELGFIGALALFFLFIVLLF
                     RGLRTASRSNDDFGRYIATGIVVMIVVQLFINIGVNVRLFPVTGIPLPFISQGGSSLL
                     TMFAAIGILQSIAVRRRATPFRF"
BASE COUNT         1160 a         1645 c         1852 g          940 t
ORIGIN      
        1 gcgatgggaa gagatcgccg acaactacca tcagggtcag atggtcgtcg gacgtaccac
       61 gaaactggta acgttcggcg cattcgcccg gatcgagggt cccgtcgagg ggctgatcca
      121 cgtttccgag ctggtcgagc gacgcattac ccacccgaaa gaggtggtgc gagagggcga
      181 catcctgccg cttcggatag tgcgtattga gcgcgaccgg cataggctgg gattgagcct
      241 caaggcggca cgcgatcagg cagaggcaga gggctgggag ttcgacaacg atgggcgcgt
      301 gagttacgtg cccgcggaca tcgtcgcgag cgttgaggct cggcttggtg gaccgccgcc
      361 cgaggcggcc gaacccgagt atagcgacgc gcaggagcag agcgaggcgc ctccggccgg
      421 cgaacacgaa acgcccgatc cccatgagac gcctgcaaca gccagtcaag aagcggcgga
      481 cgttagtgcg gaggcactgc cagagaccga atcgccgccg gagtccgagc cgagtgagca
      541 tgagcccgac gaaaacgggc gaatgcccgt ggaacgtccg gcagatagca ctggagcggc
      601 aaaggaccga taatagatct gtaacccatc gcggtgcggc cgcgagtcag tatggcgcgc
      661 tcgatccaga cttcgggcca cggagacaag tatggcggac agatttgaca agtttagtga
      721 acgagcacgc cgggtcctga ctctcgctca ggaagaggcg cagcgtttca accataacta
      781 catcggcacg gaacacctgc tgcttggcct cgtgcgggag ggtgacggcg ttgccgcaaa
      841 ggtgctggcc aacctcggcg ttgagctgaa caaagtacgg tcggcagtgg agttcatcat
      901 cggacgcggg gaccgcgccg tattgggcga gataggactg acgccgcgcg ccaagaaggt
      961 gatcgagctt gcggtagacg aagcccgccg cttgaatcac ggttacatcg gtacggagca
     1021 cctgctgctt ggcctggtcc gcgagggcga gggcatcgcg gctggagtgc tggagtcgct
     1081 gggggtgaac ttggagcgcg tgcgcgccga gacgacgcgc atcctggcac agaatcaacc
     1141 gcagggtgcc cagcatccgt ccggacgggc ggctagccgc acgcccacgg tcgatcagct
     1201 cggcatggat ctgaccgcgg cggcacgcgc cggcaagcta gatccagtca tcggccggaa
     1261 caaggaaatc gaacgggtca tccagatcct gtcgcggcgg cagaagaaca accccgttct
     1321 catcggcgag ccgggcgttg gcaagacggc gatcgccgag gcgctagcgc atcgcatcgt
     1381 cgccggcgag gtgccggaga cgctgcaggg caagcggctg ctgacgctcg acatcggatc
     1441 gcttgttgcc ggtacgaagt accgcggcga gtttgaagag cggctcaaga aggtaatcga
     1501 ggagatcaag gcctcgggca actgtgtcct cttcatcgac gagctccaca tgctcgtggg
     1561 cgccggcgcg gccgagggcg cggtggacgc cgccaatatc ctgaagccgt cgctggcacg
     1621 cggcgagctg cagtgtgtcg gcgcgacgac gctcgacgag taccgtaagc acatcgagcg
     1681 cgatgcggcg ctcgagcgcc ggttccagcc gatcgtcgtg gacgagccca gcgtcgagga
     1741 tacgatcgag atcctgcgcg gaatcaaaga gaagtacgaa gagcaccaca atctcacgat
     1801 caccgatgat gcgctcaagg cggccgcaga gcttgcctat cggtacgtcg ccgatcgctt
     1861 tctgcccgat aaggccatcg accttgtgga tgaggccgct tcgcgggttc ggatcaagcg
     1921 gtcatccacg cctccgtcgc tgaaggaagc gatgaagggc ctcgagggcc ttcgccgcga
     1981 gaaggacgcg gcgatctcgt cccagcagta cgagtatgct gcggagctac gcgaccgcga
     2041 ggtcaagctg caggacaaga ttgagaagat ggaagagggc tgggagtccg agcgtgagga
     2101 ctccaagccg atggtgaccg aggaggacat cgcagagatc gtcagcatgt ggaccgggat
     2161 tcccctggcg cgaatcgcca gtgaggagtc gacccgcctg ctgcacatgg aggcgagcct
     2221 gcacggcaag gtcatcggcc aggacgaagc gatcaccgcc gttgcccggt ccgtgcgacg
     2281 cgcccgcgcc ggtatgaagg atccgcgccg cccgatcggc gtgttcatgt tcctcgggcc
     2341 aaccggcgtc ggtaagaccg agacggcacg tgcattggcc gagttcatgt ttggttccca
     2401 ggactctatg atcaagctcg acatgagcga attcatggag aagcatagtg tcgcccgtct
     2461 tgtcggtgcc cctcctggat acatcggcta cgacgacggt ggacagctaa ccgataccgt
     2521 gcggcgcaag tcctactgcc tgattctgct cgatgagatc gagaaggcgc acccggacgt
     2581 gttcaatatg ctgctccaga tcttcgacga ggggcacctg acagacgcca aggggcggcg
     2641 cgtggatttc cgcaacacga tcatcatcat gaccagcaac gtcggcgccc agctgatccg
     2701 gcgcaactcg cagctcggtt tcgtccgcga ggacgaggcc aaggacgccg atacgcagta
     2761 cacgcgtatg agagacaaag tgttgaacga gatgaagaat gtctttaagc cggagttcct
     2821 gaaccggctg gacaacaccg ttgtctttca cgcgctcacc cgccagcata tccggcaggt
     2881 ggtggacatc cagcttcgcg acgtcgaaaa acagctcatg gccaagggcg tgcatcttga
     2941 ggttaccgaa aaagcaaagg actggctcgg agacaaaggc tacgacccgg cttttggcgc
     3001 tcgtccgttg cgccgcgtta tccagaacga gatcgaggac cgcctgtcgg agtcgcttct
     3061 gcaggggcgg tttgccatcg gcgacaatat cgtcatcgac gccgaggaag acaagctcgt
     3121 cttcaacggc gcagccgagc ccgcgctggc gtaatccggc tgctggctcc gatagagcac
     3181 aaagcaaagg ggcgggcatg acccgcccct ttgctttgtg cctgcgatag cattgtgctg
     3241 agcagtcgat tgcaggctgc tagaatggaa caaaggttct aacgaacgct gagatccgtg
     3301 gagttggcgc gtggctgagc tgaagaaggc acgggcgatc ttcgtgtgcc aggaatgtgg
     3361 gctcgagtcg gttcgctggc agggtcgctg cccggagtgc aacgcctgga atacgttcgt
     3421 cgagcgtacc gttcggccgc aggcgccgag tcgcgcgatc gccagccgcg gtgcagcagt
     3481 cctaccggtc gagctcgcta tggtcgacca ggacgggcac agccgcctgc cgcttggtat
     3541 cgccgagttt gaccgcgtgc tgggcggcgg tgtcgtcccc ggctcgctcg tgctgatcgg
     3601 cggcgacccc ggaatcggaa agtcaacgct attgctgcag gtcaccgccg aggtggcggc
     3661 taccggtaag aaagtactct acatctcggg agaagagtcg gcgcaccagt tgaagttacg
     3721 ggcaagccga ctgggaatct cgggcgaacg gctcttcctg ctgaccgaga ccaacatcga
     3781 tgaagcgctg gttacggcag atcggttgca gccggacatc gtcgtcgtcg actccatcca
     3841 atccgtattt tcgcccgagt tgccgaacgc ccccggaacg gccgtccagc tacgtgagtc
     3901 cactcagcgc atcatgcgct gggccaaggt tggcggcgtc ccggccttca tcgtgggcca
     3961 cgtgaccaag gagggggaga tcgcggggcc acggctctta gaacaccttg tagacgtggt
     4021 cctgtacctc gagggcgaac gattcagtag ctatcgtctg ctgcggggcg tcaagaaccg
     4081 gttcggtgcc acgagcgaag tcggggtgtt cgagatgcgc ggcagcgggc tgagcggggt
     4141 ggagaacccg tctgagatct tcctcgccga gcgcgctgag ggcgccgtcg gcagcgcgat
     4201 cgttcccgtt gtggagggta cgcgtccagt gctcgtggaa gtacaggcgc tcacgtccca
     4261 gagtccactc gcgatgcccc gccgtacctc tcacggcctg gaagtgaacc ggcttctgtt
     4321 gattgcagcc gtgctgacca agcgggttgg acttcaactt tacaaccagg acattatcgt
     4381 aaacgtggcc ggcggccttc gtgtccagga gcccgcgaca gatctgggcg tggcgctggc
     4441 gatcgcgtcg agcttcaagg acatcaagct tcccggcgat atggttgcca ttggcgaagt
     4501 cggcctcagt ggtgagctgc gatccgtcgg ccatcttgat cgccggctga gcgaagccga
     4561 gaagctgggc ttcaagaacg ccttgctacc ggttgctgcg ctgcgccgtt acacgccaca
     4621 aacgaccatg accgtacacg cggcaccctc gctcagcgac gcgatcgaac gcgcgttttc
     4681 tctgtaggta cccggctcaa aatctgaacg gcgtcgctct gcggcgaacg gctatgcttt
     4741 gcaagatgcc gatagccgca aacatggtca gcagggagct tcctccctgg ctgataaacg
     4801 gcagcgggat tccggtgacg ggaaacagcc ggacgttgac accgatgttg atgaacagct
     4861 gcacaacgat catgaccacg attccggtcg cgatgtagcg tccgaagtcg tcgttcgatc
     4921 ggctcgccgt gcgcagcccg cggaacagca gcacgatgaa gaggaaaaag agcgccagcg
     4981 caccaatgaa gcccaactct tcgccgagaa cgctgaaaat gtagtccgtc gtctgagtac
     5041 gcaggtaggc gagttgggtt tgcggcccgt gcgtccagcc ttttccaaat aggccaccgg
     5101 agccaatgct gatttgcgcc tgaagaatgt tgaagcccgc accgagcggg tctttgccgg
     5161 ggtcgatgaa gctctggaat cggtccttct ggtagcccga caggccggcg accacgatga
     5221 acgggatcga tgccacgcag gcgccgacga acgttagcag atgccttgcc cgggcaccgg
     5281 caacatagat catcccgagc caaacggcaa ggaaaacgat ggcggtgcca aggtccggct
     5341 cgacgaacac gagcaacgcc ggcaccgccg cgatcccaaa cgcggtcaag aacccgcgga
     5401 ccgtatggat gcgcctccgg tcatcagaga ggaatttggc gaggaatatg atcgtcacca
     5461 actttgccag ttcggaaggc tgaagctgaa gcgcggcaag cgagaaccag cggcgcgatc
     5521 caaaggcccg ctggccgatc acgagcaccg cgagtagccc gacgatcgtc aatccataga
     5581 gaatgggcgc caggttg
//
LOCUS       DATCPE010000087         2137 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U7219
            k141_212744, whole genome shotgun sequence.
ACCESSION   DATCPE010000087 DATCPE010000000
VERSION     DATCPE010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955033
            Sequence Read Archive: SRR1586279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 16:49:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 640
            CDSs (total)                      :: 631
            Genes (coding)                    :: 627
            CDSs (with protein)               :: 627
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212744"
                     /isolate="SMAG_U7219"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1112)
                     /locus_tag="VIM45_02620"
     CDS             complement(<1..1112)
                     /locus_tag="VIM45_02620"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY8820212.1"
                     /translation="MHGESDRPLISVIVPAYNEELWLEQCVRALQSQETSVLYEIIVV
                     DNNSTDSTAEVAERLGVRVVHESRRGLSFARQAGQDAARGEIVAHTDADSAAPPDWIE
                     SIAQAFEEFPDTAVVSGPMCFPSAPLLMQAVAPIQNFFIWLWWLLTGRLGVLNGCNFA
                     LRAAFLAKAGGFATGLPLTGDSLVLSIMRPYGTARRIGAKMRTSGRRFHGQGTLRVLT
                     FYAHEQFAAALGRERLMSAPDIRLPDGWAVQIHRRRRAALALLPVLPVLVLAGSCAYM
                     AINPTSQVYGRIVLHGPEGEKTVALTFDDGPNEPFTSEILDVLDREGVKATFFAVGVN
                     VETYPDAARRIVADGNVIANHSWDHSRIATAVDFRYS"
     gene            complement(1271..>2137)
                     /locus_tag="VIM45_02625"
     CDS             complement(1271..>2137)
                     /locus_tag="VIM45_02625"
                     /inference="COORDINATES: protein motif:HMM:NF013565.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DALR domain-containing protein"
                     /protein_id="HEY8820213.1"
                     /translation="RVEVDELKEFPGDFALWKGAKPGEPAWESPWGAGRPGWHIECSA
                     MSLRYLGPQIDIHGGGEDLVFPHHTNEIAQTEAYTGKQPFVRIWMHNAHLRFAGEKMS
                     KSLGNILSIRDALERWGADGLRLFVLGSHYRSPLTASDEALDAAKRGAERLKAAARIP
                     GAGSGREVETTVCRARFVEALEDDLNTPQALAALFDLAKEINRGRDDGATIERAQSTL
                     LEMAEILGLRLEESSTAGIAAEPFVELLVEIRRGLRSAKQFQLADRVREGLAALGITL
                     EDAPNGTTWKRG"
BASE COUNT          403 a          697 c          641 g          396 t
ORIGIN      
        1 gagtagcgga agtccaccgc cgtagcgata cgggagtggt cccacgagtg gttggcaatc
       61 acgttgccgt cggcgacgat acgtcgcgcg gcgtcgggat atgtttccac gttgacaccg
      121 acggcgaaga aagtcgcctt caccccttcc ctgtccagga catccagaat ctcggaggtg
      181 aacggctcat tcggtccgtc gtcgaacgtc agggcgaccg ttttctcgcc ttccggccca
      241 tgcaagacga tcctcccgta cacctgcgac gtcgggttga tcgccatgta ggcgcaactg
      301 ccggcgagca ccagtaccgg gaggacgggc aacagcgcta gcgcagcgcg gcgtcgccgg
      361 tggatctgca cggcccagcc atcgggcaat cgaatatccg gtgccgacat caacctctcc
      421 ctgcccagcg cggcagcgaa ctgctcgtgg gcatagaacg tgaggacgcg aagggttccc
      481 tggccatgga aacgtctgcc ggaggtccgc atcttcgcgc cgattcggcg tgctgtgcca
      541 tacggtctca tgatcgacag caccaggcta tcgccagtca acggcaggcc ggttgcaaac
      601 ccaccggcct tggcgagaaa tgcggcgcgc aacgcgaagt tgcagccatt caggacgccc
      661 aaccggccgg tcagcagcca ccacagccag atgaagaagt tctgaatcgg cgccacggcc
      721 tgcataagta agggcgcact ggggaaacac atcgggccgg aaaccacggc cgtatccggg
      781 aactcttcga atgcctgcgc gatcgattcg atccaatcgg gaggcgcggc ggaatcggcg
      841 tccgtgtgcg ccacaatctc gccccgagca gcgtcctgcc cggcttggcg ggcgaagctc
      901 agtccgcggc gcgattcgtg aacgaccctt acaccaagac gctcggcgac ctcggccgtg
      961 ctgtcggtag agttattgtc aacaacgata atctcgtaga gaaccgaggt ttcttgactc
     1021 tgaagcgcgc gtacacattg ctcgagccag agctcttcgt tgtaggccgg aacgatgacc
     1081 gagattaacg gccggtcgct ttcgccgtgc atcacctttg tatccggttt tgggaaacca
     1141 atctgcgatg ccctacttgc tatattatag actttaacat cgcgtaaccc agagcgctgc
     1201 atcccaaagg aacagtggaa ccgggcgctt cgtacgttga ccgcgatgtt acgcggacac
     1261 ggctctgtcc tcaccccctc ttccaggtcg taccgttcgg ggcgtcttcg agcgtaatgc
     1321 ccagggctgc aagtccctcg cgaacccggt cggcgagctg gaactgtttt gccgagcgca
     1381 gcccacgccg aatctcgacc agcagctcca caaagggctc ggcggcaatg cccgcggtgg
     1441 agctctcctc gagccgcaac ccgaggatct ctgccatctc gagcaaagta ctctgcgccc
     1501 gttcgatagt cgcgccgtcg tcacgaccgc ggtttatctc tttcgccagg tcgaacaggg
     1561 ccgcaagtgc ctgtggcgtg ttcagatcgt cttcgagcgc ctcgacgaag cgggcacggc
     1621 acaccgttgt ttcgacttcg cggccgctgc ctgcccccgg aatgcgtgcc gccgccttca
     1681 gccgttcggc gccacgcttg gccgcatcca acgcttcgtc ggaggcggta agcgggctgc
     1741 gatagtgcga gcccagcacg aacaggcgca gtccgtcagc gccccagcgt tcaagcgcgt
     1801 cgcggatgct gaggatgttg ccgagcgact tgctcatctt ttcgcccgca aaccgcaggt
     1861 gggcattgtg catccagatc cgcacgaacg gctgcttacc cgtgtacgct tcggtctgtg
     1921 cgatctcgtt tgtatggtga ggaaaaacca ggtcttcgcc gccgccatga atgtcgatct
     1981 gcggacccag gtagcgcaac gacatcgcgg agcattcgat gtgccagccg ggccggccgg
     2041 caccccacgg actctcccag gcgggttcgc ccggcttggc tcccttccaa agggcaaaat
     2101 cgcccgggaa ctccttgagc tcgtcgactt ccacccg
//
LOCUS       DATCPE010000088         2651 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U7219
            k141_115226, whole genome shotgun sequence.
ACCESSION   DATCPE010000088 DATCPE010000000
VERSION     DATCPE010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955033
            Sequence Read Archive: SRR1586279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 16:49:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 640
            CDSs (total)                      :: 631
            Genes (coding)                    :: 627
            CDSs (with protein)               :: 627
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2651
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115226"
                     /isolate="SMAG_U7219"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..719)
                     /locus_tag="VIM45_02630"
     CDS             complement(<1..719)
                     /locus_tag="VIM45_02630"
                     /inference="COORDINATES: protein motif:HMM:NF024872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY8820214.1"
                     /translation="MAELKKARAIFVCQECGLESVRWQGRCPECNAWNTFVERTVRPR
                     APSRAIASRGAAVLPVELASVDQDGHSRLPLGIAEFDRVLGGGVVPGSLVLIGGDPGI
                     GKSTLLLQVTAEVAATGKKVLYISGEESAHQLKLRASRLGISGERLFLLTETNIDEAL
                     VTAERLQPDLVVVDSIQSVFSPELPNAPGTAVQLRESTQRIMRWAKVCGVPTFIVGHV
                     TKEGEIAGPRLLEHLVDVVLYL"
     gene            complement(876..>2651)
                     /locus_tag="VIM45_02635"
     CDS             complement(876..>2651)
                     /locus_tag="VIM45_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010935865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEY8820215.1"
                     /translation="VAGEVPETLQGKRLLTLDIGSLVAGTKYRGEFEERLKKVIEEIK
                     ASGNCVLFIDELHMLVGAGAAEGAVDAANILKPSLARGELQCVGATTLDEYRKHIERD
                     AALERRFQPIVVDEPSVEDTIEILRGIKEKYEEHHKLTITDDALKAAAELAARYVADR
                     FLPDKAIDLVDEAASRVRIKRSSTPPSLKEAMKGLESLRREKDAAISSQQYEYAAELR
                     DREVKLQDKIEKMEEGWESEREDSKPMVTEEDIAEVVSMWTGIPLARIASEESARLLH
                     MEASLHGKVIGQDEAITAVARSVRRARAGMKDPRRPIGVFMFLGPTGVGKTETARALA
                     EFMFGSQDSMIKLDMSEFMEKHSVARLVGAPPGYIGYDDGGQLTDTVRRKSYCLILLD
                     EIEKAHPDVFNMLLQIFDEGHLTDAKGRRVDFRNTIIIMTSNVGAELIRRNSQLGFVR
                     EDEAKDANAQYTRMKDKVLNEMKNVFKPEFLNRLDSTVVFHALTRQHIREVVDILLRD
                     VEKQLMAKGVHLEVTEKAKDWLGDKGYDPTFGARPLRRVIQNEIEDRLSESLLQGRFA
                     VGDNIVIDTEEDKLVFNGAAEPALA"
BASE COUNT          458 a          880 c          778 g          535 t
ORIGIN      
        1 aggtacagga ccacgtctac aaggtgttct aagagccgtg gccccgcgat ctccccctcc
       61 ttggtcacat gacccacgat gaatgtcggg acgccgcaaa ccttggccca gcgcatgatg
      121 cgctgggtcg actcacgtag ctggacggcc gttccggggg cgttcggcaa ctctggcgaa
      181 aatacggatt ggatggagtc gacgacgacg aggtccggct gcaaccgctc tgccgtaacc
      241 agcgcttcat cgatgtttgt ctcggtcagc aggaagagcc gttcgcccga aattcccagc
      301 cggcttgccc gtaacttcaa ctggtgcgcc gactcttctc ccgagatgta gagtactttc
      361 ttaccggtag ccgccacctc ggcggtgacc tgcagcaata gcgttgactt tccgattccg
      421 ggatcgccgc cgatcagcac gagcgagccg gggacgacac cgccgcccag cacgcggtca
      481 aactcggcga taccaagcgg caggcggctg tgcccgtcct ggtcgaccga agcgagctca
      541 accggtagga cagctgcacc gcggctggcg atcgcgcgac tcggcgcccg cggccgaacg
      601 gtacgctcga cgaacgtatt ccaggcgttg cactccgggc agcgaccctg ccagcgaacc
      661 gactcaagcc cacattcctg gcaaacgaag atcgcccgtg ccttcttcag ctcagccacg
      721 cgccaactcc atcgatctca gcgttcgtta gaacctttgt tccattctag caacctgcaa
      781 tcgactgctc agcacaatgg tatcgcaggc acaaagcaaa ggggcgggtc atgcccgccc
      841 ctttgctttg tgctctatcg gagccagcag ccgggttacg ccagcgcggg ctcggctgcg
      901 ccgttgaaga cgagcttgtc ttcctcggtg tcgatgacga tattgtcgcc gaccgcaaac
      961 cgcccctgca gcagcgactc cgacagacgg tcctcgatct cgttctggat gacgcggcgc
     1021 aacggacgag cgccaaatgt cggatcgtaa cccttatctc cgagccagtc ctttgccttt
     1081 tccgtgacct cgaggtgaac gcccttggcc atgagctgct tctcgacgtc gcggagcagg
     1141 atgtccacca cttcccggat gtgctggcgg gtgagcgcgt ggaagacaac agtactgtcc
     1201 agccggttca ggaactccgg cttaaagacg ttcttcattt cgttcagcac tttgtctttc
     1261 atgcgcgtgt actgcgcgtt ggcgtccttg gcctcatcct cgcggacgaa accgagttgc
     1321 gagttgcgcc ggatcagctc ggcaccgacg ttgctggtca tgatgatgat cgtgttgcgg
     1381 aaatccaccc gccgcccctt ggcgtccgtc aggtgcccct cgtcgaagat ctgcagcagc
     1441 atgttgaaca catcagggtg cgccttctcg atctcgtcga gcaggatcag gcagtatgat
     1501 tttcgccgca cggtatcggt gagctgtccg ccgtcgtcgt agccgatgta tccgggaggg
     1561 gcgccgacca gtcgagcgac gctatgcttc tccatgaatt cgctcatgtc gagcttgatc
     1621 atggagtcct gggaaccaaa catgaactcg gccagcgcgc gtgcagtctc ggtcttaccg
     1681 acgccggttg gcccgaggaa catgaacacg ccgatcgggc ggcgcggatc cttcataccg
     1741 gcgcgggcac gtcgcacgga cctggcaacg gcggtgatcg cctcgtcctg gccgatgacc
     1801 ttgccgtgca ggctcgcctc catgtgcagc aggcgggccg actcctcact ggcgattcgc
     1861 gccagcggaa tcccggtcca catgctgacg acctctgcga tgtcctcctc ggtcaccatc
     1921 ggcttggagt cctcacgctc cgactcccag ccctcttcca tcttctcgat cttgtcctgc
     1981 agcttgacct cgcggtcccg caactcggca gcatactcat actgctgaga cgagatcgcg
     2041 gcgtccttct cgcggcgaag gctctcgagc cccttcatcg cttccttgag cgacgggggc
     2101 gtggatgacc gcttgatccg cacccgcgaa gcggcttcat ccaccaggtc gatggccttg
     2161 tcgggcagga agcgatcggc gacgtacctg gcagcaagct ctgcagccgc cttcagggca
     2221 tcgtcggtga tcgtgagctt gtgatgctct tcgtactttt ctttgattcc gcgcaagatc
     2281 tcgatcgtgt cctcgacgct gggctcgtcc acgacgatcg gctggaaccg gcgttcaagt
     2341 gcggcgtcgc gctcgatgtg cttgcggtac tcgtcgagcg tcgtcgcgcc gacgcactgc
     2401 agctcgccgc gggccagcga cggcttcagg atattggcgg cgtccaccgc gccctcggcc
     2461 gcgccggcgc ccacgagcat gtggagctcg tcgatgaaga ggacacagtt gcccgaggcc
     2521 ttgatctcct cgatcacctt cttgagccgc tcttcaaact cgccgcggta cttcgtaccg
     2581 gcaacaagcg atccgatgtc gagcgtcagc agccgcttgc cctgcagcgt ctccggcacc
     2641 tcgccggcga c
//
LOCUS       DATCPE010000089         3982 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U7219
            k141_149034, whole genome shotgun sequence.
ACCESSION   DATCPE010000089 DATCPE010000000
VERSION     DATCPE010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955033
            Sequence Read Archive: SRR1586279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 16:49:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 640
            CDSs (total)                      :: 631
            Genes (coding)                    :: 627
            CDSs (with protein)               :: 627
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3982
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149034"
                     /isolate="SMAG_U7219"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /locus_tag="VIM45_02640"
     CDS             complement(<1..410)
                     /locus_tag="VIM45_02640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY8820216.1"
                     /translation="MNEESDGSPDPAAELELGIKQVAVQQTPDGSYQIILRTTRGDIP
                     GILHACEGGTGAAVFISGASGGVNGPADSVYERLAFKLSDSGVSSLRLDYRMPGDFDE
                     CVLDALGGVSFLRGIGAERVVVVGHSFGGAVAIKA"
     gene            complement(407..1618)
                     /locus_tag="VIM45_02645"
     CDS             complement(407..1618)
                     /locus_tag="VIM45_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY8820217.1"
                     /translation="MTSEFIRRPLISKRVDAIPASGIRRFFDIIATMDDVISLGVGEP
                     DFVTPEQICEAAIRSIKQGHTHYTSNFGMIQLREAIARDLERRYGVSYDPATELMCVA
                     GVSEALGVTFQAILDPGDEVLSPDPFYVAYPPNVIMAGGDFIAVPTYERNEFRLMVED
                     LEQFVTPKTRALLMGFPANPTGAVMSRSDLEEVAEFVHRHDLLVVIDEIYARLTYRGR
                     HVCFASLPGMKERTILLGGFSKTYAMTGWRLGYVAAPPEALEAMMKVHQYVMMSAPTV
                     AQYAALEALRSGEEDVQLMLAEYDRRRRLVVDRFNAMGLHCFEPRGAFYAFPDISSTG
                     LDDESFAERLLFEERVAVVPGSAFGACGRGHVRACYATSYEKLVEALDRTERFVARCR
                     DENAGVRGGQR"
     gene            complement(1780..3237)
                     /gene="gatA"
                     /locus_tag="VIM45_02650"
     CDS             complement(1780..3237)
                     /gene="gatA"
                     /locus_tag="VIM45_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEY8820218.1"
                     /translation="MEPYYSTIHDAAERLQRRELSARELTQSVLDRIQQVDGRIGAYL
                     TVTAEQALAAADAADARLRAGVSGPLTGIPVCIKDVLCTCDVRTTAGSRMLERFVPPY
                     DATVVAKLKAAGSVMVGKANMDEFAMGSSNENSAFFPVHNPWNLDCVPGGSSGGSAAS
                     VAAGEALYALGSDTGGSIRQPASLCGVVGVKPTYGRVSRFGLIAFASSLDQVGPFTRD
                     VRDAAIVMNAISGHDALDSNSAPVDVPDFTSALTGDVSGLRIGVPKEYFVEGMDSGVR
                     DAVTAAIRMFEQLGATVDWDVTLPSTPHALAVYYIIAPSEASANLARYDGVKYGFSDQ
                     RGASMWENMERTRQFGFGDEVKRRVMLGTYALSSGYYDAYYLKAQKVRTLIRREFDAA
                     FEKYDVLLTPTSPTVAFRIGERSQDPLQMYLSDVCTLPVNIAGLPGVSVPCGFVGGLP
                     VGLQILAKPFDEATMLRAADAYERSTEWAGRHPDL"
     gene            complement(3262..3549)
                     /gene="gatC"
                     /locus_tag="VIM45_02655"
     CDS             complement(3262..3549)
                     /gene="gatC"
                     /locus_tag="VIM45_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011229790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEY8820219.1"
                     /translation="MSLTKQEVEHIARLARVGVTDEDVERFQRQLSVILDSFEQLREL
                     DTEGVEPTAHTLPLRNVERQDLPRLSFPREAMLANAPQVQDGFVRVRKVLE"
     gene            3681..>3982
                     /locus_tag="VIM45_02660"
     CDS             3681..>3982
                     /locus_tag="VIM45_02660"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY8820220.1"
                     /translation="MSAHSPAIAPRFEDERAALGNPSFVWRFGQDRRLDLIRAWVRLD
                     GKRILDVGCGVGMYVRKFRHFSDEVYGIDVAFDRVREGAKELPNLMLAAGEHLPFP"
BASE COUNT          775 a         1247 c         1238 g          722 t
ORIGIN      
        1 gctttgatcg cgaccgcacc gccaaagctg tgtcccacga ccacgacgcg ctccgcgccg
       61 attccacgca gaaaactcac cccgccgagc gcatcaagca cgcactcgtc gaaatcgccg
      121 ggcatgcggt agtccaatcg caggctgcta actccggaat cgctgagttt gaaggccagt
      181 cgctcgtaga ccgagtccgc cggaccgttt acgcctccgc tggcgccaga gatgaatacc
      241 gctgcgccgg tacccccttc acaggcgtgg aggatgccgg gaatatcacc ccgtgtcgta
      301 cgaaggatga tctggtatga accatccgga gtttgctgaa cggcgacctg cttgatgccc
      361 agttcgagct cggcggcggg gtccggggac ccgtcgcttt cctcgttcaa cgttggccgc
      421 ctcggacgcc cgcgttctcg tcccggcacc gcgcgacgaa gcgctccgtt cgatcgagcg
      481 cttcgacgag cttctcgtaa gaagtcgcgt agcaggcgcg cacgtgtccc cgtccgcagg
      541 cgccgaacgc ggagccggga acgacggcga cgcgctcctc gaaaaggagg cgctcagcaa
      601 acgactcgtc gtcaagaccg gtagacgaga tatcggggaa tgcgtagaag gcgccgcggg
      661 gctcgaagca gtgcagcccc atggcattga aacggtccac caccagccgg cgacgacggt
      721 cgtactccgc cagcatcaac tggacatcct cttcgccgct tcggagcgcc tccagcgccg
      781 cgtattgcgc gactgttggc gcagacatca tcacgtactg gtggaccttc atcatggctt
      841 ccagcgcttc cggcggtgcc gccacgtatc cgagccgcca gccggtcatc gcgtacgtct
      901 tcgagaagcc gccgagcaga atggtccgtt ctttcattcc aggcagcgag gcgaaacaaa
      961 cgtgcctgcc acggtaggtc aaccgcgcat agatctcgtc gatcacaacc agcagatcgt
     1021 ggcggtggac gaattcagcc acctcttcaa ggtccgacct ggacatcacc gccccggtcg
     1081 ggttggccgg aaagcccatc agcagcgccc gcgttttggg cgttacaaac tgctccaggt
     1141 cttcgaccat cagccggaat tcgttgcgct cgtaggtcgg aacggcgatg aaatcaccgc
     1201 ctgccatgat cacgttcggc gggtaggcaa cgtagaacgg atcaggagac aagacctcgt
     1261 cgccgggatc gaggatcgcc tgaaaggtaa ctccaagggc ctcgctgacg ccggccacgc
     1321 acatcagctc ggtcgccggg tcatacgaga cgccgtatcg gcgctcgaga tcgcgcgcga
     1381 tcgcctctcg aagctggatc atgccaaagt tggacgtgta atgggtgtgg ccctgcttga
     1441 tcgatcgaat cgcggcctcg cagatctgct ccggagtgac gaaatccggc tccccaactc
     1501 caaggctgat gacgtcgtcc atcgtggcga tgatatcgaa gaagcgtcgg attcccgacg
     1561 ccgggatcgc gtcgacccgc ttcgagatga gcggacgccg gataaattcg ctggtcatca
     1621 tgcccgtggg ctccccgggt ggacggaggc cgctgcgcag cggtcacccc gccggatagc
     1681 agaaatatag cactttgtaa ggagtgtact ggtagagagc cacaggggcc agatggaaag
     1741 gtcgcggatt cagcgggatc gggcgtacaa cgtggatgct cacaggtccg gatgccggcc
     1801 ggcccactcc gtcgatcgct cgtatgcatc ggcagcccgc agcatcgtcg cctcgtcaaa
     1861 cggcttagcg aggatctgca accccaccgg gaggccgccg acgaatccac agggcaccga
     1921 aacacccgga agaccagcga tgttgaccgg cagcgtgcat acatcgctca ggtacatctg
     1981 cagcgggtct tgcgaccgct cgccgatgcg aaaggcgacc gttggcgaag taggggtcag
     2041 caacacgtcg tacttctcaa aggccgcgtc gaattcgcgc cggatgaggg tgcgcacttt
     2101 ctgtgctttc aagtagtacg catcgtagta accggacgag agcgcgtagg tgccgagcat
     2161 gacccgccgc ttcacctcgt cgccgaatcc gaactgacgg gtgcgctcca tgttttccca
     2221 cattgaggcg ccgcgctggt cggagaagcc gtacttgacg ccgtcatagc gcgccaggtt
     2281 cgccgatgct tcgctgggcg cgatgatgta atagaccgca agcgcgtgcg gagtactggg
     2341 gagagtaaca tcccagtcga ccgtggcgcc caactgttcg aacatgcgga tcgccgccgt
     2401 gaccgcgtcc cgcaccccgc tatccatccc ttcgacgaag tactctttcg gcacgccgat
     2461 gcggaggccg gagacgtcgc cggtcagggc cgacgtgaag tcgggtacgt cgacgggcgc
     2521 cgagtttgaa tccagtgcgt cgtgaccgct gatcgcgttc atcacgattg cggcgtcgcg
     2581 gacgtctcgc gtaaaggggc caacctggtc cagagatgaa gcaaaggcga ttaagccgaa
     2641 gcggctcaca cggccgtagg tcggtttgac gccaacgacg ccgcacagcg acgccggctg
     2701 gcggatgctg ccgccggtat cggagcccaa cgcataaagc gcttcgcccg cggcgacgga
     2761 ggccgctgaa ccgcctgacg aaccacccgg cacgcagtcc aggttccacg ggttgtgaac
     2821 cggaaagaac gccgagtttt cattcgacga gcccatcgcg aactcgtcca tgttggcttt
     2881 tccaaccatc acactgccgg cggctttgag ctttgcgacg acggttgcgt cgtatggcgg
     2941 cacgaaccgt tcgagcattc gcgatccggc ggtggtgcgc acgtcgcacg tgcacagcac
     3001 atctttgatg cagaccggaa taccggtgag gggaccggac actcccgcgc ggagacgtgc
     3061 gtcggcggcg tcagcagcgg caagggcctg ctcggccgtg actgtcaggt aggcgccaat
     3121 cctcccgtcc acctgctgga ttcgatcgag caccgattgc gtcagttcac gtgcagaaag
     3181 ttcacggcgc tgcagccgct cggcggcgtc gtggatggtg gagtagtacg gttccagggc
     3241 gggatctcct gtgtccggct gttattcgag gacttttcgc acgcgcacga agccgtcctg
     3301 gacctgtggc gcgtttgcga gcatcgcctc acgcggaaac gagaggcgag gcagatcttg
     3361 ccgttccacg ttgcgcagcg gcagagtgtg cgccgtcggc tcgacgcctt cggtatcaag
     3421 ctcgcgcagt tgctcgaacg agtccaggat cactgaaagc tgccgctgaa atcgctcaac
     3481 gtcctcgtcg gtcactccga cgcgggcaag tcgcgcgata tgttcaactt cttgtttcgt
     3541 caatgacatg gcggagctaa atctccgagc aatatccgcg cttggatcga attgggagca
     3601 cgggggcgaa aataagtata acagcccgtt aacgttccgc gattgaatgg ctcggtagcc
     3661 tttgttacga tcttgcatgc atgtcagcac attccccagc catcgcacca cgcttcgaag
     3721 acgagcgcgc cgccctcggc aacccgagct tcgtctggcg cttcggccag gaccgacgcc
     3781 tcgatctcat tcgcgcctgg gtaaggctcg atggaaagcg gatcctggac gttgggtgcg
     3841 gagtagggat gtatgtccgc aagttccgcc acttcagcga cgaggtgtac ggaatcgacg
     3901 ttgccttcga tcgagttcgc gaaggggcga aggagcttcc taatttgatg cttgccgcgg
     3961 gcgagcacct cccttttccg aa
//
LOCUS       DATCPE010000090         8901 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U7219
            k141_538394, whole genome shotgun sequence.
ACCESSION   DATCPE010000090 DATCPE010000000
VERSION     DATCPE010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955033
            Sequence Read Archive: SRR1586279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 8901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 16:49:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 640
            CDSs (total)                      :: 631
            Genes (coding)                    :: 627
            CDSs (with protein)               :: 627
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8901
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_538394"
                     /isolate="SMAG_U7219"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            30..941
                     /gene="xerD"
                     /locus_tag="VIM45_02665"
     CDS             30..941
                     /gene="xerD"
                     /locus_tag="VIM45_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEY8820221.1"
                     /translation="MDEAVSQFLEFLTVEKGAAQNTIAAYRNDMQQFAAFCSSTRTVG
                     KSSSWNQVDRTRIQEYLRSLQAKEYADATVARKVAAIKSFFGFMQAEGLTSSDPTESL
                     SSPRVGKSLPKPISPQEVDELLEQPLRRSTPEAKRDKAMLELLYATGMRVTELVSLNI
                     TDIILNDGHAYVRCLGKGAKERTVPIHEHAAESLLLYLRDSRRNLVRTTHEKALFVNR
                     RGERLTRQGFWLILKGYAQSAQIKSGVTPHTLRHSFATHMLRGGAPLRHVQELLGHAN
                     ISTTQVYTQLTNEHVREVYDRAHPRAG"
     gene            1112..2446
                     /gene="eno"
                     /locus_tag="VIM45_02670"
     CDS             1112..2446
                     /gene="eno"
                     /locus_tag="VIM45_02670"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013150542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEY8820222.1"
                     /translation="MYVTIMPSGGDVKHATIYGVLAREILDSRGNPTVEVEVTLSNGV
                     TGRAGVPSGASTGAHEAAELRDGDKNRYGGKGVLTAVANVNVEIADAIHDLSVFDQSR
                     IDRAMIELDGTPNKSRLGANAILGVSLAVAHAAAATAGLPLYRYLGGPSANTLPVPML
                     NILNGGKHAENSTDFQEFMVMPTGAESFTRALQVASEIYQSLKSVLHRRGLNTNVGDE
                     GGFAPSLTDNAGAIDLILEAIESAGYRAGEDVHVALDPAASELYVDGKYKLTREDRGL
                     ESGALVALWTDWVRRYPIVSIEDGMGEDDWEGWRLLTERIGDRVQVVGDDLYVTNTER
                     LARGIKERSSNAILIKLNQIGTLTETLATIEMAKHAGFAAVVSHRSGETEDTTIADLV
                     VATNAGQIKTGAPARGERVAKYNRLLKIEQELGPAAKYPGMGAFYNIDRSIR"
     gene            2449..3327
                     /locus_tag="VIM45_02675"
     CDS             2449..3327
                     /locus_tag="VIM45_02675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8820223.1"
                     /translation="MDAVRQPFQRVSPVIFPEHWDLPGKPAQGPAASLDSYRQTDFQL
                     KHDLHLLHEGMNLQLRVVADSYPAKFRTHAAAAFQLYWSRVFHYLSDSALLVSRGSYV
                     GVPPLMRTACECVAAAVQLGGDELPALLDFLETSLRPDEAHRATEVGRGSYHAGGTLA
                     SDARLGLVYRTSTELARPHFGATLIEVAPESNIQKLAVTFGDQAFHFGWAQLELGWLL
                     SLCALILDFAVAADVLGTTDESRAAIEALGERVDAALKQPDRCRVEEVDAGMDRRFLI
                     VNFRRQSAGAPRKLLL"
     gene            complement(3334..3915)
                     /locus_tag="VIM45_02680"
     CDS             complement(3334..3915)
                     /locus_tag="VIM45_02680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine dioxygenase family protein"
                     /protein_id="HEY8820224.1"
                     /translation="MVLTRYSLEDFIQDMTSLIADSPDQPLLFEKGTRLLERLVQDPE
                     AIPLPYRRPSGKGRRPNHGSYALHRGPGLFVSAVVWGPGDHAGPHDHHTWGMIGVVGN
                     GIEETRFRRVDDRDREGFARLVKQRTTRVLPGEVSLLVPEIDEIHQMDNLSDRSTVEI
                     HVYGKDLVGLDRCSYDLETGAIKPLVSGKFDNC"
     gene            4159..5175
                     /gene="gap"
                     /locus_tag="VIM45_02685"
     CDS             4159..5175
                     /gene="gap"
                     /locus_tag="VIM45_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004624585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEY8820225.1"
                     /translation="MATKIGINGFGRIGRQVLKAIIERHPDELEVVALNDLVDTSINA
                     HLLKHDSNYGNFPGTVETREDALIVNGHTIRVTKYPDPGQIPWKAMGVQIVIESTGRF
                     TDAGKARAHIDGGGAKKVIISAPAKNEDVTIVLGVNQDKYDPRKDNIISNASCTTNGL
                     APMVKVLLDNFGIVKGQMTTVHSYTNSQKILDLASDDLREARAGGMNIVPTSTGAARA
                     LRLVIPEVDGKIDGLAYRVPTPTVSIVEIVALTEKDTTKEEINAAICEAAEGKMKGII
                     GLTMEPLVSMDLKGDARSSIVDGLSTNVVAGNLVKVASWYDNEWGYSCRLADLCAFVA
                     KKGL"
     gene            complement(5205..7250)
                     /locus_tag="VIM45_02690"
     CDS             complement(5205..7250)
                     /locus_tag="VIM45_02690"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8820226.1"
                     /translation="MTADRPPLRLITTKDPQHASPLDSSLEAALRLIGCDRGAIVLRK
                     DSDAQVTLTSFNLSPEEEAACRSLARRLWDRHARIGPLSSSLGGSTLLAQPIETGEGQ
                     AALVVAVRGGAGKVDAERLAALRDVASLLSGTVGGSIDSLRAAERITEERRRTDRVLA
                     LNHALQTLAATEDVADACCNLLAELEPQFPGVDLTCVWLLEASGDRLRMVAARGGDED
                     ALETVRVLPLRAGYGVDEAIATSEIQALDFARGGGRSASARLAKRLGTVSVLHVPLRS
                     RNSVTGVLMLGARVKREFSPDERTFLETLGAQLGGQLDAIRQLERAEAERKRLQTLIE
                     TLPAGVAMYDAGGRVIFYNRAVEEIWGYRPVDTEIGRYAEMVGLFTPEGRLLTPMETP
                     LARAVRNGSAEQVQELIVRQSGTGREIPVLVNAAAIRDAEGRLSGVITVYQDITQVRE
                     VDRLKDDFINTVSHELRTPTTTVRGGALTLLKRGDQLEPDVRRQLLQDMADEAERLYN
                     LVEDLLSLARAQAGMQLQPEPMIVHRFVNKVILDLGGRVGNHALTVDVPPDLPLVEAD
                     PTFLEQVLRNLLENAVKFSPKGKRIEISAEARGAEVLFSVLDRGSGIPSADMDRVFEP
                     FYRTADAVRTASQGAGLGLAVSRRLMKIMGGRIWAEARRGGGTAFRFTLPALKDEVE"
     gene            7403..8749
                     /gene="ffh"
                     /locus_tag="VIM45_02695"
     CDS             7403..8749
                     /gene="ffh"
                     /locus_tag="VIM45_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005813193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEY8820227.1"
                     /translation="MFESLTDKLQGVFGRLNSHGTVTEKDLEEAMREVRLALLEADVN
                     YKVVREFIAQVKERALGSQILTSLTPTQQIIGIVNEELVKLLGSEPGHINRAPQPPTV
                     LMLVGLQGSGKTTHCAKLALHLRQRGDKPLLVAADVYRPAAVDQLQALGRQLNVPVYA
                     EGTSVSPATICEHALVEAKRTGATVVIVDTAGRLHVDEVMMAEVVQLRDLLKPQEVFF
                     VADAMAGQDAVKAAEEFHKAVGTTGLILTKLDGDARGGAVLSIRAVTGVGVKFIGIGE
                     KPDALDTFYPDRMAQRILGMGDIQTLVEKAQAAMGDDDVKELERKMRTAQFDLQDFLT
                     QMGKVKKMGRVADLVKMIPGVNKISSQLGVDDMDDGFFKKTEAIILSMTAAERKNPGI
                     LRKGHSAANRKRRIATGSASSVTDVNQLLNQFEDAKKMMKTLASTKGRGLLGSMFR"
     gene            8803..>8901
                     /gene="rpsP"
                     /locus_tag="VIM45_02700"
     CDS             8803..>8901
                     /gene="rpsP"
                     /locus_tag="VIM45_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008398347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEY8820228.1"
                     /translation="MLRIRLRRVGKKKAPAYRIVVADSRAPRDGAFV"
BASE COUNT         1759 a         2841 c         2739 g         1562 t
ORIGIN      
        1 taagtgcagc gggctaggag agccgagcga tggatgaagc cgtcagccag ttccttgagt
       61 tcctgaccgt ggaaaagggc gcggcccaga acacgatcgc ggcataccgc aacgacatgc
      121 agcagttcgc agccttctgc agtagcaccc gtaccgtcgg taagagctct tcctggaacc
      181 aggtcgaccg gacccgcatc caggagtacc tgcgatcgtt gcaagccaaa gagtacgccg
      241 acgccaccgt ggcgcgaaaa gttgccgcga tcaaatcgtt cttcggcttc atgcaggctg
      301 aaggcctcac gagctcagac ccgacggaga gtctctcgtc gccgcgcgtc ggcaaatcgc
      361 tgccgaagcc gatttcgccg caagaggttg acgaactgtt ggaacagccg ctgcgtcgct
      421 ccacgccgga agccaagcgc gacaaggcga tgctcgagtt gctgtatgcg accggcatgc
      481 gcgtcaccga gcttgtgtcc ctgaacatca ccgatatcat tctcaacgac ggtcatgcct
      541 acgttcgctg cctcggcaaa ggagcaaaag agcgtaccgt gccgatccac gagcacgccg
      601 ccgaatcgct cctgctctac ctgcgcgact cccggcgcaa cctcgttcgc acaacccatg
      661 agaaggcgct gttcgtcaac cgtcgcggcg agcggcttac acgccagggc ttctggctga
      721 tcctcaaggg ctacgcccag tcggcgcaga tcaagtccgg cgtcaccccg cacacgctgc
      781 gtcacagctt cgccactcac atgctgcgcg gcggcgcccc cctgcgccac gtgcaggagc
      841 ttctcggcca cgccaacatc tccacgacac aggtctacac gcagctcacc aacgaacatg
      901 tccgcgaggt ctacgaccgt gcccacccgc gggccggatg agacggtacc catcactctg
      961 cccgcgttcc gccgaaaggc ggtccgtccg caaaatgccg cgatccacac ggaccgcggc
     1021 atattgcggt tcagcgttga cccaccgtgt gataccttcc ggtcacggtt gacggctatc
     1081 cgcgggctca ccggcgatac cgatcaccgc tatgtatgtc acgatcatgc cgtcaggggg
     1141 cgatgtgaag cacgcgacga tctacggtgt tcttgcgcga gagatcctcg attcgcgcgg
     1201 taatccaacc gtcgaggtcg aagtcacact gtcaaacggc gtgacaggca gggccggcgt
     1261 cccctcgggt gcctccacag gggcccatga agccgccgag ctgcgggacg gagacaagaa
     1321 ccggtacggc ggcaaaggtg tgctcacggc tgttgccaac gtcaacgttg agatcgcgga
     1381 tgccattcac gatctctcgg tgttcgatca gtcgcggatc gaccgggcga tgattgagct
     1441 cgatgggacg ccaaacaaat cgcggctcgg cgccaatgcg atcctcggtg tgtcgttggc
     1501 agtagcacac gcagccgccg cgactgccgg tctgccgctg taccgctacc tcggcggacc
     1561 ctcggcaaac acgttgccgg tgccgatgct gaatatcctc aacggaggca agcacgccga
     1621 gaactccacc gatttccagg agttcatggt catgccgacc ggcgccgagt cgttcacccg
     1681 ggcgctgcag gtcgcttccg agatctacca gtcgttgaaa tcggttctac acaggcgggg
     1741 gctgaacacg aacgtcggcg acgaaggcgg cttcgcgccg tccttaacgg acaacgccgg
     1801 tgcgattgac ctcatcctgg aagccattga atccgctggg taccgggccg gagaggatgt
     1861 gcatgttgcc ctcgaccctg ctgcttccga gctgtacgtc gacggaaaat acaagctgac
     1921 gcgcgaagat cgcggcttgg agagcggcgc attggtcgcc ctctggaccg actgggtgag
     1981 gcgctacccg atcgtgagca tcgaagacgg catgggtgaa gacgattggg agggttggcg
     2041 gctactgacc gaacggattg gagaccgcgt ccaggtcgtc ggagacgacc tctatgtcac
     2101 caacacagaa cgcctcgccc gcggcatcaa ggagcgttcc agcaacgcga ttctgatcaa
     2161 gctcaaccag atcggtacgc tcactgagac gcttgctacg atcgagatgg cgaaacacgc
     2221 cggtttcgcg gccgtcgtga gccaccgcag cggcgagacc gaagatacga cgatcgccga
     2281 cctggtggtt gcgacgaatg ccggacagat caagaccggc gcccctgccc gcggcgagcg
     2341 cgtcgcaaag tacaaccggt tgctgaagat cgaacaggaa ctcggaccgg cggctaagta
     2401 tcccggcatg ggtgcgttct acaacatcga ccgctcgatt cggtaatcgt ggacgccgtg
     2461 cggcagccgt tccaacgcgt ctcaccggtt atatttcccg agcactggga cttgccgggc
     2521 aagccggcac agggtccggc tgcctcgctg gactcgtacc gccaaacgga tttccagctc
     2581 aagcacgacc tgcacctgtt gcacgagggc atgaacctgc agcttcgggt ggttgcggac
     2641 agctatcccg cgaagttccg cacgcatgca gccgcggcgt tccagctgta ctggtcacgt
     2701 gtgttccact acctgagcga ttccgcgctg ttggtgagcc gcggttcata cgtgggagta
     2761 ccgccgctga tgcggacggc ttgcgagtgc gtcgccgcgg ccgttcagct cggcggcgat
     2821 gaacttcccg cgttactcga tttcctggaa acaagcctcc gtcccgacga agcccaccgc
     2881 gcaaccgaag tcggccgtgg cagctaccac gccggcggaa cattggcgag cgacgcacgc
     2941 ctgggcctgg tctatcgcac gagcaccgaa ctggcgcgtc cacactttgg cgcgacgctg
     3001 atcgaagtcg cgccggaatc caacatccag aagctcgcgg taacgtttgg ggaccaggca
     3061 ttccacttcg gctgggcgca actcgagctc ggatggttgc tgtcgttgtg cgcgctgatt
     3121 ctcgatttcg cggtcgcggc ggacgtgctc ggcacaaccg acgaaagccg cgcggcgatc
     3181 gaagcgttgg gggaacgagt agacgcggcg ctgaaacagc ctgaccgctg tcgggtcgaa
     3241 gaggtagacg ccggcatgga ccgccggttc ttgatcgtca acttccgccg gcagtccgct
     3301 ggggcgccgc gcaagctatt gctgtaaacg cggtcagcag ttgtcgaatt tgccgctgac
     3361 gagcggcttg atcgcgccgg tttcgaggtc gtagctgcac cggtcgagac cgaccagatc
     3421 cttgccgtac acgtggattt cgactgttga tcgatcgcta agattgtcca tctggtggat
     3481 ctcgtcgatc tccggcacca gcaacgaaac ttctccagga aggacgcggg tggtgcgttg
     3541 cttcaccagc cgggcgaagc cctcgcggtc gcggtcatcc acacggcgga aacgcgtttc
     3601 ttcgatcccg ttgccaacca cgccgatcat tccccacgtg tgatggtcgt gcgggcccgc
     3661 gtggtcgccc ggtccccaaa cgacggcgct gacaaacagc ccgggtccac gatgcagcgc
     3721 gtagctgccg tgattcggtc gcctgccctt gcccgatgga cggcggtacg gcaacggtat
     3781 cgcctcggga tcctgcacca gccgctccag caaacgcgtg cctttctcga acaacagcgg
     3841 ctggtcgggg ctatcggcga tcagcgacgt catatcttga atgaagtctt caagcgagta
     3901 gcgagtaagg accattacgg gctcctttgg ccgattttct gcgccgtttc gaatgcggag
     3961 gacccaccaa ttataagctc atcacccaat gcgcggcgca ccaggcctac gtaaaccggc
     4021 aggtgtacta ccacgcttgc acgtcgcgcc gattccggtg gtaacgttat gtttgcggct
     4081 cgttcacgac accggaacgc agattctttt gccgtgcccg aggagtcgct tcatgacagc
     4141 cgaaggagag agaacgacgt ggcgacgaag atcggcatca acgggttcgg gcgcatcggg
     4201 cggcaggttc tgaaagcgat cattgaacgc catccggacg aactcgaggt cgtggcgctc
     4261 aacgatctcg tcgataccag catcaatgcc cacctgctga agcacgactc caactacgga
     4321 aacttcccag gcacggtcga aacgcgcgag gatgccctga tcgtcaacgg gcatacgatc
     4381 cgcgtgacca aatacccgga tccgggacag ataccctgga aggccatggg cgtgcaaatc
     4441 gtgatcgaat ccaccggccg tttcaccgac gccggcaagg cccgggcgca catcgacggc
     4501 ggcggagcca aaaaagtcat catctcggcg ccggccaaga atgaagacgt cacgatcgtg
     4561 ctaggcgtga atcaggacaa atacgacccc cgcaaagaca acatcatctc gaacgcctcc
     4621 tgcacgacca acgggctggc gccgatggtg aaggtgctgc tcgacaactt cggcatcgtc
     4681 aaggggcaga tgacgaccgt ccactcctac accaactcgc agaagatcct cgatctcgcc
     4741 tcggacgacc tgcgcgaagc gcgcgccggc ggcatgaaca tcgtgccgac gtcgaccggc
     4801 gccgctcgcg ccctcaggct cgtgatcccc gaagtcgatg gcaagatcga cggcctcgcc
     4861 tatcgcgttc cgaccccgac cgtctcgatc gtcgagatcg tggcgctcac cgaaaaggac
     4921 accacgaagg aagagatcaa cgccgcgatc tgcgaggcgg cggagggcaa gatgaagggc
     4981 atcatcggcc tgacgatgga gccgttggta tcgatggacc tcaaaggcga tgcgcggtcg
     5041 tcgatcgtcg acggtctttc gaccaacgtc gtcgccggca acctggtcaa ggtcgcatcc
     5101 tggtacgaca acgaatgggg ctactcctgc cgcctcgcgg atctgtgcgc cttcgtggcc
     5161 aagaaggggt tgtaggagca gcgagcaacg ggggttgagc gcccctactc cacctcgtct
     5221 ttcaacgccg gcaaggtgaa acggaacgcg gtgccgccgc cgcgccgagc ctcggcccag
     5281 atccggccgc ccatgatttt catgagcctt ctgcttaccg ccagaccgag gcctgcccct
     5341 tgtgacgccg tgcgcacggc atctgcagtc ctgtagaacg gctcgaagac gcgatccatg
     5401 tcggcagacg ggatgccgct tcctcgatcc agaacgctga acagcacctc ggcaccacgc
     5461 gcttccgccg agatttcgat gcgtttgccc ttgggcgaga actttaccgc gttctccaac
     5521 aggttccgaa gcacctgttc gaggaaggtt gggtcggctt ccaccaacgg caggtcggga
     5581 ggaacgtcga ccgtgagcgc gtggtttcct accctgccgc cgaggtcgag gatcaccttg
     5641 ttgacgaaac ggtgaacgat catcggctcg ggctgtaatt gcatccccgc ctgggcccgc
     5701 gcaagactca gaaggtcttc gacgaggtta tagagccgct cagcctcgtc ggccatgtcc
     5761 tgcaacagtt ggcggcgaac gtcgggttca agctggtcgc cccgcttgag caacgtcaat
     5821 gcgccgccgc gcaccgtcgt ggtcggcgtt cgcagttcgt gtgagacggt gttgatgaag
     5881 tcgtccttca atcgatcgac ttcacgcacc tgcgtgatgt cctggtaaac cgtaattacc
     5941 cctgagagcc gcccttccgc gtcgcgaatc gcggcagcgt tcacgagtac agggatctcg
     6001 cgcccggtac cggactgcct gacgatcagt tcttggacct gttcggcgct gccattgcgt
     6061 accgcccgtg cgagcggcgt ttccatcggg gtcaggagcc ggccttccgg cgtgaacagc
     6121 ccgaccatct cggcgtagcg gccgatctcg gtatcgaccg ggcgatatcc ccagatctct
     6181 tcgacagcac ggttgtaaaa gatgacccgc ccaccggcgt cgtacattgc cacgccggcg
     6241 gggagcgtct cgatcaacgt ctgcagccgt ttgcgctccg cttcagcgcg ttccaactgc
     6301 ctgattgcgt cgagttggcc gccgagctga gcgccgagcg tctcgaggaa ggtgcgctcg
     6361 tccgggctga attcgcgttt cacgcgtgcg ccgagcatta acacgcctgt gacggagttg
     6421 cggctgcgca acggcacatg gagaacggat accgtcccga gccgctttgc aagccgggcg
     6481 gaggcgctcc tgccccctcc gcgagcgaaa tccagcgcct ggatttcgct cgttgctatg
     6541 gcttcatcta ccccatagcc ggcacggagc ggcaacactc gtacagtctc gagtgcgtct
     6601 tcgtcaccgc cgcgggcggc caccatccgc agccgatcgc cgctcgcttc cagcagccac
     6661 acgcacgtca ggtcgacccc gggaaactga ggctcgagct cggcgagcag gttgcagcat
     6721 gcatcagcga cgtcttccgt ggccgcgagc gtttgcagcg cgtggttcaa cgccaggacc
     6781 cggtccgtgc gccgtcgctc ctccgtgatc cgctccgccg cgcgtaaaga atcgatcgaa
     6841 cccccaacgg taccggaaag caatgaagcg acgtcacgca gcgctgcgag ccgttcggcg
     6901 tcgaccttcc ccgctccgcc tcggaccgcg accacgagtg ccgcctgccc ttcccccgtt
     6961 tcgatgggct gcgcgagcaa cgtgctgccg ccgaggctgc tggagagcgg gccgattcgc
     7021 gcgtgccggt cccacaacct ccgagccagg gacctgcacg cggcctcctc ctccggcgag
     7081 agattgaaac tggtaagcgt tacctgtgcg tcgctgtctt tgcgtaggac gatcgcgccg
     7141 cgatcgcacc cgatcagtcg aagcgctgct tccagggagg agtcgagagg cgatgcgtgt
     7201 tgcggatctt tggtcgtaat caggcgtagc gggggacggt ccgccgtcat actgcgcctc
     7261 cgcgcgccgg ctgcccccgg ctcggacccc tggcgatgtg aggatagggt acgatgttga
     7321 tagcatcatg caggggcccc gtcaatcaga acggcggccg gcgtgcagca agaccgcaat
     7381 tcccaacaac gcaggaatag cgatgtttga gtcactgact gacaagttgc agggcgtatt
     7441 cggccgcctc aacagccacg gcacggtcac agaaaaggac ctcgaagagg cgatgcgcga
     7501 agtgcgcctg gcgctcctgg aagccgacgt caactacaaa gtcgtgcgcg aattcatcgc
     7561 ccaggtaaaa gaacgtgcgc tgggctccca aattctcacc agcctcacac ccactcaaca
     7621 gatcatcggc atcgtcaacg aagagctggt caagctgctc ggcagcgagc ccggacacat
     7681 taaccgcgct ccccagccgc ccacggtctt gatgctcgta gggctgcagg gctcggggaa
     7741 gacgacgcac tgcgccaagc tcgctctgca cctgcgccag cgcggcgaca agccgctgct
     7801 cgtcgccgcc gacgtctacc gcccggccgc ggtggaccag cttcaggccc tcggccgcca
     7861 gctcaatgtg ccagtgtacg cggagggcac cagcgtatcg cccgccacga tctgcgaaca
     7921 cgcgctggtc gaggcgaagc gtaccggcgc cacggtcgtg atcgtcgata ccgcaggtcg
     7981 tctgcacgtc gatgaagtaa tgatggcgga ggtcgtgcag ctacgcgact tgcttaagcc
     8041 gcaggaagtg tttttcgtcg ccgacgctat ggccggtcag gacgcggtca aagccgcgga
     8101 ggagttccac aaagcggtcg gtaccaccgg gctcatcttg acgaagctgg acggtgacgc
     8161 gcgcggcggc gccgtcttat cgatccgcgc cgtgaccggg gtcggagtaa agtttatcgg
     8221 catcggagag aagcccgacg cccttgacac gttctacccg gatcgcatgg cgcaacgcat
     8281 tcttggtatg ggcgacatcc aaacgctcgt cgaaaaggcc caggcagcga tgggcgacga
     8341 cgacgtcaaa gagctcgaac gcaagatgag gactgcgcag ttcgacctcc aggactttct
     8401 cacgcaaatg ggcaaggtca agaagatggg ccgggtcgcg gacctggtaa agatgattcc
     8461 gggtgttaac aagatcagct cacagcttgg cgtcgacgac atggacgacg gcttcttcaa
     8521 gaagacagag gcgatcatcc tgtcaatgac ggcggcggaa cgcaaaaatc cggggatcct
     8581 gcgtaaaggt cacagcgccg cgaaccgcaa acggcggatt gcaacgggct ctgccagcag
     8641 cgtaacggac gtgaatcagc tcttgaacca gttcgaagat gccaagaaga tgatgaaaac
     8701 tctcgccagc accaagggac gtggcttgct ggggagcatg ttccgttaag atgacagtgt
     8761 gtatttagcg ggcgcggccc tttgccgtgc cggaggtgat gtttgcttcg aatccgactc
     8821 cgtcgtgtag ggaagaaaaa ggcgccggca taccgaattg tcgtggccga tagccgcgca
     8881 ccccgcgacg gcgcgttcgt a
//
LOCUS       DATCPE010000091         7619 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U7219
            k141_85188, whole genome shotgun sequence.
ACCESSION   DATCPE010000091 DATCPE010000000
VERSION     DATCPE010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955033
            Sequence Read Archive: SRR1586279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 7619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 16:49:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 640
            CDSs (total)                      :: 631
            Genes (coding)                    :: 627
            CDSs (with protein)               :: 627
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7619
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85188"
                     /isolate="SMAG_U7219"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="VIM45_02705"
     CDS             complement(<1..215)
                     /locus_tag="VIM45_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEY8820229.1"
                     /translation="MPTISQLVRKGRAKVTKKTKAPALRFTYNALTNRTRRGDGSPQK
                     RGVCTQVRTVTPKKPNSALRKVARVRLT"
     gene            complement(530..1420)
                     /gene="ccsB"
                     /locus_tag="VIM45_02710"
     CDS             complement(530..1420)
                     /gene="ccsB"
                     /locus_tag="VIM45_02710"
                     /inference="COORDINATES: protein motif:HMM:TIGR03144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HEY8820230.1"
                     /translation="MHNLESFSYSLFWCGLVSVATASLLYLLATWGSRVVVHAAATSA
                     GTMTVAERQPLPPAVGRLATATSWVALAFLVSYLITRAMATGHPPYSDMFEYLSAFGT
                     LTTAFYVIFERRYRQRSLGIVAMPLALLLLLLAQTLFSPKIEPLVPALQNNRLLAIHV
                     ACMVISYSALTVAFAATVLYLVQTDANRYARLPKARVLETLGYRAVLVGFPLLGLGIA
                     LGAYWGNIAWGRYWGWDPKETTALVTWLIFAGFLHARSLAGWRGKRAALIVVAGWLMI
                     TFNIFAVNFWISGLHSYAGS"
     gene            complement(1447..2181)
                     /locus_tag="VIM45_02715"
     CDS             complement(1447..2181)
                     /locus_tag="VIM45_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HEY8820231.1"
                     /translation="MIASDVETEPAVEAPRPKRALGIFAHPDDAEFVCGGTVAKLCAA
                     GWEVRYIIATSGDKGTKDAALTPSKLAEMREEEQSAAGEVLGVKECIYLRYPDGFLEP
                     DVEFRGRLVRLIRRYRPDTVITWDGFRRGFNHRDHRAAGIATYDALFPAVRDPLYYPD
                     DLGDGLDYHKVGEILLAGSDQPDYFVDISDQLETKARAVYCHTSQIEAAPWDEFYKKY
                     MERARENGQSGGVPFSEAFRRVTMRT"
     gene            complement(2226..3551)
                     /locus_tag="VIM45_02720"
     CDS             complement(2226..3551)
                     /locus_tag="VIM45_02720"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY8820232.1"
                     /translation="MTNPTNRVDLEDARPGRASPRRVAVICYHSSPVVQPGGRDAGGM
                     NVYVRESSRELAARGWELDVFTRDDGNGPTVEVMSEGVRLIRVAAGPRRPVDKQKLYG
                     HIGEFACAIAAFRLSQGLRYDIVHSHYWLSGVAGLNLQRRWDTPHLTMFHTLGEVKNR
                     ARSGEREPAQRIDAERSIAASATRVICATGHERELLRSLYQAPEGRFATIPCGVDVDR
                     FKPLDKAAARRRLGFGDGGVLLYVGRLEPLKGIDIVIRAMAQLDAPRPLLVVAGGDQH
                     SATEVRRLRAQASAAGVEDRVRFVGAVPQEELPEYYAAADVCVIPSFYESFGMAALEA
                     QSCGRPVVASRVGGLPGVVRDGETGYLVPWRCPEPFAERLDVLLRNRALQRSFGAAAR
                     KWAMGFRWSSIAERLAALYEAMLTERALAGDCHPDHRVTPFPHVRCDVA"
     gene            complement(3548..4171)
                     /gene="folE"
                     /locus_tag="VIM45_02725"
     CDS             complement(3548..4171)
                     /gene="folE"
                     /locus_tag="VIM45_02725"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006896172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEY8820233.1"
                     /translation="MQSEQTRNGAGSLVKSGDQVGSVDQDRVKAAVAELIAAVGEDPS
                     REGLRGTPRRIADMYSEIFAGLFVDPRQVLSVNFEEGHDEMVILKDIPFYSLCEHHFL
                     PFHGVAHVGYIPDGSVVGISKVARVVELFAKRPQLQERLTGQIADCIMDALQPDGVAV
                     VVEAEHLCMTMRGVKKPGSRMVTSAMRGYFKKRAVTRAEFLSLVQGK"
     gene            complement(4202..5284)
                     /gene="thrC"
                     /locus_tag="VIM45_02730"
     CDS             complement(4202..5284)
                     /gene="thrC"
                     /locus_tag="VIM45_02730"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEY8820234.1"
                     /translation="MSTGLTKNNLVRQGVLFRYADRLPVTEATPFFSLGEGDTPLVKS
                     RRIVLEVGCEALYLKLEGCNPTGSFKDRGMVVAVAKAYEEGKRAIMCASTGNTSASAA
                     AYAAYLGMSCYVVIPMGNIASGKLAQAIAYGSTILAISGNFDDALRLVRELTRKHDIA
                     LVNSVNPHRIEGQKTAAFEIVDALGDAPDYVSLPVGNAGNITAYWKGFCEYAEFGRAT
                     RHPQMLGFQAEGAAPLVSGEPVEEPNTIATAIRIGRPATWAGAITARDESGGSIQAVS
                     DDEIVEAYRMLAREEGVFGEPASAAPIAGLLKRARAGENFAGKIIVSIVTGNGLKDVD
                     NAMKIPPQIFELPPDGAEIERALGWT"
     gene            complement(5259..6269)
                     /locus_tag="VIM45_02735"
     CDS             complement(5259..6269)
                     /locus_tag="VIM45_02735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8820235.1"
                     /translation="MSNERQASQVASTLAWLEDNVRETKAQIARLSQVAEEAQNQAWD
                     LAHRVIKAEEVAASLVTQLGMVPRIEGQVSSLSDRLVRVDDRQALVEAKLIEIGRQQQ
                     HDADYLRVELNEAVKRVDAGERLMQSWTVRLDTIEEIGRRSQDAASVVRQRIEEFERF
                     VDLVDQRVSRTSDALKRIDNEFTRINTEIEALQTQDALTGDRIQVYAEYIKRLDEQVA
                     IVGQQVDMRHEVTEKLELHRAGIRRGEERLGTLEASDEQTRQQADDLQRAIALIESKD
                     KALRDRLTELQEQLGQYNAHISDQFQKTHVLFEKQKRRLIENTERDIRELKVNVYRPD
                     EE"
     gene            complement(6278..7594)
                     /locus_tag="VIM45_02740"
     CDS             complement(6278..7594)
                     /locus_tag="VIM45_02740"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015327240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEY8820236.1"
                     /translation="MAHHNEDAHSIPVGLMGLGVVGGGVASILLDRLDVYARRIGRPL
                     ELRHVLVRDESKPRSVTLASGVITTDPRRILDDDAVEIIIEVMGGEHPALEYITEVLD
                     RGRSVVTANKEVMAKHGPRLLDLAQKRGVQLLYEASVGGGIPLIAPLKRDMLANELTS
                     VRAIINGTTNYILTRMSKEGADFGEALAAAQELGYAEADPTNDVEAFDAVYKLAILAG
                     LAFRTEVRPDQVFREGITRLTARDFRYAHELGYAIKLLAIGRRTEAGLEVRVHPAFIP
                     ADAPLAKVDGVFNAVQLEGDLIGTVLLQGQGAGSMPTTSAVVADVIDVARAIAQEGHP
                     ERLTWMRDAGVCIRPMSEVRTRFYLRVLVADQAGVLAKITRILGEKYDISIASIIQKE
                     TDEAAQTAELVIMTHEATEESVQGAIAEMSGLSVVAGIGNVIRVEG"
BASE COUNT         1389 a         2483 c         2257 g         1490 t
ORIGIN      
        1 gtgaggcgta cacgcgccac cttacgcaac gccgaatttg gcttcttcgg cgtcaccgtc
       61 cgtacttggg tgcaaacgcc ccgtttctgc ggcgaaccgt ctccccgccg cgttcggtta
      121 gtcagcgcgt tgtacgtaaa acgcagcgcc ggcgccttgg ttttcttcgt gactttagcg
      181 cggcccttac ggacgagctg gctaatcgtt ggcaagaaac tgtctcctga aagtcacagt
      241 gaaaggccgg atgccttcat ttctgagcca gttcggcgtg ggggcccgcc gattgcgggt
      301 tcctcactgg tgccgaccgt tgtcactttg gggccgtccg acctgcgtat gcggttagcg
      361 gtagtgtcga atacctagtt tacgaactgt ttggaagcgg tgtcaagcac gcacccttac
      421 cgtttgtccg tcgcggccac tcgcgcaggc agcgcatcgg ttccaacacc tttgaagcat
      481 accttaatcg gcgcgcttcg aggaaacccg tcgggctcat gtatcggcgc tatgagccgg
      541 catacgagtg gaggccggaa atccagaagt tgacggcgaa gatgttgaaa gtgatcatca
      601 gccagcccgc cacgacgatc agcgccgctc gcttgcctct ccaccccgcc aggctgcggg
      661 cgtgcaggaa gcccgcgaat ataagccacg tgaccagcgc cgtcgtctcc ttggggtccc
      721 aaccccaata acggccccag gcaatgttgc cccagtacgc ccccagagcg atgcccaacc
      781 ccaacagcgg gaagccgacc agcaccgcgc ggtagcccag cgtctccagc acccgcgcct
      841 tgggcaggcg agcgtagcgg ttggcatcgg tctgaaccag gtacagcacc gtggccgcaa
      901 atgcgaccgt gagggcactg tagctgataa ccatgcaggc cacgtggatc gccagcagtc
      961 ggttgttctg cagcgccggc accagcggct cgatctttgg cgagaacagc gtttgggcca
     1021 gcagcagcag cagtaacgcc agcggcatcg cgacgatgcc cagcgaccgc tgccggtagc
     1081 gccgctcgaa gatgacgtag aacgcggtcg tgagcgtgcc aaacgccgac aggtactcga
     1141 acatgtcgga ataaggcggg tggccggtcg ccatggctcg tgtgatgagg tagctcacca
     1201 ggaaggcgag ggcgacccac gacgtcgccg tcgccaggcg tcccaccgcg ggcggcaacg
     1261 gctgccgctc tgcgaccgtc atcgtgccgg cactcgtagc ggcagcgtgc acgaccaccc
     1321 gcgaacccca cgtggccagc aggtagagca gcgaggcagt ggcgactgag actaacccac
     1381 accagaacaa cgagtacgag aacgactcca agttatgcat cgatcgggcc cccgggtgcc
     1441 aatatcctac gttcgcatgg taacccggcg gaacgcttcg gagaacggga caccgccgga
     1501 ttgcccgttc tcgcgtgccc gttccatgta ctttttgtag aactcgtccc acggcgcggc
     1561 ttcaatctgg ctcgtatggc agtagacggc gcgcgccttg gtctcgagtt ggtcagagat
     1621 gtccacgaag taatcgggct ggtcgctacc ggccagcagg atctcgccaa ccttgtggta
     1681 gtccagccca tcgccgaggt cgtcagggta gtacagggga tctcgcactg ccgggaacaa
     1741 cgcatcgtag gtagcgattc ccgccgcacg atggtcgcgg tggttgaagc cgcgccggaa
     1801 accatcccag gtgattactg tatccggccg gtagcgccgg atgagccgca caaggcggcc
     1861 gcggaactcc acgtccggtt ccaggaaccc gtccgggtag cggagataga tgcattcctt
     1921 gacgccgagc acttcgcccg cggcggactg ctcctcctcg cgcatctccg ccagtttgct
     1981 gggagtgagc gctgcatctt tggttccttt gtcaccgctg gtggcaatga tgtaacggac
     2041 ttcccacccc gccgcgcaca gtttggcgac cgttccgccg caaacgaact cagcgtcgtc
     2101 gggatgcgcg aaaatcccca gggcgcgttt gggccgcggc gcctccaccg ccggctcggt
     2161 ctcaacatcg ctcgctatca tggcggcctc gcgtcaggta attacccagt tgcacggcga
     2221 gttccctacg cgacgtcgca gcggacatgc gggaacggag ttacgcggtg gtcgggatgg
     2281 cagtcgcccg caagggcacg ctccgtgagc atggcctcgt agagcgccgc aagcctttcg
     2341 gcgatcgaac tccaacggaa tcccatcgcc cacttacgcg cggccgcgcc gaaactgcgt
     2401 tgcagggcgc ggttgcgcag taacacgtcg agacgctccg cgaatggttc cggacagcgc
     2461 cacggcacca ggtaaccggt ctctccatca cggacaacac cgggcagccc accgaccctg
     2521 gacgccacaa ccggccgacc gcacgactgc gcctccaacg cagccatgcc gaagctctcg
     2581 taaaaggacg ggatcacaca cacgtcggcc gcggcatagt actcgggcaa ctcttcctgc
     2641 ggcacggcgc cgacgaaccg cactctgtcc tcgactccgg cggcggacgc ctgcgcacgc
     2701 agccgccgca cctccgtggc ggagtgctgg tccccaccgg ctacgactaa caatggacgt
     2761 ggcgcgtcca gctgcgccat ggcgcgaatg acgatatcaa taccctttag tggctccaac
     2821 cggcccacat acagcagcac cccgccgtcg ccaaacccga ggcggcgacg cgccgccgct
     2881 ttatctagcg gcttgaaccg atcgacgtct accccgcacg gaatcgtcgc gaagcgaccc
     2941 tctggggcct ggtagaggct ccgcaacagc tcgcgttcgt ggccggttgc gcaaatcact
     3001 cgcgttgcac tcgccgcaat cgatcgttcg gcgtcaatgc gttgggccgg ctctcgctca
     3061 cccgaccggg cgcggttttt cacctcgccc agcgtatgaa acatggtcag gtgtggcgta
     3121 tcccaccgac gttgcaggtt cagaccggcg actccagaaa gccagtaatg gctatgcacg
     3181 atgtcgtagc gcagtccctg actgagacgg aaagccgcga ttgcacatgc aaattcgcca
     3241 atgtggccgt acagtttctg tttgtccacc ggacgcctcg gccccgccgc cacgcgaatc
     3301 agacgcacac cttccgacat cacctctaca gtggggccgt taccgtcgtc gcgcgtgaat
     3361 acatccaact cccacccgcg ggcggcgagt tcgcggctgg attcgcggac gtatacattc
     3421 atcccgccgg catcgcgacc accgggctgc acgaccggag aagagtgata acagatgacg
     3481 gcgacgcgcc gcggtgacgc gcggcctggc cgagcgtcct ccaggtcgac ccggttcgtt
     3541 gggttagtca ttttccctgc acaagcgaga ggaattcagc gcgggtcacg gcccgtttct
     3601 tgaaatagcc gcgcatggcc gacgtgacca tgcggctacc cggctttttg acaccacgca
     3661 tggtcatgca caggtgctcg gcttcgacga ccaccgcgac gccgtcgggc tgtagcgcat
     3721 ccataataca gtccgctatc tggccggtca gcctttcctg aagttgcggc cttttcgcga
     3781 aaagctcgac gacccgcgcc actttgctga taccgacgac gctaccgtct ggaatatagc
     3841 cgacgtgcgc gacgccgtgg aacggcagga agtgatgctc gcaaagcgaa taaaacggga
     3901 tgtccttcag gatgaccatt tcgtcgtggc cctcctcgaa gttgacgctc aagacttgcc
     3961 ggggatcgac gaacagaccg gcgaagattt cggagtacat gtcggcaatc cggcgaggcg
     4021 tcccgcgcag cccttctcga gacgggtctt cgccaaccgc ggcgatcaat tcggctacgg
     4081 ctgccttcac gcggtcctga tcgacgctcc cgacctggtc ccccgattta acgagggagc
     4141 cggcgccgtt cctggtttgt tcggattgca tcagctttcg cgcaccccgc tatttcgatc
     4201 ctcaggtcca gcctagcgcc cgctcgatct cggcgccgtc gggcggaagc tcaaagatct
     4261 ggggcgggat cttcatcgcg ttgtccacat ccttgaggcc gttcccggtc acgatgctga
     4321 cgatgatctt gccggcaaag ttctcacccg ctcgggcacg ctttagcaat cccgctattg
     4381 gcgcggcgct tgcgggctcg ccgaaaacgc cctcttcgcg cgccagcatt cgatacgctt
     4441 cgacgatctc gtcatcggat acggcctgga tagacccacc ggattcgtca cgcgccgtta
     4501 tggcacccgc ccacgtggct gggcgtccta tcctgatcgc cgtcgcgatc gtgttgggct
     4561 cttcaaccgg ctcgccgctg acgagaggcg ccgcgccttc ggcctgaaat ccgagcatct
     4621 gcgggtgcct ggtcgcgcgg ccgaactcgg catattcgca gaagcctttc caatacgccg
     4681 tgatgttgcc cgcgttgccg accggcaggc tgacgtagtc cggcgcgtcg ccgagcgcat
     4741 cgacgatctc aaaggcggcc gtcttttgtc cttcaatgcg atgcgggttc accgagttga
     4801 ccagggcaat atcgtgcttc cgggtcagct ctcgcaccag tcgcaacgcg tcgtcgaagt
     4861 taccgctgat tgcgaggatc gtagatccgt aagcgatcgc ctgggcaagc ttccccgatg
     4921 cgatgtttcc catcgggatg accacatagc agctcatgcc aagataggct gcgtacgccg
     4981 ccgctgaggc gctagtgttg cccgttgacg cgcacatgat cgcgcgcttg ccttcctcgt
     5041 atgccttggc aacggcgacg accataccgc gatccttgaa cgaacccgtc gggttgcagc
     5101 cttcgagctt caaatacagc gcctcgcaac cgacctcaag gacgatgcgc cgcgacttca
     5161 ccagcggcgt gtcgccttca ccgagtgaga aaaatggagt tgcctcagta accggcagcc
     5221 ggtcggcata gcgaaaaaga acgccttggc gtacaagatt attcttcgtc aggccggtag
     5281 acattgacct tcagctcccg gatgtcccgc tcagtattct caatcagccg ccgcttctgt
     5341 ttttcgaaaa gaacgtgggt tttctgaaac tgatcggaga tgtgggcgtt gtattgcccg
     5401 agctgttcct gcaattcagt cagcctgtcg cgcaacgctt tatctttgct ttcgatgagc
     5461 gcaatcgccc tttgcaaatc gtccgcctgt tggcgcgtct gctcgtcgga agcttctaga
     5521 gtcccaagcc gttcttctcc gcgacgtatg ccggccctat gcaattcgag cttctcggtc
     5581 acttcgtgac gcatgtctac ctgttgcccg acgatcgcga cctgctcgtc aagccgcttg
     5641 atatattcag cgtagacctg aattcggtcg cctgtgagcg cgtcttgtgt ctggagcgcc
     5701 tcgatctcgg tgttgatacg tgtgaactcg ttgtcgatgc gcttgagggc atccgaggtg
     5761 cgggagacgc gttggtctac gaggtcgaca aagcgttcga actcctcgat ccgttggcga
     5821 acgaccgacg ccgcgtcctg tgatcgccgg ccaatctctt caatcgtatc aagccgcacg
     5881 gtccatgact gcatgagtcg ctcgcctgcg tccacccgct tcaccgcttc gttgagttcg
     5941 acgcggaggt agtcggcatc gtgctgctgc tggcgaccga tttcgatcag cttcgcctcg
     6001 accaacgcct gtcgatcgtc gacgcgaacg agtcggtcgg agagcgaaga gacctggcct
     6061 tcaatgcgtg ggaccatgcc gagttgtgtt accagcgatg cggctacttc ctcggccttt
     6121 atcacccggt gtgcgaggtc ccatgcctgg ttttgggctt cttcggcgac ctgcgagagg
     6181 cgcgcgatct gggctttggt ctcgcgaacg ttgtcctcga gccacgcgag cgtactagcg
     6241 acctgtgagg cctgacgctc gttgctcacc attgctgcta accctcgacc cgaatcacgt
     6301 tgccgatgcc ggccacgacg gaaagaccgg acatctccgc aatggcgcct tgtacagact
     6361 cttcggtcgc ctcgtgtgtc ataatcacca actcggcggt ttgagccgct tcatcggttt
     6421 ccttctggat gatcgaggcg atgcttatat cgtacttctc acccaagatc cgcgtgatct
     6481 tggccaggac tccggcctgg tcggcgacga gcacccgcag gtaaaaacgc gtgcgcactt
     6541 cgctcattgg ccgaatacaa actcccgcat cgcgcatcca tgtcaggcgt tcggggtggc
     6601 cctcctgcgc gattgcgcgg gcgacatcta tcacgtcggc cacgacggcg ctggtcgtcg
     6661 gcatcgagcc ggcgccctga ccctgcaaca gcaccgtgcc gatcaggtcg ccctcaagct
     6721 gaaccgcgtt gaacacgcca tcgacctttg ccagcggcgc gtccgccggg atgaaagcag
     6781 ggtgaaccct gacttcaagc ccggcttctg ttcgcctgcc gatggcgagc agtttgatcg
     6841 cataaccgag ctcgtgcgcg tagcggaagt cgcgcgccgt tagccgtgtg atcccttcac
     6901 ggaacacctg gtcgggccgc acttcggtgc ggaacgcgag gccggccagg atcgctaact
     6961 tgtatacggc gtcaaatgct tcgacgtcgt ttgtcggatc cgcctcggcg taaccgagct
     7021 cctgcgcggc ggcgagcgct tcaccaaagt cggcgccctc tttgctcatg cgcgtgagaa
     7081 tgtagttggt cgttccgttg atgatcgcgc gaaccgaggt cagttcattg gcgagcatgt
     7141 cgcgcttgag gggcgcgatc aacggaattc caccgccgac gctcgcttca tacagcaact
     7201 ggacgccccg tttttgcgcc aggtcgagga gtcttgggcc atgcttcgcc atgacctctt
     7261 tatttgccgt tacgactgaa cggccgcggt ccagcacttc cgtgatgtat tcgagggccg
     7321 gatgctcgcc acccatcacc tcgatgatga tctctaccgc gtcgtcgtcg aggatgcggc
     7381 gcggatccgt ggtaataacg cccgacgcga gcgtgactga gcgcggcttg ctctcgtcgc
     7441 gcacgagcac gtgccgcaat tccaatggcc ggccaatgcg gcgcgcatac acatcaagac
     7501 gatccagcag gatcgaagca acgccaccgc ccacgacacc caaccccatc aagccgacgg
     7561 gaatcgagtg cgcgtcctcg ttatggtgtg ccattgggca ccggcgggtt gccgctcgg
//
LOCUS       DATCPE010000092         3417 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U7219
            k141_119441, whole genome shotgun sequence.
ACCESSION   DATCPE010000092 DATCPE010000000
VERSION     DATCPE010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955033
            Sequence Read Archive: SRR1586279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 16:49:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 640
            CDSs (total)                      :: 631
            Genes (coding)                    :: 627
            CDSs (with protein)               :: 627
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3417
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119441"
                     /isolate="SMAG_U7219"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            120..656
                     /locus_tag="VIM45_02745"
     CDS             120..656
                     /locus_tag="VIM45_02745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8820237.1"
                     /translation="MNWPANKIESVAEPRATRIDLLHRPDIKTQIWMGGTDSLLGHRL
                     TLDQVEGAVVVDCCGDLPVDFQEGARLYLPRVFMDAEAVPYAYSRLVALAHGLATEIS
                     AQASHEAVIPPPDRIFVLCHYGMNRSGLFTGLLLRALGEDPVAAIDLIRRLRPGALSN
                     RTYVSLIERETCPLNEHR"
     gene            complement(684..1730)
                     /locus_tag="VIM45_02750"
     CDS             complement(684..1730)
                     /locus_tag="VIM45_02750"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY8820238.1"
                     /translation="MEIIEGVYQLVTPFPQFTYAQAREIRQDLGKKPRWIRSLPYVLP
                     YLIRSQGDLALVDNGWNTDVAHEALAQGMAEHAASPEELNQLIITHVHPDHFGLTGRL
                     WDESGAEVVMHEKEAEVIKSRYLSPQPLVDQMQNWMGRHGVPEMTAPELARGSMSMLD
                     KVSVREPDRVLQGGEHLKVGDFDLEVIWTPGHAPGHICLYEPNRKFLLSGDHVLPTIT
                     PNVSRHAQQTGNPLADYIKSLEKVEHLDVEILLPAHEFDTRDLKKRVAEIRAHHEERL
                     AEMERAIGEGATAWDVAGRVKWTTGMLAEFEPFMQRSAVGETLAHLEYLYELGRIRMH
                     EPANEGGVITWTRG"
     gene            1883..2461
                     /locus_tag="VIM45_02755"
     CDS             1883..2461
                     /locus_tag="VIM45_02755"
                     /inference="COORDINATES: protein motif:HMM:NF016149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopC"
                     /protein_id="HEY8820239.1"
                     /translation="MRFDRLLPLMALLGVFVTVSAAAAKAQYDHSSPGPGEVLSRQPA
                     RIDIYTRRPTSPVPGNTQVIVIDRDQRHVDSGDVVVDPTDHDHFSVGLQPGLPPGRYV
                     VTFKTLGETDLDHDGGEFAFYLGVQPDAAARAADRSLTLTVDDGDLTITGYRRGIIEG
                     GLTAIIAVPVALYYIQKRRRKAGEGASPEEPR"
     gene            2458..3093
                     /locus_tag="VIM45_02760"
     CDS             2458..3093
                     /locus_tag="VIM45_02760"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEY8820240.1"
                     /translation="MTPHWLSLRRSGGAVGLALVAMAVACGGGGKLKGTNLDATPAPD
                     FTLQDGGGARFSLSDYRGKAVLVTFLYTHCPDVCPAIAGKLSAADAKLGSARTRVAFV
                     AVSVDPDGDTPASVAQFTADHNLGSLGTRWRYGLGTKDELAAVWKDYFIGAEPEPAAV
                     RALHPGTPGPNVIDHDAAMYLIDAQGRERLLVRPDITIPDLVRDLKLLAGG"
     gene            complement(3094..>3417)
                     /locus_tag="VIM45_02765"
     CDS             complement(3094..>3417)
                     /locus_tag="VIM45_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8820241.1"
                     /translation="VSLKLNTATIVTDGERSCLRIPISAVWRDQLEQTIAALLVTAPV
                     EDEQRMAAIAHGVSELVRRWVERAVPVGASPEFLYPDREVRWPNEPESADATGQAVKE
                     ETAAT"
BASE COUNT          604 a         1089 c         1042 g          682 t
ORIGIN      
        1 gtcgaaaccg cgagccacga tcacgtcacc gcccatcagc agcagatcca cagctatgga
       61 tccgatccca ggatcccgca ttgcaaaggt tcgatgtcgg ctgtcgtcga gtccgccccg
      121 tgaactggcc ggcaaacaag atagaaagcg tcgcagaacc ccgcgccact cgcatcgatc
      181 tcctccaccg tcccgacatt aaaacgcaga tttggatggg cggtacggac agcctgctcg
      241 gccaccggct gacgctcgat caggttgagg gcgccgttgt cgtcgactgt tgcggcgacc
      301 tgccggtgga tttccaagaa ggggctcgcc tctacttgcc gcgggtgttt atggacgcgg
      361 aggccgtgcc gtacgcctat tcccggctgg tcgcgcttgc ccacggcctg gctacggaga
      421 tatcggctca agcgtcgcac gaagcagtta tcccgccgcc ggatcgcatc ttcgtcttat
      481 gtcactacgg aatgaaccgc agcggcctgt ttacagggct acttttgcgt gcgctcggag
      541 aagatcccgt agctgcaatc gatctgatcc gccggttgcg gcctggagcg ctgtcgaacc
      601 gcacgtacgt ctcgttaatc gagcgcgaga catgtccatt aaacgaacac cgttgacgca
      661 cgggatactc cgggaggccg acgttagccg cgggtccagg taatgactcc accctcgttc
      721 gcgggctcgt gcatgcgtat acgcccgagc tcatagaggt attcgaggtg ggcaagcgtc
      781 tcgccgacgg cagagcgctg catgaacggt tcgaattcgg caagcatgcc ggtcgtccac
      841 ttgacacgcc ccgcgacatc ccacgcagtc gcgccctcgc caatcgctcg ctccatctcg
      901 gccagccgct cctcgtgatg cgcgcggatt tcggccacgc gcttcttgag gtcgcgggtg
      961 tcgaactcgt gcgctggcag taggatctca acgtcgaggt gctcgacctt ttccagcgat
     1021 ttgatgtagt cggcgagggg gttgccggtc tgttgtgcgt ggcgtgacac gtttggcgta
     1081 atcgtcggca gaacatggtc accgctcaac aggaacttcc ggttgggttc gtacagacag
     1141 atatggcccg gggcgtgtcc tggcgtccag ataacctcca ggtcgaaatc gccaactttg
     1201 agatgttcgc cgccctgcaa gacacggtcg ggctcacgca ccgaaacttt gtccagcata
     1261 ctcatcgagc cgcgcgccag ttccggcgcc gtcatctccg gcacaccgtg tctgcccatc
     1321 cagttttgca tctggtccac cagaggctgc ggactcagat agcggctttt gatgacctcg
     1381 gcttcctttt catgcatgac cacttcagcg cccgactcat cccagaggcg gccggtcaac
     1441 ccgaaatggt ctggatggac gtgcgtaatg atgagctggt tcagctcttc ggggctggcg
     1501 gcatgctcgg ccattccctg ggcgagcgcc tcgtgcgcaa cgtccgtatt ccacccgtta
     1561 tcgacaagcg ccaggtcgcc ctggctgcga atcaggtagg gcaacacgta tggcagggac
     1621 ctgatccaac gcggcttctt gcccaggtcc tggcgaattt cgcgtgcctg cgcgtacgtg
     1681 aactgcggaa atggcgtgac aagctgatag acgccctcga tgatctccat gacctctccc
     1741 tatggtgtcg gcacgaaccc ggtgtcgttg ccgcacccaa tcctctttgg tttctatcac
     1801 cttgtcgacc tggcacgcca gcgcgcgcgg tgaacggcgc ctcgcagact gaagatcgat
     1861 cgatgagtac aataggttcg ccatgcgttt tgatcgctta ctgccgttaa tggcgctgct
     1921 cggggtgttc gtaaccgtca gcgccgcggc cgccaaggct caatacgatc attcgagtcc
     1981 cgggccgggt gaggttctgt cccgacagcc ggcgcgcatc gacatttata cgcggcgtcc
     2041 gacgagtcct gtgccgggca acacccaggt gatcgtcatc gatcgagacc agcgccacgt
     2101 cgattccggg gacgtggtcg tggatcccac cgatcacgat cacttttccg tggggctgca
     2161 gccaggactg ccgcccggtc gttacgtcgt caccttcaaa acccttggcg aaacggatct
     2221 cgaccatgac ggcggcgaat tcgcgttcta tcttggggta cagcccgacg ccgccgctcg
     2281 agccgcggat cgcagcctca cccttacggt cgacgatggc gatctgacca ttacgggata
     2341 ccggcgcggc atcatcgagg gcgggttaac ggcgatcatc gcggtgccgg ttgccctgta
     2401 ctacatccaa aagcgacgtc gtaaggcggg cgaaggagct tcacccgagg aaccgcgatg
     2461 accccgcact ggctttccct ccggcgttca ggcggtgcag tcggactggc gctggtggct
     2521 atggcagttg cctgtggcgg cggtggcaag ctcaaaggca cgaacctcga cgctacaccg
     2581 gctccggact ttacgctgca ggacggaggc ggcgcacgtt tctcgctcag cgactaccgt
     2641 ggcaaggccg tgctggtcac atttctgtac actcactgcc ccgacgtctg tccggcaatc
     2701 gccggaaagc tatccgctgc agacgcgaag ttgggctctg ccaggacccg ggtcgcgttc
     2761 gttgcggtca gcgtcgatcc ggacggtgat accccggcga gcgtggccca attcacggca
     2821 gaccacaatc ttgggtcgct cggtacacgc tggcgctacg ggctcggcac gaaggacgaa
     2881 ctggccgccg tttggaagga ttattttatc ggggctgaac ccgagcccgc ggcagtccgc
     2941 gcactacacc ctggaacgcc tggtcccaac gttatcgatc acgacgccgc catgtacctg
     3001 atcgacgctc agggccgcga gcggctgctg gtgcgccccg acatcacgat tccggacctg
     3061 gtgcgcgacc tgaaattgct agcgggcgga taattacgtc gccgctgttt cctctttgac
     3121 ggcctggccg gtcgcgtccg cggattcggg ctcgttgggc cagcgaactt cgcggtcggg
     3181 atagaggaac tccggtgaag cgcccaccgg gaccgcgcgc tcgacccagc gtcggactag
     3241 ttcagacacg ccgtgagcga tggccgccat tcgctgctcg tcctcaaccg gcgcggttac
     3301 cagcagcgcg gcgatggttt gctcgagttg gtcgcgccac acggcgctga tcggaatccg
     3361 gagacagctt cgctctccgt cggtgacgat cgtcgcggta ttcaacttga ggctgac
//
LOCUS       DATCPE010000093         2402 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U7219
            k141_573809, whole genome shotgun sequence.
ACCESSION   DATCPE010000093 DATCPE010000000
VERSION     DATCPE010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955033
            Sequence Read Archive: SRR1586279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 16:49:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 640
            CDSs (total)                      :: 631
            Genes (coding)                    :: 627
            CDSs (with protein)               :: 627
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2402
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_573809"
                     /isolate="SMAG_U7219"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            134..436
                     /gene="rplW"
                     /locus_tag="VIM45_02770"
     CDS             134..436
                     /gene="rplW"
                     /locus_tag="VIM45_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013985271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEY8820242.1"
                     /translation="MPKAIHPYAVLVRPLITEKGTMLGALDKYPFEVQIGANKIQVKE
                     AVELAFGVKVHAVNTLTVHGKTKRRGRQRVESTSRSWKKAIVTLKPGEKIELFAGV"
     gene            460..1287
                     /gene="rplB"
                     /locus_tag="VIM45_02775"
     CDS             460..1287
                     /gene="rplB"
                     /locus_tag="VIM45_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEY8820243.1"
                     /translation="MPIKQFKPTSPGRRGASGYTYEELTKKRPEKSLTVSLKRTGGRN
                     NQGRITTRHRGGGEKRLLRIIDFKRDKNGIPGRVAAIEYDPNRSARIALISYSDGDKR
                     YILCPVGLKVGETVQAGQGAEIKAGNAMSLRFIPTGTLVHNVELQLGRGGQIVRSAGT
                     AAQLMAKEGTYSLLRLPSGEMRRVLSECIATIGTVGNPEHNILKLGKAGRSRHVGRRP
                     TVRGSAMNPRDHPHGGGEGKAPIGMPGPKTPWGKPALGYRTRNNKATDKYIVRRRTR"
     gene            1350..1643
                     /gene="rpsS"
                     /locus_tag="VIM45_02780"
     CDS             1350..1643
                     /gene="rpsS"
                     /locus_tag="VIM45_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013240688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEY8820244.1"
                     /translation="MSRSTKKGPFVHESLLKKVDAVTRSGQKTVIKTWSRASTVMPQM
                     VGLTMAIHNGQKHVPIYITENMVGHKLGEFAPTRHFRGHTSKSEKTTRVAGKR"
     gene            1654..1986
                     /gene="rplV"
                     /locus_tag="VIM45_02785"
     CDS             1654..1986
                     /gene="rplV"
                     /locus_tag="VIM45_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEY8820245.1"
                     /translation="MRVQATARNIRVSPRKVRLLLNALPGKSIDDASTTLRYSGKPMA
                     KMVDKVLQSAAANAENNYNMDRDSLRVVEAYAGVGPTLKRFKPRSRGRVSPILKRSAH
                     ITVIVDEL"
     gene            2001..>2402
                     /gene="rpsC"
                     /locus_tag="VIM45_02790"
     CDS             2001..>2402
                     /gene="rpsC"
                     /locus_tag="VIM45_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015051614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEY8820246.1"
                     /translation="MGHKIHPTGFRIGIIYGWQSRWYADRTYTNLLHEDLAVRSSIMT
                     TLADASVSRVDIDRSANQMSVTIHTAKPGIVIGRQGAKVEELRSSLDKLTGRRVRVNI
                     QEIRIPELDSYLVARSVADQLQRRIAFRRAMK"
BASE COUNT          552 a          670 c          776 g          404 t
ORIGIN      
        1 cgtatgacgt gttgaaacac aagtatctgg tgctcacagt cgatgcagta cggcggatcg
       61 aagcgttgtg gggtggtcag cgggtgaata cgcggcagcc ggtctcagcc caaactccga
      121 cgggaggtgc ctgatgccaa aggccattca tccgtacgcg gttttggttc gtccgctgat
      181 caccgaaaag gggacgatgc tcggggcgct cgataagtac ccattcgagg tccagatagg
      241 cgcaaacaag atccaggtaa aagaggcggt cgagctggcg ttcggagtca aggtgcacgc
      301 tgtaaacacg ctcacggtac acggtaaaac taagcgccgt ggccgccagc gtgtggagag
      361 caccagccgc tcgtggaaga aagcgatcgt gacgctcaag ccgggcgaga agatcgagct
      421 gttcgcgggc gtctagcgca aagcaacgga gagagactgg tgccaataaa gcaattcaag
      481 cccacgtcgc cgggccgtcg cggcgcctca gggtatacct atgaggagct gacgaaaaag
      541 cgacccgaaa agtcgttgac cgttagcctg aaacggaccg gtggtcgcaa taaccagggc
      601 cgtatcacga cacgccatcg cggcggcggc gaaaagcggc tgctgcgcat catcgacttc
      661 aagcgcgaca agaacggcat tccaggacgg gtggctgcga tcgagtacga cccgaaccgc
      721 tccgcccgca tcgccctgat ttcgtattca gacggcgata agcgatacat tttgtgtccg
      781 gttggactga aggttggtga gacggttcag gccgggcagg gcgctgagat caaggccggt
      841 aacgccatgt cgctgcgttt cattccaaca gggacgctgg ttcacaacgt cgagttgcag
      901 ttgggacggg gcgggcagat cgtccgcagt gctggaactg cggcgcagtt gatggccaaa
      961 gaaggcacct acagcctgct caggctgccg tcgggtgaga tgcgccgcgt gctgtcggaa
     1021 tgcatcgcga cgatcggcac cgttggcaat cccgagcaca acatcctgaa actcggcaag
     1081 gccgggcgct cgcggcacgt cggacgccgg ccgacggtgc gcggttcggc gatgaaccca
     1141 cgcgaccatc ctcatggtgg tggcgagggc aaggcgccga tcggtatgcc cgggcccaag
     1201 acgccttggg gaaagccggc gctcggctat cgtactcgga ataacaaggc aacggataag
     1261 tacatcgttc ggcgtcggac gcgctaacga gcgcatcccg aacagggttg gcacgttgca
     1321 atccctgctc gtgaccagga ggtagaccct tgtccaggtc aacgaaaaaa ggaccgttcg
     1381 ttcacgaaag cctgttgaag aaagtcgacg cggtgacgcg gtccggccag aaaacagtga
     1441 tcaagacgtg gtcgcgggct tctacggtga tgccgcagat ggtcgggctc acgatggcga
     1501 ttcacaacgg ccagaagcac gtgccgatct acatcactga aaatatggtc ggacataagc
     1561 tcggtgagtt tgcgccgacg cggcacttcc gcggccacac gagcaagtcc gagaagacga
     1621 cccgcgtcgc cggcaaacgg taggacatac acgatgcgag tacaggctac agcaaggaac
     1681 atccgggttt cgccgcgcaa ggtgcgactg ctgctgaacg ccctaccggg caaaagcatt
     1741 gacgatgcga gcacaacgct tcgctattcg ggcaaaccga tggcaaagat ggtggacaaa
     1801 gttctgcagt cggctgcggc gaacgcggaa aacaactaca acatggaccg cgattcgttg
     1861 cgggtggtgg aggcgtatgc cggggtaggg ccgacgttga agcgattcaa gcctcgctcg
     1921 cgcggccgtg tcagcccgat tctcaagcgc tccgcccata tcaccgtgat agttgacgaa
     1981 ctgtaagagg agacacgcgc atgggtcaca aaatccaccc aacgggattt cgcatcggca
     2041 tcatttacgg ctggcaaagc agatggtatg cggaccgaac ctacacgaac ctgttacacg
     2101 aagacctggc ggttcgcagc agcatcatga cgactctagc cgacgccagc gtatcccggg
     2161 tggatatcga ccgcagcgcc aaccagatga gcgtgaccat ccatacggcg aagccgggca
     2221 tcgtcatcgg gcggcaaggc gccaaagttg aggagttacg ctcgtcgctc gataagctga
     2281 cgggccggcg ggtgcgggtc aacatccagg agatccgtat tccggagctt gattcgtacc
     2341 tggtggcgcg cagtgtcgcc gatcagttgc agcggcgtat cgcgtttcgc cgggcgatga
     2401 ag
//
LOCUS       DATCPE010000094         3346 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U7219
            k141_476561, whole genome shotgun sequence.
ACCESSION   DATCPE010000094 DATCPE010000000
VERSION     DATCPE010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955033
            Sequence Read Archive: SRR1586279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 16:49:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 640
            CDSs (total)                      :: 631
            Genes (coding)                    :: 627
            CDSs (with protein)               :: 627
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3346
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_476561"
                     /isolate="SMAG_U7219"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1215
                     /locus_tag="VIM45_02795"
     CDS             <1..1215
                     /locus_tag="VIM45_02795"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HEY8820247.1"
                     /translation="SVTHTGGSSFPGTTGAVIEPGDTIKTDSKGRAALQLPDGTVMRM
                     AGDTQITLTSAHFAKSGNLHDVSIFQKVGRTLTNVQHLIGGATFKVAGQSAVASVRGT
                     KFEVVMNTDGTMVVKLFTGLLHLEGKNPVDLVAPQQATVDAKGNVGPAGPILPDPNDP
                     FGPQAAASDQTSQGTTPGTEQDFIGAPIHNGEQQSFTYSFAGNGIVKAALGYNGSLMA
                     LRVKAPDGTVYTHQGPPPVVVLVPTAPAGIYTITVVGISGLGTAGEEPFVSVASLEPC
                     ATATVAQNGAVRHGYTPQDLANAISVPGLSNLSLSFAGDSVAGGIITGTGTYNGVSWS
                     GTVVLFWHGGVLEIFPVGASVFNVSVPATQIVQQIGAAIAQDPTNINPGYKVDRLFTC
                     QGVLMIDGRIGP"
     gene            complement(1410..2324)
                     /locus_tag="VIM45_02800"
     CDS             complement(1410..2324)
                     /locus_tag="VIM45_02800"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY8820248.1"
                     /translation="MSSNGNGQHVGGYRVLREIETDSLGVVYEAEHPRLHRKVAVRTI
                     EASVARDVSFSRRFMLELRSYVALEHQAIPRLYELAYDGDCPYLVTEMINGRTLDAVF
                     GDGVRYEPLGVIGLLRPIASALDYAHKRAAVHRDVKPANILLADDGRTLLTGFGLGMV
                     ASFNTVPGTGGPVAPDYVAPERLVGREVDGRADVYSLAAIIFEAVTGRRPFSSKSWIE
                     TLSRRLYEPPPSVRESAPELSAPFAAVLQQAMDREPDRRPATAGELLDRLERALTGKG
                     NDPSPAGLLARAKQRLPRRSRRRPAQPS"
     gene            complement(2380..2465)
                     /locus_tag="VIM45_02805"
     tRNA            complement(2380..2465)
                     /locus_tag="VIM45_02805"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2430..2432),aa:Leu,seq:tag)
     gene            complement(2490..>3346)
                     /locus_tag="VIM45_02810"
     CDS             complement(2490..>3346)
                     /locus_tag="VIM45_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012594079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEY8820249.1"
                     /translation="AHIDPGYFSVPQAIEFPTEGGLTAHAFYYPPKNKDYEGPPGERP
                     PLVVHSHGGPTSAAGSSFDLEFQYWTSRGFGIVDVNYGGSSGYGRAYRLRLNGNWGVV
                     DVDDCVNAARYLVGRGLADGKRVAITGGSAGGFTTLVALTKRNFFNAGASHYGIGDLV
                     TFVKDTHKFESRYLDTLVGPYPERADLYRERSAVNFADDLNCPVILFQGLEDKIVPPS
                     QAEEFVAVCERKKLPYAYLPFEGEQHGFRQAKNIRRSIEGELYFYSQIFDFATADEIE
                     PVDIQNFG"
BASE COUNT          627 a         1074 c         1038 g          607 t
ORIGIN      
        1 tcagtcacac ataccggggg gtcatctttt cccggcacga ccggggccgt gatcgagccg
       61 ggggacacga tcaagaccga tagcaagggc cgggcagcgc ttcagctgcc agacgggacc
      121 gtcatgcgta tggccggtga cacgcagatc acacttacgt ccgcccattt cgccaagagc
      181 ggcaaccttc acgacgtctc catctttcag aaggtcggcc ggaccttgac caatgtccag
      241 catctcatcg gcggggcgac tttcaaggtc gctggccaat ccgctgtcgc ttcagtacgc
      301 ggcacgaagt tcgaggtcgt catgaatacg gacggcacca tggtggtcaa gcttttcacc
      361 ggcctgctcc acctggaggg caagaaccca gtcgacttgg tcgcgccaca gcaagcgaca
      421 gttgacgcca agggcaacgt cgggcctgcc gggccaatcc tgcctgatcc aaacgatcca
      481 ttcggtccac aggcggccgc gtccgaccag acgagccagg gcacgactcc gggtactgag
      541 caggacttca tcggcgcgcc aattcacaac ggcgagcagc agtcctttac gtatagcttc
      601 gccggcaatg gcatcgtcaa agcggcgctc ggctacaacg gcagcttgat ggctctgcgt
      661 gtcaaggcgc ccgatggcac cgtgtacacc caccagggcc ctcctccggt tgtcgtcctc
      721 gtgcccacag cgccggccgg catttacacg atcactgtcg tcggtatcag cggtttaggc
      781 acggcgggcg aggaaccatt tgtgagcgtc gcctcgctcg agccctgtgc gacggcgact
      841 gtggcccaga acggagcggt tcgtcacggc tacacgcctc aggacctcgc caatgcaatc
      901 agcgtgcccg gcctgtcgaa tctcagcctg agcttcgccg gcgattcggt ggccggcggc
      961 atcatcaccg gcacggggac ttataacgga gtgagctgga gcggcaccgt cgtcctcttc
     1021 tggcacggcg gagtgctcga aatctttccc gttggagcga gtgtcttcaa cgtgagcgtc
     1081 ccggcgaccc agatcgtcca gcagatcggt gcggccatcg cacaggaccc gaccaacatc
     1141 aaccccggct acaaggtcga tcgcctgttc acctgccagg gcgtcttgat gatcgatgga
     1201 cgaatcgggc cctaacgggc tcgatgagag ggaaccagac cgttccctct catcgccggg
     1261 tcggcttcgc cgacgcggct gctctccttc ccgggattgg atttatttac ggattggtct
     1321 ctccacgccc cgattgggag tggtagaaaa caaagtcatg gccgagggga cccggccaac
     1381 cctgccgagt tagtgacgca ggcgcgacac tagctaggtt gagcggggcg acggcggctt
     1441 cggcgtggta gtcgttgctt cgctcgcgcc agcaggccgg cggggcttgg atcgttgccc
     1501 tttccggtca gcgcgcgctc gaggcggtcg aggagctctc cggctgtcgc aggccggcgg
     1561 tctggttcgc ggtccatcgc ctgctggagc acagcagcaa atggagccga gagctcaggc
     1621 gccgactcgc gcacgctggg gggcggctcg tacaggcgcc ggctcagagt ttcgatccag
     1681 ctcttcgacg agaagggccg gcggccggtg accgcctcga agatgatcgc ggccagcgaa
     1741 tagacatccg cgcgaccgtc gacctcgcgt ccgaccaatc gctcgggtgc aacgtagtcg
     1801 ggcgccaccg gcccgcccgt tccgggaacc gtgttgaacg aagcgaccat gcctaggccg
     1861 aagcccgtca aaagagtgcg gccgtcatcc gcaagaagaa tgtttgcggg cttcacgtcg
     1921 cgatgaacag ctgcccgttt gtgggcgtaa tcaagcgcac tcgcgatcgg ccgcagcaat
     1981 ccgattacgc ccaacggctc atatcggacg ccgtcgccga agaccgcgtc gagcgtacgc
     2041 ccattgatca tctcggtcac gagatacggg cagtcgccgt cgtacgcaag ctcgtagagg
     2101 cgcgggatgg cctggtgctc gagggcgacg tacgagcgga gctcgagcat gaaccggcgc
     2161 gagaagctca catcgcgagc gacgctggcc tcgatggtcc gcactgcaac ctttctatgt
     2221 aggcgcggat gctccgcctc gtagacaacg ccgaggctgt cggtctcgat ctcgcgcagg
     2281 acgcggtatc cgcctacgtg ttgaccgttg ccattgctgc tcatgtcaac ccgctcgcca
     2341 tgaaacctga actctaggcc accttacagg catcgaggct ggtgtcggcg agaggattcg
     2401 aacctccacg ccctttcgga caccagctcc taaggctggc gcgtctgcca ttccgccacg
     2461 ccgacatccc agagattatt taccctcgct catccgaagt tctgaatatc aaccggctcg
     2521 atctcatccg cagtcgcgaa atcgaagatc tgcgagtaga agtacagctc gccttcgatc
     2581 gagcgcctga tgttcttggc ctggcggaaa ccgtgctgct cgccctcgaa cggcaaatac
     2641 gcgtagggca gcttcttcct ctcgcacacg gcgacgaact cctcggcctg gctcggcggc
     2701 acgatcttgt cttcgagtcc ctggaaaagg atcaccgggc agttgaggtc gtcggcgaag
     2761 ttgacggcgg atcgttcgcg ataaaggtcc gctcgctccg gataaggccc gacgagagta
     2821 tccagataac gcgattcgaa cttgtgcgtg tccttgacaa aggtgacgag gtcaccgatt
     2881 ccgtaatggc tcgcgcctgc gttgaagaaa ttacgtttcg tgagcgcgac cagcgtcgtg
     2941 aatccgcctg ccgagccgcc cgtgattgcg actcgcttgc cgtcggcaag gccgcgcccg
     3001 acgagatagc gagcggcatt cacgcagtca tcgacgtcga caacgcccca gttgccgttg
     3061 aggcgcaacc ggtatgcgcg cccataaccg gatgagccgc cgtagttgac atcgacgatg
     3121 ccaaacccgc gactggtcca gtactgaaac tcgaggtcga agctggagcc ggccgcgctg
     3181 gtcggaccgc cgtggctgtg caccacgagt ggcggccgct cgcccggcgg cccttcatag
     3241 tccttgttct tcggcgggta gtagaaggcg tgagccgtca gaccgccctc ggtcggaaac
     3301 tcgatcgcct gcggcacgct gaagtaaccc ggatcgatgt gcgccg
//
LOCUS       DATCPE010000095         2960 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U7219
            k141_89889, whole genome shotgun sequence.
ACCESSION   DATCPE010000095 DATCPE010000000
VERSION     DATCPE010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955033
            Sequence Read Archive: SRR1586279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 16:49:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 640
            CDSs (total)                      :: 631
            Genes (coding)                    :: 627
            CDSs (with protein)               :: 627
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2960
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89889"
                     /isolate="SMAG_U7219"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(349..696)
                     /locus_tag="VIM45_02815"
     CDS             complement(349..696)
                     /locus_tag="VIM45_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8820250.1"
                     /translation="MKVAIVGSRHFSEPDRVSDYVGALPHGASIITGSASGVDAAATR
                     TARAKGIPVQVIPASFDELADPKKSAARNQRLIDACDVLVAFWDGASKGTRNTVERAL
                     DSGKEVHVFLGKG"
     gene            complement(701..2251)
                     /gene="pckA"
                     /locus_tag="VIM45_02820"
     CDS             complement(701..2251)
                     /gene="pckA"
                     /locus_tag="VIM45_02820"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HEY8820251.1"
                     /translation="MIGRVPARERPAPERRSLSPAALYEHAIRRDEAVIVSTGALTAE
                     TGQHTGRSPKDKFFVKEPTSQEAIWWVANQPIAPDKFDGLLQRVDEFCDSHEVFTQDV
                     FACADPRYRLRVRVVSELAWHSLFARNLFIRPNADELLNFEPDLTVVSVPSVKADPKR
                     DGTRSETFILVNLARRIVIIGGTEYAGEIKKSVFTALNYFLPAQGVFPMHCSANVSSG
                     GSDVALFFGLSGTGKTTLSADPSRRLIGDDEHGWSDSGVFNFEGGCYAKTIRIHKESE
                     PEIYAATEHFGTILENVVYDPRTRIPDYDSEKLTENTRAAYPVDLIPNAVLSGTGKHP
                     SNVIFLSADAYGVLPPVARLDEKQIGHYFLSGYTAKVAGTERGVTEPEPIFSTCFAAP
                     FLVLPPKRYADMLIERVNRHHAEVWMLNTGWVGGPYGVGQRMSIAHTRAIVRAVVQGK
                     LHGMPTHVDPVFGLSIPDQVPDVPHEVLDPRTSWPDPEAYDRQAGKLRAMFEKNILTI
                     GKSASTSG"
     gene            complement(2285..>2960)
                     /locus_tag="VIM45_02825"
     CDS             complement(2285..>2960)
                     /locus_tag="VIM45_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY8820252.1"
                     /translation="VREGPNVPVKMAAKDFVVHRTEHVTQASTVLMIDMSRSMFLRGC
                     FLAAKKVAIALDSLIRSQYPRDSLYVVGFSNYAVELKPQTLPQLALNDYVYGTNMQHG
                     FQLARSLLAKHRGNRQIIMITDGEPTAHLEDNGRAYFAYPPTFKTIQQTLREVRRCTR
                     DRVVINTFMLERGPYLTEFINQMTRINKGRAFFVSPDRLGEYVLVDYVSGKQRRRASS
                     KRSRIA"
BASE COUNT          516 a          923 c          968 g          553 t
ORIGIN      
        1 caggatgcag cttctggagc gcttcgtaga cgttggaaac tgtcgatccg gccggaagct
       61 cgacgttctc ggcgtcagtg ccggcctgct cacgcagccg ggcgaagagt cgcgcgcgca
      121 ccgcgatcgc gacagccatc acctaaatcc tagccacacc cgcactcgca aactctgccc
      181 accagcatcg cagggttggc cggggtcccc ccggccatga ctttgtccta acgatgctaa
      241 tcgcggcgag gagaaaaccc gtctaaaaca gagcccatcc ttataccgga gggagagcag
      301 gcgcgcgcga agcgcccgcc gatgagggga accaccgggg gttccctctc atccttttcc
      361 gagaaatacg tggacctctt tccccgagtc gagagcccgc tccaccgtgt tgcgcgtgcc
      421 cttcgaggcg ccgtcccaga aggcgacgag gacatcgcag gcgtcgatca gccgctggtt
      481 gcgagccgcg gacttcttag gatcggcaag ctcatcaaac gaggctggga tcacctggac
      541 cgggatgccc ttggcgcggg ccgtcctggt cgccgcggca tcgacgccgc tggcgctgcc
      601 ggtgatgatc gaggcgccgt gcggcagtgc gccgacgtag tcgctcactc ggtcgggttc
      661 agagaagtgc cggcttccca cgattgccac cttcacggcg tcagccggat gtcgatgcgc
      721 tcttgccgat cgtgaggatg ttcttttcaa acatcgcacg gagcttgccg gcctgccggt
      781 cgtaggcttc ggggtccggc cagctggttc gcgggtccag gacctcatga ggaacgtctg
      841 gcacctggtc gggaatcgac aggccgaaca ccggatcgac gtgagtcggc atgccgtgca
      901 gcttgccctg aaccacagct cgcacgatcg cgcgtgtgtg tgcgatcgac atgcgctgtc
      961 caactccata gggcccgccg acccagccgg tgttcagcat ccagacctcg gcgtgatgcc
     1021 ggttgaccct ctcgatcagc atgtcggcgt accgcttggg gggcaggacg aggaacggcg
     1081 cggcgaagca agtgctgaat atcggctccg gctcggtcac cccgcgctcc gtgcctgcca
     1141 ctttggccgt gtaaccagag aggaagtagt ggccgatctg cttctcgtcg aggcgcgcca
     1201 ccggaggcag aacgccgtac gcgtccgcag acaggaagat gacgttgctc gggtgcttgc
     1261 cggtccccga gagcaccgcg ttcgggatca ggtcgacggg gtaagcggct cgagtgttct
     1321 cggtgagctt ctcggagtcg taatcgggaa tacgcgtgcg cgggtcgtag acgacgtttt
     1381 cgaggatcgt gccgaaatgc tcggtggccg cgtagatctc cggctccgac tccttgtgga
     1441 tcctgatcgt cttcgcgtag cagccgcctt cgaagttgaa gacgccggag tcactccagc
     1501 catgctcgtc gtcaccgatc aggcggcgcg atggatcggc ggagagcgtg gtcttgcccg
     1561 tgccgctcag gccgaagaac agcgccacgt cggagccacc gctgctgacg ttcgcggagc
     1621 agtgcattgg aaacacgccc tgtgccggca gaaagtaatt gagggcggtg aaaacggact
     1681 tcttgatctc tcccgcgtac tcagtgccgc cgatgatgac gatgcgacgc gcgaggttga
     1741 ccaggatgaa ggtctcgctt cgcgtcccat cgcgcttggg gtcggccttc accgatggca
     1801 cggacacgac cgtgaggtca ggttcaaagt tgagcagctc gtcagcgttc ggccggatga
     1861 aaaggttgcg ggcgaagagg ctgtgccacg cgagctcgct cacgacgcgc actcgcagcc
     1921 ggtaccgcgg atcggcgcag gcgaaaacgt cctgcgtgaa cacctcatgg ctgtcgcaga
     1981 actcgtcgac gcgctgcagc agcccgtcga acttgtcagg cgctatcggc tggttggcca
     2041 cccaccagat cgcctcctgg ctggtgggct ccttgacgaa gaacttgtcc ttcggtgagc
     2101 ggcccgtatg ttgcccggtc tcggcggtga gggcgccggt ggacacgatc acggcttcgt
     2161 cgcgcctgat cgcgtgctcg tagagagcag ccgggctcag gctgcggcgt tcgggcgcgg
     2221 gtctctccct ggcgggcact ctaccgatca tagggaagtg gccatagggc ccgtcggaga
     2281 cggtctaagc gattcgagac cgtttgctgc tggcgcgccg ccgctgcttt ccactgacgt
     2341 agtcgaccag cacgtattcg ccaagccgat ctggcgagac gaagaacgcc cggcccttat
     2401 tgatgcgcgt catctggttg atgaactccg tgagataagg cccgcgctcg agcatgaacg
     2461 tgttgatgac cactcggtcg cgtgtgcacc gcctcacctc gcgcagcgtc tgctggattg
     2521 ttttgaaggt cggcggatag gcgaagtacg cccgcccatt atcctcgaga tgagcggtcg
     2581 gctcgccgtc cgtgatcatg atgatctggc gatttccgcg gtgcttcgcc agcagtgagc
     2641 gtgcgagctg gaagccgtgc tgcatgttgg tgccatagac gtagtcgttg agcgcgagct
     2701 gcgggagcgt ctgcggcttc agctcaacgg cgtagttgga gaaacccacc acgtacagcg
     2761 agtcgcgcgg gtactggctc cgtatcaagg agtcgagcgc gatcgccacc ttcttggccg
     2821 ccaggaagca gccccgcaga aacatcgacc ggctcatgtc gatcatgagg acggtcgaag
     2881 cctgcgtgac gtgttccgtg cgatggacca cgaagtcctt cgccgccatc ttcacgggca
     2941 cattcggccc ctctcgcacg
//
LOCUS       DATCPE010000096         1607 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U7219
            k141_122791, whole genome shotgun sequence.
ACCESSION   DATCPE010000096 DATCPE010000000
VERSION     DATCPE010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955033
            Sequence Read Archive: SRR1586279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 1607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 16:49:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 640
            CDSs (total)                      :: 631
            Genes (coding)                    :: 627
            CDSs (with protein)               :: 627
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1607
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122791"
                     /isolate="SMAG_U7219"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..476)
                     /locus_tag="VIM45_02830"
     CDS             complement(<1..476)
                     /locus_tag="VIM45_02830"
                     /inference="COORDINATES: protein motif:HMM:NF013828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8820253.1"
                     /translation="MRRAVEFGHPDFGRAIPCVCVLTEHEDERQERLQRFSNLGALAR
                     QSFATLLPRGRSPEASHQERFARALADAVRYAEDPQGWLVFVGESGTGKTHLAAAIAN
                     QCIEHGVPVFFTVVPDLLDHLRAAYGPASEIPYDRLFEVVRNAPVLVMDGLGTQFST"
     gene            complement(553..1308)
                     /locus_tag="VIM45_02835"
     CDS             complement(553..1308)
                     /locus_tag="VIM45_02835"
                     /inference="COORDINATES: protein motif:HMM:TIGR01446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HEY8820254.1"
                     /translation="MNAGGSPSSINDRVPENRGPARQGGFPAGARQTAIPSVFFSDVL
                     PAIDDPAELLVTLYAFYLLSRRRGSRRWFDEAELAAEPPFMRSLAKVNPDSAVALRGG
                     LDAAVNRGSLLRAVRHRDGRSLRLYAVHMPGAAEMLASLDAMVHRPVDGAGGADRQDP
                     LPNIFVLYEENIGVISPLLADQLGTAEADYPWPWIEAAFREAVALNRRSWRYIERILE
                     RWRTEGPDLEAIRRSAEGGRKSLAGRYWRLVKR"
     gene            complement(1322..>1607)
                     /locus_tag="VIM45_02840"
     CDS             complement(1322..>1607)
                     /locus_tag="VIM45_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HEY8820255.1"
                     /translation="RAVESRTPHIPMLSDLRESGSIEQDADVVMFIYREDAYMRKDDW
                     QDTHPDQPGRSYPTGIASIIIAKHRNGPTGQVDLRFHNRTTKFEDLQVTD"
BASE COUNT          293 a          523 c          488 g          303 t
ORIGIN      
        1 gtactgaact gtgtcccgag tccgtccatc accaggaccg gcgcattgcg cactacttca
       61 aatagacggt cgtacggtat ctcgctcgcc ggtccatacg ccgcgcgcag gtgatcgagc
      121 aggtctggca cgaccgtaaa aaagaccgga accccgtgct cgatgcattg attggcgatc
      181 gcggccgcca ggtgggtttt gccggttccg ctctccccca cgaacacgag ccacccctgc
      241 gggtcttctg cataccgtac ggcgtcggcg agtgcgcggg cgaaccgctc ctgatgcgat
      301 gcttcgggac tgcggccgcg aggcagcaac gtcgcgaagc tctgtcgagc cagcgcgcca
      361 aggttgctga accgctgcaa gcgctcctgc cgttcgtcct catgttcggt gaggacgcag
      421 acgcatggaa ttgcgcgacc aaagtcaggg tggccaaact ctaccgcacg gcgcacgaac
      481 cctgcgcctg agcactgggg gcagttcgga tcccccgaac cgcgttcgcc gaccagaaac
      541 gtggccggaa gatcagcgtt tgacgaggcg ccagtatcgt cccgccaggc tcttccgccc
      601 gccctcagca gatcttctaa tcgcttcaag gtccggtccc tcggttcgcc agcgttcgag
      661 aatacgctca atataccgcc aactgcggcg gttcagcgcc acggcctcgc gaaaggccgc
      721 ctcgatccag ggccagggat aatccgcctc tgcggtgcca agttgatccg ccagcaacgg
      781 tgagatcacg ccgatattct cttcgtacaa cacaaaaatg ttgggcagcg gatcttgcct
      841 gtccgcgccg ccggcgccat caaccggacg atggaccatc gcgtcgagcg aagccagcat
      901 ctccgcggca ccgggcatgt gaactgcgta taaccggagg gatctgccgt cgcggtgtct
      961 gacggcccgc aacaacgacc cacggttcac ggcggcgtcg agaccgccgc ggagcgcaac
     1021 cgccgaatcc gggttcacct ttgccagcga acgcatgaat ggcggctcgg cggccagctc
     1081 tgcctcgtcg aaccagcggc ggctgccccg gcggcggctc aacagataga aagcgtacag
     1141 ggtgacgagc agctcggccg ggtcgtcgat cgccggcaac acgtcgctga agaacacgct
     1201 cggtatcgcc gtctggcgag cgccggcagg gaatccgccc tgtcgcgccg gtccgcggtt
     1261 ctcgggaact cggtcattaa tactgcttgg cgatccgccg gcgttcatgc gctgtttacc
     1321 atcagtcggt gacttgcagg tcttcaaatt tcgttgtgcg attgtggaat cgcagatcta
     1381 cctgccctgt cggtccattc ctgtgcttgg cgatgatgat cgacgcgatg cccgttgggt
     1441 agctacgtcc cggttggtcc ggatgagtgt cctgccagtc gtccttccgc atgtaggcat
     1501 cttcgcgata gataaacatg acgacgtcgg cgtcctgttc gatgcttcca gattcacgca
     1561 ggtcgctcag catagggatg tgcggcgtac gagattccac cgctcgc
//
LOCUS       DATCPE010000097         4930 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U7219
            k141_514845, whole genome shotgun sequence.
ACCESSION   DATCPE010000097 DATCPE010000000
VERSION     DATCPE010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955033
            Sequence Read Archive: SRR1586279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 4930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 16:49:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 640
            CDSs (total)                      :: 631
            Genes (coding)                    :: 627
            CDSs (with protein)               :: 627
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4930
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_514845"
                     /isolate="SMAG_U7219"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /locus_tag="VIM45_02845"
     CDS             <1..556
                     /locus_tag="VIM45_02845"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEY8820256.1"
                     /translation="DGYNRLKEETVQSERIYEGKILNLRVDTVRFDDGRQAKREVVEH
                     GGAVAIVPVDRNGNIVLVRQFRGPVGDELLEVPAGMVNVDEDPDAAVQRELQEETGFR
                     AERIRRLTGFWVAPGYTTEFINVYLAEGLVESPLGADEDEAIDVETYPLSEALAMVDR
                     GEIRDAKSIVGLLSLARERAEQCV"
     gene            616..1389
                     /locus_tag="VIM45_02850"
     CDS             616..1389
                     /locus_tag="VIM45_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8820257.1"
                     /translation="MATEVVKARRPAAATERHDLWWLLPATVVVVLTAFVVYTTWAAL
                     DNNNFYHSPYLSPFYSPCLSSNCGKAVNWSIFGSWWWLSPAIFILAFPGGFRLTCYYY
                     RKAYYRSYFLSPPACGVADAAKGYQGERRFPFILQNVHRYFFYIVWVILAFLWYDAFE
                     AFRFDEGWGIGLGSLIMVANVVLLTLYSFSCHSCRYLSGGYLDRFFKAPTRYQMWRAV
                     SRLNAKHANFAWFSLFSVVITDVYIRLVASGTISDWRIV"
     gene            1389..3191
                     /locus_tag="VIM45_02855"
     CDS             1389..3191
                     /locus_tag="VIM45_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HEY8820258.1"
                     /translation="MVAESTYETHDYDVIVVGAGGAGLRAAIECSAQGAKTALICKSL
                     LGKAHTVMAEGGIAAALGNVWPEDNWKVHWRDTMRGGKLLNNWRMAQIHAQEAPDRVL
                     ELEEWGALFDRTPEGLILQRDFGGHRYARLAHVGDRTGLEMIRTLQQHAVHKGIDVFM
                     ECTIHRLLKDKDRIAGAFGYWRESGRFVLFKSKAVVLATGGIGKAYKVTSNSWEYTGD
                     GDTLAFDAGADLMDMEMVQFHPTGMVWPPSVRGILVTEGVRGDGGTLKNSEGVRFMFN
                     YIPDFFRAETADTEEEADAWYADKKNRRTPDLLPRDEVARAINSEVKAGRGSPHGGVF
                     LDIASRRDADYIRRRLPSMYHQFKELANVDITAGPMEVGPTCHYMMGGIRVDADTAAT
                     TVPGLFAAGEVAAGLHGANRLGGNSLSDLLVFGRRAGLYAAEYAGGLRTSPVVDGGEV
                     EVCAQVMLAPFDNSGDENPYSIQADLQECMHNNVGIIRTEVELKLALEQIAVLRDRSR
                     RVRVEGNRQYNPGWHLALDLHSLLTVSEAITRAAIERKESRGGHTRDDYPTTDAKFGG
                     VNIVLRKRDGELTLGQELLPQIPDDLKQLLEETT"
     gene            3191..3952
                     /locus_tag="VIM45_02860"
     CDS             3191..3952
                     /locus_tag="VIM45_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HEY8820259.1"
                     /translation="MGDGFTMRLWRGDAASGAFKEYNVDVGEGMVVLDVVHRVQAELA
                     PDLAVRWNCKAGKCGSCSAEINGKPRLMCMTRMDTFEANSTITVSPLRTFPLIKDLVT
                     DVSFNFEKAKEVPAFKPKPKEADGTRRMYQPEVDRVQEFHKCIECFLCQNVCHVIRDH
                     DENKGSFAGPRLFVRYAALEMHPLDTNDRLELIRQKAGLGFCNITKCCTEVCPENIHI
                     TDNAIIPLKERVVTEYYDPVVWLARKVTGRGKNRG"
     gene            complement(4022..>4930)
                     /gene="mdh"
                     /locus_tag="VIM45_02865"
     CDS             complement(4022..>4930)
                     /gene="mdh"
                     /locus_tag="VIM45_02865"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HEY8820260.1"
                     /translation="GATMALLLAQKGYADIVLQDIVEDMPQGKALDMLEAGPIEGYDV
                     SIIGTNGWKETANSDVIIITSGVPRKPGMTRDDLLKVNMGIVKSVAENAAKASPNAIF
                     VVFANPMDAMCHVVKDVSDFPKQRIIGQGGVLDTSRFRTFIAEELGVSMQDVQAYVLG
                     GHGDTMVPLTKYTTVGGVPVENLISKDRLAEIVQRARDGGAEIVRLLKTGSAFYAPAA
                     ATIEMVDAILLDRKRILPCAAYLEGEYGVKNLFVGVPVKLGATGIEQVVEIPLTGDEK
                     VALQRSADAVRELVEAMDRIKQESPA"
BASE COUNT          965 a         1436 c         1560 g          969 t
ORIGIN      
        1 cgatgggtac aaccggttga aagaagagac cgtacagagc gagcgaatct acgaagggaa
       61 gatcctcaac ctgcgggtcg acacggttcg cttcgacgac ggcaggcagg cgaagcgcga
      121 ggtcgtggag cacggaggcg ctgtcgcgat tgtaccggtg gaccgcaacg gtaacatcgt
      181 actcgttcgc cagttccgag ggccggttgg cgacgagctg ctggaggttc ctgcggggat
      241 ggtgaatgta gatgaggacc ctgacgcggc cgtgcaacgt gagttgcagg aggagaccgg
      301 gttccgggcc gagcgcatcc gccggctgac gggcttttgg gtggctcccg gctatacgac
      361 cgaattcatc aatgtgtacc tggctgaagg gctggtcgaa agcccgctcg gtgccgacga
      421 ggatgaggca atcgacgtgg aaacctaccc tctttctgaa gcgctggcaa tggttgaccg
      481 cggcgaaatc cgcgatgcaa agagcatcgt aggcctgctc tccctcgcgc gagagcgggc
      541 ggagcaatgc gtctagctga tcgctcgacg gtcacaacgg ctccaaaaat cagccgaaat
      601 cgaggaaggt gaacgatggc aacggaggtt gtgaaagcaa ggcggccggc ggccgcgacg
      661 gagcggcatg acctgtggtg gctgctaccg gcgacggtcg tcgtggtgct gaccgcgttc
      721 gtcgtctaca cgacgtgggc ggcgctcgac aacaataact tttatcactc tccgtatctt
      781 tcgcccttct attcgccttg cctatcaagc aactgcggca aggcggtcaa ctggtctatt
      841 tttggatcgt ggtggtggtt gtcgcctgcc attttcattc tagcgttccc gggtggcttt
      901 cgcctcacct gctactacta ccgtaaagca tattaccgct cgtacttctt gtcaccgccc
      961 gcctgcggtg ttgccgatgc ggccaagggc taccagggag aacgacgctt tccattcatt
     1021 ctgcagaacg ttcatcgcta cttcttttac atagtctggg tgatcctcgc atttctgtgg
     1081 tacgacgcat ttgaggcatt tcgtttcgac gaaggctggg gaattggctt gggcagcctg
     1141 atcatggtcg ctaacgtggt cctgttgacg ctgtatagct tctcgtgtca ttcctgccgc
     1201 tatctgtcgg gcggctatct ggatagattc ttcaaagcgc caaccaggta ccagatgtgg
     1261 cgcgccgtca gccggttgaa tgcgaaacac gcaaacttcg cctggtttag cctgtttagc
     1321 gttgtgatca ccgatgtgta cattcggctg gtggcttcgg gcactatcag tgactggagg
     1381 atcgtctaat ggtcgccgag agcacgtacg aaacccacga ctacgatgtc atcgtggtcg
     1441 gggccggtgg cgccggtttg cgcgcggcga tcgagtgctc tgcccaaggg gcgaagacgg
     1501 ctctcatctg caagtcgcta ctgggcaagg cgcacaccgt catggccgaa ggcgggatcg
     1561 ctgctgccct cggtaacgtt tggccagagg acaactggaa ggtccattgg cgcgatacca
     1621 tgcgcggcgg caagctgctc aacaactggc gcatggccca gatccatgcc caggaggcgc
     1681 ccgaccgcgt gctcgagttg gaagagtggg gggcgctctt cgatcgcaca ccggaagggc
     1741 ttatcctgca gcgcgacttc ggtggacatc gctacgcgcg gcttgcacac gtaggtgaca
     1801 gaaccgggct ggagatgatc cgcacgttgc agcaacacgc ggtccataag gggatcgatg
     1861 tcttcatgga atgcacgatc caccgcctgc tcaaggacaa ggaccggatc gctggagcct
     1921 tcggttattg gcgggagagc ggtcggttcg tgctgttcaa gtcaaaagca gtggtccttg
     1981 ccaccggcgg tatcggcaag gcgtacaagg ttacgtccaa ctcgtgggag tacaccggtg
     2041 acggcgatac gctcgcattc gacgcgggtg ccgacctgat ggacatggag atggtgcaat
     2101 tccatcccac gggcatggtc tggccgccca gcgtgcgcgg catcctggtg accgaagggg
     2161 tgcgtggcga cggcggaacg ctgaagaact cggaaggcgt ccgcttcatg ttcaactaca
     2221 tccccgactt ctttcgagca gagaccgcgg acaccgagga ggaagcggac gcctggtacg
     2281 cggacaagaa gaacaggcgc acgccggatc ttttgccgcg cgacgaagta gcgcgggcga
     2341 tcaactccga ggtcaaagcc gggcgtggct cgccgcacgg aggcgtcttc ctggatatcg
     2401 cctcgcggcg cgatgctgat tacatccgca ggcgcctgcc atcgatgtac caccagttca
     2461 aagagctggc gaacgtggat atcacggcgg gaccaatgga ggtcgggcca acctgtcact
     2521 acatgatggg aggaatccgc gttgacgccg ataccgccgc tacgaccgtg cccggcctgt
     2581 ttgccgcggg cgaggttgcg gccggcctcc acggtgccaa ccggctgggc ggcaactccc
     2641 tatccgacct gctggtcttc ggtcgtcggg cggggctgta cgcggcggaa tacgcgggcg
     2701 gcctgcggac ttcgccggtg gtagacggcg gcgaggtcga agtgtgcgcc caggtgatgc
     2761 tcgccccatt cgataactct ggcgacgaga acccctattc gatccaggcc gacctgcagg
     2821 agtgcatgca taacaacgtc ggcatcatcc gcactgaagt ggagctgaag ctggccctcg
     2881 agcagatagc ggtccttcga gaccgatccc ggcgtgtgcg ggtggaaggc aaccggcaat
     2941 acaaccccgg ctggcacctg gcgctggacc tccactcttt gctcaccgtc tcggaagcga
     3001 ttacgcgcgc ggcgatcgag cgcaaggaga gccggggtgg ccatacacgg gacgactacc
     3061 cgacgaccga cgcgaaattc ggtggcgtga acatcgtcct gcggaagcgc gacggcgaat
     3121 taacgcttgg ccaggagctc ctgccccaga taccggacga tctcaagcag ctcttggagg
     3181 agacgacgta atgggcgacg gtttcacgat gcgtctctgg cgcggtgatg ccgcgagcgg
     3241 cgcgttcaaa gagtacaacg tcgacgtcgg cgagggcatg gtcgttctgg acgtcgtcca
     3301 tcgcgttcag gcagaactgg cgcctgattt ggctgtgcgt tggaactgca aagccggaaa
     3361 atgcggctcg tgcagcgccg agatcaacgg taaaccgcgg ctgatgtgta tgacgcggat
     3421 ggatacgttc gaggccaaca gcaccatcac agtgtcgccg ctgaggacct ttccgctgat
     3481 caaggatctg gttacggatg tctcgttcaa cttcgagaag gcaaaggaag tgcccgcttt
     3541 caagcccaag cccaaggaag ccgatgggac acggcgcatg taccagccgg aggtagatcg
     3601 ggtccaggag ttccacaagt gtatcgagtg ttttctgtgc cagaacgtct gccacgtcat
     3661 ccgtgaccac gacgaaaata aggggagctt cgcgggtccg cgtttgtttg tacgatacgc
     3721 ggcgctcgag atgcatccgc tcgacaccaa cgaccggctt gagctcatca ggcagaaggc
     3781 cggtctggga ttttgcaaca tcaccaagtg ctgcaccgag gtatgccccg agaacatcca
     3841 catcacggac aacgccatca tcccgctgaa ggagcgagtt gtaaccgagt actatgaccc
     3901 ggtcgtctgg ttggcgcgca aggtcaccgg ccgcggcaaa aatcgaggct gaagaagatc
     3961 aaaagggggc cggccatcgt agccagcccc ctttgtatcg agcatcaaac atgctttggt
     4021 tctaggccgg gctctcctgc ttgatccggt ccatggcttc gaccaactcc cgcacggcgt
     4081 cggcagatcg ttgcagcgcc accttctcgt ccccggtcag cggaatctcc accacctgct
     4141 cgatgcctgt cgctcccagc ttcaccggca caccgacaaa caggttcttg acgccgtact
     4201 cgccctccag gtacgccgcg catgggagga tgcgcttgcg gtcgagcaga atggcgtcca
     4261 ccatctcgat cgtcgccgcg gccggtgcgt agaaggcgct accggtcttg agcagccgca
     4321 cgatctcagc accgccatcg cgggcgcgct gcacgatctc ggcaagcctg tcctttgaaa
     4381 tgaggttctc gacagggacg ccgccgacgg tcgtgtactt ggtgagcggg accatggtat
     4441 cgccgtgtcc gccgagcacg taagcctgta cgtcctgcat cgagacaccc aactcttcgg
     4501 cgatgaaagt gcgaaagcgg cttgtgtcaa gcacgccccc ctgacctatg atgcgctgct
     4561 tcgggaagtc gctgacgtct ttgacgacat ggcacatcgc gtccattggg ttggcgaaaa
     4621 cgacgaagat cgcgttggga gatgctttgg cggcgttttc agccacgctc ttgacgatgc
     4681 ccatgttgac cttcagcagg tcgtcgcgcg tcattcccgg cttccgcggc actcccgacg
     4741 tgataatgat cacatcggag ttagcggtct ctttccagcc gttggtgccg atgatgctta
     4801 cgtcgtatcc ctcgatcggg ccggcttcga gcatgtcgag cgctttgccc tgcggcatat
     4861 cctcgacaat gtcctgcagc acgatatctg cgtagccctt ttgcgcgagc aagagggcca
     4921 tggtggcgcc
//
LOCUS       DATCPE010000098         2138 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U7219
            k141_318422, whole genome shotgun sequence.
ACCESSION   DATCPE010000098 DATCPE010000000
VERSION     DATCPE010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955033
            Sequence Read Archive: SRR1586279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 16:49:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 640
            CDSs (total)                      :: 631
            Genes (coding)                    :: 627
            CDSs (with protein)               :: 627
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_318422"
                     /isolate="SMAG_U7219"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(415..1500)
                     /locus_tag="VIM45_02870"
     CDS             complement(415..1500)
                     /locus_tag="VIM45_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015793302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEY8820261.1"
                     /translation="MSMRFQHAKVCPSAAPGVARCHSLVRVDDFSQPLATSGPTGYGP
                     ADLRAAYTLASTTGVGQTIAIVDAFDDPNAESDLAVYRTQFGLGPCTTASGCFRKVDQ
                     NGGTAYPRGDMGWAEEISLDLDMASAICPNCKILLVEAKTNSFANLAIAEDRAATMSA
                     TVISNSYGGGEYSGEVNDQAHFNHPGIAITVSSGDGGYGVEFPAASQYVTAVGGTSLI
                     RDASARGFSETAWSGAGSGCSAFIAKPSWQTDAGCARRTVADVSAVADPNTGVSVYDT
                     YRLHPGGWLVFGGTSVSAPIIGAVYALAGNASSITYGSFPYAHLSSLFDVTSGSNGSC
                     GSSYLCTAVLGFDGPTGNGTPSGSGAF"
     gene            1944..>2138
                     /locus_tag="VIM45_02875"
     CDS             1944..>2138
                     /locus_tag="VIM45_02875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8820262.1"
                     /translation="MPIKDKVIQDFEFVDGGRKFYCSVEAPGQTGMPPWWWFRPDTET
                     TTRYAPFEASPTDTKRSVQTR"
BASE COUNT          427 a          629 c          696 g          386 t
ORIGIN      
        1 tgcgcaaacg attcgaacat aatgaccgac ctcggtggag gtgttccagc gttcccgctg
       61 atggcgcggg aggatagggg cctggtggga aaccagaaga aggcacgtat tactgcttcg
      121 agtctgacga gcgacgatat gtgcgggaaa cgcggtccgg aggctgtccg cgagggctcc
      181 cggaaacgga cgagttgacc gtctctgatc gccctttacc ggcttatggc cctcgtcgat
      241 aagacgagcg cggcgccgcg cgggagggaa gtggcagaaa cgaaatggcc acaaaccttg
      301 aggcccctcc acttcgttta ttgttgtgcc gtgcgtccag gtcggaaaag gaacgtgccc
      361 ggtcgaatcg accgggcacg ttgtgtgtac ctgaggaaca gaacctctta ggcgctagaa
      421 agctccactg ccactcggcg tgccgttgcc agtgggcccg tcgaagccca gcaccgcagt
      481 gcagaggtaa gagcttccgc agctgccgtt gcttcccgaa gtgacatcga agagcgacga
      541 cagatgagca tacgggaatg atccgtaggt gatcgaactg gcgttgccgg ccagggcgta
      601 gacggcgccg atgatcgggg ctgagacgct ggttccgccg aagacgagcc atccgccggg
      661 atggagacgg tacgtgtcat agaccgagac gccagtgttc ggatcggcga cggccgaaac
      721 atcggcaacg gtccgacgtg cgcagcccgc gtctgtctgc cagcttggct tggcgatgaa
      781 cgcgctgcac ccgctgccgg cgccgctcca cgcggtctcg ctgaaaccgc gagcgctcgc
      841 atcccgaatg agcgaagtcc cgccgaccgc ggtcacgtac tgtgaggccg ccgggaactc
      901 gacgccgtac ccgccgtcac cggagctgac ggtgatcgcg atgccagggt ggttgaaatg
      961 tgcctggtca ttcacctcgc cggagtactc accgccgccg taggagttcg agatgacggt
     1021 ggcgctcatc gttgccgccc ggtcctccgc gatggcaaga ttcgcgaagg agttcgtttt
     1081 cgcctcgacg agtaggatct tgcagttggg gcagatcgcg ctggccatgt cgagatcgag
     1141 cgagatctct tccgcccacc ccatgtcacc gcgcggatag gcggtgccac cgttctgatc
     1201 gaccttgcgg aagcagccgc tggcggtggt gcagggcccg agcccgaact gggtgcgata
     1261 caccgccagg tcgctctcgg cattgggatc gtcgaacgcg tcgacgatcg caatcgtctg
     1321 gccgacgccg gtagtactcg cgagcgtgta cgcggctcgc aaatcggctg gcccatatcc
     1381 ggtcgggccg ctcgtagcca gcggctggct gaagtcgtcg acgcgcacta aggaatggca
     1441 gcgggccaca cctggcgccg cgctcggaca gacctttgcg tgctgaaaac gcatcgacat
     1501 gccggtgttc ctatcggcat tgaccgatgc gtttggcccg gtgatggccg ccggcgggag
     1561 cgtgttctcg gaagagcacg ctgccaatgc agccaccgct gcgatgctaa ctacggtacg
     1621 agcgcggagc aacataatgg tgtctccatt gaaagatggt accgctcagt tacacatcct
     1681 gggcgctaga ggaagctgcg tcgacgcagc gatccttgcg gccctgacga gccgctctcc
     1741 ggacacgcga aagtagcgtc tgaaatgaac actttcaata ggcaggtctt ctcgacttga
     1801 ctgagtcggg ggcgggttgg ggatcggcaa atccggcgtc agcggctaat ccgcaatgct
     1861 ggatgccccc taataaagag cgaaaagtcg gaatacggcc aaagtcgacc gaatggctac
     1921 ccgtcatcta ggtagattgc atcatgccga taaaggacaa agtgatacag gatttcgagt
     1981 tcgttgatgg tgggcgaaag ttctactgtt cggtcgaagc acccggtcag acgggtatgc
     2041 cgccatggtg gtggtttcga cccgataccg agacgaccac gcgctacgcc cccttcgagg
     2101 cttcgcccac cgacacgaag cgatcagttc agacgcgg
//
LOCUS       DATCPE010000099         5833 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U7219
            k141_516133, whole genome shotgun sequence.
ACCESSION   DATCPE010000099 DATCPE010000000
VERSION     DATCPE010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955033
            Sequence Read Archive: SRR1586279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 5833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 16:49:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 640
            CDSs (total)                      :: 631
            Genes (coding)                    :: 627
            CDSs (with protein)               :: 627
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5833
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_516133"
                     /isolate="SMAG_U7219"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..348)
                     /locus_tag="VIM45_02880"
     CDS             complement(<1..348)
                     /locus_tag="VIM45_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8820263.1"
                     /translation="MTTDSAVPAAPLEGRWDLEATDVQGIERALVTLRKQYGSAAAAR
                     EGHAGTRNSVANLVIYAASDADAEQATATMSQLAGAHPSRTILLIAGLEPDEPGVRAS
                     VSAACHMDGAKRIC"
     gene            complement(364..1932)
                     /gene="zwf"
                     /locus_tag="VIM45_02885"
     CDS             complement(364..1932)
                     /gene="zwf"
                     /locus_tag="VIM45_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008477322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEY8820264.1"
                     /translation="MTTAQTTGRTEENGQRTNPLRAGQRGLRTAPPATMVIFGASGDL
                     TKRKLVPALYNLALEHHIPAEFTVLGVSRSECENDEFRKDMREAVDEFSRSGKARPAV
                     WDGFAQGLFYISGDTKNKETFKKLKAELERLDAERGTQGNRIFYLSTPPGIFQEIIEG
                     LGAAGLNKQDHGFSRVIIEKPFGSDLATAKELNDHIAKVFDESQVYRIDHYLGKETVQ
                     NLLVLRFANEIFEPVWSRNYIDHVQITVAESIGIEGRGGYYETAGALRDMLQNHLMQL
                     MTLVAMEPPASIDANGVRDEKVKVLRSIRPICNSDVVRDVVRGQYGPGWVAGDQVQGY
                     RQEKGVDLESTTETYVATRLFVDNWRWADVPFFLRTGKRMPRRVSEIAVQFKQAPHLA
                     FASQSITNFRPNVLALRIQPDEGMSLTIGAKVPGSVMRIRSVNMDFSYGASFLVEPPE
                     AYERLLLDCMLNDQTLFKRRDEVEEGWKTFQCVLDGWGVEEEPDFPNYEAGTWGPREA
                     DYLIERDGRHWRTP"
     gene            complement(1944..2843)
                     /gene="gnd"
                     /locus_tag="VIM45_02890"
     CDS             complement(1944..2843)
                     /gene="gnd"
                     /locus_tag="VIM45_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HEY8820265.1"
                     /translation="MQLGMIGLGRMGFNMTERLMLGGHKLVVYDRSPEAIQSAQDKGA
                     TGAVSLKELVAKLSAPRAVWIMVPSGAPVDQTIDELLPLMSKDDVIIDGGNSFYKDSI
                     ARGQRVAAAGLHYQDCGTSGGIWGLKNGYCLMIGGDREIFNRIEPIYKTLAPADGYGY
                     MGTSGSGHYVKMVHNGIEYGMLQAYGEGFELMNASDYGLDLQEISKIWNHGSVVRSWL
                     IELAELAFEREPHLDSLRGYVDDSGEGRWTVFDAIEKNIPAPVITLSLLRRLSSRQEE
                     SFSAKVIAALRNEFGGHAVKKES"
     gene            complement(3064..>5833)
                     /locus_tag="VIM45_02895"
     CDS             complement(3064..>5833)
                     /locus_tag="VIM45_02895"
                     /EC_number="2.2.1.2"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007080.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional transaldolase/phosoglucose
                     isomerase"
                     /protein_id="HEY8820266.1"
                     /translation="GVTANPTIFEHAVSAGNDYDADIRMLVAKGADANRIYERLATDD
                     VRSACDLLRPVYDRTNGADGFVSLEVSPLIATDTAATIKEVKRFWGIVDRPNLMVKIP
                     ATENGLPAIEQSLTDGININITLIFAVERYIEVANAFVRALRQRVLRGEPIDRIASVA
                     SFFVSRVDTLGDGLLEAKIAAAGSPEERKQLESLKGKAGIANAKIAYERFKQIFYGDE
                     FKDLRDRGARVQRPLWASTSTKNPAYRDVMYVEELIGPDTVDTMPPATIDAFRDHGVV
                     RNTIDEDVAEAHRFMERLAKVGIDMKKVTQQLEDEGVASFAKSFNTLADQIERKRGAI
                     RANLAQNQTASLGGLQARVDKALDAAAKNHVADRVMKKDATLWKPDAKPNDKSLSGWL
                     GWLAAIDDGLKAMDDLDSFVEQVRGEGYSDAVVLGMGGSSLAPLVMERTFGRRSGFLN
                     LHVLDSTDPANVLAIERSVNLAKTIFIVSSKSGSTTEPNTFFAYFWHKVRRSGAGDSG
                     KQFVAITDAGSSLNKLAEERKFRRVFHGDPEIGGRYSALSPFGLVPAALAGVDVRAIL
                     QRASTMMASCSATFVPRENPGVWLGTVLGGLAKAGHDKVTFLPSPSLDSFGLWGEQLL
                     AESTGKEGKGLIPIAGEDAGDPSVYGKDRVFVHIYLASDHDAGLERCVKALADAGQPV
                     IWLKLNDKKDLGAEFFRWEFATAVAGAILGINPFDQPNVQESKDNTVRLLNEFQLKGS
                     LPRPTTVAREDGVQLISMAASGNGKGSGARANGHDGAGGSVNRNVAAFLKQVKPGDYV
                     AIMAYLPYDEAIERELQRRRLALRDGLHVATTLGFGPRFLHSTGQLHKGGPNSGLFIQ
                     VTAKHAQDVPVPGEPYSFGTLEDAQALGDLQSLQKHGRRVIGLHIEGDVMAALAVLGP
                     EAVTLAQT"
BASE COUNT         1029 a         1936 c         1691 g         1177 t
ORIGIN      
        1 gcagatccgc ttggcgccat ccatatggca ggcagcagag acacttgccc gcaccccggg
       61 ttcatccggt tccaggcccg caatcagcaa gatcgttcgc gaagggtggg ccccagcgag
      121 ctgagacatc gtcgccgtcg cctgctccgc gtcggcatcc gaggcagcgt agattacgag
      181 gttggcaaca ctgttgcgtg tgcccgcgtg tccctcgcgc gccgcggctg cgctgccgta
      241 ctgtttacgc aacgtaacca gcgccctctc aatgccctgc acgtcggtcg cttcgagatc
      301 ccagcgcccc tcgagtggcg ccgccggaac agcggaatcg gttgtcactc ctaaccccca
      361 gccctacggc gtccgccagt ggcggccatc gcgctcgatc aggtagtcag cctctcgtgg
      421 accccaggta cctgcctcgt agttgggaaa gtcgggttcc tcttctaccc cccagccgtc
      481 gagcacgcac tggaaggtct tccagccctc ttcgacctcg tcgcgccgct tgaacagcgt
      541 ctgatcgttg agcatgcagt ccagcagtaa ccgctcgtac gcttccggtg gctcgaccag
      601 gaacgaggcg ccgtatgaaa agtccatgtt tacagaccga atgcgcatca ccgaccccgg
      661 caccttggcg ccgatcgtca gcgacatgcc ctcatcgggc tggatgcgaa gcgcaagcac
      721 gtttggacga aaattggtga tcgactgtga cgcgaatgcg aggtggggcg cctgtttgaa
      781 ctgcaccgcg atctcgctca cccggcgcgg cattcgctta ccggtgcgaa ggaaaaacgg
      841 cacgtcggcc cagcgccagt tgtccacgaa gagccgcgtg gcgacatacg tctccgtggt
      901 cgattcgaga tcgaccccct tctcctgccg gtagccttgc acctgatcac ccgcaaccca
      961 gccgggtccg tactggccac gcaccacgtc gcgcaccacg tcgctgttgc agatcggccg
     1021 gatggagcgc agcaccttca ccttttcgtc gcgcacgccg ttcgcgtcga tcgaagcagg
     1081 cggctccatc gccacgagcg tcatcagctg catcaagtgg ttctgcagca tgtcgcgcaa
     1141 cgcgccggca gtttcatagt agccgccacg cccttcgatc ccgatgctct cagcaacggt
     1201 gatctgcacg tggtcgatat agttgcggct ccagacgggc tcgaagatct cgttggcgaa
     1261 gcgcagcacc agcaggttct gcaccgtctc cttacccagg tagtggtcga tacggtaaac
     1321 ctggctctcg tcgaacactt tggcgatgtg gtcgttgagc tctttcgccg tcgcaaggtc
     1381 gcttccgaac ggcttttcaa tgattacgcg tgagaagccg tggtcctgct tattgagacc
     1441 ggccgcccca agcccctcga tgatctcctg aaagatgccg gggggcgttg agaggtagaa
     1501 aattcggttt ccctgcgtcc cccgctcggc gtcgagccgc tccaactcag ccttgagctt
     1561 cttgaatgtt tccttgtttt tggtatcgcc ggaaatgtag aagagaccct gggcgaaacc
     1621 atcccagacc gcgggacgcg ccttgccact gcgcgaaaat tcgtccaccg cctcgcgcat
     1681 gtccttgcgg aactcgtcgt tttcgcactc tgagcgcgag actccgagga cggtgaactc
     1741 tgccggaatg tggtgctcga gcgcaagatt gtaaagcgcc ggaactagct tgcgcttggt
     1801 gaggtcgccg gaggcgccga aaatcaccat cgtcgcgggg ggcgccgttc gcagcccgcg
     1861 ctggcctgcc cgcagcggat tggttcgctg tccgttctcc tcagtacggc cggtcgtttg
     1921 cgccgtcgtc atgctgcctc cgtctacgac tcctttttga ccgcgtgacc gccaaattcg
     1981 ttccgtagag cggcgataac ttttgcggag aacgactcct cctgccgcga agagagacgc
     2041 cgcagcagcg agagtgtgat caccggcgca gggatatttt tctcgatcgc atcgaacacg
     2101 gtccagcggc cttccccgga atcgtccaca tagcccctca atgaatcgag gtgcggctca
     2161 cgctcgaacg ccagttccgc cagttcgatc agccaggatc ggacaacgct tccgtggttc
     2221 cagatctttg agatctcttg gaggtcgagg ccgtaatccg aggcgttcat gagctcgaaa
     2281 ccttcgccgt acgcctggag catcccgtat tcgatgccgt tgtgcaccat cttgacgtag
     2341 tgtccggagc cgctcgttcc catgtacccg taaccgtctg ccggcgccag cgtcttgtag
     2401 atcggttcaa tgcggttgaa gatctcccga tcgccgccga tcatgaggca ataaccgttc
     2461 ttcagccccc agatgccgcc gctggtgccg caatcctgat agtgcaggcc ggcagccgca
     2521 acgcgctgcc cacgggcgat gctgtccttg tagaacgaat tgccgccgtc gataatcacg
     2581 tcgtccttcg acatgagcgg aagcaactcg tcgatcgtct ggtccaccgg cgctccggac
     2641 ggcaccatga tccagactgc acgcggcgcc gaaagcttgg ccacgagctc tttcagggaa
     2701 accgcaccgg tcgcgccttt gtcctgagcc gactgtatcg cctccggact gcgatcatag
     2761 acgaccagct tgtgcccgcc gagcatcaag cgctcggtca tgttgaaacc catacggcct
     2821 aacccgatca tgccaagttg cattcggtct ccccctagcc cttggattcc taacccccga
     2881 cccgatagac ctaacccccg accccttccc taaccaagga aggggagctt caactcgcta
     2941 gtttctagag cattccgtcc acttgtagtg gcgcatggta atgcgccctc tgcgacgctg
     3001 gaccaatgcc cgcgcctgac gccagtggat cgccccttcc cggctttagg gaaggggagg
     3061 gggttaggtc tgtgccagcg tcactgcctc cggacccagc accgccaacg ccgccatgac
     3121 gtcgccctca atgtgcagcc cgatgacgcg gcggccgtgc ttctgcagcg actgcaggtc
     3181 gcctaacgcc tgcgcgtcct ccagcgtgcc aaaactgtat ggctcgccgg gtacgggtac
     3241 atcctgcgcg tgcttcgccg tgacttggat gaataggccg gagttaggcc cgcccttgtg
     3301 cagctgaccg gtcgaatgca ggaagcgcgg accgaagccc agcgtcgtcg cgacgtgcag
     3361 gccgtcgcgc aacgcgaggc gtcgccgctg aagctcacgc tcaatcgctt cgtcgtaggg
     3421 cagataggcc atgatcgcca cgtagtcgcc cggcttcacc tgcttgagga acgcggcgac
     3481 atttcggttt accgagccgc ccgctccgtc atggccgttc gccctggcgc cgctaccctt
     3541 gccgtttccg ctcgcggcca ttgagatcag ctgtacccca tcctccctcg caaccgtggt
     3601 cggacgcggc aggctgcctt ttagctggaa ctcatttagc agccgaaccg tgttgtcctt
     3661 gctctcctgc acgttgggct ggtcaaacgg gttgatgccc aggatcgcac ccgcgaccgc
     3721 ggtggcgaac tcccagcgga aaaactccgc ccccaggtcc ttcttgtcgt tcagcttcaa
     3781 ccaaatgacc ggctggcccg catccgccag cgccttcacg catcgctcca ggccggcgtc
     3841 gtgatcgctc gcgaggtaga tatggacgaa gacgcgatcc ttgccataca cggatggatc
     3901 accggcatcc tcgcccgcaa tcggaatcag tcccttgccc tccttgcccg tgctttccgc
     3961 cagtagttgc tccccccaca gaccaaaact gtcgagcgaa ggcgatggca agaaggtgac
     4021 tttgtcatga ccggccttcg ccagcccgcc caggacggtt cccaaccaca cgccggggtt
     4081 ctcgcgcggc acgaaagtgg cgctgcagct agccatcatc gtgcttgccc gctggaggat
     4141 cgcgcgcaca tcaacgccag ccagcgccgc cggcaccagt ccaaacgggg agagcgccga
     4201 gtaacgtcca ccaatttcag ggtcgccgtg gaacacgcga cggaatttcc gctcttcagc
     4261 aagcttgttg agcgatgagc cggcatcggt gatcgccacg aactgttttc cggagtctcc
     4321 ggcgccgctg cgccgaacct tgtgccaaaa ataggcaaag aaggtgttcg gttcggtcgt
     4381 gctaccagac ttgctcgata cgatgaatat cgtcttcgcc aggttgacgg agcgctcgat
     4441 cgccagcacg ttggccgggt cggtgctgtc cagcacgtgc aggttcagga aaccgctccg
     4501 gcgtccgaag gtccgctcca tgaccagcgg cgccaggctg ctgccgccca tgccgagcac
     4561 gaccgcgtct gaatagccct cgccgcgcac ctgctccaca aagctgtcga ggtcgtccat
     4621 cgcctttagg ccatcgtcga ttgcggccag ccagccgagc caaccgctga gcgatttatc
     4681 attcggtttg gcatcaggct tccacagcgt ggcgtctttt ttcatgacac ggtcggcgac
     4741 gtggttcttc gccgcggcat cgagcgcctt gtcaactcgg gcttggaggc cgcccaacga
     4801 cgccgtctgg ttctgcgcga ggttggccct gatggcgccc cgctttcgct cgatctgatc
     4861 cgcgagcgtg ttgaaagact tcgcaaacga tgccacgccc tcgtcttcca actgttgagt
     4921 gaccttcttc atatcgatgc cgactttcgc cagccgctcc atgaagcggt gggcctccgc
     4981 cacatcctcg tcgatggtgt ttcggaccac gccgtgatcg cggaatgcgt cgatcgtggc
     5041 cggtggcatc gtgtccacgg tgtcgggccc gatcagctcc tccacgtaca taacgtcgcg
     5101 gtaggccggg ttcttggtgc tggtgctcgc ccacaacggc cgttgcaccc tggcgccccg
     5161 gtcgcgcaga tccttgaact catcgccgta aaagatctgc ttgaaacgct cataggcgat
     5221 cttcgcattg gcgattcccg ccttgccctt tagcgactcg agttgtttcc gctcttctgg
     5281 actcccggcg gcggcgatct tcgcctccag cagcccatcg cccagcgtgt ccacgcggct
     5341 gacgaagaag ctcgcaacgg acgcgatgcg gtcgatcggt tcgccgcgta ggacacgctg
     5401 acgcagagcg cgaacgaaag cgttggcgac ttctatgtaa cgctcgacag caaagatcag
     5461 cgtgatattg atgttgatgc cgtcggtgag actttgctcg atcgccggca gaccgttctc
     5521 ggtggccggg atcttcacca tcaggttggg ccggtcgaca attccccaga agcgcttgac
     5581 ctccttgatc gttgcggcgg tatcggtcgc tatcaacggg gaaacctcaa gcgagacgaa
     5641 gccgtcggcg ccgttggtgc ggtcatagac tgggcgcagc aggtcgcaag cactccgtac
     5701 atcgtccgtc gccaaccgct catagatccg gttggcgtct gcgcctttag ccacgagcat
     5761 acggatgtcg gcgtcgtagt cgttgccggc cgaaaccgcg tgctcgaaga tcgtcgggtt
     5821 ggcggtgacg ccg
//
LOCUS       DATCPE010000100         2256 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U7219
            k141_320571, whole genome shotgun sequence.
ACCESSION   DATCPE010000100 DATCPE010000000
VERSION     DATCPE010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955033
            Sequence Read Archive: SRR1586279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 16:49:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 640
            CDSs (total)                      :: 631
            Genes (coding)                    :: 627
            CDSs (with protein)               :: 627
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_320571"
                     /isolate="SMAG_U7219"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..154)
                     /locus_tag="VIM45_02900"
     CDS             complement(<1..154)
                     /locus_tag="VIM45_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020267558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY8820267.1"
                     /translation="MAQDSTQNEDPCYVISIAARMVGMHQQTLRYYERVGLIEPSRTR
                     GNIRLYS"
     gene            complement(215..1225)
                     /locus_tag="VIM45_02905"
     CDS             complement(215..1225)
                     /locus_tag="VIM45_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HEY8820268.1"
                     /translation="MPTMNYYDILGVKRDATEKEIRAAYRKLARKFHPDVNPGDKTAE
                     AKFKEINAANEVLSDAEKRRKYDKFGDKWEYSDQIEEQQRQQASAGQWFRTAGRGTRT
                     GGGFGGVETDLGDIFGNVFRGRSKPVARKGENLEHPLDVSLEEAYRGTTRTLTIQNNE
                     PCVGCNGTGLAGEAICRVCDGAGYVTRPRKLEVKIPAGVKIGSRVRIAGEGQPGTSGG
                     SKGDLYLLINVLSSERFERKGDDLYEDVDVPLYDALLGGEAPVLTMTGRVMLKIPAGS
                     QNGKTIRLSGKGMPRLGGSGQGDLYARIRVMLPDHLSQPERELFDQLRALRQPEAAHA
                     SR"
     gene            1490..>2256
                     /locus_tag="VIM45_02910"
     CDS             1490..>2256
                     /locus_tag="VIM45_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY8820269.1"
                     /translation="MVQETTRISEQIREQSRFLTEVRSEVAKVIVGQQYLISRLLIAL
                     LTGNHVLIEGVPGLAKTLSVKTLSDALHLAFVRVQFTPDMLPADLTGTTIYNARDAQF
                     TVRQGPIFANIVLADEINRSPAKVQSALLEAMQERQVSIGGETFPLPAPFMVMATQNP
                     IESEGTYPLPEAQLDRFMIKAVIGYPSRLEERAILDRGISGELGEVRRIVSPEMILAA
                     RAMVREVYLDDRLKDYIIQIISATRNPRAFRMDTLAP"
BASE COUNT          421 a          705 c          633 g          497 t
ORIGIN      
        1 gcgaatacag cctgatgttt ccgcgcgtgc gagacggctc gatcaaccca acgcgctcgt
       61 agtaccgcaa cgtctgctgg tgcatcccca ccattcgcgc cgcgatgcta atgacgtagc
      121 aaggatcctc attctgtgta gaatcctgcg ccatcaatgt ctgcctccta tctgacatct
      181 catccgccca cttggtgcgg cgggtcaggt tggatcatct ggacgcgtgc gcggcttccg
      241 gctgtcgcaa agcgcgaagc tggtcgaaga gttctcgctc cggctgtgat aggtggtccg
      301 gcaacattac ccggatcctg gcgtacaggt ctccttgacc gctgccgccg agtcggggca
      361 tccccttacc ggagagccgg atcgtcttgc cgttctgcga gcccgccggg atcttcaaca
      421 tcacccgtcc ggtcatcgtc agcaccggcg cttcaccgcc gagcaacgcg tcgtagagcg
      481 gcacgtcgac gtcctcatac aggtcatcgc ccttgcgctc aaaacgctca cttgagagca
      541 cgttgatcag tagatacaag tcgcctttgg aaccgccgct ggtgcccggc tgcccttcgc
      601 ccgcgatgcg gacacgcgat ccgatcttga ctccggccgg gatttttact tcgagcttgc
      661 gcggccgggt aacgtagccg gcgccatcac acacgcggca gattgcttcg cccgcgaggc
      721 ccgttccatt acagcccaca cagggctcgt tgttttggat ggtcagagta cgcgtggtgc
      781 cccgataggc ctcttcgagc gaaacgtcga gcgggtgctc gaggttctcg cctttgcggg
      841 caaccggttt gctgcgacca cgaaagacat tgccgaaaat gtcgcccagg tcggtttcga
      901 cgcctccgaa gcctccaccg gtccgtgtcc cccggccagc ggtacggaac cattgtcccg
      961 cgctcgcctg ctggcgctgt tgctcctcta tctggtcgga atattcccac ttgtcgccga
     1021 atttgtcgta tttacgtcgc ttctctgcat ccgacaggac ctcattcgcg gcgttgattt
     1081 ccttgaattt tgcctcggcg gtcttgtccc ctgggttcac gtccggatgg aatttccggg
     1141 ccagcttgcg ataggcagcg cggatctcct tttctgtcgc atcccgcttg acgcccagaa
     1201 tgtcgtaata attcatcgtt ggcatggctg cctcctgctc gaatggccgt cagacgccac
     1261 tccgcggtag ccgcattgag tatcgttgta atgatagtct gataataaca cactaaactc
     1321 tattgacaat aacgccggga gccctatccg tcgtcgacca gcaccacgcg cgccggtgca
     1381 ccgtcgctgc ccaacggctt tagaggtagg cagagcaatt gatatacgct cctaacaccc
     1441 ttgataaccg tttgcggcta tactgtcgca actgagatgg agttgaggca tggtacaaga
     1501 aaccacgcgg attagtgaac aaattcgcga gcagtcccgg ttcctcaccg aagttcggtc
     1561 ggaagtggcg aaggtcatcg ttggccagca gtacctgatc agccgcctgt tgattgcact
     1621 acttaccggg aaccacgtcc tcatcgaggg cgtccccggg ctcgcaaaaa cgctttccgt
     1681 gaagacgctt tctgacgccc tgcaccttgc gttcgtgcgc gtccaattca cgcccgatat
     1741 gttgcccgcg gacctcaccg gtaccacgat ctacaacgcc cgtgacgctc agttcacggt
     1801 gcggcagggg ccgatcttcg cgaacatcgt ccttgcagac gagattaatc gctcacccgc
     1861 caaagtgcag tcggcgctgc tcgaagcgat gcaggagcgc caggtcagta tcggcggcga
     1921 gacctttccg ttgcccgcgc cgttcatggt gatggctacg cagaacccga ttgaaagcga
     1981 aggcacctat cccctcccgg aagcgcagtt ggatcgattc atgatcaagg ctgtgatcgg
     2041 ctatccgagc aggctggagg aacgtgcgat cctcgaccgt ggtatttcgg gggagctggg
     2101 cgaagtgcga cgaatcgtta gtcccgaaat gatccttgcc gctcgggcga tggtgcgaga
     2161 ggtttacctg gatgatcgcc tgaaggacta tatcattcag atcatttcgg cgacccggaa
     2221 cccgcgcgca ttccgcatgg atacgctggc gccctt
//
LOCUS       DATCPE010000101         3106 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U7219
            k141_548943, whole genome shotgun sequence.
ACCESSION   DATCPE010000101 DATCPE010000000
VERSION     DATCPE010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955033
            Sequence Read Archive: SRR1586279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 16:49:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 640
            CDSs (total)                      :: 631
            Genes (coding)                    :: 627
            CDSs (with protein)               :: 627
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3106
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_548943"
                     /isolate="SMAG_U7219"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1229)
                     /gene="dnaB"
                     /locus_tag="VIM45_02915"
     CDS             complement(<1..1229)
                     /gene="dnaB"
                     /locus_tag="VIM45_02915"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEY8820270.1"
                     /translation="MVLEKLPPHDIAAEEAVIASIMVDDEAVFRVSPLLRPEDFFLER
                     NAWIYDTCLALWNRNEPTNPIMIAHELGVRGQLEAIGGPAYLSQLTTELPTAVGVDYY
                     ARIVARDAVYRRMITAAGRMAQMAYEGGADLDTALSRAESLLMALRIGQGTGDFRHIR
                     DLLDKFIQDVGEEDAEGESIAYLRTGFPALDAYLGGMKRSDLLIVAARPGLGKTSLAL
                     TIARNAAVAQNAHVGVFSLEMSGEQLAQRLLSSESGVDQNRLLLMGHLREDEERRVMD
                     SVGKLSALEIYIDDSAGLTIADMRTRARRLAHERGLNLIIVDYLQLMHSGLWGGQENR
                     VQEISFITRSLKGIARELNVPIVACSQLSRAVESRTPHIPMLSDLRESGSIEQDADVV
                     MFIYREDAYMRKDDWQDT"
     gene            complement(1333..1821)
                     /gene="rplI"
                     /locus_tag="VIM45_02920"
     CDS             complement(1333..1821)
                     /gene="rplI"
                     /locus_tag="VIM45_02920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEY8820271.1"
                     /translation="MRNEQKGISEMKVVFLEEAPGARIGDIREVKNGYARNYLLPRGM
                     ALPATITYIEKAQSRMRAEERRQASVDTRARELVEKLGDQPLVMHARVGETGRLYGSI
                     TAADIAEALSVRAGHEVERRTIELGEPIRQVGEHTVHVRLTRNVSTDVTVKVEAQEEE
                     SE"
     gene            complement(1931..2590)
                     /locus_tag="VIM45_02925"
     CDS             complement(1931..2590)
                     /locus_tag="VIM45_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY8820272.1"
                     /translation="MAGEKIIRVMIVDDHAVVREGLRTYLDLSDEIEVVGEARNGREA
                     IERAAVLKPDVVLMDLLMPEMDGIAATRGIKEICPQTNVIVLTSFTDDEHITPALRAG
                     ATGYLLKDISAPDLVKAIEGANKGQAQLHPYVARKLMEQVAMPQRREEPPGAALTPRE
                     LEVLRLIANGMSNKEIARDLVLNERTVKGHVSNILSKLGLADRTQAALFAVRQGIVPM
                     N"
     gene            2831..>3106
                     /locus_tag="VIM45_02930"
     CDS             2831..>3106
                     /locus_tag="VIM45_02930"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HEY8820273.1"
                     /translation="MIDKVFSDPDAAVADIPDGVTIMFGGFASAGSPTSLILALQRQG
                     TRDITGIANNIGLGDKLDVLCERRQLKKMIASFAIRASGGRQSLFEQQ"
BASE COUNT          550 a          998 c          915 g          643 t
ORIGIN      
        1 gtgtcctgcc agtcgtcctt ccgcatgtag gcatcttcgc gatagataaa catgacgacg
       61 tcggcgtcct gttcgatgct tccagattca cgcaggtcgc tcagcatagg gatgtgcggc
      121 gtacgagatt ccaccgctcg cgataactga gagcaggcga cgatcggaac gttgagctcg
      181 cgcgcgatcc cctttagcga gcgggttatg aagctgatct cctgtacccg gttttcttgc
      241 ccgccccaaa gtccggagtg catgagctgc agatagtcaa cgatgatcag gttcagcccg
      301 cgctcgtgcg ccagcctccg ggcccgagtc cgcatgtccg cgatcgtcaa cccggcagag
      361 tcgtcgatgt agatctcgag cgcggatagc ttgccgactg aatccatgac ccggcgctct
      421 tcgtcttcgc gcagatggcc catgagcagc agacggttct gatcgacccc cgattcgctg
      481 gatagcagcc gttgtgcaag ctgttcaccc gacatctcga gcgagaagac accgacgtgg
      541 gcgttctgcg ccaccgcggc attgcgggcg atcgtcagcg ctagactcgt ttttccaagt
      601 cccggcctcg cggcgacgat taagagatcg gagcgcttca tcccgcccag gtacgcgtcg
      661 agcgccggaa aacccgtccg aaggtacgcg atcgactcgc cttcggcgtc ctcttcgccg
      721 acatcctgga tgaacttatc gagcaggtcg cggatgtgtc gaaagtcccc cgtgccctgc
      781 ccgatgcgca gcgccatcag caggctctcg gcgcggctca gggccgtatc caggtcggcg
      841 ccgccctcgt aggccatctg cgccatacgt cctgcggcgg tgatcatgcg gcgatagact
      901 gcgtcgcgcg ctacgatgcg ggcgtagtaa tcgacgccga ccgcggtagg aagttcagtc
      961 gtaagctgcg ataggtaggc cgggccgccg atcgcttcga gctggccgcg gacgcccagt
     1021 tcgtgggcga tcatgatcgg gttagtcggc tcgttccggt tccagagcgc caggcaagtg
     1081 tcgtagatcc aggcgttacg ctcgagaaag aaatcctcgg ggcgcagcaa gggcgagacc
     1141 ctgaaaactg cctcgtcatc caccatgatc gaggcgatca cggcctcttc ggcggcgatg
     1201 tcgtgtggtg gcaacttctc gagaaccacg gtgtgcgcct cacgacatgg ctgaaccgaa
     1261 gctccgtaat tctataacaa accccgggag ccagccacgc cgaacccccg gggttatgac
     1321 aagccgcgcc ggttattcgg attcttcttc ctgggcctca accttcacag taacgtcggt
     1381 gctcacgttg cgagtcaagc gcacgtgcac cgtgtgttcg cccacctggc ggatcggctc
     1441 accgagctcg atcgtgcgcc gttcgacctc gtggccggct cgcacggaca acgcctcggc
     1501 aatgtccgcg gcggtaatgg agccatagag ccgccctgtc tcgccgaccc tggcatgcat
     1561 taccagcggc tggtcgccga gcttctcgac gagttcgcgc gcccgggtat cgaccgacgc
     1621 ctggcgccgc tcttcggcac gcatcctgga ctgggctttc tcgatgtagg ttatagttgc
     1681 aggcaacgcc attccgcgcg gcagcaggta attgcgcgcg tagccgttct ttacttctct
     1741 tatgtcgcca atacgcgccc ccggcgcttc ttcaaggaaa actactttca tctcgctgat
     1801 ccctttttgt tcgttgcgca cgtatgaaac tgcgccacga tggatttccg ccggcgcaca
     1861 ccgattacac tataagttgg gccagcggcc cgctcaagga gaggcgacgc cggcagggag
     1921 gttctagctg tcagttcatc ggaacgatgc cctggcgtac ggcaaataac gcagcctggg
     1981 tacgatctgc cagacccagc ttgctgagga tgttgctgac gtggcccttg accgtccgct
     2041 cattcaaaac gaggtcgcgg gctatctctt tgttgctcat gccgttggcg atcaggcgaa
     2101 gtacctcgag ttcacggggc gtcaatgctg cccccggcgg ctcctcgcgc cgctggggca
     2161 tcgccacctg ctccatgagc ttgcgcgcca cgtaggggtg cagttgcgcc tgccctttat
     2221 ttgcaccctc gatcgccttc accaggtcgg gtgcggagat gtccttgagg agataaccgg
     2281 tggcgccggc acgtaaagcg ggggtgatgt gctcgtcgtc ggtaaagctg gtaagcacga
     2341 tcacgttcgt ctgcgggcat atctctttta tgccgcgcgt agcagcgatg ccgtccattt
     2401 cgggcatcag caggtccatg agcacgacgt cgggcttcaa taccgcggcg cgctcgatcg
     2461 cctcgcggcc gtttcgcgcc tcgccgacca cctcgatctc atcgctcagg tcgagatatg
     2521 tccgtaggcc ctcgcgcacg accgcgtggt catccacgat catgacgcga atgatcttct
     2581 ccccggccat caagtcgtcc ccatctcctc cacaacctgt ccgttttcgc tcgtcctcgc
     2641 tcatcccggt aaccaatcgg gtatcctcca gtgccggttg cttcacctgt tctctcaaat
     2701 catgtaccgc tgctgcaagc cgcaagcgct tgcagaggag cgcgcccacg tcggaacatg
     2761 cggtatgttc cggagctacc gaaaacgctc cgtgtcggtt cggttcagtt ggtagagagg
     2821 tcagtccggc ttgatcgaca aagtcttctc cgatcccgac gccgcggtgg ccgatatccc
     2881 tgacggcgtg acgattatgt tcggcggctt cgcgtccgcc gggtcaccca cctcgctgat
     2941 cctcgcactc cagcgccaag ggactcggga tatcaccggc atcgccaata acatcgggct
     3001 gggcgacaaa ctcgacgtcc tgtgcgaacg gcgccagttg aagaaaatga tcgcgtcgtt
     3061 cgccatccga gcctcaggtg gacgccagag tctctttgag cagcag
//
LOCUS       DATCPE010000102         7300 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U7219
            k141_289969, whole genome shotgun sequence.
ACCESSION   DATCPE010000102 DATCPE010000000
VERSION     DATCPE010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955033
            Sequence Read Archive: SRR1586279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 7300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 16:49:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 640
            CDSs (total)                      :: 631
            Genes (coding)                    :: 627
            CDSs (with protein)               :: 627
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7300
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289969"
                     /isolate="SMAG_U7219"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(93..1541)
                     /locus_tag="VIM45_02935"
     CDS             complement(93..1541)
                     /locus_tag="VIM45_02935"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEY8820274.1"
                     /translation="MVRTLKALGYFREDGGRSSKPGRAPADAVDSLADQNRAFLEFCK
                     QEGHEVAATFLDTAAEKDDFSGFRQLLEYLKRQRGAPCLVVVTSVDRLGRDAMQATRC
                     LYQIDGLGGKLVTLDGKPDPAASLLEHWVKTERSPDVGDRVREAMRRKAIRGEVLGRP
                     PYGYQVGPKHRLEPVPDEAPVIRYIFRLYTREGLGIRLIARRLNEEGFRTRRDGNWSM
                     VTIRDILLNRAYLGTYTRFGVRVPGSHTALISPEDFRRVQDRMASRRTGGGERRVRQY
                     LLSGLAYCGYCGNKMIGVSRRQTWKRRGDGSEGEAEYRYYQCLSRTNQSMCDYHTWRA
                     GILEEEVRRQAAAAIVEALAGADSAEIAGPTVEVEVEKLRVRLRKLDRTLEQHMHAAA
                     AQEMSMERLRELSVELTQQQMETDDALRQLEGRRKIVESFGDQRRARATVLEALTTGW
                     QAADFAASHALIRQTVARVTVLDDGALVRTHV"
     gene            complement(1619..2320)
                     /locus_tag="VIM45_02940"
     CDS             complement(1619..2320)
                     /locus_tag="VIM45_02940"
                     /inference="COORDINATES: protein motif:HMM:NF014630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HEY8820275.1"
                     /translation="MVHISDLYVLQEIDSQIDALERTLVDLRSRQGNDDSVAGAKERL
                     IALDAAAAEAAREQRAADEEVADGRPKLSAVEEKLYSGTVTNSKELRDLQRELEALQR
                     QQAQREEAALAAMTHAEESRVEAQAATQSLHDGEAAWGEEQQQIAAQLEQIEVDREHL
                     RNRRQVATQPVDAQTMALYERLRRMRRGEAVARVQRGACLGCRIMLPSQIFQKARSGM
                     AVVQCSSCERILYVG"
     gene            2613..3131
                     /locus_tag="VIM45_02945"
     CDS             2613..3131
                     /locus_tag="VIM45_02945"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEY8820276.1"
                     /translation="MREPIGSRRRYQADEEFVPVEGERLRVRVQGSVDMLRTDRGILA
                     SAHVTSSAEGECDRCLLSVTYPVTMDVEEEFLPTVDPITGGKLPPPVEPEASTINSHH
                     ILDLTDVVRQSWLLVSPMQVLCRDDCAGLCPECGADKNNRPCACSEAPVDSRWAALSA
                     LRNVNGDHHEEN"
     gene            3134..3331
                     /gene="rpmF"
                     /locus_tag="VIM45_02950"
     CDS             3134..3331
                     /gene="rpmF"
                     /locus_tag="VIM45_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEY8820277.1"
                     /translation="MAPLPKKKYASSRQGKRRSHIHLATVTAVACPQCHAPRLSHHAC
                     PTCGYYKGREAVAIEKPALNG"
     gene            3346..4332
                     /gene="fabD"
                     /locus_tag="VIM45_02955"
     CDS             3346..4332
                     /gene="fabD"
                     /locus_tag="VIM45_02955"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEY8820278.1"
                     /translation="MALTVPTGHASIPPDADSRPNLAWVFPGQGSQVVGMGRDLYERF
                     DAARDIFDAADRALGFSLSRLCFEGPEEELLNTVNTQPAIVTTSIACFAAAYGRLDIV
                     DGAPAYVAGHSLGEYSALIAAGVIPFPEGVRLVRERGRLMQEAGERNPGTLAAIMGLD
                     DSIVEEVCQETGAEICNINGAGQIVIGGPRDAVLLAMDLAKARGAARVIQPNVSGAFH
                     SSLMRPAADGMRRALLDANLLDPRIPIIGNCDGVPLESARAIKDELARQVATAVQWQR
                     SVEYMVRAGVSTFVEIGPGRVLSSLIKRIARGVSVRAVGDAAAIEGDAAGSA"
     gene            4329..5102
                     /gene="fabG"
                     /locus_tag="VIM45_02960"
     CDS             4329..5102
                     /gene="fabG"
                     /locus_tag="VIM45_02960"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011130701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEY8820279.1"
                     /translation="MTSGPFPGTRVLDGKTALVTGASGGIGKAIAVALAASGASIVVN
                     YHSNRQAAEETLAAIAANGSEAIAVEADIADPASVDRLFQESAKRFSSPDILVNNAGM
                     TRDGLLMRMSPEDWDRVIAVDLRGAFLCTRAALRGMLRRRWGRIVNISSVVGLVGNAG
                     QANYAAAKAGLIGLTMATAREVASRGITVNALAPGFIETNMTAVLTPTQRDAALRRVP
                     FERFGVPEDVAPAVVFFASPGASYITGQVLAVDGGMVMG"
     gene            5099..5605
                     /gene="nusB"
                     /locus_tag="VIM45_02965"
     CDS             5099..5605
                     /gene="nusB"
                     /locus_tag="VIM45_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEY8820280.1"
                     /translation="MTGNPDSQSNAERESGEDQKAPGMSRQQRKDRLPAQRHRARTLT
                     LQSLYEADQSGHDPVNSIERLIIDEQAGNEAAEFARGLVAGIKRSRAEIDNYISTSAP
                     QWPVEQLAVVDRNILRIAIYEMLIENETPMRVAVNEAVELAKAFGSDASPRFVNGVLG
                     SLSAHKGG"
     gene            5694..5954
                     /gene="acpP"
                     /locus_tag="VIM45_02970"
     CDS             5694..5954
                     /gene="acpP"
                     /locus_tag="VIM45_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011309637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEY8820281.1"
                     /translation="MPTVFERVRKIIVDQLGVDEEDVVPAASFVDDLNADSLDLVELI
                     MSLEEEFSTGVQTVEISDEDAEKIATVQDAVDYIKDRGIEDS"
     gene            6068..6568
                     /locus_tag="VIM45_02975"
     CDS             6068..6568
                     /locus_tag="VIM45_02975"
                     /inference="COORDINATES: protein motif:HMM:NF014470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEY8820282.1"
                     /translation="MDFLGIGVGLPQLLLVLLVAMLVLGPERLPEVARQLAKGIRTLR
                     SYATEVQGQFEGELGDLRSEFLDIQRDLTSIHGDLRGGLMELDSSVRSVQTEMNSAFS
                     TANTPLAPAGTPEIDEPAPPVSLDYVPAAPRRYSSTTAATNDTSTGVTEPAADGRLPD
                     FRPRSR"
     gene            6615..>7300
                     /gene="tatC"
                     /locus_tag="VIM45_02980"
     CDS             6615..>7300
                     /gene="tatC"
                     /locus_tag="VIM45_02980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEY8820283.1"
                     /translation="MATLQQDFEEPEGREEVDEGGQELTIMQHLYELRRRLFAMALAI
                     AIGTGIGLFLAKRGIDFLERPAKSVYPNFAPSQIEPLEFVGAYFKVALLIGLIIAMPI
                     LVYEILAFVVPALTRKERRWMFPILFGVFGLFLSGVVFSYYLVVPRALDFILNFGKGQ
                     VDIHIRVGLYVDFVVRLVFWTGVLFELPIVMMVPAKFGIIRARKYLKWWRYAIVLGFL
                     AAAAVIPNISP"
BASE COUNT         1380 a         2215 c         2235 g         1470 t
ORIGIN      
        1 ctaaggaggg tttcaggtac agggcacccc caacaccccg cttagcacga ccacatatat
       61 gatagcgact gcgcaggcgg gggacaacgc gattagacgt gagtgcgtac cagcgccccg
      121 tcgtcgagca ccgtaacgcg cgccactgtt tgccgtatga gcgcatggct cgctgcgaaa
      181 tcggccgcct gccagccggt cgtcaacgct tccagtaccg tcgcccgcgc tcgacgctga
      241 tcgccaaacg actcgacgat tttgcgccgc ccttcgagct gcctaagcgc atcgtcggtt
      301 tccatctgct gttgcgtcaa ctcgacgctt aactcgcgca gccgctccat cgacatctct
      361 tgcgcggcgg ccgcgtgcat atgctgctcc aacgtccggt caagtttccg caatcgaact
      421 cgcagctttt cgacctcaac ttctactgtg ggccccgcga tttcggcgga gtcggcgccg
      481 gcgagcgcct caacgatcgc cgccgcggcc tggcgcctga cttcttcttc caagatgccg
      541 gctcgccacg tgtgatagtc gcacatgctc tggttggtgc ggctcaggca ctggtaatag
      601 cggtattcgg cttcgccttc gctgccgtcg ccacgacgct tccacgtctg ccggcggctg
      661 acgccgatca tcttgttgcc acagtagccg caataggcaa gcccggacag aagatactgt
      721 cgaacgcgcc gctctccgcc accggtgcgc ctgctggcca tgcggtcttg tacgcggcga
      781 aagtcttccg gcgaaatcag tgccgtgtgg ctgcccggaa cgcgaacgcc aaagcgggtg
      841 taggtgccaa gatatgcccg attcaggaga atatcgcgga tggttaccat gctccagtta
      901 ccatcgcgac gcgtgcgaaa gccctcctcg ttgagacggc gggcgatcag gcggatgccc
      961 agcccctcac gcgtatacag cctaaaaatg tagcgaatta ctggtgcttc atcgggcacc
     1021 ggctcaaggc gatgcttggg gccgacctgg tagccatatg gcggccggcc aagcacctcg
     1081 ccccgtatcg cctttctccg catcgcttcg cgcactcggt cgccgacgtc aggtgagcgt
     1141 tcggtcttga cccaatgttc cagcagagac gctgccgggt caggtttgcc gtcaagcgtt
     1201 acaagcttgc cgcccaaacc atcaatctga tagagacagc gcgtagcctg catggcatcg
     1261 cgtcccagac ggtcaaccga ggtgacgact acgaggcagg gcgcgccgcg ttggcgtttt
     1321 agatattcca ggagttgccg aaatccggaa aagtcgtctt tctcggcggc ggtatcgaga
     1381 aacgtggcgg cgacctcgtg gccttcctgc ttacaaaatt cgagaaaagc gcgattctgg
     1441 tccgccagcg agtcaacagc gtcggcaggg gcgcgcccgg gtttcgacga ccggccgcca
     1501 tcttcccgga agtatcccaa cgccttcaat gtccgaacca ctcgatcttt cccgcttgac
     1561 catcgtttgc cggcgccatc ggcatccggc tgcgaatggt atcttcgtgt atccctggtt
     1621 atccgacgta caagatgcgc tcgcagctgc tgcactggac aacggccatc ccgcttcgcg
     1681 ccttctggaa tatctgcgat ggcagcataa tgcggcagcc gaggcaggcg ccgcgctgca
     1741 cgcgggcaac ggcttctccc cggcgcatgc ggcgtaaccg ttcgtaaagg gccatggttt
     1801 gcgcatcgac cggttgcgtc gcaacttgtc tgcggttccg caagtgctcg cggtcaactt
     1861 ctatctgttc gagttgagcc gcaatttgct gctgttcctc tccccatgcg gcctcaccgt
     1921 cgtggagcga ttgcgtggcc gcctgcgcct ctacgcgcga ttcctcggcg tgagtcatgg
     1981 cagccagtgc cgcctcctct cgctgcgcct gctgacgttg caaagcctcc agctcccttt
     2041 gaaggtctcg cagctccttg gagttggtca cggttccgct gtacagcttt tcctcgacag
     2101 cgcttagttt cggccgtccg tcggcgacct cctcatcggc agcgcgctgc tcgcgtgcag
     2161 cctcggccgc cgccgcgtca agcgcgatta gcctctcttt tgctcccgcc acgctatcgt
     2221 cattcccctg ccgtgagcgc agatcgacca gagtacgctc cagcgcgtca atctgggaat
     2281 cgatctcctg gagcacatac agatccgaaa tatggaccat ttattcctct gttgagcaat
     2341 gagcaatgag cgatgagcga aaggctgtgc gcagcctgaa cctgcgtgaa gcggggacgt
     2401 ggtacgagcc ggattatatg cccgtgggac cggccgggac aaggcaaaac tgggaatttt
     2461 cgcagcttcg gtatagtagt tcgcagaatt cgttaagggg gcttcggccg accgcataga
     2521 tcgttacgtt gacagcgccg gataatcgta agctatactt gagtctgtcc cgcaaagggt
     2581 tacccgtcgt gcagttcaac gtttcactcc tgatgcgcga gcctataggc tcgcgcaggc
     2641 gttatcaagc ggatgaggag tttgttccgg tcgagggcga gcgacttcgg gtacgtgttc
     2701 aaggttccgt tgacatgctg cgcaccgacc gcggaattct cgcctctgct cacgtgactt
     2761 caagcgccga aggcgaatgc gaccggtgcc tgctatcggt gacgtacccg gtaacgatgg
     2821 acgtcgaaga ggagtttctt ccaaccgtcg atccgattac gggtggaaaa ctccctccgc
     2881 ctgtggagcc cgaagcatcg acaataaatt cgcaccatat cttggacctg acggatgtcg
     2941 tccgtcagtc ttggctgctt gtgtcaccga tgcaagtgct ttgccgagac gattgcgccg
     3001 gcctctgccc ggagtgcggc gctgataaga acaaccggcc gtgcgcctgc agcgaggcgc
     3061 cggtcgatag tcgctgggcg gctctctctg cgctgcgaaa cgtgaacggc gaccatcacg
     3121 aggagaacta aaagtggcac cgctcccaaa gaagaaatac gcgagttccc gccagggcaa
     3181 gcggcggagc cacatccact tggccacggt tacggcggtg gcgtgtcctc aatgtcatgc
     3241 tccccgccta tcgcaccacg cctgcccaac ttgtggctac tacaagggcc gcgaagcggt
     3301 ggcaatcgaa aagccggcct tgaacgggtg agcaggaggc gcaacatggc gctgacggtc
     3361 cccaccggac acgcatctat tccgccagat gctgactcac gccccaatct cgcgtgggta
     3421 tttccgggtc agggctccca ggtcgttggc atgggacgcg acctgtacga gcgattcgac
     3481 gccgcgcgcg acatattcga cgcagccgac cgagcgctcg gtttctcgct gagcaggctc
     3541 tgctttgagg ggcccgagga agaactgctg aatacggtca acacacaacc cgcgattgtg
     3601 acgaccagta ttgcgtgctt cgcggccgcc tacggccgtc tcgacatcgt cgacggcgct
     3661 ccggcctatg tagctggcca tagcctgggt gagtactcgg cgctgatcgc ggcaggcgtg
     3721 attccttttc ccgaaggcgt ccgcctggta cgcgaacgcg gccggctgat gcaagaagcc
     3781 ggcgagcgca acccggggac gctcgcagcg attatgggcc tcgatgattc gatcgtcgaa
     3841 gaggtgtgcc aggagaccgg ggccgagatc tgcaacatca acggcgccgg ccagatcgtc
     3901 atcggcggtc cgcgcgatgc ggtactcctg gcaatggacc ttgcgaaggc gcgtggcgcc
     3961 gctcgcgtta tccaaccgaa cgtcagtggc gcctttcatt cgtcgctaat gcgcccggcc
     4021 gccgatggca tgcgccgcgc cctcctcgat gcaaacttgc tcgacccacg catccccatc
     4081 atcggtaact gcgacggcgt gcccctcgaa tccgcgaggg cgattaaaga cgaattggcg
     4141 cggcaggtgg caacagcggt gcagtggcaa cgctcggtgg agtatatggt ccgggcaggc
     4201 gtctctacgt tcgtcgagat cggtccgggt cgcgttctca gtagtctgat taaacggatt
     4261 gcgcgcggtg tctccgtccg cgccgtcggc gatgcggccg ccatcgaagg cgatgccgcg
     4321 ggcagcgcat gacatcgggg cccttccctg gaacccgcgt actcgatggc aagactgcac
     4381 tcgtcacggg cgccagcgga gggatcggca aagcgattgc ggtggctctc gcggcctccg
     4441 gcgcttcgat tgtggtcaat taccattcaa acagacaggc agcggaagag acgctcgccg
     4501 caatcgcggc aaacggttcg gaagcgatcg ctgtcgaggc ggatatcgcc gatccagcgt
     4561 ccgtcgatcg gctgtttcaa gagagcgcaa agcgtttcag tagcccggat atcctggtaa
     4621 acaatgccgg catgacccgg gatggtctgc tcatgcggat gagtcccgag gactgggacc
     4681 gcgttatcgc ggtcgacttg cgtggggcat ttctgtgtac acgcgccgcc ttgcggggca
     4741 tgttacgccg ccgttggggg cggatcgtca acatcagcag cgtcgtcggt ctcgtgggaa
     4801 acgccggcca ggccaactac gcggccgcca aagccggatt gatcggcctg acaatggcga
     4861 cagcgcgcga agtcgcttcg cgcggcatta ccgtgaacgc gctcgcgccg ggctttatcg
     4921 aaacgaacat gacggccgtg ctcacgccga cacagaggga cgcggcccta cgacgggttc
     4981 cgtttgagcg cttcggtgtt cccgaggacg tggcccccgc cgtagtattc tttgcttcgc
     5041 ccggtgcatc atatattacc ggacaggttc tcgcggtcga tggcggcatg gtgatgggat
     5101 gaccggtaat cctgattcgc agtcgaatgc agagcgcgaa tctggggaag atcagaaggc
     5161 gcctgggatg tcgcggcagc agcgaaaaga ccgcctgccg gcacagcgtc accgagcgcg
     5221 gacgctgact ctacagtccc tttacgaagc cgaccaatcc gggcacgacc cggtgaattc
     5281 gattgaacgg ctgataatcg acgagcaggc ggggaatgaa gccgccgaat tcgcacgcgg
     5341 attggtggcc gggattaagc gaagtcgcgc tgaaatcgac aactacatca gcacatccgc
     5401 accgcagtgg ccggtcgaac agcttgcggt ggtggaccgc aacatcctgc gcattgcaat
     5461 atatgagatg ctgattgaga atgagacgcc catgcgagtg gccgtgaatg aggccgttga
     5521 gcttgcgaag gcgtttggtt cagatgcgtc tccgcgcttt gtcaatgggg ttctgggttc
     5581 cttgagcgcc cacaagggcg ggtaaaattg cccgccgcgc gtaccacgcg acccccaagc
     5641 cgatctacga tgcagcgcat aaccacaaga aaataaagga acggggtgag ccggtgccaa
     5701 cagtcttcga acgggtacgc aagatcatcg tcgaccaact tggcgtcgat gaggaggacg
     5761 tcgtgccggc cgcatcgttc gtggatgacc tcaacgccga ctcgctcgat ttggtcgagc
     5821 tgattatgtc gctggaagag gaattctcca cgggcgttca gaccgtggag atctccgacg
     5881 aggacgccga gaagatcgcc acggtgcagg atgcggtgga ctacatcaag gaccgcggca
     5941 tcgaagatag ttagagcacg ccgcgcaatg cgtctcgggg gcgcgcattc gcgtcgtcgt
     6001 tcggtgcgtc acgctgtaga cttatatcgg cagcagtggt gaatcgaacg tagcggcgga
     6061 ttcctctatg gacttcctcg ggatcggcgt tggcttaccc caacttctgc tcgtcctcct
     6121 ggtggcgatg ctggtgctcg gacccgagcg cttgccggaa gttgcccgtc aactggcaaa
     6181 aggcatccgc acgctgcgca gctatgcaac tgaagttcag ggacagttcg agggcgagct
     6241 cggggacctg cgtagtgagt ttctcgatat ccagcgcgac ctgacgtcaa tccatggcga
     6301 cttgcgcggc ggcctcatgg agcttgattc gagcgttcga tcggtgcaaa ccgagatgaa
     6361 ctcggcattt tccactgcta acactccttt ggcgccagcc ggaacaccgg agatcgatga
     6421 gcccgcgccg ccggtgtcac tcgattacgt cccagcggcg ccgaggcggt actcatccac
     6481 cacagcggcg accaacgata ccagcaccgg cgtcactgag ccggctgcag atggacgctt
     6541 gccggacttt aggccgagga gccgctagca gcgaattgcc ggcgggatga caaggcccgt
     6601 ccagggagcc cggagtggcg acgcttcaac aagacttcga agagccggag ggccgagagg
     6661 aggtcgatga gggtgggcaa gagctcacca tcatgcagca cctgtacgag ctccgtcgcc
     6721 gactttttgc gatggcgctt gcaattgcca ttggcaccgg tatcggcctg ttcctggcga
     6781 aacgtgggat cgattttctc gagcgcccgg ctaagagcgt ctacccaaac tttgcgccat
     6841 ctcagataga gccgctggaa ttcgtcgggg cgtacttcaa agtagcctta ctcatcggcc
     6901 tgatcatcgc catgcccatc ctcgtctacg agatcctggc attcgtggtg ccggcgttga
     6961 cgcggaaaga gcgccgctgg atgttcccaa ttttgttcgg cgtattcggg ctgttcttgt
     7021 cgggtgtcgt gttctcatat tacctggttg tgccgcgggc tctggacttc atcctcaatt
     7081 tcggaaaagg ccaagtcgat attcacatcc gtgttggatt gtacgtggat ttcgtcgtgc
     7141 ggctcgtgtt ctggacgggc gtactatttg aattgccgat cgtgatgatg gtgccggcaa
     7201 aattcggcat tatccgcgcc agaaaatacc tgaaatggtg gcgatacgcg attgtccttg
     7261 gtttcctggc tgctgcggcc gttatcccga acattagtcc
//
LOCUS       DATCPE010000103        31625 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U7219
            k141_549222, whole genome shotgun sequence.
ACCESSION   DATCPE010000103 DATCPE010000000
VERSION     DATCPE010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955033
            Sequence Read Archive: SRR1586279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 31625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 16:49:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 640
            CDSs (total)                      :: 631
            Genes (coding)                    :: 627
            CDSs (with protein)               :: 627
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31625
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_549222"
                     /isolate="SMAG_U7219"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            180..401
                     /locus_tag="VIM45_02985"
     CDS             180..401
                     /locus_tag="VIM45_02985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8820284.1"
                     /translation="MAGRVDGEVALIMGAGQTHVQVCPKVHGHAYRSDDGELVNGKRL
                     EWNGPTRRRRAMSVLQQAGIWAASGPEVV"
     gene            complement(456..1862)
                     /locus_tag="VIM45_02990"
     CDS             complement(456..1862)
                     /locus_tag="VIM45_02990"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY8820285.1"
                     /translation="MNRQLKHESSPSDAGPIDENISTRRGGLKTSDRGGRTSSERSGA
                     VSITGAESRGPLEGLRAVVFTQAWAGTFATQMLSLMGAEVIQIEVRSRPDNWRGGYSA
                     VVPPALRSIPTAMHGWNCNPNYNSVNLSKEAITLDVASPQGARMIRQLIVTADIVAEN
                     FAPRVMGNLGLDYSSLVEIRPDIIMLSLSAYGATGPYSNFPGIGGTVEPMAGMSALMG
                     YEDGPPINSGNMVPDAVAGSYGAAALLIALHYRERTGRGQYIDLSMQEANMCFIADAL
                     MDYSANGRVRGRMGNHHPTIAPHNLYPCTGSDRWIALAASTDAEFSRLCTAAGRPDWL
                     LDKRFIGAADRKRNETALDEQIGAWTSTRDAFELEAVLHEAGITAGAVRNADDVLVFE
                     QLRVRGMLQPIEHPEAGIHDQVTVPWRLSRTPGTRVRYSPLFGEHSRDVFRRLLATTD
                     EEYDQLDALGITGSTEPV"
     gene            complement(1859..3043)
                     /locus_tag="VIM45_02995"
     CDS             complement(1859..3043)
                     /locus_tag="VIM45_02995"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY8820286.1"
                     /translation="MAALDDLRVLDLSGHVAGPYCAKMLADFGADVIKIEPPGGESGR
                     RLPPFIDDVPGPDRSAFFLFLNTNKRSVTLDVTTPSGMAILRQLIEDTDIIVESFPPG
                     MLDELGLGYESMEGIRPGIILTSVTPFGQTGPWRDLPINDLVAYACSGWASINGWPNR
                     EPLKASGYQGSMQAGLLAFVATMNAVVYRDRNTGKGQHVDVSILEPLLATFAPIVLQA
                     QYEGAPHGRRAPNFQNGPVPAKDGYFALTLSRAHFWRDAMNELGLPDLAYDQRFYESK
                     SRREHAPLVAPLVESRIAGREKQELFEALGTLRVVGGMVLTTEEIFADRHVRERRFLV
                     ESDDVDGRRISYPGAPFKMSETPWHLSKTAPRLGQHTAEVLGAIGLQPSELSSLATAA
                     VI"
     gene            complement(3084..3878)
                     /locus_tag="VIM45_03000"
     CDS             complement(3084..3878)
                     /locus_tag="VIM45_03000"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEY8820287.1"
                     /translation="MTAVSNVIECPVDQNGVGWLWMNRPDRLNALNFDLITQASEQLK
                     TWERDPNVRCIVISGRGRAFCAGDDVKGMSDGSPEWDTMEYTLRREAGWERLFKTIYD
                     LRKPVIAAINGNALGAGAFIALAADIRIMKEDAPFGFVFVKIGITGGNALVTRYVGLT
                     RASEMVLTGETVNGTEAERIGLVNRAVPAEAFDAEVEAWARKLAAGPTRVLGIAKHTL
                     HRGLYSDNETAFAINALSSKLSQETKDSTEGKLALRERRPAAFTGQ"
     gene            complement(3875..4801)
                     /locus_tag="VIM45_03005"
     CDS             complement(3875..4801)
                     /locus_tag="VIM45_03005"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8820288.1"
                     /translation="MRNPDFFRPGLPYLDAVEIKVINDASARLANFLSGAVAYHELAG
                     PQDVDQVKKTGAQITKYGSFSASHKVFNVAPNGQKEFRDVRVRQAFDLAIDRDVILQN
                     VVQGDGFWGGPCIPPGYGDMALSQDEVKGLYKQDLKKAKQLMTAAGVDSLAVPMEYSN
                     IDNLAGDEMPLMKQMLAQIGINIDLKPAERTAYLQHQVDGNFKIQTIGSGAGPDPDSL
                     LFPYYHSKGGKNWGRASDPELDARIEKERGMLDVKERNAYIKQMTKDWQNYLYRLHTV
                     YQNLYVAWAGPIKGTFVPKGADWQGMEGVSLG"
     gene            complement(4974..6275)
                     /locus_tag="VIM45_03010"
     CDS             complement(4974..6275)
                     /locus_tag="VIM45_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HEY8820289.1"
                     /translation="MKKTYRPYQPDQPLLLPASLQDYLPEGDLAYFVPEVVAGLDLSA
                     IFAPYEREMRGYPPYHPQMLVTLLLYAYARGVYSSRRIAFACSHDLGFMVVTARQTPD
                     FRTIAAFRTRHLDALGALFGQVLQLAAEAGLVKLGHVALDGTKVKANASRHKAMSYGR
                     MEERASALEAEVAGWLAQAEETDAAEDAVLGDRQGDELPAHLATKEKRAVAIRKAKAA
                     LEERAREKAKAEGSDPEQAVVAAKTQYNFTDPESRIMKTPDGFQQCYNGQAAVDADSM
                     VIVGQELTAAPTDVQRLAPMLETVKEETGRYPEQVSADAGYASEANLQHCADLGVDAY
                     VALGRLKHDAPPEQVAEARPSGNRWPARARMREKLGTAAGRAAYANRKQTVEPVFGHI
                     KACRGFRQFLLRGLTATQGEWALLCTVHNLLRIYRCGAAIT"
     gene            complement(6193..7791)
                     /locus_tag="VIM45_03015"
     CDS             complement(6193..7791)
                     /locus_tag="VIM45_03015"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8820290.1"
                     /translation="MGSGIDRSLRGAMMTYLWRMRPYFRQVAGELVLGSVCGIVMNTA
                     VVGPAIFLGRAIDKALALERGEATGWEVTVAALLLIAGTLLTEIPRIGKRWWLMTANG
                     RIRCNIRADALRGTLSRPLADLHRTSIGELMARIVGDVEVLGIGVREFTIETWDTVLF
                     SLSFVVAMLVINPGLTAIALAPVPVAMLIAHSSGRWVSARTTHAREANADLTASIQEL
                     LAGFRVLRFFGRGQAATAEVAALSESYAARNLSTARLRLGLQPAYSTLMMAGVLFVVW
                     QGGERVVGGAMTLGTFVGYLNLFIRFVDRGYRMPQLVNSIQSGAAAYARLEPLLAPPL
                     PVEGEPPLASFNPERVAGIDREDEQPGRRDSGPVGVSLRGLSFRYPGASDQALSDLSL
                     EIPAGSFVAVTGPIGSGKSALARALLGVYPIESGDLVLSGEAGTPLDISHRAGTIGYL
                     PQDAHLFSGSVRENVLLGTAEAPPEFVNHVVRLAALGACPSNRDRQDARRLGSCGYEE
                     DISAVPARPTAAAARFPAGLSAGG"
     gene            7956..8429
                     /locus_tag="VIM45_03020"
     CDS             7956..8429
                     /locus_tag="VIM45_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014691237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Chromate resistance protein ChrB"
                     /protein_id="HEY8820291.1"
                     /translation="MDANRQWVLLAYRLPREPSTPRITLWRKLRRFGAVQVLDGLAGL
                     PLTPRTREQFEWLADEVIEADGEASVWMAAPLSAAEERDLTSRLTRAIVDEYSAITTA
                     AQAALREPSLARRRMLARLRRELRRIDERDYVLPSARNDAHAVVAALTATLEVPA"
     gene            8426..8857
                     /locus_tag="VIM45_03025"
     CDS             8426..8857
                     /locus_tag="VIM45_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014444802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate resistance protein ChrB
                     domain-containing protein"
                     /protein_id="HEY8820292.1"
                     /translation="MKWATRPGCHIDRAACAWLIQRYIDPAAAFVFVEDTAGVPPDAT
                     PFDMRGVDLSHHAGRCSFETFLVRYRLDDPVLWDIAHIVHEADLADERYDAPEAPGLD
                     VLLQGLVLVRGDADVLAIAMTLFDGLYAIRKQLALATSFPR"
     gene            8878..9240
                     /locus_tag="VIM45_03030"
     CDS             8878..9240
                     /locus_tag="VIM45_03030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8820293.1"
                     /translation="MFAVSDILVDALIAGVLAGALLFVWPWTRRRGCFAVAAISTFAG
                     FTAWNLVLNSTHGSNFNVDAPVIGLSWADAGSGVLAFVATALVLGLALERTEPAGRVI
                     GAAAIAGVVAMGVDLFVL"
     gene            9263..9712
                     /locus_tag="VIM45_03035"
     CDS             9263..9712
                     /locus_tag="VIM45_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007835563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate resistance protein ChrB
                     domain-containing protein"
                     /protein_id="HEY8820294.1"
                     /translation="MKWVTRARPMVDRIACPWLIKRFVDKDAEFLYVPGDQVLAVAER
                     EGATPYDIPNAELGHHGSECSFDAIIKKYELTDPALQQLALIVRGADTDARNLTPESR
                     GLVAIATGFRLAYQNDHEQLAAELPVYDALYAWCQQQTAAGAAGSGG"
     gene            9759..10907
                     /gene="chrA"
                     /locus_tag="VIM45_03040"
     CDS             9759..10907
                     /gene="chrA"
                     /locus_tag="VIM45_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014618674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HEY8820295.1"
                     /translation="MSLRDLCLYFLKLGALGFGGPIALAGYMRRDLEEQRHWVTDDEY
                     QDGLAIAQTMPGPLAAQLAMWIGYIRYGVLGASLVGVLFILPPFLLVLAVAAIYDAVE
                     GLSQIRALFYGIGPAAIAIITLAAWKLAKTTNKVDPKLWAVSAALMAITIITHSELAY
                     LFIVAGLLGILVYARPKRWPRWRVRRKPRLAGLVPFAPGSIGFLPVISGTTLVAVGLF
                     FLKAAAFTFGSGLAIVPFLHQGVVVEHKWLTEHQFLDAVAMGLISPGPVVIMATFVGY
                     LAAGFLGAVVATIAVFAPVWVFNVVIGRIFLRYRQNPQVRGFVKGVTAAAVGAIAGAA
                     VLLGRGAITDVWTVVIFAAALLILYVRKLKEPYVVGLAGLAGLLLYSR"
     gene            11282..12016
                     /locus_tag="VIM45_03045"
     CDS             11282..12016
                     /locus_tag="VIM45_03045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8820296.1"
                     /translation="MVDTSVQPPDSPRDPLRLTGLYRSAVATYRARFARVAGIAIAVF
                     LVPALLSSGIDQWAEHHGRHLPGVATVLIIAGVIGAGMSVFSATFYAGVLDEAVGEYQ
                     HGHPRRSIADLLRRLPYVRLIVASLLVSVATGVAALAFVIPGMIVYTFLALTGPLINI
                     EHLGVVAAFRRSFYLVRPKFWLTLCAVTVPVTIESWLDETGDLIFHGAPWLAALALDG
                     LLAVTLLAGIGLIEVTLTYELIERDR"
     gene            complement(12013..13704)
                     /locus_tag="VIM45_03050"
     CDS             complement(12013..13704)
                     /locus_tag="VIM45_03050"
                     /inference="COORDINATES: protein motif:HMM:NF018805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HEY8820297.1"
                     /translation="MHVQAPPDSLDSLDEDTRESVRQALWLDALLPEDLPGGIQDRLV
                     SPENSNRAFGRRMQRRIREMLAAPDSFTPSYKQRYETLLRNCDTLSAHQAYAMSVLLG
                     QDNAKGYSPIPDVAGLRFPDAHPEDLQAQVGWYFCVGSCSDRKGKEYGVEVMFFRYAL
                     LPPALARQFKLSDIENQVVELHFAISVAGGRHHRMKPIVVAGTTGLIDYTTKPVSARM
                     GKNVIRSLSETDLFPLRVEARGTCHEPDGSVEVGVELTLSSEKPPLLQGANGCSPCCG
                     GVGTLYYSIPDLLLDATSSTLSINGDQVELVDGKFWFDHQWGTGMIPAGSPRPAVLRA
                     AGSLAEPGAGGWDWFMAHFSEGRALTFYSLHANEFRDFYSRSGPTAPGTMRVPISGKY
                     MDPSGTVTEVAGVMEITRWIRSVTTPDAEQYPATRTWHPDRWEFTLEDIPEDLRRFNM
                     VPIVSTGQSGFFAFGAQYSEGAVYLQDEQGNDVGRGFAESVLYADARRTIVTLAGLPD
                     TETMMKTFEPERPSRLAKLWATAYRVWPPNAKKLKQTLAQCLANGLQAPAPKPRR"
     gene            14188..14883
                     /locus_tag="VIM45_03055"
     CDS             14188..14883
                     /locus_tag="VIM45_03055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8820298.1"
                     /translation="MRTKPSAQLVVEAPCGTNVPPALTQIKMLCDAPAVVASVGAPTV
                     PKYSAMSMCDELLNCQTLVTIALGWTIATSVATGVAAPKLVGRTTSSQPGGRLVVPAT
                     NTDPAVATQPLAVKTNPAGQVLNVCAGRQPAEVRTKPTGHPPIADTGTQPPLVKTKPT
                     GHPPITDAGTQPPLVKTKPTGQGPTWASTGRVPKEAEPKMPAVTRIASSNSLIGRAHE
                     SLMSASTASYVIE"
     gene            complement(14972..16141)
                     /locus_tag="VIM45_03060"
     CDS             complement(14972..16141)
                     /locus_tag="VIM45_03060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8820299.1"
                     /translation="MTEQEERIDRIVREFDDQGWHRTGTEVDRRSAAWLAERVRKCGA
                     VAELEPFSLSRVVPRSAYVEVEGRRIEGLPLFDGGFTPPEGVSGSFGPLGSSTEIGLT
                     EGPPNAAAAGPLGDARRSAQHQAIVSITRGRSPGLAPSNARTFTEPFGPPVLQVSSEE
                     GGWLTERAAIGTPARVVAHAEREAAEAFNVVACVAGGQPDIAPLVVMTPRSGWWQCAS
                     ERGGGMACWLESMRSVAAAPVERDVFFVASSGHELGHLGLESYINHRSTLAAGALAWF
                     HFGANIGASYGPGGRLATSDDELERLALRELSEREAGSVTTAPRGWVPNGEAHNIHIR
                     GGRYVSIVGGNALFHLESDRWPDAINTGAVARYATSFARLITGLASGAIRMETAA"
     gene            complement(16206..18065)
                     /locus_tag="VIM45_03065"
     CDS             complement(16206..18065)
                     /locus_tag="VIM45_03065"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8820300.1"
                     /translation="MASDPGYWNHLRAGRLTRRRALIGAGGFSLAAAGITACSGSTNH
                     KAVSLSGTGTSGAKQNASPALGSNRLLAPPGDTSASAVPGGIYQGYAEQDIEGMDPHA
                     AGSVQTITVANFSYLRLLKKKTNFSGISTNEPVGDLAESWEFSPDGLQLTMKLRQGVI
                     WDRRAPTNGRAVDAQDVKFSVGRFLAKSPYASNYFNSKSKAAPITSIEYPDTATLVMK
                     LAFPYVPLLATLSRALNIWVIPHEADGGFDPKTDARGAGPWILDSYRPSVGLEFKHNP
                     DYYVKGRPFLDGWSMPIVPEYATQLAQIRAANIWGMVPKQEDVLQLKKDLPQISMYQA
                     DYGTDSPLLYFGFQGPFKDLRLRQAVSYMVDRQLVADTISDSAKFEAAGLPKAVRINS
                     HIGAGWPEWLDPFGKDFGPNAKYFQENLVEAKKLVAAAGITGKPSVKFYYPMNGYNAV
                     YQQTVQIVAQMLNQTGIFDIKLMPVDYQTEYIPKIHFGGDHAGAWDGISLTPAAQGDD
                     AGHQLQVQFHSQGAATRQPPGEDPKLDQMIDAQLRETDPKKRAQLIQDTQRYLPETMI
                     ATPVFYQAAGYGLTWPWVGNAGTARSSVTGPVPAAETYPYLWFDKANYSKNKP"
     gene            18415..18858
                     /locus_tag="VIM45_03070"
     CDS             18415..18858
                     /locus_tag="VIM45_03070"
                     /inference="COORDINATES: protein motif:HMM:NF015898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HEY8820301.1"
                     /translation="MSDELKLTVFRSARPGIRKVLGDLEADVMEVIWLKPAGEGTTVR
                     EVFEALYDRRHLAYTSVMNTMTRLAKKNVLKAAKDGQAYVYFPTLTQDEFVSRFVRRI
                     LEDLFVAFSGPTAAGIRALSNPLAAKRAEEFVADIARRRSTEEPG"
     gene            18859..19833
                     /locus_tag="VIM45_03075"
     CDS             18859..19833
                     /locus_tag="VIM45_03075"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HEY8820302.1"
                     /translation="MHPLLGIGSLVGVVLVGVLLLSSLRRVRSWSRRRDLEFILLVMP
                     MVSLALSVAGLRFGAGSLCFLGPLPVDYRVNIALALFMGLMAVAGLAGGVLRLVLLQR
                     TFAARGQAAQPWLAAEITRLAGPLGISQPAVRIVVHDRPLALTYGTRRPTLLLSTWML
                     AQLDKQELSAVLAHELGHVVQRDCAMVWLATVLRDAFWYLPTSRIAYRQLQQDKEPAC
                     DDFANDITDRPLALASALAKVWRHAALAPAVGMAQSLTGVDTSIETRITRLLVDATPG
                     VASIRHHRRTLAAGGVALAALVTLAVTNAVVLLAPMGCGSMAPLGKLL"
     gene            19908..20531
                     /locus_tag="VIM45_03080"
     CDS             19908..20531
                     /locus_tag="VIM45_03080"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEY8820303.1"
                     /translation="MALAITTLVLVVIAIWWLNAGTPLPGRSRTSDATGQAVSDGAGG
                     YVAFAVPANHSTLASVPAPKIGALAPDFTLPGLDGTPVRLSDLRGKTVLINFWATWCG
                     PCRKEFPELVKLVQQQGDRGLVVLAVDVSESRDDVARFAQEFGATFPIVLDSDSSVVQ
                     SYRLIGLPTSLFVDRDGILRAQQLGPLSEASLTAKLGQAGLHLSIGR"
     gene            20541..21266
                     /locus_tag="VIM45_03085"
     CDS             20541..21266
                     /locus_tag="VIM45_03085"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HEY8820304.1"
                     /translation="MELAGVNILVAFLAGLVSCISPCVLPLAPVYVGSLAGNMAAAGS
                     VDRRAPVMHALVFMAGFGLLFIALGLSVGLVGYALRDQQPLLQKLGGIALIVMGLHMS
                     RIIEIPALYRGIGLNWGTGGTPGYLRSFLAGISISAGWLPCIGPTLGTILTLALTSGA
                     VVQSGILLLVYALGLAVPFVGIGLTLSRMPPAVRFLQRHHDLIGLVAGLLLIAMGIVV
                     FTGTMARLNAAFGFTSSGLGAKL"
     gene            21304..21480
                     /locus_tag="VIM45_03090"
     CDS             21304..21480
                     /locus_tag="VIM45_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8820305.1"
                     /translation="MKHPIRVVSILGFVLLLPLLISACGTNKGSSGPAVSELNKVTSI
                     KRGEPALVFVYTDG"
     gene            21481..21723
                     /locus_tag="VIM45_03095"
     CDS             21481..21723
                     /locus_tag="VIM45_03095"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEY8820306.1"
                     /translation="MPCAQSAPVVDGLEQDYAGKLQVIRLNFNDTKNAKALQTLNVSS
                     HPTVVLIDRKGTPQGSLAGIATDAKLRPNIDALLSQ"
     gene            21839..22471
                     /locus_tag="VIM45_03100"
     CDS             21839..22471
                     /locus_tag="VIM45_03100"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY8820307.1"
                     /translation="MPCTAVGRPQRGHRLFAALYDRVTAASERKFVVRHRAQLLGLLD
                     GKVLEIGVGTGANFPYYPPAAEVVGIEPDPFMLRRAEVRLARSGRPNITLQSGSAERL
                     LFADQSFDHVVSTLVLCTVADPLQALAEIRRVLKSGGALHIIEHVRAEGWLGHGQDLL
                     RPAWQFVGAGCVLNRRTAELIQAAGFRIETLVPYRMAFGIPMLVGMARPT"
     gene            22504..22650
                     /locus_tag="VIM45_03105"
     CDS             22504..22650
                     /locus_tag="VIM45_03105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8820308.1"
                     /translation="MRQRLQTLNDSSHPTVVLIDRKGTSQEPLAGIATGAKLRPKIDA
                     LLSQ"
     gene            complement(22728..25829)
                     /locus_tag="VIM45_03110"
     CDS             complement(22728..25829)
                     /locus_tag="VIM45_03110"
                     /inference="COORDINATES: protein motif:HMM:NF017262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HEY8820309.1"
                     /translation="MTSSRYGDSARWARLRMPWWASRCVVFLVAGTLLVLGGHPSAAL
                     AHAVLVRSDPPQNARLTASPQRIDLFFSEPANHSFSTIQVFDSSGARHDKRDVRFTDD
                     PTEVTVSVEPLGPGFYYVNWVTVSDVDAHRWQGTFPFTVLNADGTAPAGIARPPTEGG
                     GSSALQPPDAVLRWLLYFGLFGITGGFGFAALILFPAAGALAGSDRLKARQFTLWLLG
                     AVVPAALILVAIINAAVLLRQAEQIGSLGALRDLLSGRTGTYWLAREALALIAGGLVW
                     WVTRTARRANDRLTAALLWAGLVTGLGGLLTMSLTSHAAAGIGSDWSVLSDFLHLTGV
                     AFWLGCLVQLPAVLSMGPGLSNPIRRRLQSSALRRFSILAACSVAIVLLSGTFNALVQ
                     IASPAALKDTAYGRTLLVKLAFVLPLLGIGLLNSVRIAARFERLSHRNDDQTEQQGRR
                     LARVIVLESIAGAIVVAAAAVLVFLVPARNPVASANGRQPAAAAASSVYSRETSAGDL
                     RISLGVNPNRIGDNEFRVQLASPGVDVAQRVQLRFQSAKEQTGGSTVTLDPVAGSPGL
                     FAASAANLGVGGRWEIRVNVRRAQHDDVEGAFRVTVPGSSGAAAIDATAYPAHGITPA
                     QGAAAVLLATAVLLAVLRWRFGFPALPPRTVALAASTTALVAVAVFVVSLPSSGSRTG
                     AGGELQAAAGPVASPSTGPGAAAAGRTGRPVTTTVAPVRSADYSSWKVPTSGSGLMIP
                     AIGSDGRIWIGEMTANKLAVLDPSTNTYTEMAYPPGRSIGSMGVVVDAANVVWLAQSG
                     VSALGRFDAVTGDYREIPIPTPNSNPSGIALDSHGRVWFTELAMHKVGVYDPAARTFA
                     EYPVPDAKFLPYWLHIAPDGRVWFTDIASASIGVLDPASGRIDITSVPGTKRTTGIDV
                     AADGTVWFGANAEGGKLGRLDPHNGQVSLLALPGGDAYGVTLDANGVIWVGTTGNTVY
                     AFDSTTQQFRTFRTAAGPWWPAPAGDGSVWIAEAADDGNALVRISSVAASAGTSTVVG
                     TQP"
     gene            26202..26726
                     /locus_tag="VIM45_03115"
     CDS             26202..26726
                     /locus_tag="VIM45_03115"
                     /inference="COORDINATES: protein motif:HMM:NF023095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HEY8820310.1"
                     /translation="MNLKSCLNWKVIGGLAVVGAGIWIVQPGLISRALPLLLIAACPL
                     SMMFMMGGMGKMMGGSKQDGATGAYTCPMHRDVQSDQPGTCPKCGMSLVPTAAPTRQA
                     QATPMGGTSIAPAQGANAAQLRAQLQDLTAQQQVLAQRLEELDAATETAVPSKVLRDA
                     EAVAGAADADNRHP"
     gene            26803..27180
                     /locus_tag="VIM45_03120"
     CDS             26803..27180
                     /locus_tag="VIM45_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8820311.1"
                     /translation="MTQQEVWDLWYPNAAAQGLSFARGRLDSTDVLLVHAAPDSLRVQ
                     VSDDAGKQLAFGDQLRRTADRPMTRLRRVGSTITREDLWPEEQDIGRPVIVPGGEVGI
                     IRSWWNAADGSEWRWQVEFYNRR"
     gene            27413..28147
                     /locus_tag="VIM45_03125"
     CDS             27413..28147
                     /locus_tag="VIM45_03125"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY8820312.1"
                     /translation="MAEQLADEARIFCPDLLGFGRAPRPSAAYSVADHLAALDACRDS
                     LALGSEPVILAGQSAGSVLALAGLPPGRSGLPASGCWRCLLIRRSKMPGDTSVPFPRW
                     HRRPWTAPTSEGSSVSGLMCVGRPLWQRLMPLVMRQFPADMARDMVLQDRVSYRNTCN
                     NVLVDHRCAPSAAQVAKAKIPVQMLHGSKDKTAPLAHVQQLAGQFGWPLTVLEGRGHR
                     LLVEAPHACAGTIRTLLADNMAIAGH"
     gene            complement(28248..30509)
                     /locus_tag="VIM45_03130"
     CDS             complement(28248..30509)
                     /locus_tag="VIM45_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017617916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEY8820313.1"
                     /translation="MSTRTEVRSLDSPDGLPAPADTTQKLQLKVGGMHCSLCTDSIRR
                     SVGRLDGVKDVQVSIAHEETLVEYDPARVDPLTIQAALEDIGYTVREPDQAERFAEEE
                     HDLAMARRKAFLSVGLLLAASALMVFTVVAGANLYRSLAMGVLAAFIVLGPARFIIAR
                     NGYQSVRRGILNQDVLVTSSALGGLLGGVVGLFVHRLPAGGFFGATVFVLSFHLIGGY
                     ASVLVHVRASQSVRRLLALEPPTARRVQPDGSEVEVGVEQLLVGDAVRVRPGERIPID
                     GVVTEGASAVDESLVTGESIPVDKLPGSEVIGGALNQAGSVVIRVTRVGSDTFLRTVA
                     RHVAEARALKPGILRLVDRVLLVYVPVVFAAAAAGALIWTLGAWAFAGEPNWPRASFA
                     ALSVLIMGYPCALGMSTPLAIIRASGEAAERGILMRSGEAFHVFKNVRTIVFDKTGTL
                     TEGKPELVAYFSPAGDAEELLQLVASAEALSEHPLGQAIVDEARRKNLLLTPTTSFEA
                     RPGRGIIASVRGQRAVVGTQRFLSEEGIDTTPIAALVEQVQARGQTAVLAALDGGAAG
                     VFALADRLKPDAQSAIARLRTLGITSVLLTGDNWRTGRAVAEAVGIEVVLAEVLPAEK
                     AAEVRRLQAQGNKVAMVGDGINDAPALMQADVGIAIGAGTDIALEAADVVLVSERLGT
                     LVEARELSRRSYQLTVTNVTLALAFNGIGVLAAVTGLVQPVWAMVAMAASVSVVLGNS
                     FLGRLVSRRVTDG"
     gene            complement(30506..30742)
                     /locus_tag="VIM45_03135"
     CDS             complement(30506..30742)
                     /locus_tag="VIM45_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018991681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HEY8820314.1"
                     /translation="MTQTIQFAVTGGDTIHCESCEQRINTMLRRLTGVERVQASHETQ
                     QAVVTFDPDQVQADAIRTKLAQGGFETALQGETT"
     gene            complement(30838..31347)
                     /locus_tag="VIM45_03140"
     CDS             complement(30838..31347)
                     /locus_tag="VIM45_03140"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-responsive transcriptional
                     regulator"
                     /protein_id="HEY8820315.1"
                     /translation="MSEQLLSAEMRIGELAGQLGLNPKTVRYYEDIGLLPRPRRTPAG
                     YRLYDAADRDRLQFIVKARAIGLTLEEIGHILALRGEGQPPCVHVLGLLDQKVAAVDQ
                     HLRALTAFRQELAALRDEAAKSVDRDAPVCAIIEHHELATKHESPCNAITPIGTIRRK
                     GQARTPRGS"
BASE COUNT         5575 a        10074 c        10046 g         5930 t
ORIGIN      
        1 ctgtattatc gcacggcgcg ccggctgggg ggcgatgagc gcggcatgcc aacggctccc
       61 attccgtcgc aagcacacca tcgagaggcc gcgcgaccca gccctcacgt cctgttcgca
      121 agtggtacgc ttggccgcgc cggccccata attcgggtgc ggcgattagg agcgagacga
      181 tggcaggacg tgtcgatggc gaggtcgcgc tcatcatggg cgccgggcaa acccatgttc
      241 aggtttgtcc gaaagtccat ggccacgcat atcggagcga tgatggtgaa ctcgtcaacg
      301 gcaagcgcct cgaatggaac ggccccacgc gcaggcgccg agctatgtcc gtactgcagc
      361 aggctggtat atgggccgct tccggcccag aggtagtgta gagatacacc actcacgcgc
      421 gggcagccaa cggcgatcaa caacgaatgc acacgtcaga caggctctgt agagccggtg
      481 attcctaacg catcgagttg gtcgtattcc tcatccgtcg tcgccagcag ccggcgaaag
      541 acgtcccggc tgtgctcacc gaacagcggc gagtaccgaa cccgtgttcc gggtgtacgg
      601 ctcaggcgcc agggaaccgt cacttggtcg tggatgccgg cctcgggatg ctctatcggt
      661 tggagcatac cccgtacacg aagctgctcg aatacgagca catcgtccgc gttgcgtacg
      721 gcgcccgcgg taatgccggc ctcatgaagt accgcctcca actcaaaggc gtcccgagtc
      781 gaggtccatg caccgatctg ttcgtcaagc gctgtttcgt tacgcttgcg gtcggccgca
      841 cctatgaaac gtttgtccaa tagccagtcg ggacgtccag cagcggtgca cagccgggaa
      901 aattcggcgt ccgtgcttgc agccagggcg atccaacggt cgctcccggt gcacgggtac
      961 agattgtgcg gcgcgatggt cggatgatgg ttgcccatgc gcccacgcac gcggccattg
     1021 gcactatagt ccatcaatgc gtcggcgatg aagcacatgt tagcctcttg catcgacaga
     1081 tcgatgtact gcccgcgacc cgtgcgctcg cggtagtgaa gcgcgatcaa aagggctgcg
     1141 gccccgtaac tgccggctac ggcgtcgggc accatattac ccgagttaat cggggggccg
     1201 tcttcgtatc ccattaaggc ggacatcccg gccatcggct cgactgtgcc accgatccct
     1261 gggaagtttg agtagggacc agtagccccg tacgccgaca gcgataacat gatgatatcc
     1321 ggccggattt ccaccagcga cgagtagtct aaacccaagt tgcccatcac ccgcggggcg
     1381 aagttttcgg cgacgatatc ggcggttacg atcagttgcc gtatcattct ggcaccctgc
     1441 gggctggcaa cgtctagagt gatagcctcc ttgctcaggt tgaccgagtt gtaatttggg
     1501 ttgcagttcc agccatgcat tgccgtcggg atacttcgca atgccggcgg cacaacggcg
     1561 ctgtatccac cgcgccagtt atcagggcgc gagcgaacct cgatctggat gacctccgct
     1621 cccataagcg agagcatctg cgttgcgaac gtacccgccc acgcctgggt aaatacgacg
     1681 gcacgcaggc cctctaatgg cccgcgcgat tccgcgccgg tgattgaaac ggcgcctgac
     1741 cgttcgctag atgtcctgcc gccacggtcc gaagtcttca gcccgccgcg tcttgtcgaa
     1801 atattctcgt caatcggccc agcgtcgctt ggtgacgact cgtgttttaa ctgtcggttc
     1861 aaatcactgc cgccgtggca agactgctga gttcggaagg ttgcaggccg attgcaccca
     1921 agacttcagc cgtgtgctgg cccaaccgcg gcgcagtttt cgatagatgc cacggcgttt
     1981 cggacatctt gaacggcgcc cctggatagg atatccggcg gccgtcgaca tcgtctgact
     2041 cgaccaagaa gcggcgctct cgcacatgcc ggtcggcgaa gatctcctcg gtcgtcaaca
     2101 ccatgccccc gacgacgcgc agcgtaccaa gcgcttcgaa caactcttgc ttctcccggc
     2161 cagcaatgcg cgactcgacc agcggtgcta cgagcggcgc gtgttctcga cgtgacttcg
     2221 actcgtagaa gcgctgatcg tacgccaggt ccggtaggcc caattcattc attgcatcgc
     2281 gccaaaagtg tgcccgactg agagtcaagg caaaatagcc atccttggcc ggcacggggc
     2341 cattctggaa attgggtgct ctccgtccgt gcggtgcccc ctcgtactgc gcttgtagca
     2401 cgatcggggc aaatgttgcc agcagtggct caaggatgga gacgtccacg tgttgaccct
     2461 taccggtatt acggtctcta tacacgaccg cgttcatcgt cgctacgaac gccagtaggc
     2521 cggcctgcat ggagccctga tagccgctcg ccttgagtgg ctctcgattt ggccagccat
     2581 ttatcgacgc ccagccggaa catgcgtagg cgacgaggtc attgattggt agatcacgcc
     2641 acgggccagt ctgaccgaac ggtgtaaccg aagtcagaat gatgccgggt cggatgcctt
     2701 ccatggactc ataaccgaga ccgagttcgt cgagcattcc gggcggaaag gattcgacga
     2761 taatgtccgt gtcctcgatc agctggcgaa ggatcgccat cccgctgggc gttgttacgt
     2821 ccagcgtgac gctgcgctta ttcgtgttca ggaatagaaa gaaggcgcta cggtctggtc
     2881 caggtacatc gtcaataaac ggaggtagcc gccggccgga ctctccgccc ggcggctcga
     2941 tcttaatgac gtcggcaccg aagtcggcca acattttagc gcagtacggc cccgccacgt
     3001 ggccggagag atccagcacg cgaaggtcat cgagggcggc cacgtggtcc tcctggtttg
     3061 caaagctgag gtccgcgggc acctcactgc ccagtgaaag ccgcgggcct acgctcccgc
     3121 aatgcgagtt tgccctcggt cgagtcctta gtctcctgcg acagcttcga tgagagggcg
     3181 ttaattgcaa acgcggtttc gttgtcggaa tagagtccgc gatgcaaggt gtgtttagcg
     3241 attccgagta cgcgggttgg tccggccgcg agcttgcgcg cccaagcctc gacctccgcg
     3301 tcaaacgctt ccgccggcac cgcgcgattc accaggccga tgcgttcggc ttcggtgccg
     3361 ttgaccgtct caccggtcaa caccatttcc gaagccctcg ttagtccgac gtagcgtgta
     3421 accagcgcat tgccgccggt gatgccgatc ttgacgaaga cgaagccaaa gggcgcgtct
     3481 tccttcatga tgcggatgtc ggccgccagg gcgatgaatg cgcctgcgcc gagagcattg
     3541 ccgttgatcg cggcgatcac tggcttgcgc aggtcgtaaa ttgtcttgaa gagtcgttcc
     3601 cagccagcct cgcgtcgcag ggtatactcc atcgtgtccc actcaggcga accgtccgac
     3661 atgcctttga cgtcgtcgcc agcgcaaaat gccctgccac ggccactaat cacgatgcag
     3721 cgcacgttgg ggtctcgctc ccacgtcttg agctgttcgc tcgcctgagt gatgaggtcg
     3781 aagtttagtg cattgagacg atccggccga ttcatccaga gccagccgac accattctga
     3841 tcgacaggac attcaattac gttgcttact gcggtcatcc tagtgatact ccttccattc
     3901 cttgccagtc cgcgcctttg ggcacgaacg tgcccttaat cggccccgcc cacgccacat
     3961 agaggttctg gtagaccgtg tgcaggcggt acaggtagtt ctgccagtcc ttggtcatct
     4021 gcttgatgta cgcattgcgc tccttgacat ccagcatccc gcgctctttt tcgatgcgtg
     4081 cgtccagttc gggatcagaa gcgcggcccc agttcttacc gcctttcgag tggtagtagg
     4141 ggaacagtag actgtccggg tcgggtcctg cgccggaccc gatggtctgg atcttgaagt
     4201 tgccgtcgac ttgatgttgc aagtaggccg tgcgttccgc cggcttgaga tcgatattga
     4261 tgccgatctg cgccagcatt tgcttcatca gaggcatttc gtcaccggcg agattatcaa
     4321 tgttggaata ctccatcggc acggcaaggc tgtcgacgcc ggcggcggtc atgagctgtt
     4381 tggccttttt gaggtcctgc ttgtataggc ccttgacctc gtcctgcgac aatgccatgt
     4441 cgccgtagcc ggggggaatg caaggcccgc cccaaaagcc atctccctga acgacatttt
     4501 gcagaatgac atcgcgatca atagcgaggt cgaacgcctg gcgcacgcgc acatctctga
     4561 actccttctg accattcggc gccacgttga aaaccttatg gctggccgag aaggagccat
     4621 acttagtgat ttgcgcgccg gtcttcttga cctgatcgac gtcctgtggt ccggctagct
     4681 cgtggtacgc gactgcaccg gagaggaaat tagccagacg agccgacgcg tcgtttatca
     4741 cctttatctc aacggcgtcg aggtagggta gaccggggcg gaagaaatct gggttccgca
     4801 ctaggagcct gtccggctaa cacttgaacc aactcggtgg accggcctac gcgtgcgcta
     4861 gcgtcggtag ttttggcctt ggcgaccgtt ccctttggtc ggttgggccc tctgcaccac
     4921 gtgggaaccc tgccttccgc ctggcttccc tggcttttgg ctgcgtcccc ggttcaagtg
     4981 atggcggcac cgcatcgata gatacgaagc aggttgtgca cggtgcagag cagcgcccat
     5041 tctccttgcg ttgccgtgag tccgcggagg aggaactgcc ggaacccgcg acatgccttg
     5101 atgtgtccga agaccggctc gaccgtctgc ttccggttgg cgtaggcggc ccgccccgcc
     5161 gcggtcccca acttctcccg catcctcgcc cgtgccggcc accggttccc gctcggcctc
     5221 gcctcggcga cctgctccgg cggcgcgtca tgcttcagtc gccccaacgc cacgtaggcg
     5281 tcaacgccca ggtcagcaca gtgctgcagg ttcgcctccg atgcataccc cgcatccgca
     5341 ctcacctgct ccggataccg ccccgtctct tccttcaccg tctccagcat cggtgccagg
     5401 cgctgcacgt cggtcggcgc cgctgtcagt tcctgcccga cgatcaccat gctgtcggcg
     5461 tctaccgctg cctgcccgtt gtagcactgc tggaacccgt ccggcgtctt catgatccgc
     5521 gactccgggt cggtgaagtt atactgcgtc ttcgccgcca cgacggcctg ctccgggtcg
     5581 ctgccctccg ccttggcctt ctcccgagcc cgctcctcca acgccgcttt cgccttgcgg
     5641 atcgcgacgg cccgcttttc cttcgtcgct aggtgcgccg gcagttcatc cccttgacgg
     5701 tcccccaata ctgcatcctc ggccgcgtcc gtctcctccg cctgcgcaag ccagccggct
     5761 acctccgctt ccagggcgct ggcccgctcc tccatccgcc catagctcat ggctttgtgc
     5821 cgcgacgcgt tcgccttcac cttggtcccg tccagggcga catgccccag cttcaccagc
     5881 ccggcctctg cggccagctg cagcacctgc ccgaataggg cgccaagcgc gtcaaggtgc
     5941 cgcgtgcgga aggcggcaat ggtgcggaaa tcgggcgtct gccgtgcggt aacgaccatg
     6001 aagcccagat cgtggctgca ggcgaaggcg atgcggcggg agctgtagac gccccgggcg
     6061 taggcataca acagcagcgt gaccagcatc tgcggatggt agggcgggta accacgcatt
     6121 tcacgctcgt acggcgcgaa gatggcagac aggtccaggc cggcaacgac ctccggcacg
     6181 aagtaagcga ggtcaccctc cggcagataa tcctgcaggg aagcgggcag cagcagcggt
     6241 tggtctggct ggtacggccg atatgtcttc ttcatacccg catgatccca gacggcgcgc
     6301 atcctgtcga tccctgttac tcggacaggc tcctagtgcg gctagacgga cgacgtgatt
     6361 gacgaactcc ggcggcgctt cggctgtccc gagcaggacg ttctctcgta ccgaaccgga
     6421 gaacaggtgg gcatcttgtg gcaggtagcc gattgtgcct gcccggtggc tgatgtccag
     6481 cggcgtgcca gcctccccag aaaggacgag atcacctgat tcaattgggt agacacctag
     6541 caaggcgcgg gccagcgcac tcttacccga acctatcggc cctgtcaccg cgacaaagct
     6601 gcccgctgga atctccagcg aaagatctga gagagcctgg tcactcgcgc cgggatagcg
     6661 gaatgaaagc ccacggagcg acacgcccac aggaccggag tcgcgtctcc ctggctgttc
     6721 gtcctcgcga tcaataccgg caacgcgttc cgggttaaag gacgcaagag gaggctctcc
     6781 ttcgaccggc aggggaggcg cgagcagggg ttccagacgt gcgtaagcgg cggcaccact
     6841 ctgaattgaa ttgacgagct gcggcatgcg gtagccgcgg tcgacaaagc gaatgaacag
     6901 gtttagatag ccgacgaacg tacccagagt catcgcgcct ccgacaactc gctcaccgcc
     6961 ctgccagacg acaaagagca ctcccgccat catcaacgtg ctgtaggcgg gctgcagtcc
     7021 cagtctgaga cgtgcagtgc ttaagtttcg cgctgcgtaa ctttcggaca gagctgcaac
     7081 ctcggcggtc gccgcttggc cgcggccgaa aaaacgtagg acgcgaaagc cggccaacaa
     7141 ctcctggatg ctggcggtaa ggtccgcgtt ggcctctctc gcgtgagtcg tacgggcgga
     7201 aacccaacgg cctgaagaat gagctatgag catcgctacc ggcacaggcg ccaaggcgat
     7261 tgctgttagt cctgggttaa ttacgagcat cgcgaccacg aacgatagag agaacagcac
     7321 cgtgtcccag gtctcaatcg tgaactcgcg gacgcctatt cccagcacct cgacatcgcc
     7381 gacgatcctg gccatcagct cgccgattga ggttcgatgc aggtccgcca gcggccgcga
     7441 tagcgtgcct ctgagggcat ccgctcggat gttgcaacgg atgcggccat tggcggtcat
     7501 gagccaccag cgtttgccga tccgcggtat ctccgtaagc agagtgccgg cgatgaggag
     7561 caaagcggcg accgttacct cccatcccgt cgcttcgccg cgttcaagag cgagagcctt
     7621 gtcgatcgct cggccaagga agattgccgg ccctacgaca gcagtgttca tgacgatgcc
     7681 gcaaacggag ccgaggacaa gctcgcccgc cacctggcgg aagtaggggc gcattcgcca
     7741 gagataggtc atcatcgccc ctcgcaacga tcgatcgatt cctgatccca ttgtggctca
     7801 gcgcccaaat gcgagggcgg cgtcgtttga actgaggcta gatggtgcgt ttgcaacgtg
     7861 gtaaacatct ggttcatcac tgctggttgt acaggtaatt gttgaaccgt gcaaccgcgg
     7921 ttgcatcccg ttaatgaagc gactatagtc cggatatgga tgcgaaccgt caatgggtct
     7981 tgctggcgta ccgcttgccg cgagaaccgt ccacgccgcg tattacgctg tggcgaaagc
     8041 tccggcgctt tggcgccgtt caggtgctgg acggcctcgc cggcctgcca ctgacgccgc
     8101 gtacgcgcga acagttcgaa tggctcgctg acgaggtcat tgaggcggac ggcgaggcgt
     8161 ccgtgtggat ggccgctccg ctctcggccg cggaggagcg agatcttact tcacgcctga
     8221 cgagagccat cgttgacgaa tacagtgcga tcaccacggc ggcacaagcc gctcttcgcg
     8281 aaccgtcgct ggcgcgccgc cgaatgctcg cccgactgcg gcgtgaacta cggcgtatcg
     8341 acgaaagaga ctacgttctt ccatcagcac gaaacgacgc gcacgcggtg gtggccgcgc
     8401 ttaccgctac gctggaggtg cccgcatgaa gtgggctact cgccccggct gtcacatcga
     8461 ccgcgcggca tgcgcctggt tgattcaacg ctacatcgat ccggccgcgg cgttcgtgtt
     8521 cgtggaggat acggccgggg taccacccga tgcgacgccg ttcgacatgc gcggcgtcga
     8581 tctgtcgcac catgccggtc gctgctcatt cgagaccttc ctggtgcggt atcggctcga
     8641 cgacccggtc ctgtgggaca tcgctcatat tgtgcatgag gcggatctcg ccgacgaacg
     8701 ctacgacgca cccgaggcgc ccggtctgga cgtgcttctg cagggactcg tgctcgtccg
     8761 cggcgacgcc gacgtgctcg caattgcaat gacgctcttt gatggcctct acgccatccg
     8821 caagcagctt gcgctcgcga cctcgttccc acgctgagga gcgcacgaaa ggctcgcatg
     8881 tttgccgtct ccgacatcct ggtcgacgcg ttaatcgcgg gtgtgctcgc gggggcgctg
     8941 ctgttcgtgt ggccgtggac ccgccggcgc gggtgcttcg ccgtagccgc tatcagcaca
     9001 ttcgcggggt ttacggcgtg gaacctggtg ctgaacagca cccacggcag caacttcaac
     9061 gtcgatgcgc cggtgattgg cttgagttgg gccgacgccg gtagcggcgt cctcgccttt
     9121 gttgccaccg cgttggtgct aggtcttgcg ctcgaacgaa cggaacccgc cggtcgtgtt
     9181 atcggcgccg ccgcgattgc tggggtcgtc gccatgggcg tcgacctctt tgtgctgtag
     9241 ctgcacagaa aggacgtagc tgatgaagtg ggtcactcgt gcaaggccaa tggtcgaccg
     9301 gattgcctgc ccctggctga taaagcggtt cgtggacaaa gacgccgagt tcctgtacgt
     9361 gcccggcgac caggtcctcg cggtggcgga gcgggaaggg gccacgccat acgacattcc
     9421 aaacgctgag cttgggcacc acggctccga gtgcagcttt gacgccatca tcaagaagta
     9481 cgagcttacc gacccagcgc tacagcagct cgcgctcatc gtgcggggcg ctgacaccga
     9541 tgcgcgaaac ctcacgccgg aatcgcgtgg cctggtcgcc atcgctaccg gcttccggct
     9601 tgcctaccag aacgaccacg agcagctggc tgccgagttg ccggtgtacg acgccctcta
     9661 cgcgtggtgc caacagcaaa ccgctgcggg cgccgccggg agcggagggt agcgcggtgg
     9721 cagcaccggt accccctgcg agtcgggaac tcacaccggt gtccttgcgc gacctctgtc
     9781 tctactttct caagctcggt gccctggggt tcggcgggcc gattgccctc gccggctaca
     9841 tgcgacgcga cctcgaagag caacggcact gggtcaccga cgacgagtac caggacggcc
     9901 tggcgatcgc tcaaaccatg cccggcccgt tggccgcgca gctggcgatg tggattggtt
     9961 acatccgcta tggcgtgctg ggcgcctcgc tcgtgggcgt gctgttcatc ttgccgccgt
    10021 ttctgctggt cctggccgtg gcggccatct acgacgcggt ggaagggctg tcgcagatcc
    10081 gggcgctctt ctacggcatc gggccggcgg cgattgccat catcaccctg gcggcctgga
    10141 agctggccaa gacaaccaat aaggtggacc ccaagctctg ggcagtgagt gcggcactga
    10201 tggctattac catcatcacc cattcggagc tggcgtatct cttcatcgtg gcggggctgt
    10261 tgggcatcct cgtctacgcc cggcccaagc gctggccgag atggcgcgtg cgacgcaagc
    10321 cgcgccttgc aggcttggtg ccctttgctc ccggcagcat cgggtttctc ccggtcatca
    10381 gcggcaccac actcgtcgcc gtgggcctgt tttttctcaa ggccgcggcg ttcacgttcg
    10441 gctcgggact ggcgattgtg ccgttcctcc accagggcgt ggtggtggag cacaagtggc
    10501 tgaccgagca ccagttcctc gatgccgttg ccatgggcct gatttcgcct ggcccggtcg
    10561 tcatcatggc gacctttgtg ggctacctgg ccgccggctt cctcggggcg gttgtcgcca
    10621 ccatcgcggt gtttgcgccg gtgtgggtat ttaacgtcgt catcggccgc atctttctgc
    10681 gctaccggca gaacccgcag gtccgtggct ttgtcaaagg tgtgacggcg gccgccgtgg
    10741 gcgccattgc gggggcggcg gtgctgctcg ggcggggtgc gattaccgac gtatggacgg
    10801 tggtcatctt cgccgccgcg ctgctcatcc tctacgtcag gaagctgaag gaaccgtacg
    10861 tggtcggctt ggctgggctg gccggcctgc tgttgtatag ccgctgatca ccgccggttt
    10921 agccggcagg tcggtggcgg tcagggaggc tggtacaatg cttccacggg gtgcgtggga
    10981 tgcgtttacc gtggcagcgg ccaatggcgg ttatctcggt ttcgtatgaa ccgcgtgatc
    11041 gcgaaacgga gtgggtagtc gtcgacggcc ggagggttgg ctacctttac cggatgtggc
    11101 gcaactgtct ggcctgcacg cccagcaggt cgcgtatgtt tgacgacgaa tacaaagcag
    11161 tgcgttggct ggaatctcag gctgcggacg taccaccagc cgggtagtct gcgctgatat
    11221 tcaaaccgtg cgcccgccgg tgaccttgcg gtcacgccgc ggctcgaact agagtcctga
    11281 catggttgat acgtcggtac agcctccgga ctcccctcgg gatccccttc ggctcaccgg
    11341 cctctatcgc agcgccgtgg cgacgtaccg ggcgcgattc gcgcgcgtag ccggaatcgc
    11401 gatcgccgtt ttcctggttc ctgcgttgct ttcgagcggt atagaccagt gggccgagca
    11461 tcacggccgt catctcccgg gcgttgcgac ggtgctcatc atcgccggcg tgatcggcgc
    11521 cggtatgtcg gtcttcagcg ccacgtttta tgcgggcgtc cttgacgagg ccgtcggcga
    11581 atatcagcac ggacatccca ggcgcagcat cgctgacttg ctgcgcaggc tgccctacgt
    11641 tcggttgatt gtggcgagcc tcctcgtctc cgtggctacg ggggtcgctg ccctcgcctt
    11701 tgttattccg ggcatgatcg tgtatacatt ccttgccctg acgggtcccc tcattaatat
    11761 cgagcacctg ggcgttgtcg cggcgttccg tcgctcgttt tacctggtgc ggccaaagtt
    11821 ctggttaact ctgtgcgccg tcacagtgcc ggtgacgatc gaaagctggc tggacgagac
    11881 cggagacctg atatttcacg gggcgccgtg gctggccgcg ctggctctgg acgggctgct
    11941 ggcggtgact ctgctggccg gcattggtct gatcgaggtg acgctgacct acgagctgat
    12001 cgagcgcgac cgttagcgcc gcggtttcgg ggccggcgcc tgcaggccgt ttgcgagaca
    12061 ctgcgccagc gtctgcttca gcttcttcgc attgggaggc cacacccggt aggcggtggc
    12121 ccacagcttt gccaggcgcg agggtcgttc cggttcgaag gtcttcatca tcgtctcggt
    12181 atcgggcagg cccgccaggg taacgatagt tctccgggca tcggcgtaga gaaccgactc
    12241 ggcaaagccg cgaccaacgt cgttcccctg ctcatcctgg aggtagaccg caccttccga
    12301 gtactgtgca ccgaaggcga agaagcccga ttgtccggtc gaaacgatcg gtaccatgtt
    12361 gaaacgccgt agatcctccg ggatgtcttc gagcgtaaat tcccagcggt ccgggtgcca
    12421 cgtgcgcgtc gccgggtatt gctcggcgtc gggtgtcgta acgctccgta tccaccgcgt
    12481 gatttccatg acgcccgcga cttcagtgac tgtccctgat ggatccatgt acttgcccga
    12541 gatcggtact cgcatcgtgc ccggagcagt gggaccgctg cgtgaataga agtcacgaaa
    12601 ctcattggcg tgcaacgagt aaaacgtcag cgcacgccct tcgctgaagt gggccatgaa
    12661 ccagtcccag cctcccgcac ccggctccgc caggctgccg gccgcgcgca gaacggcggg
    12721 ccgagggctg ccggccggaa tcatcccggt gccccactgg tggtcgaacc agaacttgcc
    12781 gtctaccagt tctacctggt ctccgttgat ggaaagcgtg cttgacgtag cgtccaacag
    12841 caggtcggga atcgagtagt agagcgtgcc gacgccgccg caacacggcg agcagccgtt
    12901 tgcgccttgc agcagcggcg gcttctccga cgataaggtc aattcgacgc cgacctcgac
    12961 agagccatcc ggctcgtggc acgtcccgcg cgcctcgact cggagcggga aaaggtcggt
    13021 ttccgaaagc gagcgaatga cgtttttgcc catccgcgcc gatacgggct ttgtggtgta
    13081 gtcgatcagc ccggtcgtac ccgcgacgac gatgggcttc atgcggtggt gccggccgcc
    13141 ggccacgctg atggcaaagt gcagctcgac cacctggttc tcgatatcgc tcagcttgaa
    13201 ctggcgcgcc agtgccggcg gcaggagcgc gtagcggaaa aacatgacct ctacgccgta
    13261 ctccttgccc ttacggtcgg aacaactgcc gacgcaaaag taccagccca cctgcgcctg
    13321 caggtcctcg gggtgcgcat cgggaaaacg gaggccggcc acgtcgggga ttggactgta
    13381 gcccttggcg ttgtcttgtc ccaataacac gctcatcgcg tacgcctggt gcgcagaaag
    13441 cgtgtcgcag ttgcggagca gcgtctcgta ccgctgcttg tagctgggcg tgaaggagtc
    13501 cggggcggcg agcatctcac gaatacgacg ttgcatgcgt cggccgaaag cacggttgct
    13561 gttctcgggg ctgacgagcc ggtcttggat gccgcccggc agatcctcag ggagcagcgc
    13621 gtccagccac agcgcctgcc tgacactctc tctcgtgtct tcgtcgagcg aatcgaggct
    13681 gtccggcggg gcttgtacat gcatgagtaa agtctccgta cgtaatggcg gcgcacgcga
    13741 aggcacctat cccaagagcc gtgagaaagg cctcgtaacg agtgccgtcc ccgccatagt
    13801 acataaagga cgccgagccg tctggttggc gctaaaagcc gcaacggggg cgcgttcatc
    13861 gcacactccc ccgttgcggc ttttatggca cctggtgcgc agagcttact cgttaaacga
    13921 gcaagtccct gtcacggggt tgaagaaaaa tccgaccgga cagccgccga cggtcgagag
    13981 cggcacgcag ccgccgagtg ccgctatgtc cacaaagccc ggcaggcagg cacccgtgcc
    14041 ggagaacggt acgcaaccgc caaaggccgg cgcaaagcca aagcccggcg cgcaggagcc
    14101 ggagaggggg gctggcaacc gctgacagtc gccccgaagc cagccgggca cccgccgatg
    14161 ctggctgtgg ggagacaacc ggccgcggtg aggacgaaac catcggcgca gcttgtggtc
    14221 gaagcgccct gcggcacgaa cgttccgccg gcgctaacgc agataaagat gctctgtgac
    14281 gccccggccg tggtcgcgtc cgtcggcgcc ccaaccgtgc caaaatacag tgcgatgagt
    14341 atgtgcgatg agttgctaaa ttgccagacg ctggtcacaa tcgcgcttgg ctggaccatc
    14401 gccacgagcg ttgcgacggg cgtagccgcc ccgaagctgg tcggtagaac cacctcatca
    14461 cagccgggcg gcaggctggt tgtaccagcc acgaacaccg atccagcggt cgccacgcag
    14521 ccgctggccg tcaagacgaa cccggctggg caggtgttga acgtctgtgc cggcaggcag
    14581 cccgcggagg tgaggacgaa gccgacgggg catccaccta tcgcggatac cggcacgcaa
    14641 ccgccgctgg taaagacgaa accaaccggg catccaccaa tcacggatgc tggcacgcaa
    14701 ccgccgctgg taaagacgaa accaaccggg cagggcccga cctgggcatc gacagggcgc
    14761 gtgccgaagg aagcagaacc aaaaatgccc gcggtgacca gaatcgccag cagtaacagc
    14821 ttgatcggtc gcgcccacga gtcgctcatg tcagcctcca cggcatccta tgttatagaa
    14881 taggtggctg cgcggggaag tacctgagaa tatcctcagc aagagcgcaa ggcgccgcgc
    14941 cgaaagagtg caatttgcta gtctatctag tctatgcggc tgtttccatt cgtatggcgc
    15001 cgctggcgag gccggtgatc aggcgggcga aggaggttgc gtagcgcgca accgcacccg
    15061 tatttatcgc atcgggccag cggtcgcttt cgaggtgaaa cagcgcgttg ccgccgacga
    15121 tcgagacgta gcggccgccg cggatgtgga tgttgtgcgc ttcgccgttt ggcacccagc
    15181 cgcgcggcgc cgtcgtgacg ctccctgcct cacgttctga gagttcccgc agcgccaggc
    15241 gttccagctc atcgtcggaa gtggccaggc ggccgcctgg cccgtaagag gcgccgatat
    15301 tcgcgccaaa gtggaaccag gccagcgcac cggctgccag cgtcgaccgg tggttgatgt
    15361 acgactcgag gcccaggtgg ccaagctcgt gcccgcttga ggcaacgaag aaaacgtcgc
    15421 gttccactgg ggccgcggcg acagagcgca tactttccaa ccagcacgcc atgccgccgc
    15481 cgcgttcgct ggcgcactgc caccaaccac tgcgcggtgt catcacgacc agtggcgcaa
    15541 tgtccggctg tccccccgct acgcaggcca cgacgttgaa cgcctccgcc gcctctcgct
    15601 cggcatgggc aacaacgcgc gccggtgtgc cgattgctgc ccgctcggtc agccagccgc
    15661 cttcctcgct gctgacctgc aacaccggcg ggccgaaggg ctcggtaaag gtccgggcgt
    15721 tgctcggcgc tagcccgggg cttctgccgc gggtgataga aacgatcgcc tggtgttgcg
    15781 ccgaccggcg ggcatcgccc agcggcccgg ccgcggcagc gttgggcggt ccctccgtca
    15841 atccgatctc ggtagaggag ccaagcgggc caaaacttcc cgagaccccc tccggcgggg
    15901 tgaagccgcc gtcgaacagc ggcaaccctt cgatgcggcg gccttcgacc tcgacgtagg
    15961 cggatcgcgg cacgacgcga ctcaagctga agggttctag ctcggccact gcaccgcact
    16021 tccggaccct ttcggcgagc catgcggcag agcgccgatc gacctcggtt ccggtacggt
    16081 gccagccctg atcgtcgaac tcgcggacta tgcgatcgat gcgttcttcc tgttcagtca
    16141 tgctgtccct ctttagttgc gatccgttcg gtggcggtgg aacgcgcgct gcgatccgcg
    16201 ggtagttagg gcttgttctt ggagtaattc gccttgtcaa accacaggta cgggtacgtc
    16261 tcggccgccg gcaccggacc cgtcaccgac gagcgtgcgg ttcccgcgtt gcccacccac
    16321 ggccaggtca ggccgtagcc cgccgcctgg tagaagaccg gcgtcgcgat catcgtttcc
    16381 ggcaggtacc gttgggtatc ctggatcaac tgcgcccgct tcttggggtc cgtctctcgc
    16441 agttgggcgt cgatcatctg gtcgagcttc gggtcttcgc ccggcggctg gcgggtcgcc
    16501 gctccctggg aatggaactg gacctgcagc tggtggccgg cgtcgtcgcc ctgcgcggcg
    16561 ggcgtcaacg agatgccgtc ccaggcgccg gcgtgatcgc cgccgaagtg gatcttcggg
    16621 atgtactccg tctgatagtc gaccggcatc agcttgatgt caaagattcc cgtctgattc
    16681 aacatctgcg cgacgatctg caccgtttgc tggtagacag cgttatagcc gttcatcggg
    16741 taatagaact tcaccgacgg cttccccgtg atgccggcgg ccgcaaccag ctttttggcc
    16801 tcgaccaggt tctcctggaa gtacttggcg ttcggcccaa agtccttgcc gaatggatcg
    16861 agccactcgg gccaaccggc gccgatgtgc gagttgatac gaactgcttt ggggagtccc
    16921 gccgcctcga actttgccga gtcagaaatc gtgtcggcga ccagttgccg gtcgaccatg
    16981 taggagacgg cctgccgaag ccgcaagtcc ttgaacggcc cttgaaagcc gaaatatagc
    17041 agtggagagt ccgtgccgta atcggcctgg tacatcgaga tctgcggcag atcctttttg
    17101 agctgcagga cgtcctcttg cttcggcacc attccccaga tgttcgccgc ccggatctgt
    17161 gctagctgcg tcgcatactc gggcacgatc ggcatgctcc aaccgtccag aaagggccgg
    17221 cctttgacgt agtaatcggg gttgtgcttg aattcgagcc ccaccgacgg tctatagcta
    17281 tcgaggatcc agggaccggc gccgcgggcg tcggtcttgg gatcgaaacc gccatccgcc
    17341 tcgtgcggga taacccagat gttgagcgcc cgagacagcg tggcgaggag cggcacgtat
    17401 gggaaggcga gcttcatgac cagcgtggcg gtgtctggat actcgatcga cgtaatcggc
    17461 gccgccttac ttttgctgtt aaagtagttc gaggcgtacg gcgactttgc cagaaagcgg
    17521 cccacgctga acttcacgtc ctgggcgtcg acggctctgc cgttggtggg ggcacgcctg
    17581 tcccagatga ccccctgccg cagcttcatg gtgagctgca acccgtcagg gctgaactcc
    17641 cagctctccg ccagatcgcc caccggctca tttgtagaga tcccgctgaa gttggtcttt
    17701 ttcttgagca gccgcaggta ggagaagttt gcgacggtga tcgtctggac cgacccggcg
    17761 gcgtgcgggt ccattccctc gatgtcctgc tcggcgtatc cctggtagat gcctcctggc
    17821 acagcgctgg ccgaagtatc cccaggcggc gccaggagac ggttactccc gagtgccggg
    17881 ctcgcgtttt gcttcgcccc ggaggtgcct gtgcctgaga ggctaacggc tttgtggttc
    17941 gtggagccgc tgcaagcggt aataccagcg gccgccagcg agaatccacc ggcgccgatg
    18001 agcgcgcggc ggcgcgtcag ccgcccggcg cgcagatgat tccaatatcc cgggtccgat
    18061 gccatcgttc ttctgccctt tctgtcagcg gtagagcggt cgtctatggc ggacgctctg
    18121 ttagtcaccc gccataatac ggcagaacgt ggcatatgct cggcttgtca gcaattgcca
    18181 gactgtttca atcggtgttg agaggcccca acgcaccgag ttgagtagca gcgatcagtc
    18241 tactattgac gatggtacta tgtacgcgtt tactaaagca gatgggggac tcgatggcag
    18301 ccggtatcac gctagtatcc taccatgcgt ggtcgtacga ctggactagg tggtctggcc
    18361 cgctgaggcg tggccatgaa tcaggccgca ccctgaccgt gaagggaggg ccccatgtcc
    18421 gatgaattga aactgaccgt ctttcggtca gcccggcccg gcatccgcaa ggttctgggt
    18481 gacctcgaag ccgacgtgat ggaggttatc tggctgaagc cggccggcga gggcacgacg
    18541 gtgcgcgagg tgttcgaggc gctctacgac cgccggcatc tcgcatacac ctcggtgatg
    18601 aatacgatga cccgcctggc caaaaagaac gtcctgaaag cagccaaaga cggccaagcc
    18661 tacgtgtatt ttccgacgct cacgcaggac gagttcgtct cgcgcttcgt gcgacgtatc
    18721 ctcgaagatc tgtttgtcgc cttctccggc ccgaccgccg ccggtatccg cgcgttgagc
    18781 aatccgctgg ccgcaaagcg agcggaggag ttcgtggcgg atattgcccg ccgacgttcc
    18841 accgaggagc ccggctaaat gcatcccttg ctcggtatcg gcagtctcgt aggcgttgtc
    18901 ttggtcggcg tcctgctcct ttctagcctg cgccgcgtgc gaagctggtc gcggcggcgc
    18961 gacctcgaat tcattttgct cgtcatgcca atggtcagcc tggcgctgag cgtcgcgggg
    19021 ctccgctttg gggcaggctc gctgtgcttc cttggcccgc tgccggtgga ctaccgcgtc
    19081 aatatcgcgc tggccctctt tatggggctc atggccgtgg ccgggctcgc ggggggcgtc
    19141 ctgcgtctgg tgctgttgca gcggactttc gccgcccgcg gacaggcagc gcaaccttgg
    19201 ctggctgcag agataacacg gcttgcgggt ccgctcggca tcagccagcc cgctgtccgt
    19261 atcgtggtgc acgaccgtcc gctggcgctg acgtacggta cacgccggcc gacgctcttg
    19321 ctttcaacct ggatgctggc gcagctcgac aaacaggagc tcagcgccgt cctcgcgcac
    19381 gagctgggcc atgtcgtcca gcgtgactgc gcgatggtct ggctcgcaac cgtgctgcgt
    19441 gacgcgttct ggtatttgcc aaccagccgg atagcctatc ggcaattgca acaggacaag
    19501 gagccagcct gcgacgattt tgccaatgat atcaccgacc gaccgctggc actggcgagt
    19561 gcgctggcca aagtttggcg gcacgcggcg ctggcacccg cggttggtat ggctcaatcg
    19621 ctcacgggcg tggacacctc gattgaaacc cggattaccc ggctgctcgt agacgcgacg
    19681 ccgggcgtcg cgtctatccg gcaccatcgg cgaaccctgg ccgcgggcgg ggtggcgctg
    19741 gccgcgctcg tgaccctggc ggtgaccaat gccgtcgtgc tcttagcccc gatgggctgc
    19801 gggtcgatgg ctcccctggg aaagctcttg tagtcatgcg tgctgtacca ccggatgtcg
    19861 agacgccaca aatgcagtca cagggtgcca gacggcggct tccccgcgtg gcgctggcca
    19921 tcaccacact cgttctggtg gttattgcta tctggtggct gaacgctgga accccactgc
    19981 cgggacgaag ccgcacctcg gacgcgaccg gccaggcggt ctcggacggc gccggcggct
    20041 acgtggcctt tgccgtgccg gcgaaccatt caaccctcgc cagcgtaccg gcgccaaaga
    20101 tcggcgctct ggcgccagat tttaccctgc ccgggctcga cggaacgccg gttcgcctca
    20161 gtgacctgcg cggcaagacg gtcctcatca acttctgggc cacctggtgt ggaccttgtc
    20221 gtaaagagtt tcccgagctt gttaaactgg tccagcagca aggcgaccgc gggctggtcg
    20281 ttttggcggt cgacgtgagc gagagccgcg acgacgtggc ccgctttgcc caggaatttg
    20341 gcgcgacctt tccgattgtg ctggacagcg acagcagcgt ggtgcagagc tatcgactca
    20401 ttgggttgcc gacgtcgctg ttcgtcgatc gcgacggcat cctgcgcgct cagcagctgg
    20461 gcccgctgtc cgaagcgagt ctgaccgcga agttgggcca ggccggcttg cacctgtcca
    20521 tagggcgata aggagaatcg atggagcttg ccggcgttaa catcctggtc gcgttcctcg
    20581 cggggctcgt atcctgcata tcgccgtgcg tgctgccgct tgcgcccgtc tacgtcggca
    20641 gcctggccgg caatatggcg gcagcggggt ctgttgaccg ccgtgccccg gtgatgcatg
    20701 cactcgtgtt catggccggc tttggcctgc tcttcattgc ccttggtctt tcggttggct
    20761 tggtgggcta cgccctgcgg gatcaacagc cgctcctgca gaagctggga ggtatcgccc
    20821 ttatcgtcat gggcctgcat atgagccgga tcattgaaat cccggcgctc tatcgcggca
    20881 tcggcctcaa ctggggtacc ggcggcacac ctggctacct gcgttcgttc ctggccggca
    20941 tctctatctc tgccggctgg cttccctgca tcggcccgac actgggcacc atcctgacgt
    21001 tggcgctcac ctcgggtgcc gtcgtgcaaa gcggcatcct gctccttgtc tacgcgctgg
    21061 gcctggcggt gccgttcgtc ggtatcggcc tcaccctctc acggatgccg ccggccgtgc
    21121 ggttcctgca gcgccaccac gacctcatcg gtctggtcgc cgggcttcta ctcatcgcca
    21181 tgggcatcgt cgtcttcacc ggaaccatgg cccgcttgaa tgccgcgttc ggcttcactt
    21241 cgagtggatt gggggcaaag ttatgaatcg attgaagacg gtggttcctg aaaaggaacg
    21301 ggtttgaaac atccgattcg cgtggtgagc atcctcggct tcgtgctgtt gctgccgctc
    21361 ctgatcagcg cctgcggtac gaacaagggg tcatccggcc cagccgtgag cgagttgaac
    21421 aaagttacct cgatcaaacg cggtgagccg gcactcgtct tcgtctacac cgacggctga
    21481 gtgccgtgcg cacaaagcgc gcccgtggtc gatgggctcg agcaggacta cgccggcaag
    21541 ttgcaggtaa tccgcctcaa tttcaacgac accaaaaacg ccaaggctct tcagacgctc
    21601 aacgtctcca gccacccgac ggtggtgctg atagaccgca aggggacgcc gcaggggtcg
    21661 ctcgccggga ttgcgacaga cgccaagctc aggcccaata tcgacgcgtt gctatcccaa
    21721 tagggggagt cgtgtgcggc tccacgacta tccgcaggac actcgtcgga cgggaccagc
    21781 ggtcgttctc gtgccacggt ggctgcggga gcgccgcgcg ggcttgaagg aggacaccat
    21841 gccctgcacc gcggtcgggc gaccacagcg tggccatcgc ctgttcgccg ccctgtacga
    21901 ccgggtcact gcggcgtccg agcgtaagtt cgtggtacgg caccgagccc aactcctcgg
    21961 gttgctagac gggaaggtgc tggaaattgg tgtcggcacc ggggccaact ttccctacta
    22021 tccgccggct gcggaggtcg tcggtattga gcccgacccc ttcatgctcc ggcgggcgga
    22081 ggtcaggctc gcccggtccg gccggccgaa catcacgctg caatcaggca gtgccgaacg
    22141 gctgctcttc gccgatcaga gcttcgacca tgtcgttagc acgctcgtgc tctgcaccgt
    22201 ggcggacccg ctgcaggccc tcgccgaaat ccggcgggtg ctcaagtcgg gcggagcgct
    22261 gcacattatt gagcatgtgc gcgctgaagg gtggctgggt cacggccagg acctgctgcg
    22321 cccggcctgg caattcgtcg gtgcgggctg cgtcctcaat cgccgtaccg ccgagctcat
    22381 tcaagcggcc ggcttccgta tcgagacgct ggtgccgtac cggatggcgt tcgggatacc
    22441 catgctggtc gggatggccc gcccaaccta acgagtgtct gctcgtccca gccagcgagt
    22501 gccatgcgac aacgtctcca gacgctcaac gactccagcc acccgacggt ggtgctgata
    22561 gaccgcaagg ggacgtcgca ggagccgctc gccgggattg caacaggcgc caaactcagg
    22621 cccaaaatcg acgcgctgct atcccaatag ggggagcgcg gtcaccagtt gctcgttgcg
    22681 gcaccggacg cttgcgtcca ggcgattcga tcggcggtcc tgctgcctta cggctgagtg
    22741 ccgaccacgg tagatgtgcc tgcagatgcc gccaccgaac tgatgcggac gagggcgttg
    22801 ccatcgtcag cagcctccgc aatccacacc gaaccgtcgc ctgctggggc cggccaccaa
    22861 gggcctgcag cggttctgaa ggtgcggaat tgctgtgtag tggaatcgaa tgcatagacg
    22921 gtatttccag tcgtccccac ccagataacg ccgttggcgt ccaacgtcac tccgtaggca
    22981 tccccacccg gcaacgctag gagcgaaacc tggccgttgt gcgggtccag gcgacccagc
    23041 tttccgcctt ctgcgtttgc gccgaaccag acggttccgt cggcagccac gtcgatcccg
    23101 gtcgttcgct tggtgcccgg cacgctggta atgtcgatac ggccgctggc gggatccagc
    23161 acaccgatcg aggcggaggc gatgtcggtg aaccacaccc gaccatccgg tgctatgtgc
    23221 agccaataag gcaggaactt cgcgtccggg acgggatact cggcaaaagt acgggcagcc
    23281 gggtcgtaca ccccgacctt gtgcatggcg agttctgtga accagacgcg gccgtggctg
    23341 tccagcgcaa ttcccgaggg gttcgaattc ggggtgggta tgggaatctc tcgatagtca
    23401 ccggtaaccg cgtcgaaccg gccgagcgca ctaacgccgc tctgggcgag ccagactaca
    23461 ttcgcggcgt cgacaacgac gcccatggat ccgatggacc ggccaggtgg ataggccatt
    23521 tcagtgtacg tgttggtgga cgggtcgagg acggcgagct tattcgccgt catctcgcca
    23581 atccagatcc gcccgtcgga accgatcgcc ggaatcatca acccgctacc ggaagttggc
    23641 accttccagc ttgagtaatc agccgagcgg accggtgcga cagtagttgt gaccggacga
    23701 ccggtgcgtc cagcggccgc cgcccctggg ccggttgaag gcgacgccac gggtccggcg
    23761 gctgcttgca gttcgccgcc ggcgcctgtg cgtgaccccg acgaaggtaa ggaaaccacg
    23821 aaaacggcca ccgctacgag agcagtcgtg gacgcggcca gcgccacggt ccgcggcgga
    23881 agcgcgggaa acccaaagcg ccagcgcagg acggcaagca ggaccgccgt cgcaagcagc
    23941 acagcggccg cgccctgggc cggtgtaatt ccatgcgccg ggtatgcggt cgcgtcgatg
    24001 gctgcggcgc cggagctgcc gggaaccgtg accctgaatg cgccttcgac gtcatcatgc
    24061 tgtgcgcgtc gcacattgac gcggatctcc cagcggccgc caacgcccag gtttgctgcg
    24121 ctggcggcga agagtccggg ggatccggcc accgggtcga gcgtgacggt tgagccgccg
    24181 gtctgttcct tcgcggactg gaagcgaagc tgaacccgtt gtgccacatc cacaccggga
    24241 ctcgcgagct gcacgcggaa ctcgttgtcg ccgatccggt tcggattgac gcccagcgat
    24301 attcgcaggt ctcccgcgga ggtctcgcgg ctgtatacgg acgaggcggc agccgccgcc
    24361 ggctgccggc cgtttgcaga tgccaccgga ttcctcgccg gaacgaggaa cacgagaacg
    24421 gcggcggcgg cgacaactat tgcgccggcg atactttcga gcacgatcac acgcgcgaga
    24481 cggcgccctt gctgctcggt ctgatcgtcg ttgcggtggg agaggcgttc gaagcgcgcc
    24541 gcgatgcgga ccgagttcag cagcccaatt ccgagcagcg gcaacacgaa agcgagcttg
    24601 acgagcaatg tacggccata cgccgtgtcc ttcaacgcgg ccggcgaagc gatctgtacg
    24661 agggcattga aggtgccgct aagcaagacg atggcaacgc tgcaggccgc caggatcgaa
    24721 aaccggcgca aagccgagga ttgcagccgg cgtcgaatcg ggttcgatag gccgggtccc
    24781 atgctcagaa ccgccggcaa ctgcactaaa cagccgagcc agaacgcgac gccggtcagg
    24841 tgtaggaagt cactgaggac ggaccagtca gagccgattc cggccgccgc gtgtgaggtg
    24901 agcgacatgg tgagcagccc gcccaggccg gtgaccaggc cggcccacag cagcgccgcc
    24961 gtcagccggt cgttggcacg acgggcggtt ctcgtcaccc accaaaccag gcccccggcg
    25021 atcagcgcca gcgcctcgcg ggccagccag taggtgccgg ttctgccgct caggaggtcg
    25081 cgcagggcgc cgagcgagcc gatctgctct gcttgccgca gcagaacggc cgcgttgatg
    25141 atcgcgacga gaatcagcgc ggcagggacg accgcgccga gcagccaaag tgtaaattgc
    25201 ctggctttga ggcggtcgga gcctgccaga gctccggctg ccggaaacag tatcagcgcc
    25261 gcaaacccga atccgccggt gatcccgaac aggccgaagt aaagtagcca gcggaggacc
    25321 gcatcgggag gttggagcgc ggacgacccg ccgccctcag tgggcgggcg ggctatgccg
    25381 gccggcgccg tcccgtcggc attgagcacc gtaaacggga acgtaccttg ccagcggtgg
    25441 gcgtcgacat cagaaacagt cacccaattg acgtaataga atcccggacc gagcggctca
    25501 acgctcacgg tcacttccgt tgggtcgtcc gtgaatcgaa cgtcacgctt atcgtgccga
    25561 gcgccggaag aatcgaaaac ctggatcgtg ctgaatgaat ggttggccgg ctcgctgaag
    25621 aagagatcga tgcgctgcgg agacgcggtc agccgcgcgt tctgcggcgg atcggagcgc
    25681 accaagacag cgtgggccag cgctgccgaa ggatgacctc cgagcactag cagggtgcca
    25741 gccacgagga ataccacgca gcgagatgcc caccacggca ttcgtagccg cgcccagcgc
    25801 gccgagtctc catacctgct gctggtcacc acgttacctc ggtgaatgcg ggaaggcgtg
    25861 ttgccggctc agcatctcat cattcggcgc atcatatctt ccatcggccc ggctgaatcg
    25921 cgcatgctgg acacgccgta ccaactctgg aggtagccct ccatctcggt gatcccgacc
    25981 gcctgctttt agactctacc tatcggtctg ccaggatgag caccggccct acggcaaagc
    26041 ctaccatgaa cagtagtact accaacatat gtatcttgtc atgcgggccg ggagccgttg
    26101 ttctacgatc gacagtcgta cgatggccgt gtatactttg ctttaacacc gcgaccgaac
    26161 gctgccaagt gcggttgtaa cattgggtgg aggtatccga cgtgaacttg aaaagctgtc
    26221 tgaactggaa agtcatcggt ggacttgcgg tggtcggagc cggcatctgg attgtgcagc
    26281 ccgggctgat cagccgcgcg ctaccgctac tgctcattgc tgcatgcccg ctctcgatga
    26341 tgttcatgat gggcggcatg ggcaaaatga tggggggcag caagcaggac ggtgccacgg
    26401 gcgcctatac ctgcccgatg catcgtgacg tacagagcga ccaaccgggt acgtgcccca
    26461 agtgcgggat gagcctggtt ccgaccgcgg cacccacacg gcaggcgcag gcgacgccga
    26521 tgggcggcac gagcatcgct ccagcacaag gggcgaacgc ggcacagctc cgagcgcagc
    26581 ttcaggacct cacggcgcag cagcaagtgc tggcgcagcg gctcgaggag ctggacgctg
    26641 ccacagagac ggccgtaccg agcaaggtgc tccgggacgc ggaagcggta gccggagcgg
    26701 ccgatgcgga caatcgccac ccgtgaacgg caccgagatc gggatcgatc caacgatggc
    26761 acagcttggg ccgttcgcgc tccgttgcta caggccggga gcgtgacaca gcaagaggtc
    26821 tgggatctct ggtatccgaa cgccgctgct caggggctct cctttgcccg cggccgcctg
    26881 gatagcaccg acgtactgct cgtccacgct gccccggata gcctgcgcgt tcaggtttct
    26941 gacgatgccg gaaagcaact cgcctttggt gaccagctca ggcgcactgc cgaccgaccg
    27001 atgacgcgcc tgcgccgcgt gggcagcacg atcacgcgcg aggatctgtg gccggaggag
    27061 caggatatcg gtcgaccggt catcgtaccc ggcggggagg tcggtatcat caggtcctgg
    27121 tggaacgccg ccgacgggag tgaatggcgc tggcaggtcg agttctataa ccgtcggtag
    27181 ctcaccagcc gcagcgggtg tgggatcatc gtgaagcgcc tgctgaactt caacgacagc
    27241 cgctcgtcgg tattgcgaca gacgccaagc tccggaccaa actcgacgcg ttgctatctc
    27301 ggtagggaga ccatttatgg cccagccgct ttttgttcac tgcaccggcc gcggcgggcc
    27361 ggcagtcgtt cttttgcatg gtatgggggc cagcggtcgc ttctgacggc tggtggctga
    27421 acagcttgcg gacgaggcgc gcatcttttg cccggacctg ctcggatttg gtcgcgcgcc
    27481 gcgcccgagt gcggcctaca gcgttgccga ccatctggcc gcgcttgacg cctgtcggga
    27541 tagcctagcg ctgggttccg agccggtcat cctcgccggc caatcggcgg gttcggtact
    27601 cgccctcgct gggctgccgc caggccggag cggtttgccg gcctcgggtt gctggcgctg
    27661 cctgcttatt cgtcggtcga agatgcccgg cgacacatcg gttccatttc cgcgctggca
    27721 tcggcgaccg tggaccgccc cgacctcgga gggctcatct gtgagtggcc tgatgtgcgt
    27781 cgggcgcccc ctttggcagc gcctgatgcc gctcgtgatg cgccagttcc cggcagatat
    27841 ggcgcgagac atggtcctgc aagaccgggt gtcctaccgc aacacctgca acaatgttct
    27901 cgtggaccat cgctgcgcgc catcggcagc gcaggtggcg aaagccaaaa taccggtgca
    27961 gatgctccac ggcagcaagg acaaaaccgc gccgcttgcc catgtgcagc agctcgccgg
    28021 acagttcggc tggccgctca ccgtcctgga aggccgcggc caccggctcc tggtggaagc
    28081 gccgcatgcc tgcgccggaa ctattcgcac gttgctcgcg gacaacatgg cgatcgccgg
    28141 gcactagccg ggttaccgtc tgcgcaaacc gatcgccagc agaaccatgc cggcgaccaa
    28201 gctgtcgaac gcagcccccg gcgaggggga cggatgtaaa actgccgtta gccgtccgtg
    28261 acgcgcctcg acaccaatcg cccgagaaac gagttgccca gcaccacgct gacgctggcg
    28321 gccatcgcga ccatggccca gaccggctgg acgaggcccg tcaccgccgc cagcaccccg
    28381 atgccgttga atgcgagcgc cagggttacg ttggtcacag tcagctgata gctgcgtcgc
    28441 gacagttccc gtgcctcgac cagcgttccc agccgctcac tgaccagaac gacgtcggcg
    28501 gcctccagcg caatatcggt tccggcaccg atggcgatgc cgacatccgc ttgcatgagc
    28561 gccggcgcat cgttaatccc gtcgccgacc atggcgactt tgttgccctg tgcctgcagt
    28621 cgtcgaactt cggccgcctt ctccgcgggc agcacctcgg ccaaaaccac ctcgatgcca
    28681 accgcctcag caaccgcccg ccctgtgcgc cagttatcgc cggtcaacag caccgacgtg
    28741 atgccgagcg tccgcagccg agcaattgcc gattgggcat ccggcttgag ccggtccgcc
    28801 agggcaaaca cgccggcggc acctccgtcc aatgccgcga gcaccgcagt ctgaccgcgt
    28861 gcctgcacct gctcgaccag ggccgcaatg ggcgtggtat cgataccctc ttcgcttagg
    28921 aaccgctgcg tgccgactac cgcacgctgc ccacggacgc tcgcaatgat gccgcggcca
    28981 ggtcgagctt caaagctggt ggtcggtgtg agcagcaggt tcttcctgcg tgcttcgtcc
    29041 acaatcgcct ggcccagcgg gtgctcggac agtgcctcag cgctggccac aagctgtaag
    29101 agttcctcgg catcgccggc cggggaaaag taggccacga gctctggttt gccttcggtg
    29161 agggtgccgg tcttgtcgaa cacgatggtg cggacgttct taaagacgtg gaacgcctcg
    29221 ccagagcgca tcaagatgcc tcgttcagcg gcttcgccac tggcccggat gatggccagt
    29281 ggtgtcgaca tgcccaacgc acaggggtac cccatgatga gcacgcttaa cgcggcgaag
    29341 ctggcccgcg gccagttggg ctcgccggcg aatgcccaag cacccagcgt ccaaatgagc
    29401 gcccctgcag ccgccgccgc aaacacgacc ggtacgtaga caaggagcac gcggtccacc
    29461 aggcgaagaa tgccgggttt gagtgcccgc gcttcggcca cgtgccgtgc caccgtgcgt
    29521 aagaaggtat cggagcccac gcgcgtgacg cgaatgacga cgctgccggc ctggttgagt
    29581 gcgccgccga taacctcgct gccgggcagc ttatcgacgg ggatggactc gcccgtgacc
    29641 aggctttcgt ccaccgccga ggcgccctcg gtcacgactc catcgatggg tatgcgctcg
    29701 cccggacgga cgcgaacagc atcaccgacc aggagttgct cgacaccaac ctcgacctcg
    29761 ctaccatccg gctggacacg acgggcggtg ggcggctcta acgccaggag ccggcgcacc
    29821 gactgcgagg cacggacgtg gacgagcacg gacgcgtagc caccgatgag atggaagctc
    29881 agtacgaaca cggtggcgcc gaagaagccg ccagcgggca ggcgatggac gaacagaccg
    29941 actacgccac ccagtagccc gccgagcgcc gagctcgtga cgagcacgtc ctgattgagg
    30001 atgccgcgac gaacggactg gtagccgttg cgggcgatga tgaagcgagc cggacctaag
    30061 acaatgaacg ctgccagcac gcccatcgcc agcgagcgat agagattggc gccggcaacg
    30121 acggtgaaca ccatcaaggc ggacgcggcc agcagcaggc caaccgagag gaaggccttt
    30181 cggcgggcca tcgccaggtc gtgctcctct tctgcgaagc gttcggcctg gtcgggctca
    30241 cgcaccgtgt agccgatgtc ctcaagcgcc gcttgaatcg tcagcgggtc cacccgtgct
    30301 gggtcgtact cgaccagcgt ctcctcgtgg gcgatggaga cctgcacatc cttgacacca
    30361 tccaggcgac cgaccgagcg ccggatggag tcggtgcaca acgagcagtg cattccgccg
    30421 actttgagct gcaatttctg cgtggtgtcg gctggtgcgg gcagcccgtc cgggctatcc
    30481 agcgaccgaa cttcggtgcg tgtgctcatg tggtttctcc ttgaagggcg gtctcgaagc
    30541 cgccctgggc aagcttggtg cggatggcgt ctgcctggac ctggtcgggg tcaaaggtga
    30601 cgaccgcctg ctgggtttcg tgactcgctt gtacacgctc gacgcctgtg aggcgccgca
    30661 gcatggtgtt gatacgctgc tcgcagctct cgcagtgaat cgtgtcccca ccggtgaccg
    30721 cgaactgtat cgtctgtgtc atggtttcgt tccctcttta tgggaatggc cggtcgtctc
    30781 gtacccgtgc gttgctgacg tcacggtcaa cgctacggct tccagttgac tggaatgtca
    30841 agagccacgc ggagtacggg cttggccctt gcggcggatc gtgccgatgg gggtgatggc
    30901 gttacacggc gattcgtgtt tcgtggcgag ttcgtggtgt tcaataatcg cacagaccgg
    30961 tgcatcccta tccacgctct tggcggcttc atcgcggagt gccgccagct cctggcgaaa
    31021 cgctgtcagg gcgcggaggt gctggtcgac ggcggcgacc ttctggtcga gcaggcccag
    31081 aacgtgcacg cagggtggct ggccctcacc gcgcagggcg aggatgtggc cgatttcctc
    31141 gagtgtgagg ccaatcgctc gggctttgac gatgaactgc aggcggtcgc ggtcggcggc
    31201 atcgtagagt cggtagccgg cgggcgtgcg gcgcggtcgc ggcaggaggc cgatgtcctc
    31261 gtagtagcgg accgtcttcg gatttagacc caattggccg gcaagctcac cgatgcgcat
    31321 ttcggcgctg agcagctgtt cgctcatgat ggtactccta aggttccact gcacaggaac
    31381 cttcatcagt gtaaaccttc cagggagctg gaaggtcaag gagtgccaag gcaatgagtt
    31441 caatccaccg cgatacaggc ttcacgagca gataccagac ggccgagagc ttctttcgcc
    31501 tccacgcccc gcgcgtccgc gaccatcccg accaaattcg ccggtctcac acggaaaacg
    31561 cccgaggtct atttaccgct tgactctggt gctagctcca aggtttagcg tgcttctgga
    31621 ggcgg
//
LOCUS       DATCPE010000104         4900 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U7219
            k141_258215, whole genome shotgun sequence.
ACCESSION   DATCPE010000104 DATCPE010000000
VERSION     DATCPE010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955033
            Sequence Read Archive: SRR1586279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 4900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 16:49:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 640
            CDSs (total)                      :: 631
            Genes (coding)                    :: 627
            CDSs (with protein)               :: 627
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4900
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_258215"
                     /isolate="SMAG_U7219"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..929)
                     /locus_tag="VIM45_03145"
     CDS             complement(<1..929)
                     /locus_tag="VIM45_03145"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY8820316.1"
                     /translation="MRIGLDCKPEGWAATTIGQVRKEIIMDLGLSEEQEMLKNVARDF
                     LEKEVPEKYVRDMEEDDRGYSPEVWSKMAAQGWQGLLIPEQKGGSGFGFLDLIVLLEE
                     FGRALVPGPFIPTQQATIALLEGGSEAQKSQYLPRIASGEAIATLAITEPSARWDAEG
                     IQLQATKSGDGYTLNGTKLFIQDANVADLMVVAARTGGTDEQGISLFIVDAKSSGIST
                     EVLKTIAADKQCEVKFENVKVPAANLIGQDGKGWEILQKVQLKATVMECAYLVGLAQM
                     DFEISVNYAKERVQFGRPIGSFQAIQHKCADMVT"
     gene            complement(1038..2660)
                     /gene="groL"
                     /locus_tag="VIM45_03150"
     CDS             complement(1038..2660)
                     /gene="groL"
                     /locus_tag="VIM45_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871329.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEY8820317.1"
                     /translation="MAKQLLFDEDARRALKNGVDALADAVKITLGPKGRNVVLDKKFG
                     APTITNDGVTIAKEIELQNPFENMGAQLAKEVATKTNDVAGDGTTTACVLAQAIVREG
                     MRNVAAGANPMVLRRGIDQGVRAVVASIRDQARPVSGKEQIAQIASISAGDTEIGAMI
                     AEVMEKVGKDGVITVEESRGLKYETEYVEGLQIDRGYVSPYFVTDTQRMEAIIEDPYI
                     LITEKKLSAVADILPPLEKVLSVSKNVVIIADDCDGEGLATLVVNKLRGTVNVLVVKA
                     PGFGDRRKAMLEDIAILTGGTFITEETGRKLDQVQVEDFGRARRVVASKDDTTIIEGF
                     GSDEAIQDRIKQIKGQVDDTASDFDREKLQERLAKLAGGVAVIKVGAATEVELKEKKA
                     RVEDALSATRAAVEEGVVAGGGVTYLCAQSALDAVENELKGDERTGANLLRKALDEPL
                     RLIAINGGKEGNVVVGNVRKNANGNAAYGYDALLDDYGDMFKKGIIDPAKVTRSALEN
                     AASIASLVLTTESLITDIPEPPAAAAPPPMDY"
     gene            complement(2711..3010)
                     /gene="groES"
                     /locus_tag="VIM45_03155"
     CDS             complement(2711..3010)
                     /gene="groES"
                     /locus_tag="VIM45_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEY8820318.1"
                     /translation="MASKIIPLADRVVVKNIQAEETTASGLVIPDTAKEKPQQGEVVA
                     VGPGRIDDNGKRVPLDVSVGDRILYAKYSGNDVKLDLIEYLVLSEKDILAKIEIS"
     gene            3188..3439
                     /locus_tag="VIM45_03160"
     CDS             3188..3439
                     /locus_tag="VIM45_03160"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY8820319.1"
                     /translation="MSERRGWYGSRQPKWDATGLLALRRRMGLTQQQLADELGVRQQT
                     VSEWETGVYRPRGASARLLGIVAERAGFVYDESEGEAKQ"
     gene            3436..4740
                     /locus_tag="VIM45_03165"
     CDS             3436..4740
                     /locus_tag="VIM45_03165"
                     /inference="COORDINATES: protein motif:HMM:NF022466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2851 family protein"
                     /protein_id="HEY8820320.1"
                     /translation="MIHFGAVCERPRRYRVRPPFRESELSLLWEAQQFPPEAIVTDSG
                     QDVTVLYRGRRGAGPGPDFRDAAIALAGGRLLKGDIELHVRSSDFQRHGHHLDRGYQR
                     LVLHVVFEHDGGPTLVLDGREVPIIALGRWVQRRAGELRATLADPGSYREPCHTAVAR
                     LGEAAAVGELSEIGARRLREKAARLLLLVESAGPTESLYRATARALGLTRNCAQMEAV
                     STALPLAELNAIAADAADRELLIEAALLGTGGLLNGQTAMWADAEAVREQRLLTAWQA
                     LGGQPLRLEWDATPNRPGCGPRERLAGLAALVGRDGPPLNASKAEWLELLSRGPRATM
                     AVLQTGSNIGRDRAIEIAVNAVLPWLLAAYKGDAELEASAVALYQELPGPVAYGATRL
                     LTSALVDAEGRPLVRGAAAVQGALQMTRDWCTRGGCGRCPLS"
BASE COUNT          783 a         1588 c         1558 g          971 t
ORIGIN      
        1 gtcaccatgt cggcgcactt atgctggatc gcctggaacg agccgatcgg gcgcccgaac
       61 tgcacgcgct ccttggcgta gttcacgctg atctcgaagt ccatttgcgc caggccgacg
      121 aggtaggcgc attccatgac cgtcgctttg agctggacct tctgcaggat ctcccagccc
      181 ttaccgtcct ggccgatgag gttggcagcc ggtaccttga cgttttcgaa cttgacctcg
      241 cactgcttgt cggcggcgat ggtcttgagc acttccgttg aaatgcccga actcttggcg
      301 tcgacgatga agaggctgat cccctgctcg tccgtgccgc cggtgcgcgc cgccacgacc
      361 atcaggtcgg cgacgttcgc gtcctggatg aagagcttgg tgccgttcaa cgtgtagccg
      421 tcaccgctct tcgtcgcctg caactggatg ccctcagcgt cccagcgtgc ggaaggctcg
      481 gtgattgcca gcgtcgcgat cgcttcgccc gaagcgatgc gcggcaggta ctgcgacttc
      541 tgcgcctcgc tgccgccttc gagcagcgcg atcgtcgcct gctgggtcgg gatgaacgga
      601 cccggtacga gggcgcgtcc gaactcttcc agcaggacga tgaggtcgag gaagccgaag
      661 ccggagccgc ccttctgttc cgggatgagc agcccttgcc agccctgtgc ggccatcttg
      721 ctccaaactt cggggctgta gccgcggtcg tcctcttcca tgtcgcgcac gtacttctcc
      781 ggcacttcct tttcgaggaa gtcgcgcgcc acgttcttaa gcatttcctg ctcttccgaa
      841 agtcccagat ccataattat ttcctttcgc acctgcccga tggtcgtggc cgcccagccc
      901 tctggtttgc agtcgaggcc gatgcgcatg gtacaacgat gcgtgggcag tcggcaatcg
      961 ggcgcgcagc ccggcagtcg tgaggtggcc cggcgcaacg aaaagggccg gggcattcgg
     1021 cccggccctt ttggcaatta gtagtccatc ggtggaggcg ccgccgccgc cggcggctcc
     1081 ggaatatcgg tgataagcga ctctgtggtc agaacaaggc tggcgatcga cgccgcattc
     1141 tcgagcgccg accgggtcac tttcgcgggg tcgatgatgc ccttcttgaa catgtcgccg
     1201 tagtcgtcca gcagcgcatc gtaaccgtac gcagcgttgc cgttggcgtt cttgcgaacg
     1261 ttgccgacaa cgacgttgcc ttccttgcca ccatttatcg cgatcagccg cagcggctcg
     1321 tcgagcgcct tacgcagcag gttggcaccc gtgcgctcgt cgcccttcag ctcgttctcg
     1381 acggcgtcga gcgcggattg ggcgcagagg taggttacgc cgcccccggc gacgacccct
     1441 tcttcgactg cggcgcgggt cgcggagagc gcgtcttcaa cgcgggcctt cttctctttg
     1501 agctcgacct cggtcgcggc ccctaccttg atgacggcga cgccgccggc cagctttgcc
     1561 agtcgttctt gcagtttctc gcggtcgaag tcagaggccg tgtcgtcgac ctgacccttg
     1621 atctgcttga tgcgatcctg gatcgcctca tcggagccga agccctcaat gatggtcgtg
     1681 tcgtctttgc tggcgacaac gcggcgggcg cggccgaaat cctcgacctg cacctggtcc
     1741 agtttgcggc ccgtttcctc ggtaatgaac gtgccgccgg taaggatggc gatatcttcg
     1801 agcatcgcct tgcggcgatc gccgaagccc ggcgccttga cgaccaggac gttgaccgtc
     1861 ccgcgcagct tgttgacgac gagcgtcgcc agtccctcgc cgtcgcaatc atcggcgatg
     1921 atgacgacgt tcttactgac cgacaggacc ttctcgagcg gcggcaggat gtccgccacg
     1981 gccgagagtt tcttctccgt gatcaggatg taaggatcct cgattatcgc ctccatgcgc
     2041 tgggtgtcgg tgacgaagta cggagagacg tagccgcggt cgatttgcag cccttcgacg
     2101 tactcggtct cgtacttgag gccgcgggac tcctcgacgg tgatcacgcc gtctttgccg
     2161 accttctcca tgacctcggc aatcatcgcg ccgatctcag tgtcgcccgc ggagatcgac
     2221 gcgatctggg caatctgctc cttgccgctg accggacggg cctgatcccg gatagacgcc
     2281 accacggcgc ggacgccttg gtcgatgccg cggcggagaa ccatcgggtt ggcgccggcc
     2341 gcgacgttgc gcataccttc gcgcacgatc gcctgtgcca gcacgcacgc ggtggtcgtg
     2401 ccatcgcccg cgacgtcgtt ggtcttggtt gcgacctctt tagcaagctg cgcgcccatg
     2461 ttctcaaacg ggttttgcag ctcgatctct ttagcgatcg tgacgccgtc gttggtaatt
     2521 gtcggcgctc cgaacttttt atcgagcact acgttgcggc ccttggggcc gagcgtgatc
     2581 tttacggcgt ccgcaagcgc gtccacgccg tttttaagtg cacgacgagc atcctcgtcg
     2641 aacaatagtt gtttggccat aaccggttcc tttcggcgaa cagtagccta ttaacgagcg
     2701 agcacgccga tcaggagatc tcgatctttg cgagaatgtc cttttcggac agcacgaggt
     2761 attcgatcag atcgagcttt acatcgttgc ccgagtactt ggcgtagaga atgcggtcgc
     2821 cgacgctcac gtccagcggc acccgcttac cgttatcgtc tatgcggccc gggccaacgg
     2881 cgacgacttc gccctgctgg ggcttctctt tcgccgtatc cggaatcacc aagccgctgg
     2941 ccgtcgtctc ttcggcctga atgttcttca ccactacccg gtcggccagg ggaatgatct
     3001 tcgatgccac cgttgtgacc ctcctgcttc caaataaata gtctcggcac tttagcactc
     3061 gcacaatgag agtgctaaca agggcatcgt acgcaactgc tccgagacaa gcaagacccc
     3121 cactatcgga ttgatctcaa acccggcgat ccttgtcagt ttacacggaa ccgtgtagac
     3181 tgcaggtatg tcggagcgac gcggatggta tggatcgcgg caaccaaaat gggacgccac
     3241 gggactgctt gccctgcgcc gccgcatggg gctgactcag cagcagctgg cggacgagct
     3301 cggcgtacgc caacagacgg taagcgaatg ggagaccggg gtctaccggc cgcgcggcgc
     3361 ttccgcccgc ctgttgggca tcgtcgccga gcgcgccggt ttcgtgtatg acgagtccga
     3421 aggtgaagcg aagcaatgat ccactttggc gccgtttgtg agcgacctcg gcgctaccgg
     3481 gtacgcccgc cctttcgcga aagcgagctc tccctgctat gggaggccca gcagtttccc
     3541 cctgaggcga tcgtgaccga ctccgggcag gacgtcaccg tcttgtatcg agggcgccgg
     3601 ggcgccggcc ccggccccga tttccgtgac gccgccatcg cccttgcggg cggtcggctg
     3661 ctcaaggggg atatcgagct gcacgtccgc tcctccgact tccagcggca cggccaccac
     3721 ctcgaccgcg gctaccagcg gctggtgtta cacgtcgtct tcgagcacga cggcggcccc
     3781 accctggtgc tcgatggtcg cgaagtgccg attatcgcgc tcgggcgctg ggtgcagcgg
     3841 cgggccggcg agctccgcgc gacactcgcc gacccgggca gttaccggga gccgtgccac
     3901 accgcggtcg cgcgattagg agaggccgcg gcggtcggag agctcagcga gatcggcgcc
     3961 cgccggctgc gcgagaaggc cgcccggctg ttgctgctag tcgaaagcgc cgggccgacc
     4021 gagtccctgt atcgcgctac ggcgcgggcg ctcggcctga cgcggaactg tgcccagatg
     4081 gaggccgtat cgaccgcctt gccgctggcc gagctcaacg cgatcgccgc ggacgcggct
     4141 gaccgcgagc ttctgatcga agcggcgctg ctgggcacgg gcggtctgct aaacggtcaa
     4201 acggcgatgt gggcagatgc cgaagccgtg cgggagcaac ggctactgac ggcatggcag
     4261 gcgctgggcg ggcagccact gcgcctcgag tgggatgcca cacccaaccg cccgggatgc
     4321 ggcccgcgcg aacggctggc ggggctcgcg gcgctggtgg ggcgcgatgg ccctcccctc
     4381 aatgccagca aagctgaatg gctcgaatta ctctcccgcg gaccgcgggc gacgatggcc
     4441 gtcctgcaga ctgggagcaa catcggccgc gaccgggcaa tcgaaatcgc ggtcaacgcg
     4501 gtgttgccgt ggctgcttgc cgcctacaaa ggcgacgctg aacttgaagc gagtgccgta
     4561 gctctatacc aggagctacc cggcccagtg gcctacggtg cgacccgcct gctgacgtcg
     4621 gccctcgtcg atgccgaggg gaggccgctg gtacgcgggg ccgcggcggt gcaaggagcc
     4681 ctgcagatga cgcgcgattg gtgtacgcgg ggcggctgcg gtcggtgtcc gttgtcgtga
     4741 acgggccacg gcctcaggca acggtgccgg ctgcctcggt ttccggggcg tcgagttgga
     4801 cgagccagcc gcgatatttg gctagcttgg cttcatattc ctgctgcatc gataagagtt
     4861 gctcaacctt ctgccggatc aactcgtact gtttttccgt
//
LOCUS       DATCPE010000105         5626 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U7219
            k141_161444, whole genome shotgun sequence.
ACCESSION   DATCPE010000105 DATCPE010000000
VERSION     DATCPE010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955033
            Sequence Read Archive: SRR1586279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 5626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 16:49:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 640
            CDSs (total)                      :: 631
            Genes (coding)                    :: 627
            CDSs (with protein)               :: 627
            Genes (RNA)                       :: 9
            tRNAs                             :: 8
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5626
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161444"
                     /isolate="SMAG_U7219"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..891)
                     /locus_tag="VIM45_03170"
     CDS             complement(<1..891)
                     /locus_tag="VIM45_03170"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEY8820321.1"
                     /translation="MTTQSRSFRKLGPGVLQATDEAGAPRGAFGRFTAATRRRFIGSP
                     LATSQLAHERLTKVKALAVFSSDALSSVAYATEQIVVVLVVAGTGALGNVVPISVGIA
                     VLLALVAISYRQTIHAYPNGGGSYIVAHTELGVWPGLIAAGSLMVDYILTVAVSISSG
                     VQAVTSAVEGLHPFTVEIALAALAFMVVINLRGVKESGSIFAAPTYVFIFSILLMIVV
                     AFVKILLGHGGGIPQEQHALQSTGASIGIFLVLRAFASGCTAMTGVEAISNGVPAFQK
                     PESDNARSTLTWMAIILGIMF"
     gene            complement(1212..1688)
                     /locus_tag="VIM45_03175"
     CDS             complement(1212..1688)
                     /locus_tag="VIM45_03175"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEY8820322.1"
                     /translation="MAERVPPEASALLNTRNILVPCDGSDICFSALALACDVAHRNAG
                     RVYAVHVIVVRRSLPLDAEMVAETRRGNDILTRAERIGAAIGQGVETELLQARDAGSS
                     IVDEASNLGVDAIFLGLELKERIAEPTVGAKIDHLLQQAQCQVWIFRDTLADEDRV"
     gene            1759..3036
                     /locus_tag="VIM45_03180"
     CDS             1759..3036
                     /locus_tag="VIM45_03180"
                     /inference="COORDINATES: protein motif:HMM:NF005560.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY8820323.1"
                     /translation="MAEIGIIGGGALGLGAGLRLAQAGRRVVIIEREPELGGLAAGFR
                     PGEDSKASLEKFYHHIFKTDRTIIRTIAELGLEEKLVWGQPVSSLLRGGRIYPMSVSG
                     VLRFGAIPFVDRLRFGATVAGLKLVPNERRFAGQTATEWTRRWAGRRAYDGFIEPQLA
                     GKFGNRARDISMGWLWSRFHERTIRLGYLRGGFQQLYDAIGERIRSLGGEILLGASAS
                     AIRSRDGGVQVDTPHGTHSFERLLVTAPQRVFERLAEGLPPDYATSHPGPEFYSAQVL
                     ILALDRPLTDKYWISVGDPGYPFLVLVEHTNFMPASDYGGRHLVYLGNYLPDDSPLLR
                     KSEAEVLAEFLPALKRFNPDFEASWLRDHWLFRAPYAQPIVTTNYVSSLPPIETPLPA
                     VLLATMAHVYPQDRGQNYSLRLGEQMAGLLLAG"
     gene            3125..3340
                     /locus_tag="VIM45_03185"
     CDS             3125..3340
                     /locus_tag="VIM45_03185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8820324.1"
                     /translation="MPLNKFFVEYDEPAGAVGLIDEQVGPLGYSYEYAAAYRRAKQLA
                     KNGATRVRVVRRNRGLPEEVISEVVAG"
     gene            3459..3815
                     /locus_tag="VIM45_03190"
     CDS             3459..3815
                     /locus_tag="VIM45_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012660457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HEY8820325.1"
                     /translation="MAAIQRIANETRATELGGRIHVADNPWTRFVGLIGRRGLAQGEG
                     LHIVPCGSVHMFFMLFALDIIYLDREFRVVKTVSNLRPWRLSAARGAKTTLELPVGTI
                     AHTETVAGDQIVLQEL"
     gene            3928..4404
                     /gene="greA"
                     /locus_tag="VIM45_03195"
     CDS             3928..4404
                     /gene="greA"
                     /locus_tag="VIM45_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012995973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEY8820326.1"
                     /translation="MAEERVALTREGLEKLRDELQYLENVKRHEVAEALEQERESSLS
                     TEAEPGYEYAKEEQSIVEGRIAQLEHMIGNAEVIDESAAHRSGRVQLGSVVVVKPDDG
                     PEQSFQIVGSLEVDVRDGKISDESPVGSALMGHAVGDVVEVAVPHGKRRLKITSLA"
     gene            4548..>5626
                     /gene="purF"
                     /locus_tag="VIM45_03200"
     CDS             4548..>5626
                     /gene="purF"
                     /locus_tag="VIM45_03200"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008476307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEY8820327.1"
                     /translation="MMPNLDHAVTELPILSTPRELPGYLVTTVPAVETNGSVLGAAAA
                     GDDAPKEACGVFGVYAPGEDVARLTFYGLYALQHRGQESAGIATSDGNNLHLRTGMGL
                     VGHVFEEEDLAHLPGSIAIGHTRYSTTGSSRIGNAQPIHIIGPRGEIALGHNGNLVNA
                     EFLRRRLEEQGHALTSSTDSELVARMISTAPGNTWTERIQAVMRRLEGAYCLIILTPD
                     SMIAVRDPMGNRPLCVGRLGDGWVIASETCALDHLGAVYEREVEPGEVIVVDASGLHS
                     TQAVERKRDAFCIFEYIYFARPDSIIRDSRVYPLRMKMGAELAREYPVDADIVIGVPD
                     SATAAAVGYARESGIPFAEGLMKNR"
BASE COUNT         1085 a         1792 c         1755 g          994 t
ORIGIN      
        1 aaacatgatg ccgaggataa tcgccatcca ggtaagcgtc gagcgtgcgt tgtccgactc
       61 tggcttttga aacgccggca cgccgttgga gatcgcctcg acaccggtca ttgccgtaca
      121 accggaggca aaggcgcgca gaaccaagaa gatgccgatg cttgcgcccg tgctctggag
      181 cgcatgctgt tcctgcggaa tgccgccgcc gtggccaagc aggatcttga cgaaggccac
      241 gacgatcatc agcagaatgc tgaagataaa cacgtaagtt ggagccgcaa agatgctgcc
      301 cgattccttc acgccccgta ggttgatgac gaccatgaac gccagcgcgg cgagagcgat
      361 ttcaaccgta aaggggtgaa gaccctctac cgcggaggtg accgcttgga cgccggacga
      421 gatgctgacg gccacggtca ggatgtagtc gaccatcagc gagccggcgg cgatcagacc
      481 cggccaaacg ccaagctctg tgtgcgcgac gatgtacgac ccgccgccgt ttgggtatgc
      541 gtggatcgtc tgccggtagg agattgcaac aagcgccagc aacacggcga tgccgacgct
      601 gatcggaacg acgttcccga gggcgcccgt cccggcgacg acaaggacga ccacgatctg
      661 ttccgtcgca tacgcaactg aggagagcgc gtcagacgag aacacggcca gcgccttgac
      721 cttcgtcagg cgctcgtgcg cgagctgtga agtggccagc ggcgagccga tgaagcgacg
      781 ccgagtcgcc gccgtgaacc gtccgaaagc cccgcgcggg gcccccgcct cgtcagtcgc
      841 ctgcagcacg ccgggcccaa gcttacggaa actgcgtgat tgggtcgtca tggtacgcac
      901 gtatctggaa cccggacgtg agccggatcg cacttcgcgc agctcgaggt ccggacttag
      961 cttccgatac gtgccgcccg tgcgtgggcg gtcgtcgtcc gcggccgggc gagggcgccg
     1021 ttgctcgacg gcctcaactc gcggttcgcg gccccgcagt tccggtggac ggcgcgccgc
     1081 ccgggcaccg ggctgggaga tgacgcgcgg tctctgcgcg ccggcgccgt ctgccggtgg
     1141 ctgccgatcc ggattctctg gtcgctggtc ttcgggcatc cactgtccct acgctgtccg
     1201 gccgtgcgcc gtcagacgcg gtcttcatcg gccagcgtat cgcggaatat ccacacctgg
     1261 cattgcgcct gctgcaatag atggtcgatc ttggcgccca cggttggctc ggcgatgcgc
     1321 tctttcaact caaggccaag aaagatcgca tccacaccca ggttgctcgc ctcgtccacg
     1381 atgctgctcc ccgcgtccct ggcctgcaat aattcggtct cgacgccctg gccaatcgcc
     1441 gcgccgatcc gctcggcccg cgtcaggata tcgttgccgc ggcgcgtttc tgccaccatt
     1501 tcggcgtcaa gcggcagcga acgccgcaca acaatcacat ggaccgcgta cacgcggccg
     1561 gcattacggt gcgcgacgtc gcacgccaga gcaagggccg aaaaacagat gtcagatccg
     1621 tcgcacggga cgaggatatt gcgtgtgttc agtaaggcgc tggcttctgg gggcacccgt
     1681 tcggccatca gccacctccg cgctaacggg atagtatagg cgcatccaaa gcccacgtaa
     1741 caggagaccc tgaatcaagt ggcagaaatc ggcatcatcg gcggcggtgc gctcggttta
     1801 ggcgccggtc tccggctggc acaggccggg cgccgcgtcg tgatcatcga acgcgagccc
     1861 gaactcggcg ggctcgccgc gggcttcagg cccggtgaag actccaaagc ctcgctcgag
     1921 aagttctacc accacatctt caagactgac cgcaccatca tccgcaccat tgcggagctt
     1981 gggctcgaag aaaagctcgt ctggggtcaa ccagtcagtt cgctcttgcg cggcggccgc
     2041 atctatccga tgtcggtgag cggcgtgctc cggtttggcg ccattccctt cgtcgaccgc
     2101 ttgcgcttcg gcgctacggt ggcggggctc aagctcgtac caaatgaacg acgtttcgca
     2161 ggccaaacgg caacggagtg gacgcgccgc tgggccggac gtcgggcgta cgatgggttt
     2221 attgagccgc agctcgcggg caagttcggg aatcgagccc gcgacatcag catgggctgg
     2281 ctgtggagtc gcttccacga gcgtacgatc cggttgggct acctgcgtgg cggtttccag
     2341 cagctctacg acgcgattgg cgagcgaatc cgttcccttg gcggtgagat cctcctcggc
     2401 gcaagcgcct ccgccattcg cagtcgggac ggcggcgtgc aggtcgatac cccgcacgga
     2461 acgcactcgt tcgagcgact gctggtcacg gcgccgcaac gtgtatttga acggttggcc
     2521 gaaggtctac cacctgacta cgctacgagc catccaggac ccgagttcta cagcgcccag
     2581 gtgctgatcc tggcgctcga ccgcccgctg accgacaagt actggatctc cgtcggcgac
     2641 ccaggctacc cgttcctggt gcttgtcgag cacaccaact ttatgccggc ttccgactac
     2701 ggcggccgcc atctcgtgta cctgggcaac tacctgccgg acgactctcc tttgcttcgc
     2761 aagtcagaag ctgaggtgct ggccgagttt ctgccggcgc tcaagcgttt caaccccgac
     2821 ttcgaagctt cctggctgcg cgaccattgg ctgtttcgtg ccccatatgc gcaacccatc
     2881 gtcaccacca actacgtctc gtcgctgccg ccgatcgaaa caccgctccc ggccgtcctg
     2941 ctggcgacca tggcgcatgt gtacccgcag gaccgcggcc agaattacag tttgcggctt
     3001 ggcgagcaga tggccgggct gctgcttgcc ggataaatga acattaaatt acgtaatgcg
     3061 tacgggctat ataagtactg accaagggcg ccgaatcggc gataatgaac aaagggagaa
     3121 ggcaatgccc ttgaacaaat tctttgttga atacgacgaa cccgcgggcg ccgtcggatt
     3181 gatcgatgag caggtaggtc cgcttggtta ctcgtacgaa tatgccgccg cctatcggcg
     3241 ggccaagcaa ctagccaaga acggtgcgac acgggttcgg gtggtgcgcc gcaatagagg
     3301 cttgcccgaa gaggtcatct ctgaggtcgt cgcggggtag ttgtcccgac attcggccgc
     3361 gttgcacgcc ggccgcaatg gatgctgcac agagcccggc tgcgccgggc tctgtcgttg
     3421 cccgatagta gcgtagtgcc gcgtcagagg gggcaatcat ggcagcgatc cagcgcatcg
     3481 cgaatgagac ccgcgccacc gagctcggcg gccgaataca cgttgcggat aacccgtgga
     3541 cgcggtttgt agggctaatc gggagacgcg gactcgccca aggcgagggg ctgcacatcg
     3601 tcccctgcgg ctccgtgcac atgtttttca tgctctttgc gctggacatc atttacctcg
     3661 atcgcgagtt tcgcgttgtc aagaccgtct ccaatctgcg accgtggcgt ctctccgccg
     3721 cccgtggcgc gaaaaccact ctcgagctgc cggtcggaac gattgcccac acggagaccg
     3781 tggcagggga ccagatcgtg ttacaggagc tgtaactctc gcggtatcac acagcgcaga
     3841 cggtgggcta cactgtgtgc tgccgtgttg tactctacgt ggaccaatta caacaaaagc
     3901 agcaatgaac tgctgaggac aacaacagtg gccgaagaac gggttgcctt gacccgggag
     3961 ggtctggaaa agctccgaga cgaactgcag taccttgaga acgtaaagcg ccacgaagtc
     4021 gccgaggcgc tcgagcagga gcgcgaaagc agcttgagca ccgaggccga gcccggctac
     4081 gaatatgcaa aagaagaaca gtcaatcgtc gagggccgca ttgcacagct cgaacacatg
     4141 atcggcaacg ccgaagtgat cgacgaatcg gccgctcacc gatctggacg cgtccagctt
     4201 ggcagcgtcg tcgtcgtcaa gccggacgat ggccccgaac agagctttca gatcgtcggc
     4261 tcgctggagg tggacgtcag agacggcaag atcagcgacg agtcgccggt tggcagcgcc
     4321 ctgatgggac acgccgtcgg cgacgtcgtt gaggtagccg ttccacacgg caaacggcgc
     4381 ctaaaaatca cgtcactggc ctgattcgtt gttctcctgt cctgctgctt cattcagaat
     4441 ggaacggcga gagatgtagg ccgtcctgcc tccttcgtac gaagccctcg tttgacccgc
     4501 caccggcccg ctgatatcat ggtaacgcac acccagagag caggctgatg atgccgaatc
     4561 tcgaccatgc cgtgaccgaa ttaccgatcc tttccacgcc gcgagaacta cccggctacc
     4621 tggtgacgac ggtccccgcc gtggaaacaa acggctcggt actcggcgcc gcagcagccg
     4681 gcgacgacgc accgaaagaa gcttgcggcg tgttcggcgt ttacgcaccg ggggaagatg
     4741 tcgcccgact gacgttttat ggcctctacg cgctccagca ccgcggtcag gagagcgcgg
     4801 ggatcgccac ctcagacggc aacaacctgc acctgcgcac cggcatgggg ctcgtgggcc
     4861 acgtgtttga ggaagaggac ctggcgcacc tgcccggcag catcgcgatc ggacatacgc
     4921 gctactcgac cacgggctcc agccgcatcg gcaacgccca gccgatccac atcatcggcc
     4981 cgcggggcga gatagcgctc ggccacaacg gcaacctcgt caacgccgaa tttctccgac
     5041 ggcgactgga agagcaggga catgcgctca cttcctccac cgattcggag ctggtcgccc
     5101 gcatgatcag caccgccccg ggtaacacct ggacggaacg gatccaggca gtgatgcggc
     5161 gcctcgaagg cgcgtactgt ttgataattc tcacccccga ctcgatgatc gccgtgcgcg
     5221 acccgatggg taatcggccg ctctgcgtcg gccgtctggg cgacggctgg gtgatcgcgt
     5281 ccgagacgtg tgcgctggac catctcggcg cggtctacga gcgcgaggtc gagccgggcg
     5341 aagtgatcgt cgtcgatgca agcggccttc acagcacgca ggcggtggag cggaaacgcg
     5401 acgcgttctg catcttcgaa tacatctact tcgctcgtcc ggatagcatc atccgcgata
     5461 gccgcgtgta tccactgcgg atgaaaatgg gcgccgagct cgcgcgcgag tatccggtcg
     5521 acgcggacat cgttatcggc gtgcccgact ctgccactgc cgccgccgtc ggctatgcgc
     5581 gtgaatcggg catcccgttc gccgaaggcc tgatgaagaa ccgcta
//
