LOCUS       DATCQR010000001         1922 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_185855, whole genome shotgun sequence.
ACCESSION   DATCQR010000001 DATCQR010000000
VERSION     DATCQR010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1922
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185855"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..836)
                     /locus_tag="VIN32_00005"
     CDS             complement(<1..836)
                     /locus_tag="VIN32_00005"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY8920993.1"
                     /translation="MTGDQPLRIAIFGETYLPYLSGVTVSTEALARGLGAAGHEVLLV
                     VPRPAVGSEPWTAGASGPDPRIAWLPSFQAPGSAPRGYRVPKPIPSAALEAAAAFAPQ
                     IVHAQSPFTSGLMARRLARRRHAPLVFTHHTRFGDYRHYLGALAVPGSALMDAYLRRF
                     WRSCAAIVAPGNELAGEIADRVGQRPLPLVRTIPTGIDVAAMAALPAVDPRAVAGWPV
                     DSVILATLGRLAPEKSVDLVLEAFADVAATDQAVRLLMIGGGPSESSLRRRAAEPDLL
                     GRV"
     gene            complement(833..>1922)
                     /locus_tag="VIN32_00010"
     CDS             complement(833..>1922)
                     /locus_tag="VIN32_00010"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8920994.1"
                     /translation="PVVLFVLLGGVFSDRYDRRWLMIGADCVRAAAIGVLGVLSIGGV
                     IEIWHIVALIAFVGLGDAFFNPASTAIVPDLLRDDQLPRANALQALVRPMMIRLVGPA
                     VGGLVVAGVGPGTAFLVDGSSFVLSAIAIAFIGARPSRQVVSHGLRQAIAEVGEGLAF
                     VRRNPWCWATLLSAMFSLLVFIGPMQVLLPFLVKNQLGLGPDALGAIFAVGGVGSIAA
                     ALAVGHWGLPRRRITIMYAAWAGGIALFAGYGVMNSVWQALVIGFVTAALFELGQIIW
                     ITLLQTLVPRELLGRVSSLDWLVSTGLVPLSFALTGPVAAWLGPGPTMIAAGLVGSLL
                     MGALLFVPGVRDPERREIPLAETAQDMT"
BASE COUNT          365 a          682 c          663 g          212 t
ORIGIN      
        1 accctgccta gcaggtcagg ctccgcggcc cgccgacgga gcgatgactc ggacggaccg
       61 cctccgatca tgagcaggcg aaccgcctgg tcggtagcgg cgacatcggc gaaggcttcg
      121 aggacgaggt ccacgctctt ctccggggcc agtcggccaa gggtcgcaag gatgaccgag
      181 tcgacgggcc aaccggctac cgcacgtggg tcgacggcgg gcagcgccgc cattgccgca
      241 acgtcgatgc cggtggggat ggtccgcacc agcggcagcg gccgctgacc cacgcgatcg
      301 gcgatctccc ccgccagctc gttgccgggg gccacgatcg cggcgcagct ccgccagaac
      361 cggcgcaggt acgcgtccat cagcgccgag ccgggaaccg ccagcgctcc gaggtagtgg
      421 cggtaatcgc cgaagcgggt gtgatgcgtg aagacgagcg gcgcgtggcg ccgccgcgcc
      481 aggcgtcgcg ccatgagccc cgacgtgaat ggcgactgtg cgtgcacgat ctgcggcgca
      541 aaggcggccg cagcctcgag cgccgctgag gggatcggct tcggcacccg atagccgcgg
      601 ggggccgaac ccggcgcctg gaacgacggc agccatgcga tgcgcgggtc ggggcccgag
      661 gcgccggcgg tccacggctc gctccccacg gccggccgcg ggacgaccag cagcacctcg
      721 tgccccgcgg cacccaggcc gcgggcgagt gcctcggtcg atacggtcac gcccgacagg
      781 tagggcaggt aggtctcgcc gaagatcgcg atgcgcagtg gctggtcgcc ggtcacgtca
      841 tgtcctgggc tgtctcagcc agcggaatct cgcgacgctc cggatcacga acgcccggaa
      901 cgaacaggag ggcccccatc agcaatgagc cgaccagccc ggccgcaatc atggttggcc
      961 ccggtcccag ccaagccgcc acgggcccgg tcagcgcgaa tgaaagcggg acaaggccgg
     1021 tggagaccag ccagtcaagg cttgacacgc gcccgagcag ctctcgcggc acgagcgtct
     1081 ggagcagcgt gatccagatg atctgaccca gctcgaagag cgccgcggtg acgaagccga
     1141 tgacgagggc ctgccacacc gagttcatga caccgtatcc ggcgaaaagg gcgatgccac
     1201 ccgcccaagc cgcgtacatg atcgtgatgc gtcgccgtgg aagaccccag tgacccaccg
     1261 ccagggctgc cgcgatcgag cccacgcccc ccaccgcgaa gatcgcaccg agcgcgtccg
     1321 gacccaggcc aagctggttc ttcaccagga acgggagcag gacctgcatc gggccgatga
     1381 agacgagcag gctgaacatg gccgagagga gggtcgccca gcaccatgga ttgcgccgca
     1441 cgaaggcgag gccctccccc acctcggcga tcgcctggcg cagcccatgc gagaccacct
     1501 gtcggctggg acgggcgccg atgaacgcga tcgcgatcgc cgacagcacg aagctggacc
     1561 catcgaccag gaaggccgtt cccggtccga cgccggcgac caccaacccc ccaaccgcag
     1621 gtcccaccag ccggatcatc atcgggcgca ccagcgcctg gagggcgttg gcacgcggca
     1681 gctggtcgtc gcggaggaga tcagggacga tcgccgtcga agccgggttg aagaacgcgt
     1741 cgcccaggcc gacaaaggcg atcaacgcga cgatgtgcca gatctcgatc acgccgccga
     1801 tcgacaggac acccagcacg ccgatcgcag cggcccgtac gcaatcggcg ccgatcatca
     1861 accagcgtcg gtcgtaccga tccgagaaga cgccgccgag caggacgaag agaaccaccg
     1921 gc
//
LOCUS       DATCQR010000002         5541 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_139404, whole genome shotgun sequence.
ACCESSION   DATCQR010000002 DATCQR010000000
VERSION     DATCQR010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5541
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139404"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..451)
                     /locus_tag="VIN32_00015"
     CDS             complement(<1..451)
                     /locus_tag="VIN32_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8920995.1"
                     /translation="MNRVTQVLVVIGAFGVVGIVGLGALSRERLEQARAPEPTPVPLV
                     TFGAAPEGWAVGADDAVLTLARGPVAGRATVARITVCRNAYAVAADGALAHGLDTDAT
                     AFAFGLARRDDLRKVLEPHAVTLAGLDGFYLDITIAKPSQFAGETDTW"
     gene            547..1143
                     /locus_tag="VIN32_00020"
     CDS             547..1143
                     /locus_tag="VIN32_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011196152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEY8920996.1"
                     /translation="MPQRDASESWPLTLGEEPAFCPACGKALVERVLEEDHRPRLVCP
                     DGHVTWRNPRLVVGTLPVRDGRVHLARRSIEPGAGRWTYPGGFLELGEAAQEGARRET
                     EEETQLRVEVGRLLGAYSRPYAGVVTLIYEAMVVGGEVLPGVETSEVRAFGPDEIPWD
                     ELAFSTVESALRDWVASLPGRGPRFEPELYVTGDPTEG"
     gene            complement(1140..2399)
                     /gene="fabF"
                     /locus_tag="VIN32_00025"
     CDS             complement(1140..2399)
                     /gene="fabF"
                     /locus_tag="VIN32_00025"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007505035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEY8920997.1"
                     /translation="MSRDPARRAVVTGMGAITPIGNDPDTFWANLLAGVSGVAAISAY
                     DASAEEVRIAAEVKGFDPATWIDFKQARRMSRFSQFAVAAAAQAIEDGGLEVTDANRN
                     DIGVIVNTGGGGIGDVALGERVFLEQGGKRVSPFMVPMLSPSMAACQISIQNGLHGPV
                     IASVAACASGVQALIDAQRLIEHGDVEVVLAGGTESAILPVAFAALANMGALSKRNDD
                     PAGASRPFDADRDGFVFGEGAAVLMVESAEHAERRGARIIAEVAGGAMTGDAYHISAP
                     DPSGDGATRAMVKALRDAGVEPEEVDYVAAHGTSTPLNDATETKAIHSAFGEHAGHLA
                     VSSNKSMVGHTLGAAGAISAVVAVCAIRDGMIPPTINYSTPDPDCDLDYVPNTARKAP
                     VGTAIINGFGFGGQNAVAVFRRYESPG"
     gene            complement(2410..3693)
                     /gene="fabF"
                     /locus_tag="VIN32_00030"
     CDS             complement(2410..3693)
                     /gene="fabF"
                     /locus_tag="VIN32_00030"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEY8920998.1"
                     /translation="MTARRVVVTGIGVVSPVGIGAGVSWDNLVAGRSGIADITLFDTT
                     DYEIHVAGEVKGFDATQYMDGKDARRHDRNTHFAVAASGEALKDAGLLNADGMLDTAQ
                     ADADRFGMIVGTAIGGIGMIADGLATLNERGPGRVSPFLLPHMIPDAASGMVAIQYGI
                     RGPNLAVVSACATGGHAIGEAMETIVRGQADLMLGAGTEAVIVPIAFAGFGQMRALGS
                     PIDPDTGAYDPTIASRPFDLTRNGFVVSEGASVLVLEELEHARARGVTPIAEVIGYGS
                     SSDAFHMAAPAERGEGSQRSMKAALERAGIGPEAVDYVNAHGTSTPLNDRYESAAISA
                     VFGEHASSLAVSSTKSMTGHMMGAAGAFEAFVCARVVQTGCIPPTINLRHPDPELTLD
                     YVPNTARQTESRVALSNSMGLGGHNSTLILARVEA"
     gene            complement(3690..4694)
                     /gene="fabD"
                     /locus_tag="VIN32_00035"
     CDS             complement(3690..4694)
                     /gene="fabD"
                     /locus_tag="VIN32_00035"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEY8920999.1"
                     /translation="MTDQTDSSDPHDPAPQPRAHAFVFPGQGSQYVGMGAALIERSPE
                     AAAVMERADAALGFPLSRLISDGPAEELDLTVNAQPAILATSVAYLEAMRAEAATAGV
                     ELAPLRLAGHSAGQYAAAVAADSIDFETAIGLVRERGRIMQERGIEGGMGAVIGLTDE
                     QVHEVVDAAREHGEIGVANANAPGQIVLSGVIPALAFALEMSRTIGARKAVRLTVSVA
                     SHSPLMRRARDEFSQILAKVPFREPRVPMLGNVHATLIRTADGLRRELSEHLVHGVQW
                     TDSVRHMAADGVTDFVEIGPGRVLTGLIKRISPETETHALDAPGNGALWLPGSLEATA
                     "
     gene            complement(4755..>5541)
                     /locus_tag="VIN32_00040"
     CDS             complement(4755..>5541)
                     /locus_tag="VIN32_00040"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEY8921000.1"
                     /translation="VYGIQVAEKGIAWTRLHATGTTGHGSMPNPDNAAIKLAEAVTRL
                     AAAPQPARLTPVVEGFLGALGLAAVADGIRSGDEASAFAALEAGVDDPVLRRSIAAML
                     RDTVTPTMIQAGKKMNVIPGSGEAQIDVRTLPGTDQEALLAKMQATVGTLARVEPVIT
                     LPAIEAAGDAPIVELMRSALLAADHEATAAPMMITPGTDAKALATLGIPTYGFAPLQL
                     AADMPFLSLFHGHDERVPVSAIEFGLPVLHEVVSRFVTREMPG"
BASE COUNT          785 a         1928 c         1963 g          865 t
ORIGIN      
        1 gccaggtgtc ggtctcgccc gcgaactggc tgggcttggc gatggtgatg tccaggtaga
       61 agccgtccag gccagcaagc gtcacggcgt gtggctcgag caccttccga aggtcgtcgc
      121 gtctcgccag gccgaaggcg aaggcggtcg catcggtgtc caggccgtgg gccagcgcac
      181 cgtccgcggc cacggcgtac gcattgcggc agacggtgat ccgggcgacc gtggccctgc
      241 ccgctaccgg gcctcgcgcg agcgtgagca ctgcatcgtc cgcgcccacc gcccagcctt
      301 caggcgccgc gccgaaggtg accagcggga ccggagtggg ctctggcgct ctcgcctgct
      361 cgagcctctc gcgtgagagg gcgcccaaac ccacgatccc caccacccca aacgctccga
      421 tgacgaccag gacctgggtc accctgttca tcgtttgatc ctccgcactg ggcatgatgg
      481 ccgacgccgc gatgttctgt cgaccaagtc cgaccgaggt aatgcctcct gtctaccatg
      541 agcccgatgc cgcagcgcga cgccagcgaa tcctggcccc tgaccctggg cgaggagccc
      601 gccttctgcc cggcatgtgg caaggccctc gtcgagcgcg tgctggagga ggaccaccgg
      661 ccccggctcg tctgtccgga cggtcatgtc acctggcgca acccgaggct cgtcgtcgga
      721 acactgccgg tgcgcgatgg tcgcgtgcac ctcgcgcggc gctcgatcga gccgggtgcc
      781 ggacgatgga cgtaccccgg cggcttcctg gagctgggag aggctgcgca ggagggagcc
      841 cggcgcgaaa cggaggagga gacgcagctg cgggtcgagg tggggcggct gctgggcgcc
      901 tactcgcggc cgtatgccgg ggtggtgacc ctcatctacg aggcgatggt ggttggcggc
      961 gaggtgctgc cgggggtgga gacgagcgag gtccgcgcct tcgggccgga cgagatccca
     1021 tgggacgagc tcgccttctc tacggtcgag agcgcgctcc gagactgggt cgcgtcgctc
     1081 ccggggcgcg gtccccggtt cgagccggag ctgtacgtca ccggcgaccc gaccgagggc
     1141 tagccgggcg actcgtagcg ccggaagacg gccacggcgt tctgaccgcc gaagccaaag
     1201 ccgttgatga tcgcggtgcc caccggcgcc ttgcgggcgg tgtttggcac gtagtcgagg
     1261 tcgcagtcgg gatcgggcgt cgagtagttg atggtcgggg ggatcatccc gtcgcggatg
     1321 gcgcacacgg cgacaaccgc cgagatcgcc ccggccgccc cgagcgtgtg tccgaccatg
     1381 ctcttgttgc tgctcaccgc caggtgtcct gcgtgctccc cgaaggctga gtggatcgcc
     1441 ttcgtctcgg tcgcgtcatt gagtggcgtg ctcgtgccgt gggcggccac gtagtcgacc
     1501 tcctcgggct cgacgccggc atcgcgcagc gccttgacca tggcccgcgt cgcgccgtcc
     1561 ccgctcgggt caggtgcgct gatgtggtag gcgtcaccgg tcatcgcgcc ccccgcgacc
     1621 tcggcgatga tccgggcccc ccggcgctcg gcatgctcgg cggactccac catcagcacc
     1681 gccgcaccct cgccgaagac gaagccgtcg cgatcggcgt cgaaggggcg ggaggcgccg
     1741 gccggatcgt cgttgcgctt cgagagcgcg cccatgttgg ccagcgcagc aaaagcgacc
     1801 ggcaggatgg cgctctccgt cccaccggca aggaccacct cgacgtcgcc atgctcgatc
     1861 agccgctgcg cgtcgatcag tgcctggacc cccgaggcgc aggccgcgac cgacgcgatc
     1921 accggcccgt gcagcccgtt ctggatgctg atctggcagg ccgccatgga cggagagagc
     1981 atcggcacca tgaacgggct gacccgcttg ccgccctgct ccaggaagac gcgctcgcca
     2041 agcgccacgt cgccgatgcc gccgccaccg gtgttgacga tgaccccgat gtcgttgcgg
     2101 ttggcatcgg tcacctcgag cccgccgtcc tcgatcgcct gggcagctgc ggccacggcg
     2161 aactggctga agcggctcat gcgacgggcc tgcttgaagt cgatccaggt ggccgggtcg
     2221 aagcccttga cctcggcggc gatgcgcact tcctcggctg acgcgtcgta ggcgctgatc
     2281 gcggccacgc cgctcacccc cgcgagcagg ttcgcccaga aggtgtcggg gtcgttgccg
     2341 atcggtgtga tggcacccat cccggtcacg accgctcgac gggccggatc gcggctcatc
     2401 gggtggcgct catgcctcga cccgggccag gatcagcgtg ctgttgtgcc cacccagtcc
     2461 catcgagttg gagagcgcca cccgactctc ggtctggcga gccgtgttgg gcacgtagtc
     2521 gagtgtcagc tcgggatcgg ggtgccgcag gttgatggtc ggcgggatgc agccggtctg
     2581 caccacgcgg gcgcacacga aggcctcgaa ggctccggcc gcgcccatca tgtggccggt
     2641 catgctcttg gtagagctga ccgccaggct ggaggcgtgc tcgccgaaga ccgcgctgat
     2701 tgcggccgat tcgtaccggt cgttgagcgg cgtgctggtg ccgtgggcgt tgacgtagtc
     2761 gaccgcctcg ggcccgatcc cggcccgctc cagggccgcc ttcatcgagc gctggctccc
     2821 ctcgccccgc tcggcaggcg ccgccatgtg gaacgcatcc gacgaggagc cgtagccgat
     2881 cacctcggcg atcggcgtca cgccacgcgc cctggcgtgc tcgagctctt cgagtaccag
     2941 caccgatgct ccctcggaca cgacgaagcc gttgcgcgtg agatcgaagg ggcgcgacgc
     3001 gatggttggg tcgtaggcgc cggtgtccgg gtcgatcggg gagccgaggg ctcgcatctg
     3061 gccgaagccc gcgaatgcga tcggcacgat cacggcctcc gtgccggcgc ccagcatcag
     3121 gtcggcctga ccgcgcacga tcgtctccat cgcctcgccg atggcgtggc cgccggtggc
     3181 gcaggcgctg accaccgcga ggttcggccc gcgaatcccg tactggatgg cgaccatccc
     3241 cgatgcggcg tcggggatca tgtgcggcag caggaacgga gagacacggc ccggcccgcg
     3301 ctcgttcagc gtggccagtc cgtcggcgat catcccgatt ccaccgatcg cggtccccac
     3361 gatcatcccg aagcggtccg catcggcctg ggcggtgtcg agcatgccgt cggcgttcag
     3421 caggccggca tccttgagtg cctcaccgct ggcggctacc gcgaaatgcg tgttgcggtc
     3481 gtggcgccgg gcgtccttgc cgtccatgta ctgcgtggcg tcgaagccct tgacctcgcc
     3541 tgcgacgtgg atctcgtagt cggtcgtgtc gaagagggtg atgtcggcaa tgccggatcg
     3601 gcccgcgacc aggttgtccc acgacacgcc ggccccgatt ccgaccggtg agacgacccc
     3661 gatgccggtg acgaccaccc gtcgtgcggt cacgcagtcg cctcgaggga gccggggagc
     3721 cacagggcgc cgttgccggg cgcatcgagt gcgtgcgtct cggtctctgg cgagatccgc
     3781 ttgatcaggc ccgtgagcac gcgcccagga ccgatctcta cgaagtcggt caccccgtcg
     3841 gcggccatgt ggcgcacgct gtcggtccac tgcaccccgt ggacgaggtg ctcggaaagc
     3901 tcccgccgca ggccatcggc ggtgcggatg agcgtggcat gaacgttgcc cagcatcggc
     3961 acgcggggct ctcggaacgg gaccttggcc agtatctggc tgaattcgtc gcgagcgcga
     4021 cgcatcaacg gagagtggct cgccacgctg accgtcagcc gcaccgcctt gcgagcgccg
     4081 atcgtcctgc tcatctccag ggcgaaggcc agcgccggga tcaccccgga gaggacgatc
     4141 tgcccgggcg cgttggcgtt ggcgacgccg atctcgccgt gctcgcgcgc ggcgtccacc
     4201 acctcgtgga cctgttcgtc ggtgaggccg atcacagcgc ccatgccgcc ctcgatgccc
     4261 ctctcctgca tgatccgccc gcgctcgcgc acaagcccga tggccgtctc gaagtcgatc
     4321 gagtcggccg cgacggcagc ggcgtactgg ccagccgagt gcccggcgag gcggagtggt
     4381 gcgagctcga cgcccgccgt cgcagcctcg gcccgcatcg cctcgaggta ggccacgctg
     4441 gtggcgagga tcgccggctg ggcattcacc gtcaggtcaa gctcttcggc cggtccgtcc
     4501 gagatcaggc gcgacaacgg aaagcctagg gccgcgtcgg cacgctccat caccgccgct
     4561 gcctcgggcg agcgctcgat cagggcagcg cccatcccca cgtactgcga gccctggccg
     4621 gggaagacga acgcgtgggc gcgcggctgt ggagccggat cgtggggatc ggaggaatcg
     4681 gtctggtcgg tcattcgcat ctgaacgtcg gtggacttgg tcgcgagtat agccgagggg
     4741 ccctcgatcg ccgatcagcc tggcatctcg cgggtgacga atcggctcac cacctcgtgc
     4801 aggaccggca acccgaattc gatggcgctg accggcacgc gctcgtcgtg gccgtggaac
     4861 agggagagga aaggcatatc cgcggcaagc tgcagcgggg cgaacccgta ggtggggatg
     4921 cccagcgtgg ccagcgcctt ggcgtccgta cccggggtga tcatcatcgg cgcagccgtc
     4981 gcctcgtggt cggccgccag cagggcgctc cgcatcagct cgacgatggg agcgtcaccg
     5041 gcggcttcga tggccgggag cgtgatgacc ggctcgaccc gggcgagcgt gcccaccgtc
     5101 gcctgcattt tcgccaggag ggcctcctga tcggtgccgg gcagggtccg cacgtcgatc
     5161 tgcgcctcgc cgctgcccgg gatgacgttc atcttcttgc cggcctggat catggtcggg
     5221 gtgacggtgt cgcgcagcat ggctgcgatc gatcgacgca ggaccgggtc gtccacccct
     5281 gcctcgagtg cggcgaaggc agatgcctcg tcgccggaac ggatgccatc cgcgacggcg
     5341 gcaaggccca gggcgcccag gaacccctcc accaccggcg tcaggcgcgc gggctgcggg
     5401 gctgccgcca ggcgcgtcac cgcctcggcc agcttgatgg cggcgttgtc ggggttcggc
     5461 atcgagccat gcccggtcgt gccggtggca tgcagccgcg tccaggcgat ccccttctcg
     5521 gcgacctgga tgccgtacac c
//
LOCUS       DATCQR010000003         2019 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_93002, whole genome shotgun sequence.
ACCESSION   DATCQR010000003 DATCQR010000000
VERSION     DATCQR010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93002"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            288..1058
                     /locus_tag="VIN32_00045"
     CDS             288..1058
                     /locus_tag="VIN32_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015479680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY8921001.1"
                     /translation="MKGQVAVVTGAGSEAGIGFAIARRLHLAGFHVAVTSTTDRIHDR
                     ARELDLNGKTAFGFVADLTDEDAVHKLVESVLRKSRRIDVLVNNAGMAQTGRPVEEAT
                     VQQMDYADWQRQIEITLNTAFLMTRAVLPGMVAKKYGRIVNVSSVTGPLVSHPGWAAY
                     SAAKAGMDGMMRAVALETAADGVTINGVAPGWIATASSSDMEHAASLNTPIGRAGRPD
                     ELAAAVAFLASPDASYITGQSLVVDGGNILQEDKSRSR"
     gene            1115..>2019
                     /gene="speB"
                     /locus_tag="VIN32_00050"
     CDS             1115..>2019
                     /gene="speB"
                     /locus_tag="VIN32_00050"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013003400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HEY8921002.1"
                     /translation="MGDDGDKVIGPVDPMQVPRFAGPATYARLPALDAVGKAEVAILG
                     VPFDSGVVYRPGARFGPAAIRSGSRMLRPFNAWTEAEPFAQQVADAGDVACTPFGIEQ
                     AIGEIEAAAKATYERASHVMAIGGDHTIALPMLRAASKRFGPLALVHFDAHLDTWNTY
                     FGGARYTHGTPFRRAAEEGLLELRRSLHVGTRGPQFSAQDLPEDAALGFRLIDGRAID
                     DRGAASIASEIREVVGDAQVYLSLDIDVLDPAFAPGTGTPEAGGWSSREAIAIIRGLG
                     GIRLAGADVVEVAPAYDHAEVTAIAA"
BASE COUNT          309 a          629 c          744 g          337 t
ORIGIN      
        1 tggcgcgcgt gctcagcgag cgcggaatgg cggcgccgcg cgatgccaag ggcagagagc
       61 gtttctggac cagcggcgac cccgccgagt cggcgcgtgt gatgtcagcg ctcctggagc
      121 ggaaggtcag cgtcggcaag ctgcccgtct agttacgcgg ctgcgccgcg gggcgttacg
      181 cggctgcgct gcggggcgtt acgcggctgc gctgcgggcg ttacgcggct gcgctgcggg
      241 gcgttacgcg gctgcgtcgc gcccgcgcgc cgataatacc gacgcaagtg aaaggtcagg
      301 tcgcggtggt gacgggagcc ggcagcgagg ccgggatcgg atttgccatc gcgcgccggc
      361 tccacctggc gggattccac gtcgccgtca catcgacgac cgatcgcatt cacgatcgcg
      421 ctcgcgagct cgacctcaac gggaagaccg cattcgggtt tgtcgcagac ctcaccgacg
      481 aggatgcggt gcacaagctt gtcgagtcgg tgctgcggaa gtcgcgccgc atcgatgtgc
      541 tggtcaacaa cgccgggatg gctcagaccg gccggccggt ggaggaagcg accgtgcaac
      601 agatggatta cgccgattgg cagcggcaga tcgagatcac gctgaacacc gccttcctca
      661 tgacgcgcgc ggtgctaccc gggatggtgg cgaagaagta cggccggatc gtgaacgtgt
      721 cctcggtgac cgggccgctg gtcagccacc ctggctgggc cgcctacagc gcggcgaagg
      781 ccggcatgga cgggatgatg cgggcagtgg cgctggaaac cgcggcggac ggtgtcacca
      841 tcaacggggt cgcgcccgga tggatcgcga ccgcgtcctc atcggacatg gagcacgccg
      901 cctctctcaa cacgccgatc ggtcgcgcgg gcaggcccga cgagctggcg gcggcggtgg
      961 cttttctcgc ttcgcctgat gcctcttaca tcacggggca gtcgctcgtc gtcgatggcg
     1021 gcaacatcct tcaggaggac aagagccgct cccgatagtc ggtaggtagt tctagtgcag
     1081 taaggcctcg gccgtactga gtagactccc ggcgatgggt gatgacggcg acaaggtgat
     1141 cgggcctgtc gacccgatgc aggtgccccg gttcgctggt cctgccacgt acgcccgcct
     1201 gccggcgctg gatgcggtgg gcaaggccga ggtggcgatc ctcggcgttc ccttcgacag
     1261 cggtgtcgtg tacaggcccg gcgcgcgctt cgggccggct gcgatccggt ccggatccag
     1321 gatgctccgt ccgttcaatg catggaccga ggcggagccg ttcgcgcagc aggtggccga
     1381 cgcgggcgac gtcgcgtgca ctccattcgg gatcgagcaa gctatcggtg agatcgaggc
     1441 ggcggcgaaa gcgacgtatg agcgggcgtc ccacgtcatg gcgatcggcg gcgaccacac
     1501 gattgcgttg ccgatgttgc gcgcggcgtc taagcgtttc gggccgctgg ccctggtcca
     1561 tttcgacgct cacctggata cgtggaacac gtacttcggg ggcgcgcgct acacgcacgg
     1621 aactcctttt cggcgcgcag ctgaagaagg cctgctggag ctgcggcggt cgctgcatgt
     1681 agggacgcgc ggtccgcagt tctctgccca ggacctgccg gaggatgcag cgcttggctt
     1741 tcgtttgatc gatgggcggg cgatcgacga tcgcggcgca gcctcgatcg cgtccgagat
     1801 tcgggaggtc gttggcgacg cgcaggtcta cctgtcactc gacatcgacg tcctcgaccc
     1861 tgctttcgct cccgggaccg gcacgcctga ggccggaggc tggagcagcc gagaggcgat
     1921 tgcgatcatt cggggcctgg gcggaatccg gctggcgggg gctgatgtcg tcgaggtggc
     1981 gcccgcttac gaccacgcgg aggtcacggc gatcgcggc
//
LOCUS       DATCQR010000004        17357 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_217, whole genome shotgun sequence.
ACCESSION   DATCQR010000004 DATCQR010000000
VERSION     DATCQR010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 17357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17357
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1534)
                     /locus_tag="VIN32_00055"
     CDS             complement(<1..1534)
                     /locus_tag="VIN32_00055"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY8921003.1"
                     /translation="MLRLIRALGFMAPVPVVLLAMVGLSVTVVADPHLQFTFSALTIL
                     ASAAGATVVALGCWTYFGLRLSHVAAALEATLTSDHPVQLRVTGTPAERRLARAFNAA
                     SGAFVQVEARAAHDRLTGVVNRETLLTTLVSEIERAARHHKWLSVAFIDIDRFKPIND
                     TYGHHTGDAVLREIATLIRDNIRGSDLFGRYGGEEFMLILPETTPEEATVLAEHLRQL
                     VMDHPLTVAPRQGIQVTISIGVAGDVGSQLQADRLVDQADAAMYAAKSLGRNRTYRYR
                     AVDDNAPVRRAPISAERRAAATAIGQWASDTAAEALASVLAPQPHHGGQPSDLIATLA
                     TGMALHMGLPQDEIERIRIASLLHDLGKLAVPLEILDKRSALSDHEWQAIGEHPRIGQ
                     VILEQASSLREAVPTVLHHHEHFNGSGYPHGLRGKEIPLGARIVAVADAYHAMVNDRP
                     YRNARDHAHALAELRANAGTQFDPEVVDVFCEVFAEGVPPDGLQEVYNLHERARGGLR
                     RLE"
     gene            1833..3323
                     /gene="purF"
                     /locus_tag="VIN32_00060"
     CDS             1833..3323
                     /gene="purF"
                     /locus_tag="VIN32_00060"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEY8921004.1"
                     /translation="MPRYEPARYESHGPREECGVVGVYAPGMPVAHLVALGLHALQHR
                     GQESAGIAVSDGSQLTLHKRLGLVGQVFDEETLARLEADQDGAPAGHAARLAIGHTRY
                     STTGSNSLPNVQPVYASSELGELMLGHNGNLTNATWLRDELTEQGIEFVTASDTEVLA
                     KLIAHAPGRSWAQRVEQAFHRASGAYTFTMLTASALVAARDPVGFRPLALGRLHDAAG
                     RPSGWAIASESVGLDAMGATFVRDVEAGEILTIDDEGVHASRPGVSAEASDARERLCV
                     FEFVYLARPDSVIGGKLLYEARLRMGRRLAIEQPADADLVIPVPDSAVPAAIGYAEQS
                     GLPFREGLIKNRYVGRTFIQPGQTSREASVRLKFNPLPEVLAGKRVVVVDDSIVRGTT
                     TGPIVAMLRRAGAREVHVRIHSPQMRYPCYMGVDTGRRAELIAANLGLDGIRRQIGAD
                     TLGYLSQEGLLAAVGRDERRHCTACFDGNYPVDVPLDVDKFALERG"
     gene            3328..4359
                     /gene="purM"
                     /locus_tag="VIN32_00065"
     CDS             3328..4359
                     /gene="purM"
                     /locus_tag="VIN32_00065"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEY8921005.1"
                     /translation="MGQSYRAAGVDIDEAQRAVDLIARAAATTATPAVIGGVGGFGSL
                     FRFDPTTHPDPVLVASTDGVGTKLKLAIAMNRYDTIGIDLVHHCINDIAVQGADPLFF
                     LDYLAVGRLRAEVAAEIVTGIANACAAAGIALVGGETAELPDLYRGDDFDLAGFIVGV
                     VSAADVIDGSQVREGDVLLGLPSNGLHTNGYSLARRVFGEEDWSRTAPGLERTIGEAL
                     LEPHRSYLDEIRILRRALRRAGGDALAFAHITGGGWVDNIPRTLPPGLGASVELGSWP
                     VPLIFSLIQERGDIADDEMVRAFNLGIGLTCVVHPQHEEVARKALPEASRVGTVVSAA
                     PGSARVTFA"
     gene            4364..4984
                     /locus_tag="VIN32_00070"
     CDS             4364..4984
                     /locus_tag="VIN32_00070"
                     /inference="COORDINATES: protein motif:HMM:NF012760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HEY8921006.1"
                     /translation="MRLGILVSGRGSNLETVLDAIASGRLPAVEAVLVIANRPGVRAL
                     EVAARRGVPWRLLARPDFPSAGARDGAVGRALDESGCDLALLAGYDEVLRPSYFAAFG
                     GRTINIHPSLLPRHGGRGMVGLAVHAAVLAANETETGVTIHDVTPVLDAGPAILQVRV
                     PVAPGESVTDLAERVLEVEHETLVAVLSQLSASMTAAPPASIRARR"
     gene            5004..5594
                     /locus_tag="VIN32_00075"
     CDS             5004..5594
                     /locus_tag="VIN32_00075"
                     /inference="COORDINATES: protein motif:HMM:NF024183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEY8921007.1"
                     /translation="MPAGLPDPGKIWNDFITNPLVQTAGTLIVVYVVLLWLGTAYWAF
                     RDMQARSENPILPYFASALIIFFTPLLFLFAVVLYLIVRPRESLAEVYERSLAEESLL
                     AEVEKNELCPVCRDRVHADWLVCPNCRTRLHRVCPSCSRLADPEWPLCAFCGHEFQRP
                     AARPVERVMERTAPVRTAERSGDLGAEPGLRPASNP"
     gene            5609..6616
                     /locus_tag="VIN32_00080"
     CDS             5609..6616
                     /locus_tag="VIN32_00080"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEY8921008.1"
                     /translation="MEEPRPEGLWNAEPPTSTDRDPLSRHRALQVISVLMIAAGILLP
                     IIALLSSPGVVSQAQTPESVQRLIGPLLLLGLGGLLLVVGLVINAVRTLIVRAALPPE
                     RYRGPAVFVLLLLAVILGTILALGAGTTALALFDGGELSVGGSLLLLTSTQMGLLAVA
                     GGLVVAPRALAGVRIVGRTGLGRSLLIGLGVAIPAWIGATLLGALAAVVLKAIGLSEQ
                     AGPLDTFLQRGDPTVILVAFLLVAPVAEEIFFRGVVYNAWERERGPWVAVIGSAALFA
                     VIHTSLFALVPIFALGAALALLYRSTRSLAATIAMHAGFNAISVAIALLERQGILSLP
                     T"
     gene            6706..7353
                     /gene="radC"
                     /locus_tag="VIN32_00085"
     CDS             6706..7353
                     /gene="radC"
                     /locus_tag="VIN32_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEY8921009.1"
                     /translation="MQPQRQAVPGAAAKLARIGVEGLGDDELLAILLGGRDAATVARS
                     VLEPGGLAGLASAGLSDLPPMASRRAACLLAAVELGRRVASAWPVDGWRIRAPADLAE
                     RLIPAMGHLEREELRSVLLNTKNTVTGMVTVYAGNLAGSSVRIGEVFREAVRRQSAAM
                     VVVHNHPSGDPSPSAEDLRITRELAEAGRLLDIELLDHLVIGHGRWVSLRALGAL"
     gene            complement(7412..8290)
                     /locus_tag="VIN32_00090"
     CDS             complement(7412..8290)
                     /locus_tag="VIN32_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050825 - ice binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ice-binding family protein"
                     /protein_id="HEY8921010.1"
                     /translation="MKARIRNPRRKLSAGIAVALATSMLAVGQIAIAAGPPPVGLGSA
                     APFAVLAGTPAITNTKPTTITGDLGISPAAAVTGFPPGTVSGTIHAADAVALQAKIDL
                     ATAYTVAAALPVTATHGTLGGLTLVGGVYNAGGVTLDLTGTLTLDGQNDPSSVWVFQA
                     TSDLITASSSTVALINGASACNVFWQVTSSATLGSGATFVGTIMALTSITMQDSVTVT
                     GRALARNGQVTLINDSINASTCASTPTPSPSLTNVAMRGSGRGNPLGTLLFALVLTAM
                     LAALAAANVRTAHRSR"
     gene            complement(8410..9669)
                     /locus_tag="VIN32_00095"
     CDS             complement(8410..9669)
                     /locus_tag="VIN32_00095"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEY8921011.1"
                     /translation="MHASDRRQNGRATAALATLVLVGCSLPTGSPEASSTPGAPRPSS
                     LAASPSPSLSPTFGPLEAISLEPVASGLTDPDGIANAGDGSDRLFVTQRGGRIRVVNP
                     DGTLSQADFVDLSDRIKAGGEQGLLGLAFHPDFAANGRLFVHYSRAVDGATVVSELTA
                     SADHSTASAVSERILFTHDQPFANHNGGEIAFGPDGYLYIGLGDGGSGGDPYGNAQNR
                     HTLLGKILRIDVDASPVPNKRYAIPETNPYAAGGIAPGAGLPEIWAFGLRNPWRFSFD
                     RGTGDLYIGDVGQNAWEEVDRQPGDSRGGENYGWDIFEATHCSSGCDSITAVAPIAEY
                     GHGEGCSVTGGYVYGGTRQPAMLGTYLFGDYCSGTIWTPPASEGLIPRPLGDTGLRIS
                     SFGEGEDGEVYLVDLNGGGLYRVLAAG"
     gene            9722..11806
                     /locus_tag="VIN32_00100"
     CDS             9722..11806
                     /locus_tag="VIN32_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012646307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-1,4-glucan--maltose-1-phosphate
                     maltosyltransferase"
                     /protein_id="HEY8921012.1"
                     /translation="MTTTRAALTRAAASAHAALEPAPDAIGTRRSIVIEAVDPEVDCG
                     EAAVKRVVGDDLRVTADIFAEGHDLLDAALLVRREDQSRWTETAMRLVSNDRWSGTAP
                     LRHNARHRYTIEAWRDAIGSWRETLTKKLDAGQPVATELAEGRLLLEAAQLRTRGADA
                     RLIGTALKRESRARTDRTRAVALADDQVVAAARRHPDRGAATRYRLELPLIVDRELAR
                     SSAWYELFPRSQGRDPAGPVATTLREAEWRLPQIAAMGFDVVYLPPIHPIGSTNRKGR
                     DNAVRARRGDPGSPWAIGRDGGGHTEVAPELGGIEALDHFRRGAEAHGLEVALDLALQ
                     ASPDHPWVKQHPEWFRQAPDGTIKYAENPPKRYEDIYPFDFATPAAADRDALWRAWLQ
                     VVMTWVERGIRIFRVDNPHTKPVAFWRWLISEVQAVDPGVIFLAEAFTTPKRMRALAK
                     AGFSQSYTYFTWRESPEQLRDYLTELTAGPMREYFRGNLWPNTPDIFPHHLDHGRPAF
                     AIRAVLAATLAPSWGIYSGFELCEQGRLEEGEEYAHSEKYEIRARDWDARGNIKALVS
                     GLNRVRREWRALRLNDNLRFEQVSGERSLFYRKAMPAGEIDPLTGLPSLWRDPVYVAV
                     SCDPGRPESATMHPDLPAIGIGWDEPYLMVDLLTGRSRRLRGAEIRVELNPARLPYRV
                     FTIRPVAGSPAR"
     gene            11803..13809
                     /gene="glgX"
                     /locus_tag="VIN32_00105"
     CDS             11803..13809
                     /gene="glgX"
                     /locus_tag="VIN32_00105"
                     /inference="COORDINATES: protein motif:HMM:TIGR02100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HEY8921013.1"
                     /translation="MTADLAAPLGAVPDKSGTAFRLWSEHAEAVELSLFDADGTERRM
                     PLARGPDRVWAAHVQGAGEGQRYGYRVHGPFAPKDGQRFNAAKLLLDPYARLVEGPIT
                     LGDEHFSYRREGDPDRAADPRDSAPFTPKSIVQRALPAIDESERPRVPMLDSVIYELH
                     VKSFTRRHPDIPEELRGTYAGLAHPAAIAHLRGLGITAVELLPVQQQLTPRFVLERGQ
                     IDYWGYNTIAFLAPDPRLAATSDPRAELRETIRTLHAAGIEVLLDVVYNHTGEGDELG
                     PTISFRGIDNAAYYRLDPRDRSKYRNDSGTGNTLDATHPAVVRLVLDSLHTFAAEYGA
                     DGFRFDLATVLGRTRGGFSAEAPLFAAIQADPRLAGLKLIAEPWDLGVLGYRLGEFPA
                     GWSEWNGQYRDAIRRLWAGTEHLPGDVGQRIGGSPDLFPIDARGPVASLNFVTSHDGF
                     TLRDLVSYAEKHNEANGEGNQDGEATNDSLNFGVEGDTEDEGILALRDRQRRALLATL
                     LCSRGPAMVLSGDELGRTQLGNNNAYSLDNEVSWLDWTPAPRDAALLAFTQSLIALRA
                     AHPLLRHGTASVVGTGPLTVLILPSDPGEGPEDALLLALNPTDAPAVVHLPADQPRRW
                     IRYLDSAGGDPPVPSGEPLPEGETSFDLRGRSVALLGLPAASVR"
     gene            complement(13806..15677)
                     /gene="glgB"
                     /locus_tag="VIN32_00110"
     CDS             complement(13806..15677)
                     /gene="glgB"
                     /locus_tag="VIN32_00110"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF003811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HEY8921014.1"
                     /translation="MADEHQAPRPWQVLGAHLRKVGRINGVAFSVWAPNARRVTVTGA
                     FSNWHDDAVPMTLNDSTGIWECFVPGVQAGERYRYVVRGADGRTRHKSDPYGQSMDAP
                     PGSATHVPAPSAHAWSDDAWLAQRRVLDLQRAPMRIYEVHLGSWRRGPHGRFLTYADL
                     GPLLAEHCTRLGFTHIELLPLAEHPFYASWGYQVTGFHAPTARYGTPDQLRAMVDLLH
                     RSGIGVLLDWVPAHFARDRHGLARFDGTHLYEDPDPQRREHPEWGTLVFNFGRPEVRN
                     FLIGNARYWLEEFHVDGLRVDAVASMLYLDYGRKRGQWTPNALGGNHNLEAIDFLHDL
                     NTRVRLGLPGVITIAEESTDFENVSRPVAEGGLGFDFKWDLGWMHDTLVYFRKRPEQR
                     WRHRREITFRGFYLDAERWILPLSHDEVVHEKHSLLGKMRGTPTQKFASLRSLLANQV
                     GQPGKKLLFMGTELAPEGEWNHDHALPWKEADGDPARATYATFLADLAAVYHSSPSLW
                     ARDPDPEGFAWIDGEGMTDPSVVAWLRRGALPDGAEELTVVVQNGASTTRRYRLGLPS
                     VGRWEEVLNTDAQRYGGTDIGNPGPIEAEARSWGGQPASALVTIPPLGILFLRPAAG"
     gene            15817..16872
                     /locus_tag="VIN32_00115"
     CDS             15817..16872
                     /locus_tag="VIN32_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEY8921015.1"
                     /translation="MGIFDGLLGLLSHDVGIDLGTANTLVTVRHRGIVISEPSVVAMD
                     TRTRRVLAIGAEAKRMVGRTPANIVAIRPLRDGVISDFDVTEQMIRYFIQKVHDRYAR
                     IPRPRVLVGIPSGVTEVEKRAVRDATLNAGARWARLIEEPMAAAIGAGLPVSEPTGSL
                     IVDIGGGTTEVAVISLGGIVVSRSIRIGGDEMDVDIVSFARREYNLLMGERTAEEIKI
                     AVGSAYPMDNPRTVTFRGRDLLTGLPRAVEVGSDQIREALEPSVAQIIDAIKETIEET
                     PPELVADIMDQGIVLAGGGALLIGLDKRVAEATQMPVHVADDPLTCVVRGTGRVLEEL
                     DTLERVMVSEQFTRAPR"
     gene            16880..>17357
                     /gene="mreC"
                     /locus_tag="VIN32_00120"
     CDS             16880..>17357
                     /gene="mreC"
                     /locus_tag="VIN32_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005618 - cell wall [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEY8921016.1"
                     /translation="MLDGSPHRRSRAGLWFAAATVISLLLLLASRTPQALTLQEVTSH
                     ALDPVRSAITGVGAGISGFFATIGEIDRLRSENDHLREELAGADARLAELREAAAENA
                     ELRGLLGLTTSLQMDLLPVRILGRDPGNFTWEVGIDAGTSDGVRIGMPVVAAADGAG"
BASE COUNT         2551 a         6038 c         6034 g         2734 t
ORIGIN      
        1 gctcgagtcg acgaagcccg ccccgagctc gctcatgcag gttgtagacc tcctgcaggc
       61 cgtcgggcgg gacgccttcc gcaaacacct cgcagaaaac gtcgacgacc tccggatcga
      121 actgggtgcc cgcattcgca cgcagctcgg caagggcatg ggcatggtca cgcgcattgc
      181 gataggggcg gtcgttgacc atggcgtggt acgcatcagc gaccgccacg atgcgcgcac
      241 cgagcgggat ctccttgccg cgcagcccgt gtgggtagcc gctcccgttg aagtgctcgt
      301 ggtgatggag gacggtcggg accgcctcgc gcagcgagga ggcctgctcc aggatcacct
      361 gcccgatgcg tgggtgctcg ccgatcgcct gccattcgtg atcggagagt gcgctccgct
      421 tgtccaggat ctcgagcggg acggccagct tgccgaggtc gtggaggagg ctggcgatgc
      481 gaatccgttc gatctcgtcc tggggcagac ccatgtggag cgccatcccg gtcgccagcg
      541 tggcgatcag gtcggacggc tgcccaccgt gatgcggctg cggcgcgagg acggacgcca
      601 gcgcctcggc tgcggtgtcg ctggcccatt ggccgatggc cgtcgcggcc gcgcgtcgct
      661 cggccgagat cggtgcgcgg cggacaggcg cgttgtcgtc aaccgcccga tagcggtagg
      721 tccggttcct gccgagcgac ttcgccgcgt acatcgcggc atcggcctgg tcgaccagcc
      781 gatcggcctg cagctgggac cccacatcgc cggcaacgcc aatgctgatg gtgacctgga
      841 tcccctggcg cggagcgacg gtgagcggat ggtccatcac cagctggcgc aggtgctccg
      901 ccagcacggt cgcctcctcg ggcgtcgtct ccggcaggat cagcatgaat tcctcgccgc
      961 cgtagcggcc gaagagatcg ctgccgcgga tgttgtcgcg gatgagggtc gcgatctcgc
     1021 gcagcaccgc gtcccccgtg tggtgaccgt aggtgtcgtt gatcggcttg aagcggtcga
     1081 tgtcgatgaa ggcgaccgac agccacttgt ggtgacgcgc ggcccgctcg atctcggaga
     1141 caagggtggt gaggagcgtc tcgcggttga caacccccgt caggcggtcg tgcgcggcac
     1201 gagcctcgac ctgcacgaat gccccgctgg cggcgttgaa cgccctggcc agccgtcgct
     1261 cggccggcgt gcctgtcacc cgcagctgga cgggatggtc gctcgtgagg gttgcctcca
     1321 gggctgcggc gacgtgactc agccggaggc cgaagtaggt ccagcagcca agcgccacga
     1381 cggtcgcgcc ggcggcgctg gccaggatgg tcaatgcgct gaaggtgaac tgcaggtgtg
     1441 gatccgcaac gaccgtgacc gagagcccaa ccatcgccaa caggacgacg ggaaccggag
     1501 ccatgaatcc gagcgcccgg atgagtcgga gcatctgccg tccattcgcg aggggttgcc
     1561 acccttggtc gtccgaggat ggccctcccc gtggtcggct gccatgacct gatcgtcacc
     1621 tctcgcgccg cgcgagcccc gtcagcgacc ggtttcgagc cgagtgcggt gcggggcagt
     1681 actgcgccgg tacctttgtt cggggcggca tcggatgccg ggcggctccg gtatcatcag
     1741 ggcgtgcctc gatcccacct cgcccgctga tcacggtgcg acggaccgcc ccgccggctc
     1801 ctgaatccga ggctggacca caccttcgcg agctgccgcg ctacgagccg gcgcgctacg
     1861 agtcgcatgg gccccgggag gagtgcggcg tcgtcggggt ctacgctccg ggaatgccgg
     1921 tcgcgcacct ggtggcgctc gggctgcacg ccctccagca tcggggacag gagtcagccg
     1981 ggatcgccgt ctcggatgga tcgcagctca ccctgcacaa gcggctcggg ctggtcggcc
     2041 aggtcttcga cgaggagacg cttgcccgcc ttgaggccga ccaggacgga gcgccggccg
     2101 ggcacgcggc acgcctggcg atcgggcaca cccgctacag cacgacggga tccaacagcc
     2161 tcccgaacgt ccagccggta tacgcatcga gcgagctggg ggagctcatg ctcgggcaca
     2221 acggcaacct gaccaacgcg acctggctgc gcgacgagct gaccgagcag gggatcgagt
     2281 tcgtgacggc cagcgacacg gaggtgctgg ccaagctgat cgcccacgcg cccggccgca
     2341 gctgggcgca gcgcgtggag caggccttcc accgcgcgtc gggcgcgtac accttcacca
     2401 tgctcaccgc cagcgcgctg gtcgccgctc gcgacccggt cggcttccgc ccgctcgcgc
     2461 tcgggcgact ccacgacgcc gccggacgcc cctcgggctg ggccatcgcg tccgagagcg
     2521 tcggcctgga cgcgatgggc gccaccttcg tgcgcgacgt cgaggctggc gagatcctca
     2581 ccatcgatga cgagggggtc catgcgtccc ggccgggcgt cagcgccgag gcgagcgacg
     2641 ctcgcgagcg actgtgcgtg ttcgagttcg tgtaccttgc ccggccggac agcgtcatcg
     2701 gcgggaagct tctgtacgag gccaggctgc gcatgggtcg gcgcctggcg atcgagcagc
     2761 cagccgatgc ggacctggtg atcccggtgc cggactccgc ggtgccggcc gccatcggct
     2821 acgccgagca gtccggcctc ccattccgcg aaggcctgat caagaaccga tacgtgggcc
     2881 ggaccttcat ccagcccggc cagacctcgc gcgaggcatc ggtgcgactg aagttcaacc
     2941 cgctgccgga ggtgctcgcg ggcaagcggg ttgtggtggt cgatgactcg atcgtgcgtg
     3001 gcacgacgac cgggccgatc gtcgcaatgc tgcggcgggc cggcgcgcgc gaggtgcacg
     3061 tccggatcca ctcgccgcag atgcgctacc cgtgctacat gggggtggac accggccgtc
     3121 gagccgagct gatcgcggcc aacctggggc tcgacgggat ccggcgacag atcggagccg
     3181 acacccttgg ctacctgtcg caggagggcc tgctggcggc ggtcggccgc gacgagcgcc
     3241 gccactgcac cgcctgcttc gatggcaatt acccggtcga cgtgccgctc gacgtcgaca
     3301 agttcgcgct ggagcgcggc tgatccggtg gggcagagtt atcgggctgc cggggtcgac
     3361 atcgacgagg cgcagcgtgc ggttgacctg atcgcgcgcg cggcggcgac aacggcgacg
     3421 cctgcggtga ttggtggcgt cgggggcttt gggagccttt tccgcttcga cccgaccacc
     3481 catcccgacc cggtgctggt ggcgtcgacg gacggggtcg gcaccaagct gaagctggca
     3541 atcgcgatga accgctacga caccatcggc atcgacctgg tccaccactg catcaacgac
     3601 atcgcggtcc agggggccga tcccctcttc ttcctcgact acctcgccgt cgggcggctg
     3661 cgggccgagg tggccgcgga gatcgtgacc ggcatcgcca atgcctgcgc ggccgccggc
     3721 atcgcgctcg tcggcgggga gacagccgag ctgccggacc tgtatcgcgg cgacgacttc
     3781 gacctcgccg gcttcatcgt cggggtggtg tccgcggccg acgtgatcga cggctcgcag
     3841 gtgcgcgagg gcgacgtcct cctgggcctg ccgtcgaacg gcctgcatac caatggctat
     3901 tcgctcgcgc gacgcgtctt cggcgaggag gactggtcgc gtacggcgcc cggactggag
     3961 cggacgatcg gcgaggcgct gctggagccc catcggtcct acctcgacga gatcaggatc
     4021 ctgcgtcgcg cactgcgtcg tgctgggggt gacgcgctcg cctttgcgca catcaccggc
     4081 ggcggctggg tcgacaacat cccgcgcacg ctgccgccag ggctgggggc ctcggtcgag
     4141 ctggggtcat ggccggtccc gctcatcttc agcctgatcc aggagcgcgg cgacatcgcg
     4201 gacgacgaga tggtgcgcgc cttcaacctg gggatcggcc tcacctgcgt cgttcacccg
     4261 cagcacgagg aggtggcgcg caaggcgctc cccgaggcca gccgggtcgg cacggtggtg
     4321 agcgccgcgc cggggtccgc gcgcgtcacc ttcgcctagg ccgatgcgcc tcggcatcct
     4381 ggtttcgggt cgcggctcga acctggagac ggtccttgat gcgattgcct ccgggcgatt
     4441 gccggcggtc gaggcggtcc tggtgatcgc caaccggccg ggagtccggg cgttggaggt
     4501 ggccgcccgc cgtggcgtgc catggcgcct tctcgcgcgg cccgacttcc cgagcgccgg
     4561 agcgcgggac ggcgcggtcg gtcgcgccct ggatgaatcg gggtgcgacc tggcgctgct
     4621 ggccggatac gacgaggtcc tgcgaccttc gtactttgcc gcgttcggcg gtcgcaccat
     4681 caacattcat ccctcgctgc tccctcgcca tggcggccgt gggatggtcg ggctcgccgt
     4741 gcacgccgcc gtgctggccg cgaacgagac ggagacgggg gtcacgatcc acgacgtgac
     4801 gccggtcctc gacgccggcc cggcgatcct gcaggtccgg gtgccggtcg cgcccggaga
     4861 gtcagtcacg gatctggccg agcgtgttct cgaggtcgag cacgagacgc tggtggccgt
     4921 gctttcgcag ctatctgcta gcatgaccgc cgcgccgcca gcttcgatcc gcgcgcggcg
     4981 ataacgcaga gaggcgcctt cgcatgccag ccggcctgcc ggatccgggg aagatctgga
     5041 acgacttcat caccaacccg ctggtgcaga ccgcgggaac gctcatcgtc gtatacgtcg
     5101 tcctgctgtg gcttgggacc gcctactggg ccttccgcga catgcaggcg cgctcggaga
     5161 acccgatcct tccctacttc gcctcggcgc tgatcatctt cttcacgccc ctgctgttcc
     5221 tgttcgccgt ggtcctgtac ctcatcgtcc ggccacgcga gtcgctggca gaggtctacg
     5281 agcgctccct cgccgaggag tcgctgctgg ccgaggtcga gaagaacgaa ctctgccccg
     5341 tctgtcgcga ccgggtccac gccgactggc tggtctgccc gaactgccga acgcggctcc
     5401 accgcgtgtg cccctcctgc agccggctcg ccgatcccga gtggccgttg tgcgccttct
     5461 gcggtcatga atttcagcgt ccggcggcgc gcccggtcga gcgtgtgatg gagcgcaccg
     5521 ccccggtgcg caccgccgag cgcagcggcg acctgggcgc cgaacccggc ctgcgccccg
     5581 cctctaaccc ctgaccggcg gcccgccggt ggaggagccc cggcctgaag ggctgtggaa
     5641 tgcggagcct ccgacctcga ccgaccgcga cccgctgagc cgccaccgcg cgctgcaggt
     5701 catctcggtg ctgatgatcg cggccggcat cctgctcccc atcattgccc tgctgagcag
     5761 cccgggcgtc gtaagccagg cccagacgcc tgagtccgtc cagcggctga tcgggccgct
     5821 cctgctgctc ggcctgggtg gcctgctgct tgtcgtcggc ctggtcatca acgcggtgag
     5881 gacgctgatc gtgcgcgcgg cccttcctcc tgagcgctat cgcgggcctg ccgtcttcgt
     5941 gctgctgctg ctggctgtca tcctcgggac gatcctggcg ctcggggctg gcaccaccgc
     6001 gctggcgctc ttcgacggtg gggagctttc ggtgggcggc tcgctgctcc tcctcacctc
     6061 gacccagatg ggcctgctgg cggtggcagg cggactggtg gtggcccccc gggccctggc
     6121 cggcgtccgg atcgtcgggc ggaccggcct gggacggagc ctgttgatcg gcctcggagt
     6181 ggcgatcccg gcgtggattg gcgcgaccct gctcggcgcg ctggccgccg tcgtgctcaa
     6241 ggcgatcggc ctctccgagc aggcgggtcc gctggatacg ttcctgcagc ggggcgaccc
     6301 gaccgtcatc ctcgtggcgt ttctgctggt cgcgccggtg gcggaggaga tcttcttccg
     6361 cggcgtggtc tacaacgcct gggagcgcga gcgcgggccg tgggtggcgg tcatcggctc
     6421 agctgccctc ttcgccgtga tccacaccag cctcttcgcc ctggtcccga tctttgcgct
     6481 gggggccgcc ctggctctcc tgtaccgctc gacccgcagc ctggccgcca ccatcgccat
     6541 gcacgcgggg ttcaacgcga tctcggtcgc catcgcattg ctcgaacgcc aggggatcct
     6601 gtcgctcccg acatgaccga taagccgctg ataatcccgc ggtgaggcgc gcgcgctagc
     6661 ctcgccacgg tgtccgcagt gcttcgaccg atcgccttcc ccaccctgca gccgcagcgg
     6721 caggctgtcc caggagccgc ggccaagctc gctcgtatcg gagtcgaggg cctcggcgac
     6781 gacgagctgc ttgcgatcct actcggcggc cgcgacgccg cgaccgtcgc ccgatcggtg
     6841 ctcgaaccgg gcggtctggc cgggctggca tccgcgggcc tgtcggacct gccgccgatg
     6901 gcgagtcggc gagcagcatg cctgctggcc gccgtggagc tcggccgacg cgtggcctcg
     6961 gcgtggccag tcgatggctg gcgcattcgc gcccctgccg acctggccga gcggctcatc
     7021 cccgccatgg gtcatctcga gcgcgaggag ctgcgcagtg tgctgctcaa caccaagaac
     7081 acggtgacgg gcatggtgac cgtctatgcg gggaaccttg caggctcgtc ggtccggatc
     7141 ggcgaggtct ttcgggaggc ggttcgtcgc cagtcggcgg cgatggtggt cgtccacaac
     7201 cacccgtcgg gtgacccgtc gccatcggcc gaggacctgc gcatcacgcg ggagctggcc
     7261 gaggcggggc ggctgctcga tatcgagctg ctcgaccacc tggtgatcgg ccatgggcgc
     7321 tgggtcagcc tgcgcgcgct cggtgcgctg tagctcccat cggtccattg ccgtgcccaa
     7381 cctgcctcgc aggttgggcg acttgtcggg cctaccggct cctgtgagcc gttcgcacgt
     7441 tcgccgcagc cagggcggcc agcatggcgg tcaggaccag cgcgaagagc aacgtaccca
     7501 gggggttgcc tcgccccgag cctcgcatcg caacgttagt cagggagggg cttggcgtcg
     7561 gcgtgctcgc gcaggtcgac gcattgatgc tgtcgttgat cagggtcacc tgcccgtttc
     7621 gggccagcgc tcgaccggtc accgtcacgc tgtcttgcat cgtgatcgat gttagggcca
     7681 tgatcgtccc cacgaaggta gcgcccgatc cgagcgtcgc cgagctggtg acctgccaga
     7741 agacgttgca cgcgctcgcg ccgttgatga gcgccaccgt actggatgac gccgtgatga
     7801 gatccgacgt tgcctggaag acccacaccg agctcgggtc gttctggccg tcaagcgtga
     7861 gggtccccgt gaggtcgagc gtgacgcccc ctgcgttata gacgcccccg acgagggtca
     7921 gaccacccag cgtcccgtgg gtagccgtca ccggcagcgc tgcggctacg gtgtacgcag
     7981 tggcaagatc gatcttggcc tgaagggcga cagcgtcggc cgcgtggatc gtcccactca
     8041 ccgtcccggg cgggaatccc gtcacggcgg ccgccgggct gatgccgagg tctcccgtaa
     8101 tagtggtcgg cttggtgttg gtgatggccg gcgtgcccgc caggaccgcg aagggcgccg
     8161 ccgatcccag gccgacaggc ggcggaccag cggcaattgc gatctgtcca acggcaagca
     8221 tgctggtcgc gagggcgact gcgatgccag ccgagagttt tcgcctggga ttgcgaattc
     8281 gtgccttcat cgtgcctacc tcgaatcagc gaactgcccc acccttcgtg gcagtagagg
     8341 cagtctactc ctggctcagc gccgtggacc gatggaccga cgtcgtcaat ggtgcggagc
     8401 gttatcccct cagccggcgg cgagcacccg gtagaggcct ccgccgttca ggtcgacgag
     8461 gtacacctcg ccatcctctc cctcgccgaa cgacgatatc cggaggccgg tgtcacccag
     8521 tggtcggggg atcaaccctt cggatgcagg cggggtccag atcgtgccgg agcagtagtc
     8581 accgaacagg tacgtgccca gcatcgccgg ctggcgcgtg ccgccgtaca cgtagccacc
     8641 cgtgaccgag cacccttcgc catgcccgta ctcggcgatc ggcgcgaccg cggtgatgct
     8701 gtcgcatccg ctcgagcagt gcgtcgcctc gaagatgtcc cacccgtagt tctcgccacc
     8761 gcgggaatcg cccggctggc ggtccacctc ctcccaagcg ttctggccca catcgccgat
     8821 gtagaggtcc cccgtcccgc gatcgaagct gaagcgccag gggttgcgga ggccgaaggc
     8881 ccagatctcg ggcagccctg cacccggggc aatcccgccc gcggcgtacg ggttcgtctc
     8941 cgggatggcg tatcgcttgt tcggtacggg cgaagcgtcg acgtcgatgc gcaggatctt
     9001 gccgagcagc gtgtggcgat tctgcgcgtt gccataggga tcgccgcctg accctccgtc
     9061 gccaaggccg atgtagaggt agccatcggg gccaaaggcg atctctccgc cgttgtggtt
     9121 tgcaaagggc tgatcgtgcg taaagaggat ccgctcgctg accgcgctgg ccgtgctgtg
     9181 atccgccgac gcggtcagct ccgagacgac cgtcgcgccg tcgacggccc ggctgtaatg
     9241 cacgaagagc cgtccgttgg cggcgaagtc gggatggaag gcaagcccga gcagcccctg
     9301 ctcgccgccg gccttgatgc ggtccgagag gtcgacgaaa tcggcctggc tcagcgttcc
     9361 gtccgggttg acgacccgga tccggcctcc acgctgggtg acgaacagcc gatcgctgcc
     9421 gtcacccgcg ttcgcgatcc cgtcggggtc cgtcagccct gacgccaccg gctccaagct
     9481 gatcgcctcc agtggcccga aggtcgggct gagcgacgga gagggggaag cggccaggga
     9541 cgacgggcgc ggcgcgccag gagtggacga cgcctcaggg ctgccggtcg gcaagctgca
     9601 gccaaccagc acgagagtcg ccagcgcggc cgtggcccgg ccgttctggc gtcggtcgct
     9661 ggcatgcatg tcgcccacag cctacccggg gttggatggg gtacggttgc gctccaagcc
     9721 gatgacgacg acccgagctg ccctcacgcg tgccgccgca tccgcccatg ccgcgctcga
     9781 gcccgcgcct gacgccatcg ggacgcgccg ctcgatcgtg atcgaggccg tcgaccccga
     9841 ggttgactgt ggcgaggccg cggtcaagcg ggtggtggga gatgacctgc gcgtcaccgc
     9901 cgacatcttc gccgagggcc acgacctgct ggacgcggcg ctcctcgtcc gccgcgagga
     9961 tcagtcgcgc tggaccgaga ccgccatgcg cctcgtctcc aatgaccgat ggagcggcac
    10021 cgctccgctc cgtcacaacg ccaggcatcg ctacacgatc gaggcctggc gagatgccat
    10081 cggctcgtgg cgcgaaacac tcaccaagaa gctcgacgcc ggccagccgg ttgcgacgga
    10141 gctggccgag ggacggctgc tcctcgaggc ggcgcagctg cgcacgcgcg gggcggacgc
    10201 acgactgatc ggcacagcct tgaagcgaga gagtcgcgcc cgcaccgatc gcactcgtgc
    10261 agtcgccctc gcggacgacc aggtggtcgc agcagcccgg cgacaccccg atcgcggcgc
    10321 ggccacgcgc taccggctgg agctgcctct catcgttgat cgcgaactcg cgcgcagctc
    10381 ggcttggtac gagctgttcc cgcgttcgca gggccgcgat cctgccgggc cggttgcgac
    10441 gaccctgcga gaggcggagt ggcgtctccc gcagatcgcc gcgatgggct tcgacgtcgt
    10501 ctacctgcca cccattcacc cgatcggcag caccaatcgc aagggacgcg acaacgcggt
    10561 gcgggcgcga cggggcgatc caggctcacc gtgggcgatc gggcgcgatg gcggcggcca
    10621 caccgaggtt gcccccgagc tcggcggcat cgaggcgctc gatcacttcc gcaggggagc
    10681 cgaggcgcat gggctcgagg tggcgctcga cctcgctctc caggcgtcgc ccgaccatcc
    10741 gtgggtcaag cagcatccgg agtggttcag gcaagcgccg gacggcacca tcaagtacgc
    10801 ggagaatccg ccgaagcgct acgaggacat ctacccgttc gacttcgcga cgcccgcggc
    10861 cgccgatcgc gacgcgctct ggcgcgcctg gctccaggtc gtcatgacct gggtcgagcg
    10921 cggcatccgc atcttccgcg tcgacaaccc acacaccaag ccggtcgcat tctggcgatg
    10981 gctgatctcc gaggtgcagg ctgtcgatcc cggcgtgatc ttcctggccg aggcgttcac
    11041 gactcccaag cggatgcgcg ccctggccaa ggctgggttc agccagagct acacctactt
    11101 cacctggcgc gagtcacccg aacagctgcg cgactacctg accgagctga cggccgggcc
    11161 gatgcgcgag tacttccgcg gcaacctgtg gccaaacacg cccgacatct tcccgcacca
    11221 cctcgatcac ggccgccccg cctttgcgat tcgggcagtc ctcgctgcga cgctcgcccc
    11281 gtcgtggggg atctactcgg gcttcgagct gtgtgagcag ggacggctcg aggaggggga
    11341 ggagtacgcg cacagcgaga agtacgagat ccgcgcgcgg gactgggacg cgcggggcaa
    11401 catcaaggcg ctggtctcgg ggctgaatcg cgtgcgtcgc gaatggcggg cattgaggct
    11461 caacgacaac ctccgcttcg agcaggtcag cggggaacgc tccctcttct atcgcaaggc
    11521 gatgcccgct ggcgagatcg atccactcac cgggctccca agcctctggc gcgacccggt
    11581 ctacgtagcg gtcagctgcg accccggccg cccggagagt gcgaccatgc atcctgatct
    11641 gcccgcgatc gggatcggct gggatgagcc gtacctgatg gtcgacctcc tgaccggacg
    11701 ttcccgacgg ctacgcggcg cggagatccg ggtcgagctc aacccggctc gcctcccgta
    11761 tcgggtcttc accattcgcc cggtcgccgg ctcaccggcc aggtgacggc cgacctcgcc
    11821 gcgcccctgg gggcggtgcc ggacaagagc ggcacggcct ttcgcctgtg gagcgagcat
    11881 gccgaggcgg tcgagctctc cctcttcgac gcggacggca cggagcgacg catgccgctg
    11941 gcccgcggcc cggaccgggt gtgggcggcg catgtccagg gcgcgggcga gggccagcgc
    12001 tatggctacc gcgtgcatgg accgttcgcg ccgaaggacg gccagcgctt caacgcagcg
    12061 aagctgctgc tggacccgta tgcgcggctg gtcgagggac cgatcacgct gggcgacgag
    12121 catttcagct atcgccggga gggtgatccc gaccgcgccg ccgaccctcg cgactccgcg
    12181 cccttcacgc cgaagagcat cgtccagcgg gcgctgccgg ccatcgacga gtcggagcga
    12241 ccgcgcgtcc cgatgctcga ctccgtcatc tacgagcttc atgtcaagag cttcacgcgc
    12301 cgtcatcccg acatccccga ggagctgcgg ggcacctatg ccggcctggc ccaccccgcc
    12361 gcgatcgcgc acctgcgcgg cctggggatc accgccgttg agctgctgcc ggtgcagcaa
    12421 cagctgacgc cccgtttcgt gctcgaacgc ggccagatcg actactgggg ctacaacacg
    12481 atcgccttcc ttgctcccga tccgcgcctc gcggccacct ccgaccctcg ggcagagctg
    12541 cgcgagacga tccggacact ccacgcggcg gggatcgagg tcctgctcga cgtcgtctat
    12601 aaccacaccg gcgagggcga cgagctgggt ccgacgatct cgttccgcgg gatcgacaac
    12661 gccgcctact accgcctcga cccgagggac cggagcaagt atcgaaacga ctcgggcacc
    12721 ggcaacacgc tggacgccac gcatcctgcg gtcgtgcgcc tggtcctcga cagccttcac
    12781 accttcgcag ccgagtacgg cgccgatggc ttccgcttcg acctggccac ggtcctgggt
    12841 cgcacccggg gaggcttctc ggccgaggca ccgctctttg cggcgatcca ggcagacccg
    12901 cggctggccg gtctgaagct gatcgctgag ccatgggacc tgggcgtgct cggctaccgg
    12961 ctgggagagt ttccggccgg ctggagcgag tggaacgggc agtaccgcga cgccatccgg
    13021 cgcctctggg ccggaaccga gcatctcccc ggcgatgtgg gccagcgtat cggcggctcg
    13081 cccgacctct ttccgatcga cgctcgcgga ccggtggcat cgttgaactt cgtgacctcg
    13141 cacgacggct tcacgctgcg cgacctggtc agctacgcgg aaaagcacaa tgaggcaaac
    13201 ggggagggca accaggacgg cgaggcgacc aacgactcgc tgaacttcgg agtggagggt
    13261 gacaccgagg acgagggaat cctggccctt cgcgatcgtc agcggcgagc gctcctcgcc
    13321 acgctcctct gctcgcgtgg cccggcaatg gtcctctccg gcgatgagct cggccggacg
    13381 cagctgggca acaacaacgc ctacagcctg gacaacgagg tcagctggct cgactggact
    13441 cccgccccgc gggatgccgc tctacttgcc ttcacgcagt cgctcatcgc cttgcgcgcc
    13501 gctcacccgc tcctgcggca cggaaccgcg tcggtcgtcg gcaccgggcc gctgaccgtg
    13561 ctgatcctcc catcggatcc cggcgagggg cccgaagacg cgctgctgct ggcactgaac
    13621 ccgaccgatg ccccggccgt cgtccacctc cccgctgacc agccccgccg gtggatccga
    13681 tacctcgact ccgcgggcgg ggatccgccc gtgccgtcag gtgagccgct cccggaggga
    13741 gagacgagct tcgatctgcg cgggcgaagc gtcgctctcc tcgggctgcc tgctgcgtcg
    13801 gtccgctagc cggcggcagg gcgcaggaag aggatcccga gcggtgggat ggtgaccagc
    13861 gccgacgcgg gctgtccgcc ccaggaccgg gcctcagcct cgatcggccc aggattcccg
    13921 atgtcggtgc cgccgtagcg ctgagcgtcg gtattgagca cctcttccca tcgacccaca
    13981 gaggggaggc caaggcggta tcggcgcgtc gtggatgcgc cgttctggac gaccactgtc
    14041 agctcctcgg ccccgtcggg cagcgcgccg cggcgcagcc aggcaaccac ggagggatcg
    14101 gtcatccctt cgccgtcgat ccaggcgaag ccctcggggt ccgggtcgcg cgcccagaga
    14161 gacggggacg agtggtagac agctgccagg tcggcgagga aggtcgcgta ggtcgcccgc
    14221 gcggggtcgc cgtcagcctc cttccagggg agggcatggt cgtgattcca ctccccctcg
    14281 ggagccagct ccgtgcccat gaagaggagc ttcttgcctg gctgaccgac ctggttggcg
    14341 agcagcgatc ggaggctggc gaacttctga gtgggcgtgc cgcgcatctt gccgaggagc
    14401 gagtgctttt cgtgcaccac ctcgtcgtgc gacagcggca ggatccagcg ctccgcgtcg
    14461 aggtagaagc cgcggaaggt gatctcgcga cggtggcgcc agcgctgctc tggccgcttg
    14521 cggaagtaga ccagtgtgtc gtgcatccag cccaggtccc acttgaagtc gaagcccaga
    14581 ccaccttcgg cgaccgggcg gctgacgttc tcgaaatcgg tcgactcctc ggcgatggtg
    14641 atcaccccgg gcagccccag gcgaacgcgc gtgttcagat cgtgcaggaa gtcgatcgcc
    14701 tcgaggttgt gattgccacc cagggcgttc ggtgtccact ggccgcgctt gcgcccgtag
    14761 tcgaggtaga gcatcgatgc gacggcgtcg acgcggagcc cgtcgacgtg gaactcctca
    14821 agccagtagc gggcgttccc gatcaggaag ttacgcacct ccgggcgccc gaagttgaat
    14881 accagggtgc cccattcagg gtgctcgcgg cgctgcgggt caggatcctc gtacaggtgc
    14941 gttccgtcga agcgcgccag cccgtggcga tccctcgcga aatgggccgg cacccaatcg
    15001 aggagcacgc caatcccgga ccgatgcagc aggtcgacca tcgcccgcag ctggtctggc
    15061 gttccgtagc gggcggtcgg agcgtggaag cccgtcacct ggtagcccca cgatgcgtag
    15121 aacgggtgct cggcgagggg aagcagctcg atatgggtga agccgagtcg ggtgcagtgc
    15181 tcggccagga gcggccccag atcggcgtat gtcaggaacc tgccgtgcgg gccgcgccgc
    15241 cacgagccca ggtggacctc gtagatccgc atcggggctc gctgcaggtc gagcactcgc
    15301 cgttgcgcca gccatgcgtc gtcggaccac gcgtgggccg atggcgccgg cacgtgggtc
    15361 gcactgccgg ggggcgcgtc catcgactgc ccgtacgggt cggacttgtg ccttgtccgt
    15421 ccgtcggcgc cacgcacgac gtagcggtat cgctcacccg cctgcacgcc gggcacgaag
    15481 cactcccaga tgcccgtgga gtcgttcaag gtcatcggga cggcgtcgtc gtgccagttg
    15541 ctgaaggcgc cggtcacggt cacccgtcgc gcattgggcg cccagaccga gaacgccacc
    15601 ccattgatcc tccccacctt gcggaggtgc gcgccgagga cctgccaggg tcgcggggcc
    15661 tgatgctcgt cagccattgc gcgtcaccat agcgaaacgg cggcggaggg cgacttcgca
    15721 ttgcccgcgg ctggccgatc cgtcacactt gacccatccc cagccgccca gctggagtcc
    15781 cctcagcacc tcatcaccgt cccagctgga ggcaccttgg gcatcttcga cggcctgctc
    15841 gggctcctct cgcacgacgt cgggatcgac ctcggcaccg ccaacacgct cgtgaccgtc
    15901 cgtcatcgcg ggatcgtgat ctccgagccg agcgtggtgg ccatggacac ccgcaccagg
    15961 cgtgtgctgg cgatcggcgc cgaggcgaag cggatggttg gccgtacacc ggccaacatc
    16021 gtcgccatcc ggccgttgcg cgacggggtc atcagcgact tcgacgtcac cgagcagatg
    16081 atccggtact tcatccagaa ggtgcacgac cgatacgccc gcatcccgcg gccacgcgtc
    16141 ctggtcggca tcccgtcagg cgtgaccgag gtcgagaagc gtgctgtgcg cgatgcgacc
    16201 ctcaacgccg gagcgcgctg ggcccgcctg atcgaggagc cgatggcggc cgcgatcggc
    16261 gccgggctac cggtcagcga gccgaccggg tcgctcatcg tggacatcgg tggtggcacc
    16321 accgaggtcg cggtcatcag cctgggtgga atcgtggtca gccgctcgat ccggatcggc
    16381 ggcgacgaga tggacgtgga tatcgtcagc ttcgcccgcc gcgagtacaa cctgctgatg
    16441 ggggagcgca ccgcggagga gatcaagatc gccgtgggat cggcctatcc gatggacaac
    16501 ccgcgcacgg tcaccttccg tgggcgcgac ctgctgacgg gcctgccgcg cgctgtcgag
    16561 gtggggagcg atcagatccg ggaggcgctc gagccgtcgg tggcccagat catcgatgcc
    16621 atcaaggaga ccatcgagga gacgccaccc gagctggtgg cggacatcat ggaccaggga
    16681 atcgtgctcg ccggcggtgg cgcgctgctc atcggcctcg acaagcgggt cgcagaggcg
    16741 acccagatgc cggtccacgt cgccgacgac ccgctcacct gcgtggtgcg tgggaccggg
    16801 cgggtcctgg aggagctcga caccctcgag cgggtgatgg tgagcgagca gttcacccgc
    16861 gccccccgct agggctggca tgctcgacgg cagtccgcac cggcgctcgc gggcagggct
    16921 ctggtttgcg gcggcaaccg tcatctcgct gctcctgctg ctcgcctccc gcaccccgca
    16981 ggcgctgacc ctgcaggagg tcacctcgca tgcgctcgac ccggtgcgca gcgcgatcac
    17041 tggcgtcggc gcggggatct ccggcttctt cgcgaccatc ggcgagatcg accggctgcg
    17101 atccgagaac gatcacctgc gcgaagagct ggcgggtgcg gacgcccgtc ttgccgagct
    17161 gcgcgaagcg gctgccgaga acgctgagct acggggcctg ctcggcctca ccacctcact
    17221 ccagatggat ctgctgccgg tccgcatcct tgggcgcgac cccggcaact tcacctggga
    17281 ggtcgggatc gacgcaggca caagcgacgg cgtccgcatc gggatgccgg tggtggccgc
    17341 cgccgacggg gcgggag
//
LOCUS       DATCQR010000005        11641 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_217035, whole genome shotgun sequence.
ACCESSION   DATCQR010000005 DATCQR010000000
VERSION     DATCQR010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 11641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11641
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217035"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..985
                     /locus_tag="VIN32_00125"
     CDS             <1..985
                     /locus_tag="VIN32_00125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921017.1"
                     /translation="WANLHAGWVLLFLFGAAVLVGEVVDRALRREPAGKPAMSWGQIR
                     DLAVALLVSLAALALNPNGVALFAYPFDTVGITALKRYVMEWFPADLGSIFGWLLLGF
                     VVVGVAPTFAFARHRLRTSDALILVGLTVMAWQAIRFLLIIGPIGGAIVAVVLSPVIA
                     QSRVGRGMSGILARLARPVGGRRGALNAGIAAILLVLGIGVALVRTMPTAQAAEIARG
                     LPVDAVAWMDANDPGTRMFNRYEWGGYIGQHRPDQLIFMDGRADLYGDELLQMYVAII
                     GLHGDPQVTFDRYEIDYAVFPPDTPLADWFDSSAAWKRVYLDQAAAIWVRR"
     gene            985..2601
                     /locus_tag="VIN32_00130"
     CDS             985..2601
                     /locus_tag="VIN32_00130"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HEY8921018.1"
                     /translation="MRRDLVILFVLALVTRLLAALLVDYPPYGDPAYYQMIAEQLAAG
                     HGFSAPVLWSFLEVGGRLPVDPMLPIPSNGHWMPLTSIVAAGSMAVFGDLLGSYRAAQ
                     LPMILLGAVLVPFTYYVAIDLWGSRFSAWIAALLVLTAGPLLVMAPLVDAFVVFGAAG
                     SAAIWCSIRAVRAGRPGPWLVAAGAFVGLATLARVDGLLLAAAPAVAWAVHPEWPPRS
                     GRLAWGFASFLAALAVLAPWFARDLSVFGSPLPSAGGHTLWITSYNQQFSIAGDPSLA
                     EYLAWGPANIIGSKLAAWGELVGRTAVLLGGIFILPFAFGLWRERHRRELQPFLAYFV
                     VMFLAMGAVFTFHAPKGAFYHSSLAWLPFGAPLAVASLAPMATAAGRAWPFLRRPATH
                     RFLAVAGLIGAIVLSLVGSAVLLAQWSDTHARLRQAAQFLADRDAQAQVVMAYDPAAL
                     HALSGNPGVAPPFDLFPVIGQVAGAYDVRWVVVTLAPGETRDPLGLWDGAAATDAAGN
                     HPDFLPAAPDFDSPGVRVFRVVDEAPPPSD"
     gene            2707..3741
                     /locus_tag="VIN32_00135"
     CDS             2707..3741
                     /locus_tag="VIN32_00135"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEY8921019.1"
                     /translation="MPRTARPRTPSNAPIPLPSSFDQLRAQASESVTRNAWRLREVAE
                     IYREQYRTRLDEWVQLRARVDHRVAAARGTGPSPSVLRADRDALGRELAQLQSGLKRI
                     DIAARQMELVLTYLSSDDERASEPTLSDADLSPAAISLKIIQAQEGERQRLAEEVHDG
                     PAQVLTNAIFQVEYLDRVIDDSPETARHELAFLRTMLREGLDEVRSFITALRPPSVDV
                     GLKQAITAAGAEFAAKHGIAVEVSVPGIDSHVPGEAKAPMLRIVQEALQNVRKHAAAT
                     SVRISLEHGHLVIVDNGRGFDVMRLASASRNFGLQFMRERAELMGSSLQIESRQGEGT
                     RILLRIPEVH"
     gene            3750..4505
                     /locus_tag="VIN32_00140"
     CDS             3750..4505
                     /locus_tag="VIN32_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY8921020.1"
                     /translation="MAAKEKKMVEQETERRKGADRRAAGAKARIVIVDGHTLFRRGVR
                     NILELEDDMEVVGEAGNGREAITTIQELTPDVVLMDLTLPTPNGIETTQRVKRELPHT
                     AIIVLAPNEDEDQLFEAIKAGAAAYVLKDIDPTDLIAIIRRVRSGEYLINDKVFSKPA
                     VASRVLKEFRELAVYGADAQPIFAPLSPREVEILDNIAQGMTNKQVAYALGISEQTVK
                     NHMSSILRKLSVNDRTQAVVYAMRQGWIKIPED"
     gene            4817..4999
                     /locus_tag="VIN32_00145"
     CDS             4817..4999
                     /locus_tag="VIN32_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HEY8921021.1"
                     /translation="MIQFISTFIARFHREDEEGQTLVEYGLLLALIAIIVIVALLFLG
                     PIVSQIFQNVGDNLNR"
     gene            5220..6095
                     /gene="cpaB"
                     /locus_tag="VIN32_00150"
     CDS             5220..6095
                     /gene="cpaB"
                     /locus_tag="VIN32_00150"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HEY8921022.1"
                     /translation="MKRSNRLVILVGVLLAVLAFVGIVILLGQPKAETPKKPTVDVLV
                     AKQDIAIGDSVTPDLVEVKPVDPAAVSGTRIADPSQLSGAPALFPIGAGSQVSQEAVG
                     LGTNTNIPCIVCQLQPGEKAIAFQVERVTGLDFLIQPGDHVDIIFRGEVQVLQPSADS
                     TTAAPRFEPITGLENAPTVKTLLQDKRVLYVSASKIKPVADAAATPVPGSTTKADVPI
                     ETVVVVFAGTDSDAEVIKFAQTDQSILGPLTAVLRATGDTAVEKTTGVTFKILVERFG
                     IPIPNIIQIKLPPTP"
     gene            6139..7359
                     /locus_tag="VIN32_00155"
     CDS             6139..7359
                     /locus_tag="VIN32_00155"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8921023.1"
                     /translation="MIKVLIVDDIPDTREHLSKLLGLERDLDVAGTAGTGEEAIKVAM
                     ELRPDVIVMDINMPGMDGVAAAEIISQRLPTSPIIMMSVHGEATQLKRAISAGAREFL
                     VKPFSGDEFSASIRTVFERELARRSQIEASVPALTAQVNPTDDGEHQVIAVFSPKGGA
                     GRTTIATNLAVALHRETGARVCLVDANLQFGDVGVLLNLNPKDRSIADAVETGDPDGD
                     IVDSCVIDHSTGIRVLLAPPSPEGADLVTAHHMRQIIDHLRIGHEYVVIDLPSGLSDL
                     SLAVMDAADTILVMTALEITTIKNVRLFLEVAEQLEYARTKIRLVINRADAAQGIRIA
                     DVEASVRRPIDGAIVSDGRLAVLAVNRGVPFVVSHPDSPLSRDVTKLARTLAGESAAV
                     VQDQTNKRGLFARR"
     gene            7378..8811
                     /locus_tag="VIN32_00160"
     CDS             7378..8811
                     /locus_tag="VIN32_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HEY8921024.1"
                     /translation="MSLLKRIERARPGGEGEPQVPTAPQTTPVPSSGGQPAPPPSGTG
                     GGAASRPFLGTMPARESFREAKYRVQNRLINELDPKLDLSNQVEVRRQIEELFGKVAD
                     EEGLALTRAERVRMLEQITDEILGLGPLEPLLRDETITEVMVNGPQQVYIEREGRLEM
                     TNVTFQNDEHVMKIIQRIIAPIGRRIDESSPMVDARLADGSRVNCIIPPLSLVGPVLT
                     IRKFAATPFTVEDLIRFGTATPDMFEFLEACVKARLNIFVSGGTGSGKTTMLNILSSF
                     IPDDERIVTIEDAAELQLRQEHVVTLEARPSNIEGKGAVPIRELVRNALRMRPDRIVV
                     GECRSGEALDMLQAMNTGHDGSMSTGHANTPRDMLSRLETMVLMAGMDLPLKAIREQI
                     ASAVDLIVHQNRLKDGTRKIVNITEVQGMEGDVIVMQDIFVFEQTAVVEGKIEGRLRP
                     TGIRPKFVEKFEVAGIHLPPNVFGSPF"
     gene            8834..9820
                     /locus_tag="VIN32_00165"
     CDS             8834..9820
                     /locus_tag="VIN32_00165"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEY8921025.1"
                     /translation="MLPFLIAGLAAGAILVMAVGLAMSGGSGGVAARLERYARSSSAD
                     AAADDPDRDSVVIAGLSRVIEGQDLGTRLSTEIARADLKLRPAEFLFAWMASPFVFAF
                     AAYVIGFIVPGFHNIIALAAVFAIGAYFPRWYIGHRQRKRLAAFSEQLPNTIMLLANS
                     LRAGSSFLQGVELVTREAQPPISEEFERVVREMSLGVALQPALSNLVRRVKSEDLELM
                     VTAINIQSQVGGNLATVLDSIAFTIRERIRIIGEIRVLTSMQRYSGYVITLLPVGLGG
                     LLFLISPTYITALFRNPPVTLGLPTGVIFLIVGLISMGIGYFFIRRIVDIKV"
     gene            9826..10746
                     /locus_tag="VIN32_00170"
     CDS             9826..10746
                     /locus_tag="VIN32_00170"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEY8921026.1"
                     /translation="MPPIVLLIAALAAIAVLVLTWGLSAGSSSRNVVQQRLDQLVVQP
                     KSLEEMELQQPLGERVLRPLVQRLSRLANRGDSGGVIARADAKLERAGYPGGLRGADW
                     MGVKILAMIVLAVVFGFLGLLIGKAPLAFLFGVAGAGLGYMAPEFWLGGRAKKRSFAM
                     VLQLPDALDLLTISVEAGLGFDAALAKVVEKMEGPLVNEFRQALAEIRMGRSRRDALR
                     DVVARADAQPISNFIGAIVQAEQLGVPIAKVLQIQSQQLRIERRQRAEEMAAKAPVKM
                     LFPMVGCIFPTIFIVILGPAIITVMSGPGI"
     gene            10748..11467
                     /locus_tag="VIN32_00175"
     CDS             10748..11467
                     /locus_tag="VIN32_00175"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY8921027.1"
                     /translation="MQPALRPALLLVDDDPTLLSVLARRMAREGYEVLTAPSGAQALT
                     QLEQRWPSLLIVDLMMPGMDGFELCARVKRIADLPIIVLSAVDASEAKVRALEDYAED
                     YITKPFDPDELVARVQRVLKRSAAGHSQVVLDGGDLQIDLTGRRVTRPGGTFPLTPTE
                     VRLLHVLIAQMDRTVPTDTILNRVWSESDGADPSYVWVTIRRLRRKVEVDPDRPRFLV
                     TVRGVGYRLVSFTAADRAAAE"
BASE COUNT         1854 a         4033 c         3740 g         2014 t
ORIGIN      
        1 gtgggccaac ctgcacgccg gctgggtcct cctcttcctg ttcggagcgg ccgtgctggt
       61 cggcgaggtc gtggaccgag cgctgcgacg cgaaccggcc ggaaagccgg ccatgtcatg
      121 gggccagatc cgcgacctgg cggtggcact gctcgtcagt ctcgcggccc tcgcgctcaa
      181 cccgaacggc gtggcgctct ttgcctaccc gttcgacacg gtgggaatca ccgcgctgaa
      241 gcggtatgtg atggagtggt tcccggcaga cctcggcagc atcttcggct ggctgctgct
      301 cggattcgtg gtggtcgggg tcgccccgac attcgccttc gcgcgccatc ggctccgaac
      361 ctcggatgcg ctgatcctgg tggggcttac cgtgatggcc tggcaggcaa tccgcttcct
      421 gctcatcatc ggaccgattg gtggcgcgat cgtggcagtc gtgctctcgc cggtcatcgc
      481 ccagtcgagg gtgggcaggg gaatgtccgg gatcctcgcg cgactcgcgc gaccggtggg
      541 tggcaggcgc ggtgccctca acgcggggat cgcggccatc ctgctggtac tgggcatcgg
      601 cgtcgcgctc gtgcgtacca tgccgaccgc ccaggcagca gagatcgccc gcgggctgcc
      661 tgtggacgcg gtcgcctgga tggacgcgaa tgatccgggt acgcgcatgt tcaaccgata
      721 cgagtggggc ggctacatcg gccagcatcg gcccgaccag ttgatcttca tggacgggcg
      781 ggcggacctg tacggcgacg agctgctcca gatgtacgtg gcgatcatcg gcctccacgg
      841 cgatccgcag gtcaccttcg accgctacga gatcgactac gccgtctttc ctccggacac
      901 gcccctcgcc gactggttcg attcctccgc cgcgtggaag cgggtctacc tcgaccaggc
      961 ggccgccatc tgggttcgcc gctgatgcgt cgcgacctgg tcatcctctt cgtgctggcg
     1021 ctcgtcaccc ggctcctcgc cgccctgctg gtcgactacc cgccctacgg tgaccccgcc
     1081 tactaccaga tgatcgcgga gcagctcgca gctgggcacg gtttcagcgc gcccgtgctg
     1141 tggagcttcc tggaagtggg tggccgcctg ccggttgacc cgatgctccc catccccagc
     1201 aacgggcact ggatgccgct gacctcgatc gtcgccgccg gctccatggc cgtcttcggc
     1261 gacctcctgg gctcgtatcg cgccgcacag ctgccgatga tcctgctggg agccgtgctc
     1321 gtcccgttca cctattacgt cgcgatcgac ctctggggat cgcgattcag cgcctggatc
     1381 gcggctctgc tggtcctcac cgccgggccg ctgctggtga tggccccact cgtggacgcc
     1441 ttcgtcgtct tcggggccgc tggcagtgcg gccatctggt gctcgattcg cgccgtccgc
     1501 gctggccggc ccggcccgtg gctggtggcc gccggcgcct tcgtcggcct ggccaccctg
     1561 gctcgggtgg atggactgct gctcgcggcc gcacccgctg tcgcgtgggc ggttcaccct
     1621 gaatggccgc caaggtcggg gcgcctcgcc tgggggtttg ccagcttcct ggcagccctg
     1681 gccgtgctgg cgccgtggtt cgctcgcgat ctctcagtct ttggcagccc actcccgtcc
     1741 gccggcggcc acaccctctg gatcaccagc tacaaccagc agttctcgat cgccggcgac
     1801 ccatcgctgg cggagtacct ggcatggggt ccggcgaaca tcatcggctc gaagctggcg
     1861 gcctggggcg agctggtggg acggaccgcg gtcctgctgg gcgggatctt catcctgccg
     1921 ttcgccttcg gcctgtggcg cgagcgacac cgacgggagc tgcagccgtt ccttgcctac
     1981 ttcgtggtca tgttcctggc catgggagcg gtcttcacct tccacgcccc gaagggtgcg
     2041 ttctaccact cgtccctcgc ctggctcccg ttcggcgccc cgctcgcggt cgcaagcctg
     2101 gcacccatgg ccacggccgc cggacgggca tggccgttcc tgcggcgccc cgccacgcac
     2161 cgctttctcg ccgtcgcagg cctgatcggg gcgatcgtcc tctcgctggt cggctcggcg
     2221 gtgctcctcg ctcagtggtc ggacacccac gcacgacttc gccaggcggc gcagttcctg
     2281 gcggaccgcg acgcccaagc ccaggtcgtc atggcatacg accccgcggc gctccacgca
     2341 ctgagcggca accctggcgt ggcgcctccc ttcgacctct ttccggtcat cggccaggtg
     2401 gcgggcgcct acgacgtgcg ttgggtggtc gtcaccctgg caccgggcga aacccgggat
     2461 ccgctgggcc tgtgggatgg cgcggcggcg accgatgcag cagggaacca cccggacttc
     2521 ctgccggccg cccctgactt cgactcgccc ggggttcgcg tctttcgggt cgtggacgag
     2581 gcgccgcccc cctcggacta gtacttccgg cgcccgaagt actgttgacc cgatgcacct
     2641 ttggccggga gcatgaccgc tgggtcgggt actgcgccgg gccccgtacc aacgaggacc
     2701 agcgacttgc caaggaccgc gcgcccccga acgccgtcga acgctcccat tcccctgccg
     2761 agcagctttg accagctgcg cgcacaggcc tcggagagcg tcacgcgcaa tgcgtggcgc
     2821 cttcgcgagg tggccgagat ctaccgcgag cagtaccgca cccgcctcga cgagtgggtc
     2881 cagctgcggg cgcgcgtcga tcatcgtgtc gccgccgccc gtggaaccgg cccctcgcca
     2941 tccgtgctgc gtgccgaccg cgatgcgctg ggcagggagc tcgcgcagct ccagtctggg
     3001 ctgaagcgga tcgacatcgc cgctcgccag atggagctcg tcctgaccta cctcagcagc
     3061 gacgacgagc gggcctccga gccgaccctc tcagacgccg atctgtcgcc ggcggccatc
     3121 tcgctgaaga tcatccaggc acaggaggga gagcggcagc ggctcgccga agaggtccac
     3181 gatgggcctg cccaggtcct gaccaacgcc atcttccagg tcgagtacct cgatcgggtg
     3241 atcgacgact cacccgagac cgctcggcat gagctcgcct tcctgcgcac catgctgcgc
     3301 gagggactcg atgaggttcg ctcgttcatc accgcgcttc gacctccatc ggtcgacgtc
     3361 ggattgaagc aggcgatcac cgctgccggg gccgagttcg cggccaagca cgggatcgcc
     3421 gtcgaggtgt cggtgccggg catcgactcc cacgttcctg gcgaggccaa ggcaccgatg
     3481 ctgcggatcg tgcaggaagc cctccagaac gtacgaaagc acgcggccgc cacatcggtc
     3541 cgcatcagcc tcgagcacgg tcacctggtg atcgtcgaca acggtcgggg cttcgacgtg
     3601 atgcggctcg cctcggccag ccgcaacttc ggcctgcagt tcatgcgcga gcgcgctgag
     3661 ctgatgggct cgtcactcca aatcgagtcc cgacaggggg aggggacccg catccttctt
     3721 cgcataccgg aggttcacta atggttacca tggctgccaa ggagaagaag atggtggaac
     3781 aggaaaccga gcgccgtaag ggcgctgacc ggcgcgccgc gggggcaaag gcgcgcatcg
     3841 tcatcgtgga cgggcacacc ctgttccgcc gcggcgtgcg caacattctc gagctcgagg
     3901 acgacatgga ggtcgtcggc gaggccggca acggccgcga ggcgatcacc accatccagg
     3961 agctgacccc cgacgtagtc ctgatggacc tcaccctgcc cacgccaaac gggatcgaga
     4021 cgacccagcg ggtcaagcgt gagctcccgc acaccgccat catcgtcctg gcgccgaacg
     4081 aggacgagga ccagctcttc gaggcgatca aggccggcgc cgcggcctac gtcctgaagg
     4141 acatcgatcc gacggacctg atcgcgatca tccgccgcgt gcgctccggc gagtacctga
     4201 tcaatgacaa ggtcttcagc aagcccgctg tcgccagccg cgtcctcaag gagttccgcg
     4261 agcttgcggt ctacggggcc gatgcccagc cgatcttcgc gccgctctcg ccacgagagg
     4321 tcgagatcct ggacaacatc gctcagggga tgaccaacaa gcaggtcgcc tatgcgcttg
     4381 ggatcagcga gcagacggtc aagaaccata tgtcgagcat cctgcgcaag ctgtcggtca
     4441 atgaccggac ccaggccgtg gtgtacgcca tgcgccaggg ctggatcaag atccccgagg
     4501 actgatcgca aaaggtccca gaaacccccg gtactcctgc gccatgggca ggaacagcgg
     4561 gggcacctag tctgggggcc tgtcgtcgac gggtatccgc ggcgacattc atcctctcct
     4621 ccggcacgag tgccgcgccg cagcagcgcg gcacttgtgc ttcccagggg tggcggggcc
     4681 ttgacaagat gcaggggctg ctgcaaaggt acgcaccggc tgtgggcgca ttgggaccat
     4741 cgggtcatat tcatacccgt cattgcacct ttacgctgcg gcagccttat ctttgaggaa
     4801 aggaggtaac ccacatatga ttcagttcat cagcactttt atcgctcgat tccaccgtga
     4861 ggacgaggaa ggccagactc tcgttgagta cggtctgctc ctggccctca ttgcgatcat
     4921 cgtgatcgtc gccctgctct tcctgggccc gatcgtgagc cagatcttcc agaacgtcgg
     4981 cgacaacctg aacaggtaat tgagcctcgc tcaatatcgc tagacactct gaggcccccg
     5041 ggatccccgg gggcctcagc acgttcaggg gccgaacgcc tctgaggtac caccagctcc
     5101 atagccgata agtccggtcg atcccgatcg tggctggccc gtagactgtt gaccgcagtc
     5161 acaacgtcga acagactgca cggccggatt gtcctcatcc tcgctcgaag ggaaatatcg
     5221 tgaagcgatc aaatcgcctg gtcatcctcg tcggcgtgct gctcgccgtc ctggcgttcg
     5281 tgggcatcgt gattctcctc gggcagccaa aggcagagac ccccaagaag ccgacggtgg
     5341 acgtcctcgt cgcaaagcag gacatcgcca tcggcgattc ggtgactccc gacctcgtcg
     5401 aggtcaagcc ggtcgatcct gctgcggtct ccggcactcg catcgctgat ccctcacagc
     5461 tgagcggcgc cccggccctc ttcccgatcg gcgcgggttc ccaggtcagc caggaggcag
     5521 tcgggctggg aaccaatacg aacatcccgt gcattgtctg ccagctccag cccggcgaga
     5581 aggcaatcgc tttccaggtc gagcgcgtca ccggcctcga cttcctgatc cagccgggtg
     5641 accacgtcga catcatcttc cgaggcgagg tgcaggtgct ccagccgtcc gccgattcca
     5701 cgaccgcggc gccgcgcttc gagccgatca ccggcctgga gaatgcgccg accgtcaaga
     5761 cgctgctgca ggacaagcgc gtcctgtacg tcagcgccag caagatcaag cccgtggccg
     5821 acgctgcggc taccccggtc ccgggctcga ccaccaaggc cgacgttccc atcgagaccg
     5881 tggtcgtcgt cttcgccggc accgattcgg acgcggaggt gatcaagttc gcccagaccg
     5941 atcagagcat cctcggcccc ctcaccgcgg tccttcgggc cactggcgac acggcggtcg
     6001 agaagacgac gggcgtcacc ttcaagatcc tcgtcgagcg cttcggcatc ccgatcccca
     6061 acatcatcca gatcaagctg cctccgaccc cataaggggc ctgggcggcc gccacctcag
     6121 catccagggg aacacaacgt gatcaaggtc ctcatcgtgg acgacatccc ggacacgcgg
     6181 gaacacctct cgaagcttct cggcctcgag cgggacctcg acgtggccgg gacggccggc
     6241 acgggagagg aggcgatcaa ggtcgccatg gagctgcgac ccgacgtgat cgtcatggac
     6301 atcaacatgc cggggatgga tggagtggcg gcggccgaga tcatctcgca acgcctcccg
     6361 accagcccga tcatcatgat gagcgtccat ggggaagcga cgcagctgaa gcgggccatc
     6421 tccgcggggg cccgcgaatt cctcgtcaag cccttctccg gtgacgagtt ctccgccagc
     6481 attcggaccg tcttcgagcg cgagctcgct cggcgaagcc agatcgaggc gtcagtcccg
     6541 gcgctcaccg ctcaggtcaa tccaacggac gacggggagc accaggtgat tgccgtcttc
     6601 tcgcccaagg gcggcgctgg acggaccacg atcgcgacga acctcgcggt cgcgctgcac
     6661 cgtgagacgg gggcgcgcgt ctgcctggtg gatgccaacc tgcagttcgg agatgtcggc
     6721 gttctcctca acctcaaccc caaggaccgg tcgatcgccg atgcggtgga gaccggcgac
     6781 ccggacggag acatcgtcga ctcgtgcgtg atcgaccact cgaccgggat ccgcgtgctg
     6841 cttgctccgc catcccctga aggcgccgat ctcgtgacgg cgcatcacat gcgccagatc
     6901 atcgaccacc tgcgcatcgg ccacgagtac gtcgtgatcg accttccgtc ggggctgagc
     6961 gacctgtccc tggcggtgat ggacgcagcc gacacgatcc tggtgatgac cgccctcgag
     7021 atcaccacga tcaagaacgt aaggctcttc ctcgaagtcg ccgagcagct cgagtacgcg
     7081 cggaccaaga tccggctcgt gatcaaccgc gccgacgccg cacaggggat cagaatcgcc
     7141 gacgtggaag cctccgtccg ccgccccatc gatggggcga tcgtgtcgga cgggcgcctc
     7201 gcggtgctcg cggtgaatcg tggcgttccc ttcgtcgtct ctcatcccga cagcccgctc
     7261 tcgcgtgatg tcaccaagct cgcacgaacc cttgccggcg aaagcgccgc cgttgtccag
     7321 gaccagacca acaagcgcgg attgtttgcg cgccgatagc gcggaaggac tgaccagatg
     7381 tcattgctga agaggatcga gcgagcgcgc ccaggcggtg aaggggagcc gcaggttccg
     7441 accgccccgc agacgacacc ggttccgtcg tcgggcggac agccggcccc gccgcccagc
     7501 ggcacgggcg gtggcgcggc gtcgcgcccc ttccttggga caatgcctgc ccgcgagtcg
     7561 ttccgggagg cgaagtatcg cgtccagaac cggctcatca acgagctcga tcccaagctc
     7621 gacctgagca accaggtcga ggtccgtcgc cagatcgagg agctcttcgg caaggtcgcc
     7681 gacgaggagg gcctggccct cacccgcgcc gagcgggtgc gcatgctgga gcagatcacc
     7741 gatgagatcc tgggcctcgg accgctcgag ccgctccttc gcgacgagac catcaccgag
     7801 gtgatggtca atgggccgca gcaggtctac atcgagcgtg agggtcgcct ggagatgacg
     7861 aatgtcacct tccagaacga cgagcacgtg atgaagatca tccagcgcat catcgccccc
     7921 atcggccggc gcattgacga atcgagcccg atggttgacg ctcgactggc cgatggctcg
     7981 cgcgtcaact gcatcatccc gccgctgtca ctggtcggtc cggtcctcac catccggaag
     8041 ttcgccgcca cgccgttcac ggtcgaggac ctgatccgct ttggcactgc gacgcccgac
     8101 atgttcgagt tcctcgaggc ctgcgtgaag gcacggctca acatcttcgt gtccggaggc
     8161 accggctcgg gcaagacgac catgctcaac atcctctcct cgttcatccc cgatgacgag
     8221 cggatcgtga ccatcgagga cgccgccgag ctccagctgc gacaggagca cgtcgtgacg
     8281 ctcgaggcac gtccatcgaa catcgagggc aagggcgctg tcccgatccg cgagctggtg
     8341 cgcaacgccc tgcgcatgcg gcccgaccgc atcgtggtcg gagagtgccg ctcgggcgag
     8401 gcgctcgaca tgctccaggc catgaacacg ggccacgacg gctcgatgtc gaccggccac
     8461 gccaacaccc cgcgggacat gctgtcccgt ctcgagacga tggtcctcat ggccggcatg
     8521 gacctgccac tcaaggccat ccgcgagcag atcgcatcgg ccgtggacct catcgtccac
     8581 cagaaccgcc tcaaggatgg aacgcgcaag atcgtgaaca tcaccgaggt ccaggggatg
     8641 gagggggacg tgatcgtcat gcaggacatc ttcgtcttcg agcagaccgc cgtcgtcgag
     8701 ggcaagatcg agggtcggct gcggccgacc gggatccgtc ccaagttcgt cgagaagttc
     8761 gaagtggccg ggatccacct gccgccgaac gtcttcggct cgccgttcta gcaggattca
     8821 ggagtccatt ggcatgttgc cgttcctgat cgcgggtctc gctgcaggcg ccatcctggt
     8881 gatggcggtt ggcctcgcca tgagcggggg atccggtggt gtggccgctc gcctcgagcg
     8941 ctacgcccgc tcctcctcgg cggatgccgc ggcggacgac ccggatcgcg actccgtggt
     9001 gatcgccgga ttgagccgcg tgatcgaagg gcaggatctc gggactcgcc tctcgaccga
     9061 gatcgctcgc gccgacctga agctgcgtcc ggccgagttc ctcttcgcct ggatggcctc
     9121 ccccttcgtg tttgccttcg cggcctacgt catcgggttc atcgttcccg gattccacaa
     9181 catcatcgcc cttgcagccg tgttcgcgat cggcgcgtac ttcccgcgct ggtacatcgg
     9241 gcacaggcag cgcaagcgcc tggctgcgtt cagcgagcag ctgccgaaca ccatcatgct
     9301 gctcgccaac tcgctccgcg ccggctcgtc cttccttcag ggcgtggagc tggtcacgcg
     9361 cgaggcgcag ccaccgatct cggaggagtt cgaacgcgtc gtgcgggaga tgagccttgg
     9421 cgtcgctctg cagcccgcac tctccaacct cgtgcgccgc gtcaagtcgg aggacctgga
     9481 gctgatggtc accgccatca acatccagag ccaggtcggt gggaaccttg cgacggtcct
     9541 cgactcgatc gccttcacca tccgcgagcg gatccggatc atcggtgaga tcagggtgtt
     9601 gacctcgatg cagcgctaca gcgggtacgt catcaccctg ctgccggttg ggctgggagg
     9661 gctcctgttc ctgatcagcc ctacctacat caccgcgctg ttccgcaatc cgccggttac
     9721 gctcggcctg cccaccggcg tgatcttcct gatcgtcggg ctgatcagca tgggcatcgg
     9781 ctatttcttc atccgtcgca tcgtcgacat taaggtctga tacccatgcc accgatcgtt
     9841 ctcctcatcg cagcgcttgc cgccattgca gtgctcgtcc tgacttgggg cctttccgca
     9901 gggtcgtcat cacgcaacgt ggtccagcag cggctcgatc agctcgtcgt gcagcccaag
     9961 agcctcgagg agatggagct ccagcagccg ctcggcgagc gagtgctgcg cccgctcgtt
    10021 cagcgcctgt cgcgactcgc caaccgcggc gactccggag gggtgatcgc ccgtgccgac
    10081 gccaagctgg agcgggccgg gtatccgggt ggcctgcgcg gtgccgactg gatgggcgtc
    10141 aagatcctgg cgatgatcgt cctcgccgtc gtcttcggat tcctggggct gctgatcggc
    10201 aaggccccgc tggcgttcct gttcggggtc gccggcgcgg ggctcggcta catggcgccc
    10261 gagttctggc tcggtggacg cgcgaagaag cgctcatttg ccatggtcct gcagctgccc
    10321 gacgccctcg acctgctgac catctcggtc gaggccggcc tcggcttcga cgccgcactc
    10381 gccaaggtgg tggagaagat ggaggggccg ctggtcaacg agttccgcca ggcgctggct
    10441 gagatccgca tgggccgaag ccgcagggac gcgctgcgcg acgtcgtcgc gcgggccgat
    10501 gcgcagccaa tcagcaactt catcggcgcc atcgtgcagg ccgagcagct cggcgtgccg
    10561 atcgccaagg tcctccagat ccagtcgcag caactccgca tcgaacgacg tcagcgcgcg
    10621 gaggagatgg cggccaaggc cccggtcaag atgctcttcc cgatggtcgg ctgcatcttc
    10681 cccacgatct tcatcgtcat cctgggtccg gcaatcatca cggtcatgtc gggacccggc
    10741 atctgacatg cagccggcgc tgcggccggc cctcctgctg gtcgatgacg accccacgct
    10801 cctctccgtc cttgcgcgtc gaatggcgcg cgagggttac gaggtcctga ccgccccctc
    10861 gggtgcccag gcgctcacgc agctcgagca gcgctggccg tccctgttga tcgtcgacct
    10921 gatgatgccc ggcatggacg ggtttgaact gtgcgcgcgg gtgaagcgga tcgccgacct
    10981 gccgatcatc gtgctctcgg cggttgatgc atccgaggcg aaggtccgcg ccctggagga
    11041 ctacgccgag gactacatca cgaagccctt cgaccctgac gagctggtcg cgcgggtcca
    11101 gcgcgtcctg aagcgatccg cagccgggca ctcgcaggtc gtgctcgacg gcggcgacct
    11161 ccagatcgac cttaccgggc gtcgcgtcac ccgcccaggc ggcacgtttc cactgacgcc
    11221 gaccgaggtg cggctcctgc atgtgctgat cgcgcagatg gatcggaccg tcccaaccga
    11281 caccatcctg aaccgcgtct ggtccgaatc ggacggagcc gatccgtcgt acgtctgggt
    11341 caccattcgc cgcctgcggc gcaaggtcga ggtggatccg gaccgcccgc gattcctggt
    11401 caccgtgcgc ggggtcgggt accggctcgt ctccttcacg gcagccgacc gcgcggcagc
    11461 ggaatagcgc cgtggggatc ggccgcccat ccgtacgcct cctggcggtg ctggtcgtcg
    11521 ctgccctgcc gccgctgatc gcctacggcc tgctgaccgt cgccgagccg agttgggtgt
    11581 cacgggtagg cgctccgcta tcgatgctga tcctggttgg cgccgggctg ttgtggatcg
    11641 c
//
LOCUS       DATCQR010000006         2842 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_77701, whole genome shotgun sequence.
ACCESSION   DATCQR010000006 DATCQR010000000
VERSION     DATCQR010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2842
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77701"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="VIN32_00180"
     CDS             <1..244
                     /locus_tag="VIN32_00180"
                     /inference="COORDINATES: protein motif:HMM:NF025052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY8921028.1"
                     /translation="AVARRLGRPAAAIVFNLPLATCLERNAARTDRRLPPAAIRRQHA
                     RMRASLDGLGAEGFDPIVVLSTIEAVEATTVAPGDS"
     gene            complement(241..1116)
                     /locus_tag="VIN32_00185"
     CDS             complement(241..1116)
                     /locus_tag="VIN32_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921029.1"
                     /translation="MSDPRWAVQLDPPVLLPGRSVSVNLTYTPDRDHDGRAVRARLRC
                     RERYRYSRTEGTGSTAHRVTRTGVEELARIEAEIAGGQRFVRGQPVSWHVSFDVPELG
                     PATFEGDVLRCDWTLEANIDLPMGLDPRVEETVHVAQPMALLRAGVIDTGQFGLFETA
                     PVNVDALPAQIRLKPVPVSLQSPFEGAFTVEAGVGTVQEVRLELRLVSEVTVRGGHHD
                     EIVAWRGVLAGPRQFGGALAEHPFTAEATGEWLPSVDLPHGRARGQFHVILAQEWAPD
                     IHYVRDVALATTTEL"
     gene            1416..1835
                     /locus_tag="VIN32_00190"
     CDS             1416..1835
                     /locus_tag="VIN32_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012772234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921030.1"
                     /translation="MNASRATGSGTKADPWKLRTPTGTSEYEMYRDETLDPPAIVCQV
                     GTTQLRYDLRAIDDLHAMLKAHGDWMPLGSADEQKEATDGTVEAWGRSPSNPVGGWYG
                     LKKGLRGRFGMYMPPLLEALGLAEVEHNPKNNRMRAL"
     gene            1847..2413
                     /locus_tag="VIN32_00195"
     CDS             1847..2413
                     /locus_tag="VIN32_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012998528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY8921031.1"
                     /translation="MAAAHHLLHAGYARDDGVASSVSLVIDGAAVIVVDPGMVADPEL
                     ILAPLRGFGLAPDHVTHVVITHHHPDHTVNIGLFSNAEVVDFWARYRGDQWLDHDGDG
                     YRVSDHARLILTPGHTSEDATLLVETDAGVVACTHAWWRGDRTPEVDPLADDQQALEA
                     SRKRILAEADMIVPGHGEPFASRRRPPG"
     gene            complement(2417..>2842)
                     /locus_tag="VIN32_00200"
     CDS             complement(2417..>2842)
                     /locus_tag="VIN32_00200"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY8921032.1"
                     /translation="RLILELTESVLLDREGAPMTSLATLHELGIRLAIDDFGTGYSSL
                     SYLNDLPIDILKIDISFVAALDAGREAPAVVRSIIRLAGTLGLHTIAEGIERPEQAGR
                     LRTLGADYGQGFHFAPPLNAEDLAAYLRDRAWSPAAAEA"
BASE COUNT          488 a          958 c          970 g          426 t
ORIGIN      
        1 ggccgtagcg cggaggctcg gacgccctgc cgccgccatc gtcttcaacc tgccgctcgc
       61 gacatgcctc gagcggaacg cggctcggac cgatcggcgc ctcccacccg ccgccattcg
      121 acggcagcac gcgcgcatgc gcgcctcgct cgatggcctg ggggccgagg gtttcgatcc
      181 gatcgtcgtg ctgagcacaa tcgaggccgt cgaggccaca acagtggcgc caggcgactc
      241 ctagagctcg gtggtggtgg cgagcgccac gtcacggacg tagtgaatgt ccggcgccca
      301 ctcctgggcc aggatgacgt gaaactgacc gcgcgcccgg ccgtggggca ggtcgacgga
      361 gggcagccac tcaccggtgg cctcagccgt gaagggatgc tcagccagcg ctccaccgaa
      421 ctgcctggga cccgcgagca cgccgcgcca cgccacgatc tcatcgtggt gcccgccacg
      481 cacggtgacc tccgacacca gccgcagctc caggcgcacc tcctgaactg tgccgacccc
      541 tgcctcaacg gtgaaggcgc cttcgaacgg tgactgcagg gagaccggaa cgggtttcag
      601 ccgaatctgc gccgggaggg cgtccacgtt gaccggtgca gtctcgaaca ggccgaactg
      661 gccggtgtca atcacgccgg cgcggaggag cgccatcggc tgagcgacgt ggacagtctc
      721 ctcaacgcgc ggatcgagcc ccatcggcag atcgatattc gcctccagcg tccagtcgca
      781 gcgcaggaca tcgccctcga aggttgccgg cccgagctcg ggaacgtcga agctgacatg
      841 ccagctcacc ggttgcccgc gcacgaatcg ctggccgcct gcaatctcgg cctcgatacg
      901 cgccagctcc tcgacgccgg tgcgcgtgac acggtgggct gtgctgccgg taccctcggt
      961 tcggctatag cggtagcgtt cccggcagcg gagtcgcgca cgcacggcac ggccgtcgtg
     1021 atcccgatcg ggcgtatagg tcaggttgac gctcacgctg cgccccggca ggagcactgg
     1081 cgggtcgagc tgcactgccc accgtgggtc actcaccggc gcggatcctc gttgaggcgg
     1141 gcaacgaagt gtcccgaagg aactgcgccc ggcgccgcag tgcgagactg ccacaccggt
     1201 accggaacgc ttgatgcaat cgcatgatcc ggtatcgcgg ccccttcggg acgaccgaag
     1261 gatagcccgc cctcaccggg gacaacaacc ccatttatcc ccgacttttc gagaccgatt
     1321 cgtccttttc cacagggctt ccccaaccgg ttcctggtca ttggcggtcg tcgtcgcggg
     1381 tagaatcgtc gtcccacgcc gctggagatc attcgatgaa cgcgagccga gccacgggat
     1441 cgggcaccaa ggccgatcca tggaagctgc ggaccccgac agggacctcc gagtacgaga
     1501 tgtatcgcga cgagacgctc gatccgccag cgatcgtgtg ccaggttggc accacccagc
     1561 tgcgctacga cctccgcgcg atcgacgacc tgcacgcgat gctgaaggcc cacggcgact
     1621 ggatgccgct cggcagcgcc gacgagcaga aagaggcgac cgacggaacg gtcgaagcat
     1681 ggggccgatc ccccagcaat cccgttggag gctggtacgg cctgaagaag gggctccgtg
     1741 gccgattcgg gatgtacatg ccgcccctgc tcgaggcgct gggtctcgcc gaggtggagc
     1801 acaaccccaa gaacaaccgg atgcgggcgc tctaggcctg caccggatgg ccgctgcaca
     1861 tcaccttctc cacgccggct acgcccgtga cgatggggtg gcgagcagcg tcagcctggt
     1921 gatcgacggt gcggcggtga tcgtggtcga cccggggatg gttgccgatc ccgagctgat
     1981 cctggccccg ctccgagggt tcgggctggc acccgaccac gtgactcacg tggtcatcac
     2041 gcaccatcat cccgatcaca cggtcaacat cggacttttc tcaaatgccg aggtcgtgga
     2101 cttctgggcg cgctaccggg gcgaccagtg gctcgaccat gacggcgacg gatatcgggt
     2161 cagcgaccac gcccggctga tcctgacccc gggtcacacg agcgaggatg cgacgctcct
     2221 ggtcgagacc gatgcgggcg tggttgcctg cacgcacgcc tggtggcgag gcgaccggac
     2281 gccggaggtc gatccgctgg ccgatgacca gcaggcgctg gaagcgagcc ggaagcggat
     2341 cctggccgag gcagacatga tcgtccccgg acatggagag ccgttcgcca gcagacgccg
     2401 gccaccgggc tgaagttcag gcttcggcgg cggctgggga ccaggcccgg tcgcgcaggt
     2461 aggcggcgag atcctcggca ttgagcggcg gtgcgaagtg gaatccctgg ccgtagtcgg
     2521 ctccgagcgt ccgcagcctg ccggcctgtt cggggcgttc gataccctcg gcgatggtgt
     2581 gcaggccaag cgtgccggcg aggcggatga tggagcgaac gacggccggc gcctcacgcc
     2641 cggcgtcgag cgcggccacg aacgagatgt cgatcttcag gatgtcgatc gggaggtcgt
     2701 tcaggtagct gagggacgag tagccggtcc caaagtcgtc gatggccagt cggatcccca
     2761 gctcgtgcag cgtggccagc gaggtcatcg gcgctccctc gcggtccagc aggacggatt
     2821 cggtcagctc gaggatgaga cg
//
LOCUS       DATCQR010000007         6246 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_139835, whole genome shotgun sequence.
ACCESSION   DATCQR010000007 DATCQR010000000
VERSION     DATCQR010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6246
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139835"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2143
                     /locus_tag="VIN32_00205"
     CDS             <1..2143
                     /locus_tag="VIN32_00205"
                     /inference="COORDINATES: protein motif:HMM:NF012731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEY8921033.1"
                     /translation="RLTAISDEMLADIEKETVDFVDNYDGRLREPTVLPARLPNLLIN
                     GSSGIAVGMATNIPPHNLTEICLAVIALIDNPDMTVDDLCDIVTGPDFPTGGTIFRVE
                     DQRNSLTGEKERVDVIRHMYATGRGRVVIRGQVAFEEDARGRISVVITELPYQVNKTT
                     LIEKMAELVSGKRITDVSDIRDESDRTGMRIVVEIKRDGSPHTVMQQLFKHTALQTSF
                     SANMLALVDGQPQTLGLKRMLEHYITWRREIVRRRTEFDLARAKERAHILEGLKIALD
                     NLDEVIKTIRASADTDAARAALVKRFKLSEAQANAILEMQLRRLAALERKKIEDEYES
                     VIRLIAELEDLLANPRKVLTAIRDELDELIRKYGNDRKTRIESDANRELTAEDLVAAE
                     DVVVTLSQRGYIKRQQIGTFRSQRRGGKGKLAMITREEDAVRHLMVANTHDNILFFTN
                     RGRVFITKVHTLPEASRQAKGLPIINLPGVMVDQGEYVSAIITIPQFEADHYMAMATR
                     GGMIKKTSLAEYAKIRSNGLIAISLVEGDELRWVGLTDGQNDIILATRNGQAARFHET
                     EVRPMGRDTRGVTGIRLRGDDEVVGMEVVAPSAELLVVTEGGYGKRTVLDDFPVKHRA
                     TGGVIANSLNAETGKVAAVRVVGREDEELMLITEEGTILRTEVSSVNRYRRASRGVTV
                     MKPGEGDRIVSIAVFVDDRPPIAEDDAETEG"
     gene            2202..3236
                     /locus_tag="VIN32_00210"
     CDS             2202..3236
                     /locus_tag="VIN32_00210"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEY8921034.1"
                     /translation="MYADEFSIYAGNANRPLAEQICRYLRTELGKAEVFKFANDNIFV
                     RVMENVREQDVFLVQPTSRPVNDSVMELLIMIDAFKRASAGRITAVMPYYAYGRSDKK
                     DQPRVPITARLVADMLTVAGADRVLTMDLHQGQIQGFFSIPVDELTAVNILSRYIRDK
                     NLDDIVVVSELGFAKKARNFAEILEAPLAIVEKRRVGNEDKTELMNIIGEVAGKTAVI
                     IDDEIDTAGSLLEAVAALVAEGAVAIYSCATHGVFSNRSLEHIQASPLTEVIVTDTIP
                     LSADANRDKVTVLSIAPLFGEAIKRIHRGESVGALFSSEVQLVEEMTFWGSHAADEEA
                     EEPPAAAAEG"
     gene            3285..6158
                     /gene="secA"
                     /locus_tag="VIN32_00215"
     CDS             3285..6158
                     /gene="secA"
                     /locus_tag="VIN32_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014435108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEY8921035.1"
                     /translation="MLKFLSRLTDSNDREVRRFEPTVARINELEPEYASLTDTDLRGK
                     TAQFRARLRDSLGDLLIPIEQRALTPEQAEESELVASDPMRISEERKEQHKRERAQID
                     AALDEILPEAFAAVREAMRRALAKRHYDVQLVGGMVLHSGAIAEMKTGEGKTFVAPLA
                     AYLNGLTGRGVHVITVNDYLAKRDAQWIGQVFWRLGITVGSIQHEAAYLVDPDFPQTD
                     EHLQALRPVARAEAYAADVTYGTNNEFGFDYLRDNLVVDLGQRVQRGHFFAIVDEVDN
                     ILIDEARTPLIISGAAEESADKYVQFARLVPRLKAEEDYILDEKFRQVAISEAGTDKM
                     ERWLGVENLFDDDFSMARHLEQALKAEALYKRDRDYVVKDDEVVIVDEFTGRLMPGRR
                     WSEGLHQAVEAKEGVKIQNESRTLATITFQNYFRMYDKLAGMTGTAETEAEEFHKIYD
                     LEVVAVPTNQPMIRDDFADLVFASQKGKWASVVDEIVEEHERGRPVLVGTISVEVSEM
                     LGEMLKRRGIRHSVLNAKFHEKEAEIVAQAGHSDSVTIATNMAGRGTDILLGGNPEML
                     AAELLHKKGTNVLEASPEQYAAALAEADRLCAEDRERVIAAGGLHIVGTERHEARRID
                     NQLRGRAGRQGDPGSTRFYLSLEDDLMRRFASDRVQSIMRTLGFNDETALESRMVSKT
                     IEGAQTRVEGFNFDTRKHVVEYDDVINRQRETIYHERERILRSDTLAPTVLAMLEEEV
                     RALVIEHTAGEFAAEWNRDGLKAQLTAMVPTLDPAALAGIDDLVEADQMALLLVELVE
                     DTYQRKRTEVGDEGMLVLERVVLLRVIDSLWVEHLTAVDDMRRGIGLRAYSQRDPLNE
                     FKVEAYRMFDELKSTIRHDITHTVFRVTLTREAPHRHERPMIESRPDVTGAAVTAGAA
                     TAAVGGGNGSRLGPQTAKAGPKLGRNDPCWCGSGKKYKRCHGA"
BASE COUNT         1214 a         2073 c         2087 g          872 t
ORIGIN      
        1 gcgcctgacc gcgatcagcg acgagatgct ggccgacatc gagaaggaga ccgtcgactt
       61 cgtcgacaac tacgacggcc ggctgcgaga gccgacggtg ctgcccgctc gcctgcccaa
      121 cctgctcatc aacgggagct ctgggatcgc ggtgggcatg gcgaccaaca tcccaccgca
      181 caacctgacc gagatctgcc tggcggtgat cgcgctgatc gacaacccgg acatgaccgt
      241 cgacgacctg tgcgacatcg tgaccggccc ggacttcccc actggcggca cgatcttccg
      301 tgtcgaggat cagcgcaaca gcctcaccgg cgagaaggag cgggtcgacg tgatccgcca
      361 catgtatgcc accgggcgcg gacgcgtcgt gatccgcggc caggtcgcct tcgaggagga
      421 cgcccggggc cggatctcgg tcgtcatcac cgagctgccc taccaggtca acaagaccac
      481 gctcatagag aagatggccg agctggtcag cgggaagcgg atcaccgatg taagcgacat
      541 tcgcgacgaa agcgacagga cggggatgcg gatcgtcgtg gagatcaagc gcgacggatc
      601 gccgcacacc gtgatgcagc agctgttcaa gcacacggca ctgcagacca gcttcagcgc
      661 caacatgctg gcgttggtcg acgggcagcc gcagacgctt ggcctcaaac ggatgctcga
      721 gcactacatc acctggcgcc gcgagatcgt tcggcgacga accgagttcg acctggcgcg
      781 tgcaaaggag cgcgcgcaca tcctcgaggg cctgaagatc gcgctcgaca acctcgacga
      841 ggtgatcaag acgatccgcg cctcggcgga taccgacgca gcccgcgccg ccctcgtgaa
      901 gcgcttcaag ctcagcgagg cccaggccaa cgcgatcctc gagatgcagc ttcgccggct
      961 ggccgcactc gagcgcaaga agatcgagga cgaatacgag agcgtcatcc ggctcattgc
     1021 cgagcttgag gacctgctgg ccaacccgcg caaggtcctg accgccatcc gcgacgagct
     1081 ggacgagctg atccggaagt acgggaacga ccgaaagacc cggatcgagt cggacgccaa
     1141 cagggagctg accgccgagg acctggtggc cgcggaggac gtcgtcgtaa ccctctccca
     1201 gcgcggctac atcaagcgcc agcagatcgg caccttccgc tcccagcggc ggggcggcaa
     1261 ggggaagctg gccatgatca cgcgcgaaga ggatgcggtg cggcacctca tggtggccaa
     1321 cacccacgac aacatcctgt tcttcaccaa ccgcgggcgg gtcttcatca ccaaggtgca
     1381 caccctgccc gaggccagcc gccaggcgaa gggcctgccg atcatcaacc tccccggcgt
     1441 gatggtcgac cagggcgagt acgtcagcgc gatcatcacc atcccgcagt tcgaggcgga
     1501 ccactacatg gcaatggcca cccgtggcgg catgatcaag aagacctccc tggccgagta
     1561 cgccaagatc cgcagcaacg gtctgatcgc gatcagcctg gtcgaggggg acgagctgcg
     1621 atgggtcggc ctcaccgacg ggcagaacga catcatcctc gccacgcgca acggacaggc
     1681 ggcccgcttc cacgagaccg aggtcaggcc gatgggccgc gacacccggg gcgtgaccgg
     1741 gattcggctg cgtggcgacg atgaggtggt cgggatggag gtcgtggcgc cgtccgcaga
     1801 gctgctggtg gtcaccgagg gaggctacgg caagcggacg gtgctcgacg acttcccggt
     1861 caagcaccgg gccaccggtg gtgtgatcgc caactcgctg aacgccgaga cgggaaaggt
     1921 ggcggcggtg cgcgtcgtgg gccgcgagga cgaggagctg atgctgatca ccgaggaggg
     1981 gaccatcctg cgcaccgagg tcagcagcgt aaatcgctac cggcgcgcgt cccgcggggt
     2041 gaccgtcatg aagccgggcg aaggggaccg gatcgtgtcg atcgcggtat tcgtggacga
     2101 tcgaccgccg atcgccgagg acgacgcgga aaccgagggc tgagaaaggc cgcggagcgg
     2161 ctagaatcgg ctccgggaga ccaaccgcac cggagacccg cgtgtacgcc gacgagttca
     2221 gcatctacgc cgggaatgcc aacaggccgc tcgccgagca gatctgccgc tacctccgca
     2281 cggagctggg caaggccgag gtcttcaagt tcgccaacga caacatcttc gtgcgcgtca
     2341 tggagaacgt ccgcgagcag gacgtattcc tggtgcagcc gacatccagg ccggtcaacg
     2401 actccgtcat ggagctgctg atcatgatcg acgccttcaa gcgcgccagc gccgggcgga
     2461 tcactgccgt gatgccgtac tacgcatacg gccgctccga caagaaggac cagccacggg
     2521 tccccatcac cgctcggctc gtcgccgaca tgctgaccgt ggcgggggcg gaccgggtcc
     2581 tgaccatgga cctccaccag gggcagatcc agggattctt cagcatccca gtcgacgagc
     2641 tgaccgccgt caatatcctg tcgcgctaca tccgcgacaa gaacctggac gacatcgtgg
     2701 tggtcagcga gctcgggttc gccaaaaagg cgcgcaactt cgccgagatc ctcgaggccc
     2761 cgctggcgat cgtcgagaag cgacgggtcg gcaacgagga caagaccgag ctgatgaaca
     2821 tcatcggcga ggtagccggc aagaccgccg tgatcatcga tgacgagatc gacaccgcgg
     2881 gctcgctgct ggaggcggtc gccgccctcg tggcggaggg ggctgtcgcc atctactcat
     2941 gcgcaaccca cggcgtcttc tcgaacaggt cgctggaaca catccaggcc agcccgctca
     3001 ccgaggtgat cgtcaccgac acgatcccgc tgtccgccga tgcgaaccgt gacaaggtca
     3061 ccgtcctctc gatcgcgccg ctcttcgggg aggcgatcaa gcgcatccac cggggtgaga
     3121 gcgtgggcgc cctcttctcg tccgaggtcc agctggtcga ggagatgacc ttctggggca
     3181 gccacgctgc cgacgaggaa gcggaagaac cgccggcggc cgcggccgag ggctgaccgc
     3241 cgatcgggcc tcatcggccc atccggatat actcgatctt ccagatgctg aagttcctct
     3301 ccaggctgac ggactcgaat gaccgcgagg ttcgtcgctt cgaaccgacc gttgcccgca
     3361 tcaacgagct cgagccggag tacgcctccc tcaccgatac cgacctgcgt ggcaagaccg
     3421 cgcagttccg cgcccggctg cgcgattcgc tcggcgacct gctcatcccg atcgagcagc
     3481 gcgcgctgac gccggagcag gctgaggagt cggagctggt cgcctccgat ccgatgcgga
     3541 tcagcgagga gcgcaaggag cagcacaagc gcgagcgggc gcagatcgac gccgcgctcg
     3601 acgagatcct gcccgaggcc ttcgcagcgg ttcgcgaggc gatgcggcgt gccctcgcca
     3661 agcgccatta cgacgttcag ctggtcggcg gcatggtcct tcacagcggt gccatcgccg
     3721 agatgaagac gggcgagggg aagaccttcg tggcgccgct ggccgcctac ctcaacggcc
     3781 tgaccggccg tggcgtccat gtcatcaccg tcaacgacta cctcgccaag cgggacgcgc
     3841 agtggatcgg ccaggtcttc tggcgtctgg ggataaccgt cggctcgatc cagcacgagg
     3901 ccgcctacct cgtcgaccct gacttccccc agaccgatga gcatctgcag gccctgcggc
     3961 cggtcgcccg cgccgaggca tacgcggcgg acgtgaccta cggcaccaac aacgagttcg
     4021 gcttcgacta cctccgggac aacctggtcg tcgaccttgg ccagcgggtc cagcgcgggc
     4081 acttcttcgc catcgtggac gaggtggaca acatcctcat cgacgaggcg cggacgccgc
     4141 tgatcatctc cggggcagcc gaggaatcgg ccgacaagta cgtccagttc gcccgcctgg
     4201 tgcctcgcct caaggcggag gaggactaca tcctggacga gaagttccgc caggtggcga
     4261 tcagcgaggc cgggaccgac aagatggagc gctggctggg ggtcgagaac ctgttcgacg
     4321 acgacttcag catggcgcgc cacctcgagc aggccctcaa ggcggaggcg ctgtacaagc
     4381 gcgaccgcga ctacgtggtc aaggacgacg aggtggtgat cgttgacgag ttcaccggtc
     4441 gcctgatgcc cggccggcgc tggagcgagg ggctgcacca ggcggtcgaa gccaaggagg
     4501 gggtgaagat ccagaacgag tcacgcacgc tggccaccat caccttccag aactacttcc
     4561 gcatgtacga caagctcgcc ggcatgaccg gcaccgccga gactgaggcg gaggaattcc
     4621 acaagatcta cgacctggag gtcgtggccg tccccaccaa ccagccgatg atccgggatg
     4681 acttcgcgga cctcgtcttt gccagccaga agggcaagtg ggcctccgtc gttgacgaga
     4741 tcgtcgagga gcacgagcgt ggacgcccgg tcctggtggg cacgatcagc gtcgaggtga
     4801 gcgagatgct tggcgagatg ctgaagcgac gcggcatcag gcacagcgtg ctgaacgcca
     4861 agttccatga gaaggaagcc gagatcgtgg cccaggcggg acacagcgac agcgtcacga
     4921 tcgccaccaa catggccggc cgcggcaccg acatcctgct gggcgggaac ccggagatgc
     4981 tcgccgcgga gctcctgcac aagaagggca ccaacgtgct ggaggcctcg ccagagcagt
     5041 acgccgccgc actggccgaa gccgatcgcc tgtgcgcaga ggatcgggag cgggtcatcg
     5101 ccgccggtgg cctgcacatc gtcggcactg agcgacacga ggcgcgccgc atcgacaacc
     5161 agcttcgcgg gcgcgccggc cgccagggcg acccggggag cacccgcttc tacctctcgc
     5221 tcgaggacga cctgatgcga cgcttcgcca gcgaccgcgt ccagtcgatc atgcgcaccc
     5281 tcggcttcaa cgacgagacg gcgctcgagt cgcgcatggt gagcaagacg atcgagggag
     5341 cacagacgcg ggtggagggc ttcaacttcg acacccgcaa gcacgtcgtg gagtacgacg
     5401 acgtcatcaa ccgccagcgc gagacgatct atcacgaacg cgagcgcatc ctccgctctg
     5461 acacgctggc gccgacggtg ctggccatgc tcgaggagga ggtccgggcg ctcgtcatcg
     5521 agcacaccgc cggcgagttc gcggccgaat ggaatcgcga tggcctcaag gcccagctga
     5581 ccgccatggt gccgaccctc gaccccgcgg cgcttgccgg gatcgacgac ctcgttgagg
     5641 cggaccagat ggcactcctc ctggtcgagc tggtggagga cacctaccag cgcaagcgca
     5701 ccgaggtcgg cgacgaggga atgctcgtcc tggagcgggt cgtgctgctg cgcgtcatcg
     5761 actcgctgtg ggtggagcac ctgacggcgg tcgacgacat gcggcgcggg atcggcctgc
     5821 gcgcgtacag ccagcgcgat cccctcaatg agttcaaggt cgaggcctat cgaatgttcg
     5881 acgagctgaa gtcgacgatc cgccacgaca tcacgcacac cgtcttccgc gtgaccctca
     5941 cccgcgaggc gccgcaccgc cacgagcggc ccatgatcga gagccggcca gacgtgacgg
     6001 gcgcggcggt gacggccggc gccgcgaccg cggccgtcgg gggcggtaac gggtcgcgcc
     6061 tgggtcccca gacggccaag gcggggccga agctcggccg caacgacccc tgctggtgcg
     6121 gatccggaaa gaagtacaag cgctgccacg gggcgtgaac ctcgacttgg ggggttggcg
     6181 aaccgagaca gaccggctac cattggcgct caggatcgcg aggcctcatg acctcgcgat
     6241 tcctcg
//
LOCUS       DATCQR010000008        16617 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_15933, whole genome shotgun sequence.
ACCESSION   DATCQR010000008 DATCQR010000000
VERSION     DATCQR010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 16617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16617
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15933"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..939)
                     /gene="lgt"
                     /locus_tag="VIN32_00220"
     CDS             complement(61..939)
                     /gene="lgt"
                     /locus_tag="VIN32_00220"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEY8921036.1"
                     /translation="MIDPIALQLGPLSVHWYGIIMATAILAAAWLGTNEAGRRGENPE
                     VGWSMLLWAVAGGVIGARIYHVIHQWDFYSANISLIPQVWNGGLGIPGAVIGGAIAIW
                     AYTRLRHLPTGRWFDIFVMALPLGQAIGRLGNFVNQELYGPPTDLPWGIPISAAHRVG
                     EWANLNLYPEATTRFHPLFAYEAIASLLTLGAMFWISRRFARWLYDGDMLLIYIMFYG
                     VVRSYLETFRVENWLIAGIPTATWLGLGGFVLAGGFLYLRHARGWGTPGAWIPQAEAQ
                     HREAQPSEPSPEAQPG"
     gene            1051..2433
                     /locus_tag="VIN32_00225"
     CDS             1051..2433
                     /locus_tag="VIN32_00225"
                     /inference="COORDINATES: protein motif:HMM:NF024742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8921037.1"
                     /translation="MQEPAADPTAADDRDEFGRPKTVLSWVQLLAVTAYWLAITVLWG
                     AFTFSVIPRLVESKGVLGSSTHPLAGLAIGFITTAGVLIAIIVQPTMGAISDHTRARI
                     GRRKPYILIGTAMDLLFLAFAAWAFWNQNYWAFVVAVALLQFSSNFAQGPYQGYIPDL
                     VPGRQVGIASGLLGAANIAGNLLGPGLAILFVAVLPGVLGFPEVTLGLFVAIGVVEVT
                     TMLITVFIVPDRPAPPTTKSLRQRALGAWGTDLLINRDYVWLLVSRLFVLTALVSLQA
                     FAVFFLENAHHMEPDAAQTSQFPIIITVAVAALLSAVPGGWISSRIGRKPVLFVAIGL
                     GIIGALVIAFGPDYWMVVAAAVPIGICSGVFLGVDWALMTDIIPKAESGRYMGISNIA
                     VASAGPIASTVGGLLVFFVSLATLGPELAYRAIFLLMGVELAIGAWALRRVTEPARGG
                     TLEARPVPAA"
     gene            2433..3182
                     /locus_tag="VIN32_00230"
     CDS             2433..3182
                     /locus_tag="VIN32_00230"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEY8921038.1"
                     /translation="MDPAERARRTGPRQLGVEVTTRYRVMRCLDVLVVRLLFDIRAEG
                     LERWPAAPFCLVLNHHNGWDPMLALAATPETPRVTWFGPKEADFSHGFRNRVMAFVGG
                     VIPYNPEKTTLTSAVRAVRRVFAAGGVLGIFAEGRVGFRETELLPFEEGAVAFAAASG
                     VPIVPCAIVGSTFMWFRRRVVFRYGEPIATAEARGREARVELDRAVRAAMQALMPRTE
                     PRLPRQRPLRFIGDLLTGPADLERRRAELGE"
     gene            3203..3475
                     /locus_tag="VIN32_00235"
     CDS             3203..3475
                     /locus_tag="VIN32_00235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921039.1"
                     /translation="MLPVIWAVFTVCMVGGFITIAAYWLDVQDRPDLTFRQRVAWSLG
                     ILLFPVVIPAYALFGGPGWPRPLLVSAFLPALALIMAGGLATGVLS"
     gene            3569..4978
                     /locus_tag="VIN32_00240"
     CDS             3569..4978
                     /locus_tag="VIN32_00240"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF006579.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEY8921040.1"
                     /translation="MTNLDTALAYLREHADEHLAGLDEFLRIPSVSADPQRRSDVRRA
                     AHWIADELTRIGAEHATITDTSSHPVVTADWLHAGPDAPTAIVYCHYDVQPTDPLGEW
                     VRPPFEPGYEDDIVYARGSGDDKGQLFMHLKAAEAWLKGVGSLPVNLRFFFEGDEEHE
                     AEPSETFVKTHPELLAGDLCIVSDSDMQDDEGTPAITYGLRGLAYWEVKVFGPFQDLH
                     SGQYGGGVDNPANVLARMLASLTDEHGGVTVPGFYDRVRELTPDEHAAYAAVPFDEAD
                     WAERIKVTGPMDGEPGSTLLERLSARPTFDINGLWGGYSGEGSKTIIPAFAGAKISSR
                     LVPDQDHREIEQLMVDHLKAIAPPTVRVEVKVIQGGQPVSTPLDHPALVIAAHALEET
                     FGRPPVFHRTGGSIPVVAAMERELGLPSIMIGFASPNGNFHAPNEWMPLANYRGGMDA
                     LVRLWQELGAVSPADLRPR"
     gene            4975..5616
                     /locus_tag="VIN32_00245"
     CDS             4975..5616
                     /locus_tag="VIN32_00245"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY8921041.1"
                     /translation="MTVASTDDPWLALGAASRLVGVAPDTLRRWADSGRVESFVTPGG
                     HRRFLRSALEAMMNAPRRHRYGVDRLTGSAGTISGEVHRRMARSGAAPPWQARLSAEQ
                     RADFRRWGQRTFNLVLEYVAAGKRAERTLLLGEAEKMGALYGAEASRAGLSLAEAVEA
                     FLFYRSPVLDAIASHLRRRAAELTDLTTAYREATAAIDEVLTALVASYREGVK"
     gene            complement(5686..6822)
                     /locus_tag="VIN32_00250"
     CDS             complement(5686..6822)
                     /locus_tag="VIN32_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014146084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEY8921042.1"
                     /translation="MPVISTAGARFADTVYADRLLRATVEAATRSIAALLVTPSADYE
                     YLLGYRPPAMERLTCLILPVEGVPALVVPRIEEPLARGAIGALAEGIEIIAWEEAEDP
                     FQLVKARLRGALRVGLQDQMWSRFVLQLLALLDPAELVDASPAIGAVRRVKQPEEIDR
                     LRAAASAADEAMLAITAERLSGRSEEEVSRRINELLLTAGHDTAEFAIVGSGPNSASP
                     HHSPGERVIEAGDAVVLDIGGIRDAYCSDTTRTAFVGEPPPDFAAMYEVLRVAQAAAC
                     AAVAPGVPARDVDRAARQIIDEAGYGEAFLHRTGHGIGLETHEEPYIVESNDEPLVAG
                     NAFSIEPGIYIADQWGARIEDIVVCTDAGGERLNTNRTELIVVE"
     gene            complement(6834..7253)
                     /locus_tag="VIN32_00255"
     CDS             complement(6834..7253)
                     /locus_tag="VIN32_00255"
                     /inference="COORDINATES: protein motif:HMM:NF025819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HEY8921043.1"
                     /translation="MKGLAVPSPVADDLMRHARAELPNEACGLISGSLDTGRATRFHP
                     ARNVEESPLRYNVHPEDLVRITFAIEEAGEELVAIFHSHTRSAAVPSATDRRTAMYPD
                     PFYLLASLADADAPPARALRAWRIYRGQTFEVPLTIG"
     gene            complement(7250..8179)
                     /locus_tag="VIN32_00260"
     CDS             complement(7250..8179)
                     /locus_tag="VIN32_00260"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HEY8921044.1"
                     /translation="MGDLLSTIGNTPMVELPHLAPSPRVRLYAKLESANPTGSVKDRI
                     ALTMLEEARETGTLQPGQTILEPSSGNTGISLAMIGRLLGHPVRIVMPDNTTVEREQL
                     LRLHGAEIVPSPGAEGSNGAIRLAQELSAADSSLFMPYQYGNPANPRAHQEGTGPEIL
                     AAVPDVDLFVAGLGTGGTLTGVGRFLKRERPGVRVIAAEPLPGEQVQGLRNLDEGFVP
                     PVLDASVLDDRYLVSNRDAVIGVRRLMRQEAIFAGLSSGAALHVALLAAAELGEGTVV
                     VLLADSGWKYLSTGIYDRDLDDLQDELDRSLLW"
     gene            complement(8184..8459)
                     /locus_tag="VIN32_00265"
     CDS             complement(8184..8459)
                     /locus_tag="VIN32_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007574850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like small modifier protein 1"
                     /protein_id="HEY8921045.1"
                     /translation="MSTVRIPPVLRETVGGSRTLAANGATVDEVLADLFANYPALRDR
                     VTADGELSPFVNVYVNDRDVRYRDGLETQVGPDDTVILLPAMAGGAR"
     gene            complement(8456..9550)
                     /gene="moeB"
                     /locus_tag="VIN32_00270"
     CDS             complement(8456..9550)
                     /gene="moeB"
                     /locus_tag="VIN32_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HEY8921046.1"
                     /translation="MRPATQLREVSAAEALELAASGWRILDVREQVEWNDGHIPTATL
                     VPLADLPSRIAEVAPERDEPLLVHCAVGARSLRASAWLIQNGYTNVASLKASLLDWRR
                     LGGAWEAPEQLLTPAQQRRYSRQVLIPEIGQAGQRKLLDAKVLLIGAGGLGSPAALYL
                     AASGIGTIGLVDDDVVDESNLQRQVLHTSDRVGIPKTESARMTLGALNPETKVVEHRE
                     RLDADNVERLITDYDVIVDGTDNFDTRYLLNDAAVRLRKPVVHGSIYRWDGQVTSFVP
                     FEGPCYRCMYPTQPPPELAPACDVAGVLGVLPGIAGLLQANEVFKLVLGVGETLAGRL
                     LMFDAMSTEFSEVRIWRDPACPACGEGTIQ"
     gene            complement(9553..9966)
                     /locus_tag="VIN32_00275"
     CDS             complement(9553..9966)
                     /locus_tag="VIN32_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEY8921047.1"
                     /translation="MDNLYRDFILEHYRNPHNKGTLDPHDLHFADSNPTCGDEMSMSL
                     VLDSSRAAIADVAFDGRGCAISQASASILTDGLRGQTLDEVREMNPKDLLDELGVPIG
                     PARLKCALLSYKVLQGAVHGGEVAWPAEAEAEAVG"
     gene            complement(9972..11240)
                     /locus_tag="VIN32_00280"
     CDS             complement(9972..11240)
                     /locus_tag="VIN32_00280"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEY8921048.1"
                     /translation="MAVEPALSALTPGAFSGTVVRSDFAVFDRPTRSGKRLVFLDAAA
                     SSPKPRSVVETMADAYSHHYANVHRGIYELSEDATGLFEAARGKVAAFIGAPSKREIV
                     FVRNATEAINLVAYSWGRANVHSGDRVVTTQLEHHANIVPWQQLTTEVGATLDYVAIS
                     DDGRLELDDLREKLAKGPKLLAVAAVSNALGTINPLAEIVKMAHEAGALVLVDAAQAA
                     PHMPIDVAALDCDFLALSGHKMLGPSGIGALWGRRELLDAMPPFMTGGSMIIKVTMEG
                     AEWNEVPYKFEAGTPAIVEAIGLGAAIDYLTNLGMDAVREHERYLFERAWNALGEIPG
                     VRRFGPDDAATHAGVISFVLDGIHPHDVATVFDSEGVAVRAGHHCAQPVMLRYDVPAT
                     TRASFYVYNDLDDVDALTAAVRATQTLFAG"
     gene            complement(11250..12107)
                     /gene="sufC"
                     /locus_tag="VIN32_00285"
     CDS             complement(11250..12107)
                     /gene="sufC"
                     /locus_tag="VIN32_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEY8921049.1"
                     /translation="MTDELEIKDLEVSIGQKKILKGLSLTVKVGEVHALMGPNGSGKT
                     SLAYTLMGHPDYHVDSGSITWAGEDLLNGPTPLGMPPDKRARLGLFLAFQYPSAIPGV
                     TVASFLRNIYNAVHHPKPVDESGEQSIKYTGIPLAKFRKLMEEKMALLHMDPAVASRY
                     VNDGFSGGEKKRLEMLQMAILNPRMAILDETDSGLDIDALRTVAEGINATLSPEMGVL
                     MITHYQRMLNYVKPQFVHVLLDGRVVMSGGEELSHKLEANGYDWVREQVGLPSGVGDE
                     VAEPVAEHV"
     gene            complement(12129..12629)
                     /locus_tag="VIN32_00290"
     CDS             complement(12129..12629)
                     /locus_tag="VIN32_00290"
                     /inference="COORDINATES: protein motif:HMM:NF014173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEY8921050.1"
                     /translation="MKLSTRAEYGIRVLVALAHADGDRPTSLAGIAKADKLPHAYIEQ
                     LVGALRRAGLVTATRGHSGGYRLARPAEQISLVDAVRALDGPILEMPCAGPDDLESCD
                     RPQDCSVHEVFERLHDSLSGMLGGTTLAEVAAGTGGPPYPPAVRRRVVAAHAKSQAAS
                     SAAPPN"
     gene            complement(12626..12943)
                     /locus_tag="VIN32_00295"
     CDS             complement(12626..12943)
                     /locus_tag="VIN32_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEY8921051.1"
                     /translation="MTTGFAAGLRASDVAPGTVVTVIVDGKSVCVGHTIDGRWGAIED
                     VCTHDGGDLGDGELDGDAVECPRHGGRFDLFTGRVLALPPVVPVRAYNVHVEGDEIKV
                     DLT"
     gene            complement(12940..14178)
                     /locus_tag="VIN32_00300"
     CDS             complement(12940..14178)
                     /locus_tag="VIN32_00300"
                     /inference="COORDINATES: protein motif:HMM:NF013614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HEY8921052.1"
                     /translation="MSVQAPAALDATTIEQVSRGEPAWLGTLRADWWRRVEANPWPTG
                     AEEEWRRTSLDGLPRDGGLLLDPPMATYDLDPALAARGVIFSDLATAVREHGDLVNGR
                     LGRFDTLPAQMPFWGRSLAAWTGGTFLYVPAGVAIDDVLTARITLPTGTYTYLPHTLV
                     VLEEDASATLLEEVASPDGESVWFGGTADLQIGYGARLRYANLQRLGDAVWRIGAQRV
                     EVGQDANVTTLNAEIGSAISKVGMDIRMTGKGGTSRLLGLLAAGEAQSIDFNSCQDLI
                     GSHTTSDLLYLSALYDASHASFYGVTRVRPEAKQTSSYQECRNLLLSPKAGAEPIPVL
                     EIETNDILRCGHGATAGAIDPVQRFYAQSRGMSADAAERMIVRGFFERVVAQIDSEPI
                     KARILDALVPRIGQTDEMAA"
     gene            complement(14184..15596)
                     /gene="sufB"
                     /locus_tag="VIN32_00305"
     CDS             complement(14184..15596)
                     /gene="sufB"
                     /locus_tag="VIN32_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013629660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEY8921053.1"
                     /translation="MALPNPAVDKVPTGYKYGWHDPEMKPLHVMKKGLSREVVEEISR
                     IKGEPQWMTDLRLKAYRHFEARPMPTWGGDMSQIDFQDIFYYVTATDKAVQSWDDVPD
                     YIRRTYDRLGIPEAEKKYLAGVGGQYDSEVVYHNIRADLEKLGVIFLGTDEALIKYPE
                     MFKEYFGTVIPANDNKLAALNTAVWSGGSFIYVPKGVHVELPLQAYFRINTENMGQFE
                     RTLIIADEGSSVHYVEGCTAPAYTTASLHSAVVEIIVKKGARVRYTTIQNWSHNVYNL
                     VTKRAVAYEDSVMEWVDGNLGSHLTMKYPSIYLLGERAHGEVLSIAFAGSDQHQDAGG
                     KVIHAAPNTTSLITSKSISQGTGRTSYRGLIKVYPGCHGSKSTVRCDALILSDEARSD
                     TYPYMEIDEDDVTIGHEASVSKVGSEQLFYLMSRGMNEAEASAMIVNGFIEPIVKELP
                     MEYAVEMNRLIQLQMEGAVG"
     gene            complement(15668..15940)
                     /locus_tag="VIN32_00310"
     CDS             complement(15668..15940)
                     /locus_tag="VIN32_00310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921054.1"
                     /translation="MIHRIVDGDATRGFTLQGDHNTAPDPWHPKWADMVGLARWRLPN
                     AGRLIALVQQPVILAGLASVLVVTVFLARPPRPRSSNGRVRQRLAG"
     gene            complement(16075..>16617)
                     /locus_tag="VIN32_00315"
     CDS             complement(16075..>16617)
                     /locus_tag="VIN32_00315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921055.1"
                     /translation="EGYPGHHAEASVKDDLLASAGHEELTLTTTLSPQAVVGEGMAGI
                     GREVVMSDDELGLELQRLARSINGRLDIEEELMVQRARRLLGPALGNAAVALHRDGEP
                     VDEVRSYLAEVGLVSDERLDETISQLADPVLRTRPFADIEGRRLVAEWLEVHGQTHGF
                     NRLLAEQQTPHALRSELNPA"
BASE COUNT         2491 a         5717 c         5704 g         2705 t
ORIGIN      
        1 agcgcaacca gcggcgtacc gccgacggct gcagtcacat cgggcaggat gccccgccgg
       61 tcagccaggc tgcgcctccg gggatggctc gctcggctgc gcctcgcggt gctgagcctc
      121 ggcctgaggg atccaggcgc ccggcgtccc ccagccgcgg gcgtgccgaa ggtagaggaa
      181 accccctgcc agcacgaacc cgcccagccc cagccaggtc gcggtcggga tgccggcgat
      241 cagccagttc tccacgcgga acgtctccag gtaggagcga accacgccgt agaacatgat
      301 gtagatgagc agcatgtcgc cgtcgtacag ccatcttgcg aagcgacggc tgatccagaa
      361 cattgccccc agcgtcagca ggctggcgat cgcctcgtac gcgaagaggg gatggaagcg
      421 ggtggtggcc tccgggtaca ggttgaggtt cgcccactcg cccacccggt gcgcggccga
      481 gatcgggatc ccccagggca ggtcggtggg cgggccgtac agctcctggt tgacgaagtt
      541 gccgaggcgc ccgatcgcct ggccgagggg caaggccatc acgaagatgt cgaaccagcg
      601 gccagtcggc aggtgccgca ggcgggtgta ggcccagatc gcgatcgcgc ccccgatcac
      661 cgctccgggg atcccgaggc cgccgttcca gacctgcgga atgagggaga tgttggccga
      721 gtagaagtcc cactggtgaa tgacgtgata gatgcgggcg ccgatgacgc caccggcgac
      781 cgcccacagg agcatcgacc agccgacctc ggggttctcg ccgcggcgcc ccgcctcgtt
      841 cgtccccagc caggcagcgg ccaggatggc ggtggccatg atgatcccgt accagtggac
      901 ggacaggggg cccagctgaa gggcgatcgg gtcgatcacg cggcggatga taccccgccc
      961 gcccgacgga gccactcgac cagcgagccg cagagtcttc cgcaaagagt ggcggcgtcg
     1021 taggctgcgc ctggaggaga ggagcgcacg atgcaggagc cagcggcaga tcccacggcc
     1081 gccgacgaca gggacgagtt cggccggccc aagaccgtcc tcagctgggt gcagctgctg
     1141 gcggtcaccg cctactggct ggccatcacg gtgctgtggg gcgccttcac cttcagcgtc
     1201 atcccgcgcc tggtcgaatc gaagggcgtc ctgggaagct cgacccatcc actggccggg
     1261 ctggcgatcg ggttcatcac caccgccggc gtgctgatcg cgatcatcgt gcagccaacc
     1321 atgggcgcca tcagcgacca cacgcgggcg cgcatcggcc gcaggaagcc atacatcctc
     1381 atcggaacgg cgatggacct tctcttcctg gccttcgccg catgggcgtt ctggaaccag
     1441 aactactggg ccttcgtggt ggctgtggcg ctactgcagt tcagcagcaa cttcgcccag
     1501 ggcccctacc agggctacat ccccgatctc gttccgggac gccaggttgg gatcgcctcc
     1561 ggcctgctcg gagcggcgaa catcgccggc aacctgctgg ggccgggcct ggcgatcctg
     1621 ttcgtggccg tgctccccgg ggtcctcggc tttcccgagg tcactttggg cctgttcgtg
     1681 gcgatcgggg tggtggaggt gaccaccatg ctgatcaccg tcttcatcgt ccccgaccgg
     1741 ccagcccctc ccaccaccaa gtcactccga cagcgcgccc tgggcgcgtg ggggactgac
     1801 ctgctcatca accgtgacta cgtgtggctc ctcgtcagcc gcctcttcgt gctgaccgcc
     1861 ctcgtctcgc tccaggcgtt cgcggtcttc ttcctggaga acgcgcatca catggagccg
     1921 gatgcggctc agacatccca gttcccgatc atcatcacgg tcgcggtggc ggcgctcctg
     1981 agcgccgtgc cggggggttg gatctcgtcg cgcatcggtc gcaagccggt cctgttcgtg
     2041 gcgatcggac tggggatcat cggtgcgctg gtcatcgcct tcggccctga ctactggatg
     2101 gtcgtggcgg ccgcagtccc gatcggcatc tgcagcggcg tcttccttgg cgtcgactgg
     2161 gcgctgatga ccgacatcat ccccaaggcc gaatcaggtc gctacatggg gatcagtaac
     2221 atcgcggtcg ccagtgccgg accgatcgcc agcacggtcg gcggcctgct cgtcttcttc
     2281 gtcagcctgg ccaccctggg gccagagctc gcctatcggg ccatcttcct gctgatgggc
     2341 gttgagcttg ccatcggcgc atgggctctg cgccgggtca ccgaaccggc gcgcggtggc
     2401 accctcgaag cgagaccggt gcccgccgcc tgatggaccc tgcggagcgg gcgcgacgca
     2461 cgggtccgcg gcagctgggc gtcgaagtca cgacacgcta tcgggtcatg cgctgccttg
     2521 acgtgctcgt cgtccgtctc ctcttcgaca tccgcgccga gggactcgaa cgctggccgg
     2581 ccgccccctt ctgcctggtg ctcaaccacc acaacggatg ggacccgatg cttgcgctgg
     2641 cggcgacccc ggagaccccg cgtgtcacct ggttcggacc gaaggaggcc gatttctcgc
     2701 acggcttccg caaccgtgtg atggcgttcg tcggaggcgt gatcccgtac aacccggaga
     2761 agacgaccct cacttcggcg gtgcgggcag tgcggcgcgt gttcgcggct ggcggcgtgc
     2821 tcggcatctt cgccgagggg agggtcggct tccgcgagac cgagctgctg ccgttcgaag
     2881 agggtgcggt ggcgtttgcc gcggccagcg gcgtcccgat cgtgccgtgc gcgatcgtgg
     2941 gcagcacgtt catgtggttc cggaggcggg tcgtctttcg ctatggcgag ccgatcgcga
     3001 cagccgaggc gcgcggtcgg gaggcacgtg tcgagctgga ccgtgcggtg cgggccgcga
     3061 tgcaggcgct catgcccagg acggagccgc gcctgccgcg gcagagaccg ctccgcttca
     3121 tcggcgacct gctgaccgga ccggcggacc tcgaacggcg gagggccgag ctcggggagt
     3181 aggcccgcgc tagcatcgcc gggtgcttcc cgtcatctgg gccgtgttca ccgtctgcat
     3241 ggtgggcggc ttcatcacca tcgcggcgta ctggctcgac gtgcaggatc gaccggacct
     3301 gacctttcgg cagcgcgttg cgtggtcgct cgggatcctc ctcttcccgg tcgtgatccc
     3361 ggcctatgcc ctcttcggcg ggcctggctg gccgcgtcct ctcctcgtca gcgcgttcct
     3421 gccggcactg gcactgatca tggcgggggg gctcgcgacg ggggtcctca gctaggaggc
     3481 ggctctcacc ctgcgagtga aggcccagcc cgagatcacc gctggacgcg cccgggactc
     3541 atcggttcca cggccctatc atccgcgaat gaccaatctc gacaccgcgc tcgcgtacct
     3601 gcgcgagcac gccgacgagc acctggcggg gctcgacgag ttcctgcgga tcccctccgt
     3661 cagcgccgat ccgcagcggc gcagcgacgt ccgccgcgcg gcgcactgga tcgccgacga
     3721 gctgaccagg atcggcgccg agcatgcgac catcaccgac accagcagcc atccggtcgt
     3781 caccgccgat tggctgcacg ctggaccgga tgcgcccacc gcgatcgttt attgccatta
     3841 cgacgtgcag ccgaccgacc cgctcggcga gtgggtccgg ccgccgttcg agccgggcta
     3901 cgaggacgat atcgtctacg ccaggggctc cggcgatgac aagggccaac tcttcatgca
     3961 cctaaaggcc gccgaggcgt ggctgaaggg cgtggggagc ctgcccgtca accttcgctt
     4021 cttcttcgag ggcgacgagg agcacgaagc ggagccctcc gagaccttcg tcaagacgca
     4081 tcccgagctg ctggccgggg acctctgcat cgtctcggat tccgacatgc aggatgacga
     4141 ggggacgccc gccatcacct acggcctgcg aggcctcgcc tactgggagg tgaaggtctt
     4201 cgggccgttc caggacctcc actcgggcca gtacggcggt ggcgtcgaca acccggccaa
     4261 cgtcctggcg cggatgctgg cgtctctgac cgatgagcac ggcggcgtca ccgtcccggg
     4321 cttctacgac cgggtgcgcg agctcacgcc cgacgagcac gcggcctacg cggcagtgcc
     4381 gttcgacgag gcagattggg ccgagcggat caaggtcacc ggaccgatgg acggcgagcc
     4441 gggctccacg ctgctcgagc gcctctcggc ccgcccgacc ttcgacatca atggactgtg
     4501 gggcgggtac tcaggcgagg gatcgaagac gatcatcccg gcattcgccg gggccaagat
     4561 cagcagccgg ctggttccag accaggacca tcgcgagatc gagcagctga tggtcgacca
     4621 cctgaaggcc atcgccccgc cgacggtccg ggtcgaggtc aaggtcatcc agggcgggca
     4681 accggtcagc accccgctcg accatccggc gctcgtgatc gcggcccacg cgctggagga
     4741 gaccttcggc aggccgccgg tcttccatcg cacgggtggg tcgatcccgg tggtggcggc
     4801 catggagcgg gagctcggac tgccctccat catgatcggc ttcgcatccc ccaacgggaa
     4861 cttccacgca ccaaacgagt ggatgccgct cgccaactac cggggaggca tggacgcgct
     4921 ggtgcgcctc tggcaggagc tgggagccgt ctccccggcg gacctgcgcc cgcggtgacc
     4981 gtggccagca ctgacgatcc gtggctggcg ctcggcgccg ccagccgcct ggtcggtgtg
     5041 gctcccgaca cgctgcgacg ctgggcggac tccggccggg tggagagctt cgtgacgccg
     5101 ggtggccatc gccgcttcct gcgatcggcc ctcgaagcga tgatgaatgc gccgcgccgg
     5161 caccgatacg gcgtcgatcg gctcaccggc tcggccggca ccatctcggg cgaggtgcac
     5221 cgccggatgg cgcgcagcgg tgcggcgccg ccgtggcagg ctcgcctcag cgccgagcag
     5281 cgggccgact tccgacgctg gggacagcgc accttcaacc tcgtgctcga gtacgtggcg
     5341 gctggcaagc gggcggaacg gaccctgctg ctgggtgagg cggagaagat gggcgccctc
     5401 tacggggcgg aggcatctcg cgccggcctc tccctggccg aggcggtcga ggccttcctc
     5461 ttctatcggt cgccagtgct cgatgccatc gcgtcgcacc tgcgccgtcg ggcggcggag
     5521 ctcacggacc tgaccacggc ctatcgagag gcgactgcgg ccatcgacga ggtgctgacc
     5581 gcgctggtcg cctcctaccg cgagggggtc aagtaggact gaccaagaac gcttcgggcg
     5641 cgattgcatg ctgtcagcga atacgccggg acgagccgcg tgcagctatt cgacgacgat
     5701 cagctcggtc ctgttggtgt tgagccgctc gccaccggca tcggtgcaga caacaatgtc
     5761 ctcgatccgg gcgccccact ggtcggcgat atagatcccc ggctcgatgc tgaaggcgtt
     5821 gccggccacc agcggctcgt cgttgctctc gacgatgtac ggctcttcgt gggtctccag
     5881 gccgatgccg tggccggtcc gatgcaggaa ggcttcgccg tagcccgcct cgtcgatgat
     5941 ttggcgcgcc gcccggtcga cgtcccgcgc ggggacgccc ggggcgaccg ctgcgcaagc
     6001 cgccgcctgg gccacccgca gcacctcgta catcgccgcg aagtccggcg gcggctcacc
     6061 gacgaacgcg gtgcgcgtgg tatcggagca gtaggcgtcc cggatgccac cgatatccag
     6121 gacgaccgcg tcgccggcct cgatgacccg ctctcccggg ctatggtgcg ggctggccga
     6181 gttgggtcca gagccgacga tcgcgaactc ggcggtgtcg tgtccggcgg tcagcagcag
     6241 ctcgttgatg cgccggctga cctcctcctc gcttcgcccg gagaggcgct cggcggtgat
     6301 ggccagcatc gcctcgtcgg ccgcggaggc cgccgcccgg aggcgatcga tctcctccgg
     6361 ctgcttgaca cgccggacgg cgccgattgc cggactggca tccaccagct ccgccggatc
     6421 gagcagggca agcagctgca acacgaagcg ggaccacatc tggtcctgca ggcccacgcg
     6481 cagagcgccg cgcaggcggg cctttacgag ttggaaggga tcctcggcct cctcccacgc
     6541 gatgatctcg atgccttccg ccagggcgcc gatggcaccc cgtgcaagcg gctcctcgat
     6601 ccgcgggacg acgagtgccg gcacgccctc caccgggagg atcaggcagg tcagccgctc
     6661 catcgccggg gggcgatagc cgagcaggta ctcgtagtcg gcggacggcg tgaccaggag
     6721 cgccgcgatg ctgcgggttg cggcttcgac ggtggcacgc agcagccgat cggcatagac
     6781 cgtatcggcg aagcgagccc cggcggtgga gatgacgggc atcgcgcaga aggttacccg
     6841 atcgtcagcg gcacctcgaa cgtctgcccg cggtagatcc gccacgcacg gagggcgcgc
     6901 gctggcggtg catcggcatc ggcgaggctg gcaagcaggt agaacgggtc ggggtacatg
     6961 gccgtgcgac ggtcggtcgc cgatggcacg gccgcagacc gcgtgtgcga gtggaagatg
     7021 gcgacgagct cctctcccgc ttcctcgatg gcgaaggtga tgcgcaccag gtcctcgggg
     7081 tgcacgttgt agcgcagtgg cgattcctcc acgttgcggg ccgggtggaa gcgggtcgcg
     7141 cgcccggtgt cgagcgatcc agagatgagc ccacaggcct cgttcggcag ctcggcgcga
     7201 gcgtggcgca tcaggtcgtc ggcgaccggt gatggcacgg caaggccctt caccacagca
     7261 gcgagcggtc cagctcgtcc tgcaggtcat cgaggtcacg gtcatagatg ccggtggaga
     7321 gatacttcca gccgctgtcg gccaggagca ccacgacggt cccctccccc aactctgccg
     7381 ccgcgagcag ggcaacatgc agggcagcac ccgacgagag gccggcgaag atcgcctcct
     7441 ggcgcatcag tcgccgcacg ccgatcaccg cgtccctgtt gctgaccagg tagcggtcgt
     7501 ccaggaccga tgcgtcgagc accggaggca cgaatccctc gtcgaggttc cgaagcccct
     7561 gcacctgctc tccggggagc ggctccgcgg cgatcacgcg caccccgggc cgctctcgct
     7621 tcaggaagcg gccgacgccg gtcagcgtcc cgccggtccc gagtcctgcc acgaacaggt
     7681 cgacgtcagg aaccgccgcg aggatctccg ggccggttcc ctcctggtgg gcgcggggat
     7741 tggcgggatt tccgtactgg tatggcatga agagcgacga atccgctgcg ctcagctcct
     7801 gggccaggcg aatggctcca ttcgagccct cggccccggg cgacggcacg atctcggcac
     7861 cgtgcaggcg cagcagctgc tcccgctcga cggtggtgtt gtccggcatc acgatccgca
     7921 ccggatgccc cagcaggcga ccgatcatgg ccaggctgat cccggtgttg ccggaggacg
     7981 gctccaggat ggtctgcccg ggctggagcg tcccggtttc gcgcgcttcc tcgagcatcg
     8041 tgagcgcaat gcggtccttg accgatcccg tgggattggc gctctcgagc ttcgcgtaga
     8101 ggcggacgcg gggtgacggg gcaaggtgcg gcagctccac catcggcgtg ttgccgatcg
     8161 tggagagcag gtcgcccatc gggctagcgg gcgcctccgg ccatggccgg cagcaggatg
     8221 accgtgtcgt ccggccccac ctgcgtctcc agcccgtcgc ggtagcggac gtcccggtcg
     8281 ttgacgtaga cgttcacgaa gggcgacagc tcgccgtccg ccgtcacgcg gtcgcggagg
     8341 gccggatagt tggcgaagag atcggcgagg acctcgtcga ccgtggcgcc gttcgctgcc
     8401 agcgtgcggc tgccgccgac cgtctcgcgc aggaccggcg ggatgcggac cgtgctcact
     8461 ggatcgtgcc ctcgccgcag gccgggcagg ccggatcacg ccagatccgg acctcactga
     8521 actcggtcga catcgcgtcg aacatcagca gccggcccgc cagggtctcc cccacgccga
     8581 gcaccagctt gaagacctcg ttggcctgga gcaggcccgc gatgcccggc agcaccccga
     8641 gcacgccggc cacgtcgcag gccggcgcca gctcgggcgg cggctgggtc gggtacatgc
     8701 agcggtagca cggcccctcg aacgggacga agctcgtgac ctggccgtcc cagcgataga
     8761 tcgagccgtg gaccaccggc ttgcggagcc tgaccgccgc gtcgttgagc aggtagcgcg
     8821 tgtcgaagtt gtcggtcccg tcgacgatca cgtcgtagtc agtgatcaac cgctccacgt
     8881 tgtcggcgtc aagccgctcg cggtgctcga ccaccttcgt ctcggggttg agcgcgccga
     8941 gggtcatgcg tgcgctctcg gtcttgggaa tccccacgcg gtcggaggtg tgcagcacct
     9001 ggcgctggag gttgctctcg tcgaccacgt cgtcatcgac caggccgatg gtgccgatcc
     9061 ccgatgcggc caggtagaga gccgccggcg accccagccc acccgctccg atcagcagca
     9121 ccttcgcgtc gagcagcttg cgctgtcccg cctgcccgat ctcggggatc agcacctggc
     9181 ggctgtagcg gcgctgctgc gcgggggtga gcagctgctc aggcgcctcc catgcccctc
     9241 cgagccggcg ccagtcgagg agcgacgcct tcaggctggc gacattcgtg tacccgttct
     9301 ggatcagcca ggccgatgct cggagcgagc gcgcaccgac cgcacagtgg accagcagcg
     9361 gctcgtcacg ctccggtgcg acctcggcga tgcgcgacgg caggtcggcg agcggcacga
     9421 gcgtggcagt cgggatgtgg ccatcgttcc actcgacctg ctcgcgtacg tcgaggatcc
     9481 gccatccgga cgcggccagc tcgagcgcct cggcggcgct cacctcacgg agttgagtag
     9541 cgggtcgcaa ggtcagccga ccgcctcagc ctcggcttcc gcgggccagg ctacctcgcc
     9601 gccgtggacc gcgccctgca gcaccttgta ggagagcagc gcgcacttga gccgtgccgg
     9661 accgatcggc acgccgagct cgtcgagcag gtccttgggg ttcatctcgc gcacctcgtc
     9721 cagggtctgg ccgcgcagcc catcggtcag gatcgaggcc gatgcctggc ttatggcgca
     9781 gccacgcccg tcgaacgcaa cgtcggcgat cgcggcgcgg gacgagtcaa ggaccaggct
     9841 catgctcatc tcgtcgccgc aggtcgggtt cgaatcggcg aagtggaggt cgtgcgggtc
     9901 cagggttccc ttgttatggg gattgcgata gtgctcgagg atgaaatccc ggtacaggtt
     9961 gtccattcag gtcaacccgc gaagagggtc tgggtcgcgc gcaccgccgc cgtcagcgcg
    10021 tcgacgtcgt caaggtcgtt gtagacgtag aagctcgctc gcgtggtggc cgggacgtcg
    10081 tagcgcagca tcaccggctg ggcgcagtga tggccggccc gaaccgccac gccctcgctg
    10141 tcgaagaccg tcgccacgtc atgcggatgg atcccgtcga gcacgaagct gatcacgcct
    10201 gcatgggtgg cggcgtcatc gggaccgaag cgtcggacgc cggggatctc gcccagggcg
    10261 ttccaggcac gctcgaacag gtagcgctcg tgctcgcgca ccgcatccat gcccaggttg
    10321 gtcaggtagt cgatggccgc gcccaggccg atcgcctcga cgatcgccgg cgtccccgcc
    10381 tcgaacttgt aggggacctc gttccactcg gccccctcca tggtgacctt gatgatcatg
    10441 ctgccgccgg tcatgaaggg cggcatcgcg tcgagcagct cgcgccggcc ccacagcgcc
    10501 ccgatccccg atgggccgag catcttgtgg cctgaaagcg ccaggaagtc gcaatccagg
    10561 gcggccacat cgatcggcat gtgcggcgcc gcctgggccg catccaccag caccagtgcg
    10621 cccgcctcgt gcgccatctt cacgatctcg gcgagcgggt tgatggtgcc gagcgcgttg
    10681 ctgaccgccg ccacggcgag cagcttcggt cccttcgcca gcttctcgcg caggtcgtcc
    10741 agctcgaggc gaccgtcgtc gctgatcgcc acgtagtcga gcgtggcgcc cacctccgtg
    10801 gtcagctgct gccacggcac gatgttggcg tggtgctcga gctgggtcgt caccacccgg
    10861 tcgcccgagt gcacgttggc gcgaccccag ctgtaggcga ccaggttgat cgcctcggtt
    10921 gcgttccgga cgaagacgat ctcgcgcttg ctgggtgcac cgatgaaggc cgccaccttg
    10981 ccgcgcgccg cctcgaacag ccccgttgcg tcctcggaca gctcgtagat gccgcgatga
    11041 acgttggcgt agtgatgcga ataggcgtcg gccatcgtct cgaccacgct gcgcggctta
    11101 ggggagctgg ccgccgcgtc caggaagacg agccgcttgc cgctccgcgt ggggcggtcg
    11161 aagaccgcga aatcggagcg cacgaccgtc ccggagaagg ctccgggggt cagcgcgctc
    11221 agcgccggtt cgacagccat cagcctatct tagacgtgct ccgccaccgg ctcggcgacc
    11281 tcgtcgccga cccccgatgg caggccgacc tgctcgcgca cccagtcgta tccattcgcc
    11341 tccagtttgt ggctgagctc ctcaccgccg gacatcacca ctcggccgtc gagcaggacg
    11401 tgaacgaact gcggcttcac gtagttcagc atgcgctggt agtgggtgat catgagcact
    11461 cccatctccg gcgagagggt ggcgttgatc ccctccgcca cggtccgcag cgcgtcgatg
    11521 tcgagcccgg agtccgtctc gtccaggatc gccatccgcg gattgaggat cgccatctgg
    11581 agcatctcga gccgcttctt ctccccgccc gagaagccgt cgttgacgta ccgcgaggcg
    11641 accgcgggat ccatgtggag gagcgccatc ttctcctcca tcagcttgcg gaacttggcg
    11701 agcgggatgc cggtgtactt gatcgactgc tcccccgact cgtcgaccgg cttgggatga
    11761 tgaaccgcgt tgtagatgtt tcgcaggaag ctggccaccg tcacgcccgg gatggcgctc
    11821 gggtactgga atgccaggaa gagccccagc cgcgcccgct tgtccggcgg catccccagc
    11881 ggcgtcgggc cgttcaggag atcttctccc gcccaggtga tgctccctga gtcgacgtgg
    11941 tagtcggggt ggcccatcag ggtgtacgcc aggctcgtct tcccggagcc gttcggcccc
    12001 atcagcgcgt ggacctcacc gaccttcacc gtcagcgaca gacccttgag gatcttcttc
    12061 tgaccgatgc tgacctcgag gtccttgatc tccagctcgt ccgtcatgct cgtctgcaac
    12121 agctcctgtc agttgggggg cgcggccgag gaggccgcct gggacttggc gtgggccgcg
    12181 acgacgcggc gccgcacagc aggcggatat ggaggcccgc ccgtgccggc cgccacctcg
    12241 gccagggtcg tcccgcccag catcccgctg agcgagtcgt ggaggcgctc gaagacctcg
    12301 tggacactgc agtcctgcgg ccggtcgcac gactcgaggt catccggtcc ggcgcacggc
    12361 atctcgagga tcgggccgtc gagggcgcgg actgcgtcga ccaggctgat ctgctcggcc
    12421 gggcgggcga ggcggtagcc accggagtgg ccgcgggtcg cggtcaccag gccggcgcgg
    12481 cgcagcgcgc cgacgagctg ctcgatgtag gcgtgcggca gcttgtccgc cttcgcgatg
    12541 ccggccagcg aggtggggcg gtcgccgtcg gcgtgcgcca gcgcgaccaa cacccggatc
    12601 ccgtactcgg cgcgggttga gagcttcatg tcaggtccac cttgatctca tcgccctcga
    12661 cgtgaacgtt gtacgccctg accgggacga ccggcggcag ggccagcacg cgcccggtga
    12721 agaggtcgaa gcggcccccg tggcgcgggc actccacggc gtccccatcc agctcgccgt
    12781 cgccgaggtc gccgccatcg tgcgtgcaga cgtcctcgat cgccccccag cgcccgtcga
    12841 tcgtgtgccc gacgcaaacg gacttgccgt ctacgatgac ggtcacgacg gtgccggggg
    12901 caacgtcgct ggcgcgcagg cctgccgcga accctgtcgt catgccgcca tctcgtcggt
    12961 ctggccgatg cgcggcacga gggcgtccag gatccgggcc ttgatcggct cggagtcgat
    13021 ctgggccacg acccgctcga agaagccgcg cacgatcatc cgctccgcgg cgtcggcgga
    13081 catgccgcgg ctctgcgcgt agaagcgctg gaccgggtcg atcgcgccag cggtggcgcc
    13141 gtggccgcag cgcaggatgt cgttcgtctc gatctccagc accgggatcg gctccgcccc
    13201 cgccttcggc gagaggagca ggttgcggca ctcctggtag gagctcgtct gcttcgcctc
    13261 gggccgcacc cgcgtgacgc cgtagaagga ggcgtgcgac gcgtcgtaca gagccgacag
    13321 gtagagcagg tccgatgtgg tgtggctgcc gatcaggtcc tggcagctgt tgaaatcgat
    13381 gctctgcgcc tcgccggcgg cgagcaggcc gagcagccgg ctggtcccac ccttgccggt
    13441 catgcggatg tccatgccga ccttgctgat ggccgagccg atctctgcgt tgagggtcgt
    13501 cacgttagcg tcctgtccca cctcgacccg ctgcgcgcca atgcgccaca ccgcgtcgcc
    13561 gagccgctgc aggttcgcgt aacgcagccg cgcgccgtag ccgatctgca ggtccgccgt
    13621 gccgccaaac cagaccgact ccccgtcggg ggacgcgacc tcctccagca gcgtggccga
    13681 ggcgtcctcc tcgaggacga ccagcgtgtg cggcaggtag gtataggtgc cggtcgggag
    13741 cgtgatgcgg gccgtcagca cgtcgtcgat ggccacgccc gcgggaacgt agaggaaggt
    13801 gccgccggtc catgccgcca gggagcgacc ccagaacggc atctgcgccg ggagggtatc
    13861 gaacctgccg aggcggccat tgaccaggtc cccgtgctcg cggaccgccg tcgccaggtc
    13921 gctaaagatg acgccgcggg cggcgagagc ggggtcgagg tcgtaggtcg ccatgggtgg
    13981 atcgaggagc aggccgccgt cgcgcggcag gccgtcgagc gaggtccggc gccactcctc
    14041 ctcggcaccg gtcggccacg ggttggcctc gacgcggcgc caccagtcgg cacgcagcgt
    14101 cccgagccag gcgggctcgc cacgggagac ctgttcgatc gtggtggcgt cgagcgccgc
    14161 cggcgcctgc acggacattc gcgctagccg accgcgcctt ccatctggag ctggatcagg
    14221 cggttcatct cgaccgcgta ctccatcggc agctccttga cgatcggctc gatgaagccg
    14281 ttcacgatca ttgcgctcgc ctcggcctcg ttcatgccgc ggctcatcag gtagaagagc
    14341 tgctccgagc cgaccttcga cacgctcgcc tcatggccga tcgtgacgtc gtcctcgtcg
    14401 atctccatgt atgggtaggt gtcggatcgg gcttcgtcgg agaggatcag ggcatcgcag
    14461 cggacggtcg acttcgaccc gtggcagccg gggtagacct tgatcaggcc gcggtagctc
    14521 gtcctgccgg tcccctgcga gatcgacttg gaggtgatca ggctggtcgt gttgggagcc
    14581 gcatggatca ccttcccgcc ggcgtcctgg tgctggtcgc tgccggcaaa ggcgatgctc
    14641 agcacctcgc cgtgtgcgcg ctcgccgagc aggtagatgg aggggtactt catggtgagg
    14701 tgcgacccaa ggttgccgtc gacccactcc atcaccgaat cctcgtacgc caccgcgcgc
    14761 ttggtgacga ggttgtagac gttgtggctc cagttctgga tcgtggtgta gcgcacccgc
    14821 gcgcccttct tgacgatgat ctcgacgacg gcgctgtgca ggctggcggt ggtgtaagcc
    14881 ggggcggtgc agccctccac gtagtgaacg ctgctccctt cgtcggcgat gatcagggtc
    14941 cgctcgaact ggcccatgtt ctcggtgttg atccggaagt aggcctgcag cggcagctcg
    15001 acatgcacgc ccttcggcac gtagatgaag ctgccaccgc tccacactgc ggtgttcagg
    15061 gcagcgagct tgttgtcgtt cgccgggatg acggtcccga agtactcctt gaacatctcg
    15121 gggtacttga tcagggcctc atcggtcccg aggaagatca cgccgagctt ctcgaggtcg
    15181 gcccggatgt tgtggtagac gacctccgag tcgtactgcc cgccgacgcc ggcgaggtac
    15241 ttcttctccg cctcggggat ccccaaccgg tcgtaggtgc ggcggatgta gtccggcacg
    15301 tcgtcccacg actggaccgc cttatcggtg gcggtcacgt agtagaagat gtcctggaag
    15361 tcgatctggc tcatatcgcc gccccaggtc ggcatcggcc gggcctcgaa gtggcggtac
    15421 gccttcaggc ggaggtcagt catccattgc ggctcgccct tgatccggct gatctcctcg
    15481 accacttcgc gagagagccc cttcttcatg acgtgcagag gcttcatctc ggggtcgtgc
    15541 cagccgtact tgtagccggt ggggaccttg tcgacggcag gattggggag ggccatggat
    15601 ctcctagtct gaatcccgac tcagacagtc ggtgttagac cgatgatgat ccctttcccc
    15661 cgcatggtca accggcgagg cgttgcctca cacgcccgtt gctgctcctc ggccgcgggg
    15721 gacgggcaag gaagaccgtg accaccaata cactggccag ccctgccagg atcaccggct
    15781 gctgcacgag ggcgatcagg cggccggcgt tcggcagccg ccacctcgcc aggccgacca
    15841 tgtcagccca ttttggatgc caggggtcag gcgcggtgtt gtggtcgccc tggagcgtga
    15901 agcccctggt tgcgtcgcca tcaacgatcc gatggatcac gaccctgccg gctccgacct
    15961 ggcctacggg tacgcgttag gcgaccacct cgcttttgat cgctggaaca agttccgcga
    16021 catgcgccaa aaagaccata gcgggggagt actcgcggat gggtccgggt ggcactaggc
    16081 cggattgagc tcggagcgca gcgcgtgcgg ggtctgctgc tctgccagca gccggttgaa
    16141 gccatgggtc tggccatgca cctccagcca ctcggcgacg agacgccggc cctcgatgtc
    16201 cgcgaacggc cgggtgcgca ggacgggatc agccagctgg gaaatcgtct cgtccaggcg
    16261 ctcgtcgctg accaacccca cctccgcaag gtacgagcgc acctcgtcga cgggctcgcc
    16321 atcgcgatgc agggccacgg ccgcgttccc cagggcggga ccgagcaggc gtcgggcgcg
    16381 ctgcaccatc agctcctcct caatgtccag gcgtccgttg atcgagcggg ccagccgctg
    16441 cagctccaga ccgagctcgt cgtcgctcat aaccacctcg cggccgatcc cggccatccc
    16501 ctcgccgacc accgcctgcg gagagagcgt cgtcgtcagc gtgagctctt cgtgcccggc
    16561 tgacgccagc aggtcgtcct tgaccgatgc ctccgcgtgg tggcccggat agccctc
//
LOCUS       DATCQR010000009         2626 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_155331, whole genome shotgun sequence.
ACCESSION   DATCQR010000009 DATCQR010000000
VERSION     DATCQR010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2626
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155331"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..425
                     /locus_tag="VIN32_00320"
     CDS             <1..425
                     /locus_tag="VIN32_00320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921056.1"
                     /translation="LAPTVAPSFPPTTPKPSDGGGPVVFPDGFTARTEPCAQEPSSQD
                     GCNSSGATISGGSVWVWVGWRKGNNSDTIGATIVDAAGTAVADGHIPLSSIGENGCGP
                     SCNGWARFKFSGLAVGNYTIRVDRNGTLAAEATFTVTG"
     gene            complement(422..1498)
                     /gene="speB"
                     /locus_tag="VIN32_00325"
     CDS             complement(422..1498)
                     /gene="speB"
                     /locus_tag="VIN32_00325"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018504257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HEY8921057.1"
                     /translation="MADETRRHSGPNDEDPATGLDPYTEYDDFDLPAYVGRTSFQKLP
                     ELTDGDALRARHPDVAIVGAPFDDAVSNRPGARFGPRAIRAATYHAGSPNSLQLGIEP
                     FEWMDVVDAGDAPVVPANLERGHAVIRRKVLEVAQAGAIPIVLGGDHSITFPSASAVA
                     EAVAPRRLGMVHFDAHADAANSTWGVLASHGTPMRRLIESGAIEGRNFVQVGLRGYWP
                     PPATLEWMQRHGLRVHFMTEIEERGAEAVVADAIAEALDGPELIYLSIDIDVIDPGMA
                     PGTGTPEPGGMLSRELLRAIRQIVAAVDLAGMDVVEVAPVYDVSEITAAAAHRCVMEA
                     ISALAAKRRDAGGAARPSGRSRDG"
     gene            1629..2024
                     /locus_tag="VIN32_00330"
     CDS             1629..2024
                     /locus_tag="VIN32_00330"
                     /inference="COORDINATES: protein motif:HMM:NF021486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2203 domain-containing protein"
                     /protein_id="HEY8921058.1"
                     /translation="MPAARRTYTIDEANALVPQVRAVLLQLAVEQRRLDASHAEMHRQ
                     LDANGDPESASAAGRQETETAEIREGMRTLLVHLSEMGVEVRDLEMGLVDFPGERDGE
                     RVWLCWRLADARVAFWHPPDEGYATRRPW"
     gene            2035..>2626
                     /locus_tag="VIN32_00335"
     CDS             2035..>2626
                     /locus_tag="VIN32_00335"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HEY8921059.1"
                     /translation="MAITVIGDSTLDVTVRAAEAPRAGGDVPARISISPGGQGANVAV
                     RLARARADVRLATAVADDAAGRMLAEALEADGVAVVQLGAERSGLVISLLDADGERAM
                     LSDRVTLQPTRLAEACAGADWVHCSGYALADDATGDAVAGILGSLPTTTPVSAGGGSL
                     PDDPARSARVRARLATARVALLVLSRDEAAALLDRPL"
BASE COUNT          377 a          912 c          949 g          388 t
ORIGIN      
        1 gcctagcacc gaccgtggcg ccgtcattcc ccccgaccac gccgaaaccc tctgacggcg
       61 gaggcccggt tgtcttccca gatggcttca ccgcgcggac ggagccgtgc gcgcaggagc
      121 cgtcaagcca ggatggttgc aactcgagcg gagcgacgat cagtggtggg tcggtgtggg
      181 tatgggtcgg ctggcggaag ggcaataact ccgacacgat cggcgcgacc atcgtggacg
      241 ccgcaggcac cgccgtggcg gacggccaca tcccgttgag ctccatcggc gagaacggat
      301 gcgggccttc ctgcaacggc tgggccaggt tcaagttcag cggtctggcc gtggggaact
      361 acaccatccg cgtcgatcgc aacggcacac tggcggccga ggcgacgttc accgtcaccg
      421 gctagccgtc gcgggagcgc ccgctcggtc gggcagcgcc tccggcatcg cgccgcttgg
      481 cggcgagggc gctgatggcc tccatcacgc accgatgggc ggccgcggcc gtgatctcgc
      541 tcacgtcgta gaccggcgcc acctcgacca cgtccatgcc cgccaggtca acggcagcca
      601 cgatctggcg gatggcccgc agcagctcgc gtgacagcat ccctccgggc tccggcgtgc
      661 cggtcccggg cgccatcccc gggtcgatca cgtcgatgtc gatcgacagg tagatcagct
      721 cgggtccgtc gagcgcctcg gcaatggcat cggccaccac cgcctccgct ccgcgttcct
      781 cgatctcggt catgaagtgg acccgcaggc cgtgccgctg catccattcg agggtggccg
      841 gcggcggcca gtacccgcgc aggccgacct gcacgaagtt gcgcccctcg atcgcgccgg
      901 actcgatcag ccgtcgcatc ggcgttccgt gtgacgcgag aactccccag gtgctgttgg
      961 cggcgtcggc gtgcgcgtcg aagtgaacca tccccagccg ccttggggcg accgcctcgg
     1021 caacggcaga tgcggaggga aacgtgatgg aatgatcgcc gccgagcacg atcgggatcg
     1081 cgccggcctg cgccacctcc agcaccttgc gccggatgac cgcgtggcca cgctcgaggt
     1141 tggcggggac caccggggcg tccccggcgt cgaccacgtc catccactcg aacggctcga
     1201 tgccgagctg cagcgagttg ggcgaccctg cgtgataggt cgcggcgcgg atcgcgcgcg
     1261 gcccgaacct cgcgcccggc cggttgctga ccgcgtcatc gaacggggcg ccgacgatgg
     1321 cgacgtccgg gtgacgcgct cgcagcgcgt caccatcggt cagctcgggg agcttctgga
     1381 agctggtgcg gccgacgtac gcgggaaggt cgaagtcatc gtactcggtg tacggatcca
     1441 gcccggtcgc tgggtcctcg tcattggggc cgctgtggcg gcgcgtctca tcggccatcg
     1501 gtgatggctg acgccctcct gcgaaacgat cgcgggcgcc gtcgcggcgc ggagcaggcc
     1561 cgcagctgaa gcggtcccga tcctaccaga ggggtccagc gaaatggtcc tgctaccatc
     1621 catcggccat gccagccgcg cgccggacgt acacgatcga cgaggcgaat gccctggtcc
     1681 cgcaggtacg cgccgtgctc ctgcagctcg cggttgagca gcgacgcctc gatgcatcac
     1741 atgccgagat gcatcgccag ctcgacgcca atggggaccc cgagagtgcc tccgcggcgg
     1801 gtcgtcagga aacggagacg gcggagataa gggaggggat gcgcaccctg ctggtgcatc
     1861 tcagcgagat gggggtcgag gtgcgggacc tggagatggg cctcgtcgac tttccgggcg
     1921 aacgggacgg cgaacgcgta tggctgtgct ggaggctggc cgacgcccga gtcgccttct
     1981 ggcacccccc cgatgagggc tacgcgacgc ggcggccctg gtgaccctcg gctgatggcg
     2041 atcaccgtca ttggcgactc gaccctggac gtcacggtcc gtgcagcgga ggctccccga
     2101 gccggtggcg acgtcccggc caggatctcg atctcccccg gggggcaggg agccaacgtt
     2161 gcggtgcggc tggctcgcgc ccgggcggac gtccggctgg ccaccgccgt cgccgacgac
     2221 gcggccgggc gcatgctggc tgaagcgctc gaggccgatg gggttgccgt cgtgcagctt
     2281 ggggccgagc ggagcggcct ggtcatctcg ctgctcgatg ccgacggcga gcgggcgatg
     2341 ctctccgacc gcgtcacgct ccagccgacc cgcctggccg aggcatgtgc cggagcggat
     2401 tgggtccact gttccgggta cgcgctggcc gacgatgcca cgggcgacgc cgtggccggc
     2461 atcctcgggt cgctgccgac aacgacccct gtgagtgcag gcggcgggtc gttgcccgac
     2521 gacccggcac ggtccgcgcg ggtgagggcg cgcctggcga ccgctcgagt cgccctgctg
     2581 gtcctgtccc gggatgaggc ggcggcgctg ctcgatcggc ctctgc
//
LOCUS       DATCQR010000010         6092 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_171065, whole genome shotgun sequence.
ACCESSION   DATCQR010000010 DATCQR010000000
VERSION     DATCQR010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6092
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171065"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(110..1327)
                     /gene="hutI"
                     /locus_tag="VIN32_00340"
     CDS             complement(110..1327)
                     /gene="hutI"
                     /locus_tag="VIN32_00340"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017506205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HEY8921060.1"
                     /translation="MSGADLVVHNIGELVTCDPAQGEAPGVIRDAALAATDGVITYVG
                     PASDLLEGTGAREIDAGGAAVAPGFVDCHTHAVWLGDRADEYAQRAAGATYEEIAAAG
                     GGIVSTVRATAAGTVDELVAAARPRLELMAREGTTTVEIKSGYGLDRDAEMGQLEAAD
                     RLGREPGLPDVVATYLPLHAAPPGDRAAFLDRVIEQDLPHAAPRARFADCFCETGAFS
                     VEECERFLVAAKAAGLRLKIHTEQLSHSGGAALAARLAAASADHLEHASDDDMRALAA
                     AGVVGVILPGAALTLGGPPPPGRRLLDAGVTVAIATDCNPGTSNTESMPLMVSLAVAL
                     AGLTPAEALVAATSGGAAALQLGDRGVLRVGAHCDLAILESDRWLDIAYHLGSGGVAT
                     TVRRGEVISGAGG"
     gene            complement(1324..2226)
                     /locus_tag="VIN32_00345"
     CDS             complement(1324..2226)
                     /locus_tag="VIN32_00345"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HEY8921061.1"
                     /translation="MNAREWLSRGTPQGLPDLAVLGAPIHRASISLTSAHTTPPAIRA
                     ALERFSTWDGDHNVQLEDLRVVDLGDIEGDDADPDATAAHGRIQAAVADAFKRAPVVA
                     VFGGDNSLTRPAMAGAAHRGLGESWGLITLDAHHDCRPLDDGASNGSPVRGLITDGLP
                     GSRVAQIGIRGFANHEDHAAWALAQGIDVRTADEVRAEGMAATLHHVLADLRVENVTD
                     IYADIDIDVLDRAFAPACPASLPGGLRPPDVLEAAYILGADPAVRAVDFVEVDAAADV
                     AGITLRVTAAVFLAFCAGLTKRLR"
     gene            complement(2223..3899)
                     /gene="hutU"
                     /locus_tag="VIN32_00350"
     CDS             complement(2223..3899)
                     /gene="hutU"
                     /locus_tag="VIN32_00350"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013223168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HEY8921062.1"
                     /translation="MKGARPVRAPRGNQISCASWQIEGALRCLMNNLDPEVAEDPDNL
                     IVYGGRGKAARNWECFDAIVASLRSMRPDDTLLVQSGKPVGVARTHEMAPRVLIANSL
                     LVPDWATPDEFWRLEAEGLTMYGQMTAGSWIYIGTQGILQGTYETFAAIARKRFGGSL
                     AGRLVLTSGLGGMGGAQPLAVTMNDGVALCVEVDPARLERRLETGYLDERADSLEDAV
                     RRCDLAREERRALSVGVVANAAEALPALLASGLAVDVVTDQTSAHDPLFGYVPAGMSL
                     DDAAQLRAADPDGYLKRARVSMALHCQAMVDFQARGAEVFDYGNSLRAEAKLGGYEHA
                     FDYPGFVPAYIRPLFCEGIGPFRWVALSGDPADIAATDRAILELFPANESLARWMRLA
                     AERVQFQGLPARICWLGAGERHLAGIRFNEMVASGELSAPVVIGRDHLDSGSVASPLR
                     ETESMRDGSDAIADWPILNALLNASSGASWVAVHHGGGVGIGRSIHAGAQVVADGTPE
                     AARRLELVLTNDPASGVMRHADAGYDEAWAAAAARDVRIPMAPGAAGVAD"
     gene            complement(3896..5428)
                     /gene="hutH"
                     /locus_tag="VIN32_00355"
     CDS             complement(3896..5428)
                     /gene="hutH"
                     /locus_tag="VIN32_00355"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006932411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HEY8921063.1"
                     /translation="MRIDGSPLTIDQVVDVARRGERVEVAEAARQSMQPSLAVVERLH
                     DSDASIYGVTTGFGALADTAIAVEDRAILQRAIVLSHSAGMGAPVDDEVVRGMMLLRA
                     RSLTAGYSGVRPELVEAIVAVLNAGIRPYVPEYGSLGASGDLAPLAHAASCLLGEGWV
                     VGPDGTPAPAGPALVDGGIVPLKLRPKEGLALINGTDGMAATLALAVHDLSELLTAVD
                     ATGAMSIEALLGTPVVFEEAIVALRASPGQRDSAANIRRLLDGSAIVASHRESHHAVQ
                     DAYSLRCAPQVHGAARDVVGFARQTVERELSAVVDNPVVLGDQVVSSGNFHGQALAYA
                     ADMCASVCADVAAIAERRVDRLLDPARSGGLPAFLVDNPGVNSGLMIAQYTAAAIVAS
                     LRAAATPLAAQSMSASAGQEDHVSMGWTAALRTRRSVAELRRVIAVEAVCAAQALDLR
                     ESDAALRAGPGTAALRDSLRGRVEHLDQDRFLAPDLAAAEAWLASPEWRAGAEAAIGA
                     LL"
     gene            5501..6043
                     /locus_tag="VIN32_00360"
     CDS             5501..6043
                     /locus_tag="VIN32_00360"
                     /inference="COORDINATES: protein motif:HMM:NF021996.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DM13 domain-containing protein"
                     /protein_id="HEY8921064.1"
                     /translation="MKHGTSVFSRHRLAVIAAFVVVAVAAGIPVTAYVIVPLFVRSTV
                     VERAPSTTPSAAGTSTPDTASTPAVAGNTTLSTGTLQRISAVDFGTGKVSILQVSRRR
                     FLRFENVEISAAPAIHVYLSDRIDGSPGTFTDLGVLKATSGSFNYVIPTTVDLSRVKS
                     VVAWCQQFTVTITYAVLQPT"
BASE COUNT          861 a         2186 c         2211 g          834 t
ORIGIN      
        1 gtgacgtgcg ccccccggac gcccaccgac gcgctgacgc cgcgcttgct gaagttgagg
       61 cgcagccccg gcaagatctg cttgcgccgg tagaaccgga ggtatcccat cagccgccgg
      121 ctcccgagat gacttcgccg cgccgcaccg tggtggcaac gccgccagag ccgaggtggt
      181 aggcgatgtc gagccagcga tcgctttcca ggattgccag gtcgcagtgg gcgccgaccc
      241 ggagcacccc tcggtcgccg agctgcagcg ccgccgcgcc acccgaggtc gccgcgacga
      301 gggcctcggc cggggtcagg ccggcgagcg ccaccgccag cgagaccatc aggggcatcg
      361 actcggtgtt gctggtgccg gggttgcagt cggtggcgat ggcgacggtc acaccggcgt
      421 cgaggagccg gcggccgggt ggaggcggac cccccagggt cagcgcggcc ccgggcagga
      481 tcacgccgac gaccccggcg gctgcgagcg cccgcatgtc gtcgtccgac gcatgctcga
      541 gatggtcggc gctggcggct gcaagccgag ccgccagcgc cgcgccgccg ctgtggctga
      601 gctgctcggt gtggatcttg agccgcagcc cggcggcctt ggccgcgacc aggaaccgct
      661 cgcactcttc gaccgagaag gcgccggtct cgcagaagca gtcggcgaag cgcgcgcggg
      721 gtgcggcgtg agggaggtcc tgctcgatga cgcggtcgag gaaggcggcc cggtcgcctg
      781 gcggcgcggc gtgcagcggc aggtaggtag cgaccacgtc ggggaggccg ggctcccggc
      841 cgagccggtc ggcggcttcg agctggccca tttccgcatc gcggtccagc ccgtatccgc
      901 tcttgatctc gacggtcgtg gtgccctcgc gcgccatcag ttccagtcga ggccgcgccg
      961 ctgccaccag ctcgtcgacg gtgccggctg cggtggcgcg tacggtcgag acgatcccac
     1021 cgccggccgc ggcgatctct tcgtaggtcg cccccgcagc gcgctgcgca tactcgtcgg
     1081 cccggtcgcc gagccagacc gcgtgggtgt ggcaatcgac aaagcccggc gccaccgccg
     1141 cgccccccgc gtcgatctcc cgcgccccgg ttccctctag aaggtccgac gcgggtccca
     1201 cgtaggtgat caccccgtct gttgccgcca gcgcggcgtc ccggatgacg ccgggggcct
     1261 cgccttgggc cgggtcgcag gtgaccagct cgccgatgtt gtgaacgacg aggtccgcgc
     1321 cgctcaccgc aagcgtttgg tcagcccggc gcagaaggcc aggaacaccg ccgccgtcac
     1381 ccgcagggtg atcccggcca cgtcagccgc cgcatcgact tcgacgaagt cgaccgcccg
     1441 caccgcgggg tcggcgccca ggatgtaggc agcctccagc acgtcgggcg gtcgcagccc
     1501 gccgggcagg ctggccgggc acgcgggggc gaaggcgcgg tcgaggacgt cgatatcgat
     1561 atcggcatag atgtcggtga cgttctcgac tcggaggtcg gccagcacgt ggtgcagggt
     1621 cgccgccatg ccctcggcgc gcacctcgtc cgcggtgcgg acgtcgatcc cctgcgccag
     1681 cgcccaggcg gcgtggtcct cgtggttggc gaagccacgg atgccgatct gggcgacgcg
     1741 gctcccgggc aggccatcgg tgatcaggcc acggaccggg ctgccgttgc tggcgccgtc
     1801 gtcgaggggg cggcagtcgt ggtgggcgtc gagcgtgatc agcccccagc tttcgccgag
     1861 tccgcggtgg gcggcgcccg ccatcgccgg tcgggtcagg gagttgtcgc cgccaaaaac
     1921 ggcgaccacc ggggcgcgct tgaaggcgtc cgccacggcg gcttggatgc gcccgtgcgc
     1981 cgccgtcgcg tcagggtcgg cgtcgtcacc ctcgatgtcg ccgaggtcga ccacgcgcaa
     2041 gtcttcgagc tggacgttgt ggtccccgtc ccaggtcgag aagcgctcca gggcggcacg
     2101 aatcgccggc ggtgtcgtgt gcgcgctggt caggctgatc gaggcgcggt ggatgggcgc
     2161 gcccagaacg gcgaggtccg gcagcccctg cggggtgccg cgggagagcc actcgcgcgc
     2221 gttcaatccg ccaccccagc tgcacccgga gccatgggga tgcgcacatc ccgcgccgcg
     2281 gccgccgccc aggcctcgtc atagccggcg tcggcgtggc gcatcacgcc gctggcggga
     2341 tcgttggtca gcaccagctc caggcggcgc gccgcctcgg gggtcccgtc ggcgaccacc
     2401 tgcgccccgg cgtggatcga tcgcccgatg cccaccccgc cgccgtggtg caccgccacc
     2461 cacgacgccc cgctggaggc gttgagcagc gcgttcaaga tcggccagtc ggcgatcgcg
     2521 tcgctgccgt cgcgcatcga ctccgtctcc ctaagcggcg aggccacgct gccggaatcg
     2581 aggtggtcgc ggccgatcac caccggcgcc gagagctcgc cgctggcgac catctcgttg
     2641 aagcgaatcc cggcgaggtg ccgctcgccg gcgccgagcc agcagattcg cgccggcagg
     2701 ccctggaatt gcacccgctc ggcggccagc cgcatccagc gagccaggct ctcgttggcc
     2761 gggaacagct cgaggatggc gcgatcggtg gcggcgatgt cggcgggatc gccgctgagg
     2821 gcgacccagc ggaagggccc gatgccctcg cagaagagcg gtcggatgta ggcgggcacg
     2881 aagccggggt agtcgaaggc gtgctcgtag ccgccaagct tggcctcggc ccggagcgag
     2941 ttgccgtagt cgaagacctc ggcgccgcgc gcctggaaat ccaccatcgc ctggcagtgc
     3001 agcgccatcg acacgcgagc acgcttgagg tagccgtcgg ggtcggcggc gcgaagctgc
     3061 gccgcatcgt ccagcgacat ccccgcgggg acgtagccga acagggggtc gtgggcgctg
     3121 gtctggtcgg tgacgacgtc gaccgccagc ccgctcgcca gcagcgccgg aagcgcctcg
     3181 gcagcgttgg cgacgacgcc gaccgacagc gcgcggcgct cctcgcgagc caggtcgcag
     3241 cgccgcaccg cgtcctccag cgagtcggcg cgctcgtcga ggtagcccgt ctcgaggcgg
     3301 cgttccagcc gggccgggtc gacctcgacg cacagcgcca cgccgtcgtt catggtcacc
     3361 gccaggggct gcgccccgcc catcccgccg agccctgagg tcagcaccag ccgccccgcg
     3421 agcgagccgc cgaagcgttt ccgagcgatc gcggcgaagg tctcgtaggt cccctggagg
     3481 atgccctggg tgccgatgta gatccacgaa ccggcggtca tctggccgta catcgtcagc
     3541 ccctcggcct ccagccgcca gaactcgtcc ggcgtggccc agtccgggac cagcaggctg
     3601 ttggcgatca ggacccgcgg cgccatctcg tgcgtccggg ccacgccgac cggcttgccc
     3661 gactgcacca gcaaggtgtc gtcagggcgc atcgaccgca ggctcgcgac gatggcgtcg
     3721 aagcattccc aattgcgcgc ggccttgccc cggccgccgt agacgatgag gttgtcgggg
     3781 tcctcggcaa cctcggggtc gaggttgttc atcaggcagc gcagcgcgcc ttcgatctgc
     3841 cagctggcgc aagagatctg gttgccacgc ggcgcccgca ccgggcgcgc gcccttcaca
     3901 gcagcgcccc gatggcagcc tcggcgccgg cgcgccactc cggcgacgcc agccaggcct
     3961 cggcggcggc gaggtcgggc gccaggaatc ggtcctggtc gaggtgctcg acgcgacccc
     4021 ggagtgagtc ccggagcgct gctgtgcccg gcccggcccg cagcgcagca tcggattccc
     4081 gcaggtccag ggcctgggcg gcgcagaccg cctcgaccgc gatcacgcgc cggagctcgg
     4141 cgacggagcg gcgcgtccta agcgccgccg tccatcccat cgagacatga tcctcctggc
     4201 cggcgctggc cgacatcgac tgtgccgcca gcggggtggc ggcggcgcgc agcgaggcga
     4261 cgatggcggc ggcggtgtac tgcgcgatca tcaggccgct gttcacgccc gggttgtcga
     4321 ccaggaacgc cggcaggcct ccggagcgcg cagggtcgag caggcgatcg acgcgacgct
     4381 cggcaatcgc ggcgacgtcg gcacacaccg aggcgcacat gtcggcggcg taggcgagcg
     4441 cctggccgtg gaagttgccg ctgctgacca cctggtcgcc gaggacgacg gggttgtcga
     4501 ccacggcgct gagctcccgc tccaccgtct gccgcgcgaa cccgaccacg tcgcgcgcgg
     4561 cgccgtggac ttgcggggcg caccggagcg agtaggcatc ctgcaccgcg tggtgggact
     4621 cgcggtgcga ggcgacgatg gcggagccgt cgaggaggcg acggatgttg gccgccgagt
     4681 cgcgctgtcc aggcgaggca cggagggcga cgatggcctc ctcgaagacc acgggggtgc
     4741 cgagcagggc ttcgatgctc atcgcgccgg tggcgtcaac cgcggtgagc agctcgctga
     4801 ggtcgtggac ggccagggcg agggtggcgg ccatgccgtc ggtgccattg atcagcgcca
     4861 gcccctcttt gggccggagc ttgagcggca cgatcccgcc atcgacaagc gccgggcccg
     4921 cgggcgccgg cgtcccgtcg gggccgacca cccagccttc accgagcagg caggacgccg
     4981 cgtgggcgag gggggcgagg tcgccggatg cccccagcga cccgtactcg gggacgtagg
     5041 gacggatgcc ggcgttcagc accgcgacga tggcctcgac cagctcgggc cgcacgccgg
     5101 agtagccggc ggtcagcgac cgggctcgga gcagcatcat gcctcgcacc acctcatcgt
     5161 cgacaggcgc ccccatcccc gcgctgtggc tgaggacgat ggcgcgctgc agtatggcgc
     5221 ggtcctcgac agcgatggcg gtgtcggcga gggcgccgaa ccccgtggtc actccatata
     5281 tggaggcgtc ggagtcatgc agccgctcga ccacggccag cgaaggctgc atgctctggc
     5341 gggcagcctc ggccacctcg actcgctcgc cccggcgcgc cacgtcgacg acctggtcga
     5401 tcgtgagcgg cgagccgtcg atgcgcacca gccgacacta cgcggccccg cgcccggagg
     5461 caagcgcgca gagcgatgtc aggcgctagg gttggtccgg atgaagcacg gcacctctgt
     5521 cttcagtcgc caccggctgg ctgtgatcgc ggccttcgtg gtcgtcgcgg tggccgcggg
     5581 aatcccggtg accgcgtatg tgatcgtgcc tctcttcgtg cgttcgaccg tggtcgagag
     5641 agcccccagc accacgccat cggccgcggg cacatccaca cccgacaccg catccacccc
     5701 ggctgtcgcc ggcaacacga cgctttcgac cggcacgctg cagcgaatca gcgctgtcga
     5761 cttcggcacc ggcaaggtgt cgatcctgca ggtcagccgg cggcgcttcc ttcgctttga
     5821 gaatgtcgag atctcggcag caccggcgat ccacgtctat ctgtccgacc gcatcgacgg
     5881 cagccccggg accttcaccg acctgggcgt gctcaaagcc accagcggtt cgttcaacta
     5941 cgtgatccca accacagtcg atctcagcag ggtgaagtca gtggttgcct ggtgccagca
     6001 gttcacggtc acgatcacct acgcggtgct tcagccgacc tagttcgctg cctggaggtt
     6061 ctccaactca aggtggtcgt gcgagcagaa ga
//
LOCUS       DATCQR010000011         9424 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_186505, whole genome shotgun sequence.
ACCESSION   DATCQR010000011 DATCQR010000000
VERSION     DATCQR010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 9424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9424
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186505"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..841)
                     /locus_tag="VIN32_00365"
     CDS             complement(<1..841)
                     /locus_tag="VIN32_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014921831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8921065.1"
                     /translation="MTAPAAHNDAARAVERTYLILTLLSTLASSFIWGINTIFLLDAG
                     LNNAEAFAANAFFTLGMVVFEIPTGVIADTRGRQLSYVLGAATLLVSTLLYLVMWQVH
                     APLWGWAIASIMLGLGFTFFSGATEAWLVDALHATGFQGHLEHVFGRAQTVAGAAMLF
                     GTVAGGLVAQVTNLGVPYIIRAAMLGVTLVVAWRFMHDIGFSPDRDASAVTAVRNVLK
                     GSIDGGLRNPPVRWLMLAAPFIAGTGIYIFYAAQPYLLELYGDPKAYSIAGLAAAIFA
                     GVQI"
     gene            complement(866..1810)
                     /locus_tag="VIN32_00370"
     CDS             complement(866..1810)
                     /locus_tag="VIN32_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921066.1"
                     /translation="MTLRGWRLAFKMQRLELLIFLAVVVLLVAASLLIARGTSEARAA
                     FDACYQSAGSDANCSTSPSLNDFSLWGGFATLGAFLTPFALGLFLGVPVVAREVEGRT
                     APIAWTLSRSRSRWLIQRVLPLAVVVILVSAAVGIGSEFVTRTNPYADYASNPGFADY
                     TSRGVLLPVRAVVVLVLGVAVGAMVPRQLPALLLGAGVTVALFVALTLGMDAWMTSEA
                     EPIPMADVQLGGPRIFDVAYREDATGDVITMNDFYNQHGDVALPDGEQDPPGFTQVAI
                     GIPGDQYWVWAGRESAIMGGLALAGGGLAALVIRRRRP"
     gene            complement(1807..2787)
                     /locus_tag="VIN32_00375"
     CDS             complement(1807..2787)
                     /locus_tag="VIN32_00375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921067.1"
                     /translation="MSRIGSWLSISFRLHRWEVLASMAGVALLTAAMLWFAWQMRMLA
                     GANPGCLDPNSPAPGCTLFAQRFYELSSKAEWLSYFSALAPFAMGLVLGVPLVSREVE
                     QRTAAIAWTLSRSRAWWLAKRVAFLVLVLVVLLTIVAVAGEVLASAQMPNLHLDRDFS
                     WYGRRGVLIVIRGLAALGIGVLVGAMLGRVLPALLVGVFASVLVFSAISLGMDRWLEG
                     DAIVQAFDADRAGGRQLGERILLPSGELVDYGELKRRGLAYEVIQDDLLWAKPEDIGH
                     MDRLIGYDRYLVVPGSLYPQIVLRESAVVGGATILLALAAAAVVRRRRPG"
     gene            complement(2784..3656)
                     /locus_tag="VIN32_00380"
     CDS             complement(2784..3656)
                     /locus_tag="VIN32_00380"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8921068.1"
                     /translation="MSAEPALEAHDLAKRYGRRTWALTGIDLAIPQGGITALVGPNAA
                     GKSTLIKTWVGFERPTRGNVTVAGIDPQRDRSSVLGLVGYVPQSPALYDGLSVEDHLD
                     LAVLLRPDFDRAYARRRLDQLGIPADQGAKSLSGGQQAQVALALALGTRARILLLDEP
                     LASLDPLARREFLHVLSGAVRSDGATALLSSHIVTDVEQACDRLVVLGVGRILLHDTV
                     ADALATHWLATGGAAKPDGEAIGSFGGPSGERLTLLRRSATSAAPDHRPASLEEVVLG
                     YLASGRGSPGEAAA"
     gene            complement(3653..3865)
                     /locus_tag="VIN32_00385"
     CDS             complement(3653..3865)
                     /locus_tag="VIN32_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921069.1"
                     /translation="MTRHPADLLSLLAGLAVLALGLVLLSGGVGDLPMEWVGPAVAIG
                     LGVLIVFAARSNRDPDEDVPAASDES"
     gene            complement(3862..5250)
                     /locus_tag="VIN32_00390"
     CDS             complement(3862..5250)
                     /locus_tag="VIN32_00390"
                     /inference="COORDINATES: protein motif:HMM:NF015957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEY8921070.1"
                     /translation="MTSQRSERTGAPPRILRRRTRDRVLGGVAGGLGDYFNIDPLLLR
                     IGFVGLMIFGGAGLVLYVVAWLLIPADGQDASSVESFIGRLGLTPSRIGWILLAFVVI
                     VLISNMPIGGPLDGSGSVFIGPLPGLNPAALWALGIILVGIVLLRSRESAPAISPTAA
                     SADGLDQSPDEGFMHRLGLMPLRAGVVVLIVGVVSLFLSTVNGADLSLPSFGQTPQAF
                     WAVAIIAIGVLLLRRRGAAPSIVAVEHESHDDVALATVIAPPRPRSPLALYACAAVLL
                     SIGLLAIVSQTMELSVGPGQFFGAALTVIGIGLIVGAWWGRARILILLALLLLPVAVG
                     ASFITTPLEGGVGDHHYVPVTVAELKDEYRSLSGRTILDLQDLQTSPRDIHIAASVAM
                     GQLVVILPEGASVELRTRVGAGDSYVLGSSDGGTSLDNRYIRRHLNQTTYVLDLEVGV
                     GEVFVTNSRVNW"
     gene            5374..6609
                     /locus_tag="VIN32_00395"
     CDS             5374..6609
                     /locus_tag="VIN32_00395"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEY8921071.1"
                     /translation="MPDMAQVGVRRGGGERAGALRPRLERSRTDRVIGGVAAGIGHHL
                     GIEPIVVRISFVVLSLAFGFGLVVYLLAWLLAPLEPADAAFTSRARILIRPTLGQALG
                     GALILGGVLLLLLVSGFWFGETLAWPVSLAAIGFAILWARSGEERGARWPFDAIRGRV
                     SPGRLAIGSLLILGAMVVFLATNTSLRALGSTLLAALVGIGGLALIGGPWAWNLGREL
                     IEERSQRARTEARAEMAAHLHDSVLQTLALIQRSPAQREMVTLARTQERELRTWLYGR
                     APTLAGARLRDAIDAMAGRVERDQQVKVEAIVVGDADLDEHARALVSALGEATLNAAR
                     HSGSTEVSVYVEVEDDAISAYVRDHGTGFDPEAVAADRRGIADSIVGRMERHGGTAGV
                     NSRPGAGTEIVMRLARKPA"
     gene            6606..7265
                     /locus_tag="VIN32_00400"
     CDS             6606..7265
                     /locus_tag="VIN32_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012015030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY8921072.1"
                     /translation="MTAPTRGATGVSVFVVDDHELFRSGVRSELSRTCRIVGDAGTVE
                     DAVAGIIRADPDVVLLDVHMPAGGGRGVIEGVRAAGSGAQFLALSVSDAAEDVIGIIR
                     AGARGYVTKSISADELVAAVQRVHDGDVVFSPRLAGFVLDAFAGESDQPIDPELDLLT
                     ARERETLRFIARGYTYKEVASELHISVKTVETHVSAVLRKLQLSNRYELTRWASDRRL
                     V"
     gene            complement(7291..7683)
                     /locus_tag="VIN32_00405"
     CDS             complement(7291..7683)
                     /locus_tag="VIN32_00405"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY8921073.1"
                     /translation="MNDHPNAALVRKMTESFNSGDVQGMADMVADDVEWHEIGRSEPI
                     IGKEALAARFGMGDGTPPPYQITAETHDVIANDDHTVVLANAHATKDGRTFDYKVAEI
                     YHMKDGKITARWAVSDDTQAINDFFGGE"
     gene            7780..8427
                     /locus_tag="VIN32_00410"
     CDS             7780..8427
                     /locus_tag="VIN32_00410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921074.1"
                     /translation="MTERLGFTVRAAVTTFASLVLAHNLIFIAGYGSRFGSVMAYTGH
                     DHGWTVAAVSSIALAASLLVVGVRRLQQLRRTARGAGLPSLPDEPGLRAFAIRWLGWW
                     IALSLATTLLFAVQENLELGSVGTAMPGLSVLASAAYPNAVAIILAVALGISMVAALL
                     GWRSSVLIARIQAARSETPAAAALSLPPCEPIDRRLGSILGRRLAVRAPPLTLAS"
     gene            8489..9151
                     /locus_tag="VIN32_00415"
     CDS             8489..9151
                     /locus_tag="VIN32_00415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921075.1"
                     /translation="MGKLRARSALAATLAVLLGLLVASVANAHVVKQAGPYTLEIGWQ
                     HEPTYVGEANGVQVIVHDADDQPITDLATDDLKVVVSTADQQSGELTFEPGFDLKEGD
                     GNPGEYNAPIMPTAPGDYTFHLTGAIHGEAVDITVTSSDETFDTVRGTAEIQFPAKVP
                     SLTEVVTRLDRIDARLASADDAKAAADRALLVGAGFGLLGVIVGAVALLVARRGARDA
                     KV"
     gene            9154..>9424
                     /locus_tag="VIN32_00420"
     CDS             9154..>9424
                     /locus_tag="VIN32_00420"
                     /inference="COORDINATES: protein motif:HMM:NF016149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopC"
                     /protein_id="HEY8921076.1"
                     /translation="MIRTTRSVAFLALVLGGALGLPQAVLAHALLISSTPAAGATLSA
                     PPTEVVLTFGEAPDPKLTLVRVLDVSGQDQVDGAVVRVAGEPDQLK"
BASE COUNT         1523 a         3279 c         3236 g         1386 t
ORIGIN      
        1 cgatctgcac gccggcgaag atcgccgccg ccaggccggc gatgctgtag gccttcgggt
       61 ccccgtacag ctccagcagg tagggctgcg cggcgtagaa gatgtagatg ccggtgccgg
      121 ctatgaacgg agcggccagc atcagccagc ggaccggcgg gttgcgcagg cccccgtcga
      181 tcgacccctt gagcacgttg cggacagctg tgaccgcgct tgcgtcgcgg tcgggtgaga
      241 agccgatgtc gtgcatgaag cgccaagcca cgaccagagt cacccccagc atcgccgccc
      301 gaatgatgta gggcacgcca aggttcgtga cctgcgcgac aagcccgccg gcgacggtgc
      361 cgaacagcat cgccgcgccg gccaccgtct gggcgcggcc gaagacatgt tcgaggtgac
      421 cctggaagcc ggtggcgtgc agcgcgtcga ccagccaggc ctcggtcgcg cccgagaaga
      481 aggtgaagcc cagccccagc atgatcgagg cgatcgccca gccccagagc ggcgcgtgga
      541 cctgccacat gacgaggtag aggagcgttg agaccaggag cgtggcggcc cccagcacgt
      601 aggagagctg gcggccgcgc gtgtcagcaa tcacgccggt ggggatctcg aagaccacca
      661 tgccgagggt gaagaaggcg ttggcggcaa acgcctcggc gttgttgagg ccggcatcca
      721 ggaggaagat cgtgttgatg ccccagatga acgacgaggc gagcgtgcta agcagcgtca
      781 ggatcaggta cgtccgctcc accgctcgcg cggcgtcatt gtgggccgcg ggcgccgtca
      841 tgcggggact ctacccgctt gcgccctagg gccgcctccg ccgtatcacc agcgccgcca
      901 gcccgccccc ggcaagcgcc agcccgccca tgatggctga ctcccgcccg gcccagaccc
      961 agtactggtc accggggatg ccgatcgcga cctgggtgaa gcctggcggg tcctgctcgc
     1021 catccggcag ggcgacatcg ccatgctggt tgtagaagtc attcatcgtg atcacgtcgc
     1081 cggtggcatc ctcccggtag gccacgtcga agatgcgagg cccgcccagc tggacatccg
     1141 ccatcgggat cggctctgcc tccgaggtca tccaggcgtc catgccgagc gtcagggcga
     1201 cgaacaacgc caccgtgacg cccgctccca ggaggagcgc cggcagctgg cgcggaacca
     1261 tcgctccgac ggcgacgcca agcaccagca ccacgacggc tcgcacgggc agcagcacgc
     1321 cgcgagaggt gtagtcggcg aagcccgggt tggacgcgta gtctgcatag gggttcgttc
     1381 gtgtcacgaa ctcacttccg atcccaaccg ctgccgagac aaggatcacg acaaccgcca
     1441 gcggaaggac ccgctggatc agccagcgag agcgggaccg actcagcgtc caggcgatcg
     1501 gggcggtccg tccctcgacc tcgcgggcga ccaccgggac tcccaggaac aggcccagcg
     1561 cgaatggcgt caggaaggcc ccgagggttg cgaagccgcc ccatagtgag aagtcattca
     1621 ggctcggcga cgtcgagcag ttggcgtctg acccggccga ctgatagcag gcgtcaaaag
     1681 cggcccgcgc ctcgctcgtc ccccttgcga tcagcagcga tgccgccacc agcaggacca
     1741 ccaccgccag gaagatcagc agctcgagcc gctgcatctt gaacgccagc cgccagccgc
     1801 ggagcgtcat cccggcctcc gacggcggac gacggctgcg gcagccagcg ccagcaggat
     1861 ggtggcgcca cccaccaccg ccgactcgcg caggacgatc tgcgggtaga gcgaccccgg
     1921 caccaccagg taacggtcgt agccgatgag tcgatccatg tggccgatat cctcgggctt
     1981 ggcccagaga aggtcgtcct ggatcacctc gtacgccagc ccgcggcgct tgagctcccc
     2041 gtaatcgacc agctcgccac tgggcaggag gatgcgctcg ccgagctgac gccccccggc
     2101 gcgatccgcg tcgaaggcct ggacgatggc gtctccctcc aaccatcggt ccatgcccag
     2161 gctgatggcg ctgaagacca ggaccgatgc gaagaccccc accaggaggg ccggcaggac
     2221 cctgcccagc attgcgccca caagcacgcc gatcccgagt gccgccagcc cgcggatcac
     2281 gatcaggacg ccgcgacgcc cataccagct gaagtcgcga tccaggtgga ggtttggcat
     2341 ctgggccgag gccagcacct cgccggcgac ggccacgatg gtcagcagga cgaccagcac
     2401 cagcaccagg aaggcgacac gtttcgccag ccaccacgcg cgcgagcggc tcagcgtcca
     2461 cgcgatggcc gcggttcgct gctcgacctc acgcgacacc agcggtacgc cgagcacgag
     2521 ccccatcgcg aacggagcga gcgcgctgaa ataggagagc cactcggcct tgctgctcag
     2581 ctcgtagaag cgctgcgcga acagggtgca acctggagca ggcgagttag ggtcgaggca
     2641 gcctgggttc gccccggcca gcatgcgcat ctgccaggcg aaccagagca ttgcggcggt
     2701 gagcagcgcg actcccgcca tcgaggcgag gacctcccag cggtgcagtc gaaacgagat
     2761 ggacagccag gagccgatac ggctcacgca gccgcctcgc ccgggctccc ccggccggac
     2821 gccaggtagc cgagcacgac ctcctcgagc gaggcggggc gatggtccgg cgcggcggag
     2881 gtcgccgacc ggcgaaggag cgtcaggcgc tcgccggagg gaccgccgaa cgacccgatc
     2941 gcctcgccat cgggctttgc cgctccgccg gtcgcaagcc agtgcgtcgc cagcgcgtcc
     3001 gccacggtgt cgtgcagcag gatgcggccg acccccagca ccaccagccg gtcgcaggcc
     3061 tgctcgacgt cggtgacgat gtggctcgag aggagcgcag tggcgccgtc gctgcgaacc
     3121 gcaccgctca ggacgtgcag gaactcacgg cgcgcgaggg ggtcgaggct ggccagcggc
     3181 tcgtccagga gcaggatccg cgcccgtgtg cccagggcaa gcgccagcgc cacctgcgcc
     3241 tgctgtccgc cggacaggga cttcgcgccc tggtcggcgg gaatgccgag ctgatcgagc
     3301 cgccgccggg cgtaggcccg atcgaagtcc gggcgcaata ggaccgcgag gtcgaggtgg
     3361 tcctccacgc tcagcccgtc gtacagggcc ggcgactgcg ggacgtagcc aaccagcccc
     3421 agcacgctcg agcgatcccg ctgtggatcg atccccgcca cggtcacgtt gccacgcgta
     3481 gggcgctcga agccgaccca tgtcttgatg agcgtcgact tccccgcggc gttcggcccc
     3541 accaacgccg tgatcccgcc ctgcggaatc gccaggtcga tcccggtcag cgcccaggtg
     3601 cgtcgcccgt agcgcttggc aaggtcgtgc gcctcgaggg ccggctcggc gctcatgatt
     3661 cgtcgctcgc cgctggcaca tcctcgtccg gatcgcggtt cgatcgcgcg gcaaacacga
     3721 tcaggacccc gaggccgatc gcgaccgccg ggccgaccca ctccatcggc aggtcgccaa
     3781 ctccaccgga gaggaggacc aggcccaggg ccagcacggc gagccccgcg agcagcgaga
     3841 gcaggtcggc cggatggcgc gtcaccaatt gaccctcgaa ttggtcacga aaacctcgcc
     3901 gacgcccacc tcgaggtcaa gcacgtaggt cgtctggttc aggtgacggc ggatgtagcg
     3961 gttgtccagg ctggtgccgc cgtccgatga gccaaggacg taggaatcgc cagctccgac
     4021 gcgcgtgcgc agctcgaccg acgccccctc gggcaggatg accaccagct gtcccatcgc
     4081 gacgctcgcc gcgatgtgaa tgtcccgcgg gctcgtctgc aggtcctgca ggtcgaggat
     4141 cgtccgccca ctcagggagc ggtactcgtc cttcagctcc gccaccgtga ccgggacgta
     4201 gtgatggtca ccgacgccgc cctcgagtgg cgtcgtgatg aagctggctc cgacggccac
     4261 agggagcagt aagagcgcga gcagaatcag gatccgcgcg cggccccacc aggcgcccac
     4321 gatcagcccg atcccgatga ccgtgagggc tgccccgaag aactgcccag gccccacgct
     4381 gagctccatc gtctggctga cgatggccag cagcccgatg ctcaacagca ccgccgcgca
     4441 ggcgtacagg gccaggggcg agcgtgggcg agggggcgct atcaccgttg ccaaggcgac
     4501 atcatcgtgc gattcgtgct caacggcaac gatcgatggg gccgcgccac ggcggcggag
     4561 cagcaggacg ccgatcgcaa tgattgccac tgcccagaag gcttggggcg tttggccgaa
     4621 tgacgggaga ctgaggtcag ccccattgac ggtgctgagg aagagactca cgaccccgac
     4681 gatcaagacc accacgcctg cccgaagagg catgagcccc aggcgatgca tgaacccctc
     4741 gtccggggac tggtcgaggc catccgcgga ggccgccgtc ggcgagatcg ccggcgcgct
     4801 ctcgcggcta cgaaggagca cgatgccgac caggatgatc cccagcgccc acagcgcggc
     4861 cgggttgagg cccggcagag ggccgatgaa gacgctcccc gagccgtcca gtggtccgcc
     4921 gatcggcatg ttcgagatca ggacgatgac cacgaaggcc agcaggatcc acccgatgcg
     4981 gcttggggtg aggccgagcc ggccgatgaa gctctcgaca ctcgatgcgt cctggccgtc
     5041 ggcagggatc agcagccagg ccaccacgta aaggacgagg cccgctccac cgaagatcat
     5101 cagccccacg aagccgatgc ggaggaggag cggatcgatg ttgaaataat cgccaagccc
     5161 gccggcgacc cctcccagca cccgatcgcg ggtccggcgg cgaaggattc gcggcggggc
     5221 cccggtgcgt tccgaacgtt ggcttgtcat cggcccgatc ttggtgcccg gtgcgatggc
     5281 tgcctatcag ggagcaccct gaaactaccc tgaaaggcgg cctttcccct cagggtcatc
     5341 cccgatcgtc ggtgagccgg ggcatgtgcg aacatgccgg acatggcgca ggttggcgtg
     5401 aggcgaggcg gcggtgagcg ggccggcgcc ctgcggccgc ggcttgagcg cagccgcacc
     5461 gaccgcgtca tcgggggcgt ggctgccggg atcggccatc acctgggcat cgagccgatc
     5521 gtcgtgcgca tctccttcgt ggtcctgagc cttgcctttg ggttcggctt ggtcgtctac
     5581 ctcctcgcct ggctgctcgc accactcgag ccagccgacg ccgcgttcac gtcgcgcgct
     5641 cgcatcctga tccggcccac gctcggtcag gcccttggcg gcgcgctgat cctggggggc
     5701 gtcctgttgc tcctgctcgt ctcgggcttc tggttcggcg agacgctggc ctggcccgtg
     5761 tcgctggccg ccattggctt cgcgatcctg tgggcccgct caggggagga gcgcggcgcg
     5821 cgctggccgt tcgatgcaat ccgcgggcgg gtctcaccgg ggcgacttgc gatcggtagc
     5881 ctcctgatcc tcggtgcgat ggtcgtcttc ctcgccacca acacctcgct ccgcgccttg
     5941 ggcagcaccc tgctggcggc actcgtcggg atcggggggc tagccctgat cggcgggccg
     6001 tgggcgtgga atctgggccg cgagctgatc gaggaacgga gccagcgcgc acggaccgag
     6061 gcgcgtgccg agatggccgc gcacctccac gactcggtgc tgcagaccct ggccctcatc
     6121 cagcgctcgc cggcccagcg cgagatggtg acgctggcgc gcacgcagga gcgcgagctg
     6181 cggacgtggc tgtacggccg cgccccgacc ctcgccggcg cgcggctccg cgacgcgatc
     6241 gatgccatgg ccgggcgggt cgagcgggac cagcaggtca aggtcgaggc gatcgtcgtc
     6301 ggcgacgccg atctcgacga gcacgcccgc gccctcgtct cggcgctcgg cgaggcgacg
     6361 ctcaatgccg cgaggcattc gggctcaacg gaggtatcgg tctatgtgga ggtggaggac
     6421 gacgcgatca gcgcgtatgt gcgagaccat gggaccggct tcgacccgga ggcagtcgcc
     6481 gcggaccggc gcgggatcgc ggactcgatc gtcggccgca tggagcgcca cggcgggacc
     6541 gcgggggtga acagccgccc cggcgcgggg acggagatcg tcatgcgcct tgccaggaag
     6601 ccggcatgac cgcgccaact cgaggcgcca cgggggtcag cgtcttcgtg gtcgacgacc
     6661 atgagctctt tcgctcgggc gttcgttccg agctgagccg cacctgcagg atcgtgggcg
     6721 acgcggggac ggtcgaggat gcggtggcgg gcatcatccg agcggatccc gacgtggtgc
     6781 tgctcgacgt ccacatgccg gccggaggcg gtcgcggggt gatcgagggg gtacgggccg
     6841 cgggctcggg cgcccagttc ctggccctct ccgtctcgga cgccgcggag gacgtgatcg
     6901 gcatcatccg agccggagct cgcggctacg tcaccaagtc catctcggcc gatgagctgg
     6961 ttgccgccgt ccagcgcgtc cacgacgggg atgtcgtctt ctcgccgcgc ctggcaggct
     7021 tcgtgcttga tgcgttcgcc ggcgagtcag accagccgat cgacccggag ctcgacctcc
     7081 tgacggcccg cgagcgcgag accctgcgct tcatcgcgcg cgggtacacc tacaaggagg
     7141 tggcatccga gctgcacatc tcggtcaaga ccgtcgagac gcatgtctcg gccgtgctcc
     7201 gcaagctcca gctttccaac cgatacgagc tgacccgctg ggcctcggac cgccgcctgg
     7261 tctaggtcac gcctcgcacc ggggtgaggg tcattcgccc ccgaagaagt cgttgatcgc
     7321 ctgggtgtcg tccgagaccg cccagcgcgc ggtgatcttt ccgtccttca tgtgatagat
     7381 ctcggcgacc ttgtagtcga aggtgcgccc atctttcgtg gcgtgcgcgt tggccaatac
     7441 gaccgtgtga tcgtcgttgg cgattacgtc gtgcgtctct gccgtaatct gatagggcgg
     7501 aggcgtgccg tcacccattc cgaatcgcgc ggctagagcc tccttgccaa tgatcggctc
     7561 ggagcggcca atctcgtgcc attcgacgtc atcggcgacc atgtcggcca ttccctggac
     7621 atccccgctg ttgaagctct ccgtcatctt gcggaccagg gccgcattgg ggtggtcgtt
     7681 catgggactc ctcctgtggg gaacgtggac cgagatgata gtcgcccgca tccaccctca
     7741 actccgcgcc cgcctctgga ctggcctaga gtaccgatga tgaccgagcg gttggggttc
     7801 acggtccgtg ccgcagtgac gacgttcgcc tcgctggtgc tcgcccacaa cctcatcttc
     7861 atcgccggct acgggtcccg cttcggttcg gtgatggcgt acaccggcca cgatcacggc
     7921 tggaccgtcg ccgccgtcag ctcgatcgcc ctcgccgcct ccctgctggt cgtcggcgtc
     7981 cggcggcttc agcagcttcg gcggactgcc cgcggcgcgg gtctcccgag cctccccgac
     8041 gagcccggtc tgcgggcgtt cgcgattcgc tggctcggct ggtggatcgc gctgagtctt
     8101 gccaccaccc ttctgttcgc cgtgcaggag aatctcgagc tcgggagcgt cggcacggcc
     8161 atgcctgggc tgagcgtcct ggcctccgcg gcctatccga atgccgttgc catcatcttg
     8221 gcggtcgcgc ttgggatatc gatggtggcc gctctgctcg gctggagatc atcggtcctc
     8281 attgcccgga tccaggcggc ccgctcggag acgccggccg cggcagcact gtcattgccg
     8341 ccctgcgagc cgattgaccg tcgcctgggt tcgatcctcg gccgccgact cgccgttcgc
     8401 gctccgccgc tcacgctcgc gtcctgacca aatccgagcc gaggtcgggc ctgcccggat
     8461 tccatcggcc tatgcggagg acacgttcat gggcaaactt cgggcgcgca gcgccctcgc
     8521 cgccacgctt gcggtcctgt tgggccttct cgtggcgagc gtcgcgaacg cgcacgtcgt
     8581 gaagcaggcg ggcccgtaca cgctcgagat cggctggcag cacgagccga catacgtcgg
     8641 cgaggccaac ggcgtgcagg tgatcgtcca cgacgccgat gaccagccga tcaccgacct
     8701 cgccaccgac gatctcaagg tggtggtctc gactgccgac cagcagtcgg gggagctcac
     8761 ctttgagcca ggcttcgacc tgaaggaggg cgacggcaac ccgggcgagt acaacgcgcc
     8821 gatcatgccc accgcgcccg gtgactacac cttccacctc accggcgcaa tccacgggga
     8881 ggcggtcgac atcaccgtga cctcgtccga cgagacgttc gacacggtgc gcgggactgc
     8941 cgagatccag ttccccgcga aggtgccgag cctgaccgag gtggtgacac gccttgaccg
     9001 cattgacgcg cggctggcga gcgccgacga tgccaaggcc gcagcggatc gggcattgct
     9061 ggtgggcgcc gggttcggcc tgctgggcgt gatcgtcggg gcagtggcgc tcctggtggc
     9121 gaggcgcggc gcacgggacg ccaaggtgta gccatgatcc ggaccacgcg ctcggtcgca
     9181 ttcctggcgc tcgttctggg cggggccctg gggctccccc aggccgtgct cgcgcacgcc
     9241 ctgcttatct cctcgacacc ggcggccggc gcaacgctca gcgcgccacc aaccgaggtc
     9301 gtgctcacct tcggggaggc gcccgacccg aagctcaccc tggtgagggt gctcgatgtc
     9361 tcgggccagg atcaggtgga cggagcggtc gtgcgggtgg cgggggagcc ggaccagctg
     9421 aagg
//
LOCUS       DATCQR010000012         7509 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_140197, whole genome shotgun sequence.
ACCESSION   DATCQR010000012 DATCQR010000000
VERSION     DATCQR010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 7509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7509
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140197"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..531)
                     /locus_tag="VIN32_00425"
     CDS             complement(<1..531)
                     /locus_tag="VIN32_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015098014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HEY8921077.1"
                     /translation="MTKATLAVADTATITVGGHSAELPVVRGTAGDDGVDIGDLRAQT
                     GVVTLDNGYRNTGSCASAITYLDGDEGILRYRGYPIEQLAEKSTFLEVAYLLIYGELP
                     TTPQLETFVDSITRHSLIHEEMRRFFDSFPKNAHPMAVLSAGASALSTFYQDSHDPFN
                     PDHVEISTHRLLAKMPT"
     gene            complement(528..1517)
                     /locus_tag="VIN32_00430"
     CDS             complement(528..1517)
                     /locus_tag="VIN32_00430"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HEY8921078.1"
                     /translation="MPDSHMKVAVTGAAGQIGYALLFRIASGQVFGPRTTVDLHLLEL
                     EAALPALEGVAMELEDCAFPLLRRVVRTSSADEAFAGVNWALLVGAVPRKAGMERKDL
                     LTINGGIFSAQGRAIAGNAAPDVRTLVVGNPCNTNCLIAAANAPEIPADRWFAMTMLD
                     ENRGRSQLAARAGVAVDTVTDLAVWGNHSSSQFPDFYHARIGGRPAVEVIDDEGWLQG
                     EFIDTVQQRGAAIIAARGQSSAASAANAVIDTVRNITQPNGEIFSSAIPAPADGGYGV
                     PEGIVFGYPLRATAPGTIEIEPGIVHNAWAQARVDATLAELIEEREAVMELVG"
     gene            complement(1569..1985)
                     /locus_tag="VIN32_00435"
     CDS             complement(1569..1985)
                     /locus_tag="VIN32_00435"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012876586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HEY8921079.1"
                     /translation="MNRRRVLFVCTHNSARSQMAEGMLRAWAGDRFEVASGGTEATGV
                     RPEAIVAMAELGIDIRGHASKTIEQFIGQPWDWLIPVCEEACEACPYVPGAQTVLRWA
                     FDDPSAATGTEQQRLGEFRRVRDELASQVRAFIAAT"
     gene            complement(2187..2918)
                     /gene="upp"
                     /locus_tag="VIN32_00440"
     CDS             complement(2187..2918)
                     /gene="upp"
                     /locus_tag="VIN32_00440"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEY8921080.1"
                     /translation="MDAARFPNLHVSQHPLVAHKLALLRDRGTEPKKFRELVRELSWL
                     LGYEAMADLTTRPITVETPLEPMSGAELEPKVGLIPVLRAGLGMVDAMLELMPSAEVW
                     HIGLYRDERTLKPVEYYNKLPDAATVQVCLILDPMLATGGSSSATVDILKQWGAARVK
                     QVSLIAAPEGVATLSAAHPDVDIHVGAVDRALNERGYIVPGLGDAGDRQFGTFAGPSS
                     EPGHDPADETAEVVRRLQRDLAGEP"
     gene            3055..3696
                     /locus_tag="VIN32_00445"
     CDS             3055..3696
                     /locus_tag="VIN32_00445"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEY8921081.1"
                     /translation="MVFGTKRDAKADSGLREDVVGNLYPDSFPDEAEMTPNPMEFRRP
                     DPGNESVIDPHARFNGKYVSDRDLRIEGEASGEIECSGTLIISPQAKVRSSITAANVI
                     INGDYEGDVDCGGRFEIGSTGRVKGKVKAQVLVVKEGAHFEGAVMMTPEGRASSNAPA
                     PATASAPAKGGGNAQPPQTSAASQGQGSIFNKEREAQSAPDRAASGTGTGSNS"
     gene            3718..4761
                     /locus_tag="VIN32_00450"
     CDS             3718..4761
                     /locus_tag="VIN32_00450"
                     /inference="COORDINATES: protein motif:HMM:NF017864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEY8921082.1"
                     /translation="MARLLVEVTRGDRVESRHRGSIAVVSSSGELSWSFGDADGFAFI
                     RSSAKPFQLAPFVASGRFDAYDFPNPTESLAIMAASHSGEDRHVRTVQAVLRAGGLTR
                     DVLACGVHAPFDTETAQRLIRDGEPLTPLRHNCSGKHAAMALHAKAAGWPIETYWQPD
                     HPVQQLALDTVSLMSGVARSEIVTATDGCGVVSFGLPLRGLALAFARLADPSSVPDPA
                     LRTAFERIRDAMMAHPELVGGDRRRLDTALMRAAPARLVAKTGAEGVQAVGVLAGSDE
                     SASAAGLAIKIEDGDSSNRARNVATCAALHQLGALGDQALERLSEYASPQILDPRGEA
                     AGAVRPAFELVPG"
     gene            4857..>7509
                     /locus_tag="VIN32_00455"
     CDS             4857..>7509
                     /locus_tag="VIN32_00455"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY8921083.1"
                     /translation="MTTEGRPTKAQNLQDEATEGKRRDSPVRRWAALRSPVALVIAIT
                     TCLAVVVGIVVVSRVGDQVARDGESLLARHAAEESIALEELMAGASRDIRLARRNVIF
                     EEALAHTSGQLLPADRLLVETAITYLGDRYQVDEICVIRADGLETARWVGGKGIAPVS
                     DLSADERLNNPAVIPTVPLPDDAFFQSDPYISPDSDRWVVGIATPIVLNTGEHAGILH
                     FEIPIQRFVDELNGAQFGGSTYGMLLDRSGHLLSAPQLADFRSAQGLPNDPRTAPFPM
                     AGASGSASWRHAVATMISGSSGNVSFDEGGFSFRASYRAISGSDRIVAVVSPTNELYA
                     DAQRDRLILAVTVGPLILLIILVSAWFTYRLSGANRRLGSANQLLAVTSRASAELAAI
                     VRAGHDAMMGVDLDGTIATWNDGAEQMYGLAAPEVVGSSVKVLFQPDRAPEVLELLAT
                     AANGEPVQRHETIHRTGPGTAFHASVTVSPIRDERGAVVRAAFVVRDISDRKRLEEEL
                     AHQALHDGLTGLPNRVLFQDRLRHSLDRAQRPRSADAACQAVLFLDLDNFKLINDTMG
                     HPTGDELLVAVAERINASLRPGDTAARLGGDEFTILLENMQEAETRVVADRLLARLRE
                     PFILNGHEAILSASIGIVISESGLDLPEDLMRSADTALYEAKRNGRDRHETFHPAMTA
                     HVWHRLELEADVRRGLAAREFLLHYQPIIELESGKIRELEALVRWQHPERGLLSPGDF
                     IPLCEETGLIVDLGRIVLRSACAQLAAWDASIPGAHDLVIAVNVSPRELREPTFVAEV
                     RAALKETGLVPGRLKLEITETVTLDQGGPGTAALSSLHDLGVHLAVDDFGTGYSSLGY
                     FRKLAVDSLKIDRLFIDGLGREPGDTAI"
BASE COUNT         1190 a         2566 c         2507 g         1246 t
ORIGIN      
        1 cgtcggcatc ttggcgagga gccgatgggt gctgatctcg acgtggtcag ggttgaacgg
       61 gtcgtggctg tcctggtaga aggtcgacag cgccgatgcg cccgccgaca ggaccgccat
      121 cgggtgcgcg ttcttcggga agctgtcgaa gaagcggcgc atctcctcgt ggatcaggga
      181 atgacgggtg atgctgtcca cgaacgtctc gagctggggc gtggtgggca gctcgccata
      241 gatcaggagg taggcgacct ccaggaaggt gctcttctcg gcgagctgct cgatcgggta
      301 gccccggtag cgaaggatcc cctcgtcgcc gtcgaggtag gtgatcgcgc tcgcgcacga
      361 cccggtgttg cgatatccat tgtccagggt cacgaccccg gtctgggctc gcaggtcacc
      421 gatatcgacc ccatcgtcgc cggcagtgcc acgcaccacc gggagctcgg cgctgtgccc
      481 acccaccgtg atggtggcgg tgtcggcgac cgccagcgtc gccttcgtca tccgaccagc
      541 tccatgaccg cctcgcgctc ctcgatcaac tcggccagcg tcgcgtcgac ccgggcctgt
      601 gcccatgcat tgtggacgat cccgggctcg atctcgatgg tgccgggcgc ggtagcccgg
      661 agcggatagc cgaagacgat cccctccgga acgccgtatc cgccgtccgc gggcgcgggg
      721 atggcggagc tgaagatctc cccgttcggc tgggtgatgt ttcgaaccgt gtcgatcacg
      781 gcgttggcgg cgctggccgc gctggactgg ccgcgcgccg cgatgatcgc cgcgccgcgc
      841 tgctgcaccg tgtcgatgaa ctcaccctgc agccagccct cgtcgtcgat cacctccacg
      901 gccggccgac caccgatccg cgcgtgatag aagtcgggga actggctcga ggagtggttg
      961 ccccagacgg cgaggtcggt caccgtatcg accgcgacgc ccgctcgcgc agccagctgg
     1021 gaccggccgc ggttctcgtc gagcatggtc atcgcgaacc atcgatcggc gggaatctcc
     1081 ggggcattcg cggcggcgat caggcagttg gtgttgcagg gattgccgac caccagggtc
     1141 cgcacgtccg gggcggcgtt ccccgcgatc gccctgccct gggccgagaa gatgcccccg
     1201 ttgatggtga gcaggtcctt gcgctccatc ccagccttgc gaggcacggc ccccaccagc
     1261 agggcccagt tgactccggc gaaggcctcg tccgcgctgg aggtccggac cacgcgtcgc
     1321 agcagcggga aggcgcagtc ctcgagctcc atggccactc cctcgagggc cggcagggcg
     1381 gcctcgagct ccaacaggtg cagatcgacg gttgtgcgcg ggccgaagac ctgcccggag
     1441 gcgatccgga agaggagggc atagccgatc tgtccggcgg cgccggtgac ggccaccttc
     1501 atgtgcgagt cgggcatcac gatcgagtct agcgcagcgc ccccttgccg cacccgcgcc
     1561 gcccaggatc aggtcgcggc gatgaaggcg cgaacctggc tcgccagctc atcgcgcacg
     1621 cggcgaaact cgccaagccg ctgttgctcg gtgcccgtcg cggccgacgg atcgtcgaag
     1681 gcccagcgca ggacggtctg tgcaccgggg acgtagggac acgcctcgca cgcctcctcg
     1741 cagacaggaa tcagccagtc ccaaggctgt ccaatgaact gctcgatcgt cttggaggca
     1801 tgcccgcgga tgtcgatccc cagctcggcc atggcgacga tcgcctcggg gcgaacgccg
     1861 gtcgcctcgg tcccgccgct ggcgacctcg aatcggtcgc cggcccacgc tcgcagcatc
     1921 ccctccgcca tctgcgagcg ggccgagttg tgagtgcaca cgaagaggac gcggcgcctg
     1981 ttcatgaggg cagcgtagcg ggcgactcgg ggatcggctg tggacaagtt gtgaaagatc
     2041 cttgaagttc ggggataagt gggattgcca tcctcgccgc ccggcgccta ttctctggtt
     2101 gtcccgaagg ggcagccaag ggcacccgag attgcatcaa cggaccggcg gcggttcctt
     2161 cgggacattt cgttgcccgg aaacggtcac ggctcgccgg cgaggtcgcg ctgcaggcgc
     2221 cggaccactt ccgcggtctc gtcggcggga tcgtggcccg gctccgacga gggtcccgcg
     2281 aacgtcccga actgccggtc cccggcgtca cccagcccgg gcacgatgta cccacgctca
     2341 ttgagcgccc gatcgacggc ccccacgtgg atgtcgacat cgggatgtgc ggccgagagc
     2401 gtggccaccc cctccggcgc cgcgatcagc gagacctgct tgacgcgcgc agcgccccac
     2461 tgcttgagga tgtcgaccgt ggccgacgac gagccgccgg tcgccagcat cgggtccagg
     2521 atgaggcaga cctgcaccgt cgccgcatcg ggcagcttgt tgtagtactc gaccggcttg
     2581 agggtgcgct catcgcggta caggccgatg tgccacacct cggcgctcgg catcagctcg
     2641 agcatggcgt ccaccattcc cagcccggct cgcagcacgg ggatcagccc gaccttcggc
     2701 tcgagctccg cgcctgacat cggctccagc ggtgtctcga ccgtgatcgg ccgggttgtc
     2761 aggtcggcca tcgcctcgta gccgagcagc catgagagct cgcgtaccag ctcacggaac
     2821 ttctttggtt ccgtgccccg gtcacgcagc agcgccagct tgtgggcgac cagcggatgc
     2881 tgtgagacgt gcagattcgg gaagcgggcg gcatccatcg ggcgaattct aggcggcacc
     2941 cggattgccc actacctcga tttccctttg gctggtaggc tatccgtcct tgagccgata
     3001 cgtcgcacct gtgccggcac gttgtgctaa gcgacgcaga ggaaggagcc agcagtggtc
     3061 ttcggaacca agcgagacgc caaggccgac tctggcctcc gcgaggacgt tgtcggcaac
     3121 ctgtatccgg atagctttcc cgacgaggca gagatgacgc ccaacccgat ggagttccgc
     3181 cgccccgatc cgggcaacga gagcgtcatc gatccccacg cccgcttcaa cggcaagtac
     3241 gtctcggacc gcgacctgcg gatcgagggt gaggcgagcg gcgagatcga gtgctccggc
     3301 accctgatca tcagccccca ggccaaggtt cgcagctcga tcactgccgc gaacgtgatc
     3361 atcaacggcg actacgaggg ggacgtcgac tgtggcggcc gcttcgagat cggctcgacc
     3421 ggccgggtca agggcaaggt caaggcacag gtcctggtcg tcaaggaggg cgcccacttc
     3481 gagggtgccg tgatgatgac gccggagggc cgcgcctcat cgaacgcccc tgcgccggca
     3541 accgcgagtg cgcctgccaa gggcgggggc aacgcacagc ccccgcagac atcggccgcg
     3601 tcgcagggcc agggcagcat cttcaacaag gagcgcgagg ctcagtccgc gcccgaccgg
     3661 gccgcctccg gcaccggcac cggcagcaac tcctgacccg ataggtcacg cgcccgggtg
     3721 gcccgcctcc tggtcgaggt cactcgcggc gatagggtcg agtcgcgcca ccgcggctcc
     3781 atcgcggtcg tctcgtcatc gggggagctc tcgtggtcct tcggtgacgc tgacgggttc
     3841 gccttcatcc ggtcctccgc caagccgttt cagctggctc ccttcgtcgc ctcgggccga
     3901 ttcgacgcct acgacttccc gaatccgacg gagtccctgg cgatcatggc cgcctcgcat
     3961 tccggagagg accgccacgt gcgaacggtg caggccgtcc tgcgcgccgg cgggctgacg
     4021 cgtgacgtgc tggcatgcgg cgttcacgcg ccgttcgaca cggagacggc ccagcgcctg
     4081 attcgcgacg gcgagccact gacaccgctg cgccacaact gctcgggcaa gcacgccgcc
     4141 atggcgctcc acgccaaggc cgccggttgg ccgatcgaga cgtactggca gcccgatcac
     4201 ccggtgcagc agctggcgct cgacacggtg tcactcatga gcggcgtggc ccgatccgag
     4261 atcgttaccg ccaccgatgg ctgtggcgtc gtgtcgttcg gcttgccact gcgcggcctg
     4321 gcgctggcct ttgcccgcct tgcggatccc tcgtccgtcc cggatccggc gctgcgcacg
     4381 gcattcgagc ggatccgcga cgcgatgatg gcgcacccgg agctcgtcgg cggtgatcgg
     4441 cggcggctgg ataccgcgct gatgcgcgcc gcgcccgccc ggctcgtggc caagaccggc
     4501 gctgaggggg tccaggcagt cggtgtgctg gccggatccg acgaatctgc ttcggcagcg
     4561 gggcttgcga tcaagatcga ggacggcgat tcgtcgaacc gggcgcgcaa cgtggcgacc
     4621 tgcgcggcgc tgcaccagct cggcgccttg ggggatcagg ctctcgagcg cctgtctgag
     4681 tacgcctctc cccagatact cgatccacga ggcgaggccg cgggggcggt acggcccgca
     4741 ttcgagcttg tgcctgggta gcctcgcgcc gcattccgca cggccaattg gcggcgggag
     4801 ggggtaggac ctgcgtccca tggccctcct cgcgaatcaa gcctacgttc ctcgccatga
     4861 cgaccgaggg tcgtccaacc aaggcgcaga acttgcagga cgaggccact gaggggaaac
     4921 gacgagactc accagtccgc cggtgggccg cgctacgctc gccggttgcc ctcgtcatcg
     4981 cgatcaccac ctgcctcgca gtcgtcgtcg gcatcgtggt tgtctccagg gttggcgacc
     5041 aggtggcccg agacggagaa tccctgctcg cgcggcacgc ggctgaagag tcaattgcgc
     5101 tggaagagct catggccggc gcctcccgcg atattcgcct cgcgcgacgg aacgtcatct
     5161 tcgaggaggc cctggcgcac acttctggac aattgctgcc ggcggaccgc ctgctcgttg
     5221 aaacggccat cacctatctg ggcgaccgct atcaggtcga cgagatctgc gtcatccgag
     5281 ccgacgggct cgagacagct cggtgggtgg gcggcaaggg cattgctccg gtcagcgacc
     5341 tgtctgccga cgagcgcctg aacaacccgg ccgtcattcc gaccgtgcct ctccccgatg
     5401 acgcgttctt ccagtccgat ccatacatct cccccgactc ggaccgatgg gttgtcggca
     5461 ttgcaacgcc gatcgttctc aataccggtg agcatgccgg catcctccat ttcgagatcc
     5521 cgatccagcg attcgtggac gagctcaacg gagctcagtt cggtggctcc acctatggga
     5581 tgttgctcga ccgctcgggt caccttctgt cggccccgca gctggccgac tttcgatcgg
     5641 cgcagggcct tccgaacgat ccgaggaccg cgccgttccc aatggccggc gcgtctggct
     5701 ctgcctcatg gcggcacgcg gtcgcaacca tgatcagcgg gtcgtcgggc aatgtctcct
     5761 tcgatgaggg cgggttctca ttccgggcaa gctatcgtgc gatctccggc tcagaccgga
     5821 tcgtggccgt cgtcagcccg accaatgagt tgtatgcaga cgcgcagagg gatcggctga
     5881 tcctggcggt cactgtaggg cccctcatcc tgctgatcat cctcgtgagc gcgtggttca
     5941 cctaccgact atcaggcgca aaccgccgcc tgggcagtgc gaaccagctc ctcgccgtaa
     6001 cgagccgtgc gagcgcggag ctggcggcga tcgtgcgggc cggacacgac gcgatgatgg
     6061 gcgtggacct cgatgggacg atcgccacct ggaatgacgg agccgagcag atgtatgggc
     6121 tggcggctcc agaagtggtc ggctcatcgg tgaaggtcct gttccagccc gatcgcgctc
     6181 ccgaggtcct cgagctgctc gccacggcgg cgaacggaga gccggtccag cgccacgaga
     6241 ccattcatcg gaccggccct gggacggcat ttcatgcctc ggtgaccgtc tcgccgatcc
     6301 gcgatgagcg cggagccgtc gtgcgtgcag cgtttgtggt gcgcgacatc tcggaccgaa
     6361 agcgactcga ggaggagctc gcccaccagg ccctgcatga tgggctcacg ggactcccca
     6421 accgcgtcct cttccaggac aggcttcgtc actcgctcga tcgcgcgcag cgcccacgct
     6481 cggcagacgc agcgtgccag gccgtcctgt tcctcgacct cgacaacttc aagctcatca
     6541 acgacaccat gggtcacccg acgggtgatg agctattggt ggccgtagcc gaacgcatca
     6601 acgcgtctct gcgacccggc gacacggccg cgaggcttgg cggcgatgag ttcacgatcc
     6661 tcctggagaa catgcaggag gctgagacgc gcgtggttgc cgaccggctc ctcgcccgtc
     6721 tccgcgaacc tttcatcctc aacggccacg aggccatcct ctcggcgagc atcggaatcg
     6781 tcatcagtga gtccggattg gatctgccgg aggatctgat gcgaagcgcc gacacggcgc
     6841 tgtacgaggc caagagaaac gggcgggacc gccatgagac cttccacccc gccatgacgg
     6901 cacatgtctg gcatcggttg gagctcgagg cggatgtgcg ccgcgggctg gccgcgcgtg
     6961 agttcctgct ccattaccaa ccgatcatcg aactcgagag tggcaagatc cgcgagctgg
     7021 aggcgctggt ccgttggcag caccccgagc gcgggctcct gtcgcccggc gatttcattc
     7081 ccctctgcga agagacgggg ctgatcgtcg atctcgggag gatcgtcctc aggtcagcgt
     7141 gcgctcaact cgcggcctgg gatgcctcga tccccggcgc ccatgacctg gtcatcgcgg
     7201 tgaatgtctc gccccgcgag ctccgcgagc ccacctttgt cgcggaggtt cgtgcggccc
     7261 tgaaggagac cggcctggtg ccagggcggc tgaagctcga gatcaccgag acggtgaccc
     7321 tggaccaggg tggtccggga acggccgcgt tgtccagctt gcatgacctc ggcgtgcacc
     7381 ttgccgtcga cgacttcggc accggctact cgtcgctcgg ttacttccgg aagctcgcgg
     7441 tcgactccct caagatcgac cggctcttca tcgacggcct cgggcgcgag cccggcgata
     7501 cggccatcg
//
LOCUS       DATCQR010000013         3029 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_217488, whole genome shotgun sequence.
ACCESSION   DATCQR010000013 DATCQR010000000
VERSION     DATCQR010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3029
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217488"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(22..501)
                     /locus_tag="VIN32_00460"
     CDS             complement(22..501)
                     /locus_tag="VIN32_00460"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HEY8921084.1"
                     /translation="MARIILFGSAVVGVLLVTLGFAVWSGQYEAEQPSPSAVASGGSL
                     VPGGSSSPAASVVASASAQAQPSAVASGAAATVDIATLKFGPNVTVAAGTTVTWKNGD
                     TVPHTATEGSNGVKAPNARFDVQLPVGASASFTFTAPGTYQVTCTLHPTMNMTVTVQ"
     gene            complement(520..1803)
                     /locus_tag="VIN32_00465"
     CDS             complement(520..1803)
                     /locus_tag="VIN32_00465"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8921085.1"
                     /translation="MLRVLRQRDYALLWFGGFVSQLGDFVLFVGLPYEVYRRTGSTLA
                     TAGMVLAFLIPEILLGSVAGVYVDRWDRRRLMIAVNLIQAVVLLPLLLVGQLGLWVIY
                     AVLVVESSVSQLFGPARVALVPALLRGGKDELLTANATTGVGHHLARLIGPAVGGVIV
                     ATGGLGAVAVVDSVSFVASAGMLSLIRARPGAPRRTESLEHAALSAWRRLVHEWREGL
                     RVVWHQPVLRALLVFATITAIGEGLTGTLFVVWTTEALHSDATGYGWVLSTQAIGGLA
                     GALVIARLGARLRPLPLLIGSALAFGAIDLVLFTYPVIYPYIGPALVMLVIVGVPGAG
                     MGAATTTLQQAETHDSHRGRVVGAIGAVASIGALIGAMSAGILGEFLPVILLLVVQGS
                     GYLIGGTAVALLVGRRRLGADKEGIVGVGSAGGDG"
     gene            complement(1827..2288)
                     /locus_tag="VIN32_00470"
     CDS             complement(1827..2288)
                     /locus_tag="VIN32_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921086.1"
                     /translation="MRIFVFLHIATMFTAVAVTIGPWLLLRRVGRTRDVPAIRRTFAM
                     ALPITRFIPPLYVLGAALGIVAIFTNGFDPFQPFLLIAYALFILASVVGAVVSEPWLK
                     RVAQTASESPAEAPSPELSTALDDPRIRVIDWFDPLIILAFIFDMVVKPFG"
     gene            complement(2363..2725)
                     /locus_tag="VIN32_00475"
     CDS             complement(2363..2725)
                     /locus_tag="VIN32_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018953834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HEY8921087.1"
                     /translation="MADRDPTYQSDCLFCRIVAGEIPADIVFEDDIVVVFRDINPKAP
                     THVLAIPRRHLASVADLTDADGDLLAALFGALRRVAEEAGLKGYRILTNVGEEAGQSV
                     FHLHFHLLGGRPMAWPPG"
     gene            complement(2735..>3029)
                     /locus_tag="VIN32_00480"
     CDS             complement(2735..>3029)
                     /locus_tag="VIN32_00480"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HEY8921088.1"
                     /translation="RGIVPPVDEPVSFAAALEAAGPGSVLLFERHEGKRLTEMEPPSS
                     VFIGPEGGFSPAEVGAAERAGLALAGLGPRILRSETVAVAVAAAILSRTGDFA"
BASE COUNT          544 a         1061 c         1008 g          416 t
ORIGIN      
        1 atcgaggttc ttcagctgca tctactgcac cgtgacggtc atgttcatgg tggggtgcaa
       61 cgtgcaggtc acctggtacg tgcccggggc ggtgaaggtg aagcttgcgg acgccccgac
      121 cggcagctgg acgtcgaagc gtgcattggg cgccttgacc ccgttcgacc cctcggtggc
      181 ggtgtgcggg acggtgtcgc cattcttcca cgtgacggtc gtgccggccg ctaccgtgac
      241 attgggcccg aatttcaggg tcgcgatgtc caccgtcgcc gccgcgccac tggccaccgc
      301 gcttggctgc gcctgggccg atgcgctggc caccacactg gcggcgggac tcgaagaccc
      361 gcccggcacc agactgccac ccgaggcaac ggccgatggg ctcggctgct cagcctcgta
      421 ctggccactc cagaccgcga acccgagcgt caccagcagg acgccaacca ccgcggagcc
      481 gaacaggatg atcctcgcca tcagtcgttg ttcctcgtcc tacccgtcgc caccggcgga
      541 gcctacaccc acgattccct ccttatccgc gccaagtcgc cgtctcccga cgaggagggc
      601 cacggccgtt ccgccgatga ggtagccact cccctgcacc accaggagca ggatgacggg
      661 caggaattct ccgaggatgc cggcactcat cgccccgatc agtgcaccaa tgctggcgac
      721 tgcgccgatg gctcccacaa ctcgcccacg gtggctgtca tgtgtctcgg cctgctggag
      781 cgtggtggtt gcggcaccca tgcccgcgcc cggaacgccg acgatcacca gcatcaccag
      841 ggccggcccg atgtacgggt agatcaccgg gtacgtgaag agcaccaggt caatggcccc
      901 gaaggccagg gcgctcccga tcagcagcgg caacggtctc agcctcgcgc ccaggcgcgc
      961 aatgaccagt gcgcccgcca gcccgccgat cgcctgggtg gacaggaccc agccgtatcc
     1021 ggtcgcgtcc gaatgcagcg cctcggtcgt ccacaccacg aagagggtgc cggtcaggcc
     1081 ctccccgatg gccgtgatgg tggcaaagac gagcagcgcg cggagcacgg gctggtgcca
     1141 gaccacacgc agcccctctc gccattcgtg caccaggcgc ctccaggcgg agagtgccgc
     1201 gtgctccagc gactcggtgc gtcgcggcgc ccccggtcgt gcgcggatga gcgaaagcat
     1261 ccctgccgag gcgacgaacg agacggagtc gaccacggcc accgcaccga ggccgccagt
     1321 cgccacgatc acgcccccga ctgccggacc gatcaggcgc gccaggtgat gccccactcc
     1381 ggtggttgcg ttggcggtca gcagctcgtc cttcccgccc cgcagcagcg ccgggaccag
     1441 ggccacgcgg gccggaccga agagctgcga gacgctgctc tcgaccacga gcaccgcgta
     1501 gatgacccac aggcccagct ggccgaccag caggagcggc agcagcacga cggcctggat
     1561 gaggttgacg gcgatcatca ggcgccgccg atcccatcgg tccacgtaga cccccgccac
     1621 cgagccaagg aggatctccg ggatgaggaa ggccagcacc atccccgccg tggcgagggt
     1681 cgagccagtc cgacgataga cctcgtacgg cagcccaacg aacagcacga agtcgccgag
     1741 ctgggagacg aagccaccga accacaggag tgcgtagtcg cgctggcgaa ggacgcggag
     1801 catcagccgg tcagtcgatc agccgatcag ccgaacggct tcacgaccat gtcgaagatg
     1861 aaggccagga tgatcagcgg atcgaaccag tcgatgacgc gaattctcgg gtcgtcgagc
     1921 gccgtgctca gctccggcga cggtgcctca gccgggctct cggaggcggt ctgcgccacc
     1981 cgcttgagcc agggttcgct caccacagcg cccacaacac tggcgaggat gaacagggca
     2041 tatgcgatga ggaggaacgg ctgaaacggg tcgaagccgt tcgtgaagat ggccacgatc
     2101 cccagcgctg cgcccaggac atacagcggg gggatgaacc tcgtgatcgg cagggccatg
     2161 gcgaaggtgc gccggatcgc cggcacgtcg cgcgtgcgac cgacccgcct gagcaacagc
     2221 cacggaccga tggtcacggc cactgccgtg aacatggttg cgatgtgcag gaagacgaag
     2281 attcgcacgg gcacaggctc cttcggaatg gatgacgggg gcgccattgt ggcgcatcgc
     2341 cgggcgcgaa ttccggtgtt ctctaccccg gcggccaggc catcgggcgg ccaccaagca
     2401 ggtggaagtg gagatggaag acgctctgcc cagcctcctc tccgacgttg gtcaggatcc
     2461 ggtagccctt caggcccgcc tcctcggcga cgcgccgcag cgcgccgaag agcgccgcca
     2521 gcaggtcgcc atcggcatcg gtcaggtctg cgaccgaggc gaggtggcgg cgcgggatgg
     2581 ccagcacgtg cgtcggcgcc tttggattga tgtcgcgaaa cacgaccacg atatcgtctt
     2641 cgaagacgat atcggccggg atctcgccgg ccacgatccg acagaagagg cagtcggact
     2701 ggtaggtggg gtcgcgatcg gccatgcgcg gagcctaggc gaagtcgccg gtccgcgaca
     2761 agatggccgc cgcaaccgcg acggccacgg tctccgagcg caggatccgc ggccccaggc
     2821 cggcgagcgc cagacccgcc cgttccgcgg ctcccacttc cgccgggctg aagcctccct
     2881 ccggaccgat gaagacgctc gacggcggct ccatttcggt cagtcgcttt ccctcgtgcc
     2941 gctcgaagag caggaccgat ccggggccgg ccgcctcgag cgctgctgcg aatgacaccg
     3001 gctcgtcgac aggcggcacg atccctcgc
//
LOCUS       DATCQR010000014         8416 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_109255, whole genome shotgun sequence.
ACCESSION   DATCQR010000014 DATCQR010000000
VERSION     DATCQR010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 8416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8416
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109255"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1862
                     /locus_tag="VIN32_00485"
     CDS             <1..1862
                     /locus_tag="VIN32_00485"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEY8921089.1"
                     /translation="AQGRHKVWGSRPLWVPPQTSTIASHLPKAMGLAFSLARARRLGV
                     ATDLPDDAIVLCSFGDATVNHATALASINTARYAVRLGLPMPILFVCEDNETGISVPT
                     PEGWIASSFGHLEHLRYDLAEGEIDEVWDTVAEAVRFVRGARRPAFLHLHTVRLWGHA
                     GSDAEQSYRSAEEIAENEARDPLLRNARHLIETGAATPEELRSMLADTRERVMAAAEE
                     ATRRPRLETTEEVVAPMAPYHPVRIVARAAKPLLDPAARLELHGGTLPEEALIPTART
                     LAGCLNAALADELGRRPEVILFGEDVGRKGGVYNVTNGLQRRFGTGRVFDTLLDETSI
                     LGIAQGAAHIGLLPIAEIQYLAYLHNALDQLRGEAATLQFFSSGQFRNPMVVRVPGLA
                     YQKGFGGHFHNDNSVGALRDIPGLVVAIPARGDDAARMLRGAVAMAAEDGRVVVFLEP
                     IALYHEKDLETDGDGGWLTDYPAPPATLLPGEVGIYGPLDTGTADLLIVSYGNGLRLS
                     LQAARRLREEHEIAARVLDIRWLNPLPHQAIREQAANIGAVLVVDECRRTGGGVADAI
                     LADLAEAGIGRRLWSVRAVDSFVPLGTATSAVLIGTDQIVAGALTTVSGRLHA"
     gene            1859..2878
                     /locus_tag="VIN32_00490"
     CDS             1859..2878
                     /locus_tag="VIN32_00490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEY8921090.1"
                     /translation="MMQRAVLACPGRGSYAAASLGSLQPDHPWVLRAEQLRAGYGLEP
                     LLDLDRADRFDPSRHLQPIHASPLIFLVSMLDAEAAVADHQVTAVIGNSLGWYTALAA
                     AGALPFDDAFRLVQEMAILQQEPLPAGGPGGQVIYPLADAAWRPDPQLQAAVAAALAE
                     PSVNGDGHVHESIDLGAYAVLAGDEAGVERLLGRLAPVRVGERLFPLRLSQHGPYHTP
                     LVAHVAAAALDQLGDLEWRAPSATIIDGRGVRWTPWSTDPAALRDYTLGEQVTTPFRF
                     AVSVRVALREEAPDVLVLPGPGNSLGGICGQLVVAEGYRGIRSREDFETVQRSETPIV
                     LSMHR"
     gene            2882..3781
                     /locus_tag="VIN32_00495"
     CDS             2882..3781
                     /locus_tag="VIN32_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014136070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HEY8921091.1"
                     /translation="MAEPDASGFVSLPAPINLALTLGPIAHGRGDPTIRFDPDGIWRA
                     TRTPEGPATLRLRAAKGGVQVSAWGAGAANAVGGASDLVGANDDPAVLVAEHRLIGEL
                     AHRFPGLRLPRTNRPFEALLPAICEQKVAGVEARAAFRGIIASHGEPAPGPAGLRLPP
                     SPATLAALPYFAFHRFGLERRRADLIRHAAQLAARIEAAPVAEAYARLAAVPGIGPWT
                     LAEVGRIAFGDPDAVSVGDYHLPNVVAWALAREPRADDARMLELLEPYRGQRGRVQRL
                     LEASGIRAPRHGPRMAPQRIAEI"
     gene            complement(3792..3868)
                     /locus_tag="VIN32_00500"
     tRNA            complement(3792..3868)
                     /locus_tag="VIN32_00500"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3832..3834),aa:Arg,seq:tct)
     gene            3931..4230
                     /locus_tag="VIN32_00505"
     CDS             3931..4230
                     /locus_tag="VIN32_00505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921092.1"
                     /translation="MPDDRIIAPVQLQPTQIETDQQLLRDEDLLNEEHLRRMDQVLLR
                     RNLDHARMFSRKLSLELQQFVHLYTPEANELRRRIEKYMIMERLIEREMARRHSY"
     gene            complement(4378..4695)
                     /locus_tag="VIN32_00510"
     CDS             complement(4378..4695)
                     /locus_tag="VIN32_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012881641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921093.1"
                     /translation="MLQRLMPSSAAPMPGDPPADPPPPDVAEFIRFCHDRKPVGWPEL
                     YDEMCAVAARREFNGWDQDQFAARGLTFSLFEMSRLSGWVRAVLAVPAEPIEGVAVPL
                     MPG"
     gene            4929..5822
                     /locus_tag="VIN32_00515"
     CDS             4929..5822
                     /locus_tag="VIN32_00515"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03560 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEY8921094.1"
                     /translation="MEIGVVVPQGWTGEYDGWEARRAWERTLAVTRQAERLGFESVWV
                     FDHFQTEPEPTDELTFESFTTLAALAASTERVRLGHVVICTAYRNPALTAKMIGTLDV
                     ISGGRMDLGIGAGWKEDEWRAYGYGFPDTKTRLATLADHLEVITRMLGPGRATFAGRY
                     ASVTDAINVPRGLQEPRVPVMVGGNGPKVTWRLAARFADELNLDWLTPNEVGEAKPVI
                     ASRCEEIGRDPSTLRLSVNISRYVLKDAGPSRVELLAGYREVGVHRVMGLIQETADSD
                     EPLESLAADATAAGCELHQSA"
     gene            complement(5840..>8416)
                     /locus_tag="VIN32_00520"
     CDS             complement(5840..>8416)
                     /locus_tag="VIN32_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921095.1"
                     /translation="HQGRSPAYGEGISFWALAEMVRGRAGINESEDPAAARSKVTGML
                     DEWVTDETERRWVEPRMLQLLGLEATEADERPDRESLFAAWRVFFERVAERGVVVLVF
                     EDLQWADDGLLDFIDHILEWSRDRPIYLISLARPELLDRRSDWGAGRRNFTSLVLEPL
                     EPELMRELLAELVPGLPSSVVERILERAEGIPLYAVETVRMLLSDGLVTLQDGIYRPT
                     GDLSELSVPASLHALIASRLDGLDPADRTLLQAASVIGKTFSIDALAAVSGLPTDEVV
                     TRLRALVRREMLSLEADPRSPEQGQYLFVQGLIREVAYGTLAKRDRRRLHIAAARYFE
                     ALDDEGIAGALAEHYLAAYKAQPEGPEGDAVAAQARVALRGAAERARSLGSFLQATRF
                     LEQALEVTTDPVEEAQLHAAAGNAGLYAGLIDEPMAHMARWLQLAQVAGDRRGLMEAI
                     TGNSLALSAHGRIAECVAILEPAFDEYRDLAETREYVRLAAELARAYLLLGRGEDAVK
                     VVDGTLPTAERLELTRETIELLVTRGPALAGMGRLREAIVTLVGAVEASTTYGLADVG
                     LRARVNLSYAAAAEDPELAYRVAREGVELTRHLGMRGYAFYLLSNAAELAIRIGDWDW
                     LLPELEEAVQLETDTASRMRLAEIRGLRGVDVNDEFQWLADRMADMTEVQAQASVDEV
                     HALVALGLGDHRAALDLARRSYQMSIAPDSASLQTAGRAAAWIGDRQALTDALALIGN
                     QTGRVASAMGREAEAALAALEGRPEALAGFAEAIRRWRELGLDFEAALCGLNLLIMLG
                     PSEPAARAVGEEVAQVFERLGALPFQKLLADAMNASVSTPSSRPEAPPVQEVRASATR
                     SE"
BASE COUNT         1314 a         2977 c         2895 g         1230 t
ORIGIN      
        1 tcgcgcaggg acgacacaag gtgtggggca gccggcccct gtgggtccca ccccagacgt
       61 ccacaatcgc aagccatctg cccaaggcga tggggctcgc cttcagcctg gcgagggcgc
      121 ggcggctcgg ggtggcgacc gacctaccgg acgacgcaat cgtgctctgc tcattcggtg
      181 acgcaacggt caaccatgcc acggcactgg cgtcgatcaa caccgcccgc tatgccgtgc
      241 gactcggcct gccgatgccg atcctgttcg tctgcgagga caacgagacc ggcatcagcg
      301 tgccgacgcc cgagggctgg atcgcctcga gcttcggcca cctcgagcac cttcgctacg
      361 acctggccga gggggagatc gacgaggtct gggacacggt cgcggaggcg gtgcgtttcg
      421 tgcgcggcgc ccgccgaccc gccttcctcc accttcacac ggtcaggctg tgggggcatg
      481 ccggcagcga cgccgagcag tcctatcggt ccgcggagga gatcgccgag aacgaggcgc
      541 gggatccgct gctgcgcaac gcccggcacc tgatcgagac cggtgccgca acgccggagg
      601 agctccgatc catgctggcg gacacccggg agcgcgtcat ggcagccgcc gaggaggcga
      661 cccgccggcc acgcctggag accaccgagg aggtcgtcgc gccgatggcg ccctatcacc
      721 cggtccgcat cgtggcgcgt gcggccaagc cactgctcga cccggcggca cgcctcgagc
      781 ttcacggcgg aacgctgccc gaggaggccc tgatcccgac ggcgcggacg ctcgccggtt
      841 gcctgaatgc cgcgctggcg gatgagctgg gaaggcgtcc cgaggtgatc ctgttcgggg
      901 aggatgtcgg gcgcaagggc ggcgtctaca acgtgaccaa cgggctccag cgccgattcg
      961 ggaccggtcg cgtcttcgac accctgttgg acgagacctc gatccttggc atcgcccagg
     1021 gagctgcgca catcgggctc ctgccgatcg ccgagatcca gtacctggcc tacctgcaca
     1081 acgcgctcga tcagctgcgc ggtgaggcgg cgaccctcca gttcttcagc tcgggccagt
     1141 tccgcaaccc gatggtggtc cgcgtccccg gcctcgccta ccagaagggc ttcggtggcc
     1201 acttccacaa cgacaacagc gtcggggccc tgcgcgacat ccccggcctg gtggtggcga
     1261 tcccggcgcg tggcgacgat gcggcgagga tgctgcgtgg cgctgtcgcc atggcggccg
     1321 aggacggacg ggtggtcgtc ttcctggagc cgatcgcgct ctaccacgag aaggacctgg
     1381 agaccgatgg ggacggcggc tggctgaccg actaccccgc gccgccggcc acgctgctcc
     1441 ccggcgaggt gggcatctac ggaccgctcg acaccgggac agccgacctc ctgatcgtca
     1501 gctacggcaa cggcctgcgc ctgtcgctgc aggccgcgcg gcggctgcgc gaggagcacg
     1561 agatcgccgc gcgggtgctc gacatccgat ggctgaaccc gcttccgcac caggcgatcc
     1621 gcgagcaggc ggcgaacatc ggcgccgtgc tggtggtcga cgagtgccgt cgcaccggtg
     1681 gcggcgtggc cgacgcaatc ctggcggacc tcgcggaggc cggcatcggt cgtcgcctgt
     1741 ggtcggtccg ggcggtggac agcttcgtgc cgctcggaac tgccaccagc gcggtgctga
     1801 tcgggacgga ccagatcgtg gccggggcgc tcaccacggt gagcggcagg ctgcacgcat
     1861 gatgcagcgt gcggtcctcg cctgccctgg gcgcgggtcg tacgccgcgg cctcgcttgg
     1921 ctcgctccag cctgatcacc cgtgggtgct ccgcgccgag cagctgcgcg ccggctacgg
     1981 ccttgagcca ctgctggacc tcgatcgggc ggaccgcttc gacccgagcc gtcacctcca
     2041 gccgatccac gcctcgccat tgatcttcct ggtctcgatg ctcgatgccg aggctgccgt
     2101 cgccgaccac caggtcacag cggtcatcgg aaactcgctc ggctggtaca cggcgctcgc
     2161 cgcagccggg gcgcttccct tcgacgacgc cttccggctg gtgcaggaga tggccatcct
     2221 gcagcaggag ccactgccag ccggaggtcc cggtgggcag gtgatctacc cactggcgga
     2281 cgccgcctgg cgacccgacc cgcaactgca ggcggcggtg gcggccgccc tggccgaacc
     2341 gagcgtcaac ggcgacggcc acgtgcacga gagcatcgac ctcggtgcgt atgccgtgct
     2401 ggccggcgac gaggccggcg tcgagcgcct gctcggccgc ctcgccccgg tgcgtgttgg
     2461 cgagcggctc ttccccctgc gcctctccca gcatggcccg taccacaccc cactggtcgc
     2521 gcacgtcgcc gcggcagcgc ttgaccagct cggcgacctg gagtggcgag cgccctcggc
     2581 gaccatcatc gacgggcgtg gcgttcgctg gacgccgtgg tccacggacc ccgccgcgct
     2641 gcgcgactac accctcggcg agcaggtgac gacgccgttc cgattcgcgg tcagcgtgcg
     2701 cgtcgccctg cgcgaggagg cgcccgacgt gctggtgctg cccggaccgg gcaactcgct
     2761 ggggggcatc tgcgggcaac tcgtggtggc tgagggctac cgtgggatcc gttcacgcga
     2821 ggacttcgag acggtccagc gctcggagac gccgatcgtg ctgagcatgc accgctgacc
     2881 gatggccgag cccgatgcca gcgggttcgt ctcgctcccc gcgccgatca acctggccct
     2941 cacgcttggc cccatcgcac atggtcgcgg cgacccgacg atccgcttcg accctgacgg
     3001 catctggcgc gccacgcgca cgccggaagg ccccgccacc ctgcgcctcc gcgccgcgaa
     3061 gggtggcgtt caggtctcgg cgtggggcgc cggggccgcg aatgcggtcg gaggagcctc
     3121 cgacctggtc ggggcgaacg acgatcccgc cgtcctcgtt gccgagcatc ggctcattgg
     3181 tgagctggcg catcgctttc ccggtctgcg gttgccgcgc accaaccggc cgttcgaggc
     3241 gttgctgccc gcgatctgcg agcagaaggt ggccggcgtc gaggcacgag cggcgttccg
     3301 cgggatcatc gccagccacg gcgagcccgc acccggtccc gccggccttc gcctcccgcc
     3361 gagccccgcg acactcgcgg cgctccccta tttcgccttt catcgcttcg gcctggagcg
     3421 gcgccgcgcc gacctgatca ggcacgccgc gcagctggct gcgcgtatcg aggccgcgcc
     3481 ggtggcggag gcctacgccc ggctcgcagc ggtgccgggg atcggtccat ggacgctggc
     3541 cgaggtgggc cgcatcgcct tcggagatcc cgatgcagtc agcgtcggcg actaccacct
     3601 gcccaatgtc gtggcctggg cgttggcacg cgagccacgc gcggacgacg ccaggatgct
     3661 cgagctgctg gagccgtacc gcgggcagcg gggccgcgtt cagcgcctcc tcgaggcgag
     3721 cgggatccgg gctccgcgcc atgggccgcg catggctccg cagcgcatcg ccgagatctg
     3781 aggaacaaag gtggcgtccc cggcaggatt cgaacctgcg acccgctgct tagaaggcag
     3841 ccgctctggt ccactgagct acggggacaa gccggtccta ggatagccaa cgccaccgcc
     3901 ggggccggcc attggtagac tgccggcgac atgccagacg accgcatcat cgccccggtg
     3961 cagctgcagc cgacccagat cgagaccgat cagcagctac tgcgcgacga ggatctgctc
     4021 aacgaggagc acctccgccg gatggaccag gtgctgctgc ggcgcaacct cgaccacgca
     4081 cgcatgttca gccgcaagct gtcactcgag ctccagcagt tcgtccatct ctacacgccc
     4141 gaggccaacg agctgcggcg gcgcatcgag aagtacatga tcatggaacg cctgatcgaa
     4201 cgcgagatgg cgcggcgcca cagctactag acgcggcatt tgtggacatc cgcacccagt
     4261 ggatggcgcg atgtggacaa ctcggtgcgc cctggcaaca tcgggcgatt cggcgatcag
     4321 gagacggccg ccgcgtcacc tcgacgtgac ggcggggcga cccaaagagc cgaacggtca
     4381 gcccggcatc aggggaacgg cgacgccctc gatcggctcc gctggcaccg cgaggacggc
     4441 ccgcacccaa ccggagagcc tggacatctc gaacaggctg aaggtcagtc ccctggccgc
     4501 gaactggtcc tgatcccagc cgttgaactc gcggcgagcg gcgacggcgc acatttcgtc
     4561 atacagctcg ggccagccga caggcttgcg gtcgtggcag aagcggatga attccgcgac
     4621 atcgggtggc ggcggatcgg cgggaggatc tcccggcatc ggtgccgcgg acgacggcat
     4681 gagccgctgc aacatggtgg gcgtgcctcc gattggcgag aggacggatg gttcccagac
     4741 gggaccgatg attgcgaccg acctgaggat aggcgtcggc gccgaatggt acgggcctca
     4801 accggccttg cgaagggggt ctgtccacca agttatccac attcgtttcc acacgaaggg
     4861 gcttgggtgg acatgcggcg gttcgacggt ggaaaggtct gaccgcgggc gcgtggtagg
     4921 ttggcggcat ggagatcggc gtggtggtgc cgcagggctg gacgggcgaa tacgacggct
     4981 gggaagcgcg gcgcgcgtgg gagcggacgc tggcggtcac gcgccaggcg gagcgtctcg
     5041 gcttcgaatc ggtctgggtc ttcgatcact tccagaccga gcccgagccg accgatgagc
     5101 tgaccttcga gtccttcacg acgctcgccg cactggccgc ctcgaccgag cgcgtgcggc
     5161 tgggccatgt cgtgatctgc accgcgtatc gcaacccggc actgacggcc aagatgatcg
     5221 gcaccctcga cgtgatcagc ggcggccgga tggacctggg gatcggcgcc ggctggaagg
     5281 aagacgagtg gcgggcgtat ggctacggct tccccgacac gaagacgcgc ctggccaccc
     5341 tcgccgacca cctcgaggtg atcacccgca tgctgggacc cggccgagcg acgtttgccg
     5401 gcaggtacgc atcggtcacc gatgccatca acgtcccacg cggcctccag gagccgcgcg
     5461 tgcccgtcat ggtcggcggc aacggcccga aggtgacctg gcgcctggcc gcccgcttcg
     5521 ccgatgagct taacctcgac tggctgaccc ccaacgaggt gggcgaggcc aagccggtca
     5581 tcgcctctcg atgcgaggag atcgggcggg acccctcgac cctgcggctg tcggtgaaca
     5641 tcagccgata cgtcctcaag gacgccggac cctcgcgcgt cgagctgctg gcgggctatc
     5701 gcgaggtcgg cgtgcatcgc gtgatggggc tcatccagga gacggccgac agcgacgagc
     5761 cgctcgaatc cctggccgcc gatgccaccg cagccggctg cgagttgcac caatccgcgt
     5821 gaacgccatg gttcgagggt cactcagagc gagtggccga ggcccgcacc tcctggacgg
     5881 gcggcgcttc cgggcgcgaa ctgggtgtcg aaactgaagc attcatcgca tcggccagca
     5941 gcttctggaa cggaagagcc cccagtcgct cgaacacctg ggcgacctcc tctcctacag
     6001 cgcgggccgc aggttccgag ggccccagca tgatgaggag gttgagtccg cataaggccg
     6061 cctcaaagtc caaaccgagc tcccgccacc gacggatcgc ctcggcgaag cccgcgagcg
     6121 cctcgggccg cccctcgagt gccgccagcg ccgcttcggc ctcgcgcccc atggcagacg
     6181 ccacgcgccc ggtctggttt ccgattagag cgagcgcatc ggtgagggcc tggcggtcgc
     6241 cgatccaggc ggcggcccgg ccggcggtct gcagcgacgc gctatcaggg gcgatgctca
     6301 tctggtacga gcgtcgcgcg aggtcgagcg ccgcccggtg gtcgcccaac ccgagcgcga
     6361 caagagcatg cacctcgtcg actgaggcct gcgcctggac ctcggtcatg tccgccattc
     6421 ggtcggcgag ccattggaac tcatcgttca cgtccacgcc ccgcaggcca cggatctcgg
     6481 cgagacgcat cctggaggcg gtgtcggttt ccaactggac ggcctcttcc aactcgggca
     6541 gtagccagtc ccagtcgccg atgcggatcg ccagctcggc cgcgttgctg agcaaataga
     6601 aggcgtagcc gcgcatgccg aggtggcgcg tcagctccac gccctcgcgc gctacccggt
     6661 aggcgagctc aggatcctcg gcggcggccg cgtagctgag attcactcgc gcgcgcaggc
     6721 ccacgtcagc caagccgtag gtcgtgctcg cctccaccgc tccgacgagg gtcacgatgg
     6781 cctcgcgcag ccgtcccatg ccggccagcg cgggcccgcg cgtgacgaga agctcgatgg
     6841 tctcgcgggt cagctccagg cgctccgcgg ttgggagggt tccatccacg accttgaccg
     6901 cgtcctctcc gcgtccgagg agcagatagg ctcgcgccag ctctgcggcg agccggacgt
     6961 actctcgcgt ctctgcgagg tcgcggtact cgtcaaacgc cggttccagg attgccacac
     7021 attcggcgat tcgcccgtgg gcactgaggg caagcgagtt cccggtgatg gcctccatca
     7081 atccgcggcg gtcccctgcc acctgcgcca actgtagcca gcgggccatg tgcgccatcg
     7141 gctcatcaat gagccccgcg tagaggccag cattgccggc cgccgcatgc agctgcgctt
     7201 cctccacagg atcggtcgtc acctcgagcg cctgctccag aaagcgtgtc gcctgaagga
     7261 aagagcccag cgaccgggca cgttcggccg ctccacgcag cgccacgcgc gcctgggccg
     7321 cgaccgcgtc gccttccggc ccctccggct gcgccttgta cgccgccagg tagtgctccg
     7381 ccagcgcccc ggcgattccc tcgtcgtcga gcgcctcgaa atagcgcgcc gcagcgatgt
     7441 gcagccgccg ccgatcacgc ttggccagcg tcccataggc cacctcacgg atgaggccct
     7501 gcacgaagag gtattggccc tgttcggggg atcgcgggtc ggcctccagc gacagcatct
     7561 cgcggcgtac gagcgcgcgc aggcgcgtca cgacctcatc cgtcggcagt ccgctgacgg
     7621 ccgccagggc atcgatgctg aacgtcttgc cgatcaccga tgccgcctgg agcagcgtcc
     7681 gatcggccgg gtcgaggcca tcaaggcggg aggcgatgag ggcatgcagc gaggcgggca
     7741 cggacagctc cgagaggtcc cccgtcggcc gatagatccc gtcctggagc gtgacgaggc
     7801 catcgcttag cagcatgcga acggtctcga cggcatagag cggaatgccc tccgcccgtt
     7861 caaggatccg ctcaaccacc gacgagggca gccccggaac cagctcggca agcagctcgc
     7921 gcatcagctc gggctcgagc ggctccagca ccagcgaggt gaagttgcgc cggccggcac
     7981 cccagtcgct gcgccggtcg aggagctcgg gtcgcgccag gctgatgagg tagatcggcc
     8041 ggtcgcgcga ccactcgagg atgtggtcga tgaagtcgag caggccgtca tcggcccatt
     8101 ggaggtcttc aaagacgagg acgacgacac cgcgctcggc cacacgctcg aagaagaccc
     8161 gccaggcggc gaagagcgac tcgcgatcgg gacgctcatc ggcctcggtc gcctcgagac
     8221 caagcagctg gagcatgcgg ggctccaccc accggcgctc ggtttcatcg gtcacccatt
     8281 catcgagcat gccggtcacc tttgaacgcg ccgcagccgg atcctccgac tcgttgattc
     8341 ctgcgcggcc acggaccatc tcggccagcg cccagaagct gattccttcg ccgtaggccg
     8401 gtgaacggcc ctggtg
//
LOCUS       DATCQR010000015         5045 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_186790, whole genome shotgun sequence.
ACCESSION   DATCQR010000015 DATCQR010000000
VERSION     DATCQR010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5045
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186790"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..888
                     /locus_tag="VIN32_00525"
     CDS             <1..888
                     /locus_tag="VIN32_00525"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEY8921096.1"
                     /translation="AGEQDMRLMGGLRAKMLWTYLTMVVGSLALAGIPIFAGFFSKDE
                     ILGEAFGRGYYVFWAVGLLVAFMTAFYTFRMVYMTFHGRWRGPADVWQHVHESAPTMV
                     APLVILAVPTILIGLLLGLPPEGGVIHGWLEEVFRGAEDAGAGILPGSLAAGGHAGFS
                     LFGLGGLLLLLGAGVGAAGIWLAYRWYVRDPEAPARFVARIPLGLGPGMYAASVNKYY
                     VDDLYSLVWARGGVLLGNALWWFDAKVIDGAVNGAGWLAQRYGGLLRRTQTGHVQNYA
                     LGMAAGLVVVLVAFLVFRA"
     gene            885..2408
                     /locus_tag="VIN32_00530"
     CDS             885..2408
                     /locus_tag="VIN32_00530"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015724558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEY8921097.1"
                     /translation="MTDLPVLSLVVFTPLIGVALILLVPGHNHRLIRWIALLAALASL
                     AFSLNLLGYKPDGAEFQFREDLSWISAFGMRYTLGVDGLSVVLVLLTTVLSVVSIFYS
                     WDPIQTRVKEYYATMLLLMIGMLGVFVSLDLFLFYVFWELSLIPMYLVIGIWGGPRRI
                     YATVKFVIYTLVGSLLMLVAILAVAIAYASAGHAFTFSYETLRGFGYTDSLQALAFVA
                     FFLAFAIKVPMWPFHTWLPDAHVEAPTAGSIILAGVLLKLGGYGFLRYSLPLAPDAAV
                     AFAPIVIGLAVVAVLYGAMVSMVQPDLKKLIAYSSVSHMGFVMLGVFVFNTQGLQGAV
                     FQMISHGVTTGALFLLVGVIYERTHDRQIAHMGGLNARLPRYAAIFGLFTFASIGLPG
                     LSGFIGEFLVVLGSFQYSGWVAAATMLVVIISAVYMLWMFQRVFFAVPSDWMRRAWPT
                     LTDMSRNEWLALTPLILLVVALGVLPGPVLDAISAPVQRILEAVNGSHGLTSLTLPW"
     gene            2419..3873
                     /locus_tag="VIN32_00535"
     CDS             2419..3873
                     /locus_tag="VIN32_00535"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HEY8921098.1"
                     /translation="MNPDELRTILPEIALTALVLVVLTTDLFLRREDKWLLTPITVIG
                     LGFVGYACWTVWGVNQTVFAGFYRVDDLSVFFKVATVVIGVLSALFAPSYLAQRRLPL
                     GEFNVILLFSLTGMFVLASASDLITLFIGLELMVMPAYLLAGFHKTDRYSNEGGLKYF
                     LLGSFASAILLFGISWTYGLTHSTHLSEIGGLLSGGGPAGVIAIAMLTVGATFKAAAV
                     PFHYWTPDAYQGAPTPITGFLSVGPKLGAFALLVRLFAEGLGASGADWYGVVAVLAVL
                     TMTGGNVVALAQTNVKRMLAYSSIAHTGYILAGLAAFAHATDPAIRTEGIDAILFYVL
                     GYGVMNIAAFSVVGMLQRDPRRFGGLAAFAGLAGRAPALAAAMAVLLLSLTGIPPTVG
                     FFAKLYVLVATVDAGVAWLAVAIALNAALAAFYYLRVIVYMYMREPEADPAPIDRSPL
                     TAFALAISVAGVLLLGLFPDPIISFLRASAAGLL"
     gene            3901..4581
                     /locus_tag="VIN32_00540"
     CDS             3901..4581
                     /locus_tag="VIN32_00540"
                     /inference="COORDINATES: protein motif:HMM:NF016119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HEY8921099.1"
                     /translation="MKGSTAPGIVLPLVLAVAGASLMLAGQLPVDKPGAILPGIPDAS
                     EVAVATPSPSPESSGFTPVPTPTGTPIPDDWVAVQLEVSSVDLNVAVKEALPGQCGFP
                     PDDGAYVCHGSQPGRGANAYIIGHALQHLFKRLWNVMLGAEVRVLMSDGVVLRYVVTE
                     IHPNQACPDSRANPMPNPPLALRYAKPGCPGARWANPTDHERLTLQTSQGYNRNWGEL
                     IVIAEPLF"
     gene            complement(4578..>5045)
                     /locus_tag="VIN32_00545"
     CDS             complement(4578..>5045)
                     /locus_tag="VIN32_00545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921100.1"
                     /translation="ATGWGVATELGRLQRDLVIFGAGFGLTVSPRATAAVEAAGASAY
                     GVASAMLQVTRTIGMSVGLALLTSIGQNRIDELTALINDPSRRDGLVTALGHPEFVGV
                     DPRSSLALVDLLEAWSRGEAAGVLRLVFAIAVAVAAVTILPALLVAGGRRASG"
BASE COUNT          644 a         1822 c         1665 g          914 t
ORIGIN      
        1 gccggtgagc aggacatgcg ccttatgggc ggcctgcggg cgaagatgct gtggacctac
       61 ctcaccatgg tggtcggctc gctggcgctg gccgggatcc ccatcttcgc gggcttcttc
      121 agcaaggacg agatcctggg cgaggccttc ggacgcggct actacgtctt ctgggcagtg
      181 ggcctgctgg tcgccttcat gacggccttc tacaccttcc ggatggtgta catgaccttc
      241 catggccgat ggcgtggccc ggccgacgtc tggcagcacg tccacgagtc ggcgccgacg
      301 atggtcgcgc cgctcgtcat cctggccgtc ccgacgatcc ttatcggcct gctgctgggc
      361 ctgccgccgg agggcggcgt gatccatggc tggctggagg aggtgttccg cggcgccgag
      421 gacgcgggag ccgggatcct gcccggctcg ctcgccgccg gcggccacgc cggattcagc
      481 ctcttcgggc tcggcgggct gctcctgctc ctcggggcag gcgttggagc agcaggcatc
      541 tggctggcgt accgctggta tgtccgcgat ccggaggccc cggcgcgctt cgtggctcgc
      601 atcccgctgg ggctcggccc ggggatgtat gccgccagcg tgaacaagta ctacgtcgat
      661 gacctctaca gcctggtctg ggcgcgcggc ggcgtgctgc tcggcaacgc gctgtggtgg
      721 ttcgacgcca aggtcatcga cggagccgtg aatggggctg ggtggttggc tcagcgctac
      781 ggcggcctcc tgcggcgcac gcagaccggc cacgtccaga actacgccct gggaatggcc
      841 gccgggctgg tggtcgtgct ggtcgccttc ctggtcttcc gcgcatgacc gatcttcccg
      901 tcctgagcct ggtcgtcttc acgccgctga tcggcgtggc gctgatcctg ctggtgcccg
      961 gccacaacca ccgactcatc cgctggatcg ccttgctggc cgcgctcgcc agccttgcct
     1021 tcagcctcaa cctgctgggt tacaagcccg atggcgccga gttccagttc cgcgaggacc
     1081 tctcgtggat ctccgccttc gggatgcggt acacgctcgg cgtggacggc ctgtcggtcg
     1141 tgctggtgct gctgacgacg gttctgagcg tggtcagcat cttctactcc tgggatccga
     1201 tccagacccg ggtcaaggag tactacgcca ccatgctgct gctgatgatc gggatgctgg
     1261 gcgtcttcgt cagcctggac ctgttcctct tctatgtctt ctgggagctc agcctgatcc
     1321 cgatgtacct ggtgatcggg atctggggcg gcccgcggcg catctacgcg accgtcaagt
     1381 tcgtgatcta cacgctggtc gggtcgctgc tgatgctggt cgccatcctg gccgtggcga
     1441 tcgcctacgc atcggccgga cacgcgttca ccttcagcta cgagacgctc cgcggcttcg
     1501 gatataccga ttcgctccag gccctcgcct tcgtcgcctt cttcctggcc ttcgccatca
     1561 aggtcccgat gtggccgttc cacacctggc tccccgatgc gcacgtcgaa gcgccgaccg
     1621 ccggcagcat catcctggcg ggggtgctcc tgaagctggg cggctatggc ttcctgcgtt
     1681 attcgctgcc gctggcaccc gacgcggcgg tcgccttcgc cccgatcgtc atcggcctgg
     1741 cggtggtcgc cgtcctgtac ggcgccatgg tcagcatggt gcagcccgac ctgaagaagc
     1801 tgatcgccta ctcctcggtc agccacatgg gcttcgtcat gctcggcgtc ttcgtgttca
     1861 acacccaggg gctgcagggc gccgtcttcc agatgatcag ccacggggtc accaccggcg
     1921 ccctcttcct gctggtcggg gtcatctacg agcgcaccca cgaccgccag atcgcgcaca
     1981 tgggcgggct gaacgcccgg ctgccgcgct acgcggcgat cttcgggctg ttcaccttcg
     2041 ccagcatcgg gctgcccggg ctatcgggct tcatcggcga gttcctggtg gtgctcggct
     2101 cgttccagta ctccggctgg gtcgcggccg ccacgatgct ggtcgtgatc atcagcgcgg
     2161 tctacatgct gtggatgttc cagcgcgtct tcttcgccgt cccatccgac tggatgcgcc
     2221 gcgcctggcc gaccctcacc gacatgagcc gtaacgagtg gctggcgctg accccgctga
     2281 tcctgctcgt ggtggcgctc ggcgtgctgc ccggccctgt gctggacgcg atctcggcgc
     2341 cggtgcagcg catcctcgag gcggtcaacg gctcgcacgg cctcaccagc ctgacccttc
     2401 cgtggtgacc gcgctcccat gaaccccgac gagctgcgca cgatcctgcc ggagatcgcg
     2461 ctcaccgccc tggtgctggt ggtgctcacc accgacctct tcctgcgtcg cgaggacaag
     2521 tggctgctga cgccgatcac cgtcatcggg ctcggcttcg tggggtatgc gtgctggacg
     2581 gtgtggggcg tcaaccagac cgtcttcgcc ggcttctacc gggtcgacga cctgtcggtc
     2641 ttcttcaagg tcgccaccgt ggtgatcggc gtgctgtccg cgctctttgc cccgtcctat
     2701 ctggcccagc gccgtctgcc gctcggcgag ttcaacgtga tcctgctctt cagcctgacc
     2761 ggcatgttcg tgctcgcctc ggcgagcgac ctgatcacgc tcttcatcgg cctggagctg
     2821 atggtcatgc cggcctacct gcttgccggc ttccacaaga cggatcgcta cagcaacgag
     2881 ggtgggctga agtacttcct gctcggcagc ttcgccagcg cgatcctcct cttcgggatc
     2941 agctggacct acgggctgac gcattcgacc catctctcgg agatcggcgg cctgctgagc
     3001 ggcggcggac cggccggcgt catcgcgatc gcgatgctca ccgtcggcgc caccttcaag
     3061 gcggccgcag tgccgttcca ctactggacc cccgacgcgt accagggggc accgacaccc
     3121 atcaccggct tcctgtcggt ggggccgaag ctgggcgcct tcgcgctgct ggtccgcctc
     3181 ttcgccgagg ggctgggggc gagcggcgct gattggtacg gcgtcgtcgc ggtcctggcg
     3241 gtcctgacca tgaccggcgg caacgtcgtg gccctggccc agaccaacgt gaagcggatg
     3301 ctggcctact cgagcatcgc ccacaccggc tacatcctgg cggggctggc cgccttcgcc
     3361 cacgccaccg atcccgccat ccgcaccgag gggatcgacg cgatcctctt ctacgtgctc
     3421 ggctacgggg tgatgaacat cgccgccttc agcgtggtgg ggatgctgca gcgcgacccg
     3481 cggcgcttcg gcggcctggc cgcctttgcc ggcctcgccg gacgcgcccc cgccctcgcg
     3541 gcggcgatgg cggtcctgct cctgtcgctg accggcatcc cgccgacggt cggcttcttc
     3601 gccaagctct acgtgctcgt cgccaccgtc gacgcggggg tcgcgtggct ggcggtggcg
     3661 atcgccctga acgcggcgct ggccgccttc tactacctcc gcgtgatcgt ctacatgtac
     3721 atgcgcgagc cggaggcgga cccggcgccg atcgaccgct caccgctcac cgccttcgcg
     3781 ctcgccatct cggtggccgg cgtgctgctc ctcggcctct tcccggatcc gatcatcagc
     3841 ttcctgcgag catcggcggc cggactgctc tgagcgggcg ctcacggtag actcggcccg
     3901 atgaagggct ccaccgcgcc ggggatcgtg ctcccgctcg tcctcgcggt ggctggtgcc
     3961 tcgctcatgc tcgccgggca gctgccggtc gacaagcccg gggccatcct gccggggatc
     4021 cccgatgcca gcgaggtcgc ggtcgccact cccagcccga gccccgagtc ctccgggttc
     4081 acgccggtgc cgacgccgac cggcactccc atccccgacg actgggtcgc ggtccagctc
     4141 gaggtctcct cggtcgacct gaacgtcgcc gtcaaggagg cgctgccggg ccagtgcggc
     4201 ttcccgccag acgacggggc gtacgtctgc catggctcgc agccgggccg tggggcgaac
     4261 gcgtacatca tcggccacgc gctccagcac ctgttcaagc gcctctggaa cgtgatgctg
     4321 ggagcggagg tcagggtcct gatgtcggac ggcgtggtgc tgcgctacgt cgtgaccgag
     4381 atccacccca accaggcctg ccccgactcg cgcgccaacc cgatgcccaa cccaccgctg
     4441 gcgctgcggt acgcgaagcc gggctgcccg ggcgcgcgct gggccaaccc gacagaccac
     4501 gagcggctca cgctgcagac gagccagggc tacaaccgca actggggcga gctgatcgtc
     4561 atcgccgagc cgctcttcta gcctgaggcg cggcgccctc ccgcgaccag cagcgcgggg
     4621 aggatcgtga ccgcggccac cgccaccgcg atcgcgaaga cgagccgcag caccccggcc
     4681 gcctcgcctc gcgaccacgc ttcgagcagg tcgacgagcg ccaggctcga gcgtgggtcg
     4741 acgcccacga actccgggtg ccccagcgcg gtcaccagcc catcgcggcg cgacgggtcg
     4801 ttgatgagcg cggtcagctc gtcgatccgg ttctggccga tgctggtcag cagcgccagg
     4861 ccaacgctca tcccgatcgt gcgggtcacc tgcagcatgg cgctcgccac gccataggcc
     4921 gatgcgccgg ccgcctccac ggcagcggtg gcccgcggcg agacggtcag gccgaagccg
     4981 gccccgaaga tgaccaggtc ccgctgcagc cggccgagct cggtggcgac cccccagcct
     5041 gtggc
//
LOCUS       DATCQR010000016         2289 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_140460, whole genome shotgun sequence.
ACCESSION   DATCQR010000016 DATCQR010000000
VERSION     DATCQR010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140460"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..936
                     /locus_tag="VIN32_00550"
     CDS             <1..936
                     /locus_tag="VIN32_00550"
                     /inference="COORDINATES: protein motif:HMM:NF019844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEY8921101.1"
                     /translation="TSVWVPWLASTVAKSATFIGTPGHSYDFRVIAVDYRGNSQPLVS
                     SKSAGGPTLAVGGFGRVAVDSVNVRSGAGTGFTSLDQLSTGATVAVLGGPIASGGYQW
                     YQVQFDFTEWPSADYPRIGWVAAGTGTTAYVMPTKPPNMITVAASAKPARLMFGLGTH
                     TGYRFGSMGALTSVKSYTLAKGSSAPVDTRASITNQSGTWFHVTSGVWAGYWIRQSSV
                     SWLSTSPVAATSSTYTTFNPAAGLTFKRGTHTGYRFSTVGAMTAQKTYTLSKDSGAPT
                     TVRRTLSNQSGTWFYVSGGVWAGYWVRASDVVYLP"
     gene            965..1699
                     /gene="rph"
                     /locus_tag="VIN32_00555"
     CDS             965..1699
                     /gene="rph"
                     /locus_tag="VIN32_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013580836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEY8921102.1"
                     /translation="MSTIAQTRRPDGRAANQLRPVKIVHDYLKFAEGSVLIRVGDTRV
                     ICAATIEDRVPGWMRGKGGGWITAEYSMLPRAGTERSQREASAGRIGGRTHEIQRLIG
                     RSLRSCIDLPRLGERTITLDCDVVQADGGTRTASITGAYVALARALNKFGMSHLLVGK
                     VAAVSVGIVGGQTLLDLNYAEDSTAEVDFNLVMTDDDGLIEVQGTAERRSFSRDQMNQ
                     MLDLGSAGIRQLFEVQRAAIEAAAAE"
     gene            1696..>2289
                     /locus_tag="VIN32_00560"
     CDS             1696..>2289
                     /locus_tag="VIN32_00560"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009146 - purine nucleoside triphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HEY8921103.1"
                     /translation="MSELLLATANPGKQREFSRLLQSLEGRLVLPQQIGLSLDVPEPY
                     VTYAENAAAKAAAYALASGLPALADDSGLEVAALEWGPGPRSARYGGPEVDDSAALIL
                     ELLGDATDRRARMVCWLALAIPGAAPDGTVRSPRIELFSGVVEGAIATERRGAGGFGY
                     DPIFMLPSGITTAELPEGEKDLISHRGQAVAAALPRLR"
BASE COUNT          381 a          778 c          772 g          358 t
ORIGIN      
        1 acctccgtgt gggtcccctg gctggcctcg acggtggcca agagcgccac gttcatagga
       61 acgccagggc actcatatga cttccgcgtc atcgcggtcg actaccgggg caacagccag
      121 cccttggtct cgtcgaagag cgccggcggc ccgaccctgg ccgtgggcgg attcggccga
      181 gtcgcggtcg acagcgtcaa cgtgagatca ggcgccggca ccggcttcac gtcgctcgac
      241 cagctctcca cgggagccac ggtggctgtc cttgggggcc cgatcgcttc cggcggctac
      301 cagtggtacc aggtgcagtt cgacttcacc gaatggccct ctgccgacta tccacgcatc
      361 ggatgggtgg ccgccggcac cggcaccact gcctatgtca tgccaaccaa gccacccaac
      421 atgatcaccg tcgcggcgag cgccaagccg gcgcggctca tgttcggtct tggtacgcac
      481 accgggtacc ggttcggaag catgggcgcc ttaacatcgg tgaagagcta cacgctggcg
      541 aagggctcga gcgcgccggt cgacacacgc gcgtcgatca ccaaccagtc cggcacctgg
      601 ttccacgtca ccagcggcgt gtgggccggt tactggatcc gacaatcaag cgtctcatgg
      661 ctgtccacgt cgccggtggc cgccaccagt agcacgtata ccaccttcaa cccagcggca
      721 gggctcacgt tcaagagggg cacccacact ggataccggt tcagcaccgt gggtgcgatg
      781 acggcccaga agacctacac cttgtcgaag gattctggcg ctcccacgac cgtgcgccgg
      841 acgctctcca accagtccgg cacgtggttc tacgtcagcg gcggcgtctg ggccggctac
      901 tgggttcgcg cgtcagacgt ggtgtacctg ccctgaccgc ggccgccgcg tagaatcggc
      961 ggcgatgtca acgatcgccc agactcgacg ccccgacggc cgcgccgcca accagctccg
     1021 gccggtcaag atcgtccacg actacctgaa gttcgccgag ggatcggtcc tgatccgcgt
     1081 cggcgacacc cgggtgatct gtgccgcgac gatcgaggat cgggtcccgg gctggatgcg
     1141 aggcaagggc ggtggctgga tcaccgccga gtactcgatg ctgccgcgcg cgggcacaga
     1201 gcgcagccag cgcgaggcga gcgcggggcg gatcgggggc cggacgcacg agatccagcg
     1261 tctgatcggt cgatcgctgc ggtcgtgcat cgacctgcca cgccttggtg agcggacgat
     1321 cacgcttgac tgcgacgtgg tccaggccga tggcgggacc cgcacggcca gcattacggg
     1381 tgcgtacgtg gccctcgccc gggcgctcaa caagttcggg atgtcgcacc tgctggtcgg
     1441 gaaggtggcc gccgtcagcg tcggcatcgt cggtgggcag acgctactgg acctgaacta
     1501 cgccgaggac tcgaccgcgg aggtcgactt caacctggtc atgaccgacg acgacggcct
     1561 gatcgaggtg cagggaactg ctgagcgacg atccttctcg cgcgaccaga tgaaccagat
     1621 gctcgacctc gggtcggccg gtatccgtca gctgttcgag gtccagcgcg cagcgatcga
     1681 ggctgccgcg gccgagtgag cgaactgctg ctggcgaccg ccaatccggg caagcagcga
     1741 gagttcagcc gtcttctgca gagcctcgaa ggccggctgg tgctcccaca gcagatcggg
     1801 ctcagccttg acgtcccgga gccatacgtc acctacgccg agaacgccgc cgccaaggcc
     1861 gccgcctatg ccctggcatc cggactcccc gccctggccg atgattcggg gctcgaggtg
     1921 gccgccctcg agtggggccc cgggcctcga tcggcgcgct atggaggtcc ggaagttgac
     1981 gatagcgcag cactgatcct ggagctgctc ggcgacgcga ctgaccgacg ggcgcgaatg
     2041 gtctgctggc tcgccctggc aatccccggc gctgccccgg acgggaccgt gcgctcacca
     2101 cggatcgagc tgttcagtgg ggtcgtggaa ggggcgatcg caaccgagcg gcgtggcgca
     2161 ggaggattcg gctatgaccc gatcttcatg ctgcccagcg gcatcaccac cgccgagctg
     2221 cccgagggcg aaaaggacct gatctcgcat cgtggtcagg cggtcgctgc cgcgctgccg
     2281 cgcctgcgg
//
LOCUS       DATCQR010000017        10430 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_1203, whole genome shotgun sequence.
ACCESSION   DATCQR010000017 DATCQR010000000
VERSION     DATCQR010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 10430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10430
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1203"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="VIN32_00565"
     CDS             <1..196
                     /locus_tag="VIN32_00565"
                     /inference="COORDINATES: protein motif:HMM:NF042641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6582 domain-containing protein"
                     /protein_id="HEY8921104.1"
                     /translation="DEDGTGHLPINDETHVRNAIARWNQTDFPSKTAKEQARRKILAA
                     ARRHKIDVSDGDRVAKPAST"
     gene            201..1424
                     /locus_tag="VIN32_00570"
     CDS             201..1424
                     /locus_tag="VIN32_00570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8921105.1"
                     /translation="MGRITADEVLMAAAPRTTTTSRPTRGKDAGGSEDTGRAVLPGVE
                     FHAFLDTFGVAVYTTDAEGGITYFNDDAVTLWGRRPELGEQWCGSWRLYWPDGQPMAH
                     DECPMAIALKEDRPIRGVETELERLDGTRAVFMAYPTPLHDTSRHLVGAVNVLVDVTE
                     RRKAEEALRAAAEALAASNQVKDEFLGLVSHELRTPVTTIFGNAHLLRGRAGLLATEE
                     ADSMLTDIADESNRLLGIIENLLLLTRLGSGTPPDQEPQLIAHVARNAVDGYRRHHPE
                     RSIAFVVEPGQMVVEADRGHLEMLLDNLLTNATKYSPASTEIEVRVSSDKQEACVAVL
                     DRGIGIGDADPSQLFTAFYRTEAAKMRTAGLGIGLAVCQRIVDSLGGRLWASPREGGG
                     TEAGFALPLAVVSPD"
     gene            1429..2166
                     /locus_tag="VIN32_00575"
     CDS             1429..2166
                     /locus_tag="VIN32_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013704432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HEY8921106.1"
                     /translation="MPSDLRGREGVMGGRSMIRDRTAAALVAEAFGTFLFFFVGAGSV
                     VLGDYLLANGGTSPGLVGVALAHGLALAVLASALGAVSGAHFNPAVTLGVWIMGRISA
                     TGAALYVVAQLVGGLAAGLALKVAFADSWQASKIGTPALGAGITPVLGIGIEAVLTAL
                     LLLVVIGTTVDARAPKLGGLAVGLAVTADILVGGPLTGAAMNPARWFGPAVAAGAYAD
                     WYVWWIGPAIGAAVAALIYRTALAEAS"
     gene            complement(2226..2609)
                     /gene="rplL"
                     /locus_tag="VIN32_00580"
     CDS             complement(2226..2609)
                     /gene="rplL"
                     /locus_tag="VIN32_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019414589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEY8921107.1"
                     /translation="MSKDQILETIDGMTVLELSELVKAFEERYGVTAAAPAAAAAPAS
                     AGDGGGAPAAAEEQTEFDAVLTEVGPNKILVIKAVRELTGLGLKEAKDLVDAAPKAVK
                     EGVAREEADAVKEKLAEAGASVDVR"
     gene            complement(2648..3190)
                     /gene="rplJ"
                     /locus_tag="VIN32_00585"
     CDS             complement(2648..3190)
                     /gene="rplJ"
                     /locus_tag="VIN32_00585"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEY8921108.1"
                     /translation="MPTDAKRQAIAELTEMLRSSSALAVADYRGLKVSEMQSVRRSLR
                     ANGVELHVAKNRLLKIAAAEAGLSELTSLLAGPTAVATSSGDETALAKALQEALRPYS
                     KVVALRGGLLGGVAVDAAVFQQLASLPSRDVLLSRMAGAMASPIAGMAGVLAANLRNL
                     VGVLSAVADQKRASEGGSAA"
     gene            complement(3390..4097)
                     /gene="rplA"
                     /locus_tag="VIN32_00590"
     CDS             complement(3390..4097)
                     /gene="rplA"
                     /locus_tag="VIN32_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014096512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEY8921109.1"
                     /translation="MMPHGKKYIDAAKLVEQDRRYLIGEACELLPRVSISKFDATMEL
                     HLRLGIDPRHADQLVRGTVVLPHGTGRTTRVIVFAQGEKAQDALRAGADEVGGDDLVK
                     KIDDGWFDFDVAIATPDMMGTVGRLGKKLGPRGLMPNPKAGTVTFDIERAVSEIKSGR
                     IEFKVDRAGIVHAPVGKASFTPDQLSANIATLVDAVQRAKPSGAKGTYMRTLTVAPTM
                     GPGLRIDIPSALAAASA"
     gene            complement(4094..4519)
                     /gene="rplK"
                     /locus_tag="VIN32_00595"
     CDS             complement(4094..4519)
                     /gene="rplK"
                     /locus_tag="VIN32_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007391508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEY8921110.1"
                     /translation="MAKKIRAILKIQAPAGRATAAAPVGPALGQHGINIMEFIKAFNE
                     RTASQSGYVVPAEITVFEDRSFTFVTKAPLAAALLKRAAGVESGSAEPNRTKVGKVSQ
                     RQLREIAETKMADLNANDVEHAMRIVAGTARSMGIEVTD"
     gene            complement(4523..5167)
                     /gene="nusG"
                     /locus_tag="VIN32_00600"
     CDS             complement(4523..5167)
                     /gene="nusG"
                     /locus_tag="VIN32_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEY8921111.1"
                     /translation="MKDEQATEQATETGARNEARQAARLSREAPQPDDPYAAERTTKQ
                     WYVIHTYSGYENKVKANLEHRIESMGVEDQIFQVLVPMEEEIEIKNGQRQTVNKKVFP
                     GYVLVEMVMTDESWYVVRNTPGVTSFVGSGNRPTPLMEGEVKKILKQMGVEAPKFKLQ
                     FTKGQSVRVKDGPFAEFIGTVDEVNPERNKVKVLVSIFGRETPVELDFLQIDKL"
     gene            complement(5167..5349)
                     /gene="secE"
                     /locus_tag="VIN32_00605"
     CDS             complement(5167..5349)
                     /gene="secE"
                     /locus_tag="VIN32_00605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEY8921112.1"
                     /translation="MFRGIRQYLTEAWSELKKVAWPTRQTVINLTLIVVAVSIAVGAY
                     IAVLDTAFHALIDQVL"
     gene            complement(5401..5477)
                     /locus_tag="VIN32_00610"
     tRNA            complement(5401..5477)
                     /locus_tag="VIN32_00610"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5441..5443),aa:Trp,seq:cca)
     gene            complement(5493..5660)
                     /gene="rpmG"
                     /locus_tag="VIN32_00615"
     CDS             complement(5493..5660)
                     /gene="rpmG"
                     /locus_tag="VIN32_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010936698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEY8921113.1"
                     /translation="MVAKTENRPVISLACTTCKERTYSTRKNKRNDPGRMELNKFCPR
                     CRQHTLHRETK"
     gene            complement(5993..7036)
                     /locus_tag="VIN32_00620"
     CDS             complement(5993..7036)
                     /locus_tag="VIN32_00620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921114.1"
                     /translation="MNIALPFLSSVLSFIFAAMVGDQWLRRRQPYQLVWTIGLLWYGI
                     SAGTEFLGSAFGWSEPLYRTWYLIGAFGVASYLGLGTVFLLNRTRFGYFVAFSLFAGG
                     LFSILAAAARAKEGVPASDGAVLTVVAFSTVGAVALVIATRFDRRLVAPLSAGILAVA
                     SGIVLFLVVFAPLPAPGYALDPTTGVPVGTAFPSDLRILTPPFNITGAFALVFGAIYS
                     AYIFMPKRRLLRIEGGPPVIGAIGRAVAVVVNFFASIPGAIRSSRAGTLNSRVPATLL
                     IALGGFVPGLTSGLNRFGITWSFFLGELLGIVFIFVGFLVSIEVFSDIRLPFTRVVLV
                     RRKQAEPPGAAPA"
     gene            complement(7090..7165)
                     /locus_tag="VIN32_00625"
     tRNA            complement(7090..7165)
                     /locus_tag="VIN32_00625"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7130..7132),aa:Thr,seq:ggt)
     gene            complement(7256..7340)
                     /locus_tag="VIN32_00630"
     tRNA            complement(7256..7340)
                     /locus_tag="VIN32_00630"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7304..7306),aa:Tyr,seq:gta)
     gene            7531..7977
                     /locus_tag="VIN32_00635"
     CDS             7531..7977
                     /locus_tag="VIN32_00635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921115.1"
                     /translation="MHRWNPRTRLLRGGLIAIVLSVAGSLVAYASLPSAADEGQARAA
                     AGAADGQAGQSQADTHPAENTGEGQSTALDQLAENQDRLLATLGDVLARLSANENVPA
                     AATDAVQRVIDRLGGDIGLNRAMDAVGGNTGAPDLPEAATNHPGRP"
     gene            8020..8628
                     /locus_tag="VIN32_00640"
     CDS             8020..8628
                     /locus_tag="VIN32_00640"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY8921116.1"
                     /translation="MDPPEQQPNVEALVERARAGDQAAIGAIYDRLAGRVYRFALFRV
                     GSRADAEDLMQRIFLKMIESLPRYQPRGVPFEAWFFRLARNTVIDHLRARRRLQPLET
                     IAEVDSTRPGPEESAELAMEFRRLETALEDLTPVQQEVIGYRFMAGLSAREIGLIIGK
                     REGSVRALQFRALQALRRSLTRDSLGADMAEARILPESELES"
     gene            8625..9296
                     /locus_tag="VIN32_00645"
     CDS             8625..9296
                     /locus_tag="VIN32_00645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921117.1"
                     /translation="MSRPGPRETGDIELMLDRYAAERLAPHKDELDRARAAMFAGWTG
                     AGSLALLPHRSLRRGWSLAGAAAVLVIAGAGLVAAESGPGQPLYGLRLAMGNVTLPHE
                     EPAHQRGLADQLDDRLSEVGAAARTGDLLGAQAAIDEYLHTLRELSRDGVTDPAILAL
                     LQRHQDTLQDLLGVTPAQAAGGVQQAIEAAGKVNGVVPPIESALPHPTPPHAADQSPR
                     ATGKP"
     gene            complement(9316..9570)
                     /locus_tag="VIN32_00650"
     CDS             complement(9316..9570)
                     /locus_tag="VIN32_00650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921118.1"
                     /translation="MPELADVLGLSALVAGSLEYAILIRHRQERRLSLSFVDDADVAA
                     REALTRVNLRDSRWPMRRWTADGTRFRIESAWPRWPSRRG"
     gene            complement(9644..9718)
                     /locus_tag="VIN32_00655"
     tRNA            complement(9644..9718)
                     /locus_tag="VIN32_00655"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9684..9686),aa:Thr,seq:tgt)
     gene            complement(9709..>10430)
                     /gene="rlmB"
                     /locus_tag="VIN32_00660"
     CDS             complement(9709..>10430)
                     /gene="rlmB"
                     /locus_tag="VIN32_00660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEY8921119.1"
                     /translation="GRAASRLLVAHSARSGPELNEVLAAARAASVPVETVDANRLRQL
                     AGFDGHQGVLLEVGERRFADLAEVLAAAAKREHDPLLLILEHLQDPTNFGTLLRSAEA
                     AGVDGAIFPERGAAPLSAAAVKASAGASEHLLLARVDGLGETTHELQERGIRLIAADQ
                     EAPTAAWDADLTGPIAIVVGSEGSGVSGAMRRRCDLLVRFPMAGRIASLNAATAGALL
                     LFEVVRQRAAGARLESGPTPT"
BASE COUNT         1696 a         3538 c         3462 g         1734 t
ORIGIN      
        1 cgatgaggat ggcaccgggc acctccccat caacgacgag acccacgttc gcaatgccat
       61 cgcccgctgg aaccagaccg atttcccgtc gaagacggcg aaggagcagg ctcgacggaa
      121 gatcctggcg gccgcgcgcc gccacaagat cgatgtctcg gatggcgaca gggtcgcaaa
      181 gccggcctcg acctgagata gtgggacgaa tcaccgctga tgaggtgctg atggcggcgg
      241 cgccacgcac gaccacgacg agcaggccca cccgcggcaa ggatgcaggt ggttccgagg
      301 acactgggcg tgcagtccta cccggcgttg agttccacgc cttcctcgat accttcggag
      361 ttgccgtcta cacgaccgac gccgaaggcg ggatcaccta cttcaacgac gatgccgtca
      421 cgctgtgggg tcgccgtcct gagcttggcg agcaatggtg tggctcatgg cgcctgtact
      481 ggcccgatgg gcagccgatg gcccacgatg aatgcccgat ggctatcgcg ctgaaggagg
      541 acaggccgat ccgaggggtc gagaccgagc tcgagcgcct cgacggcacg cgcgccgtct
      601 tcatggccta cccgaccccc ttgcatgaca cctcccggca tctcgtcggc gccgtcaacg
      661 tgctggtcga cgtgaccgag cgtcgaaagg cggaggaggc gctgcgcgcg gctgctgagg
      721 cgctcgccgc ctccaaccag gtcaaggacg agttcctggg cctggtgtcg cacgagctcc
      781 gaactccggt caccacgatc ttcggcaatg cgcacctcct caggggccgg gccgggctgc
      841 tcgcgaccga ggaggcggac tcgatgctga cggacatcgc cgatgagtcg aaccgcctct
      901 tgggcatcat cgagaatctc ctgctcctta cgcgcctcgg gtccgggacc ccgcctgacc
      961 aggagccgca actgatcgcg cacgtggcgc gcaacgcggt cgatggctac cgcagacatc
     1021 acccggagcg ctccatcgca ttcgtggtcg agcccggtca gatggtggtc gaggccgatc
     1081 ggggccacct tgagatgctc ctcgacaatc tcctgaccaa cgccacgaag tacagtcccg
     1141 cgtccactga gatcgaggtg agggtctcct ccgataagca ggaagcatgc gtcgcagtcc
     1201 tcgatcgcgg aatcggcatc ggcgatgcgg accccagcca gttgttcacc gccttttatc
     1261 ggaccgaagc ggccaagatg cgtaccgccg gcctcggcat cggcctggcg gtctgccagc
     1321 ggatcgtcga ctcgctgggt ggtcgactct gggccagccc gcgggaaggt ggcggcacag
     1381 aagccggatt cgcgctaccc ctggcggtgg tctcgcccga ctgacagaat gccctccgac
     1441 cttcgcgggc gcgaaggcgt gatgggaggc aggtcgatga tccgggatcg cacggcggcg
     1501 gcactggtgg ccgaggcgtt cggcacgttc ctcttcttct tcgtgggcgc aggctccgtg
     1561 gtgctgggcg actacctcct cgccaacggc ggaaccagcc cgggcctcgt tggcgtcgcg
     1621 ctggcgcacg ggttggcact tgcagtcctc gcctcggcgc tgggcgcagt ctccggtgcc
     1681 cacttcaacc cagcggtcac gctcggggtc tggatcatgg gccggatcag cgcgaccggg
     1741 gcggccctct acgtcgtcgc acagctggtc ggcgggcttg ccgcgggcct tgcgctgaag
     1801 gtggcattcg cggattcctg gcaggcatcg aagatcggca ccccggcgct cggagccggc
     1861 ataacgccgg tcctcgggat cggcatcgag gcggtgctca cggcgctgct gctgctggtt
     1921 gtcatcggga caaccgtcga tgcgcgcgcg cccaagctcg gtggcctggc cgtcgggctg
     1981 gcggtaacgg ccgacatcct ggtcggcgga ccgctgacgg gcgccgccat gaatccggcg
     2041 cgctggtttg gaccggccgt cgcggcaggc gcctacgcag actggtacgt gtggtggatc
     2101 ggcccggcca tcggtgcggc cgtcgcggcg ctgatctacc gcactgccct ggccgaggcg
     2161 agctaggggt aagcgatagg gccggcccgc aaccgggtcc ggccctcagt gagaacgctg
     2221 gggccctagc ggacgtcgac cgaggcgccg gcctcggcga gcttctcctt gaccgcgtcg
     2281 gcctcctccc gggcgactcc ctccttgacc gccttcggcg cggcatcaac caggtccttg
     2341 gcctccttga gaccaaggcc ggtcagctcg cgcaccgcct tgatgaccag gatcttgttc
     2401 gggccgacct cggtcagcac cgcgtcgaat tccgtctgct cctcggcggc agccggtgcg
     2461 ccaccgccgt cgccggccga ggctggtgct gcggccgccg ccggggcggc agcggtcacg
     2521 ccgtagcgct cctcgaatgc cttgaccagc tcggagagct cgaggacggt catgccgtcg
     2581 atcgtctcga ggatctggtc cttgctcatc gtcttggtag ccatgctcgt gtctctttcc
     2641 ttcctcgtca ggcggcgctg ccgccttcgc ttgctcgctt ctggtcggca actgcactga
     2701 gcacgccgac gagatttcga agatttgctg ccaggacccc ggccatcccc gcgatcggag
     2761 aggccatggc tccggccatg cggctgagca gcacgtcgcg gctcggcagg ctcgccaact
     2821 gctggaacac cgccgcgtcg acggcgactc cgcccagcag gccaccccgc agggcaacca
     2881 ccttggaata cggtcggagg gcctcctgca acgccttcgc cagcgccgtc tcgtcgccag
     2941 agctcgtcgc gacggcggtc ggaccggcga gcaaggaggt cagctcggag aggcccgcct
     3001 cggcggcggc gatcttcagc aggcgattct tggcgacgtg cagctccacg ccgttggcgc
     3061 gcaacgacct ccgcaccgac tgcatctctg agactttcag gccccggtaa tcggccacgg
     3121 ccagggcgga gctggagcgg agcatctccg tcagctcggc gatcgcctgg cgtttcgcat
     3181 cggtcggcat gatgttgtcc taacgaacct ccgtgacatc aaaaaagtcc tcgcgcacgc
     3241 atcggcacga ggaccgtctc cggtcgtctc attgcctcgg cgtgccgccg gctctcttcg
     3301 gccggcgatt ggcccggggc ttgcacccgg tgcacaacgc tgtctgtggc ttctcttcgg
     3361 ctgtcccgat gagtcgggtc gctgacctcc taggcgcttg ccgccgccag cgccgacggg
     3421 atgtcgattc gcaacccggg gcccatggta ggggcaaccg tcagtgtgcg catgtaggtg
     3481 cccttggcgc cgctcggctt cgcccgctgc acggcgtcca cgagcgtcgc gatgttggcc
     3541 gacagctggt cgggcgtgaa gcttgccttg ccgaccggag cgtggacgat gccggcccgg
     3601 tcgaccttga actcgatgcg gcccgacttg atctcgctga ccgcccgctc gatgtcgaag
     3661 gtgacggtgc ccgccttggg gttcggcatc aggccacggg gcccgagctt ctttcccagg
     3721 cgtccgacgg ttcccatcat gtcgggcgtg gcgatcgcga cgtcgaagtc gaaccacccg
     3781 tcgtcgatct tcttgacgag gtcgtcgccg cccacctcat cggctcccgc acgcagggca
     3841 tcctgtgcct tctcgccctg ggcgaagacg atcacgcgcg tggtgcgacc ggtgccgtgt
     3901 ggaaggacga ctgtcccgcg aaccagctgg tccgcatgac gcgggtcgat ccccagccgc
     3961 aggtgcagtt ccatagtggc gtcgaacttc gagatggaga cgcgcggaag cagctcgcag
     4021 gcctcgccga tcaggtagcg acggtcctgc tcgaccagct tcgcagcgtc gatgtacttc
     4081 ttgccgtgcg gcatcagtcg gtcacctcga tgcccatgct gcgcgccgtg cccgccacga
     4141 tccgcatggc gtgttcgacg tcatttgcat tcaggtcggc catcttcgtc tccgcgatct
     4201 cgcgcagctg ccgctgcgac accttgccga ccttggtgcg gttcggctcg gcagagccgc
     4261 tctcaactcc agcggcgcgc ttcaggagcg cggcggccag cggggccttg gtgacgaagg
     4321 tgaaggatcg gtcctcgaag acggtgatct cggccggcac cacgtagcca gactggctgg
     4381 cggtgcgctc gttgaaggcc ttgatgaact ccatgatgtt gatcccgtgc tggccgagcg
     4441 ccggcccgac cggagccgcc gctgtggcgc ggccggcagg tgcctggatc ttgaggatgg
     4501 cgcgaatctt cttggccatg ggctacagct tgtcgatctg gaggaagtcg agctcgaccg
     4561 gcgtctcgcg accgaagatg ctgaccagca ccttgacctt gttgcgctcc gggttgacct
     4621 cgtcgacggt gccgatgaac tcggcgaacg gcccatcctt gacgcgcacg ctctggccct
     4681 tcgtgaactg gagcttgaac ttgggcgcct cgacccccat ctgcttgagg atcttcttga
     4741 cctcgccctc catcaacggg gtgggcctgt tgccggatcc cacgaagctg gtcacgccgg
     4801 gggtgttgcg gacgacgtac caggactcat cggtcatgac catctcgacc agcacgtagc
     4861 ccgggaagac cttcttgttg acagtctgcc gctggccgtt cttgatctcg atctcctcct
     4921 ccatcggcac cagcacctgg aagatctgat cctccacgcc catggactcg atgcggtgct
     4981 cgaggttggc cttgaccttg ttctcgtagc cggagtaggt atggatcacg taccactgct
     5041 tggtggtccg ctccgcggca tacggatcgt ccggctgcgg cgcctcacgg gaaaggcgag
     5101 ccgcctggcg cgcctcgttg cgcgccccgg tctccgtcgc ctgctcggtg gcctgctcgt
     5161 ccttcatcac agcacctggt cgatcagcgc atggaaggcg gtgtcgagca ccgcgatgta
     5221 ggccccgacc gcgatcgaca cggcgaccac gatgagggtc aggttgatga ccgtctggcg
     5281 cgttggccag gcgaccttct tcagctcgga ccaggcctcg gtgaggtact ggcgaatgcc
     5341 gcggaacacg atggcttcag ctcgctcctg ctgtgatggt tcttgattga ccgatgaagc
     5401 tggcaggggc gacaggactt gaacctgcaa ccgctggttt tggagaccag tgctctacca
     5461 aattgagcta cacccctacc gacgcgctct gcctacttcg tctcccgatg cagcgtgtgc
     5521 tgccggcatc gcgggcagaa cttgttgagc tccatccggc cggggtcgtt tcgcttgttc
     5581 ttgcgggtgc tgtacgtgcg ctccttgcag gtcgtgcacg ccagcgagat gacaggtcga
     5641 ttttcggtct tggccacgat gcgatgtcct cgtcagtcgc ccgtcgccgc ccacgccaaa
     5701 caagggctgg tcaccggggg aaagcgccgt gattctaggc tggctccgag tcagatgtca
     5761 acgcgggcac gctgggccag cgcgctgggg tcggccacaa ctgaccgcca ggttgtcact
     5821 tccgacctgt gaagggtcgt cgttgatcac taggcgaggc atcggtgccg tcggttcgtc
     5881 tttctgacta gttggagggt cgggctggca cgcgagtggt cgaactgctc acccggcggt
     5941 cgtgtagagc cggcttcagg cccagttcgc ggaggaagcc gccgcgcgct gatcaggccg
     6001 gcgcggcacc cggcggctcg gcctgcttgc gtcgcaccag gacgaccctg gtgaacggca
     6061 gccggatgtc gctgaagacc tcgatgctga ccaggaagcc gacgaagatg aacacgatcc
     6121 ccagcagctc gcccaggaag aagctccagg tgatgccgaa gcggttgagg ccgctggtca
     6181 ggcccgggac gaaaccgccg agcgcgatca gcagcgtcgc aggcacgcgc gagttcagcg
     6241 tccctgctcg ggaggagcga atggcgcccg ggatcgaagc gaagaagttg accaccaccg
     6301 caactgcccg cccgatcgct ccgatcacgg gcggaccgcc ctcgatccgc aagaggcgcc
     6361 gcttcggcat gaagatgtac gccgagtaga tcgctccgaa cacgagcgcg aacgcgccgg
     6421 tgatgttgaa gggcggcgtc aggatccgca ggtcgctcgg gaatgcagtc ccaaccggca
     6481 ctcccgtcgt cgggtcgagc gcgtatcccg gagcaggcag cggggcgaac accaccagga
     6541 agaggacgat ccccgaggcg acagccagga tgccggctga caggggtgcc accagccgcc
     6601 ggtcgaagcg ggttgcgatc acaagcgcca ccgctcccac ggtcgagaag gccaccacgg
     6661 tcaggacggc gccgtccgat gcggggaccc cctccttcgc gcgggcggcg gcggccagga
     6721 tgctgaacag gccgccggcg aagaggctga aggccacgaa gtatccaaat cgggtgcggt
     6781 tgagcagaaa gacggtgccc agccccaggt acgaggccac accgaatgcg ccgatcaggt
     6841 accaggtacg gtacagcggc tcgctccagc cgaaggcgct gcccaggaac tcggttccgg
     6901 cactgatccc gtaccagagc aggccgatcg tccacaccag ctggtatggc tggcggcgac
     6961 gcagccactg gtccccgacc atcgcggcga agatgaatga caggaccgat gagaggaacg
     7021 gtagggcaat gttcaccgcg gatccgacct cctacgcgac atctgatgcg aatggccacc
     7081 cgcattgggt ggagcccacg tccgggctcg aaccggagac ctcgtcctta ccaaggacgt
     7141 gctctgccaa ctgagctacg tgggcgcgcg cagattctgc aggacgggcg agatgatagg
     7201 caaccatatg cctatcttgg caaatctcgg catcggatcc ttcccgaacg gacgatggtg
     7261 ggcagggagg gaatcgaacc cccacagtcg aagacggctg atctacagtc agcggagctc
     7321 accacctgct caacctaccc acgccttcga tcggcaggtg gtgcgctgcc gaccgagcgg
     7381 gagtatagcg aaggaccgat cggcgcctca accgtgcgtc cggagatgtc gctgcataac
     7441 gaattgggcc acgcgccgtt cctacgagta ccaatccccg cgagcggcaa ccgccgctac
     7501 gcgcaaatca cgcacgagga atgacttctc atgcaccgat ggaacccacg aacccgactg
     7561 ctgcgcggtg gcctgatcgc catcgtcctc tcggtcgcag gctctctagt cgcctatgca
     7621 tcccttccct ccgctgctga cgaggggcag gcacgggccg ccgcgggtgc ggccgatggt
     7681 caagccgggc agagccaggc ggatacgcat ccggcggaga acaccgggga ggggcagtcc
     7741 actgccctcg accagctggc cgagaaccag gatcgcctcc ttgccacact cggggatgtc
     7801 ctcgctcggc tgagcgccaa cgagaacgtc cctgccgccg cgaccgacgc ggtccagcgt
     7861 gtcatcgaca ggctcggtgg agacatcggc ctcaaccgcg ccatggatgc agttggcggc
     7921 aacaccggtg ccccggacct gccggaggcc gcaacgaacc accctggccg gccctgacgt
     7981 cgagatcctc catgccgcgg caggtacgct tgccgcggca tggacccacc tgagcaacag
     8041 ccgaatgtcg aagcgctcgt ggaacgagcg agggcagggg accaggcagc cattggcgcc
     8101 atctacgatc gcctggccgg ccgcgtctat cgcttcgcgc tcttccgtgt cggcagccgt
     8161 gccgatgcgg aagacctcat gcaacggatc ttcctcaaga tgatcgagtc gctcccccgg
     8221 tatcagcctc gaggagttcc cttcgaggcg tggttcttcc gcctcgcgcg caacaccgtg
     8281 atcgatcacc tccgcgctcg acggcgcctc caaccgctcg agaccatcgc agaggtcgac
     8341 agcacccggc ccgggccgga ggagtccgcc gagttggcca tggagtttcg ccgcctcgag
     8401 accgcccttg aggacctgac cccggtgcag caggaggtca tcggctaccg cttcatggcc
     8461 ggcctcagtg cgcgcgagat cggcctgatc atcggaaagc gcgaaggcag cgtgcgagcg
     8521 ctccagttcc gcgcgctcca ggccctgcgt cgatcgctca cccgtgactc gcttggggca
     8581 gatatggcgg aggcgcgcat cctgcctgag tcggagctcg aatcgtgagc cggcctggac
     8641 ctcgggagac gggcgacatc gagctgatgc tggatcgtta tgccgccgag cgtcttgcgc
     8701 cgcacaagga tgagctcgac agagcacgcg ccgcaatgtt cgccggatgg acgggcgccg
     8761 gatccctggc tcttctgccg caccgttctc tccgtcgcgg ctggtcgctc gcaggggctg
     8821 cggcggtgct ggtgatcgcc ggtgccggcc tggtggcggc tgagagcggc cccgggcagc
     8881 ctctgtacgg tctgcgcctg gcgatgggga acgtgacgct gcctcatgaa gaacctgctc
     8941 atcagcgcgg gttggcggat caactggacg accggcttag cgaagttgga gcggctgccc
     9001 gcacgggcga cctcctgggt gcgcaggcgg ccatcgacga gtacctgcac acgctcaggg
     9061 agttgtcgcg tgatggcgtt accgacccgg cgattctcgc tctactccaa cgccaccagg
     9121 acacgctcca ggaccttctc ggggtgacac cggcccaggc ggccggtggc gttcagcagg
     9181 cgatcgaggc cgcgggcaag gtcaacggtg tcgtgcctcc aatcgagagc gccctccccc
     9241 atccaacgcc accgcatgct gccgaccaat cgccccgtgc cacggggaag ccgtagctaa
     9301 ccggcgttcg gacggctatc cgcgcctcga cggccagcgc ggccaggcac tctcgattcg
     9361 gaagcgggtg ccatccgccg tccagcgacg catcggccac cggctgtcgc gcaggttcac
     9421 ccgagtcaga gcctcgcgag cggccacgtc ggcgtcgtcc acgaacgaga ggctcaagcg
     9481 ccgctcctgc cgatgtcgga tcagaatcgc gtattcgagg cttccggcca caagcgctga
     9541 aaggccgagg acgtctgcga gctcaggcat gtgcggaaca ttgcggcgct cggagggggc
     9601 gtgcctagtc gggaaatggt cctgtccgcc atgggaccgg acatggagcc gacggaggga
     9661 gtcgaaccca caaccggcgg tttacaaaac cgctgctcta ccgttgagct acgtcggcgt
     9721 cgggcccgat tctagtcgag cccctgccgc tcgctgacga accacctcaa agagcaggag
     9781 cgcgcccgcc gtggcggcat tgaggctcgc gatcctgccg gccatcggga agcggaccag
     9841 caggtcgcag cgtcgtcgca tggcgcccga tacgccgctc ccctcgctgc cgaccacgat
     9901 cgcgatcggt ccggtcagat cagcgtccca cgctgcggtc ggcgcctcct ggtcagcagc
     9961 gatcagccgg atgccacgct cctggagctc gtgcgtcgtc tcgcccagtc catcgacgcg
    10021 cgccagcagc agatgctcgc tggcaccggc cgaggccttg accgctgcgg ccgacagggg
    10081 tgcggctccc cgctccggga agatcgcgcc gtccacgccg gccgcttccg cggatcgaag
    10141 cagggtgccg aagttggtcg ggtcctgcag gtgctccagg atgagcagta gcgggtcatg
    10201 ctcacgcttc gcagctgcag ccaggacttc ggcgaggtcg gcaaagcgac gctcccccac
    10261 ctcgagcagc acgccctggt gcccgtcgaa gccagcgagc tgccgcaggc gattcgcatc
    10321 caccgtctcc accggcaccg atgccgcgcg cgcagcggcc aggacctcgt tcagctccgg
    10381 gcccgaccga gcgctgtgag cgacgaggag gcgggaggcc gccctgccgg
//
LOCUS       DATCQR010000018        14640 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_125116, whole genome shotgun sequence.
ACCESSION   DATCQR010000018 DATCQR010000000
VERSION     DATCQR010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 14640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14640
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125116"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="VIN32_00665"
     CDS             complement(<1..302)
                     /locus_tag="VIN32_00665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921120.1"
                     /translation="MERIFLEAIDACSDQAAERVGNSDPIELLRRPPPALLFIPLDGA
                     DIDEHPHEFLYEERIPIGLPKDQGTQIGRELRRAEQARGHTGAVVLAQRFERHLHEP"
     gene            351..2084
                     /locus_tag="VIN32_00670"
     CDS             351..2084
                     /locus_tag="VIN32_00670"
                     /inference="COORDINATES: protein motif:HMM:NF024579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921121.1"
                     /translation="MHALLPDQIATDRALGDLYTAGFIRERRRVPELEHVFKHALVHD
                     AAYESILLQQRRQLHRLVAMTIEREYADRVDDFFSVLAFQFANAEEWGKAWEYLIKAG
                     DQAVRIAADAEALALYREAFAAHERAFGEGWEPEERGVLEGKMGEALLRRGVHFEARD
                     HLERSLTYLGRPFPRGRSGVRVAVLGHALRQAGHRLTPIFFRAKPAAHIEEVIRTSET
                     MSWIDFFADPERFVLTALRALNYSEEAGYVFGTAYGSTAIGFVCSALPQHRMAGWYFR
                     RALPIAEHSGHPLAIGLAYTGLGYHAQHALGDHGAASAYYEKATSAYRQSGDLWRWSP
                     PASLWSQLLRYLGETDRAVRMAEEIVRAGEEGGDAVMRMTGLLRHGCALMQAGDLDRA
                     ESALRATIELGERIPDYQHLVCAQGFLGAALLHRARVNEATRLLEDAAALAARHRVRT
                     FYATQARISLAEAYLSTAEAGDAAAMGKARSAVKRCMSQGRIDIEARPPAFRVKGTLC
                     WLNGRREEARIAWDRSLAWARDTGGRYDEGLTLLEIGRRTGDRHAIERAGTVFEEIGA
                     RARLQQALAVV"
     gene            2089..3246
                     /locus_tag="VIN32_00675"
     CDS             2089..3246
                     /locus_tag="VIN32_00675"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY8921122.1"
                     /translation="MFPFTSTPQQLEFRDAVRAFASREIAPIAIQHDRTGESPLGVHH
                     RFWREGLAEPLLPRPDGEHSPYLVMFCIAAEELAYACAAIASLIMLPLFFNRLVLQLL
                     AEPARSEFRAQLLSNPTITSFAATERASGSNLLLMDTRAERVDGGYVLTGRKQFSTNL
                     RHASKAIVVARTGGSGERATSAMTWFLVPTTHPGVAIGDRWETLGLRAIDVSPMELHD
                     ARIPESWRLGDEGRGIWLMNEALPQSRTGIGAIAVGIARRARDEVLAYGSRRRLYGDK
                     LYKLQDYRFRIAEMEKDIAAARALTYLSALKHDQGLSNNKEASIAKLNAGQVVMRVTE
                     AASVMLGGIGYTQESVVEKLFRDARHTAIVEGTDPTHKELIFADLIRKGGY"
     gene            3251..4084
                     /locus_tag="VIN32_00680"
     CDS             3251..4084
                     /locus_tag="VIN32_00680"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009979325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY8921123.1"
                     /translation="MGREDVARDAEAAFHYETSNDFFKLWLDPTMTYSCAYFATGSES
                     LEEAQIAKVDLAFERVKLQRGDRLLDIGCGWGAAAERAAERYGASAVGLTVSPEQFEL
                     ARRRERPGLALSYRLEGWETYREPCDVIVSFGAFEHFKVANYGAFFARCHELLPDGGS
                     MLIQTITIGKPSTSFKLFRFAHFLRKEIYPGAELPPPEVVVSHARLGGFELLHAESLR
                     PHYVRTLEEWLRNLKANRDAAIAATSEEMYAKNVRYLDGAAQYHRTGETNAYHFLFRS
                     V"
     gene            complement(4416..4601)
                     /locus_tag="VIN32_00685"
     CDS             complement(4416..4601)
                     /locus_tag="VIN32_00685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921124.1"
                     /translation="MNFETNRIMHRHGNDWVEMRPVDSHSPDRRDPERELIRGQRLFR
                     CSACDEEIQVTTGDPEG"
     gene            4799..5416
                     /locus_tag="VIN32_00690"
     CDS             4799..5416
                     /locus_tag="VIN32_00690"
                     /inference="COORDINATES: protein motif:HMM:NF013982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HEY8921125.1"
                     /translation="MRFSLIPRETKFFDMFVEDARNVLGAARLLEQFFRNYDDRERLA
                     SQLLDAEHAGDQMSHEIGHKLESTFVTPFDREDIHALISRLDDILDLIEEVADTCILY
                     NIDAPTPPAIQQAEIIVRQCEQLAQGLGKLRSFKGVEPFWIEVHRLENEGDRITRQAV
                     AELFKHDQDPIEIIKWKDVYALLEKAIDSAEDAANVIERIVVKHA"
     gene            5409..6410
                     /locus_tag="VIN32_00695"
     CDS             5409..6410
                     /locus_tag="VIN32_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007953756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HEY8921126.1"
                     /translation="MPEGFTAALLAVFGLAVLFDYINGFHDTANAIATSVSTRALSPR
                     LAIAMSATANFAGALTGTAVAKTIGAGLIAPQAEGGLVVAAALVGAIVWNLLTWRLGI
                     PSSSSHALIGGLLGASAAAVGFGAWQVDGILGKVILPLIGSPIVGFVGGFALMVVIFN
                     LFRRAHPKTMNDRFRRLQVLSAAFMAFSHGSNDAQKTMGVMTLALLSAGIIPEFKVPL
                     WVIILAASAISLGTAAGGWRIIRTMGSKVVKLDPVHGFAAETTAATIIFGASTLGMPV
                     STTHVISSAILGVGSSDRFRAVHWGVARRIGIAWVLTLPASGLVAALAYVLLRPMLG"
     gene            complement(6423..6782)
                     /locus_tag="VIN32_00700"
     CDS             complement(6423..6782)
                     /locus_tag="VIN32_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013013316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEY8921127.1"
                     /translation="MPVALMLLIGLAGGVLSGLFGIGGGVVIVPLLILILGFTGQQAA
                     GTSLAALLLPVGLFGAIQYWQAGQVNVLNAGLLAMGLLIGAFIGARLGLALPSEVIER
                     AFGVLLVVVGIRFVFFS"
     gene            6951..8144
                     /gene="pstS"
                     /locus_tag="VIN32_00705"
     CDS             6951..8144
                     /gene="pstS"
                     /locus_tag="VIN32_00705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEY8921128.1"
                     /translation="MTFRTQRRNALVVVLSSTLLIAACSSNNRPSSTPTSTAVASEGS
                     AGPSAGAVDLSQVPPKPAYSGAITADGATFPQPVYEQWTTDYATETGIQISYTGGGSG
                     QGVKDITANIVQFAGSDAPLKPQEQAAAEAANGPILHIPTVFGSIVLAYNVGGISKPL
                     NFSPDVIGKIFTGQIKNWNDPAIAADNAGVTLPNLAITVSHRNDGSGTTNAFTTWLCA
                     VSTDWKAKVDPCSGKEVTWPVGLGGKGNAGVAGNITGTPGAIGYVELAFAVQNSLTFG
                     NVKNKSGAFITPSLESTAAAATFDTIPDSLTFSVSDSSVADAYPIVTATWLLVYQQQD
                     KVNTNEQQAQAVVHFLIWTLDKGGASAQALGYAVLPDALRAAALDRIATVTYNGTPIV
                     DALYK"
     gene            8223..9209
                     /gene="pstC"
                     /locus_tag="VIN32_00710"
     CDS             8223..9209
                     /gene="pstC"
                     /locus_tag="VIN32_00710"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEY8921129.1"
                     /translation="MTADIAPVQPDSATALIRQRRLSLGDPLLGWAVRILSWSALAAI
                     ILMLLDTAGHSLKLLEHTSILDFLFGILWQPASTVYGALPFIYGTVVTSLLALFLAVP
                     LALGAALLVNEFLPLRVASPLAYSIELLAAVPSVVYGFWGVIVLVPALRPLEQAVAGS
                     PLGQIPLFSGPVPGPSYLAAGVILAIMIVPIVTSVARDAMRATPRLQREGALALGATH
                     WETIRHVVLPFARGGIFAGVILGLSRAIGETIAVLFVIGNQPQINASIFAPGYSLPSV
                     IAGEFAEANQPFHPEALIGLGVVLFLLSFTVNVAARVVVGRFEARAGGADVR"
     gene            9199..10068
                     /gene="pstA"
                     /locus_tag="VIN32_00715"
     CDS             9199..10068
                     /gene="pstA"
                     /locus_tag="VIN32_00715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEY8921130.1"
                     /translation="MFAEHSLHSISRSRRSRNRLVYLLCLVAGAIALLPLVLILFYLL
                     VEGLKAWSPAFLVSPPGGITSKSGGAMNAILGTLEMTGLGLAIATPMGLAVALYVAEY
                     REGRLAVIVRTAVDLVAGIPSIVIGLFVYSLVVITMGHFSGFAGAVALSLIMLPIIAR
                     TSEEMFKIVPRRLREGTMALAMPRWRAIVAVYIPTVAAGIVTGIVLAVSRAVGETAPL
                     LFTSLGSNFVVTDLNQPMSALPLYMFQNALNIGFPAARERAWAAALTLVAIVLIFNVL
                     GRWLAGRTQLKVR"
     gene            10186..10962
                     /gene="pstB"
                     /locus_tag="VIN32_00720"
     CDS             10186..10962
                     /gene="pstB"
                     /locus_tag="VIN32_00720"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HEY8921131.1"
                     /translation="MDAAFEVQHLNAYYGTKQAVRDVTFTAGKRAVTAIIGPSGSGKS
                     TVLRSLNRMLEVVPTARIDGQVLFQGIDIYAREIDPAAVRRTIGMVFQQPNPFPTMSI
                     YDNVAAAARFNGLVKGRAQTDELVERCLRQAALWDEVRDTLSKSGASLSGGQQQRLCI
                     ARALAVNPEAILMDEPCSALDPIATLQIEQLARELAQSYAIVIVTHNMQQAARVSDQT
                     IFMSMDAERAGYVVETGATSQIFTKPENRLTEDYITGRFG"
     gene            10977..11669
                     /gene="phoU"
                     /locus_tag="VIN32_00725"
     CDS             10977..11669
                     /gene="phoU"
                     /locus_tag="VIN32_00725"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEY8921132.1"
                     /translation="MEPASNADAARHSVRRGFDEALNATKDDVLRLGALVERALERSG
                     HALLERDVDLADQVRREDAQVNELQRSINTGITTLIATQAPVARDVRELLSLYHASAE
                     LERMGDYAVSIAKLTQQLASEPEIPVLPQIPKMELLCREQLRDAMRALVDVSEEQARA
                     VCARDDELDHLYRRVYEDSMNLMEAAPDKVRQATHMLFIAHHLERLGDRVTNIGEDVV
                     FLVTGQIEDLNP"
     gene            11686..12393
                     /locus_tag="VIN32_00730"
     CDS             11686..12393
                     /locus_tag="VIN32_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018085543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY8921133.1"
                     /translation="MKQRILVVEDDRTLRMALVFNLEREGYEVRSAVDGEQGLAAARD
                     GRLDLIVLDLMLPAMSGLEVLRTMRAEGIDTPVVILSAKGGEIDRVVGLKVGADDYIP
                     KPFSRPELLARVESVLRRQRRSEAAGDERLAEVRSGALAIDLSRHLATFSGEPLRLTT
                     KEFELLAHLAAHPGRIFTRDQLLTRIWPFDFGGDSRTVDVHISWLRSKLRALDGHNYF
                     RTVRGVGYAFAPRPDVH"
     gene            12383..13450
                     /locus_tag="VIN32_00735"
     CDS             12383..13450
                     /locus_tag="VIN32_00735"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8921134.1"
                     /translation="MSTDTLDAAAFAALLERTAGDILLVVDDQLRITRVGAGAAALAQ
                     RRVDELIGMSLIAAFGSAPLDALARHAARDRSPSLGEAELGPRGERAFAVDAVPLAGG
                     ELVLALHEITALRRAERVRRDFVTNISHELRTPLASVKLLAETLSAGAVEDAATAGQF
                     AIQIEREVDHLAQLVAELLDLSMIESGETALRLEPVDPSTVMPDVAERIGPVAARRGI
                     AVQLLPGPDGAEVRVNADAGRLGQALLNLAHNAVKYNRPGGEVRLGWEPSPGGVRFTV
                     ADDGIGISGAHQQRIFERFYKVDRSRAQESDDTTGGGSAGLGLAIVRHIAEAHGGSVG
                     VRSVEGEGSIFWVELPLAERA"
     gene            13470..13928
                     /locus_tag="VIN32_00740"
     CDS             13470..13928
                     /locus_tag="VIN32_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018684046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY8921135.1"
                     /translation="MSTQDTIPGPRDPRLETWRSFLVAHAQIRRQLERELQAEQSLGL
                     GEYEVLLLLARADARQLRMSDLADRLVLSRSGVTRLVDRLEAEALVERTSCTTDRRGS
                     WATLTDAGHRRLRRASPTHLRGVGAHFLDRIPPAELDALQRTLDRVLGEA"
     gene            complement(13925..>14640)
                     /locus_tag="VIN32_00745"
     CDS             complement(13925..>14640)
                     /locus_tag="VIN32_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921136.1"
                     /translation="AGSLDDASYERERSEAEARAVETLEPAPVEPPPRVVAPRRGRGS
                     IAALGALIALTMLVGFALPGSIGLAQRTVVNQALADAMSHEADRQAEIQRLLDLFATN
                     PRDAQVASDLADAYLAGDSQEDLQRAAVALQILLALQPENHSAYRRLINAYLVTAHYD
                     DAQATTDSYATIANPDEPDIPFYRGLIAFRRGDNATAVREFDRFLQLAPHDDRASMVS
                     SLRAEAAGALPGASRAPGG"
BASE COUNT         2418 a         4928 c         4845 g         2449 t
ORIGIN      
        1 ggctcgtgga ggtgccgctc gaaccgctgg gcgaggacga cagcgcccgt atggccgcga
       61 gcttgttcgg cacgacgcag ctcgcgtccg atttgcgtgc cctggtcctt gggaagaccg
      121 atgggaatcc gctcttcata gaggaactcg tgaggatgct catcgatgtc cgcgccgtcg
      181 agtggaatga acaggagcgc aggtggcggg cgacgaagga gctcgatcgg gtcgctattc
      241 ccgacacgct ccgcggcttg atccgagcac gcatcgatcg cctcgaggaa gatgcgctcc
      301 acgtgttaaa ggtagcctcc gtgatcgggc ggagctttgg agataggctc ttgcacgcgc
      361 tactcccgga ccagatcgcc accgatcggg ccctcggcga cctctatacc gccggcttca
      421 ttcgcgagcg ccggcgcgtc ccggagctcg agcacgtgtt caagcacgcg ctggtacacg
      481 acgccgcata cgagagcatc cttctgcagc aacgtcgtca acttcatcgc ctggtcgcca
      541 tgaccatcga gcgcgagtat gcggaccgcg tcgacgactt cttcagcgtg ctcgcatttc
      601 agtttgcgaa cgcagaggag tggggtaagg cttgggagta cctgatcaag gcgggcgacc
      661 aggcggtccg gatcgctgct gacgccgagg cgctcgcctt gtatcgcgag gctttcgcgg
      721 cgcacgaacg cgcgtttggc gaggggtggg aacccgaaga acgcggggtg ctggaaggga
      781 agatggggga ggcgttgctt cgccgcggcg tgcacttcga ggctcgtgac cacctggagc
      841 gctcgctcac ctacctaggt cgtcccttcc cgcgcggccg ttctggtgtt cgagttgcgg
      901 tgctcggcca cgcgctgcgg caggccggcc atcggctaac cccgatcttc ttccgagcca
      961 aacccgccgc gcacatcgaa gaggtcattc gaacgagcga gacgatgtcg tggatcgact
     1021 tcttcgccga tcccgaacgt ttcgtcttga cggcgctacg cgcgctgaac tactcggaag
     1081 aagcgggtta tgtcttcggg accgcctatg gcagcacggc gatcggcttc gtctgcagcg
     1141 cactgccgca gcacaggatg gcgggatggt acttccggcg ggcgctccca atcgctgagc
     1201 attccggtca cccgctcgcg atcggcctcg cgtacaccgg gctcggctac cacgcgcagc
     1261 acgccctggg cgaccacggc gcagccagcg cctactacga gaaggcgact tcggcgtacc
     1321 ggcaaagcgg cgatctctgg cgatggtcgc ctccggcgag cctctggtct cagctgctcc
     1381 gctacctggg cgagaccgac cgggccgtgc gcatggcgga ggagatcgtg cgcgcgggcg
     1441 aagagggcgg cgacgccgtc atgcggatga ccgggctcct ccggcatggc tgtgccctca
     1501 tgcaggcagg cgatctggat cgagccgaat cggcacttcg cgccaccatc gagcttgggg
     1561 agcgcattcc ggactaccag cacctggtgt gcgcacaggg gttcctcggc gcagcgctcc
     1621 tgcaccgcgc gcgggtgaac gaggcgacaa gactgctcga ggacgccgcg gcgctggccg
     1681 cgcgccaccg ggtgcgcacg ttctacgcga ctcaggctcg gatatcgctc gccgaggcct
     1741 acctgtcgac ggccgaggct ggcgacgccg ccgcgatggg caaagcccga tcagcagtga
     1801 agcgatgcat gagtcagggt cggatcgaca tcgaggccag gccgccggcg ttccgagtaa
     1861 agggcacgct gtgctggctg aacgggcgac gggaagaggc gcgcatcgca tgggaccgga
     1921 gtctggcgtg ggcgcgcgat accggcggac gctacgacga gggcctcacg ctcctcgaga
     1981 tcgggcgtcg gacaggtgac cgccacgcga tcgagcgtgc ggggaccgtg ttcgaggaga
     2041 tcggcgcacg cgcacgatta cagcaagcgc tcgcggtggt gtagcgcggt gttcccattc
     2101 acgtcgacgc cacagcagct cgagttccgc gacgccgtcc gtgcgttcgc gtcacgggag
     2161 atcgcgccga tcgcaattca gcacgaccgc accggagaat cgcccctcgg tgtgcaccac
     2221 cggttctggc gggaggggct cgcggagccc ttgcttccgc gtcccgacgg agaacactcg
     2281 ccgtacctcg tcatgttctg cattgccgca gaggagctcg cgtacgcctg cgcagcgatc
     2341 gcgagcctga tcatgctccc cctctttttc aaccgtctgg tcctgcagct gctcgcggag
     2401 cccgcgcgct ctgaattccg cgctcagctg ttgtcgaatc cgacgatcac ctcattcgct
     2461 gcgactgagc gtgcctcggg cagtaacctg ctcctaatgg acacgcgtgc cgagcgcgtg
     2521 gacggcgggt acgtcttgac tggtcggaag cagttcagta cgaacctccg tcacgccagc
     2581 aaggcgattg ttgtcgcgcg caccggcggg agcggagagc gagcgacatc tgcgatgacg
     2641 tggttcctgg tccccaccac acacccgggc gtggcgatcg gcgatcgctg ggagacgctc
     2701 ggcctccgcg cgatcgatgt atcgccgatg gagctgcacg acgcgcggat cccggagagc
     2761 tggcgactgg gtgacgaggg tcgcggaatc tggctgatga acgaggcgct gccccagtcg
     2821 aggacaggta tcggtgccat cgcggtaggg atcgctcgtc gcgcgcgcga cgaagtcctc
     2881 gcgtatggct cccggcgtcg tctctacggc gacaagttgt acaagctgca ggactaccgc
     2941 ttccgtatcg ccgagatgga aaaggacatc gccgccgcgc gcgctctgac gtacctgtcc
     3001 gcgctgaagc acgaccaggg actatcgaac aacaaggagg cgagcatcgc caagctcaat
     3061 gcaggccagg tcgtgatgcg cgtcaccgaa gccgcctccg taatgctcgg cggcatcggc
     3121 tacacgcagg agagcgtcgt tgagaagctg ttccgagacg cgcgccacac cgcgatcgtc
     3181 gagggcactg acccaacgca caaggaactg atcttcgccg atctcatcag gaagggcggc
     3241 tactgaagcg atgggtcgag aggatgtcgc gcgggatgcc gaggccgcgt tccactacga
     3301 gacctcgaat gacttcttca agctgtggct cgatccgacc atgacgtact catgcgcgta
     3361 ctttgcgacc ggctcggagt cgctcgagga agcgcagatc gcgaaggtgg atctcgcctt
     3421 tgagagggtg aagctgcagc gcggggatcg cctcctcgac atcgggtgtg gctggggcgc
     3481 agcagcggag cgcgctgcag agcggtacgg ggcgagtgcg gtgggactga cggtcagccc
     3541 ggagcagttc gagctcgcgc gtcggcgaga gcgaccaggt ctggcgctca gctaccggct
     3601 ggagggctgg gagacctacc gcgagccgtg cgacgtcatc gtgtcgttcg gcgcattcga
     3661 gcatttcaag gtcgcgaact acggcgcctt cttcgcgcgc tgccacgagc tcctgcctga
     3721 cggcgggtcg atgctcatcc agaccatcac catcgggaaa ccgagcacct cattcaagct
     3781 gtttcgcttc gcccatttcc tgcgcaaaga gatctaccca ggcgctgagc tgccgccgcc
     3841 tgaagtcgtc gtctcgcatg cgcgcctcgg cgggttcgag ctcctccatg ccgagagtct
     3901 tcgtccccat tacgttcgga ccctcgagga gtggctcagg aacctcaagg cgaatcgaga
     3961 cgcggcgatc gccgcaacga gcgaagagat gtacgcgaag aacgttcgct acctcgacgg
     4021 cgcggctcag taccaccgaa cgggtgagac gaacgcgtac cacttccttt tcaggtctgt
     4081 ctgacccact ccgacctcag aaggatcccg tttctcgtca gctcggacaa tcgcgagggt
     4141 tgtcccgggt tgatcagcat gcgcccggga agctctccgt tctcaggaga cagctgccgg
     4201 ctctattccc gagcccgacc gccgatgtgg tagtccctcc atgacgacac cgactggaag
     4261 aacggggccg cctgcgcggc cgagcgcaaa ccggaggatg aggccggttg ggaggctgcc
     4321 taggccgcgt accatcgcag gggatcatcc tgcggatccg agtgttgcga gcgcgggccg
     4381 gaatcccaca cggccctcat cccctgcggc agctgtcagc cttcgggatc cccggtggtg
     4441 acctggatct cctcgtcgca cgcagagcag cggaagaggc gctgaccgcg gatgagctca
     4501 cgctcggggt cgcgtcggtc gggagagtgg gagtcgaccg gcctcatctc cacccagtcg
     4561 ttgccatgcc ggtgcatgat ccggtttgtc tcaaagttca acacgcgcct gatcctccac
     4621 tcgcctctat gaacgttcgg tagtgtcccc gatccgccgg ctatacgcca ccccagaccg
     4681 tgcaaccctg gaccatgcat gcgtgtggca acgcggcacc caatgagctc caaaaaccgg
     4741 tacgctgatc atgccgcctg aatccggtat gccacccacc caacccatcg ggagtctcat
     4801 gcggttctca ctgatccccc gcgaaacgaa gttcttcgac atgttcgtcg aggacgctcg
     4861 caacgtcctg ggcgctgctc gcctgctcga gcagttcttc cgcaactacg acgatcgcga
     4921 gcggctggcg agccagctcc tcgatgcgga gcacgccggc gaccagatga gccacgagat
     4981 cgggcacaag ctggaatcga cattcgtgac gccgttcgac cgcgaggaca tccacgcgct
     5041 gatcagccgc cttgacgaca tcctggacct gattgaggag gtggccgaca cctgcatcct
     5101 ctacaacatc gacgcaccga cgccaccggc gatccagcag gcggagatca tcgtccgcca
     5161 gtgcgagcag ctcgcccaag ggctcgggaa gctccggagt ttcaagggcg tcgagccgtt
     5221 ctggatcgag gtccatcggc tcgagaacga gggcgatcgg atcactcggc aggcggtagc
     5281 cgagctgttc aagcacgacc aggacccgat cgaaatcatc aagtggaagg acgtctacgc
     5341 cctcctcgag aaggcgatcg actcggccga ggacgcggcc aacgtgatcg agcgcatcgt
     5401 cgtcaagcat gcctgagggc ttcaccgcgg ccctgctcgc ggtgttcggc ctggcggttc
     5461 tcttcgacta catcaacggc ttccacgaca ccgccaacgc gatcgcaact tccgtcagca
     5521 cccgagcgtt gagcccccga ctcgccatcg cgatgagcgc caccgccaac ttcgccgggg
     5581 cgctgaccgg gacagcggtg gccaagacca ttggcgcagg tttgatcgca ccgcaggctg
     5641 aaggcgggct ggtcgtcgct gccgccctcg tgggcgcgat cgtgtggaac ctgctgacct
     5701 ggcgcctggg catcccgtcc tcgagcagcc acgcgctgat cggcggcctg ctgggtgcga
     5761 gtgctgcggc ggtgggattc ggcgcctggc aggtcgacgg catcctcggc aaggtgatcc
     5821 tgcccctcat cggatctccg atcgtcggct tcgtcggcgg ctttgccctg atggtggtca
     5881 tcttcaacct gtttcgaagg gcacatccga agaccatgaa tgaccgattc cgccgcctcc
     5941 aggtgctgtc cgcagccttc atggccttca gccatggaag caacgacgcg cagaagacga
     6001 tgggggtgat gacgctggcg cttctctcgg cgggcatcat ccccgaattc aaggtgcccc
     6061 tgtgggtcat catcctggca gccagcgcca tctcgctcgg gacggcagcg ggtgggtggc
     6121 gaattatccg caccatgggc tccaaggtcg tcaagctcga cccagtgcat ggcttcgcgg
     6181 cggagacgac ggctgcgacc atcatcttcg gcgcctcgac gctgggcatg cccgtgagca
     6241 ccacgcatgt catctcgagc gcgatcctgg gggtcgggtc gtcagaccga ttccgcgcgg
     6301 tgcattgggg cgtggcgcgt cgcattggga tcgcctgggt gctcacgctg cccgcctcgg
     6361 ggctggttgc ggcgctcgcc tatgtgctgc tgagaccgat gctcggctga tcccacccag
     6421 ggctagctga agaagacgaa ccggattccc acgaccacca gcagcacgcc gaaggcacgc
     6481 tcgatcacct cgctcggaag cgcaagcccc aaccgtgcgc cgatgaatgc gccaatcagg
     6541 aggcccatcg ccagcaggcc ggcgttaagg acgttgacct ggccggcctg ccagtactgg
     6601 atcgccccga agagaccaac cggcaggagc agggccgcca gggacgtgcc ggcggcctgc
     6661 tgccccgtga agcccaggat caggatcagg agcggcacga tgaccacgcc tcccccgatg
     6721 ccaaagaggc cggacagaac gccgccggcc aggccgatga gcagcatcag ggcgacaggc
     6781 atgggttctc ctccacattc gtcttggtcg ttgatcctga ggcatgggca cgggccttaa
     6841 cacgccgttg acgctgcgct aagcgcgcct taacgggcac gccgaacctt tgtggcgtgc
     6901 cttgccggca gttgctggcc ggcttcatca aaccatggag gaagcattct gtgacctttc
     6961 ggacacagcg gcgcaatgcg ctggtcgtcg tcctcagcag caccctgctg atcgctgcct
     7021 gctcgagcaa caaccgcccc tcgagcaccc ccacgtctac ggcagtcgcc tcggaaggtt
     7081 ctgcgggtcc gtcagctggc gcggttgatc tctcgcaggt gccgccgaag cccgcctact
     7141 cgggcgcgat caccgcggac ggggcgacct tcccgcagcc ggtctacgag cagtggacaa
     7201 cggactatgc gaccgagacc ggaatccaga tcagctacac gggaggcggt tcgggtcagg
     7261 gggtcaagga catcacggcc aacatcgtcc agttcgccgg ctctgacgcg ccgttgaagc
     7321 cccaggagca ggcagccgcc gaggccgcaa acggcccgat cctgcacatc ccgaccgtct
     7381 tcggatcgat cgtgttggcc tacaacgtgg gtggcatctc caagcccctc aacttcagcc
     7441 ccgacgtgat cggcaagatc ttcaccggcc agatcaagaa ctggaacgac ccggcgatcg
     7501 ccgccgataa cgccggtgtc accctgccga acctggccat caccgtctcg catcggaatg
     7561 acggatccgg cacgaccaat gccttcacaa cctggctgtg cgcggtgagc accgactgga
     7621 aggccaaggt cgacccgtgc agcggcaaag aggtcacctg gccggttggc cttggcggca
     7681 agggcaacgc gggcgtcgcc ggcaacatca ccgggactcc gggcgcgatc ggctacgtcg
     7741 agctcgcctt cgcggtccag aacagcctca ccttcggcaa cgtgaagaac aagtcaggcg
     7801 cattcatcac gccgtccctg gaaagcacgg ctgccgcggc aaccttcgac acgattcccg
     7861 acagcctgac cttctcggtg tcggactcat cggtcgccga cgcgtacccg atcgtcacgg
     7921 cgacctggct cctggtttac cagcagcagg acaaggtcaa caccaacgag cagcaggcgc
     7981 aggcagtcgt ccacttcctg atctggaccc tcgacaaggg tggtgcatct gcccaggcgt
     8041 tgggttatgc ggtcctgccg gatgccctcc gcgccgcggc cctggatcgg atcgcaaccg
     8101 tcacctacaa cggcacgccg atcgtcgacg cgctctacaa gtagtttgcg atcccctccc
     8161 agaggggcgt cgcgcggaag gcgtcgcccc tccggttccg tcatacactg ctacgagaac
     8221 cgatgaccgc cgacatcgct ccggtccagc ccgatagcgc aaccgccctg atccgtcagc
     8281 gacgcctcag ccttggcgac ccgctgttgg ggtgggccgt tcgcatcctc tcctggtcgg
     8341 cgttagccgc catcatcctg atgctgttgg atacggcggg gcattctctg aagctgctgg
     8401 agcacacgtc gatcctggac ttcctgttcg ggatcctctg gcaaccggcg agcaccgtct
     8461 acggagcgct gccgttcatc tacggcaccg tcgtcacctc gctgctggcc ctcttcctgg
     8521 cggttcctct cgctctagga gccgcgctgc tcgtgaacga gttcctgccg ctgcgggtcg
     8581 cgtcgccgct cgcctactcg atcgagctgc ttgctgcggt accttccgtg gtctacggct
     8641 tctggggagt gatcgtcctg gtcccggccc tgcggcccct cgagcaggcg gtggccggat
     8701 ccccgctggg gcagatcccg ctcttcagcg ggccggtgcc ggggcccagc tacctcgccg
     8761 ccggcgtgat cctggcgatc atgatcgtgc cgatcgttac ctcggtcgcc cgcgacgcga
     8821 tgcgagcgac gccgcggctg cagcgcgaag gtgcgctcgc actcggcgca acgcactggg
     8881 agacgatccg acacgttgtg ctgccgtttg cgcggggcgg catcttcgcc ggcgtgatcc
     8941 tgggcctgtc tcgtgcgatc ggtgagacga tcgcggtcct cttcgtgatc ggcaatcagc
     9001 cgcagatcaa cgcatccatc ttcgctcccg gctacagcct gccgtcggtc atcgccggtg
     9061 agtttgccga ggcgaaccag ccgttccacc cggaggccct gatcgggctg ggggtcgtgc
     9121 tcttcctgct ctcattcacg gtcaacgtgg cggcgcgcgt cgtcgtcggc cgcttcgagg
     9181 ctcgtgccgg tggcgccgat gttcgctgag cactcgctgc attcgatctc caggagccgg
     9241 cgcagtcgaa accgactcgt ctatctcctc tgcctggtgg ctggtgcgat cgcgctgctg
     9301 ccgctggtcc tgatcctctt ctatctcttg gtcgaaggcc tgaaggcctg gagccctgcg
     9361 ttcctggtct ccccgccggg cggtatcacc agcaagtccg gcggcgccat gaacgccatc
     9421 ctcggcaccc tggagatgac cggtctgggg ctcgcgatcg ccacgccgat gggcctggca
     9481 gtggccctct acgtggcgga gtatcgggag gggcgcctgg cagtgatcgt ccgtacggcg
     9541 gttgacctgg tggcgggcat cccctccatc gtcatcggcc ttttcgtcta ctcgctggtc
     9601 gtcatcacga tggggcattt ctccgggttc gccggtgccg ttgcgctgtc gctcatcatg
     9661 ctgccgatca tcgcccggac cagcgaggag atgttcaaga tcgttcctcg caggctgcgg
     9721 gaagggacca tggcgctggc catgccacgc tggcgcgcca tcgtcgcggt ctacatcccg
     9781 acggtcgcgg ctggaatcgt gaccggcatc gtgctggccg tttcgcgggc ggtcggcgag
     9841 accgcacccc tcctcttcac atccctgggc agcaacttcg tggtcaccga cctgaaccag
     9901 ccgatgtcgg cgcttcccct gtacatgttc cagaacgccc tgaacatcgg cttcccggcc
     9961 gcgcgagagc gcgcgtgggc ggccgccctt acgctggttg cgatcgtcct catcttcaac
    10021 gtgctcggac gctggctcgc cggcaggacg cagctcaagg tgagatagcc agtgcccgtt
    10081 gaggaaccag agatgacaac cgccatcgaa ccaatcgcga tccaggtgca gccccgggcg
    10141 gagcctcgcc ccgatgccgg gggagtcgcg cagactgtgc ccgccatgga tgccgccttc
    10201 gaggtccagc acctgaacgc ctactacgga accaagcagg ccgtgcgcga tgtgaccttc
    10261 accgccggca agcgcgcggt gacggcgatc atcggtccat cggggagtgg caagtcgacc
    10321 gtgctgcgct cactgaaccg gatgctggag gttgttccca cggcgcgaat cgatggccag
    10381 gtcctcttcc aggggatcga catctacgcc cgtgagatcg atcccgcagc ggtccggcga
    10441 acgatcggga tggtcttcca gcagcccaac ccgttcccga cgatgtcgat ctacgacaac
    10501 gtcgcggcag cggctcgctt caacgggctg gtgaagggtc gtgcgcagac tgacgagctc
    10561 gttgaacggt gccttcgcca ggccgccctg tgggacgagg tgagggacac gctctccaag
    10621 tcgggggcgt cgctctcggg cgggcagcag cagcggctgt gcatcgcgcg agcgctggcc
    10681 gtcaaccccg aggcgatcct gatggacgag ccatgttcgg cgctcgatcc gatcgcaacg
    10741 ctccagatcg agcagctggc tcgcgaactg gcgcagtcat atgccatcgt catcgtcacg
    10801 cataacatgc agcaggcagc gcgggtttca gaccagacga tcttcatgag catggacgcc
    10861 gagcgggcag gctacgtggt cgagaccggc gccaccagcc agatcttcac caagccggag
    10921 aaccggctga ccgaggacta catcaccggg cggttcggat gagcgagggc ggaccgatgg
    10981 aaccggcgag caacgcagac gcggcgcgcc acagcgtgcg gcgggggttc gacgaagcgc
    11041 tgaacgcgac caaggatgat gtgctgcggc tgggagcgct ggttgagcgg gcgcttgagc
    11101 gatccggaca tgccttgctc gaacgcgacg tcgaccttgc cgaccaggtg cgacgcgagg
    11161 acgcccaggt gaacgagctg cagcgcagca tcaacaccgg tatcacgacg ctcatcgcaa
    11221 cccaggcgcc ggtagctcgg gacgtccgcg agctgctctc cctgtaccac gccagtgccg
    11281 agctcgagcg gatgggtgac tacgcggtca gcatcgccaa gctcacccag cagctcgcct
    11341 cggagccaga gatccccgtg ctgccccaga tcccgaagat ggagcttctc tgccgggagc
    11401 agctgcggga cgcgatgcgg gccctggtcg acgtcagcga ggagcaggcg cgcgcggtat
    11461 gcgcccgaga cgacgagctg gaccacctct accgccgcgt gtacgaggac agcatgaacc
    11521 tgatggaggc tgcccccgac aaggtccggc aggccaccca catgctcttc atcgcccatc
    11581 acctggagcg gctcggcgac cgggtcacca acatcggcga ggatgtcgtc ttcctggtca
    11641 ccgggcagat cgaggacctg aatccctgaa ccgcccgctt cgcacgtgaa gcagcgcatc
    11701 ctggtggtcg aggatgaccg cacgctgcgg atggccctgg tcttcaacct cgaacgcgag
    11761 ggatacgaag tacgcagcgc ggtcgatggc gagcaggggt tggccgcggc gcgggacggc
    11821 cggctggatc tcatcgtgct cgacctgatg ctgcctgcca tgagcggcct ggaggtgctg
    11881 cgcacgatgc gcgccgaggg gatcgacacg ccggtggtga tcctgtcggc caagggaggc
    11941 gagatcgatc gggtggttgg cctgaaggtt ggggccgatg actacatccc caagccgttc
    12001 agccgcccgg aacttctcgc ccgggtggaa tccgtgctgc gccgacagcg tcgttccgag
    12061 gcggctgggg acgagcggct ggccgaggta cgcagcggcg ccctggcgat cgacctgagc
    12121 cggcacctgg caaccttcag cggggagccg ctgcgtctca cgaccaagga gttcgagctc
    12181 ctcgcccacc tggccgcgca tccgggtcgc atcttcaccc gcgatcagct cctgacgcgc
    12241 atctggccct tcgactttgg cggcgacagc cgaaccgtgg atgtccacat cagctggctg
    12301 cgctcgaagc tgcgcgcgct cgacgggcac aactacttcc gcaccgtgcg cggcgtcggg
    12361 tacgccttcg cgccccgccc agatgtccac tgacaccctc gacgccgcgg cgttcgccgc
    12421 gctcctggag cgcaccgccg gcgacatcct cctggtcgtc gacgaccagc tgcgcatcac
    12481 gcgggtcggc gccggtgctg ctgcgcttgc ccagcgccgg gtagacgagc tgatcggaat
    12541 gagcctgatc gccgccttcg gatccgcgcc gctggatgcc ctggcgcggc atgcggcaag
    12601 ggaccgctcc ccgtcgctcg gcgaggcgga gctgggaccg cgcggcgagc gcgcatttgc
    12661 cgtcgacgcc gttccccttg ccggcggaga gctggtcctg gcgttgcacg agatcaccgc
    12721 tctccgccgg gccgagcgcg tgcgccgcga cttcgtgacc aacatcagcc acgagctgcg
    12781 cacgccgctt gcctcggtca agctcctggc cgagaccctc tccgccggtg cggtcgagga
    12841 cgcagccacc gccggccagt tcgcgatcca gatcgagcgc gaggtggacc acctggcaca
    12901 gctcgtggcc gagctgctgg acctgtccat gatcgagtcg ggtgaaactg cgctgcggct
    12961 ggagccggtc gacccgagca ccgtgatgcc ggacgtggcc gagcgaatcg ggccggtcgc
    13021 agcacgccgc ggcatcgccg tccagctgct ccctgggcct gatggtgccg aggtccgggt
    13081 caacgccgat gcgggccggc ttggtcaggc actcctcaac cttgcccaca acgcggtcaa
    13141 gtacaaccgg cctgggggcg aggtgcggct gggctgggag ccgtccccag gcggcgtgcg
    13201 gttcacggtc gccgacgacg ggatcgggat ctccggggcg caccagcagc ggatcttcga
    13261 gcgcttctac aaggtcgacc gctcccgggc gcaggagtcg gacgacacga ccggcggggg
    13321 cagtgccggt ctcggactcg ccatcgtgcg gcacatcgcg gaggcgcatg ggggctcggt
    13381 cggcgtgcga agcgtcgagg gcgagggctc catcttctgg gtcgagctgc cgttggcgga
    13441 gcgcgcctga tatagttgtc gggtcaacca tgagcacaca agacacgatc cccggcccac
    13501 gcgacccgcg cctcgagacc tggcgttcgt tcctggtcgc gcatgcccag atacgccgcc
    13561 agctcgaacg tgagctgcag gccgagcagt cgctcggcct cggcgagtac gaagtgctgt
    13621 tgctcctcgc acgcgccgat gcccggcagc tgcgcatgag cgacctcgcg gaccgcctcg
    13681 tcctgtcccg cagtggcgtg acgcgcctcg tcgatcggct ggaggcggag gccctcgtcg
    13741 agcgcacgtc gtgcactacc gaccggcgcg gctcctgggc gacgctgacc gacgccggcc
    13801 accggcggct acggcgcgcc tcccccacgc accttcgcgg ggtgggagcg catttcctcg
    13861 accgcatccc gcctgccgag ctcgacgccc tgcagcgcac cctcgaccgg gtcctcggcg
    13921 aggcctagcc cccgggcgct cggcttgctc ccggcagcgc tccggccgcc tccgcacgca
    13981 ggctgctgac catggacgcc cgatcgtcgt gaggcgcgag ctgcaggaat cgatcgaact
    14041 cgcgcaccgc cgtggcgttg tccccgcgtc ggaaggcgat cagcccgcgg tagaacggga
    14101 tgtccggctc gtcagggttg gcaatggtgg cgtatgaatc ggtcgtcgcc tgcgcgtcgt
    14161 cgtagtgggc cgtcacgagg tacgcgttga tcaggcgccg ataggccgag tggttctccg
    14221 gttgcagcgc gagcaggatc tggagcgcga ccgccgctcg ctgcaggtcc tcctgggagt
    14281 cgcccgccag gtaggcatcg gccaggtcgg aggcgacctg tgcgtcgcgc gggttcgtgg
    14341 cgaagaggtc gagcaggcgc tggatctccg cctgacgatc cgcctcgtgg gacatggcat
    14401 cggcgagcgc ctggttcacg accgtgcgct gggccaggcc gatggagccg ggcagcgcga
    14461 agccgaccag catggtgagc gcaatcagcg ccccgagtgc cgcgatcgac cctcggcccc
    14521 gtctcggcgc cacgacgcgt ggcgggggct cgaccggggc cggctcaagc gtctccaccg
    14581 cccgcgcctc ggcctcggag cgctcccgct catacgaggc atcgtcgagc gagccggccc
//
LOCUS       DATCQR010000019         2301 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_63222, whole genome shotgun sequence.
ACCESSION   DATCQR010000019 DATCQR010000000
VERSION     DATCQR010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63222"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="VIN32_00750"
     CDS             complement(<1..329)
                     /locus_tag="VIN32_00750"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013419847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase"
                     /protein_id="HEY8921137.1"
                     /translation="MFSFGRKSQMPDPADALPGRFEPLPTAERHLINGQPLKGPYPAE
                     AQVIDFALGCFWGAEKDFWQLPGVIVTAVGYQGGFTPNANYSEVCSGRTGHAESVRVV
                     YDPERVSV"
     gene            480..>2301
                     /locus_tag="VIN32_00755"
     CDS             480..>2301
                     /locus_tag="VIN32_00755"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY8921138.1"
                     /translation="MTAPPADSVPAVAAPPRTPRPPRRWLIPSIEVTVLVVAGGLVLA
                     TALAVSANASSNLEAGATAEAVSAVKAVVHAYVDPLLSTDLLTSPDAVTAATINQQLE
                     LLTGSRSILRIKVWSPKGTVVFSDLPALRGRTFAISADLAEALEGHIGADIGNAVADE
                     NVFELAFTPKLLEIYLPIGADGEKPVGAYEIYQDATKLEALLATTRQQIFLIVGGIGM
                     VLLVTLYLAFHGAGRLVAELSGRLRRSETRFRSLVQNSSDLVVLLDRESRMVFVSPAI
                     ERILGEPAAMRTDQPVTELVHAEDAEWITALLERLAATPGSVSGGEVRMHHADGRWRW
                     IEVVGTNLMSDPDVRGIVLNCRDVTDRRTLEEQLRHQAFHDPLTNLANRPLLVDRIEH
                     ELEGRPMESPATTAVLLLDLDDFKTVNDSLGHAAGDELLAAVADRVRATLRGRDTAAR
                     IGGDEFGVLIRTGERAEVEAIAERILASIREPIDLGDHHLTVAASMGIAYADGRQATA
                     DELLRSADVAMYMAKARGKDRFVVFEASMHDAAVRRLRLKADLEHGLEAGQFHLLYQP
                     IIDLTTGGPVGLEALLRWNHPSWMIGPSEFVPFAEETGMII"
BASE COUNT          371 a          776 c          780 g          374 t
ORIGIN      
        1 acgctgaccc gctcggggtc gtagaccacg cgcacgctct cggcatggcc ggtgcgtcct
       61 gagcagacct cgctgtaatt ggcgttgggc gtgaagccgc cctggtagcc gacggcggtg
      121 acgatcacgc cgggcagctg ccagaagtcc ttctcggcgc cccagaagca gcccagggcg
      181 aagtcgatga cctgggcctc ggcgggatac gggcccttca atggctgtcc gttgatgagg
      241 tggcgctcgg cggtgggtag gggctcgaag cggccgggga gcgcgtcggc tggatcgggc
      301 atctgggact tgcgtccgaa gctgaacatg cgccaattct atgggaggtg cggcagggtc
      361 gggtgaaggc ccgatgaagg ttgcgtgacc ctggcaggac catcggccta tcggggatcg
      421 tttcgcgttc aggtaggttc gcattcctaa gaatttgcag gaaccgagcc cctgatttga
      481 tgaccgcgcc tccggctgac tcggtccccg cggtggccgc accgccgcga acgccacgac
      541 ccccgcgacg ctggctgatc ccctccatcg aggtcaccgt gctggtggtc gcaggcggcc
      601 ttgtgctcgc caccgccctc gcggtatcgg ccaacgccag cagcaatctc gaagccggcg
      661 ccacggcaga ggctgtctcc gccgtcaagg ccgtcgtgca cgcctatgtc gaccccctgc
      721 tttcgacgga cctgctcacc tcgccagacg ccgtcacggc ggcaacgatc aaccaacagc
      781 tcgagctgct gacggggagc cgcagcatcc tgcgcatcaa ggtctggtcg cccaagggga
      841 cggtcgtctt ctcggacctg cctgcgctgc gcggccggac attcgccatc agcgccgacc
      901 tggccgaggc gctcgagggc cacatcgggg ccgacatcgg taatgccgtc gccgacgaga
      961 acgtcttcga gctggccttc actccgaagc tgctggagat ctacctgcca atcggcgccg
     1021 acggcgagaa accggtcggc gcgtacgaga tctaccagga cgcgaccaaa ctcgaagcac
     1081 tcctggcaac gacgcgacag caaatcttcc tgatcgtggg tggcatcggc atggtgctcc
     1141 tggtcactct gtacctggcc ttccacggcg ctggtcggct cgtggcggag ctgagtgggc
     1201 gcctgcggcg cagcgagacg cgattccgct ccctcgtcca gaactcatcg gacctcgtcg
     1261 tgctgctcga tcgtgaatcg cggatggtct tcgtcagccc ggcgatcgag cggatcctgg
     1321 gggagccggc ggcaatgcgc accgatcagc cagtgaccga gctggtccat gcagaggatg
     1381 ccgagtggat caccgcgctg ctcgagcgcc tggcggcgac gccgggctcg gtatcgggtg
     1441 gcgaggtccg catgcaccac gcggatggac gctggcgctg gatcgaggtc gtcggcacca
     1501 acctcatgag cgaccccgac gtgcggggca tcgtcctgaa ctgccgtgac gtgacagatc
     1561 gccgcacgct ggaagagcag cttcgccacc aggccttcca cgacccgctc accaacctgg
     1621 ccaaccgtcc gctgctcgtt gaccgcatcg agcacgagct ggaggggcgc ccaatggagt
     1681 cgcccgccac gaccgcggta ctgctgctgg atctcgacga cttcaagacg gtcaacgaca
     1741 gccttggcca cgcggccggc gacgagctcc tggcggcagt cgccgaccgg gttcgcgcca
     1801 cgctccgtgg ccgcgacacg gcggcccgca tcggcggcga cgaattcggg gtcctgatcc
     1861 gcaccgggga gcgcgccgag gtcgaggcga tcgccgagcg catcctggct tccattcgtg
     1921 agccgatcga ccttggcgat caccacctga cggtggccgc ttcgatgggg atcgcctacg
     1981 cggacggccg ccaggcgacc gccgacgagc tgctgcgaag tgccgatgtc gcgatgtaca
     2041 tggccaaggc gcgcggcaag gaccgcttcg tggtcttcga ggcgagcatg cacgacgccg
     2101 cggttcgccg cctgcggctg aaggccgacc tggagcacgg tctcgaggcc gggcagttcc
     2161 acctgctgta ccagccgatc atcgacctga cgacgggcgg tccggttggg ctcgaggcgc
     2221 tgctgcgctg gaaccaccca tcctggatga tcgggccctc cgagttcgtc ccctttgcgg
     2281 aggagaccgg gatgatcatt c
//
LOCUS       DATCQR010000020        12735 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_78400, whole genome shotgun sequence.
ACCESSION   DATCQR010000020 DATCQR010000000
VERSION     DATCQR010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 12735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12735
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78400"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..826
                     /locus_tag="VIN32_00760"
     CDS             <1..826
                     /locus_tag="VIN32_00760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921139.1"
                     /translation="PYRPPASETLPADLLRIDVNDYRNEASLSPGPILVIGSGQSGCQ
                     IAEELVEAGREVVLSCGRAAWVPRRVGGRDIFRWLVDTGFMTEPVGSLPSPAARLIAN
                     PLATGHGGGHDLNLRMLRGKGVTLVGHFLGASDRDGRFAADLGESVAWGDERYGLLMD
                     GIRRTAAELGHADPEINEPEPFDPGAPEQLSLEGLGSVIFAGGFRPSYQSWLPWPAAF
                     DEMGFPIQQDGASTVVDRLYFVGVHWMRQRKSALLLGVGEDAAIVAHAISGRRPTV"
     gene            897..2039
                     /locus_tag="VIN32_00765"
     CDS             897..2039
                     /locus_tag="VIN32_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017617261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HEY8921140.1"
                     /translation="MPFDMSLVQRGARLRQTPFYEAEQAYGPRGYTVYNHMLFPINFD
                     DFEAEYWHLLHHVTLWDVAVERQLEVQGRDGFRFAQLLTPRDLSKCAVGQGKYVLITT
                     PDGGIVNDPVMLRLGENHFWFALASSDALLYAIGLAADLGWDVQLSEPDVSPLQIQGP
                     RSREVVQALFGDEIRSLKYYWFRELEVDGIPVIVTRTGWTAEVGYEVYLRDGQYGTAL
                     WERIMEAGRPFQIRPTGPVDIRRVEGGILNWGADMTIENNPFEVGLERLCSLDGAFKF
                     QGKAALRRIRAEGVSRRLVGVEIGGEKLGMNFTKWPVHASDAPVGEVTTALWSPRLEK
                     NIGYAWLPVELAGNGTSVTVSTPDGDRPATVVPMPFLDPTKEIPKS"
     gene            complement(2118..3971)
                     /locus_tag="VIN32_00770"
     CDS             complement(2118..3971)
                     /locus_tag="VIN32_00770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HEY8921141.1"
                     /translation="MFKSSVRNRLFVLIAGTAVLASLSIGVAYLATETERLDARAETH
                     TVADLYGLTVLLSNSIRDQDEAVGNYLLASAPEALDRYAGALENELHLAERMRLAAAD
                     DPDIVAALEPLEARSRAWRATFAAPAIAAVSAGSPAQVARFVETVYADKEPTLTGIYD
                     LVSRISETEASAAAHDDALTRTRALANVFGIALMLLAAAASLGMARRWVTRPLDKLLT
                     TAADVESGANVAFVTERNDEIGKLGSALERMRFALQQDAGRSGILNRFTEVTTFAADD
                     EAVAVASLEALRMLVQPDAAVSHVLNRSKDRAVPEAKIGSPIAEVLPLNSLSRCPAIV
                     RGSIYVTLDAAQPLSVHCPIYPVDDGTLACIPLAHGETVGSIHLFWERPNAFRLDQRS
                     IVTRIAEHAALAISNRRLMAALQGMASTDPRTGLSNTRAFDQMLEDALRARRDDETIA
                     VLMLDLDHFKEFNDRYGHPAGDEALRAFADILRSCLRDGDVAARYGGEEFAIFLPTVD
                     QMTALTIAERIRSRTESTLIALAPGISDRITVSIGLASAPHQANDRIALLRLADEALY
                     QAKEAGRNQVVTAGASTADQAALRPLASELPLPTVVSPRRLRRRVRTAAES"
     gene            4148..5197
                     /locus_tag="VIN32_00775"
     CDS             4148..5197
                     /locus_tag="VIN32_00775"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEY8921142.1"
                     /translation="MAQLDLNPLPGSPSIVVAEDDAATRALLRVVLERAGYEVRQTDN
                     GADALAEILRQPPVVALLDIGMPEMDGLEVTRRLRSEPLTALLPIIMVTARGRLEDKV
                     AGLDAGASDFITKPFEPAELLARVRSNQRLATALTKLENTRDVLVALASAIDAKDPVT
                     EHHCGRVAELATSLAGVAGLSEEAIEAVAYGAVLHDVGKIGIAEAVLRKAGELTDVER
                     TEMQRHPVIGAEILSPLRLGVLIGPIVRGHHEWWDGTGYPDHLSGEAIPVGARIVSVV
                     DAYDAMTHARPYRAALSLDEARAELVRCRGIQFDPEVVDLLLGHLDSVVEPLQAVSES
                     TPALHVLHGEAERWT"
     gene            5188..7836
                     /locus_tag="VIN32_00780"
     CDS             5188..7836
                     /locus_tag="VIN32_00780"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8921143.1"
                     /translation="MDLSSLVVSVLFGILFISAVVGYVRRRDALSRDVMLIFGTVAML
                     FVVVGARVVIGSLPAWVDGAALALLFAQPLLTLRLANKLHPVHRPVRLAATVAYLATA
                     LPLIVFPLKAVVVLVLAAIAVFVVTDLVASFYLAREARRRVGSARVRLGAAAAASALM
                     ALAVLVSTIVSSIPAIGTVGADIGRFLGLAAASLYVVAFMPPRWLRNIWGAVASFDFE
                     QDLMGVAADPSDAAVWQRLADASRRATGSTATLVVTGELGSALVVASSAEGSGRDQVA
                     ATRFDSVPTASDEHRPTREGSLADQAHAIGAPFVSVLPFTLAGGEPGALLLLRARPSL
                     FASDDHAMLRSLVGRAAIFAERSRAVTEQAALAQRLAQTVAALEQASQAKSDFLASMS
                     HELRTPLSAIIGFSALMRDEPLADDRRSVPDEWIEHVHRSGDHLLALINDVLDLTKIE
                     AGRINLELEAFGLGTALGESVEGLRPLAQRKSIEMVLDCEDGMINADRGRLRQMVYNL
                     LSNAIKFTPDGGHIGVEGRWVGDEARITVRDTGVGIEPNDLEHIFEEFRQVGDLKARQ
                     AGTGLGLALCRRLAEAHGGRLEVTSEIGRGSRFTVVLPGTRAESSDRPSPPPPIAVEV
                     TGPSILIIEDDPGAVRLLRAYLEAEGYNVEVATDGETGIAAAQTNPPDAIILDVLLPG
                     IDGWEVLRRLKGDPALRDLPVVVVTVVDERNVAMNLGAVDYFLKPVRREALLARLSQY
                     TFTTKVKVGRVRVLAIDDDPAARELVANALRPEGFDVVSAASGREGLDLALEDPPDLV
                     ICDLLMPDMDGYEVVERLHANESTKDVTILILTGQDLSAADRERLNGKVAEVVRKGVD
                     PRPAFARWLRRASAAARRRSAEALTS"
     gene            complement(7852..8490)
                     /locus_tag="VIN32_00785"
     CDS             complement(7852..8490)
                     /locus_tag="VIN32_00785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921144.1"
                     /translation="MRAVVGRHRAVAAILVAGLMLAACTTSGQPLKQPSWPAPADPMT
                     LTTKAGLEATQSEHLNSHTHAHLHVFVDGNDVVVPSGIGIDIAAPAGIDQKKTADGTA
                     TEYYVTLCFAPCLSPLHTHDPSGIIHTESKKDNQAPYTLGQFFTEWGVPLDDQCVGEF
                     CKSDTSVAVYLDGKKYEGNPADIELKTHLEVAIVIGKPPSVIPDTWDFGPEP"
     gene            complement(8531..9013)
                     /locus_tag="VIN32_00790"
     CDS             complement(8531..9013)
                     /locus_tag="VIN32_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005250087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY8921145.1"
                     /translation="MTSAQPQAQPVTLTMPSDRELRIERVFNAPRDRVFAAYTDPALI
                     PQWWGAETVVEEMDVRTGGSWRFVAHYPNGYEAAFSGTYREVTPPEKIAQTFSVEGMG
                     TTHVETITFGDLGERTRLTVTSLFDTTEQRDSIVASGAERGANETYARLEALLASLAA
                     "
     gene            9176..9856
                     /locus_tag="VIN32_00795"
     CDS             9176..9856
                     /locus_tag="VIN32_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921146.1"
                     /translation="MLHRILPALAVVTMLALPAAASAATPAIYHTIDGRFASAYFTSL
                     DGCLQTDVWVSTSAATYAPQPGIRDAVNKQGRTSVEIFVYDTCQPMDGKHYPIVADWF
                     AQGSDRLVAESRMRSAHLAANLKMTDSVSGDSAVVPIDLLWQATAVAHPDTVPNNHVR
                     FPGEGIVNTHDNNINLSAVASGTVLLGGVNVSADPDPSAILEQIRSACMEIGYPHWDG
                     DTLFCFGF"
     gene            9890..10555
                     /locus_tag="VIN32_00800"
     CDS             9890..10555
                     /locus_tag="VIN32_00800"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017556735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEY8921147.1"
                     /translation="MARALIFDLDGTLIDTVYAHVFAWQRALAEAGLPIEGWRIHRRI
                     GMSGGLFTRAVARELGRPLSDTEVEEIQRRHGELFRELVPERRPLPGAVDLLAHLREA
                     GVPFGIATSGRRPEIDASLEVLGVGADVVVVERGDVLRAKPEPDLFLACQERMGVHIR
                     DVTVVGDAVWDLLAARRAGMLSVGLLTGGYGEDELARAGAFRVYRDPAELNASLDELG
                     IVP"
     gene            10561..11073
                     /locus_tag="VIN32_00805"
     CDS             10561..11073
                     /locus_tag="VIN32_00805"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8921148.1"
                     /translation="MAVTLRRWGPGDRALVDRVMGDPRMTEHLGGPESPEKLAERQAR
                     YERIHEAGTGGMFVICAGPEAEGVGSVGYWEDAWRGGTVWEIGWSVVLEFQRRGIASR
                     AVGLLLEEIAAEGLDRPILAYPAVENEASNAVCRKAGMTLIGEAVVEFPPGNPFRANE
                     WILQPASARS"
     gene            complement(11049..12233)
                     /locus_tag="VIN32_00810"
     CDS             complement(11049..12233)
                     /locus_tag="VIN32_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEY8921149.1"
                     /translation="MTERGIASSLTQYEATPGVIDLAWGHPDPSLLPVRELADAARRA
                     MNRHGSDILEYGSTAGSPPLIDFICERLAETDARAPSPAQVVITSGASGGLDLVATLL
                     LSAGDTVIVDVPTYHLAISILADHPVRLEAVASDADGVRLDDLADTVARLRRAGERPR
                     LLYTIPTFHNPTGRKVSLERRNALVAFAADESLLIAEDDTYRELSYDGPPPPSLWALD
                     GAGSVIRLGSFAKSVAPGLRLGYVTSSSEIATRLATGGLLDSGGGMNHFAATVLAEYA
                     ANGDYARQVERFRSAYRAQRDRLLAGLGAHLPDTASWSRPEGGYFVWIELPPGITADE
                     LLPVAAARGMAFLPGHRFFLDDRAAPGALRLAFSMHPAETLEEATADLGAAIDQLRAE
                     AG"
     gene            12334..>12735
                     /locus_tag="VIN32_00815"
     CDS             12334..>12735
                     /locus_tag="VIN32_00815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921150.1"
                     /translation="MDTTRSPLQADLVDMLDALRNAERDLFAMVPADQREAAGTIGEW
                     SPKDLLAHLGAWRAVEARRLEARASGGAASPDDPGLDEPIDESNARLQARDRDLSWGT
                     VAAEADTSIDALITAVDRSSTDALCECDDASV"
BASE COUNT         1923 a         4253 c         4502 g         2057 t
ORIGIN      
        1 tccctaccgc ccgcccgcct ccgagacgct gccggcggac ctgctgcgga tcgacgtgaa
       61 cgactatcga aatgaggcct cgctgtcgcc ggggccgatc ctggtcatcg gtagcggtca
      121 gtccggttgc cagatcgccg aggagctggt cgaagccggc cgggaggtcg tcctctcctg
      181 cggcagggcg gcctgggtcc cgcgacgggt tggcggtcgg gacatcttcc gctggctggt
      241 cgacaccggc ttcatgaccg agccggtcgg ctccctgccg agtcctgcgg cgcgcctcat
      301 cgccaacccc ctcgccaccg gccacggcgg cggccacgac ctcaacctgc ggatgctgcg
      361 aggcaagggc gtgaccctcg tcggccactt ccttggcgcg tcggatcgcg atgggcggtt
      421 cgccgccgac cttggggaga gcgtcgcgtg gggggacgag cgctacggac tcctcatgga
      481 cgggattcgg agaacggctg ctgagctcgg ccatgccgat ccggagatca acgagcccga
      541 gcccttcgac ccgggggcgc ccgagcagct gtcgctggag ggcctcggct cggtcatctt
      601 tgccggcggc ttccgcccgt cctaccagtc gtggctgccc tggccggcag cgttcgacga
      661 gatgggattc ccgatccagc aggacggcgc cagcaccgtc gtcgaccgcc tctacttcgt
      721 cggtgtccac tggatgcggc agcgcaagtc ggcgctcctg ctgggggtcg gcgaggacgc
      781 ggcgatcgtg gcgcacgcga tcagcgggag gcggccaact gtctgattcg gctctatctg
      841 cgcccaactg tgggagtagg atcggccccc cgacctgaac cgcctggagt gtccagatgc
      901 ccttcgacat gagcctggtg cagcgcggcg cacgcttgcg ccagacgccg ttctacgagg
      961 ccgagcaggc atacggcccg cgcggctaca ccgtctacaa ccacatgctc ttcccgatca
     1021 acttcgatga tttcgaagcc gagtactggc acctgctcca ccacgtcacg ctctgggacg
     1081 tcgcggtcga gcgacaactg gaggtgcagg gccgcgatgg cttccggttc gcccagctgc
     1141 tgaccccgcg cgacctgtca aagtgcgcgg ttggccaggg caagtacgtg ctgatcacga
     1201 cgccggacgg ggggatcgtc aacgacccgg tcatgctgcg actgggggag aaccacttct
     1261 ggttcgcgct cgccagctcg gacgcgctgc tgtacgcgat cggcctggcg gcggatctcg
     1321 gctgggacgt gcagctttcc gagcccgatg tctccccgct ccagatccag ggtccgcgct
     1381 cccgagaggt ggtccaggca ctcttcggcg acgagatccg gtcgctgaag tactactggt
     1441 tccgcgagct ggaggtcgat ggcatcccgg tgatcgtgac tcgcaccggg tggaccgccg
     1501 aggtcgggta cgaggtctac ctgcgcgacg ggcaatacgg cacggcgctc tgggaacgca
     1561 tcatggaggc gggccggcca ttccagattc ggcccaccgg tccggtcgac attcgacgcg
     1621 tcgagggcgg gatcctcaac tggggcgccg atatgaccat cgagaacaac ccgttcgagg
     1681 tgggtctcga acggctctgc agcctggacg gcgcattcaa gttccagggc aaggccgccc
     1741 tccgccggat ccgcgccgag ggggtcagcc gccgtctggt gggtgtcgag atcggcggcg
     1801 agaagctggg gatgaacttc acgaagtggc cggtccacgc ctccgatgcg ccggttggcg
     1861 aagtcaccac ggcactatgg tcaccgcggc tggagaagaa catcgggtat gcctggctgc
     1921 cggtcgaact ggcggggaac ggcacctcgg tcacggtcag cacgccggac ggcgaccgtc
     1981 ccgccacggt ggtgccgatg ccgttcctcg atccgacgaa ggagatcccg aagtcctgag
     2041 ctgctgctgg gtgaccggcg ccaaggtccc cggagaatgg agctcttcga ggcgtcagcc
     2101 ccgtagggtg ccggagctca cgattcggcg gcggtccgca cacgccgtcg gaggcgccgt
     2161 ggcgagacga ccgtgggcag cgggagctcc gacgcgagtg ggcgaagggc tgcctgatcg
     2221 gcggtggaag ccccagcggt gacgacctgg ttgcggcccg cttccttggc ctggtacagg
     2281 gcctcatccg ccaggcgcag cagggcgatt cgatcgttgg cctggtgcgg cgccgacgcg
     2341 aggccgatgg aaacggtgat gcggtcgctg attcccggcg cgagagcgat cagcgtgctc
     2401 tccgttcggc tcctgatgcg ctcggcgatg gtgagcgccg tcatctggtc caccgtcggc
     2461 aggaagatgg cgaactcctc gccgccatat cgggctgcga cgtccccgtc gcgcaggcac
     2521 gagcgcagga tgtcggcaaa ggcacgcagc gcctcgtcgc ccgcaggatg cccgtagcgg
     2581 tcgttgaact ccttgaaatg atccaggtcg agcatcagga ccgcgatcgt ctcgtcgtcg
     2641 cgtcgcgccc tgagcgcatc ctcgagcatc tggtcgaagg cgcgcgtatt ggagagaccg
     2701 gtcctcgggt cagtgctggc catgccctgc agcgccgcca tcaggcggcg gttgctgatc
     2761 gccagggccg catgctccgc gatccgcgtc acgattgatc gctgatcgag ccggaaggca
     2821 ttcggacgtt cccagaagag atggatggag ccgaccgtct caccgtgagc caacgggatg
     2881 caggccagcg tgccatcgtc gacggggtag atcgggcagt ggacgctcag cggctgtgcc
     2941 gcgtcaagtg tgacgtagat gctgccgcga acgatggccg ggcatcgcga caggctgttc
     3001 agcggaagca cctccgcgat cggcgagccg atcttggctt cagggacagc gcggtccttg
     3061 gagcggttga ggacatgact aacggctgcg tccggctgga cgagcatcct gagggcctcg
     3121 aggctggcga ccgccacggc ttcatcatcg gcggcaaagg tggtgacttc ggtgaagcgg
     3181 ttgagaatgc cggagcgacc cgcgtcctgc tggagcgcaa agcgcatccg ctccagcgca
     3241 gatcccagct tgccgatctc gtcgttgcgc tcggtcacga aggcgacgtt ggcccctgac
     3301 tcgacgtcgg ccgccgtggt gagcagcttg tccagcggac gggtgaccca gcgcctcgcc
     3361 atgcccaggc tggccgcggc ggcgagaagc atgagcgcaa tcccgaagac gttcgccagc
     3421 gccctcgttc gggtgagagc atcgtcgtgc gcagcagccg aggcctcggt ttcggagatg
     3481 cgggagacca ggtcgtagat gccggtcagc gtcggctcct tgtccgcata gaccgtctcg
     3541 acgaatctcg caacctgggc tggtgatccg gcggacaccg ccgcaatggc tggtgcggca
     3601 aaggttgcgc gccacgcacg gctgcgcgcc tccaggggct cgagggcagc cacgatatcc
     3661 ggatcgtcgg cggccgccag cctcatccgc tcggccaggt gcagctcgtt ctcgagcgca
     3721 ccggcgtagc ggtcgagggc ctcgggcgcc gaggccagca ggtagttgcc gacggcctcg
     3781 tcctggtcac ggatcgagtt ggacagcaga actgtaaggc cgtagagatc tgcaaccgtg
     3841 tgcgtctcag cgcgggcatc gagacgctcg gtctcagtcg ccaggtacgc cacgccgatc
     3901 gagagcgagg cgagcactgc tgtccctgcg atgagcacga agaggcgatt gcgaacggat
     3961 gacttgaaca ctgatctgaa acgctagagg aggatcgtcg aacgggatag gacccggtcg
     4021 taccggaaca agtaccgttt ccgagcgcat ttgagcccgt ttggccgccg ggcaaccccc
     4081 ggccagccca taggtaccac tgacatccct cccgcggttg tgcacattgg caccaggagg
     4141 gacccgtatg gcccagctcg acctgaatcc tctccccggt tcgccgtcga tcgtcgtcgc
     4201 cgaagacgac gccgccacgc gcgcgttgct gcgtgtcgtg ctcgagcgag ccggctacga
     4261 ggtgcgtcag accgacaacg gagccgatgc gctggcggag atcctgcgcc agccgccggt
     4321 tgtcgccctg ctcgacatcg ggatgccgga gatggacggc ctcgaggtca cgcgcaggtt
     4381 gcgcagcgag cccttgaccg ctctcctgcc catcatcatg gtcaccgccc gcggccgcct
     4441 cgaggacaaa gtcgcagggc tggatgcggg agccagcgac ttcatcacca agccgttcga
     4501 gcctgccgag ctgctcgccc gcgtccggtc gaaccagcgg ctggccaccg cattgacgaa
     4561 gctggagaac acgagggacg tcctcgtggc cctggccagc gccatcgatg ccaaggatcc
     4621 ggtcaccgag caccactgcg gtcgcgtcgc tgagctggcg accagccttg ctggcgtggc
     4681 gggcctgagc gaggaggcga tcgaggcggt cgcctacggc gccgtgctgc acgatgtcgg
     4741 caagatcggc atcgccgagg cagtccttcg caaggcgggc gagctgaccg acgtcgaacg
     4801 caccgagatg cagcgccacc cggtcatcgg cgccgagatc ctcagtccac tgcgcctcgg
     4861 agtcctgatc ggcccgatcg ttcgcggtca ccacgagtgg tgggacggca ccggctatcc
     4921 cgaccacctg agcggggagg cgatcccggt tggagcccgc atcgtgtcgg tcgtggatgc
     4981 ctacgacgcc atgacgcacg cccgcccgta ccgcgccgcg ctatccctcg atgaggcgcg
     5041 agccgagctg gtgcggtgcc gcggcattca gttcgaccca gaggtggtgg acctcctgct
     5101 cggccatctg gacagcgttg tcgagccgct ccaggccgtc agcgagtcca cgccggcgct
     5161 gcacgtcctc catggcgagg cagagcgatg gacctgagct ccctcgtcgt atcggtcctg
     5221 ttcgggatcc tcttcatctc cgcggtggtt ggctacgtcc gtcgccgtga cgcgctgagt
     5281 cgcgacgtga tgctcatctt cggaacggtc gcgatgctct tcgtggtggt cggggcacgt
     5341 gtcgtgatcg ggtcgcttcc ggcctgggtg gacggcgctg cgctggccct cctcttcgcg
     5401 cagcccttgc tcacgctgag gctggcgaac aagctccatc ccgtgcaccg accggtgcgc
     5461 ctagccgcca cggttgcgta cctcgcgacg gcgctacccc tgatcgtgtt ccccctcaag
     5521 gcggtggtgg tgctggtcct ggcggccatc gccgtcttcg tggtcacgga ccttgttgcc
     5581 agcttctacc tggcccggga ggcaaggcgc cgggtcgggt cggcccgcgt tcggctggga
     5641 gccgcggccg cggcgagcgc cctgatggcc ctggccgtcc tcgtctcgac catcgtctcc
     5701 agcatcccgg ccatcggcac ggtgggtgct gacatcgggc gcttcctcgg cctcgctgcg
     5761 gccagcctgt acgtcgtcgc cttcatgccg cctcgctggc tgcgaaacat ctggggtgcg
     5821 gttgccagct tcgacttcga gcaggacctg atgggggtcg cggccgaccc cagcgacgcc
     5881 gccgtctggc agcggctcgc tgacgcctcg cgccgcgcta cgggatcgac ggcgacgctg
     5941 gtggtcaccg gcgagctggg gtccgccctg gtcgtcgcga gcagcgccga aggctccgga
     6001 cgagaccagg tggcggcgac gcggttcgac tcggtcccga ccgccagcga cgagcatcgc
     6061 ccgacccgcg agggaagcct cgccgaccag gcacacgcga tcggcgcgcc gttcgtatcg
     6121 gtgctgccct tcacgctcgc cggcggcgaa cccggtgccc tgctgctcct tcgggcgcgt
     6181 cccagcctct ttgcgtctga cgatcacgcc atgctgaggt cgcttgtggg ccgagccgcg
     6241 atattcgcgg agcggagccg ggcggtcacg gagcaggccg cgctcgcaca gcggctcgcc
     6301 cagacggtgg cagccctgga gcaggcaagc caggcgaaga gcgactttct ggccagcatg
     6361 agccacgagc tgcgcactcc gctgagcgcg atcatcggct tctcggcgct tatgcgcgac
     6421 gagccgctgg ccgatgacag gcgcagcgtc cccgacgagt ggatcgagca cgtgcaccgc
     6481 agcggcgatc acctgctggc cctgatcaac gacgtgctgg atctcaccaa gatcgaggcg
     6541 gggcgtatca acctcgagct ggaggccttc ggcctgggca cggcgcttgg cgaatcggtc
     6601 gagggtctgc gcccgctcgc ccagcgcaag tcgatcgaga tggtgctcga ctgcgaggac
     6661 ggcatgatca atgccgatcg cggccggctg cgacagatgg tctacaacct gctctccaac
     6721 gccatcaagt tcacgccgga tggcggccac atcggggtgg aggggcgctg ggtcggcgac
     6781 gaggcgcgca tcacggtccg cgacacgggg gtgggcatcg aacccaacga cctcgagcac
     6841 atcttcgagg agttccggca ggtgggcgac ctcaaggccc gacaggcggg aaccggcctt
     6901 ggcctggccc tctgccgtcg gctggccgag gcccatggcg gacgcctgga ggtcacatcg
     6961 gagatcgggc gcggctcgcg cttcacggtc gtgctgccgg gcacgcgcgc cgaatcttcc
     7021 gatcgaccgt ctcctccgcc gcccattgcg gtcgaggtga cagggccgag catcctgatc
     7081 atcgaggacg accccggtgc ggttcgcctc ctccgcgcct acctcgaggc cgagggctac
     7141 aacgttgagg tcgccaccga tggcgagacc ggcatcgctg ccgcgcagac caacccgcct
     7201 gacgcgatca tcctcgacgt gctcctgccc ggcatcgacg gctgggaggt cctgcgccgg
     7261 ctcaagggcg atcccgccct tcgcgacctg ccggtcgtgg tcgtcaccgt cgtcgacgag
     7321 cgaaacgtcg ccatgaacct gggcgcggtc gattacttcc tgaagccggt ccggcgtgag
     7381 gcgctgctgg ctcggctctc ccagtacacg ttcaccacca aggtcaaggt ggggcgcgtc
     7441 cgcgtgctgg cgatcgacga cgaccccgcg gcccgcgagc tggtcgcgaa tgcgctgcga
     7501 cccgagggct tcgacgtcgt ctccgcagca tcgggcaggg aagggctgga cctcgcgctc
     7561 gaggatccgc cggacctggt catctgtgac ctgctgatgc cggacatgga cggctacgag
     7621 gtcgtcgagc gcctccacgc caacgagagc accaaggacg tgacgatcct gattctcacc
     7681 ggccaggacc tgtcggctgc ggaccgcgag cggctgaacg ggaaggtggc cgaggtcgtg
     7741 cgcaaagggg tcgacccgcg ccccgccttc gcgcgatggc tgcggcgggc gtcggcagcc
     7801 gcccgcaggc gaagcgccga ggcgctgacg tcctaggacc cggaacgcct ctcagggttc
     7861 ggggccgaag tcccaggtat cggggatcac gctcggcggc ttgccgatga cgatggcgac
     7921 ctcgaggtgg gtcttcaact cgatgtccgc ggggttgccc tcgtacttct tgccatcgag
     7981 gtagacggcg acgctggtgt ccgacttgca gaactcgccg acgcactgat cgtcaagggg
     8041 gacgccccac tccgtaaaga actgcccgag cgtgtaaggc gcctggttgt ccttcttcga
     8101 ctcggtatgg atgatgccgc tcggatcgtg cgtgtgcagc ggagagaggc acggggcgaa
     8161 gcacagcgtc acgtagtact cggtcgcggt cccgtccgcg gtcttcttct ggtcgatgcc
     8221 ggcgggcgcc gcgatgtcga tgccgatgcc cgacgggacg accacgtcat tgccgtcgac
     8281 gaagacgtgg aggtgggcat gggtgtgcga gttgaggtgc tccgattgcg tcgcctcgag
     8341 ccccgccttc gtggtgagcg tcatgggatc ggccggagcc ggccatgacg gttgcttgag
     8401 gggctgaccg gaggtcgtgc aggccgccag catcaggccc gcaacgagga tggctgcgac
     8461 ggcccgatgc cgcccgacga ctgcccgcat tcccggcctc cggctagctc cgccttcgag
     8521 aagtcggagt ctacgctgcc aggctggcca ggagcgcctc gaggcgtgcg taggtctcgt
     8581 tggcgccgcg ctccgcgccg gaggcgacga tgctgtcccg ctgctcggtg gtgtcgaaga
     8641 gcgaggtgac cgtcagccgg gtgcgctcgc cgagatcccc gaaggtgatc gtctcgacgt
     8701 gggtcgtgcc catcccctcg accgagaagg tctgcgcgat cttctccggc ggggtgacct
     8761 cgcggtaggt gccgctgaag gctgcctcgt agccgttcgg gtagtgggcc acgaatcgcc
     8821 agctgccgcc ggtccgcacg tccatctcct cgacgacggt ctccgcgccc caccactggg
     8881 ggatcagggc tggatcggtg taggcagcga agacgcggtc tcgtggcgcg ttgaagaccc
     8941 gctcgatgcg gagttcgcgg tccgacggca tggtcagggt gacgggctgt gcctggggct
     9001 gtgcgctggt catgaggtcg ttcccttctt gcgttcgacg agttcaccga atcggtcgag
     9061 gcgctggcca accggttcag gcctgacctg atcacagggt aacggccgca ttccgacggg
     9121 cgtctcctgt gcagcctcgc ccaaccgggc agcgctcaat gcaggagaaa gctcgatgct
     9181 tcacagaatc cttccggcgc tagccgtcgt gacgatgctc gcccttcccg ccgccgccag
     9241 cgcggccacc ccggcgatct accacaccat cgatggcagg ttcgccagcg cctacttcac
     9301 cagcctggac ggctgcctcc agaccgatgt ctgggtctcg acctcggcgg ccacctatgc
     9361 tccccagcca ggaatccgtg acgcggtcaa caagcagggg cggacatcgg tcgagatctt
     9421 cgtgtacgac acctgccagc cgatggatgg caagcactac ccgatcgtgg cggactggtt
     9481 cgcgcaaggc tccgatcgtc tcgtcgccga gtcgcggatg cgctccgcgc acctcgcggc
     9541 gaacctcaag atgaccgatt ccgtctcagg cgattcagcg gtcgtgccga tcgatctgct
     9601 ctggcaggcg accgccgttg cgcaccccga caccgtgccg aacaaccacg tccgcttccc
     9661 gggcgagggg atcgtcaaca cgcacgacaa caacatcaac ctctccgccg tggcgtcggg
     9721 gacggtgctg ctgggcggtg tcaacgtctc ggccgacccc gacccaagcg ccatcctcga
     9781 gcagatcagg tcggcctgca tggagatcgg ctatccccac tgggacggcg acaccctgtt
     9841 ctgcttcggc ttctgacgcc cacccccgaa cggggtatcg tggcccgcga tggcgcgagc
     9901 gctgatcttc gacctcgacg gcacgctgat cgacaccgtc tacgcgcacg tctttgcctg
     9961 gcagcgggcc ctggcggagg caggcctgcc gatcgagggg tggcggatcc atcggcgcat
    10021 cgggatgagc ggcgggctct tcacccgtgc ggtggcacgc gagctcggcc gtccgctcag
    10081 cgacacggag gtcgaggaga tccagcggcg gcacggcgag ctgttccgcg agctcgtgcc
    10141 ggagcgccgt ccgctccccg gagcggtgga cctgctggcg cacctgcgcg aggccggcgt
    10201 gccgttcggc atcgcgacct ccggccgccg cccggagatc gacgcgtcgc tggaggtcct
    10261 gggcgtggga gccgacgtgg tcgtggtcga gcgcggcgac gtcctgcgcg ccaagcccga
    10321 gccggacctg ttcctcgcct gccaggagcg gatgggcgtc cacatccgcg acgtgacggt
    10381 cgtcggcgac gcggtctggg acctgctggc ggcccgccgt gcggggatgc tgtcagtggg
    10441 cctgctgacc ggtggctacg gcgaggacga gctggcgcgc gccggcgcgt tccgcgtgta
    10501 tcgcgacccg gcggagctga acgcctcgct cgatgagctg gggatcgtcc cgtagaggag
    10561 ctggcggtca cgcttcgtcg ctgggggccg ggcgatcgcg cgctggttga ccgcgtgatg
    10621 ggcgatccgc gcatgaccga gcacctcggc ggccccgagt cgccggagaa gctcgcggag
    10681 cgtcaggcac gatacgagcg gatccatgaa gcgggcacgg gcgggatgtt cgtgatctgc
    10741 gcgggtcccg aggccgaggg ggtggggtcg gtgggctact gggaggacgc ctggcgtggc
    10801 ggcacggtgt gggagatcgg ctggagcgtg gtcctcgagt tccaacgccg cggcattgcc
    10861 tcacgcgccg ttggcctgct gctcgaggag attgccgccg aagggctgga tcggccgatc
    10921 ctggcctacc ccgcggtgga gaacgaggcg tctaatgccg tctgccgcaa ggccggcatg
    10981 acgctcatcg gcgaggcggt ggtcgagttc cctcccggca atccgttccg cgcaaacgag
    11041 tggatccttc agccggcctc ggctcggagc tgatcgatcg ccgcaccgag atcggcggtg
    11101 gcctcttcca gcgtttcggc gggatgcatg ctgaaggcaa gccgcagcgc ccccggcgcg
    11161 gcgcgatcgt ccaggaagaa gcggtggccg ggcaggaatg ccatgccgcg ggccgcggct
    11221 acaggcagca gctcgtcggc cgtgattcct ggtgggagct caatccagac gaagtagccc
    11281 ccttcggggc gagaccatga cgcggtgtcg ggaaggtggg cgccgaggcc ggcgagtagc
    11341 cgatcgcgct gcgcccgata cgcgctacgg aagcgctcga cctgccgggc gtaatcgccg
    11401 ttggcggcgt attcggcaag caccgtggcg gcgaagtggt tcatcccccc gccggaatcg
    11461 agcaggccgc cggtcgccag gcgggtcgcg atctccgacg aggaggtcac gtagcccagg
    11521 cgcagccccg gcgccaccga cttcgcaaag ctgccgaggc ggatcaccga tcccgcgccg
    11581 tccagcgccc acagggatgg tggcggcggg ccgtcgtagg agagctcgcg ataggtgtcg
    11641 tcctcggcga tgagcaggct ctcgtccgcc gcgaaggcga ccagcgcgtt ccgtcgctcg
    11701 agcgagacct tgcgcccagt ggggttgtgg aaggtcggga tcgtgtacag gagtcgcggg
    11761 cgctctccgg cgcgtcggag gcgcgccacc gtgtcggcca ggtcatcgag cctgacgccg
    11821 tcggcgtccg aggccacggc ctcgagccgg accgggtgat cggcgaggat gctgatggca
    11881 aggtggtagg tcggcacgtc gacgattacg gtgtctcctg ccgaaaggag cagggttgcc
    11941 accaggtcga gcccccccga cgccccgctg gtgatcacca cctgggccgg gcttggggcg
    12001 cgtgcatcgg tttcggccag gcgctcgcag atgaagtcga tcaggggcgg cgatcccgcc
    12061 gtgctgccgt actccaggat gtccgagccg tggcggttca tggcacgtcg cgccgcgtcc
    12121 gccagctcac ggaccgggag caggctcggg tcagggtgtc cccaggcgag gtcgatgacg
    12181 cccggcgtgg cctcgtactg ggtgagcgag gaggcgatgc cgcgttcggt caccgggtca
    12241 ggggtccgtc gtgcaggctt cgtactcatt gccgggcaag agtgaccatc ggcaatgacc
    12301 cggtcaaccg tggcgagtac catccccgcc gacgtggata cgacgcgctc cccccttcag
    12361 gccgacctgg tggacatgct cgatgccctg cgcaatgcgg agcgggatct gttcgccatg
    12421 gtcccggccg accagcggga ggccgctggc acgatcggcg aatggtcgcc gaaggatctc
    12481 ctggcccacc tcggagcctg gcgtgccgtc gaggcgcggc ggttggaggc ccgagcgtcg
    12541 ggtggtgcgg ccagccccga cgaccccggc ctggatgagc cgatcgacga atcgaacgct
    12601 aggctccagg cgcgcgatcg ggacctgagc tgggggacgg tcgcggccga agcggatacg
    12661 agtatcgacg cgctcatcac ggccgttgac cggagctcaa ccgatgccct ctgcgagtgc
    12721 gacgacgcat cggtc
//
LOCUS       DATCQR010000021         3693 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_171530, whole genome shotgun sequence.
ACCESSION   DATCQR010000021 DATCQR010000000
VERSION     DATCQR010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3693
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171530"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            182..589
                     /locus_tag="VIN32_00820"
     CDS             182..589
                     /locus_tag="VIN32_00820"
                     /inference="COORDINATES: protein motif:HMM:NF018980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEY8921151.1"
                     /translation="MTDRTEPPPRKSAFALVRHLISGMVGLAKLEVQHGRQEIGAMLA
                     MARGGVVLIGIAAGLLVAALISLTVTLILGIAALLGWPGWVIALLVTLLLLLIAGLLA
                     WRGIRMIKIGPPEETIASVREEVAWAKRLLRRG"
     gene            559..1197
                     /locus_tag="VIN32_00825"
     CDS             559..1197
                     /locus_tag="VIN32_00825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921152.1"
                     /translation="MGETIAETRVEIAAQRAEMEADADRLRDALNLGRRIRENPGVVV
                     GIGAAAAFLVAGGPRRLARLLRRRLAPNTAEQAYDALPATMQTWVSTLAGEAGPRAAE
                     VRDQLVDELKRWRRDPIKDKKARKELAKRMVQGPPGPGRTAWSVAETALGLVAAALAR
                     KAIQRFLTDEPPAGDRAAAAAAAASATTSRDAAHNTADGPAPDDYAGWSKRS"
     gene            1240..1323
                     /locus_tag="VIN32_00830"
     tRNA            1240..1323
                     /locus_tag="VIN32_00830"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1273..1275,aa:Leu,seq:tag)
     gene            1344..2690
                     /gene="tig"
                     /locus_tag="VIN32_00835"
     CDS             1344..2690
                     /gene="tig"
                     /locus_tag="VIN32_00835"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEY8921153.1"
                     /translation="MVTVSSRPESGSRMVLEIEVSPTEVDRHFATAYRHVAERTEVPG
                     FRPGKAPRHVIDRFVGRASVIAEAVDHLVNESYDAALDQVSIVPIDQPEVDLDSGTVA
                     EGAPVKFTATVAVRPDVTLGSYTAYPFGLDPVEVTDEQVEAVITDLREQQGTLRPVDG
                     RGAAKGDIASVKFTGTIDGEPFEGGSADRLPLVIGEERMIPGWEENLVGMTIDQTKGF
                     EIDFPDDYRVDELQGKHAHFEATLLDLRERILPELDDEFAKSVGELSTVEELRAEIRD
                     ALARRGADEARHHFADRIIDFATANAGVELPEVMIANEIEIMRDELRNRLAAQQIGMD
                     RYLEMARQSPEELATELREPASRRVKSLLVLSAIAEKEGIDATPEQVEEEIADQLARY
                     PDEARLREYLTSRRGRSYLRMTLRNRTLVDTLIDRALGTDAVPAEASASDQDDTTE"
     gene            2695..3360
                     /locus_tag="VIN32_00840"
     CDS             2695..3360
                     /locus_tag="VIN32_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HEY8921154.1"
                     /translation="MLVPMVIESSARGERAFDIYSRLLKERIIFLGEPIEDHIANLVI
                     AQLLFLESEDPTKDISLYVNSPGGVVTSGLAIYDTMQYLKAPVSTICIGQAASMASVL
                     LAAGAKGKRFALPNSRIMIHQGSAGFRGNTPDVEIQMREVMHLTERLTGILAAHTGQT
                     PAKVKSDSERDFYMGADEAKAYGLIDEVFAGSTESLISMTQAAGGTVPTAKEAAAADK
                     AKK"
     gene            complement(3454..>3693)
                     /locus_tag="VIN32_00845"
     CDS             complement(3454..>3693)
                     /locus_tag="VIN32_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921155.1"
                     /translation="ARVPNRNVLAVRVANELEKHALLALDPTVFFTEPHYNGYPAVLV
                     RIPAIELDMLEKVLTDAWLTRASKQLAATLASRAI"
BASE COUNT          638 a         1255 c         1261 g          539 t
ORIGIN      
        1 cttcatcacc gagagcacgg cgggggtcca cgtctcgaac atcatgacca agctgggcgc
       61 taagagccgc tcggaggctg ccgccgtcgc ccatcgcatg ggcctcacct cgtacgacgg
      121 ggtccagcac tgacctgcca cgcttgaggg gaacgcgcgc cgtgccgtta gaatcggtcg
      181 cgtgacggac cgcaccgagc caccgccgcg caagagcgcc ttcgcgctcg tgcgtcacct
      241 gatctccggc atggtgggcc ttgccaagct cgaagtgcag cacggccgcc aggagatcgg
      301 ggcgatgctg gcgatggcgc gcggcggagt cgtgctcatc gggatcgccg ccgggctgct
      361 cgtcgcagcg ctcatctcgc ttaccgtcac cctcatcctg ggaatcgccg cgctcctggg
      421 ctggcccggc tgggtgatcg cgctccttgt tacgctcctg ctattgctca tcgccggttt
      481 gttggcctgg cgcggcattc gcatgatcaa gatcggaccg ccggaggaga cgatcgcatc
      541 ggtccgagag gaggtcgcat gggcgaaacg attgctgaga cgcgggtaga gatcgccgcc
      601 cagcgcgccg agatggaggc ggacgccgat cgcctgcgcg acgcgctcaa cctggggcgc
      661 cggattcggg agaaccccgg ggtggtggtg gggatcggtg ccgcggcagc cttcctggtc
      721 gccggcggac cccgacgcct ggcacgcctc ctgcgccggc gcctggcgcc gaacaccgcc
      781 gagcaggcat acgacgcgct gccggcaacc atgcagacct gggtcagcac gctggcgggc
      841 gaggccggac cgagggcagc cgaggtgcgt gatcagctgg tcgacgagct caagcgctgg
      901 cgacgcgacc cgatcaagga caagaaggcg cgcaaggagc ttgccaagcg gatggtccag
      961 ggacctcctg gccccgggcg gaccgcctgg agcgtcgccg agacggcgct gggcctggtc
     1021 gccgcggcac tcgcccgcaa ggccatccag cgattcctga ccgatgagcc gcccgccgga
     1081 gaccgtgccg cggcagctgc tgcagccgcg tccgccacca cctcccgtga cgccgcccac
     1141 aacacggctg acggccccgc ccccgacgac tacgccggct ggtcgaagcg gtcctgacgt
     1201 agactccgtc tgaggatatc gcgccgtcca cgcatccgcg ggcgagtggc ggaacggcag
     1261 acgcgccggc tttaggagcc ggtgtcgcaa gacgtgcgag ttcgaatctc gcctcgccca
     1321 ccacgtcagc agcaggagca gccttggtca ccgtctcttc ccgccccgag tccggatcga
     1381 ggatggtcct cgagatcgag gtctcgccga ccgaggtgga ccgccacttc gcgaccgcct
     1441 accgccacgt cgccgagcgc accgaggtgc caggcttccg gccaggcaag gcgccgcgcc
     1501 acgtgatcga tcggttcgtt ggacgagcct cggtgatcgc cgaggcggtc gaccacctcg
     1561 tcaacgagag ctatgacgcg gcactggacc aggtctccat cgtcccgatc gaccagcccg
     1621 aggtggacct cgacagcggc accgtggccg agggtgcgcc cgtcaagttc accgccaccg
     1681 tggctgtccg tccggatgtg acgctgggct catacacggc ctacccgttc gggctggatc
     1741 cggtcgaggt gaccgatgag caggtcgagg ccgtcatcac cgacctgcgc gagcagcagg
     1801 gcacgctgcg accggtcgac ggtcgaggcg cggccaaggg cgatatcgcc tcggtcaagt
     1861 tcacaggcac catcgacggc gagccgttcg agggcggatc ggccgaccgg ctgccgctcg
     1921 tgatcggcga ggagcggatg atccccggct gggaggagaa tctcgtcggc atgaccatcg
     1981 accagaccaa gggattcgag atcgacttcc ccgatgacta ccgggtcgat gagctccagg
     2041 gaaagcacgc gcatttcgag gcgacgctgc tcgacctgcg cgagcgaatc ctgcccgagc
     2101 tggacgacga gttcgcgaag agcgtaggcg agctctcgac cgtcgaagag ctgcgcgccg
     2161 agatccgcga cgcgctggcg cggcgggggg ccgatgaggc gcgccaccac ttcgccgacc
     2221 gcatcatcga cttcgccacc gccaacgccg gcgtcgagct gcccgaggtg atgatcgcca
     2281 acgagatcga gatcatgcgc gatgagctgc gcaaccggct ggccgcccag cagatcggca
     2341 tggacaggta cctggagatg gctcgccagt cacccgagga gctggcgacc gagctgcgcg
     2401 agcctgccag ccggcgcgtg aagtcactgc tcgtgctatc cgccatcgca gagaaggagg
     2461 ggatcgacgc gacccctgag caggtagagg aggagatcgc agaccagctt gctcgctacc
     2521 ccgatgaggc gcgcctgcgc gagtacctca cctcgcgccg tggccgctcg tacctgcgca
     2581 tgacgctgcg caaccgtaca cttgtcgaca cgctgatcga ccgcgcgctg ggcacggacg
     2641 cagtcccggc cgaggcatcg gcctccgacc aagacgacac cacggagtag acgcatgctg
     2701 gtcccgatgg tcatcgagtc gagcgctcgg ggtgagcggg ccttcgacat ctactcgcgg
     2761 ttgctcaagg agcggatcat cttcctgggc gagccgatcg aggatcacat cgcgaacctg
     2821 gtcatcgccc agctcctctt cctggagagc gaggatccga ccaaggacat cagcctgtac
     2881 gtcaactcgc cgggcggcgt ggtcaccagc ggcctcgcca tctacgacac gatgcagtac
     2941 ctcaaggccc cggtcagcac catctgcatc gggcaggcgg cgagcatggc gtcggtgctc
     3001 ctggcggccg gtgccaaggg gaagcgcttc gccctgccga acagccggat catgattcac
     3061 cagggctcgg ccgggttccg gggcaacacc ccggacgtcg agatccagat gcgcgaggtg
     3121 atgcacctca ccgagcggct cactggaatc ctggccgcgc acaccgggca gacgcccgcg
     3181 aaggtgaaga gcgactcgga gcgtgacttc tacatgggcg ccgatgaggc gaaggcctac
     3241 ggcctgatcg acgaggtctt cgccggttcc accgagtcac tgatcagcat gacccaggcc
     3301 gcgggtggca ccgtgccgac cgccaaggaa gctgccgcag cggacaaagc caagaagtag
     3361 ttgatggccg gcccacaccc ggccttgcac ggcttcgcta cgcgcttcgg ccggccgctg
     3421 cccgtcccca acttcacgta ttcgctagct gcatcagatc gcgcgggagg cgagggttgc
     3481 ggcgagctgc ttggatgccc gggtcagcca ggcgtcggtg agaaccttct ccagcatgtc
     3541 cagctcgatg gcggggatcc gaacgagcac cgcggggtag ccgttgtagt gcggctcggt
     3601 gaagaagacg gtgggatcca gcgcgaggag cgcatgcttc tccagctcat tggccacccg
     3661 cacggcaagg acgttgcgat tggggacccg tgc
//
LOCUS       DATCQR010000022         2572 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_47709, whole genome shotgun sequence.
ACCESSION   DATCQR010000022 DATCQR010000000
VERSION     DATCQR010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47709"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1010)
                     /locus_tag="VIN32_00850"
     CDS             complement(<1..1010)
                     /locus_tag="VIN32_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEY8921156.1"
                     /translation="MPSDRIVVRGAREHNLKNLDVEMPRDRLIVITGLSGSGKSSLAF
                     DTIYAEGQRRYVESLSAYARQFLGQMEKPDVDQIDGLSPAISIDQKGSSRNPRSTVGT
                     VTEVYDYLRLLFARIGRMHCPICGREIARQTVEQIIDQLYELPDGTRLMLLAPLVRDR
                     KGEHEKLLAGAKQGGFVRVRVDGEFRDLDEEIDLDKKFKHSIEIVVDRLVVRRTDDDG
                     TPRPDAARMADSIETALRLSDGTLMVHLPDEGPKGSDRLYSERYACPEHGGSFEEPAP
                     RNFSFNSPHGACPACTGLGNRLEIDPDLVLPNPSLSLAEGAVLPWRRMVATASWFATI
                     LEA"
     gene            complement(1056..1583)
                     /locus_tag="VIN32_00855"
     CDS             complement(1056..1583)
                     /locus_tag="VIN32_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921157.1"
                     /translation="MNRETRIGVGLVAALGALVFVMLVVGSLGEPRPELTEYPVGEIL
                     AQQDRAAQHHATVLRIVGWYAELAGDCVGDHGGVDASVAWLQRDCPLRVLLSQQPSED
                     VTQAELERDGLRLAAPDGRPFPSRAQPGGANLRLQQLIFTGHFDDPAATKCVPDRADR
                     CRNTFVVSTYDGLLR"
     gene            complement(1580..2140)
                     /locus_tag="VIN32_00860"
     CDS             complement(1580..2140)
                     /locus_tag="VIN32_00860"
                     /inference="COORDINATES: protein motif:HMM:NF015146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEY8921158.1"
                     /translation="MPLCDGGSRPVLSGDGSEPAMLIGQAPGWREIETGLPFAHDAGK
                     RLAGWLALAGISVDDFRSRWYVTSVGKCYPGRRPGSSVDSPPSRGEVLRWSPYLESEV
                     RLVAPRLIALVGGLAHRHAFGASARLDDLVGKELAWDAAPGASVIALPHPSGASTWLN
                     DPARIELWRRGIELLAARWQALREAS"
     gene            complement(2245..>2572)
                     /locus_tag="VIN32_00865"
     CDS             complement(2245..>2572)
                     /locus_tag="VIN32_00865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921159.1"
                     /translation="FERYLHWLELARELLADRLDAAGDAAPRDALERIAKQDLLLTAQ
                     LGGSPTEIGGELVDRLYQARDGLTAALEGAGAMGDGVRRAVRLAIADDLRLADHLADQ
                     LAGAAS"
BASE COUNT          389 a          913 c          889 g          381 t
ORIGIN      
        1 gcctccagga tcgtggcgaa ccacgatgcc gtcgcgacca tgcgccgcca cggcaggacc
       61 gcgccctccg ccagtgagag cgacggattg ggcaggacca ggtcgggatc gatctcgagc
      121 cggttgccga gccccgtgca ggccgggcag gcaccatgcg gcgagttgaa gctgaagttg
      181 cggggcgccg gctcctcgaa gctgcccccg tgctcgggac aggcataccg ctcgctgtag
      241 agccggtcgc ttcccttcgg cccctcgtcg gggaggtgga ccatgagtgt cccgtccgag
      301 agccggaggg cggtctcgat cgagtcggcc attcgggcgg catcgggccg gggagtgccg
      361 tcgtcgtcgg tccgacgaac caccaggcgg tcgacaacga tctcgatcga gtgcttgaac
      421 ttcttgtcga ggtcgatctc ctcgtccagg tcgcgaaact cgccgtccac ccggacccgg
      481 acaaaaccac cctgcttcgc gccggcgagg agcttctcgt gctcgccctt gcggtcgcgg
      541 accagcggtg ccagcagcat cagccgcgtc ccgtcgggga gctcgtagag ctggtcgatg
      601 atctgctcca ccgtctggcg ggcgatctcg cggccgcaga tcgggcagtg catgcgaccg
      661 atccgcgcga agaggagtcg caggtagtcg tacacctcgg tcacggtgcc gactgtcgag
      721 cgcgggttcc ggctggagcc cttctggtcg atgctgatcg cggggctgag cccgtcgatc
      781 tggtcgacgt cgggcttctc catctgcccc aggaactggc gcgcgtaggc gctcagcgac
      841 tcgacgtagc gtcgctgtcc ctcggcgtag atggtgtcga aggcaaggct gctcttgcca
      901 ctgcccgaca gcccggtgat cacgatcaac cggtcgcgcg gcatctccac gtcgaggttc
      961 ttgaggttgt gttcgcgcgc gccacgcacg acgatgcggt cggaaggcat tgggtcgatg
     1021 atatcagacc gatgtgaacg cctgttcggt tcagcctacc gcagcaggcc gtcgtaggtg
     1081 ctgacgacga acgtattgcg gcagcggtcc gcgcgatccg ggacgcattt cgtcgcggct
     1141 gggtcgtcga aatggccggt gaagatgagc tgctggagcc gcaggttcgc tcctcccggc
     1201 tgcgccctgg atgggaaggg ccggccatcg ggagccgcca gccgcaggcc gtcccgctcc
     1261 agctcggcct gggtcacgtc ctcgctcggc tgctggctga gaagcacccg cagggggcaa
     1321 tcgcgctgga gccaggcgac ggacgcgtcc actcccccgt gatcgccgac gcagtcaccg
     1381 gccagctcgg cgtaccagcc cacgatccgc agcacggtag cgtgatgctg tgcggcccga
     1441 tcctgctgcg ccaggatctc gccgaccggg tactccgtca gctccggccg cggctcaccg
     1501 agcgagccga ccaccagcat cacgaacaca agcgcgccga gggcggcgac cagcccgacg
     1561 ccgatccggg tctcccggtt cacgatgcct cgcggagcgc ctgccagcga gcggccagca
     1621 gctcgatccc ccgtcgccag agctcgatac gcgccgggtc attcagccag gttgacgccc
     1681 ccgacgggtg cggcagcgca atgaccgatg cgcccggcgc cgcgtcccag gcgagctctt
     1741 tcccgaccag gtcgtccagc cgggcgctcg caccgaatgc atggcgatgc gccaggccac
     1801 cgaccagcgc gatcaaccgc ggggcgacga ggcgcacttc gctttcgagg tagggcgacc
     1861 agcgcagcac ctcgccgcgt gagggcggcg aatcgaccga tgaacccggc cgccgccccg
     1921 ggtagcactt cccgaccgag gtcacgtacc agcgcgagcg aaagtcgtcg acgctgatcc
     1981 cggccagcgc cagccacccg gccagtcgct tgccggcgtc atgcgcgaac ggcaggcccg
     2041 tctcgatctc gcgccacccg ggggcctgcc cgatcagcat cgccggctcg ctcccatcgc
     2101 ccgaaaggac cggccgcgag ccgccgtcgc agagtggcat ggcatcctgc agggcgcgca
     2161 gatcagtgcc cggcggggcg cggcggggct cggggatggg ccagtcgcca gcgtggcgga
     2221 cccgcttcgg gccggatggt gcgatcagct ggcagctcct gcgagctgat cggcgaggtg
     2281 atcggcgaga cggaggtcat cggcgatcgc cagccgaacc gcacgccgca cgccgtcgcc
     2341 catggcgccc gcaccctcca gcgccgcggt cagtccgtcc cgtgcctggt acaggcggtc
     2401 gaccagctca ccgccaatct ccgtcggcga gccgccgagc tgggccgtca ggaggaggtc
     2461 ctgcttggct atccgctcga gcgcatccct tggtgccgcg tcgccggccg catcgagacg
     2521 atccgcgagg agctcgcggg ccagctccag ccagtgcagg tagcgctcga ac
//
LOCUS       DATCQR010000023         2642 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_16682, whole genome shotgun sequence.
ACCESSION   DATCQR010000023 DATCQR010000000
VERSION     DATCQR010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2642
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16682"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..397
                     /locus_tag="VIN32_00870"
     CDS             <1..397
                     /locus_tag="VIN32_00870"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020374954.1"
                     /note="catalyzes the radical-mediated insertion of two
                     sulfur atoms into an acyl carrier protein (ACP) bound to
                     an octanoyl group to produce a lipoyl group; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEY8921160.1"
                     /translation="TETVPRLYPSIRPKAVYANSLGLLRHVKALRPEMVTKSGVMVGL
                     GESSDELLQVFRNMREHDIDVLTVGQYLRPTKKHAEVQRFYRPEEFATLREQALAMGF
                     RHVESGALVRSSYHADEQVPTPGQKLLAG"
     gene            354..1043
                     /gene="lipB"
                     /locus_tag="VIN32_00875"
     CDS             354..1043
                     /gene="lipB"
                     /locus_tag="VIN32_00875"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010925.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEY8921161.1"
                     /translation="MNRSRPRVRSCWLGSVPYRQAWDLQRSLVEQVRAGEAPDTLLLL
                     EHPHVYTMGRKGTAEHLLWDEAERERRAVDLVWSDRGGDATYHGPGQLVGYPIFDLAR
                     HGLDLLVYLRTLEASLIDYLAEQGLVGAAVPGLTGVWVGDAKVAAIGVKFNGGVVSHG
                     FALNLTTDLEYFEGIVPCGIADKKATSVEHLKGSRLSTETAARAYADRFARAFGVELD
                     WTARLPELVGA"
     gene            1178..1465
                     /locus_tag="VIN32_00880"
     CDS             1178..1465
                     /locus_tag="VIN32_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921162.1"
                     /translation="MSSTTELIPLATIVPPANHEVGGGFDAVVDGEAVRVRAVLERTV
                     VVEHPSTGDRSVVRKDLCMVDPGAVMFVPGNPSAGVGPRQWRRLPGKPPQP"
     gene            1553..1918
                     /locus_tag="VIN32_00885"
     CDS             1553..1918
                     /locus_tag="VIN32_00885"
                     /inference="COORDINATES: protein motif:HMM:NF039183.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5658 family protein"
                     /protein_id="HEY8921163.1"
                     /translation="MHAHLRSPSSRVLAIWILLLAGAQVADVITTGVAMAHGGVEANR
                     LVAALLSHGGLWLVFGLKQLLVAAMAIACLVLKRYAEAHPSFQARAAHSFVWRAIQLS
                     VIGLVLVAVHNTALLAQIA"
     gene            complement(1932..2135)
                     /locus_tag="VIN32_00890"
     CDS             complement(1932..2135)
                     /locus_tag="VIN32_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921164.1"
                     /translation="MARRFVSRFVVVVGGMGALGYILGYAARVPELRGLSTDQQAWAV
                     FALGLIAAILLLFRAAQVGEVSP"
     gene            complement(2335..2469)
                     /locus_tag="VIN32_00895"
     CDS             complement(2335..2469)
                     /locus_tag="VIN32_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921165.1"
                     /translation="MVLVLLLELLDKFRGAWKALLVGFDRQRAAAGSQPESRSNRRAF
                     "
BASE COUNT          425 a          920 c          861 g          436 t
ORIGIN      
        1 caccgagacc gtcccccggc tctacccgag catacggccc aaggcggtct acgcgaacag
       61 cctcgggctg ctgcgtcatg tcaaagcgct gcggccggag atggtcacca agtcgggcgt
      121 catggtcggc ctcggcgagt cgtccgacga gctgctccag gtcttccgca atatgcgcga
      181 gcatgacatc gacgtcctta ccgtcggcca gtacctgcgg ccgaccaaga agcacgccga
      241 ggtccaacgc ttctaccgtc ccgaggagtt cgcgacattg cgcgagcagg ccctcgcgat
      301 gggttttcgc cacgtcgagt cgggcgccct cgtccgctcc tcctaccacg ccgatgaaca
      361 ggtcccgacc ccgggtcaga agctgctggc tgggtagcgt cccctatcgc caggcgtggg
      421 acctgcagcg gtccctcgtc gagcaggtgc gcgccggcga ggcgcctgac accctcctgt
      481 tgctcgagca tccccacgtc tacacgatgg gtcggaaggg aacggccgag cacctgctct
      541 gggacgaggc ggagcgcgag cggcgcgcgg tcgacctcgt ctggtccgat cggggcggtg
      601 acgcgaccta tcacgggccc ggccagctgg tgggctatcc gatcttcgac ctcgcccggc
      661 acggcctcga cctcctcgtc tacctgcgaa cgctggaggc gtcgctgatc gactacctgg
      721 cggagcaggg actggtggga gccgccgtgc ccggcctcac cggtgtctgg gtcggcgatg
      781 ccaaggtggc cgcgatcggc gtcaagttca acgggggcgt ggtcagccac ggctttgcgc
      841 tcaacctgac caccgacctc gagtacttcg agggcatcgt cccctgcggt atcgccgaca
      901 agaaggcgac ctccgtcgag catctcaagg ggagccgcct gtcgaccgag acagccgctc
      961 gtgcctatgc cgatcgtttc gcccgcgcct tcggcgtcga gctcgactgg acagcgcggc
     1021 taccggagct ggtcggagcc tgaaaccccg ggccggtcgg cccgcatcga acggatgctc
     1081 gcgccggatt tggtagaatt ctccagtttc gtgctcccca gccggtcgtc cccgcgcgtg
     1141 cgcaagcagg ccggtgcaat ttcgaggtgt ccactacatg agcagcacta ctgaactgat
     1201 ccccctggcg accatcgtgc cgcccgccaa ccacgaggtc ggcggcggtt tcgacgcggt
     1261 cgtcgatggc gaagcggtgc gggtccgcgc ggtcctcgag cggacggtcg tggtggagca
     1321 cccgagcacg ggggatcgca gcgtggtgcg caaggacctc tgcatggtcg acccgggagc
     1381 cgtcatgttc gtgccgggca acccgagcgc cggggtggga ccgcgccagt ggcgtcgcct
     1441 gcccggcaag ccgccccagc cctaacccgg caccggccgg caaccggccg gtttgttttc
     1501 cacttggatg ctctggcgtt tgcggtagtg tcgctatcat cttttctagt agatgcacgc
     1561 tcatctccgc tccccttcca gccgtgtcct cgccatctgg atcctgctcc tggccggtgc
     1621 gcaggtggcc gatgtcatca ccacgggggt cgccatggca cacggaggag tggaagccaa
     1681 tcgtctggtt gccgcgctgc tcagccacgg cggtttgtgg ctcgtcttcg gactgaagca
     1741 gctgctcgtc gccgcgatgg cgatcgcctg cctggtcctg aagcggtacg ccgaggctca
     1801 cccttccttc caggcccgcg ccgcccacag cttcgtctgg cgcgcgatcc aactctcggt
     1861 gatcggcctg gtgctggtcg ccgtgcacaa cacggcactg ctcgcgcaga tcgcctagga
     1921 cgtgccgcgc ctcatgggct gacctcgccg acctgggccg cccggaagag aagcaggatg
     1981 gcggcgatca ggccgagggc gaagaccgcc caagcctgct gatcggtgct gaggccgcgg
     2041 agctccggca cgcgcgccgc gtaacccagg atgtagccga gcgcgcccat cccgccgaca
     2101 accacgacaa accggctgac aaatcgccgc gccatcatcg cgccgatcag gttcgccgag
     2161 aaggctgaag cgatcgttaa gcgaacctga agggcggtcc ggtcggtcct ggaaatgaga
     2221 aagccccgga gggtgaccgg ggcttctctg cggaggagag gtaactccca tcctcgcgcg
     2281 caaacgtgaa gccagcctaa agaggcgttt tcggtgacca gagagccgct ctgctcagaa
     2341 ggctctgcgg ttggagcggg actccggttg ggagcccgcc gccgcccgct gccgatcgaa
     2401 gccgaccaac agcgccttcc aggcgccgcg gaacttatcg agcagctcga gcagcaatac
     2461 caataccact atcacctagt ctaacgcaag caggcgaacg aatcctgcgg ctgccactcc
     2521 cgggcaagcc cttgacgcgc ctctccgagc gggttaggct cccgcaaccg tgctgcgaac
     2581 agccatcgtc ctcctcgcgc tcctgctgag cacggcctgc ggcggctcgt cgccgtcgag
     2641 cc
//
LOCUS       DATCQR010000024         2466 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_218131, whole genome shotgun sequence.
ACCESSION   DATCQR010000024 DATCQR010000000
VERSION     DATCQR010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218131"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2420)
                     /gene="uvrA"
                     /locus_tag="VIN32_00900"
     CDS             complement(<1..2420)
                     /gene="uvrA"
                     /locus_tag="VIN32_00900"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEY8921166.1"
                     /translation="MPSDRIVVRGAREHNLKNLDVEMPRDRLIVITGLSGSGKSSLAF
                     DTIYAEGQRRYVESLSAYARQFLGQMEKPDVDQIDGLSPAISIDQKGSSRNPRSTVGT
                     VTEVYDYLRLLFARIGRMHCPVCGREIARQTVEQIIDQLYELPDGTRLMLLAPLVRDR
                     KGEHEKLLAGAKQGGFVRVRVDGEFRDLDEEIDLDKKFKDSIEIVVDRLVVRRTDDDG
                     TPRPDAARMADSIETALRLSDGTLMVHLPDEGPKGSDRLYSERYACPEHGGSFEEPAP
                     RNFSFNSPHGACPACTGLGNRLEIDPDLVLPNASLSLAEGAVLPWRRMVATASWFATI
                     LEAVADRYGFGTDTPIGELSEEAREILLRGNHGERVTVRYKSRQGQEHSYSTTFEGVL
                     PNLERRYRETSSEATKTEIERYMTQRPCPTCGGMRLKPESISVTVGDRNIIETTRLAV
                     TDALAWYAGLPDLLTERENQIARQVLKEIRARLGFLVDVGLDYLTLDRASGSLSGGEA
                     QRIRLATQIGSSLMGVLYILDEPSIGLHQRDNDRLIATLMRLRDLGNTLIVVEHDEET
                     IRAADWVIDIGPGAGEHGGELIHSGSLPELLHSKRSITAAYLRGDRSVPVPAQRRRGS
                     GEWLTVRGARENNLRNVDIAFPLGRFVAVTGVSGSGKSSLVTQILYPALAARIWGARE
                     RPGAHDRIEGIDRVDKVIEIDQSPIGRTPRSNPATYTGLWAPLRELLAAVPESRLRGY
                     RPGRFSFNVKGGRCEACEGAGIVQIEMHFLPDVYVQCEVCKGKRYNRETLEIHYKGRN
                     VAEILEMTV"
BASE COUNT          361 a          857 c          823 g          425 t
ORIGIN      
        1 accgtcatct ccaggatctc ggcgacattg cgccccttgt agtggatctc gagtgtctcg
       61 cggttgtaac gcttcccctt gcagacctcg cactggacgt agacgtcggg caggaagtgc
      121 atctcgatct gcacgatccc agctccctcg caggcctcgc agcgcccgcc cttgacgttg
      181 aagctgaaac gtccgggccg atacccccgc agccgcgact ccggcacggc ggcgagcagc
      241 tcgcgcagcg gcgcccacag cccggtgtag gtggcggggt tcgaacgcgg cgttcgcccg
      301 atcgggctct ggtcgatctc gatcaccttg tcgacgcggt cgatcccctc gatcctgtcg
      361 tgagctccgg gacgctcgcg ggcgccccag attcgcgccg ccagggccgg gtacaggatc
      421 tgggtcacca ggctcgactt gcctgatccg ctcaccccgg tcacggccac gaaccggccc
      481 agcgggaagg cgatatcgac gttgcgcagg ttgttctcgc gcgcgccgcg aaccgtcagc
      541 cactccccac ttccgcgccg gcgctgcgcc ggcacgggga cagagcggtc gccgcgcagg
      601 tatgccgcag tgatcgagcg tttgctatgg agcagctcgg gcaacgatcc cgagtggatc
      661 agctcgccgc cgtgctcgcc cgccccgggg ccgatgtcga tcacccagtc ggcggcgcgg
      721 atcgtctcct cgtcgtgctc gaccacgatc agggtgttgc ccaggtcgcg cagccgcatc
      781 agggtcgcga tcagccggtc gttgtcccgc tggtgcaggc cgatcgacgg ctcgtcgagg
      841 atgtacagga cccccatcag gctcgagccg atctgcgtcg ccagccggat ccgctgtgcc
      901 tcgccaccgg agagcgagcc gcttgcccgg tcgagggtca ggtagtcgag ccccacgtct
      961 accaggaagc cgagccgcgc acggatctcc ttgagcacct gccgagcgat ctggttctcg
     1021 cgctcggtga gcaggtccgg cagcccggcg taccaggcca gcgcatcggt caccgccagc
     1081 cgggtcgtct cgatgatgtt ccgatcgccg accgtcacgg agatggattc gggcttgagc
     1141 cgcatccctc cacaggtcgg gcacggccgc tgcgtcatgt aacgctcgat ctcggtcttg
     1201 gtcgcctcgg agctggtctc gcggtagcgg cgctcgaggt tgggcaggac cccctcgaag
     1261 gtcgtcgagt acgagtgctc ctgcccctgc cgcgacttgt agcggaccgt gactcgctcg
     1321 ccgtggttgc cgcggagcag gatctcgcgc gcctcctcgg agagctcgcc gatcggcgta
     1381 tcggtcccga agccataccg gtccgcgaca gcctccagga tcgtggcgaa ccacgatgcc
     1441 gtcgcgacca tgcgccgcca cggcaggacc gcgccctccg ccagcgatag cgacgcattg
     1501 ggcaggacca ggtcgggatc gatctccagc cggttgccga ggcccgtgca ggccgggcag
     1561 gcaccatgcg gcgagttgaa gctgaagttg cgcggcgccg gctcctcgaa gctgcccccg
     1621 tgctcgggac aggcataccg ctcgctgtag agccggtcgc tccccttcgg cccctcgtcg
     1681 gggaggtgga ccatgagcgt cccgtccgag aggcggaggg cggtttcgat cgagtcggcc
     1741 attcgggcgg catcgggccg gggagtgccg tcgtcatcgg tccgacgcac caccaggcgg
     1801 tcgacaacga tctcgatcga gtccttgaac ttcttgtcga ggtcgatctc ctcgtccagg
     1861 tcgcgaaact cgccgtccac ccggacccgg acaaagccac cctgcttcgc gccggccagg
     1921 agcttctcgt gctcgccctt gcggtcgcgg accagcggtg ccagcagcat cagccgcgtc
     1981 ccgtcgggga gctcgtagag ctggtcgatg atctgctcca ccgtctggcg ggcgatctcg
     2041 cggccgcaga ccgggcagtg catgcgaccg atccgcgcga agaggaggcg caggtagtcg
     2101 tacacctcgg tcacggtccc gacggtcgag cgcgggttcc ggctcgagcc cttctggtcg
     2161 atgctgatcg cggggctcag cccgtcgatc tggtcgacgt ccggcttctc catctgcccc
     2221 aggaactggc gtgcgtaggc gctcagcgac tcgacgtagc gtcgctgtcc ctcggcgtag
     2281 atggtgtcga aggcaaggct gctcttgcca ctgcccgaca gcccggtgat cacgatcaac
     2341 cggtcgcggg gcatctccac gtcgaggttc ttgaggttgt gttcgcgcgc gccacgcacg
     2401 acgatgcggt cggaaggcat tgggtcgatg atatcagacc gatgtgaacg cctgttcggt
     2461 tcagcc
//
LOCUS       DATCQR010000025         4514 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_94193, whole genome shotgun sequence.
ACCESSION   DATCQR010000025 DATCQR010000000
VERSION     DATCQR010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4514
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94193"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="VIN32_00905"
     CDS             <1..369
                     /locus_tag="VIN32_00905"
                     /inference="COORDINATES: protein motif:HMM:NF014805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEY8921167.1"
                     /translation="VRGILNVQMIVADQRIVVIEANPRASRTVPIVAKATGHDIVAAA
                     VRCALGATLAQVGWQPGLAADAPLVAVKTPIGSLWRLPGVDATLGPEMRSTGEVLGLA
                     PDYAGAMAAAEAAALAHAPR"
     gene            complement(377..1045)
                     /locus_tag="VIN32_00910"
     CDS             complement(377..1045)
                     /locus_tag="VIN32_00910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921168.1"
                     /translation="MPKKSSAQPDAERRAEARRRARLQAQGRLEEEWDEQDEVEAAPA
                     RRSSPSMVQRLFPPALPLPGKPDPLAGFTYSGRLRPVVSSLWLIPRNLFAGVVMGILW
                     AAAYIVTIQYAQSMVGSIASFASFGALVAAGWIGWQRPWLYGFVAAVIGYILYTIYVI
                     IALAGNPALNQPGATTQAALFLLTNGVLQAGIGALAGFYGGYLRRRMADPAGRQRTAA
                     RRRR"
     gene            1151..3700
                     /locus_tag="VIN32_00915"
     CDS             1151..3700
                     /locus_tag="VIN32_00915"
                     /inference="COORDINATES: protein motif:HMM:NF013125.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HEY8921169.1"
                     /translation="MSVVRAVPFARHRLLAGLISIGVLAIAIDHAVAFPLAQGGGATT
                     APLVEATGHLVVSEVMTGGASASDEFVELYNPSSTMLPLEGLELVYVTASGATVTRKA
                     SWAAGAPGIPRGAHLLLANSAGIFAGLADVAYANGLAATGGSVALRVIGAATAIDAVG
                     WGSATSTWLETRPAPAPAAGSSLERLPGASLGSGQDTNDNLIDFVIQSAPDPQNSGSP
                     PIPVPSDSPVQTGSPAPSGSAEPSASQTTEPSVEPSLTGTPAASATATPSATVTPRPT
                     ATPAPTATPTPTPSPTAAPLSIGDARSQADGTTVTVEGVTLTASDFTDGGGYLVDATG
                     GIAVLVSDGTFGRGQRLRVTGTIDDRYAQRTIRATADAITNMGVGSEPLAADTPTGSI
                     GEGTEGRLVEVSGLIISSVTTLSTGLAWDLDDGSGAIRILVGTATAIDTSTWVRGVGL
                     RLIGVVGQRDSSGTGTSGFRIQPRETADLLTVEPIATPSPTPTPQPTATPTPSGSPSP
                     SATPTPGSRLVSIADARAATAGTHLRIRGVVTAPSGLIDTGSAVVQDATAGILVRLGT
                     DVGSLSLGQLVELDGTRATKTGMLSLRVATPPVQLGTQAEPEPMRRATGALGESQEAR
                     LVIVRGSISTAISRPRGGNVSFAIDDGSGPIRVTISSRSGISDGSLTRGAWLELRAVL
                     AQETTGSAPSSGYRLWPRVASDLRVIAAPVAGSTQSAPCCASVQTNDYPLPDSAVADA
                     LLGQAAGPPGQEVTPVLARPQPTSSPRPTSSTGQEAKAASDQRAPAGGLVVAGMGLGT
                     LAGLLAWFGRRRAPDDDDEPGSIDAEGGPRLSVLRAEGNDARNERGILPPT"
     gene            3736..>4514
                     /locus_tag="VIN32_00920"
     CDS             3736..>4514
                     /locus_tag="VIN32_00920"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HEY8921170.1"
                     /translation="MTDLSQADAPRTGGRQYHIALERGELAEYIVLVGDPGRVAKVAS
                     LWDSVELNRSNREITTATGTYQGMRISAMSTGMGTDNVEIVLAEVMEITDQPTLIRVG
                     SSGALQPEIALGDLIVSIGAVRLENTTDFYVHPGYPAIAHRDVVWALEAACLETGARY
                     HVGMTATASGFYAPQGRAMRTLPVRYPDLAEELRRQRVANLEMESSALFVLAGLAGLR
                     AGTVCAAYAQRVDGTFLEGAAKEAAEARCIDVGLAGIHLLWV"
BASE COUNT          695 a         1569 c         1577 g          673 t
ORIGIN      
        1 gtgcggggaa tcctgaacgt ccagatgatc gttgccgatc agcggatcgt cgtgatcgaa
       61 gccaatcctc gtgcgagccg gacggtgccg atcgtggcca aggcgaccgg acatgacatt
      121 gttgccgccg cggtgcgatg cgcgctgggt gcgacgctgg cgcaggtcgg ctggcagccc
      181 gggctggccg cggatgcgcc actcgtggcc gtcaagacgc cgatcggttc actctggcgg
      241 ctgccgggtg tggacgcgac cctcggtccc gagatgcgct ccaccggcga ggtgctgggg
      301 ctggcccctg actacgccgg ggcgatggcg gccgccgaag cggctgcgtt ggcgcacgcc
      361 ccgcgctgag attccgtcag cggcggcggc gcgcggcggt ccgctggcgt cccgcggggt
      421 ccgccatgcg acggcgcagg tagcccccgt agaagcctgc cagggcgccg atcccggcct
      481 gcagcacacc attcgtgagg aggaagaggg ccgcctgggt ggtcgcgccg ggctggttga
      541 gcgccggatt cccggcgagc gcgatgatca cgtagatcgt gtagaggatg tacccgatca
      601 ccgccgccac gaagccgtat agccacggcc gctgccagcc gatccagccg gcggccacca
      661 gtgctccgaa cgaggcgaat gacgcgatgc tgcccaccat ggactgcgcg tactggatgg
      721 tgacgatgta ggccgccgcc cagaggatgc ccatcacaac gccagcgaac aggttgcggg
      781 ggatcagcca cagactcgaa acgacggggc gcagtctccc cgagtaggta aagccggcca
      841 gaggatccgg cttgccgggg aggggtagcg ccggtggaaa gagccgttgg accatcgagg
      901 gagacgaccg cctcgcgggg gccgcctcga cctcgtcctg ctcgtcccac tcctcctcga
      961 ggcgtccctg cgcctggagg cgggctcggc gacgtgcttc tgcgcgtcgt tcagcgtccg
     1021 gctgggcgga gctcttcttc ggcatgggcc gcgattgtac gcgggatcgg taagcacggc
     1081 ctaagcgccc gataaggcgc tgcggcgcat cctgccgcga cctgcacgcc cccctttatc
     1141 gaggtgtgcc gtgtccgtcg ttcgcgctgt tcccttcgcc cgacaccggc ttctggccgg
     1201 tctgatctcg atcggcgtgc ttgccattgc gatcgaccat gcggtggcct tcccccttgc
     1261 ccaaggaggg ggcgcgacca cggcgcctct cgtcgaggcg actggccacc tggtcgtgag
     1321 cgaggtgatg acgggtgggg cgagcgcatc cgacgaattc gtcgagctgt acaacccgtc
     1381 gtccacgatg ctgccgctcg agggcctcga actcgtgtac gtcaccgcca gcggggcaac
     1441 cgtcacccgc aaggcttcat gggcggctgg cgccccggga atcccccgcg gtgcccacct
     1501 gctccttgcg aacagtgcag ggatctttgc cggcctggca gacgtggcct atgccaacgg
     1561 gctggcggcg acgggcggta gcgtcgcgct gcgggtgatc ggcgccgcca ccgcgatcga
     1621 cgcggtcggc tggggtagcg cgacgagtac ctggctcgag actcgaccgg cgcccgcgcc
     1681 tgccgccggc agcagcctcg aacggcttcc cggggcctcg ctgggatctg gccaggacac
     1741 gaacgacaac ctgatcgact tcgtcatcca gtcagctcca gatccgcaga acagcggctc
     1801 tccgcccatt ccagttccaa gcgacagtcc cgtgcagacc ggctcaccgg ccccgtcagg
     1861 atcggctgag ccgagtgcca gccaaacgac cgagccatcc gtggagccca gcctcaccgg
     1921 cacgccggcg gcgagcgcga ccgcgactcc gagcgcgacc gtgactccga ggccgacggc
     1981 gacgcctgcg ccgactgcga ccccgacgcc cacgcccagt ccaaccgctg cgccgctctc
     2041 catcggtgac gcgcgctccc aggccgacgg caccacggtg accgtcgagg gcgtgacgct
     2101 gaccgccagc gacttcaccg acgggggtgg ctacctggtc gatgcgaccg gcggcatcgc
     2161 cgtgctcgtc tccgacggca cattcggtcg cggccagcgg ctgcgtgtca ccggcacgat
     2221 cgacgatcgg tatgcgcagc gcaccatccg ggccactgcc gacgcgatca ctaacatggg
     2281 cgttggcagc gaaccgttgg cggcggatac gcccaccggc tcgatcggag agggcactga
     2341 gggccgcctg gtcgaggtca gcggcctgat catcagctcg gtcacgaccc taagcacggg
     2401 cctggcctgg gaccttgatg acggcagcgg cgcaatccgc atcctcgttg gcacggccac
     2461 ggccattgac acctcgacct gggttcgcgg cgtcgggctc aggctcatcg gcgtggtggg
     2521 ccagcgggat tcgagcggga ccggcacatc gggcttccga atccagcctc gcgaaacggc
     2581 tgacctcctc acggtggagc ccatcgccac ccccagtcca acgcccacgc ctcagccgac
     2641 ggcaaccccg acgccgtccg gttcaccgag tccctcagcg acaccgaccc ccggctcccg
     2701 gctcgtttcg atcgccgatg cccgggccgc cacggccggc acgcacctgc gcatccgcgg
     2761 tgtggtgacg gcgccgtccg gcttgatcga caccggcagc gcggtggttc aggatgcgac
     2821 ggcggggatt ctggtccgcc tcggaacgga cgtcggcagc ctgtccctgg ggcagctggt
     2881 tgagctcgat gggacacgag ccaccaagac cggcatgctg agcctgcgcg tcgccacgcc
     2941 gcctgtacag ctcgggacgc aggcagagcc tgagccgatg cgtcgcgcca ccggcgcgct
     3001 tggcgagagc caggaggcgc gcctggtcat cgtgcgcggt tccatcagca cggcgatcag
     3061 caggccgcgc ggcgggaatg tctcattcgc gatcgacgat ggctcagggc cgatccgcgt
     3121 cacgatctcc tcgcgcagcg gcatctccga cgggtcgctg acccgtggcg cctggctcga
     3181 gctgcgcgcc gtgctggctc aggagacgac cggctctgcc ccgtcgagcg gctaccggct
     3241 gtggccacgg gtcgcgagtg acctccgcgt gatcgctgcg ccggtggcgg gatccaccca
     3301 atcggccccg tgctgtgcgt ctgtccagac gaacgactat ccgcttccag atagcgcagt
     3361 cgccgatgcc ctgctgggcc aggctgccgg accgccagga caggaggtga ccccggtgct
     3421 ggcccgacct cagccgacga gctctcctcg accgacctcc tcaaccgggc aagaggccaa
     3481 ggcagcgagc gaccagcgag cgcctgctgg agggctggtc gtcgccggca tgggactcgg
     3541 cacgctggcc gggctgctgg cgtggttcgg gcggcgacgg gcccccgacg acgacgacga
     3601 gccgggcagc atcgacgcgg agggcggtcc acgcttgtcc gtgctgcggg ccgaggggaa
     3661 tgacgctcga aacgagcggg gtatactccc gccgacctga gcgtcccacg ctcgcacgag
     3721 gagctcgccg aggccttgac cgatctctcg caagccgacg cgccgcgcac cggcggtcgc
     3781 cagtaccaca tcgcgctgga gcggggtgag ctggccgagt acatcgtgct ggtgggagac
     3841 cccggacgcg tggccaaagt cgcgtcgctc tgggactcgg tggagctcaa ccggagcaat
     3901 cgagagatca ccaccgcgac cggcacctac cagggcatgc ggatctcggc catgtcgacc
     3961 ggaatgggca cggacaacgt cgagatcgtg ctggccgagg tgatggagat cacggatcag
     4021 cccacgctga tccgcgtcgg ttccagcggc gcactccagc ccgagatcgc gcttggcgac
     4081 ctgatcgtgt cgatcggggc cgtccgcctc gagaacacga ccgacttcta cgtgcacccc
     4141 ggataccctg caattgccca tcgagacgtg gtctgggcac tcgaggcggc ttgcctcgag
     4201 acgggtgctc ggtaccatgt tggaatgaca gcgacggcga gcgggtttta cgctccgcag
     4261 ggaagggcga tgcgcacgct cccggttcgc tatcctgatc tcgcggagga gctgcgacgc
     4321 cagcgcgtcg ccaacctcga aatggagtcg tcggcactct tcgtgttggc aggactcgcg
     4381 gggttgcgcg ccgggacggt gtgtgcggcc tacgcacagc gcgtcgatgg gacgtttctg
     4441 gaaggcgcgg cgaaggaggc cgcagaggca cgctgtatcg atgtcggact ggccggcatc
     4501 cacctgctct gggt
//
LOCUS       DATCQR010000026         1995 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_109859, whole genome shotgun sequence.
ACCESSION   DATCQR010000026 DATCQR010000000
VERSION     DATCQR010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1995
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109859"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(37..1281)
                     /locus_tag="VIN32_00925"
     CDS             complement(37..1281)
                     /locus_tag="VIN32_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006590949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEY8921171.1"
                     /translation="MTVTPSIDGARLLEEQLAQASPDLLRELLTTFINTLMSAEADAV
                     CGAAYGTVSEDRVNRRNGYRSRDFDTRAGTLDLAVPKLRQATYFPEWLLERRKRAERA
                     LTSVVATCYLLGVSTRRMDKLVQSLGITTLSKSQVSEMAKDLDTHVDQFRTRRLEDAG
                     PFTFVAADGLVLKVREGGRVVPVHALVATGVDSDGHRQILGVQVTTSEDGAGWLAFFR
                     DLVARGLSGVKLVTSDAHAGLVAAITATLPGISWQRCRTHYAANLMTITPKSSWPWVK
                     ALLHSIYDQPDAESVCAQFDRVVDALTEKFPAVAEHLEENRADVIAFAKFPKEVWRQI
                     WSNNPNERLNREIRRRTDVVGIFPDRGSIIRLVGAVLAEQHDEWAEGRRYLGLDVLTR
                     SQAVDTNLEEATNDLTMQAITA"
     gene            1462..1686
                     /locus_tag="VIN32_00930"
     CDS             1462..1686
                     /locus_tag="VIN32_00930"
                     /inference="COORDINATES: protein motif:HMM:NF015799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANTAR domain-containing protein"
                     /protein_id="HEY8921172.1"
                     /translation="MAGRWDGLCGLAEQQLRIALQGHIAIEQATGALAQRDTLGIDEA
                     LMRLLARAHATQQTVADVAQAKLNEASPEA"
BASE COUNT          323 a          631 c          700 g          341 t
ORIGIN      
        1 gtggtgtacg aggtctgatc cctcgtggtc gtgcggttag gccgtgatgg cttgcatggt
       61 caggtcgttg gtcgcctcct cgaggttggt gtcgacggcc tgggatctgg tgaggacgtc
      121 gagtccgagg tagcgccgac cttcggccca ctcgtcgtgc tgctcagcca ggacggcgcc
      181 gacgagtcgg atgatcgatc cccggtcggg gaagattccc acaacgtcgg tgcggcgacg
      241 gatctcgcgg ttgagtcgct cgttggggtt gttcgaccag atctggcgcc agacttcttt
      301 ggggaacttc gcgaacgcga tcacgtcggc gcggttctcc tcaaggtgct cggcgacggc
      361 aggaaacttc tcggtcagcg cgtcgacgac gcggtcgaac tgggcgcaga ccgactcggc
      421 gtcgggctgg tcgtagatcg agtgcagcag cgctttgacc cagggccacg agctcttggg
      481 ggtgatggtc atcaagtttg cggcgtagtg ggtgcggcac cgttgccagc tgatgccggg
      541 cagggtcgca gtgatcgcgg cgaccagacc ggcgtgcgca tcgctggtca ccagcttcac
      601 tccggacagg ccgcgggcga ccagatcccg gaagaaggcg agccagccgg cgccatcttc
      661 gctggtggtg acctggaccc cgaggatttg tcggtgtccg tcggagtcga caccggtcgc
      721 gacaagtgcg tgcacgggca cgacgcggcc accttcgcgg accttgagca cgagcccgtc
      781 ggcggccacg aacgtgaacg gaccagcgtc ctcaagccgg cgggtgcgga actgatcgac
      841 gtgggtgtcg aggtccttgg ccatctcgct gacctgactc ttggacaggg tcgtgatccc
      901 gagtgactga accagcttgt ccatccggcg cgtgctgacc ccgagcaggt agcaggtcgc
      961 cacgaccgac gtcagtgctc gctcggcacg cttgcggcgt tccagcagcc attcggggaa
     1021 gtaggtggcc tgccgcagct tgggaacggc caggtccagg gtgccggcgc gggtgtcgaa
     1081 gtcgcgcgat cggtagccgt tgcggcggtt gacccggtcc tcagacaccg tgccgtaggc
     1141 cgcaccgcac acagcgtcgg cctcggccga catcaaggtg ttgatgaagg tggtcagcag
     1201 ctcgcgcagc agatcagggc tggcctgggc cagttgttcc tcgagcaggc gtgcgccatc
     1261 gatacttgga gtgacggtca tcgtcgtgtc cttcttcgag tcggttgtag gagatcactc
     1321 gaaggatcac gcggtggccg tcgcctactt agacgacacg ctcaccgacg aatccgtaca
     1381 ccactctgct ggacgcaact ccggaatctc ggggtctcca gcggggtcaa agcacggtcc
     1441 ccgcggatat catccgttgc catggctggc aggtgggacg ggctatgcgg gctcgccgag
     1501 cagcagctcc ggatcgcact ccagggccat atcgcgatcg aacaggccac aggcgcgctg
     1561 gcccagcgcg acaccctcgg gatcgacgag gccctcatgc ggctcctcgc tcgggcgcac
     1621 gccacacagc agactgtcgc cgacgtggca caggcaaaat tgaacgaagc atccccggag
     1681 gcatgacgat gcccgccgga gtctcgggtc tccagcgggc gagctcgccg acgtggtcgc
     1741 gccccgcacg tctgcgacgg cggggcgctg ggacccggtg agtgtccccc aacactcggg
     1801 ctcttgaccg cggacagtag ttcctggttg ggccggcgtc ccagacctcg atgggccacg
     1861 ggtgacgccc cacgacgcga gacggggggc ccgggccgcg gggcgctgac ctccgggcat
     1921 ggcatggccg gaggtgagcg ccaaccctac gcctgcgacc tcacaggtaa cacctgcgac
     1981 acctcagacg tgagt
//
LOCUS       DATCQR010000027         8732 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_1693, whole genome shotgun sequence.
ACCESSION   DATCQR010000027 DATCQR010000000
VERSION     DATCQR010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 8732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8732
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1693"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="VIN32_00935"
     CDS             complement(<1..239)
                     /locus_tag="VIN32_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012190301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HEY8921173.1"
                     /translation="MFYLQRGQVPHKRHTQFRKPDGSLYAEELFGVEGFSGKASLLYH
                     HTPPTQTNRIEKVRDVSLEPWTDDEAGPHRHHLVNS"
     gene            309..794
                     /locus_tag="VIN32_00940"
     CDS             309..794
                     /locus_tag="VIN32_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007357880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY8921174.1"
                     /translation="MFNVTRLNHAVLWVRDADKAAEFYHRIFGFEELERPQGMRAAFM
                     RSPSGGNHHDLGLFEVGAEAPRPPRGSVGLYHLAWEVGTIEDLATAAKALSEAGALGG
                     ASDHGVSKSLYGRDPDGNEFEIMWQVPREAWGEYADSGVVLPLDLEAELERWGTKGAV
                     Q"
     gene            823..1893
                     /locus_tag="VIN32_00945"
     CDS             823..1893
                     /locus_tag="VIN32_00945"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HEY8921175.1"
                     /translation="MSDLVPVIHSDAHLAHSGLIELASGVEIPCWESPERVLAIEAAL
                     AADGGYAFEQPTEHGRSPILAVHDAKMVEIFEHAWTDALAAGATDGSRAFFPDVFLVA
                     AYGQQMALTGMPAGAHHRLGAYLFDTATPIVGGTWDAALAAVDVALSTAERVVGGAPL
                     AFGLCRPPGHHAARGMLGGYCYFNNAAITAEWLRREGGARRVAILDIDYHHGNGTQQI
                     FWERGDVLYLSLHADPARAYPYFSGYAGERGAGNGARLTRNWPLPAGTAFDAYVVALA
                     EALQMIVAFAPDAPLVISAGFDTFERDPIGDLGLRTPDYREIGHLMAGLGMPMIALLE
                     GGYAVDALGANAVALLSGLRGA"
     gene            1890..3086
                     /locus_tag="VIN32_00950"
     CDS             1890..3086
                     /locus_tag="VIN32_00950"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8921176.1"
                     /translation="MTARFQGRPLWPAYLAMFTFTLGQWASGVAIPLHVQSLGGSLTE
                     AGTIAAIRFGLGAIFSLPFGAVADAWGTRRTVTVAVIGSALVNLVPLLAQTTGTTAPL
                     YAWAVLSGLTSSLFLPAIGAYVAGSAEETRRGSAFGWMTLGTHSGIALGPAVGGLAWQ
                     HLGIPQTYLVATTLALVALAGPILMRGDIRTRVDVRGLPGLVAVLARDRVLVGCWIAA
                     FGIGVPWGAVSGLFPLFGTSIGLAAGSIGLLLALQSITNGLSRVPLGRLIDRRAVPPL
                     AMAGGCVAYAGATSLLGLQSGPLGIAAVFIIGILALAFTLMMIQVVISERAPAELRAT
                     GLGGYSTALSAGLGIGPFVAGAVAQSGGFGIGYASVAAIGAVVAGAAAVLLLGARRVT
                     GARQPR"
     gene            complement(3099..3530)
                     /locus_tag="VIN32_00955"
     CDS             complement(3099..3530)
                     /locus_tag="VIN32_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018189005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY8921177.1"
                     /translation="MITRLNVASIYVLDKEEALDFYVNKLGLEVGNDIRQGDYRWLTV
                     RVPGEGATEVSLEQPGPPLHDEATAAQLRELITKGALGGLVFITDDARGLYETLKARG
                     ITDFTQEPTEHFYGTDMGLRDPFGNPIRILQQGKVAQEATA"
     gene            complement(3625..4026)
                     /locus_tag="VIN32_00960"
     CDS             complement(3625..4026)
                     /locus_tag="VIN32_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020515947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEY8921178.1"
                     /translation="MSQDVEDLNRRLLRARDAMDRSYAEPLDVRAVSAVACLSEAYFI
                     RTFRSVFGETPHRYLQRRRVERSMFLLRETDRSVTDVCLDVGFTSLGTLSRMFREIVG
                     ETPSGYRMGHGPIVAPNCVQLAAMRPLRAAE"
     gene            complement(4036..5475)
                     /locus_tag="VIN32_00965"
     CDS             complement(4036..5475)
                     /locus_tag="VIN32_00965"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HEY8921179.1"
                     /translation="MTTETVATSLTRMNAVERRLTEHAARSLPAGLTDPDPGASERWE
                     AGQVWAHLAEFPGYWLDQIRAILAARAGGAQGPIPFGRMKTDASRTDPIERERRTAPG
                     ELLQRVTAKLGELSAELTALPDDAWSASGLHPTLGEMSLEGIVERFVLAHLEEHADQL
                     DGLELVTVDEIRAAAERIRGVALHTPLLRWDDRTWLKPESLQPVGAFKMRGAYNAVAS
                     LTDAERARGVVTYSSGNHAQAVARAARLMGAHATIVMPEDAPPVKVEGVRRDGAEIIV
                     AGRTGEERHAIALELVERDGRVMIEPYDDRRIIAGQGTCGLEIAEDLPAVTSVLIPVS
                     GGGLSAGIATAIKALAPNARVIGVEPELAADARESLAKGEIVRWDGSLTTRTMADGLR
                     VEHLGWLPFLHLRRFMDEIVTVSEEEMAEAMRQLATRGRLVVEASGAAGMAAHLSGAA
                     PQPEGDDHRVIVISGGNVDPIAFAAILRG"
     gene            complement(5472..6479)
                     /locus_tag="VIN32_00970"
     CDS             complement(5472..6479)
                     /locus_tag="VIN32_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019421081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEY8921180.1"
                     /translation="MKIAHVRETHAPAGTPWRLAAALEPGDSPQRWLDLDPARRDQVA
                     ADQRLAHNSALFRQPLTSLDDLLARGLRVSTLGEIVAGAGADAHTLDAADLAFGPPIL
                     NPPSLRDFYAFEGHVKTMWERRGGTVPEAWYRIPIFYFSNVSEIRGPVEPVWCPAASQ
                     ELDYELEVAALVDTPVSDLSAERAEEAIGGYLVFNDWSARDLQREETTVRLGPAKGKD
                     FASSIGPWLVTPDELADARRGKGYDLAMTATVNGTELSRGSWGDAQFSFGEMIARASA
                     DARLRPGDLLGSGTVGTGCLLEVKDEILKRYLEPGDVVTLTVERLGVLTTPIVERPGD
                     R"
     gene            complement(6502..7635)
                     /gene="hppD"
                     /locus_tag="VIN32_00975"
     CDS             complement(6502..7635)
                     /gene="hppD"
                     /locus_tag="VIN32_00975"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HEY8921181.1"
                     /translation="MTDTLARPAASDAAADFLPLKGIDHVEFWVGNAKQAAAYYRALW
                     GFTPVAYAGLETGVRDRASYVLVQNDIRFVFTAPLTDTGELAEHVRRHGDGVHDIAFA
                     VDDVASAWRETTARGAVSALEPAEVTGEKGVLRRSAIHTYGEVLHSFIDRSDYTGTFA
                     PGYHRIAKPPAAATGQQLLEVDHCVGNVALGDMNKYVDFYRDVLGFSQLIHYDDKVIH
                     TEFSALMSKVMQSGNGRIKFPINEPAVGKRKSQIQEFLDFYLEAGTQHIALRTEDIVG
                     TVRALRSRGVEFLGMPHEYYAALPERVGDVGIPMDLLEELGIEADRDEEGYLLQIFTR
                     PVQDRPTFFFEIIERHGSRGFGVGNFKALFEAIEREQEKRGNL"
     gene            complement(7632..8369)
                     /locus_tag="VIN32_00980"
     CDS             complement(7632..8369)
                     /locus_tag="VIN32_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018110336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEY8921182.1"
                     /translation="MPQIHLRAEPGDYAPLVLLPGDPNRARKIAERFDGGLGKARQVT
                     EHRALYGWTGTYQGHPVSVQTSGMGTPSFAIVAEELLRLGATQLIRVGTCGGIAPGLR
                     TGDLVVALSACPTDGATNTYLHGDRYAPTADFALTHTLVHAAEEAGIPVRVGPVASVD
                     VFYNTDADYFSKWRDRGVLAFEMEASVLFYLAARAGVQAACLLTVSDVLNEEEGTEET
                     YMSLEDLDAAVERMIEVALTAAVQSAP"
     gene            complement(8397..>8732)
                     /locus_tag="VIN32_00985"
     CDS             complement(8397..>8732)
                     /locus_tag="VIN32_00985"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HEY8921183.1"
                     /translation="RSDRQQLDLHLLLQERHRSVLAGEPTAFVEADLDLHAFVAGVAR
                     SPLHAALERMAGVGLRTDMGGRARRLALDEDLDELHRTLVDAIDASDADAARTAARAI
                     AIAEGAAPD"
BASE COUNT         1236 a         2994 c         3049 g         1453 t
ORIGIN      
        1 gagttgacca ggtggtgccg atgcgggccg gcctcgtcat cggtccaggg ctcgagcgag
       61 acatcccgaa ccttctcgat ccggttggtc tgggtcgggg gcgtgtggtg gtagaggagg
      121 ctcgccttgc cggagaagcc ctcgacgccg aacagctctt cggcgtacag gctgccgtcc
      181 ggcttgcgga actgagtgtg gcgcttgtgc ggcacctggc cgcgctggag gtagaacacg
      241 gtgcggccat tctagagtcc acgtgccgat ctcgatccct tcgcgggctg cgctagactc
      301 gacgctccat gttcaacgtg acccgactca accacgccgt cctctgggtc cgcgacgcgg
      361 acaaggcggc agagttctac caccgcatct tcggcttcga agagctggag cggccgcagg
      421 gcatgcgcgc cgcgttcatg cgctcgccga gcggcggcaa tcaccacgac cttggcctgt
      481 tcgaggttgg cgcggaggcc cctcgcccac cgcgtggctc agtggggctc taccacctgg
      541 cctgggaagt cgggacgatc gaggacctcg caaccgccgc caaggcactc tccgaggctg
      601 gagccttggg tggagccagc gaccatggcg tgagcaagtc gctctatggc cgcgatcccg
      661 acggcaacga gttcgagatc atgtggcagg ttcctcgcga ggcgtggggc gagtacgccg
      721 attctggcgt cgtgctgccg ctcgacctcg aggccgagct ggagcggtgg ggcaccaagg
      781 gcgccgtcca gtaggaaggc atcggtacac tcgcaggcgt ccgtgtccga cctcgtcccc
      841 gtcatccatt ccgatgcgca cctggcccac agcgggctga tcgagctcgc cagcggggtc
      901 gagatcccct gctgggagtc gcccgagcgg gtgcttgcga tcgaggcggc gctggccgcc
      961 gacggcggct acgccttcga acagccgacc gagcatgggc gctcgccgat cctggcggtc
     1021 cacgacgcga agatggtcga gatcttcgag catgcctgga ccgatgcgct cgccgccggc
     1081 gccaccgacg gctcgcgggc gtttttcccc gatgtcttcc tggtggcggc ctatggccag
     1141 cagatggcgc tgaccggcat gccggccggg gcgcaccatc ggctgggggc gtacctgttc
     1201 gacaccgcca cgccaatcgt tggcggcacc tgggacgcgg cgctggcggc cgtggacgtg
     1261 gcgctgtcca cggccgaacg ggtggtggga ggcgctcccc tcgcctttgg gctgtgccgg
     1321 ccgccggggc atcacgccgc gcgcggcatg ctgggcggct actgctactt caacaacgcc
     1381 gcgatcacgg ccgagtggct gcgacgcgag gggggcgctc gccgcgtcgc gatccttgac
     1441 atcgactatc accacggcaa cggcacgcag cagatcttct gggagcgggg agatgtgctg
     1501 tacctctccc tgcacgccga cccagcgcgg gcatacccgt acttctccgg gtatgccggc
     1561 gaacgcggtg ccggcaacgg cgcacggttg acgcgcaatt ggccacttcc cgcggggacg
     1621 gcatttgatg cgtacgtcgt ggcgctggct gaggcgctgc agatgatcgt cgccttcgcg
     1681 cccgatgcac cgctcgtcat ctcggcagga ttcgacacct tcgagcgcga cccgatcgga
     1741 gacctggggc tgcgcacccc ggactaccgg gagattggcc atctcatggc tgggctgggc
     1801 atgccgatga tcgccctgct ggagggcggc tatgcggtgg atgcgctggg ggccaacgcc
     1861 gtggcgctgc tctccggcct gcgcggcgcg tgacggcgcg gttccagggg aggccgctct
     1921 ggccggccta cctggccatg ttcaccttca ccctcggcca atgggcgtcg ggggtggcaa
     1981 ttccgctcca cgtccagtcg ctgggaggtt ccctgaccga ggccggcacg atcgccgcca
     2041 tccgctttgg cctcggcgcc atcttcagcc tgccgttcgg cgcggtcgcc gatgcctggg
     2101 ggacccgacg cacggtaacc gttgcggtga tcgggagcgc gctcgtcaac ctggtcccgc
     2161 tcctcgccca gacgaccggg accacggcgc cgctgtatgc ctgggcggtc ctctcgggac
     2221 tcacctcgtc cctcttcctg ccggcgatcg gggcctacgt ggccggaagc gcggaggaga
     2281 cgcggcgcgg atcggccttt ggctggatga cgctcggaac gcatagcgga attgcgctgg
     2341 gtcccgcagt cggcggcctc gcctggcagc accttggcat cccgcagacc tatctcgtag
     2401 ccacgaccct cgcgttggtg gcgctggccg gaccgatcct gatgcgtggc gatatccgga
     2461 ccagggtgga cgtcaggggg ctgcccggcc tggtcgccgt gctggctcgc gaccgggtgc
     2521 tggtcgggtg ctggattgcc gccttcggga tcggcgtgcc gtggggcgca gtcagcggcc
     2581 tcttcccctt gttcgggacc tcgattgggc tggccgccgg ctccatcggc ctgttgctgg
     2641 cgctccagtc catcacgaac ggcctctcgc gggtgccact cggacggctc atcgaccgcc
     2701 gggcggtgcc tcccctggcc atggctggcg ggtgcgtcgc gtatgccggg gcgacctccc
     2761 tgctcggcct gcagagcggt ccgctgggga tcgcggccgt cttcatcatc ggcatcctgg
     2821 cgctcgcctt cacgctgatg atgatccagg tcgtgatcag cgagcgcgcc cccgccgagc
     2881 tgcgggcgac cggcctgggc ggctactcga ccgcactgtc ggcggggctg gggatcggtc
     2941 cgttcgtggc cggcgcggtg gcccagtcgg gcggcttcgg gatcggctat gccagcgtgg
     3001 cggcgatagg cgccgtggtt gccggcgcgg cggcggttct gctgctggga gcgcggcgag
     3061 tcaccggcgc gcgccagccg cgctgagggt ccggaccgtc aggccgttgc ctcctgggcg
     3121 acctttccct gctgcaggat ccggatgggg ttgccgaagg gatcgcgcag gcccatatcg
     3181 gtgccataga agtgctcggt gggctcctgg gtgaagtcgg tgatgccgcg tgccttgaga
     3241 gtttcgtaca ggccccgggc atcgtccgtg atgaagacga gaccacccaa ggcgcccttg
     3301 gtgatcagct cgcggagctg cgcggccgtc gcctcgtcgt gcagtggcgg gcccggctgt
     3361 tcgagggaga cctcggtcgc cccctcgccg gggacgcgga ccgtcagcca gcggtagtca
     3421 ccctgtcgga tgtcgttgcc cacctcgagt ccgagcttgt tgacgtagaa gtcgagcgcc
     3481 tcctccttgt cgaggacata gatcgaagcg acgttcagcc tggtgatcat gagatctcct
     3541 ctcctgtgcg agttcgattg gcgtgacttt gatcctaggg agccgccacg gcctcggctt
     3601 ctccaaaact gctcgattgc atcctcactc cgccgcgcgt agcggtcgca tcgccgccag
     3661 ttggacgcag ttgggcgcga cgatcgggcc atggcccatc cggtagccgg acggtgtctc
     3721 gccgacgatc tcgcgaaaca tccggctgag cgtccccagg ctcgtaaagc cgacgtcgag
     3781 gcagacgtcg gtcacgctcc ggtcggtctc gcgcagcagg aacatggaac gctccacccg
     3841 ccgccgctgc aggtagcggt gaggcgtttc cccgaacacg gagcggaagg tgcggatgaa
     3901 gtaggcctcg gagagacatg ccaccgcgga cacggcgcgg acatcgagcg gctcggcgta
     3961 ggagcggtcc atggcgtcgc gggcgcgcag caggcggcgg ttcagatcct cgacgtcctg
     4021 gctcacgagg gaacgctacc cgcgcaggat cgcggcgaag gcgatggggt cgacgtttcc
     4081 gccgctgatc acgatcacgc ggtggtcgtc cccctccggt tgcggcgccg cacctgacag
     4141 atgcgctgcc atgccggccg cgccggatgc ctcgaccacg aggcggccgc gcgtcgcgag
     4201 ctggcgcatc gcctcggcca tctcctcttc gctgacggtc acgatctcgt ccatgaagcg
     4261 gcgcaggtgg aggaagggga gccagccgag gtgctcgacg cgcagcccgt cggccatggt
     4321 gcgggtcgtc agggagccgt cccagcgcac gatctcgccc ttggccagcg actcgcgcgc
     4381 gtcggccgcg agctcgggct cgaccccgat gacgcgggcg tttggggcca gtgccttgat
     4441 cgcggtggcg atcccggccg agagcccgcc gccactcacc gggatcagga cgctcgtgac
     4501 cgcgggcagg tcctcggcga tctccaggcc acaggtcccc tgccccgcga tgatgcgccg
     4561 gtcgtcgtat ggctcgatca tcacccgacc gtctctctcc accagctcca gcgcgatcgc
     4621 gtggcgctcc tcgcccgtcc gccccgcgac gatgatctcg gcgccgtcgc gacggacccc
     4681 ctcgaccttg accggcgggg cgtcctccgg catgacgatg gtggcgtggg ctcccatcag
     4741 gcgggctgcc cgggcgaccg cctgggcatg gttgccgctt gagtaggtga ccacgccgcg
     4801 cgctcgctca gcgtcggtca gcgaggcgac ggcgttgtac gcgccgcgca tcttgaaggc
     4861 gcctaccggc tgcaggctct ccggcttgag ccatgtgcgg tcgtcccagc gcaggagcgg
     4921 cgtgtggaga gccacccctc ggatccgctc ggcggcggcg cggatctcgt cgacggtcac
     4981 cagctccaga ccgtcgagct gatcggcatg ctcctccagg tgggcgagga cgaatcgctc
     5041 gacgatcccc tccagtgaca tctccccgag ggtcgggtgt agcccgctcg cgctccaggc
     5101 atcgtccggc agggcggtca attcggccga cagctcgccg agcttggcgg tcacgcgctg
     5161 gaggagctcc cccggggccg tccgccgctc ccgctcgatc ggatcggtcc ggctggcatc
     5221 ggtcttcatg cggccgaagg gaatcggccc ctgcgcgccc ccggcccgcg cagcaaggat
     5281 ggcgcggatc tggtccagcc agtagccggg gaactcggcc aggtgcgccc acacctgccc
     5341 cgcctcccag cgctcggagg cgccgggatc gggatcggtc aggccggcag gcagggatcg
     5401 cgcggcatgc tcggtcagcc gtcgctcgac ggcgttcatt cgcgtgagcg aggtggcaac
     5461 ggtctcggtg gtcatcggtc ccctggccgt tcgacgatgg gggtcgtcaa tacgccgagc
     5521 cgctcgaccg tcagcgtcac gacatcgccg ggctcgaggt atcgcttcag gatctcgtcc
     5581 ttgacctcca gcaggcagcc ggtgccgacg gtccccgagc cgaggaggtc gccggggcgc
     5641 agccgcgcat cggcgcttgc tcgggcgatc atctcgccga agctgaactg ggcatcgccc
     5701 caggacccgc gcgacagctc cgtaccgttg accgtggcgg tcatggcaag gtcgtaaccc
     5761 tttccgcgcc gcgcgtctgc aagctcgtcg ggggtgacca gccagggacc gatggaggac
     5821 gcgaagtcct tccccttggc cggtccaagg cgaacggttg tctcctcgcg ctggaggtcg
     5881 cgtgcgctcc agtcgttgaa gacgaggtag ccaccgatcg cctcctccgc gcgctccgcg
     5941 gagaggtcgc tcaccggcgt gtcgaccagg gccgccacct cgagctcgta gtccagctct
     6001 tgcgacgccg ccggacacca caccggttca accgggccgc ggatctccga aacgttggag
     6061 aaatagaaga tcgggatcct gtaccacgcc tcgggaacgg tgccgccgcg gcgctcccac
     6121 attgtcttga cgtgcccctc gaacgcgtag aagtcgcgca gcgatggcgg attcaggatc
     6181 ggtggcccga atgccaggtc cgctgcgtcc agcgtgtgag cgtccgcgcc ggctcccgcc
     6241 acgatctcgc ccaatgtcga cacgcgcagt ccgcgcgcca gcaggtcgtc caggctggtc
     6301 aacggctggc gaaacagcgc cgagttgtgc gccagccgct ggtcggcggc aacctggtct
     6361 cgccgagcgg ggtccaggtc gagccaccgc tgcggcgagt ccccgggctc gagtgcagca
     6421 gcgagccgcc acggcgtccc cgccggagcg tgcgtctcac gcacgtgggc gatcttcatg
     6481 gtcgatggag tctacgaggg ctcagaggtt gccgcgtttc tcctgctcgc gctcgatcgc
     6541 ctcgaagagg gccttgaagt tgccgacccc aaagccgcgc gacccgtgcc gctcgatgat
     6601 ctcgaagaag aaggtcgggc gatcctgcac cggcctggtg aagatctgga gcaggtagcc
     6661 ttcctcgtcg cgatcggcct cgattccgag ctcctcgagg aggtccatgg ggatgccgac
     6721 gtcgccgacg cgctccggca gcgccgcgta gtactcgtgc ggcatgccga ggaactccac
     6781 tccccgtgag cgaagggcac gcaccgtgcc gacgatgtcc tcggttcgga gagcgatgtg
     6841 ctgggtcccc gcctcgaggt agaagtccag gaactcctgg atctggctct tgcgcttgcc
     6901 aacggcgggc tcgttgatcg ggaacttgat tcgcccgttg cccgactgca tcaccttgct
     6961 catcagggcg gagaactcgg tgtggatcac cttgtcgtcg tagtggatca gctgcgagaa
     7021 gccgagcacg tcgcggtaga agtcgacgta cttgttcatg tcgcccagcg ccacgttgcc
     7081 gacgcagtgg tccacctcga gcagctgctg tccggtggcc gccgccggcg gcttggcgat
     7141 acggtgatag ccgggggcga aggtgccggt gtagtcgctg cggtcgatga acgagtggag
     7201 cacttcgcca tacgtatgga tcgccgagcg gcgcagcacg cccttctcgc cggtgacctc
     7261 cgccggctcc aacgcgctca ccgctccgcg cgcggtggtc tcgcgccagg ccgatgccac
     7321 gtcgtcgacg gcaaaggcga tgtcgtggac tccgtcgccg tgacggcgga catgctcggc
     7381 cagctccccg gtgtcggtca acggggcggt gaagacgaaa cggatgtcgt tctgcaccag
     7441 gacgtagctg gcgcggtcac ggacccccgt ctcgaggccg gcataggcga ccggcgtaaa
     7501 tccccacagg gcccgatagt aggccgccgc ctgcttcgcg ttcccgaccc agaactcgac
     7561 gtggtcgatc cccttcagag ggagaaagtc ggccgccgcg tccgaggcgg cagggcgggc
     7621 aagggtatcg gtcatggcgc cgattgtacg gcggctgtca gcgccacctc gatcatgcgt
     7681 tcgaccgcgg cgtccaggtc ctcgagcgac atgtaggtct cttccgtgcc ctcttcctcg
     7741 ttcagcacgt cgctgacggt gagcaggcag gccgcctgga ccccggcgcg cgccgccagg
     7801 tagaagagga ccgatgcctc catctcgaag gcgagcaccc ctcggtcgcg ccacttgctg
     7861 aagtagtcgg catcggtgtt gtagaagacg tcgaccgatg ccaccgggcc gacgcgcacc
     7921 gggatccccg cctcttccgc cgcgtggacc agggtgtgcg tcagggcgaa gtcggcggtc
     7981 ggggcgtatc ggtccccatg gaggtaggtg ttggtggcgc catcggtcgg gcaggcagag
     8041 agcgcgacca ccaggtcacc ggtgcgcagg cccggcgcga tcccgccaca ggtcccgacg
     8101 cgaatcagct gcgtcgcgcc gagccgcagc agctcctcgg ccacgatcgc aaagctcggc
     8161 gtccccatgc ccgatgtctg cacgctcacc gggtgtccct ggtacgtgcc cgtccagccg
     8221 tacagggccc gatgctcggt cacctggcgt gcctttccca gccctccgtc gaaacgctcc
     8281 gcgatcttgc gagcccggtt ggggtcgccc ggcagcagca ccagcggcgc gtagtctccc
     8341 ggctccgcac ggagatggat ctgtggcacg cccgataggg tagcgcagca agatccctag
     8401 tcgggtgctg cgccctcggc gatggcgatc gctcgtgcgg ccgtgcgggc ggcatcggca
     8461 tccgatgcat cgatggcatc gacaagcgtg cgatgcagtt catcgaggtc ttcgtcgagc
     8521 gccaagcgtc tcgcccgccc tcccatatcc gtcctgaggc ccactccagc catgcgctcc
     8581 agcgcggcat gcagcggact gcgagcgact ccggccacga acgcgtgcag gtccaggtcg
     8641 gcctcgacga aggctgtcgg ctcacccgcc aggacggacc gatggcgctc ctggagcaac
     8701 aggtggaggt caagctgctg tcggtccgag cg
//
LOCUS       DATCQR010000028         5643 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_140894, whole genome shotgun sequence.
ACCESSION   DATCQR010000028 DATCQR010000000
VERSION     DATCQR010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5643
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140894"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..534)
                     /locus_tag="VIN32_00990"
     CDS             complement(<1..534)
                     /locus_tag="VIN32_00990"
                     /inference="COORDINATES: protein motif:HMM:NF014003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HEY8921184.1"
                     /translation="MTDRPFISFMTDFGVGSSAPAVCRGVMLDIAPDARLVDVTHAIR
                     HFALRDGAFLLARSVPYFPIGVHVAVVDPGVGTARRPIALQAARGDYLVGPDNGLLVP
                     AAMALGGIAGARALENRALWLPSVSHTFHGRDIFSPVAAHLATGTPFESFGPELEPAE
                     VTCLEMPIATPRDGGLDT"
     gene            complement(564..2084)
                     /locus_tag="VIN32_00995"
     CDS             complement(564..2084)
                     /locus_tag="VIN32_00995"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY8921185.1"
                     /translation="MSISIQFLGAVGTVTGSQFLVTAGERQILVDCGMFQGAPEEVSR
                     NRMAFAYDPKTLDALLLTHAHFDHCGRIPALVKAGFRGPIFATAGTADLTEIVLRDAA
                     KLQEEAENRWRKKHPDDAATRDAELASEEAAVENDAQMPAKLRAGPAEGTTMTRTALY
                     SMADVEQTVTQFKPVDYDTEVNLLDGITATFRDAGHILGSAIVELKVIEGAASTTIVF
                     SGDLGRNDTPILRDPTPISHADFVLVESTYGNREHAPHEQAIEELASAINEVATDRGV
                     LLVPSFAIGRTQELVWVLDELYRDKRIPRLPLYLDSPMASRASDVYLRHPEDFDEETA
                     ALLRSGDSPLVYPGETYTNEVDQSKGIRTQPRPLMVVASSGMLTGGRIMHHLKDFLPD
                     PACTLLFIGYQGEGTLGRHIQSGAKVAKIDGVAYDIKCRVRVISGFSAHADEHELDDW
                     LGNFAKAGGTNGADGKPKRVFVTHGDPDAAEAFATRIQAMGLDAHLPTYLETVQLR"
     gene            2131..3216
                     /locus_tag="VIN32_01000"
     CDS             2131..3216
                     /locus_tag="VIN32_01000"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012098854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HEY8921186.1"
                     /translation="MSFPIEPPVEPMLAKPTPELPVGEGWRYEPKWDGFRALVFRDGP
                     DLYLQSRDLKPLDRYFPELLEPLRAVGGPKARFVLDGEIVIARDGGLEFESLLLRIHP
                     AASRVAMLAAETPASFVAFDCLAEGADDLRDAPLDDRRAQLEALLADPPASVLLTPAT
                     SDPAVARHWFDVFEGAGLDGVIAKRAEGRYLPGKREMAKIKHLRTADCVVAGFRWHKN
                     GPGTMVGSLLLGLWNDAGQLQHAGVSSSFTEERRRELVTFLEPYRVDALDGHPWKEWN
                     EWQAAAEGKRMPGATSRWNRGKDLSWEPLRPELVCEVAFDHLQGDRFRHGTTFKRWRP
                     DKSPAECRYDQLEETPPALLADLFGGR"
     gene            3269..4975
                     /locus_tag="VIN32_01005"
     CDS             3269..4975
                     /locus_tag="VIN32_01005"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEY8921187.1"
                     /translation="MSHADPNAAVNELADRFWEGALERDPVYATILGDDRWDDRLPDL
                     GEAGRAADEAAFRETLADAEAIDPTGLDPEQVITRDMLILVASTHLEALEKKQYQLAI
                     NHIFGTQTMPVRIAQYQIADTPDGLERLLARFVAYPAAIEQEIQTLREGMADGRTGAA
                     IPVRKEIEQIERMLALPVDTFPAVAMAHVADDAARERVRVSVQADLYPALQALRDFLA
                     DEYEAQARLEPGISSTPGGEEAYRLGIRMHTTIDTTPDEVHRFGLEDLAHIEAEKDQI
                     AKRAGYRDRVDYARALREDPANHTSSREQIVELAAEQTARAFATAPRYFGRLPRADCE
                     VKAVEEYREREVPPAFYMPPSIDGSRAGQYYLNTYQPEARDLHKIAAITFHEATPGHH
                     FQLAIEMELTGLPAFRTLGSRMAGAAYSEGWGLYCERLADEMGLYQSEAERLGMLDAQ
                     SFRAARLVVDSGLHAFGWSRERAIELMHERGSLPLVDAEIEVDRYTIWPGQALAYKIG
                     QREIERARREVSEFMGDRFDLRAFHDEVLAHGSVPLATLRREIPGWVEAAVTAREGAP
                     AV"
     gene            5200..>5643
                     /locus_tag="VIN32_01010"
     CDS             5200..>5643
                     /locus_tag="VIN32_01010"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8921188.1"
                     /translation="MKRPERIALDGRFVRLEPLAETHRDDLLEAAAQDPVTFRYMGTD
                     LSVGSSAWDAYLADALRPEYVAWATVDVATGRAVGSTRFGDIAPEHGHVEIGWTWIAP
                     SHQRSATNTEAKLLQLTYAFDEVGATRVALKTDGRNLRSQAAIERL"
BASE COUNT          893 a         1937 c         1929 g          884 t
ORIGIN      
        1 tgtgtcgagc ccgccgtcgc gcggcgtggc gatcggcatc tccaggcagg tcacctcagc
       61 gggctcgagc tccgggccga agctctcgaa gggtgtgccg gtggcaaggt gagctgcgac
      121 cggcgagaag atgtcccgcc cgtggaaggt gtgcgacacg cttggcagcc agagggcgcg
      181 gttttcgagg gcgcgggctc cggcgatccc acccagcgcc atcgccgccg ggaccagcag
      241 cccgttgtcg ggtccgacga ggtagtcacc gcgagccgcc tgcagggcga tcggccggcg
      301 ggccgtgccg acccccgggt cgaccacagc cacgtgcacg ccgatcggga agtacggaac
      361 gcttcgtgcc agcaggaacg cgccatcccg cagggcgaaa tggcggatcg catgcgtcac
      421 gtcgaccagt cgcgcgtcgg gggcgatgtc cagcatcacg ccacggcaca ccgcaggggc
      481 gctggagccg acaccgaagt cggtcatgaa cgagatgaag ggtcggtcgg tcatgcgcgg
      541 catgctagcc acccccgggg cactcagcgc agctggaccg tctccaggta ggtcgggagg
      601 tgggcgtcca ggcccatcgc ttggatccgc gttgcaaatg cttcggccgc gtcgggatcg
      661 ccatgcgtga cgaagacccg cttcggcttg ccatcggctc cgttcgtgcc gcctgccttc
      721 gcgaagttgc cgagccagtc gtcgagctcg tgctcgtcgg catgcgcgga gaagccggag
      781 atgacgcgga ctcggcactt gatgtcgtac gcgacaccgt cgatcttggc caccttggcg
      841 cccgactgga tgtgccgccc gagcgtcccc tcaccctggt agccgatgaa cagcagggtg
      901 caggcgggat ccgggaggaa gtccttgagg tggtgcatga tccgcccgcc ggtcagcatc
      961 cccgagctgg cgacgaccat cagtggccgc ggctgcgtcc tgatcccctt cgactggtcg
     1021 acctcgttgg tgtacgtctc gccgggatag acgagcggcg aatcgccgga ccgcagcagc
     1081 gcggcggtct cctcgtcgaa gtcctccggg tgacggagat agacgtcgct ggcgcgactc
     1141 gccatcgggg agtcaaggta gagcggcagc cgcggaattc gcttgtcgcg atacagctcg
     1201 tccaggaccc acaccagctc ctgggtacgc ccgatcgcga agctgggcac cagcaggacg
     1261 ccgcgatcgg tggccacctc gttgatcgcg ctcgccagct cctcgattgc ctgctcgtgc
     1321 ggcgcgtgct cccggttgcc gtaggtcgac tcgaccagga cgaagtcggc gtggctgatc
     1381 ggcgtcgggt cgcgcaggat cggcgtgtcg ttgcggccga gatcgcctga gaagacgatg
     1441 gtggtcgagg ccgcgccttc gatgaccttc agctcgacga tcgctgagcc caggatgtgc
     1501 ccggcgtcgc ggaaggtggc cgtgatgccg tcaagcaggt tcacctccgt gtcgtagtcg
     1561 accggcttga actgggtcac ggtctgctcg acatcggcca tcgagtagag cgcggtgcga
     1621 gtcatggtcg tcccctcggc aggccccgcc cgcagcttgg ccggcatctg cgcgtcgttc
     1681 tcgactgccg cctcctcgct cgccagctcc gcgtcgcgcg tggccgcgtc gtccgggtgc
     1741 ttcttacgcc atcggttttc cgcctcttcc tgcagcttcg cggcgtcacg caagacgatc
     1801 tcggtcaggt cggccgtgcc cgcggttgcg aagatcggtc cacggaagcc ggccttgacc
     1861 agggcgggga tgcgcccgca gtggtcgaaa tgggcgtggg tcaacagcag cgcgtcgagg
     1921 gtcttcgggt cgtaggcaaa cgccatccgg ttgcggctca cctcttccgg cgcgccctgg
     1981 aacatgccgc agtcgacgag gatctggcgt tcaccggcgg tgaccaggaa ctgcgagccg
     2041 gtgaccgtcc ccaccgcccc caggaactgg atcgagatgc tcatcacggc gagtatggcc
     2101 gaaacatcgg cgcgggtatc atcggtgccc atgtcgttcc cgatcgagcc ccccgtcgag
     2161 ccgatgctgg ccaagccgac gccggagctg ccggtcgggg aaggctggcg ctacgagccg
     2221 aagtgggacg gcttccgggc cctcgtcttt cgcgacgggc cggatctcta cctccagtcg
     2281 cgggacctga agccactgga ccgatacttc ccggagctcc tcgagcccct gcgcgcggtt
     2341 ggtggtccga aggcgcgctt cgtgctcgac ggagagatcg tcatcgcgcg cgacggcggg
     2401 ctggagttcg agtcgctgct gctccgaatc cacccggcgg cgtcccgcgt cgcgatgctg
     2461 gcagccgaga cgcccgcctc gtttgtcgcc ttcgactgcc tcgccgaagg ggccgatgac
     2521 cttcgggacg cgccgctcga cgatcggcgc gcgcagttgg aggcgctcct cgccgacccg
     2581 ccggcatcgg tcctgctcac tccggcgacc agcgaccctg cggtggcgcg tcactggttc
     2641 gacgttttcg agggggccgg ccttgacggc gtgatcgcca agcgggctga gggccgctat
     2701 ctgcccggaa agcgggagat ggccaagatc aagcacctgc gtaccgcgga ctgcgtcgtg
     2761 gccggcttcc gctggcacaa gaacgggccc ggcacgatgg tcggctccct gctcctgggc
     2821 ctgtggaacg acgccggcca gctgcagcat gccggtgtca gctcgtcgtt caccgaggag
     2881 cgacgtcgtg agctcgtcac cttcctcgag ccgtaccgcg tcgacgcact tgatgggcac
     2941 ccctggaagg agtggaacga atggcaggcc gcagccgagg ggaagcgcat gccgggcgca
     3001 acctcgcgct ggaatcgcgg caaggacctg tcctgggagc cgctgcggcc cgagctcgtg
     3061 tgcgaggtcg ccttcgacca cctccagggc gaccgcttcc gccatggcac gaccttcaag
     3121 cgctggcgtc cggacaagtc cccggccgag tgccgatacg accagctcga ggagacgccg
     3181 ccggccctgc tcgccgacct gttcggtggt cgatgaccgg gtaaactcct cgccgccgca
     3241 atcgtccaga caaggagacc ctgcgcccgt gagccacgcc gacccgaacg ccgccgtcaa
     3301 cgagctcgca gatcgcttct gggagggagc cctcgagcga gacccggtct acgccaccat
     3361 cctgggcgac gaccgctggg acgaccgcct tcccgacctt ggtgaggcag gcagggccgc
     3421 cgacgaggcc gccttccgcg agaccctcgc cgacgcggag gcgatcgacc ccacagggct
     3481 cgatcccgag caggtcatca cgcgcgacat gctgatcctg gtcgcgagca cccacctcga
     3541 ggcgctggag aagaagcagt accagttggc gatcaaccac atcttcggca cccagaccat
     3601 gccggtccgg atcgcgcagt accagatcgc tgacacgccc gacgggctgg agcggctgct
     3661 ggcccgcttc gtcgcctatc ccgcggcaat cgagcaggag atccagaccc tccgcgaagg
     3721 gatggccgat ggtcgcaccg gagcggcgat tccggtgcgg aaggagatcg agcagatcga
     3781 gcgcatgctg gcgcttcccg ttgacacttt cccggcggtg gcgatggccc acgtcgccga
     3841 cgacgcagca cgtgaacggg ttcgcgtctc cgtgcaggcg gatctctatc ccgcgctcca
     3901 ggcgctgcgt gacttcctgg ccgatgaata cgaggcgcag gcgcgtcttg agcccggcat
     3961 ctccagcacg ccgggtggcg aggaggccta tcggctcggg atccgcatgc acacgacgat
     4021 cgataccacc cccgacgagg tccatcggtt cgggctcgag gacctggcgc acatcgaggc
     4081 cgagaaggac cagatcgcga agcgggccgg gtaccgcgac cgcgtcgact acgcgagggc
     4141 gctccgcgag gatccggcca accacacctc ctcgcgagag cagatcgtgg agctggcggc
     4201 cgagcagacg gcgcgcgcct tcgcgacggc tccgcgctat ttcggccgcc tgccgagggc
     4261 cgattgcgag gtcaaggcgg tcgaggagta ccgcgagcgc gaggtgccac cggccttcta
     4321 catgccaccc tccatcgatg ggtcgcgcgc cggccagtac tacctgaaca cctaccagcc
     4381 ggaggcgcgc gacctgcaca agatcgccgc catcaccttc cacgaggcga cgccgggaca
     4441 ccatttccag ctcgccattg agatggagct gaccggcctg ccagccttcc ggaccctcgg
     4501 ctcgcgaatg gccggcgcgg cctactcgga aggttggggc ctctactgcg agcggctcgc
     4561 cgacgagatg ggcctttacc agtccgaggc cgagcggctc gggatgctcg atgcgcagtc
     4621 attccgggcg gcccgcctgg tcgtcgactc tgggctccac gcattcggct ggagcaggga
     4681 gcgcgccata gagctgatgc acgagcgcgg ctcgctgccg ctggtcgacg cggagatcga
     4741 ggtcgaccgc tacaccatct ggccgggcca ggcgctcgcg tacaagatcg ggcagcgtga
     4801 gatcgagcgc gcgcggcgcg aggtcagcga attcatggga gaccgattcg acctgcgtgc
     4861 cttccacgac gaggtgctcg cccacggctc agtgccgctg gccacgctcc gccgcgagat
     4921 acccggctgg gtcgaagcgg ccgtgacggc gcgcgagggc gcccccgcag tctagggcac
     4981 tagatggtcg ccgccggttc tgccagattc acatgaggtg ggcacaacgc gctcaaccgc
     5041 tccctccgcg cgccaaattc acgcgaggtg tgcatctggc ccgaacttgg acccaaaagg
     5101 cgccaaattc atgcgacgtg acgcgtctgg caattccgtg ggccgcaaca tgctcccggc
     5161 ggtcgcttgg tgtgtatctg acaaattcat tggctgccca tgaagcgccc tgagcgaatc
     5221 gccctggacg ggcggttcgt gcgcctcgag ccgctcgccg agacgcatcg cgacgatctc
     5281 ctggaggcgg cggcgcagga ccccgttacc ttccgctaca tgggcactga cctctcggtt
     5341 ggctccagcg cctgggatgc ctacctggcc gatgccctcc ggccggaata cgtcgcctgg
     5401 gcaacggtcg atgtcgccac cggccgagcg gtggggtcca ctcgcttcgg cgacatcgcg
     5461 ccagagcacg ggcacgttga gatcgggtgg acctggatcg cgccgtcgca ccagcgcagc
     5521 gcgaccaata ccgaggccaa gctgctccag ctcacctacg cgttcgacga ggtgggcgcg
     5581 actcgtgtgg cgctcaagac cgatggacgc aacctccgtt cccaggcggc gatcgagcgg
     5641 ctc
//
LOCUS       DATCQR010000029         5559 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_16876, whole genome shotgun sequence.
ACCESSION   DATCQR010000029 DATCQR010000000
VERSION     DATCQR010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5559
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16876"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            69..722
                     /locus_tag="VIN32_01015"
     CDS             69..722
                     /locus_tag="VIN32_01015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921189.1"
                     /translation="MREPEIWPSMSKGPVVPPELGPTTEQPTVGRPLAELIERADRLT
                     ADEIAALDRSFRNTPAIGLAWTWAYGGEVMWANCYDYEGNWDQLDALKANLDDARAAV
                     WSAAVAAKVPGSDDPGRIELERRWTSIRVNPTLARWSEAADEVLAAPGPTWGAALAVE
                     AAILAFAGGHLDPDRALIRPWEEVIERTIFGVPEKRWWPWTWPPELTSSTGNRDTPS"
     gene            781..868
                     /locus_tag="VIN32_01020"
     tRNA            781..868
                     /locus_tag="VIN32_01020"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:815..817,aa:Ser,seq:tga)
     gene            884..1711
                     /locus_tag="VIN32_01025"
     CDS             884..1711
                     /locus_tag="VIN32_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018724989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY8921190.1"
                     /translation="MDDEALRDNRRLWDRWTEINAGSEFYDVASFRDGRRPIRLADYE
                     REEIGSVEGKTLLHLQCHFGLDTLSWARLGARVTGADFSEAGIAAARELAVEVDLPAT
                     FLVANLYDLPTVLDGEFDIVYTSRGVLGWLPDIEGWARVAAHFVKPGGFFYVTEIHPV
                     AQVFEGEGVEPGELRLAYPYWSHRESLRFEVKGSYADPTASTEGLVEHGWDHSMGEIV
                     TALIDAGLRIDFLHEFDFVEWPVGFTVEGEDGRWRLPPTTKGQLPLFFSLKATKPIA"
     gene            complement(1719..2303)
                     /locus_tag="VIN32_01030"
     CDS             complement(1719..2303)
                     /locus_tag="VIN32_01030"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009766223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEY8921191.1"
                     /translation="MFYTAGWVHVICGCMFCGKTDEMLRLLRRFSIAGRTVVLVKPRL
                     DTRTEDGTVISRSGAHHTAIAVDDSRQIEELVGDADIVAIEEGQFFDERLPDVVGRLA
                     DAGKQVLVTGLDQDFRGIPFGPMPRLMALADQVTKLTAICVVCGEPATRTQRLIDGQP
                     APADSPLIVIGGLGDEKYEARCRLHHEVPPAESG"
     gene            complement(2310..2516)
                     /locus_tag="VIN32_01035"
     CDS             complement(2310..2516)
                     /locus_tag="VIN32_01035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921192.1"
                     /translation="MQRLPVIGQSFAHSAVETVQRAAFEASGPSITIERWERTSHDPP
                     LEAMRSALATELGLPEQGIAVVGD"
     gene            2570..2661
                     /locus_tag="VIN32_01040"
     tRNA            2570..2661
                     /locus_tag="VIN32_01040"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2604..2606,aa:Ser,seq:gct)
     gene            2676..2751
                     /locus_tag="VIN32_01045"
     tRNA            2676..2751
                     /locus_tag="VIN32_01045"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2709..2711,aa:Arg,seq:gcg)
     gene            complement(2772..>5559)
                     /locus_tag="VIN32_01050"
     CDS             complement(2772..>5559)
                     /locus_tag="VIN32_01050"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY8921193.1"
                     /translation="GVNTGEVVAGDPVAGEALVIGDAVNVAARLEQAANPGEVIVGAG
                     TYRLVRDAIEAEPIPALELKGKAEPVEAYRLLGLKEQQPIRRHDTPLVGRQSELRILG
                     EAFERAKREEACHLFTLLGTAGVGKSRLIHEFLQQVAGGARVLRARCLPYGEGITFWP
                     IVELVQAAAGINPADGAETAREKLTTLLEGADERDAIVERIAAAIGLSESAIPSEEIF
                     WGVRKLLETLARPQPLIVVIDDLHWAEPTMLDLVEHIADWSHEAPILLLAVARPEMLD
                     VRPQWGGGKMNATTILLEPLTADDSAELVGNLINDETLARAVQGRIGETAEGNPLFVE
                     ELVAMLVDEGVLRRVNGSWQSDADLSQISVPPTVSALVAARLDRLEPSERDLIGRASV
                     VGKVFQRSAVTELSPPERREELGSRLMTLVRKELVRPDRSGTTGDEAFRFRHILVRDA
                     AYGSLPKEQRADLHARFAAWLEGVAGERQLEYEEVIAYHLEQAHRYRAELGLTDELTR
                     SLAASAASHLRSAGLRALARNDPPATTNLLSRAVALLEDPRQRGELLMHLAAAAVDEG
                     DFPRATAMFDEVKAAARRAQDDLLEMRAELEWMGQARQLIDPSMDQSQMLSLASRVEG
                     LAVERGERSGRIAAEFARADAYLMECRWMDELAALERARELLDPDEDPRHWLRCNVMI
                     CNSLRWGPVPAAEAITRIEAITWVGDTGKVGRAVFTAALRAMLGQFDHARNEARASRE
                     YLEERGMRMRVAGSAMQRSAVEELAGDLGAADLALGEGIDILKSMGEKGVLSTLAGMR
                     AAVLYRLGRSKDAEASVLLAQETGSPQDIATQVGWRNVAAQLAADEGRLADAERVIGE
                     AVELIEPTDFLELRGGAFEALAHVEGRAGRPDAWKAALDRAIAEHERKGNLVAASRLR
                     EQLVAGAPDPVASA"
BASE COUNT          863 a         1902 c         1854 g          940 t
ORIGIN      
        1 gtttggccca ctcccggcgg aggcactggc gggaccgccg cggttgggag tccggaggca
       61 atctcgaaat gcgtgagccc gaaatctggc cctccatgag caaggggccg gtggtgccgc
      121 ctgagttggg tccgaccaca gagcagccga cagtgggtcg gccgcttgcc gagttgatcg
      181 agcgcgcgga tcggctgacg gcggatgaga tcgccgccct cgaccgctcc tttcgcaaca
      241 cgcccgcgat cgggctggct tggacgtggg cgtatggcgg cgaggtgatg tgggcgaact
      301 gctacgacta cgaaggcaac tgggaccagt tagacgcgtt gaaggccaac ctggacgacg
      361 cccgcgccgc tgtctggtcc gccgcagttg cggccaaggt tcccggctcg gatgatcccg
      421 gacggataga actagagcgg cgatggacga gcatacgagt caacccgacc ctggcgcgct
      481 ggtcggaggc cgcggacgag gtcctggccg ctcccggtcc gacgtggggc gccgcgctgg
      541 ccgtggaggc agccatcctg gcctttgccg gcgggcacct cgatcctgac cgtgccttga
      601 tccggccctg ggaagaggtg atcgagagga ccatcttcgg ggtaccggag aagcggtggt
      661 ggccttggac atggcctcca gaactcacgt ccagcacggg gaaccgtgac acgccttcat
      721 gatcgaggct ggtgagggct ttcagctctg gcgtaaggtc ccacgctaga atcacggtcc
      781 ggaggggtgg cagagtggac gaatgcggtg gtcttgaaaa ccgctgaggt gcaagcctcc
      841 gtgggttcga atcccacccc ctccgccact acactccgcg ggcatggacg acgaagccct
      901 tcgtgataac cgccgcctct gggaccgctg gaccgagatc aacgcgggtt ctgagttcta
      961 cgacgtcgcc tcattccgcg atggacgccg cccgatccgc ctggccgact acgagcgcga
     1021 ggagatcggg tccgtagagg gcaagaccct gctgcacctg cagtgtcatt tcgggctcga
     1081 cacgctctcg tgggctcgac ttggcgctcg cgtaactggg gctgacttca gcgaggctgg
     1141 gatcgccgct gcccgggagc tcgctgtcga ggttgacctt ccggcgacct tcctggtcgc
     1201 gaacctctac gacctgccaa ccgtgctcga cggggagttc gatatcgtct acacgtctcg
     1261 cggcgtcctc ggctggctgc cggatatcga gggctgggca agagtggcgg cgcattttgt
     1321 gaagcccggc gggttcttct acgtcaccga gatccatccg gtcgcacagg tcttcgaggg
     1381 cgagggcgtg gagcctggcg agctgcgctt ggcgtatccc tactggtccc accgggaatc
     1441 actcaggttc gaggtgaagg gctcgtacgc ggaccccacg gcctcaaccg agggcctcgt
     1501 cgagcatggc tgggaccact ccatgggcga gatcgtcacg gccctgatcg acgccggtct
     1561 gcgcatcgac ttcctgcacg agttcgactt cgtcgaatgg ccggtggggt tcacggtcga
     1621 gggcgaagac ggccggtggc gtctgccacc caccacgaag ggacagctgc ccctcttctt
     1681 ctcgctcaag gcgaccaagc cgatcgcgtg agctgcgcct agccggattc agccggcggc
     1741 acctcgtggt ggagccggca tcgggcctcg tacttctcgt ccccgagccc cccgatcacg
     1801 atcagcggtg agtcagccgg ggccggttgc ccatcgatca gccgctgggt tcgggtggcc
     1861 ggctccccgc agacgacgca gatggccgtc agcttggtga cctggtcggc cagcgccatc
     1921 aggcgcggca tcggtccgaa cgggatcccg cggaagtcct ggtccaggcc ggtcaccagt
     1981 acctgctttc ccgcatcggc caggcggcca accacatctg gcaggcgctc gtcgaagaac
     2041 tgtccctcct cgatggccac gatatcggcg tcaccaacca gttcctcgat ctgccgcgag
     2101 tcatcgacgg cgatcgccgt gtggtgcgcc ccggagcgcg agatgacggt cccatcctcg
     2161 gtacgggtgt ccagccgcgg cttgacgagc accaccgtgc ggcccgcgat cgagaagcgg
     2221 cgcaggaggc gcagcatctc gtcggtcttg ccgcagaaca tgcagccgca gatcacgtgc
     2281 acccagccgg cggtatagaa cattggctct tagtcgccga ccacggcgat gccctgttcg
     2341 ggcaacccga gctccgtcgc gagcgcgctg cgcatcgcct caagcggggg atcatggctg
     2401 gtgcgttccc agcgctcgat cgtgatcgac gggccgctcg cctcgaaggc ggcccgctgc
     2461 accgtctcga cggcggaatg ggcgaatgac tggccgatga cggggaggcg ctgcacgggt
     2521 ggatctgcct ggcggaaagg gccttgatgc tatcatcggc gccgctcgcg gagaggtcgc
     2581 atagtggcct agtgcggcgg tttgctaaac cgttgaaggg ggtttcctct tccgagggtt
     2641 cgaatccctc cctctccgcc atcgccgtcg cgcatgtgcc cgtagctcag tggatagagc
     2701 gtcaggttgc ggacctgaag gtcggaggtt cgagtcctct cgggcacgcc actcgccagc
     2761 ggcaagctcg ttcaggcgga agcgaccggg tctggggcgc cggcgaccag ctgctcgcga
     2821 aggcgcgatg cggctaccag gttgcccttt cgctcgtgct ccgcgatcgc acgatcgagc
     2881 gctgccttcc aggcgtccgg tcggcccgcc cggccctcga cgtgcgccag ggcctcgaag
     2941 gcccctccgc gcagctccag gaagtccgtg ggttcaatga gctccacggc ctcgccgatc
     3001 actcgctcgg cgtccgccag gcgaccttca tccgcagcaa gctgggccgc cacatttcgc
     3061 cacccgacct gggttgcgat gtcctgcggc gaaccggtct cctgagccag cagcaccgac
     3121 gcctccgcgt ccttgctgcg gccgaggcgg tagaggaccg cggcgcgcat gcccgccagg
     3181 gtcgagagga ctcccttttc gcccatggac ttgaggatgt cgatgccttc gcccagagcg
     3241 agatcggccg ccccgaggtc accggccagc tcttccacgg cactccgctg catggcgctg
     3301 cccgcgacac gcatccgcat gccgcgttcc tcgaggtact cgcgggaggc acgcgcttcg
     3361 tttcgtgcat ggtcgaactg cccgagcatg gcgcgcagcg ccgctgtgaa gactgcacgc
     3421 ccgaccttcc cggtatcacc cacccacgtg atcgcctcga tgcgcgtgat cgcctcggcg
     3481 gccggaacgg ggccccagcg caggctgttg cagatcatca cgttgcaccg cagccagtgt
     3541 cggggatcct cgtcagggtc gagcagctcg cgtgctcgct cgagcgcggc aagctcatcc
     3601 atccatcggc actccatgag gtaggcatca gcgcgagcga actccgcggc gatccttccg
     3661 gacctctccc cccgctcaac cgccagaccc tcgacccggc tcgccaggct caacatctgc
     3721 gattggtcca tcgatgggtc gatcagctgc cgcgcctgcc ccatccattc gagctctgct
     3781 cgcatctcga gaaggtcgtc ctgcgctcgt cgggcagctg ccttgacctc gtcaaacatt
     3841 gccgtggctc gcgggaagtc cccttcgtcg acggccgcgg cggccaggtg catcaggagc
     3901 tcaccgcgct ggcgagggtc ctcgagcagc gccacagcgc gcgagagcag gttcgtggtg
     3961 gccggtggat cgttgcgagc aagcgcccgc aggcctgcgg atcgcaggtg cgatgcagcg
     4021 ctggcggcaa gagagcgggt cagctcatcg gtcaatccca gctcagctcg gtaccgatga
     4081 gcctgttcga ggtgatacgc gatcacctct tcgtactcga gctgccgctc gccggcgacc
     4141 ccttccagcc aggcggcgaa gcgggcgtgg agatcggccc gctgctcctt gggaagggag
     4201 ccgtaggcgg cgtcccggac caggatgtgt cggaagcgga aggcctcgtc gccggttgtg
     4261 ccggaacgat cggggcgcac cagctccttg cggaccaggg tcatgagccg cgatccaagc
     4321 tcctccctcc gctccggcgg cgacagctcg gtgacggccg agcgctggaa gaccttgccg
     4381 accaccgatg ctcgcccgat caggtcgcgt tccgacggct cgagccggtc cagccgcgca
     4441 gccacgagag ccgacaccgt gggcgggacg ctgatctggc tcaggtctgc atctgactgc
     4501 cagctcccgt tcacccgccg cagcacgccc tcgtcgacca gcatcgccac cagctcctcg
     4561 acgaagaggg gattcccttc ggcggtctcg ccaatccgac cctgcacggc acgggcgagc
     4621 gtctcgtcgt tgatcaggtt gccgaccagc tcggccgaat catctgcggt gagtggctcg
     4681 agcaggatcg tcgtggcgtt catctttccg ccgccccact gcggtcgcac atcgagcatc
     4741 tcgggccggg cgacggccag caggaggatc ggggcctcgt gcgaccagtc ggcgatgtgc
     4801 tcgacgaggt cgagcatggt tggctccgcc cagtgcaggt cgtcgatgac cacgatcagt
     4861 ggctgcggcc gggcgagcgt ctcgagcaat ttccgaacgc cccagaagat ctcctcggac
     4921 ggtatggcgg actcggagag accgattgcg gcggcaatcc gctccacgat ggcatcccgc
     4981 tcgtctgcgc cctcgagcag cgtggtgagc ttttcacggg cggtctccgc tccatcggcg
     5041 gggttgatcc cggcggcggc ctgcacgagc tcgacgatcg gccagaaggt gatgccttcg
     5101 ccgtacggca ggcaacgggc gcgaaggacc cgcgcgccac cagcgacctg ctgaaggaat
     5161 tcgtggatca gccgcgactt gccgaccccc gccgtgccca gcagcgtgaa gaggtggcat
     5221 gcctcttcgc gcttcgctcg ttcgaacgcc tcgcccagga ttcgcagctc gctctggcga
     5281 ccgacgagtg gcgtgtcatg gcgccggatc ggctgctgct ccttgaggcc caggaggcga
     5341 taggcctcga cgggctcagc cttgcccttc agctcgagcg cggggatcgg ctcggcttcg
     5401 atcgcgtcac gaaccagccg gtatgtgcct gcacccacga tcacctcgcc cgggttggct
     5461 gcctgctcga ggcgggccgc cacgttcacc gcatcgccga tgacgagcgc ctcccccgca
     5521 accgggtcac cggccaccac ctccccagtg ttgaccccc
//
LOCUS       DATCQR010000030         3152 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_202964, whole genome shotgun sequence.
ACCESSION   DATCQR010000030 DATCQR010000000
VERSION     DATCQR010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3152
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202964"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(330..1379)
                     /locus_tag="VIN32_01055"
     CDS             complement(330..1379)
                     /locus_tag="VIN32_01055"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HEY8921194.1"
                     /translation="MPPILRRADDTDRPAWDAFITSRPEADLLQTWAWGECTAMAGEP
                     PARLLLDDNGQIRGVAQALMRPAGFGRQVAYVAHGPIWDRSAPDADRLFAWLLQGLRT
                     LARKEKALVLKLDPRAEPGDATELAGLARSNGLRPAPDLQAPTTRIVDLLDGGDQLEA
                     SWHADARRLSRRAEREGTTLTISRDPDPAQIAILHELLTLTAERGDFRVRSPEFLECL
                     ATELKRSNGWYLGIARVEDTPIAAMAFPRIGNRAYYLYGALRRDPEYKHNYGAHAVMA
                     SMLRALAADGCATVDMWGVVEPDNATADPAWRGFSDFKRTFGGLPLRHPGLFDLVTDL
                     FWYRVRSAWERLPGR"
     gene            complement(1391..1927)
                     /locus_tag="VIN32_01060"
     CDS             complement(1391..1927)
                     /locus_tag="VIN32_01060"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEY8921195.1"
                     /translation="MTSERRRPLVVDASVGVAICIEEPAVDPALSILRERDAHGGKLL
                     VPAFFWLEVVNVLVRRYRQPPGLVVASIVDLQNLNLETVELDRPQLLLTIDAMGRGAL
                     SAYDAAYLALAESADAELLTFDRRLAVAAGDRAIPMDGGHTIWEARESYAPSWAGWPG
                     AAAYLKKLRAQVAGQATG"
     gene            complement(1917..2213)
                     /locus_tag="VIN32_01065"
     CDS             complement(1917..2213)
                     /locus_tag="VIN32_01065"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HEY8921196.1"
                     /translation="MRTVTAMALRARLGEILDAASAGERIVIERDHRPMAVLIPYEDA
                     ARLEPDEQERIARSLEALDRLVARGKWFRASGLMPADAPDAVTAIREERARDDI"
     gene            complement(2254..>3152)
                     /locus_tag="VIN32_01070"
     CDS             complement(2254..>3152)
                     /locus_tag="VIN32_01070"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HEY8921197.1"
                     /translation="GAQVLIRTVPLVGWRLGYAPRGPIGQFDEPETRRSMVAAMRHLG
                     VLEGIATLKVDPQAIAGDPLGSTLLGPPWRASAKVQPPRTRVIDLSLDEDALLAGMRK
                     KHRQYVHKAERARVRIERLAMNAPPNEIATALADFYAIYRETAERAGFAVRVPEYYER
                     VWRLFAPGGHASLFFATLEGARVAALFHFTCGDRAAEAYGGMTDAGAASHANYLLKWE
                     AIRSFKAAGFASYDLWGIATGGIAQFKEGFGGRQIEYIGARDLPLRPVQDAALRLLLP
                     AYGIAQRARLRLTGHKLAGSDE"
BASE COUNT          512 a         1020 c         1133 g          487 t
ORIGIN      
        1 cgcctctact tcttcccgcc tcaacttccc ggcccagcgg ctaccctccg ccaagtctcg
       61 tcgcagcctg gttcacgact agacttcgac gatggaccga aatccgactg ggtctctcga
      121 gcagggcgcc atgctatccg cgcggaaacc gggggatgtc ctgggtcgaa gcgggaggag
      181 cggcggctct ctggcagtct tgcttgtcgt gctcgtgatc atcggagcct ggatcctgct
      241 cagtggcaat cagcgcggcc catcgccgag tccctcgcct agtccatcgg cctcggtctc
      301 gatctagcgc acgcgctggg cgtcatggct cagcgccccg ggagtcgctc ccatgccgag
      361 cgtacgcggt accagaacag atccgtgacg aggtcgaaga ggccggggtg gcggagcggg
      421 aggccgccga aggtgcgctt gaagtcggag aagccgcgcc aggcaggatc ggcggttgca
      481 ttgtccggct cgacgacgcc ccacatgtcc acggtcgcgc atccatcggc ggcgagcgcc
      541 ctgagcatgg aggccatcac cgcgtgggcg ccgtagttgt gcttgtactc ggggtcgcgg
      601 cggagggcac cgtacaagta gtaggcgcga ttgccgatgc gagggaaggc catggcggcg
      661 atcggcgtgt cctcgacgcg agcgatgccg aggtaccagc cgttcgagcg cttcagttcg
      721 gtcgccaggc attcgaggaa ctcgggggag cgaacgcgga agtcgccgcg ctcggcggtc
      781 agggtcagga gctcatggag gatcgcgatc tgcgcgggat cggggtcgcg agagatggtg
      841 agcgtcgtgc cttcgcgctc ggcgcgacgc gagaggcggc gcgcgtcggc gtgccatgag
      901 gcctccaatt gatccccgcc gtccagcaga tcgacgatgc gcgtggtcgg ggcctggagg
      961 tcgggtgcgg gacggagccc gttgctcctc gcgagaccag cgagttcggt cgcgtcaccg
     1021 ggctcggcac gcgggtccag cttgaggacg agcgccttct ccttgcgagc gagggtgcgc
     1081 aggccctgga gcagccaggc gaagaggcgg tcggcatcag gagccgatcg gtcccagatc
     1141 ggtccatgcg cgacgtacgc gacctggcgg ccgaacccgg cgggacgcat taacgcctgg
     1201 gcgacaccgc ggatctggcc attgtcgtcg agcagaagac gggcaggtgg ctcgccggcc
     1261 atggcggtgc actcgcccca tgcccaggtt tgaaggagat ccgcctccgg gcgggaggtg
     1321 atgaaggcgt cccaggcggg tcggtcggtg tcgtcggcgc ggcggaggat gggaggcatc
     1381 gggcagaagc ctacccggtg gcctggcctg ccacctgtgc acggagcttc ttcagatagg
     1441 cggcggcgcc cggccatccg gcccacgacg gggcgtagga ctcgcgagcc tcccagatgg
     1501 tgtgtccacc atccatcggg atggcgcggt caccggcagc cacggcaagg cggcgatcga
     1561 acgtcaacag ctcggcatcc gcagattcgg ctagagccag gtaggcggcg tcgtatgccg
     1621 acaaggcacc gcggcccatg gcgtcaatcg tcagaagcag ctgggggcgg tcgagttcga
     1681 ctgtctccag attgaggttc tgcaggtcga cgatcgacgc gacaaccagc cctggcggct
     1741 ggcggtagcg cctgaccaga acgttgacca cctcgagcca gaagaatgcc gggaccagaa
     1801 gcttgccgcc gtgtgcgtcg cgctcccgaa ggatcgacaa ggccggatcg acggccggct
     1861 cctcgatgca gatcgcaacg ccgaccgagg cgtcaaccac gagtggacgc cgtctttcag
     1921 atgtcatcgc gcgctcgctc ctcgcggatg gcggtgaccg catcgggcgc gtctgccggc
     1981 atcagacccg aggctctgaa ccacttcccg cgagcgacca gacggtcgag cgcctcgagg
     2041 ctgcgcgcga tgcgctcttg ctcatccggc tcgaggcggg cagcgtcctc ataggggatt
     2101 aaaacggcca tcggcctgtg gtcgcgctcg atcacgatgc gctcgcccgc tgacgccgcg
     2161 tccaggatct cgcccaaacg cgctcgaagt gccatggctg tcaccgttct catggtcaac
     2221 catgatgatc gactatgaga atgacgtcaa gggtcattcg tcgctgccgg cgagcttgtg
     2281 gccggtgagc cgcaggcgcg ctcgctgggc gatgccgtag gcgggcagca gcaggcggag
     2341 tgccgcgtcc tggaccgggc ggagaggaag gtcccgggcg ccgatgtact cgatctgcct
     2401 gccgccgaag ccctccttga actgggcgat gcccccggtc gcaatgcccc acaggtcgta
     2461 gcttgcgaag ccggcggcct tgaacgatcg gatcgcctcc catttgagca ggtagttggc
     2521 atgggacgcc gcgccggcat cggtcatgcc gccatacgcc tccgcggcgc ggtccccgca
     2581 ggtgaagtgg aagagcgcgg cgacacgcgc cccttcaagg gtcgcgaaga agagactggc
     2641 gtgaccgccg ggggcgaaga ggcgccagac ccgttcgtag tactcgggca cccggacggc
     2701 gaacccggcg cgctcggcgg tctcccggta gatcgcatag aagtcggcca gggcggtggc
     2761 aatctcattg ggcggggcat tcatggcgag ccgctcgatc cgcacccgcg cccgctctgc
     2821 cttgtggacg tactgccggt gcttcttccg catgccggcc aggagcgcgt cctcgtcgag
     2881 cgacaggtcg atgacccggg tccgaggcgg ctgcaccttg gccgacgccc gccagggcgg
     2941 gccaaggagc gtcgagccga gcgggtcgcc agcgatcgcc tgggggtcca ccttgagcgt
     3001 ggcgatcccc tcgaggacac ccaagtggcg catggccgcg accatggatc ggcgcgtctc
     3061 cggctcgtca aattggccga ttgggccgcg aggagcgtag ccgaggcgcc aaccaaccag
     3121 cgggacggtg cggatcagca cctgggcacc gg
//
LOCUS       DATCQR010000031         6713 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_156371, whole genome shotgun sequence.
ACCESSION   DATCQR010000031 DATCQR010000000
VERSION     DATCQR010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6713
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156371"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..604)
                     /locus_tag="VIN32_01075"
     CDS             complement(<1..604)
                     /locus_tag="VIN32_01075"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY8921198.1"
                     /translation="MRFALMSEPQQGLTYDEILAMAHTAEAAGLEAFFRSDHYGSFGG
                     SADGPTTDAWATLAGLARDTSTIRIGSLVSPITFRLPGSFAKVVATVDEMSGGRVEVG
                     MGAGWNEEEHRELGIPFPALRERYDRLEESLAIVHGLWTEPDSWSFKGQHWQVRNAHL
                     RPRPTFSGKRHPHLVLGGDGGPRLARLVATYADEFNRQSAT"
     gene            complement(652..3162)
                     /locus_tag="VIN32_01080"
     CDS             complement(652..3162)
                     /locus_tag="VIN32_01080"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY8921199.1"
                     /translation="MLGSALVVSALVLPRMSAIDPAWQLPWWVMAPVFWAAEAQVIHF
                     HFRRGAHTFSLNELPLVVGFFLANPLELVIAQAIGSAAALAINRRQPPVKLAFNLGSF
                     ALSSVIGLMVFRAVGAGGLTEPMRWLAAFLAVSISNLVGVTTVASAISISEGRLGVPK
                     LRQMIAVSSVVAITNTSIALLAVIIIVADSRAVWLLTLPIATLFLGYRAFIGAKQQHE
                     SLELLYESTRILQRTPELDSALVQLLYHSRTMFRAEQAEIVLFPAAPGDSYLITSVAE
                     GRTSVMAEASATAWRPVHQHVVEHGTAFLLNTTSIELEDDRDVRDAMIAPLGTDDGVI
                     GAMVIANRLGDVSTFTAEDLRLFATVANHAGTALENGQLERSLAKLSVLQDELKHQAF
                     HDGLTQLANRGLFGESVNARLAATPRGKRSVVMFVDLDDFKFVNDMYGHPAGDAMLVE
                     VAARLQACTRTSDLTARLGGDEFAILVDDDPSLVNAERVAGRIAQILQEPFSIGEVRT
                     HVSASIGIAAGSRGDSAEDLLRQADVAMYSAKAAGKGRWVVYKPSMLKTVRSRNDVGA
                     ELQLAVDRGEFQLRYQPVVSLESGRIVGAEALVRWNHPTRGEVGPDEFIGNAEENAMI
                     VPIGRWVLAEACREAAGWSQQAGGTTPWVAANLSARQLLHPTLLDDVSTALAASGLAP
                     SSLVLEVTETVLLHDADAAIEVLGKLRARGIQIALDDFGTGYSSLSYLHRFPIDILKI
                     AQGFVDVDDAKDDRWILAQAIISLGKALGLRVIAEGVERRAQVQRLQSAGCEFGQGFF
                     FARPLHGDRLGAEFARDNAQRLGSDRMARSIGIESNAA"
     gene            complement(3391..4836)
                     /locus_tag="VIN32_01085"
     CDS             complement(3391..4836)
                     /locus_tag="VIN32_01085"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEY8921200.1"
                     /translation="MPRRLAVALGSLAVVASAIAPAASMAAAYDPAGDMNSMFNTTQY
                     TGAQAYWNAGLTGAGVDVAIIDTGVTAVDGLATPGKLVYGPDLSFESQTDALRNLDTN
                     GHGTFMAGIIAGRATGAVAGSYVGDSANFLGVAPDARILSVKVGVADGGVDVSQVIAA
                     IDWVVQHRTDNGMNIRVLNLSYGTDSLQSYQADPLAFAVEQAWKAGIFVVTATGNAGY
                     AFKTGTLTNPAYDPKIFAVGSSESMGTATQGDDTVSAYSSTGSNGRTPDVVAPGAHIV
                     SLRVPESYIDQNYGSTGYVTPTLFRGSGTSEAAAFVSGAAALVIQQHPTITPASLKRL
                     FLANATGLGTSSLRQGAGEIDLSRVLVATVYAPPTQWKTIDWSTGFGSLELSRGTDHL
                     TRDGVILSGSIDIFGHAFESVPMAILEAAGNSWSGGTWNGNSWSGNSWSGNSWSGNSW
                     SGNSWSGNSWSGNSWSGNSWSSNGWMGVSWH"
     regulatory      complement(5052..5135)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(5175..5765)
                     /locus_tag="VIN32_01090"
     CDS             complement(5175..5765)
                     /locus_tag="VIN32_01090"
                     /inference="COORDINATES: protein motif:HMM:NF014668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HEY8921201.1"
                     /translation="MAGRRRRRIPDAERPAPDPEAAMEIAVRFLGTRPRTRWELERRL
                     RRASAPDDVITATLDRLATMGYVDDTAFARWWAEQRDRHAPRGRRMVEAELRQHGVPR
                     DVLESLRGEELAEPMLDADSVPGGEAERARTALHRHLKGRLMPLDLKARQRIGAFLMR
                     RGFDPETVRSTIRKVAAESTPGGSTAEPDGLGDEGP"
     gene            complement(5781..>6713)
                     /gene="recA"
                     /locus_tag="VIN32_01095"
     CDS             complement(5781..>6713)
                     /gene="recA"
                     /locus_tag="VIN32_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014138431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEY8921202.1"
                     /translation="VSRQQVDFIPTGSIALDLALGVGGIPRGRITEIFGPESSGKTTL
                     CYHIAANAQRAGGIVAFIDAEHALDPTYAKNVGLNVDELLVSQPDTGEQALEIAETLI
                     RSNGVDVVIIDSVAALVPRAEIEGEMGDSFMGLQARLMSQALRKLTGAINRSNTALIF
                     TNQLREKIGVMFGNPETTPGGRALKFYCSVRLDIRRIETLKNGTDAIGSRTRVKVVKN
                     KVASPFRLAEFDIMYNEGISREGGLLDVGIAAGILSKTGAWFNYGETRLGQGRENSRD
                     FLKAHPELADRVESEIRGKVATIEVGVEGIPEAE"
BASE COUNT         1072 a         2211 c         2302 g         1128 t
ORIGIN      
        1 gcgttgccga ctgtcggttg aactcgtcag cgtaggtggc gaccagccgg gccagccggg
       61 gccctccatc cccgcccagg accaggtggg gatgtcgctt gccggagaag gtcggtcggg
      121 gccgcaggtg ggcgttgcgt acctgccaat gctgtccttt gaagctccag ctgtcgggct
      181 cggtccacag gccgtgcacg atcgccagcg actcctccag gcggtcatac cgctcgcgca
      241 gggcgggaaa tgggatgccc agctcgcgat gctcctcctc gttccagccg gcgcccatcc
      301 ccacctcgac gcgcccgccg ctcatctcgt ccacggtggc gacgaccttg gcaaagctgc
      361 cggggaggcg gaaggtgatc ggcgagacga gggagccgat gcggatcgtg gaggtgtcgc
      421 gagcgaggcc ggcgagcgtg gcccaggcat ccgtggtcgg tccatcggcc gagccgccga
      481 aggacccgta gtgatccgac cggaagaagg cctccaggcc ggcagcctcg gcggtgtgcg
      541 ccatggccag gatctcgtcg taggtcaacc cctgctgggg ttcgctcatg agggcgaatc
      601 gcatggccgc agactagcgt gccgcaggca cgctcgggga tgccgatttc cctaggcggc
      661 gttgctctcg atgccgatcg agcgggccat ccggtcggag ccgagccgct gcgcgttgtc
      721 tcgcgcgaat tccgcaccga gccgatcgcc gtgcagcggg cgggcgaaga agaatccctg
      781 gccgaactcg catcccgcgc tctgcagccg ctggacctgc gcgcgccgct cgaccccctc
      841 cgcgatcacc cgcaggccga gcgcctttcc gagcgagatg atcgcctggg ccaggatcca
      901 gcgatcgtcc ttggcgtcat cgacgtcgac gaacccctgc gcgatcttga ggatgtcgat
      961 cgggaagcga tgcaggtagc tgagcgagga gtagccggtc ccgaagtcgt ccagcgcgat
     1021 ctggatgcca cgggcgcgga gcttgccgag gacctcgatg gcggcgtccg cgtcatgcag
     1081 caggaccgtc tcggtcacct ccagcacgag gctggatggt gccaggccgg aagccgcgag
     1141 cgcggtgctg acatcgtcga gcagggtcgg gtgcaggagc tggcgagccg acaggttggc
     1201 tgcgacccaa ggtgtggtgc cgcctgcctg ctggctccag ccggccgcct cccggcatgc
     1261 ctcggccagc acccatcggc cgatcggaac gatcattgcg ttctcctcgg cattgccgat
     1321 gaactcgtcg ggcccgacct cgccgcgagt cgggtggttc cagcgcacga gtgcctcggc
     1381 acccacgatc cggccggatt cgagcgatac gaccggctgg tagcgaagct ggaattcccc
     1441 ccgatcgacc gcgagctgca gctcggcgcc gacgtcgttc cgcgatcgga ccgtcttgag
     1501 cattgatggc ttgtagacga cccagcggcc cttgcccgcc gccttggcgc tgtacatcgc
     1561 cacgtccgcc tggcggagta ggtcctcggc cgagtcgccc cggctgccgg ccgcgatgcc
     1621 gatgctggcc gacacgtggg ttcgaacctc accgatcgag aagggctcct ggaggatctg
     1681 tgcaatgcgg ccggccaccc gctccgcgtt gacgaggctc ggatcgtcgt cgaccaggat
     1741 ggcgaactcg tcgccgccca atcgagcggt caggtccgat gtccgcgtgc atgcctgcag
     1801 ccgagcggcg acctccacca gcatcgcgtc ccctgccgga tggccgtaca tgtcgttcac
     1861 gaacttgaag tcgtcgaggt cgacgaacat cacgactgat cgcttccctc gcggcgtcgc
     1921 ggccagccgg gcattgacgc tttcgccgaa gagtccccgg ttggccagct gtgtgagccc
     1981 gtcgtggaat gcctggtgct tgagctcgtc ctgcagcacg gagagcttgg ccagcgagcg
     2041 ctccagctgc ccgttctcca gggcggtgcc ggcgtgattc gccacggtgg caaacaagcg
     2101 aaggtcctcg gccgtgaagg tgctgacgtc tccgagccgg ttggcgatca ccatggcgcc
     2161 gatgactccg tcgtcggtcc cgaggggcgc gatcatcgca tcgcgcacgt cgcggtcgtc
     2221 ctccagctcg atggacgtcg tgttgagcag gaacgccgtc ccgtgctcca cgacgtgctg
     2281 gtggacaggc ctccaggcgg tcgcggacgc ctcggccatc accgaggtcc tgccttccgc
     2341 caccgacgtg atcaggtagc tgtcgccagg tgccgccggg aagaggacga tctcggcctg
     2401 ctcggctcga aacatggtcc ggctgtggta cagcagctga acgagcgccg agtcgagctc
     2461 gggggtccgc tgcaggatgc gggtcgactc gtacagcagc tccagcgact catgctgctg
     2521 cttggcgccg atgaaggcgc ggtacccgag gaacagggtt gcgatcggca gggtcaggag
     2581 ccagaccgcg cgcgaatcgg cgacgatgat gatgaccgcc aggagggcga tgctggtgtt
     2641 ggtgatggcc acgaccgacg atacggcgat catctggcga agcttcggga ccccgagccg
     2701 gccctccgag atggagatgg cggaagcgac cgttgtgacc ccgaccaggt tcgagatgct
     2761 cactgccagg aaggcggcaa gccatcgcat cggctccgtc aagccgcccg ccccgaccgc
     2821 gcggaagacc atcagtccga tcaccgatga cagcgcaaac gatccgaggt tgaaggcgag
     2881 ctttaccggc ggctgacgcc ggttgatggc gagggccgcc gccgagccga tcgcctgcgc
     2941 gattaccagc tccagcgggt tcgccaggaa gaagccgacg acgagcggca gctcattcag
     3001 agagaacgtg tgcgcgcccc tgcgaaagtg aaagtggatc acctgggcct cggctgccca
     3061 gaagacggga gccatgaccc accacggcag ctgccaggcc gggtcgatgg ccgacatgcg
     3121 aggcagcacc agggccgaca ccaccagcgc ggagccgagc atgacggcga cgagggtcca
     3181 caccctcgct ggtggtcgca tggcctaggt cacccggccg gtcatcggat tgagcctacc
     3241 ttcgtgtgct tgtaatgccg agccgccgat cgtcccgtgt tggtggggac gaatgaggga
     3301 ttggtgctca gctctgccct tttcctggtg aaaatggtgt gcggtcgagg tacgaccccg
     3361 accgcacgac gttgtggcaa ctccggcggg ctagtgccag gagacgccca tccagccatt
     3421 gctggaccag ctgttgccgg accacgagtt cccgctccag ctgttgccgg accacgagtt
     3481 gccggaccac gagttcccgc tccacgagtt gccggaccac gagttgccgg accacgagtt
     3541 gccgttccag gttccgccgc tccagctgtt gccggcagcc tcgaggatgg ccatcggaac
     3601 ggactcgaaa gcgtgtccga agatgtcgat cgatccgctc aggatcaccc cgtcgcgagt
     3661 gaggtgatcg gttccgcgcg agagctcgag cgagccgaac ccggtcgacc agtcgatcgt
     3721 cttccactgg gtcggagggg cgtagacggt cgcgacgagc accctggaca ggtcgatctc
     3781 acccgctccc tgcctcaggc ttgaggttcc gagacctgtc gcattggcga ggaacagccg
     3841 cttgagcgag gcgggggtga tcgtcgggtg ctgttggatg acgagcgcgg ctgcgccaga
     3901 cacgaacgca gcagcctcac ttgtgccgct cccgcggaac agcgtggggg tgacgtagcc
     3961 ggttgagccg tagttctggt cgatatacga ctccggcact cgaaggctga cgatatgcgc
     4021 gcccggggca acgacgtccg gggttcgccc gttgctgccg gtcgacgaat aagcggagac
     4081 ggtgtcgtcg ccctgcgttg ctgttcccat cgactccgac gagccgacgg cgaagatctt
     4141 cgggtcgtag gccgggttgg tcagcgtacc ggtcttgaag gcataaccgg cgttgccagt
     4201 tgcggtgacc acgaagatgc cggccttcca cgcctgttcg acggcaaacg cgagggggtc
     4261 ggcctggtac gactgcaggc tgtccgtgcc ataggacagg ttgaggacac ggatgttcat
     4321 gccgttgtcg gtgcgatgct gaaccaccca gtcgatggcc gcgatcacct gggagacgtc
     4381 gacgccccca tcggcgacgc cgaccttcac cgagaggata cgggcgtcag gagcgacgcc
     4441 gaggaagttg gcgctgtcac cgacgtagct gcctgccacc gcgccagtgg cccggccggc
     4501 aatgatgcca gccatgaagg tgccgtggcc gttcgtgtcg agatttcgaa gcgcatcggt
     4561 ctgcgactcg aaggagaggt cggggccgta cacgagcttc cctggtgttg cgagcccgtc
     4621 gaccgcggta accccggtgt cgatgatggc gacgtcaacg ccagcgccgg taaggccggc
     4681 attccagtag gcctgggcgc cggtgtactg agtcgtgttg aacatcgagt tcatgtcgcc
     4741 ggccgggtcg taggcggcgg ccatgctggc ggccggggca atcgcagaag cgacaaccgc
     4801 aaggctgccg agggcgaccg ccaggcgacg cggcataagg gacgaatgag tgagatgtgg
     4861 ttgcggcggc gacttctggt aaccccacga aacgccaagc aggatcccca ggcgttggat
     4921 caggccgcgg tcagccgccg aggacatggc aagggcgcgg tcgtcatcac ggccaggtcg
     4981 gccggacgat cccacgccaa cgtcgctgag tgcctgcatc gagtcccctt tcgttggggt
     5041 gatgcgcggc tactggcggc tgggccatct cggcttgttg gccgagaccc ctggctttgc
     5101 gcccccacct cgcgatgggt ttgccctttt caggccgtcg cctgtgacaa cgcgtctcgt
     5161 tgcctgcggc gaccttaggg accttcgtcc cccaggccat ctggctcagc agtactcccc
     5221 ccgggggtac tttcggcggc gaccttccgg atggtgctgc gcaccgtctc ggggtcgaag
     5281 ccgcgccgca tcagaaaggc gccgatacgt tgacgagcct tcaggtcgag aggcatcaga
     5341 cgccccttca ggtgacggtg cagggcggtt cgcgctcgct cggcctcgcc gcccggcacc
     5401 gaatcggcat cgagcatcgg ctcggccagc tcctcgccgc ggagcgactc gaggacgtcg
     5461 cgcgggacgc catgctggcg cagctccgcc tcgaccatgc gccgtccgcg tggcgcgtgg
     5521 cggtcgcgct gctcggccca ccaccgagcg aatgcggtgt cgtccacata gcccatcgtc
     5581 gccagacgat cgagggtggc cgtgatcacg tcatccgggg cagaggctcg tcgaaggcgc
     5641 cgctccagct cccatcgcgt gcgcggacgc gtgccaagga agcggacggc gatctccatc
     5701 gccgcctcgg ggtctggcgc gggccgctcc gcgtccggga tgcgccgccg ccggcgtccg
     5761 gccatcgctg cggcgcgctc ctactctgcc tcgggaattc cctcgacgcc gacctcgatg
     5821 gtcgccacct tgccgcggat ctccgattcc acccggtcgg caagctcggg gtgcgccttc
     5881 aggaagtcgc gcgagttctc tcggccctgg cccagccgcg tctcgccgta gttgaaccat
     5941 gccccggtct tcgagaggat gcccgctgcg atgcccacgt ccagcaggcc gccctcgcgg
     6001 ctgattccct cgttgtacat gatgtcgaac tcggccagcc ggaacgggct cgcgaccttg
     6061 ttcttgacca ccttcacgcg ggtgcgggac ccgatcgcat cggtcccgtt cttgagggtc
     6121 tcgatgcggc ggatgtcgag gcgcacggag cagtagaact tgagggcacg gccaccaggc
     6181 gtggtctcgg gattgccgaa catgaccccg atcttttccc gcagctggtt tgtgaagatc
     6241 agcgcggtat tgctgcgatt gatggccccg gtcagcttac ggagcgcttg gctcatcagc
     6301 cgagcctgga gtcccatgaa gctgtccccc atctcacctt cgatctcggc gcgtggcacg
     6361 agcgccgcca ccgagtcgat gatgacgacg tcaaccccat tgctgcggat cagggtctcc
     6421 gcgatctcga gtgcctgctc gccggtatcg ggctgggaga cgagcagctc gtcgacgttg
     6481 agcccgacgt tcttggcata ggtgggatcg agcgcatgct cggcgtcgat gaaggccacg
     6541 atcccgccgg ctcgctgcgc attggcggcg atgtggtagc agagggtcgt cttgccgctt
     6601 gactcgggcc cgaagatctc cgtgatccgg ccgcgcggga tgccgccgac accgagcgcg
     6661 aggtccagcg cgattgaccc ggtcgggatg aagtcgacct gctgtcggga gac
//
LOCUS       DATCQR010000032        10021 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_94363, whole genome shotgun sequence.
ACCESSION   DATCQR010000032 DATCQR010000000
VERSION     DATCQR010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 10021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10021
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94363"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..518)
                     /locus_tag="VIN32_01100"
     CDS             complement(<1..518)
                     /locus_tag="VIN32_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008481986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEY8921203.1"
                     /translation="MDSDRIDAVRRVSIEDEMRSAYIDYAMSVIVARALPDVRDGLKP
                     VHRRILYTMHEMGLRSTSAYRKCAGVVGDALAKYHPHGDVALYDALVRMAQDFSLRYP
                     LVDGQGNFGSVDGDPAAAYRYTEARLTAISDEMLADIEKETVDFVDNYDGRLREPTVL
                     PARLPNLLINGSS"
     gene            complement(565..1101)
                     /locus_tag="VIN32_01105"
     CDS             complement(565..1101)
                     /locus_tag="VIN32_01105"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEY8921204.1"
                     /translation="MSTASGKAATIGSSRLIEAADGRRYAFADHNCFACGGTNPIGMH
                     LAIELGEGTASTTWVPGPNYVGWEEKVHGGLLATLLDEVMAWAPSSFDSWAVTAEISV
                     RYRSPANPGEALTARGWVTQRRRRIYQVSGEVRGADGRLVAEGHGRFLGASPSVKAEL
                     KARYGMPTDVTAAGARRE"
     gene            complement(1098..1346)
                     /locus_tag="VIN32_01110"
     CDS             complement(1098..1346)
                     /locus_tag="VIN32_01110"
                     /inference="COORDINATES: protein motif:HMM:NF025482.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4287 domain-containing protein"
                     /protein_id="HEY8921205.1"
                     /translation="MPSRHVADIRQVEGKTGRKLEDWCALLEANGVGERREAVNFLRD
                     GYAVSHAFALAIAAHCLRPSRTDEFGADGFWDDEEDRW"
     gene            complement(1367..3346)
                     /gene="gyrB"
                     /locus_tag="VIN32_01115"
     CDS             complement(1367..3346)
                     /gene="gyrB"
                     /locus_tag="VIN32_01115"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEY8921206.1"
                     /translation="MTAQAERKPRRTKVGSDYTAENIQVLEGLSAVRKRPSMYIGSTD
                     SRGLHQLVWEIVDNSIDEAMANVATRIDVTIHADGQLEVVDDGRGIPVGRHRSGKDAL
                     EVVHTVLHAGGKFGGGGYKVSGGLHGVGVSVVNALSVAMRVEVLRDGKRYVQEYSRGA
                     PKGSVKQQGGAAEANRGLVTSKERQHGTRTFFQADPEIFEDLDFSWDLIATRLRESAY
                     LNKGLWICLRDERADREKNFYFEGGVTSFVRHLNRRRDVLNQRPIHVERMVGTTSVEV
                     ALQYNDGFAENVLAFANNIHTVDGGTHVTGFRAALTSSLNDWAHKAGVLSEKDANLSG
                     EDVREGLTAVISVKLTDPQFEGQTKAKLGNADVKGAVQAAVTDGIIQHLEENPADGRR
                     IIEKSVTAARAREAARKARDLVIRKGALDGMALPGKLADCQERDPARSELYIVEGDSA
                     GGSAKQGRDRRTQAVLPLRGKLLNVEKARLDRVLSSENIRPLIIALGAGIGDQFDISK
                     LRYHKICLLSDADVDGAHISTLLLTFFFRHMPEVIDRGYLYLCQPPLYRVSTGKVTRY
                     AKDEKSRDQAIKDLSREGKTKNISVQRFKGLGEMNPEQLWETTMNPDTRTLLQISVRE
                     AADADEVFSTLMGERVEPRRDFIRGEARKVRNLDF"
     gene            complement(3343..4200)
                     /locus_tag="VIN32_01120"
     CDS             complement(3343..4200)
                     /locus_tag="VIN32_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEY8921207.1"
                     /translation="MAKTFGLGRGLDALIPRAAPTSDVPEIPIDRIARNPHQPRNRFD
                     DAEMADLAASIGLHGVLQPVVVRATADGGYELIAGERRLRAARLAGLTHIPAVVRESA
                     DGEQLELALVENLQRRDLNAIEEAAAYRELIDRFALSHEEVARRVGKSRVAISNALRL
                     LDLTPEAREAIADGRISEGHGRALAALTVPELQRAALLIVIERHLSVRQTEELVRRRR
                     DSGPTQRGGQISGDLADLEAQLRELLATRVGIVRTRRGGRLVIDFYSDEELDRLHGII
                     ARGVGGVGA"
     gene            complement(4193..4954)
                     /locus_tag="VIN32_01125"
     CDS             complement(4193..4954)
                     /locus_tag="VIN32_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY8921208.1"
                     /translation="MVRVTACTNQKGGVGKTTTVVNLAAYLALSGTSTLVIDLDPQGN
                     ATSGLGVDRRSLDRSVYDALINRVPIGELVVETSVPGLDLVPAALALSGAEVELVGVP
                     AREHRLSASLADLGQRYERVLIDCPPSLGLLTVNALTAADGVLIPIQTEYYALEGLSQ
                     LVNTIRMVREALNPRLEIDGVLLTMYDGRTRLSVQVAAEVRRHLEGAVYDTVVPRNIR
                     LSEAPSHGVPIAIYDPTSRGAEAYGRLAAEVTARG"
     gene            5170..5667
                     /locus_tag="VIN32_01130"
     CDS             5170..5667
                     /locus_tag="VIN32_01130"
                     /inference="COORDINATES: protein motif:HMM:NF016019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921209.1"
                     /translation="MSYVSLLLAVVVGILHGAVAPILVIGDVHPNLVLVAVVLVTVLN
                     GFGPGVAWAFVGGLTANLLIRQPLGSLPLALLLVAAAVAGGERLFGRLSWGYPLVSVL
                     IGSVVVDVISLGLLQMVDVSLAGGFPIPRVIAAALLNTAIAAIVLLPTRMLLSRAGAA
                     ETGAW"
     gene            5675..7579
                     /gene="mrdA"
                     /locus_tag="VIN32_01135"
     CDS             5675..7579
                     /gene="mrdA"
                     /locus_tag="VIN32_01135"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEY8921210.1"
                     /translation="MSDLEGTPTARPSRARFLAATIIAVVLFGILAGRLFQLQVVDGA
                     LYAAQAAAARTIEMPIRAPRGLIFDREGRPVAVNIPSWTLYARPADLPATKSAREAAL
                     AQAAGLAGIGVSTILNRLAAFRGSPFELVPLATDIGRNAALLIAEQQEQLPGISVGAE
                     PRREYLDETGARNGELLAHLVGYTGPISPTEFDDRSAVGYLRDDVIGRDGVEASYENA
                     LRGTYGSELLERDAAGRPVKVLQRTTDPVPGKNLMLTIDARTQRIATETLKWGLEAAG
                     VAQGVTIVMNPQTGEILAMVSLPTYDNDKFASGISAPDYAAYLDDPTRPLRNHAIADI
                     YPPGSTFKLVTGLAALDEGVTTVPQLWPTYGCYQIPNAPAGQCLFDWNHTGFGPLSIV
                     DAYAKSSDTFFYQMAVALGVDRLGGWAEQLGFGEKSGVQLPSEAAGTVVSKAWAQAQG
                     RQDVFTGELAQAGIGQNAIAVTPLQLLNAYAAVANGGHLMRPMIVRGETDAEGKLTRA
                     YAPELIRDLAASPADLRTMRIGAREVITSGHATNINALQVPGALSGKTGTAEFGTPTS
                     QGVLPFHSWFVAYLPSKAGATDAQLAIVTFTYSAVVRGNVSLEVIKYFLQEYFNLDQD
                     LRREFQVNAN"
     gene            7583..8719
                     /gene="rodA"
                     /locus_tag="VIN32_01140"
     CDS             7583..8719
                     /gene="rodA"
                     /locus_tag="VIN32_01140"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HEY8921211.1"
                     /translation="MSAATATFVELRSARRWRAFDWQLLLYVVLLVGFGMVMGYSANY
                     QAIGGESGLSQTVKTLIWTSIGLTLFFVAASIDYHWLQTFATPIYLVVLGLLLATVVV
                     GRNLFGAQMSISVAGIDFQFSEISKVLMVVVLAAFLASRRERIGRLSTIVGAGLLMAL
                     PTFLVYRQPDLGTAMVFVAILLGMLFMSGAGIGWLSLLGGATLAVAPIAVGALEEYQR
                     QRLFCFLDPSADPQGACYQVVQALNAVGSGGVLGQGLTAGRQNQLGFLPVQSTDFIFT
                     VVAEELGFIGGMILLALFALLIWRILSIGWGARDLFGAGIAAGLASLLLFQITVNIGM
                     VTGIMPVTGIPLPFITYGGSSMISLLFGMGLLQSVRVHSSKPTF"
     gene            8744..9457
                     /locus_tag="VIN32_01145"
     CDS             8744..9457
                     /locus_tag="VIN32_01145"
                     /inference="COORDINATES: protein motif:HMM:TIGR03936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HEY8921212.1"
                     /translation="MATRNSDQPRQRWQILFARRHPALRIRQAELMSEFERAFREAGL
                     PLATSNAQHPRPRLKFAANLPVGIELRGEVLDAYFDDLVPLDRIQAAAELLPEGIEVV
                     DAREVWHGFPSAASQLRAAEYEVEVHDGPELTSDALRGAIVRLLAAPQLPGMRRRGES
                     ERRADAGPRDLRPLLEDAEVLEVDEEAHSGRLRLVLRLDASGAGRPEDVVGALDLPVR
                     AGPAVRTRLLFVDTPPIAR"
     gene            9514..>10021
                     /gene="rplU"
                     /locus_tag="VIN32_01150"
     CDS             9514..>10021
                     /gene="rplU"
                     /locus_tag="VIN32_01150"
                     /inference="COORDINATES: protein motif:HMM:NF013025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEY8921213.1"
                     /translation="MAPRLERIHFAAPEMIMYAVLSSGGKQYRVEAGTELMLERLDGV
                     PGEAGAQVSFDRVLLIGDGDDVTIGTPTVAGASVSATILGEALGPKLVIFKFKQKVKY
                     RRRTGHRQHMTRVRIDTINPTAAKRETAKAKADASEAVAGAPAKAEKKAAPRTTRPRT
                     KKAAAAEPK"
BASE COUNT         1510 a         3388 c         3377 g         1746 t
ORIGIN      
        1 gagctcccgt tgatgagcag gttgggcagg cgagcgggca gcaccgtcgg ctctcgcagc
       61 cggccgtcgt agttgtcgac gaagtcgacg gtctccttct cgatgtcggc cagcatctcg
      121 tcgctgatcg cggtcaggcg cgcctcggtg tagcggtagg cggcggctgg atccccgtcg
      181 acggagccga agttgccctg cccgtcgacc aacgggtacc gaaggctgaa gtcttgcgcc
      241 atccgcacca gggcgtcata gagcgccacg tcgccgtgcg ggtggtactt ggcgagggcg
      301 tcacccacca cgccggcgca cttgcgatag gccgatgtgg agcggagtcc catctcgtgc
      361 atcgtgtaca ggattcggcg atgcacgggc ttgagcccgt cccgcacgtc gggcagcgct
      421 cgcgcgacga tcacgctcat cgcgtagtcg atgtacgccg atcgcatctc gtcctcgatc
      481 gagacgcggc gcacggcgtc gattcggtct gaatccatcg gtccggctct tactcctgca
      541 cgcttgtcgc gtttgtcgtc gtgttcattc ccgtcgagcc ccggccgctg tgacgtcggt
      601 cggcatcccg taccgggcct tgagctcggc cttgacggaa gggctcgctc ccaggaaccg
      661 gccgtggccc tcggcgacga ggcggccgtc cgcccctcgg acctcgccac tgacctggta
      721 gatgcgcctt cgccgctggg tcacccagcc acgcgccgtg agcgcctcgc ccgggttcgc
      781 cggagaccga tatcggaccg agatctcggc cgtcaccgcc cacgagtcga agctcgatgg
      841 cgcccaggcc atcacctcgt cgagcagggt ggccagcagc cctccgtgca ccttctcctc
      901 ccaaccgacg tagttcgggc caggcaccca ggtcgtgctg gccgtcccct cgcccagctc
      961 gatcgccagg tgcatgccga tcgggttcgt gccgccgcag gcgaagcagt tgtggtccgc
     1021 gaacgcgtag cgccggccgt cggcggcctc gatcagccgg ctgctgccga ttgtggccgc
     1081 cttgccgctg gcggtgctca ccagcggtct tcctcgtcgt cccagaagcc atcggcgccg
     1141 aactcgtcgg ttcgcgatgg gcgaagacaa tgcgcggcga tggcgagcgc aaaggcgtgg
     1201 ctgaccgcat atccgtcgcg caggaagttg acggcctcgc gccgctcccc cacgccgttc
     1261 gcctccagca gggcgcacca gtcctcgagc ttgcggccgg tctttccctc gacctggcga
     1321 atgtcggcaa cgtgtcggct tggcatggtt ctggcatcgg ctccgtctag aagtccaggt
     1381 tgcggacctt gcgcgcttca ccgcgaatga agtcgcggcg aggctcgaca cgctcgccca
     1441 tgagcgtgct gaacacctcg tcggcgtcgg ccgcctctcg cacgctgatc tgcagcaggg
     1501 tgcgcgtgtc cgggttcatg gtggtctccc acagctgctc gggattcatc tcgccgagcc
     1561 ccttgaagcg ctggacgctg atgttcttgg tcttcccttc gcgtgacagg tccttgatcg
     1621 cctggtcgcg cgacttctcg tccttggcat agcgggtcac ctttccggtg ctgacgcggt
     1681 agaggggcgg ctggcagagg tacaggtatc ccctatcgat cacttcgggc atgtgccgga
     1741 agaaaaaggt gagcaggagc gtgctgatgt gcgcgccgtc gacgtccgcg tccgagagga
     1801 ggcagatctt gtggtatcgg agcttggaga tgtcgaactg gtcgccgatg ccggcgccga
     1861 gcgcgatgat caggggacgg atgttctcgc tggacaggac ccgatccagg cgtgccttct
     1921 cgacgttcag cagcttgccg cgcagcggca ggacggcctg cgttcggcgg tcacgaccct
     1981 gcttggcaga gccaccggcg gagtcgccct cgacgatgta cagctcgctc cgcgcgggat
     2041 ctcgctcctg gcagtcagcc agcttgccgg ggagcgccat cccgtcaagg gcccccttgc
     2101 gaatcacgag atcacgggcc ttgcgagccg cctcccgcgc acgcgccgcg gtcacggact
     2161 tctcgatgat gcggcgaccg tcggcggggt tctcctcgag gtgctggatg atgccgtcgg
     2221 tgacggcggc ctggacggcg cccttcacgt cggcgtttcc gagcttggcc ttggtctggc
     2281 cctcgaactg cgggtccgtg agcttgacgc tgataacagc ggtgagtccc tcgcgcacgt
     2341 cctcgccgct caggttggca tccttctccg acaggacccc ggccttgtgc gcccagtcgt
     2401 tgagcgacga ggtgagcgcg gcccggaagc cagtgacgtg cgttcccccg tcgaccgtat
     2461 ggatgttgtt ggcgaaggcc agcacgttct cggcgaagcc atcgttgtac tggagcgcga
     2521 cctcgacgct ggtggtgccc accatccgct caacgtggat cggtcgctgg ttgagcacgt
     2581 cccggcgccg gttcaggtgc cggacgaagc tcgtgacgcc accctcgaag tagaagttct
     2641 tctcgcgatc ggctcgttcg tcgcgcaggc agatccagag tcccttgttc aggtaggccg
     2701 attcccgcag ccgcgtggcg atcaggtccc agctgaagtc gagatcctcg aagatctccg
     2761 gatcggcctg gaagaaggtg cgcgtgccgt gctgtcgctc cttgctcgtg acgagaccgc
     2821 gattggcctc ggccgcaccg ccctgctgct tgacggagcc cttgggcgcg ccccggctgt
     2881 actcctgcac gtagcgcttg ccatctcgga ggacctcgac ccgcatggcg accgagaggg
     2941 cgttgacgac gctcacgccg accccgtgca ggccgcctga gaccttgtat ccgccgccgc
     3001 cgaacttgcc gccagcgtgc agcacggtgt ggacgacctc gagcgcgtcc ttcccggatc
     3061 gatgcctgcc gaccgggatg ccgcgcccgt catcgacgac ctcgagctgc ccgtcggcgt
     3121 ggatcgtgac gtcgatccgc gtggcgacgt tggccatcgc ctcgtcgatg ctgttgtcga
     3181 cgatctccca gacgagctgg tgcaggccgc gagagtcggt ggagccgatg tacatgctcg
     3241 gccgcttgcg gaccgcactc aggccctcca gcacctggat gttttcggcc gtgtagtcgg
     3301 atcccacctt ggtgcgccgc ggcttgcgct ccgcctgcgc cgtcatgcgc caactccccc
     3361 gacgccacgg gcgatgatgc cgtgcaggcg atccagctcc tcgtcggagt agaagtcgat
     3421 cacaaggcgc cctccgcgac gtgtccggac gatcccgacc ctggtggcca gcagctcgcg
     3481 cagctgcgcc tcgagatcgg cgaggtcacc cgagatctgg ccgccccgct gggtcggccc
     3541 tgagtcacgc ctgcgccgca cgagctcctc ggtctggcgc acggaaaggt ggcgctcgat
     3601 gacgatcagg agggccgctc gttggagctc gggcaccgtg agcgcggcga gcgctcgacc
     3661 gtgaccctcg gagatgcgtc cgtcggcgat cgcttctcgg gcctctggag tcaggtcgag
     3721 caggcggagg gcgttgctga tggcgacgcg gctcttcccg accctgcggg ccacctcctc
     3781 gtgcgagagg gcgaagcggt cgatcagctc gcgatatgcc gcggcctcct cgatcgcgtt
     3841 gaggtcccgc cgttgcaggt tctcgaccaa cgccagctca agctgctctc cgtcggccga
     3901 ctcgcgcacc acggcgggga tgtgggtgag gcccgcgagg cgtgcagcgc gcagtcggcg
     3961 ctcgccggcg atcagctcat aacctccatc ggcggtcgcg cgcacgacga ccggctggag
     4021 cacgccgtgg aggccgatcg acgcggcgag atccgccatc tccgcatcgt cgaaccggtt
     4081 gcggggctgg tgcgggttgc gagcaatccg atcgatcgga atctcgggca cgtcggaggt
     4141 cggagcggcg cgtggaatga gcgcatccag tccgcgaccc aggccgaagg tcttagccac
     4201 gtgcggtcac ctcggccgcc aggcgtccgt aagcctcggc accacgtgac gtggggtcat
     4261 agatggctat cggcacgccg tggctgggcg cctcggagag ccgaatgttg cgcggcacca
     4321 cggtgtcgta caccgcccct tccaggtggc gacgcacttc cgccgcgacc tggacggata
     4381 gcctcgtacg accgtcgtac atagtcagca gcactccgtc gatctcgagc cgcgggttga
     4441 gcgcctcgcg aaccatgcgg atcgtattca ccaattggct caatccctca agcgcgtagt
     4501 actccgtctg gatcgggatc agtaccccgt ccgccgccgt caatgcgttc accgtcagca
     4561 gcccgagcga tggtgggcag tcgatgagca ctcgttcata tcgttgaccc aggtctgcca
     4621 ggctcgccga gagccgatgt tcgcgcgccg gcaccccgac cagctcgacc tccgcgcccg
     4681 acagggccag cgcggctggc accaggtcga gccccgggac cgaagtttcg accaccagtt
     4741 cgccgatcgg gacccggttg atgagagcgt cgtacacgga ccgatccagc gaccttcggt
     4801 cgacccccag gccgctcgtc gcgttgccct gcgggtcgag gtcgatgacc agggtcgatg
     4861 ttccggagag ggccagatac gccgccaggt tgacgacggt cgtcgtcttg ccaacgccgc
     4921 ccttctggtt ggtgcaggcc gtgaccctga ccacgggcac tccgctgcga gttcgaaatg
     4981 ggctgcctta ttctaccagt cgaggccctt actgaaggct tatcgcgacg ttacgatccg
     5041 tcagaagttt cacgtgaaac aggcgcagca atcctcgtga aggccagggg catcggtata
     5101 cttccggcac cgcccattgc agccccactc gcccccgagc cggtcccgtg agccgttcaa
     5161 ggaacgcaga tcagctacgt cagtcttctc ctcgcagtag tggtcgggat cctgcacggc
     5221 gctgtggcgc ccatcctcgt gatcggcgac gttcatccga atctcgtgct ggtggccgtc
     5281 gtcctggtaa ccgtgctcaa tggcttcggg ccgggcgtgg cgtgggcctt cgtggggggc
     5341 ctgacagcaa acctcctcat tcgccagccg ctcggcagcc tccctctcgc cctattgctg
     5401 gtggcagccg cggttgccgg cggcgaacgc ctcttcggcc ggctgtcgtg gggataccca
     5461 ctcgtctcgg ttctgatcgg gtccgtggtg gtcgacgtga tcagcctcgg gctcctgcag
     5521 atggtggatg tctcccttgc cggcgggttt cccatcccga gggtcatcgc tgccgcgctg
     5581 ctgaacaccg cgatcgccgc gatcgtcctc ctgcccacgc gaatgctgct gtcgcgagcg
     5641 ggcgcggccg agaccggagc ctggtgagcg ccagatgtcc gacctagagg gcacccccac
     5701 cgctcggcct tcacgtgcac gattcctggc cgcaaccatc atcgccgtgg tcctgttcgg
     5761 catcctcgcc ggccgcctgt tccagctcca ggtggtggac ggcgcgctgt acgcggcaca
     5821 ggcagcggcc gcgcgaacca tcgagatgcc aattcgcgcc ccgcgcggcc tcatattcga
     5881 ccgcgaaggc cggccggttg ccgtcaacat cccctcctgg accctgtatg cgcgcccggc
     5941 cgacctgccc gcgacgaaat ccgcccggga agccgcactc gcgcaggccg ccggcctggc
     6001 tgggatagga gtgagcacga ttctgaatcg gctggcggcg ttccgcgggt cgccattcga
     6061 gctcgtcccg ctcgccaccg atatcggccg caacgccgca ttgctgatag cggagcaaca
     6121 ggagcagctg ccagggatca gcgtgggagc cgagccgagg cgcgaatacc tcgacgagac
     6181 cggggcgcgg aacggcgagc tgctcgctca cctggtcggc tacaccggcc ccatctcacc
     6241 cactgagttc gacgaccgtt cggccgtcgg ctacctgcgg gacgacgtca tcggccgtga
     6301 cggcgtagag gcgagctacg agaatgccct ccgcggcacc tacggcagtg agctgctcga
     6361 gcgggatgca gccgggcggc cggtgaaggt gctccagcgc accactgacc ccgtgccggg
     6421 caagaacctg atgctgacga tcgacgccag gacgcagcgc attgcgaccg agacgctcaa
     6481 gtgggggttg gaggccgccg gggtcgcgca gggggtgacg atcgtgatga atccccagac
     6541 tggcgagatc ctggcgatgg tctcgctgcc gacctacgac aacgacaagt ttgcctcggg
     6601 gatctcggca ccagactatg ccgcctacct tgacgatccc accaggccgc tgcgcaacca
     6661 cgcgatcgcc gacatctacc cgcccgggtc gaccttcaag ttggtgaccg gcctggcggc
     6721 gctcgacgag ggagtcacca cggtcccgca gctttggcca acctatggct gctaccagat
     6781 cccgaacgcc ccggccgggc agtgtctctt cgactggaac cacaccgggt tcggtcccct
     6841 gagcatcgtc gacgcctacg ccaagagctc ggacaccttc ttctaccaga tggccgtggc
     6901 gctcggggtc gatcggctcg gagggtgggc ggagcagctc ggcttcggcg agaagagcgg
     6961 agtgcagctc cccagcgagg ccgccggcac agtcgtcagc aaggcgtggg cccaggcgca
     7021 ggggcgccag gacgtcttca ccggcgagct ggcacaggcg ggaatcggcc agaacgccat
     7081 cgccgtcacc ccgctccagc tcctgaacgc ctacgccgcg gtcgcgaatg gcggacatct
     7141 gatgcggccg atgatcgtcc gcggcgagac ggacgcagag ggcaagctca cccgcgccta
     7201 cgcaccggag ctgatccggg acctggccgc aagccccgcg gacctgcgca ccatgcgcat
     7261 cggcgctcgc gaggtgatca ccagtggaca cgccaccaac atcaatgccc tgcaggtccc
     7321 gggtgcgctg tcggggaaga ccggaaccgc cgagttcggg accccgacca gccagggggt
     7381 cctccccttc cactcgtggt tcgtggccta cctgccatcg aaggcgggag cgacggacgc
     7441 gcagctcgcg atcgtgacct tcacctattc ggcggttgtt cgcgggaacg tctcactgga
     7501 ggtgatcaag tacttcctcc aggagtactt caacctcgac caggatctgc ggcgagagtt
     7561 ccaggtcaat gcgaactgac cgatgagcgc agcgacagca acctttgttg agttacgcag
     7621 tgctcgccgc tggcgggcct tcgactggca gctgctgctg tacgtcgtcc tgctggtcgg
     7681 gttcgggatg gtgatgggct attcggccaa ctaccaggct atcggcggcg agagcggcct
     7741 ctcgcagacg gtcaagacgc tcatctggac gagcatcggc ctcaccctgt tcttcgttgc
     7801 ggccagcatc gactaccact ggctgcagac cttcgctacg ccgatctacc tggtcgtgct
     7861 cggcctcctg ctggccaccg tggtggttgg caggaacctc ttcggggcgc agatgtcgat
     7921 cagcgtggcc ggcatcgact tccagttcag cgagatcagc aaggtgctga tggtcgtggt
     7981 gctggcggcc tttctcgcat cacgacgcga acggatcgga cgcctgagca ccatcgtcgg
     8041 tgcggggctg ctgatggcgc tgccgacgtt cctggtctat cgacagcccg acctcgggac
     8101 ggccatggtg ttcgtggcga tcctgctcgg gatgcttttc atgagtggcg ccgggatcgg
     8161 ctggctctcg ctgctcggcg gggcgaccct cgcggtggcg ccgatcgccg tgggcgcgct
     8221 ggaggaatac cagcggcagc ggctcttctg cttcctggac ccctcggccg atccgcaggg
     8281 tgcctgctac caggtggtgc aggcgctgaa tgccgtgggc tcgggtggtg tgctggggca
     8341 gggcttgacg gccggccgcc agaaccagct cggcttcctg ccggtgcagt ccaccgactt
     8401 catcttcacc gtggttgccg aggagctcgg cttcatcggt ggaatgatcc tgctggccct
     8461 cttcgccctg ctgatctggc gcatcctgag catcggttgg ggagcgcggg atctgttcgg
     8521 ggcgggcatc gccgccggtc tcgccagcct gctcctgttc cagatcacgg tgaacatcgg
     8581 gatggtgacg gggatcatgc cggtgaccgg catcccgctc ccgttcatca cgtacggcgg
     8641 ttcctcgatg atctcgctgc tgttcgggat ggggctgctg cagtcggtcc gggtgcactc
     8701 ctcgaagccg accttctgac cgcgcggtac catcgttgga ccgatggcca ccagaaacag
     8761 cgaccagcca cgtcagcgct ggcagatcct cttcgccagg cgtcaccctg cgctgcggat
     8821 tcggcaggcg gagctgatga gcgagttcga gcgcgcgttc cgcgaggctg gcctgccgct
     8881 ggcgacgtcg aacgcgcagc atccgcgacc gaggctgaag ttcgcagcca acctgccggt
     8941 cgggatcgag ctgcgggggg aggtattgga cgcctacttc gacgatctcg tcccgctcga
     9001 tcgcatccag gcggccgccg agctgctgcc cgaggggatc gaggtggtcg acgcacgtga
     9061 ggtttggcat ggctttccgt cggcggcctc ccagctgcgg gcagctgagt acgaggtcga
     9121 ggtgcacgac gggccggagc tgaccagcga tgcactgcgc ggagcgatcg tcaggctcct
     9181 ggccgcgccg cagctgcccg gcatgcggcg acgaggagag agcgagcggc gggccgatgc
     9241 cgggcctcgc gaccttcgcc cgctcctgga ggacgccgag gtgctggagg tggacgagga
     9301 ggcgcattcg ggaaggctgc ggctcgtgct gcggctcgat gccagcgggg ccgggaggcc
     9361 ggaggacgtc gtgggagcgc tcgacctgcc ggtgcgagcg ggtccggcgg tccggacgcg
     9421 gctcctgttc gttgacactc ccccgatcgc ccggtagact cgtcgtcccg cgggcgcatc
     9481 gtctggcggc ttcgccgtgc gtcggttcgg aacgtggccc ctcggttgga acgcatccat
     9541 ttcgcagctc cggagatgat catgtacgcc gtcctcagca gcggcggaaa gcaatatcgg
     9601 gtcgaggccg gcaccgagct catgctcgag cgccttgacg gtgtacctgg cgaggccggt
     9661 gcgcaggtgt ccttcgatcg cgtgctgctc atcggcgacg gcgacgacgt caccatcggc
     9721 acccccaccg tggccggcgc ctcggtgagc gccacgatcc tcggcgaggc gctggggccc
     9781 aagttggtca tcttcaagtt caagcagaag gtcaagtatc gacgccggac agggcaccgc
     9841 cagcacatga cccgagtccg catcgatacg atcaacccca cggcggccaa gcgggagact
     9901 gcgaaggcga aggcggatgc gagcgaggcc gtcgccgggg cgccggccaa ggcggagaag
     9961 aaggcggcgc cacgcaccac caggccgagg acgaagaagg cagcggctgc cgagccgaag
    10021 g
//
LOCUS       DATCQR010000033         4836 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_110021, whole genome shotgun sequence.
ACCESSION   DATCQR010000033 DATCQR010000000
VERSION     DATCQR010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4836
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110021"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..464)
                     /locus_tag="VIN32_01155"
     CDS             complement(<1..464)
                     /locus_tag="VIN32_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018090635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8921214.1"
                     /translation="MTAPVVAGESGEPVEGRGRRFARWLYPRRRAQLWLLLAAPVLWI
                     GLVYLGSLLILLLNAFWAKDAFTGNVTPFDWTLKAFADILTKPVYAIVAGRTVLIAVL
                     VTITDALLAFPIAYYMARIASPRRRGALVVAVLLPLWAAYLVKTFAWRTILQG"
     gene            complement(554..1723)
                     /locus_tag="VIN32_01160"
     CDS             complement(554..1723)
                     /locus_tag="VIN32_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019876474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY8921215.1"
                     /translation="MRRLSIPAALVTIGIVMAACGSSVPSGAPTSLGDNEKDLNLIIW
                     DGYAERGEVDKAYDWVTGFEADTGCKVHTTPMSDSANGVQLLKSGQYDGGSFSGDATI
                     RLMAAGDVVPVNVDLIPNYADVFAGLKLKDFNSKDGVPYGVPHGRGPNLLMWNTDVVT
                     TAPTTWDPIFEGGADYAGKISVYDSSIYIADAALHLMQKSPDLGITNPYQLNDKQFAA
                     AIALLQKQRDNGAVYWGLLTDQVQSFEAGDTVIGTTWPYQVNLLKAEDPPAPVEATLP
                     TEGSTGWSDTWMIAKNAAHPNCMYRWMNHILSADANAQATVWFGEAAVTQKACDAAEA
                     IAPGHCKDQHASDEAYWDKIWYWTTPQANCADADDATTCKDQDAWVEAWSTLRGT"
     gene            complement(1723..2898)
                     /locus_tag="VIN32_01165"
     CDS             complement(1723..2898)
                     /locus_tag="VIN32_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018556753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8921216.1"
                     /translation="MTASADTQLPGKPNPEPAQRAAAITLEHLVKRFGDDEIGRRVAQ
                     FVVHDGTFPVTAVAGIDLEILDGEFFSMLGPSGSGKTTTLRMIAGFEAPTEGRILLHG
                     KDVTGVPPFDRDVNTVFQDYALFPHMTVGDNVAYGLMVRRIPAGERAARTAEALRMVR
                     LEGYEKRRPAQLSGGQRQRVALARALVNRPRVLLLDEPLGALDLKLREEMQIELKEIQ
                     GQVGITFIYVTHDQEEALVMSDRIAVFNRGLIEQVGTPADVYEHPRTAFVAAFVGTSN
                     LLTAAAARDILGMDGTFTVRPEKIRIGAPEDSVAPDEHSALGRIREVVYLGADTRYIV
                     ALDAGGELIVTQQNLQASSMEALAAQGRAVRLIWQRQHVLALKGSTTTLGEGEGAET"
     gene            3053..3865
                     /locus_tag="VIN32_01170"
     CDS             3053..3865
                     /locus_tag="VIN32_01170"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEY8921217.1"
                     /translation="MGSAAAWGTGDFAGGLAARRAGGMLVTGGAQIVGFILLLVAVAI
                     LRPSLPAPSTLLLGALGGVAGGLGLAALYRGLAMGAMGLVSAVSGVGGVLIPLAIGTL
                     LLGGVIQPLQLAGVGCAVLAIGAASGATTRGVSREALLLALVAALGFGLWFIFLDRAS
                     EHDQLWALVASRGSASLVVGGSALLRADRSRLGAVVPLVALAGLLDVAANGMVVLAFA
                     TIPVGIAAALSGTYPLATMLLARAFLGEALPRLGLLAVALAVIGIVLISLGG"
     gene            4099..>4836
                     /locus_tag="VIN32_01175"
     CDS             4099..>4836
                     /locus_tag="VIN32_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011210111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEY8921218.1"
                     /translation="MTFDRVAIVEASLDAYLAQAAASATVLPPDAPLRQGSGLSARKA
                     VELFEDQLTSRQLDVAARELKKTNRSFYTISSAGHENNVVVGAQLRINDPAFLHYRSG
                     ALMMARARQLPGSTPAFDALLGIVASSDDPIAQGRHKVWGSRPLWVPPQTSTIASHLP
                     KAMGLAFSLARARRLGVATDLPDDAIVLCSFGDATVNHATALAAINTARYAVRLGLPM
                     PILFVCEDNETGISVPTPEGWIASSFGH"
BASE COUNT          687 a         1667 c         1648 g          834 t
ORIGIN      
        1 ccctgcagga tcgttcgcca cgcgaaggtc ttcaccaggt acgccgccca gagaggcagc
       61 agcaccgcca ccaccagtgc cccccgccgg cgaggagagg cgatgcgagc catgtagtac
      121 gcgatcggga aggccagcag ggcgtcagtg atcgtgacca gcaccgcgat caagaccgtt
      181 cgcccggcga cgatggcgta gaccggcttg gtcaggatgt cggcgaatgc cttcagcgtc
      241 caatcgaacg gggtcacatt gccggtgaag gcgtccttgg cccagaacgc gttcagcagc
      301 aggatcagca gcgagccgag atagacgaga ccgatccaga gcacgggtgc cgcgagcagc
      361 agccatagct gcgcgcgtcg tcgcggatag agccagcggg cgaaccgtcg cccgcgaccc
      421 tcgactggct cgccggattc gccggcgacg actggagcgg tcatcgaccc tgcgcgctca
      481 cctcatgacg atgagtctcc ccgggcggcg gacctcccgg ggagacagac tctgcaggct
      541 agctaatcgt ggctcaggta ccccgcaacg tgctccaggc ctcgacccat gcatcctggt
      601 ccttgcatgt cgtcgcgtcg tccgcatcgg cgcagttggc ctgtggggtg gtccagtacc
      661 agatcttgtc ccagtacgcc tcgtcgctgg cgtgctggtc cttgcagtgg cctggggcga
      721 tcgcctcggc cgcgtcacag gccttctgcg tgacggccgc ctctccgaac cacaccgtcg
      781 cctgcgcgtt ggcgtccgcg gagaggatgt ggttcatcca gcgatacatg cagttgggat
      841 gagccgcgtt cttggcgatc atccaggtgt cggaccagcc ggtcgagccc tcagtcggga
      901 gggtcgcctc gaccggggcc ggcgggtcct cggccttcag caggttgacc tggtacggcc
      961 aggtcgtccc gatgacggta tcgcccgcct cgaatgactg cacctggtcg gtcagcaggc
     1021 cccagtacac ggcgccgttg tcgcgctgct tctgcagcag ggcgatggct gccgcgaact
     1081 gcttgtcgtt gagctggtag gggttggtaa tccccaggtc ggggctcttc tgcatcaggt
     1141 gcagggccgc atcggcgatg tagatggacg agtcgtagac gctgatcttg ccggcgtaat
     1201 cggcgccacc ctcgaagatc ggatcccagg tggtgggcgc ggtggtgacg acgtccgtgt
     1261 tccacatcag aaggttcggg ccgcgtccat gcggtacgcc gtacgggacc ccgtccttcg
     1321 agttgaagtc cttgagcttg aggccggcga agacgtccgc gtagttcggg atcagatcga
     1381 cgttgacggg gaccacgtcg cccgccgcca tcagccggat cgtggcgtcg ccggagaacg
     1441 agccgccgtc gtactgcccg ctcttgagca gctgcacgcc gttggccgag tcgctcatcg
     1501 gggtggtgtg aaccttgcag ccggtatcgg cctcgaagcc ggtgacccag tcgtaggcct
     1561 tgtcgacctc gccgcgctcg gcgtacccgt cccagatgat caggttgagg tctttctcgt
     1621 tgtcccccag cgaggtcggg gccccgctcg gcaccgacga gccgcaggcg gccatgacga
     1681 tgccgatggt caccagggct gccggaatcg aaagtctccg catcaagtct ccgctccctc
     1741 tccttccccc aacgtggtcg tgctgccctt gagggccaaa acgtgctggc gctgccagat
     1801 gagccggact gcccgaccct gggcggcgag cgcctccatc gaggacgcct ggaggttctg
     1861 ctgggtcacg atcagctcgc caccggcgtc cagggcgacg atgtagcggg tatcggcgcc
     1921 aaggtacacc acttccctga tccgcccaag ggcggaatgc tcgtccgggg ccaccgaatc
     1981 ttcaggtgcg ccgatgcgga tcttctccgg tcggacggtg aaggtgccat ccattcccag
     2041 gatgtctcgc gccgcagccg ccgtgagcag gttcgacgtt ccgacgaacg ccgccacgaa
     2101 ggcggtccgc gggtgttcat acacatccgc gggcgttccc acctgctcga tcagcccgcg
     2161 gttgaagaca gcgatccggt cgctcatgac cagcgcctct tcctggtcgt gcgtgacgta
     2221 gatgaaggtg atgccgacct ggccctggat ctccttcagc tcgatctgca tctcctcgcg
     2281 gagcttcagg tcgagcgcgc ccaacggctc gtcgagcagc aggacgcgag gccggttgac
     2341 gagtgcgcgg gccagcgcca cccgctgccg ctggcctccc gacagctgcg ccggccgccg
     2401 cttctcgtag ccctccaggc gcaccatgcg aagcgcctcg gcggttcgcg ctgcgcgctc
     2461 gccggcgggg atcctgcgca ccatcagccc gtaggccacg ttgtccccca ccgtcatgtg
     2521 cgggaagagg gcgtagtcct ggaagacggt gttgacatcg cggtcgaagg gcgggacgcc
     2581 cgtcacgtcc ttgccgtgaa gcaagatgcg tccctccgtc ggggcctcga atccggcgat
     2641 catgcgcagg gtcgtggtct tgcccgaccc ggacggaccg agcatcgaga agaactcacc
     2701 gtcgaggatc tccagatcga tgccggcgac cgcggtcacc gggaaggtgc cgtcgtggac
     2761 gacgaactgc gcgacgcgcc gtccgatctc gtcgtcaccg aatcgcttga cgaggtgctc
     2821 gagcgtgatc gcggctgcgc gctgtgctgg ctcggggttg ggtttacccg ggagctgggt
     2881 gtcggcggaa gcggtcatcg gttgaaggcc acgagctccc ttcgctgcgc cggagatttg
     2941 gggcactctg gcggtcccgc ccgatgcatg tcaagcagcc ccgtggctgg ctggtacatt
     3001 cccgggcgac tgaatcggga ggtgtctgat cgaacctggc gtcctgctcg gggtgggatc
     3061 ggccgcggcc tggggcacgg gcgactttgc cggcggcctg gccgctcggc gcgccggcgg
     3121 catgctcgtc accggcgggg cccagatcgt cggcttcatc ctgctgctcg tcgcggtcgc
     3181 gatcctgcgc ccatccctgc ccgccccctc gaccctgctc ctcggggcgc tgggcggcgt
     3241 cgccggcggg ctgggactgg cggccctgta ccgcgggctg gcgatggggg cgatgggatt
     3301 ggtctccgct gtcagcgggg tcggcggagt gctcataccg ctcgccatcg gcaccctgct
     3361 gctcggcggc gtcatccagc cgctgcagct ggcgggagtc ggctgcgccg tgctcgccat
     3421 cggtgcggcg agcggtgcca ccacgcgcgg cgtgagccgc gaagcgttgc tgctggcact
     3481 ggtcgctgcg ctcggcttcg gcctctggtt catattcctc gaccgcgctt ccgagcacga
     3541 ccagctctgg gcgctggttg ccagccgcgg ctccgcgtcg ctggtggtgg gcggcagcgc
     3601 gctgctgcgg gctgaccgat cgcggctcgg cgcggtcgtc ccgctcgtcg ccctcgccgg
     3661 gctgctggac gtggcggcca acgggatggt ggtcctggcc ttcgcgacta ttccggtcgg
     3721 catcgccgcc gccctctcgg gcacctaccc gctggcgacc atgctcctgg cgcgggcctt
     3781 cctgggcgag gcattgccgc ggcttggcct gctggctgtc gccctggccg tcatcggcat
     3841 cgtcttgatt tcgctcggcg gctgatcgag cgcgtcttca gctcggccag ccgcccccgg
     3901 tgtgggcacc tggcgctggc atcaggcctg gcatgcgcgt tgctccccga cggcaaccgc
     3961 cgcgccacga tggaggacac ggatcggtgc cgtgcaagga gacagctgcc gaccttactg
     4021 cccgcttatc agccgcttac tcggcgggta tcatggccgg accccccgcc tccgacacca
     4081 ccgagggccg cacctgcaat gaccttcgac cgcgtcgcca tcgtcgaggc atcgctcgac
     4141 gcatacctcg cgcaggcggc tgcctccgcg acagtgctgc ctccagacgc cccgctgcgc
     4201 cagggaagcg ggctgagcgc gcgcaaggcg gtcgagctgt tcgaggacca gctcacctcg
     4261 cgccagctgg acgtggctgc gcgtgagctg aagaagacga accgcagctt ctacaccatc
     4321 agcagcgccg gccacgagaa caacgtcgtg gtgggcgcgc agctgcgcat caacgacccg
     4381 gccttcctgc actatcggtc cggtgcgctg atgatggcgc gcgctcggca gctgcccggc
     4441 agcacgccgg cgttcgacgc gctgctcggc atcgtcgcca gcagcgacga cccgatcgcg
     4501 cagggccggc acaaggtgtg gggcagccgg cccctgtggg tcccgcctca gacctcgacc
     4561 atcgcgagtc atctccccaa ggcgatgggg ctcgccttca gtcttgccag ggcgcggcgg
     4621 ctcggagttg cgacggacct gcccgatgac gcgatcgtgc tttgctcgtt cggtgacgcc
     4681 accgtcaacc acgccactgc cctggcggcg atcaacaccg cccgctatgc cgtccgactg
     4741 ggcctgccga tgccgatcct tttcgtctgc gaggacaatg agaccggcat cagcgtgccg
     4801 acgcccgagg gctggatcgc ctcgagcttc ggccac
//
LOCUS       DATCQR010000034         4766 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_63853, whole genome shotgun sequence.
ACCESSION   DATCQR010000034 DATCQR010000000
VERSION     DATCQR010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4766
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63853"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..477)
                     /locus_tag="VIN32_01180"
     CDS             complement(<1..477)
                     /locus_tag="VIN32_01180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921219.1"
                     /translation="MKTELIFVLVSGALAALVGLGAFVAVLRGWSERTPIPAPAPNAT
                     DDTLAGTPLATPSHDAVVRVVWWVAIAGVLVGIGISNAFATNRVAIYALGGAAVVIVV
                     VLHEMLPRRLRGRASVAVEVVAALALATGLVWLTGGANSPFVFPFDLLVVAAALTMG"
     gene            complement(713..789)
                     /locus_tag="VIN32_01185"
     tRNA            complement(713..789)
                     /locus_tag="VIN32_01185"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(753..755),aa:Pro,seq:tgg)
     gene            complement(831..1970)
                     /gene="mazG"
                     /locus_tag="VIN32_01190"
     CDS             complement(831..1970)
                     /gene="mazG"
                     /locus_tag="VIN32_01190"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HEY8921220.1"
                     /translation="MRSRGATTSRGIDGLLAAAGLDPALGVQVVSGRRLASIAFDPSL
                     PLVLLAEPAAREAPSVLPGRHARKGSREVLLSLYPHDHPVQSLPDGMPRFLESLSDEE
                     LCGADWLVPALSALDNLASPHGMAAISARLRAPDGCPWDRKQTHATLRPFLLEEAYET
                     VDAIEGGAPEDLAEELGDLFLQIILHAQLAAEEGAFDLTDVYRRLGAKIIRRHPHVFG
                     DVEVSGAEEVLRNWEAIKAIERQDAGGPPSAFDGIARALPALAASREIQERASSLGWD
                     WPAIDGVWEKVDEELAELHEAEAIEGDAGRDARLHEFGDVLFAAVNLARWLKLDPEEA
                     LRSANRRWIERYERVEALASERGLVLIELSPDAKDALWNEVKARG"
     gene            complement(1967..3691)
                     /locus_tag="VIN32_01195"
     CDS             complement(1967..3691)
                     /locus_tag="VIN32_01195"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY8921221.1"
                     /translation="MSFRNRPVLDRKHRPRWQDELRTQQLIVAGFAIAIAVAIGIFGA
                     AAWSDHYEAHLRPVAAVGDVTYTLDDLTDRMDAISAELQARYEDLQGRLGGVRDSLIQ
                     QALQSIQDALSALAPTAADSLVLGRVLSSSAGRYGISVSDEAVSAEVTKRQTVPERLK
                     LSIITVAALPKDAASGAKATDADWARAEKEINAIASAIKGGADFAATAKEKSGDSSAA
                     AGGSLSWIEADDPLYSTYFADAEKAAKGDLVGPIKGDTGYRLVRVDDRKAAGTNQRLK
                     DLLAAAGVSDAEYRAYVRGDLLKSAFDDHFMTKVMVPYQPQREVAQIFIAAQKGIPVP
                     QQRVRHFLAQPLPGATDQSKATDAQWAAALARAEAFRVAASKPHADWYVLAKQSDDTG
                     SGSRGGDLGWYDPGSSTFVPEFKAAIAKLKVDQLSEPVKTQFGYHVIEVVAERITAAV
                     QADQMVTTLRAHPDQFAQVARDQSEDADSAAKGGDLGWVIPYQFEKERVDAIFKLTKP
                     GEISDPITTPTGIYIFKLLATAELRWVPTQQLTSVRQTGFDQWLSEIRNRAVTWTDPQ
                     FVAAPSSG"
     gene            complement(3796..4605)
                     /locus_tag="VIN32_01200"
     CDS             complement(3796..4605)
                     /locus_tag="VIN32_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015350714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HEY8921222.1"
                     /translation="MSGETSTPGPVASQPAEPPHVGLAAGRGVTVVIPTYNERENIEG
                     LVDAILEALPEATLLVVDDNSPDGTGSLADTMAAREPRLNVLHRAGKEGLGVALRDGF
                     RWALARGETKAVVQMDADFSHDPRDLPRLLAPLMSGSDLALGTRYMPGGGTIGWPLRR
                     KLISRAGTLFARVMLLLPYRDLTGGFKAWRRELLEGIRLREADASGYGFQIETTWWAH
                     RRRAVVTEIPIIFRERVAGSSKMSGGIVGEALALVLRLRLEAIRDTFRRTK"
     gene            complement(4605..>4766)
                     /locus_tag="VIN32_01205"
     CDS             complement(4605..>4766)
                     /locus_tag="VIN32_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HEY8921223.1"
                     /translation="RRGAGGFGYDPIFMLPSGITTAELPEGEKDLISHRGQAVAAALP
                     RLRALTAVA"
BASE COUNT          741 a         1648 c         1611 g          766 t
ORIGIN      
        1 ccccatggtc agcgccgcag ccacgaccag caggtcgaag gggaagacga agggcgagtt
       61 ggcgccgccg gtcagccaga ccaggccggt ggccagggcg agtgcggcga ctacctccac
      121 ggccacgctt gcgcggcccc gtagccgacg cggcagcatc tcgtgcagga cgaccacgat
      181 caccacggcg gcgccgccga gggcatagat cgccactcgg ttcgtcgcaa acgcgtttga
      241 gatgccgatt ccgaccagca ctcccgcgat cgcgacccac cacaccacgc gcacgacggc
      301 atcgtgcgag ggagtcgcca ggggcgttcc agccagcgtg tcgtccgttg cgttcggtgc
      361 gggggccggg ataggagtcc gctcactcca tcctcggaga accgccacga acgccccaag
      421 accgacgagc gcggccagtg caccgctgac aaggacgaag atcagctcgg ttttcacatt
      481 cctcactcag ggcttccggg ccgtcggaag cgcctgcaat tgccgctgcc cgcctgctgg
      541 ctagtctagg ggcttgctcg agatatgcgc aaaggtacta accccacctg aaccaccttc
      601 cgtggcattc gggcaccgcc tagtcgcaag gtaccgggca cgcaatgcag tcgccttcag
      661 cgtttgagca tcgcgcgatg ccgctgaacg cggcgcacgt gacccagaag gttggtcggg
      721 gcggcccgat tcgaacgggc gacctcctgc tcccaaagca ggtgcgctac caggctgcgc
      781 tacgccccgc gggttggttg ctgagactac agcatcgcgc tcgcgctacg tcatcccctg
      841 gccttgacct cgttccagag cgcatccttg gcgtcgggtg agagttcgat caggaccagg
      901 ccgcgctccg atgcgagcgc ctcgacccgc tcatagcgct cgatccagcg gcggttggcg
      961 ctccgcaagg cctcttcagg atcgagcttc agccagcgcg ccaggttgac cgccgcgaac
     1021 aggacgtcgc cgaactcatg gaggcgggcg tcacggccgg catcgccctc gatcgcttcg
     1081 gcctcgtgca gctcggccag ctcctcatcg accttctccc agaccccatc gatcgccggc
     1141 caatcccagc cgagcgatga ggcgcgctcc tggatctcgc ggctagcggc gagggccggg
     1201 agggcgcggg cgatgccgtc gaacgccgag ggcgggccgc ccgcgtcctg gcgctcgatc
     1261 gccttgatcg cctcccagtt gcggagcacc tcctccgccc cgctgacctc gacgtcgccg
     1321 aacacgtgcg ggtgccgtcg aatgatcttg gcgccgagcc tgcgatagac atcggtcagg
     1381 tcgaaggccc cctcctcggc ggccagctgg gcatgcagga tgatctgcag gaagaggtcg
     1441 cccagctcct ccgcgaggtc ctcgggtgct ccaccctcga tggcgtcgac ggtctcgtac
     1501 gcctcctcca gcaggaatgg gcggagagtg gcgtgcgtct gcttgcgatc ccacggacag
     1561 ccgtcgggtg cccgtagccg agccgagatg gccgccatgc cgtgcggcga ggcgagattg
     1621 tcgagcgcgc tgagggcggg caccagccag tcggcgccgc acagctcctc gtcgctgagg
     1681 ctctccagga accggggcat tccatccggc agtgactgta cggggtggtc atgcgggtac
     1741 agggaaagca gcacctcccg cgaccccttg cgcgcgtgcc gccccggcag gacagagggc
     1801 gcttcgcggg cggcgggctc ggccaggagg acgagcggga gggatggatc gaacgcgatg
     1861 gaggccagtc gccgaccgga cacgacctga acgccgagtg ccggatcgag tcccgccgcc
     1921 gcgaggaggc cgtcgatgcc gcgggacgtc gtggcgcccc gggatctcat ccggagctgg
     1981 gcgcagcgac gaactggggg tcggtccagg tcacggcgcg attgcgaatc tcgctgagcc
     2041 attggtcgaa cccggtctgt cgcaccgatg tgagctgctg ggtcggcacc cagcgcagct
     2101 ccgccgtcgc cagcagcttg aagatgtaga tgccggtcgg cgtcgtgatc gggtcgctga
     2161 tctcgcctgg cttggtgagc ttgaagatcg cgtccacccg ctccttctcg aactggtacg
     2221 ggatgaccca gccaaggtcg ccacccttgg ccgcgctgtc ggcatcttcg ctctggtccc
     2281 tggcgacctg ggcgaactgg tcgggatgcg cccgcagggt ggtcaccatc tgatcggcct
     2341 ggaccgccgc ggtgatgcgc tcagccacga cctcgatgac gtgatacccg aactgcgtct
     2401 tgacgggctc gctgagctga tcgaccttga gcttggcgat cgcagccttg aactccggca
     2461 cgaaggtcga agagccgggg tcgtaccatc ccaggtcgcc gccgcggctt ccactcccgg
     2521 tgtcgtcgct ctgcttggcg agtacgtacc agtccgcatg gggcttgctc gccgccacgc
     2581 ggaaggcctc ggcgcgagcc agtgctgccg cccattgcgc atcggtcgcc tttgactggt
     2641 cggtcgcgcc cgggagcggc tgggcaagga agtgcctgac ccgctgctgg ggcaccggga
     2701 tccccttctg tgcggcaatg aagatctgcg ccacctcgcg ctggggctgg tagggaacca
     2761 tgaccttggt catgaagtgg tcgtcgaagg ctgacttcag caggtcaccg cgaacgtagg
     2821 cgcggtactc ggcatcgctc acccctgcgg cagcgaggag atccttcagg cgctggttgg
     2881 tgcctgccgc cttcctgtcg tcaacgcgca cgagccgata cccggtgtcg cccttgatcg
     2941 ggccgaccag atcgcccttg gcagccttct cggcatcggc gaagtaggtg ctgtacagcg
     3001 ggtcgtcggc ctcgatccag ctgagcgaac ctccggccgc ggccgaggag tcgccgctct
     3061 tctccttggc cgtcgccgcg aagtccgctc cacccttgat ggcgctggcg atggcgttga
     3121 tctccttctc ggcgcgcgcc cagtcggcgt cggtggcctt ggcgccggac gcagcgtcct
     3181 tcggaagggc agccaccgtg atgatcgaca gcttcagtcg ctcaggaacc gtctggcgct
     3241 tcgtcacctc ggcgctgacc gcctcgtcgc tcaccgagat gccgtagcga cccgccgagc
     3301 tgctcagcac tcgcccgagc acgagcgaat cggcggcggt gggagcaagg gcggagagcg
     3361 cgtcctggat cgactgcagc gcctgctgga tgagggagtc gcggacgcct cccagccgtc
     3421 cctgcaggtc ctcgtagcga gcctgcagct cggcgctgat tgcgtccatc cgatcggtga
     3481 ggtcgtcgag ggtgtacgtc acatcgccga cggcggcgac cggtcgcagg tgggcctcgt
     3541 agtggtcgct ccaggcggca gccccgaaga tcccgatcgc caccgcgatg gcgatcgcga
     3601 atccggcaac gatcagctgc tgggtccgca gctcgtcctg ccagcgtggg cggtgcttgc
     3661 ggtcaaggac cgggcggttg cgaaacgaca tctagcgcgt ggttctccgc agggcgatag
     3721 agggacgtct ccgacagtgg gcgaggcgcc atgctagccg atgggccctg cacgtgccag
     3781 cacctcgacc gggcctcatt tggttcgtcg gaaagtgtcc cggatcgcct ccagccggag
     3841 gcgcaggacg agcgccaatg cctcaccgac gatcccgcca ctcatcttcg agctgcccgc
     3901 cacccgctcg cggaagatga tcgggatctc cgtcaccaca gcccgccgcc gatgggccca
     3961 ccacgtcgtc tcgatctgga agccgtatcc gctggcatct gcctcgcgaa ggcgaattcc
     4021 ctcaagcaat tcgcgtcgcc aggccttgaa gccgcccgtc aggtcccgat acggcagcag
     4081 gagcatgacg cgggcgaaga gggtcccggc tcgactgatc agcttgcggc ggagcggcca
     4141 gccaatggtt ccgccgccgg gcatgtagcg cgtgccaagg gccaggtcgg acccgctcat
     4201 caagggtgcc aggaggcgtg gcaggtcgcg cggatcgtgg ctgaagtcgg catccatctg
     4261 caccaccgcc ttcgtctcac cgcgcgccaa tgcccagcgg aagccatccc gaagggcgac
     4321 acccagcccc tccttgcccg cccgatgcag gacattcagc cgtggctcgc gagcggccat
     4381 cgtgtcggcc agggagccgg taccgtcggg cgagttgtcg tcgacgacga ggagggtggc
     4441 ctctgggaga gcctcgagga tcgcatccac gagtccctcg atgttctcgc gctcgttgta
     4501 ggtcgggatc accacggtca ctccccgacc ggccgccagc ccgacatgcg gcggctccgc
     4561 gggctggctg gcgaccggcc cgggcgtcga cgtttcgccg gacatcaggc caccgccgtc
     4621 agcgcccgca ggcgcggcag cgcggcagcg accgcctgac cacgatgcga gatcaggtcc
     4681 ttttcgccct cgggcagctc ggcggtggtg atgccgctgg gcagcatgaa gatcgggtca
     4741 tagccgaatc ctcctgcgcc acgccg
//
LOCUS       DATCQR010000035         9089 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_187700, whole genome shotgun sequence.
ACCESSION   DATCQR010000035 DATCQR010000000
VERSION     DATCQR010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 9089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9089
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187700"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..637
                     /locus_tag="VIN32_01210"
     CDS             <1..637
                     /locus_tag="VIN32_01210"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY8921224.1"
                     /translation="IGTQGMVGIEAAYGARSTHTFYPANYADGARAIRLVRSRAREWG
                     IDERRVAVLGYSAGGHLASLISTQPSLYLDPRDDLATGVLARPDLVVLGYPVISFIDG
                     YSPGAFIGSVDNFLGENNPNETLRRQFSNELHVEPGHPPVFIWTTADDALVPFTHSKL
                     FAQACSRARVPVEFTLFPRGPHGMGLALDAPNEARGWTTQLLAWLAQQWGA"
     gene            complement(639..1727)
                     /locus_tag="VIN32_01215"
     CDS             complement(639..1727)
                     /locus_tag="VIN32_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY8921225.1"
                     /translation="MTAVGKALVVDDSSVNRLLLVRRLEALGLEVMQAENGVLALELL
                     AANPGAVDVVLLDVLMPELDGYETLAAMKADQSTRHIPVLIVSGVEELDSVVRCIELG
                     ASDYLTKPINASILAARINASLAAKRLHDVEVESFERQTALNRTIERQKQELSRFLSP
                     QIAALISSPEGEQLLAGHRREVTVAFCDLRGFTHFAEQAGPEEVMTLLSEYHRLMGEA
                     ITEQEGTLEHFAGDGVMVFFNDPIPQEDHVERAVRMAVAMRDRFVELASAWRQRGYEL
                     GFGVGIAVGDATLGRIGFEGRHDYGAIGNVTILASRLSSQAQAGQILLSPRAQTMLQG
                     LIESESVGELTLKGLTRPVLASNVVGLR"
     gene            complement(1724..2101)
                     /locus_tag="VIN32_01220"
     CDS             complement(1724..2101)
                     /locus_tag="VIN32_01220"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HEY8921226.1"
                     /translation="MPEPDLPVVDERILAELMASTGDDIGFVRELIQTYLADTPLQFD
                     GMSEAVEADDAAALVRPAHTLKSSSETVGAMRLSSIARELEMAGRSGSLDATARTGLD
                     AARAEWLAAAGALDAFLKRRPAE"
     gene            complement(2088..6941)
                     /locus_tag="VIN32_01225"
     CDS             complement(2088..6941)
                     /locus_tag="VIN32_01225"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEY8921227.1"
                     /translation="MTKPSPSKELAAAQKRVAELEAREAAHESAEKVQAALYRIAETA
                     SAAQDMPTFYAKIHEIVGELMYADNFYIALYDEERQQINFPYFRDEVDPDIPDPNVWD
                     PFGVANGRGSTAYLLRTGEPQFWDARRGLELIAQGEIEDPGAVSIEWLGAPLKADGKT
                     VGVIAVQTYREDRRYARDDLELLVFVAQHVGAALVRARAIEETRQRNAELAIVNEIGS
                     ALAKQLDFETIIDLVGERIRAMFDSQDMYIALYDEGTNTLRFPYDIAAGKRQATDAYD
                     LGPGLTSKVIRTRQSLRLNTQEETVAAEAIVDAVPAESWLGVPILAGERALGVIALQS
                     TRPHAFTDADERVLGTLASSMGVALENARLFDETKRLLAETDERAAELALINSVQVGL
                     SSNLDMQSMYELIGDKIRDIFDAQAVDIAVLDREAGLIRFLYEVEKGQSLHTPPIPVI
                     GLRRHVIETKEPLLVNRDLEETAKKFGQKYTLSGEPTLSALYVPLMRGSEATGVISLQ
                     NVDREDAFSDSDVRLLTTLAASLSVALENARLFDETKRLLAETDERAAELAIITSVQE
                     GLAENLDMQAMYDLVGDKIQAIFDAQVVDIGILDPTANEIRFTYTIERGVRFPNETMP
                     LIGPRRTVLESREPLVFNEDVLNEVRKYGQEGVVSGEMPKSCVYAPLVAGGEARGVIS
                     LQNLDRENAFSESDVRLLMTLAGSLSVALENARLFDETKRLLGETDRRAAELAIVNDV
                     QRGLAAQLDPHAMYELVGERSSDVFDTHVVDIAVFDIESGTMGFPFALERGVRFPDEP
                     RPIIGFRKHVLETRKPLLIGENLPERAQEMGQPDHLYGEASKSAIFVPLLVGNKILGV
                     ISLQNLDREHAFDNQDISLLTTLAASLSVALQTARLIDETQKRVAELATINSVGQALT
                     TQLELTPLLAIVGEKLQEAFEADIAFIALLDEERGFIDFPYYVERGRQEPQEPIRLGE
                     GLTSQIIQQRETIVLRNDDEVARSIVVGTPSRSFLGVPILVGDHAIGAVSVQSTTQEN
                     RFGEADARLLTTVAANIGVAVQNARLFEEAREARQAAEQANEAKSSFLAAMSHEIRTP
                     LNAVIGMSGILMDTPLNDEQRDFAETIRTSGDALLTIINDVLDFSKIEAGRVELEARP
                     FVLREAIEAALDILAPTAAKKDLELVYATDEDLPVALVGDAGRLRQIVLNLLSNAVKF
                     TDSGEVLVTVGGTQLAATRRGGSARWEIRVDVRDTGIGIPAAAMNRLFQSFSQVDASI
                     ARRYGGTGLGLAISRRLAELMDGSLTAESSGIPGEGSTFFLVARMPMAAADAVTPLRS
                     RRVEADLSGRRVLIVDDNATNRRILVAQTTKWGMVPRETGSPTEALRWLDTGERFDIV
                     LSDLLMPEMDGVEFASRASQSADGAARPPIVILSSVGVRDRDEGMLAGWLAKPVKPSA
                     LHDTIATILLGGVAAQALPAASTQTTGAGAKPLGIKHPLRILLAEDNAVNQKLALRLL
                     SQMSYTADVAGDGQQAIDALERDEFDVVLMDVQMPELDGLEATRRIRARWPDRQLQIV
                     AMTANAMAGDREACLAAGMDDYISKPIRPAELAAALERVPMAAARIVQRRPRKATKAK
                     AKSNARA"
     gene            complement(7208..7741)
                     /locus_tag="VIN32_01230"
     CDS             complement(7208..7741)
                     /locus_tag="VIN32_01230"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020420622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEY8921228.1"
                     /translation="MLSRTTTAAKGGDVSLREIQQPIKEQYRGDPAAARITLRAAGTQ
                     LEGAMSCSVDIGRALYAAEAHPGVGGPGGAACSGDLLLGALAACAQLTCQMVAANMGL
                     DLAVSVTVEGDLDLRGTLGTPEIPVGFEAIRLRISVAGEVDAERLAKLQDRTERYCVV
                     LQTLISPPSIEATWSMS"
     gene            7775..>9089
                     /locus_tag="VIN32_01235"
     CDS             7775..>9089
                     /locus_tag="VIN32_01235"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY8921229.1"
                     /translation="MKGVAEGTVTLLFTDIEGSTRLLHELGDRYGAVLDDHRRLLREA
                     FGALGGTEVDTAGDGFFYAFPRARNALLAAIDGQRALAGHEWPLPLRVRIGMHTGEPL
                     GTETGYVGLDVHRAARIMAAAHGGQIVVSQTTRHLAGDDLPSDVLLLDLGEHQLKDLP
                     TAEHLFQVVAQELIRDFPPLKSLDSRPNNLPRQLTTFVGRQDQIGEAKRVLASTPLLT
                     LTGPGGVGKTRLAIEIAGELLDEFEDGIWFVDLGPLTDPDFVVPAMAAAVGVMHAPGQ
                     PILETLTEYLRGRRVLLVLDNCEHLLEVTARSVDALLRSCGQLRVVATSREGLAIGGE
                     AVFPVPSLSLPDPEAATSGDLPQYEAIQLFVERAIAAAPGFKITDRNASAIAQICLRL
                     DGVPLALELAAARVRALPVEQIAARLDDRFRLLTGSSRVTVPRHQT"
BASE COUNT         1428 a         3059 c         2987 g         1615 t
ORIGIN      
        1 gatcggaacg cagggaatgg tcggcatcga ggcggcctac ggtgcccgga gcacgcacac
       61 gttctatccg gcgaactacg ccgacggcgc ccgggcgatc cgactggtcc ggtcgcgggc
      121 gcgtgagtgg gggatcgacg agcgccgggt cgccgtcctc ggctactcag ccggtgggca
      181 cctggcgtca ttgatcagca cccagccttc cctctatctc gatcccaggg acgacctggc
      241 gacgggcgtc ttggcacgac ccgatctggt cgtgctcggg tatccggtga tctcattcat
      301 cgacggatac agcccgggcg cgttcatcgg ttccgtcgac aacttcctcg gcgagaacaa
      361 tccgaacgag accttgcgcc gacagttctc caacgagctg cacgtcgagc ctggccaccc
      421 gccggtcttc atctggacaa cggcggacga tgccctcgtg ccgtttacgc actcgaaact
      481 gttcgctcag gcgtgcagtc gcgcgcgggt accggtcgaa ttcacgctgt ttccccgcgg
      541 gccgcacggc atgggcctcg cccttgacgc accaaacgag gcgcgcggct ggacgacgca
      601 gctgctggcc tggcttgccc agcagtgggg agcctgagct agcgcaaccc gacgacgttg
      661 ctcgcgagga cgggacgggt gaggcccttg agggtcagct cgccaacgga ttcggattcg
      721 atcagcccct gcagcatggt ctgtgctcga ggactgagaa ggatctggcc ggcctgggcc
      781 tggctcgaga ggcgcgaggc gaggatggtc acgttgccga tggcgccgta gtcgtgccgt
      841 ccttcgaagc cgatgcgccc aagggtcgca tccccgacgg cgatccccac gccgaagccc
      901 agctcgtacc cgcgctgccg ccaggcggat gccagctcca caaagcgatc gcgcatggca
      961 acggccatcc gcaccgcacg ctcgacgtgg tcttcctggg gaatcgggtc gttaaagaac
     1021 accatcacgc cgtcgccggc gaaatgctcc agcgtcccct cttgctccgt gatcgcctcg
     1081 cccatcaggc ggtgatactc gctgaggagg gtcatgacct cttcgggacc cgcctgctcc
     1141 gcgaagtggg taaagccgcg aaggtcgcag aaggcgaccg tgacctcgcg gcggtggccg
     1201 gcaagaagct gctctccctc gggcgaagag atgagcgcgg cgatctgcgg cgacaggaag
     1261 cggctgagct cctgcttctg ccgctcgatc gtgcggttga gggccgtctg ccgctcgaac
     1321 gactccactt cgacgtcgtg caggcgcttc gcggcaagtg atgcgttgat acgcgcggcc
     1381 aggatcgacg cgttgatcgg ctttgtcagg tagtccgacg cacccagctc gatgcaccga
     1441 accacgctgt cgagctcctc gacgcccgag acgatgagga cagggatgtg tcgcgttgac
     1501 tgatctgcct tcatagcggc gagcgtctcg tagccgtcga gctcgggcat gagaacgtcc
     1561 agcaacacca cgtcgacggc gccgggattg gcggccagca gctcgagcgc caggacgccg
     1621 ttctccgcct gcatcacctc cagccccaac gcctcgagcc gacgcaccag caggaggcgg
     1681 ttgacggagc tgtcgtcgac gacgagtgcc ttcccgacgg cggtcattcg gcgggtctcc
     1741 gcttgagaaa cgcgtcgagc gccccggcag ccgccagcca ctcggcgcgc gcagcgtcca
     1801 ggccggtgcg agccgtcgca tccaggctgc cacttcgccc ggccatctcc agctcgcgcg
     1861 caatggatga caggcgcatg gctcccaccg tctcgctgga cgatttgagg gtgtgcgccg
     1921 gccggacgag ggccgcggca tcgtcggctt cgaccgcctc gctcatccca tcgaactgca
     1981 gtggcgtgtc ggccaggtag gtctggatca gctcccgaac aaagccgatg tcatctccgg
     2041 tgctcgccat caactcggcc aggatccgct catcgacgac aggaaggtca ggctcgggca
     2101 ttggactttg ccttcgcctt ggttgccttc cgaggccgac gctgaacaat ccgggcggct
     2161 gccattggca cgcgctccaa cgccgcggcc agctccgcgg ggcgaatcgg cttgctgatg
     2221 taatcgtcca tgcctgccgc caggcaggct tcccggtcgc cggccatggc gttcgcggtc
     2281 atggccacga tctggagttg gcggtccggc caacgcgcac ggatacggcg cgtcgcctcg
     2341 aggccgtcga gctcgggcat ctgcacgtcc atcagtacca cgtcgaactc gtcgcgctcg
     2401 agcgcgtcga tcgcctgctg cccgtcaccg gcgacgtctg cggtgtagct catctggctc
     2461 agtagccgca gcgccagctt ctggttgacc gcgttgtcct cggccagcag gattcgcagt
     2521 ggatgcttga tgccgagcgg tttggcgccc gcgcccgtcg tctgagtcga ggctgccggc
     2581 agagcctggg ccgccacgcc gccgagcagg atggtggcga tcgtgtcgtg gagtgccgat
     2641 ggcttgaccg gcttcgccag ccagccagcc agcatccctt cgtcgcggtc gcgcacccct
     2701 acggaggaga ggatcacgat tgggggacgg gctgcgccat cggccgattg cgatgcgcgg
     2761 gaagcgaact cgacgccatc catctcaggc atgagcagat ccgacaggac gatgtcgaac
     2821 cgctcgcccg tatccagcca gcgaagcgcc tcggtcgggg agccggtctc ccgtggaacc
     2881 atgccccact tggtcgtctg cgccaccagg atgcggcggt tggtcgcgtt gtcgtccacg
     2941 atcagcacgc gccttcccga gaggtcggcc tcaacccgtc gcgagcgaag tggggtcacg
     3001 gcgtccgctg cggccatggg cattctcgcc accaggaaga aggtgctgcc ttcgcccggg
     3061 atgccgctgc tctccgcggt cagggagcca tccatcagct ccgccaggcg gcgcgagatg
     3121 gcgagcccca gacccgtgcc gccgtaccgt cgcgcgatgg aggcatcgac ctggctgaag
     3181 gactggaaga gccggttcat ggctgctgcc gggatcccaa tcccggtgtc gcgcacgtcg
     3241 accctgatct cccagcgcgc cgaaccgccc cggcgggttg ccgcgagctg cgtgccgccg
     3301 accgtgacca gcacctcccc gctatcggtg aacttcacgg cgttcgagag gaggttcagg
     3361 acgatctgcc gcaggcgtcc cgcgtcgccg accagcgcga cgggcagatc ctcgtcggtg
     3421 gcgtagacga gctccaggtc cttcttggca gccgtcggcg ccaggatgtc cagggcggcc
     3481 tcgatcgcct cgcggaggac gaagggacgg gcttccagct cgacgcgacc ggcctcgatc
     3541 ttggagaagt cgaggacatc gttgatgatc gtcagaaggg cgtcacccga agtgcgaatc
     3601 gtctccgcga agtcgcgctg ctcgtcgttg agcggggtgt ccatcaggat gccgctcatg
     3661 ccgatcaccg cgttcagcgg cgtacggatc tcgtggctca tcgcggccag gaatgagctc
     3721 ttcgcctcgt tcgcctgctc ggccgcctgg cgcgcctccc gcgcttcctc gaacaggcgt
     3781 gcgttctgca ccgccacgcc gatgttggcg gcgacggtgg tgagcaaccg cgcatcggct
     3841 tcgccgaagc gattctcctg cgtcgtgctc tgcacgctga cggcacctat cgcatggtcg
     3901 ccgaccagaa tcggcacgcc caggaatgac cgcgaggggg tcccaacgac gatcgatcgc
     3961 gcgacctcat cgtcgttccg gaggacgatg gtctcgcgct gctggatgat ctgtgacgtg
     4021 aggccctcgc caagccggat cggttcctgg ggctcctgcc tgccgcgctc gacgtagtac
     4081 gggaaatcga tgaatccccg ttcttcgtcg agcaatgcga tgaaggcgat gtctgcctcg
     4141 aacgcctctt gcaacttttc acccacgatg gcgaggaggg gtgtcagctc cagctgggtg
     4201 gtaagtgcct gaccaacgct gttgatggtc gccagctcgg ccacgcgctt ctgggtctca
     4261 tcgatcagtc gggcggtttg aagtgcgacg ctcaggctcg cggcgagcgt ggtaagaagg
     4321 gatatgtcct ggttgtcgaa ggcgtgctcg cggtcgaggt tctggagcga gatgacaccc
     4381 aggatcttgt tgccgaccag gagcggcacg aagatcgccg acttggacgc ctctccgtag
     4441 aggtgatcgg gctggcccat ttcctgcgcg cgctcgggga ggttctcgcc gatgaggagt
     4501 ggcttccgcg tctcgaggac gtgcttcctg aagccgatga ttggacgcgg ctcgtccgga
     4561 aaccgcaccc cgcgctcgag tgcgaaggga aagcccatcg tgccggattc gatgtcgaag
     4621 accgcgatgt ccacgacgtg ggtgtcaaag acgtcgctgg agcgctctcc cacgagctcg
     4681 tacatggcgt gcggatccag ctgcgcggcg aggccgcgct gcacatcgtt gacgatcgca
     4741 agctcggccg cgcggcggtc ggtctcgcca agaagacgtt tggtctcgtc gaacagccga
     4801 gcgttctcga gtgccacgct aaggctgccg gccaacgtca tcaggagccg gacatcggat
     4861 tcggagaagg cgttctcccg gtctaggttc tggagagaga tcaccccgcg agcttcgccc
     4921 ccggcgacga gcggggcgta cacgcaggac ttcggcatct cgccggaaac gacaccctcc
     4981 tggccgtatt tccttacctc gttgagcacg tcctcgttga agaccagtgg ctctcgcgat
     5041 tcgagcaccg ttcgccgtgg tccgatcaac ggcattgtct cgttcgggaa ccgcaccccg
     5101 cgctcgatgg tgtaggtgaa ccggatctcg ttcgccgtcg ggtcgaggat cccgatgtca
     5161 accacctgcg cgtcgaagat cgcctggatc ttgtcgccga ccaggtcata catcgcctgc
     5221 atgtcgaggt tctcggccaa tccttcctgg acgctggtga tgatcgccag ctcggccgcg
     5281 cgctcatcgg tttcagcgag gagtcgcttg gtctcatcga acagtcgggc gttctcgagc
     5341 gccacgctca ggctcgccgc gagcgtcgtg agcagccgca cgtccgagtc ggagaaggcg
     5401 tcctcccggt cgacgttctg aagggatatg acacccgtcg cctcgctccc gcgcatgagc
     5461 gggacgtaga gcgcggagag ggtcggctct ccggagagcg tgtacttctg cccaaacttc
     5521 ttggctgtct cctcgaggtc gcggttgacg agcagtggct ccttggtctc gatcacgtgc
     5581 cgccgcaggc cgatgaccgg gatcgggggc gtgtgcagcg attggccctt ctcgacctcg
     5641 tacaggaagc ggatcagtcc tgcctcccgg tcgaggacgg cgatatccac ggcctgggcg
     5701 tcgaagatgt cccggatctt gtcgccgatc agctcgtaca tcgactgcat gtcgaggttg
     5761 ctcgaaaggc cgacctggac agagttgatg agggccagct ctgcggcgcg ttcgtcggtc
     5821 tcggccagga ggcgcttggt ctcgtcgaag agccgcgcgt tctcgagcgc cacgcccatg
     5881 ctcgaggcga gggtgcccag cacccgctcg tctgcatcgg tgaaggcatg cgggcgtgtg
     5941 ctctggagtg cgatgacgcc aagagcacgt tcgccggcaa ggatcgggac cccaagccat
     6001 gactcggctg gaacggcatc cacgatcgcc tcagctgcga ccgtttcctc ctgtgtgttc
     6061 agccggagtg actgacgggt gcggatgacc ttcgaggtga ggccaggacc caggtcgtac
     6121 gcgtccgtcg cctgccgctt cccagctgcg atgtcgtacg ggaagcgaag ggtgttcgtc
     6181 ccttcgtcat agagcgcgat gtacatgtcc tgcgagtcga acatggcacg gattcgctcg
     6241 ccgacgaggt cgatgatcgt ttcgaaatcg agctgcttcg ccagcgcaga cccgatctca
     6301 ttgacgatcg cgagctccgc gttgcgttgc cgggtctctt cgatggcccg cgcgcggacg
     6361 agcgccgcgc ccacgtgctg cgcaacgaac acgagcagct ccaggtcgtc gcgcgcatac
     6421 cgccggtcct cccggtacgt ctggacggcg atgactccga cggtcttgcc gtcggccttc
     6481 aacggcgcgc cgagccattc aattgaaacg gcccccgggt cctcgatctc gccttgtgct
     6541 atcagttcga ggccacgcct tgcatcccag aactggggct cgccagtgcg caggagatag
     6601 gccgtagagc cacggccgtt agccactccg aaaggatccc agacgttcgg gtcggggatg
     6661 tccgggtcaa cttcgtcgcg gaagtaaggg aaattgatct gctggcgctc ttcgtcgtac
     6721 agcgcgatgt agaaattgtc ggcgtacatc agctcgccga cgatctcgtg gatcttcgcg
     6781 tagaaggttg gcatgtcctg cgcggcgctc gccgtctcgg cgatgcgata cagcgcggcc
     6841 tgcacctttt cggcagactc gtgggcggcc tcacgtgcct cgagctcagc gacccgtttc
     6901 tgcgcggcag caagctcttt cgatggtgag ggcttcgtca cacgctggct cgcacggggg
     6961 tgctgttcat gggcactcag gaacgcctca ggaatgattc ggtgccgcgg gggacggcgt
     7021 gcggcacatt ctgcccgcat cggccgctcc cgtcgatgcc gtcatgtcac cgcgatccgg
     7081 tatgcggtac caactcccaa gccgcagccc aggcgcgtcg tgtcagcttg acgatgcgcg
     7141 cctgccgcgc gatcgcggtt gctgcgtcac gagccgacgt ccactccgtg acgaagccgt
     7201 ggagcggtca gctcatcgac catgtcgcct cgatggacgg gggtgagatg agtgtctgga
     7261 gcacgacgca atagcgttcg gtccgatcct gcagctttgc gagacgctcg gcgtccacct
     7321 cccccgccac ggagatgcga aggcggatgg cctcgaagcc gacggggatt tctggcgtgc
     7381 ccagcgtgcc gcgcaggtcc aggtcaccct cgaccgtgac cgatacggcg aggtccaggc
     7441 ccatgttcgc agcgaccatc tggcaggtaa gctgggcaca ggccgccaga gcgcccagca
     7501 gcaggtcgcc cgaacaggcg gcaccgccgg gtccgccgac tcccgggtgc gcttcggcgg
     7561 catacaatgc ccgcccgatg tcgaccgagc agctcattgc gccttcgagc tgggtgccgg
     7621 ctgcccgcag cgtgatccgg gctgccgccg gatcgccgcg gtactgctcc ttgatcggct
     7681 gctggatttc cctcaggctc acgtcgcctc ctttggccgc ggttgtggtt cggctcagca
     7741 tagggtgggc gcagccggtt gggacgattg ccgcatgaag ggcgtcgccg agggaaccgt
     7801 caccctcctc ttcaccgaca tcgaaggctc gactcggctg ttgcacgagc tgggggaccg
     7861 ctacggggcc gtcctggacg accaccgccg cctgctccgg gaagcgttcg gggccctggg
     7921 cgggaccgag gtcgacaccg ccggcgacgg cttcttctac gccttcccgc gggctcgtaa
     7981 cgccctgctc gccgcgatcg acggtcagcg ggcgctggcc ggccatgagt ggccgcttcc
     8041 gctgcgggtt cggatcggca tgcataccgg agagccgctc ggcaccgaga ccggctacgt
     8101 cggtctcgat gtccatcgcg cagcgcggat catggctgcg gcccacggcg gccagatcgt
     8161 cgtctcccag accacccgcc acctcgcggg cgacgacctg ccgagcgacg tcctgctcct
     8221 cgacctcggc gaacaccagc tcaaggacct cccgaccgcc gaacacctct tccaggtcgt
     8281 cgcacaagag ctcatccgtg acttcccgcc gctgaaatca ctggattccc gcccgaacaa
     8341 tcttccccgc caactgacga ccttcgtcgg ccggcaggat caaatcgggg aggcgaagcg
     8401 cgtcctggcc tcgacgccac tgctcaccct caccggcccc ggtggtgtag gcaagacccg
     8461 ccttgccatc gagatcgccg gcgagctgct cgacgagttc gaggatggga tctggttcgt
     8521 tgacctgggt ccgttgaccg acccagactt tgtcgtcccg gcgatggccg ccgcggtagg
     8581 cgtgatgcat gctcccggcc agcccatcct ggagacgctg accgagtacc ttcgcgggcg
     8641 gcgggttctc ctcgttctcg acaactgcga gcacctcctg gaagtgactg cgcgctccgt
     8701 cgacgcgttg ctgcgcagct gcgggcagct gcgcgtagtc gccaccagcc gagaggggct
     8761 cgccatcggt ggggaggccg tcttcccggt cccgtcgttg tccctccccg atcccgaggc
     8821 cgcgacctcg ggcgacctgc cgcagtacga ggcgatccaa ctgttcgtcg agcgcgcaat
     8881 cgcggctgcc cccggattca agatcaccga tcgcaacgcc tcggcgatcg cccagatctg
     8941 tctccgcctc gatggcgtcc cgctcgccct cgagctggcc gcggcccggg tccgcgcgct
     9001 cccagtcgag cagatcgcgg cccggctcga cgaccggttc cgcctgctga ccggctcgag
     9061 ccgggtcacc gttccacgtc accagacgc
//
LOCUS       DATCQR010000036        10101 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_64048, whole genome shotgun sequence.
ACCESSION   DATCQR010000036 DATCQR010000000
VERSION     DATCQR010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 10101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10101
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64048"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1394)
                     /locus_tag="VIN32_01240"
     CDS             complement(<1..1394)
                     /locus_tag="VIN32_01240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921230.1"
                     /translation="MTRDSVRERGARPIERGLPLSPANLVLLLIVVGVLLRLLIAGPY
                     LPFSGLRNDVGDFAIWANRMAAGGPGQFYQPGYLADYPPGYMYVLWGLGAIAQVLRPI
                     TGVLITPGLVKLPAILADGAVAWLLFVYARRWGSGWLGSWSGERLGIVAAVIYLFNPG
                     TIFDSAVWGQVDAVGTLVILASLYLLARGRTEAAAAGAVIAMLVKFQFGFLIPVVAVV
                     GIKRHLFGRSSDPDHDGRRDPIRVLTSLASGLGTLVILILPFRLAIWAPADPTFSLVD
                     RLIAASNTYQGLSINAFNLWRNPLTGLGDAYRWGCDSPPPTCSGGEGIAFTLGSMLVS
                     WQLVGAVLFGIVALITLWMVARRDDPAGLMVGALTLAVAFFALPTRVHERYLFPALAL
                     AAPLVARTWKWAVLYGVLTLSFFANVYWLYTTDFSWVGGEFPMNPGLGRLAMPRDPFL
                     ASVVFNDTGVYLLSL"
     gene            1511..2263
                     /locus_tag="VIN32_01245"
     CDS             1511..2263
                     /locus_tag="VIN32_01245"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY8921231.1"
                     /translation="MTDAAPSARPRLTFFFPAYNEQENIARTVELALSQIGPLVPSIE
                     VLAIDDGSTDQTATLADALAAADPRVRVHHQPNRGYGGAIKAGFEQARGELICFSDGD
                     LQFDLREMSRLLDRLADERKPVDAVIGFRIRRRDPFHRIFIAKTYNAIVSVAFGLRVR
                     DIDCAMKLFRREVFDGLRLDAEGPFLSAELLIKLRARGVRIAQVGVNHFPRAAGTNTG
                     ASLGKILRTFRDLAKLRWDLWTKREEVLGRRK"
     gene            complement(2503..3033)
                     /locus_tag="VIN32_01250"
     CDS             complement(2503..3033)
                     /locus_tag="VIN32_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HEY8921232.1"
                     /translation="MRLIEMVVESVRVHMLSSQHVVILKESDRERYLPIWIGPSEANA
                     IAMKLQGLNAERPLTHDLLVSIVAALNSSISRVVVTHVTDGTFHARLYLETADGGEAE
                     IDSRTSDAIAVAVRAGSTIYVDERVLDEAGVEPDRAEPGDVPDEKLAIFREFVNSLDV
                     DLESGEKGPPSEELGS"
     gene            complement(3053..5275)
                     /locus_tag="VIN32_01255"
     CDS             complement(3053..5275)
                     /locus_tag="VIN32_01255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921233.1"
                     /translation="MSNRLATRIGLLAGEDRAPDALDLVRFREPTSGSEVRTKGSLFL
                     LAQVTGGDAALGRAAAEALETIERDYYYDLSAGATGAISKALTAANRLLYHQRSRLGL
                     TRRGGVSVVALVVRGREGHVAKLGPAAAVIVRGERMFELPPPPSVDEEDPRVRERRVA
                     DSLGEALEIRPYTWEGELAAGDRVALLSRNVAQVVGVEEVQRALATLRPAAAVEHLHQ
                     LFQIREGHGSDGMLAIELIELAATAATHQLEPVHPHEELAGLPDRSPVPLADAIGRTL
                     HRGGDAIDAVQRALGHGLLVGVNMALAFVPRRRAQYPTSIPRTAVREEGRRRRRGLVG
                     IGAVAALLAIGASVASLPNPRPTDAILRASIARAAIAEALDLLGTVEKRVDGRDLIDR
                     DPTRAASLLTDSLAAVERASEAGVASASLEPLRGRLEGGLDSIFAVGRLLQSSTVVDL
                     AAAFTATDPIDMVMASDGSLWVAEVGRGRVIRVDPASGKATVPYRAGQELGGVTAGAP
                     WMIATAATDVVVLDRDRHAWRFDLVEQVAHPMGLPGLAAVSTESHLLTALQHRPPLEI
                     FNLYLADAASGDVLKWTPGDRIPIRYPDPPEPYLLEPPDLPVAQARDMFADANLWLLQ
                     PHTVTRVNFGTPLAQADYSFDPPPDGSLRPDLDYRLLDGATIGERETFYVYDAANARI
                     LAYQRADGSFLRQWVAPRSGPQAGLLDEVRALSVASVADGPPVAYLLTPTRIVRVVLE
                     "
     gene            complement(5318..5668)
                     /locus_tag="VIN32_01260"
     CDS             complement(5318..5668)
                     /locus_tag="VIN32_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921234.1"
                     /translation="MSRTEEAASAVQRALLACMGPGAAEQIALAQARLAWQEAVAAAG
                     LEREGLSSRLVTVRAGTAQVEASEPILAQELGLRAEALMAAANHRMDGRPGATIVLTR
                     VAVSVARSGGGRSL"
     gene            complement(5665..6840)
                     /locus_tag="VIN32_01265"
     CDS             complement(5665..6840)
                     /locus_tag="VIN32_01265"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEY8921235.1"
                     /translation="MRLTRLLLEDFRSYASAELHPEPGLTIVAGPNGAGKTNLLEAVF
                     VAVTGRSHRAGTDAELVRHSRPFARVRLDLSDPQGATARVELLVPAEDAPPEIRKRVS
                     VNGLPRRASAIGDTVRVVLFRPEEMLLLVGAPSERRRFMDAILSQRHRAAARDLAELG
                     RILAQRNALLRAIRREEATPDNLAFWDEQLAAVGARVLAARLDLVAELAARIPALHDA
                     VAPADEQGEQVRLTYADTLKDAWPERPAASGAAPPLEQLEAAYRRRISDLREKELWNG
                     VSLVGPHRDDLRVELAGREVATHASRGQQRTIILALKLAETDLLGSDDAPTPIVLLDD
                     VFSELDADRSDRSLSLLLERGQVLVTMADLATLPAGRRGVTIWQVGEGRLSQAPRVA"
     gene            complement(6875..8023)
                     /gene="dnaN"
                     /locus_tag="VIN32_01270"
     CDS             complement(6875..8023)
                     /gene="dnaN"
                     /locus_tag="VIN32_01270"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEY8921236.1"
                     /translation="MVVKDEVGMNVSVMQENLARGLGVVGRAVSSRATLPVLANVLLK
                     TENAGVKLTATNLEIGINCWVPGKVSDEGEITVPAKLLTELVSSLPNQRIDLQFSAKD
                     RTLKVTCGGSRSSIKGIEADEFPVVAAIGDTPITSADSRTLREALGEVVFAAASDESR
                     PILTGVLTRFAGDRMTLAAADNYRIAVRGLALDKPVTPEITIVVPARSYQELIRIMPD
                     AEEPIEITVTPNKSQILFHVNGIDLVSRLIEGQFPNYEPVIPTGHTSRAVLDREAFLA
                     GARRASIFARDSANIVKIEIGGEAAEGVAITAHAADIGDAADALEATIDGQPTSIAFN
                     ARYLIDVLSNLGADEAALELSGPLAPGVIRGVGKDDYVHVIMPVRTAS"
     gene            complement(8325..9665)
                     /gene="dnaA"
                     /locus_tag="VIN32_01275"
     CDS             complement(8325..9665)
                     /gene="dnaA"
                     /locus_tag="VIN32_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006562115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEY8921237.1"
                     /translation="MDAKQVWRAVLGELQVSLSPANFETWLKDTSLVELDENRYRIAA
                     PNGFARDWLDNRYRTLISQTLARVVGGSVQVEFVVADAPGPVPGQSDDEALPLPPASA
                     GGNTVNLNGRYAFSNFIVGSANRLAHAAALSVAERPGHAYNPLFLYGGVGLGKTHLMH
                     AIGNAVSIKFPRKRILYATSEKFTNDFINSIREQKMEDFRNRYRRIDVLLIDDIQFIA
                     ERERTQEEFFHTFNTIHEAGKQIVLSSDRPPKAITTLEERLRSRFEWGLIADLTPPDL
                     ETRIAILRSKAEDQLNLIPGEVIEFIARKVVSNVRELEGALNRVVAYASMSGMPINNE
                     LASAVLSNVMYNPKKRSITPQRIVRAVADYYGVNLDQLRSSKRDKAIVVPRQIAMYLI
                     REETDISLLRIGAELGGRDHSTVLHAYDKIARELAENDEMRREVSAVREMIYSE"
     gene            9995..>10101
                     /gene="rpmH"
                     /locus_tag="VIN32_01280"
     CDS             9995..>10101
                     /gene="rpmH"
                     /locus_tag="VIN32_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018700622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEY8921238.1"
                     /translation="MKRTYQPKKRARKREHGFRARMASKGGRRVLARRRA"
BASE COUNT         1545 a         3456 c         3515 g         1585 t
ORIGIN      
        1 agcgagagga ggtagacccc cgtgtcgttg aagaccaccg atgcgaggaa tgggtcgcgc
       61 ggcatcgcca ggcgtccgag gccggggttc atcgggaact cgccgccgac ccatgagaag
      121 tcggtggtgt agagccagta gacgttggcg aagaacgaca gggtcagcac cccgtagagc
      181 accgcccact tccaggtgcg agccaccagg ggagccgcca gggccagggc ggggaagagg
      241 tatcgctcgt ggacccgggt cggcaaggcg aagaacgcaa cggccagggt cagtgcgcca
      301 accatgaggc ccgccgggtc gtcgcgacgg gcgaccatcc acagcgtgat cagggcgacg
      361 atcccgaaca ggaccgcccc caccagctgc cagctgacca gcatggagcc gagggtgaat
      421 gcaatcccct ccccgccaga gcaggtgggc ggcggtgagt cgcatcccca gcggtaggcg
      481 tcgcccagcc cggtcaacgg gttgcgccac aggttgaagg cattgatcga cagcccctgg
      541 tacgtgttgc tcgccgcaat gaggcggtct acgaggctga aggtcggatc ggcgggtgcc
      601 cagatggcga gcctgaacgg gaggatgagg atcaccaggg tgcccagccc gctggcgagg
      661 gaggtgagga cccggatcgg gtcgcgccgg ccgtcgtgat cgggatccga tgagcggccg
      721 aagaggtggc gcttgatccc caccaccgcg accaccggga tcaggaagcc gaactggaac
      781 ttcaccagca tggcgatcac cgcgccggcg gctgcggcct cggtccggcc tcgggcgagg
      841 aggtagaggc tcgccaggat gaccagcgtc ccgaccgcgt cgacctggcc ccacaccgcc
      901 gagtcgaaga tggtgcccgg gttgaagagg tagatgacgg ccgccacgat gcccaggcgc
      961 tcgccgctcc aagagccaag ccagccgctc ccccagcgcc gggcgtagac gaagaggagc
     1021 caggcgaccg ctccatcggc gaggatcgcc ggaagcttga cgagcccggg ggtgatcagg
     1081 accccggtga tcggccggag cacctgggcg atcgctccca ggccccacag cacgtacatg
     1141 tagcccggcg ggtagtccgc caggtagccc ggctggtaga actggccggg acccccggcc
     1201 gccatccggt tggcccagat cgcgaagtcg ccaacgtcgt tgcgaaggcc gctgaatgga
     1261 aggtacggcc ctgcgatgag gaggcgcagc aggacgccaa cgacgatgag caggaggacc
     1321 aggttcgcgg gactgagcgg caggccgcgt tcgattggcc tcgcgccccg ctccctgacg
     1381 gagtcgcggg tcatcggtgg cggcacctcg ctatcggcgc ctgggtctgt cgttcgccaa
     1441 cgtggggcgg agtatacatc cgggtttggg acgccccgcg atggcggccg gttgcgttag
     1501 ggtaccgccc gtgaccgatg ccgcgccgtc tgcccgtccg cgcctgacct tcttcttccc
     1561 cgcctacaac gagcaggaga acatcgcgcg gaccgtcgag ctggcactct cccagatcgg
     1621 cccattggtc ccttcgatcg aagtcctggc gatcgacgat ggctccaccg accagacggc
     1681 cacactcgcc gatgcgcttg ccgcggccga tccgcgggtg cgggtccacc accagccgaa
     1741 ccgtggctac ggcggcgcga tcaaggcggg cttcgagcag gctcgaggcg agctcatctg
     1801 cttctcggac ggcgacctcc agttcgacct gcgcgagatg agccggctgc tggatcggtt
     1861 ggcggacgag cgtaagccgg tcgacgcggt gatcggcttt cgcatcaggc gtcgcgatcc
     1921 attccaccgc atcttcatcg ccaagaccta caacgcgatc gtgtccgtgg ccttcgggct
     1981 tcgcgtgcga gatatcgact gcgccatgaa gctcttccgg cgcgaggtct tcgacgggct
     2041 gcgcctcgat gcagaggggc cgttcctgtc ggcagagctg ctgatcaagc tgcgggcgcg
     2101 tggggtgcgc atcgcccagg tcggcgtcaa tcactttccg cgcgctgccg gcaccaatac
     2161 cggcgccagc ctcggcaaga tcctgcgcac cttccgcgac cttgcgaagc tgcgctggga
     2221 cctgtggacg aagcgcgagg aggttctcgg ccggcggaaa tgaccggggg ccatcgttcc
     2281 tgccagattc acaccaggtg accgcgaagc gggcctcgac tgtggtcttg agccagattc
     2341 acactgggtg accgcgcaat cggctagagg ttggatgcgg gagccagatt cacgctccgc
     2401 gtgaatctgg cagcttcagg caggggcgcg agcgtctcat gtgcccctgg tgtgaatctg
     2461 gcacgtgcgc cagcgatccc agttccccga agccgctgcg cttcacgatc ccagctcctc
     2521 gctgggcgga cccttctcgc cgctctccag gtcgacgtcg agcgagttga cgaactcgcg
     2581 gaagatggcg agcttctcat ccggcacgtc gcctggttcg gcgcggtcag gctccacgcc
     2641 cgcctcgtcg aggacccgct cgtcgacgta gatggtcgat cccgcgcgca ccgccaccgc
     2701 gatcgcgtcg ctggtgcgcg agtcgatctc ggcctcgccg ccgtccgcgg tctccaggta
     2761 gagccgggcg tggaaggtgc catcggtgac atgcgtgacg acgacgcggc tgatgctcga
     2821 gttcagggcg gcgacgatgc tgaccagcag gtcgtgagtc agcggacgct ccgcgttgag
     2881 gccctgcagc ttcatggcga tggcgttggc ctctgacgga ccgatccaga tcggcagata
     2941 ccgctcccga tcggactcct tcaggattac cacgtgctgg ctcgagagca tgtgcacccg
     3001 cacgctttcg accaccatct cgatcagcct caccggccga ttctagatct tgctactcga
     3061 ggacgacgcg gacgatccgt gtcggcgtca gcaggtaggc gactggcgga ccatccgcga
     3121 ccgaggccac gctgagggcc cgaacctcgt ccagcaggcc cgcctggggg ccgctgcgcg
     3181 gcgccaccca ctgtcgcagg aacgagccat cggcacgctg ataggccagg atccgggcgt
     3241 tggccgcgtc gtagacgtag aaggtctcgc gctcgccgat ggttgcgccg tcgaggagcc
     3301 gatagtcgag atccgggcga aggctgccgt cgggtggcgg gtcgaacgag tagtccgcct
     3361 gggcaagcgg cgtcccgaag ttgacgcgcg tcacggtgtg cggctggagc agccacaggt
     3421 tggcatccgc gaacatgtcg cgcgcctggg cgaccggcag gtccggcggc tcgaggaggt
     3481 atggctcggg cggatccggg taacggatgg ggatcctgtc gccgggggtc cacttgagca
     3541 cgtcgccact cgccgcgtcg gccaggtaca ggttgaagat ctccagcgga ggccgatgct
     3601 ggagagcggt cagcaggtgg ctctcggtcg atacggcggc aagcccgggc agccccatcg
     3661 ggtgcgcgac ctgctcgacc aggtcgaagc gccacgcatg ccggtcccga tccaggacca
     3721 ccacgtcggt cgccgcggtg gcgatcatcc acggggcgcc ggccgtcacg ccgccaagct
     3781 cctggccggc ccgatacggg acggtcgcct ttccgctcgc cggatcgacc cggatgaccc
     3841 gcccgcgacc gacctccgcg acccagagcg agccgtcgct ggccatcacc atgtcgatcg
     3901 ggtcagtcgc cgtgaaggca gccgccaggt cgaccacggt gctgctctgc aggagtcgcc
     3961 cgacggcgaa gatggagtcc aggccaccct cgagccgacc acgcagcggc tcgagcgagg
     4021 ccgatgcgac gcctgcctcg gaggcccgct cgaccgcggc aagtgagtcg gtgaggagcg
     4081 aggctgcccg cgtgggatcg cggtcgatca ggtcgcgccc atccacccgc ttctcgacag
     4141 tgccgagcag gtccagcgcc tcggcgatcg ccgcccgcgc gatcgaggct cgcaggatgg
     4201 catcggtggg tcgtgggttc ggcaggctgg ccacgctcgc accgattgcc agcagcgcgg
     4261 ccacggcgcc gatcccgacc agcccgcgcc ggcggcgtcg tccctcttcg cggaccgcag
     4321 tccgagggat gctggtcggg tactgggcac gtcggcgcgg cacgaaggcg agtgccatgt
     4381 tgacccccac cagcaggcca tgccccagcg cgcgctggac cgcgtcgatc gcgtcgccgc
     4441 cccgatgcag ggtgcggccg atcgcatccg ccaggggaac cgggctgcgg tccgggaggc
     4501 cggccagctc ctcgtggggg tggaccggct cgagctggtg ggtggcggcc gtcgcggcca
     4561 gctcgatcag ctcgatcgcc agcatcccgt ccgagccatg gccctcccgg atctggaaca
     4621 gctggtgcag gtgctcgacc gctgccgccg ggcgcagggt ggccagggcc ctctggacct
     4681 cctcgacgcc gaccacctgc gccacgttgc gcgacaggag cgcgacccgg tcgccagcgg
     4741 ccagctcccc ctcccaggta tacggcctga tctcgagcgc ctcacccagc gagtcggcga
     4801 cgcggcgctc gcggacccgc ggatcctcct catccacgga tgggggcggc ggcagctcga
     4861 acattcgctc gccccgcacg atgactgcgg cggcaggccc gagcttggcc acatgcccct
     4921 cgcgcccgcg aaccaccagg gcgacgacgc tcacccctcc gcggcgcgtc aaccccagcc
     4981 gggatcgctg gtggtagaga aggcggttgg cggcggtgag cgccttgctg atcgctcccg
     5041 tggcgccggc ggagaggtcg tagtagtagt cccgctcgat cgtctccagc gcctcggccg
     5101 cagctcgacc cagcgcggcg tcgccgccgg tgacctgcgc cagcaggaag aggctgccct
     5161 tggtgcggac ctccgacccg ctcgtcggct cgcggaagcg gaccaggtcg agcgcgtcgg
     5221 gggcgcggtc ctcgccggcc aacaggccga ttcgggtggc caggcgattg ctcatcggga
     5281 atccgcggac tcgagcgaag gacggcgacg cgcccgatta tagggagcgg ccacccccgg
     5341 aacgagccac cgaaacggcg acccgggtca ggacgatcgt cgcccccggc cgcccatcca
     5401 tgcggtggtt ggcggccgcc atgagcgcct cggcgcgcag gccgagctcc tgcgccagga
     5461 tcggttcgga ggcctcgacc tgggcggtac cggcgcggac ggtgacgagc cgggacgaga
     5521 gcccctcgcg ctcgagtccg gccgcggcga ccgcctcctg ccatgcgagt cgcgcctggg
     5581 ccagcgcgat ctgctccgcg gccccgggac ccatgcatgc aagcagcgcc cgctgcacgg
     5641 cgctggcggc ctcctcggtg cggctcatgc cacccgcggc gcctgggaga ggcgcccctc
     5701 gccgacctgc cagatcgtga cgccccttcg tccggcgggg agcgtcgcca ggtcggccat
     5761 cgttacgagc acctgaccac gctccaggag aagcgacagc gaccggtcgg atcgatccgc
     5821 gtcgagctcg gagaagacgt cgtccagcag gacgatgggg gtcggggcgt catcggatcc
     5881 gagcaggtcg gtctcggcca acttcagggc caggatgatg gtccgctgct ggccgcggct
     5941 ggcgtgcgtg gccacctctc gacccgcgag ctcgacccgc aggtcgtcgc ggtgcggccc
     6001 caccagcgag accccgttcc acagctcctt ctcccgcagg tcgctgatcc gccgccggta
     6061 tgccgcctcc agctgctcga gcggtggcgc cgccccactg gccgcgggcc gctcgggcca
     6121 cgcatccttg agcgtgtccg cgtaggtcag acgcacctgc tctccctgtt cgtcggcggg
     6181 ggcgaccgcg tcgtgcagtg ctgggatccg tgccgcaagc tcggccacca ggtccagccg
     6241 cgccgcaagc acccgcgccc ccaccgcggc gagctgctca tcccagaacg cgaggttgtc
     6301 gggcgtcgcc tcctcgcgcc ggattgcgcg cagcagtgcg ttgcgctggg cgaggatccg
     6361 ccccagctcg gccaggtcgc gggccgcggc gcggtggcgc tgcgagagga tcgcatccat
     6421 gaatcggcga cgctcggacg gcgcgccgac caggagaagc atctcctccg gacggaacag
     6481 gacgacgcga accgtgtcgc cgatcgccga ggcgcggcgc ggcaggccgt tgacgctgac
     6541 ccgcttgcgg atttcggggg gcgcgtcctc ggccggcacc agcagctcca ctcgtgccgt
     6601 ggcgccctgc gggtcggaca ggtcgagccg gacgcgggca aagggccggg agtggcgtac
     6661 cagctctgcg tccgtccccg cccgatgcga gcgccccgtg acggcgacaa agaccgcctc
     6721 cagcaggttg gtcttgccgg caccgttcgg gccggccacg atggtcaggc ccggttcggg
     6781 gtggagctcc gcgctggcgt agctgcggaa gtcctcgaga aggagtcgag tcaggcgcac
     6841 cgctctgctc cggccaccgg cgtggccccg ctccctagga tgcggtccgg accggcatga
     6901 tcacgtggac gtagtcgtcc ttgccgaccc cgcggatgac gcctggcgcc agggggccag
     6961 agagctccag ggcggcctca tcggccccaa ggttgctcag gacgtcgatc aggtagcgcg
     7021 cgttgaatgc gatgctcgtc ggctggccat cgatggtggc ctcgagcgcg tcggctgcat
     7081 ctccgatgtc ggctgcatgg gccgtgatcg cgacaccctc cgcagcctcg ccgccgatct
     7141 cgatcttgac gatattggcg ctgtcgcgcg cgaagatgct tgcccgccgg gcgcctgcca
     7201 ggaacgcctc ccgatccagc accgcacggc tggtgtgccc ggtcgggatc accggctcgt
     7261 agttggggaa ttgcccctcg atcagccggc tcaccaggtc gatcccgttc acgtgaaaca
     7321 ggatctggct cttgttcggc gtgactgtga tctcgatcgg ctcttcggcg tccggcatga
     7381 tccggatcag ctcctggtag gagcgggcgg gcacgacgat cgtgatctcc ggggtgaccg
     7441 gcttgtcgag cgccaggccg cggaccgcga tgcggtagtt gtcagccgcg gcgagcgtca
     7501 tccgatcccc cgcaaagcgg gtcagcacgc cggtcaggat cgggcggctc tcgtcgctgg
     7561 ccgccgcgaa gacgacctcg ccaagggcct cgcgcagtgt gcgcgagtcc gcgctggtga
     7621 tcggcgtgtc gccgatcgcg gccacgaccg ggaactcgtc ggcctcgatc cccttgatcg
     7681 aggagcggct ccctccgcac gtcaccttga gcgtgcggtc cttcgctgag aactggaggt
     7741 cgatgcgctg gttcgggagc gacgagacga gctcggtgag cagcttcgcc ggcaccgtga
     7801 tctccccttc atcgctcacc ttgcccggaa cccagcagtt gatcccgatc tcgaggttgg
     7861 tggcagtcag cttgacgccc gcgttctcgg tcttgagcag gacgttggcc agcaccggga
     7921 gggtggctct gctgctgacg gcacgcccca ccacccccag gccgcgcgcc aggttttcct
     7981 gcatgaccga tacgttcatc ccgacctcat ccttgacgac catttgggtt ctccaccggc
     8041 ctgttcggca ctctatgaat cgaagagaag gatactcatc cgtagccgtc tccagtaggc
     8101 gtgtggacgg tgtggatcgt cacgctcgtc cgtgctcggc accggtgccg ataactgcct
     8161 tccgtcggtg ggcatctttc ctcgtgatga gcctctgctg tggatggctc gccccacccg
     8221 gttgacaacc ctcccgtctg ccttccgcga cccggcggac ccgtccacaa tgcgcgacgt
     8281 ttctccaccg tccaggccgt cttgtccacg acctgaggtc cgggtcactc ggagtagatc
     8341 atctcccgca ccgcgctcac ctcgcgacgc atctcgtcat tctcggccag ctcccgcgcg
     8401 atcttgtcgt aggcgtgcag cacggtcgaa tggtcgcgcc ccccgagctc ggcgccgatg
     8461 cgcaggagcg agatgtcagt ctcctcgcgg atcaggtaca tcgcgatctg gcgcggcacg
     8521 acgattgcct tgtcgcgctt cgacgagcgc agctggtcca ggttgacccc gtagtaatcg
     8581 gcgacggcgc gcacgatgcg ctgcggcgtg atggagcgct tcttggggtt gtacatgacg
     8641 ttcgacagga ctgcgctggc cagctcgttg ttgatcggca tgcccgacat ggacgcgtag
     8701 gccaccaccc ggttgagcgc tccctccagc tcgcggacgt tgctcaccac cttgcgcgcg
     8761 atgaactcga tcacctcgcc cgggatcagg ttgagctggt cctccgcctt gctgcgcagg
     8821 atcgcgatcc gggtctcgag gtccggcggg gtcaggtctg cgatgagccc ccattcgaag
     8881 cgggagcgga gccgctcctc cagggtggtg atcgccttcg gcgggcggtc gctggacagg
     8941 acgatctgct tccccgcctc gtggatcgtg ttgaaggtat ggaagaactc ctcctgggtc
     9001 cgctcgcgct cggcgatgaa ctggatgtcg tcgatcagca gcacgtcgat gcggcgatat
     9061 cggttccgga agtcctccat cttctgctcg cggatggaat tgatgaagtc gttggtgaat
     9121 ttctcgctgg tcgcgtacag gatccgcttg cgcgggaact tgatcgatac cgcgttgccg
     9181 atggcgtgca tgaggtgggt cttgcccagc cccaccccgc cgtacaggaa gagggggttg
     9241 taggcatgtc cgggccgctc ggcgacggag agggcggcgg cgtgcgccag gcggttggca
     9301 ctgcccacga tgaagttgct gaaggcgtag cgcccgttca ggttgacggt gttgccgcct
     9361 gcagaggcgg ggggcagcgg gagcgcctcg tcgtcggact ggcccggcac cggacccggg
     9421 gcatcggcca ccacgaactc gacctggacc gatccgccca cgacgcgggc gagggtctgg
     9481 ctgatcagcg tgcggtatcg gttgtcgagc cagtcccggg caaagccgtt cggggctgcg
     9541 atgcgatagc ggttctcgtc gagttccacg agcgacgtgt ccttgagcca ggtctcgaag
     9601 ttggcgggcg agagagagac ctgcagctca cccaggacgg cacgccatac ctgcttcgca
     9661 tccatcgata gccaactccc aatcgatcta gccgcggcgt tccggcaccg gtcgaggacc
     9721 tccctcgggg cgcacgggac ccggtcagat gggcgtcggg cctcgggggt cgcggtagtg
     9781 taccagcacc ctttcggcac cggcaatgga ttcgttcaag ttgccgccgt ggcgggttgg
     9841 aggtttgaca ctccgctcgg cgcctcgcta gaatcgccgg gcagagcgag acgggccgca
     9901 ccacagggcc atcgcgtgag gctggcgaag acgccagcca ttcttgtgtt atcgacccgg
     9961 atcgccgccg ctgaacgagg acaggcacgc actcatgaag aggacctacc agcccaagaa
    10021 gcgcgcccgg aagcgggaac acggcttccg cgctcgcatg gcttcgaagg gtggtcgacg
    10081 cgtgcttgcc cgtcgtcggg c
//
LOCUS       DATCQR010000037         5365 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_17317, whole genome shotgun sequence.
ACCESSION   DATCQR010000037 DATCQR010000000
VERSION     DATCQR010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5365
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17317"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..763
                     /locus_tag="VIN32_01285"
     CDS             <1..763
                     /locus_tag="VIN32_01285"
                     /inference="COORDINATES: protein motif:HMM:NF020067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HEY8921239.1"
                     /translation="APGRIAAGVLSAVLIGGLLALVNGPWLRVGRVAWAGERFTPDTQ
                     LRRALASLDGSALLTLDAAAVAAKIQRLPAVSRATVEAVLPDAVQVHIVEKEATFVWQ
                     TSAVRLVGVADGTLIAQLAPGADLPPDLAALPSIDDRRSQSRSLTAGDRIDPSSLAAA
                     LRLSEVDPAALGSTAVALDVRLTDNDGFLLVSVQPSWQADFGLYPNSDFGEPAALQKR
                     VEAQVTAVRTLFSFEGEAQMGWVDARDPRRVYWRP"
     gene            818..1141
                     /locus_tag="VIN32_01290"
     CDS             818..1141
                     /locus_tag="VIN32_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014904588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small basic family protein"
                     /protein_id="HEY8921240.1"
                     /translation="MWLALGGLALGVALGLALNVNVSPDLARYSAVGILAGLDSVLGA
                     IRAELDAHYDNRIFISGFVTNALVAAALTFIGDRLGIDLYLVALFAFGLRIFQNVALI
                     RRHFL"
     gene            1193..2431
                     /gene="ftsA"
                     /locus_tag="VIN32_01295"
     CDS             1193..2431
                     /gene="ftsA"
                     /locus_tag="VIN32_01295"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEY8921241.1"
                     /translation="MDRETVLVGIDAGTTKVTTLIGEVSRGGDVNIIGYGIAPSVGMK
                     KGMVANIDQTVNSIAASLEKAERLSGYKISSAFVAVGGSHISSQNSRGVVAVSGHKRE
                     VSREDVARATEAAKAIQVPSNREILHVIPRGYIVDGQEGIKDPLGMSAVRLEVETHIV
                     AGAATSIQNLSKCIANANVEIDELVISSLAAAEATLTDTEKELGVMIADIGGGTTDLA
                     IFTDGAVYHSAVLPVGAINVTNDVAIGLRTSLDLAEEIKIKHGSANLADIAPEELLNV
                     AVLGEGGGQTIQRKKLCEIIEARMSEIFSLIGEEIKRSGHAGLLPAGVVLTGGGARLA
                     GVAELAREVLQMPVRVASPQGVGGLMDQLSNPAFSTPIGLLLWGAHHIGSEPISFTVG
                     SPMGGGVGRFMDWLRGLFPG"
     gene            2632..3891
                     /gene="ftsZ"
                     /locus_tag="VIN32_01300"
     CDS             2632..3891
                     /gene="ftsZ"
                     /locus_tag="VIN32_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006675639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEY8921242.1"
                     /translation="MPLRSDAEHFALIKVIGVGGGGSNAVNRMIRAEMMGVEFISVNT
                     DAQALLQSDAPHKIRIGDKLTRGLGAGADPMVGQRAAEEDSEKIYEALKDADMIFITA
                     GMGGGTGSGAAHVIAEIAKDLGALTVGVITKPFSFEGVRRKLVAEQFSELLKEKCDTL
                     ITIPNDRLREVVDKKTSILDAFRVVDDVLRQGVQGISDLITVPGLINLDFADVKTIMR
                     DAGSSMMGIGIGTGDNRAVEAARAAVMSPLLEVNITGARGILFNVTGGSDLGLFEVNE
                     AAEVIKEAADPEANIIFGTVIDDRMRDEVKVTVIATGFDATRKPRPAARAVTPASEVA
                     TGIEASLDERSREILAEIERDRAERQALEDQMPEASPFAVGEKPSETTARTGERQAIP
                     VRGSGLDRPTYGETDLDIPSFLRRKSE"
     gene            3962..4696
                     /locus_tag="VIN32_01305"
     CDS             3962..4696
                     /locus_tag="VIN32_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEY8921243.1"
                     /translation="MLPVDEPPLTAELIRSRHDALLRRLCAAAEAAGRNPDAVRLVAV
                     TKGFGTAVIRDATAAGLSRFGENRVQEAIPKMSAAPEAEWHLVGRLQANKARPAVRGF
                     AAIHSVDSLELLGRLDRIAAEESRAPIALLQVNVTAETSKAGFALDWFEEAVGRPGSL
                     SAAIAGLTAVRLAGLMTIGPAGAATAESRRAFATLRGLRDQLQQLTGHPLPELSMGMT
                     ADADAAVAEGATLVRIGTAIFGPRPA"
     gene            4724..4975
                     /locus_tag="VIN32_01310"
     CDS             4724..4975
                     /locus_tag="VIN32_01310"
                     /inference="COORDINATES: protein motif:HMM:NF014389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEY8921244.1"
                     /translation="MPLVIVNFLELLVSALMLLVLARVVVSWLAPTGGGGVVAFIYQA
                     TEPILAPIRRVVPPSGGLDWAPLIAMLVLGLLLRLVVRL"
     gene            5028..5105
                     /locus_tag="VIN32_01315"
     tRNA            5028..5105
                     /locus_tag="VIN32_01315"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5063..5065,aa:Val,seq:cac)
     gene            5172..>5365
                     /locus_tag="VIN32_01320"
     CDS             5172..>5365
                     /locus_tag="VIN32_01320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8921245.1"
                     /translation="MRLLEPLQERDFALLTAGSLVSLLGDGFFSVALAWQVYEISNLP
                     TALSIVGVAWTLPVVLFVLLG"
BASE COUNT          824 a         1824 c         1875 g          842 t
ORIGIN      
        1 ggcccccggt cggatcgccg ccggcgtgct gagcgcggtg ctgatcggcg ggctgctggc
       61 cctcgtcaac gggccgtggc tgcgggtcgg ccgggtcgcc tgggccggcg aacgattcac
      121 cccggacacc cagctgcggc gtgctctggc gtcgctcgat gggagcgccc tgctcacgct
      181 cgatgccgcc gcggtggccg cgaagatcca gcgcctgccg gcagtctcgc gggccacggt
      241 ggaggcggtc ctccccgacg cggtgcaggt tcacatcgtc gagaaggagg cgaccttcgt
      301 gtggcagacg agcgccgtcc gcctggtcgg cgtcgcggat ggcacgctca tcgcgcagct
      361 cgcgccaggg gccgatctgc cccccgacct cgccgcactg ccctccattg acgaccgtcg
      421 ctcgcagagc aggagcctca ccgccggcga tcggatcgat ccctccagcc tcgccgcggc
      481 gctgcgcctg tcggaggtgg acccggccgc gctcgggtcc accgctgtcg ccctcgacgt
      541 gcgcctcact gacaacgacg gcttcctgct ggtgtcggtc cagccgagct ggcaagccga
      601 cttcggcctt tacccgaact ccgatttcgg tgagcccgcg gcgctccaga agcgcgttga
      661 ggcacaggtc accgcggtcc gtacgctctt ctcattcgag ggggaggcgc agatgggctg
      721 ggtcgatgct cgcgaccctc ggagggtata ttggcgcccg tagaacccgc agatcggtcg
      781 agaccggccg gcgcggcgca cgcaggatgc atctccgatg tggcttgccc tgggaggcct
      841 ggccctaggc gtggcgctcg gcctggcgct gaacgtgaac gtcagcccgg acctggcgcg
      901 ctacagcgcg gtcggcatcc tggccgggct ggactcggtg ctcggcgcga tccgggcgga
      961 gctcgacgcg cactacgaca accggatctt catctccggc ttcgtgacca acgccctggt
     1021 ggccgcggcc ctcaccttca tcggcgaccg gctggggatc gatctgtacc tggtggcgct
     1081 gttcgccttc gggctgcgca tcttccaaaa cgtggcactc attcgccgcc acttcctgta
     1141 ggctcggacg cacccgtgcc gcggaagcgc ggcgggacct acgggaggta gcttggacag
     1201 ggagacggtc ctggtcggca tcgacgccgg cacgaccaag gtgacgacgc tcatcggcga
     1261 ggtcagccgc ggcggcgacg tcaacatcat cggctatggg atcgcccctt cggtcgggat
     1321 gaagaagggg atggtcgcca acatcgacca gaccgtgaac tccatcgccg ccagcctgga
     1381 gaaggccgaa cgcctgtcgg gctacaagat cagctctgcc ttcgtcgcgg tcgggggcag
     1441 ccacatcagc agccagaaca gccgtggcgt ggtggcggtc tccggccaca agcgcgaggt
     1501 gagccgcgag gatgtggcgc gcgctaccga ggcggccaag gcgatccagg ttccctccaa
     1561 ccgcgagatc ctgcatgtca tcccgcgcgg ctacatcgtc gacggccagg aggggatcaa
     1621 ggacccgctc gggatgagcg cggttcggct ggaggtcgag acccacatcg tggccggcgc
     1681 agccacctcc attcagaacc tgtcgaagtg catcgccaac gccaatgtcg agatcgacga
     1741 gctggtgatc agctcgctgg ccgccgcgga ggcgacgctc accgacacgg agaaggagtt
     1801 gggcgtcatg atcgccgaca tcggcggcgg cacgacggac ctggccatct tcactgatgg
     1861 cgccgtctat cactcggccg tgctgccggt gggcgccatc aacgtcacca acgacgtggc
     1921 gatcggactc cgcaccagcc tcgacctggc ggaggagatc aagatcaagc acggctccgc
     1981 caacctggcc gatatcgcgc cggaggagct gctcaacgtg gcggtcctgg gcgagggcgg
     2041 cgggcagacc atccagcgca agaagctgtg cgagatcatc gaggcccgca tgagcgagat
     2101 cttttcgctc atcggcgagg agatcaagcg ctcgggtcac gccggcctgc tgccggcggg
     2161 cgtggtgctg acgggtggcg gtgcgcggct cgccggggtc gccgagctgg cccgcgaggt
     2221 gctccagatg ccggtccggg tggcatcgcc gcagggcgtc ggcgggctga tggaccagct
     2281 ctccaacccg gccttcagca cgccgatcgg gttgctgctg tggggcgccc accatatcgg
     2341 cagcgagccg atcagcttca cggtcgggtc gccgatgggt ggcggcgtcg gtcgcttcat
     2401 ggactggctg cgcggcctct tccccggctg atcgtcggcg ggccggaact ccgtgatgtg
     2461 gagcaatcgc caatccctcc accgtaattt cgacagctgt ggacaactcg gcgggcgtat
     2521 gtggataaat cggcaacgcc gcatgtgccg ggtcgttaga atcggtcaga cccgcgcgag
     2581 ctccgggttc gcccagcaag cagccagcac ccttgtgcag gagaacccct catgccgctc
     2641 cgttcggacg ccgagcactt cgcactgatc aaggtcatcg gagtcggtgg cggcgggtcg
     2701 aatgccgtca accggatgat tcgcgccgag atgatgggcg tcgaattcat ctcggtgaac
     2761 acggacgcgc aggccctcct gcagagcgac gccccgcaca agatccggat cggcgacaag
     2821 ctgacccgcg ggctcggcgc tggcgcggac ccgatggttg gccagcgcgc agccgaggag
     2881 gacagcgaga agatctacga ggcgctcaag gacgccgaca tgatcttcat caccgccggc
     2941 atgggcggtg gcactggctc cggggcggcc cacgtcatcg ccgagatcgc gaaggacctt
     3001 ggcgcgctga cggtcggcgt catcaccaag ccattcagct tcgagggggt ccggcgcaag
     3061 ctggttgccg agcagttcag cgagctgctc aaggagaagt gcgacaccct gatcaccatc
     3121 ccgaacgatc gcctgcgcga ggtggtcgac aagaagacca gcatcctcga cgccttccgc
     3181 gtggtcgacg acgtgctgcg ccagggcgtg caggggatca gcgacctgat aaccgtgccg
     3241 ggcctcatca acctcgactt cgccgatgtc aagacgatca tgcgcgacgc cgggtcgagc
     3301 atgatgggga tcggcatcgg caccggcgac aaccgcgcgg tcgaggccgc ccgcgccgcg
     3361 gtcatgagcc cgctcctcga ggtcaacatc accggggcac gcgggatcct gttcaatgtc
     3421 accggcggca gcgaccttgg cctgttcgag gtcaacgagg cagccgaggt gatcaaggag
     3481 gccgccgatc cggaggccaa catcatcttc ggcaccgtga tcgacgaccg gatgcgggac
     3541 gaggtcaagg tgacggtcat cgccaccggc ttcgacgcga cccgcaagcc gcgaccggcc
     3601 gcccgagccg tcacccccgc gagcgaggtc gcgaccggca tcgaggcgtc gctggacgag
     3661 cggagccgcg agatcctggc cgagatcgag cgcgaccggg ccgagcgcca ggcgctggag
     3721 gaccagatgc ccgaggcttc gcctttcgcc gttggggaga agccgtccga gaccaccgcc
     3781 cgcaccggcg agcggcaggc gatcccggtg cgtggcagcg ggcttgatcg ccccacctac
     3841 ggggagaccg acctcgacat cccgtccttc ctccggcgca agagcgaata gggccggcaa
     3901 tggaccgatg cgccccggcc gtccggccgg ggcgttcgca tctcctcgac actccgccgc
     3961 gatgctgccc gtcgacgagc cgccactcac cgcggagctg atccgctccc gtcacgacgc
     4021 gctgctgcga cggttgtgcg cggcggccga ggcggccggc cggaatccgg acgccgtacg
     4081 cctggtggcg gtcaccaagg gattcgggac cgccgtcatt cgcgacgcga ccgccgccgg
     4141 gctgagccgc ttcggcgaga accgcgtgca ggaggcgatc cccaagatga gcgcggcgcc
     4201 cgaagccgag tggcacctgg tcgggcgcct gcaggccaac aaggccaggc cggcggtgcg
     4261 cgggttcgcc gccatccact cggtcgactc actcgagctg ctgggtcgcc tcgaccggat
     4321 cgccgccgag gagtcgaggg cgccgattgc attgctccag gtcaacgtga cggcggagac
     4381 gagcaaggca ggcttcgcgc tcgactggtt cgaggaggcg gtcgggcgcc cgggatccct
     4441 gtcggctgca attgccggcc tgaccgctgt gcggttggcg ggattgatga cgatcgggcc
     4501 ggccggggcc gcgaccgctg agtcgcggcg ggccttcgcc acgctgcgag ggctgaggga
     4561 ccagctccag cagctgaccg ggcacccgct gcccgagctg tcgatgggga tgactgcgga
     4621 cgccgatgcg gccgtggccg agggagcgac cctcgttcgg atcggtacgg ccatcttcgg
     4681 tccgcggccc gcctgagcac gcgctgctat cctcgaagtt gagatgccgc tcgtcatcgt
     4741 caacttcctg gagctccttg tcagcgcgct gatgctgctc gtgctggcac gggtcgtcgt
     4801 cagctggctc gcccccaccg ggggcggcgg agtggtcgcg ttcatctacc aggcaactga
     4861 gccgatcctg gcgccgatcc gacgggtggt gccccccagc ggggggctcg actgggcgcc
     4921 gctcatcgcg atgctggtgc ttgggctgct gctccggctg gtggttcgcc tctaggcccc
     4981 ggcgattgag ggtcgtgaag cccatcggta tactgcgaac cccgcgtggg cgattggctc
     5041 agcccggtta gagcgcacgg ttcacatccg tgaggtcact ggttcgaatc cagtatcgcc
     5101 caccaccttt tccctgctgg ggctgatcga tgccgctgct cctccgatct ctgcacgccc
     5161 gaatcctgcg catgcggctc ctggagccgc tgcaggagcg cgacttcgcg ctgctgactg
     5221 ccggatcgct cgtttccctg ctgggcgatg gcttcttcag cgtggcgctg gcgtggcagg
     5281 tgtacgagat cagcaatctg cccaccgccc tctccatcgt cggggtggcg tggaccctgc
     5341 cggtggtcct cttcgtcctg ctcgg
//
LOCUS       DATCQR010000038         7384 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_94711, whole genome shotgun sequence.
ACCESSION   DATCQR010000038 DATCQR010000000
VERSION     DATCQR010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 7384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7384
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94711"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..529
                     /gene="atpH"
                     /locus_tag="VIN32_01325"
     CDS             <1..529
                     /gene="atpH"
                     /locus_tag="VIN32_01325"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HEY8921246.1"
                     /translation="DTSARRYAEAAFEIGRADGTLDAWERDMATIGATMRHPELRSLL
                     QHPAVPFADKERVLRRVMGRGVAAAPINLVLLMVRRGRPGAIDRMIERFTELLRRHRG
                     IALAEVRTALPLDDGQRAELASRLRALTGSEIEMDETVDPDLIGGIAVRIGDELYDAS
                     VRSRLERLRARLTTA"
     gene            546..2036
                     /gene="atpA"
                     /locus_tag="VIN32_01330"
                     /pseudo
     CDS             546..2036
                     /gene="atpA"
                     /locus_tag="VIN32_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393857.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
     gene            2495..3385
                     /gene="atpG"
                     /locus_tag="VIN32_01335"
     CDS             2495..3385
                     /gene="atpG"
                     /locus_tag="VIN32_01335"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HEY8921247.1"
                     /translation="MPSLKDINRRIGSVRNIAQITRAMEMVAASRMKRAQDSILAARP
                     YADELEDALRRVAAAEGLTEEVNALLARRAVRRVAMIVITTDRGLAGSLNSNATRAVL
                     RWVTERASGTNDDRRVEIEAITVGRKGRDALRRAGVPIAAHFAQLGDRPSFADVTPIA
                     RLVTQDFLAEKYDEIDIAYSTFVSTLAQKPKIATLLPVERPEMGEEHQRTNDQYLFEP
                     SPEAVLSRLLPHYVAIGIYRAVLENNASEQSARMIAMRSSTDNANELIDDLTLVYNKT
                     RQATITREMIEIASGAEALG"
     gene            3406..4818
                     /gene="atpD"
                     /locus_tag="VIN32_01340"
     CDS             3406..4818
                     /gene="atpD"
                     /locus_tag="VIN32_01340"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007506142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEY8921248.1"
                     /translation="MATGKVIQITGPVIDVEFPPGELPSIYNALEIKRPKKAGQTGDD
                     ATLVVEVQQHLGNNWVRAVAMSTTDGLARGLDVVDTGAPITVPVGEATLGRVFDVLGH
                     PIDGKGPVKAEKSLPIHRDPPPFDQMETEAQVFETGIKVIDLIAPFRRGGKVGVFGGA
                     GTGKTVIIQELIRNIAAEHGGYSVFAGVGERSREGNDLLGEMTESGVIDKTVMVFGQM
                     NEPPGARQRVGLTALAMAEYFRDEEGRDILLFIDNIFRFTQAGSEVSALLGRMPSAVG
                     YQPTLATEMGDLQERITSTKKGSITSLQAIFVPADDYTDPAPATTFAHLDSTIRLERS
                     IVELGIYPAVDPLTSTSTVLDRRIVGDEHFEVSREVQRTLQRYKDLQDIIAILGMDEL
                     SEEDKVSVSRARRLQRFMSQPMFVAEQFTGRPGAYVELKETVRGFREILDGKHDQLPE
                     QAFFLAGTIDDVAANAEKMG"
     gene            4818..5228
                     /locus_tag="VIN32_01345"
     CDS             4818..5228
                     /locus_tag="VIN32_01345"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010936341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEY8921249.1"
                     /translation="MPLKLEIVSPERMVYDDEVDMVIVPGRNGQLGILPHHTPLISSL
                     GVGELRIKKGGSEESMLISGGFVEVRPDKVIVMADLAEHSDEIDEAKAVEARKRAESE
                     LEQSRDPVDIARVRAALQTALMRERIATRRRSPG"
     gene            5228..5551
                     /locus_tag="VIN32_01350"
     CDS             5228..5551
                     /locus_tag="VIN32_01350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921250.1"
                     /translation="MQPCDQCGSRFAQLPVRIEIVRESGPIGLPGDSARTSSNFSKGY
                     AFCSSDHAYAWARERRYMLCNAAPTPFEEGMQPFLPSRHMGGRVGLCDGHHLGPHEWK
                     EAWPA"
     gene            5565..6836
                     /gene="murA"
                     /locus_tag="VIN32_01355"
     CDS             5565..6836
                     /gene="murA"
                     /locus_tag="VIN32_01355"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF006873.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEY8921251.1"
                     /translation="MQKFIVTGGVPLRGEVRIAGAKNAVLKMMAAAVLTDDRCVLRNV
                     PRISDVQILRGAMTDIGFSVSPLEDGALEITATGADWLFVPLEAAMKMRSSFILLGPL
                     LSRFGRVIISNPGGDRIGRRPVDLHVDAMRSLGAEIEYKNGYYFAQAPGGLRGARITF
                     PYVTVMGTENAMLAGTLARGTTVIENAAQEPEVDDLVAMLRAMGASIERTAPHRLEIE
                     GVDRLHGVEHRVLGDRLEAGTFAMAAAVTGGEITVRGVDPTHLAAFTDLLTGMGISSD
                     TFTEDGGGLRVRGGDAIRPADVETQPYPGFATDLQAPLAVLMTQAQGISTIHETIYED
                     RLDYTMELVKMGAVIEVLDERHARIAGPTPLHGREVEIADLRAGATLILAALAADETS
                     VISGVEHVDRGYEAIESKLVNLGAQISRIDA"
     gene            6885..>7384
                     /locus_tag="VIN32_01360"
     CDS             6885..>7384
                     /locus_tag="VIN32_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEY8921252.1"
                     /translation="MKIGIDLGTANILVYVQGKGVVLNEPSVVAINDDDNRLVAVGEE
                     AREMIGRTPGNVRAIRPMRDGVIADYLITEAMLRYVINKVSRVRLFKPDVMISVPSGV
                     TSVEKRAVRDAAMKAGARDAYLIDEPLAAAIGAGVPISGPSGNMIVDIGGGTTEIAVI
                     ALGGIVV"
BASE COUNT         1339 a         2468 c         2520 g         1057 t
ORIGIN      
        1 cgacacgtcc gcccggcgct acgcggaggc ggccttcgag atcggtcgcg ccgacgggac
       61 gctggacgca tgggagcggg acatggcgac gatcggcgcg acgatgcgcc atccggagct
      121 gcgaagcctg ctccagcatc ccgcggttcc cttcgcagac aaggagcgag tgctgcgacg
      181 cgtgatgggg cgaggcgtcg ctgcggcgcc gatcaacctc gtgctgctga tggtccggcg
      241 gggccgtccc ggggcgatcg accgcatgat cgagcgcttc acggagctcc tgcggcgaca
      301 ccgcgggatt gcgctggccg aggtgcggac cgcgctgcca ctcgacgacg ggcagcgcgc
      361 agagctcgcc tcgcggttgc gtgcactcac cgggtcggag attgagatgg atgagacggt
      421 cgatccggac ctgatcggcg gcatcgccgt gcggatcggc gacgagctct atgacgcaag
      481 cgtgcggagc cgccttgagc ggctgcgcgc gcgactgacc acggcttagg agtacgaggg
      541 agttcatgac gatccgatcc gacgagatca cgtcgatcat tcgcagcgag atcgatgccc
      601 tggacgaagg cgtggacgtc agtggcgtcg gcacgatcgt cgaggtgggc gatggcatcg
      661 cccaggtcta cggcctgtcc ggcgccctcg cgcaggagct cctcgacttc ggcaacggga
      721 tcatgggcat ggccttcaac ctggaggagg agaccgtcgg cgccctgatc ctgggcgact
      781 acacctcact caaggagggc gaccaggtca agacgaccgg tcgcatcgtt gaggtgccgg
      841 tgggcgacgc actgatcggc cgggtggtga accccctcgg ggagcccctc gacggcaagg
      901 gcaagatcga gacgaccagg acgcgcccga tcgagcgcat tgcccccggc gtcatcgtgc
      961 ggcagccggt cgatacgccg gtgcagaccg gcatcaaggc gatcgactcg atgatcccga
     1021 tcggccgcgg acagcgcgag ctgatcatcg gcgaccgcca gaccgggaag accgccatcg
     1081 cggttgacac gatcatcaac cagaaggggc aggacctgat ctgcatctat gtcgccatcg
     1141 gccagaagca gtcgaccgtg gcacaggtgg tcgcgcagct tgaggaatcg ggcgccatgg
     1201 accacaccat cgtggtcttc gccggcgcca gcgacccggc cccgctgcag tacctggccc
     1261 cctacgccgg cgtgacgatg ggggaggagt tcatggacgc gggtcgtgac gccctgtgcg
     1321 tgtacgacga cctctccaag cattcctggg cataccgcca ggtctcactg ctcatgcgac
     1381 gccctcccgg tcgcgaggcg ttccccggcg acatcttcta ctcccactcc cggctgctcg
     1441 agcgggccgc gcggatgaac aagaagaacg gcggcggatc cctgaccgca ctgccgatca
     1501 tcgagacgca ggcaggcgac gtttccgcct acatccccac gaacgtgatc agcatcaccg
     1561 acggccagat cttcctgcag acggacctct tcaaccaggg ccagcgcccc gcggtaaatg
     1621 ccgggatcag cgtctcgcgg gtcgggggcg cggcgcagat caaggcgatg cgccaggtcg
     1681 cgggcaagct gcggatcgac cttgcgcagt accgcgagct cgccgccttc gcccagttca
     1741 gctcggacct cgacaaggcg actcgcgacc agctcacccg cggcgagaag acgacggaga
     1801 tcctgaagca gccacagtac gagccacttc ccgtggagaa gcaggtcgtc atcatctggg
     1861 ccgccaccaa cgggctgctc gacgacgtcc ccaccggcaa gatcgccgag ttcgagaccg
     1921 gcctctatcg ccaccttgaa tccggctacg agaaggtgct gccgacgatc gcgaaggaga
     1981 agctgctctc cgacgcgacg attgcgacgc tcgagaaggc ggtcgccgag ttcaagcgcg
     2041 agggtgggta cggcgagacc gatgacgctc ccccgacagc gacgggcgag gccgcgcaga
     2101 cggctgagac cggcagcgcg gacgaatcgg cgaaggacga atcggcgaaa gacgaatccg
     2161 cggaggagcg accggcggag aagaagcagc cagccgcagc gagagccaag gcatcggcaa
     2221 gagccaaggc gcccgcgaag cccagggcta gtgcggccga gaccccaaag gttgaggaaa
     2281 ccgatgcggt ggccacggcg aaggcgaagg caaaggcagg cgctaaggag gacgtcgccg
     2341 aggagcggct cgaagaatcg atccgcacca tgaagaagcc tggggagaag tcctgatgac
     2401 gctcctcagc gtccatggcg gtcgcgacgt atatgcgtat acaccgtatt tacgcgacgg
     2461 ctcgacgcat catcgcgagg cgcggttctg acagatgcca agcctgaagg acatcaaccg
     2521 ccggatcggg tcggtccgga acatcgccca gatcacgcgg gcgatggaga tggtggccgc
     2581 gtcccgcatg aagcgggcac aggactccat cctcgcggca cgtccatacg ccgacgagct
     2641 ggaggatgcc cttcgacgcg tcgccgccgc cgaaggcctg acggaggagg tcaatgcgct
     2701 tctcgcgcgt cgcgcggtgc gccgagtcgc gatgatcgtg atcacgaccg accgtggcct
     2761 ggctggctcg ctcaactcga atgccacacg cgcggtcctg cgctgggtca ccgagcgcgc
     2821 ctccgggacc aacgacgacc ggagggtcga gatcgaggca atcaccgtcg gccgcaaggg
     2881 acgcgatgcg ctgcgccgcg cgggagtgcc gatcgccgcg catttcgcgc agcttggcga
     2941 ccggccgtcg ttcgccgacg tgacgccgat tgcgcgcctg gtgacccagg acttcctggc
     3001 ggagaagtac gacgagatcg atatcgccta cagcacgttc gtgtcgacgc tcgcccagaa
     3061 gcccaagatc gccacgctcc tgccggttga gcgacccgag atgggcgagg agcaccagcg
     3121 gacgaacgac cagtacctgt tcgagccgtc gccggaggcc gtgctgagcc gcctcctgcc
     3181 gcactacgtg gcgatcggca tctatcgagc ggtcctcgag aacaacgcat ccgagcagtc
     3241 ggcgcggatg atcgcgatgc gcagttcgac cgacaacgcg aacgagctca tcgacgacct
     3301 gacgctcgtc tacaacaaga cgcgccaggc caccatcacc cgcgagatga tcgagatcgc
     3361 ctccggtgcg gaagcgctcg gctaggcaag gagagagaca gaccgatggc gaccggtaag
     3421 gtgatccaga tcacgggccc cgtgatcgac gtcgagtttc cgcccggcga gctgcccagc
     3481 atctacaacg cgctcgagat caagcgcccg aagaaggcag gccagacggg cgacgacgcg
     3541 acgcttgtgg tcgaggtcca gcagcacctg ggcaacaact gggtgcgggc agtcgcgatg
     3601 tccacgaccg atgggctggc gcgcgggctg gacgttgtcg ataccggcgc ccctatcacc
     3661 gtgccggtcg gtgaagccac cctcggacgc gtgttcgacg tccttggcca cccgatcgat
     3721 ggcaagggcc ctgtcaaggc ggagaagagc ctccccatcc accgcgaccc gccgccgttc
     3781 gaccagatgg agacggaggc gcaggtcttc gagaccggga tcaaggtcat cgacctgatc
     3841 gcccccttcc ggcgcggcgg caaggtcggt gtcttcggcg gtgccggcac ggggaagacg
     3901 gtcatcatcc aggagctgat ccgcaacatc gccgcggagc acggcggcta ctcggtgttc
     3961 gccggcgtcg gcgagcgctc gcgcgaggga aacgaccttc tcggtgagat gaccgagtcg
     4021 ggggtcatcg acaagacggt catggtcttc ggccagatga acgagccgcc tggcgcgcgt
     4081 cagcgcgtcg gcctgacggc cctcgccatg gccgagtact tccgcgacga ggagggacgc
     4141 gacatcctcc tcttcatcga caacatcttc cggttcacac aggccggctc cgaggtgtcc
     4201 gcgctccttg gccgcatgcc ttcggcggtg ggctaccagc cgacgctggc caccgagatg
     4261 ggagacctcc aggagcgcat cacctcgacc aagaagggct cgatcacgtc cctgcaggcc
     4321 atcttcgtgc ccgccgacga ctacaccgat ccggcgcccg cgaccacctt cgcccacctc
     4381 gattccacca ttcgccttga gcgctcgatc gtggagctgg gcatctaccc cgccgtcgac
     4441 ccgctgacct cgacatcgac cgtgctcgat cggcgcatcg tgggggatga gcacttcgag
     4501 gtctcgcgag aggtgcagcg aactctgcag cgctacaagg acctccagga catcatcgcc
     4561 atcctcggca tggacgagct gtcggaggag gacaaggtgt ccgtgtcgcg cgcgcgacgg
     4621 ctgcagcgct tcatgagcca gccgatgttc gttgctgagc agttcacggg ccgccccggc
     4681 gcatacgtgg agctcaagga gacggtccgc ggattccgcg agatcctcga cggcaagcac
     4741 gaccagttgc cggagcaggc cttcttcctg gccggcacga tcgacgacgt agccgcgaac
     4801 gcggagaaga tgggctgatg cccctcaagc tggagatcgt cagccctgag cgcatggtgt
     4861 acgacgacga ggtcgacatg gtcatcgtgc ctggccgcaa cgggcagctc ggcatcctgc
     4921 cgcaccacac gccgctcatc agctcgctcg gtgtcggcga gctgcggatc aagaagggcg
     4981 ggagcgagga gtcgatgctc atcagcggag ggttcgtgga ggtgcggccg gacaaggtca
     5041 tcgtgatggc cgacctggcg gagcactccg acgagatcga cgaggcgaag gccgtcgagg
     5101 cgcgcaagcg cgccgagtct gagctggagc agtccaggga cccggtcgac atcgcccgcg
     5161 tgcgcgccgc gctgcagacc gccctgatgc gagagcggat cgccacgcgg cgacgctcgc
     5221 cgggctgatg caaccctgcg atcagtgcgg ctcccggttc gcgcagctgc cggtacggat
     5281 cgagatcgtc cgcgagtctg gaccgatcgg gctgccaggc gactcggcgc gaacctcctc
     5341 gaacttcagc aaggggtacg ccttctgctc cagcgatcac gcctacgcat gggcgcgcga
     5401 gcgccgctac atgctctgca acgcggcccc gaccccgttc gaggaaggca tgcaaccatt
     5461 cctgccctcg cgacacatgg gcggtcgcgt gggcctctgc gacggccatc acctcggccc
     5521 gcacgagtgg aaggaggcgt ggcccgccta gcgcgggtcg gctgatgcag aagttcatcg
     5581 tcacaggtgg cgtaccgctc cgcggcgagg tgcgcatcgc cggcgccaag aacgcggtcc
     5641 tcaagatgat ggccgccgcc gtgctgaccg acgatcgctg cgtcctgcgc aacgtgccgc
     5701 ggatcagcga cgtgcagatc ctgcgggggg cgatgacgga catcggcttc tcggtcagtc
     5761 cgctcgagga cggcgccctc gagatcaccg ccacgggagc cgattggctg ttcgtcccac
     5821 tcgaggcggc catgaagatg cgctcgtcat tcatcctgct tgggccgctc ctcagccgct
     5881 tcgggcgcgt catcatcagc aaccccggcg gcgatcggat cggcaggcgg ccggtggacc
     5941 tgcacgtcga cgccatgcgc tccctcgggg cggagatcga gtacaagaac gggtactact
     6001 tcgcgcaggc cccgggcggg ctgcggggcg cgcggatcac cttcccttac gtgacggtga
     6061 tggggacaga gaacgcgatg cttgccggga ccctggcccg cggcacgacg gtcatcgaga
     6121 atgcggcgca ggaacccgag gtcgacgacc tggtggcgat gctgcgggcc atgggcgcca
     6181 gcatcgagcg gaccgcgccg catcggctgg agatcgaagg cgtcgaccgg ctgcatgggg
     6241 tcgagcaccg cgtcctcggc gaccggctgg aggcgggcac cttcgccatg gccgcggccg
     6301 tgaccggcgg cgagatcacg gttcgtggcg tcgaccccac ccaccttgcg gccttcactg
     6361 acctgctgac cggcatgggc atctcgagcg acaccttcac cgaggacggt ggcgggcttc
     6421 gcgtgcgcgg cggcgacgcg atccgaccgg ccgatgtcga gacgcagcca tacccgggct
     6481 ttgcgaccga tctccaggcc ccgctggccg tgctcatgac ccaggcccag ggcatctcga
     6541 ccatccacga gacgatctac gaggaccgcc tcgactacac catggagctg gtcaagatgg
     6601 gcgccgtgat cgaggtgctt gacgagcgcc acgcgcgcat cgccggcccg acccccctgc
     6661 acggccgcga ggtggagatc gccgacctgc gggccggcgc aacgctcatc ctggcggcgc
     6721 tggcggccga cgagacgagc gtgatctcgg gagtcgagca cgtcgaccgt ggctatgagg
     6781 cgatcgagtc caagctcgtc aacctgggcg cccagatcag ccgcatcgac gcctgaggcc
     6841 cgtggctgcc gcgatggcgc gcctccggtc acaatgaacg gagcatgaag atcgggatcg
     6901 acctcggaac ggccaacatc cttgtctacg tgcagggcaa gggggtcgtc ctcaacgagc
     6961 cgagcgtggt cgccatcaac gacgacgaca accgcctcgt ggccgttggc gaggaggcac
     7021 gcgagatgat cggccggacc cccggcaacg tccgggccat ccgtccgatg cgcgacggag
     7081 tgatcgccga ctacctgatc acggaggcga tgctgcgcta cgtcatcaac aaggtctccc
     7141 gcgtccggct cttcaagccg gacgtcatga tcagcgtccc gtccggggtc accagcgtcg
     7201 agaagcgcgc ggtgcgcgac gcggccatga aggccggcgc acgcgacgcc tacctgatcg
     7261 acgagccact agccgccgcc atcggtgccg gcgtcccgat ctcgggaccc tcgggaaaca
     7321 tgatcgtcga catcggcggc ggcaccaccg agatcgcggt gatcgccctg ggcggcatcg
     7381 tggt
//
LOCUS       DATCQR010000039         3054 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_125953, whole genome shotgun sequence.
ACCESSION   DATCQR010000039 DATCQR010000000
VERSION     DATCQR010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3054
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125953"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="VIN32_01365"
     CDS             complement(<1..189)
                     /locus_tag="VIN32_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921253.1"
                     /translation="MGLVARRAFGFSVGFSAFGSAAAAFFVLGAAAAAFLVLGLAVRG
                     AAFFSPLAGAPAAASLASA"
     gene            215..484
                     /gene="rpmA"
                     /locus_tag="VIN32_01370"
     CDS             215..484
                     /gene="rpmA"
                     /locus_tag="VIN32_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014808297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEY8921254.1"
                     /translation="MAHKKAGSSSRNGRDSAGQRLGVKRGDGQQVHAGTIIVRQRGST
                     FNPGENVGMGRDYTLFATSEGRVKFSHETRHKKRVSVVSSETSAA"
     gene            512..724
                     /gene="rpmE"
                     /locus_tag="VIN32_01375"
     CDS             512..724
                     /gene="rpmE"
                     /locus_tag="VIN32_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012173900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HEY8921255.1"
                     /translation="MKADIHPQYHQAQVHCVCGTSFTVGSTVESIRVEVCANCHPFYT
                     GTQNIVDTAGQVERFQRRMERSQRES"
     gene            726..1757
                     /locus_tag="VIN32_01380"
     CDS             726..1757
                     /locus_tag="VIN32_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012818218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HEY8921256.1"
                     /translation="MRRHLTGWRGRLAPHLLFLVPELLAAAGPFVIASIARAGERPRF
                     RMPDFFYGGQALIEGVMMRGRTTVAMSVRPPSGEIRTYSEPLPGAMARGRWVKVPFAR
                     GVLVLYEALVIGTRMLMRSAAIAAEGEDIQIGRGTMALTMAISIGFAVGLFFILPLFL
                     STFAERATTSDLLANLAEGGIRLGVFIAYIWLIGRMGEVRRVFAYHGAEHKAISAHEA
                     ERPLTPDEVDRFSTAHTRCGTTFLLIVVVISILFFGLIPRAGVPLPLLFLSRIVLVPV
                     IAAFAYELVRFGASHYGNPVVRAIYAPGLWLQALTTRPPDHGMLEVSIASLESCLASD
                     REAAAPTSP"
     gene            1754..2833
                     /gene="prfA"
                     /locus_tag="VIN32_01385"
     CDS             1754..2833
                     /gene="prfA"
                     /locus_tag="VIN32_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007049686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEY8921257.1"
                     /translation="MIDRLRQIEASYDAVVAEMATPAAANNAARLQALGRELARLEPM
                     VEAIRAWSAVRDELEATRSMAHDPDEEVRAMAREELTALETREQAIDADLRRLLVPRD
                     PNDDRDVILEVRAGTGGDEAALFAGDLYRMYARYAERRRWKFDLISVSEGGGGGVKEA
                     IAEIAGQGAYSRLKFESGVHRVQRVPTTEAAGRIHTSTATVSILPEADEVEIQIPDKD
                     LRIDVYRSSGPGGQSVNTTDSAVRVTHLPSGLVVAIQDEKSQHKNKAKALAVLRARLL
                     ELEQARQAEERGDQRRSQVGSGERAEKIRTYNFPDDRVTDHRVGITVHNLPGLLEGDL
                     DRLIEPLAEADQAARLAQIGADDSP"
     gene            2838..>3054
                     /locus_tag="VIN32_01390"
     CDS             2838..>3054
                     /locus_tag="VIN32_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011506829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEY8921258.1"
                     /translation="MPAAVLPQASAGRLVADTARSLQASGIPSPRLDAELLVGHAFGR
                     DRAWLHAHPDAELGPGEARLLAGWVERR"
BASE COUNT          498 a         1035 c         1092 g          429 t
ORIGIN      
        1 ggcggacgcg agcgaggccg ctgccggggc gccggccaag ggggagaaga aggcggcgcc
       61 ccggaccgcc aggccgagga cgaggaaggc agctgctgcc gcgccgagga cgaagaaggc
      121 agcggctgcc gagccgaagg cggagaagcc gaccgagaag ccgaaggccc gccgggcaac
      181 caggcccaag ccgaaggcag aggagtagcg agcgatggca cacaagaagg caggcagctc
      241 gagccgcaac ggtcgcgact ccgccgggca gcggctcggc gtgaagcgcg gcgacggcca
      301 gcaggtgcac gccggtacga tcatcgttcg ccagcggggg agcacattca acccggggga
      361 aaacgtcggc atgggccgtg actacaccct cttcgcgacg agcgagggga gagtcaagtt
      421 cagccacgaa acgcgtcaca agaagcgcgt cagcgtcgtc agttcagaga catcagcggc
      481 ctaggggccg cgggatccca ggagccaagc catgaaggcc gatattcatc cgcagtacca
      541 tcaggcgcag gtccactgcg tctgcgggac ctcgttcacg gtcggctcga ccgtggagtc
      601 gatccgcgtc gaggtctgcg ccaattgcca cccgttctac accgggaccc agaacatcgt
      661 cgacaccgcc ggccaggtcg agcggttcca gcgacgcatg gagcgatcgc agcgcgagtc
      721 gtgacttgcg ccgccacctg acagggtggc gggggcgcct ggcgccccat ctcctcttcc
      781 tcgttcccga gctgctggct gctgccggtc ccttcgtgat cgcctcgatt gcgcgcgccg
      841 gcgagcggcc acgctttcgc atgccggact tcttctacgg cggccaggcg ctgatcgagg
      901 gggtcatgat gcgcggccgc acgaccgtgg ccatgagcgt ccgcccgccg tccggcgaga
      961 tccggaccta cagcgagcca ctccccgggg cgatggctcg tggccggtgg gtcaaggtgc
     1021 cgttcgcgcg cggcgtgctc gtgctgtacg aggcgctcgt gatcggaacc cgcatgctga
     1081 tgcgctcggc cgcgatcgcg gctgagggcg aggacatcca gatcggcagg ggcacgatgg
     1141 cgctgacgat ggcgatctcg atcggcttcg ccgtcggcct cttcttcatc ctgccgctct
     1201 tcctgtcgac gttcgcggag cgagccacga ccagcgacct gctggccaac ctggcggagg
     1261 gcgggattcg cctcggcgtc ttcatcgcat acatctggct gatcgggcgc atgggggaag
     1321 tccgtcgcgt gttcgcgtac cacggtgcgg agcacaaggc gatcagcgcg catgaggcgg
     1381 agcgcccgct cacgcctgac gaggtcgatc ggttcagcac cgctcacacg cgctgcggca
     1441 cgaccttcct cctgatcgtg gtcgtcatca gcatcctctt cttcggcctg atcccgcggg
     1501 ccggcgtgcc tctcccgctc ctcttcctgt ctcggatcgt gctggtgccc gtgatcgctg
     1561 ccttcgcgta cgagctcgtg cggttcgggg cgagccatta cggaaatccc gtcgtgcgcg
     1621 cgatctacgc cccggggctc tggctccagg cgctcaccac ccgacccccg gaccacggca
     1681 tgctggaggt gagcatcgcc tccctcgagt cgtgcctggc gtcggatcgc gaggcggcag
     1741 ctccgacgag cccatgatcg accgcctgcg gcagatcgaa gcgagctatg acgcggttgt
     1801 ggcagagatg gcgacaccgg cggcggccaa caatgcggcg cgcctgcagg cgctgggccg
     1861 cgagctcgct cggctcgagc cgatggtgga ggccatccgc gcatggtccg ccgtgaggga
     1921 cgagctggag gcgacgcgct ccatggcgca cgaccccgac gaggaggtgc gtgcgatggc
     1981 acgcgaggag ctgaccgccc tggagactcg cgagcaggcg atcgacgccg acctgcgccg
     2041 gctgctggtg ccccgcgacc cgaacgacga tcgagacgtg atcctcgagg ttcgtgccgg
     2101 caccggcggc gatgaggcgg cgctcttcgc cggcgacctg taccgcatgt acgcgcgcta
     2161 tgccgagcgg cggcggtgga agttcgacct gatctccgtc agcgagggcg gcggcggtgg
     2221 ggtcaaggag gccatcgcgg agatcgcggg gcaaggcgcc tattcgcgac tgaagttcga
     2281 gagtggggtg caccgcgtgc agcgggtgcc gaccaccgag gcagccggac ggatccacac
     2341 ctcgaccgcc accgtcagca tcctccccga ggccgatgag gtcgagatcc agatccccga
     2401 caaggacctg cgcatcgacg tctaccggtc cagcggcccg ggcggccaga gcgtcaacac
     2461 caccgacagc gcggtgcgcg tcacgcacct gccgagcggg ctggtggtgg cgatccagga
     2521 cgagaagagc cagcacaaga acaaggccaa ggcgctggcg gtgcttcggg ctcgactgct
     2581 ggagctcgag caggctcgac aggccgagga gcgtggcgac cagcgccgat cccaggtcgg
     2641 gagcggcgag cgggcagaga agatccgcac ctacaacttc cccgatgacc gtgtcaccga
     2701 ccatcgagtc ggaatcacag tgcacaacct gcccggcctg ctggagggtg atctcgatcg
     2761 gttgatcgag ccactcgccg aggccgatca ggcggcgcgc cttgcgcaga tcggcgccga
     2821 tgactccccc tgagtccatg ccggcggcag tcttgccgca ggccagcgcc ggccgcctgg
     2881 tggcggacac ggcaaggtcc ctgcaagcga gcggcatccc gtcgccgcgc ctcgatgccg
     2941 agctgctggt ggggcatgct ttcggccgcg accgcgcctg gctgcatgcc caccccgacg
     3001 ccgagctggg ccccggcgag gcgcggctgc tggccggctg ggtcgagcgg cggg
//
LOCUS       DATCQR010000040         3150 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_125964, whole genome shotgun sequence.
ACCESSION   DATCQR010000040 DATCQR010000000
VERSION     DATCQR010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3150
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125964"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="VIN32_01395"
     CDS             complement(<1..257)
                     /locus_tag="VIN32_01395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921259.1"
                     /translation="MDQHAIEHTGDETQAESCRFCGGGLLTSVFDTSFRDTAAEERLF
                     FAIPGALCVPCRQLYVDRQLIGILGLSESQCTFAIQSDRVLR"
     gene            complement(345..659)
                     /gene="rsfS"
                     /locus_tag="VIN32_01400"
     CDS             complement(345..659)
                     /gene="rsfS"
                     /locus_tag="VIN32_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEY8921260.1"
                     /translation="MDLASDKKAVDIVLLRTTEVTTMADYFVIGTGRSDRQLQALASG
                     IIDDLRDEGIRPLGVEGKGAARWVLLDYGAVIVHLFAPEEREFYGLERLWSHAAQVVR
                     LV"
     gene            complement(716..1423)
                     /gene="nadD"
                     /locus_tag="VIN32_01405"
     CDS             complement(716..1423)
                     /gene="nadD"
                     /locus_tag="VIN32_01405"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEY8921261.1"
                     /translation="MVVQAEGSTEPASEQSAAAADPAHRSSRKARARRVGILGGTFDP
                     PHIGHLWLATLAADGLGLGQVLLMPAARPPHKGRRAVSNAADRVMMTRLAITGDPGLD
                     LSLVEMERPGPSYTVDSLVQLRAHLGQDAGLVLIMASDSFAEIETWREPDRLLELAEL
                     AVGPRPGTPLPEPRALRERFGAAAARIHLLEGPSLDLSSSDIRARVAAGRPIRYLVPR
                     AVEELIAARGLYRRMRP"
     gene            complement(1429..2727)
                     /gene="obgE"
                     /locus_tag="VIN32_01410"
     CDS             complement(1429..2727)
                     /gene="obgE"
                     /locus_tag="VIN32_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013074739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEY8921262.1"
                     /translation="MFADQVRIFVAGGAGGDGSASFRREAHVPRGGPDGGDGGSGGSV
                     VLVVDAGMTTLGDLRHRRHHRARPGGRGARRKAHGKNASDVLVPVPPGTVVRMAPAGD
                     ESDPDGALLGELLAPGERLVVGRGGRGGRGNVHFVSATHQAPTHAQKGEPGESQWVEL
                     ELKLIADIGLVGAPNAGKSSLLAAVTAATPEVGDYPFTTIRPNLGVIELDEERRAVIA
                     DLPGLIEGASEGRGLGHAFLRHAERTRVLVAVVDGAAADPLGEWRAVEGELREHDPSL
                     MERPMPMVVTKQDLPEVHAAWPAIRRTLRATDVKPIAVSAHAGTGLPELRTALAEALD
                     EAQRRSAAEPPPGAVRLHRFDPLDAGWQVIAEADGLRVQGRRIETTAARTDFENEESR
                     DRFQRTLERMGIDAELRRLGARSGTMVRIGSVELEWGDED"
     gene            complement(2802..>3150)
                     /locus_tag="VIN32_01415"
     CDS             complement(2802..>3150)
                     /locus_tag="VIN32_01415"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEY8921263.1"
                     /translation="LWPVPGGVITQYYSSYHRALDIAAPRGSKVIAADAGIVTWSGWK
                     NNGGGLVIAIDHGNGIVTIYNHLGSIWVAAGTAVAAGQGIAAVGCTGVCTGPHVHFEV
                     IVGGVIVNPLRYL"
BASE COUNT          476 a         1118 c         1093 g          463 t
ORIGIN      
        1 cgcaggaccc gatcgctctg gatggcgaag gtgcactgcg actcggagag gccaaggatc
       61 ccgatcagct gacggtcgac gtacaactgg cggcacggca cgcacaaagc cccgggaatg
      121 gcgaagaaga gtcgctcctc agcggcggtg tcgcggaacg aggtgtcgaa gacgctggtc
      181 agcagcccgc cgccgcagaa gcggcatgac tcggcctgtg tctcatcgcc cgtgtgctcg
      241 atggcgtgct ggtccatggg ccgagcatgg aaggggggcc cgatcggctc aataggaggt
      301 ccggcgtggc tggactggta cccgggtagt acctgcggcc gaggtcagac caggcggacg
      361 acctgggcgg cgtggctcca cagccgttcg aggccgtaga actcgcgctc ctcgggggcg
      421 aagagatgga cgatcacggc gccgtagtcg agcaggaccc agcgtgcggc acccttgccc
      481 tcgaccccca gcgggcggat cccctcgtcg cgcagatcgt cgatgatccc gctggcaagc
      541 gcctgcaact gacggtcgct gcggcccgtg ccgatcacga agtagtcggc catggtcgtc
      601 acctcggtcg tccgcagcag cacgatgtcg accgccttct tgtccgaggc gaggtccacg
      661 atccgatgcg cgaggtcgcc cggatcgatg gccgtcagcc cgtctgcgcg ctcctctatg
      721 gtctcatcct tcgatacagg cctcgggccg cgatcaactc ctccacggcc cgcgggacaa
      781 ggtaacggat ggggcgaccc gccgccaccc tggcgcggat gtcggagctg ctcaggtcga
      841 gcgacgggcc ctcgagcagg tggatgcggg ccgcggctgc accgaagcgc tcgcgaagtg
      901 cgcgaggctc cggcaggggg gtcccgggac gtgggccgac ggccaattcc gccagctcga
      961 gcagccggtc aggctcgcgc caggtctcga tctccgcgaa cgagtccgag gccatgatca
     1021 ggaccagccc ggcgtcctgg ccgagatgag cgcgcagctg caccagtgaa tcgaccgtgt
     1081 aggaaggccc cgggcgttcc atctccacca gggagaggtc gagacccgga tcgccggtga
     1141 tcgccaggcg ggtcatcatc acccggtcgg ccgcgttgct caccgccctg cgacccttgt
     1201 gcgggggtcg ggctgcgggc atcagcagga cctggccgag gccaaggccg tcggcggcca
     1261 gggtggccag ccagaggtgg ccgatgtgag gcgggtcgaa ggtgccaccg aggattccga
     1321 ccctcctggc ccgagccttt cggctggatc ggtgagccgg atcggccgct gccgcgctct
     1381 gctcggaggc gggttccgtc gatccctccg cctgtacgac cacgctcctc aatcctcgtc
     1441 tccccactcg agctcgaccg agccgatgcg caccatcgtg ccgctccgcg ctcccagccg
     1501 tcgcagctcg gcatcgatcc ccatccgttc caaggtgcgc tggaatcggt cgcgcgattc
     1561 ctcgttctcg aaatcggtcc gggcggcggt cgtctcgatg cgccggccct gcacccggag
     1621 gccatcggcc tcggcgatca cctgccatcc ggcgtccagc ggatcgaacc gatgcagacg
     1681 caccgcgccg ggcggtggct ccgcggcgga acggcgctgc gcctcgtcga gcgcctccgc
     1741 cagtgccgtc cggagctcgg gcaggccagt gccggcatgc gcggagacgg cgatcggctt
     1801 gacgtcggtc gcccgcagcg ttcggcggat cgcgggccag gctgcgtgca cctcgggcag
     1861 gtcctgcttg gtgacgacca tcggcatcgg cctttccatc agcgatggat cgtgctcgcg
     1921 gagctcacct tcgacggccc gccattcgcc gaggggatcg gccgcggctc cgtcgaccac
     1981 tgccaccagc acgcgcgtgc gctcagcgtg gcgcagaaag gcgtgcccca gcccacgacc
     2041 ctcggaggca ccctcgatca gcccaggcag gtctgcgatc accgcccggc gctcctcgtc
     2101 gagctcgatc acacccaggt tgggtcggat ggtggtgaac ggatagtcgc cgacctccgg
     2161 cgtggcagcg gtgaccgccg cgaggagcga ggacttcccg gcgttgggtg cgcccaccag
     2221 gccgatgtcg gcgatcagct tcagctcgag ctcgacccac tgcgattcgc ccggctcccc
     2281 cttctgggca tgcgtcggcg cctggtgcgt ggcgctgacg aaatgcacgt tgccacggcc
     2341 gccacggccg ccacggccga ccacgagccg ctcgccggga gcaagcagct cgcccagcag
     2401 ggctccgtca ggatccgatt cgtcgcctgc cggcgccata cgcaccacgg tgccgggtgg
     2461 aacgggcacg agcacgtcgg aggcgttctt gccatgtgcc ttgcggcggg caccgcgacc
     2521 accaggcctg gcccgatggt gccggcgatg gcgcaggtcg ccgagcgtcg tcatcccggc
     2581 atccacgacc agcacgaccg atccgccgga accgccgtcc cctccgtcgg gtccgccgcg
     2641 cggaacgtgg gcttctcgtc gaaacgaggc cgatccgtcg ccgccggcgc cgccggccac
     2701 gaagatccgg acctggtcag cgaacatcgc cgaccgatga tagccacgag actggcgccg
     2761 ccctggccgg gcgacgatga gcgccgcggg agcgaacggg cctagaggta gcggagggga
     2821 ttgacgatga ccccgccgac gatcacctcg aagtggacgt gtggcccggt gcagaccccg
     2881 gtgcagccca ccgcagcgat cccctggccg gccgcgaccg cggtgcccgc ggcgacccat
     2941 atgctgccca ggtggttgta gatggtgacg atcccgttgc cgtgatcgat ggcgatcacc
     3001 agtccgccgc cattgttctt ccagccggac caggtgacga tgccggcatc cgccgcgatc
     3061 accttgctgc ccctgggtgc cgcgatgtcg agcgccctgt ggtacgagct gtagtactgg
     3121 gtgatcacgc cgccgggcac cggccacagc
//
LOCUS       DATCQR010000041         3933 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_17428, whole genome shotgun sequence.
ACCESSION   DATCQR010000041 DATCQR010000000
VERSION     DATCQR010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3933
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17428"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..520
                     /locus_tag="VIN32_01420"
     CDS             <1..520
                     /locus_tag="VIN32_01420"
                     /inference="COORDINATES: protein motif:HMM:NF015415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HEY8921264.1"
                     /translation="NAMNASPAGDMLPLLLACDAQFVAGAAHGERWIPVRDFWPSYRH
                     TALEPNELLLRISIPLPGDRQLRYRKVGTRRAQAISKVVMALAWLPGDDASWREVHLA
                     LGSVAPTAIRAPATEAVLEGSAPTEATADRAASTLAGELSPIDDVRSSADYRRAVAAR
                     VLHRLIRDEGGW"
     gene            520..2106
                     /gene="aceB"
                     /locus_tag="VIN32_01425"
     CDS             520..2106
                     /gene="aceB"
                     /locus_tag="VIN32_01425"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013704010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HEY8921265.1"
                     /translation="MADLPGGIRLTGPSVDHAEEVMTPEALAFVAHLHRSFDHRRREL
                     LAARTERQAAFDAGALPDFLPETAHIRDDSTWRVEAAPADLEDRRVEITGPVEPKMMI
                     NALNSGAKVFMADLEDALSPTWVNVVAGQWAIREAVRRRLTFQTDEKTYALQPRIATL
                     VIRPRGWHLEETHLQVDSSPVSASLFDFGLAFFHNASEQLERGSGPYFYLPKLESHLE
                     ARLWNDVFVEAQAKLGIPRGSIRATVLVETILAAFEMDEILWELREHASGLNAGRWDY
                     IFSLIKKFHSRTDMTLPDRAQVTMAVPFMRAYQQLLVRTCHARGAHAIGGMSAFIPNR
                     RLPEVTDNAVARVQEDKRREAGDGSDGTWVAHPDLVPVAAEVFDRVLGDRPNQKDRLR
                     PDVNVSAGELLDTRVEGGRVSEAGVSTNVSVALQYLASWLAGNGAAAIDNLMEDAATA
                     EISRSQLWQWRVHGTKLDDGRPMTRELYATIRDAELDRLAASAPDYRWTDAASLLDDL
                     VLGDGFPEFLTLAAYPMLER"
     gene            complement(2112..3503)
                     /gene="hydA"
                     /locus_tag="VIN32_01430"
     CDS             complement(2112..3503)
                     /gene="hydA"
                     /locus_tag="VIN32_01430"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HEY8921266.1"
                     /translation="MPDLVVRGGTIVTVARSFRGDVAISGGRISEVAEPLADAPPDTP
                     TLDATGLLVLPGCIDVHTHTRLPSDDEPDRFFADSVAAAFGGTTTFLAFNNPGTGISE
                     DGSRSLIAGMREFRARTDDESAIDYGLSAVISGQQDDPISELPELIASGVPTAKAFMV
                     FDFRLTDERLFGALRAMARHGGMLQVHCENATVIDALVADAIARGNVGCRHHALTRPA
                     YAEAEATHRAIALARAADAPVYIVHLSCADALAEVAAAKARSWPVYAETCPHYLSLTD
                     ARYDAPDEQDVIKSVISPPLRSESDREALWLGLRAGGLDLVASDHVPDRVAVEKLCPA
                     PPFPQISNGAPGIETLLAVVYDEGVGGGRISVERMVDMLATTPARLFGLTSKGAIEVG
                     RDADLVLFDPQAKRTIGQSELHHTSDFTPYEGREVHGAVRSVLLRGQPVIRDGAFVGG
                     RGAGRFMERSTAP"
     gene            complement(3600..>3933)
                     /locus_tag="VIN32_01435"
     CDS             complement(3600..>3933)
                     /locus_tag="VIN32_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014995664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3830 family protein"
                     /protein_id="HEY8921267.1"
                     /translation="VRRLLPMRSQLIHCRWSGEGTWIPLGDLQTGVGYERHTSHPSPG
                     ELLIYTGNLSECEILFPYGACMFSSKLGQLAGNHFAMVTSGAEQLPEMGRRVLWDGAQ
                     PIEIEEAE"
BASE COUNT          631 a         1358 c         1326 g          618 t
ORIGIN      
        1 caacgcgatg aacgcctcgc ccgcggggga catgctgccg ctcctgctcg cctgcgacgc
       61 ccaattcgtc gccggtgctg cgcacggcga gcgctggatc cccgtccgcg acttctggcc
      121 gagctaccgg cataccgcgc tggagccgaa tgagctgctg cttcggatca gcatcccgct
      181 gcccggcgac cgtcagctcc gctatcgcaa ggtcggcacg cggcgggcgc aggcgatcag
      241 caaggtggtg atggcgctcg cctggctccc gggtgacgac gcatcgtggc gcgaggttca
      301 cctggcgctc ggatcggttg cgccgactgc gatccgcgca cctgccacgg aagcagtgct
      361 cgagggttca gcaccgactg aggcaacggc ggaccgcgcc gcctcgacgc ttgccggcga
      421 gctcagtcca atcgacgacg tgcggtcaag cgctgattac cgccgcgcgg tcgccgcccg
      481 cgtgctgcat cggctgattc gggacgaggg cggctggtga tggccgattt gccagggggt
      541 atccgcctga cgggtccatc ggtcgaccat gcggaggagg tgatgacacc ggaggcgctc
      601 gccttcgtgg cgcacctcca tcggtccttc gaccatcggc gacgggagct cctggcggct
      661 cgcaccgaac ggcaggctgc attcgatgcc ggcgcacttc ccgacttcct gcccgagacc
      721 gcgcacatcc gcgacgactc cacctggcga gtcgaggctg cgccggccga cctggaggat
      781 cgacgcgtcg agatcacggg tccggtggag ccgaagatga tgatcaacgc gctcaactcc
      841 ggcgccaagg tcttcatggc cgatctcgag gacgccctgt cccccacctg ggtgaacgtc
      901 gtcgccggcc aatgggcgat tcgcgaggca gtccgccggc ggctgacatt ccagaccgat
      961 gagaagacct atgccctcca gccaaggatc gcgacgctgg tgatccggcc gcgcggctgg
     1021 cacctggagg agacccacct gcaggtcgac agctccccag tctcggccag cctgttcgac
     1081 tttggcctcg ccttcttcca caacgcaagc gagcagctcg agcgcggatc gggcccctac
     1141 ttctacctgc ccaagctcga gtcgcatctc gaggcgcgtc tctggaatga cgtcttcgtg
     1201 gaggcgcagg cgaagctcgg catcccccgc ggcagcatcc gcgccacggt cctggtcgag
     1261 acgatcctgg cggccttcga gatggacgag atcctgtggg agcttcgcga gcacgcatcg
     1321 ggcctgaacg cgggccgctg ggactacatc ttcagcctga tcaagaagtt ccactcccgc
     1381 accgacatga ccctgccgga tcgggcgcag gtcaccatgg cggtgccctt catgcgcgcc
     1441 taccagcagc tcctggtgcg cacctgccat gcgcgaggtg cccacgcgat cggtggcatg
     1501 agcgcgttca tcccgaatcg gcggctgccg gaggtgaccg acaacgccgt cgcccgggtg
     1561 caggaggaca agcggcgcga agcgggcgat ggctcggatg gcacctgggt tgcgcatccg
     1621 gacctggtgc ccgttgccgc cgaggtcttc gaccgggtgc tcggcgatcg accgaaccag
     1681 aaggatcggc tccgcccgga tgtcaatgtg tcggccggcg agctgctgga cacgagggtg
     1741 gaaggcggcc gcgtgagcga ggcgggagtc tcgaccaacg tcagcgtggc gctccagtac
     1801 ctcgcttcgt ggctggccgg taacggggcc gctgccatcg acaacctgat ggaggacgca
     1861 gcgactgccg agatcagccg cagccagctc tggcaatggc gggtgcacgg aacgaagctc
     1921 gacgacggca gaccgatgac gcgtgagctc tacgcgacga ttcgcgacgc cgagctcgat
     1981 cgcctcgctg catcggcgcc cgactatcgc tggaccgatg ccgcctccct gctggacgac
     2041 ctggtcctgg gcgatggctt ccccgaattt ctgacgctcg ccgcctaccc gatgctcgaa
     2101 cgctgacttc gctacggggc ggtcgagcgc tccatgaagc gccccgcccc gcgaccaccg
     2161 acgaatgctc cgtcccggat gaccggctgc cctcgcagga gcaccgagcg aaccgcgcca
     2221 tgcacctctc ggccctcgta tggggtgaag tcagaggtgt gatggagctc cgactgcccg
     2281 atggtgcgct tcgcctgcgg atcgaagagg acaaggtcgg cgtcccgccc gacctcgatc
     2341 gcgcccttgc tggtcaggcc gaagaggcgg gccggcgtgg tggccagcat gtcgaccatc
     2401 cgctccacgc tgatccgccc gccgcctacc ccttcgtcgt acaccacggc gaggagggtc
     2461 tcgatcccag gcgccccgtt gctgatctgc ggaaatggcg gagctgggca gagcttctcg
     2521 acggcgactc gatcaggcac gtggtcgctg gcgaccaggt ccagcccacc ggcgcgcagt
     2581 ccgagccaga gcgcctcgcg gtcggattcc gagcggaggg gtggcgagat gacgctcttg
     2641 atcacatctt gctcgtcggg ggcgtcgtag cgggcatcgg tcaggctcag gtagtgcggg
     2701 caggtctcgg cgtagaccgg ccagctccgt gccttcgccg cggcaacctc agccagcgca
     2761 tcggcgcacg agagatggac gatgtagacc ggtgcatccg cagccctggc cagcgcgatc
     2821 gctcggtggg tcgcctccgc ctcggcgtag gccggtcggg tcagcgcgtg gtgccggcag
     2881 ccgacgttcc cgcgggcgat ggcgtcggcg accagcgcat cgatgaccgt ggcgttctcg
     2941 cagtggacct ggagcatccc gccatggcgg gccatcgctc gcagcgctcc gaagaggcgc
     3001 tcatcggtca ggcggaagtc gaagaccatg aatgccttgg cggtcggaac ccccgaggcg
     3061 atcaactccg gcagctcgct gatcgggtcg tcctgctggc cgctgatcac cgccgagagg
     3121 ccgtagtcga tcgcggactc gtcgtcggtc ctggcgcgaa actcccgcat ccccgcaatc
     3181 agcgagcgtg acccatcctc gctgatgccc gtgccggggt tgttgaaggc aaggaaggtg
     3241 gtggtcccgc cgaaggcagc cgccacggaa tcggcgaaga agcggtcagg ctcgtcgtca
     3301 ctcggcagcc gggtgtgggt atggacgtcg atgcaacccg gcaggaccag cagcccggtg
     3361 gcgtcgaggg ttggcgtgtc gggaggtgca tcggccaatg gttccgccac ctccgagatg
     3421 cgcccacccg agatcgccac gtcaccccgg aacgagcgtg cgacggtgac gatcgttccg
     3481 cccctgacga cgaggtcagg caccctccgc gtccgttggg cgacgtcgcc gtctgcccgt
     3541 cgtcgccacg aggagcacca ccaatacgat gaccgcgagg accacccact ctcggcccat
     3601 cactcggcct cttcgatctc gatcggctgc gccccgtccc atagcacgcg gcgtcccatc
     3661 tcgggaagct gctccgcacc actggtcacc attgcgaagt gattcccggc gagctgccca
     3721 agcttcgacg agaacatgca cgccccatac gggaagagga tctcgcactc ggacaggttc
     3781 ccggtgtaga tcagcagctc gcccggggac ggatgcgagg tgtggcgctc gtagccgacg
     3841 cccgtctgca ggtcgcccag ggggatccat gtcccctcgc cgctccagcg gcaatggatc
     3901 aactgcgagc gcatcggtag caggcgtcgc acc
//
LOCUS       DATCQR010000042         9283 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_94839, whole genome shotgun sequence.
ACCESSION   DATCQR010000042 DATCQR010000000
VERSION     DATCQR010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 9283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9283
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94839"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..847)
                     /locus_tag="VIN32_01440"
     CDS             complement(<1..847)
                     /locus_tag="VIN32_01440"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY8921268.1"
                     /translation="MRCSVSGGGTGGHIYPALAVARALRDAQPELEVSYIGGARGLER
                     TLVANGELPYHQLVVRSLRSTGADLHLVLDPLRLAASVPQAWRLLGRLRPAAIFTTGG
                     YLALPLVSAARARGIPSLLWEGNVIPGRATATVARLATRVAVSFPPTLDAFPGRSFVS
                     GTPIRSFAGIDRAAARASFGVGSDERLLLVFGGSQAVPRITAALDAALERLLPDWRIL
                     HLAGPAGMPAALAMRERLRKTLAERYEPVQFLTDRMAEALVASDLVLGRAGSSTCAEV
                     AAVGVS"
     gene            complement(753..2012)
                     /gene="ftsW"
                     /locus_tag="VIN32_01445"
     CDS             complement(753..2012)
                     /gene="ftsW"
                     /locus_tag="VIN32_01445"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HEY8921269.1"
                     /translation="MTAIAATTTRRTARAGVRRERHEASFPLLISVIALVAIGVVMVY
                     SASSVRSYISTSDPGSQGFQQGIWAALGLVLMFGASRTDFRVLRYAAIPVFGLTMVLL
                     AIVLVPSIGTSAFGSRRWLFLPWIGGFQPAELAKLAVCLYLAHWLDRRGIAARGMANG
                     LLPFVILVAPGFLLIALEPDLGTAGVYAAASLAIFFMAGANLLYLGAIGAAVIGVAYH
                     MVTQTEYQLQRINSFLDPFKDPLHHGYNAVQSLMALALGGITGAGLGASRQKYLYLPA
                     PSTDFIFAIIGEEWGLIGTLTVLALFVMVAYQGYRIAIQAPDTFSGLLAAGVTTWLVA
                     QACINMMVVTALLPVTGIPLPFISYGGSALIINLAAVGILLSISRETTQTGSVIDAVF
                     GVGRRDRRAHLPRAGRRKGTARRAARA"
     gene            complement(2009..3436)
                     /gene="murD"
                     /locus_tag="VIN32_01450"
     CDS             complement(2009..3436)
                     /gene="murD"
                     /locus_tag="VIN32_01450"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEY8921270.1"
                     /translation="MSTMTAPITTLEDLRGRHAVVLGLARSGTAVARLLADAGADVAA
                     YDRRPATELAEAVQALGGRRVRLALGVSADEARTLIETADLLVTSPSISPSMPTTDAW
                     LRAAIREAVARGVELVSEVELFLRLTQARVLAVTGTKGKTTTTSLIGAIMDAAGMPHL
                     LGGNIGRPLIEEVERLGPEDWAVVELSELQLPTISRGAEIAVYTNIGADHLDRHGTEA
                     AYRAVKARLAQLSVTHGHVVLNADDPGCRELGARLPAASIAWYALVADDSWMAARLID
                     GWLTIRGERVLAAAEVPVPGRHTLADALAAALATSLAGASNEAIAAGIRDFGGVPHRL
                     ERVATRQGVAWVNDSQATIPMAAIAALDAFAPAEVVLIAGGKDKGLDYAAFADAITGR
                     CRAVILIGETADRLEELIEGRVPVVRAEEMRDAVAAAAAWAVAGDVVVLAPAAASFDM
                     FDDYAARGDAFRAAVRALDAEEPDA"
     gene            complement(3442..4452)
                     /gene="mraY"
                     /locus_tag="VIN32_01455"
     CDS             complement(3442..4452)
                     /gene="mraY"
                     /locus_tag="VIN32_01455"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEY8921271.1"
                     /translation="MTDMLPIVVALLAAFAGVVLLGPVYIGILQRLGFGKQIRSDGPE
                     GHFGKAGTPTMGGMLLVVVVMFLAMVMRIEDGYTLTPMLALVGVAILGAIDDFVNVHT
                     GIGVRGRFKLIWQTVVALLAAFYIQRHFAIPGVNISFISVPFPFAGEVVIAGVLFVLF
                     VAFVIVGVSNAVNLTDGLDGLAGGVLIFSFVAYLLIALVAAGGKPSQPNLAIFCALII
                     GALMGFLWFNVHPAQLFMGDSGALGMGATLAVVATITAQLPLLAVIGAVFFAVIMSVV
                     LQVASFRLRGRRIFRMAPLQHHFELIGWAEEKITVRFWIVGALAGLLGFSVYLATVPG
                     LK"
     gene            complement(4445..5851)
                     /gene="murF"
                     /locus_tag="VIN32_01460"
     CDS             complement(4445..5851)
                     /gene="murF"
                     /locus_tag="VIN32_01460"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEY8921272.1"
                     /translation="MPPSIRLDDLLAATGARLLGPTSVTSFATAAVDSRNVVPGCLFV
                     ALRGERTDGHRFAADAVAAGATVLLVEHLVELPADAQVAVLQVADPLNALQEMAAWWR
                     ARSSVRVVGITGSTGKTIAKEIVADVLSRTLRVLRNDGNLNSETGLPMTLLRLEPWHE
                     ASVLEMSMYTEGEIARLVEIARPEVGVVLAVHPTHLERAGSLDAIARAKSELPAALPP
                     DGLAVLNADDPRVAAMRSVTAATVRTFGLGPGADVRATEIESQGVEGTTFMLEAPWGR
                     RRLHSAIPGRHLVPHALAAAAVADHFGVPLDDVEAALAAGSRADHRMAVVEATAGATV
                     IDDTYNASPVSVGAALDFLGESPLPPGRARYAVLGDMLELGPDEERLHREVGAKAAGV
                     ADALVAVGERGRWIAEGARAAGLRRVTTVQDAEEAALAVERDLAPGVGDLLLVKGSRG
                     IELDRLVAALSGQAPGDD"
     gene            complement(5890..7713)
                     /locus_tag="VIN32_01465"
     CDS             complement(5890..7713)
                     /locus_tag="VIN32_01465"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEY8921273.1"
                     /translation="MLARTDSRARALFLLIITAMLATVVGGRLVFWQVVDRERVAALA
                     LNQLAQNQEIPAERGEIRDANGVLLATSVEVQSVYAIPPTVQQGDPQRAATQLASVLG
                     MKVDDVLARLTGKDPWVWLKRRIDPKMAQWVAALDIPGVGMIPETQRVYPISGVAPQT
                     TLGAQLLGFVNVDGQGQYGVEGAEDALLAGLPGSVSAQEDVIGRRIADSVYELRPPVN
                     GADLHLTLDAGLQHILEAAMWSTYQKNLAQGVTGLVMDVHTGAIKAMASFPTFDANSY
                     KSTPPALFTDPAVSRQYEPGSVMKAFTVAAALDAGAIDLQTTVKDDNNLRIGGIRIQN
                     ADRYTSPYGHGNITAGDVLKLSNNIGAAKIGLLLGRQRLYEAFKRFGFGTPTGVDIAG
                     EASGVVWNPDGPNASGNLTAAQNAFGQGLSVTALQLVAGYCAIANGGTLVTPHVIAGW
                     TGPDGVYHATDVQQGERIMREETAHTTLQLLLDAVDYGIAQKASIPGYSIAGKTGTAE
                     VAGPKTERVKVGTDDAGNPIYAMKTRLVYPHGWIDSSFISVMPASDPQFVTLILIHRP
                     VTWGLYKMAQTPVSVFRTLAPQMLDYLAIAPDRVVAPVAAK"
     gene            complement(7748..8164)
                     /locus_tag="VIN32_01470"
     CDS             complement(7748..8164)
                     /locus_tag="VIN32_01470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921274.1"
                     /translation="MAIVGARPATGIGLVRRRPRARTRPAAPARRRVHRVARSRGTIG
                     GFSTVLVAIAAAAGLCLFYLSQSTHVAALGYQIDGLEARVADLRAEQQQLTFEIGVAR
                     SPSTIEVEAQNELRLVPLDPTVVRFATRSIDQTLLK"
     gene            complement(8165..8374)
                     /locus_tag="VIN32_01475"
     CDS             complement(8165..8374)
                     /locus_tag="VIN32_01475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921275.1"
                     /translation="MSRGRPRHQSSRRRMYSGRQRDLRERRTHQEWDAPGAFEQDGAP
                     EADDTRELESPTGWSIQLNARPSAA"
     gene            complement(8395..>9283)
                     /gene="rsmH"
                     /locus_tag="VIN32_01480"
     CDS             complement(8395..>9283)
                     /gene="rsmH"
                     /locus_tag="VIN32_01480"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:NF013919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEY8921276.1"
                     /translation="SSIADCTVGGGGHAERLLEASSPDGRLLGLDADRAAILETQRTL
                     ARFGDRVILRQANFANLYDEARATDLIPLDAVLFDLGLSSYQLADPQRGFSFAADAPP
                     DMRFDEDRGLSALELIDGSSQRELAGIIATFGEERRAGRIATAILAARAQGRLMTAAD
                     LAEVVAAAVPASRADAGRIHPATRTFQALRIAVNRELEVLTPALAGALAALQPGGRLA
                     VITYHSGEDRIVKRFMARESRDCVEDPLPPVCLCGHRAQLRPIVRRGITPTAAEVSRN
                     RRARSARLRAAIKLAPAGA"
BASE COUNT         1485 a         3191 c         3295 g         1312 t
ORIGIN      
        1 aggagactcc gaccgcggcc acctcggcgc aggtcgacga gccggcgcgc cccagcacga
       61 gatcgctggc aaccagtgcc tcggccattc gatcggtgag gaactggacc ggctcgtacc
      121 gttcggcgag ggtcttgcgg agccgttcgc gcatggcgag cgcggcgggc atccccgccg
      181 ggccggccag gtgaaggatc cgccagtcag gcagcagccg ctcgagcgcc gcatcgaggg
      241 cggcggtaat gcgcgggacc gcctgcgacc cgccgaagac cagcagcagg cgctcgtccg
      301 acccaacgcc gaatgacgcg cgagccgccg cgcggtcgat cccggcgaag gagcggatgg
      361 gagtgcccga gacgaagctg cgtccgggaa acgcatcgag cgtcggcgga aaggagaccg
      421 ccacccgcgt ggcgaggcga gcgaccgtcg ccgtcgcccg acccgggatc acgtttccct
      481 cccacaggag gctcggaatg ccgcgggcgc gggcggcgga gaccagggga agggccaggt
      541 agccgccggt ggtgaagatg gccgccgggc gcagccggcc gaggagccgc catgcctgtg
      601 gcacggacgc tgccaggcgg agcgggtcga gcacgaggtg caggtcggcg ccggtgctgc
      661 gcagcgagcg caccaccagc tggtggtagg gcagctcgcc attggcgacg agggtgcgct
      721 cgagaccccg cgcgccgccg atgtagctga cctcaagctc gggctgcgcg tcgcgcagtg
      781 cccttgcgac ggccagcgcg gggtagatgt gcccgcctgt cccgccgccc gacaccgaac
      841 accgcatcaa tcactgatcc tgtctgcgtg gtctcgcggg agatggacag cagaatgccg
      901 accgccgcga ggttgatgat gagggccgat ccaccgtagc tgatgaacgg gagcgggatg
      961 ccggtgaccg gcagcagcgc ggtgacgacc atcatgttga tgcaggcctg ggcgaccagc
     1021 caggtggtga ccccggcggc cagcaggccg gagaacgtat cgggcgcctg gatcgcgatc
     1081 cggtagccct ggtaggccac catcacgaac agggcgagca cagtgagggt cccgatcagc
     1141 ccccactcct cgccgatgat cgcaaagatg aagtcggtcg acggcgcagg caggtacaga
     1201 tacttctgcc tgctggcgcc gagcccggcg ccggtgatgc cgccaagggc cagcgccatc
     1261 agcgactgga cggcgttgta tccgtggtga agcgggtcct tgaaagggtc gaggaagctg
     1321 ttgatccgct gcagctggta ttccgtctgc gtgaccatgt gatacgcgac gccgatgacc
     1381 gccgctccga tcgctccgag gtacagcagg ttggcgccgg ccatgaagaa gatggccagc
     1441 gacgccgcgg cgtagactcc ggccgtcccg aggtccggct cgagcgcgat cagcaggaag
     1501 cccggtgcca cgaggatcac gaacggcagc aggccgttgg ccatgccccg agccgcgatt
     1561 cctcgccgat cgagccagtg ggccaggtac aggcagaccg ccagcttggc gagctcggcg
     1621 ggctggaagc caccgatcca gggcaggaac agccagcgcc gcgagccgaa cgccgaggtg
     1681 ccgatgctgg gaaccagcac gatggcgagc agaaccatgg tcagcccgaa gaccgggatc
     1741 gccgcatagc gcagcacccg gaagtcggtc cggctggcgc cgaacatgag caccagcccc
     1801 agcgccgccc agatcccctg ctggaacccc tggctgccgg gatccgaggt ggagatgtac
     1861 gagcgcacgc tcgaggccga atagaccatc accacgccga tggcgacgag cgcgatgacg
     1921 gagatgagca gcgggaacga ggcctcgtgg cgctcgcgcc gcacaccggc tcgggccgtg
     1981 cgccgagtcg tggttgcggc aatggccgtc atgcgtccgg ctcctcggca tccagggcgc
     2041 ggacggcggc gcggaaggca tcgccacgcg ccgcgtagtc gtcgaacatg tcgaacgagg
     2101 cggccgccgg ggccagcacg accacgtcgc cagcgacggc ccacgcggcc gcggccgcga
     2161 ctgcatcgcg catctcctcg gcccggacca ccggcacgcg gccctcgatc aactcctcca
     2221 gtcgatcggc cgtctccccg atcaggatca ccgcgcggca gcgacctgtg atcgcatcgg
     2281 caaaggccgc gtagtcgagc cccttgtcct tgcctcctgc gatcaggacc acctcggccg
     2341 gggcgaaggc gtccagcgcc gcgatcgcgg ccatcgggat cgtcgcctgc gagtcattga
     2401 cccacgccac gccctggcgc gtcgccaccc gctccagtcg atgcgggacg ccaccgaagt
     2461 cgcggatgcc ggccgcaatc gcctcgttgg aggctccggc cagcgaggtg gcgagggcgg
     2521 cggccagcgc atcggccagc gtgtgccgtc cggggacggg cacctcggcg gccgccagca
     2581 ctcgctcgcc gcggatcgtg agccagccat cgatcagccg ggctgccatc catgagtcgt
     2641 cggccacgag cgcgtaccag gcgatcgagg cagcgggcag gcgcgcgccg agctcccggc
     2701 agcccgggtc gtccgcgttc agcacgacat gcccatgcgt cacgctcagc tgcgcgagcc
     2761 gagccttgac cgcccggtag gccgcctcgg tcccgtgccg gtcgaggtga tcggcgccga
     2821 tgttggtgta taccgcgatc tccgcaccgc gtgagatggt cgggagctgc agctcggaga
     2881 gctcgaccac cgcccagtcc tcggggccca ggcgctcgac ctcctcgatc agcgggcggc
     2941 caatgttgcc gccgagcagg tgcggcatgc cggctgcgtc catgatcgcc ccgatcagcg
     3001 aggtggtcgt ggtcttgccc ttggtgccgg tcacggccag cacccgggcc tgggtcaggc
     3061 gcaggaagag ctcgacctcg ctgacaagct cgacgccgcg cgcgaccgcc tccctgatcg
     3121 cggctcgcag ccaggcgtcg gtggtcggca tcgagggcga gatgctcggg ctggtgacga
     3181 gcaggtcggc cgtctcgatc agggtccgcg cctcgtcggc gctgacgccc aacgccaacc
     3241 gcacccgccg gccaccgagt gcctgcacgg cctccgccag ctcggtcgcc ggtcgccggt
     3301 cgtaggccgc cacatcggcg ccggcatcgg ccagcaatcg cgccaccgca gtgccggagc
     3361 gtgcaaggcc gaggacgacg gcgtggcgtc cccgcaggtc ctcgagggtc gtgatcggcg
     3421 cggtcatggt gctcatcggt ctcacttcag ccctggcacg gtggcgaggt agacgctgaa
     3481 gccgagcagc ccggcaaggg cgccaacgat ccagaagcgg accgtgatct tctcctccgc
     3541 ccacccgatc agctcgaagt ggtgttgcag cggggccatg cggaagatcc gccggccgcg
     3601 tagccggaac gaggcgacct gcagcacgac gctcatgatc accgcgaaga agacagcacc
     3661 gatcacggcc aggaggggca gctgggcggt gatggtggcc accacggcca gggtggcgcc
     3721 catcccgagc gcgccagagt cgcccatgaa cagctgcgcc ggatgcacgt tgaaccacag
     3781 gaatcccatc agcgcaccga tgatgagggc gcagaagatg gcgaggttcg gctgggacgg
     3841 cttcccgcca gccgccacca gggcgatcaa caggtacgcc acgaaactga agatgaggac
     3901 cccgccggcg agcccgtcga gcccatcggt caggttgacg gcgttgctga cgcccacgat
     3961 cacgaaggcg acgaagagga cgaagaggac cccggcgatg acgacctcac cggcgaaggg
     4021 gaacgggacc gagatgaacg agatgttcac cccggggata gcgaagtggc gctggatgta
     4081 gaaggccgcc agaagcgcca ccaccgtttg ccatatcagc ttgaagcggc cgcgcacccc
     4141 gatccccgtg tgcacgttca cgaagtcgtc gatcgcgccg agtattgcga cgccgaccag
     4201 tgccagcatc ggggtcaggg tgtagccatc ctcgatgcgc atgaccatgg ccaggaacat
     4261 caccaccacg accagcagca tgccgcccat ggtgggcgtg cccgccttcc caaagtggcc
     4321 ctcgggtccg tcggaccgaa tctgcttgcc gaaccccagg cgctgcagga tgccgatgta
     4381 gaccgggcca aggagcacca cgccggcaaa tgccgcgagc agggcgacga cgatcggcag
     4441 catgtcagtc atcccccggc gcctggccgg agagcgccgc caccagccga tcgagctcga
     4501 tcccgcgcga ccccttgacc agcaggaggt cgccgacgcc gggcgcaagg tcccgctcga
     4561 ccgccagcgc cgcctcctcc gcgtcctgaa ccgtcgtcac gcgacgcagc cccgccgcgc
     4621 gtgccccctc ggcgatccag cggccgcgct caccgactgc cacgagcgcg tcggcgacgc
     4681 cggccgcctt cgcgcccacc tcgcggtgca gccgctcctc gtcgggaccg agctcgagca
     4741 tgtcgccgag gaccgcgtac cgcgcgcgac cgggaggcag cgggctctcg ccgagaaagt
     4801 cgagcgccgc gccgaccgac accggagagg cgttgtaggt gtcgtcgatg acggtcgccc
     4861 cggccgttgc ctcgaccacc gccatgcggt ggtcggcacg gctgcccgcc gcgagcgctg
     4921 cctcaacgtc gtcgagggga acgccgaagt ggtcggccac cgcggccgcg gccagcgcat
     4981 gcggcacgag gtgtcgcccg gggatcgccg agtggagccg ccgtcgtccc cagggcgcct
     5041 cgagcatgaa ggtggtgccc tccaccccct gcgattcgat ctcggtcgcg cgcacgtcgg
     5101 cgcccggtcc gaggccgaag gtgcggaccg tcgccgcggt cacgctccgc atggccgcga
     5161 ccctgggatc gtcggcattc aggaccgcga gcccgtcggg ggggagggca gccgggagct
     5221 ccgacttggc gcgggcgatg gcgtcgaggc tgccagcacg ctccaggtgg gtcgggtgga
     5281 ccgccagcac cacgccaacc tcgggccgtg cgatctcgac cagccgcgcg atctcgccct
     5341 ccgtgtacat gctcatctcg agcacgctcg cctcgtgcca gggctccagc ctgagcagcg
     5401 tcatcggcag gccggtctcg ctgttcaggt tgccgtcgtt gcgcagcacg cgaagcgtgc
     5461 gcgacaggac gtcggccacg atctccttgg cgatcgtctt gccggtcgag ccggtgatcc
     5521 cgaccacccg aacgcttgag cgggcgcgcc accaggcagc catctcctgg agggcgttga
     5581 gcggatcggc gacctgcagg acagcgacct gcgcgtccgc gggaagctcg accagatgct
     5641 cgacgaggag gacggtcgcg ccagcggcga cggcatctgc tgcgaagcgg tggccatccg
     5701 tgcgctcgcc gcgaagcgcg acaaagagac agcccggaac gacattgcgc gagtcaaccg
     5761 cggcggtggc gaaggaggtg acgctcgtcg gcccgaggag gcgggccccg gtggcagcca
     5821 ggagatcgtc gagccggatt gacggtggca cgctgctccc tgcagagggg tcggccgagt
     5881 ctagcagcct catttggcgg cgaccggagc caccacccga tcgggagcga tggccaggta
     5941 gtcgagcatc tgcggcgcca gggtgcggaa gacgctcacc ggcgtctggg ccatcttgta
     6001 caggccccag gtcacggggc ggtgaatcag gatcagggtc acgaactgcg gatcgctggc
     6061 cggcatgacc gagatgaagg aggagtcgat ccatccgtgc gggtagacga gcctggtctt
     6121 catcgcgtag atgggatttc cggcgtcatc ggtcccgacc ttgacccgct ccgtcttggg
     6181 tccggcgacc tcggccgtac ccgtcttgcc ggcaatcgag tagcccggga tggaggcctt
     6241 ctgggctatg ccgtagtcga ccgcatccag cagcagctgg agggtcgtgt gggcggtctc
     6301 ctcgcgcatg atccgctcgc cctgctggac gtcggtggcg tggtagacgc cgtctggtcc
     6361 ggtccagccg gcgatgacgt gcggcgtgac cagcgttccg ccgttcgcga ttgcgcagta
     6421 gccggccacc agctgcaggg cggtcacgct gaggccctgg ccgaaggcgt tctgggcggc
     6481 cgtcaggttg ccgctggcgt tggggccatc cgggttccac accacgcccg aggcctcgcc
     6541 ggcgatgtcg acgccggtcg gcgtgccgaa gccgaagcgc ttgaacgcct cgtacaggcg
     6601 ctgcctgccg agcagcagac cgatcttggc agcgccgatg ttgttcgaca gcttcagcac
     6661 gtccccggcg gtgatgttcc cgtgcccgta aggagacgtg tagcgatccg cgttctggat
     6721 gcggatccca ccgatgcgca ggttgttgtc gtccttgacg gtcgtctgga ggtcgatggc
     6781 cccggcatcg agcgccgcgg caacggtgaa cgccttcatc accgaccccg gctcgtactg
     6841 tcggctgacc gccggatcgg tgaagagggc cggcggggtg ctcttgtagc tgttcgcgtc
     6901 aaacgtcggg aacgacgcca tcgccttgat cgcaccggtg tgcacgtcca tcaccagccc
     6961 ggtcacaccc tgggcaaggt tcttctgata ggtgctccac atggccgctt cgaggatgtg
     7021 ctgcagtcca gcgtccagcg tcaggtgcag gtccgcgcca ttgaccggtg gtcgcagctc
     7081 gtagacggag tcggcgatcc ttcgcccgat gacgtcctcc tgggcgctca ccgacccggg
     7141 cagcccggcc agcagcgcgt cctccgcccc ctcgactccg tactggccct ggccatcgac
     7201 gttcacgaag ccgagcagct gcgcgcccag cgtcgtctgc ggtgccacgc cactgatcgg
     7261 gtagacccgc tgtgtctcgg gaatcatccc gaccccggga atgtcgaggg ccgcaaccca
     7321 ttgagccatc tttgggtcga tccttcgctt cagccagacc cacggatcct ttccggtcag
     7381 ccgggccagc acgtcgtcca ctttcatccc gagcaccgat gcgagctggg tggccgcccg
     7441 ctgcggatcg ccctgctgca cggtcggggg gatggcatac accgactgga cttcgaccga
     7501 tgtggccagc agcacgccat tggcgtcgcg gatctcgccg cgctcggcgg ggatctcctg
     7561 attctgggcg agctggttca gagcgagtgc ggccacccgc tcgcggtcca cgacctgcca
     7621 gaagacgagc cgcccgccaa cgacggtggc gagcatggcc gtgatgatca gcaggaacag
     7681 ggcgcgggca cggctgtcag tcctggcgag cacggctact ccgtcgaccg agggtctccc
     7741 ccggccgcta cttcaagagg gtttggtcaa tcgatctcgt ggcaaagcgg acgacggttg
     7801 gatcgagcgg caccaaccgc agctcgttct gcgcctccac ctcgatggtg ctcggcgagc
     7861 gggccacgcc gatctcgaag gtgagctgct gctgttcggc gcgcaggtcg gcgactcgtg
     7921 cctccaggcc gtcaatctgg tatccgagcg cggcgacatg cgtcgactgg ctcaggtaga
     7981 agaggcacag gccggcggct gcggcgatcg cgaccaggac ggtcgagaag ccgccgatcg
     8041 ttccgcggct gcgggcgacg cgatggaccc gccggcgtgc cggggcggcg gggcgcgtgc
     8101 gagcacgagg ccggcgacgg acgaggccga tcccggtcgc tggacgcgcc ccgacgatgg
     8161 ccatctaggc ggcgctgggt cgcgcgttga gctggatgct ccagccggtc gggctctcga
     8221 gctcgcgagt gtcatccgcc tcgggtgcgc catcctgctc gaaggcgcct ggtgcatccc
     8281 actcctggtg cgtgcgccgc tcgcgcaggt cgcgctggcg gccgctgtac atgcgacggc
     8341 gactggactg atgacggggg cgtcctcggc tcatgggtac cctcctcgag ctggttatgc
     8401 gccggccggc gcgagcttga tggccgctcg caggcgtgcg ctgcgagctc ggcggttgcg
     8461 actcacctcg gcggcggtcg gcgtgatgcc cctcctgacg atgggacgga gctgtgcgcg
     8521 atggccgcac aggcagacgg gtgggagcgg atcctcaacg cagtcgcggc tctcccgtgc
     8581 catgaagcgc ttgacgatcc gatcctctcc ggagtggtac gtgatgacgg ccagccggcc
     8641 gccgggttgg agcgcggcca gggcaccggc gagtgccgga gtcagcacct ccagctcgcg
     8701 gttgacggcg atgcggagcg cctggaaggt gcgcgtggcg gggtgaatcc gtcccgcgtc
     8761 ggcgcggctt gcggggacgg ccgcggcaac gacctcggcc agatcggctg cggtcatgag
     8821 ccgcccttgc gcacgcgccg ccaggatcgc cgtcgcgatg cggcctgcac ggcgttcttc
     8881 accaaacgtc gcgatgattc cggcgagctc tcgctgcgac gatccgtcga tgagctcgag
     8941 ggccgataga ccacgatctt catcgaatct catgtccggc ggcgcatcgg ccgcaaagct
     9001 gaatccgcgt tgaggatcgg ccagctggta gctcgagagg ccgaggtcga agaggacggc
     9061 atcgagcgga atgagatcgg tcgcgcgcgc ctcgtcgtag aggttcgcga agttcgcctg
     9121 tcggaggatg acgcgatcgc caaagcgagc gagcgtccgt tgcgtctcca ggatggccgc
     9181 ccgatcggcg tcgaggccga gcaggcggcc atccggggac gaggcctcca agagccgctc
     9241 ggcgtgccca ccgccgccga ccgtgcagtc tgcgatggag ctg
//
LOCUS       DATCQR010000043         2389 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_33159, whole genome shotgun sequence.
ACCESSION   DATCQR010000043 DATCQR010000000
VERSION     DATCQR010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2389
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33159"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..545
                     /locus_tag="VIN32_01485"
     CDS             <1..545
                     /locus_tag="VIN32_01485"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8921277.1"
                     /translation="DARGDAARLADLAADLIELVAAEATGRPLVRTPTSVSDLADSVV
                     RRLETLARAAEVSVAIDATDAQVSLDRVRADQAVGNLVANAIAHAPPGSTVEVRARVE
                     SAEAGPMLEVDVLDRGPGVRLEERDLIFEPFQRGVGARGGGAGLGLATARAAAEAHGG
                     EIGVDDRPGGGARFWIRLPV"
     gene            complement(552..1781)
                     /locus_tag="VIN32_01490"
     CDS             complement(552..1781)
                     /locus_tag="VIN32_01490"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HEY8921278.1"
                     /translation="MRRVAVTGIGAVTPIGSGADGLWAGVLANRSAVQAIDRFDASPF
                     PSRIAAQINDFVPADHLDAKRARRLDRFSQLSVAAARMAVVQSCLTNADRSDGRTGVW
                     IGSALGGVAFGEEQHAAYVLGGVRAVAPTLATAVFGGAGASNVAIDLGSRGPAVGNAN
                     SCASGAVAIGQAFGAIRSGIVDVAIAGGAEAPLAPLTHGAFAMIRVLSTRNDDPASAS
                     RPFDRDRDGFVMAEGAAMLVMEAWEHAERRGATILGEVAGFGASTDAYHLTAPLPSGE
                     AAAAAITTALADAGVAAGEIGYVSAHASSTQLNELAETRALQLALGEHAAEVPVSGTK
                     GLYGHALGASGAIEAAITVMALRNGLLPGTCNLVNLDGRCDLNVLREPLAARPRAAIS
                     TSFGFGGMNAALVFRTV"
     gene            complement(1778..2209)
                     /locus_tag="VIN32_01495"
     CDS             complement(1778..2209)
                     /locus_tag="VIN32_01495"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY8921279.1"
                     /translation="MRIHIDAPLERIFPLAAEVERWPERLPHYRYVHRLPASNGERRF
                     AMGARRGPIPVRWEAIQRPLPEERRIEFTHTAGVTRGMQVAWRFEPSDSGWEVSIEHE
                     LELRWPFIGALAAEQVIGPQFVEAIARRTLRRVKVLAEAGS"
BASE COUNT          357 a          864 c          853 g          315 t
ORIGIN      
        1 gggacgctcg aggagacgct gcacggcttg ccgacctggc cgccgacctc atcgagctcg
       61 tcgcggccga ggcgactggc cgccccctgg tgcggacccc gacgagcgtc agcgacctgg
      121 ccgacagtgt ggtgcggcgc ctggagacgc tggcacgggc agccgaagtc tccgtggcga
      181 tagacgccac cgatgcgcag gtgagcctcg accgggtacg ggccgatcag gcagtgggca
      241 acctcgtggc gaatgccatc gcccatgcgc ccccgggttc aaccgtggaa gtccgcgcaa
      301 gggtggagtc cgcggaagcg ggcccgatgc tcgaggtcga cgtgctggac cgcgggccgg
      361 gcgtccgtct ggaggaacgg gatctcatct tcgagccctt ccagcgcgga gtcggcgctc
      421 gaggcggcgg tgctggactg ggtcttgcaa cggcgcgggc ggcggccgag gcgcacggtg
      481 gcgagatcgg ggtggatgat cgacccggag gtggcgctcg gttctggata cgccttcccg
      541 tctgacgcct gctacaccgt gcgaaagacg agcgccgcgt tcatgccacc gaagccaaac
      601 gaggtcgaga tcgcggcccg gggccgcgcc gcgagtggct cgcgcaggac gttcaggtcg
      661 cagcgcccgt cgaggttgac caggttgcag gtccccggca ggagaccgtt gcgcagcgcc
      721 atgaccgtga tcgccgcctc gatcgcaccg ctggctccca atgcgtggcc atacaggccc
      781 tttgtgcccg agaccggcac ttccgccgcg tgctccccca gtgccagctg aagcgccctc
      841 gtctcggcca gctcgttgag ctgcgtgctg gaggcgtgcg cactgacgta gccgatctcc
      901 cctgccgcga cgcccgcgtc cgcgagggcg gtcgtgatcg ccgcggctgc agcctcgccg
      961 gaggggagcg gcgccgtcag gtggtaggca tcggtcgaag cgccgaagcc ggccacctcg
     1021 cccaggatgg tggctccgcg ccgctccgca tgctcccacg cctccattac cagcatcgcc
     1081 gctccctccg ccatcacgaa cccatcgcgg tcgcgatcga agggacgcga ggcgctggcc
     1141 gggtcgtcgt tgcgcgtcga caggacgcgg atcatcgcga acgcgccgtg ggtcagcggc
     1201 gccagcggcg cctccgcgcc accggcgatc gcgacgtcga cgatccccga cctgatcgca
     1261 ccgaacgcct gcccgatggc gaccgcgcca gaggcgcagg aattggcatt ccccaccgcc
     1321 ggcccgcgac tgccgaggtc gatcgccacg ttgctcgccc cggctccgcc aaagacggcc
     1381 gttgcaagcg tgggcgccac cgcccgaact ccgccgagca cataggccgc gtgctgctcc
     1441 tcgccgaagg cgaccccgcc cagggccgac ccgatccaga ccccggtgcg gccatcggac
     1501 cgatcggcat tggtcaggca ggactggacc acggccattc gcgcggcggc gaccgagagc
     1561 tggctgaacc gatccaaccg ccttgcgcgc ttcgcgtcga ggtggtcggc tgggacgaag
     1621 tcgttgatct gcgcagcgat ccgcgatggg aatggcgagg cgtcgaagcg atcgatcgcc
     1681 tgaaccgccg agcggttggc cagcacgcca gcccacagcc cgtcagctcc cgagccgatg
     1741 ggagtcacgg ccccgatccc ggtcaccgcc acccgtctca cgagcctgcc tccgccagga
     1801 ccttcacgcg gcggagcgtg cgacgcgcga tcgcctcgac gaactgcggc ccgatgacct
     1861 gctcggccgc cagcgcgccg atgaacggcc agcgcagctc cagctcgtgc tcgatgctga
     1921 cctcccagcc ggagtcgctc ggttcgaagc gccaggccac ctgcatgccg cgcgtcacac
     1981 cggcggtgtg cgtgaactcg atccgccgct cctcggggag cgggcgctgg atcgcctccc
     2041 agcgcacggg gatcgggccg cggcgagcgc ccatggcgaa gcgtcgctcc ccgttcgagg
     2101 cgggcaggcg atggacgtag cggtagtgag ggaggcgctc cggccagcgc tcgacctcgg
     2161 ccgccagcgg gaagatccgc tccagcggag cgtcgatgtg gatccgcacg aggctcctca
     2221 cggtcgaagg acctgcgcca ggaagagcgg cgagagcgcg tcgctggccg ggcggcaatc
     2281 gcccagggcg ctgcccagcc gctcggccgt ggcgggccgg gatgccagtc gccgcagcgc
     2341 gtaccccatg agcggagggg aggcgagcat cccctggacg agccaggta
//
LOCUS       DATCQR010000044         1934 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_110640, whole genome shotgun sequence.
ACCESSION   DATCQR010000044 DATCQR010000000
VERSION     DATCQR010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1934)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1934)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1934
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110640"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1564
                     /gene="nuoL"
                     /locus_tag="VIN32_01500"
     CDS             <1..1564
                     /gene="nuoL"
                     /locus_tag="VIN32_01500"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005141.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEY8921280.1"
                     /translation="LLILGDNFLMLYVGWEAVGLCSYALIGFWYKKPSAAGAAKKAFI
                     VNRVGDFGFGLGIMMIWTLLGTLDFGQVFSSIGTLDPTAITVISLLLFAGAVGKSAQF
                     PLHVWLPDAMEGPTPVSALIHAATMVNAGVYMVARANPIFSHSPTAMWVVASIGVFTA
                     IFAAAIALTQNDIKKVLAYSTVSQLAYMFFALGIGAWVAAIFHLVSHGFFKGLLFMGS
                     GSVIHGAAGEQDMRFMGGLRERMPWTYRTMVVGSLALAGIPIFAGFFSKDEILGEAFG
                     RGYYVFWAVGLVVAFMTAFYTFRMVYMTFHGRWRGPADAWQHVHESAPTMVAPLVILA
                     VPTILVGLLLGLPPEGGLIHGWLGDVFRGAEEAGAGILPGSIASGAHAGFSFFGIGGL
                     LLLLGAGVGAAGIWLAHRWYVLDPEAPARFVARIPFGLGPGMYAASVNKYYVDDLYLL
                     VWARGGVLVGNALWWFDAKVIDGAVNGAGWIAQRLGGLLRRSQTGHVQNYGLGMAAGL
                     VVVLLAFLVIRG"
     gene            1561..>1934
                     /locus_tag="VIN32_01505"
     CDS             1561..>1934
                     /locus_tag="VIN32_01505"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Catalyzes the transfer of electrons from NADH to
                     quinone; Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEY8921281.1"
                     /translation="MSGLPTLSLVVFTPLIGVLLILFVPKDNHRLIRWIALLAALASL
                     GFSLFLLGYRPDGAEFQFREDISWISAFGMRYTIGVDGLSVVLVMLTTVLSVVSIFYS
                     WDPIQTRVKEYYATMLLLMIGML"
BASE COUNT          250 a          653 c          656 g          375 t
ORIGIN      
        1 gctgctgatc ctgggcgaca acttcctgat gctctacgtc ggctgggagg cggtcggcct
       61 gtgcagctac gcgctgatcg gcttctggta caagaagcca tcggcggcgg gtgccgccaa
      121 gaaggcgttc atcgtcaacc gggtcggtga cttcggcttc gggctcggga tcatgatgat
      181 ctggaccctg ctcggcacgc tcgacttcgg gcaggtcttc agcagcatcg gcaccctcga
      241 cccgacggcg atcacggtca tctcgctcct cctcttcgcc ggtgcggtgg gcaagagcgc
      301 gcagttccca ctccacgtct ggctgcctga cgcgatggag gggccgacac cggtcagcgc
      361 gctgatccat gccgcgacga tggtcaacgc cggggtctac atggtcgccc gcgccaaccc
      421 gatcttctcg cacagcccca cggcgatgtg ggtggtggcc agcatcggcg tcttcaccgc
      481 gatcttcgcc gccgcgatcg cgctgaccca gaacgacatc aagaaggtgc tcgcctactc
      541 gacggtgagc cagctggcgt acatgttctt cgcgctcggg atcggggcct gggtggccgc
      601 catcttccat ctcgtcagcc acggcttctt caaggggctc ctcttcatgg gctcgggctc
      661 ggtgatccac ggcgcggcgg gcgagcagga catgcgattc atgggcggcc tgcgggagcg
      721 gatgccatgg acctatcgca cgatggtcgt cggctcactg gcgctcgccg ggatcccgat
      781 cttcgccggg ttcttcagca aggacgagat cctgggcgag gcgttcgggc gcggctacta
      841 cgtcttctgg gcggtcgggc tggtggtggc cttcatgacc gcgttctaca cgttccggat
      901 ggtctacatg accttccatg gccgatggcg cggcccggcc gacgcctggc agcacgtgca
      961 tgaatcggcg cccaccatgg tcgccccgct cgtcatcctg gccgtcccga cgatcctggt
     1021 cggcctcctg ctcggcctgc cgccggaggg cggcctgatc cacggctggc tcggcgacgt
     1081 cttccgcggc gcggaggagg cgggtgccgg catcctgccc ggctcgatcg cctcgggcgc
     1141 gcacgcaggc ttcagcttct tcgggatcgg cgggctgctc ctcctgctcg gagcgggcgt
     1201 tggcgccgcg ggcatctggc tggcgcaccg ctggtacgtg ctcgaccctg aggcgccggc
     1261 ccgctttgtg gctcgcatcc cgttcggcct cgggccgggg atgtacgcgg ccagcgtgaa
     1321 caagtactac gtggatgacc tctacctgct cgtctgggcg cgtggcggcg tgctcgtcgg
     1381 caacgcactg tggtggttcg acgccaaggt catcgacggg gccgtgaacg gcgcgggctg
     1441 gatcgcccag cgcctgggcg ggctccttcg ccgctcgcag accgggcacg tccagaacta
     1501 cggcctgggg atggccgccg ggctggtggt cgtcctgctc gcgttcctgg tgatccgcgg
     1561 atgagcggcc tgccgaccct gagcctggtc gtcttcacgc cgctgatcgg cgtgctgctg
     1621 atcctcttcg tgccaaagga caaccatcgg ctcattcgct ggatcgcgct cctggcggcg
     1681 ctcgccagcc tgggcttcag cctgttcctg ctcggctaca ggcccgacgg cgcggagttc
     1741 cagttccgcg aggacatctc ctggatctcc gccttcggga tgcgctacac gatcggcgtg
     1801 gacggcctct cggtcgtgct ggtgatgctc accaccgtgc tcagcgtggt gagcatcttc
     1861 tactcctggg acccgatcca gacccgggtc aaggagtact acgccacgat gctgctgctg
     1921 atgatcggga tgct
//
LOCUS       DATCQR010000045         4178 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_110651, whole genome shotgun sequence.
ACCESSION   DATCQR010000045 DATCQR010000000
VERSION     DATCQR010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4178
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110651"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..861)
                     /locus_tag="VIN32_01510"
     CDS             complement(<1..861)
                     /locus_tag="VIN32_01510"
                     /inference="COORDINATES: protein motif:HMM:NF014759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HEY8921282.1"
                     /translation="MADRRPILMLIDGPSIVYRAYFAMMQTPLTTSDGMLVTGIFGFL
                     NIVLRGMQDLKPDYAIVSFDLGKPQFRFDAYPDYKAGRPSMPDDLRAQFPIVRELVTM
                     MGMPIRELEGYEADDVIGTLALEAAAAGVDTYMVSGDLDGLQLVNDHIRLFTTRMGVA
                     NTVVYDEARVMERYGLRPDQMLDYKALKGDTSDNIPGVPGVGEKTAVSLLQQFGSLDN
                     LYEHLDEVKGKLRERLAEHRESAFLSRKVGRIVTDLPVPLELESARTGRYDRRAVAQR
                     LRELEFRSLID"
     gene            1100..1612
                     /locus_tag="VIN32_01515"
     CDS             1100..1612
                     /locus_tag="VIN32_01515"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HEY8921283.1"
                     /translation="MPTVPSTDPYAVLGIPRDATQQQVAGAHRRLAKRFHPDLHPDEP
                     TANHMRQVNEAWRILSSSARRAEYDAGHPTAGTPSSGHWAASRRPIRSSQPTTTRTWA
                     TWRATDAETRAAPRTIRQPGEVQLPPTRRPTPLEIGPRTFRDSGWAALIVAAVFLTLL
                     AIAVVVGKLA"
     gene            complement(1609..2289)
                     /locus_tag="VIN32_01520"
     CDS             complement(1609..2289)
                     /locus_tag="VIN32_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921284.1"
                     /translation="MTMQHTPHPDDERLAAYAGGDSDALADSSLASHVTTCDRCGPLV
                     DELSFLRGALADLPDLAPSRPLRLIPPVWDASAPASGPRSWLRRLTAPAMAAGAGLVL
                     VGAVGIGATGIAGLAGKSASFGAPQDAAASVSDREVGPVAGADSPSLAPSDMRGSAGS
                     PGSVAPQAMGSGTPATPRPTAEDNGRTDYETGSSSSEQPWLTLLIAGIGLFGISTALR
                     FSLSPRAG"
     gene            complement(2286..2915)
                     /locus_tag="VIN32_01525"
     CDS             complement(2286..2915)
                     /locus_tag="VIN32_01525"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY8921285.1"
                     /translation="MLPRDPARPASRTAPLPDEEAVIARAAAGDRTAFTQLIEHYQSA
                     CYGLAWRLLGDPDQAADAAQDAFVHAYDAIAKYRGGLFRSWLLRITANASYDILRRAQ
                     RKPTTTLPDAEEGEAELPDRSAADPEAEAGRSELYRYLDAALRLLPQDQRTAVVLCDV
                     YGMDYGEVADATESALGTVKSRIHRGRLRLRELMAEHRELFRNERRLDR"
     gene            3084..>4178
                     /locus_tag="VIN32_01530"
     CDS             3084..>4178
                     /locus_tag="VIN32_01530"
                     /inference="COORDINATES: protein motif:HMM:NF022100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NEW3 domain-containing protein"
                     /protein_id="HEY8921286.1"
                     /translation="MPTLSIHGRRRGATLAAIGLLLSSTLALAPAAVAAGLTLSTPYP
                     SVVVGPGTTVGFDITVAGNPNEQVGLSVGGVPSGWTATLRGGGYVVKGVQTDASGAAT
                     VRLELKIPATAGDGSSKVTVSAATGAARTTLPLTIQLSGAAAGDVTLTSDYPSLQGAS
                     GTSFTFNFNLHNDSAEDLTFAVTAVGPGGWEVNATLTGQAQAASAVVKATGSAGVSVS
                     VKPPTDVAPGTYDVNVRATVGDRTIDAKPTVVITGTNALTLSTPDQRLSNSGAAGSSI
                     TQQLVVHNDGTQPLENVVLSATAPTDWTVTFSPSATIASIAANQDATITATVVPSNDA
                     IAGDYVVSYKATAAIANGSVDIRMTIETSPL"
BASE COUNT          661 a         1460 c         1432 g          625 t
ORIGIN      
        1 gtcgatcagc gagcggaact cgagctcgcg aaggcgctgt gcaaccgcgc ggcggtcgta
       61 gcggcccgtg cgcgccgact cgagctccag gggcaccgga aggtcggtca cgatgcgccc
      121 caccttccgc gacaggaagg ccgactcccg gtgctccgcg agtcgctctc gcagctttcc
      181 cttcacctcg tccaggtgct cgtacaggtt gtcgagcgag ccgaactgct gcagcagcga
      241 caccgccgtc ttctccccta cccctggcac tccagggatg ttgtcggagg tatctccctt
      301 gagcgccttg tagtcgagca tctggtcggg acgcaggccg tagcgctcca tgacccgcgc
      361 ctcgtcgtag accaccgtgt tggcgacccc catccgggtc gtgaagagcc ggatgtggtc
      421 gttgaccagc tgcaggccgt ccaggtcgcc cgagaccatg taggtgtcca ccccggcggc
      481 ggctgcctca agggccaggg tcccgatcac gtcatcggcc tcgtagccct ccagctcgcg
      541 gatcggcatg cccatcatcg tcaccagctc ccggacgatc gggaactggg cgcgcaggtc
      601 gtcgggcatc gacgggcgac cggccttgta gtcggggtag gcgtcgaagc ggaactgcgg
      661 cttgcccagg tcgaagctga cgatggcgta gtccggcttc aggtcctgca tcccgcgcag
      721 gacgatgttg aggaagccaa agatcccggt cacgagcatg ccgtccgacg tcgtcagggg
      781 cgtctgcatc atcgcgaaat aggcccgata cacgatgctc ggcccatcga tgagcatcag
      841 gatcggtcgg cggtcggcca tcatcggagt ctagaccgat gggggcatcc ctcgacctta
      901 ccggcgaacc ttgccagatt cacgtggcac gaccgccatg cgcgacccgg gctcgccaag
      961 ccggccagat gcgtcacctg gcgtgaatct ggccgtgctg acctgcactg cggtcgtctg
     1021 gtgtgaattt gaccctcctc gcgtggcacg cggtcagtcc atgtgaatct ggcaggccca
     1081 gaactctaga atccgctccg tgccgaccgt gccgtcgacc gacccgtacg cggtgctggg
     1141 gatccctcgt gacgccaccc agcagcaggt cgcgggtgcc catcggcgcc tcgcgaagcg
     1201 cttccatccg gacctgcacc cggacgagcc gacggcgaac cacatgcggc aggtcaacga
     1261 ggcgtggcgc atcctctcgt cgagcgcacg gcgtgcggag tacgacgcgg gtcaccccac
     1321 cgccggcacg ccgagcagtg gacattgggc cgcgtcgcga cggccgatcc gttcctcgca
     1381 gcccacgacc acgcgcacct gggcgacctg gcgcgccacg gatgcggaga cccgcgccgc
     1441 ccctcgcacg attcgtcagc caggcgaggt tcagctgccc ccgacgcgcc ggccgacgcc
     1501 gctggagatc ggtccgcgca cgttccgcga ctcaggctgg gcggcactga tcgtggccgc
     1561 cgtcttcctg acgctgctcg cgatcgcggt cgtggtcggg aagctggcct agccggcgcg
     1621 gggtgagagg gagaagcgca gggcggtcga gatcccgaac aggccgatcc ccgcgatgag
     1681 cagggtcagc cacggctgct cggacgagga actgcccgtc tcgtagtcgg ttcggccgtt
     1741 gtcctctgcg gtgggccgcg gtgtcgctgg cgtgcccgaa cccatcgcct ggggcgcgac
     1801 agagcctgga gagccggcgc tgccgcgcat gtcgctcggt gccagcgacg gcgaatccgc
     1861 gccggcgact ggcccaacct cgcggtccga aacgctggcg gccgcatctt gcggagcacc
     1921 gaagctggct gacttgccgg ccaggccggc gatgcccgtc gccccgatcc cgacggcccc
     1981 caccaggacc agtccggccc cggcggccat ggccggcgcc gtcagtcgac gcagccacga
     2041 gcgcgggccg ctggcagggg cggaagcatc ccagaccggt gggatgaggc gcagggggcg
     2101 cgacggggcg aggtccggaa ggtcggcgag ggcgcctcgc aggaatgaga gctcgtcgac
     2161 cagcggcccg cagcgatcgc aggtcgtgac gtgcgacgcg agcgacgagt ccgcgagcgc
     2221 gtcggaatcg ccgcccgcat aggcggcgag cctctcgtcg tcgggatgag gcgtgtgctg
     2281 catggtcatc ggtccagacg cctctcgttc cggaagagtt cccggtgctc ggccatcagc
     2341 tcacgcaggc gaaggcgccc gcggtggatg cgcgacttga cggtcccgag cgcggactcg
     2401 gtcgcgtcgg cgacctcccc atagtccatg ccgtacacgt cgcacagcac cactgcggtg
     2461 cgctggtcct gtggcagcag ccgaagggcc gcgtcgagat agcgatacag ctcggagcgg
     2521 ccagcctccg cctccgggtc ggcggccgag cggtcgggca gctccgcctc gccctcctcg
     2581 gcgtcgggca gcgtcgtcgt cggcttgcgc tgggcacgcc ggaggatgtc gtagctggca
     2641 ttggcggtga tccgcagcag ccacgagcgg aagagcccgc cgcggtactt ggcaatggcg
     2701 tcgtacgcgt gcacgaatgc gtcctgtgcg gcatcggcgg cctggtccgg gtcgccgagc
     2761 aggcgccagg cgaggccata gcaggccgat tggtagtgct cgatcagttg ggtgaatgcg
     2821 gtgcgatcgc cagctgcggc gcgcgcgatc accgcctcct cgtccgggag gggcgcggtg
     2881 cgcgacgcgg gccgggcggg gtcgcgagga agcatcggac acctatgatc acattccgca
     2941 ggactgagaa gtattgcggt gtctcaacca ttcattgacc gcgaattggt tgcgcattcc
     3001 cgcatttctt gtcactttgc atctgcgccg agccgtcgcg ggaacgtgcc gccggttgcg
     3061 tgtgctgcgt tgaaggagac cgagtgccga ccctatcgat ccacgggcgc agacgaggtg
     3121 ccacgctggc cgccatcggc ctgctgttga gcagcaccct tgcgctcgcc cctgccgccg
     3181 ttgccgcagg gctgacactc tcgaccccct acccgtctgt tgtggtgggg cccggcacca
     3241 cggttggctt cgacatcacc gtcgccggca acccgaacga gcaggtcggc ctctcagtcg
     3301 ggggagttcc atccggctgg acggcaaccc tgcgcggcgg cggatacgtc gtgaaaggcg
     3361 tacagaccga tgccagcgga gccgcgaccg tccgcctcga gctcaagatc cctgccaccg
     3421 ctggcgatgg aagctcgaag gtgacggtga gcgccgcgac cggcgcggcc cggaccacgc
     3481 tgccgctgac cattcagctg agcggggcgg cggcgggcga cgtgaccctg accagcgact
     3541 atccctcgct gcagggagcc tccggcacga gcttcacctt caacttcaac ctgcacaacg
     3601 acagcgcgga ggacctcacc ttcgccgtca ccgcggtcgg gcccgggggc tgggaggtca
     3661 acgccacgct gaccggacag gcccaggcgg cgagcgccgt cgtcaaggcc accggcagcg
     3721 ccggcgtgtc agtctcggtc aagcctccga cggacgtcgc gccggggaca tacgacgtca
     3781 acgtgagggc gacggtgggc gatcggacca tcgacgccaa gccgacggtg gtgatcaccg
     3841 gcaccaacgc gctgacgctg agcacgcctg accagcgcct cagcaactcg ggcgcggccg
     3901 gcagctcgat cacccagcag ctggtggttc acaacgacgg cactcagcct ctcgagaacg
     3961 tcgtcctctc ggctaccgca ccgaccgact ggacggtcac cttcagccca tccgcgacga
     4021 tcgccagcat cgccgcaaac caggacgcca ccatcaccgc cacggtcgtg ccgtcgaacg
     4081 atgcgatagc gggcgactac gtggtcagct acaaggccac ggccgccatc gcgaatggct
     4141 ccgtcgacat ccgcatgacg atcgagacgt cgccgctc
//
LOCUS       DATCQR010000046         3669 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_33299, whole genome shotgun sequence.
ACCESSION   DATCQR010000046 DATCQR010000000
VERSION     DATCQR010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3669
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33299"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..693
                     /locus_tag="VIN32_01535"
     CDS             <1..693
                     /locus_tag="VIN32_01535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921287.1"
                     /translation="YLAVAIWLSEYVRSSVGYAWWHRFHMLAFVVFLLGTVHGLASGS
                     DSTSGWAVALYGGSVLVVSALVLVRAGRGLASRPGWAVATVVAATVLALAFFAVRGPL
                     YPGWNAVANNGNGNGASAVWLASHPIASGPPQDFVIDLQLQPVREDLLDARFDGGTPG
                     ELQLLLNQSSSLLALLFPDGWSCQGDLTITGQGAAVSRCIGSDGVALGVRLSGLQQAD
                     QAVTGRLEVSRA"
     gene            complement(700..1713)
                     /locus_tag="VIN32_01540"
     CDS             complement(700..1713)
                     /locus_tag="VIN32_01540"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY8921288.1"
                     /translation="MTFRRPALGILVVLLLAAVAYTGYVGYEGSRLLVEGGGSNPDCR
                     TPDELFGWTYEAINYDIADDALLTSRNSDQKNCTYTGAIAGNEVVTDDGIRLAGWYVP
                     AADGAGPTAATVVLVHGFGANKTSILEYGEALHATFNLVAFDLRNTQRSSGHQTTGGV
                     FEQKDLRAIIDWLERTKHPSHLGVLGNSLGAATAVAESRGDPRVEALALDSMHTRMRY
                     QVEQRLQHVGHPAYPGTWAVFLGARLRTGVDLGSVDAQDAIPDLVRLPVLLSHGTGDT
                     EDLPERTEAFYEDALAAGVQVELHWCEGAGHNASAGMPVRVCRDDFAAWMLDFFSRTL
                     SGA"
     gene            complement(1724..2557)
                     /locus_tag="VIN32_01545"
     CDS             complement(1724..2557)
                     /locus_tag="VIN32_01545"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8921289.1"
                     /translation="MTALARPISRIAPDTLRSAWRVFWYGGLMSYGMLFTWARPSVYI
                     PTVVGGPTFQLFFFAALGSYATDRSAAYFAIGNAVQVSALAGVFGMTMAISNERLFGT
                     LPALLATPANRVAVFAGRFMPFIVNGLLVSLYAFGLGVLFLGVRLAPGSIAVAGLALL
                     VTVFSCTAIGALQGAISLRLRDGLFGANLITMAILLFCGVNIPLRELPGWMQVVGNLL
                     PFTHGLEAVRRAADGAGLGQVGWLIGIEAIVGIVYAVLAFALFVYLERSARRNATLDV
                     R"
     gene            complement(2554..3342)
                     /locus_tag="VIN32_01550"
     CDS             complement(2554..3342)
                     /locus_tag="VIN32_01550"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8921290.1"
                     /translation="MSPRRTLRLIWFVTWFNVRMLLVSEFFILNAFIAPLIFATLAFF
                     LFRSGGGTAGQTLLYVALGAGMMGVWSTTIFGCGGAIAWQRWEGTLELLVNAPTRYDF
                     ILVGQTLGAVVFGFYGIVATLAWGVLLFGMPIQATYPLILPLSLLAAIVSLASLGMLI
                     ATTFVLYRHASALSNMLEYPIWLIAGLLVPVATLPFWVQPISWLLAPTWGMEAIRGAA
                     IGREPPFGAIVLCFALAVVYYLAARITLVFVLAKARRDATLALQ"
     gene            complement(3352..>3669)
                     /locus_tag="VIN32_01555"
     CDS             complement(3352..>3669)
                     /locus_tag="VIN32_01555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8921291.1"
                     /translation="DLKDAIAERTIVEIETFGVTDEAVERLRQDDDVSAINMEERDQS
                     QILLVQSDRGAHITQRLLSLLGDTRVGKVVAREPTLEDAYVHLIATTGDDDARAARAA
                     SVQ"
BASE COUNT          634 a         1223 c         1257 g          555 t
ORIGIN      
        1 tacctggcgg tcgcgatctg gctgtcggag tacgtgcgca gctccgtcgg gtatgcctgg
       61 tggcaccgct tccacatgct cgccttcgtc gtgttcctgc tcgggacggt tcacggactg
      121 gcgagcggca gcgattcaac ctcgggatgg gcggtggcgc tctacggcgg ctcagtcctg
      181 gtcgtgtccg cgctggtgct ggtgcgagcg ggccgggggc tcgccagtcg gccggggtgg
      241 gctgtggcga ccgtcgtggc ggccacggtc ctggccctgg ccttcttcgc cgtccgcggg
      301 cccttgtatc caggctggaa cgcggtcgcc aataacggca atggcaacgg tgcgtcagcg
      361 gtctggctcg cctcccatcc catcgccagc ggtccgccgc aagacttcgt gatcgacctg
      421 cagctacagc ccgttcgaga ggacctgctg gatgcccgct tcgacggtgg gacccccggt
      481 gagctccagc tcctcctcaa ccaatcgtcg tcactcctcg ccctcctctt tccagacggc
      541 tggtcctgcc agggagacct gacgatcacc ggtcagggcg cggccgtctc acgatgcatc
      601 gggtccgatg gagtcgccct cggggtgcgg ctgagcggcc ttcagcaggc ggaccaggcc
      661 gtgaccggac gtctcgaggt gtcgcgggcc tgagcgccgt caggctccgc tgagcgtacg
      721 gctgaagaag tccagcatcc aggcagcgaa atcgtcgcgg cacacgcgaa ccggcatgcc
      781 ggccgaagcg ttgtggcccg ctccctcgca ccagtgcagc tcgacctgga ctccggccgc
      841 gagggcgtcc tcgtagaagg cctcggtccg ctccggcagg tcctccgtgt cgccggtccc
      901 gtgggagagc agcaccggca gccgaaccag gtcggggatc gcgtcctggg cgtccaccga
      961 gccgaggtcg acgccggtgc gaagccttgc gcccaggaag actgcccagg tgcccgggta
     1021 ggcagggtgc ccgacgtgct gcagccgctg ctcgacctgg tagcgcatgc gggtgtgcat
     1081 tgagtcgagc gccagcgcct ccacccgtgg gtcgccgcgc gactccgcca cggccgtggc
     1141 cgccccgagt gagttcccaa gtacgccgag atggctcgga tgtttcgtcc gctcgagcca
     1201 gtcgatgatg gcgcgcaggt ccttctgctc gaagacgcca ccggtcgtct ggtgcccgct
     1261 gctgcgttgc gtgttgcgca ggtcgaaggc cacgaggttg aaggtggcgt gcaaggcctc
     1321 gccgtactcg aggatgctcg tcttgttcgc cccgaagcca tgcaccagga ccaccgtcgc
     1381 tgccgtcggg cctgcgccat cggctgcggg tacataccag cccgcgaggc ggatcccgtc
     1441 gtcggtgacg acctcgttgc cggcgatcgc gccggtgtag gtgcagttct tctggtcgga
     1501 gttgcgcgac gtcaggagcg cgtcgtctgc gatgtcgtag ttgatcgcct cgtatgtcca
     1561 gccgaacagc tcgtcaggcg tccggcagtc cgggttactg cccccgccct cgaccagtag
     1621 gcgcgacccc tcgtagccca catagccggt gtaagccacg gcggccagga gcagcacgac
     1681 aaggatgccc agagccggcc ttcggaacgt catggccgcg aggctaccgg acgtccaggg
     1741 tggcgttgcg acgcgcagat cgctcgagat agacgaacag cgcaaaggcg aggacggcgt
     1801 acacgatgcc cacaattgcc tcgatcccga tcagccagcc gacctgacca agccctgctc
     1861 catcggctgc ccggcggacg gcctctagcc cgtgggtgaa gggcagcaag ttgccgacca
     1921 cctgcatcca cccggggagc tcacgcagcg ggatgttcac gccgcagaac aggaggatcg
     1981 ccatggtgat caggttggcg ccgaacaggc catcgcgcag gcggagcgag attgcgccct
     2041 ggagcgcgcc gatggcagtg cacgagaaga cggtcacgag caatgccagg cccgccacgg
     2101 cgattgagcc aggcgcgaga cgcacgccga gaaacaggac ccccaatcca aaggcataca
     2161 gcgacaccag caggccattc acgatgaacg gcatgaagcg gccggcgaag accgccaccc
     2221 ggttcgcggg cgtagccagc agggcgggga gcgtcccgaa cagccgctcg ttggagatcg
     2281 ccatggtcat cccaaagacc cccgccagcg ccgacacctg gacggcgttg ccaatcgcga
     2341 agtacgccgc ggatcggtcg gttgcatacg accccagagc ggcgaagaag aagagctgga
     2401 aggttgggcc gcctacgaca gtcgggatat agaccgacgg acgagcccag gtgaagagca
     2461 tcccgtagct catcagtccg ccgtaccaga aaacgcgcca tgcagatcgg agcgtgtcgg
     2521 gtgcgatccg cgagatcggc ctcgccaggg cggtcactgc agtgccagcg tggcgtcgcg
     2581 ccgggccttc gccagcacga agacgagcgt gatgcgggcc gcgaggtagt acaccaccgc
     2641 cagcgcaaag cacaggacga tcgccccgaa aggaggctcc cgtccgatcg ccgccccccg
     2701 aatcgcctcc atcccccacg tcggtgccag cagccagctg atcggctgaa cccagaaggg
     2761 gagtgtggcg accgggacga gtaggccggc gatgagccag atcgggtact cgagcatgtt
     2821 gctgagggcg ctggcgtgcc ggtacaggac gaaggttgtt gcgatcagca tgcccaacga
     2881 ggccagcgac acgatcgccg ccagcagcga gagcggcagg atcagcgggt aggtggcctg
     2941 aatcggcatc ccgaacagga ggacccccca ggcgagggtg gcaacgatgc catagaaccc
     3001 gaagaccacc gccccaaggg tctggccgac caggatgaag tcgtagcggg tgggggcgtt
     3061 gaccagcagt tccagcgtcc cctcccagcg ctgccaggcg atggcgccgc cgcaaccgaa
     3121 gatggtggtg ctccagacac ccatcatccc cgcgccgagc gcgacgtaca ggagcgtctg
     3181 cccggccgtc ccgccgcccg accggaacag gaagaaggcg agcgtcgcga agatgagcgg
     3241 tgcgatgaac gcgttcagga tgaagaactc gctgaccagg agcatccgca cgttgaacca
     3301 tgtgacgaac cagatcagcc gcagcgtccg ccgagggctc atggggtcgc cctactggac
     3361 cgatgccgca cgggcggcgc gggcatcgtc atcgccggtg gtggcgatca ggtgaacgta
     3421 ggcatcctca agagtcggct cgcgcgccac taccttgccg acccgcgtgt cgccgaggag
     3481 tgagaggagg cgctgcgtga tatgcgcgcc gcggtcgctc tggaccagga ggatctggct
     3541 ctggtcgcgc tcctccatgt tgatggcgct gacatcgtcg tcctggcgca gccgctcgac
     3601 ggcctcgtcg gtcaccccga aggtttcgat ctcgacgatg gttcgctcgg cgatcgcgtc
     3661 cttcaggtc
//
LOCUS       DATCQR010000047         8328 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_157120, whole genome shotgun sequence.
ACCESSION   DATCQR010000047 DATCQR010000000
VERSION     DATCQR010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 8328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8328
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157120"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..115
                     /gene="rpmH"
                     /locus_tag="VIN32_01560"
     CDS             <1..115
                     /gene="rpmH"
                     /locus_tag="VIN32_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006778076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEY8921292.1"
                     /translation="KKRARKREHGFRARMASKGGRRVLARRRAKGREKLSA"
     gene            149..469
                     /gene="rnpA"
                     /locus_tag="VIN32_01565"
     CDS             149..469
                     /gene="rnpA"
                     /locus_tag="VIN32_01565"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEY8921293.1"
                     /translation="MLRRKADFDAIGRHGTVRSSRLLVLRSMRTPGSRTRVGLSTPRS
                     LGGAVQRNRVRRRLRDLLREQLNATEGGLDLLLIARSEAGTATYSELREALRSLLERS
                     GIGG"
     gene            466..672
                     /gene="yidD"
                     /locus_tag="VIN32_01570"
     CDS             466..672
                     /gene="yidD"
                     /locus_tag="VIN32_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018702056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEY8921294.1"
                     /translation="MRKIGLGLIIAYQRLFAWLPPSCRYTPSCSQYTYQAVERYGLLR
                     GSWMGVRRIGRCHPMHPGGYDPVR"
     gene            688..1848
                     /locus_tag="VIN32_01575"
     CDS             688..1848
                     /locus_tag="VIN32_01575"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HEY8921295.1"
                     /translation="MTLIGAGFLGIEHFTPPWDVFFLPLFNLLIASYRIIFSDFALAI
                     IVFTIVIRTLLAPLFVRQIRSQKEMQRMAPLIREVNRKHKGNRTKIAEETQALYREHG
                     VNPAAGCLPVVAQLPLLFALYQALIRASNVVTLSVKDAASDTFTQLQAALPGITRIGD
                     IKDAAGVVTQHQYAAPVAGACNLPQFEVANFTHFLPLNCQLIEPLQLTQRVNTHVSWL
                     FNLDLAQVDKVFGFVIPGLGFTISALAVVAALLQFIQVRMTSPKPNPDDPTSSTTNTM
                     TYLFPLLTIWWGGLFPSGLILYWVVYTAYLIAQQFRIMGWGNLFPLFGWQPGFAPAPE
                     TGVVSRPVRREPSPDNPGSDAAPPNQRAAGTPGGTSRPASRPTKKRRRGRKR"
     gene            1845..2840
                     /gene="jag"
                     /locus_tag="VIN32_01580"
     CDS             1845..2840
                     /gene="jag"
                     /locus_tag="VIN32_01580"
                     /inference="COORDINATES: protein motif:HMM:NF041568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HEY8921296.1"
                     /translation="MTFREFSGKNVEEAIRTAMKEYSADLAELDIEILSQGSRGILGV
                     GGEEARILAAPKSAVAAAETVRDTSPEPAEPIAPSRESIRAEAAPASAESEPTGEPMS
                     EQVADLGRSEAPPRRDGDGRSRGGRGRSDRDARGGRGDRPFRDRPPREPREPREDRPP
                     REPAPFIPGKPLEELTEKERGTLEEAKGVLEELLRLMEVPGTVEIATGGETARLNVRG
                     SDLGVLIGRRGEKLASLQHIVNLIVAKKEGEYHRIAVDVENYRGRREEQLRDVADRAA
                     KRVLQTGKIIQLEPMPAIERRIVHMALVENGKVRTQSVGVEPNRRIVILPATKVE"
     gene            2855..3298
                     /locus_tag="VIN32_01585"
     CDS             2855..3298
                     /locus_tag="VIN32_01585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921297.1"
                     /translation="MERPTAPLEATRAVGRLEAGGPWIGFGYRAGACRLVVGTAEGVL
                     DSPADPDLTLALAIAHFVGALEEAPPDLEATQSDLSALVSHLSRGEADPARQALLAEA
                     LDAIDDGLAGDAVAEKLAAARSSRTDAVDPVELLTVRAREMATGA"
     gene            complement(3295..3879)
                     /locus_tag="VIN32_01590"
     CDS             complement(3295..3879)
                     /locus_tag="VIN32_01590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921298.1"
                     /translation="MDFSKLNSNEKLAVYGAVAAIVGALLSFGGGGTFTLLTGVAMLI
                     IVFLPQFSPTTTLPGSKGSLMLIVGGIAGIAALLSLLAILTVLGAFALYGGLWFVGLL
                     LSTAGGLLMGWASWKEFQSEGGKFNLGTASSAPPASTAPPAAPPVSSAPPAEPMARPI
                     DEPMSPPAEPTSGSMDEPMRPMGGESNRDDEPPA"
     gene            complement(3987..4511)
                     /locus_tag="VIN32_01595"
     CDS             complement(3987..4511)
                     /locus_tag="VIN32_01595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921299.1"
                     /translation="MDLSKLSASERLAVYGAIATVIGGVIAAASYPGHWGVTWLGAIL
                     GLAMLAVIFLPQMSPGTTLPGKKGSLMVVLGGLAAVLMVYIFLTTIAFTFEGFDLQSA
                     LFLVAVAGAVVMGWAGWQAFQAEGGKLQIGAPGAVSAPPGAPTPPPIDAPATGQPPSM
                     PPPEANANGDDDQP"
     gene            complement(4606..4970)
                     /gene="ssrA"
                     /locus_tag="VIN32_01600"
     tmRNA           complement(4606..4970)
                     /gene="ssrA"
                     /locus_tag="VIN32_01600"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(5025..5483)
                     /gene="smpB"
                     /locus_tag="VIN32_01605"
     CDS             complement(5025..5483)
                     /gene="smpB"
                     /locus_tag="VIN32_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011232513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEY8921300.1"
                     /translation="MPDKTVAVNRRARHEYAVEETLEAGLALTGTEIKSIRAGRVNLA
                     EAYARIERGEAWLIGAHIAPYEQGNRNNHEPTRTRKLLLHRDQIAELVGRTQAKGFTL
                     VPLRLYIRNGMAKLEIGVARGKKAYDKRRTIAERDARRELERSTKERVRM"
     gene            complement(5635..6978)
                     /locus_tag="VIN32_01610"
     CDS             complement(5635..6978)
                     /locus_tag="VIN32_01610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEY8921301.1"
                     /translation="MSDQIPAPFPTTPPERPGAQRWSPVTWVVSLVLASMLGSLLFAG
                     GYLAAGGASRDGTCAAPTEAFAALCEAYGKLKQQYVDKLDDTQLAEGAIRGLFEYGVK
                     DPFSGYMSPDEYQRAKESLSGEFTGIGAEMAVKNTENPKDLTACPQLSETCTLVVVAP
                     LADSPAEAAGMQSGDVVLAVDGETVNGSTLQDEVGKVRGAPGTKVTLTLRRAGRTFDL
                     TITRAKIVQREVTSRMLDGKIGYIALHGFSQTSADEFRTSLQALLNDGAVSIVFDLRD
                     NPGGYIDAAQKIASEFIGSGLIFSQESSGGDKKPWEATGDGLATDPKLPLAVLVNGGS
                     ASASEIVAAALKETGRGVIIGQPTYGKNTVQVWTDLVDHGGVRITISRWFTPKHNSVA
                     PDGVQPDITVAVPDGTPPDQDPVLDRAITELTAPAASLPWRSGCIAGIAERRGDC"
     gene            complement(7056..7949)
                     /locus_tag="VIN32_01615"
     CDS             complement(7056..7949)
                     /locus_tag="VIN32_01615"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HEY8921302.1"
                     /translation="MRPFRFIAFAVVRAWQGFWRNAMMSLAATSTVVLMLVLLSGLLI
                     VLAGLDSGLHYIESKVGVTARLQDNLKQPQLDGLLADIRALPGVSKAEYISPEMALNR
                     LKQSYQEQGQTLDFGGAPISLYSSVEISLTNPSFADSIQTALGERPEVERITTRQVDY
                     AKLLSIIGYVRTVGFIALALVGLTVLFMIVNMIRIAVYSRSGEIEIMRLVGASDSFIR
                     WPFILEGILCGVIGAVVTIILVAVAWEPIKPIMVRVFQMPTAVSTDFLAVLSAVILGV
                     GLGVGAVGSWISVRTNLSSVT"
     gene            complement(7953..>8328)
                     /locus_tag="VIN32_01620"
     CDS             complement(7953..>8328)
                     /locus_tag="VIN32_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003869432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY8921303.1"
                     /translation="EVTGATNGEIRRRVPRLLNLVGLHEHDDHLPAQLSGGEQQRTAI
                     ARALVHDPAMLIADEPTGNLDPVTSWDLIQLLIQINTMGTTVLMATHNQEIVNAMRRR
                     VLALEHGTLVRDESGAAYEREY"
BASE COUNT         1333 a         2779 c         2860 g         1356 t
ORIGIN      
        1 caagaagcgc gcccggaagc gggaacacgg cttccgcgct cgcatggctt cgaagggtgg
       61 tcgacgcgtg cttgcccgtc gtcgggcgaa ggggcgcgag aagctcagcg cctgacggcg
      121 acgaggaccc gaggtgccgc cccttgagat gcttcggcgc aaggcggact tcgacgcaat
      181 cggtcgccac gggacggttc gctccagtcg gctcctggtc ctgcgatcga tgcggacgcc
      241 ggggtcgagg acgagggtcg ggctgagcac tccgcgatcg ctcggtggcg cggtccagcg
      301 caatcgggtg agacgtcggc tgcgcgatct gctccgcgag cagctgaacg ccacggaagg
      361 gggcctggac ctgctgctga tcgcgcgatc ggaggcgggg acagcgacct actcggagct
      421 gcgagaggca ttgcgatcgc tccttgagcg atccggaatc ggtggatgag gaagatcggg
      481 ctcgggctga tcatcgcgta ccagcggctc ttcgcctggc tgccgcccag ctgccgctat
      541 acgccgagct gctcgcagta cacctaccag gcggtcgagc gctatggcct cctgcggggg
      601 tcctggatgg gtgtccgccg cattggccgc tgtcatccga tgcacccagg tggctatgac
      661 cccgttcgct agagagagca ccagaccttg acactcatcg gcgccgggtt cctggggatc
      721 gagcacttca cgccgccgtg ggatgtcttc ttcctgccgc tgttcaacct cctgatcgcg
      781 tcgtatcgga tcatcttcag cgacttcgcg ctcgcgatca tcgtcttcac gatcgtcatc
      841 cggaccctgc tggcccctct cttcgtgcga cagatccggt cgcagaagga gatgcagcgg
      901 atggcgcccc tcatccgcga ggtgaaccgc aagcacaagg gaaaccggac caagatcgcc
      961 gaggagacgc aggcgctgta ccgggagcat ggcgtcaatc cagcggccgg ctgcctcccg
     1021 gtggtggccc agcttccgct cctgttcgcc ctctaccagg cgctcatccg cgcctcgaac
     1081 gtggtcactc tctcggtcaa ggacgccgct tcggacacct tcactcagct gcaggctgcg
     1141 ttgccgggga tcaccaggat cggtgacatc aaggacgccg cgggtgtggt cacccaacac
     1201 cagtacgcgg cgccggtggc cggtgcctgc aacctgccgc agttcgaggt cgccaacttc
     1261 acccacttcc ttcccctgaa ctgccagctg atcgagcccc tccagctcac ccagcgggtc
     1321 aacacccatg tctcgtggct cttcaacctg gacctggcgc aggtcgacaa ggtcttcggg
     1381 ttcgtcatcc cgggccttgg cttcacgatc tcggcgcttg ccgtcgtggc cgcgctgctc
     1441 cagttcatcc aggtccgcat gacgagcccc aagccgaacc ccgacgaccc cacttcgtcg
     1501 acgaccaaca ccatgaccta cctgttcccg ctactgacga tctggtgggg cggcctcttc
     1561 ccgagcggcc tgatcctgta ctgggtcgtc tacaccgcct acctgatcgc ccagcaattc
     1621 cggatcatgg gctgggggaa cctgttcccg ctcttcggct ggcagcctgg cttcgcgccg
     1681 gccccggaga ccggggtcgt cagccgaccc gtgcgtcgcg agccatcccc agacaacccg
     1741 gggagtgacg ccgcgccgcc gaaccaacga gccgccggca cgccgggggg caccagccgc
     1801 cccgcgtccc gccccaccaa gaagagacgc cgaggacgaa agcgatgaca ttccgagagt
     1861 tcagtggcaa gaacgtcgaa gaggcgatcc gcaccgccat gaaggagtac agcgcggatc
     1921 tcgccgagct ggacatcgag atcctgtcgc aggggagccg cggcatcctc ggcgtgggag
     1981 gcgaggaggc acgcatcctg gccgcgccga agtcggcggt cgcggccgcc gaaacggtgc
     2041 gggacacgtc ccctgagccc gcagagccga tagcgccatc acgtgaatcc atccgggcgg
     2101 aggccgcccc agcctctgcc gaaagcgagc caacgggcga gccgatgagc gagcaggtcg
     2161 ccgatctcgg acgctccgaa gcgcctccgc gacgcgatgg agacggcaga tcccgtggcg
     2221 ggcgcgggcg ctcggatcgc gacgcgcgcg gtggtcgagg cgatcgtccc ttccgcgacc
     2281 gtccccctcg cgagccccgc gagcctcgcg aggacaggcc tccacgcgag cccgcgccgt
     2341 tcatccccgg caagccgctc gaggagctga ccgagaagga gcgcggcacg ctggaggagg
     2401 cgaagggcgt gctggaggag ctgctgcgcc tgatggaggt gcctggcacc gtcgagatcg
     2461 caaccggcgg tgagacagcg cgcctgaacg ttcgaggctc ggacctgggt gtgctgatcg
     2521 gcaggcgcgg cgagaagctg gccagcttgc agcacatcgt caacctgatc gtggccaaga
     2581 aggagggcga gtaccaccgc atcgcggtgg acgtggagaa ctaccgggga cgccgcgagg
     2641 agcagcttcg cgacgtcgcc gaccgggccg ccaagcgggt cctgcagacc ggcaagatca
     2701 tccagctcga gccgatgccc gcgatcgagc gccgcatcgt gcacatggcg ctggtcgaga
     2761 acggcaaggt tcgcacccag agcgtgggtg tcgagccaaa ccggcggatc gtgatcctgc
     2821 ccgccaccaa ggtcgaatag ccgagccgtc cgccatggag cgccccacgg cgccgcttga
     2881 ggccactcgc gccgtcggcc ggctcgaggc cggtggtccg tggatcgggt tcggctaccg
     2941 ggccggggcc tgtcgcctgg tcgtggggac cgccgagggt gtgctcgatt cccccgccga
     3001 ccccgatctc accctggcgc tcgcaatcgc ccatttcgtg ggagcgctcg aggaggcgcc
     3061 ccccgacctc gaggccacgc agtcggacct gtcggccctg gtgagccacc tcagcagggg
     3121 agaggcggat ccagcgaggc aggcactgct ggccgaggcg ctggacgcca tcgacgacgg
     3181 cctcgcaggc gatgccgtgg ccgagaagct ggcggcagcc aggtcatcgc ggaccgacgc
     3241 ggtcgacccg gttgagctcc tgacggtgag ggccagggag atggcgaccg gggcttaggc
     3301 cggcggctcg tcgtcccggt tgctctcacc gcccatcggg cgcatcggct cgtccatcga
     3361 acccgacgtc ggctcggccg gtggggacat cggctcgtcg atcggccgcg ccatcggctc
     3421 agcgggcggg gctgacgaga caggcggtgc cgccggggga gccgttgacg cgggaggagc
     3481 gctgctggct gtgcccaggt tgaacttgcc gccctctgac tggaattcct tccaactggc
     3541 ccagcccatc agcagtccac cagcggtgct gagcagcagg ccgacgaacc acaggccgcc
     3601 gtacagggcg aatgccccga ggacggtgag gattgcgagc agcgacagca gcgccgcgat
     3661 gccggcgatg ccaccgacga tgagcatcag gcttcccttc gagccaggga gcgtggtggt
     3721 cggtgagaac tgcggcagga agacgatgat gagcatggct acaccggtca gcagggtgaa
     3781 ggtgcccccg ccgccgaagc tcagcagcgc accgacgatg gccgcgactg caccgtagac
     3841 cgcgagcttc tcgttggagt tgagcttgga gaagtccatc tgaatcaaac gcctccctat
     3901 gtcatcgcgg cgacgatgcg tggagcgcgc tgggcgtgcc gcatgggcgc gaccttagca
     3961 caggcgatgg ccgcttcata ggccgatcag ggctggtcgt cgtccccgtt tgcgttggcc
     4021 tcgggcggcg gcatgctggg cggttggccg gtcgccggtg cgtcaatcgg tggaggcgtc
     4081 ggcgcgcctg gcggggcgga caccgcgccg ggtgcgccga tctgcagctt gccgccctcc
     4141 gcctggaagg cctgccagcc ggcccagccc atgaccacgg cgccggccac ggcgaccagg
     4201 aagagcgccg actggaggtc aaagccctcg aaggtgaagg cgatggtcgt gaggaagatg
     4261 tagaccatga ggacggcggc cagtccgccc agcaccacca tcaggctccc cttcttcccc
     4321 ggcagcgtcg tgccgggcga catctgcggc aggaatatca ccgccagcat ggcgaggccg
     4381 aggatggctc cgagccaggt cacgccccag tgcccggggt agctggctgc cgctatcacg
     4441 ccgccgatga cggtggcaat ggccccgtag accgcgagcc gttccgatgc gctgagcttg
     4501 gagaggtcca tgagtgccat cgctccttca tcggcggcct gtgcagggga gtgacgggcc
     4561 gcgtgcaggc acggtagcac gcgaaatggg ccgcggaagg tgggttggtg gagatggggg
     4621 agaatcgaac tccccgtcca gaaccgagtc gccgggaacg tactacagat atttccggcc
     4681 tatttcgaga ttcgcctgcg ggcccccgac cggcggggtg ctccgctcgc tatccggtta
     4741 gcctttcggc cctttgacgc cacttaccgg agtcggcggc gccgcatctc gccttgtgac
     4801 gccttcacgg cccggcgaga gagggccgtg tcagcgctcg tcgctactta ggcggcgaga
     4861 gcgtaactgt tagtgccagt tactggcttg cccgcggttt tacgaggcca tgcgggcgac
     4921 ctcgacctgc agttcccggt ccctgagccc tgtcgaaacc tgacatcccc acggaccgat
     4981 cgaggattca tggtagcacg ggccccccgc gagcccgatc agcgctacat gcgcacgcgc
     5041 tccttggtcg atcgctcgag ctcgcggcgc gcatcccgct cggcgatggt gcggcgcttg
     5101 tcatacgcct tcttgccgcg cgcaaccccg atctcaagct tggccatgcc gttccggata
     5161 tacagcctga gggggacgag ggtgaagccc ttggcctggg tcctcccgac cagctcggcg
     5221 atctggtccc gatgcagcag cagcttcctg gtccgggtcg gctcgtggtt gttccggttc
     5281 ccctgctcgt acggggcgat gtgcgccccg atcagccacg cctctccccg ttcgatccgg
     5341 gcgtaggcct cggcgaggtt gacacggccg gcacggatcg acttgatctc ggttccggtc
     5401 agcgcgagtc cagcctcgag cgtctcctcg acggcgtact cgtggcgggc gcggcggttg
     5461 acggcaacgg tcttatcggg catcggaggc tgcgtgaggt cgagaaatga aagagccgac
     5521 cgtatcggcc ggctctctcg tcgctaccac acgaaggggt gctagcgcac cgcctcggtg
     5581 gtggcgtgac aggaactact cactgcacac cacctccttt cgttgggcgg gatgctagca
     5641 gtcgccccgt cgctctgcaa tgcccgctat gcagccgctc cgccagggga gcgaggccgc
     5701 aggcgccgtg agctcggtga tggcgcggtc gagaaccgga tcctggtcgg gtggcgtccc
     5761 atcgggaacc gcgaccgtga tgtcgggctg cacgccgtcg ggagcgaccg agttgtgctt
     5821 tggagtgaac cagcgcgaga tggtgatccg cacgccgccg tggtcgacca ggtcggtcca
     5881 cacctggacc gtgttcttgc cgtaggtcgg ctgaccgatg atcacgccgc ggccggtctc
     5941 cttgagggcc gcggccacga tctcggaggc cgatgcgctg ccgccattga cgaggacggc
     6001 cagcggaagt ttcgggtcgg tggccagccc gtcgccagtc gcctcccagg gcttcttgtc
     6061 tccgccggac gactcctggc tgaagatcag ccccgagccg atgaactccg acgcgatctt
     6121 ctgtgcggcg tcgatgtagc caccggggtt gtcccgcagg tcgaagacga tcgagacggc
     6181 gccatcgttg agcagcgcct ggagcgaagt cctgaactca tccgcgctcg tctggctgaa
     6241 gccgtgcagc gcgatgtagc cgatcttgcc atcgagcatc ctgctggtga cttcgcgctg
     6301 gacgatcttt gcccgggtga tggtcaggtc gaaggtgcgc ccagctcggc ggagcgtcag
     6361 ggtgaccttg gtccccggcg caccgcgaac cttgccgacc tcgtcctgca gcgtcgaccc
     6421 gttgacggtc tcgccatcca ccgccagcac gacatcgccg gactgcatgc ccgcggcctc
     6481 ggccggggag tcggccagcg gcgcgacgac caccagggtg caggtctccg acagctgcgg
     6541 gcatgcagtc aggtctttgg ggttctcggt gtttttgact gccatctcgg cgccaatccc
     6601 ggtgaattct cctgagagcg attccttggc gcgctgatac tcgtcggggg acatgtagcc
     6661 gctgaacgga tccttgactc cgtattcgaa gagcccccgg atggcaccct cggccagctg
     6721 ggtgtcgtcc agcttgtcga cgtattgctg cttcagcttg ccgtacgcct cgcatagggc
     6781 cgcgaaggcc tccgtcggcg ccgcgcacgt cccgtcgcgg cttgccccac ccgcggcgag
     6841 atagccgccc gcgaagagga gcgacccgag catgctggcg aggaccagcg acacgaccca
     6901 cgtgaccggg gaccagcgtt gggcgcctgg gcgctcgggc ggcgtggtag ggaatggggc
     6961 ggggatctgg tcgctcatcg gcggcggagt gtagcaccgg gccggcgggg gcccggttac
     7021 gcctccatta cgtttcgctt accgcagcag ccccgtcagg tcaccgagga caggttggtt
     7081 cggacgctga tccagctgcc cacggctccc actcccaggc ccacgcccag gatgaccgcc
     7141 gagaggacgg cgaggaagtc cgtgctgacg gcggtcggca tctggaagac ccgcaccatg
     7201 atcggcttga tcggctccca ggcaaccgcc accaggatga tggtgacgac ggcgccgatg
     7261 actccgcaca ggatcccctc gaggatgaat ggccagcgga tgaacgagtc gctggccccc
     7321 accagtcgca tgatctcgat ctcgccgctg cggctgtaca ccgcgatccg gatcatgttc
     7381 acgatcatga acaggaccgt gaggcctacc agcgccagcg cgatgaaccc aaccgttcgg
     7441 acgtagccga tgatcgagag cagcttggcg tagtcgacct ggcgggtggt gatgcgctcc
     7501 acctccggtc gttcacccag tgcggtctgg atggagtcgg cgaagctcgg gttggtcagg
     7561 gagatctcga cgctggagta gaggctgatc ggggcgcccc caaagtcgag cgtctgaccc
     7621 tgttcctggt aggactgctt cagccgattc agcgccattt ctgggctgat gtattcggct
     7681 ttgctcactc ccggcagggc ccggatgtcg gcaagcaggc cgtcgagctg gggctgcttc
     7741 aggttgtcct ggaggcgggc agtgaccccg accttgctct cgatgtagtg caggccgctg
     7801 tcaaggccgg ccagcacgat cagaagcccg gagaggagga ccagcatcag caccacggtg
     7861 gaggtcgccg cgaggctcat catcgcgttg cgccagaatc cctgccaggc gcggaccacc
     7921 gcgaaggcga tgaagcggaa ggggcgcatc ggctagtact cgcgctcgta tgccgcgccg
     7981 gactcgtcgc ggaccagggt gccatgctcg agcgccagca cacgccgccg catggcattg
     8041 acgatctcct ggttatgcgt cgccatcagg acggtggtgc ccatcgtgtt gatctggatc
     8101 aggagctgga tgaggtccca gcttgtgacg ggatcgaggt tgccggtcgg ctcgtccgcg
     8161 atcagcatcg ccgggtcgtg gaccagtgcc cgggcgatgg cggtgcgctg ctgctccccg
     8221 ccggagagct gagcgggcag gtggtcgtcg tgctcgtgca ggccgaccag gttcaggagc
     8281 cgcggcacac ggcgccggat ctccccgttc gtcgcgccgg tcacctcc
//
LOCUS       DATCQR010000048         8425 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_33448, whole genome shotgun sequence.
ACCESSION   DATCQR010000048 DATCQR010000000
VERSION     DATCQR010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 8425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8425
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33448"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /locus_tag="VIN32_01625"
     CDS             complement(<1..485)
                     /locus_tag="VIN32_01625"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY8921304.1"
                     /translation="MADQIRRRISTGDLRPGQRIESSRKLAAELRVSLPVLREALAAL
                     SYLGMIEVRHGVGVFVARRQPAARVLRVSHRRAHRAELHQLRATIAAEVAARAASHKR
                     SDRQQLDLHLLLQERHRSVLAGEPQAFVEADLDLHAFVAGVARSPLHAALERMAGVGL
                     RT"
     gene            716..1570
                     /locus_tag="VIN32_01630"
     CDS             716..1570
                     /locus_tag="VIN32_01630"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEY8921305.1"
                     /translation="MPISHETDLAFAVALADQAGKLLTASYEQAMKVDRKSKRDVVTN
                     VDYASEALVIKAIRDRFPGDAILAEESGRHERADQAEQSGSARSRGRWSRRTWVIDPL
                     DGTVNYANGIPFFCVSIGLVEEGVPVVGVVLDPLRGDCYAATAGGATTLNGEPVRASE
                     KTELGDFVVSLAIIGRGGIARERRVAHEIRIGRRMGSAALALAYIASGRFDAYVQNGG
                     LSLWDIAAAGLIAERAGATVSDLHGGAWWNVAKRGARLSIVAAPQPHHGKLLDLLRAA
                     RTTVETRR"
     gene            complement(1567..2238)
                     /locus_tag="VIN32_01635"
     CDS             complement(1567..2238)
                     /locus_tag="VIN32_01635"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HEY8921306.1"
                     /translation="MDRILRGADGWQDVLAGALARTTRPIPADPRFAPRAAVSGSNLP
                     RYDRADFPSARAAATLLLLYPGIDGELTLLLTVRHSALRDHAGEISLPGGAADPVDAS
                     LEATAIREAWEEVGVEPATVRIVGVLDDIWIPVSNFELRPFVGSTAARPTFAPHTDEV
                     AEIVELPLRHVLADGAVSEELIEGPGWQLRAAAYRYAGHRIWGATARTLAMFAAVLQG
                     AQLDA"
     gene            2237..2680
                     /locus_tag="VIN32_01640"
     CDS             2237..2680
                     /locus_tag="VIN32_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921307.1"
                     /translation="MAFPAPASRDALARAALYRTRVLVNRTPHFTTRARREANRGLDL
                     LDAQLGLNQVNLPEAVRGVELMIRATPSLAFGLLRDPAFVDRFGAALRHLGLRGIAQR
                     LDEVPHSVMAEPIPGPTAGRRHRDELPSDERVDAEGNPLPPLPGF"
     gene            complement(2677..3477)
                     /locus_tag="VIN32_01645"
     CDS             complement(2677..3477)
                     /locus_tag="VIN32_01645"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8921308.1"
                     /translation="MTNLTLEAVTYRYPHGGTLALDGIELHIGTGERVALIGQNGSGK
                     TTLVRHLNGLLRPTSGRVLHDGRDVARQTVAQLARAVGLAFQDPDRQIFAGSVRREVE
                     FGPRNLGMSGGELRTAVDTALAVVGLGEEADSNPYDLGASRRKLLALASVQAMGTPVL
                     VLDEPTTGQDMRGVEIVKRVIAAAHAAGRTVIAISHDMEFVAASFDRVVVLREGRVIL
                     DGGPASVFAEASWDALRSTCLEPPLPAVIGSHLRVGDTPTDDALLAAL"
     gene            complement(3474..4316)
                     /locus_tag="VIN32_01650"
     CDS             complement(3474..4316)
                     /locus_tag="VIN32_01650"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8921309.1"
                     /translation="MAALTLVLESLTYRYAGALRPSLHEVSLELREGEVLGLVGASEA
                     GKTTICLVASGLAPRTVGGTLSGRLLLDGEDTAPLAMHDLAGRIGIAFASPATQLSGV
                     AATVYEEVAFGPMNLGVPRSELIARTEEALSALRIESLADRDPAQLSGGQQQLVAIAG
                     LLALRPAHLVLDEPTAQLDPAGTAMVAAALAGLAANGASILVAEQKTDLLAGICSRVA
                     AIDAGRVVLDGPAVEVLADPRLTDLGVAPPAAVRLRRAAAEANLPAASLARLADAIAE
                     VPPR"
     gene            complement(4304..5185)
                     /locus_tag="VIN32_01655"
     CDS             complement(4304..5185)
                     /locus_tag="VIN32_01655"
                     /inference="COORDINATES: protein motif:HMM:NF014420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HEY8921310.1"
                     /translation="MPDPLGAGEPLQGALPAGGAGARGAFGLTDRLPSFLGAPGPTSV
                     HRLNPLTKAALAAVTTVLAVLSGGLLGPAILAVVAVVLPALIGRILGRLLRTALLLSL
                     PIAISVLLVNLFFFPAGREILFQVGPITATAEGLGFALETLARIGAISGAITLFYLTT
                     PPSELVLDLERRGVSPRIAFVALASVQTVPALVERAATITAAQRARGLDTEGSVWRRL
                     RGVLPLAGPVLLGSITEVEERTMALEARGFTHPGRRTLLWWPADSARQRVARWLLVLA
                     VPLLIVARALGWLGWLR"
     gene            complement(5101..6342)
                     /locus_tag="VIN32_01660"
     CDS             complement(5101..6342)
                     /locus_tag="VIN32_01660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921311.1"
                     /translation="MDSARWTRIAIGVVVGALVGLGLYATGQVWPLSAAVGVIVADVV
                     YAGLSLAQTGEWGPMRVAAGLVAFAAATYIGIIGFGDFGDANAVDPNTFANTKAANSW
                     ALILTVAMVIGAVVTYGLELAQTGRLASLSRQFGTRTYLLMAVGIGINVILGQTVASA
                     LKIPIYLDSIGTILVGVLCGPVAGALTGGLGNILWSYVIPPPFQYQPAAAFAIVAVAI
                     GVIAGLLGRAGFLRPRPNRPPAELLAGGAVTVVLVGGLTWAAALAYTRIFETKIDLFP
                     DPAKTDLIFVLLGYLAVALVALAVVGLFALLLVRRDLTAAYVVVAGVFTGIVAALISA
                     PIAAGVFGGVTASGTDFLVAAFRRAGADVYAATTGQGLISDPIDKVTTFFTVYLILSA
                     LANRFKARFPQGEQVLEEPSA"
     gene            complement(6347..7357)
                     /locus_tag="VIN32_01665"
     CDS             complement(6347..7357)
                     /locus_tag="VIN32_01665"
                     /inference="COORDINATES: protein motif:HMM:NF013335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HEY8921312.1"
                     /translation="MQASPTDAEPRPVILDVDTGIDDMIALLIAATAPELNLRGVTCV
                     AGNVEIHHVARNTGKILRMVGRGDVPVSIGAARPLHRRLRTAPDTHGPTGTGYVELTG
                     EDPDLGATDYTAVPAPRYLAAHIQRRPGQLTVVALGPLTNIASAALNGVDLAGGLREL
                     IWMGGALEERGNTTETGEWNACVDPEAAEAVLAAGAISRIFPLDATQDVIFTLEDLDG
                     LPGTPLSAIVRDAVRFYISFHREAEGIDGCYLHDPVTLIGSLLRPDLVTDSVDERLAC
                     DTGSNPHLAGSLYRSRDENRRAVNVATALDAEGMHRELLERLAHAVSVTTAQVQALHE
                     GG"
     gene            complement(7376..8278)
                     /locus_tag="VIN32_01670"
     CDS             complement(7376..8278)
                     /locus_tag="VIN32_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012796092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HEY8921313.1"
                     /translation="MTRPEVRIYEVGPRDGLQAEATVVSTDAKLAFVAMLADAGLREI
                     ETTSLVSPKAIPQMADADELLTRLERHPGVRYPVLVPNARGLERAISAEVDAICLMTA
                     ATDSYVQHNIGMSVDESLAAFGPIAAEARRRGWWIRAYVSTAFGCPYEGEVDERAVVS
                     VAERLLRLGVDELSISDTLGVAAPVDVARVLAALGAGGIGPEVVAMHLHDTRGTALAN
                     VMAAIELGVRCFDSSAGGTGGSPYAPGSAGNLATEDLVYLLDREGVRTGVNLDGILAA
                     ARFIAGELGTEVGSKVGRAGAWPQ"
     gene            complement(8291..8416)
                     /locus_tag="VIN32_01675"
     CDS             complement(8291..8416)
                     /locus_tag="VIN32_01675"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEY8921314.1"
                     /translation="MIEAMKMEHAVVTPLTGTVTHVAVKVGQQVQRGDLLAEVSA"
BASE COUNT         1320 a         2972 c         2948 g         1185 t
ORIGIN      
        1 gtcctgaggc ccactccagc catgcgctcc agcgcggcat gcagcggact gcgagcgact
       61 ccggcgacga aggcgtgcag gtccaggtcg gcctcgacga aggcttgcgg ctcacccgcc
      121 aggacagacc gatggcgctc ctggagcaac aggtggaggt caagctgctg tcggtccgag
      181 cgtttgtgcg acgctgcccg ggcagcgact tcggcggcga tcgtcgctcg caactggtgg
      241 agctcggccc tgtgcgctcg ccgatgcgag actcgcagca cgcgcgcggc aggctgtcgg
      301 cgagccacga agacgccgac cccatgtcgc acttcgatca tcccgaggta cgacagcgcc
      361 gccagagctt ccctcaggac cggcaggctg acccgcagct ccgctgccag cttcctcgag
      421 gattcaatgc gctggccggg acgcagatca ccggtgctga ttcgtcgtct gatctgatcg
      481 gccacttgcg ccgccaacgg gcgccgagct gccggataca ggtcagcagg cagtcgccag
      541 aagtccagcc gcggcgccga acgcttcgga attggtctcc aatggcctga ccccgtggcc
      601 cggctgcgtt tcatgacctc ggtccccaac tgttcaagtc atctgaacag tcccatggct
      661 tgccttactg gtggatgagc gtggacttac cgcagctcgg ctacgatcga ttcagatgcc
      721 catctcccac gagacagacc ttgcgtttgc ggttgccctg gcagaccagg ctggcaagct
      781 gctgacggcc agctacgagc aggcaatgaa ggtcgatcgg aagagcaagc gggatgtcgt
      841 caccaacgtg gactacgcca gcgaagccct tgtgatcaag gccatccgcg accggttccc
      901 gggcgacgcg atcctcgccg aggagtccgg ccgccacgaa cgcgccgatc aggccgagca
      961 atccggctcc gcacgaagca ggggccgatg gagccgacgg acctgggtca tcgacccgct
     1021 cgacggcacc gtgaactacg ccaacgggat cccgttcttc tgtgtctcca tcggtcttgt
     1081 cgaagagggc gtcccggtcg tcggcgtggt cctcgacccg ctgcgaggcg actgctatgc
     1141 cgcgacagcc ggcggtgcca cgacgctcaa cggtgagccg gtgcgcgcca gcgaaaagac
     1201 ggagctgggg gacttcgtgg tcagcctcgc catcatcggt cgagggggca ttgctcgcga
     1261 gcggcgcgtg gcgcacgaga ttcggatcgg gcgtcggatg gggagcgcgg ccctggcttt
     1321 ggcgtacatc gcctcgggcc gcttcgacgc ctacgtccag aatggcgggc tatcgctgtg
     1381 ggacatcgcg gcggcaggcc tcattgccga gcgggctggg gcaacggtct cggaccttca
     1441 cggcggggcg tggtggaacg tcgccaaacg gggtgcgcgg ctgagcatcg tggccgcccc
     1501 gcagccgcac cacggaaagc tgctcgacct cctccgagca gcccgaacga cggtcgagac
     1561 tcggcgctag gcatcgagct gcgccccttg cagtaccgcg gcgaacatcg ccagcgtgcg
     1621 cgcggtggcg ccccagatcc gatggccggc gtaccgatac gccgcggcgc gcagctgcca
     1681 accgggcccc tcgatcagct cctcgctgac tgcgccgtca gccaggacgt ggcgcagcgg
     1741 gagctcgacg atctcggcca cctcatcggt gtggggagca aaggttggac gggcggcggt
     1801 gcttccgacg aagggtcgca gctcgaagtt gctgaccggg atccagatgt cgtcgaggac
     1861 gccgacgatc ctcaccgtcg ccggctcgac tccgacctct tcccaggcct cgcgaatggc
     1921 ggtcgcctcc aacgaggcgt ccacggggtc ggcggcaccg ccggggagcg agatctcgcc
     1981 ggcgtggtca cgcaatgcgg aatgacggac ggtgagcagc agcgtcagct cgccatcgat
     2041 tcccggatac aggagcagca gggttgcggc ggcgcgcgcc gacgggaagt cggcacggtc
     2101 gtagcggggg aggttggacc cgctgaccgc ggcgcgtggc gcgaacctcg ggtcggcagg
     2161 gatcggccgc gtcgtccgcg ccagggcgcc ggccagcaca tcctgccatc cgtccgcgcc
     2221 gcgtaagatt cggtccatgg cctttcccgc tcccgcgagt cgcgacgccc tggcccgcgc
     2281 tgccctgtat cggacgcgcg tgctggtcaa tcgcacgccg cacttcacca cgcgggcgcg
     2341 gcgcgaggca aatcgtggcc tcgacctcct cgacgctcag ctggggctga accaggtcaa
     2401 tctgcccgaa gcggtgcgcg gcgtcgagct gatgatccgc gccacaccct cgctggcatt
     2461 cggcctgctg cgagatcctg cgttcgtcga ccgcttcggt gccgcgctgc gccacctcgg
     2521 gctgcgcggg atcgcccagc gcctggacga ggtcccgcac tcggtcatgg ccgagccgat
     2581 ccctggcccc accgcggggc gtcgtcaccg cgacgagctg ccgtccgacg agcgggtcga
     2641 cgccgagggg aatccgcttc cgccgctccc cggcttctag agagctgcca gaagcgcgtc
     2701 gtcggtgggg gtgtcgccaa cacgcaggtg cgagccgatg accgcgggga gtggcggctc
     2761 caggcatgtc gagcgcagcg catcccacga ggcctcggca aagaccgatg ccggcccgcc
     2821 gtccaggatc accctgccct ctcggagcac gaccacccgg tcgaaggacg cggccacgaa
     2881 ttccatgtcg tggctgatgg cgatgaccgt cctgcccgcg gcgtgagctg cagcgatgac
     2941 ccgctttacg atctccaccc cccgcatgtc ctgtccggtg gtcggctcgt cgaggaccag
     3001 caccggggtg cccatggcct ggaccgatgc cagcgccaac agcttgcggc gcgacgcgcc
     3061 aaggtcgtac gggttgctgt cggcctcctc gccgaggccg accacggcga gggccgtgtc
     3121 gaccgcggtg cgcaattcgc cgccgctcat gcccaggttg cgcggcccga actccacctc
     3181 gcgacggacg ctgccggcaa agatctggcg atcgggatcc tggaaggcga gcccaacagc
     3241 gcgtgccagc tgcgcgacgg tctgccgggc cacgtccctg ccgtcgtgca ggacgcgacc
     3301 cgatgtcggt cgcagcaacc cgttcaggtg ccgcaccagg gtcgtcttgc cgctcccgtt
     3361 ctgaccgatg agagccacgc gttcgccggt gccgatgtgc agctcgatcc catcgagggc
     3421 cagcgtgccc ccgtgcgggt agcggtaggt gactgcctcg agggtgagat tggtcatcgg
     3481 ggcgggacct ccgcgatcgc atcggcgagg cgcgcgaggg acgctgccgg caggttcgct
     3541 tccgcggccg ctcgccgcag gcggaccgcc gccgggggcg ccaccccgag gtcggtcagg
     3601 cgcggatcgg ccaggacctc gaccgcgggt ccgtcgagca ccacccgccc ggcgtcgatg
     3661 gccgcgaccc gcgaacagat ccccgccagc agatcggtct tctgctccgc gaccaggatc
     3721 gacgccccgt tggctgcgag gcccgccagc gccgccgcga ccatcgccgt cccggccggg
     3781 tcgagctggg ctgtgggctc gtcgaggacc aggtgcgccg ggcgaagggc gagcaggccg
     3841 gcaatcgcga cgagctgctg ctgaccgccg gagagctggg cagggtcgcg atcggcgagc
     3901 gactcgatcc gcagggcgct gagggcctcc tcggttcggg cgatcagctc cgaacggggg
     3961 acacccaggt tcatcggtcc gaaggccacc tcctcgtaga ccgtggcggc caccccggaa
     4021 agctgcgtgg ccgggctggc gaaggcgatc ccgatccgcc ccgccaggtc gtgcatcgcc
     4081 agcggcgccg tgtcctcccc gtcgagcagg aggcggccgc tgagcgtccc gcccacggtt
     4141 cgtggcgcga gcccggacgc gaccaggcag atcgtcgtct ttccggcctc cgaggcgccg
     4201 accaggccga gcacctcgcc ctcgcgcagc tcgaggctca cctcgtggag ggaggggcgc
     4261 agcgccccgg cgtagcggta ggtcagggat tcgaggacca gcgtcagcgc agccacccca
     4321 gccagccgag cgcgcgtgcc acgatcagca gcgggaccgc caggacgagc agccagcggg
     4381 ccaccctttg ccgcgctgag tccgctggcc accacagcag cgtccggcgc ccggggtggg
     4441 tgaagccgcg tgcctcgagg gccatggttc gctcctcgac ctcggtgatc gagccgagca
     4501 ggaccggtcc ggcgaggggc aggactcccc gtagccggcg ccagacggat ccctcggtgt
     4561 ccagaccgcg cgcccgctgc gcggccgtga tggtcgccgc tcgctcgacc agggccggca
     4621 ccgtctgcac cgatgctagc gccacgaacg caatccgcgg cgagacgcct cgccgctcga
     4681 ggtcgagcac cagctccgat gggggagtgg tcaggtagaa gagggtgatg gcaccgctga
     4741 tggcgccgat gcgggccagt gtctcgagcg cgaacccgag gccctcggcg gtggccgtga
     4801 tcggtccgac ctggaacagg atctcgcgcc cggcggggaa gaagaacagg ttgaccagca
     4861 gcacgctgat cgcgatcggc agggacagca gcagcgccgt gcgcagcaga cggccaagga
     4921 tccggccgat gagcgcgggc agcacgaccg ccaccaccgc caggatggcc ggaccaagca
     4981 gcccgccgga gagcacggcc agcacggtgg tcaccgccgc cagtgccgcc ttggtgagcg
     5041 ggttgaggcg atggacgctg gtcggtccgg gcgcccccag gaacgagggg aggcgatcgg
     5101 tcaggccgaa ggctcctcga gcacctgctc cccctgcggg aagcgcgcct tgaagcggtt
     5161 cgccagcgcc gagaggatca ggtatacggt gaagaaggtc gtgaccttgt cgatcgggtc
     5221 actgatgagc ccctgccccg ttgttgcggc gtagacgtcg gcgccggccc ggcgaaaggc
     5281 agccaccagg aagtcggtgc cggaggcggt gacccctccg aagacgccgg cagcgatcgg
     5341 tgcgctgatc agggccgcca cgatcccggt gaagactccg gccaccacca cgtaggccgc
     5401 cgtcaggtcg cgacgcacta ggaggagcgc gaagagaccg accaccgcca gcgcgacgag
     5461 ggccacggcc aggtagccaa ggagcacgaa gatgagatcg gtcttggcgg ggtcggggaa
     5521 caggtcgatc ttggtctcga agatcctggt gtaggccagc gccgccgccc aggtgagccc
     5581 cccgaccagc acaaccgtca ctgcaccacc ggcgaggagc tccgcgggcg ggcgattcgg
     5641 ccgcgggcgc aggaatccgg cacgccccag caagccggcg atcacgccga tcgccaccgc
     5701 gacgatggcg aacgccgcag ccggctggta ctggaacgga ggcgggatga cgtacgacca
     5761 caggatgttg ccgaggccgc cggtgagcgc cccggcgacg ggcccgcaca gcaccccgac
     5821 caggatggtc ccgatcgaat cgaggtagat cgggatcttg agggccgatg ccacggtctg
     5881 gccgaggatc acgttgatcc cgattccgac cgccatgagc aggtaggttc gggtgccgaa
     5941 ctgccttgat agcgacgcca ggcgcccggt ctgcgccagc tccaggccgt aggtgacaac
     6001 ggcgccgatg accatggcca cggtcaggat caaggcccag gagttcgcgg ccttggtgtt
     6061 ggcgaaggtg ttgggatcta ccgcgttggc gtcgccgaag tcgccgaagc caatgatccc
     6121 gatgtaggtg gccgccgcga acgcgacgag gcctgcggcc actcgcatcg gcccccattc
     6181 gcctgtctgc gccaggctca ggcccgcgta cacgacgtcg gccacgatga cgccgaccgc
     6241 cgcggagagc ggccacacct gccccgtggc atacaggccc aggccgacga gtgccccgac
     6301 caccaccccg attgcgatgc gcgtccagcg agcggaatcc atgtcctcaa cctccctcgt
     6361 gcaatgcctg cacctgcgcc gtcgtcaccg agaccgcgtg cgcgaggcgt tccagcagtt
     6421 cccgatgcat cccctcggcg tccagggccg tcgcgacgtt cacggctcgc cgattctcgt
     6481 cgcgggatcg gtacaggctg ccggccaggt gcggattgct gccggtgtcg cacgccagcc
     6541 gctcgtcgac ggagtcggtg acgaggtcgg ggcgcagcaa tgagccgatc agcgtcaccg
     6601 ggtcatgcag gtagcagccg tcgatcccct ctgcctcccg gtggaagctg atgtagaaac
     6661 gcaccgcatc ccggacgatc gccgacagcg gcgtgcccgg caggccgtcg agatcctcca
     6721 gggtgaagat gacatcctgg gtcgcgtcga gcggaaagat gcgagagatg gcgccagccg
     6781 ccaggaccgc ctcggcggcc tcgggatcga cgcacgcgtt ccactcgccg gtctcggtcg
     6841 tgttgccccg ctcctccagg gcgccgccca tccagatcag ctcccggagc ccgcccgcca
     6901 ggtccacgcc gttcagcgcg gcggaggcaa tgttggtgag cggacccagc gccacaacgg
     6961 tcagctggcc cggacggcgc tggatatgcg ccgccaggta gcgcggcgcg gggaccgccg
     7021 tgtagtcggt cgcgccgaga tcgggatcct cgccggtcag ctcgacgtag ccggtgccgg
     7081 tcggcccgtg ggtatccggc gcggtccgca ggcgccgatg caggggccgc gcggcgccga
     7141 tgctgaccgg cacgtcgccc ctcccgacca tgcgcaggat cttgccggtg ttgcgtgcca
     7201 cgtggtggat ctcgacgttg ccggcaacgc aggtcacgcc gcgcaggttg agctcgggtg
     7261 cggtggccgc gatgagcaat gcgatcatgt cgtcgatccc ggtgtcgacg tccaggatga
     7321 ccgggcgggg ctcggcgtcg gtcgggctcg cctgcattga cgtcatcgta ccccgctatt
     7381 gcggccaggc accggcacga cccaccttgc tgccgacctc cgtgccgagc tcgccggcga
     7441 tgaaccgtgc ggcggccagg atgccgtcga ggttgacgcc ggtccgcacg ccttcccggt
     7501 cgagcaggta caccaggtcc tcggtcgcca ggttgcccgc ggagccaggc gcgtacggcg
     7561 atccgccggt cccgccggcc gatgaatcga agcatcgcac tcccagttcg atggccgcca
     7621 tgacgttggc gagggccgtg ccacgggtgt catggaggtg catggcgacc acctccgggc
     7681 cgatcccccc cgcgcccagg gccgccagca cccgcgccac gtcaacggga gccgccacgc
     7741 cgagcgtatc ggagatcgac agctcgtcga cccccaggcg caacagccgt tccgcgacgc
     7801 tgacgaccgc tcgctcgtcc acctcgccct cgtacgggca gccgaacgcg gtgctgacat
     7861 aggcgcggat ccaccagccg cgacggcgcg cctcggcggc gatcggcccg aacgcggcca
     7921 gcgactcgtc gacgctcatc ccgatgttgt gctgaacata ggagtccgtg gctgcggtca
     7981 tgaggcagat ggcgtccacc tccgcactga tggcccgctc caggccgcga gcgttcggga
     8041 cgaggaccgg gtaccgaacg cccgggtgcc gctccagccg tgtcaggagc tcgtccgcat
     8101 ccgccatctg cggaatcgcc ttcggcgaga cgagcgaggt ggtctcgatc tcgcgcaggc
     8161 cggcatccgc gagcatggcg acgaaggcca gcttcgcatc ggtcgacacg acggtcgcct
     8221 cggcctgcag cccgtcgcgc gggccaacct cgtagatgcg cacctcgggc cgggtcatcg
     8281 gtcggccatc ttaggccgat acctccgcca gcaggtcccc gcgctgcacc tgctggccaa
     8341 ccttgaccgc gacgtgcgtc accgtcccgg tgagtggcgt gacgacggcg tgctccatct
     8401 tcatcgcctc gatcacgacg agcgc
//
LOCUS       DATCQR010000049         2065 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_157281, whole genome shotgun sequence.
ACCESSION   DATCQR010000049 DATCQR010000000
VERSION     DATCQR010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157281"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..444)
                     /locus_tag="VIN32_01680"
     CDS             complement(<1..444)
                     /locus_tag="VIN32_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921315.1"
                     /translation="MADAGPRGGGERGRADMHLHTLYSDGVMEVQALLDHVERSTELD
                     LIAITDHERIDGALRAQELHAAGDYHFGLVVGEEITTRRGHLLALFLTERIPALRPLE
                     ETIERIHAQGGIAIAPHPMAPLTPSLGRRSLMRLHQDPDLAHRLDG"
     gene            complement(437..1330)
                     /locus_tag="VIN32_01685"
     CDS             complement(437..1330)
                     /locus_tag="VIN32_01685"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921316.1"
                     /translation="MGGVLLEVGLRAADLVASLLPRGAAYALADLIGWSWYRLAARRR
                     TLVAANLARVCAAAGRPTNGRAFRRLVRQAFVEHARYYLELLRIPHVSIEQVGAMVSV
                     EEWDRWKALLGKGAVVATMHLGNPEPYGSLIAASGLHAVVPVEEIRPKALYEFLFARR
                     ASGRGVTMMPLSKARRALMEELRRGGIAGVVADRNIAGRGHPTTFFGARASLPTGPAS
                     LALMSGRPLMAAACWRIAPERFNARAWLIEAERTGDRRVDVAALTEAMARRMEEAIAV
                     SPEQWFAAFQPIWDEEGSGGG"
     gene            complement(1330..>2065)
                     /locus_tag="VIN32_01690"
     CDS             complement(1330..>2065)
                     /locus_tag="VIN32_01690"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEY8921317.1"
                     /translation="RRAFRNYAKYLVDVMRLSTLTADEVAKLVAIDNLEILDEARAGQ
                     RGVLLCTVHVGGMDLIAPALHRFGHEMHVVADDTTYGRLYEHLRAIRARHGLTLIGWR
                     SLRGLFKALKAGSNLVLFCDGGYRDGDVPVDFLGEPTTFPAGPAALSARSGAPMLPVA
                     CSRLRNDRFRTRGLPLITAANDSPAETYRATQALADALGSVIAEDPGQWYMFRPVWPQ
                     TDADRARARAALDAARRGEDWTKPSP"
BASE COUNT          303 a          731 c          743 g          288 t
ORIGIN      
        1 gccgtccagc cgatgggcga ggtccgggtc ctggtgcagg cgcatcaggc tgcggcgccc
       61 gaggctgggc gtgagcggcg ccatcgggtg cggggcgatg gcgatcccgc cctgggcgtg
      121 gatccgctcg atcgtctcct ccaggggacg aagggcaggg atccgctcgg tcaggaagag
      181 cgccagcagg tggccgcgcc gggtcgtgat ctcctcgccc accaccaggc cgaagtgata
      241 gtcgcccgcc gcatgcagct cctgggcgcg gagcgcgccg tcgatccgct cgtgatcggt
      301 gatggcgatc aggtcgagct cggtggaccg ctcgacgtgg tcgagcagcg cctgcacctc
      361 catcacgccg tccgagtaga gggtgtgcag gtgcatgtcc gcgcggcccc gctcgccgcc
      421 gcccctcggc cctgcgtcag ccaccgccgg aaccttcctc gtcccagatc ggctggaagg
      481 ccgcgaacca ctgctcgggc gagacggcga tggcctcctc catgcgccga gccatcgcct
      541 cggtcagggc ggcgacgtct acgcggcggt cgccggtgcg ctcagcttcg atgagccacg
      601 cgcgggcgtt gaaccgctcc ggcgcaatgc gccagcaggc cgcagccatc agcggacggc
      661 ccgacatgag ggccaggcta gcgggaccgg tgggcaggct ggcccgtgca ccgaagaagg
      721 tggtcgggtg ccccctgccc gcgatattac gatccgcgac cacccccgcg atgccgcccc
      781 tgcgcaactc ctccatcaac gcgcgtcgag ccttcgatag cggcatcatc gtgaccccgc
      841 gcccgctcgc gcgccgcgcg aagaggaact cgtagagcgc ctttggtcgg atctcctcca
      901 ccggcacgac cgcatgcagg ccgctggcgg cgatcagcga gccgtatggc tcggggttcc
      961 cgaggtgcat ggtcgcaacc accgctccct tcccgaggag ggccttccag cgatcccact
     1021 cctcgacgct gaccatcgct cccacctgct cgatcgagac gtgcgggatg cgcagcagct
     1081 cgaggtagta gcgggcgtgc tcgacgaacg cctggcgcac caggcgccgg aaggcccgac
     1141 cgttggtcgg ccggccggcc gccgcgcaga cgcgggccag gttggcggcg accagcgtgc
     1201 ggcgcctcgc ggcgagccgg taccagctcc agccgatgag gtcggccagc gcgtatgcag
     1261 cgcctcgcgg caggaggctt gcaacaagat cggcggcgcg caggccgacc tccagcagca
     1321 cgcctcccat cagggcgacg gcttcgtcca gtcctcgcca cggcgcgccg catcgagggc
     1381 agcgcgcgcc cgcgcgcgat cggcatcggt ctgtggccag acagggcgga acatgtacca
     1441 ctggccgggg tcctctgcga tcacgctgcc cagcgcgtcg gcgagtgcct gggtggcgcg
     1501 ataggtctcg gccggtgagt cgttggcggc ggtgatgagc ggaagaccac gggtcctgaa
     1561 tcggtcgttc cgcagccggc tgcaggcgac cggcagcatc ggcgcccccg agcgagccga
     1621 aagggcggca ggaccggccg ggaaggtggt cggctcgccc aggaagtcga ccggcacgtc
     1681 gccgtctcga tacccgccgt cgcagaagag gaccaggttc gatcccgcct tgagggcctt
     1741 gaagagcccg cgcaggctgc gccatccgat cagggtcagg ccgtggcggg cgcgaatcgc
     1801 gcgcaggtgc tcgtacaggc gcccgtaggt cgtgtcgtcg gcgacgacgt gcatctcgtg
     1861 cccgaaccgg tgcagcgccg gcgcgatgag gtccatccca ccgacgtgga cggtgcatag
     1921 cagcacgccg cgctggcccg cgcgagcctc gtccaggatc tcgaggttgt cgatcgcgac
     1981 cagctttgcg acctcgtcgg ctgtcagcgt gctcagccgc atcacgtcga cgaggtactt
     2041 ggcgtagttg cggaaggcgc ggcga
//
LOCUS       DATCQR010000050         4692 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_141911, whole genome shotgun sequence.
ACCESSION   DATCQR010000050 DATCQR010000000
VERSION     DATCQR010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4692
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141911"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..774
                     /locus_tag="VIN32_01695"
     CDS             <1..774
                     /locus_tag="VIN32_01695"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEY8921318.1"
                     /translation="EVQRTRRSGRGFCVLMIDLDGLKAINDSLGHLRGDDVLRAVGRV
                     INGSIRTVDSAYRYGGDEFLILLPETEFIGAYVVAEKIREGVEEIGESLATDLAATSV
                     SIGLVSHPEDGSSPEELMVAADRAMYQAKALGKNQISGNPRPRRAPQRLLGSPDPVSD
                     PAPADASPLEARPAAPAAVAADPPPRSGATAFSVEPAKTNGSQHDAHGEDEPDPADVR
                     RNIAAARRNMDPDHQIRRAMDAFLSSPTSSRADRERGPS"
     gene            complement(771..1904)
                     /locus_tag="VIN32_01700"
     CDS             complement(771..1904)
                     /locus_tag="VIN32_01700"
                     /inference="COORDINATES: protein motif:HMM:NF022702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HEY8921319.1"
                     /translation="MQFLRGLSRRQQVAGAAAAVVVLGVAIWLGLGLGSRPEPQPSPS
                     ESPSPTASPSPSLVPTPSATPELATCPLSGLPLEDPSIAERVAIAVQVENHPLARPAR
                     NLSNADMVVEAPVEGDVTRFSAIFLCRPTDGLTGPIRSARYYEVDLWQDLGVLPVGYG
                     ASFGARSKFTAAGMPYVNGITGQWPWFQRAGGNAAPHNLYGDLDSLRAAIGQGGAIDR
                     LARLVHTLRPPFTFEVGVALPAGRPVSAFEIRTNSYWRFGWTFNAAEGGWTRQDAGKT
                     VVDGVNNRPLSATSVVVQRVEEEVVFGDPDPGGNSRRLQHLVGKGDGTLYSQGKAIAL
                     HWSRPTAADGTEWTYADSGEPVVLPPGQVWWEIIPIRATLTES"
     gene            1955..3325
                     /locus_tag="VIN32_01705"
     CDS             1955..3325
                     /locus_tag="VIN32_01705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEY8921320.1"
                     /translation="MDDAPEQRYGPPPSGPRASLAAFLSFLFPGLGQAYNGEAGLALL
                     LAAPVALLVGLVGLVLATASSQVLARLFDVRFLIGLIVMDLLLLGWRLIAVLQAHGHW
                     ARPSMRSRATWATALLVTVTLAMHAVPAFYAVKAIDTLGSVALGGTHGPSLNNTLPAF
                     GRLGEPSGQPDVGAGERVNVLLVGIDSAPGRTTQLTDTMLVVSLDPNGSRSAMISVPR
                     DLYGVPLPDGTPYNQKLNSLMTFAADHAADFPLGGVGTLKATIGKLLGLPIHYFAAIN
                     LLGFQQAVDSIGGVDMTVTRAVSDPTYVDEYGHRIGFYIQPGAYHMDGRTALAFVRSR
                     KGVGDSDFTRADRQQQLLTAIRDKLTAGNMLLALPGLLDAVKNTIATDVPSGRLAELA
                     QAVQGADVSHLQRVVLQPPDYMRADPFSSAGYILVPDLEAIHAIGEQLLSGGTIGATP
                     EPSASP"
     gene            3350..4534
                     /locus_tag="VIN32_01710"
     CDS             3350..4534
                     /locus_tag="VIN32_01710"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY8921321.1"
                     /translation="MPGFATRAIKAASRVPAAPQPPVNVPIYQTSTFEVGSAVELAEL
                     LEFARPGHSYSRYSNPTHAALEGALAELEGAEACLATASGMAAIHGLVMSFLRTGDEL
                     IIPRAVYGGVVGLSQTVLARAGIEVTAVDTTDRTSVAAAMGDRTRLVWLETISNPTTA
                     MADIAVISELAHAVGAAVAVDNTFASPYLCNPLALGADAVVHSTTKYIGGHSDLTGGA
                     ILGSIEVVGAAREIVINAGGNAAPLEAFLALRGLKTLALRMGRHSANALAAAEALEGA
                     PGVDAVHYPGLASHPQHAMSRRTLRDGMAGGMLALELLGGRTGGERFLDLLQVAVHAT
                     SLGSVETLCSHPASSSHRQLSDAELAEAGLSPGMIRVSIGLEDPEDLIADLANAAVGA
                     AT"
BASE COUNT          730 a         1654 c         1558 g          750 t
ORIGIN      
        1 gaggtgcagc gcacccgccg cagtggccgc ggcttctgcg tgctgatgat cgacctcgac
       61 gggctgaagg cgatcaacga ctcgctgggt catctgcgcg gcgacgacgt gctgcgcgcg
      121 gtcgggcgcg tcatcaatgg atcgatccgc accgttgatt ccgcctaccg ctacggcggt
      181 gacgagttcc tgatcctgct gcctgagacc gaattcatcg gcgcctatgt ggtcgccgag
      241 aagatccggg agggggtcga ggagatcggc gagtccctgg cgaccgacct cgcggcgacc
      301 agcgtgagca tcggcctcgt ctcgcatccc gaggacgggt cgtcgcccga ggagctgatg
      361 gtcgccgccg accgggccat gtaccaggcc aaggcgctcg gcaagaacca gatcagcggt
      421 aatccacgcc cgcgccgggc ccctcagaga ttgctgggat ctcccgaccc ggtcagcgac
      481 cctgcgccgg ccgatgcctc cccgctcgaa gcccgccctg ccgctccggc cgctgtcgcc
      541 gccgatcctc cgccgcgctc aggtgcgaca gcattcagcg tcgagccggc gaaaaccaat
      601 ggttcgcagc acgatgcgca tggcgaggac gagcccgacc ccgctgatgt tcgacggaac
      661 atcgccgcgg cgcgccgcaa catggatccg gaccaccaga tccgacgagc gatggatgcg
      721 ttcctctcca gtccgacctc gtcacgtgcc gatcgggagc ggggaccctc ctaggattcg
      781 gtcagggtcg cgcgaatcgg aatgatctcc caccagacct ggccaggggg aagaacaacc
      841 ggctcaccgc tgtcggcata ggtccattcc gtcccatcgg ccgcggttgg gcgggaccaa
      901 tggagcgcga tggcctttcc ctgcgagtag agggtcccgt cgcccttccc cacgaggtgc
      961 tggaggcgcc tcgagttgcc gcccgggtcg gggtcgccga acacgacctc ctcctccacg
     1021 cgctggacga ccacgctcgt ggcggacagc ggcctgttgt tcaccccgtc gacgaccgtc
     1081 ttgcctgcgt cctggcgggt ccatcctccc tccgccgcgt tgaacgtcca gccgaaccgc
     1141 cagtagctgt tggttcggat ctcgaaggcg cttacggggc gcccggcggg gagcgcaaca
     1201 ccgacctcga aggtgaacgg cgggcgcaac gtgtggacga ggcgggcgag gcgatcgatc
     1261 gcccctccct gtccgatagc agccctcagg ctgtccaggt cgccgtacag gttgtgcggg
     1321 gcggcattgc cgcctgcccg ctggaaccag ggccactggc cggtgatgcc attcacgtac
     1381 ggcatgccgg ccgccgtgaa ctttgagcgc gcgccaaagc tggcgccata tccaacgggc
     1441 aggacgccga ggtcctgcca caggtcgacc tcgtagtagc gggcgctgcg aatcggtccg
     1501 gtcaggccgt ccgtcggacg gcacaggaag atcgcgctga accgggtgac gtcgccttcc
     1561 accggtgcct ccaccaccat gtccgcgttc gacaggtttc gcgccggcct cgcgagggga
     1621 tggttctcaa cctgcaccgc gatcgcgact cgctcagcga tggacgggtc ctcgagtggc
     1681 agtccgctca aggggcaggt ggccagctcg ggggtggcag acggggtggg gacgaggctc
     1741 ggcgacggag aggccgtcgg ggagggactc tccgagggcg acggctgcgg ttcgggtctc
     1801 gaccccagtc ccaggccaag ccagatggcc acacccagga cgaccaccgc tgccgctgct
     1861 ccagccacct gctgccggcg gctcaaacca cgcaggaact gcatctccaa atcctaaccg
     1921 gtcgccccga cctcagccgt ggcaccattg tcatgtggac gacgctcctg aacagcgcta
     1981 cggcccgccg ccctcagggc cgcgggcttc gctcgccgcg ttcctctcgt tcctcttccc
     2041 gggcctcggg caggcgtaca acggggaggc gggactggca ttgcttctcg ccgcgcccgt
     2101 ggcgctcctg gtagggcttg tcggactggt gctcgccaca gcctcctccc aggtcctcgc
     2161 ccgcctcttt gacgtgcggt tcctgatcgg cctgatcgtg atggacctgc tgctcctggg
     2221 ctggcggctg atcgcggtcc tgcaggccca tggtcattgg gctcgtccca gcatgcgcag
     2281 tcgagcgacc tgggcaacgg ccctgctggt gacggtcacg ctggcgatgc atgccgtgcc
     2341 cgcgttttac gcggtcaagg ccatcgatac ccttggcagc gttgccctgg gtggcacgca
     2401 tggcccgtcg ctgaacaata cgctccccgc cttcgggcgg ctgggggagc catccgggca
     2461 gccggacgtc ggcgcgggcg agcgcgtcaa cgtcctgctg gtggggatcg acagcgctcc
     2521 cgggcgaacc acccagctga ccgacacgat gctggttgtc agcctcgacc caaacggatc
     2581 gaggtccgcg atgatctccg tgccgcgaga cctgtatggg gtccccctgc cggacggcac
     2641 cccctacaac cagaagctga actcgctcat gacattcgcg gcagatcatg ccgctgactt
     2701 ccctctcggt ggcgtgggca cgctcaaggc gaccatcggc aagctgctcg gactgccgat
     2761 ccactatttc gcggccatca atctcctcgg cttccagcag gccgtcgact caattggcgg
     2821 ggtggacatg accgtcacac gcgcggtcag cgaccccacc tacgtggacg agtacggtca
     2881 ccggatcggc ttctacatcc agcccggcgc gtaccacatg gatgggcgta ctgccctcgc
     2941 cttcgtgcgc tcaaggaaag gcgtcggcga cagcgacttc acaagggccg atcgccagca
     3001 gcagctgctc accgcgattc gcgacaagct gaccgccggg aacatgttgc tggcgctccc
     3061 gggattgctc gacgcggtca agaacacgat cgccacggac gtgccgtcgg gacggctggc
     3121 ggagctcgcc caggccgtcc agggagcaga cgtgtcgcac cttcagcggg tcgtgctcca
     3181 gccgccggac tacatgcgcg ctgacccctt ctcgagcgcc ggctacatcc tggtcccgga
     3241 cctggaagcg atccacgcta tcggcgaaca gctgctgagt ggcggcacga tcggcgcgac
     3301 gccggaaccg tccgcctccc catagccgac cgctagactc ggatcggtca tgcccggctt
     3361 cgccacccgc gccatcaagg cagccagccg cgttcccgct gccccccagc ctccggtcaa
     3421 cgttccgatc taccagacca gcacgttcga ggtcggcagc gcggtggagc tggccgagct
     3481 gctggagttc gccaggccgg gccattcgta cagccgctat tcgaacccta cccacgctgc
     3541 cctggagggg gcgctggccg agctggaagg tgcggaggcg tgtctcgcca ccgcttccgg
     3601 gatggccgcc atccacgggt tggtcatgtc gttcctgcgc acgggggatg agctgatcat
     3661 tccgcgggcg gtctacggcg gggtggtcgg cctctcgcag acggtccttg cgcgagctgg
     3721 catcgaggtg accgcggttg acaccacgga tcgaaccagc gtcgccgcgg cgatgggaga
     3781 tcgcacgcgc ctcgtgtggc tcgagacgat cagcaatccc accaccgcga tggccgacat
     3841 cgccgtcatc agcgagctgg cccatgcggt cggtgcggca gtcgccgtcg acaacacctt
     3901 cgcttcccca tacctgtgca atccgctcgc cttgggcgcc gatgccgtag tccactccac
     3961 caccaagtac atcggcgggc actcggacct gaccggtggg gcgatcctgg gcagcatcga
     4021 ggtggtcggt gcggcccgcg agatcgtgat caacgccggt ggtaacgcgg cgccgctcga
     4081 ggcgttcctg gctctgcgag gcctcaagac gctcgcattg cgcatgggcc gtcacagtgc
     4141 caatgccctc gctgcggccg aggcgctgga aggggcgccc ggggtcgacg cggtgcacta
     4201 tccaggcctg gcatcgcatc cgcaacacgc gatgtcccgg cggacgctgc gcgacggcat
     4261 ggcaggcggc atgctggcgc tcgaactgct gggaggacga accggcggcg aacggttcct
     4321 cgacctgctg caggtcgccg ttcacgccac cagcctgggc tccgtcgaaa ccctgtgcag
     4381 ccatcccgca tcctcctccc atcgccagct gagcgacgcc gagctcgccg aggcggggtt
     4441 gagcccgggg atgatccggg tctccatcgg cctcgaggat ccggaggacc tcatcgcaga
     4501 cctggcaaac gctgccgtgg gcgcagccac ctagcacgcg gaggacccga tgagccgatc
     4561 gaccgagctg gccgccagcg gcggcatggc gatcgccctc tggctcccgc gacggcggat
     4621 gatgctgcgg actgcggtcg cgtgacgagt ctgctcgacc tgtggcgcgc catcgacccc
     4681 gaggcgcggc tc
//
LOCUS       DATCQR010000051         5304 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_48945, whole genome shotgun sequence.
ACCESSION   DATCQR010000051 DATCQR010000000
VERSION     DATCQR010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5304
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48945"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            517..684
                     /locus_tag="VIN32_01715"
     CDS             517..684
                     /locus_tag="VIN32_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921322.1"
                     /translation="MRRRGLVSNMYRAARLANDMSVLASGNPHRIARGARNKIVGRAL
                     ARGGFWRLLWG"
     gene            1099..2304
                     /gene="chrA"
                     /locus_tag="VIN32_01720"
     CDS             1099..2304
                     /gene="chrA"
                     /locus_tag="VIN32_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015002331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HEY8921323.1"
                     /translation="MPSRPGSDVAPHADEATIGALCRYFLRLGALGFGGPVALAGYMQ
                     RDLVDERGWISPQEYRDGLAIAQMAPGPLAAQLAMWLAFIRTGVRGATLATFAFVGPP
                     FLLVVGIGWLYVGLGGAHWVGSLFYGIAPAAIAIIALAAYRLLPLTIGRDPLLFVVAV
                     GVGLLTFFTQSEVALAFLAAGFLVVLVRVGPRALARAVGGRMAGAAILGPLLPAGAID
                     PGLLGGLALFFIKAGAFVFGSGLAIVPFLHQGVVVEHAWLTERQFLDAVAVGLITPGP
                     VVITAAFIGFIVAGLPGALVGAFGVFLPAYLFVIVPGRWILRHKDAVPVRAFVAGVSA
                     AAAGAILAASVILGRGAIRDGATLAIGLLALAALLAIRRQQLRYIERLAEPLVVVVAG
                     IAGLLLRGA"
     gene            complement(2423..3082)
                     /locus_tag="VIN32_01725"
     CDS             complement(2423..3082)
                     /locus_tag="VIN32_01725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921324.1"
                     /translation="MQDGTAGFHEHNRVMSNGHDALRHAESIAGEALGPAPPVPPFLF
                     LEQTPPDPLAEAKTFGQPAARLTVGGNDLARPLLAMDQHAHNQTRAFEGRPALARMAD
                     DRFDYLARWRGRPRHRPPAVADVIAAREGCEFEGVRGAADVLDQRQIVDIRKIGWPKA
                     KPAAERNTECRDAERQLHRLAHSEVCGQRQRRDQFGQSEPSGGHRPRLGYRLAHPRIF
                     R"
     gene            3215..4006
                     /locus_tag="VIN32_01730"
     CDS             3215..4006
                     /locus_tag="VIN32_01730"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEY8921325.1"
                     /translation="MTVPGAAIDRVALALADFTDLKSPYLLGHSTRVAEVASGAADVL
                     ACAPAEVDEVRRAALLHDLGRVGVANGIWDKRGPLTESERERVRLHPYYTERILARSP
                     ALASLAVIAGSHHENLDGSGYHRGVGATQLGRPARLVRAADCLDAMASDRPHRVALPD
                     DGRIRELRAEVDAGRLDAEAVAAVIEASGGGRVHLRPPRPAGLSEREIEVLRLLVHGI
                     SNAEIGTRLRLSPKTVGHHVEHIYNKAGVTSRAAAALFAMETGLI"
     gene            4166..4894
                     /locus_tag="VIN32_01735"
     CDS             4166..4894
                     /locus_tag="VIN32_01735"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY8921326.1"
                     /translation="MLLIPGATGDAGTFTTAATRLADEFTVITYDRRGNSRSRATGDP
                     MAAATMSAQADDAADLIVACGFNKAVVYGNSGGAIIALELLARRPTMVKGAVVHEPPL
                     IALLPQDGPNLLEPIFQLAQTDPRAALEAFVRVNTSDAAWEAIDSGTRERILGNGETL
                     FAREMGQFISYQPDEAVLRAQRVPVVLLRSRDGLEFAPHVLAWLQDRLGVEVGTLSGQ
                     HSPFLDIPEVFAEELRPILRRLWN"
     gene            complement(5056..>5304)
                     /locus_tag="VIN32_01740"
     CDS             complement(5056..>5304)
                     /locus_tag="VIN32_01740"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF002017.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HEY8921327.1"
                     /translation="LPLWRLADGRLMRTVKTRDFRSALELVTRIADPADEQNHHPDVA
                     IHWNVVMLTLWTHGSGGITERDFRLADAIERTLSSPAG"
BASE COUNT          745 a         1800 c         1844 g          915 t
ORIGIN      
        1 acagccaggc tcagtccccg atggggtcgc tcctcgacgt agtgagcgac gtacgcttgg
       61 aggactcgct cgaggtgccg tcggctgtag atcaggacgt gatcgaggca ctctcggcgc
      121 acggtcctga aggaaccgct cggcgaatgc attcgcccga ggtgcgcgga gcggcgatcg
      181 gatgacccgg acgccctcgc cgcggagcac cgcatcgaat ggtcccgaga acttggcgtc
      241 ccggtcgcga acgaggaacc gaactcccga cagccgctcc tcgatggcga ggttcctggc
      301 ttgctgggtg acccacgccg agtcggggtt ggcggtgacg ccggcgacgt gcacccgccg
      361 ggtcgcgagc tcgatgaaga agaaggacgt acatcgtctt gagcgtgatc gtctcgacgg
      421 tgaagaagtc ggtcgcgagg atccccgccg cctgtgaccg gaggaactcg gtccacgttg
      481 ggccggcccg gcggggagca gggtgggagc gcgaccgtga gacgacgggg cctggtctcc
      541 aacatgtacc gagccgcacg actggcgaac gacatgtccg tcctcgcctc gggcaacccg
      601 catcggatcg cccgcggggc gcgcaacaag atcgtcggcc gggcgctggc ccggggtggc
      661 ttctggcgcc tcctctgggg ctgagcggtg aacggtgagc ggcgccctat ccattgaggc
      721 tggatcggct tctacctggt cctggtcgtc gccggcatga cgctctcgct cgcgggcggg
      781 taccggggcg atctcttctg gctggcgatc ggctggatga tcgccctggg cgtcatcacc
      841 cggatccttg gcctgcggcc gccgcgctga gcccgaaacg agatcaaggc cccgatcggc
      901 cctcgataat ccggcagagg cttcctcgcg tcgatccaca ctcccaatgg aacgtgcatt
      961 gttgacatca aggtcgccga ttcgtgaagc gccagccgtc aaaggcgagg cgcactcgtt
     1021 tgtcctagcc gccgatcggg agatcgcggt caccgtcggc gctcggtgcc aggctgccaa
     1081 gtgctagccc ggcctcgaat gccttcccgc ccgggctctg acgtggcgcc gcacgccgac
     1141 gaggcgacga tcggcgcgct ctgccgctac ttcctgcgtc tgggtgccct cggcttcggt
     1201 gggcctgtcg cactcgccgg gtacatgcag cgcgacctcg tcgacgagcg cggctggatc
     1261 agcccgcagg agtaccgcga cggcctcgcg atcgcccaga tggcgccggg tcccctcgcg
     1321 gcgcagctcg cgatgtggct tgccttcatt cggaccggcg tccgcggggc gaccctggcc
     1381 acgttcgcct tcgtggggcc gcccttcctc ctggtcgtag gcatcggctg gctgtacgtc
     1441 ggcctcgggg gggcgcattg ggtcggctcg ctcttctatg gcattgcccc ggccgcgatc
     1501 gcgatcatcg ccctcgccgc ctaccggttg ctccccctga cgatcgggcg cgatccgctc
     1561 ctcttcgtgg tcgccgtcgg ggttggtctc ctcaccttct tcacccagag cgaggttgcg
     1621 ctcgcgtttc tcgccgccgg ctttctggtc gtcctcgttc gagttgggcc gcgggcgctc
     1681 gcgcgcgccg tcggcggtcg aatggccggt gctgcgatct tgggcccgct gctcccggcc
     1741 ggtgcgatcg acccggggct gcttggcgga ctcgcgctct ttttcatcaa ggccggcgcg
     1801 ttcgtcttcg gctcgggtct cgcgatcgtc ccgtttctcc atcagggtgt ggtcgtcgag
     1861 cacgcctggc tgaccgagcg acagttcctc gatgcggtcg cggtcgggct gatcaccccg
     1921 gggccggttg ttatcacggc cgcattcatc ggcttcatcg tcgctgggtt gcccggagca
     1981 ctcgtcggcg ccttcggggt cttcctgccg gcgtacctct tcgtgatcgt gcccggccgc
     2041 tggatcctcc gccacaagga cgcggtcccg gtgcgcgcct tcgtggccgg ggtgagtgcc
     2101 gcggcggcgg gggcgatcct cgctgcatcg gtcattctcg gtcggggcgc gatccgtgac
     2161 ggcgctaccc tcgcgatcgg gctcttggcg ctcgcggcac tgctcgcgat ccggcgccaa
     2221 caactgcggt acatcgaacg cctcgcggag ccgctcgtcg tcgttgtggc aggaatcgcc
     2281 ggacttctgc ttcgcggtgc ctgagccgac ctgggccggc gaggacggca cgatgacgat
     2341 cggtctcaag ccgtgaacgc acctcgcgtc ggcccggcgc cttgccatgg cgtttggagg
     2401 tctcgcggcg cagatgcccc gttcaacgga aaatgcgggg gtgggccaaa cggtagccaa
     2461 gtcggggacg atgaccgccg gacggctccg actggccgaa ctgatcgcgg cgctgtcgct
     2521 ggccacagac ctcggaatgg gccagccgat ggagctggcg ctccgcatcg cggcactcgg
     2581 tgttgcgctc ggccgccggc ttggccttgg gccagccgat ctttcggata tctactatct
     2641 ggcgctggtc gagcacatcg gctgcacctc ggactccctc gaattcgcag ccttctctgg
     2701 cggcgatgac atcggctacc gccggcgggc gatggcgtgg ccggcctcgc cacctggcga
     2761 gatagtcgaa gcgatcgtcc gccatacggg cgagggccgg ccgcccttcg aacgcgcgcg
     2821 tctggttgtg ggcatgctga tccatagcga gcagcggccg cgccaggtcg ttgccgccca
     2881 ctgtgaggcg ggcggccggt tggccgaacg tcttggcctc agccaggggg tccggagggg
     2941 tctgctccaa gaacaggaac ggtgggacgg gaggggcagg cccgagggcc tcgccggcga
     3001 tgctctctgc atggcgcagc gcgtcgtgtc cgttgctcat gacgcgattg tgctcatgga
     3061 agccggccgt cccgtcctgc aaaccctgcg ttccaggcgg ggccacgcgt acgacccggc
     3121 cgtcgttgac gccatggtgg aggcaggccc gcctgcgggt gcaggtgatg ccgacgcctg
     3181 ggtagggggg ttggaagctg agcccgcgcc ggccttgacc gtccccgggg ccgccatcga
     3241 tcgggtcgca cttgcccttg ccgacttcac cgacctcaag tcgccttacc tgctcgggca
     3301 ctccacacgg gtggccgaag tcgcgagcgg ggccgcggac gttctcgcgt gcgcaccggc
     3361 ggaggtcgat gaggttcgtc gcgccgcact cctccacgac ctcggccggg tgggcgtcgc
     3421 gaatgggatt tgggataaga gggggccgct caccgagagc gagagggagc gcgtacgcct
     3481 ccacccctac tacaccgagc ggatcctcgc ccgctcgccg gcactggcgt ccctcgcggt
     3541 catcgcgggc agccaccacg agaacctcga tggctccggt taccaccgcg gggtcggagc
     3601 cacgcagctc ggccggccgg cccgcctcgt tcgggctgcc gactgcctcg acgcgatggc
     3661 ctctgaccgc ccgcaccgag tggcgctgcc agacgacggg cgcatccgcg agctgcgggc
     3721 tgaggtcgac gcgggaagac tcgacgctga ggcggtggcc gcggtcattg aggcgtcggg
     3781 tggcggccgc gtgcacctgc ggccgccgcg accggccgga ctcagcgagc gtgagatcga
     3841 ggtgctccgg ctgctggtgc acggtatcag caacgccgag atcggcactc gcctgcggct
     3901 gtcgcccaag acggtgggtc accacgtcga gcacatttac aacaaggccg gcgtgacatc
     3961 tcgcgctgcc gccgccctct tcgcgatgga aaccgggctc atctgaacaa atggggcgat
     4021 cgcccgatgc gggcaggggg ccgcgcctcg tagggtcacc tccggtgcgg caggtgcgcc
     4081 gcggtatgaa cgggaggcga ccaccatggc gaccacggcg acaacgacag gcctctacca
     4141 tgaagtccgc ggcaccggac ccgcagtgct gctgatccca ggtgccacag gcgacgcggg
     4201 gactttcacg accgccgcca cgcggctggc ggacgagttt acggtcatca cgtacgatcg
     4261 gcgcggcaac tcacgcagcc gcgccacggg cgatccaatg gccgcggcga ccatgtccgc
     4321 gcaggccgat gacgcggccg acctgattgt cgcctgcgga tttaacaagg cggtcgttta
     4381 cgggaacagc ggcggggcga tcattgccct cgagctgctc gctcggcggc cgaccatggt
     4441 aaagggcgca gtcgtgcacg agccaccgct gatcgccctg ctcccgcaag atggcccgaa
     4501 cctgctcgag ccgatcttcc agctggcgca gacggatcca cgggcggcgc ttgaggcctt
     4561 cgtccgagtg aacacgagtg acgccgcgtg ggaggcgatc gattccggca ctcgcgagcg
     4621 catcctcggc aacggcgaga ccctattcgc acgggagatg ggccagttca tctcgtatca
     4681 gcccgacgag gcggtgctgc gggcgcagcg cgttccggtc gtgctgctcc ggagccgcga
     4741 tggcctcgaa ttcgcccccc acgttctggc ctggcttcag gaccgcctcg gagtggaggt
     4801 cggcaccctt tcgggccaac attcaccttt cctggatata cccgaagtgt tcgccgagga
     4861 gctacggccc attctgcgtc ggctctggaa ctgaggatca ccagccccat cacgatcgcg
     4921 ggttcgtgct gacgcttacc tgcccaggtc tgagtccgca attcgctgaa cggcgctctt
     4981 cgagtcgctg gcatggtacg tcctcagcgg accaacgtag acgtccaggc ccacgggttc
     5041 ttctccagga cagatttagc ctgccgggct ggacaaggtt cgctcgatcg cgtcagcgag
     5101 ccggaagtcg cgctcggtga ttccgcccga cccgtgcgtc cagagcgtca gcatcaccac
     5161 gttccagtgg atcgcgacgt caggatggtg gttctgctcg tcggccgggt cggcgatgcg
     5221 cgtaactagc tcgagggctg atcggaagtc gcgcgttttg accgtccgca tcagtcgacc
     5281 atcggcgagc cgccagaggg gcag
//
LOCUS       DATCQR010000052         8330 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_2764, whole genome shotgun sequence.
ACCESSION   DATCQR010000052 DATCQR010000000
VERSION     DATCQR010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 8330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8330
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2764"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..581)
                     /locus_tag="VIN32_01745"
     CDS             complement(<1..581)
                     /locus_tag="VIN32_01745"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HEY8921328.1"
                     /translation="MDLEIAVNAREVYGKQNKRLRTTGVVPGVLFGKKIGSIPVQVNA
                     KALEVLYHQAGRTGVVKVSVDGGQPTSAIIKSVQRNPLTRRPLHVDLFAVDLTQEMLA
                     DIPLTFTGTSPAVELEGAILFTSLDHLKVRGLPGDLPHEIEVDLSPLVDLESTIHVRD
                     LAVDGKVTVLNDPDDLVAKVTPPREEEVIEPVVV"
     gene            724..1419
                     /locus_tag="VIN32_01750"
     CDS             724..1419
                     /locus_tag="VIN32_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010189192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY8921329.1"
                     /translation="MAGTIMIVEDEPAVARGVQVALEQEGYSTAIVGTGEEALKQFAD
                     IAPDLILLDVRLPGIDGFEVCRQLRRETRAPVLFLTARADEVDKVVGLEIGADDYLVK
                     PFSVRELVSRIKALLRRAYGELADTQTGRTLRRGDLVIDLERRRVTRGSERISLTTTE
                     FDLLRHMAAKPGRVYTRTQLLELVRDYEALEADERTINVHISHIRDKIEPDPAQPRFI
                     KTVRGVGYAFGEE"
     gene            1400..2917
                     /locus_tag="VIN32_01755"
     CDS             1400..2917
                     /locus_tag="VIN32_01755"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY8921330.1"
                     /translation="MHSAKSDLPPEPRVGRVGGQLAWFKQLNARLVLALASVALVALL
                     VSGLALNQVLPGYFKEQATSRARTAALATAVYLQEQALSVGADRPELLLVRELRETQF
                     LTAVAERAAKELAQGTVTIYNADANRTTAAQASPDAETAATLRSEGLRPDADVPAQQF
                     STRIQLPDGSRLELLISVTAPYTSRVATLDRVRSTLLFTGLLALIASLLVGIAAARRF
                     TAPLARLRRAASRLAQGQLDERAPRSEIVEIDDLARQFNVMADRLSESLRMLEADRDR
                     LREFVADVSHELRTPIAALRAFTELQRDGVDEATRREFLDRSSEQISRLDWMSTNLLD
                     LSRIDAGIFPLDVRPGDLRDPIRSVVEAHAELAEQRGVSLTSEVPPAPVMLPFDRERI
                     VQLLSNLIGNGLKFTPRGGQVGVALSDHPEGAVLEVRDSGPGILATELPHVFERFFRG
                     TNVGDARASGSGLGLAIARSIVEMHGGQIEVASAIGEGSAFTVRLPRVAPVPQGQ"
     gene            3010..4272
                     /locus_tag="VIN32_01760"
     CDS             3010..4272
                     /locus_tag="VIN32_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEY8921331.1"
                     /translation="MTEHDEPDAQPSEPEVLRYGPPPSQPTRPTWDAPAWSATSAHHT
                     EPRERRTLGVVPVMTIALVTAIISGSLSAAAVANLLRPGSTGVTTEPTGSSVSQVHID
                     ESSAVITAVARVMPSVVVIESRSSGGVLGGANGTGSGFIFDSNGWILTNKHVVQGADQ
                     ISVQLNDSRVFTGRVYGIDTLTDLAIVKIDATGLPAATLGSSEELQLGQLAIAIGNPL
                     GNFENTVTTGVVSGLGRQIQAGDSNGTSSEQLNNLIQTDAAINPGNSGGPLINSAGQV
                     IGINTAVNQDAQGIGFAIPISVARPIVQLALAGKPIARPWIGVYYKPVNAQVAKDLGL
                     SVDHGVLIDKPASGAPAVFAGSPADKAGLQQGDIVVAVDGDVVDENHDLSTRILPHVP
                     GDVLTLSVVRGGKTMEISVTLATLPTTP"
     gene            complement(4269..4634)
                     /gene="folB"
                     /locus_tag="VIN32_01765"
     CDS             complement(4269..4634)
                     /gene="folB"
                     /locus_tag="VIN32_01765"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007166571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEY8921332.1"
                     /translation="MSDRISLRGMQFMGRHGVTAEERAEPQPFEVDLLVRLDLSTPAR
                     TDDLADTVDYAALFTLAREVVEERSFHLLEGLAGAIADGVLAAHPVHDVEVRVRKPRA
                     PLPRALDAAEVRIRRRRGG"
     gene            complement(4631..5485)
                     /gene="folP"
                     /locus_tag="VIN32_01770"
     CDS             complement(4631..5485)
                     /gene="folP"
                     /locus_tag="VIN32_01770"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016483394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEY8921333.1"
                     /translation="MIELRWGERTYVMGIINVTADSFSGDGIATPGRSEAEIVAAAVE
                     QARGFVDAGAQLLDVGGESSRPAQFYGEHLPVSAEEEAALAVPVVAALAAEFGDRALV
                     SIDTSKGPVAREALAAGATIVNDVWAGQRDPETLTAAARSGAYLVLMHNKELAEYPGG
                     VFEEVVSSLRTAIEDALTAGVPRGRLIVDPGIGFGKTPAHSIEILHRLAELKAALGGL
                     PLLVGTSRKRVIGELLGGAPPDQRVEGTAASVALAIAAGADIVRVHDVAAIMKTVRVS
                     DGIVRWQP"
     gene            complement(5482..6792)
                     /locus_tag="VIN32_01775"
     CDS             complement(5482..6792)
                     /locus_tag="VIN32_01775"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HEY8921334.1"
                     /translation="MAALRSRGRFGISLGLERIEALLDELGHPERRLRGALVGGTNGK
                     GSVVALARSALQEAGLRIGTMPKPHLVSYRERIAVDGRPINRERFAAVLGAVTPAVER
                     VTARLGPPTEFEALTAAAFRELAGAGIDLALVEVGLGGRLDATNVLDGGVAVITNVQH
                     DHERLLGSTLAAIGAEKAAIIKRGNLAVTGAQGRGLRPILARCKEVEASLRRAGPGQP
                     YDAALREAGWDGIVVDATRPGGPLPALRIGLLGSHQAANAAVALGLLDALVEDAERDG
                     VPLRLDDGAIRRGFAAARWPGRLELLRETSVGPVLLDGAHNPAGARALARSLEELHLR
                     HFPMVFGAMRGKRVAAVLRALAPLDPVPIFTRVEDRGAIPAEELLGRWRRIGGRGQTT
                     ASPGDALRLAADLRRSAEQPVVVAGSLYLVGAVRGMLRGEEADA"
     gene            complement(6825..7382)
                     /locus_tag="VIN32_01780"
     CDS             complement(6825..7382)
                     /locus_tag="VIN32_01780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921335.1"
                     /translation="MIIKTERRTPPDRRQAADPFAPERPTGDRRTMCWEVENCTLTIR
                     YRCPAFILRRPCWELWAVDGVGPARACCHNEIDCEPGRCAIAEKRFAGTAEPLTVHVG
                     RRGVPLGYGKPRRHSCPHFYLTHGSKGRVGTDSPGVLRMLMKQEGVVNRCELRGGVHL
                     DASYVSDLCMTGQYHDCVFYEDEGA"
     gene            complement(7426..7752)
                     /gene="trxA"
                     /locus_tag="VIN32_01785"
     CDS             complement(7426..7752)
                     /gene="trxA"
                     /locus_tag="VIN32_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019174225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEY8921336.1"
                     /translation="MTAPVTVDDATFEAEVLNAQKPTLVDFWAPWCGPCRLVSPVVEA
                     VGEAHADKIATAKVNTDENQQLAMRYSIFSIPTLIVFRDGREAARLVGYMPQAAMEER
                     LREFLA"
     gene            complement(7839..>8330)
                     /locus_tag="VIN32_01790"
     CDS             complement(7839..>8330)
                     /locus_tag="VIN32_01790"
                     /inference="COORDINATES: protein motif:HMM:NF015734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HEY8921337.1"
                     /translation="LTVPNWVGDPIAGVRAEATRLGLVLDEQPPEPSDTVPVDHVIRT
                     DPTNGTPVTRGDTVQVWVSSGTAQVPVPNLIGQTRQEATDTLHSKRLDLGLVSSAPSD
                     RPAGEVINTNPSAGVKVGVGSQVDLVLSLGPTPSPSPSPTPTPQPTPSPTPVPPPPTP
                     APS"
BASE COUNT         1291 a         2924 c         2877 g         1238 t
ORIGIN      
        1 acgacgacgg gctcgatcac ttcctcctcg cgtggcggcg tgaccttggc gaccaggtcg
       61 tcggggtcgt tgaggaccgt caccttgccg tcgactgcca ggtcgcggac gtggatcgtc
      121 gattcgaggt cgaccagcgg agacagatca acctcgatct cgtggggcag gtcgccgggg
      181 agtccgcgga ccttgaggtg gtcgagcgag gtgaacagga tcgccccctc aagctcgacc
      241 gccggcgagg tgccggtgaa ggtgagcggg atgtcagcaa gcatctcctg cgtcaggtcg
      301 accgcgaaca ggtcgacgtg caaggggcgg cgcgtcagtg ggttgcgctg cacggacttg
      361 atgattgcgc tcgtgggctg accgccgtcg accgagacct tgacaacacc ggttcgcccc
      421 gcctggtggt agagcacctc cagcgccttg gcattcacct gcacggggat tgagccgatc
      481 ttcttgccga acaggacgcc gggcaccacg ccggtggtcc gcaggcgttt gttctgcttg
      541 ccgtacacct cgcgggcatt gacggcgatc tccaggtcca tcggtacggg tgatcctcga
      601 atagctcggc cgggccacgg caggaggaag gtgacgacac tgctgtgcgg ggcggcatcg
      661 taccagcgcg ccgatcgagc ggcaaggagg ccggctgggg atcggtctag aatcccagac
      721 cgaatggcag gcacgatcat gatcgtcgag gatgagcccg cggtcgcgcg gggcgtccag
      781 gttgccctcg agcaggaggg ctacagcacc gccatcgtcg ggaccggcga ggaggcgttg
      841 aagcagttcg ctgacattgc gcccgacctg atcctgctcg acgtgcggct gccggggatc
      901 gacggcttcg aggtctgccg ccagctccgt cgcgagactc gcgctcccgt cctcttcctt
      961 accgcccgcg ccgacgaggt cgacaaggtg gtggggctcg agatcggagc cgatgactac
     1021 ctggtcaagc cgttcagcgt tcgggagttg gtgagccgca tcaaggcgct gctgcgtcgc
     1081 gcctacggcg agctggcgga cacgcagacc ggccgcaccc tgcgccgcgg cgacctggtg
     1141 atcgacctcg agcgaaggcg ggtgacccgc ggcagcgagc ggatcagcct gaccaccacc
     1201 gaattcgacc tgctccgcca catggccgcc aagcccgggc gcgtctacac ccgaactcag
     1261 ctgctcgagc tggtgcgcga ctacgaggcc ctggaggccg acgagcggac gatcaacgtc
     1321 cacatcagcc acattcgcga caagatcgag ccggaccccg cccagccccg cttcatcaag
     1381 acggtgaggg gcgtcgggta tgcattcggc gaagagtgat ctgccgcccg aaccgagggt
     1441 cgggcgcgtc ggcggccaac tggcatggtt caagcagctc aacgcgcggc ttgtgctcgc
     1501 gctcgccagc gtggcgctgg tggctctcct cgtctctggt cttgccctca accaggtcct
     1561 tcccggctat ttcaaggaac aggccacaag ccgcgctcgc acggcggcgc tggccaccgc
     1621 ggtgtatctc caggagcagg cactctcagt gggagccgac agacccgagt tgctgctggt
     1681 tcgggagctg cgcgagacgc agttcctgac ggccgtggcc gaaagggcgg cgaaggagct
     1741 ggcccagggg accgtcacga tctacaacgc cgacgccaac cggaccaccg cggcgcaggc
     1801 ctccccggat gcggagacgg cggccacgct tcgctctgaa gggcttcgcc cggatgcgga
     1861 cgttccggcg cagcaattca gcacgaggat ccaactcccc gacgggtcga ggctcgagct
     1921 gctcatctcg gtcaccgcgc catacacctc gcgcgtggcg acactcgatc gggtgcgcag
     1981 caccctgttg ttcaccggcc tcctcgcgct cattgcctcg ctgctggtcg ggattgccgc
     2041 ggcgcgccgc ttcacagcgc ccctcgcgcg acttcgccgg gcggcgagcc gccttgcgca
     2101 gggacaactc gacgaacgag ctccgcgctc ggagatcgtc gagatcgacg acctggcccg
     2161 tcagttcaat gtcatggcgg accgcctctc ggagtcgctc cggatgctgg aggccgaccg
     2221 cgatcggctc cgcgaattcg ttgcagacgt gtcgcatgag ctgcgcacgc cgatcgccgc
     2281 gctgcgggca ttcaccgagc tccagcgaga tggagtggac gaggccacgc gccgcgagtt
     2341 cctcgaccga tcgagcgagc agatcagcag gctcgactgg atgagcacca acctgctcga
     2401 cctgtcgcgc atcgacgcgg ggatctttcc gctcgacgtg cgtccgggcg acctgcgcga
     2461 cccgatccgc tcagtggtcg aggctcacgc cgagctggcg gagcagcgcg gcgtatcgct
     2521 cacgagcgag gtcccgccgg caccggtgat gctgccgttc gaccgtgagc ggatcgtgca
     2581 gctgctgagc aacctcatcg gcaacggcct gaagttcacc ccacgagggg ggcaggtggg
     2641 cgtcgccctc tccgatcacc cggagggtgc cgtgctcgag gtgcgcgact ccgggccggg
     2701 gatcctggcc acggagctgc cccacgtctt cgagcgtttc ttccgcggca cgaacgtggg
     2761 cgatgcgcgc gcatcgggca gcgggctggg gctggccatc gcccggtcga tcgtcgagat
     2821 gcacggcggg cagatcgagg tcgcgagtgc gatcggcgag ggatcggcct tcacggtgcg
     2881 gttgccgcgc gttgcgccgg tgccgcaagg tcaatgaaac ttcacccctc gctgcgcacg
     2941 ctcgcaaccc gtccagcgca cgatcggcgc tgacgccccc gttcagaaag cagtgagcac
     3001 gaaccagcga tgaccgaaca cgacgagccc gacgcccagc cctctgaacc cgaggtcctg
     3061 cgctacgggc ctccgccgag ccagccgaca cgaccaacct gggacgcgcc ggcgtggtcg
     3121 gcgacttcag cgcatcacac cgaaccccgg gagcggcgca ccctcggcgt cgtcccggtc
     3181 atgacgatcg cactggtgac cgcgatcatc tccggatcgc tctcggcggc tgcggtggcg
     3241 aacctgctgc ggcccggctc cactggcgtg acgactgagc cgaccggcag ctcggtgagc
     3301 caggtccaca tcgacgagtc aagcgccgtg atcacggcgg tggcacgcgt catgccctcg
     3361 gtggtcgtga tcgagtcgcg gagcagcggc ggagtcctgg gcggggcgaa cgggactgga
     3421 tccggcttca tcttcgactc gaacggatgg atcctgacca acaagcacgt cgtccagggc
     3481 gccgatcaga tcagcgtcca gctgaatgac agtcgcgtgt tcaccggccg ggtgtacggc
     3541 atcgataccc ttacggacct ggcaatcgtc aagatcgacg cgaccgggct tccggccgcc
     3601 accctcggct cgagcgagga gctccagctg ggccagctgg cgatcgccat cggcaacccg
     3661 ctcggcaact tcgagaacac ggtcaccacc ggggtcgtct ccgggctggg ccggcagatc
     3721 caggcaggtg actccaacgg cacctcgtcg gagcagctga acaacctgat ccagaccgat
     3781 gccgccatca accccggcaa ctcgggcggg ccgctgatca acagcgccgg gcaggtgatc
     3841 ggcatcaaca ccgcggtcaa ccaggacgcc caggggatcg gcttcgccat cccgatcagc
     3901 gtggcgcgac cgatcgtcca gctggcgctc gccggcaagc cgatcgcccg tccgtggatc
     3961 ggcgtctact acaagcccgt gaacgcgcag gtcgccaagg acctggggct gtcggtcgac
     4021 cacggcgtgc tgatcgacaa gccggccagc ggagccccag cggtcttcgc gggaagcccg
     4081 gcggacaagg cgggcctcca gcaaggtgac atcgtggtgg cggtcgacgg tgacgtggtc
     4141 gacgagaacc atgacctgtc aactcgtatc ctgccgcacg ttcccggcga cgtcctcacc
     4201 ctcagcgtgg tccgcggcgg aaagacgatg gagatcagcg tgacgctggc gaccctcccg
     4261 acgacgccct agccgccgcg gcgacggcgg atccgcacct cggccgcgtc aagggccctg
     4321 ggcaacgggg cccttggctt gcggacgcga acctcgacgt catggacagg gtgggcggcc
     4381 agcacgccgt cggcgatcgc gccggccaga ccctcaagca ggtggaacga gcgctcctcg
     4441 accacctcgc gcgccagggt gaagagggcg gcgtagtcga ccgtgtcggc caggtcatcg
     4501 gtccgggccg gcgtggagag gtccaggcga accagcaggt cgacctcgaa cggctggggt
     4561 tcggcgcgct cctcggctgt gaccccgtgt cggcccatga actgcatccc gcgcaggctg
     4621 atccggtcgc tcatggttgc cacctcacga tcccatctga tacgcgcacc gtcttcatga
     4681 tcgctgccac gtcatggacc cgcacgatgt cagcgcccgc ggcgatcgcc agggccacgc
     4741 tggcggccgt cccctcgaca cgctgatccg gcggcgcgcc accgaggagc tcgccgatga
     4801 ctcgcttgcg actcgtgccg acaagcaacg gcagcccacc cagcgccgcc ttgagctccg
     4861 ccagccgatg gaggatctcg atcgagtgcg ccggtgtctt gccgaagccg atccccgggt
     4921 cgacgatcag ccgtcctcgc gggacgccgg cggtcagggc gtcctcgatg gcggtgcgaa
     4981 gcgacgacac cacctcctcg aacacgccac cggggtactc cgccagctcc ttgttgtgca
     5041 tcaggacgag gtaggctccc gacctggcgg cggcggtcag cgtctcgggg tcccgctggc
     5101 cggcccagac gtcgttcacg atcgtggcgc cggctgcgag cgcctcgcgc gccacggggc
     5161 ccttggaggt gtcgatgctc accagcgcgc ggtcaccgaa ctcggcggcc agcgcagcca
     5221 ccaccgggac tgccagcgct gcctcttcct ctgcagagac aggcaggtgc tccccgtaga
     5281 attgcgccgg ccgggacgac tcgcccccga cgtcgaggag ctgagccccg gcgtccacga
     5341 agccgcgcgc ctgctcaaca gccgccgcca cgatctccgc ctcgctccgg cccggcgtgg
     5401 cgatgccgtc gccgctgaac gagtcggccg tgacgttgat gatccccatc acgtaggtcc
     5461 gctcgcccca gcgcagctcg atcacgcgtc cgcctcctcg ccgcgcagca tcccacgcac
     5521 ggcgccgaca aggtagagcg acccggcaac caccaccggc tgctccgcgg agcggcggag
     5581 gtccgctgcc agccggaggg cgtcccctgg gcttgccgtc gtctgtccgc ggccgccaat
     5641 ccgccgccag cgcccgagca gctcctccgc ggggatcgca ccgcggtcct cgacgcgcgt
     5701 gaagatcggc actggatcga gcggcgccag ggcgcgcagc accgccgcca cgcgcttgcc
     5761 gcgcatcgcg ccgaagacca tcgggaagtg gcgcaggtgc agctcctcga gactgcgagc
     5821 cagggctcgc gcgccagccg ggttatgcgc accgtcgagg agaacggggc caaccgatgt
     5881 ctccctcagc agctcgagcc gccccggcca gcgtgccgcc gcgaagccac ggcgtatcgc
     5941 cccgtcgtcc agacggaggg gaacgccatc gcgctccgcg tcctcgacca gggcgtcgag
     6001 cagccccagg gcgacggcgg cattggcggc ctggtggctc cccagcagcc cgatgcgcag
     6061 agccggcagc ggaccgccgg gccgcgtcgc gtcgaccacg atcccgtccc agccagcctc
     6121 cctgagggcg gcgtcatacg gttgtcccgg cccggctcga cgcagcgaag cctcgacctc
     6181 cttgcagcga gcaaggatcg ggcgaaggcc tcgtccctgg gcaccggtga cggccaggtt
     6241 gccccgcttg ataatcgccg ccttctcggc accgatggca gccagggtcg atcccagcag
     6301 gcgctcgtgg tcgtgctgga cgttggtgat caccgccacc ccgccatcca ggacattggt
     6361 cgcgtccagc cggccgccca ggcccacctc caccagcgcg aggtcgatcc ccgcacctgc
     6421 aagctcgcgg aaggcggcgg cggtcagcgc ctcgaattca gtcggcggtc caaggcgcgc
     6481 agtgactcgc tcgacggccg gcgtgactgc gcccaggacc gccgcgaagc gttcccggtt
     6541 gatcggccgt ccgtcgacgg cgatccgctc gcgatagctg accaggtggg gctttggcat
     6601 ggtgccgatg cgcagcccag cctcttgcag cgcggatcgt gccaaggcca cgacgctgcc
     6661 cttgccgttg gtgccgccga cgagggctcc gcgcagccgt cgctcgggat ggccgagctc
     6721 gtcgaggagt gcctcgatcc gctccaggcc caggctgatg ccaaagcggc cccggctgcg
     6781 cagggcggcc accgccgcct cgtagtccgg gtctgtggac gcggtcaggc cccctcgtcc
     6841 tcgtagaaga cgcaatcgtg gtactggccg gtcatgcaca ggtcgctcac gtagctcgcg
     6901 tcgaggtgga cgccgccgcg cagctcgcat cggttcacga ccccctcctg cttcatcagc
     6961 atgcgcagga cgccgggcga atcggtgccg acgcgaccct tcgagccgtg cgtgaggtag
     7021 aagtgtgggc aggagtgacg gcgcggcttg ccgtagccca acgggacgcc acggcgcccg
     7081 acatgcacgg tgagcggctc ggccgtcccg gcgaagcgct tctcggcgat ggcgcagcga
     7141 cccggctcac agtcgatctc gttgtggcag caggcgcggg ccggacccac gccatccacg
     7201 gcccacagct cccagcacgg ccgccgcagg atgaaggcgg ggcagcgata ccgaatggtg
     7261 agggtgcagt tctcgacctc ccagcacatg gtgcggcgat cgccggtggg gcgctccggc
     7321 gcgaagggat ccgcggcctg gcgccggtcc gggggcgtcc ggcgctcggt cttgatgatc
     7381 accggctgag tctagcgagt gggggcgcgc tcccgcgcat tttccctagg cgagaaactc
     7441 gcgcagccgc tcctccatgg ccgcctgcgg catgtagccc accaggcgag cggcctcgcg
     7501 cccgtcacgg aagacgatga gcgtagggat gctgaagatg ctgtagcgca tcgccagctg
     7561 ctggttctcg tcggtgttca ccttggcggt ggcgatcttg tcggcgtgtg cctcaccgac
     7621 cgcctcgacg accggcgaga cgaggcggca ggggccgcac cagggtgccc agaagtcgac
     7681 cagcgtcggc ttctgcgcat tcaggacctc cgcctcgaag gtcgcgtcgt cgaccgtgac
     7741 cggggctgtc atcttcttgt gttgacctcc aggtcgggat ggtttgcggc cgggatggta
     7801 gcaccgccgc cgcgcacgcg gccgccgcgc ctcggggcct acgacggggc tggcgtcgga
     7861 ggcggcggca cgggtgttgg actgggagtg ggctgaggcg tgggcgtcgg cgacgggctg
     7921 ggcgagggtg tcggtccgag cgagaggacc aggtccacct ggctcccgac gccgaccttc
     7981 acgccggcgc tcgggttggt gttgatcact tcgcccgccg ggcgatcgga cggcgcgctg
     8041 ctgaccagtc cgagatcaag gcgcttggag tgcagggtat cggttgcttc ctggcgggtc
     8101 tggccgatca ggttcgggac cggcacctgc gccgtccccg agcttaccca gacctggacc
     8161 gtgtcaccgc gcgttaccgg cgtgccattc gtcggatcgg tgcggatgac gtggtcgacc
     8221 ggcacggtgt cgctcggctc gggaggctgc tcatcaagga cgaggccaag acgcgtcgcc
     8281 tccgcgcgca cgccggcgat cgggtcgccg acccagttgg gcacggtcag
//
LOCUS       DATCQR010000053        10668 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_64742, whole genome shotgun sequence.
ACCESSION   DATCQR010000053 DATCQR010000000
VERSION     DATCQR010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 10668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10668
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64742"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1035
                     /locus_tag="VIN32_01795"
     CDS             <1..1035
                     /locus_tag="VIN32_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006902699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRCF domain-containing protein"
                     /protein_id="HEY8921338.1"
                     /translation="EDDDGLVRDAINRELDRGGQVFYVHNRVETIEAAAERVRRLVPR
                     AKVAIGHGQMAAGMLERVMLDYDAGRFDVLVCTTIIESGLDIPNANTIVIARADTLGL
                     AQLYQLRGRVGRSDRRAYAYLLHRRGQPLSAVARKRLHAIFSASDLGAGYQIALSDLE
                     IRGAGNILGAEQHGFMAAVGFELYTRMLAEAVEAGRGRAIEPEPAGVRLDLPGSAYLP
                     DDFIADAGAKLEAYRRFAAIHSEADVTALRTELRDRFGPAPEPVEGLFRAVAVRMAAE
                     QAGVPEVRAEAGRVTLKWPRYDRAVVVRALTLAGFRPVAASNQVRIPVPLGRDPVDTA
                     MRALAALGGA"
     gene            1147..3984
                     /locus_tag="VIN32_01800"
     CDS             1147..3984
                     /locus_tag="VIN32_01800"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HEY8921339.1"
                     /translation="MSSQSPLIDPAQARSVLRVGDRSYAYYSLHAAGAPDLARLPYTV
                     KVLLENALRHAAGESSLVSAADVRALAAWDPTQPGEAELPFMPARVILQDFTGVPCVV
                     DLAAMRDAVASMGGDPSRVNPLVPADLVIDHSVQVDLFGSELAFAGNVEREYERNGER
                     YALLRWAQQAFDNFRVVPPGTGIVHQVNLEFLADVVTARPDDSGEQVAFPDTLVGTDS
                     HTTMINGLGVVGWGVGGIEAEAALLGQPLYQPMPVVVGFRLDGELRPGATATDLVLYV
                     TEMLRSHGVVGKFVEFHGPGLSRLGLADRATISNMSPEFGATATLFPIDEETLRYLRM
                     TGRQAETVDRVEAYAKAQGLFRTDESIEPWFNESLSLDLASVEPSVAGPRRPQDRVPL
                     TDLQRSFREVFADGLRNHRLVDEASAESFPASDPPSFNASAPVEDRRMARPEAEAAHA
                     PVEYQPVEVEMGAERFTLRSGSVVIAAITSCTNTSNPSVMVAAGLLARNAVARGLSTP
                     PWVKTSLAPGSRAVTDYLTGAGLMAPLEELGFDLVGYGCTTCIGNSGPLADEIAAAIE
                     ANDLVGVAVLSGNRNFEGRIHPLARASYLASPPLVVAFALAGRADIDLTTQPLGTDRD
                     GRPVMLAELWPSAAEIAEVVAAQVRSEIFTRNYASVFDGDDRWRALPVPAGDRYAWDP
                     ASTYVALPPFFAGIGPEPAPLADVVDARVLAVLGDSVTTDHISPAGSIARTSPAGEWL
                     MEHGVEPREFNSYGARRGHHEVMMRGTFANIRLRNLLTPDAEGNVTEHLASGERMSIF
                     DAAQRYAAEGTQLVILAGKEYGSGSSRDWAAKGPFLLGVRAVIAESYERIHRSNLVGM
                     GILPLQYLPGESAITLGLTGRERFSVTGIADGLRPHQELTVIATSDEGREQRFRAIAR
                     LDGDVDVTYYRNGGVLQTVLRRLAQGSTAGR"
     gene            complement(4191..6533)
                     /locus_tag="VIN32_01805"
     CDS             complement(4191..6533)
                     /locus_tag="VIN32_01805"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY8921340.1"
                     /translation="MTAKVGGRTETRRRDRQVVALVNVVLLVGIGVIAALATEFVPSG
                     HEVTSALAIASGIGVIGILAWSGVGPRRGLVRDLKDADARYRAMVEDLPAVLYVADLG
                     ADATWHYVSPRIQELLGYSAEEWQADTSLWMGHIHPDDRQRVLEDEEFEGRLDIGARS
                     VSEYRMRTRDGREVWIRDEGVVIGNERNEPALYRGFMIDISQQKEAELALRESEEQTR
                     LIIDSASQAYVAIDTQGRVIDWNAQAEVTFGWSRKEALGEPLEDLIIPITQRAAHRAG
                     MARYLATGEGHLVGKRIELTALHRDGHEFLAELTIWPVGSGDDIHFSALIHDITLRKE
                     LEKQLQHQAFHDSLTGLANRALFRDRVAHALARQARGRGAISVLFSDLDDFKTVNDSL
                     GHEAGDQLLVAVAERLRAVMRPEDTTARFGGDEFAILLEETDKEGTRRAAERILEALR
                     SPFEFHGRQVVMRASIGAAITSDGSTEPDDLLRQADLAMYTAKTSGKGRFAFYEPHMH
                     EAAVTRMELKGDLEQAIANQDFELHYQPIVDLRSGNVTGVEALVRWRHPERGLVLPTE
                     FIPIAEETGLVVPLGRWVMAEACRQLAAWTAAGRLSGARLQRLSMWVNVSARELQEPE
                     FVQTVADALESSGIPPERLTLEITESGLMADLDQSTATLHRLRALGARLAIDDFGTGY
                     SSLSYLERLPVEVLKIDRSFTAAIGHGRDVPVLVRSIVKLGQTLHMEVLAEGIETTEQ
                     LTKLRAIDCRLGQGFHFSPALPAVDLIELIAHGWPSAMSA"
     gene            6679..7209
                     /locus_tag="VIN32_01810"
     CDS             6679..7209
                     /locus_tag="VIN32_01810"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEY8921341.1"
                     /translation="MTARSTDDLRAKIREVPDFPKPGILFYDITTLLRDGEAFREVID
                     HMAEAVKGEKIDLVVGMESRGFIFAAPLADRLGAGFVPVRKLGKLPAETIEVEYDLEY
                     GTATLEIHRDAITRGQRVLVVDDLLATGGTVLGTIELVRRLGGEIAGLSFMVELTALG
                     GRAKLGDFAIHTLLAY"
     gene            7220..8080
                     /locus_tag="VIN32_01815"
     CDS             7220..8080
                     /locus_tag="VIN32_01815"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8921342.1"
                     /translation="MAALPVAQLRARSVTDRDEIAAYLRTDRRYAAYALGDLDSASRG
                     RCSWGIAYDADEQPTALAMHHDGLVPQPLFLMGDPEGCRAILASVIKPRDAFFQATEA
                     LDVAASELYELERATILLRMVVDAQTFVPTAGATERLTPDDIEDLNRLYQLGFRAGFA
                     QAILDDAVYYGIRIRGRLVSAAGTHVISRREGIAVVGNVMTHADYRGHGFARMVTGAV
                     TADLLEQVPDVALNVHADNGPAIAAYSRLGYREYRRLTERLGRRRSGGWGLMRPLREA
                     MRITWPRDQR"
     gene            8059..9333
                     /gene="ahcY"
                     /locus_tag="VIN32_01820"
     CDS             8059..9333
                     /gene="ahcY"
                     /locus_tag="VIN32_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEY8921343.1"
                     /translation="MATRPTLKGDVRDIGLADAGALKIEWADRQMPVLAGIRERFAAE
                     RPLAGWKISACLHVTAETANLMRTLAAGGADAVLCSSNPLSTQDDVAASLVEHHGIPT
                     FAIKGEDRDSYYSHISAALDHAPQVTMDDGADLVSELHTSRTELLPDIVGGTEETTTG
                     VIRLRAMAAAGKLRFPVLGVNEALTKHLFDNRYGTGQSAIDGILRATNVLIAGKQVVV
                     GGYGWVGRGIASRAAGMGGIVTVVEVDPIRALEAAMDGYRVTTASVAAEVGDIFIAAT
                     GNLNVWGAADFPKMRDGVILANAGHFNDEFELSALESQAASSREVRPFTREYLMADGR
                     RLFLLADGRLVNLGAAEGHPALVMDMSFANQALGLEWLRSHAGELKPEVYGIPDEIDR
                     EVARLKLESMGIEIEPMTKAQKAYSESWESGT"
     gene            9707..9886
                     /locus_tag="VIN32_01825"
     CDS             9707..9886
                     /locus_tag="VIN32_01825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921344.1"
                     /translation="MTEPLAPLPPPPLLAAAWLGWLPVLGSPPWLYAPGLVPGGPEQA
                     AMRAAMASSARCGRR"
     gene            10009..>10668
                     /locus_tag="VIN32_01830"
     CDS             10009..>10668
                     /locus_tag="VIN32_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019123241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HEY8921345.1"
                     /translation="MSIPEGHTLFTSESVTEGHPDKLCDQIADAVLDDVLSRDPESRV
                     AVEVAATTGLVFVLGEMSTKTYCDVQSVVRDTVRQVGYDNARLGFDWETCGTITALKE
                     QSPDIALGVDHALEAKQGIDPDELGAGDQGMMFGYASDETPELMPMPIQLAHQVTRRL
                     AEVRRAGDLPFLRPDGKSQVTVEYDADRRPRRVHTVLVSTQHDPEVAPLELEEGVREL
                     VI"
BASE COUNT         1625 a         3693 c         3656 g         1694 t
ORIGIN      
        1 gaggacgacg acggcctggt gcgggacgcc atcaaccgcg agctggaccg tggcgggcaa
       61 gtcttctacg tccacaaccg ggtggagacg atcgaggcgg cagccgagcg ggtccggcgc
      121 ctggtgccgc gggccaaggt cgccatcggc catggacaga tggcggcggg gatgctcgaa
      181 cgggtgatgc tcgactacga cgccggccgc ttcgacgtcc tggtgtgcac caccatcatc
      241 gagtcgggtc tcgacatccc gaacgctaac acgatcgtca tcgcccgggc cgatacgctc
      301 gggctggcgc agctctacca gctccgcggc cgggtcggcc gctcggaccg acgcgcctac
      361 gcctatctgc tgcaccggcg gggacagccg ctctcggccg ttgcgcggaa gcggctgcat
      421 gcgatcttct cggcctccga cctgggggcg ggctaccaga tcgcgctctc ggacctcgag
      481 atccgggggg ccggcaacat cctgggcgcc gagcagcacg gcttcatggc cgccgtcggc
      541 ttcgagctgt acacccgaat gctggcagag gcggtcgagg ccggtcgtgg gcgagcgatt
      601 gaacccgagc ccgcgggtgt ccgcctcgac ctgcctggct ccgcctacct gcccgatgac
      661 ttcatcgccg atgccggtgc caagctggag gcctatcggc gcttcgccgc gatccacagc
      721 gaggccgatg tgactgcgct gcggaccgag ctgcgcgacc gattcggccc cgctcccgag
      781 ccggtggagg ggctcttccg ggcggtcgcg gtgcgcatgg ccgccgaaca ggcgggggtg
      841 cccgaggtgc gcgccgaagc ggggcgagtc acactcaaat ggccgcgcta cgaccgcgcc
      901 gtggtcgtcc gcgccttgac gctggcgggc ttccgaccgg tcgccgcgtc gaaccaggtg
      961 cggatcccgg tgccgcttgg ccgcgacccg gtcgacaccg cgatgcgtgc cctcgccgca
     1021 ctgggcgggg cctagatagg ggcgtatact ccgaattgat cactcgaccc gggacgggcg
     1081 cacccgtccg gcgccgaccg ctctgatacc gcgctccatg cgcggatgac gaaggaggcg
     1141 cgtccggtgt ccagtcaatc tcccctcatc gatcccgccc aggcccgttc cgtgctgcgc
     1201 gtcggcgatc gaagctatgc ctattacagc ctccacgccg caggcgcccc cgacctggca
     1261 cgcctgccgt acacggtcaa ggtcctgctc gagaatgcgc tgcgccatgc cgctggagag
     1321 tcgagcctcg tgtccgcggc cgacgtgcga gccctcgctg cctgggatcc cacgcagccc
     1381 ggagaggcgg agctcccctt catgccggcg cgcgtgatcc tgcaggactt caccggcgtt
     1441 ccttgcgtgg tcgacctggc cgccatgcgc gacgccgtgg cctcgatggg tggcgacccg
     1501 tcgcgcgtca acccgctggt gcctgccgac ctggtcatcg atcacagcgt gcaggtcgac
     1561 ctgttcggct cggagctggc cttcgccggc aacgtcgagc gcgagtacga gcggaatggc
     1621 gagcgctacg ccctgctgcg ctgggcacag caggccttcg acaacttccg ggtcgtccct
     1681 ccggggaccg gcatcgtgca ccaggtcaac ctcgaattcc tggccgatgt cgtgaccgcc
     1741 cgacccgatg acagcggcga gcaggtcgcc ttcccggaca ccctcgtggg caccgactcc
     1801 cacaccacca tgatcaacgg cctgggggtc gtcggctggg gggtcggggg gatcgaggcc
     1861 gaggccgcgc tccttggtca gccgctctac cagccgatgc ccgtggtggt cggcttccga
     1921 ctcgacggcg agctgcgccc cggggcgacc gccaccgacc tggttctgta cgtgaccgag
     1981 atgcttcgtt cccacggcgt ggtcggcaag ttcgtggaat tccacggtcc cgggctctcg
     2041 cgcctcggcc tggccgatcg ggccaccatc agcaacatgt cgcctgagtt cggcgcgacc
     2101 gccaccctct tcccgatcga cgaagagacc ctgcgctacc tgcgcatgac cgggcgccag
     2161 gccgagacgg tggaccgggt cgaggcctac gccaaggcac agggcctctt ccggaccgat
     2221 gaatccatcg agccgtggtt caacgagtcg ctcagcctgg acctcgcgag cgtcgagcca
     2281 tcggtcgcag ggccccgccg cccgcaggac cgcgtgcccc tgaccgatct gcagcgctcc
     2341 ttccgcgagg tattcgccga cgggctcagg aaccatcgcc tcgtggacga ggcgagcgcc
     2401 gaatcgttcc cggccagcga ccctccctcg ttcaacgcca gcgcgcccgt cgaggaccgg
     2461 cggatggcac gccccgaggc ggaggctgct catgcgccgg tggagtacca gccggtcgag
     2521 gtcgagatgg gcgctgagcg attcacccta cgctcggggt cggtggtaat cgccgcgatc
     2581 acgtcgtgta ccaacacgag caacccgagc gtgatggtgg ccgccgggct cctcgcccgc
     2641 aacgccgtgg cgcgcggtct gagcaccccg ccctgggtca agacatcgct cgcacccggt
     2701 tcacgggcgg tcaccgacta cctcactggt gctggcctga tggctcctct cgaagagctc
     2761 ggcttcgacc tggttgggta cggttgcacg acctgcatcg gcaactccgg gccgctggcc
     2821 gacgagattg cggcagcgat cgaggcgaac gacctggtcg gcgtggcggt cctctcgggc
     2881 aaccgcaact tcgagggccg aatccatccg ctggcacggg ccagctacct cgcatcaccg
     2941 ccgctggtgg tggcgtttgc cctcgccggg cgtgccgaca tcgacctcac gacccagccg
     3001 ctggggaccg atcgcgacgg tcggccggtc atgctcgctg agctgtggcc atcggcggcg
     3061 gagatcgcgg aagtggtcgc cgcgcaggtt cggtccgaga tcttcacgcg caactacgca
     3121 tcggtcttcg acggagatga ccgctggcga gcgctgccgg tgccagccgg cgatcgctat
     3181 gcctgggacc cagccagcac ctacgtcgcg ctgccgccct tcttcgccgg gatcgggcca
     3241 gagccggcgc cgctcgcgga cgtcgtcgat gccagggtgc tggcggtgct cggcgacagc
     3301 gtcacgaccg accatatctc accggcaggc agcatcgccc gcacctcgcc ggccggggag
     3361 tggctgatgg agcacggggt cgaaccgcgc gagttcaact cgtacggggc ccgacgcggc
     3421 catcacgagg tgatgatgcg cggcaccttc gccaacatcc ggctccgaaa cctgctgacg
     3481 cccgatgcgg aggggaacgt caccgagcac cttgcctccg gggagcggat gagcatcttc
     3541 gacgctgcgc aacggtatgc cgcggaggga acccagctcg tcatcctggc cggcaaggag
     3601 tacggctccg gctcgtcgcg ggactgggcc gccaaggggc cgtttctgct cggcgtgcgc
     3661 gccgtcatcg cggagtcata cgagcggatc catcggtcga acctggtggg aatgggcatc
     3721 ctgccgctgc aatacctgcc aggggagagc gccatcacgc tcggcctgac cgggcgcgag
     3781 cgcttcagcg tcaccggcat cgccgacggg cttcggccgc accaggagct gaccgtgatc
     3841 gcgacttctg acgagggtcg cgagcagcga tttcgcgcaa ttgcccggct cgacggcgac
     3901 gtcgacgtga cctactaccg caacggggga gtgctccaga cggtgctgcg ccgcctcgcg
     3961 caagggtcca cggccggtcg ctgatctcga gcggcggcta ttcagtagcg gactggaagg
     4021 tcgacggagc aacccgcgac cgggcctccg ccgatcgctg attcacctgg tggttggcgc
     4081 gtcgggaacg cggcggctcg cgcccgctcg gccgacacag cagtgaacgt gtgaatagaa
     4141 gggcataccc ggcgcctcaa tcgcggcccg atgcggtcag cagaggcccg tcaggcgctc
     4201 atcgccgacg gccagccgtg ggcgatgagc tcgatcagat cgaccgcggg cagcgccggg
     4261 gagaagtgga agccctggcc cagcctgcag tcgatcgccc gcagcttggt cagctgctcg
     4321 gtcgtctcga tcccctcggc cagcacctcc atgtgcagcg tctggcccag cttgacgatc
     4381 gagcggacca gaacggggac gtcgcgtccg tgaccgatcg cggccgtgaa ggagcggtcg
     4441 atcttcagca cctcgaccgg cagccgctcc aggtagctga gtgacgaata gccggtgccg
     4501 aagtcatcga tcgccagtcg cgcaccgagc gcgcgcagcc ggtgcagggt ggcggtgctc
     4561 tggtccaggt ccgccatcag gccgctctcg gtgatctcca gtgtcagacg ctccggcggg
     4621 atcccactcg attcgagcgc atccgccacg gtctgcacga actccggctc ctggagctcg
     4681 cgcgccgaca cgttgaccca catcgacagg cgctgcagcc gagctccgga caggcgtccg
     4741 gcggcggtcc acgcagcgag ctgtcggcag gcctcggcca tcacccagcg gccgagcggc
     4801 acgaccagtc cggtctcctc ggcgatgggg atgaactcgg tcggaaggac gagcccgcgc
     4861 tcggggtggc gccaccggac gagtgcctcg accccggtca cgttgccgct ccgcaggtca
     4921 acgatcggct ggtaatgcag ctcgaagtcc tggttcgcga tagcctgctc caggtcgccc
     4981 ttgagctcca ttcgggtcac ggcagcctcg tgcatgtgcg gctcgtagaa cgcgaagcgg
     5041 ccctttccgg aagtcttggc ggtgtacatc gccaggtccg cctggcgcag gaggtcatcg
     5101 ggctccgtgc tgccgtccga cgtgatcgcg gccccgatgc tggcccgcat gacgacctgg
     5161 cggccgtgga actcgaatgg cgagcggagc gcctccagga ttcgctcggc ggcgcgccgc
     5221 gtgccctcct tgtcggtctc ctccagcagg atcgcgaatt cgtcgccgcc gaatcgggct
     5281 gtggtgtcct cgggtcgcat cacggctcgc aggcgctcgg cgaccgccac cagcaactgg
     5341 tcgccggctt cgtggccaag cgagtcgttg acggtcttga agtcgtcgag gtcggagaag
     5401 aggaccgata tggcgcctcg accgcgagcc tgccgcgcga gggcgtgggc gacgcggtct
     5461 cggaacaggg cgcggttggc gaggccggtc agcgagtcgt ggaaggcctg gtgctggagc
     5521 tgcttctcga gctctttgcg gagcgtgatg tcgtggatca gcgcgctgaa gtggatgtca
     5581 tccccgctcc cgaccggcca gatggtcagc tccgccagga actcgtggcc gtcccgatgc
     5641 agcgccgtga gctcgatccg cttcccgacc aggtggccct cgccggtggc caggtagcgt
     5701 gccatcccgg cgcggtgagc cgcgcgctgc gtgatcggga tgatcaggtc ctcgagcggc
     5761 tcacccagcg cctccttgcg ggaccagccg aaggtcacct cggcctgcgc gttccagtcg
     5821 atgacgcggc cctgggtgtc gatggcgaca tacgcctggc tggcgctgtc gatgatcagg
     5881 cgcgtctgct cctcgctctc ccgaagggcc agctcggcct ccttctgctg cgagatatcg
     5941 atcatgaagc cgcggtaaag ggccggctcg ttgcgctcgt tgccgatcac cactccctcg
     6001 tcgcggatcc agacctcgcg accgtctcgg gtccgcatgc ggtactcgct caccgacctg
     6061 gcgccgatgt cgagtcgacc ttcaaattcc tcgtcctcga ggacccgctg ccgatcgtcg
     6121 gggtggatgt ggcccatcca caggctcgtg tctgcctgcc attcctcggc gctgtagccg
     6181 agcagctcct ggatgcgggg gctgacgtag tgccaggtcg catcggcacc cagatcggca
     6241 acgtagagga ccgctggcag gtcttcgacc atggcccggt agcgcgcatc ggcatccttg
     6301 agatcgcgga ccaggccacg gcgcggcccg acgcccgacc aggcgaggat gccgatgacg
     6361 ccgatcccgc tggctattgc cagggctgat gtgacctcgt gcccactggg gacgaactcg
     6421 gtggcgagcg cggcgatgac tccgattcca acgagcagca cgacgttcac gagcgcgacg
     6481 acctgtcgat cgcggcgcct cgtctcagtg cgcccgccga ccttcgctgt cacgggtgga
     6541 acgcgctcca aatcaccccc ggacggtacc cttgcgccct cacgacccga atagcccgtt
     6601 ggtcccgccc aacgtccccg cgggaggacg catcgggcta gactcaggct cgcccgtttg
     6661 ccccacctgg agtgccccgt gaccgctcgc tccaccgacg acctgcgtgc caagatccgc
     6721 gaagtgcccg acttcccgaa gccggggatc ctcttctacg acatcaccac gctcctgcgt
     6781 gacggggaag cgtttcgaga ggtgatcgac cacatggcgg aggcggtcaa gggggagaag
     6841 atcgacctgg tggtcgggat ggaatcccgc gggttcatct tcgcggcgcc gctggcggac
     6901 cgcctgggag ctgggttcgt cccggtgcgc aagctcggaa agctgccggc cgagacgatt
     6961 gaggtcgagt acgacctcga gtacgggacc gcgaccctgg agatccatcg cgacgccatc
     7021 acccgcgggc agcgggtcct ggtggtcgac gacctgctgg ccaccggtgg aacggtgctc
     7081 ggcacgatcg agctggtgcg ccggctcggc ggcgagatcg cggggctctc gttcatggtc
     7141 gagctcacag cgctcggtgg gcgtgccaag ctcggcgact tcgcgatcca cacgctgctc
     7201 gcctactagt cgcccactga tggccgccct cccggtcgcc cagctccgag ctcgctccgt
     7261 caccgatcgg gacgagatcg cggcctacct gcgcactgac cgccgctatg ccgcctacgc
     7321 cctgggtgac ctcgactcgg cgtcacgcgg ccgctgctct tgggggatcg cctacgacgc
     7381 cgacgagcag cccaccgcgc tcgcgatgca ccacgacggc ctggtgccgc agccgctctt
     7441 cctgatgggc gatcccgaag gctgccgggc catcctggcc agcgtcatca agccgcgcga
     7501 cgcgttcttc caggcgacgg aagccctgga cgtggctgcc tcggagctct acgagctcga
     7561 gcgggccacg atcctgctgc ggatggtggt cgacgcgcag accttcgtcc cgacggccgg
     7621 ggccaccgag cgcctgacgc ccgacgacat cgaggatctg aatcggctgt atcagctggg
     7681 cttccgggcg ggatttgctc aggcgatcct tgacgacgcg gtctactacg ggattcgcat
     7741 ccgcggccgg ctggtgagcg ctgccggcac ccacgtcatc agtcgtcgcg aggggatcgc
     7801 cgtggtcggc aacgtgatga cgcacgccga ctaccgcggc cacggctttg cgaggatggt
     7861 gaccggcgcg gtgaccgcgg acctgctgga gcaggttccc gacgtggcgc tcaacgtcca
     7921 cgccgacaac gggccggcca tcgcggccta ctccagactc ggctaccgtg agtaccgccg
     7981 gctgaccgag aggctggggc gccgccgatc cggcggctgg gggttgatgc gaccgttacg
     8041 agaggcaatg cgaatcacgt ggccacgaga ccaacgctga agggcgacgt tcgcgacatc
     8101 ggcctggccg atgcgggggc gctgaagatc gaatgggcgg accgccagat gccggtcctc
     8161 gccgggatcc gcgagcgctt tgcggcggag cgaccgctgg ccggctggaa gatcagcgcc
     8221 tgcctgcatg tcaccgccga gactgcaaac ctgatgcgca cattggcggc gggcggcgcc
     8281 gatgccgtcc tctgctcgtc gaacccactc tccacccagg atgacgttgc ggcatcgctg
     8341 gtggagcacc acggcatccc caccttcgcc atcaagggag aggatcggga ttcgtactac
     8401 agccacatct cggcagccct ggaccacgcg ccccaggtga cgatggacga tggggcggac
     8461 ctcgtgtctg agctgcatac cagtcgcacg gagctactcc ctgacatcgt tggcgggacc
     8521 gaggagacga ccaccggcgt gatccggttg cgggccatgg cggcggccgg caagctgcga
     8581 ttcccggtgc tgggcgtcaa cgaggcgctc acgaagcacc tcttcgacaa ccgctacggg
     8641 acaggccaga gcgcgatcga cgggatcctg cgcgccacga acgtgctgat cgccggaaag
     8701 caggtggtgg tcggtggcta cggctgggtc ggtcgtggga tcgcctcccg cgcggcgggc
     8761 atgggcggca tcgtgaccgt ggtcgaggtt gacccgatcc gggcgctgga ggcggcgatg
     8821 gacggctatc gggtcaccac cgcatcggtc gccgcggagg ttggcgacat cttcatcgcg
     8881 gccaccggca acctgaacgt ctggggcgcg gccgacttcc cgaagatgcg cgatggcgtg
     8941 atcctggcca acgccggcca cttcaacgac gagttcgaac tgtccgcgct ggagtcgcag
     9001 gcggcctcct cacgggaagt gcggcccttc acgcgcgagt acctgatggc cgatgggcgc
     9061 cgcctcttcc ttctggccga tgggcgcctc gtcaacctcg gcgctgccga ggggcacccc
     9121 gcgctggtca tggacatgag cttcgccaac caggctctcg gcctcgagtg gctgcgctcg
     9181 cacgccgggg aactcaagcc ggaggtctac ggcatccccg atgagatcga ccgcgaggtt
     9241 gcgcgcctca agcttgagtc gatggggatc gagatcgaac ccatgacgaa ggcacagaag
     9301 gcatacagcg agtcttggga atccggcacc tgaccgcgat cgggccttcg tctacgggtg
     9361 tgcgtcccaa tcggccgtaa ggtgagcctt gccggtcccg ctcggttgta ccactccggc
     9421 gctcgtgcac tcgacatgac cggagagacc gctggcatcg ttcttggtca gcgtgaaggt
     9481 acagccggag cccgcactgg actcgccgac gccgatgacc acggcggtcc catcgccgaa
     9541 gctgaagatc tggccctgag agaccgtagt cagcgagacg acggttgtgg cgccggttgt
     9601 ttccgcgaac gaggccgacc atcccttggt gttcgagtcg aatacgctgg ccagtggttg
     9661 gaacggcagc tcacccgcgg ctgtgtagtc ccctgttatc tgataggtga ccgagccgtt
     9721 ggcgccgctt cccccgcccc cgctgctggc agccgcctgg ctgggctggc tccccgtgct
     9781 cggctcgccg ccctggctct acgccccggg actcgttcct ggcgggcctg agcaggcggc
     9841 gatgagggcc gccatggcca gcagcgcgag atgtgggcgc aggtgacgca tcggtccggc
     9901 tccctcggcg agatgggctg cgaactgggc tgccggcatg ctacccgcaa cgcagtcgga
     9961 ggggaaggtc ggcgcactgc gcccgccgac ggtacactcg gccccgcaat gtccatcccc
    10021 gagggccata cgctcttcac ctccgagtcg gtgacggagg gtcatcccga caagctctgt
    10081 gaccagatcg cggatgcggt cctggacgac gtcctctcgc gggaccccga gtcacgcgtg
    10141 gccgtcgagg ttgccgccac gaccggcctc gtcttcgtgc tcggcgagat gtcgaccaag
    10201 acctactgcg acgtgcagtc ggtggtgcgc gacaccgtac gccaggttgg ctacgacaat
    10261 gcgcgtctgg gcttcgactg ggagacgtgc gggaccatca ccgcgctcaa ggagcagtcg
    10321 cccgacatcg cgctcggcgt cgaccacgcc ctcgaggcga agcaggggat cgacccggac
    10381 gagcttggcg ccggggatca ggggatgatg ttcggttacg cctcggacga gacgccggag
    10441 ctgatgccga tgccgatcca gctcgctcac caggtgaccc gcaggctggc cgaggtccgt
    10501 cgcgccggcg accttccgtt cctgcggccc gacggcaaga gccaggtgac ggtcgagtac
    10561 gacgccgatc gacgcccgcg ccgggtccat accgtcctgg tcagcacgca gcacgatccc
    10621 gaggttgccc cgctcgagct cgaggagggt gtccgcgagc tggtgatc
//
LOCUS       DATCQR010000054        12459 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_188512, whole genome shotgun sequence.
ACCESSION   DATCQR010000054 DATCQR010000000
VERSION     DATCQR010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 12459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12459
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188512"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..671
                     /locus_tag="VIN32_01835"
     CDS             <1..671
                     /locus_tag="VIN32_01835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921346.1"
                     /translation="TALTASGEAPAPELTAAAEVVAGASDEADAARRELLALAGVAAA
                     VEPAAIVPTLSHGGAELELIAAQLRSSADAATLFVERRHATQAVVDALAAALAALERD
                     DPSAALASLAKADAPMALLGAWERPPPLLRYWMTISTGLLEAAGDIARATLAEDPVAQ
                     KAGAERYARAAEAARGADNALALALSEEGAAVSATPLRRLAAVADEVDEARAALRLAR
                     LRAS"
     gene            739..2220
                     /locus_tag="VIN32_01840"
     CDS             739..2220
                     /locus_tag="VIN32_01840"
                     /inference="COORDINATES: protein motif:HMM:NF013079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M17 family peptidase N-terminal
                     domain-containing protein"
                     /protein_id="HEY8921347.1"
                     /translation="MRVRADSSKPASVQADVLAVPIYLEDREPPPDLTELDEATGGAI
                     TRTLTWGEFNPIEDETALVEVDGIKAARILFVASGRRGRGAWRARRNASKATRRLQGR
                     GATSLAFWLRDGEDADGYAAAAIGASQGTFRPYAFYGRVRDTPAMLRSVEELILVGQE
                     APDDGVLSEAMAIADGVEWARELSNRSANDLYPEKMAELARGLVDDGCTVEVLGPDEM
                     QRLGMGALLGVGMSSSHEPRLIAVKLPGWEAGGDRRLAIVGKGVCFDSGGISLKGPER
                     MEEMKHDKAGAAAVLAAVRTVARLAPQTPLMAVAPMVENMPSGTAQRPGDVVKAMNGK
                     TIEVINTDAEGRLILADALHWAEKQGATHMVDVATLTGAAAVAFGDMISTYFARPREW
                     GSEVAAAAHATGEWFWEMPLATEYRDTLDSAHADIVNSASREGSLIKSAVFLSEFVTV
                     PWVHIDIGGSAYLIRDKAHNPKGSLGTTVSTLVRLARDFAAEA"
     gene            2226..2885
                     /locus_tag="VIN32_01845"
     CDS             2226..2885
                     /locus_tag="VIN32_01845"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HEY8921348.1"
                     /translation="MLAALVAGIAFAAVGAGAERLASVWPADEASHRGPGLRTVLLAI
                     GAGMAGGAVTWRSTLPAWALAVHLLILAVLVVLTATDLEQRRLPHLLLDSLIVVAALF
                     VPFNPTVAWSDALIGGVAAVAFMGVLGLMVRGGVAFGDLYLVGPIGLVLGWPAIFVAV
                     FVAGLLSAVVSALLLVTRRAGLKTYIPFGPFLVAGMVVTLLRDPALLGGVAAALSGAP
                     S"
     gene            3042..4340
                     /locus_tag="VIN32_01850"
     CDS             3042..4340
                     /locus_tag="VIN32_01850"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEY8921349.1"
                     /translation="MSIASRPAAPWVAAFLLLTFAAGPLMSQAMVRATDPTVDGAISE
                     QLQMQAELQRQRTQLADLQREQTNLTASLMSLSDDLTRVGLEIEQAARDIEQAARRLE
                     EARAELKKYEDEIATLEQTLVELAGGIKVSKVELAAREALLQEHMRTAYEQSQTSVLE
                     ILLSTESFTKASNELSSMLTLSDEDRRLADEIRNTRTRLEVRSETLSVGRGTLAKLRD
                     TAAARADALAEQQRQLDAARAALEKKRTQLEGMRGAQEARFDTVTQSADTQRQLIAAQ
                     EQQLLAQQSLVDKLKAVADRLDIAYRGRFDWPERGAFIVTQEFGPTNFNTFHTGLDMA
                     YLNRCGGAIYAAGDGIVIADGRPNSKYGDFAIGVIIGHSQRLATLYWHLSREIVTVGQ
                     EVHVGDVIGYEGATGFATGCHLHFEVDFDGTAVNPRKYLP"
     gene            4404..7223
                     /locus_tag="VIN32_01855"
     CDS             4404..7223
                     /locus_tag="VIN32_01855"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEY8921350.1"
                     /translation="MHPAMQRRRHRLMIRRTTQRRSPRRGLATFVMVVVGVFVLFIGG
                     SIAGTGAGLLAAYGYFSSGLPDPHIIDGIAMPASTYVYDRTGKQLLARFECENREEVK
                     FAQLPDSIVNATVAAEDRTFWTNDGIDYTAVARAALANLQAGSVVQGASTITQQVIKY
                     AGSIKQAQATTEPLPSAAPSVELDPGSQPAAADPCQQPDLTFLGGRGFGDKIREQILA
                     RQVTEAYPGRAGKERILETYLNLIYYGNRSYGIKAAAANFFGQPDLSKLTLSQGALLA
                     GLPQLPSYYDPYQPVAAPRGPARVIARRNEVLDAMLHEAYITKAQHDEAVAVTWEQMN
                     PSKVTSVLREPHFAFRVQHEVERILEAMGVPNPEQAVRTGGYRITTTLDYPLQQVAKE
                     QVVKWVAGVADKNVHNGALVAIDSATGEIVAYVGSVDYYNRKDPRVQGQFDVAGLGRR
                     QPGSAFKPITYSSAFRAREATPATFFLDAVTQYGSDLKTAYVPTNANIKEQGPVLAMD
                     ALRYSLNVPSVMMQYLVGVDVTAKFAQSMGIASSDYIMSQDPGLTLTLGSVPVNLTNM
                     TQAYSVFAAEGTLHPATAVLRILDRHGKVIYDITKNGPGKTQPMTPAEAYLTHWILEG
                     NTNPRTNQLWGPPSQLFDPSGQRRHAALKTGTTNDFRDVSAFGYIPGSLVTGVWMGNN
                     NQEPLSNKLGQGLYSADGPLYLWHDFMKLAINQAWDWNGKKPAPNNDFVQPPGVVMAS
                     VCRFSGMSPTGACGATIEVPFLDGTVPPRDNVHVNKSSRLGGSCFDVVAEVAQDGRRP
                     PEMVAAAIRWSDRFVNGQLGPRGDPSQIGVLGPDKVWLLISRLRGNSGFGAPICGTIR
                     ATPKPSPTPVPSGGGGGGGGGCAPGKPGKCTPAPTPAQSAALSGNAPVQPAGLVAIFA
                     VPAILGTVPMAARIGRWGRRRLRPR"
     gene            7239..8498
                     /locus_tag="VIN32_01860"
     CDS             7239..8498
                     /locus_tag="VIN32_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEY8921351.1"
                     /translation="MAQSILTLVLAGGEGERLSILSQVRAKPGVPFGGKYRIIDFALS
                     NAVNSGLSDVAVLTQYAPRSLIDHIGVGRPWDLDRSRGGVTLLQPYLGRGRSRDWYRG
                     TADAVLQNVEFIADRNPELVVILAGDHVYKMDYRPFIERHRRSGADITCAVRTVPIED
                     AHRFGILEADEDGWITAFVEKPARPTSNLVSMGVYVFGWPQLRAVLTGDRVDFGRDVL
                     PAMVAAGKRVSAYHYGGYWQDVGTVESYWQTSLDLLSDNPGIDLYDRGWLIYTKSEER
                     APALIGPEANVTRSMVSHGCVIHGTVDHSILSPGVRVEAGAVVRDSIVMFDTVIGPGA
                     TVDRAILDKDCVIGPGSRVGDGDDLRPNRQEPERLFAGITLVGKRARIPGGIVIGRNC
                     RIDPSVEAADFGRRRSIRSGTTVTHAD"
     gene            8545..9066
                     /locus_tag="VIN32_01865"
     CDS             8545..9066
                     /locus_tag="VIN32_01865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921352.1"
                     /translation="MIGGGGTSGDAAFGRDILLDGARRFDLRATVAWVEGVGLSAWAY
                     YGDEEMEVPKRVYARMLRANYDYPFVKFALTDDDRPMLMTELPPDGLDRDALARSLVR
                     LTVVADRLLDETAVAIADHGKLPDWSGRTPRNAALLAAHRVEVEASMPAWEPTPPRPP
                     RRSLLDRLLGSGR"
     gene            9063..11000
                     /locus_tag="VIN32_01870"
     CDS             9063..11000
                     /locus_tag="VIN32_01870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921353.1"
                     /translation="MSRALATAVSFAVLVLPLVPPSPANAAEYELATVADYAVDPAAG
                     AIAVTVSVKFTNTLPDPPGQISAFTHVDLAIQDGASGVAASDDAGRLSVAIQPRDGGQ
                     VASVATRSRVRYNASVNLTLTYRLADGAARDLHVGPAVVKFPAWGFGTSSQVTVRLPA
                     GFEAVAEGGPLRTAEDAAGVVLTSGPIPDPRDWLAVVTAVPPPDYVTQAASVPLASGT
                     VDLQVRAWRSDASWGERTLALLTEALPLLEEAIGLPYPRVGPLVVSEAAGGEPGTGAL
                     PSANAEIQVAFDGTAFTLLHQAAHVWIADQLASDRWVREGLASHYAAAAAARLSVEPP
                     YHPATRARELATDAMPLLDWVGDAGASADAYGYAASWELVDRIASAVGEARLSMALRR
                     IAAGLSAYDPTEPNGDGTDGRPVPAVDTRRFLDQLAAVSSVDVAGLFRETVFGLDAAA
                     ELSQRDAARAAYRRLLTAAGDWGASDPVRAAMSEWRFDDALAGIAQASAWLTERDALI
                     AKADAAGLVAPDRLRDRYLLSGGGPEALAELEAEGALVDAYAAVQKRTLAQRAPLEAV
                     GLLLADDPGRLLAEAADDFGQGDLRAAAGALDRLELQLDRASSDGAVRLAAALVLLAL
                     VGLALAVALRRRSGSHYTAAG"
     gene            10997..12058
                     /locus_tag="VIN32_01875"
     CDS             10997..12058
                     /locus_tag="VIN32_01875"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEY8921354.1"
                     /translation="MTTEEGTAKRVVVPPRFLPAADVLAGETADVYFERARRILAAEG
                     MDPVVTMEIFAREEAVLCGAEEAHAYLRAILGGNGHPTGDPAPLVESLHDGDRIAPKE
                     VVMRITARYSAFGLYETAILGILSQSTGWATAARRVVEAAAPIPVIGFGARHVHPSVA
                     DQMDYASVVGGCVGASTPAGARLAGLAPTGTMPHALIIIFGDTVRAAEAFDRHIDPDV
                     PRIVLVDTFKDEAEESLRVADALGDRLWGVRLDTPSERGRVTADLVKEVRARLDLGGH
                     QRVKIVVSGGLDLDRIAYFKASEAPIDSFAVGSAISDASPIDFTGDLKEIDGKPVAKR
                     GRIPGRTENPRLQRVDLES"
     gene            complement(12055..>12459)
                     /locus_tag="VIN32_01880"
     CDS             complement(12055..>12459)
                     /locus_tag="VIN32_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018045565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IB"
                     /protein_id="HEY8921355.1"
                     /translation="RVVGRCQDLPGQELFAYVDEAGEVRSVGSSDVNDYIREISGHDF
                     TAKDFRTWSATVLAARALARGTSPDTAALARRDVVGAIEQVAEWLGNTSAVSRKSYVH
                     PSVIDAYLEGDVIRKGSEAAVLALLRRRAKGR"
BASE COUNT         1875 a         4340 c         4377 g         1867 t
ORIGIN      
        1 gcacggcgct gaccgcctcc ggtgaggcgc cagctccgga gctgaccgcc gcggccgagg
       61 tcgtcgctgg cgccagcgat gaggcagacg ctgcccgtcg ggaactgctg gcgctggccg
      121 gagtcgccgc cgcggtcgag ccggcggcga tcgtccccac cctctcccat ggtggggccg
      181 agctggagct gatcgccgcc cagctgcgat ccagcgccga cgcggcgacc ctgttcgtgg
      241 aacgacgcca cgcgacgcag gccgtcgtcg acgccctggc cgcggcgctg gcggcgctcg
      301 agcgcgacga tccctcggcg gcgctcgcca gcctcgcgaa agccgatgcg cccatggcgc
      361 tcctgggggc ctgggagcgg ccgccgccgc ttctgcgcta ctggatgaca atcagcaccg
      421 gtctcctgga agccgccggg gatatcgcga gggcaacgct ggccgaggat cccgtggcgc
      481 aaaaggcagg ggccgagcgc tatgcccggg cggcggaggc ggcgcgcggt gccgacaacg
      541 cgctggcgct ggcactctcc gaggagggcg ccgccgtcag cgcaacgccg ctgcggcgtc
      601 tcgcggcggt cgcggacgag gtggacgagg cgcgcgccgc cctcagattg gcgcggctgc
      661 gggcctcctg agcccccgcc aggccgccct atactggcgc gaccgacccc cctggccaag
      721 cgatctcgag gagcatcgat gcgcgttcgt gcggatagca gcaagccggc atcggtccag
      781 gccgacgtgc tggcggtgcc gatctacctc gaggaccgcg agccgccgcc cgaccttacc
      841 gagctcgacg aggccaccgg cggcgccatc acccggacgc tgacatgggg cgagttcaac
      901 ccgatcgaag atgagacggc cctcgtcgag gtcgatggga tcaaggcagc gcggatcctg
      961 ttcgtggcct cgggacgacg tgggcgtggg gcgtggcgcg ccaggcgaaa tgcatcgaag
     1021 gccacccgca ggctgcaggg ccgcggcgcc acgagcctcg ccttctggct gcgtgacggc
     1081 gaggacgccg atggatacgc ggctgccgcc atcggcgcca gccagggcac ctttcgaccg
     1141 tacgccttct acggcagggt gcgcgacact ccggccatgc tgcgcagcgt cgaggagctg
     1201 atcctggtcg gccaggaagc gccggatgac ggcgtgctga gcgaggcgat ggccatcgcc
     1261 gacggggtcg agtgggcgcg tgagctgtcg aaccggtcag ccaacgacct ctacccggag
     1321 aagatggccg agctcgcccg gggcctggtc gacgatgggt gcaccgtcga ggtgctgggt
     1381 ccggacgaga tgcagaggct cggcatggga gccctgctcg gggttgggat gagctcgtct
     1441 cacgagccgc gcctcatcgc ggtgaagcta cccggctggg aggcgggggg tgatcgccgg
     1501 ctggcgatcg tcggcaaggg agtctgcttc gactcgggcg ggatcagcct gaaggggccg
     1561 gagcggatgg aggagatgaa gcacgacaag gccggcgcgg cggcagtgct ggcggctgtg
     1621 cggaccgtgg cgcggctggc gccccagact ccactcatgg cagtcgctcc gatggtcgag
     1681 aacatgccca gcggcacggc ccagcgcccg ggagacgtgg tcaaggcgat gaacggcaag
     1741 acgatcgagg tgatcaatac cgatgccgag ggccggctga tcctggccga tgccctccac
     1801 tgggcggaga agcagggcgc aacgcacatg gtcgatgtcg cgaccctgac cggcgcggcc
     1861 gccgtcgcgt ttggcgacat gatcagcact tacttcgcca ggccgcggga atgggggagc
     1921 gaggtggcgg cagccgccca tgcgacgggc gagtggttct gggagatgcc gctcgccacg
     1981 gagtaccgcg atacgctcga ctcggcacac gccgacatcg tgaactccgc ttctcgagag
     2041 ggatcgctga tcaagagcgc cgtcttcctt tcggagttcg tcactgtgcc ctgggtccac
     2101 atcgatatcg gcggatccgc ctacctgatc cgcgacaagg cgcacaaccc caagggttcg
     2161 ctgggaacca ccgtctcgac gctggtccgc ctcgcacgcg acttcgccgc cgaggcgtag
     2221 cgttgatgct cgccgcgctg gtcgctggca tcgccttcgc cgccgtcggc gctggtgcgg
     2281 aacggctcgc aagtgtgtgg ccggcggacg aagcctcgca tcgaggaccg gggctgcgga
     2341 ctgtcctcct ggccatcggc gcgggcatgg cggggggagc cgtgacctgg cgctcgacgc
     2401 ttcccgcctg ggcgttggcg gtccacctcc tgatcctggc cgtgctcgtc gtgctgaccg
     2461 caacggatct ggagcagcgc cggctgccgc acctgctgct cgactcgctc atcgtggtcg
     2521 ccgcactctt cgtcccgttc aatccgacag tcgcgtggtc ggacgcgctc atcggcggcg
     2581 tcgcggcggt tgccttcatg ggcgtgctgg gcctgatggt gcgcggcggg gtggcgttcg
     2641 gcgacctcta cctggtcggc cccatcggcc tcgtgctcgg atggcccgcc atcttcgttg
     2701 ccgtcttcgt ggccggcctt ctttcggcgg tcgtcagcgc attgctgctc gtgactcggc
     2761 gtgcgggact caagacctac atcccgttcg ggccattcct cgtcgccggc atggtggtca
     2821 ctctcctgcg cgatccggct ctgctgggtg gggtcgccgc cgccctctcc ggcgcacctt
     2881 catgaggtag cgcacgccgc cggatctgca ttgacagccc gctggacagg tcgggtattc
     2941 ttggcctccc cggccgagcg cctcggggtc gctccgagtt ccgcgcgcag gcccctggcg
     3001 tcgcggcccg aagcaggtac cgccagcgac accagggagt aatgtcgatc gccagtcgcc
     3061 cagccgcgcc atgggtcgcc gccttcctgc tgctgacgtt cgcggctggt ccgctgatgt
     3121 cgcaggcgat ggtgcgtgcc accgatccga cggtcgacgg cgccatctcc gagcagctcc
     3181 agatgcaggc cgagctccag cgccagcgca cccagctggc cgacctgcag cgcgagcaga
     3241 ccaacctgac cgcgagcctg atgagcctga gcgatgacct gacccgggtc gggctcgaga
     3301 tcgagcaggc ggcgcgcgac atcgagcagg cggcgcgacg gcttgaggag gcgcgggccg
     3361 agcttaagaa gtacgaggat gagatcgcga ccctcgagca gaccctcgtg gagctggcgg
     3421 gcgggatcaa ggtctcgaag gtcgagctgg ccgcgcgcga ggcgctcctc caggagcaca
     3481 tgcgcaccgc ctacgagcag agccagacat cggtcctcga gatcctcctc tcgaccgagt
     3541 cgttcaccaa ggcctcgaac gagctctcgt cgatgctcac cctctccgac gaggaccgca
     3601 ggctcgccga tgagatccgc aacacgcgca ctcggctcga ggtccgcagc gagaccctga
     3661 gcgtcgggcg cggcaccctg gcgaagctgc gcgacaccgc tgccgccagg gccgatgcgc
     3721 tggccgagca gcagaggcag ctggacgccg cgcgtgcggc actcgagaag aagcgcacgc
     3781 agctggaggg gatgcgcggc gcccaggaag cacgcttcga caccgtcacg cagagcgccg
     3841 acacgcagcg acagctgatc gcggcccagg agcagcagct cctcgcacag cagtcgctgg
     3901 tcgacaaact caaggcagtt gctgaccgcc tggatatcgc ctaccggggg cgcttcgact
     3961 ggcccgaacg aggtgcgttc atcgtcaccc aggaattcgg cccgaccaac ttcaacacct
     4021 tccataccgg gctcgacatg gcctacctga accgatgcgg cggagcgatc tacgccgctg
     4081 gggatggcat cgtgatcgcc gatggtcgcc cgaactcgaa gtacggcgac tttgcgatcg
     4141 gcgtgatcat cgggcattcg cagcgcctgg ccaccctgta ctggcacctg tcgcgtgaga
     4201 tcgtgacggt cggccaggag gtgcacgtcg gggacgtcat cgggtacgag ggcgccaccg
     4261 gcttcgcgac cggttgccac ctgcacttcg aggtcgattt cgacgggact gcggttaacc
     4321 cgaggaagta cctgccctga gccgaggccc gtcgccaacg gcctgattcg accgattccc
     4381 gcgcatgggg tagggttcgc ccgatgcacc cggccatgca gcggcgccgt catcggctga
     4441 tgatccgccg caccacccag cgtcgcagcc cacgacgcgg gctcgcaacg ttcgtcatgg
     4501 ttgtggtcgg cgtgttcgtc ctgttcatcg gcggctcgat cgccgggacc ggcgccggcc
     4561 tgctcgccgc gtacggctac ttctccagtg gcctgcccga tccgcacatc atcgacggca
     4621 tcgcgatgcc ggccagcacc tacgtctacg accggaccgg gaagcagctg ctggcccgtt
     4681 tcgaatgcga gaaccgcgaa gaggtgaagt tcgcgcagct gcccgactcc atcgtgaacg
     4741 cgaccgtcgc cgccgaggac cgcaccttct ggacgaacga cgggatcgac tacaccgccg
     4801 tcgcccgcgc ggcgctggcg aacctccagg ccggctctgt cgtccagggc gccagcacca
     4861 tcacccagca ggtgatcaag tacgcgggct cgatcaagca ggcgcaggcg acgaccgagc
     4921 cgctgccctc ggcggcgccg tcggtcgagc tcgacccggg cagccagcct gccgcggccg
     4981 atccgtgtca gcagccggac ctcaccttcc tcgggggccg cggcttcggt gacaagattc
     5041 gcgagcagat ccttgcacgc caggtgaccg aggcctatcc ggggagggcc ggcaaggagc
     5101 ggatcctcga gacctatctc aacctgatct actacgggaa ccgctcctac ggcatcaagg
     5161 ccgctgcggc caacttcttc gggcagccgg acctgagcaa gctgaccctt tcgcagggcg
     5221 ccctgttggc gggcctgccg cagctcccca gctactacga tccctaccag ccggtcgcgg
     5281 ctccacgggg tccggcccgg gtcatcgccc gccgcaacga agtgctcgac gccatgctgc
     5341 acgaggccta catcaccaag gcacagcacg acgaggcggt ggcggtcacc tgggagcaga
     5401 tgaacccgag caaggtcaca tcggtcctgc gtgagcctca cttcgcgttc cgcgtccagc
     5461 acgaagtcga gcgcatcctg gaggcgatgg gcgtgcccaa ccccgagcag gcggtccgaa
     5521 ccggcggcta ccggatcacc accacgctcg actatccgct gcagcaggtt gcgaaggagc
     5581 aggtcgtcaa atgggttgcc ggcgtggccg acaagaacgt gcacaacggc gccctggtgg
     5641 ccatcgactc cgcgaccggc gagatcgtgg cctacgtggg cagcgtcgac tactacaacc
     5701 gcaaggaccc gcgtgtccag gggcagttcg acgtggccgg cctgggacgc cgccagccgg
     5761 gatcggcctt caagccgatt acctacagtt cggccttccg cgcccgggag gcgaccccgg
     5821 cgaccttctt cctggacgcc gtgacgcagt acggctcgga cctcaagacc gcatatgtcc
     5881 cgaccaacgc caacatcaag gagcagggcc cggtgctcgc gatggatgcg ctgcgctact
     5941 cgctgaacgt cccctcggtg atgatgcagt acctggtcgg ggtggacgtg acggccaagt
     6001 tcgcacagtc catgggcatc gccagctcgg attacatcat gagccaggat ccgggcctga
     6061 ccctcaccct cggctcggtg ccggtcaacc tgaccaacat gacccaggcc tacagcgtct
     6121 tcgccgccga gggcacgctc cacccggcca ccgccgtgct gcggatcctc gaccgccacg
     6181 gcaaggtgat ctacgacatc accaagaacg gccccggaaa gacgcagccg atgacccccg
     6241 ccgaggcgta cctgacgcac tggatcctgg agggcaacac caaccctcgg accaaccagc
     6301 tgtggggccc gccctcccag ctgttcgacc ccagcgggca gcggcgtcac gcggcgctga
     6361 agacgggcac caccaacgac ttccgcgatg tctcggcgtt cgggtacatc cccggcagcc
     6421 tggtcaccgg cgtctggatg gggaacaaca accaggagcc actttcaaac aagctcgggc
     6481 aggggctgta cagcgccgac gggccgctct acctgtggca cgacttcatg aagcttgcca
     6541 tcaaccaggc gtgggactgg aacggaaaga agccggctcc gaacaacgac ttcgtgcagc
     6601 cgccgggggt cgtcatggca tcggtctgtc gcttcagcgg catgtcgccg accggtgcat
     6661 gcggcgcgac catcgaagtt ccgttccttg acgggaccgt cccgccccgt gacaacgtgc
     6721 acgtcaacaa gtcgtcgcga ctgggcggct cgtgcttcga cgtggtggcc gaggtcgccc
     6781 aggacggccg ccggccgcct gagatggtcg cggcagccat acgctggtcc gatcggttcg
     6841 tcaacggcca gctgggcccg aggggggatc ccagccagat cggggtcctg ggacccgaca
     6901 aggtgtggct cctgatctcg cgactgcgag gcaacagcgg ctttggcgcg ccgatctgcg
     6961 gcacgatccg cgccacgccg aagccctctc ccactcccgt gccgagcggc ggtggcggtg
     7021 gcggtggcgg cggatgtgcc cccggcaagc cggggaagtg cacgcccgcg cccacgcccg
     7081 cgcagtcagc ggcgctgtcc ggcaacgcac cggtgcagcc cgcggggctc gtggcgatct
     7141 tcgccgtgcc cgcaattctg gggacggtgc cgatggccgc ccgcatcggt cgttgggggc
     7201 gccgccgact tcgccctcgc tagtgcgaga atcacggcgt ggcacagtcg atcctgaccc
     7261 tggtcctggc aggcggcgag ggtgagcggc tctcgatcct gtcgcaggtc cgtgccaagc
     7321 ccggcgttcc gttcggcggc aagtaccgga tcatcgactt cgcgctgtcg aacgcggtca
     7381 actcggggct gagcgacgtc gccgtcctga cgcagtacgc gccgcgctcc ctgatcgacc
     7441 acatcggcgt cgggcggccc tgggacctcg accgcagccg gggcggggtc accctgctgc
     7501 agccgtacct cggacgcggc cgttcgcgcg actggtaccg cgggacggcg gacgcggtcc
     7561 tgcagaacgt ggagttcatc gccgatcgca acccggagct ggtggtgatc ctcgccggcg
     7621 accacgtcta caagatggac tatcggccgt tcatcgagcg gcatcggcgc agcggggcgg
     7681 acatcacctg cgccgtgcgc acggtgccga tcgaggatgc gcatcggttc ggcatcctcg
     7741 aggccgacga ggacggttgg atcacggcct tcgtcgagaa gccggccagg ccgaccagca
     7801 acctggtcag catgggcgtc tacgtcttcg gctggccgca gctccgcgcg gtcctgaccg
     7861 gggaccgggt cgacttcggc cgggacgtgc ttcccgccat ggtcgctgcc ggcaagcgcg
     7921 tttccgcgta ccactacggg gggtactggc aggatgtcgg aacggtcgag agctactggc
     7981 agaccagcct cgacctgctc tccgacaacc ccgggatcga cctctacgac cgcggctggc
     8041 tgatctacac caagagcgag gagcgcgctc cggcgctgat cggcccggaa gcgaacgtga
     8101 cgcgcagcat ggtgagccat ggctgcgtga tccacggaac ggtcgaccac agcatcctgt
     8161 cgccgggcgt gcgtgtcgag gcgggcgcgg tggtgcggga ttcgatcgtg atgttcgaca
     8221 cggtcatcgg cccgggcgca accgtcgacc gcgccatcct cgacaaggac tgtgtcatcg
     8281 gtcccggcag cagggtgggg gatggagatg acctgcggcc gaaccgacag gagcccgagc
     8341 gtctgtttgc cgggatcaca ctcgtcggca agcgcgcccg catcccgggc gggatcgtca
     8401 tcgggcgcaa ctgccggatc gacccgagcg ttgaggcggc cgacttcggg cggcgccgca
     8461 gcatccgatc gggcacgacg gtgacccacg ctgactgacg aagctccgcc gctcgacgac
     8521 tggctggcgg agctgggcgt agaggtgatc ggtggcgggg gcacgtccgg cgacgccgcg
     8581 ttcggccgcg acatcctgct ggacggcgcc cggcgcttcg acttgcgggc caccgtggcc
     8641 tgggtcgagg gcgtgggtct ctccgcctgg gcgtactacg gcgacgagga gatggaggtg
     8701 ccgaagcggg tctatgcccg catgctcagg gccaattacg actacccgtt cgtgaagttc
     8761 gccctgaccg atgacgaccg tccgatgctg atgaccgagc ttccgcccga tggactcgat
     8821 cgtgacgcgc tggcccgcag cctggtgcgc ctgacggtcg tcgccgaccg gctcctggat
     8881 gagaccgccg tggcgatcgc cgaccacggc aagctgcccg attggagcgg tcgaaccccg
     8941 cgcaatgccg ccctgctggc agcgcatcgg gtcgaggtgg aagccagcat gccggcctgg
     9001 gagccgacgc cgccacggcc tccgcggcgc agcctgcttg accgattgct gggcagcggc
     9061 cgttgagtcg cgccctggcg accgccgtct ccttcgccgt gctggtcctt ccgctcgtcc
     9121 ctccgtcccc cgcgaacgcc gccgagtacg agctggcaac ggtcgccgac tacgccgtcg
     9181 accccgctgc cggcgcgatc gcggtgaccg tgtccgtcaa gttcaccaac acactcccgg
     9241 atccgcccgg ccagatcagc gccttcacgc atgtggatct cgccatccag gacggggcgt
     9301 ccggggtggc ggccagcgac gacgcggggc ggctctcggt cgccatccag ccgcgcgatg
     9361 gggggcaggt ggcgtcggtc gcgaccaggt cgcgcgtgcg ctacaacgcc tcggtcaatc
     9421 tcaccctcac ctatcggctg gcagacggcg ccgcccggga tctgcacgtg gggccggcgg
     9481 tcgtgaagtt ccccgcctgg gggttcggca ccagcagcca ggtcacggtc aggctgccgg
     9541 ccggcttcga ggcggtcgct gaaggcggcc cacttcgcac cgcggaggat gcagccgggg
     9601 tcgtgctgac cagcggcccg atcccggatc cgcgtgattg gctggcagtc gtcaccgccg
     9661 ttcctccgcc cgactacgtg acgcaggctg ccagtgttcc cctcgccagc gggacggtgg
     9721 acctgcaggt ccgcgcctgg aggagcgatg cttcgtgggg ggagcggacg ctggcgctcc
     9781 tcactgaggc attgccgctg ctggaggagg cgatcgggct gccctatcca cgtgtcgggc
     9841 cgctggtggt cagcgaggcg gccggcggcg agcctggcac gggcgcactg ccgtcggcga
     9901 atgccgagat ccaggtcgcg tttgacggga cggcctttac gctgctgcac caggccgcgc
     9961 atgtctggat cgctgaccag ctggcctctg atcgctgggt gcgtgaaggg ctcgcctcgc
    10021 actacgcggc cgctgccgcc gcgcggctca gcgtcgagcc tccgtaccat ccggccacgc
    10081 gtgccaggga gctggcgacg gacgccatgc cactgctgga ctgggttggc gatgccggcg
    10141 cgtcggccga tgcctacgga tacgcggcgt cctgggagct cgtcgaccgg atcgcgagcg
    10201 cagtggggga ggcccgcctg agcatggcgc tcagacggat cgcagcgggg ttatcggcct
    10261 acgacccgac cgagccgaat ggcgatggca ccgatgggcg cccggtcccg gccgttgaca
    10321 cgcggcgatt cctcgatcag ctggccgcgg tgagcagcgt ggacgtcgcg ggcctcttcc
    10381 gcgagacggt gttcgggctc gatgcagccg ccgagctctc gcagcgggac gcggctcggg
    10441 ccgcctaccg tcgcctgctg accgccgccg gcgactgggg cgcctccgac ccggtgcgcg
    10501 ccgccatgag cgagtggcga ttcgacgatg ccctggcggg gatcgcgcag gcatccgcct
    10561 ggctgacgga acgcgatgcg ctgatcgcca aggctgatgc agccggcctc gtcgcgcctg
    10621 accggctgcg ggatcggtat ctcctgtcgg ggggcggtcc cgaggccctt gccgagctcg
    10681 aggcggaagg ggcgctggtg gacgcctacg ccgccgtcca gaagcgcacg ctggctcagc
    10741 gagccccact ggaagcggtc ggcctcctcc ttgccgacga tccggggcgg ctgctggccg
    10801 aggcggcgga tgacttcggg cagggggacc tgcgcgcggc ggcgggcgcg ctcgatcggc
    10861 tggagctgca gctggaccgt gcgtcctcgg acggggccgt gcggctcgcg gcggcgctgg
    10921 tgctgctggc gctggtgggc ctggcccttg ccgtggcgct tcggcgccgc tccggcagcc
    10981 actacactgc tgccggatga cgaccgagga gggcacggcg aagcgggtgg tcgtgccgcc
    11041 gcgcttcctg ccggccgccg acgtcctggc cggcgagacg gccgacgtct acttcgagcg
    11101 ggctcgccgc atcctggccg ccgagggcat ggatccggtc gtcaccatgg agatcttcgc
    11161 ccgcgaggag gcggtcctgt gtggcgcaga ggaggcgcac gcctacctgc gcgcgatcct
    11221 cggcggcaac gggcacccca ccggagaccc cgctccgctg gtcgagtccc tgcacgacgg
    11281 cgacaggatc gcgccgaagg aggtggtgat gcggatcacg gctcggtact cggcattcgg
    11341 cctgtacgag accgcgatcc tgggaatcct ttcgcagagc acgggctggg cgaccgccgc
    11401 ccgccgagtg gtggaggcag cggcgccgat cccggtcatc gggttcgggg cacggcacgt
    11461 gcatccctcc gtcgccgacc agatggacta cgcctcggtg gtgggtggct gcgtcggagc
    11521 ctcgacgccc gccggcgcac gccttgccgg cctcgctccc accgggacca tgccgcacgc
    11581 cctgatcatc atcttcggcg acacggtccg cgccgccgag gcgttcgacc gtcacatcga
    11641 tcccgacgtg ccgcgaattg tgctggtaga taccttcaag gacgaggcgg aggagtcgct
    11701 gcgcgtggcc gatgccctcg gcgaccggct gtggggggtt cggctcgaca ccccctcgga
    11761 gcgcggtcgg gtcaccgccg acctcgtcaa ggaggttcgc gcccggctcg acctgggcgg
    11821 ccaccagcgc gtgaagatcg tggtctcggg tggtctcgac ctggaccgga tcgcctactt
    11881 caaggcgtcg gaggcgccga tcgactcgtt cgcggtcgga tcggccatct cggatgcctc
    11941 gccgatcgac ttcactggcg acctgaagga gatcgacggc aagccggtcg ccaagcgcgg
    12001 ccgcatccct ggtcgaaccg aaaacccgcg gctgcagcga gtggacctgg agtcctagcg
    12061 gcccttcgcg cgcctgcgca gcaaggccag caccgccgcc tccgagccct tgcgaatcac
    12121 gtcgccctcc agatacgcgt cgatcacgct cgggtgcacg taggacttgc gcgacaccgc
    12181 ggacgtgttg ccaagccatt ccgccacctg ctcgatcgcc cccaccacgt cgcgcctggc
    12241 cagcgccgcg gtgtcgggtg acgtccccct cgccagcgcc cgcgcggcga gcacggtggc
    12301 ggaccaggtg cggaagtcct tggccgtgaa atcgtggccc gagatctcgc gaatgtagtc
    12361 gttgacgtcg ctggacccga ctgaccggac ctcgccggcc tcgtccacat acgcgaacag
    12421 ctcctggccc ggcaggtcct ggcagcggcc gacgacgcg
//
LOCUS       DATCQR010000055         2345 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_157603, whole genome shotgun sequence.
ACCESSION   DATCQR010000055 DATCQR010000000
VERSION     DATCQR010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157603"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..821)
                     /locus_tag="VIN32_01885"
     CDS             complement(<1..821)
                     /locus_tag="VIN32_01885"
                     /inference="COORDINATES: protein motif:HMM:NF013673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HEY8921356.1"
                     /translation="MVEALGWDSAALESLLSRALSLATDAGAEAEALFTERSAALTRF
                     ANSRIHQNVAERDATLRIRLVRDARTGVASTNRLDDEGLREAVGRASATLALATPNPD
                     GAPLAAPSPSSADPRLGFVAATARADPELRAVGARAVIAAGDAAHLESSGAFSTETAT
                     LAVANSRGMRNTHSVTQAKLLTVMMDGKGGSGYAQATGPDIRAIDPTAVGEEAAGKAS
                     RSHDAVDLEPGDYPVILEEYAVATVLEYLSWMGFSALAVEEGRSFMDLGQQIMGA"
     gene            complement(815..2263)
                     /locus_tag="VIN32_01890"
     CDS             complement(815..2263)
                     /locus_tag="VIN32_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEY8921357.1"
                     /translation="MQELTDLALDVARSAGAGYADMRIVERATESLTVKNGNLAEASS
                     NRSAGFGVRVLLDGAWGFAGSSRMERAEVERTAREAVAIARASGLATREPIVLDDSPA
                     AVGVYHTPYREDPFEVTLDEKLGLLFEADAVMGRVAGVTLRTSSMEFGRERKTFVSTE
                     GARIEQEIVDSGAGIEAMAFNEHEVQRRSYPQSAGGQHVAGGFEAIRDLGLADNAQRI
                     AEEAVALLDAPQCPTGQMTLIIDSPQLALQVHESCGHPIELDRVLGMEASFAGTSFLT
                     LDKLDQLQYGSEIVNMEADATAPGGLGTFGFDDEGVAAQKVPIVTAGRLVGYLTSRET
                     APIIGRRSMGSSRASGWNVIPLIRMTNVNLRPGDAGTLDDLIADTSDGLFVSTNHSWS
                     IDDRRLNFQFGTEVGWLIKNGRLTQMVKNPTYTGITPRFWGSCDAICGPSEWTLWGVP
                     NCGKGEPMQTHGVGHGAAPARFRGVDVGVGGW"
BASE COUNT          332 a          850 c          778 g          385 t
ORIGIN      
        1 ttcgcgccca tgatctgctg gccgaggtcc atgaacgacc gtccctcctc caccgccagt
       61 gccgagaagc ccatccacga caggtattcg aggacggtcg ccacggcgta ctcctcgagg
      121 atgaccgggt agtcgccggg ctcgaggtcg accgcgtcgt ggctgcgcga ggccttgccc
      181 gccgcctcct caccgacggc cgtcgggtcg atggcgcgaa tgtcggggcc ggtcgcctgc
      241 gcgtagcctg accccccttt gccgtccatc atcaccgtca gcagcttggc ctgggtgacg
      301 ctgtgcgtgt tacgcatgcc acgtgagttg gccaccgcca gggtggcggt ctcggtcgag
      361 aaggctccgc tggactcgag gtgcgcggca tcgccggcgg caatcacggc cctggcgccc
      421 acggcgcgca gctccggatc ggcccgggcg gtggcggcca cgaagccgag ccgggggtcg
      481 gcgctggacg ggctcggcgc cgccagtggt gcgccgtccg ggttgggggt tgcgagcgcc
      541 aaggtcgccg aagcgcgccc aaccgcctcg cgcaacccct catcgtcgag ccggttggtg
      601 ctggccacgc cggttcgtgc atcacgcacc agccggatcc gcaatgtggc gtcgcgctca
      661 gcgacgttct ggtggattcg cgagttggca aagcgcgtga gtgccgcgct tcgctcggtg
      721 aagagcgcct ccgcctcggc accggcatcg gttgccagcg aaagcgcgcg gctgagcagc
      781 gattcgagcg ccgccgaatc ccagccgagc gcctctacca tccacccacc ccgacgtcga
      841 caccgcggaa gcgggccggg gcggcgccgt ggccgacgcc atgcgtctgc atcggctcac
      901 ccttgccgca gttgggcacg ccccatagcg tccattccga cgggccgcag atcgcgtcgc
      961 agctccccca gaagcgcggc gtgatgccgg tgtaggtcgg gttcttgacc atctgcgtca
     1021 ggcgtccgtt cttgatcagc cagccgacct cggtcccgaa ctggaagttg aggcgtcggt
     1081 cgtcgatgct ccaggaatga ttggtactga caaagaggcc gtccgaggtg tccgcgatga
     1141 ggtcgtcgag cgtaccggcg tcgcccggcc gcaggttgac attcgtcatg cggatgagcg
     1201 gaatcacgtt ccagcccgat gcccggctgc tgcccatgct gcgacggccg atgatcggcg
     1261 cggtctcccg gctggtgagg tagccgacca ggcgccctgc cgtgacgatc ggcacctttt
     1321 gcgctgccac cccctcgtcg tcgaagccga aggtgccgag cccccccggc gcggtcgcat
     1381 cggcctccat gttcacgatc tcgctcccgt actggagctg gtcgagcttg tcgagggtca
     1441 ggaagctggt tcccgcgaag ctcgcctcca tgccgagcac gcggtcgagc tcgatcgggt
     1501 ggccgcagga ctcatgcacc tggagcgcca gctgcgggga gtcgatgatc agcgtcatct
     1561 ggcccgtggg gcattgcggc gcatcgagca gcgctaccgc ctcctcggcg atgcgctgcg
     1621 cgttgtcagc cagaccgaga tcgcggatcg cctcgaagcc gccggccacg tgctgaccac
     1681 ccgccgactg cgggtagctg cgccgctgca cctcatgctc gttgaaggcc atcgcctcga
     1741 tccccgcccc ggaatcgacg atctcctgct cgatgcgggc gccctcggtc gagacgaagg
     1801 tcttgcgctc ccgaccgaac tccatgctgc tggttcgcag cgtcacgccc gcgaccctgc
     1861 ccatgaccgc atcggcctcg aacagcaggc ccagcttctc gtcgagagtc acctcgaagg
     1921 gatcctcgcg atacggggtg tggtagaccc ccaccgccgc cggcgagtca tcgaggacga
     1981 tcggctcgcg cgtcgccagg ccactggccc gcgcgatggc gaccgcctcc cgcgccgtcc
     2041 gctccacctc ggcgcgttcc atgcgtgagc tgccggcgaa tccccacgct ccgtcaagca
     2101 acacccgaac gccgaacccg gcagaccggt tggagctcgc ctccgccagg tttccgttct
     2161 tgacggtcag cgactcggtg gcgcgttcga cgatgcgcat gtccgcatag cccgcgccgg
     2221 cgctgcgggc tacgtcgagg gccaggtcgg tcagctcctg catcgcttcc tcattccacc
     2281 ggcttgccga tcaccggctg cagcgagacc gcgctcgccg gcagctgcac cgatgttgcc
     2341 tgccg
//
LOCUS       DATCQR010000056         2453 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_64963, whole genome shotgun sequence.
ACCESSION   DATCQR010000056 DATCQR010000000
VERSION     DATCQR010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2453
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64963"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            87..773
                     /locus_tag="VIN32_01895"
     CDS             87..773
                     /locus_tag="VIN32_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014141154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEY8921358.1"
                     /translation="MVEVAPGTIVVFSDIGCPWAHVSVWRLHDARRRLGLEGRVAFDH
                     RVFPLELFNSQPTPRDELAVEWEACASLAPRAGWQAWSAPDWAWPATMLPPMEAVQAA
                     KEQGLGASEELDRGLRRAFWGESRCVSLRHVILEVAAECDGLDLEALAEAFDSGRART
                     ALFDQYEVARSDAVDGSPHLFLADGTNVENPGIKATWEEGSDGFWRPTVVEDDPSAYD
                     RLVTAAAVTA"
     gene            812..1285
                     /locus_tag="VIN32_01900"
     CDS             812..1285
                     /locus_tag="VIN32_01900"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY8921359.1"
                     /translation="MKLPPFQRLVDDHAAELHRFLIASVGVVDAEDCLQDTFLSALRA
                     YPRLRHGDNLRAWLYTIAQRKATDAVRAHRRHRATDLDGVAEDRLASPPDRSADDELW
                     ASVRALPAKQRAALVHRFVFDLAYRDVGERMGISEEAARQNVSAGLRRLRMEVDR"
     gene            1282..1899
                     /locus_tag="VIN32_01905"
     CDS             1282..1899
                     /locus_tag="VIN32_01905"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020637828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEY8921360.1"
                     /translation="MTTDLEQQIRNAPLPELDRAAPAVALAKRAGKASLLDVAYAELD
                     SPIGELIVFVTPRGLLRVKYADEPIEGVLADVAARVSPRILRAPTRTDETRRQIEGYF
                     GLRRRTFNLPIDWSLVHGFAAGVLRQTARIPFGDVRSYGQVAAKAGSPRAARATGNAL
                     GSNPIPIVVPCHRVLHADGGLGGYSGGLDRKRYLLALEGALPDRD"
     gene            complement(1905..>2453)
                     /locus_tag="VIN32_01910"
     CDS             complement(1905..>2453)
                     /locus_tag="VIN32_01910"
                     /inference="COORDINATES: protein motif:HMM:NF040123.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6518 family protein"
                     /protein_id="HEY8921361.1"
                     /translation="VVMGNAVGPWVAVAFAAGALQGEPRRGAMGGAIALCAGVATYYG
                     AALLTWGDRAPAFIPQLAAVWLVVAAASGVVVGGAGGGWAANGRYRSIGPIALAGALL
                     AEAAYQFVQVEGWRGVDMTRTGFQIAAIDTVLALLVPLALLERGRRRMAYLGSVAVGA
                     AGFLVLAAVDSLIRAVLFALPA"
BASE COUNT          366 a          878 c          844 g          365 t
ORIGIN      
        1 cctcctcaac gacctgctga tgcaggttcg caaggaacgt accggccgct actcgggggg
       61 tgactatttc accctcgact gagccgatgg tcgaggtcgc tccgggcacg atcgtcgtct
      121 tcagtgacat cgggtgcccc tgggcgcacg tttcggtctg gcgcctgcat gacgcccgcc
      181 gccgcctggg actcgagggc cgcgtcgcct tcgaccatcg cgtcttcccg ctcgagcttt
      241 tcaactcgca acccactccc cgcgacgagc tggcggtgga atgggaggcg tgtgcctcac
      301 tcgcccctcg cgccggctgg caggcgtgga gcgcgccgga ttgggcgtgg ccggcgacca
      361 tgctgcctcc gatggaagcc gtccaggcgg ccaaggagca aggcctcggt gcgtccgagg
      421 agcttgaccg gggcctccgc agagccttct ggggcgagag ccgatgtgtc agcctgcgtc
      481 acgtcatcct cgaggtggcg gcggagtgcg atggcctcga cctcgaggcg ctggcggagg
      541 cgttcgattc cggccgtgct cgcacagcgc tcttcgatca gtacgaggtg gcccgcagcg
      601 atgcggtcga cggatcgccg cacctctttc tcgccgacgg caccaacgtc gagaaccccg
      661 gcatcaaggc aacctgggag gaggggagcg acgggttctg gcgcccgacc gtcgtcgaag
      721 acgatccctc tgcctacgac cgactggtga cggcggccgc cgtcacggcc tgaggcctca
      781 cgaaccggtg gcccgccgcg tttcatcgtt cgtgaaactg cccccgttcc agcggctggt
      841 cgacgaccac gctgcggagc tccatcggtt tctgatcgcc agcgtcggtg tcgtcgacgc
      901 ggaggactgc ctccaggaca ccttcctctc ggctcttcgt gcctacccgc ggctgcgcca
      961 tggcgacaac ctgcgggcct ggctctacac catcgcccag cgcaaggcga ccgatgcggt
     1021 ccgagcgcat cggaggcaca gggcaaccga cctggatggc gtcgcggagg atcggctggc
     1081 gagcccgccg gatcggtcag ccgacgatga gctatgggct tccgtgcggg cgctcccggc
     1141 caagcagcgt gccgccctcg tgcaccgctt cgtcttcgac ctggcctatc gcgacgtcgg
     1201 agagcggatg gggatcagtg aggaagccgc tcggcagaac gtcagcgccg gcctgcgccg
     1261 gctacgcatg gaggtggacc gatgacaacc gacctggagc agcagatccg caatgcccca
     1321 ctcccggagc tcgatcgtgc agcgccggca gtggcgcttg ccaagcgtgc gggcaaggcc
     1381 tcgctgcttg atgtggcgta tgcggagctc gactcgccga tcggggagct gatcgtcttc
     1441 gtcacgcctc gcggcctgct ccgggtcaag tacgccgacg agccgatcga gggggtgctg
     1501 gccgatgtgg cggcgcgcgt ctcgccccgc atcctgcgcg ccccgacgcg caccgacgag
     1561 acgcgtcgtc agatcgaggg ctacttcggc cttcgccgcc ggactttcaa cctgccgatc
     1621 gactggtcgc tggtgcacgg cttcgccgcc ggcgtgctgc gccagaccgc gcgcattccg
     1681 ttcggcgacg tgcgcagcta tggccaggtc gcggccaagg caggctcgcc ccgagccgcg
     1741 cgggcgaccg gcaacgccct cggctcgaac ccgatcccga tcgtggtgcc gtgccaccgg
     1801 gtgctgcatg cggatggcgg gttgggcggc tactcgggcg gcctcgaccg caagcgctac
     1861 ctgttggcgc tggagggtgc cctcccggat cgcgactgag gcgatcaggc ggggagtgcg
     1921 aagagcaccg ccctgatcag ggagtcgaca gccgccagga cgaggaagcc cgcggcgccg
     1981 acggccacgc ttcccaggta ggccatgcgg cggcgcccac gctccaggag cgcgaggggc
     2041 acgagcagcg ccaagacggt gtcgatggcc gcgatctgga agccggtgcg cgtcatgtcg
     2101 acgcccctcc agccctcgac ctgtacgaac tggtacgcag cctcggcgag cagcgctccg
     2161 gcaagggcga tcgggccgat cgaccgatac ctgccgttcg ccgcccagcc acccccggcc
     2221 ccgccaacga caacgccgga ggccgcggcc acgaccagcc acaccgccgc gagctgagga
     2281 atgaacgctg gcgcccggtc gccccaggtc agaagggcgg ccccgtagta agtggcaact
     2341 ccggcgcaga gtgcgatcgc tccgcccatg gcgccccgcc tcggttcgcc ctgcagcgct
     2401 ccggctgcga acgccacggc cacccacggg ccgacggcat tgcccatcac cac
//
LOCUS       DATCQR010000057         4283 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_49198, whole genome shotgun sequence.
ACCESSION   DATCQR010000057 DATCQR010000000
VERSION     DATCQR010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4283
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49198"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="VIN32_01915"
     CDS             <1..327
                     /locus_tag="VIN32_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921362.1"
                     /translation="WLGGGAADCPGGPEPAWLACSPFILQAAGDTRKIGAPQLFVAVD
                     PAASLSIPSEPFPQVKVTGHYDDPAAQTCRETQPIPGESARAAPEFILACRQTFVVTG
                     VVPLGP"
     gene            complement(340..1587)
                     /locus_tag="VIN32_01920"
     CDS             complement(340..1587)
                     /locus_tag="VIN32_01920"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8921363.1"
                     /translation="MLPPSTPELERRRSRALGTYFTAAALTSIAYIATFTVASLAAPE
                     ITGSAGSSGWPSAVAVAGTALAASFLSSVMARRGRRAGIVLGISVAVIGGVLAVVAVM
                     AVSLPLLLVSSALVGFGNAAMNLSRYAAADLFPVERRAGALGFVVWGSTIGAVVGPNL
                     VAPAGAVAPTLGLLPLAGGFAMAFVFLLGALAVALLGPRAPIQPHLDEPTRAVGERPS
                     SQRRLLGELVRRPQGRTALVALVTSQLVMTLIMTMTPYHLHAEGHGLETVGLVISAHT
                     FGMFAFSPLSGRLTDRFGANAMVLAGFSVLAAAGLLAAVMPEGGAALSIPLFLLGVGW
                     NLGFVAGSSLLASEAPLGDRARLQGATDAVVWATAAAASFSSGYVVATASYAFLAVIG
                     ASAAVVLAVAITLDRRNSRPMPA"
     gene            1639..2175
                     /locus_tag="VIN32_01925"
     CDS             1639..2175
                     /locus_tag="VIN32_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015138437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8921364.1"
                     /translation="MDLAIPTLETERLTLRPFREADAAALFELSQDPDVVRYVGDRRA
                     PTLQEAWRAVAGWIGHWALRGYGQWAIEERASGQFIGRAGIINPVDWPGAEVGYLLGR
                     EWWGRGYATEAARGAMDWGFREIGFVDLLSLIDPANQASIAVARRLGESLRGETDLMG
                     NRVLVYGITRAKWAAGQT"
     gene            2172..3413
                     /locus_tag="VIN32_01930"
     CDS             2172..3413
                     /locus_tag="VIN32_01930"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8921365.1"
                     /translation="MTRRGKLLGALSEPQFRLLWIGQTTSAFGDGLTFLAIAFAVLHI
                     GGKATDLGLVFAAFFAAYVSFVLVGGVWADRLPRQLVMVAADLVRGVVQVIIGLLLLT
                     GEAHVWQIAIGVAVYGAASAFFQPASTGLVPQTVSAGRLQQANALMSISRNGGSILGP
                     PVSGLIVAFAGTGAVFMIDAATFGISAISLLLLRPVERTAPEVRQPFLAELAGGWREV
                     LARPWIAAALVAFGISNASGAAFFILGPVIADAQLGGAAAWGLVLTGGAIGGVLGGLL
                     ALRIRPRRPLLVGFAVMSLIALQPLSLVGPAPVLLIAGAFLLGFGAGELTNTWWFTML
                     QQHVPEHARSRVSSYDWLVSFIFQPLGFLAVGPLSQAIGPTATLVGAASLILLADITV
                     TLLPSIRSVTWVPEEAATGDT"
     gene            complement(3475..>4283)
                     /locus_tag="VIN32_01935"
     CDS             complement(3475..>4283)
                     /locus_tag="VIN32_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018350611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEY8921366.1"
                     /translation="VVITSLVVVLSLLFSSAAGATSFPRSMASTGDSITRAFNTGFFP
                     YLDNPSASWSTGTSTTVNSQYLRLLKLNAKISGHAYNDAADGAKMANLAGQMQKAVAQ
                     RVQYVTVLMGGNDFCTSSAGTMTSVATFRSQLTTAMNTLTSGLPSVRVQVVSIPNAYH
                     LWELFKDNFLARSVWAAFSVCQSLLANPTSTEAADVARRDAVRQRNIAFNDVLRDTCA
                     LYSQCLFDGYAVFNTVFVASDITGRDYFHPSTSGQAKLASVTWAAGYWPN"
BASE COUNT          679 a         1434 c         1501 g          669 t
ORIGIN      
        1 tggctcggcg gcggcgcggc ggactgccct ggggggccgg agccagcgtg gctggcgtgc
       61 tcgccgttca tccttcaggc cgccggggat acccggaaga taggcgcgcc ccaactgttc
      121 gtggccgtcg acccggcggc gagcttgtca atcccctccg agccgttccc acaggtcaag
      181 gtaaccggcc actacgatga cccggccgcc cagacctgcc gcgagaccca gccgatccca
      241 ggggaatcgg cccgggcagc gccggagttc atcttggcct gccgccagac gttcgtcgtc
      301 acgggagtcg tgccgctcgg gccctagggg ccggaacact caggccggca tcggtcgcga
      361 attgcgccga tccagggtga tggccaccgc cagcaccacc gccgcgctgg cgccgatcac
      421 ggccaggaag gcgtagctcg cggtcgcgac gacgtagccc gaactgaagc tcgccgcggc
      481 ggcggttgcc cagacaacgg catcggttgc cccctgcagg cgcgctcgat ccccgagcgg
      541 cgcctccgag gcgagtagcg agctccccgc cacgaagccg aggttccagc cgacccccag
      601 caggaagagc gggatcgaca gtgccgcccc gccctcgggc atgacggccg cgagcagccc
      661 tgctgcggcg aggaccgaga agccggcaag gaccatcgca ttcgcaccga atcggtccgt
      721 caggcgcccc gatagcggcg agaaggcaaa catgccgaag gtgtgggcgc tgatcacgag
      781 gccgaccgtc tcgagcccgt gaccctcggc gtggaggtgg taaggggtca tggtcatgat
      841 cagggtcatg accagctggc tggtcaccag ggcgacgaga gcggtgcgcc cctgcggtcg
      901 ccggaccagc tcccccagca ggcgacgctg tgagctcggc cgctcaccca ccgcgcgggt
      961 cggctcgtcg aggtgcggtt ggatcggtgc ccggggaccg agcagcgcca ccgccagagc
     1021 gccgagcagg aagacgaacg ccatggcgaa gcctccggcc agcggcagca ggccaagggt
     1081 cggcgcgacg gccccggccg gggccaccag gttcggcccg accaccgcac cgatggtgct
     1141 gccccagacc acgaacccga gtgcgcctgc tcggcgctcg accgggaaga ggtccgccgc
     1201 ggcgtaacgg ctgaggttca tggccgcgtt gccgaagccg accagcgcgg atgagacgag
     1261 gagcagtggc agcgagaccg ccatcacggc gaccactgcc aggacaccgc caatgaccgc
     1321 gacggagatg ccaagcacga tcccggctct acgtccacga cgcgccatca cggacgacag
     1381 gaacgaggct gccagtgcgg tgccggcgac ggcgaccgcg ctcggccagc cgctcgagcc
     1441 cgccgagccg gtgatttccg gcgcggccag cgacgcgacg gtgaaggtcg cgatataggc
     1501 gatgctcgtc agcgccgcgg cggtgaagta tgtgccgagc gcacgggagc ggcggcgctc
     1561 gagctcgggc gtggagggcg gcagcatcgc gcggatgata gcggcgcctg cacgatgcgc
     1621 tggcgcatca tccccagcgt ggatctggcg attccaaccc ttgagaccga gcggctgacc
     1681 ctccggccgt ttcgcgaggc cgatgcggcg gcgctgttcg agctctccca ggaccccgac
     1741 gtggtccgct acgtcggcga tcgcagggcg ccgacccttc aggaagcatg gcgcgcagtc
     1801 gccggctgga tcggccactg ggcgctgcgc ggctacgggc agtgggcgat cgaggagcgg
     1861 gcgagcggcc aattcatcgg tcgcgcaggc atcatcaacc cggtcgactg gcctggtgcg
     1921 gaggtcggct acctgctcgg tcgcgagtgg tggggccgtg gctatgcgac tgaggccgcc
     1981 cgcggggcga tggactgggg cttccgcgag atcggcttcg tagacctgct gagcctgatc
     2041 gacccggcca accaggcctc gatcgcggtc gccaggcgcc ttggtgagtc actccgcggc
     2101 gagaccgatc tgatgggcaa ccgggtgctg gtctacggca tcacccgagc caaatgggcg
     2161 gcaggccaga catgacccgc cgaggaaagc tgctcggagc gctcagcgag ccgcagttcc
     2221 gactcctgtg gatcggtcaa accacctcgg cattcggaga cggcctgacc ttcctcgcca
     2281 ttgccttcgc cgtactgcac atcggtggca aggcgacgga ccttggactc gtgttcgcgg
     2341 cgttcttcgc ggcgtacgtc tcgtttgtcc tggttggcgg cgtatgggcc gatcggctcc
     2401 cacgccagct cgtgatggtt gccgccgacc tggtgcgcgg cgtcgtgcag gtgatcatcg
     2461 ggctcctgct cctgaccggc gaggcgcatg tctggcagat cgcgattggt gtcgcggtct
     2521 acggtgcggc cagcgcattc ttccaacccg cgagcacggg actcgtcccc cagacagtca
     2581 gcgcaggacg gctccagcag gccaacgcgc tgatgagcat ctcgcgcaat gggggctcga
     2641 tcctggggcc acccgtctcc gggctgatcg tggccttcgc cggtaccggc gcggtcttca
     2701 tgatcgatgc cgcgacattc ggcatcagcg ccatctcgct gctcctgctg aggccggtcg
     2761 aacgaaccgc gccggaggtg cgtcagccgt tcctcgccga gctggccggc ggatggcgcg
     2821 aagtcctcgc ccgcccgtgg atcgccgccg ccctggtcgc gttcggaatc agcaacgctt
     2881 cgggagcggc gttcttcatc ctgggtccgg tgatcgccga cgctcagctc ggtggggccg
     2941 cagcctgggg tctggttctc accggtgggg cgatcggcgg cgtcctcgga ggcctcctgg
     3001 ctctgcgaat ccgtcctcgg cgtccattgc tggtgggctt cgcggtcatg tccctcatcg
     3061 cgttgcaacc gctctctctc gtgggccccg cgccggtcct gctcatcgcg ggcgcgttcc
     3121 tgctggggtt tggcgccgga gagctgacca acacgtggtg gttcacgatg ctccagcaac
     3181 acgtgcccga acacgcgcga tcacgggtga gcagctacga ctggctggtt tctttcatct
     3241 tccagccgct cggcttcctt gcggtggggc cgctgagcca ggccatcgga cccacggcca
     3301 ccctggtcgg ggccgcctcg ttgatcctgc tggccgacat cacggtaacc ctgctgccca
     3361 gcatccggag tgtcacctgg gtcccggagg aggcagcaac cggagacaca tgaccttcgg
     3421 gcccatcgga ctcatgccca gcgctgcgcc aggccccgcg ttaccggcta aggactagtt
     3481 cggccagtac cccgccgccc acgtcaccga tgccagcttg gcctggccgc ttgtcgatgg
     3541 gtggaagtag tcgcgacccg tgatatcgct ggccacaaag acggtgttga agacggcgta
     3601 tccgtcgaac aggcattggc tgtacagggc gcaggtgtcg cgcaacacat cattgaaggc
     3661 aatgttccgc tggcggacgg cgtcgcgcct ggcgacgtcg gcggcctcgg tcgacgtcgg
     3721 gttggcgagc agggactggc agacgctgaa tgccgcccag acgctgcgcg ccaggaagtt
     3781 gtccttgaac agctcccaca ggtgataggc attgggaatg ctgacgacct gcacacggac
     3841 ggaggggagc ccggacgtca gcgtgttcat ggccgtcgtc agctggctgc ggaaagtggc
     3901 cacgctggtc atcgtgcccg ccgaggaggt gcagaagtca ttcccgccca tcaggactgt
     3961 gacgtactgc acgcgctgtg cgaccgcctt ctgcatctgg ccggccaggt tggccatctt
     4021 tgccccatcg gcggcatcgt tgtaggcatg gccgctgatc ttggcattca gcttcaacag
     4081 ccggagatac tggctgttga ccgtggtcga ggtgccggtc gaccaggagg cggaagggtt
     4141 gtcgaggtac gggaagaagc cggtattgaa cgcgcgggtg atcgaatcac cggtcgatgc
     4201 catggacctc ggaaagcttg ttgcaccggc agccgacgag aagagcagcg agaggacgac
     4261 gacgaggctg gtaatgacga ccg
//
LOCUS       DATCQR010000058         3836 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_95549, whole genome shotgun sequence.
ACCESSION   DATCQR010000058 DATCQR010000000
VERSION     DATCQR010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3836
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95549"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..681)
                     /locus_tag="VIN32_01940"
     CDS             complement(<1..681)
                     /locus_tag="VIN32_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016331676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HEY8921367.1"
                     /translation="MDHQELRRRFAALTTAHLADACIRAQVPVRCGPAALGPVVPGSR
                     LAGAALPVRHAGSVDVFLEAFEGAAPGDVLVVDNGGRLDESCVGDLVVLEAQTAALDG
                     ILIWGLHRDTADIRAIGLTVFSLGSIPTGPQRLDLRSKDALEAATVGDWTVGREDLVL
                     ADDDGVILVPAASAADVFTLAETIRATEGSQANLMRGGASLRSQVRFADYLVERRKTP
                     SLSFRDHLR"
     gene            complement(693..1058)
                     /locus_tag="VIN32_01945"
     CDS             complement(693..1058)
                     /locus_tag="VIN32_01945"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY8921368.1"
                     /translation="MPTYASVAGLEPNQIWNGVVARPLHGERITVGILEFEPLTPVPE
                     HRHDNEQLGLVLKGSITMVIGGERRNLQAGGMYSIGSGVPHSGESGPDGATVMDAFSP
                     ARSDWNDSTRLAVAAGRWP"
     gene            complement(1130..2047)
                     /locus_tag="VIN32_01950"
     CDS             complement(1130..2047)
                     /locus_tag="VIN32_01950"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY8921369.1"
                     /translation="MVLPEWLEFRPRLFPSCNFAWLAGPRPVLVDSGFGSDLEATLGL
                     LPGEPALVFNTHWHSDHVGGNAALAERFRMPIAASAAEGSRVNDGDLESFGSDWLDQP
                     VDPYRVDRLLEPGELLETGAVSLQVVPAGGHSLGQVALFEERARVLIAGDALLARDVP
                     WINPFLDGAEALETAIATVERIGLLDARVAVAGHGEVIEDVGSCVKRSLDRLRLWRHD
                     PARMAFHGSRRIFGFALMIHGGFARSELLPYLLARRWVQDFGPVAGLAAEEFAERLVA
                     DLEGSGAARWKHDRLVSTVPHSKVLAVGS"
     gene            complement(2057..2605)
                     /locus_tag="VIN32_01955"
     CDS             complement(2057..2605)
                     /locus_tag="VIN32_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012650993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HEY8921370.1"
                     /translation="MGSPGPATISLVAAGSAFGVRRSLPYVLGIIIGTVAVLVAVATG
                     ITAALLAVPAIGSVLIWISAAYILWLAYHIATAPPLSEPATTSGAFSLAGGALLGVAN
                     PKAWLAIAAVFASAHVAAAATADATAKVAVLIAMIIVICTTWLVAGTAIAPLLREPRR
                     ARLVNAALAVGLVAATTLAVLH"
     gene            2704..3561
                     /locus_tag="VIN32_01960"
     CDS             2704..3561
                     /locus_tag="VIN32_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009981964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEY8921371.1"
                     /translation="MPQTLGGMDVDRLERSCSLGSDSIRFGPSANGIERAEVHLAACA
                     FEPHRHDTYAIGITTAGVQTFRYRGSRRVCLPGQLHVLHPDELHDGAAGTDEGFGYRI
                     LYIPPDLVRDALGGAELPFVADPVQEPIPATRRIASLLIDIDEPINELTSAEIAAAVA
                     DSLVSLSGKARHRQARIDLTAVQLVREYLTVHACEPTPASTLEQVAGIDRFTIARHFR
                     WAFGTSPDRYRTLRRLAVARAAIEGGRSLARTAAEAGFADQSHMTRQFKRTYGLTPGR
                     WMALTTAAS"
BASE COUNT          645 a         1349 c         1298 g          544 t
ORIGIN      
        1 gcgcaggtgg tcgcggaaac tcagggaggg cgtcttccgg cgctcaacca ggtagtcggc
       61 gaagcggacc tggctgcgca gggacgcgcc tcctcgcatg aggttggcct ggctcccctc
      121 ggtggcgcgg atcgtctcgg caagcgtgaa gacgtccgcg gcgcttgcgg ccggcacaag
      181 aatcacgccg tcgtcgtcag ccaggaccag gtcctcgcga cccaccgtcc aatcgccgac
      241 ggtcgctgcc tccaacgcat cttttgagcg caggtcgagg cgttgagggc cagtcggaat
      301 cgatccaagg ctgaagaccg tcagtccgat cgctcggatg tcagcggtgt ctcgatgcag
      361 cccccagatc aagattccat cgagtgctgc cgtctgggcc tccagcacca cgaggtcgcc
      421 gacgcagctc tcgtcgagcc gcccgccgtt gtcgaccacc aacacgtcgc cgggagcggc
      481 cccttcgaac gcctccagaa agacatcgac gctgccagca tgtcgaaccg gaagcgcagc
      541 cccggccagg cggctgccgg gcacaactgg gcccagggcc gccggcccac agcgcaccgg
      601 gacctgagcc cggatgcaag catccgccag gtgggcggtg gtcagcgccg cgaagcgccg
      661 ccgcagctcc tggtgatcca tcctcgtccc cgctacggcc agcgccctgc cgcgacggcc
      721 aggcgtgtcg agtcattcca gtcggatcgc gccggcgaga acgcatccat gacggtcgcg
      781 ccatcgggcc ccgactcacc agagtgagga acgccgctcc cgatggagta catgccgccg
      841 gcctgcaggt tgcggcgctc gccgccgatg accatcgtga tcgaaccctt gaggacgagt
      901 cccaattgct cgttgtcgtg gcggtgctcg ggcaccggtg taaggggttc gaactcgagg
      961 atcccgaccg tgattctctc gccgtgcagg ggacgggcca cgaccccatt ccagatctga
     1021 tttggctcga gcccggcgac cgaagcatag gtgggcattt ctcagacctt aggagcgttg
     1081 acggcgaacg tcttgaacga tcctgcagca gcgcgtcccg ggcgcggcgc tagcttccga
     1141 cggcgagaac cttgctgtga gggacggtcg agaccagccg gtcatgcttc cagcgtgcgg
     1201 cgccggaacc ctcgaggtcg gcaacgagcc gctccgcgaa ctcctccgca gccaggcccg
     1261 ccacgggtcc gaagtcttgg acccagcgcc gggccaggag gtacggcagt agctcggagc
     1321 gagcgaatcc gccgtggatc atgagggcga agccgaagat gcgccggctg ccgtggaagg
     1381 ccatccgggc gggatcgtgg cgccagagcc ggagtcgatc cagcgatctc ttcacgcagc
     1441 tcccgacatc ttcgatgacc tcgccatggc cggccaccgc gacccgggcg tcgaggaggc
     1501 cgatccgctc gacggtggcg atcgccgtct ccagcgcctc ggccccgtct aggaagggat
     1561 tgatccaggg cacgtcacgg gcgagcaggg cgtcccccgc gatcaggacc cgggcgcgct
     1621 cctcgaaaag ggcgacctgt cccagcgagt gcccaccggc aggcaccacc tgcaggctca
     1681 cggcgccggt ctccaacaac tcgcccggtt ccaacagccg gtccacgcga taggggtcga
     1741 ccggctgatc cagccagtcc gagccgaatg actcgaggtc gccgtcgttg acccgcgatc
     1801 cctcggcggc cgaggcggcg atcggcatcc tgaagcgctc cgccagtgcc gcgttgccgc
     1861 cgacgtggtc cgagtgccag tgcgtgttga agacgagcgc cggctcgccc ggcagcaggc
     1921 cgagggtcgc ctccaggtcg ctgccgaagc cgctgtcgac caggaccggc cggggaccgg
     1981 ccagccacgc gaagttgcag ctgggaaaca ggcgcggccg gaactcaagc cattcgggga
     2041 ggaccacgtc tggacttcag tgaagcaccg ccagcgtggt cgcggcgacc agccccacgg
     2101 ccagcgcggc gttgacgagg cgggctcgac ggggttcacg gagcagcggc gcgatcgccg
     2161 tgcccgcgac caaccaggtc gtgcagatga cgatgatcat cgcgatcagc accgcgacct
     2221 tggcggtggc gtccgcagtc gcggcagcgg cgacgtgggc gctcgcgaag accgccgcga
     2281 tcgccagcca cgccttcggg ttggccacac cgaggagcgc tcccccggct agcgagaagg
     2341 cgccggaggt cgtggccggc tccgagagcg ggggcgcggt cgcgatgtga taggccagcc
     2401 agaggatgta cgccgccgag atccagatca ggacggagcc gatcgcgggg accgccagca
     2461 gtgcggcggt gatcccggtc gcgacggcga cgaggacggc gaccgtgccg atgatgatcc
     2521 cgagcacgta gggcagggat cgcctcacgc cgaaggccga gccggcggcg accaggctga
     2581 tcgtcgcggg accgggactg cccatgatcg cgagcgaaat cagcaccagc ggcccaaccg
     2641 atccccaccg gatcgcaact actgcaagcg cctcaggcat cgcggctgct gtaccttcgt
     2701 gccatgccgc agacgctagg tggcatggat gtcgatcgtc ttgaacggtc ctgcagcctg
     2761 ggttccgact cgatccggtt cggaccgagt gccaacggca tcgagcgcgc ggaggtgcac
     2821 ctggccgcct gcgccttcga gccgcaccgg cacgacacct acgcgatcgg catcaccacg
     2881 gccggcgtcc agacttttcg ctaccgcggc tcccgccgcg tctgcctgcc gggccaactg
     2941 cacgtgctgc atcccgacga gctgcatgac ggcgcggcgg ggaccgatga aggcttcggc
     3001 taccggatcc tctacatccc gcccgacctg gtccgtgacg cgctcggcgg cgccgagctg
     3061 ccgttcgtcg ccgacccggt gcaggagccg ataccggcga ctcgccggat cgcctcattg
     3121 ctgatcgaca tcgacgagcc gatcaacgag ctcaccagcg ccgagatcgc cgccgccgtc
     3181 gccgacagcc tggtgtcact cagtggcaaa gccaggcacc gccaagccag gatcgaccta
     3241 acggccgtcc agctcgtgcg cgaatacctc accgtccacg cctgcgagcc gaccccagcc
     3301 tcgactcttg aacaggtcgc ggggatcgat cgcttcacca tcgcccgcca cttccgctgg
     3361 gcgttcggga caagccccga ccgctatcgg acgctgcgcc gtctggcggt cgcgcgagct
     3421 gcgatcgagg gcgggcggtc gctggcccgt accgccgccg aggccgggtt cgccgaccaa
     3481 agccacatga cgcgccagtt caagcgaacc tacggcttga cgccgggccg ctggatggca
     3541 ctcacgaccg ccgcttcgta accgtcagat gacgcggcag taagcgctgt tcggattctg
     3601 gacagggtgg gtggcggtcc cacgtggcct cgaaccagac gccgatcccg taggtgtcgt
     3661 gccggtgcgg atcgaacccg cgcgtggaga tgtagacctc tgcgcgctcg acgccgtaag
     3721 cgctcggtcc gaacctgatc gagtccgcgc gtcggccgca ggatcgttca agacggtcag
     3781 catcggcgcc tcgtaccgtc ggcggcatga gtcaacggta tcgcggcggc aatgcc
//
LOCUS       DATCQR010000059         3016 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_219575, whole genome shotgun sequence.
ACCESSION   DATCQR010000059 DATCQR010000000
VERSION     DATCQR010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3016
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219575"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(68..517)
                     /locus_tag="VIN32_01965"
     CDS             complement(68..517)
                     /locus_tag="VIN32_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003801682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family peroxiredoxin"
                     /protein_id="HEY8921372.1"
                     /translation="MAAVRTATVTWQGPLATGDGTVTAGSSGLFTDLPVSWGSRTEAP
                     DGRTSPEELLAAAHAACFAMAFSADLGRAGYTPEEMHVEAEVTFDKLEAGWTVTRSAL
                     TVLGHVHGITPEEFDRIAEGTRDGCPISRALAGNVELSVDATLETDG"
     gene            627..1190
                     /locus_tag="VIN32_01970"
     CDS             627..1190
                     /locus_tag="VIN32_01970"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEY8921373.1"
                     /translation="MVYLRPAERDDIDLFVRWFADAETTRYLATRAPFSKAMEERWFD
                     SMLEQQGRRSYHFVICLLADDRAIGTAGFHHVNQEDGNASFGISIGEKAEWSKGYGTD
                     ALRAICDFGFGQLRLERIELDVYEPNRRAQRSYEKAGFVNEGTLRHAHFSDGRFHDVV
                     RMSLLREEWAAQPRSRPWDEPITDHGA"
     gene            1191..2180
                     /locus_tag="VIN32_01975"
     CDS             1191..2180
                     /locus_tag="VIN32_01975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921374.1"
                     /translation="MFELRRDPVTGWWSAIVTDRTFEHASFVVQATPIEGTHCRHCDD
                     DPTPSAEAAEETAIRRVTLRQDAFHRIDTGQERTGQLSVEQAMAASGSWEILVAPREH
                     HERLADAPPRLAVNLLRTLRDTMREMAAEPRPEDEPRYIQAVQSYGMQAGSRSSHLNM
                     ELYSIPQVPHWVAEEIGGGARYVIKTRSCVWCSLARSEEESGERLVLAEERFVVFAPA
                     ASRSAFELMIVPRAHAADFTTLDDDDIAAATATLQRTLGALDALGDPPYNLFLHTAPV
                     GERLDETFHWHWEVHPRLRVIAGLERATALAVNPAAPEYAAGLLRGHLAHVSV"
     gene            2252..2794
                     /locus_tag="VIN32_01980"
     CDS             2252..2794
                     /locus_tag="VIN32_01980"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY8921375.1"
                     /translation="MTVPRDGSDARKAEFDRIFTEYSAPIYNYALRMVGDPDRAADIT
                     QDTFIKAYRKLDTLTDPSSTRSWLYRIATNTAIDEMRRRRNVVRLDADEDRTVEQAEG
                     GPGPETQVLSGLMDERISRALLHLKPNHRQCLILSDVEDMSAVQIGEVMGMSNGAVRV
                     LLCRARGEMRRRLAAEGLVR"
     gene            2791..>3016
                     /locus_tag="VIN32_01985"
     CDS             2791..>3016
                     /locus_tag="VIN32_01985"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEY8921376.1"
                     /translation="MRLRGNHVDELISASLTGDLTDAERVELDSHLGRCETCRNTLAA
                     FTAERRILSGLPMAEPPRDLGARVRSGIESG"
BASE COUNT          488 a         1055 c         1036 g          437 t
ORIGIN      
        1 ggtcaccgcg gccaggacgc gaccgacggc ccccagggcg tcccgctcgg aggtcggaac
       61 ctcctgttca gccatccgtt tcgagcgttg cgtcgaccga cagttccacg ttcccggcca
      121 gtgcgcgcga gatcgggcag ccatccctgg tgccttccgc gatgcggtcg aactcttcgg
      181 gcgtgatccc gtgcacgtga ccgaggacgg tgagggcgga gcgcgtgacc gtccagccgg
      241 cctcgagctt gtcgaaggtc acctcggcct cgacgtgcat ctcctccggc gtgtagcccg
      301 cccggcccag gtcggccgag aacgccatgg caaagcaggc agcatgcgcg gcggcgagga
      361 gctcctcggg cgaggtgcgg ccgtcgggcg cctcggtgcg ggacccccac gagacgggta
      421 ggtccgtgaa caagccggac gagccggcag tgacagtgcc gtcgccggtg gcgagcgggc
      481 cttgccatgt gacagtagcg gtgcggacgg cggccatcgg atcggtcctc cttgtatcct
      541 gcggggactt cagcctaccg cacgcaggac cgatgtcgag cgccgagcca agccctccac
      601 gacccatccc cagcattcgc ggcgagatgg tctacctgcg ccccgccgag cgggacgaca
      661 tcgacctgtt cgtacgctgg tttgccgatg ccgagaccac ccgctacctg gccacgcgcg
      721 ccccgttcag caaggcgatg gaggagcgct ggttcgactc gatgctcgaa cagcagggca
      781 ggcgaagcta ccacttcgtc atctgcctcc tggccgacga tcgggcaatc ggtaccgcgg
      841 gcttccacca cgtgaaccag gaggacggca acgcctcgtt cggcatcagc atcggcgaga
      901 aggcggagtg gtccaagggc tacgggaccg atgcgctgcg cgccatctgc gacttcggct
      961 tcggccagct gcgcctggaa cggatcgagc tggacgtcta cgagccgaac cggcgcgccc
     1021 aacgctcgta cgagaaggcc ggcttcgtga acgaagggac gctgcggcac gcgcatttct
     1081 ccgacgggcg cttccacgat gtcgtgcgga tgtcgctcct tcgcgaggaa tgggctgcac
     1141 agccgcgctc gcggccgtgg gacgagccga tcaccgacca cggagcctag gtgttcgagc
     1201 tgcgtcgcga cccggtcacc ggctggtggt cggcgatcgt cacggacagg accttcgagc
     1261 acgcatcgtt cgtcgtgcag gcaaccccga ttgaagggac ccactgccgg cactgtgacg
     1321 acgacccaac gccctccgca gaggccgcgg aggagaccgc catccgccgc gtcacgctgc
     1381 gccaggacgc cttccaccgg atcgacaccg gccaggagcg cacgggccag ctctccgtcg
     1441 agcaggcgat ggcggcaagt ggctcgtggg agatcctggt cgcgccgcgc gaacatcacg
     1501 agcgcctggc ggatgccccg ccgcgactgg cggtgaacct gctgcggacc ctccgcgaca
     1561 cgatgcgcga gatggccgcc gaaccgcgac ccgaagacga gcctcgctac atccaggccg
     1621 tgcagagcta cggcatgcag gccggctcgc gcagcagcca cctgaacatg gagctgtact
     1681 cgatcccgca ggtcccccac tgggtggccg aggagatcgg tgggggcgct cggtacgtca
     1741 tcaagacccg ctcctgcgtg tggtgcagcc tcgcccggag cgaggaggag tcgggcgagc
     1801 gactcgtcct ggccgaggag cgcttcgtgg tcttcgcgcc tgccgccagc cgctcggcgt
     1861 ttgagctgat gatcgtgccg cgggcgcatg ccgccgactt caccacgctc gacgacgacg
     1921 acatcgcggc ggccaccgcc accctgcagc ggaccctcgg tgcgctcgac gccctgggcg
     1981 acccgcccta caacctcttc ctgcacaccg cccctgtcgg agagcggctg gacgagacct
     2041 tccactggca ctgggaggtc catccgcggc tgcgggtaat cgccggactg gagcgcgcga
     2101 cggcacttgc ggtcaatccc gcggcgccgg aatacgcggc cggcctcctg cgcgggcacc
     2161 tcgcccacgt gagcgtgtaa caggtgccag cccagcggcg tccactgcac cgatggcgct
     2221 tgctccacgg ctcgcatggc gttccgcgac ggtgactgtg ccgcgggatg gctccgacgc
     2281 gcgcaaggcc gagttcgatc ggatcttcac cgagtactcg gcgcccatct acaactacgc
     2341 gctgcggatg gtgggtgacc cggatcgggc cgccgacatc acgcaggaca ccttcatcaa
     2401 ggcctaccgc aagctggaca ccctgaccga tccctcctcg acgcgttcct ggctctaccg
     2461 gatcgcgacc aataccgcga tcgacgagat gcggcgccga cggaacgtcg tccggctgga
     2521 tgccgacgag gatcgcaccg tcgagcaggc ggagggcggt cccggcccgg agacccaggt
     2581 gctgagcggc ctcatggacg agcgcatcag tcgcgccctg ctgcatctga agccaaacca
     2641 ccgacagtgc ctgatcctgt cggacgtgga ggacatgagc gcggtgcaga tcggcgaggt
     2701 gatggggatg tcgaatggcg cggtccgagt gctgctgtgc cgcgcccggg gtgagatgcg
     2761 ccgccggctg gcggcggagg gcctggtgcg atgagactcc gcggcaacca tgtcgacgag
     2821 ctgatcagcg catcgctgac cggcgacctg accgatgcag agcgcgtcga gctggactcg
     2881 cacctcggtc gctgcgagac gtgccgcaac accctcgccg ccttcaccgc cgagcgcagg
     2941 atcctgtcag ggctgccgat ggcggagcca cctcgcgacc tcggggcgcg ggttcgcagc
     3001 gggatcgagt ccggcc
//
LOCUS       DATCQR010000060         2337 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_65066, whole genome shotgun sequence.
ACCESSION   DATCQR010000060 DATCQR010000000
VERSION     DATCQR010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2337
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65066"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..456
                     /locus_tag="VIN32_01990"
     CDS             <1..456
                     /locus_tag="VIN32_01990"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HEY8921377.1"
                     /translation="NRLPSTFGGVTVRGSGSDEDILRGAGADLAELLMALTQGDNRNA
                     LAAQLGKHIFGIPRVIAKINDPLRAEAYRTLGLETICRTVILSDALVVAATRGAEATE
                     GAVEPPTAEPRQGMPPAGSRAAAKAKAAIEEAATETSATEEPAVGGEAS"
     gene            458..1117
                     /locus_tag="VIN32_01995"
     CDS             458..1117
                     /locus_tag="VIN32_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEY8921378.1"
                     /translation="MYVVVIGGGNVGYYLTKELLAAGHEVVMIEKDPGRARTIADELG
                     SIVVPNDGCEGRYQAEAGMARADVVAAVTGDDEDNLVACQVAKMKFNVPRAIARVNNP
                     SNEALFRRLAIDDTVSPTRSILALIEHEIPIHELLHLAELEGGEVQIVEAQLDANSPV
                     IGKVLRDLELPEGSAVAVVVRDEHAIVVRADTTLRAGDKLLAVTSAEHEAELRSLLIG
                     E"
     gene            1142..2143
                     /locus_tag="VIN32_02000"
     CDS             1142..2143
                     /locus_tag="VIN32_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007914993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEY8921379.1"
                     /translation="MTDATTRPQDQRGAQFLASLQQAVSGAVVGGELPLRLLATALLA
                     GGHALVEDVPGVGKTLLARAFARALGLTFARVQGTPDLLPTDVTGSSILDQGQFRFIP
                     GPVFTNVLLVDEINRATPRTQSALLEAMQERQVSAEGQTRPLPDPFLVLATQNPIELE
                     GTFALPEAQLDRFLLRISMGYPDEDAERRIARRHQASAEPLDDVPTVIQPDEALELRA
                     IARTVHVSDDVERYMVALVRATREHADVRLGASPRSSVALYRVGQASALLAGRSFVLP
                     DDVRTVAHAVLAHRLLLEVDRELRGATVTGVVQEVIDSVPVPMADEELIAGNRLLTA"
     gene            2146..>2337
                     /locus_tag="VIN32_02005"
     CDS             2146..>2337
                     /locus_tag="VIN32_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921380.1"
                     /translation="MGDWIALAGAMAVVAVVADAPGVLLVAVLTLGYGSLTTVWTRYG
                     MRRVEYSRRLGTGRAVAGDS"
BASE COUNT          376 a          798 c          828 g          335 t
ORIGIN      
        1 aaccggctgc cgagcacctt cggcggcgtc acggtgcggg gcagcggcag cgacgaggac
       61 atcctccgtg gcgcgggggc cgatctcgcc gagctgctga tggcgctcac ccagggcgac
      121 aaccgcaacg cgctggccgc ccagctgggg aagcacatct ttggcatccc gcgtgtgatc
      181 gcgaagatca acgaccccct gcgtgccgag gcctatcgca ccctcggtct cgagacgatc
      241 tgccgcaccg tgatcctctc cgacgcgctg gtcgtcgccg caaccagggg agcggaggcg
      301 accgaggggg ccgtggagcc gccgaccgcc gagccgcgtc aggggatgcc gccggccgga
      361 tcccgggctg cggccaaggc caaggccgcg atcgaggaag cggcgaccga gacttccgcg
      421 accgaggaac ccgcggtcgg cggcgaggcc agctgacatg tacgtggtcg tcatcggcgg
      481 cgggaacgtc ggctactacc tgaccaagga gctgctggcc gccggccacg aggtggtcat
      541 gatcgagaag gaccccggac gggcacgcac catcgccgac gagctcggca gcatcgtggt
      601 ccccaacgac ggctgcgagg ggcggtacca ggccgaggcc gggatggccc gcgcggacgt
      661 ggtggccgcc gtgaccggcg atgacgagga caaccttgtc gcctgccagg tggccaagat
      721 gaagttcaac gtcccgcgcg cgatcgcccg ggtcaacaac ccgagcaatg aggcgctctt
      781 ccgacgcctt gcgatcgacg acacggtcag ccccacgcgg agcatcctcg ccctgatcga
      841 acacgagata ccgatccacg aactcctgca tctcgcggag ctcgaaggcg gcgaggtgca
      901 gatcgtcgag gcccagctgg acgccaactc gccagtgatc ggcaaggtgc tccgcgacct
      961 ggagctcccc gaggggagcg cggtggcggt ggtggtccgc gacgagcacg ccatcgtggt
     1021 ccgcgccgac accaccctgc gggccggtga caagctgctg gcggtcacct cggccgagca
     1081 cgaagcggag ctgcgcagcc tgctgatcgg cgaatgagcc ccgaccagaa tcgtccctga
     1141 catgactgac gccaccacgc gcccccagga ccagcgcggt gcccaattcc tggcatcgtt
     1201 gcagcaggct gtctccggtg ccgtcgtcgg gggcgaactg ccgctgcgct tgctggccac
     1261 cgccctgctg gcaggcggtc atgcactggt ggaggatgtg cccggcgtcg gcaagacgct
     1321 cctggcacgg gcgttcgcgc gcgcactggg cctcaccttc gctcgcgtcc agggcacgcc
     1381 cgacctgctc ccgaccgatg tcaccgggtc gagcatcctg gaccaggggc agttccgctt
     1441 cataccaggc cccgtgttca caaatgtgct cctggtggac gagatcaacc gagcaacccc
     1501 acggacacaa tcggcgctgc tggaggcgat gcaggaacgc caggtcagcg ccgagggtca
     1561 gaccaggccg ctgccggacc cattcctggt gctggccacc cagaacccga tcgagctgga
     1621 aggcacgttc gcgctgcccg aggcgcagct cgaccgattc ctgctgcgta tttcaatggg
     1681 ctatcccgat gaggacgcgg agcgccgcat cgcgcgccgc caccaggcct cggccgagcc
     1741 actcgacgat gtgccgaccg ttattcagcc ggacgaggcg ctggagctgc gtgccatcgc
     1801 ccgtaccgtc cacgtctcgg acgacgtgga gcgctacatg gttgcgctgg tgcgcgccac
     1861 gcgcgagcac gcagatgtgc gcctcggcgc ctcgccacgc agcagtgtcg cgctgtaccg
     1921 cgtgggacag gcatcggcgc tgctcgccgg gcgcagtttc gtgctgcccg atgacgtccg
     1981 cacggtggcg cacgcggtgc tcgcgcatcg gctcctgctc gaggtcgacc gggagctgcg
     2041 tggggctacc gtgacgggcg tcgtccagga ggtcatcgac agcgtgccgg ttccaatggc
     2101 agatgaggag ctgatcgccg gaaaccggct cttgacggcg taggaatggg cgattggatc
     2161 gctctcgccg gtgcgatggc ggtggtggcc gtggtggcgg acgcgccagg cgtcctgctg
     2221 gtggctgtgc tgacgctcgg ttacggatca ctcacgaccg tctggactcg atacgggatg
     2281 cggcgggtgg agtacagccg gcggctgggg accggtcgcg cggtcgccgg cgacagc
//
LOCUS       DATCQR010000061         3767 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_157781, whole genome shotgun sequence.
ACCESSION   DATCQR010000061 DATCQR010000000
VERSION     DATCQR010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3767
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157781"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..753)
                     /locus_tag="VIN32_02010"
     CDS             complement(<1..753)
                     /locus_tag="VIN32_02010"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY8921381.1"
                     /translation="MDTISMTDISAPQLGQLQAFIEVARRGTISRAAEALFVSQPALT
                     ARIQGLERTLGSSLFARGQHGSHLTEAGRALLPHAERALTAVERGREAVAEVASGSGG
                     RLTIGAAPAVSTYVLPTMLHRFQADHPGVHLSVRSGHSEDVLEMVLREEVDLGLMRPI
                     QHAEVISSLLYEDELVLVVHRRHPFASSGPILMERMATEHLILFDRTSSYHELTSAVF
                     RQAGIAPRGYLEVDNIDAAKRMVEQRLGIALLP"
     gene            995..3295
                     /gene="aceA"
                     /locus_tag="VIN32_02015"
     CDS             995..3295
                     /gene="aceA"
                     /locus_tag="VIN32_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005148330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase ICL2"
                     /protein_id="HEY8921382.1"
                     /translation="MSAPAALAAPAPDLSAPALQDELETTRRWMEGERFARTTRLYSP
                     RQVVEQRGMIDTNYPVARNAADRFYARLRELFDQRRSITTYGPYSPGQAIAMKLAGIE
                     AIYLGGWATSAKGSMDEDPGPDLASYPLGQVPNEAAGLVRALLTADRNQRFARARMTD
                     VQRAAAPEIDFEPWIIADADTGHGGDAHVRNLIRRFVEAGVPGYHIEDQKPGAKKCGH
                     QGGKVLVPSDEQIKRLNAARFQLDVMGVAGIIVARTDAESASLLEGGVDERDQPFILG
                     VTNVEVPAYRVGYLAVMRRLHSAGVTDINGHLLYAIPEAEYALADAWLEEVGLTDVID
                     KAARAHQEDANSTPEETLDAVADRFLEAWQERAALKTLGQAVADLMTFRADEGFPVEI
                     GVEEWLEFAGLASWSATRARASELGLQATWDPEHARTPEGYYQVQGGIGYAIAKSLAA
                     APFADLLWMETKTADLADAREFAEAVHAIYPDQMLAYNLSPSFNWDTTGMTDEEMRGF
                     PAELGKLGFVFNFITYGGHQIDGLAAEEFATALREDGMVALARLQRRLRLLESPYRTP
                     QTLVGGPRADAALLAASGQTATTKAMGPGSTQVQHLVQTEIPPKLLAGWLGRWAKHHG
                     LDATLSVSLRPHTAGSEVLELSVLDPSGEKVANIVFATIADRRGRSMLSVRDQNTFDT
                     TLRRKRLMTLLHLFLIHRYRASAVHYLTPTEDNRRQGQRMLELGIFKSVNDEVGEIIV
                     ADVDAAVIRALVDPARSELEHLITGS"
     gene            complement(3317..3547)
                     /locus_tag="VIN32_02020"
     CDS             complement(3317..3547)
                     /locus_tag="VIN32_02020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921383.1"
                     /translation="MTKTSAGSSPYPDDEDINLDEIPEFDFSNAVRGTYAEWAKRAKG
                     HFYEVTDEEDAKRSAAERRPKGLLKKLSRPKP"
BASE COUNT          637 a         1276 c         1239 g          615 t
ORIGIN      
        1 gggcaggagg gcgatcccca ggcgctgctc gaccatccgc ttggcggcgt cgatgttgtc
       61 cacctcgagg tagccgcgcg gcgcgatccc cgcctgccgg aaaacggcgc tcgtcagctc
      121 gtggtaggaa ctggtgcggt cgaacaggat caggtgctcg gtcgccatcc gctccatcag
      181 gatcgggccg ctcgacgcga acggatgccg acggtgcacg accagcacga gctcgtcctc
      241 atagagaagc gaggagatga cctcggcgtg ctggatgggt cgcatgaggc cgaggtcgac
      301 ctcctcgcgc agcaccatct cgagtacatc ctccgagtgg ccgctgcgca ccgacaggtg
      361 cacgcccgga tggtccgcct ggaaccgatg cagcatggtc ggaaggacat aggtgctcac
      421 cgccggcgcc gcaccgatgg tcagtcgccc gccactcccg ctcgccactt cggccactgc
      481 ctcgcggccg cgctccactg cggtgagcgc ccgctcggcg tgcggcagca aggcgcgccc
      541 ggcctcggtc aggtggctgc cgtgctggcc gcgagcaaaa agcgagctac cgagtgtgcg
      601 ctccagcccc tggatgcgcg ccgtgagcgc tggctggctt acgaaaaggg cctccgccgc
      661 acggctgatc gtgccgcgac gagcaacctc gatgaaggcc tggagttgtc cgagctgcgg
      721 agccgagata tcggtcatgc tgatggtatc catcaataga atcgccttga ctgatactac
      781 cccacccgcc agggtggtgc gatgatgact gtgacgctac cccagacacg ctcttcggcc
      841 cgccaggccg agtgggctaa cgcgtggctg cagtggagac ccgagctccc tgaggagccc
      901 aggcgcctga ccgtggccga gctggcagag tgcctctgcc cggacctctg cgaccgcgac
      961 cactcgaacg agtagcaacg acgcgtctat ggtgatgtca gcgccggcgg cgctggcggc
     1021 gccggctccg gacctgtccg cccccgctct ccaggacgag ctggagacaa cccgccgctg
     1081 gatggagggg gagcgcttcg cgcgcaccac ccgcctctac tcaccgcgcc aggtcgtcga
     1141 acagcgcggg atgatcgaca ccaactatcc ggtcgcccgc aacgcggcgg atcggttcta
     1201 cgcccggctg cgagagctgt tcgaccagcg gcgctcgatc accacctacg gtccctactc
     1261 gccagggcag gcgatcgcca tgaagctggc cgggatcgag gcgatatatc tcggaggctg
     1321 ggccacatcg gccaaggggt ccatggacga ggaccctggc cccgaccttg ccagctatcc
     1381 gctgggccag gtgcccaacg aggccgccgg cctggtccgg gcactcctca ctgctgaccg
     1441 caaccagcgc ttcgcccgcg cccggatgac cgatgtgcaa cgtgcggctg cgccggagat
     1501 cgacttcgaa ccctggatca tcgccgacgc cgataccggc catggtggcg acgcgcatgt
     1561 ccgcaacctc atccgccgct tcgtggaggc cggtgtgccc ggctaccaca tcgaggacca
     1621 gaaaccgggc gccaagaagt gtggccacca gggcggcaag gtgctggtcc caagcgacga
     1681 gcagatcaag cgcctcaacg ccgcccgctt ccagctcgac gtgatgggcg tcgcgggcat
     1741 catcgtggcc cggaccgatg ccgagtccgc aagcctgctc gagggtggag tggacgagcg
     1801 cgaccagccc ttcatcctgg gggtgacgaa cgtcgaggtg ccggcctacc gagtgggcta
     1861 cctggcggtc atgcgccggc tccacagcgc tggcgtgacc gatatcaacg ggcacctgct
     1921 ctacgcaatc cccgaggccg agtacgcatt ggccgatgca tggctcgagg aggtcggact
     1981 gaccgacgtc atcgataagg cagctcgagc ccaccaagaa gatgcgaatt ccaccccgga
     2041 ggagacactc gatgccgtgg ccgaccgctt cctggaggcc tggcaggagc gcgccgcact
     2101 gaagacactc ggccaggctg tcgcggacct catgacattc cgggccgatg aggggttccc
     2161 tgtcgagata ggtgtcgagg agtggttgga gttcgcgggc ctcgcgtcat ggagcgccac
     2221 ccgggcgagg gcctccgagc tggggctcca ggccacatgg gatcccgagc atgcgaggac
     2281 acctgagggc tactaccagg tccagggagg catcgggtac gcaatcgcga agtcgctcgc
     2341 tgcggcgccc ttcgcggacc tgctctggat ggaaaccaag acggctgacc tggccgatgc
     2401 gcgcgagttc gccgaggcag tccacgcgat ctacccggac cagatgttgg cctacaacct
     2461 gtcgccctcg ttcaactggg acacgaccgg gatgaccgat gaagagatgc gcggattccc
     2521 tgccgaattg gggaagctcg ggttcgtctt caacttcatc acctacggcg gccaccagat
     2581 cgatgggctg gcggccgagg agttcgcgac cgcgctgcgt gaggacggga tggtcgccct
     2641 tgcgcgtctg cagcggagac tgcgccttct tgagtccccc tatcggacgc cgcagacgtt
     2701 ggtcggcggg ccgcgggcgg atgccgcgct gctggccgcg tccggacaga cggcaaccac
     2761 caaggccatg ggacccggct cgacccaggt tcagcacctg gtccagacgg agatcccgcc
     2821 aaagctgctg gccgggtggc ttgggcgctg ggccaagcat catggcctgg acgccacact
     2881 cagcgtctcg ttacgccccc acacggcggg ctccgaggtg cttgagttga gcgtcctgga
     2941 cccgtcgggg gagaaggttg ccaacatcgt cttcgccacc atcgcggatc gccgcggccg
     3001 ctcgatgctg tccgtgcgcg accagaacac gttcgacacg acgctgcgac gcaagcgctt
     3061 gatgacgctg ttgcacctgt tcctgatcca ccgctaccga gcttccgcgg tccactatct
     3121 gaccccgacc gaggacaatc gccgccaggg ccagcggatg ctcgagctgg gaatcttcaa
     3181 gtcggtcaac gacgaggtag gcgagatcat cgtggccgac gtggacgccg cggttatccg
     3241 tgcccttgtc gaccccgccc gatccgagct ggagcacctg atcaccgggt cctgaggctc
     3301 ccgctcgcag cccccgctag ggtttcgggc gactcagttt cttgagaagc cccttcggtc
     3361 ggcgctcggc cgcggagcgc ttggcgtcct cttcatcggt cacctcgtag aagtgaccct
     3421 tggcgcgctt ggcccactcg gcgtacgttc ctcggaccgc gttcgagaag tcgaactccg
     3481 ggatctcgtc gaggttgatg tcctcgtcgt cagggtaagg tgatgaaccg gcgcttgtct
     3541 tggtcatggc gttttcgctc ccgctgcgtg gcgggccgtg cagagatgac acggattcta
     3601 tctcctcgat aggaatgggc cactgccaag agccggcccg attcggctcg gccaatggtc
     3661 aggaaccgat cctcgccctg tgaattcacg acgtcatgga tcgtccgcga caacctcgac
     3721 atcctgcgtc gctacctgcc cgctgccgcc gtcgacctgg tctacct
//
LOCUS       DATCQR010000062        15019 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_65110, whole genome shotgun sequence.
ACCESSION   DATCQR010000062 DATCQR010000000
VERSION     DATCQR010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 15019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15019
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65110"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="VIN32_02025"
     CDS             <1..253
                     /locus_tag="VIN32_02025"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:NF020720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEY8921384.1"
                     /translation="EELAAAGRLGTAILPVGSLLHLPELRLDADAAGRFVHGSAVQVP
                     GATGSGRHAVFGDGELLGVGMLQDGLLEPRTVIAANGAA"
     gene            250..1191
                     /gene="ribF"
                     /locus_tag="VIN32_02030"
     CDS             250..1191
                     /gene="ribF"
                     /locus_tag="VIN32_02030"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF018299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HEY8921385.1"
                     /translation="MSGEPRRLGLADLPSIGRAVITMGVFDGVHLGHRAILETTRDAA
                     AGGGATSVALVFDPPPDELLRPGTQVARLAPLTTNLTRIEALVDRAIPVRFDEQLREL
                     SAEAFLEALAPALSLDGLTMSSRSAFGRDRGGTAARMQELGAERGFSLRLVEPVEVAG
                     SVVSSTRIRASITDGDVDAARAMGVVPYLEGVVVTGDRRGRELGFPTANLRFGYRPAM
                     PALGIYAGRVTEAGPSVASGHPALISIGTRPTFHEGTEVLAEVHLLDFDGDLYGVRLG
                     VQLLARLRDEQRFADASALVAQMQRDALRGRAVLGVA"
     gene            1273..1542
                     /gene="rpsO"
                     /locus_tag="VIN32_02035"
     CDS             1273..1542
                     /gene="rpsO"
                     /locus_tag="VIN32_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011437932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEY8921386.1"
                     /translation="MPLAGDTKSAIMDDFATHDGDTGSPEVQIALLTERIKSLTEHLR
                     TYQNDNHSRRGLLKLVGQRRRLLAYLVRNDVDRYRAVIGRLGLRR"
     gene            1769..4375
                     /locus_tag="VIN32_02040"
     CDS             1769..4375
                     /locus_tag="VIN32_02040"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: protein motif:HMM:NF008805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEY8921387.1"
                     /translation="MKVEADIGGQPFSFEAGKLAEQADGAVLVRYGDTMLLATAVSAE
                     PREGIDFFPMTIDFEERMYAAGKIPGGFIKRESRPSEAAILAMRLTDRPIRPLFPKGY
                     KQEVQIVLTVLSTDQENDPDILAINGASAALSISSIPFLGPIGAVRVGYIDGAFVTNP
                     TNTQLNDSDLDLVVAGTREAVMMVEAGAKILSESVMADAIEYAHKELQHSIDLQEKLT
                     ATAGKPKKLPFLGPKADSIVKLGKALGESDSEFVVLDLETTAMKPENGYIVDLAALRV
                     KGGKVVDRFESLVNPGRSIVGHQIHGLSDDDVDKAPTAAEVLTKFAAWVGDAPVVAHN
                     VAFDLPFVLRHMPHDAQWQPSAVYDTLELAYQLYPDAGSYKLADLVRFVFGRDHGAAH
                     RAMPDAEAAAELFIDLTSGLAERLEKIRTDIAAEIRRGKTDYNRAAQGDRLEDIRRQH
                     GIGSGLMDVLTKATSRELVLSENIRIDGRDTTTIRPISVEVGILPRTHGSGLFQRGQT
                     QALSIATLGPSSDVQRLDTISPETEKRYMHHYNFPPYSVGEARPMRGPGRREIGHGAL
                     AERALLPVLPSADEFPYAMRVVSEVVSSNGSTSMASTCGSTLALMDAGVPIKAPVAGA
                     AMGLISDTATGRHAVLTDISGKEDAYGDMDFKVAGTAEGVTALQMDIKVGGIPMSVMR
                     DALEQARVARLFILDKMTAVISASRSELSPYAPRITTIKIPIDKIRDVIGSGGKVIRQ
                     IVAETGTQINVEDDGTIQIASVSEENAKKAIAWIEGLTKDVEVGKEYLGKVTRIMNFG
                     AFVEIMPGKEGLVHISQLADYRVAQVEDVVAVGDELMVVVTEIDRMGRVNLSRRAAME
                     RHMAKAGVSEES"
     gene            4381..5049
                     /locus_tag="VIN32_02045"
     CDS             4381..5049
                     /locus_tag="VIN32_02045"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEY8921388.1"
                     /translation="MGADALLSAIAAEARACTRCPLHIGRTQAVPGEGNPLSDVLLVG
                     EGPGAREDATGRPFVGPAGQLLDELLQSIGWAREHVFIANVVKCRPPGNRDPESGEIN
                     ACRPYLDRQEAALDPAVIVTLGRHSLRRYLPDARIGEAHGRLRRSGSRFVFPMYHPAA
                     ALHQSSLRETLFADMRGLPAALLAAREAIEAERTESVQAISPDPAPPAPTAEIVDAEQ
                     MTLF"
     gene            5057..6721
                     /locus_tag="VIN32_02050"
     CDS             5057..6721
                     /locus_tag="VIN32_02050"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018132499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEY8921389.1"
                     /translation="MPDTLRIIPLGGVGEVGKNITVIEAAGEILVVDCGLAFPEQEML
                     GIDLVIPDVTYLRENRDRVKAILLTHGHEDHTGGLPYVLPQIPGTPIYTSRLTAGLVT
                     GKLKEHKLLGQTRLEVVEPAVEFRIGAFAITPFRVNHSIPDGMGFAIRTPLGTVVHTG
                     DFKFDHTPPDGRRADLGLIAEIGNHGVEFLLSDSTRAEREGYTLSEKTVGDSLNQLVR
                     EASGRVIVATFASNIGRVQQVLDAAWASGRKMVALGRSMEQNIAIALELGYLDPRDGT
                     LTGKDGLQRLSKDRLVVMTTGSQGEPMSGLTRMSNRDHRNITIEPGDTVIISASPIPG
                     NEEAVAKTIDNLFKEGAMVHYEPLVHCHVSGHGSREELKLMLGLVKPRHFIPVHGEYR
                     MLVQHGLLAEGAGVPVDGIFVLENGQVVEFDGTRARLAGSVPAGAVLVDGIAAIDGVD
                     GVVLRDRRALASDGVVMVALTVDRATGQAVSGPDLVGRGFLSDPDDPVMAAACKHLTE
                     ALLQPVADAHAAEVGYLKTKIRDTLSRYFFERTKRRPMILPIVMEV"
     gene            6729..9086
                     /locus_tag="VIN32_02055"
     CDS             6729..9086
                     /locus_tag="VIN32_02055"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HEY8921390.1"
                     /translation="MTRRRTNGSRRRGSSRRRKATTTVTISGRVAREVLALVLVVAAI
                     VSTIALFAPDAGLIVKPWHDALSFLLGWGIAFAPVLLAGFAFMLWLKTMPAERWMAAT
                     GAAVMSIGLLGIFHLTGGNGPEGVNRGEGGGAIGFAASAALSGAIGAAGAWIVLGLML
                     VVGLLLYFNMTVGDLVAAWLRRRDERVELAELEARRASADPRVRPESAAATAAPGGRG
                     LLQRMRTALGGGAEPDDEPLILRRARPAEGTVPVPRAHDVPFPIGEIEPLVAVPVTVD
                     ELESEETAHLAEVEHHEVADAALEATIHSWDLPQVSFLDDAPASSAAQLDLTAKGQRI
                     RDTLAHFGIGVKVARIQEGPVVTQYALDVEPGIKLSRIEGLADNLALDLKARSIRIQA
                     PIPGEPYVGIEIPNSAFDLVTLKEVLSSPNFDEASGRSKLAFALGQDVAGQPFSADLS
                     RMPHLLIAGATGSGKSVCVNAIICSLLMRASPSEVKLILIDPKRVEMAQYKGIPHLLT
                     EVIVDNDKSVNALKWTVGTMESRYHEFAQRGVRNIAGYNEALKPGEPRMPYIVIVIDE
                     LADLMMVSAYEVEATITRIAQLARATGIHLVVATQRPSVQVITGLIKANIPSRIAFAM
                     TSGVDSRTILDTVGAEDLLGRGDMLYQPIDAPRAVRLQGVLVTDHEIETVTRHWRGQG
                     GPQYRPEITAPKRDGKAGGRPGEEDDEGDALLSAAVDIVRRSDKASASLLQRRLRIGY
                     ARAARILDQMEDRGIVGPADGSRFREVLVTPDGWGGEIGPDEDGE"
     gene            9083..9820
                     /locus_tag="VIN32_02060"
     CDS             9083..9820
                     /locus_tag="VIN32_02060"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY8921391.1"
                     /translation="MTEREPTKLGTVLRTAREARFIDLARVERDTKIRVRYLSALESG
                     NYGDLPGSLYTRGFLRNYGLYLGLDPEYLVDLYRLESGSAIERPSMPSARRPMAARQG
                     RSLVVSSGAVVAAILTLLVVAFVVYLATEFVTFARTPDLRITDPATDVLAWHGTEYTI
                     RGVTEPSSTITTDGLRQNPSATADADGAFSVLIGLVPGANVITLVANDPLTGRDSAAV
                     RRTITVVTASPTPSQLPSAGAAGGSTP"
     gene            9860..10348
                     /locus_tag="VIN32_02065"
     CDS             9860..10348
                     /locus_tag="VIN32_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011056636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HEY8921392.1"
                     /translation="MADSSFDVVSDFDQQELVNALDQVRREVATRYDFKGAKVSLELG
                     KDEITLHADDDFRAGSVKDLLQSKAVRRGLSLKVFDWGKLEPAAGSTVRQRIGLHRGL
                     TSEQAKEIAKLVREQFPKVKPAIQGDAVRVSGKSKDELQGVINHLRGLDYAVALQFIN
                     YR"
     gene            10345..10851
                     /locus_tag="VIN32_02070"
     CDS             10345..10851
                     /locus_tag="VIN32_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HEY8921393.1"
                     /translation="MNPPSEADLARAGARLGTALRARTWSLATAESSTGGLIGQAITM
                     TPGASDYYAGGVICYSNRAKEVVLGVPHALIAAHGAVSEEVAAALAQGAAHRFETDLG
                     VSVTGIAGPDGGSEQKPLGTHYVSVVRRGHPARVEHHLFGHDRTGNRAAAALAVLELA
                     LDEVSAAA"
     gene            10851..12281
                     /gene="murC"
                     /locus_tag="VIN32_02075"
     CDS             10851..12281
                     /gene="murC"
                     /locus_tag="VIN32_02075"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEY8921394.1"
                     /translation="MRAGERIHLIGIAGSGAAGVALLLHHAGAQIDGCDADTPSPYTP
                     PLDAAGIRVISGHNPSHLAGVERVVITPALRANPDLPELAAARAAGLPIVTWQAMLGE
                     LMAAEGRIGLAVTGTHGKSTTTALLGHLLESAGLDPTVEVGAFIPAWGATVRRGAGVP
                     FLIEADEFGDNFLNYHPAGAIVTNVEMDHPDYFADREAVLASMERFVRGMGEDSRLGG
                     RLLLTTAADTGSQALLERLGDWQGRTMPYGPGGEIEAHDVAYAGGGATFTLFEHPFES
                     PLAGEHNVLNATAALVMARELGAPLDALSEGLREFRGAGRRMELIADTAGVIVYDDYG
                     HHPTEVRATLAAMRQKVGDRRLWAVFEPHMYSRTALFLDEFAHAFTDADEVVIVDIFA
                     SRDTDASVRATSAEALADAIERTSAVSTMAGGDVDAATAYVAQHLSPGDAVLVMGAGK
                     SYQIAQGLKAALEGGVLTQSRSLPDQ"
     gene            12278..12652
                     /locus_tag="VIN32_02080"
     CDS             12278..12652
                     /locus_tag="VIN32_02080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921395.1"
                     /translation="MSGSRSVFLGALLALGVAILQEALVRLVEASGARDQPAVILVLL
                     LAAFLGLLTAVAVRALRLPGAVAPTVLVIGWIVVPPLLKAIPAPVTQTFASDAALTAP
                     DAVALAVAVIAAAVTLGVQRER"
     gene            12716..14590
                     /locus_tag="VIN32_02085"
     CDS             12716..14590
                     /locus_tag="VIN32_02085"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8921396.1"
                     /translation="MPDPESPVSGPDPTGSPAAQPDSVTRGFLFSDLRGYTQFVETRG
                     ATTAAALLVRYRELARSAIARFGGAEIKTEGDSFYVVFRSVSSAVRCGLAITADAAAA
                     SIEHPYEPIQVGVGIHAGETVESDGGYVGSAVNIAARICAQAASGEVLVSETVRALTR
                     SLLTVRFEERGRRQLKGIADPIALFAVLDAGPGSIPAARGRPRWLRGRRLLVATLGGA
                     ALLAGAAAFGWGALHPAGLPPGPWRIGLDMPLTGDTASRGQPMQNAAQLAIRDANKAG
                     GILGSMLTLKSYDDAGAPPNGQDPDQGAANATSMIGDPRTIAMIGPSGSSVARAILPL
                     TNQAGLFECSPANTSPGLTKPRDGALDLRSAHPDEINYVRVAPSDDIQALALASFASR
                     DLAADKVALVIDDTGDGREIADGFQQEYTKLGGVVIRRALNAGADPMSVLGPSADGTP
                     APQVVFFGGYTQTGAAQLRGAMSKAGLGAVPFLSWDGILDGSAADTGSFIQLTGSAAI
                     GSYLAHASLGPHKANFADAYRAAYGEEPDEYSAAAYACGEVIVASLRAIAANSPSTDG
                     LRRALRTYAVDPSHQYATVLGTVGFDSNGDSTQQFVTFYRVDAGDWVIDKQQDFGPAR
                     "
BASE COUNT         2427 a         5176 c         5143 g         2273 t
ORIGIN      
        1 cgaggaactg gcggccgcgg ggcggctggg cacggcgatc ctgccggtgg gttcgctcct
       61 gcacctgccc gagctgcgcc tggatgcgga tgccgcaggt cgcttcgtgc atggatcggc
      121 cgtgcaggtg ccgggggcaa cgggtagcgg gcgccacgcg gtgttcggcg acggtgagct
      181 tctcggcgtc ggcatgctgc aggacggcct gctcgagcca cgaacggtga ttgcagcaaa
      241 cggtgccgcg tgagtggcga gccacggcgc ctggggctgg ctgacctgcc gtcgatcggc
      301 cgcgccgtca tcacgatggg cgtcttcgat ggcgtgcacc ttggccaccg ggccatcctg
      361 gagacgaccc gagacgctgc cgccggagga ggtgcgacca gcgtggcact cgtcttcgac
      421 ccgcctcccg acgagttgct ccgacctggc acccaggttg ctcgcctggc gcccctgacc
      481 acaaacctca cgcggatcga agcgctggtc gacagggcca tcccggtccg cttcgacgaa
      541 cagctccgtg agctcagcgc cgaggcattc ctcgaggccc tggcgccggc cctctcactc
      601 gacgggctga cgatgtcctc ccggagcgcg ttcgggcgcg accggggagg gacagcggca
      661 cgcatgcagg agctcggcgc cgaacgtggc ttttccctcc gactggtcga gccagtcgag
      721 gttgcgggat cggtcgtgtc ctcgacccgc atccgagcgt cgatcaccga cggagacgtg
      781 gatgcggcgc gagcaatggg cgtcgtgccg tacctggagg gggtcgtcgt gacgggagat
      841 cgacgcggcc gcgagctggg ctttccgacc gccaacctgc gattcggcta ccggccagcc
      901 atgcccgcgc tcggcatcta cgcgggccgc gtgacggagg ctgggccaag cgtcgcaagc
      961 ggacatccgg cgctgatcag catcggcacc cggccgacct tccacgaggg tacggaggtg
     1021 ctggcggagg tgcacctgct cgacttcgac ggcgacctgt acggggtgcg gctcggcgtc
     1081 cagctgctgg cgcgcctgcg cgacgagcag cgattcgcag atgccagcgc cctcgtggcg
     1141 cagatgcagc gcgacgcgct gcgcgggcga gccgtgcttg gagtggcctg atcggaggct
     1201 ggccgatcgg cgtccgaggc ctcggtctgg tacgatgcag ggcgaaaagt ggaaagaaaa
     1261 gtggaggaat acgtgccgct cgctggagac acgaagagcg caatcatgga cgacttcgcc
     1321 acgcacgatg gcgataccgg atcccccgag gtgcagatcg cactgctcac cgagcgcatc
     1381 aagtcgctga ccgagcacct gcgcacctac cagaacgaca accattcgcg ccgcgggctg
     1441 ctcaagctcg tcggtcagcg ccgaaggctg ctcgcctacc tcgtccgcaa cgacgtggat
     1501 cgctaccgcg cggtgatcgg tcgcctgggg ctgcgccgct aatcacgaac tcgtcgcaac
     1561 cacgacccag gagtcatatc gactcggggt cgcctgcttc cggcccagca tcccggccgg
     1621 acccatggat gccatcggca tccgccaatc atcagccatc cgcgcccgca agggccgccg
     1681 cccgccatct cgatctgatc gggattcgtg tgcgggccga gcgccgggtc gcgtggcaag
     1741 agcaagactt ctggaggaat cagtggcaat gaaagtagag gccgacatcg ggggccagcc
     1801 cttctcgttc gaggcgggca agctcgccga gcaggccgat ggcgcggtgc tcgtgcgtta
     1861 cggcgacacc atgctcctgg cgacggcggt ctccgccgag ccgcgcgagg ggatcgactt
     1921 cttccccatg acgatcgact tcgaggagcg gatgtatgcc gcgggcaaga tccccggcgg
     1981 cttcatcaag cgggaatcgc ggccgtcgga ggcagccatc ctggcgatgc gcctgaccga
     2041 ccgacccatc cggcccctct ttcccaaggg ctacaagcag gaggtgcaga tcgtcctgac
     2101 ggtcctctcg accgatcagg agaacgatcc cgacatcctg gccatcaacg gtgcctccgc
     2161 ggcactctcc atcagcagca tcccgttcct ggggcccatc ggggcggtgc gagtgggcta
     2221 catcgacgga gccttcgtaa ccaacccgac caacacccag ctgaacgaca gcgacctcga
     2281 cctggtcgtg gcaggcaccc gtgaggccgt gatgatggtc gaggctggag caaagatcct
     2341 gtccgagtcg gtcatggccg atgccatcga gtacgcccac aaggagctgc agcacagcat
     2401 cgacctgcag gagaagctga cggccacagc cggcaagccg aagaagctcc cgttcctcgg
     2461 tccgaaggcc gattcgatcg tgaagctcgg caaggcgctc ggcgagagcg acagcgagtt
     2521 cgtcgtgctc gacctcgaga cgaccgccat gaagccagag aacggctata tcgtcgacct
     2581 ggccgccctc cgggtgaagg gcgggaaagt cgtcgatcgc ttcgagtcgc tcgtgaatcc
     2641 cggtcgctcg atcgtgggtc accagatcca cggcctgagc gacgacgatg tcgacaaggc
     2701 cccgaccgcg gcggaggtgc tcaccaagtt cgcggcatgg gtcggcgacg cgccggtggt
     2761 cgctcataac gtcgccttcg acctgccgtt cgtgctgcgc cacatgcccc acgatgccca
     2821 gtggcagccg agcgccgtct acgacacgct cgagcttgcc taccagctct accccgatgc
     2881 gggctcctac aagctcgccg acctggtgcg cttcgtcttc ggacgtgacc acggagccgc
     2941 gcaccgcgcg atgcccgatg ccgaggcggc ggccgagctg ttcatcgacc tgaccagcgg
     3001 cctggccgag cggctcgaga agatccgcac cgacatcgcc gccgagatcc ggcgcggcaa
     3061 gaccgactac aaccgagccg cccagggcga tcggttggag gacattcgac gccagcacgg
     3121 catcggctcc gggctgatgg acgtcctgac caaggccacc tcccgcgagc tcgtgctgtc
     3181 ggagaacatc cgcatcgacg ggcgcgatac caccaccatc cggccgatca gcgtggaggt
     3241 cgggatcctg ccccgcaccc atggctccgg gctcttccag cggggccaga cccaggccct
     3301 ctcgatcgcg accctcggac cctcgtccga cgtgcagcgc ctcgacacga tctcgccgga
     3361 gaccgagaag cgctacatgc accactacaa cttcccgccc tactcggtcg gcgaggcgcg
     3421 gccgatgcgt ggaccgggcc ggcgcgagat cggccacggt gcgctcgccg agcgggccct
     3481 gctgccggtc ctgccgtccg ccgacgagtt cccgtacgcg atgcgggtgg tcagtgaggt
     3541 ggtctcctcg aacggctcga cctcgatggc cagcacctgc ggctcgaccc tggcgctgat
     3601 ggatgccggc gtgccgatca aggctcccgt cgctggggcg gccatgggcc tcatctccga
     3661 taccgccacc ggtcggcacg cggtgctgac cgacatcagc ggcaaggaag atgcgtacgg
     3721 cgacatggac ttcaaggtgg cgggcaccgc cgaaggtgtg accgccctgc agatggacat
     3781 caaggtcggc ggcatcccga tgtcggtgat gcgcgacgcc ctggagcagg cgcgcgttgc
     3841 gcgtctcttc atcctcgaca agatgaccgc ggtcatcagc gccagccgca gtgagctgtc
     3901 gccgtacgcg ccgcgcatca cgaccatcaa gatcccgatc gacaagatcc gggacgtgat
     3961 cggctccggc ggcaaggtga tccgccagat cgtggccgag accggcaccc agatcaacgt
     4021 cgaggacgac gggacgatcc agatcgcgtc ggtctccgag gagaatgcca agaaggcgat
     4081 cgcctggatc gagggcctga ccaaggacgt cgaggtcggc aaggagtacc tcggcaaggt
     4141 gacccgcatc atgaacttcg gcgccttcgt cgagatcatg cccggcaagg aggggctggt
     4201 gcacatcagc cagctggccg attatcgggt ggcgcaggtc gaggacgtgg tcgccgtcgg
     4261 cgacgagctg atggtggtgg tgaccgagat tgaccgcatg ggccgcgtca acctgagccg
     4321 ccgtgcggcc atggagcggc acatggccaa ggcaggcgtc agcgaagagt cctgacgccg
     4381 atgggggccg acgcgctcct ctccgcgatc gcggccgagg ccagagcctg cactcgctgc
     4441 cccctccaca tcggccggac ccaggctgtt ccgggcgagg gcaacccgct gagcgacgtg
     4501 ctgctggtag gggaggggcc gggcgcccgt gaggatgcga ccgggcgccc cttcgtgggg
     4561 ccggcgggac agctcctcga cgagctgctg cagtcgatcg gctgggcgcg cgagcacgtc
     4621 ttcatcgcca acgtggtgaa gtgccgccca ccgggcaacc gcgacccgga gtcgggggag
     4681 atcaacgcct gccgtcccta cctcgaccgc caggaggcgg cgctcgatcc cgcggtgatc
     4741 gtcaccctgg ggcgccactc gctgcgtcgc tacctgccgg atgcgcgcat cggagaggcg
     4801 cacggccgcc tgcggcgatc gggcagccgc ttcgttttcc cgatgtacca tcctgctgcg
     4861 gcgctgcacc agtcctcgct gcgggagacc ctcttcgcag acatgcgtgg cctgccggct
     4921 gcgctgcttg ccgcacgcga ggcgatcgag gccgagcgga cggaatcggt ccaggcgatc
     4981 tcgcccgatc cagcgccacc ggcgccgacg gctgagatcg tcgacgccga acagatgacc
     5041 ctcttctagg agcaccatgc cagacaccct tcgcatcatt ccccttggcg gggtgggcga
     5101 ggtcggcaag aacatcaccg tcatcgaggc ggccggggag atcctggtcg tcgactgcgg
     5161 acttgccttc cccgagcagg agatgctcgg gatcgacctc gtcatcccgg acgtgaccta
     5221 cctgcgcgag aatcgggatc gcgtcaaggc catcctgctg acccatggcc acgaggacca
     5281 caccggcggg ttgccgtacg tcctgccgca gatccccggc acgccgatct acaccagccg
     5341 cctcaccgcc ggcctggtga ccggcaagct gaaggagcac aagctcctcg gccagacccg
     5401 cctggaggtc gtcgagccgg ccgtggagtt ccgcatcggc gccttcgcca tcaccccctt
     5461 ccgagtgaac cattcgatcc ccgatggaat gggcttcgcg atcaggactc cgctcgggac
     5521 ggtggtgcac accggcgact tcaagttcga ccacaccccg cccgacggcc gtcgcgccga
     5581 cctcggcctc atcgccgaga tcggcaacca cggcgtcgaa ttcctgctct ccgactcgac
     5641 gcgcgccgag cgcgagggct acacgctctc ggagaagacg gtgggggact cgctcaacca
     5701 gctcgtcaga gaggcgagcg ggcgcgtcat cgtcgccacc ttcgcgagca acatcggtcg
     5761 cgtgcagcag gtgctcgacg ccgcctgggc aagcggccgc aagatggtgg cgctggggcg
     5821 cagcatggag cagaacatcg cgatcgccct ggagctcggc tacctcgacc cgcgcgacgg
     5881 gacgctgacc ggcaaggacg ggctccagcg cctgtccaag gaccggctgg tggtgatgac
     5941 caccggctcc cagggtgagc ctatgtcggg actgacccgg atgagcaacc gagaccatcg
     6001 gaacatcacg atcgagccgg gcgatacggt gatcatctcg gcctcgccga tcccgggcaa
     6061 cgaagaggcc gtggccaaga cgatcgacaa cctcttcaag gagggggcga tggtccacta
     6121 cgagccactg gtccattgcc acgtgtcggg ccacggctcg cgggaggagc tgaagctgat
     6181 gctcggcctg gtcaagcccc ggcacttcat cccggtccat ggcgagtacc gcatgctcgt
     6241 gcagcacggg ctgctggccg agggggcagg tgtccccgtc gacggcatct tcgtgctgga
     6301 gaatgggcag gtggtcgagt tcgacgggac acgggcgcgg ctcgccggct ccgttccggc
     6361 aggggcggtg ctggtcgacg gcatcgcagc catcgacggg gttgacggcg tggtcctgcg
     6421 agatcgccgc gccctcgcct cggatggcgt ggtgatggtc gccctgaccg tcgaccgcgc
     6481 caccgggcag gccgtctcgg gaccggacct ggtcggccgc ggcttcctct ccgaccccga
     6541 cgatccggtc atggcggccg cctgcaagca cctgaccgag gcgctgctgc aaccggtcgc
     6601 cgacgcgcat gccgccgagg tcggctacct gaagacgaag atccgcgaca ccctctcgcg
     6661 ctacttcttc gagcgcacca agcgacggcc gatgatcctg ccgatcgtga tggaggtctg
     6721 accgaccctt gaccaggcga cgcacgaacg gctcgcggcg gcgcggcagc tcacgtcgcc
     6781 gcaaggccac gaccacggtc accatcagcg ggcgggttgc ccgcgaggtg ctcgccctcg
     6841 tcctggtcgt ggccgcgatc gtcagcacca tcgcgctctt cgcgcccgac gccggtctga
     6901 tcgtgaagcc ctggcatgac gcgctctcat tcctgctcgg atgggggatc gccttcgcgc
     6961 cggtgctttt ggccggcttc gcattcatgc tctggctcaa gacgatgccg gccgagcgct
     7021 ggatggccgc caccggcgct gcggtcatgt cgatcggcct gctcgggatc ttccacctga
     7081 ccggcggcaa tggccccgaa ggggtgaacc ggggcgaggg tgggggagcc atcggcttcg
     7141 cggcctcggc ggcattgagc ggcgcgatcg gtgcggccgg cgcctggatc gtgctgggcc
     7201 tgatgctcgt ggtcggcctg ctgctctact tcaacatgac cgtcggtgac ctggtggcgg
     7261 cctggctgcg gcgacgcgac gagcgggtcg agctggcgga gctcgaggcg cgccgagctt
     7321 cagccgatcc acgcgtgcgg cccgagtccg ccgcggcgac cgccgctccc ggagggcgtg
     7381 ggctgctgca aaggatgcgc accgccctgg gcgggggagc cgagcccgac gacgagcccc
     7441 tcatcctgcg tcgcgccaga ccagccgagg gcaccgtacc ggtgccgcgg gcgcacgacg
     7501 tcccgttccc gatcggtgag atcgagcccc tcgtggccgt gcccgtcacc gtcgacgagc
     7561 tggagagcga ggagaccgct cacctggccg aggtcgagca ccacgaggtg gccgacgccg
     7621 cgctggaggc gacgatccac agctgggacc tcccgcaggt gtccttcctg gacgatgcgc
     7681 cggccagcag cgccgcccag ctcgacctaa ccgccaaggg ccagcgcatc cgcgacaccc
     7741 tcgcccactt cgggatcggg gtcaaggtgg cccgcatcca ggaggggccg gtcgtcaccc
     7801 agtacgcgct ggacgtggag ccggggatca agctgtcgcg gatcgagggg ctggccgaca
     7861 acctggcgct cgacctgaag gcgcgaagca tccggatcca ggcgccgatt cccggtgagc
     7921 cgtacgtcgg gatcgagatc ccaaacagcg ccttcgacct cgtgaccctc aaggaggtgc
     7981 tcagctcccc gaacttcgac gaggcgtccg gccgcagcaa gctcgccttc gcgcttgggc
     8041 aggacgtcgc cggccagccc ttcagcgccg acctgtcgcg gatgccccac ctgctgatcg
     8101 caggggcgac aggatcgggg aagagcgtct gcgtcaacgc catcatctgc tcactgctca
     8161 tgcgcgcctc gccgtccgag gtgaagctga tcctgatcga ccccaagcgg gtcgagatgg
     8221 cgcagtacaa gggcatcccg cacctcctga ccgaggtgat cgtcgacaac gacaagtcag
     8281 tcaacgccct gaagtggacg gtcgggacca tggagagccg ctaccacgag ttcgcccagc
     8341 gcggcgtgcg caacatcgcc ggctacaacg aggcgctgaa gccgggcgag ccgcggatgc
     8401 cgtacatcgt gatcgtgatc gacgagctgg ccgacctgat gatggtcagc gcctacgagg
     8461 tggaggcgac catcacgcgc atcgcgcagc tggcgcgtgc caccgggatc cacctggtgg
     8521 tcgccaccca gcgtccatcg gtgcaggtca tcaccggcct gatcaaggcc aacatcccat
     8581 cgcgcatcgc gtttgcgatg accagcgggg tggactcgcg cacgatcctg gacacggtcg
     8641 gcgccgagga cctgctggga cgcggcgaca tgctctacca gcctatcgat gcgccccgcg
     8701 cggtccgcct gcagggcgtc ctggtcaccg atcacgagat cgagacagtc acgcgacact
     8761 ggcgtgggca gggcggcccg cagtaccgac ccgagatcac ggcccccaag cgtgatggca
     8821 aggccggcgg gcgtcccggc gaagaggacg atgagggaga cgccctcctt tcggccgcgg
     8881 tcgacatcgt ccgccgctcg gacaaggcgt cggcctcgct gctccagcgc cggctgcgga
     8941 tcggctacgc acgcgcggcc aggatcctcg accagatgga ggaccgcggc atcgtcggcc
     9001 cggccgatgg gagccgcttc cgcgaggtgc tcgtcacgcc ggatggatgg ggaggcgaga
     9061 tcggtcccga cgaggacggg gagtgaccga gcgggaacca accaagctcg gcaccgtcct
     9121 gcggaccgcg cgcgaggcca ggttcatcga cctggcccgg gtcgagcgcg acaccaagat
     9181 ccgggtccgc tacctgtcgg ccctcgaaag cggcaactac ggagacctgc ctggctcgct
     9241 ctacacccgg ggcttcctgc gcaactacgg cctgtacctg ggcctggacc cagagtactt
     9301 ggtcgatctc taccggctcg agagcgggag cgccatcgaa cggccctcga tgccatccgc
     9361 gcgtcgtccc atggcggcgc gccagggccg ttcactcgtc gtcagctcgg gtgccgtggt
     9421 ggcggctatc ctgaccctcc tcgtcgtcgc cttcgtggtg tacctggcga ctgagttcgt
     9481 caccttcgcc cgcacccctg acctgcgcat caccgacccc gccaccgatg tcctggcgtg
     9541 gcacggcacc gagtacacga ttcgcggtgt caccgagcca agctccacca tcaccaccga
     9601 tgggttgcgc cagaacccca gcgcgacggc agatgctgac ggcgccttca gcgttctcat
     9661 cggtctcgtc ccgggagcca acgtcatcac cctcgtcgcc aacgacccgc tgaccggccg
     9721 cgacagcgcg gcggtgcggc gcacgatcac ggtggtgacg gccagcccta cgccgagcca
     9781 gctgccatca gcgggagcgg cgggcgggag cacgccgtag gggcgaagat aggggagcgg
     9841 aaccaagcat ggaggcgaga tggccgattc ttcattcgac gtggtgagcg acttcgatca
     9901 gcaggagctg gtgaacgcgc tcgaccaggt gcgccgcgag gttgcgacac gctacgactt
     9961 caagggtgcc aaggtctcgc tcgagctggg caaggacgag atcacgctcc acgcagacga
    10021 cgacttccgc gccggctcgg tcaaggacct gctccaaagc aaggcggtgc ggcgggggtt
    10081 gtcgctcaag gtcttcgact ggggcaagct cgagccggca gctggtagca cggtgaggca
    10141 gcggatcggg ctgcaccgcg ggctcacctc tgaacaggcc aaggagatcg cgaagctggt
    10201 gcgcgagcag ttccccaagg tgaagcccgc catacagggc gatgcggtgc gggtgtcggg
    10261 caagagcaag gacgagctgc agggcgtcat caaccacctg cgcggcctcg actacgcggt
    10321 ggcgctgcaa ttcatcaact accgatgaat ccgcccagcg aggcggacct ggcacgcgcc
    10381 ggcgcgcgtc tcggcacggc cctgcgggcc cgcacctggt cgttggcgac agccgagtcg
    10441 tccaccggcg ggctcatcgg ccaggcgatc accatgaccc ccggcgccag cgactactac
    10501 gccggcggag tgatctgcta ctcgaatcgc gccaaggagg tcgtcctggg cgtcccgcac
    10561 gcgctgatcg cggcgcacgg tgcggtctcc gaggaggtgg ccgccgcact ggcgcagggt
    10621 gccgcgcacc gctttgaaac cgaccttggg gtcagcgtca ccggcatcgc cgggccagat
    10681 ggcggcagcg agcaaaagcc gctcgggacc cactacgtga gcgtggtgcg ccgcggccat
    10741 ccggctcgcg tcgagcacca cctgttcggc cacgaccgaa ccggcaatcg ggccgcggcc
    10801 gcgttggcag tccttgagct cgcgctggac gaggtctcgg cggcagcctg atgcgcgccg
    10861 gcgagcggat ccatctcatc ggcattgccg gctcgggcgc cgcgggggtg gcgctcctcc
    10921 tccaccatgc cggagcccag atcgacggct gcgacgccga taccccttcc ccctacaccc
    10981 cgccgctcga cgcggcaggc atccgcgtca tcagcggcca caacccgtcc cacctggccg
    11041 gtgtcgagcg agtcgtgatc acccccgccc tacgggccaa ccccgacctc cccgagctgg
    11101 ccgcggcgcg cgccgcgggc ctcccgatcg tcacctggca ggcgatgctc ggagagctga
    11161 tggcggccga gggccgcatc ggcctggccg tcaccggcac tcacgggaag tcgaccacca
    11221 ccgcgctgct gggccacctg ctcgagtcgg ccggattgga cccgaccgtc gaggtgggtg
    11281 ccttcatccc ggcctggggc gcgaccgtca ggcggggcgc gggagtgccg ttcctgatcg
    11341 aggcggatga gttcggcgac aacttcctca actaccaccc ggccggcgcg atcgtcacga
    11401 acgtggagat ggaccacccc gactacttcg ccgaccgcga ggcggtcctg gcctcgatgg
    11461 agcgcttcgt ccgtgggatg ggggaggatt cgcggctcgg tggccggctc ctgctcacca
    11521 cggccgcgga cactggctcc caggccctcc tggagcgcct tggagactgg caagggcgca
    11581 ccatgccgta tgggcccggc ggggagatcg aggcgcatga cgtggcgtac gccggcgggg
    11641 gagcgacatt cacattattc gaacacccgt tcgaatcacc cctggcgggg gagcacaacg
    11701 tcctgaacgc aaccgccgcg ctggtcatgg cgcgcgagct aggcgcacca ctcgatgcgt
    11761 tgagcgaggg gcttcgcgag ttccgtgggg ccggacgacg gatggagctg atcgcagata
    11821 ctgcgggcgt gatcgtctac gacgactatg ggcaccatcc aacggaggtg cgtgccacgc
    11881 tggccgccat gcgccagaag gtcggcgatc ggcgcctgtg ggcggtcttc gagccccaca
    11941 tgtactcgcg cacggcgctc ttcctggacg agttcgccca tgcattcacc gatgccgacg
    12001 aagttgtcat cgtcgacatc ttcgcctcac gcgacaccga tgcgtcggtg cgcgcgacct
    12061 cggccgaggc cttggctgac gcgatcgagc gcacctccgc cgtctcgacg atggcgggcg
    12121 gcgacgtgga cgccgcaacc gcgtacgtgg cccaacacct cagcccgggc gacgcggtgc
    12181 tggtgatggg ggcggggaag tcgtaccaga tcgcccaggg gctcaaggcg gcgctcgaag
    12241 gcggcgtcct gacccagtca cgcagccttc ccgaccagtg agcgggtccc gatcggtctt
    12301 cctgggcgcc ctgctggcgc tgggagtggc catcctccag gaggcgctgg tgcggctcgt
    12361 cgaggcgtca ggcgcgcggg accagccggc cgtgatcctc gtcctgttgc tggcggcgtt
    12421 cctcggactg ctgaccgcgg tagctgtccg agctctgcgg ctgccgggcg ccgtcgcgcc
    12481 caccgtgctg gtgatcggct ggatcgtggt gccgccgctc ctgaaggcga tcccagcgcc
    12541 ggtgacgcaa accttcgctt cggatgctgc actcacggcg ccagacgctg ttgccctggc
    12601 ggtggccgtg atcgcggccg cggtgacgct gggggttcag cgggagcgct gagcccccag
    12661 cgatcggaga ggcgtaaacc ttgccccggc acctcgcgtg aacgaagatg ccacggtgcc
    12721 agatccagag tcacccgtga gcggccccga cccgaccggc tcaccagcgg ctcagcccga
    12781 ctcggtcacg cgcgggttcc tgttctccga tctcaggggc tacacgcagt tcgtagagac
    12841 ccgcggcgcg acgaccgcgg cagcgctcct ggtccgctat cgagagctgg cgcgcagcgc
    12901 catcgcccga ttcggcggag ccgagatcaa gaccgagggc gatagcttct acgttgtctt
    12961 caggtcggtg agctcggccg tccgctgcgg cctcgccatt acggccgacg cagcggccgc
    13021 ctcgatcgag catccgtacg aaccgataca ggtgggcgtc ggcatccacg ccggcgagac
    13081 cgtggagtcg gacggtgggt atgtcgggtc ggcggtcaac atcgccgcgc gaatctgcgc
    13141 ccaggctgcg tccggggagg tcctcgtcag tgagacggtg cgcgccctga ccaggagcct
    13201 actgaccgtg cggttcgagg aacgcggcag gcgccagctc aaggggatcg ccgacccgat
    13261 cgcgctgttc gcggtgctcg atgccggtcc cggctccatc cccgcggcgc gaggccggcc
    13321 acgctggctc cgcggacgcc ggttgcttgt cgcaacgctc ggcggcgcgg ccctgctggc
    13381 gggcgccgca gcctttggct ggggggctct ccatccggcc gggctgccgc cgggtccatg
    13441 gaggatcgga ctggacatgc cactgacagg ggatacggcc tcccgcggcc aaccgatgca
    13501 gaatgcagcg cagctggcca ttcgtgatgc gaacaaggcc ggtggaatcc tgggctcgat
    13561 gctcactctg aaaagctacg acgatgccgg cgcgccgccg aacggccagg accccgacca
    13621 gggtgctgcc aacgcgacgt cgatgattgg cgacccgagg acgattgcga tgatcggtcc
    13681 gagcggttcc agcgttgcgc gcgccatcct gccgctgacg aaccaggctg gcctcttcga
    13741 gtgcagccca gccaacacgt cgccggggct gacgaagcca cgcgacggcg ccctcgacct
    13801 gcgctcggcg caccctgacg agatcaacta cgtgcgcgtt gcaccctctg atgacatcca
    13861 ggccctggct ctggcgtcgt tcgcgtcccg agacctcgcg gcggacaagg tagcgctcgt
    13921 gatcgatgac acaggagatg gccgggagat cgccgatggc ttccagcagg aatacaccaa
    13981 gctcggtggg gtcgtcatcc gtcgtgcgct gaacgcgggc gcggatccga tgtccgtgct
    14041 tggcccgtcg gcggatggca ccccagcacc tcaggtcgtc ttctttggcg gctacaccca
    14101 gaccggcgcc gcccagctgc gcggggccat gagcaaggcg ggcctcggcg cggttccgtt
    14161 cctcagctgg gacggcatcc tggacgggag cgccgccgat acgggctcgt tcattcagct
    14221 gacagggtcg gccgccatcg gctcatacct cgcccatgcc tccctcgggc cgcacaaggc
    14281 gaactttgcc gatgcgtacc gcgccgccta cggagaggag ccggacgagt actcggccgc
    14341 cgcgtacgcc tgcggagagg tcatcgttgc gtcgttgcgt gcgatcgcag ccaacagccc
    14401 cagcaccgac ggcctgcgca gggcgcttcg tacctacgcc gtcgacccga gccatcaata
    14461 cgcaaccgtg ctcggcaccg tcggcttcga cagtaacggc gactcgaccc agcagttcgt
    14521 gaccttctac cgcgtcgatg ccggtgactg ggtgatcgat aagcagcagg acttcggtcc
    14581 cgcccggtag aacggcggct cctgtgatgg cagtaccgaa aggtcaacgt cacataacat
    14641 ctcgatgagc gtgaatctga gactgtttgt ggcaccagaa ggggcggctg gcaggccgaa
    14701 ggtgggggag tgtagagtcg ggtttgcatt tggctcctta cacgagctag ctgccacggc
    14761 cgggagtcat gtgctcgccg gccatttcgt cggccgccac cataccaccg gcgcgcaagg
    14821 gcgcgggcgt aaactctcgg cggagggttt cggccaatga tggggtgttt gctgctcgcg
    14881 gtgcttctga tcttcgcggt cctcgtgccg ccgctcggga tcccgctgct gctgctggtg
    14941 ctggttgtga tgatcgtcgt tttcatcgtc cgcggctcga gccaggctgc cggatcgctt
    15001 ctgagccgtc ccccagacc
//
LOCUS       DATCQR010000063         4526 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_18339, whole genome shotgun sequence.
ACCESSION   DATCQR010000063 DATCQR010000000
VERSION     DATCQR010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4526
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18339"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1074)
                     /locus_tag="VIN32_02090"
     CDS             complement(<1..1074)
                     /locus_tag="VIN32_02090"
                     /inference="COORDINATES: protein motif:HMM:TIGR01304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GuaB3 family IMP dehydrogenase-related protein"
                     /protein_id="HEY8921397.1"
                     /translation="MIDPAILSPDATSTRLRRAYGFDEVALVPGAVTVDPAEIDLSTE
                     IGGHRLEIPFIASAMDAVADADFAARMSRHGGLAFINLEGLYTRYEDAAPTIERVASA
                     ASNQEAAELLAAAYAAPVRDDLITALITRVKAQGGIAAVATTPGSAWHHAEVAAEAGA
                     ELFLVQSQVSSAQHISTGGRVLSLSDLTGALKIPVLVGNTVSGEAAYQLMHQGAAAIL
                     VGVGPGAACTTREVLGIGVPQVSATLEVAAARDRYQQETGRYVPVITDGGMKTGGDIA
                     KAIASGADAVMLGSPLAGALEAPGRGYNWGMAAPSPTLPRGTRIKIGERLPLEQILFG
                     PAHTTHGTQNLVGALRQSMASLGA"
     gene            complement(1119..1679)
                     /locus_tag="VIN32_02095"
     CDS             complement(1119..1679)
                     /locus_tag="VIN32_02095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921398.1"
                     /translation="MTAALGRLAGGVACVAMLAACASGLEHPTPHGSSFESADFRFVA
                     PTSWNVRPSTATTSAEDGIVLYVANQALRDDCPASGSVVACQAPIDGGLRRGGILITW
                     VRHSCVARGCDLPAEELISIGNRQGVKVPATQACEGMRTTDASVYYVTVTPQRVDGLF
                     ACARDPSDATRSALLGFLDAIQWRIP"
     gene            complement(1688..3010)
                     /locus_tag="VIN32_02100"
     CDS             complement(1688..3010)
                     /locus_tag="VIN32_02100"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019120865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEY8921399.1"
                     /translation="MSVIAVVGAQWGDEGKGRVVDYLATDADLVIRYGGGNNAGHTVV
                     NPHGHFKLHLIPSGIFYPATRNLLGGGVVINPPALVRELDGLAAAGFDCANFFISERA
                     HLVMPYHVLLDQLEERERGTAKLGTTSRGIGPAYVDKVARRGIRVVELMEPDDFRAHL
                     RVALARIRRIVTGYFGAGGDDDELRPAITEATDEDRIATEYLAAAERLRPHVVDGQSL
                     VDAALVEGERILLEGQLGTMRDIDWGTYPYVTSSSPIPGGASIGAGLPAVAIDKVIGV
                     AKAYTTAVGAGPLPTEMLDAEGEALRERGVEYGTSTGRPRRCGWYDAVAVRFSVRLAG
                     YSSIALTKLDVLDESQRIRVCVAYRDPQTGSEWATVPASTSVYERLEPVYQELPGWQA
                     DTTGCREFNQLPAAARAYVERLEELAGVPIGLVSVGPERDQMIVRGAG"
     gene            complement(3136..3444)
                     /locus_tag="VIN32_02105"
     CDS             complement(3136..3444)
                     /locus_tag="VIN32_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013432187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain containing protein"
                     /protein_id="HEY8921400.1"
                     /translation="MYADKTISCRDCGMEFVFSAGEQQFYAEKGLVNEPQRCPSCRAV
                     AKQNRALGISSGGGTGQREMHAAVCAECGGQALVPFLPRNDRPVYCSSCFDKVRATRT
                     "
     gene            3745..4194
                     /locus_tag="VIN32_02110"
     CDS             3745..4194
                     /locus_tag="VIN32_02110"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HEY8921401.1"
                     /translation="MASTACKGLTTLVMVLLLIACSPSADRRNVPPGTDRVVELSMEG
                     MHFIPDRNEVKAGELVAFVVTNPNDVAHEVFIGSEADQMAHHAVHMAVASPGQAQIPH
                     GGYGIFLAPHETGVLSYRFDTPGEIMLGCHLPGHWEAGMKAVVIVEQ"
     gene            complement(4201..>4526)
                     /locus_tag="VIN32_02115"
     CDS             complement(4201..>4526)
                     /locus_tag="VIN32_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HEY8921402.1"
                     /translation="RGLKQLSRELKVPVIALSQLSRGVEQRGSAEPRLSDLRESGSIE
                     QDADVVIFLYRDGEANADSDVELIKAKVAKHRNGPIGEVALQFRKTNTRFYTMAARET
                     EAVTY"
BASE COUNT          712 a         1573 c         1539 g          702 t
ORIGIN      
        1 cgcccccagc gaggccatcg actggcggag cgcacccacc aggttctgcg tcccatgcgt
       61 ggtgtgggca gggccgaaga ggatctgctc cagcggcagc cgctccccga tcttgattcg
      121 cgtcccgcgc ggcagggtcg gagacggcgc cgccatgccc cagttgtagc cgcggcccgg
      181 cgcctccagg gcccccgcca agggcgagcc gagcatgact gcatcggctc cgctggcgat
      241 cgccttcgcg atgtcgccac cggtcttcat gccgccatcg gtgatcaccg ggacgtatcg
      301 gccagtctcc tgctggtacc gatcgcgggc cgccgccact tcgagcgtgg cgctcacctg
      361 tggcacgccg atgcccagca cctcgcgcgt cgtgcacgcg gcgccaggtc cgacgcccac
      421 caggatcgct gcagcaccct ggtgcatcag ctgataggcg gcctcaccgc tcacggtgtt
      481 gccgaccagg accggaatct tgagcgcccc ggtcaggtcg gagagcgaga gcacccgccc
      541 gccggtcgag atgtgctggg cactggagac ctgcgactgg acgaggaaca gctccgcgcc
      601 ggcctcggcc gcgacctcgg cgtggtgcca tgccgagccg ggcgtcgtgg cgaccgccgc
      661 gatcccgccc tgggccttga cccgcgtgat gagtgccgtg atcagatcgt cgcgcaccgg
      721 cgctgcgtag gcggcagcca gcagctcggc ggcctcctgg ttcgaggcag ccgatgccac
      781 gcgctcgatg gtcggtgcgg catcctcgta gcgggtatag aggccctcga ggttgatgaa
      841 ggcgaggccg ccgtggcggc tcatgcgcgc ggcgaaatcg gcgtccgcga cggcgtccat
      901 ggcagacgca atgaatggga tctcgagccg atgtcccccg atctcggtgc tgaggtcgat
      961 ctcggcgggg tcgaccgtga cggcacccgg caccagcgcc acctcgtcga atccgtacgc
     1021 ccgccgcagc cgagtcgagg tcgcgtctgg ggagagaatc gccgggtcga tcaacaacgc
     1081 ctccgctcag gtgtcggggg agtatagggc gctggggatc aggggatccg ccactggatg
     1141 gcatcgagga agccgaggag ggcggagcgg gtcgcgtcgc tcgggtcacg cgcgcaggcg
     1201 aagagaccgt caacgcgctg gggagtgacc gtgacgtagt agaccgatgc atcggtggtt
     1261 cgcatgccct cacatgcctg cgtcgccggc accttgacgc cctgtcgatt gccgatggag
     1321 atgagctcct ccgcaggcag gtcgcagcct cgtgcgacgc atgagtggcg aacccaggtg
     1381 atgaggatcc cgccgcgacg gagcccacca tcaatcggcg cttgacaagc gaccaccgat
     1441 cctgatgcgg gacaatcgtc gcgcagcgcc tgattggcca cgtacagcac gatgccgtcc
     1501 tcagccgagg tcgtggctgt cgagggacgg acgttccagc tggtcggcgc gacgaaccgg
     1561 aagtcagcac tctcgaacga cgagccgtgc ggtgtggggt gctcgagtcc cgatgcgcag
     1621 gccgcgagca tggcgacgca ggccacgccc ccagccagcc gaccgagggc cgcggtcatt
     1681 cgcgccgcta cccggctccg cgcacgatca tctggtcgcg ctccggacca accgagacga
     1741 ggccgatcgg cacgccggcc aactcctcca gccgctccac gtatgcccgg gccgccgcgg
     1801 gaagctggtt gaactctcgg caaccggtcg tatcggcctg ccagcccgga agctcctggt
     1861 agacaggctc caaccgctca tagaccgatg tcgaggcggg gaccgtcgcc cattcggacc
     1921 ctgtctgcgg atcgcgatag gcgacgcaga ccctgatccg ctgcgactcg tcgagcacgt
     1981 cgagcttggt cagggcgatg ctcgagtagc cggccagccg cacgctgaat cgcacggcca
     2041 ccgcgtcata ccagccgcag cgccgtggcc gcccggtcga tgtcccgtac tcgacgccgc
     2101 gctcgcgcag ggcctcgccc tctgcgtcga gcatctcggt cggcagcgga ccggcgccga
     2161 cggcggtggt atacgccttg gccacgccga tcaccttgtc gatcgccacc gccgggaggc
     2221 ccgccccgat cgatgccccg ccggggatcg gcgaggagga ggtgacatat ggataggtcc
     2281 cccagtcgat gtcgcgcatg gtgccgagct ggccctcgag caggatccgc tccccctcga
     2341 ccagcgccgc gtcgacgagg gactgcccgt ccacgacatg tggccgcagc cgttccgcgg
     2401 cggccaggta ctcggtggcg atgcggtctt cgtcggtcgc ctcggtgatg gcggggcgca
     2461 gctcatcgtc atcgccaccc gcaccgaagt agccagtgac aatgcgtcgt atccgagcca
     2521 gggcgacacg caggtgcgcc cggaagtcat cgggctccat cagctcgacc acccggatgc
     2581 cgcgccgggc caccttgtcg acgtaggccg gaccgatgcc acggctggtg gtccccagct
     2641 tcgccgtccc gcgctcccgc tcctcgagct ggtcgagcag cacgtggtag ggcatcacca
     2701 ggtgagctcg ctcgctgatg aagaagttgg cgcagtcgaa gccggccgcg gccaggccgt
     2761 cgagctcccg caccagcgcg ggcggattga tcaccacgcc cccgcccagc aggttgcggg
     2821 tcgcgggata gaagatgccc gacggaatca ggtgaagctt gaaatggccg tgcgggttca
     2881 ccaccgtgtg cccggcgttg ttgccgccgc cgtagcggat caccaggtcg gcatccgtgg
     2941 cgaggtagtc gaccacgcga cccttcccct cgtcgcccca ctgggcgccg acgacagcga
     3001 tcacggacat gggcaggcac agacctcagc gaatggagga cgcatcgtag cgccggcggg
     3061 cacaccgcgg atgcgggaac gccgccctgg gaggcggcgt tcggatcggc gtgctccgat
     3121 gaaatggcgg acgggtcagg tccgggtggc acgcaccttg tcgaagcagc tgctgcagta
     3181 gacagggcga tcgttgcgcg gcaggaatgg cacgagcgcc tgcccaccgc actcggcaca
     3241 cacggcggcg tgcatctcac gctggccggt gccgccaccc gagctgatgc ccagggcccg
     3301 gttctgcttg gccacggcgc ggcagctggg acaccgctgc ggctcgttga ccagcccctt
     3361 ctcggcgtag aactgctgct cgccggcgga gaacacgaac tccatcccgc agtcgcggca
     3421 gctgatcgtc ttgtcggcgt acacctctac ccctgccctc catcgtcgcg cccgggccga
     3481 tctggacggt gctgttcctc gggcctgggc tgatcggcgg cgtcgcgctg gatcggtcgg
     3541 actcgggtag aacattggac cggacgttcc gcggccgact atacacacgc cttcaaggcc
     3601 tgcctagagt gcaatcggcg cgcgggcttc ccccggcaag tgccattggg accgatcccg
     3661 cccgctcaat tcgggccatt cgcaccgtga cacccaccgc tggggctggt aggccttctg
     3721 cataccgttg cagaaggtgt accgatggcc tcaaccgcat gcaaggggct tacgacgctc
     3781 gtgatggtgc tgctgctgat cgcctgctcg ccgtcagccg accggcgcaa cgttccacct
     3841 ggcaccgatc gcgtggtgga gctgtccatg gaggggatgc acttcatccc cgaccgaaac
     3901 gaagtcaagg cgggcgagct ggtggcgttc gtcgtcacca atccgaacga cgtggcgcac
     3961 gaggtcttca tcggcagcga ggccgatcag atggctcacc acgccgtcca catggccgtt
     4021 gcctcacccg gccaagcaca gatcccgcac gggggctacg ggatctttct ggccccgcac
     4081 gagaccgggg tgctgagcta ccgcttcgac acgcccggcg agatcatgct cggctgccac
     4141 ctgcccggcc actgggaggc gggcatgaag gccgtggtga tcgtcgaaca gtagctttgg
     4201 ctagtaggtg accgcctccg tctcgcgggc ggccatggtg tagaagcggg tgttggtctt
     4261 gcggaactgc agcgcgactt cgccgatcgg cccgttgcgg tgcttggcca ccttggcctt
     4321 gatcagctcc acgtcgctgt cggcgttcgc ctcgccgtcg cgatacagga agatcaccac
     4381 gtccgcatct tgctcgatgg aacccgactc cctcaggtcg gagaggcgcg gctccgcgct
     4441 ccctcgctgc tcgacgccac gtgacagctg ggagagggcg atcaccggca ccttcagctc
     4501 acgggagagc tgcttcaggc cgcggc
//
LOCUS       DATCQR010000064        21565 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_49366, whole genome shotgun sequence.
ACCESSION   DATCQR010000064 DATCQR010000000
VERSION     DATCQR010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 21565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21565
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49366"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            114..1340
                     /locus_tag="VIN32_02120"
     CDS             114..1340
                     /locus_tag="VIN32_02120"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018131981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEY8921403.1"
                     /translation="MRQHDPARSPVVVDGLRTPFGRYGGALREVRPDDMAAHVIRALL
                     ERTGVDPGAVEDVILGAANQAGEDNRNVGRMAALLAGMPIEVAGQTVNRLCGSGMQAV
                     IDAAHAITAGDGDLFVAGGVESMTRAPFVMAKASAAFPRGEQTLYDTTLGWRFTNPRL
                     ADAYYPYSMGETAENVVERCGVSREDQDRFALQSHQRWAAAQAAGRFTDELVPIEAPA
                     SKGATTTVDRDEHPRPDTTLEQLAALKPAFLRDGSGSVTAGNSSGINDGASALLVTSE
                     ARAAELGLKPMARLLSSAVAGVDPATMGLGPIPASRKALDRAGLSVADLDLVELNEAF
                     AAQALPVIRELELDPEKVNVNGGAIAIGHPLGASGARLVATLLHEMRRRGARHGLATM
                     CIGVGQGIASVWESVE"
     gene            1337..1519
                     /locus_tag="VIN32_02125"
     CDS             1337..1519
                     /locus_tag="VIN32_02125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921404.1"
                     /translation="MNASERTGTVDPIGRPAGWLSLGFVAGVVAPLAVAAAAYVLWWV
                     SDRLLYIGPFDRAAFG"
     gene            complement(1539..2528)
                     /locus_tag="VIN32_02130"
     CDS             complement(1539..2528)
                     /locus_tag="VIN32_02130"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03557 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEY8921405.1"
                     /translation="MTKIGYAAMLEQFHPNDLLEWCPQAEAAGFSAGFMVSEHFHPWT
                     PQQGQSAFAWSFMGALGQRTSLPFGVAVTCPGFRYHPAVIAHAAATLGAMYPGRFWLG
                     LGAGEALNEHVIGGEWPEVGIRSAMLFEAIEVITKLFSGNVVKHDGEYFTLESAKLYT
                     RPDQPVPIYVATAGPMNAKRTGRHADGMITVGAADEKIHALWSKFAEGATDGGKDPSG
                     MPKLLQIHLSWAETQQVAEANAVREWPNGAMAFPKQDIRNPEDVANMAKLIRPEHFTN
                     RVLISADLDEHAAHIQKFVEMGFDEVHLHNVGRNQAEFISAFGEKVLPQLRLG"
     gene            complement(2618..3346)
                     /locus_tag="VIN32_02135"
     CDS             complement(2618..3346)
                     /locus_tag="VIN32_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921406.1"
                     /translation="MKNIKTLGMAGGLVAAALVGGTLINAALAAPSSPNPNASAANPA
                     DKAAYCDTWQKTFADQLGVSVSDLLPAGKAASIAAIDAAVAAGDMTAERATALKDKIA
                     AADGSNACRFFGHPFAGGGNGGKAHFGGPLLSTAADALGMDPGALVQALRNGDSLKDV
                     ATAQGKDYAAVSKAIHDAAKTKLDAAVAGGLDQARADAMLAQIDQALASGDLSQLHPG
                     HGHGGGFPGLGADKPDGSEEPSSS"
     gene            3481..4194
                     /locus_tag="VIN32_02140"
     CDS             3481..4194
                     /locus_tag="VIN32_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007498313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY8921407.1"
                     /translation="MVHARILIVEDEPQLRGLLRLYLERAGYAVTDAGDGAAALAAFA
                     ADGADLVVLDLMLPGMQGEAVLEALREAADVPILITSAKRSDAERIAGLRLGADDYLA
                     KPFNPHELTARVAAILRRSRPAAAEGTEAIISLGAGRLVLDPSSRRYTLSGGEGGRLT
                     PGESALLVALARRPGAVLAREQLLEAVARRPEEVYERVVDVHVANLRRKLGDDATDPW
                     LIETIPSTGYRLVAARDPA"
     gene            4191..5525
                     /locus_tag="VIN32_02145"
     CDS             4191..5525
                     /locus_tag="VIN32_02145"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY8921408.1"
                     /translation="MTVGQSEIRGQPLGLRLALLLSAAVLAVLLIAGLAVNRVVSGSL
                     ERELSAAERDRITVVAGAIAGVDLNAPKAMHGVEVVLRRVANTMRGHVQLIGPAGDLL
                     VDVGRLPPGVSTETISEPITGSGATLRIDVPRADRAFLGVFNLSLLVAGLLATLAVGL
                     IALVLSSRLTRPLQAVAAAARRLGHGDLDARATGGPDRESNELADAFNAMAGRVQRSE
                     MLRRRAASDMAHDLATPATVLESQLQAMVDGVVPADREQLDRARAAAGALSGVILQLG
                     ELVDAESAVMQRRPRVISLGELLAEVERAIVPLFRERGVDIEVAEVPASLRVDVDSTQ
                     VGRALRNVLANAAQHSPREATVHVAVEVAAGEALVRVNDAGPGIAPEDLAHIFERFYR
                     ADQARGAGEPRSGSGIGLTIARELLAANGGRIAVERTGLDGTTFAIGLPLAG"
     gene            complement(5522..6127)
                     /locus_tag="VIN32_02150"
     CDS             complement(5522..6127)
                     /locus_tag="VIN32_02150"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEY8921409.1"
                     /translation="MSDAEAPAPRPMIRGEKVWLRPSEPSDIIHDSTYAGDAEVGHFL
                     GAKTPMSKAGAERFSAEIFSQSPMDPNGYGYAICLLGSEESIGTVFLRNVDKVNGSGI
                     VGIFIGDRRYLGQGYGTDALNALVDLGFGELRLERIELEVFDFNERAVRSYEKAGFQT
                     DAIQRRARFHRGAHHDVHVMSILREDWLAMPRKRSWELAGP"
     gene            6198..7118
                     /locus_tag="VIN32_02155"
     CDS             6198..7118
                     /locus_tag="VIN32_02155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921410.1"
                     /translation="MEPSSEASLEAWYSRFPEGWWGYHWSLPRPMSAVEIVGSGTLDA
                     RLMATLWAVTSRRRSVMLASEAPMAGKTTTLSALVDFLPPGTVGVFLRGWRGDFDWTD
                     EIGPERGYLLVNEMSDHLPIYVWGRNARGALELAGRGYGMGATMHADSLDEAVSVLRD
                     QLGATDADLGGLTIYLQYSAYRTPAGMYRRVEEAWHLRLDEGGTLAPVRLAALDGGRS
                     PSLTGAQRDPEPPLLASDGGRSRRRLVHDREAYAVLAASLRLVPDELEAELAARSEYI
                     AALADRGVCDPASVAAAIPLYPSLPEGATA"
     gene            7115..8530
                     /locus_tag="VIN32_02160"
     CDS             7115..8530
                     /locus_tag="VIN32_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007923295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase"
                     /protein_id="HEY8921411.1"
                     /translation="MTDRPTIGTLGELRAGGYRSRSVKEEMRANLVDRLRRGESMLPG
                     IIGYDETVLPQVENAILAGQDIILLGERGQAKSRIARSLIGLLDEWMPVVAGAELHDD
                     PLRPVSPFARGLVAEQGDATPIAWVPAADRYGEKLATPDITIADLVGEVDPVKVAEGR
                     YLSDELTIHFGLLPRTNRGIFALNELPDLAERIQVGLLNLMEERDVQIRGYKVRLNLD
                     LFVVASANPEDYTSRGRIITPLKDRFGAQIRTHYPEDPETEIAIMEAEWLAFDDETAT
                     SVPVQVPSFMKEIVAELSQLARRSSEVSQRSGVSVRVTIANHETLVAAALKRAVRLGE
                     PIGAPRVSDLGAIVASTAGKIELETLGDETREEKVIEKLVQRAVVNVFNRHFSLSELV
                     SLVERFEAGAEVEVGDAVASDQLQRTLAELPELSRAVGRLDIGESPAGVASGVEFVLE
                     GLHLNRRLNKERRGGSVRYAR"
     gene            8534..10567
                     /locus_tag="VIN32_02165"
     CDS             8534..10567
                     /locus_tag="VIN32_02165"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY8921412.1"
                     /translation="MSSFRYSHWDGSQSLPPFDADDVLEALSDDILAEGDIRRALQRL
                     MQRGMRGTRGGDVPGLRRIVEQLRARRAEELSRANLDGMLDDLAGRLEEIVEQERAGI
                     ERRVHEAEQRALDAPPGPAQDQARMAEQVLRRTGQQRRDRLDALPDDLAGRLAGLRDY
                     EFMDADARDAFNALTDEIRQQLLQTYFQGLKEGVAGISAEDLDGVRAMVRDLNKLLEK
                     HAAGSDTSADFASFMVRHGHYFPPGIETVEQLIDHLHQQASKMASLMASLSPEMRAEL
                     QQMMDELLRDDRLRWDMARLAGTLQALRPDTPFGEPYPFSGDEPLGLPEALAAIDRQQ
                     QFDAMEEELLAARDPDSLEQIDEEALQALGGDEAAGDLEQLRALTRALEEAGYLERGD
                     DRLELTPRAARKLGMRALTDIFSRLRRESLGGHALPTSGIGGEQTDETKPFEHGDPFS
                     VDLNRTLFNAMARNGPGTPVRIAPADFEVHRSEETTVSSTVLLLDMSRSMLLRGCSTA
                     AKRVAMALHTLIHTKYPRDRLYVVGFAYYARQIKPEAIATLSPYEFEYGTNLQHALII
                     ARQLLGRRSGGNKEIVVITDGEPTAHIANGQVEFAYPPTMRTMQSTLREVGRATREGI
                     VINTFMLERSRYLSEFVDLMSRINRGRAFYVEPEHLGEYVLVDYVSKKRKRVA"
     gene            10567..11478
                     /locus_tag="VIN32_02170"
     CDS             10567..11478
                     /locus_tag="VIN32_02170"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY8921413.1"
                     /translation="MKVGVQLPEVEWVARWPQQREMARTAEEIGLDSIWVGDHLLYRG
                     DGLPSRGPWAAWTSLAAIAAVTERVAIGPLVTAASFHNPAMLAKQAATVDEISGGRLI
                     LGLGAGWNEAEYLAYGFPFDHRVSRFEEAFTIIRTLLTEGRCDFHGTYYDLQDCELLP
                     RGPRHAQGEGPPLLVGSMGERMLGITLPWVQAWNAWFTWFGNTVEGYRPLRDTVDAAC
                     RAAGRDPAEVERTLALLVAFPGAKGRTLGDLKEPEVEPIPSNPATLAATLRDFEAEGV
                     AHVQLVLDPINAETIAGLAPMLKELRA"
     gene            11503..11880
                     /locus_tag="VIN32_02175"
     CDS             11503..11880
                     /locus_tag="VIN32_02175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921414.1"
                     /translation="MEGPPARLPLGPNGRLEAEPLTNAVGNHAVLVREGEQPRALVTV
                     EAGSPERQRVIIVPLDNDVEMRVDGDALAIWLSQGSIGPAPSDVPGWANAIGFGLVIA
                     AVGFTVLGSVTFFGWLFRTLGWL"
     gene            11871..12164
                     /locus_tag="VIN32_02180"
     CDS             11871..12164
                     /locus_tag="VIN32_02180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921415.1"
                     /translation="MALTPPTSGLIAMRIGQEFGPPEEFERSLERAIERGGERGATIV
                     AVLDLGDLATHIPQVDGPSWNTVPLVHLHRGQQPTEEDWAVANAIVERLERYR"
     gene            12169..12675
                     /locus_tag="VIN32_02185"
     CDS             12169..12675
                     /locus_tag="VIN32_02185"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEY8921416.1"
                     /translation="MDEDLLLGLRAMMGHFATGVTVVAARHGPLLAGMTANAIASISL
                     DPPLLMASINRGSETHVAIVGSHSFAVSVLGAGQRAIADCFALPTTAGKLQRFCDAPW
                     HEAETGSPILDGAIAFFDCRLHESHAAGTHTIFIGEIVAAGYEEGGEPLLWFGSRYRR
                     IAAEPGEG"
     gene            complement(12672..13979)
                     /locus_tag="VIN32_02190"
     CDS             complement(12672..13979)
                     /locus_tag="VIN32_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005169073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY8921417.1"
                     /translation="MTDEATPWQELRAGVPPVWARYTDLVVERGIGSWIETIEGERYL
                     DYTCGIGVTNTGHAHPRVAAAIAEQAGRIIHAQQNILHHKPGLELHARLPRTFPGLRG
                     ADEAGLFLSNSGAEAIEASVKLAKYATRRPAIIAFRGGFHGRTHGAMALTSSGVKYRG
                     HFEPLMGGVHFAPYPYALRNPTGRNAEAAVAFSLAGIEELFATVIYPDDVAAFLVEPI
                     LGEGGYVVPPDSFLPALREIADRHGILLIADEVQTGMGRTGRMWATDWYDARPDILVI
                     AKGIASGMPLSGIMARRELLDRLGPGAHGGTYGGNAVACAAAVATLDVIESEGLLANA
                     ARQGERLLAGLHGAAVGHSCVAEVRGRGLMIGIELAQGPELTPRPDLAKAILHEAFDR
                     KLLLLSCGTYGQVVRVIPPLVTTDEEVETAIGVISESLAAVGA"
     gene            complement(13976..14803)
                     /locus_tag="VIN32_02195"
     CDS             complement(13976..14803)
                     /locus_tag="VIN32_02195"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8921418.1"
                     /translation="MHRLTRWLLPGFTALVVAFLFAPIVVMIAFSFNSPRGHQNITWQ
                     GFTLDNYLTIWNRSDITDPMVTSLTIAVLSTIVATVFGTMIALSLTRYQFHGRGILNL
                     LIFIPMTAPEVILGASLLTLWVAIGLGRGLPTILVAHIMFNISYVVVTVRARLIGFNR
                     SLEEAGMDLYATESTTFWKVTFPLIFPGILAAALLAFALSIDDYVITLFSAGHTVTFP
                     LWVYGASRIGIPPEVNALGTLFFLIAFVFIIVQVWAQRRGGRDAQQTGVPIAPVASR"
     gene            complement(14803..15660)
                     /locus_tag="VIN32_02200"
     CDS             complement(14803..15660)
                     /locus_tag="VIN32_02200"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8921419.1"
                     /translation="MNRAGRRLVPYLLLLPGTAWLLLFFAVPMAFMLVMSLQEGSLST
                     GYHLTWNWGIYPEVASRYGVLFVRSVSYALATTVLTLAIGFPMAYTIAFYGGRLKNAL
                     VLVVIMPFFVSFVIRTLNWRMILSDNGLVFGTLKDAGLLEQSFRFLATPAAVIFGLTY
                     NFLPFMVLPLYVALEKIDRRLIEAGTDLYASRAQAFWRITFPLALPGVVAGSLLTFIP
                     AVGDFINADIIGARSPEVAMVGNVIQRLFLSVNDFPAAAALGFVLVAATMLLVGVYTR
                     LVGTDRLTS"
     gene            complement(15657..16907)
                     /locus_tag="VIN32_02205"
     CDS             complement(15657..16907)
                     /locus_tag="VIN32_02205"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HEY8921420.1"
                     /translation="MRDPEERGEIEVAIDRILRQDPMSRRTFMRRTGRGGLALGAMLT
                     LPSLLAACAPAKKKLEWANWPLYIDIDDKAAPAPTPYPSLNKFIKDTGIDVHYVEAIN
                     DNEEFYGTIQPDLAAGNATDWDIVSPTSWMVKRMADQGYLEEIDHSKLPNWTANCADY
                     AKGLWFDADNKYSVWWQGGITGIGYDPKLTGREITKFDDLLDPEFKGRVGGFSDMRDM
                     MTLCLASLGIDPSTATVAQAQQARDKLLTAAKAGQFRGFYGNEYYDALAAGDLVASVA
                     WSGDITQMQLNDNDKVLFVVPSDGAPRWNDNLVIPKKAAWIDQAHKLINYWYDPAAAT
                     TLSEYVGYFTPVKGVSELIKADADAYRADGDTENADYYDAVGPTVTPTPDQLAGTFDY
                     PQFENPDDEKAWNDMFLEVTTAAG"
     gene            complement(16900..18057)
                     /locus_tag="VIN32_02210"
     CDS             complement(16900..18057)
                     /locus_tag="VIN32_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018224209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8921421.1"
                     /translation="MPQSVDVRLDHITKRFHEVVAVDDLSLEVERGEFFSMLGPSGCG
                     KTTTLRMIGGFEEATSGTIYLGDADVTGLPPFKRDVNTVFQNYALFPHLSVFENVAFG
                     LRRRKVAASEIANQVGAMLELVELPGYEARKPSQLSGGQQQRVALARALINRPRVLLL
                     DEPLGALDLKLRKQMQLELKRIQTEVGITFIYVTHDQEEAMTMSDRIAVMRAGRIEQL
                     GNPEELYERPRTAFVAGFLGVSNLLEGEIAGGDGSLVTVKLHDGTVLRAPGEGVATTG
                     PVRIGVRPEKLRVDARVEAGASNGLNALSGTVLDASYIGVSTQYLVQTAEGHRLTVYA
                     QNLDTAGAGELLAGGQHVQLTWKPQHTFVISGPAEHVAGDPHLTEEGESHA"
     gene            18182..19333
                     /locus_tag="VIN32_02215"
     CDS             18182..19333
                     /locus_tag="VIN32_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011024968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY8921422.1"
                     /translation="MDFALSAPHVEIRRTVRDFAEREIEPVADEMERSGEFPAEIIRK
                     AAALGLLGVPYPEEVGGTGLDSLAYAITVEELSRVSGSVGIIVSAHTSLGCAPLFTAG
                     TPEQKERYLRPLASGAKLGAYGLTEAGAGSDSRGTRTRAHRDGEDWVLTGSKRFITNA
                     GVADTYIVTAVTDRNADSGKISAFIVEAGTPGFSIGRMEEKMGLHASNTGELLFDDCR
                     IPSENLLGEEGDGDRLFLKTLDGGRIGIGAMALGLAQAAYEAASAYSKDRQQFGRPIA
                     SFQGIAFKIADMATQIDAARLMVYRAAWLKDSGAPYSTEAAMAKLFASEVARQVTNDA
                     VQVHGGYGYITEYRVERYLRDAKLTEIGEGTSEIQRMVIARNLLGVRAM"
     gene            19330..21297
                     /locus_tag="VIN32_02220"
     CDS             19330..21297
                     /locus_tag="VIN32_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY8921423.1"
                     /translation="MSGPLFGTDTDAAAWRPSPAYLQRSRLARFLAATGSADLEALQA
                     RAVADPAWFWQAAVADIGVRFDPEPGTVLDTTRGIEWARWFSGAGFNYVRMALDEPAA
                     ARPDEMALVWEGEDGEVRQFTRAQLRWAVDRAAHAMVALGVERGDRVGIFLPMIPEAV
                     IAVLAVGKLGAIYTPIFSGYGADAVASRLADCEAKLLVTADGFARRGKPVPMKATADE
                     ALARASSVERCLVVRHAGGEVAWRPERDVWWDAAVTSASDQPLESAATDANDPFMIIY
                     TSGTTGRPKGALHVHGGFPIKAAVDLAHSFDLQAGDTLFWFTDMGWMMGPWAVAGAPM
                     LGATLFIYEGTPDYPGPDRLWGLVERHRITHLGISPTLVRALMTHGDEPPGGHDLSTL
                     VVLGSTGEPWNPDPWWWLFKTIGGSRLPIINYSGGTEISGGIVGCTTIAPIAPCSFSG
                     PCPGMAADVVDAEGRPVRDEVGELAIRQPWPGMTQGFWHDRERYLEAYWSRLPGVWVH
                     GDWARVDPAGFWYISGRSDDTIKVAGKRIGPAEVESAAVSHPLVAEALAIGVPNEIKG
                     ETIALFAVVRGDADRGDGLAADIGQTVVERLGKSFRPGAVHLVPSLPKTRNGKILRRL
                     ARAAYLGLDPGDMSSLEDASTLEPIRALGGP"
     gene            21305..>21565
                     /locus_tag="VIN32_02225"
     CDS             21305..>21565
                     /locus_tag="VIN32_02225"
                     /inference="COORDINATES: protein motif:HMM:NF018004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HEY8921424.1"
                     /translation="MPTLRPFRGLGYALDRYATPSPPGMRIADLTEVVCPPYDVITPA
                     QQAELLSRNERNAIRLELSAEPDPHAAAAAALKAWQADGTLER"
BASE COUNT         3295 a         7319 c         7621 g         3330 t
ORIGIN      
        1 ggccagggcc tcgcgcagcc accatgcacg gcgttggccg gggatcggca gctcaggtgg
       61 cgcttgggcg gtcacgtcgc gcgatcctaa cccggtgcta gcatccacgc ctgatgcgcc
      121 agcacgatcc agctcggtct ccggtggtcg tcgacgggct gcggacgccc ttcggccgct
      181 acggcggcgc cctgcgcgag gtgaggcccg atgacatggc cgcgcacgtc atccgagcac
      241 tgctcgaacg caccggcgtg gaccccggtg ccgtcgagga cgtgatcctt ggggcggcca
      301 accaggccgg cgaggacaac cgcaacgttg gccgcatggc ggcactgctt gccgggatgc
      361 cgatcgaggt ggcaggccag accgtcaacc gcctgtgcgg atcgggaatg caggcggtca
      421 tcgacgctgc tcacgcgatc actgccggcg acggggacct gttcgtggcg gggggcgtcg
      481 agtcgatgac tcgtgccccg ttcgtgatgg ccaaggcgtc cgcggcattc ccgcgtggcg
      541 agcagacgct ctacgacacg accctcggct ggcgcttcac caaccctcgc ctggccgatg
      601 cctactaccc ctattcgatg ggcgagaccg ccgagaacgt ggtggagcgc tgcggcgtca
      661 gccgcgagga ccaggatcga ttcgccctcc aatcgcacca gcgctgggcg gccgcccagg
      721 cggccggccg ctttaccgac gagttggtgc cgatcgaggc gcccgcctcc aagggagcga
      781 ccacgaccgt ggaccgcgac gagcacccac gtcccgacac caccctggag cagctcgccg
      841 cgctgaagcc cgccttcctg cgcgatgggt ccggatccgt caccgcgggg aacagcagcg
      901 ggatcaacga cggagcatcg gcgttgctcg tcacctccga ggcgcgggct gcggagcttg
      961 gcctgaagcc gatggctcgg ctcctctcca gcgcggtggc gggcgtcgac ccggcaacca
     1021 tgggcctcgg gcctatcccc gcctcgcgta aggcgctcga tcgggccggc ctgtccgttg
     1081 cggacctcga cctggtcgag ctcaacgagg cgttcgcggc acaggcgctg ccggtcatcc
     1141 gagagctgga gctcgacccg gagaaggtca acgtcaacgg cggggcgatc gccatcggcc
     1201 atcccctcgg cgcttcaggc gcgcgcctgg tggcgacgct gctgcacgag atgcggcgac
     1261 ggggcgcgcg gcacggcctt gccacgatgt gcatcggcgt cgggcagggg atcgcatcgg
     1321 tctgggagtc cgtcgagtga acgcatcgga acggacgggc acggtcgatc cgatcggccg
     1381 tccggccggc tggctgtcgc ttggattcgt ggcaggcgtt gtcgcgccgc tcgccgtggc
     1441 ggctgctgcg tatgtcctgt ggtgggtgag cgatcggctc ctctatatcg gaccgttcga
     1501 ccgcgcggcc ttcggctgat ctagccctgg gccaaggctt agcccaagcg cagctgcggg
     1561 agcaccttct ctccgaacgc cgagatgaac tcggcctggt tgcggccgac gttgtggagg
     1621 tggacctcgt cgaaccccat ttccacgaac ttctggatgt gtgccgcgtg ctcgtcgaga
     1681 tcggcggaga tgaggactcg gttggtgaag tgctccggcc ggatcagctt cgccatgttg
     1741 gcgacgtcct cgggattgcg gatgtcctgc ttggggaagg ccatcgcgcc gttcggccat
     1801 tcgcgcaccg cgttcgcctc agccacctgc tgcgtctctg cccacgagag gtggatctgg
     1861 agcagcttgg gcatcccgga tggatccttg cccccgtcag tcgcaccctc ggcgaacttg
     1921 ctccacagcg cgtggatctt ctcgtcggca gcgccgaccg tgatcatgcc gtccgcatgc
     1981 cggcccgtgc gcttggcgtt catcggcccg gccgtggcga cgtagatcgg gaccggttgg
     2041 tcggggcgcg tgtagagctt ggctgactcg agggtgaagt actcgccgtc gtgcttgacg
     2101 acgttgccgc tgaagagctt ggtgatgacc tcgatcgcct cgaacagcat cgcgctgcgg
     2161 atgccgacct ccggccattc gccgccgatg acgtgctcgt tcagcgcctc gccggcaccc
     2221 aggccgagcc agaagcggcc ggggtacatg gctcccaggg tcgccgccgc atgagcgatc
     2281 accgcggggt gatagcgaaa tcccggacag gtcacggcca caccgaacgg caggcttgtc
     2341 cgctgcccca gggcccccat gaagctccac gcgaaggcgc tctgcccctg ctgcggcgtc
     2401 cacgggtgga agtgctcgct gaccatgaag ccggccgaga aaccggccgc ctcagcctgc
     2461 gggcaccatt cgagcagatc gttgggatgg aactgctcga gcatggccgc gtagccgatc
     2521 ttcgtcacgg tcacagccta cccgagtcac cggctctccc gagacgacga ggccgccccg
     2581 cacgagcgag acggcctccg acagagaggg acgagagtca gctggaggag ggctcctccg
     2641 acccgtcggg cttgtcggcg ccaagaccgg ggaatccgcc accgtggcca tggccgggat
     2701 ggagctggga gaggtcgccc gaagccaggg cctggtcgat ctgcgcgagc atcgcgtcgg
     2761 cgcgcgcctg gtcgagacca ccggctacgg cggcgtccag ctttgtcttc gcggcgtcgt
     2821 gaatcgcctt gctgacggcg gcgtagtcct tgccctgcgc cgtcgccacg tccttcagcg
     2881 agtcaccgtt ccgcagcgcc tgcacgagcg cgccgggatc catgcccagg gcgtcggccg
     2941 cggtgctgag cagcggaccg ccgaagtggg ccttgccacc gttcccgccc cccgcgaacg
     3001 gatgaccgaa gaatcggcag gcattgctgc catctgcggc tgcgatcttg tccttcagcg
     3061 cggtggcgcg ctccgcggtc atgtccccgg cggcgaccgc cgcatcgatg gcggcgatgc
     3121 tcgctgcctt tccggccggg aggaggtcgc tgacggagac gccaagctga tcggcgaacg
     3181 tcttctgcca cgtgtcgcag tacgcagcct tgtcagcggg attggctgct gacgcgttcg
     3241 ggttcggcga tgagggcgcc gccagcgcgg cattgatcag ggttccaccc acgagcgccg
     3301 cagccacgag gccgccggcc atgcccaggg tcttgatgtt cttcatgatg gtcctcgttg
     3361 gaatcgtcgc ttgctgcgat ctcggttcgg cggagcatcg gctccggcga tcaagaacgc
     3421 atcaagatct ggctccaggt cttgacatcc tctgaatcag gctgccacta gcatgcgcca
     3481 atggtgcacg ccaggatcct gatcgtcgag gacgagccgc agctgcgcgg gctgctgcga
     3541 ctgtacctcg agcgagccgg ctacgccgtg accgatgccg gtgatggcgc cgcggcgctc
     3601 gccgccttcg cggccgatgg agcggacctg gtggtcctgg acctgatgct gcccgggatg
     3661 cagggcgagg cggtgctcga ggccttgcgc gaagcggcgg acgtcccgat cctgatcacc
     3721 agtgccaagc gctccgacgc cgagcgcatc gccgggcttc gcctcggggc cgacgactac
     3781 ctggccaagc cgttcaaccc ccacgagctg accgctcgcg tggccgccat ccttcggcgt
     3841 tcacggcccg ccgcagccga ggggaccgag gccatcatct cgctcggggc cgggcgcctg
     3901 gtcctggatc cgagctcgag acgctacacg ctgtccggcg gcgagggtgg ccggctgacg
     3961 cccggggagt ccgccctgct cgtggcgctc gctcgacgac ccggcgcggt cctggcccgg
     4021 gaacagctgc tcgaggcggt ggcgcgccgt ccggaggagg tctacgagcg ggtcgtcgac
     4081 gtccacgtcg ccaacctgcg tcgcaagctg ggcgatgacg ccaccgaccc atggctgatc
     4141 gagacgatcc cttcgaccgg gtatcggctg gtggcagcca gggatccggc gtgaccgtcg
     4201 gccaaagcga aatccgcggc cagcctctcg gcctgcggct ggcgctgctc ctgtcggcag
     4261 ccgtgctggc ggtgcttctg atcgccggcc tggcggtgaa ccgcgtcgtc agtggttcgc
     4321 tggagcgaga gctgagcgca gcagagcgtg accgcatcac cgtcgtggcc ggagcgatcg
     4381 ccggcgttga cctgaacgcg ccgaaggcga tgcacggggt cgaggtcgtg ctccgtcgtg
     4441 tcgcgaacac catgcgcggg cacgtgcagc tgatcggccc cgctggcgac ctcctggtcg
     4501 acgtcggacg cctgccacct ggagtctcga ccgagaccat ctcggagccg atcacggggt
     4561 caggcgcaac cctgcggatc gacgtgccgc gtgcggatcg cgcgttcctg ggcgtcttca
     4621 acctgagcct gctcgtcgcc ggcctgctgg cgacccttgc cgtcgggctg atcgcgttgg
     4681 ttctctcttc gcgccttacg cggcccctcc aggcagtggc ggccgcggcg cggcggctgg
     4741 gccacggcga cctcgacgca cgcgccactg gcggccccga ccgggagtcg aacgagctgg
     4801 ccgatgcctt caacgccatg gcggggcgag tccagcgctc cgagatgctg cgccggcgcg
     4861 ccgccagcga catggcgcac gacctggcga cgccggccac cgtcctcgag tcgcagctgc
     4921 aggcgatggt cgacggagtg gtgccggcgg atcgcgaaca gctcgatcgc gcccgcgccg
     4981 ctgccggggc gctctcgggc gtgatcctcc agctgggcga gctggtcgac gccgagtcag
     5041 ccgtcatgca gcgccgcccc cgggtcatct cgctcggcga gctcctggcc gaggtcgagc
     5101 gggcgatcgt gccgctcttc cgcgagcgag gggtcgacat cgaggttgcc gaggtgcccg
     5161 cctcgctcag ggtagacgtc gactccaccc aggtcgggcg cgcgctgcgc aacgtcctcg
     5221 ccaacgccgc ccagcactca ccccgggaag cgactgtgca cgtcgccgtc gaggtggcgg
     5281 ccggcgaggc gctggtccgt gtcaacgacg ccggccccgg gatcgccccc gaggacctgg
     5341 cacacatctt cgagcgcttc tatcgggcgg accaggcgcg gggcgccggg gagccgcgca
     5401 gcggcagcgg gattgggctc accatcgcgc gggagctcct ggccgcaaac ggcggccgca
     5461 tcgcggtcga gcgcacgggc cttgacggga cgaccttcgc gataggcctg ccactcgctg
     5521 gctagggccc ggccagctcc caactgcgct tgcggggcat cgccagccaa tcctctcgga
     5581 ggatcgacat gacgtgcacg tcgtggtgcg caccgcgatg gaagcgtgcg cggcgttgga
     5641 tggcatcggt ctggaagccc gccttctcgt acgagcggac agcccgttcg ttgaagtcga
     5701 agacctcgag ctcgatccgc tccagtcgca gctcgccgaa gccgagatcg acgagagcgt
     5761 tgagcgcatc ggttccgtag ccctggccca ggtagcggcg gtcaccgatg aagatgccca
     5821 cgatcccgct gccgttgacc ttgtcgacgt tgcgcaggaa gacggtgccg atgctctcct
     5881 cgctgcccag caggcagatg gcgtagccgt agccgtttgg gtccatcggg ctctgggaga
     5941 agatctcggc gctgaagcgc tcggcaccgg ccttcgacat cggcgtcttc gctcccagga
     6001 agtggccgac ctcggcatcg cctgcatagg tcgagtcgtg gatgatgtcg gacggttcgc
     6061 tggggcgcag ccagaccttc tcgccccgga tcatgggacg cggcgctggc gcctcggcat
     6121 cgctcatggc gggcatgata ccgacgcccg cggtccggtt gcgcaccccg ttgaggccgc
     6181 gtaccgtggc cgccaccatg gagccctcgt ccgaagccag cctcgaagcg tggtacagcc
     6241 gcttcccgga gggttggtgg ggctaccact ggagcctgcc gcgaccgatg agcgccgtcg
     6301 agatcgttgg cagcggcacc ctcgacgctc gcctgatggc gaccctctgg gccgtgacca
     6361 gccgcaggcg gagcgtgatg ctcgcctctg aggcgccgat ggccggcaag acgacgaccc
     6421 tctcggcatt ggtcgacttc ctgccgcccg gcacggtcgg ggtcttcctg cgtggctggc
     6481 gcggcgactt cgactggacc gatgagatcg gtccagagcg cggttacctg ctggtcaacg
     6541 agatgagcga ccatctgccg atctacgtct ggggccgcaa tgcccgcggg gcgctggagc
     6601 tggcggggcg aggctacggc atgggcgcca cgatgcacgc cgactcgctg gacgaggcgg
     6661 tctccgtcct gcgcgaccag ctcggcgcca ccgatgccga ccttggtggc ctgaccatct
     6721 acctgcagta ctccgcctac cgcacgccgg ccggcatgta ccgtcgcgtc gaggaggcgt
     6781 ggcacctgcg gctggacgag ggcgggacgc tggctcccgt ccggctggca gcgttggacg
     6841 gcggccgctc accgagcctg accggcgcgc agcgcgatcc tgagccgcca ttgctggcca
     6901 gtgacggagg gcgctccagg cgccggctcg tccacgaccg cgaggcgtac gctgtgctcg
     6961 ccgcaagcct ccgtctcgtg ccggacgagc tggaggcgga gctggcggcg cgttccgaat
     7021 acatcgccgc gctggcggac cgcggtgtct gcgatccagc ctccgtcgcg gccgccatcc
     7081 cgctctatcc gtcgctgccc gaaggagcca ccgcatgacc gaccgaccga ccatcggcac
     7141 tctcggagag ctgcgcgccg gtgggtatcg atcccggagc gtcaaggagg agatgcgcgc
     7201 caacctggtg gaccgcctgc gccgcggaga gtcgatgctg ccggggatca tcggctatga
     7261 cgagaccgtc ctgccgcagg tggagaacgc catcctggcg gggcaggaca tcatcctgct
     7321 cggggagcgc ggccaggcga agagccgcat cgcccgatcg ctgataggtc tgctcgacga
     7381 gtggatgccg gtcgtcgccg gcgccgagct gcacgacgat ccgctgcggc cggtcagccc
     7441 gtttgcccgc gggctggtgg cggaacaggg agacgcgacc ccgatcgcgt gggtgcctgc
     7501 cgcggatcgc tacggcgaga agctggccac gccggacatc accatcgccg acctggtggg
     7561 ggaggtcgat cccgtcaagg tcgcggaagg gcgctacctc tccgacgagc tgacgatcca
     7621 cttcgggctc ctgccacgca ccaaccgcgg catctttgcg ctcaacgagc tgccggacct
     7681 ggccgagcgg atccaggtcg gcctcctcaa cctgatggag gagcgggacg tccagatccg
     7741 gggctacaag gtgcgtctca acctggacct gttcgtggtg gcctccgcca acccggagga
     7801 ctacaccagt cgcggccgca tcatcacgcc gctcaaggac cgattcggcg cgcagatccg
     7861 cacccactac cccgaggacc cggagaccga gatcgccatc atggaggccg agtggctggc
     7921 cttcgacgac gagacggcga catcggtccc ggtgcaggtg ccctcgttca tgaaggagat
     7981 cgtggccgag ctgtcgcagc tggcccgacg ttcgtccgag gtgagccagc gctccggggt
     8041 cagcgttcgc gtcaccatcg ccaatcacga gacgctggtg gccgccgccc tgaagcgggc
     8101 cgtgcgcctg ggcgagccga tcggcgcacc acgcgtcagc gacctcggcg cgatcgtggc
     8161 ctcgaccgcg ggaaagatcg agctcgagac gcttggcgac gagactcgcg aggagaaggt
     8221 gatcgagaag ctcgttcagc gcgcggtggt caacgtcttc aaccggcact tctcgctctc
     8281 cgagctggtg agcctggtcg agcgtttcga ggctggcgcg gaggtcgagg tcggcgacgc
     8341 ggtcgcctcg gaccagctgc agcgaacgct cgccgagctg ccggagctga gccgcgccgt
     8401 gggccgcctg gacatcggcg aatcgccggc cggcgtcgcc agtggggtgg agttcgtgct
     8461 cgagggattg cacctcaacc gccggctgaa caaggagcgg cgtggcggca gcgtgcgcta
     8521 cgcacgctag ccggtgagca gcttccgcta ctcgcactgg gacggcagcc agagcctgcc
     8581 tcccttcgac gcggacgacg tgctggaggc gctctccgac gacatcctgg ccgagggcga
     8641 catccggcgt gccctgcaac gactgatgca gcgcggaatg cgcggcacgc gtggcggtga
     8701 cgtccccggg ctgcgacgca tcgtcgagca gctccgcgcg cgccgcgccg aggagctgtc
     8761 gcgggcgaac ctcgacggca tgctcgacga cctcgccgga cggctggagg agatcgtcga
     8821 gcaggagcgg gcggggatcg agcggcgcgt ccacgaggcg gagcagcgag cccttgatgc
     8881 gccacccgga ccggcccagg accaggcgcg gatggcggag caggtgctgc gccgcaccgg
     8941 ccagcagcgg cgcgatcggc ttgacgccct gccggacgac cttgccggcc gtctcgccgg
     9001 cctgcgcgac tacgagttca tggatgccga tgcccgtgac gccttcaacg ccctcaccga
     9061 cgagattcgc cagcagctgc tgcagaccta cttccagggg ctgaaggagg gcgtcgccgg
     9121 catctctgcc gaggatctcg acggcgtgcg ggcgatggtc cgcgacctga acaagctcct
     9181 cgagaagcac gcggccggct ctgacacgag cgcggacttc gccagcttca tggtccgcca
     9241 cgggcactac ttcccgccgg gaatcgaaac cgtggagcag ctgatcgatc acctgcacca
     9301 gcaggcctcg aagatggcat cgctgatggc gtcgttgagt cccgagatgc gggccgagct
     9361 ccagcagatg atggacgagc tgctgcgaga cgatcgcctg cgctgggaca tggcgcgcct
     9421 ggccggcacc ctgcaggccc ttcgtcccga tacccctttc ggcgagccct atccgttcag
     9481 cggcgacgag ccgctggggt tgcccgaggc gctcgccgcc atcgaccggc agcagcaatt
     9541 tgacgccatg gaggaggagc tcctggcggc ccgcgacccc gactccctcg agcagatcga
     9601 cgaggaggcg ctccaggcgc ttggtggtga cgaggccgcc ggcgatctcg agcagcttcg
     9661 agcgctgacc cgcgctctcg aagaggcggg ctacctcgag cgcggtgacg atcggctcga
     9721 gctgacgccg cgagcggcac gcaagctggg gatgcgggca ctgaccgaca tcttcagccg
     9781 gctccggcgc gagtcgctgg gcggccatgc gctgccaacc tcgggcattg gcggcgagca
     9841 gaccgacgag accaagccgt tcgagcatgg cgatccattc tcggtcgacc tcaacaggac
     9901 cctcttcaac gccatggcgc gcaatggtcc cggcaccccg gtccggatcg cgccggccga
     9961 cttcgaggtg catcggtcag aggagacgac ggtcagctcc accgtgctgc tgctggacat
    10021 gagccgatcg atgctgctgc gcggctgctc gaccgccgcc aagcgggtgg cgatggcgct
    10081 ccacacgctg attcacacca agtacccccg cgatcggctg tacgtggtcg gcttcgcgta
    10141 ctacgcacgc cagatcaagc cggaggcgat cgccactctc tcaccgtacg agttcgagta
    10201 cggcaccaac ctgcagcacg cgctgatcat cgcgcgccag ctgctcggcc gccgcagcgg
    10261 cggcaacaag gagatcgtgg tgatcaccga cggcgagccg acggcgcata tcgccaacgg
    10321 ccaggtcgag ttcgcctacc ctccgacgat gcggaccatg cagtcgacgc tgcgcgaggt
    10381 tgggagggcg actcgcgagg ggatcgtgat caacaccttc atgctggagc ggtcgcgcta
    10441 cctctccgaa ttcgtcgacc tgatgagccg gatcaaccgc ggtcgcgcct tctacgtcga
    10501 gcccgagcac ctgggcgagt acgtcctggt cgactacgtc agcaagaagc gcaagcgggt
    10561 cgcctgatga aggtcggcgt ccagctgccc gaggtggagt gggtggcccg ctggccgcag
    10621 cagcgcgaga tggctcgcac cgccgaggag atcggcctcg actcgatctg ggtcggcgat
    10681 cacctgctgt accgcggtga cggcctgccg tcgcgcgggc catgggccgc gtggaccagc
    10741 ctcgccgcaa tcgccgcggt cacggagcgg gtcgccatcg gccccttggt gacggcggcc
    10801 agcttccata accccgccat gctcgccaag caggccgcca cggtggacga gatcagcggc
    10861 ggacggctga tccttggcct cggcgccggc tggaacgagg cggagtacct ggcctacggc
    10921 tttccgttcg accatcgagt gagccgcttc gaggaggcgt tcacgatcat tcgcacgctg
    10981 ctgacggagg gccgctgcga cttccacggc acctactacg acctgcagga ctgcgagctg
    11041 ctgccgcggg ggccgcgcca cgcccagggc gaggggccgc cactgctggt cggctcgatg
    11101 ggggagcgga tgctgggcat cacccttccc tgggtgcagg cctggaacgc ctggttcacc
    11161 tggtttggca acacggtcga gggctaccgg ccgctgcgtg acacggttga tgcggcatgc
    11221 cgtgcggcgg gccgcgaccc ggcggaggtg gagcgcacgc tggcgctgct ggtggccttc
    11281 ccgggcgcaa agggcaggac actcggcgac ctgaaggagc cggaggtcga gccgatcccg
    11341 agcaatcccg ccaccctggc ggcgaccctc cgcgatttcg aggcggaggg cgtggcgcac
    11401 gtccagctgg tgctcgaccc gatcaatgcg gagacgatcg ctggcctcgc cccgatgctg
    11461 aaggagctcc gcgcctagat cgcggggcgg tatgctcggc gcatggaggg tccccccgcc
    11521 cggttaccgc tcggcccgaa cggtcggctc gaggctgagc cgctgaccaa tgcggttggc
    11581 aatcacgccg tcctggtgcg ggaaggcgag cagccgcgag cactcgtgac cgtcgaggcg
    11641 ggcagcccgg agagacagcg ggtgatcatc gtgccgctcg acaacgacgt cgagatgcgg
    11701 gtcgacggcg acgccctcgc catctggctg tcgcagggct cgatcggccc ggcgccctcc
    11761 gacgtgcctg gctgggccaa cgccatcggc ttcgggctgg tgatcgccgc cgtcggcttc
    11821 acggtgcttg gcagcgtcac cttcttcggc tggctgttcc ggacgctcgg atggctctga
    11881 ccccacccac ctccgggctg atcgcgatgc gcatcggcca ggagttcggc ccgccggagg
    11941 agttcgagcg ctcgctcgag cgagccatcg aacgcggggg cgagcgggga gcgacgatcg
    12001 tcgcggtcct ggacctcggc gacctcgcga cccacatccc gcaggtcgac ggcccgtcgt
    12061 ggaacaccgt cccactcgtg cacctgcacc gtggacagca gccgaccgag gaggactggg
    12121 cggtcgccaa tgccatcgtc gagcggctgg agcgctaccg ctgagccgat ggacgaggat
    12181 cttctcctcg ggttgcgggc gatgatgggc cacttcgcca ccggcgtgac ggtggtcgcc
    12241 gcgcgccacg ggccactgct ggccggaatg acggcgaacg ccatcgcctc gatcagcctt
    12301 gacccgccgc tgctgatggc cagcatcaac cggggaagcg agacgcacgt ggccatcgtc
    12361 ggaagccact cgttcgccgt cagcgtgctc ggtgccgggc agcgcgcgat cgccgactgc
    12421 tttgccctgc cgaccaccgc cggaaagctt cagcgcttct gcgacgcgcc gtggcacgag
    12481 gcggagaccg gctcaccgat cctcgacggg gcgatcgcct tcttcgactg tcggctgcac
    12541 gaatcgcatg ccgcgggcac gcacaccatc ttcatcggcg agatcgtggc cgcggggtac
    12601 gaggagggcg gcgagccgct cctctggttc gggtcgcgct atcgccgcat cgcggccgaa
    12661 ccgggcgagg gctaggcccc gaccgcggcc agcgactcgg agatgacgcc gatggcggtc
    12721 tcgacctctt catcggtcgt gaccagaggc gggatgacgc gcaccacctg gccgtaggtg
    12781 ccgcagctga ggagcagcag cttgcggtcg aaggcctcgt gcaggattgc cttggcgagg
    12841 tccggccgcg gcgtcagctc gggcccctgc gcgagctcga tcccgatcat caggcccctc
    12901 ccgcgaacct cggcgacgca gctgtgtccc acggcggccc catgcagccc ggcaagcagc
    12961 cgctctccct ggcgcgcggc gttggccagc agcccctccg actcgatcac gtcgagcgtg
    13021 gccacggccg cggcgcaggc gaccgcattg ccgccatagg tcccgccatg ggcgcccggt
    13081 cccagccggt cgagcagctc gcggcgggcc atgatgccgg agagcggcat ccccgaggcg
    13141 atccccttgg cgatgaccag gatgtcgggg cgcgcgtcgt accagtcggt cgcccacatc
    13201 ctgccggtgc gccccatccc ggtctgcacc tcgtcggcga tgagcaggat gccgtgccga
    13261 tcggcgatct cgcgcagcgc gggaaggaac gagtcaggcg ggaccacata gccgccctcg
    13321 cccaggatcg gctcgaccag gaaggcggcc acgtcatcgg gatagatgac ggtcgcgaag
    13381 agctcctcga tccccgccag gctgaaggcc actgccgcct cggcgttgcg gccggtcggg
    13441 ttgcgcagcg cgtacgggta cggggcgaag tgcacgccgc ccatcagcgg ctcgaagtgg
    13501 cctcggtact tgacccccga gctggtgagc gccatggccc cgtgcgttcg gccgtggaag
    13561 ccgccgcgaa aggcgatgat cgccgggcgc cgggtggcgt acttcgcgag cttgaccgac
    13621 gcctcgatcg cctccgcccc cgagttggag aggaagaggc cagcctcatc cgcacctctg
    13681 agccccggga aggtgcgggg caggcgcgcg tggagctcga ggccgggctt gtgatggagg
    13741 atgttctgct gggcgtggat gatcctgccg gcctgctcgg cgatcgccgc ggcaacccgc
    13801 gggtgggcgt ggccggtgtt ggtgaccccg atcccacagg tgtagtccag gtagcgctcg
    13861 ccctcgatcg tctcgatcca cgagccgatg ccccgttcga ccaccaggtc ggtgtagcgg
    13921 gcccagaccg gcggcacgcc ggcgcggagc tcctgccagg gagtcgcttc gtcggtcatc
    13981 ggcttgccac cggcgcgatc ggcaccccgg tctgctgcgc gtcccggcct ccccgacgct
    14041 gcgcccagac ctggacgatg atgaagacga aggcgatcag gaagaagagc gtgcccagtg
    14101 cattgacctc cggggggatt ccgatgcggc tggccccgta gacccacagc gggaacgtga
    14161 cggtatggcc ggcgctgaag agcgtgatca catagtcgtc gatggagagg gcgaacgcca
    14221 ggagagcggc agccaggatc cctgggaaga tgagcgggaa ggtcaccttc cagaaggtgg
    14281 tcgattccgt ggcgtagagg tccatccccg cctcctccag cgagcggttg aagccgatca
    14341 gcctggctcg cacggtcacc acgacgtagc tgatgttgaa catgatgtgg gccaccagga
    14401 tcgtcggcag gccgcgcccg aggccgattg ccacccacag agtgaggagc gaagcgccca
    14461 ggatgacctc cggcgcggtc atcgggatga agatgagcag gttgaggatg ccccgcccgt
    14521 ggaactgata gcgggtcagc gagagggcga tcatcgtgcc gaagacggtc gccacgatcg
    14581 tcgagagcac ggcgatggtg aggctcgtca ccatcgggtc ggtgatgtcc gagcgattcc
    14641 agatggtcag gtagttgtcg agggtgaagc cctgccaggt gatgttctgg tgcccacgcg
    14701 ggctgttgaa gctgaaggcg atcatcacca cgatcggcgc aaacaggaag gcgacgacca
    14761 gcgcggtgaa ccccggcagc agccagcgcg tcaggcggtg catcagctcg tcagccgatc
    14821 ggtgccgacg agccgcgtat agacgcccac cagcagcatc gtcgccgcca cgagcacgaa
    14881 gccgagggcg gccgcggcag ggaagtcgtt gacactcagg aagagccgct ggatcacgtt
    14941 gccgaccatc gcgacctccg ggctgcgggc cccgatgatg tcggcgttga tgaagtcgcc
    15001 caccgcggga atgaaggtca gcagggagcc ggccacgacg ccgggcaggg cgagtgggaa
    15061 ggtgatgcgc cagaaggcct gcgcccgact ggcatacaga tccgtgcccg cctcgatcag
    15121 gcggcggtcg atcttctcca gcgcgacgta gaggggcagc accatgaatg gcaggaagtt
    15181 gtaggtcagc ccgaagatca cggcggcggg agtggccagg aagcggaacg actgctccag
    15241 gaggcctgcg tccttgagcg tgccgaagac gagcccgttg tcggagagga tcatccgcca
    15301 gttcagggtg cggatcacga agctcacgaa gaacggcatg atcacgacca ggaccaacgc
    15361 gttcttgagc cggccgccgt aaaaggcgat ggtgtaggcc atcgggaagc cgatcgccag
    15421 ggtcaggacc gtggtcgcca gcgcatagct gacggaacgc acgaagagca ccccgtaccg
    15481 ggaagccacc tcggggtaga tgccccagtt ccaggtcagg tggtagccgg tgctcagcga
    15541 cccttcctgc agcgacatga ccagcatgaa ggccatgggc acggcaaaga agaggaggag
    15601 ccatgccgta cccgggagga ggagcaggta cggcaccagg cggcggccgg cgcggttcat
    15661 ccggctgcgg tcgtgacctc caggaacatg tcgttccagg ccttctcgtc gtccggattc
    15721 tcgaactgcg gatagtcgaa cgtcccggcc agctggtccg gggttggcgt gacggtcggc
    15781 cccacggcgt cgtagtagtc ggcgttctcc gtgtcgccat cggcccggta ggcatcggcg
    15841 tcggccttga tcagctccga gacgcccttc accggggtga agtagccgac gtactccgag
    15901 agcgtggtgg ctgccgccgg gtcgtaccag tagttgatca gcttgtgcgc ctgatcgatc
    15961 caggcggcct tcttggggat gaccaggttg tcattccagc gcggggcccc gtccgacggc
    16021 acgacgaata ggaccttgtc gttgtcgttg agctgcatct gggtgatgtc acccgaccag
    16081 gcgaccgatg cgaccaggtc gccggctgcc agcgcgtcgt agtactcatt gccatagaag
    16141 ccgcggaact gcccggcctt ggccgccgtg agcagcttgt ccctggcctg ctgcgcctga
    16201 gcgacagtgg cggtggaggg gtcgatcccg agggacgcca ggcagagggt catcatgtcc
    16261 cgcatgtcgc tgaatccgcc gacccgaccc ttgaactcgg ggtcgagcag gtcgtcgaac
    16321 ttggtgatct cgcggccggt cagcttcgga tcgtagccga tgccggtgat ccccccctgc
    16381 caccacacgc tgtacttgtt gtcggcatcg aaccagaggc ccttggcata atcggcgcag
    16441 ttcgccgtcc agttggggag cttgctgtgg tcgatctcct ccaggtagcc ctggtcggcc
    16501 atccgcttca ccatccagct ggttgggctg acgatgtccc agtcggtggc gttgccggca
    16561 gccaggtccg gctggatggt cccgtagaac tcctcgttgt cgttgatcgc ctccacgtag
    16621 tggacgtcga tccccgtgtc cttgatgaat ttgttgaggc tcggatacgg agtcggggcc
    16681 ggcgcggcct tgtcgtcgat gtcgatatag aggggccagt tggcccattc cagcttcttc
    16741 ttggccggcg cgcaggcggc aagcagcgac gggagcgtca gcatcgcccc gagcgcgagt
    16801 ccgccacggc ccgttctgcg catgaacgtg cgccgcgaca tggggtcctg gcgcaggatc
    16861 ctgtcgatgg cgacctcaat ctcgcctcgc tcctcggggt cacgcatggg actctccttc
    16921 ctccgtcagg tgcggatcgc cggcgacatg ctcggcgggg ccgctgatca cgaacgtgtg
    16981 ctgtggcttc caggtcagtt gcacgtgctg gccgcctgcc agcagctcgc cggctccggc
    17041 ggtgtcgagg ttctgggcat agaccgtgag ccgatgcccc tcggcggtct gaacgaggta
    17101 ttgggtgctc accccgatgt agctggcgtc cagcacggtc ccgctcagcg cgttcaggcc
    17161 gttgctggcg ccggcctcga cgcgagcatc gacccgcagc ttctcgggcc gcacgccgat
    17221 ccggaccggt ccggtggtcg ccacgccctc gccgggggcg cgcagaaccg tcccgtcgtg
    17281 gagcttgacc gtgacgagtg agccgtcgcc gccggcgatc tcaccctcga gcaggttgga
    17341 gacgccgagg aaccccgcca cgaaggccgt gcgcggccgc tcgtagagct cctcggggtt
    17401 gccgagctgc tcgatccggc cggcgcgcat gactgcgatc cggtcggaca tggtcatcgc
    17461 ctcttcctgg tcatgggtga cgtagatgaa cgtgatccct acctcggtct ggatccgctt
    17521 cagctcgagc tgcatctgct tgcgcagctt caggtcgagg gcgcccaggg gctcgtcgag
    17581 aaggaggaca cgcgggcggt tgatgagcgc gcgcgcgagc gccacgcgct gctgctggcc
    17641 gcccgagagc tgagacggct tgcgggcctc gtagccgggc agctcgacca gctcgagcat
    17701 cgccccgacc tggttcgcga tctcgctggc ggccaccttt cgccggcgga gcccgaacgc
    17761 gacgttctca aagacagaga gatgcgggaa gagcgcgtag ttctggaaga ccgtgttcac
    17821 gtcgcgcttg aatggcggca gcccggtgac atcggcgtct ccgaggtaga tcgtgccgga
    17881 ggtcgcctcc tcgaagccgc cgatcatgcg cagggtggtg gtcttgccgc agcccgatgg
    17941 gccgagcatc gagaagaact cgccacgctc gacctccagc gagaggtcgt cgaccgcgac
    18001 gacctcgtgg aatcgcttcg tgatgtggtc gaggcgaacg tcgacgcttt gcggcaaccg
    18061 gggtccctcg tgcgaagtgg ggatcctgat cggagaaggg cggccgtgcg ggccagatcg
    18121 gagtatagga ccccgtcgcg cggcggtgtc aacgagcggg gctgctagac tcgcccgacc
    18181 gatggacttt gccctctcag cgccgcatgt cgagattcgg cgcaccgttc gcgacttcgc
    18241 cgagcgggaa atcgaacccg tcgccgatga gatggagcgg agcggcgagt tcccggcgga
    18301 gatcatccgc aaggctgccg cgctcgggct gctgggcgtg ccctatcccg aggaggtcgg
    18361 cggcaccggg ctcgactccc ttgcctacgc catcacggtc gaggagctgt cgcgcgtctc
    18421 cggcagcgtc ggcatcatcg tcagcgcgca caccagcctc ggctgcgcac cactgttcac
    18481 ggccggcacg ccggagcaga aggagcgata cctgcgcccg ctggccagtg gcgccaagct
    18541 cggcgcctac ggcctgaccg aggcgggagc gggctcggac tcgcgcggga cgcggacacg
    18601 ggcgcatcgc gacggcgagg actgggtgct gaccggtagc aagcgcttca tcaccaatgc
    18661 gggggtggcg gacacctaca tcgtcaccgc cgttaccgat cgaaacgccg actccggcaa
    18721 gatcagcgcc ttcatcgtcg aggccgggac gcctggcttc tccatcggtc ggatggagga
    18781 gaagatgggc ctccacgcca gcaacaccgg cgagctgctc ttcgacgact gccggatccc
    18841 ctccgaaaac ctgctggggg aggagggcga cggggatcgg ctcttcctga agaccctcga
    18901 cggcggcagg atcgggatcg gcgccatggc cctgggcctc gcccaggcag cgtacgaggc
    18961 cgcctcggcg tactcgaagg accggcagca gttcggccgg ccgatcgcct cgttccaggg
    19021 gatcgccttc aagatcgcgg acatggcgac ccagatcgac gccgcccgcc tgatggtcta
    19081 ccgcgccgcg tggctgaagg acagcggtgc gccgtattcg accgaggccg ccatggcaaa
    19141 gctcttcgcc agcgaggtgg cgcgccaggt gaccaatgac gcggtgcagg tccacggcgg
    19201 ctacggctac atcaccgagt accgcgtcga gcgctacctg cgcgatgcga agctgaccga
    19261 gatcggcgag gggacgagcg agatccagcg catggtcatc gcacgcaacc tgctcggcgt
    19321 gcgcgcgatg tgagcggtcc gctcttcggg accgataccg acgccgcggc ctggcgcccc
    19381 tcgccggcat acctgcagcg gagccgcctc gcccggttcc tggccgccac cggctccgcc
    19441 gacctcgaag cgctccaggc ccgcgccgtg gcggatcccg cctggttctg gcaggccgcg
    19501 gtggcggata tcggcgtccg attcgacccg gagccgggca ccgtgctgga taccacccgc
    19561 gggatcgagt gggcccgctg gttcagcggc gccggcttca actacgtgcg catggcgctc
    19621 gacgagcccg cggctgctcg tccggacgag atggcgctcg tctgggaggg ggaggacggc
    19681 gaggtccgcc agttcacccg cgcgcagctg cgctgggcgg tcgaccgcgc ggcacacgcc
    19741 atggtcgcgc tgggcgtgga gcgcggcgat cgggtcggca tcttcctgcc catgattccc
    19801 gaggcggtga tcgccgtgct ggcggtgggc aagctcgggg cgatctacac gccgatcttc
    19861 tccggctacg gagccgatgc ggtcgccagc cgcctcgccg actgcgaggc gaagctcctg
    19921 gtcacggccg acggcttcgc gcgtcgcggc aagccggtgc cgatgaaggc gaccgccgac
    19981 gaggcattgg cccgggcttc atcggtggag cgctgcctcg tcgtccggca cgccggaggc
    20041 gaggtcgcct ggcgcccgga gcgcgacgtg tggtgggacg cggctgtgac atcggcctcc
    20101 gatcagccgc tggagagcgc ggcgaccgac gccaacgacc ccttcatgat catctacacc
    20161 tcgggcacga cgggccggcc caagggggcg ctccacgtgc acggcggctt tccgatcaag
    20221 gcggcggtgg acctggccca ctcattcgac ctgcaggcag gcgacacgct cttctggttc
    20281 acggacatgg gctggatgat gggtccgtgg gccgtcgccg gggccccgat gctgggcgcc
    20341 acgctgttca tctacgaggg aacgcccgat tacccgggtc ccgatcggct gtggggcctg
    20401 gtggagcgcc accggatcac ccacctgggg atcagcccca cgctggtgcg cgccctgatg
    20461 acccacggcg acgagccgcc cggagggcac gacctgtcga cgctggtcgt gctcggctcg
    20521 acgggcgagc cgtggaaccc cgatccgtgg tggtggctgt tcaagaccat cggaggctcg
    20581 cgcctgccga tcatcaacta ctcgggcggg acggagatca gtggcgggat cgtgggctgc
    20641 accaccattg cgccgatcgc cccctgctcg ttctccggtc cctgcccggg aatggcggct
    20701 gacgtggttg acgccgaggg acgccccgtg cgcgacgagg ttggggagct ggcgatccgc
    20761 cagccgtggc ccggcatgac gcagggcttc tggcacgatc gcgagcgata cctggaggcg
    20821 tactggagcc gcctgccggg ggtgtgggtg cacggcgact gggcgcgcgt cgacccggcc
    20881 ggcttctggt acatctccgg tcgctcggac gacaccatca aggtcgccgg caagcggatc
    20941 ggtcccgcgg aggtcgagtc ggccgccgtc tcccatccgc tggtggccga ggcgctggcg
    21001 ataggggtgc ccaacgagat caagggcgag acgatcgcgc tcttcgcagt tgttcgcggc
    21061 gacgctgacc ggggcgacgg gctggcggcg gatatcgggc agacggtggt cgagcggctc
    21121 ggaaagtcgt tcaggccggg agcggtgcac ctggtcccga gcctgcccaa gacgcgcaac
    21181 ggcaagatcc tgcgccggct ggcgcgtgcc gcctacctgg ggctcgatcc gggcgatatg
    21241 agctcgctcg aggacgcctc cacgctggaa ccgatacggg cgctgggcgg cccctagcct
    21301 ccgcatgccc accctccgcc cgttccgcgg gctcggctat gcgttggatc gctacgcgac
    21361 cccctcccca cccggcatgc ggatcgccga cctgacggag gtggtctgcc caccgtacga
    21421 cgtcatcacg ccggcgcagc aggccgagct gttgagccgc aacgagcgca acgcgatccg
    21481 gctggagctc tccgcagagc cggatccaca cgcggccgcg gccgccgccc tcaaggcctg
    21541 gcaggccgat ggcacgctcg agcgg
//
LOCUS       DATCQR010000065        12817 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_204183, whole genome shotgun sequence.
ACCESSION   DATCQR010000065 DATCQR010000000
VERSION     DATCQR010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 12817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12817
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204183"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..877
                     /locus_tag="VIN32_02230"
     CDS             <1..877
                     /locus_tag="VIN32_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="proline racemase family protein"
                     /protein_id="HEY8921425.1"
                     /translation="PDCDAGFIIMEPTEYPPMSGSNTICTATVLIEEGLVPVTEPITQ
                     LTLEAPAGLIRVRAEVRDGRATSITFENVPAFAIEVNAPVEVPEIGRIKVDVAWGGMF
                     FAIVEASALGLGLTPDEGRDIVRLGEMVKAAAREQIPVAHPDHPKIRGISIVELTAPP
                     TLPGAHGKNTVVVSSGTLDWSRPESFTGVLDRSPCGTGTCARMAVLHARGKLGIGEDY
                     VHEGILGTAWTGRLLRETTVGPYRAVVPQLTGSAWITGRAEYVVDPDDPFPEGFTVGD
                     LWGLTEEGSSPTAIS"
     gene            962..2053
                     /gene="ugpC"
                     /locus_tag="VIN32_02235"
     CDS             962..2053
                     /gene="ugpC"
                     /locus_tag="VIN32_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011917850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEY8921426.1"
                     /translation="MATVTFDHLFKRFSGDVIAVNDLSLEIADGEFLVLVGPSGCGKT
                     TALRCIAGLESISEGKLLIGDQVVNDVAPKDRDIAMVFQSYALYPHMSVFDNLAFGLK
                     LRKMPKAEIDTRVREAAGILGLDKYLDRKPKALSGGQRQRVALGRAIVREPKVFLMDE
                     PLSNLDAKLRVQTRAEIARIHRRLGTTIVYVTHDQVEAMTMGDRIAVMKDGLLLQVGT
                     PEELYTHPANLFVAGFIGSPAMNFVTVTTAGGELMMGQTRLSLGGEPARVVAAQIPGK
                     SLTIGFRPEHLKLANGTTDSVMRFPAEVDVVEYLGNEELIHARAEGNDIVALLPSDRK
                     VKAGEQVELAVPLDRLYVFDPTSEKALIN"
     gene            complement(2085..3179)
                     /locus_tag="VIN32_02240"
     CDS             complement(2085..3179)
                     /locus_tag="VIN32_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019637015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEY8921427.1"
                     /translation="MPRRRRWLGQLAVNGTLAVICFIWLVPTIGLLVSSIRPAADVLN
                     SGWWSVLPHQDYVTTKELPIPTGVTGDAPITIEGVSATFDGFRGGVTAPDGRRILWVG
                     NRRTGRIEIQQKQWVAPFNFTLRNYQVVLSGGDKTYENTGGTLRTERGSDLGSAFMNS
                     IAVTIPATVIPLTVAAFAAYGFAWMRFRGRMALFALLVALLVIPLQIALVPVLRDYTG
                     LNLNGTYLGVWLAHTGFGLPLAIYLVFGFVSEIPRDLIDSAVTDGASHFAVFRRIIIP
                     LSVPVLASFAIFQFLWVWNDYLVATTFLGGTQDVQVLTIKLVNLAGTRGQDWQLLTAG
                     AFVSMIIPLIVFFALQRYFVRGLLAGSVKG"
     gene            complement(3182..4294)
                     /locus_tag="VIN32_02245"
     CDS             complement(3182..4294)
                     /locus_tag="VIN32_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEY8921428.1"
                     /translation="MTEPPLAAFNDEAPPEARARNLALGIGGVILTVVFLVAGYVILA
                     DKASPIPKPLITLLAVIWGVGGLLAIFTVSNWIVEQLPARAMQILLPVVFIGPAVIFL
                     VYFLVLPAIRTFATSFFDQDGAAFVGLANYIRVFTDDATLLAFRNNLLWLAFGASLTV
                     IMGLLIAALADRSRFEVIAKSIIFMPVAISLVGAGIIWKFVYAVRDPSSPQIGLLNAI
                     WVGLGHSPIGWLADNQPLNNLFLIVVVVWLQTGFAMVLFSAAIKGVPADLLDAARVDG
                     ANEIQTFMRIVVPTIMPTIITVATTIVIFTLKIFDVVVVLTNGNFGTQVIATEFYTQF
                     FSNRSFGLGSAIAIVLLILVLPVMVYNLRRFRQQQA"
     gene            complement(4434..5810)
                     /locus_tag="VIN32_02250"
     CDS             complement(4434..5810)
                     /locus_tag="VIN32_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009224665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8921429.1"
                     /translation="MRQHWWRGASLLFAGTILLAACSSGSSPSASAAGSTAAQPSGSA
                     ATSSGTKELDDALAGKYTGKVVTAYGPFVDEDQAKYETMVKVFEDATGIDVKYEGTKQ
                     FEQDISVRVDAGNAPDVADFPQPGLLASFAKTGKAVDLSSFLSADYLKAQYVQSWLDM
                     ATMPGPSGNVTAGVWERVNAKSFIWYPKKAFDAAGYKVPTTWQELTDLTAQIQADGDT
                     PWCVGIESGVATGWAATDLINDIMLGSQPPETYDKWVAGTLPFSSPEVKAAWQTMADM
                     WLKPGEVFGGTKGIVSTAFGDAPASMFTDPPKCWLHRQGNFITSFFPKTAVAGVDYDF
                     FPLPAVDASHAGYMQVAGDIWGMFNDRPEVRALLEWYTSAKHLEPWMATGGALAPQKD
                     VDTSKYGQAVEQKIGALLKSATAIRFTSGDQMPGAVRTAFWKEITSFINGDETIEQAL
                     ANIDKAKS"
     gene            complement(5920..7017)
                     /locus_tag="VIN32_02255"
     CDS             complement(5920..7017)
                     /locus_tag="VIN32_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019324287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY8921430.1"
                     /translation="MARESALTRKATAISDRQHHAGRQAVTLDAVARVAGVSRATVSR
                     VVNGNPKVTPVVRHAVERAVGRLGYVPNPAARSLVTRRSGFIALVIPEQTTSLFGDPF
                     FPRLLAGISSELAASNVQLVLMIAQGSAQSRAEAYVAAGHVDGALLASLHGDDLLPDR
                     LRTRGVPVVVGGRPPTSSTVSYVDVDNEVGARTAVEHLLEGRRRRVATITGPLDMPVG
                     VDRRAGYRRALAVAGIVQDDGLEANGHFTQEGGAMAMRAILARHPDVDAVFAASDLMA
                     IGAVGELRRAGRRIPDDVAVVGFDDSALAAASEPPLTSIRQPIEEMGREMTRLLLEAI
                     ETRSPIPKQVLLGTSLVVRESSAATMGMRSA"
     gene            complement(7103..9553)
                     /locus_tag="VIN32_02260"
     CDS             complement(7103..9553)
                     /locus_tag="VIN32_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019638155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HEY8921431.1"
                     /translation="MRYGHFDDSRREYVITRPDTPLPWINYLGSEDYVGIISNTAGGY
                     SFYRDARLRRLTRYRYNNAPLDLGGRYLYLRDDDSGATWSPSWQPMRRDLTDYSCRHG
                     LGYTVIGARFTDIHVETTYLVPLSETLEIWSARITNERETAASLSIFSAVEFCLWDAN
                     DDATNLQRNYSIGEVEVSDGVIYHTTEYRERRNHFAYFACSEPIAGFETQRDAFLGPY
                     RGWEDPLVVERGATTNSIAHGWQPMGSHHVRLELGPGATREVTFVLGYAENPADAKFD
                     PPGSTTVDLRGVRPVIDRYLEPDAADRAMAALRTRWDELLANLQVQTGNEHLDRMVNV
                     WNAYQCMVTFNVSRSASLFESGIGRGMGFRDSNQDLLGFVHMIPERARQRILDIASTQ
                     LPDGGAYHQYQPLTRRGNDAIGSGFNDDPLWLVLAVAAYLKETGDAGLLDEPVPYDNR
                     QGSETALYEHLQRAVGYTLERLGPHGLPLIGRADWNDCLNLNCFSETPGESFQTTENR
                     SGGVAESIFIAGLFVLAASEMREIAARRDDTAEARRCAAAAAAMDAAVDSSGWDGAWF
                     RRAYDYFGAPVGSAENEEGQIFIEPQGICVMAGIGLRDGRAVRALASVRERLATPHGL
                     MLVQPAFTRYHVNLGEISTYPPGYKENAGIFCHTNPWVMIAEAMTGNPDAAVDYYLRI
                     NPSAREEISEIHRTEPYVYAQMIAGRDAATHGEAKNSWLTGTAAWNLVAVSQWILGIR
                     PEHDGLRIDPRIPAEWAGFSVTRHFRGSTYRIRVVKARGAVGRSTRLTVDGADVDGTL
                     APLPRTRDQEIVVEVHID"
     gene            9780..11438
                     /gene="treS"
                     /locus_tag="VIN32_02265"
     CDS             9780..11438
                     /gene="treS"
                     /locus_tag="VIN32_02265"
                     /EC_number="5.4.99.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002811514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047471 - maltose
                     alpha-D-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose alpha-D-glucosyltransferase"
                     /protein_id="HEY8921432.1"
                     /translation="MAPLDGDPLWYKDAVIYQLHVKAFADSSGDGIGDFIGLTGKLDY
                     IASLGVTTIWLMPFYPSPLRDDGYDIADYTNIHPAYGTRADFRAFVRAAHKRGLRVIT
                     ELVINHTSDQHPWFVESHASRTNPRQDWYVWSPDDKKFADARIIFNDTETSNWAWDNA
                     RQEYYWHRFFSHQPDLNFDTPAVLRAIIRVMRFWLEMGVDGLRLDAVPYLVEREGTDG
                     ENLPETHAVLKRLRAAMDERHPGRMLLAEANQWPDEVRPYFGDGDECNMAFHFPLMPR
                     IWIALRKEDRSPIVDIIERTPPIPETAQWALFLRNHDELTLEMVTPDERDYMWNEYAA
                     DLRTRINMGIRRRLAPLVENSRGRLELLNGLLLSMPGTPVIYYGDEIGMGDNVYLGDR
                     NGVRTPMQWTPDRNAGFSGADAAALYSPVIVDPVYGYGAVNVEAQERIPGSMLHWMRR
                     ILGVRRSYPAFGRGSLRFLEPENMRVIAYLREHGGVTILCVANLSRFVQYVELDLREF
                     DGMVPIEMIGHVRFPPIGELPYLLTFAPHGFYWFLLTDGAHAGD"
     gene            11416..12759
                     /locus_tag="VIN32_02270"
     CDS             11416..12759
                     /locus_tag="VIN32_02270"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEY8921433.1"
                     /translation="MARTPAIDRRLQRLASAFTPEWLAAQRWYRAKTRTLAGVELLDA
                     AEIDGSPGWLLVLEAAEAAGGHSRYLVPVVLDRDLFSEPGDGDGVWRALAGLMLDGGE
                     LVGTRGRWSFSPTNAASALQPGGAAALASLAERRLGVQQSNTSVALGEALMLKVYRLV
                     ETGANPEVEMNAFLTEAGFHEAPALAGSATYLLDGEPHSAAMLQQLVRSAGDAWSWVL
                     ERVTSEPDGAAEALRGIAQIGNLTAGMHAALASRPTAPGFPSRVATWEELSMWSSSAR
                     RQLSDALKVLDETTRPRLAKIVPRITAGLGAIQDAGQVRVSRIHGDYHLGQLLRTDDG
                     FTVIDFEGEPARTLAERRAPASPLRDLAGMLRSLDYAAQAAVAGYSSPGTNSEWLLAA
                     REAFIGGYGGIRADQATLLAAFELEKACYEVVYEANNRPDWTWLPLGALERLATG"
BASE COUNT         2205 a         4231 c         4287 g         2094 t
ORIGIN      
        1 gccggactgc gacgccggct tcatcatcat ggagccgacc gagtacccgc cgatgtcggg
       61 gtcgaacacg atctgcaccg cgaccgtgct gatcgaggag gggctcgtgc cggtgaccga
      121 gccgatcacc cagctcaccc tcgaggcgcc agccggcctg atccgggtgc gtgccgaggt
      181 gcgcgacggc agggccacct ccatcacctt cgagaacgtg ccggccttcg cgatcgaggt
      241 caacgccccg gtggaggtgc cggagatcgg caggatcaag gtggacgtgg catggggcgg
      301 catgttcttc gcgatcgtcg aggcatcggc gctgggtctg ggcctgactc cggacgaggg
      361 acgcgacatc gtccgtctgg gcgagatggt caaggccgcc gcccgcgagc agatccccgt
      421 ggcacatccc gaccacccga agatccgagg catcagcatc gtcgagctga ccgccccgcc
      481 gacccttccc ggcgcacatg gcaagaacac cgtggtcgtc agctctggca ccctcgactg
      541 gtcgcgcccg gagtcgttca cgggcgtcct ggaccgatcc ccgtgcggga ccggcacctg
      601 cgcccggatg gcagtgctgc acgcgcgagg caagcttggc atcggcgagg actatgtcca
      661 cgaggggatc ctgggaacgg cctggacggg ccgcctgctg cgcgagacga cggtcggccc
      721 gtaccgcgcg gtcgtgccgc agctgaccgg cagcgcctgg atcaccggca gggccgaata
      781 tgtcgtcgac ccggacgacc cgttccccga gggcttcacg gtcggtgatc tgtggggatt
      841 gaccgaggaa ggctcttccc ccacggccat cagctgacat atgatggccg cccgcgcccg
      901 aagcgcggcc aggttgaccc gcccgatggg cgagcgcaga cccctcaaga ggatgatgcg
      961 gatggcgacg gtcaccttcg atcacctgtt caagcgcttt tcgggcgacg tgatcgctgt
     1021 caatgacctg agcctggaga tcgcggacgg ggagttcctc gtcctcgtcg gtccctccgg
     1081 ctgtgggaag acgaccgcgc tgcggtgcat cgccgggctc gaatcgatca gcgaggggaa
     1141 gctcctgatc ggcgaccagg tcgtcaatga cgtggcaccc aaggaccggg acatcgcgat
     1201 ggtcttccag agctacgccc tctaccccca catgagcgtc ttcgacaacc tggccttcgg
     1261 cctgaagctg cgcaagatgc cgaaggccga gatcgacacc cgggtccgcg aggcggccgg
     1321 gatcctgggc ctcgacaagt accttgaccg caagccgaag gcgctctccg gcggccagcg
     1381 gcagcgcgtc gccctgggcc gggcgatcgt ccgcgagccg aaggtcttcc tgatggacga
     1441 gccgctctcc aacctggacg ccaagctgcg ggttcagacc cgagccgaga tcgcgcggat
     1501 ccaccgacgc ctgggcacga cgatcgtgta cgtcactcat gaccaggtcg aggcgatgac
     1561 gatgggtgac cggatcgcgg tgatgaagga cggcctgctg ctgcaggttg gcaccccgga
     1621 ggagctctac acccaccccg ccaacctgtt cgtagccggc ttcatcggat ccccggcgat
     1681 gaacttcgtg accgtgacga ccgccggcgg cgagctgatg atggggcaga ccaggctgag
     1741 ccttggcggg gagccggcca gggtcgtcgc cgcacagatc ccgggcaaga gcctgacgat
     1801 cgggttccgc cccgagcatc tcaagctggc gaacgggacc accgattcgg tgatgcgctt
     1861 ccccgcggag gtcgacgtcg tcgaatacct cggcaacgaa gaactgatcc atgcgcgggc
     1921 ggaggggaac gacatcgtgg ccctcttgcc gtccgaccgg aaggtcaagg ccggcgagca
     1981 ggtcgagctg gcagtgccac tcgaccggct ctacgtcttc gacccgacgt ccgagaaagc
     2041 gctgatcaac tagcctctgc agcgaccggc gggaatcacc gggttcagcc tttaacggag
     2101 ccggccagca gccctcggac gaagtagcgc tgcagcgcga agaagacgat cagcgggatg
     2161 atcatcgaaa cgaaggcccc ggcggtcaac agctgccagt cctgccctcg agtgccggcg
     2221 aggttcacaa gcttgatggt gaggacttgc acatcctggg tgcccccgag gaaggtggtc
     2281 gccaccaggt agtcgttcca gacccacagg aattggaaga tggcgaacga tgccaggacc
     2341 ggcaccgaga ggggaatgat gatgcggcgg aagaccgcga aatgggaagc gccgtcggtg
     2401 accgccgagt cgatcaggtc tcgcgggatc tcgctcacaa aaccgaacac gaggtagatg
     2461 gcgagcggca acccgaagcc cgtgtgcgcg agccataccc cgaggtaggt tccgttcagg
     2521 ttgagccccg tgtagtcgcg caacaccggc acgagtgcga tctgcagcgg gatgacgagg
     2581 agcgccacca gcagtgcgaa gagcgccatc cgaccgcgga atcgcatcca ggcgaagcca
     2641 taggcggcga aggccgcaac cgtcagtggg atgacggtgg ctgggatggt cacggcgatg
     2701 ctgttcatga atgcgctgcc gaggtcgctc cccctctcgg ttcggagggt gccaccggtg
     2761 ttctcgtacg tcttgtcacc ccccgacagc acgacctggt aattgcgcag cgtgaagttg
     2821 aacggggcca cccactgctt ctgctgaatc tcgattcgcc ccgtgcggcg gttgccgacc
     2881 cacaggatcc gcctgccgtc cggagccgtg acgccgccgc ggaatccatc gaatgttgcc
     2941 gagaccccct cgatggtgat cggcgcatcg ccggtcacgc ccgtgggaat cgggagctcc
     3001 ttggtcgtca cgtagtcctg gtgcggcagg acggaccacc agcccgagtt gaggacgtcg
     3061 gccgctggcc gaatggagct caccaggagg ccgatggtcg gcaccagcca gatgaagcag
     3121 atgaccgcca acgttccgtt cactgcgagc tggccgagcc agcggcggcg tcttggcatg
     3181 gctacgcctg ctgctgccgg aagcgacgga ggttgtagac catcaccggg agcaccagga
     3241 tcagcagcac gatggcgatg gccgatccca ggccgaagct ccgattgctg aagaactgcg
     3301 tgtagaactc ggttgcgatc acctgggtgc cgaagttccc gttggtgagc accaccacga
     3361 cgtcgaagat cttcagcgtg aagatcacga tcgtggtcgc caccgtgatg atcgtgggca
     3421 tgatcgtcgg caccacgatg cgcatgaatg tctggatctc gttcgcaccg tccacgcgtg
     3481 ccgcgtcgag cagatcggcc gggacaccct tgatggcggc ggagaagaga accatggcga
     3541 acccggtctg gagccagacg acgaccacga tcaggaagag gttgttgagc ggctggttgt
     3601 cggcaagcca gccgatcggc gaatggccaa ggccgaccca gatcgcgttc agcaggccga
     3661 tctggggcga gctcggatcg cgaaccgcat agacaaactt ccagatgatg cccgcaccaa
     3721 cgagcgagat ggcgaccggc atgaagatga tcgacttggc gatcacctcg aaacgagaac
     3781 ggtcagcgag cgccgcgatc agcaggccca tgatgaccgt caggctcgcc ccgaacgcga
     3841 gccacagcag gttgttgcgg aaggcgagca gcgtcgcgtc gtcggtgaag acccggatgt
     3901 agttggccag ccccacgaag gccgcgccgt cctggtcgaa gaagctcgtc gcgaacgtcc
     3961 ggatcgccgg aagcaccagg aagtagacga ggaagatgac cgctggcccg atgaacacga
     4021 ccggcaggag gatctgcatc gccctggccg gcagctgctc cacgatccag ttcgagaccg
     4081 tgaagatcgc cagcaggccg ccgactcccc agatgacggc cagcagtgtg atgagcggct
     4141 tggggatcgg cgaggccttg tccgccagga tgacgtagcc cgccacaagg aagacgacgg
     4201 tcaggatcac cccgccaatg ccgagggcga ggttgcgggc ccgcgcctca ggcggcgcct
     4261 cgtcgttgaa cgcggccaat ggcggctcgg tcaatgccac tccacgactc cctcagccac
     4321 gggaacgatc gccgacgggt gcggtcaggt cggcgtcgca acaggatgcc atacgaaggg
     4381 aagaggggcg gccattttgg ccgcccctct ccggggtgat tgggacggag acgttacgac
     4441 ttggctttgt cgatgttcgc caatgcctgt tcgatagtct cgtcgccgtt gatgaacgag
     4501 gtgatctcct tccagaacgc cgtcctgacc gcaccgggca tctggtcgcc cgacgtgaat
     4561 cggatggcgg tggcgctctt cagaagggcc ccgatcttct gctcgacggc ctggccgtac
     4621 ttggacgtgt cgacgtcctt ctgcggtgca agcgcaccac cggtcgccat ccagggctcg
     4681 aggtgcttcg cgctcgtgta ccactccagc agtgcccgga cctcgggccg atcgttgaac
     4741 atgccccaga tgtcccctgc aacctgcatg tagccggcgt ggctcgcgtc aactgccggc
     4801 agcgggaaga agtcgtaatc gacgcccgcc acggctgtct tggggaagaa gctggtgatg
     4861 aagttaccct ggcgatggag ccagcacttc ggcggatcgg tgaacatcga cgcgggcgcg
     4921 tcgccgaagg ccgtgctcac gatccccttc gttccaccga agacctctcc aggcttcagc
     4981 cacatgtcag ccatggtctg ccaggcggcc ttcacctccg gagaggagaa cggcagcgtg
     5041 cccgcgaccc acttgtcgta ggtctcggga ggctggctgc cgagcatgat gtcgttgatc
     5101 aagtcggtcg ccgcccagcc ggtagccacg ccggattcga tcccgacgca ccagggggtg
     5161 tcaccatcgg cctggatctg ggccgtcagg tcggtcagct cctgccaggt cgtcggcacc
     5221 ttgtagccgg cggcatcgaa ggccttcttg ggataccaga tgaagctctt ggcgttgacc
     5281 cgctcccaga cgccggcggt gacgttgccg ctcgggcctg gcatggtggc catgtcgagc
     5341 cagctctgga cgtactgggc cttgaggtag tcagccgaca ggaacgagct caggtcgacg
     5401 gccttgcccg tctttgcgaa ggacgcaagc aagccgggct gagggaagtc cgcgacgtcg
     5461 ggagcgttgc ccgcatcgac ccggaccgag atgtcctgct cgaactgctt cgtgccttcg
     5521 tacttgacgt cgatgccagt ggcgtcctcg aagaccttga ccatggtttc gtacttggcc
     5581 tggtcctcgt cgacgaacgg cccgtacgcg gtgacgacct tgcccgtgta cttgccggca
     5641 agggcatcgt caagttcctt ggttccgctc gacgtcgcgg cggaccccga cggctgcgca
     5701 gctgtgcttc cagccgccga agccgaaggt gacgacccgc tcgagcaggc tgcgagcagg
     5761 atcgtgcccg cgaatagcag ggacgcgccc cgccaccagt gttgtctcat cgtccgattc
     5821 ctccatctgt tggatgtgcc gccaattatt gatcgcgagg gcatggttgc tccttacttc
     5881 ctcacgcgaa ttcggctgcg cggactctcg aggatgcgct catgcggacc tcatccccat
     5941 cgtcgcagca ctggattccc gaacgacgag gctcgttccc agcagtacct gcttgggaat
     6001 cgggctcctc gtttcaatgg cctccaggag cagccgcgtc atctcgcggc ccatctcctc
     6061 gatcggctga cgaatgctcg tcaacggagg ctctgaagcg gcagcaaggg ccgaatcgtc
     6121 gaacccgacc acggccacgt catctggaat gcggcgccct gctctccgca gttcgcccac
     6181 agcaccgatc gccatgagat cggacgcggc gaataccgcg tcgacgtccg ggtggcgtgc
     6241 gaggatggcc cgcatcgcca tggcgccgcc ctcctgcgtg aaatggccat tcgcctcgag
     6301 cccgtcgtcc tggacgatcc ccgcaacagc cagcgcgcgc cggtatccgg ctcggcggtc
     6361 gacgccaacc ggcatgtcga gcggtccggt gatcgtcgcc actcggcggc ggcgtccttc
     6421 cagcaggtgc tccaccgcgg tgcgggctcc gacctcgttg tcgacatcca cgtagctcac
     6481 cgtggacgag gtcggcggcc ggcccccgac caccaccggg accccacgcg tgcgcaggcg
     6541 atcgggaagc aggtcgtccc cgtgcagcga agcgagcagc gcgccatcga cgtggcctgc
     6601 ggcgacgtat gcctcggctc ggctctgggc ggacccctgc gcgatcatca gcacgagctg
     6661 gacgttgctc gccgccagct cgctgctgat gcccgccaga aggcggggaa agaacgggtc
     6721 accgaagagg ctcgtcgtct gctcgggaat gacgagcgcg atgaagccgc tgcgtcgcgt
     6781 gacgaggctc ctggccgccg gattggggac gtagcccaag cgcccaacgg cacgctccac
     6841 ggcgtgtcgg accacgggag tcaccttggg gttgccattc acgactcggg atacggtcgc
     6901 ccgcgacacg cctgccaccc gcgcgaccgc atccagggta accgcctggc gtcccgcgtg
     6961 atgctgccga tcggagatgg cagtcgcttt cctggtgaga gcgctctcac gggccatggg
     7021 agaacaatag cagggcccgt caacccccct tcggagagag cgcgatctgc ccggacgacc
     7081 ctgggggacg cgatggcgcc ggctagtcga tgtgaacctc gaccacgatc tcctggtcac
     7141 gggttcgcgg caatggcgcg agcgttccat ccacgtcggc gccgtcgacc gtgagcctgg
     7201 tcgacctgcc gaccgcgcct cgcgccttga cgacccggat ccggtaggtc gacccgcgga
     7261 agtggcgcgt gacgctgaag cccgcccatt cggcggggat ccggggatcg atccgaaggc
     7321 cgtcgtgctc ggggcgaatc cccaggatcc actggctcac cgcgacgagg ttccaggcgg
     7381 ccgtgccggt gagccaggag ttcttggcct cgccgtgcgt cgccgcgtca cggccggcga
     7441 tcatctgtgc gtatacgtac ggctcagttc ggtggatctc cgagatctcc tcgcgagccg
     7501 atgggttgat gcgcaggtag tagtcgaccg ctgcgtcggg attgccggtc atcgcctcgg
     7561 cgatcatcac ccacgggttg gtgtggcaga agatccccgc gttctccttg taaccggggg
     7621 gataggtcga gatctcccca aggttcacgt ggtagcgggt gaacgccggt tgcacgagca
     7681 tcaacccgtg gggggtggcg agccgctcgc gcaccgacgc cagcgcccgc accgcccttc
     7741 cgtctcgcag cccgatgccc gccatcacgc agatgccctg cggctcgatg aagatctggc
     7801 cctcctcgtt ctcggcggag ccgacggggg cgccgaagta gtcgtaggca cgacggaacc
     7861 aggcgccgtc ccatccgctg gagtcgaccg ccgcgtccat cgcagcggcg gcggccgcgc
     7921 agcgtcgagc ctcggccgtg tcgtcgcgtc gcgcggcgat ctcgcgcatc tcgctcgccg
     7981 ccagcacaaa aaggccggcg atgaagatcg actcggcgac cccgccgctc cggttctccg
     8041 tcgtctggaa tgactcgccc ggcgtttccg agaagcagtt gagattcagg cagtcattcc
     8101 agtcggcacg cccgatgagg ggcaggccat gcgggccgag tcgttcgagc gtgtagccga
     8161 cagcccgctg gagatgctca tacagcgccg tctcgctgcc ttgccggttg tcgtatggaa
     8221 cgggctcgtc caggaggcct gcgtcgccgg tctccttgag gtaggcagcg acggcgagaa
     8281 cgagccataa cgggtcgtcg ttgaagccgg acccgatagc gtcgttgccg cgcctggtga
     8341 ggggctgata ctggtgatac gccccgccat cgggaagctg ggtcgaggca atgtcgagga
     8401 tccgctggcg ggcccgctcg ggaatcatgt gcacaaagcc caacaggtcc tggtttgaat
     8461 cacgaaaccc catcccgcga ccgatgccgg actcgaagag cgatgcggac cgcgacacgt
     8521 tgaaggtgac catgcactgg tacgcattcc agacgttgac catccgatca aggtgctcgt
     8581 tgccggtctg cacctggagg ttcgcgagca gctcgtccca gcgcgtgcgg agagcagcca
     8641 tggcgcgatc cgcggcgtca ggctccaggt agcggtcgat gaccggccgg acgcctcgca
     8701 ggtcgacggt ggtggagcca ggcgggtcga acttcgcgtc cgcggggttc tccgcgtagc
     8761 ccaggacaaa ggtgacctcc ctcgtcgcgc cggggccaag ctcgagccgg acgtggtgag
     8821 aacccatcgg ctgccaacca tgtgcgatcg agttcgtggt ggcgccacgc tcgacgacca
     8881 ggggatcctc ccagccgcgg tacgggccca ggaacgcgtc gcgttgcgtc tcgaatcctg
     8941 caatcggctc ggagcacgcg aagtacgcga agtggttgcg acgctcccgg tattccgtcg
     9001 tgtggtagat gacgccatcc gacacctcga cctcgccgat cgagtagttg cgctgcaagt
     9061 tcgtggcgtc gtcgttggca tcccacaggc agaactccac ggcgctgaag atggagaggc
     9121 tcgccgcagt ctcgcgctcg ttggtgatgc gcgcgctcca gatctccaag gtctcggaga
     9181 gcgggacgag gtaggtcgtc tcgacgtgaa tgtcggtgaa ccgggcgccg atcacggtat
     9241 agccgaggcc gtgccgacag ctgtagtcgg tcaggtcgcg ccgcatcggc tgccagctgg
     9301 ggctccacgt cgcaccgctg tcgtcgtccc gcaggtagag atagcgaccc ccaaggtcga
     9361 gcggcgcatt gttgtaccgg tagcgcgtca gccggcgcag ccgggcgtcg cgatagaacg
     9421 agtagccgcc cgccgtattc gagatgatgc cgacgtagtc ctcgctgccg aggtagttga
     9481 tccacggcag gggcgtgtcc ggacgggtga tgacgtattc gcgtcgcgag tcatcgaaat
     9541 ggccgtaacg cacctttctc ctccgcaacg gctccgggca ttgagatcgg ccggctcggc
     9601 ggcagcactc ctcgggccgg atgagagagt accgggtcaa gcactggact tgcgtcgcag
     9661 cctgccaaac cctgcttgcg ccagccctcg ttgacggttg ctgaatcgaa cggctcggga
     9721 gcgggtaccc tctcgccatg cgtcgacccg cggatcccaa tccggcaggt tcacggccca
     9781 tggcacccct tgacggcgac ccgctctggt acaaggatgc ggtcatctac cagctgcacg
     9841 tgaaggcgtt cgccgactcg tccggcgacg gcatcggcga cttcatcggc ctcaccggca
     9901 agctcgacta catcgccagc ctgggcgtca ccaccatctg gctgatgccg ttctacccct
     9961 cgccgctgcg cgatgacggg tacgacatcg ccgactacac caacatccac ccggcgtacg
    10021 gaacccgcgc cgacttccgc gccttcgtcc gggcagcgca caagcggggc ctgcgagtga
    10081 tcaccgagct ggtcatcaac cacaccagcg accagcatcc atggttcgtg gagtcgcacg
    10141 cgtcgaggac gaatccacgc caggactggt atgtctggtc gcccgatgac aagaagttcg
    10201 ccgacgcgcg gatcatcttc aacgacaccg agacgagcaa ctgggcctgg gacaacgcgc
    10261 gccaggagta ttactggcat cgcttcttca gccatcagcc cgatctcaac ttcgacaccc
    10321 ccgccgtgct gcgcgcgatc atccgggtga tgcgcttctg gctggagatg ggggtggacg
    10381 ggctgcggct ggatgcagtc ccgtacctgg tggagcgcga gggcaccgac ggcgagaacc
    10441 tgccggagac ccacgcggtg ctgaaacggc tgcgcgcggc gatggacgag aggcaccccg
    10501 gccgcatgct gctggcagag gcgaaccagt ggccggacga ggtgcgcccc tacttcggcg
    10561 acggcgacga gtgcaacatg gcgttccact tcccgctcat gcctcgcatc tggatcgccc
    10621 tgcgcaagga ggaccgctcc ccaatcgtcg acatcatcga gcgcacgccg ccgatccccg
    10681 agaccgcgca gtgggccctc ttcctccgca accacgacga gctgacgctc gagatggtca
    10741 cccctgacga gcgcgactac atgtggaacg agtacgcagc cgaccttcgt acacgcatca
    10801 acatggggat tcgacgccgc cttgccccgc tcgtcgagaa cagccgcggc cggttggagc
    10861 tgctcaacgg cctcctgctg tcgatgccgg ggaccccggt catctactac ggcgacgaga
    10921 tcgggatggg agacaacgtg tacctcggcg atcgcaatgg cgtccgcacc ccgatgcagt
    10981 ggacgcccga tcgcaatgcc ggcttctcgg gcgccgatgc cgccgcgctc tactcgccgg
    11041 tgatcgtcga tccggtctat gggtatgggg cggtcaacgt tgaggcccag gagcggatcc
    11101 ccggctccat gttgcactgg atgcgccgca tcctgggcgt ccggcgcagc taccccgcct
    11161 tcgggcgtgg ctccctgcgg ttcctcgaac cagagaacat gcgggtgatc gcctatctgc
    11221 gcgagcacgg gggggtgacc atcctgtgcg tggctaacct gtcgcgcttc gtgcagtacg
    11281 tcgagctcga tctgcgcgag ttcgacggca tggtgccgat cgagatgatc ggccacgtcc
    11341 gcttcccgcc catcggcgag ctcccgtacc tgctgacctt cgcgccgcac gggttctact
    11401 ggttcctcct gaccgatggc gcgcacgccg gcgattgatc gccggctcca gcgcctggcg
    11461 tcagcgttca cgcctgagtg gctggcggcg caacgctggt atcgcgccaa gacccgcacc
    11521 ctggccggcg tcgagctcct cgacgccgcg gagatcgatg gctcccctgg ctggctgctc
    11581 gtgctcgagg cggcggaggc ggcaggcggc cactcccgct atctcgtgcc ggtcgtgctc
    11641 gaccgcgacc tgttttcgga gccgggggac ggcgacgggg tgtggcgcgc actcgccggg
    11701 ctgatgctcg atgggggcga gctcgtgggt actcgcggcc ggtggagctt cagtccaacc
    11761 aacgcggcct cggcgctgca gccgggcggg gctgctgcac tcgcatcact ggcggaacgc
    11821 cggctggggg tgcagcaatc gaacacatcg gtggcgctgg gcgaggccct gatgctcaag
    11881 gtgtatcggc tggtagaaac gggtgccaac cccgaggtcg agatgaatgc cttcctgacc
    11941 gaggccggct ttcacgaggc gcctgcgctc gcaggatcag ccacctacct gcttgacggc
    12001 gagccgcact cggccgccat gctccagcag ctggtgcgtt ccgcgggcga cgcctggagc
    12061 tgggtcctcg agcgcgtcac ctcggagccg gatggggcgg ctgaggctct tcgcgggatt
    12121 gcccagatcg gcaacctcac ggcggggatg cacgcggcgc tggcttctcg acccacggcc
    12181 cctggcttcc cctcgcgcgt tgccacctgg gaagaactct ccatgtggag cagctcggcc
    12241 cggcgacagc tgagcgacgc attgaaggtg ctcgacgaaa ccacccggcc ccgcctggcg
    12301 aagatcgttc ccaggatcac ggccggcctc ggcgcgatcc aggatgcagg gcaagtccgg
    12361 gtgagccgga tccacggcga ctaccacctg ggccagctcc tgagaaccga tgatgggttc
    12421 accgtgatcg acttcgaggg cgagccggcg cggacgctgg cggagcgacg ggcaccggcg
    12481 tctccgctgc gagacctggc cggcatgctg cgctcgctcg attacgcggc gcaggccgcc
    12541 gtcgccgggt actcctcacc gggcacgaac agcgagtggc tgctggcggc ccgagaggcc
    12601 ttcatcgggg gatacggggg gatcagagcg gaccaggcga ccttgctggc cgccttcgag
    12661 ctcgagaagg cgtgctacga ggtcgtgtac gaggcgaaca accggcccga ctggacctgg
    12721 ctgccgctcg gcgccctgga gcgcctcgca accggttagg cggcgagcct tgattgattc
    12781 ctcaactatc tgaccatata gttgcacagg caaccat
//
LOCUS       DATCQR010000066        11227 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_18464, whole genome shotgun sequence.
ACCESSION   DATCQR010000066 DATCQR010000000
VERSION     DATCQR010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 11227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11227
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18464"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..360)
                     /locus_tag="VIN32_02275"
     CDS             complement(<1..360)
                     /locus_tag="VIN32_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEY8921434.1"
                     /translation="MTPLSLEAPAKLNLSLRVVGRRPDGFHLLETEMVLLELADRLLL
                     LPGAPGLRVEAAPGEEIPLDQGNLAWRGLVAAIGAEPEFVCLTLEKRIPVGAGLGGGS
                     SDAAAAWRLGRRASAAPD"
     gene            complement(357..1250)
                     /gene="rsmA"
                     /locus_tag="VIN32_02280"
     CDS             complement(357..1250)
                     /gene="rsmA"
                     /locus_tag="VIN32_02280"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEY8921435.1"
                     /translation="MTGPLAPERLVPPTPGQLRRLLAAERLRPRKALSQNFLTDAAAL
                     DEIVAAAELVPGDRVVEVGPGLGVLTRRLLAAGAEVLAVELDPRLAAYLRRELSGAAG
                     FELIEADALSLHPRECFPGKDFKLVANIPYHITSPLLHAFLEGDRPPLLAVLLVQAEV
                     AERVAAAPGQMSYLSVFVQNVAETEIVGRVVAAAFEPPPEVDSAILRLRRRATPEVPV
                     GPGREPFYRLVQAGFRQRRKQLHNALGRELPVPRSALQDAFAACDIDSERRAQTLSLG
                     EWDCLNRHLAPLLGDGIAGDG"
     gene            complement(1247..2290)
                     /gene="tsaD"
                     /locus_tag="VIN32_02285"
     CDS             complement(1247..2290)
                     /gene="tsaD"
                     /locus_tag="VIN32_02285"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEY8921436.1"
                     /translation="MSGPLVLAIETSCDETGVAVVSGGRRIASNQVATQIALHAATGG
                     IVPEVAARQQLRWIVPTVEAAVSEASVDWDDLDAVAVTYGPGLIGSLLVGVNVAKALA
                     VAHDLPLVGINHIEGHIYANWLTDAPAEDPLPAEPPFPLLCLVVSGGHTQLVLMDGHG
                     QYRLLGQTVDDAAGEAFDKVGRMLGLPYPGGPAISEAAEGAAAATRFPRARTAGPYDF
                     SFSGLKTAVLREVAGYRDRGVPIPIDGLAAAFEEAVVDALATKTVAAADEHGVAAVAM
                     GGGVAANRSLRRVLGQRLDALGLPLLVPPPAWCTDNGAMIGAAAGFRFLAGDRAGPAL
                     EAIPNLSLAWSDA"
     gene            complement(2287..2805)
                     /gene="rimI"
                     /locus_tag="VIN32_02290"
     CDS             complement(2287..2805)
                     /gene="rimI"
                     /locus_tag="VIN32_02290"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008411283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEY8921437.1"
                     /translation="MSTPLVVGPMRMADVAAVLEIERLSFSSPWPAFAFEQELTANRL
                     AHYRVARLADRVVAFGGIWLMVDEAHITTFGVHPDHRRRGIGRRLLLQLAEVALELGS
                     TRMTLEVRVSNEPAQRLYQSFGFSVTGRRVAYYSDDGEDALVMTTPDLAGPAMRAMLA
                     AERARASEDRLP"
     gene            complement(2802..3437)
                     /gene="tsaB"
                     /locus_tag="VIN32_02295"
     CDS             complement(2802..3437)
                     /gene="tsaB"
                     /locus_tag="VIN32_02295"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEY8921438.1"
                     /translation="MIIAIESASTDPSLALASSDGVTLARDGWAGEGRQASELLPRLL
                     VLLARVGRDLREATALAVGVGPGSFTGLRVSMSLAKGIALALRVPVVGIPSLEAWLEA
                     EPRAAAAIARAGAQDAYLLTRGTAEPRIVAASDLRGLVGGSVSAAPSELALAFELSGT
                     LAPHGAAAAVARLAATRLAVDPRGDDLARLEPWYLRAPRGVAASDTVSGWP"
     gene            complement(3434..3910)
                     /gene="tsaE"
                     /locus_tag="VIN32_02300"
     CDS             complement(3434..3910)
                     /gene="tsaE"
                     /locus_tag="VIN32_02300"
                     /inference="COORDINATES: protein motif:HMM:NF014426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEY8921439.1"
                     /translation="MKDQGTLSTAAPEETRAIGQALGSHAQPGAMLALIGPLGAGKTE
                     LAKGVAEGLGVTSVVNSPTFVLMNEHVGRLRLFHIDAYRLDDPEEALAAGLFDDRQAA
                     GVAVVEWADRLADRLPAERLELTLVPEVDGSDRRDIRWRAVGTAHLRLAGDALTKP"
     gene            complement(3937..4977)
                     /locus_tag="VIN32_02305"
     CDS             complement(3937..4977)
                     /locus_tag="VIN32_02305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921440.1"
                     /translation="MIEASLHRIHLPRGTIVSAQVGAEIKPDEVIGLRRGAMAPVRVP
                     VTRPLHRQPGEIEELLVVKPGDRVEAEQPLALVEGGGVVLAPVAGLVLAASGRDGTLL
                     LAPLAAEESVIGHVRGRVRAVEEGSLVIEVPAARLQGVGGSGEAVHGELVVGVPNPGD
                     ELRAAAIDAGATGKILVGGSRASAETLTRARAMGVAGIVLGGVLDKELRDFEAIQRRR
                     REAGGMTGAFGILLVEGFGKVGIDPQLFAWFRTHAGRVASLFGADGLLYVYDAAPLPT
                     RRALARVGDRVIAHRRPFQGRGGVLVGMLEDLHAAQSGIPARMALVRFEDGRLAPVPL
                     ANIEATVLPEGR"
     gene            complement(4974..6641)
                     /locus_tag="VIN32_02310"
     CDS             complement(4974..6641)
                     /locus_tag="VIN32_02310"
                     /inference="COORDINATES: protein motif:HMM:NF025309.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HEY8921441.1"
                     /translation="MTTQAPAREQGRATAELSLLVDIGSAWTKAAVVGRALGRWRIVA
                     HAAQPSAWDEDELIATLAGRLAGAADARVSRRIAALLADAPRIAVHTPARPGRIALGA
                     VSSELSGQAARRAAEAAGWVVVEAVTVDDGRPVVERLSALQSAEVDAWLIAGGFDEEG
                     GEGALEVAGLVAAARGGGRSPVVWAGSAGLTERVAAFFEPGVLRTVANPRPSADRENL
                     LPLRRYLDGLIERLVERGAARQLAPIGFVRAVAELARASRLRVVGVDLGARYATWASA
                     DENGMAESRVVALGGLAAPMLTAPGGPQRIARTLPVAIDELAVADALQNLRARPGSIP
                     QTEEELAVVHGAARHLLAELAANGPQPRGIDLLIGSGRTIGSAPLPAQAMQLLLDGIR
                     PVGIVQVAIDPAGALGPLGALRDDEIVEGLGTLRDDLLTPLGAAVVCRGGRTGHLSMR
                     VTVHRVGWRSLGPIEVRPGQLEVVPLPRGQVAELEIELDPGVSLGAPRRTRRLHASVS
                     GGAVGVVLDARDAPLILPRRSDDRRAVLSAWRDTFQREVRPPAEGAA"
     gene            complement(6638..7282)
                     /locus_tag="VIN32_02315"
     CDS             complement(6638..7282)
                     /locus_tag="VIN32_02315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921442.1"
                     /translation="MNGFAGTDLAGTVATWVAAIATIAVWSYLTGSRRLFVLMQHLLA
                     GLATGYLVLLAIRDVLVPRLVVPLIERPADRVLLIPALVLVGMLIGASWLPRRLMAPV
                     AGILVGGIAAFALGGAVVGTVLPQIAAALPTTSAASPDLVGEVISLAITSLVLLAFLH
                     GAPRGRLTARAATMGRWLLVGGIGAWLGFLVVSRLSLLVDRVAFLLGDWLTVLR"
     gene            complement(7279..8127)
                     /locus_tag="VIN32_02320"
     CDS             complement(7279..8127)
                     /locus_tag="VIN32_02320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921443.1"
                     /translation="MSGETVVRLRRAAPAMLLLLTLGVASLLSGSTGGLRLASPDRSH
                     ADQLRRAIAELPSGALVLIACDADLGTYAEIRATTRAAIDDLAAHGARLAIVSFTPEG
                     RAIAAAERDRLMRSGAADPPADLGFVAGSEAGLVRSIESVVPASATGATLDAIRQRGG
                     GIGAFDMVLIVSGSDISARSWVEQVGTRLPGLSMVTIAPTFLDPELEPYLRSGQLTAL
                     LSTLRDGVAYAEAVSEGSGSSSGRIVGPLPMLVGMLVALAVLAEAAFRRLGRRARRPA
                     GRRLRS"
     gene            complement(8144..8761)
                     /locus_tag="VIN32_02325"
     CDS             complement(8144..8761)
                     /locus_tag="VIN32_02325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921444.1"
                     /translation="MRRSILIGVVLVTGLLVLLDMVIVNPSLHGAAGAVNELLVLLAA
                     AAAVGGGATLVAHHVSNLAAADGDSVASIVLLLGMAVILVAGLRPGSNGSSDPAVLWL
                     VAALLAPIAASVFALLFIFLLAAFRRGFALRVRETSLMAGAAAVVIVLLIPVGGQAGD
                     WLAAGAGWVRDVPLGGVFRGLLIGIGILVATSSARSLMGLDADDE"
     gene            complement(8767..9609)
                     /locus_tag="VIN32_02330"
     CDS             complement(8767..9609)
                     /locus_tag="VIN32_02330"
                     /inference="COORDINATES: protein motif:HMM:NF042826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6754 domain-containing protein"
                     /protein_id="HEY8921445.1"
                     /translation="MPNLGEFASHVIGLVTDAVGSSSMRLGAAPTVAILLVLLVVLSL
                     VASPGARWIASDLGRLSRVGRMMALAAESGADATISLGSAGVSRGASATDRFQTLAAL
                     PLLGHVADAAARAGVPLRVTTNDPVAVLLAEATLAGAHQRTATPERASSTSVEYVGEG
                     RSASAAYALASAQPHGVAVVAGALGEDGLLVLDGVLGDAEWSLAATASGSQLAGPLLD
                     GDGSLVGPDFFQAAGEIGPSGHARTVVLAANRLIWGAIGLLLVGSIIAWAGGPQVAAF
                     LTGR"
     gene            complement(9610..9861)
                     /locus_tag="VIN32_02335"
     CDS             complement(9610..9861)
                     /locus_tag="VIN32_02335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921446.1"
                     /translation="MEQSDSLADELERRGVAAPAAILMDAHRPLLPLLRQGVIFLGPL
                     LSPLLGARRFGVVRRALHDPAVYDRIVARLAGVRRDPPR"
     gene            complement(9903..11057)
                     /gene="alr"
                     /locus_tag="VIN32_02340"
     CDS             complement(9903..11057)
                     /gene="alr"
                     /locus_tag="VIN32_02340"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEY8921447.1"
                     /translation="MSLATRRSPHRAWIEVDHAALAANLGALRRLAGDQVSVIAVVKA
                     NAYGHGAIEVARTLLDHGAERLAVATLGEGLELRNAGIAAPILVLWALGAPEAEPAVA
                     ADLEPVVYDEAGIDLIERAAAAIGRRAAVQLKIDSGLGRQGSEPAVAVQLALRIARSR
                     HLHLTGTFSHLAVPGEDEAYSDVQRLKLAQALDAMRSAGADPGIVHLAATGGVVAGTG
                     TFGDAIRPGLGLYGMAPAWAADRELGLQPILSLKALPLRIFNLAAGEGIGYGLRFHAE
                     RATRIATLGIGYGDGWPRVHANNGWVLVRGVRRPVVGAVSMDGITVDLGDLDDVTYGD
                     EFVLIGEQMGARIDADEVAAQRRTINYEVTTALRDRLPRVHLPEVAANAG"
BASE COUNT         1774 a         4188 c         3914 g         1351 t
ORIGIN      
        1 atccggcgcg gccgacgccc gtcgaccgag gcgccacgca gccgccgcat cggacgagcc
       61 cccgccgagc cccgccccga cggggatccg cttctccagc gtgaggcaga cgaactcggg
      121 ctccgcgccg atggccgcca ccaggccacg ccaggcgagg ttgccctggt cgagggggat
      181 ctcctcgccc ggcgcggcct cgactcgcag tccgggggcg ccggggagga ggagcagccg
      241 atcggccagc tcgagcagca ccatctccgt ctcgaggagg tggaatccgt ccggtcgccg
      301 gcccaccacc cgcagtgaca ggttcagctt ggccggagcc tcgaggctga gcggagtcaa
      361 ccgtcgcccg ctatcccgtc gccaaggagc ggcgccagat gccggttgag gcagtcccac
      421 tcacccagcg agagggtctg cgcacgacgc tccgaatcga tgtcacatgc cgcgaatgca
      481 tcttgcaacg ccgagcgcgg gaccggcagc tcccgtccga gcgcgttgtg gagctgcttg
      541 cgccgttgac ggaatcccgc ctggaccagc cgatagaacg gctcgcgtcc cggccccacc
      601 gggacctcgg gggtcgcacg gcggcgcaat cgcaggatgg cggagtcgac ctccggcggc
      661 ggctcaaacg cggctgcgac gacgcggcca acgatctccg tctcggcaac gttctggacg
      721 aagaccgaca ggtagctcat ctggcccggg gcggcggcca cccgctcggc gacctcggcc
      781 tgcaccagga gcaccgccag cagcggcggc cgatccccct ccaggaaggc gtgcaacagc
      841 gggctggtga tgtggtaggg aatgttcgca accagcttga agtctttacc tgggaagcac
      901 tcgcgcgggt ggagggagag cgcatcggcc tcgatcagct cgaaccctgc ggcgccgctc
      961 aactcccgcc gcaggtacgc ggccaggcgc ggatcgagct cgaccgccag gacctctgcc
     1021 cccgcggcga gcaggcgacg ggtcagcacg ccaagcccgg gaccgacctc caccacgcgg
     1081 tcgcccggca ccagctcggc cgcggcaacg atctcgtcaa gggcagcggc atcggtcagg
     1141 aagttctggc tgagcgcctt gcgcggccgc agacgctcgg cagccagcag gcggcgcagc
     1201 tgccccggcg tgggaggcac cagccgctcg ggcgcgagtg ggccggtcac gcgtcggacc
     1261 acgccaggga taggttcggg atcgcctcga gcgcagggcc agcacgatcg ccggcgagaa
     1321 agcgaaagcc ggcggcggct ccgatcatcg ccccgttgtc ggtgcaccac gcggggggcg
     1381 ggacgagcag cgggagcccg agcgcgtcca gccgctgacc gagcaccctg cgcagcgagc
     1441 gattggcggc caccccgcca cccatcgcca ccgccgcgac gccatgctca tccgctgcgg
     1501 cgaccgtctt ggtcgccagc gcgtcgacga cggcctcctc gaaggctgcc gccaggccgt
     1561 cgatcgggat cgggacgccg cgatctcggt agccggcaac ctcgcgcagg acggcggtct
     1621 tcaggcccga gaagctgaag tcatacggcc cggcggtgcg cgcccgcggg aagcgagtgg
     1681 ccgccgccgc gccctcggcg gcctcgctga tggccgggcc gccggggtac ggcaggccga
     1741 gcatccgccc gaccttgtcg aaggcctcgc cggccgcgtc atccacagtc tggccaagga
     1801 gccgatattg gccgtgaccg tccatcagca ccagctgggt gtgcccgccg ctgaccacca
     1861 ggcagagcag cggaaacggc ggttccgcgg ggagtggatc ctcggcgggc gcatcggtca
     1921 gccagttggc gtagatgtgg ccctcgatat ggttgatccc caccaggggc aggtcgtgcg
     1981 ccaccgccag cgccttggcc acgttgacac cgaccagcag cgagccgatc aggcccgggc
     2041 cataggtcac cgccaccgcg tcgaggtcgt cccagtcgac gctggcctcc gacacggccg
     2101 cctcgacggt cggcacgatc cagcgcagct gctgacgcgc ggccacctcc ggcacgatgc
     2161 cgccggtcgc cgcatgcagc gcgatctggg tggcgacctg gttcgaggcg atgcgccggc
     2221 ccccactcac gacggcaacc ccggtctcgt cgcagctcgt ctcgatggcg agcaccagcg
     2281 gcccgctcat gggagccgat cctccgacgc ccgggcgcgc tcggctgcca gcatcgcccg
     2341 cattgccggg ccagcaagat cgggggtcgt catcacgagg gcgtcctcgc catcgtcgct
     2401 gtagtaggcg accctgcggc ctgtcacgct gaagccgaac gactggtaca gccgttgggc
     2461 tggctcgttg ctgacgcgca cctccagggt catgcgcgtg gaccccagct cgagcgccac
     2521 ctcggccagc tggagcagca ggcgtcgccc gatcccccgc cggcgatgat cgggatgcac
     2581 gccgaaggtc gtgatgtgcg cctcgtcgac catcagccag atgccgccaa aggcgaccac
     2641 ccggtctgcc aggcgcgcca cacggtagtg cgcaaggcgg ttcgcggtca gctcctgctc
     2701 gaacgcaaag gcgggccacg gcgagctgaa gctgagccgc tcgatctcga ggaccgccgc
     2761 gacgtcggcc atccgcatcg gcccgaccac cagtggtgtg ctcacggcca tccgctcacc
     2821 gtgtccgatg ctgcgacgcc ccttggcgcg cggaggtacc acggctcgag ccgcgccagg
     2881 tcgtccccgc gcggatccac ggcgagacgg gtcgccgcga gtcgtgcgac cgccgctgct
     2941 gcgccgtgcg gcgcgagcgt gccagacagc tcgaaggcga gggccagctc ggacggcgcc
     3001 gccgatacgg agccgccgac gagtcctcga aggtcagacg cggctacgat ccgcggctcg
     3061 gcggtcccac gcgtgagcag atacgcgtcc tgggcgccgg ctcgtgcgat ggctgccgcg
     3121 gcacggggct ccgcttcgag ccacgcctcg aggctgggga tgccgaccac cgggacccgc
     3181 agggcgaggg cgatcccctt ggccaggctc atcgagactc gcaggccggt gaatgagccg
     3241 ggacccaccc cgacggcgag cgccgtcgcc tcccgcaggt cgcgcccgac ccgcgcgagg
     3301 aggacaagga ggcgaggcag caactcgctg gcctggcgcc cttcacccgc ccagccgtcc
     3361 ctggcgagcg tgacgccgtc gctcgaggcc aacgcgagcg atgggtcagt ggaggcgctc
     3421 tcgatggcga tgatcacggc ttggtcaggg catcgccggc gaggcgcagg tgggcagtcc
     3481 caaccgcgcg ccagcggatg tcgcgacggt cgctcccgtc cacctcgggg accagggtca
     3541 gctccagtcg ctccgctggc aggcggtccg cgaggcgatc ggcccactcc accacggcca
     3601 cccctgccgc ctggcgatcg tcgaagaggc cggcggctaa cgcctcctcg ggatcgtcga
     3661 ggcggtaggc gtcgatatgg aagagccgca gccggccaac gtgctcgttc atgagcacga
     3721 acgtcggact attgacgacg ctcgtgaccc ccagcccctc ggcaaccccc ttggccagct
     3781 ccgtcttgcc ggctccgagc gggccgatca gcgccagcat ggcccccggc tgagcgtggg
     3841 agcccagcgc ctggccgatc gccctcgtct cctcgggcgc agccgtgctg agcgtcccct
     3901 gatccttcat ggcccgaagg atacgggagc ggccgctcag cggccttcgg gcagcacggt
     3961 ggcctcgatg ttggccagcg ggaccggggc gagccggccg tcctcgaagc gaaccagcgc
     4021 catgcgggcc ggaatccccg attgggcggc gtgcaggtcc tccagcatcc ccaccaggac
     4081 gccaccgcgc ccctggaacg ggcgccggtg ggcgatcacg cgatccccga ccctggccag
     4141 ggcgcgacgc gttggcagcg gagccgcgtc atagacgtag agcagcccat cggctccgaa
     4201 caggctggca acccgcccgg catgcgtgcg gaaccacgcg aagagctggg gatcgatccc
     4261 gaccttcccg aagccctcga ccagcaggat cccgaacgcg ccggtcatgc cgccagcctc
     4321 gcgccggcgg cgctggatcg cctcgaagtc gcgcagctcc ttgtccagca cgccgccgag
     4381 cacgatccct gccactccca tcgcgcgggc tcgggtcagc gtctcggccg aggctcgcga
     4441 tccaccgacc aggatcttgc cagtggcgcc ggcatcgatc gccgcggccc gcagctcatc
     4501 gcccggattc ggcacgccga ccaccagctc cccgtgcacg gcctcgccac ttccgccgac
     4561 cccctgcagt cgagcagcgg ggacctcgat cacgagcgat ccttcctcga cggcgcgcac
     4621 gcgaccgcga acgtgcccga tcaccgactc ctcggcggcg agcggagcga gcaggagcgt
     4681 cccgtcgcgt cccgaagccg ccagcaccag gccggcgacc ggggcgagca ccaccccgcc
     4741 cccctcgacc agggccagcg gctgttcggc ctcgacgcgg tcgcccggct tcaccaccag
     4801 gagctcctcg atctcgcctg gctgccggtg gagcgggcgc gtgaccggca ccctgaccgg
     4861 cgccatcgcc ccgcgccgca ggccgatcac ctcgtcgggc ttgatctcgg cgccgacctg
     4921 cgcgctgacg atggttccgc gaggcaggtg gatgcgatgc agcgacgcct cgatcatgcc
     4981 gccccctcgg ccggtggccg aacctcgcgt tggaaggtgt cgcgccaggc gctgagaacc
     5041 gcccgtcggt cgtcgctgcg gcgcgggagg atgagtggag cgtcgcgggc atcgaggacc
     5101 acgccgaccg cgccgccgct cacggacgcg tgcaaccgac gcgtgcgacg cggagcgccg
     5161 aggctgaccc cgggatcgag ctcgatctcc agctcggcca cctgcccgcg tggcaggggc
     5221 accacctcga gctggccggg acgcacctcg atcgggccga gcgatcgcca gccgacgcga
     5281 tggaccgtga cccgcatcga gagatgaccg gtccgcccac cgcggcagac gaccgcggcg
     5341 ccgagtggcg tcagcaggtc gtcccgcaac gtaccgagcc cttcgacgat ctcgtcgtcg
     5401 cgcagcgctc cgagcgggcc caacgccccg gccgggtcga tcgcgacctg cacgatgccg
     5461 accggccgga tgccgtcgag cagcagctgc atcgcctggg ccggcagcgg cgcgcttccg
     5521 atcgtccgtc ctgagccgat cagcaggtcg attcccctgg gctgcggacc gttcgccgcc
     5581 agctccgcca gcaggtgccg cgccgcgcca tgcaccaccg ccagctcctc ctcggtctgg
     5641 gggatcgatc cggggcgggc gcgcaggttc tgcagtgcgt ccgccacggc cagctcgtcg
     5701 atcgccacgg gcagggtgcg cgcgatccgt tgtgggccgc cgggcgccgt cagcatcggt
     5761 gcggcgagac cacctaacgc gacgacccgg ctctcggcca tgccgttctc gtccgcgctg
     5821 gcccaggtcg catagcgggc accgaggtcg accccgacga cgcgcaggcg ggaggcgcga
     5881 gcaagctcgg ccaccgcccg tacgaacccg atcggagcca gctgccgtgc cgcaccgcgc
     5941 tcgaccagcc gctcgatcag cccgtcgaga tagcgccgca gcggcagcag gttctcgcgg
     6001 tccgcggagg gacggggatt cgcgaccgtc cgcaggactc ccggttcgaa gaaggccgcc
     6061 acccgctcgg tgaggcccgc tgagcctgcc cacaccactg gcgatcgacc gccgccacgg
     6121 gccgccgcca ccaggccggc cacttcgagc gccccctcgc ccccctcctc gtcgaagccg
     6181 ccggcgatga gccaggcgtc gacctcggcc gactgcagcg cggagaggcg ctccaccacc
     6241 ggccggccgt catcgacggt aaccgcctcg acgaccaccc agccggcagc ctccgcggca
     6301 cggcgcgccg cctgcccgga aagctccgag gagacggccc ccagggcaat gcggccgggc
     6361 cgtgcaggtg tgtgcaccgc gatccggggc gcgtcggcga gaagtgccgc gatccgcctc
     6421 gagacgcggg cgtcggcggc gcctgccagc cgtcctgcca gggttgcgat cagctcgtcc
     6481 tcgtcccagg cgctcggctg tgctgcgtgc gccacgatcc tccatcggcc gagagcacga
     6541 ccgaccacgg ccgccttcgt ccaggcggag ccgatgtcga ccagcagcga cagctcggcg
     6601 gttgcgcgtc cttgctctcg ggcgggggcc tgggtcgtca tcggagaacg gtaagccaat
     6661 cgccgaggag gaacgcgact cggtcgacaa gcagcgagag tcgactcacg acgaggaacc
     6721 cgagccatgc cccgattccc ccgaccagga gccagcggcc catggtcgcg gcgcgggccg
     6781 tcagcctccc tcgcggcgcc ccgtgcagga aggccagcag cacgagcgag gtgatggcca
     6841 gggagatcac ctcgcccacc aggtccggcg acgcggcgct cgtggtcggg agggcggcgg
     6901 cgatctgcgg gaggacggtg ccgaccactg cgcctcccaa cgcgaaggcg gcgattcccc
     6961 cgaccaggat cccggcgact ggtgccatca ggcgtcgcgg gagccatgag gcgccgatca
     7021 gcattcccac cagcacaagt gcagggatga gcagcacccg gtcagcagga cgctcgatca
     7081 ggggcacgac gagtcgtggc acgaggacgt cgcggattgc cagcagcacc aggtacccgg
     7141 tggcgagacc cgcaagcagg tgctgcatca ggacgaagag ccgccgtgag ccggtcaggt
     7201 acgaccacac ggcgatggtg gcgatggcgg ccacccaggt cgccaccgtc ccggccaggt
     7261 cggtgccggc gaagccgttc atgaccgcag gcgccggccg gcagggcgcc gggcacggcg
     7321 accaagccgt cggaaggcag cctcggccag caccgccagg gcgacgagca tgccgaccag
     7381 catcggcagc ggcccgacga tccgccccga gctggagcct gacccctcgg agactgcctc
     7441 ggcgtaggcc actccatcgc gaagcgtgga caggagcgcg gtgagctggc ccgagcgcag
     7501 gtagggctcg agctcagggt cgaggaaggt gggtgcgatc gtcaccatcg acagcccggg
     7561 gaggcgtgtc cccacctgct caacccagct gcgcgccgag atgtcgctgc ccgagacgat
     7621 caggaccatg tcaaaggcgc cgatgccacc gccccgctga cggatcgcgt ctaacgtcgc
     7681 gcccgtcgcc gaagcgggga cgaccgattc gatgctgcgc accaaccccg cctccgaccc
     7741 cgccacgaac ccgaggtcag caggtgggtc cgccgctccg ctgcgcatga ggcggtcacg
     7801 ctccgccgca gcgatggcgc gaccctccgg cgtgaagctg acgatcgcca accgagcacc
     7861 atgagccgcg agatcgtcga tcgcggcgcg cgtcgtcgcc ctgatctccg cataggttcc
     7921 caggtcggcg tcgcaggcga tcaggaccag ggcgccgctc gggagctcgg cgatcgccct
     7981 ccgcagctga tcggcgtgcg atcggtccgg gctggccagc cgcaatccgc cggtgctgcc
     8041 cgagaggagc gatgccaccc cgagcgtcag cagcaggagc atcgccgggg cggcacgacg
     8101 cagccggacg acggtctcac cgctcacaag agtcggtgat gcgctactcg tcatcggcgt
     8161 cgaggcccat cagggagcgg gccgagctgg tggcgacgag gatcccgatc ccgatcagca
     8221 gcccgcggaa gacgccaccc agcggcacgt cgcgaaccca gccggcaccc gcggccagcc
     8281 agtcgccggc ctggccgcca acagggatga gcaggacgat gacgaccgca gcggcaccgg
     8341 ccatcagcga cgtctcgcgg acccggagcg cgaaccctcg ccggaaggcg gcgagcagga
     8401 agatgaagag gagggcgaag accgatgccg cgatcggtgc gaggagcgcc gccaccaacc
     8461 acagcacggc cgggtcggag ctcccgttcg atccgggtcg caggccggcc accaggatca
     8521 cagccatccc gagcagcagg acgatcgagg ccaccgaatc cccatccgcc gcagcgaggt
     8581 tgctgacgtg gtgggccacc agcgtggcgc caccaccaac cgcggccgca gccgcaagca
     8641 ggacgagcag ctcgttcacc gcccccgccg cgccgtgcag ggaggggttc acgatcacca
     8701 tgtccagcag caccagcagg cccgtcacca ggaccacgcc gatgaggatc gagcgacgca
     8761 tcgggctcag cgtcccgtca ggaaggcagc gacctgcggg ccgccggccc acgcgatgat
     8821 cgaaccgacc agcaggaggc cgatggcgcc ccagatcagg cggttcgccg ccagaacgac
     8881 cgttcgggca tggccgctgg ggccgatctc gcctgccgcc tggaagaagt ccggaccgac
     8941 caggctcccg tcgccgtcga gcaggggccc tgccagctgg gagccgctcg ccgtggcggc
     9001 gagactccac tcggcatcac ccagcacgcc gtccagcacg agcaggccat cctcgccgag
     9061 cgcaccggca acgaccgcca cgccgtgcgg ctgggccgaa gcgagggcgt aggcagcgga
     9121 ggccgatcga ccctcgccga cgtactccac cgacgtcgag gaggcgcgtt ccggcgtggc
     9181 ggtccgctga tgcgcacccg ccagcgttgc ctctgcgagc agcacggcga ccgggtcgtt
     9241 ggtggtcacc cgcaggggga cgccggcccg cgccgcggca tctgcgacgt ggccgagcag
     9301 gggcagcgcc gcgagggtct gaaaccgatc ggtggcagag gcgccgcggc tcacgcccgc
     9361 gctgcccaac gagatcgtcg cgtcggcccc ggactcggcg gcgagcgcca tcatgcgacc
     9421 aacccgggag aggcgaccaa ggtccgatgc gatccagcgc gcgccggggc tcgcaaccag
     9481 cgacaggacc acgagcagga ccagcaggat cgcgaccgtc ggggcggcgc cgagccgcat
     9541 cgaggatgag ccgacggcat cggtcacgag gccgatcacg tgcgaggcga actcgccgag
     9601 gttgggcatc tagcgcgggg gatctcggcg cacaccggcc agcctggcga cgatgcggtc
     9661 gtagacggca gggtcatgga gtgcccggcg caccacgccg aaccggcgag cgccgagcag
     9721 cgggctcagg agcgggccga ggaagatgac tccctggcga aggagcggca gcaggggtcg
     9781 gtgcgcatcc atcaggatcg ccgctggcgc ggcgactcca cgccgttcga gctcatcggc
     9841 aagggagtcc gactgctcca cgctgcattc tccctgcgag acaccctgct cgctcgacga
     9901 cctcacccgg cgttcgcggc gacctccggc agatgcacgc gcgggaggcg gtcacggagg
     9961 gcggtcgtca cttcgtagtt gatggtgcgt cgctgggcgg caacctcatc ggcgtcgatg
    10021 cgggcaccca tctgctcgcc gatcaggacg aactcgtcgc cgtaggttac atcatcaaga
    10081 tcgcccagat cgacggtaat gccgtccatg ctcaccgccc caacgacggg gcgacgaaca
    10141 ccccgcacca ggacccagcc gttgttggcg tgaacgcgtg gccacccatc gccgtacccg
    10201 atccccagcg tggcgatgcg cgtcgcacgc tcggcatgga agcgcagccc gtagccgatc
    10261 ccctctcctg ccgccagatt gaagatccgc agcggtagcg ccttgagcga gaggatcggc
    10321 tgcagaccca gctcacggtc ggcggcccag gcaggagcca tgccgtaaag tcccagcccg
    10381 ggccgaatgg catcgccaaa ggtgccggtg ccggccacca cgccccccgt ggccgccagg
    10441 tgaacgatcc cgggatcggc tccggccgat cgcatggcgt cgagggcctg ggccagcttg
    10501 agccgctgga catcgctgta cgcctcatcc tcgcccggaa cggcgaggtg gctgaaggtc
    10561 cccgtcaggt gcagatgccg gctgcgggca atgcgcaacg ccagctggac cgcaactgcg
    10621 ggctcgctgc cctggcgccc caacccggag tcgatcttga gctggacggc cgcccgacga
    10681 ccgattgccg ctgccgctcg ttcgatcagg tcgatcccgg cctcgtcgta gaccaccggc
    10741 tcgagatcgg cggcgacggc cggctcggcc tccggcgctc cgagcgccca caggacgagg
    10801 atcggggccg cgattccggc attccgcaac tcgagccctt cgcccagtgt ggcgacggcg
    10861 agccgctcgg caccgtggtc caggagggtc ctcgccacct cgattgcccc gtggccgtag
    10921 gcgttggcct tgaccacggc gatcacgctc acctggtctc cggccaggcg gcgcagcgcc
    10981 cccaggtttg ccgccagggc ggcatgatcg acctcgatcc aggcgcggtg ggggctgcgc
    11041 ctggtggcca ggctcattgg ccctgcccgt cacgaaggtg ctggatggcc gtcggcagca
    11101 ggttggcgac gtcccgcgcc accacgccgg cctgaccgat ccgctcctcg gccaacaggc
    11161 cggccgcgcc atgcaccgcc acgccacagc cggccgcgtc caacggctcg agcccggcgg
    11221 ccaggaa
//
LOCUS       DATCQR010000067         4195 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_111588, whole genome shotgun sequence.
ACCESSION   DATCQR010000067 DATCQR010000000
VERSION     DATCQR010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4195
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111588"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="VIN32_02345"
     CDS             <1..250
                     /locus_tag="VIN32_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020673997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY8921448.1"
                     /translation="PRSSVALYRVGQASALLAGRSFVLPDDVRTVAHAVLAHRLLLEV
                     DRELRWATVTGVVQEVIDSVPVPMADEEPIAGDRPSTA"
     gene            253..1485
                     /locus_tag="VIN32_02350"
     CDS             253..1485
                     /locus_tag="VIN32_02350"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEY8921449.1"
                     /translation="MGDWIALASAMAVLAVVADAPGVLLVAVLTLGYGSLRTVWTRYG
                     MRRVEYSRRLGTGRAVAGDSVPLDITIWNRKRLPLPWVTAEDRLGERLTVRERPEMHV
                     DEYQQGTHLLRNDWALQWYERVVRHFHLDDVRRGSYRFGPVQLSVRDFLGRLASEERI
                     ELSDTLVVTPAMAAVRHAEHEMSPLGERRARHSLTVDPALFAGVRPFQAGDSLRLVHW
                     RATARLGSAVSRRLEPARGRTMMVVVDVQTVDGPVLTWDEDAFESLTVVAASLARRLL
                     ADGASLGMAAANFAGSAQKFAWLAPRASMAQLPRAGELLARIGPVPSAPLSALLAWLT
                     KRMPAETALILVTARAPGPHVAALRRMKLMGFGVELVTVGPDAAVNAQTARMARMRAM
                     TATVEPSWEEPDAVVLAG"
     gene            1463..2794
                     /locus_tag="VIN32_02355"
     CDS             1463..2794
                     /locus_tag="VIN32_02355"
                     /inference="COORDINATES: protein motif:HMM:NF024948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HEY8921450.1"
                     /translation="MRSSWPVRVLLPAAQALAEGSWLAVLYAALQASAKEIPYLGPLE
                     LGALVMAGTAWRRRRRGRSPSVEGLGLALLVLLSGVFGWLLAPAVRVALSEGDLIRAL
                     SLHVPGWLAALAFWRGQAHGFPVDDTVTEHRLARWAVPGLAIPWLLGYAFADGQLQDD
                     FAAAALIGTLFFIGSAFTALGLKRLEALRLSTGRDWRGDRSWLFMIIGLALVLTVGSV
                     PIAALLGIPARSLLGAIVGPLQTLILLLVLLTAPVFLAAAIVAGWLGSTLPAEVLKLP
                     TFNAPRGEPGSDLPFIILGVIVASIFIFEFLALLVLLWMVYGGRRRGDPLDEPFEERA
                     IVPPPDDEDDAPPVSAASRRRIPVSDEVTAAYLAALDALAIDGRWPRRANESPAAHLA
                     RIRAAGFGSGAFGRLAAAYQLVRYGGRSLRDRERSRSRSRLDGVRALLTRS"
     gene            2986..3339
                     /locus_tag="VIN32_02360"
     CDS             2986..3339
                     /locus_tag="VIN32_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009139943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEY8921451.1"
                     /translation="MSSLRDPQRPRYFISIAAELAQVHPRTLRIYEEEGLLCPQRRNN
                     LRLYSEADIKRVRIIRFLTRRQGVNLAGVKVILQLEADGKIRVYDLFDGDDVERYVEE
                     DAPEPVSASTEGTRS"
     gene            3342..>4195
                     /locus_tag="VIN32_02365"
     CDS             3342..>4195
                     /locus_tag="VIN32_02365"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HEY8921452.1"
                     /translation="MDDKNTEPEVEQIQVLPLVALRDTVIFPEMIVPLQVGRDRSVKA
                     LDRAVRTSQPVALITQRSNETEEVNSVDELYSIGTLAKIAQVIRLQDGTIRAIVQGQK
                     RIRLLDLVQTEPHLEARIELIDDEQEKSLEVEALMASVQGQIEQYVSSGASVPPEVAV
                     AARNISDGGLLADMVAYSPDMTTEARQQLLETIDVAERLRTVGQFLAKQIEVLELKGR
                     IQSEVKSEMDKTQREYILREQLKAIQKELGEDDPAVAEAGELKTKVEESAMPADVKEK
                     ALKEVDRL"
BASE COUNT          636 a         1402 c         1464 g          693 t
ORIGIN      
        1 gccacgcagc agtgtcgcgc tgtaccgcgt gggacaggca tcggcgctgc tcgccgggcg
       61 cagtttcgtg ctgcccgatg acgtccggac ggtggcgcac gcggtgctcg cgcatcggct
      121 cctgctcgag gtcgaccggg agctgcgttg ggctaccgtg acgggcgtcg tccaggaggt
      181 catcgacagc gtgccggttc caatggccga tgaagagccg atcgcgggag accggccctc
      241 gacggcgtag gcgtgggcga ttggatcgct ctcgcatccg cgatggccgt gctggccgtg
      301 gtggcggacg cgccgggggt cctgctggtg gctgtgctga cgctcggtta cggatcgctc
      361 aggaccgttt ggactcgata cgggatgcgg cgggtcgagt acagccggcg gctggggacc
      421 ggtcgtgcgg tcgccggcga cagcgttcca ttggacatca cgatctggaa ccgcaagcgc
      481 ctgccgcttc catgggtcac cgccgaggat cggctcggcg aacggctgac cgtccgcgag
      541 cgaccggaga tgcacgtcga cgagtatcag cagggaacgc acctgcttcg caacgactgg
      601 gcgctgcaat ggtatgagcg cgtggtacgg cacttccatc tcgacgacgt acggcgaggc
      661 agctaccggt tcgggccggt ccagttgagc gtgcgcgact tcctcggccg cctggcgagt
      721 gaggagcgga tcgagctatc cgacaccctc gtggtcactc ccgcgatggc agcggtccgg
      781 cacgccgagc acgagatgtc gccgctcggg gagcggcgcg cgcgccactc cctgactgtc
      841 gaccccgcgc tgttcgccgg tgtccggcca ttccaggcgg gcgactccct gcgcctggtc
      901 cactggcgcg ccaccgcacg gctcggcagc gccgtcagtc gccggctgga gcctgcccgc
      961 gggcggacga tgatggttgt ggtcgacgtg cagaccgtcg atgggcccgt gctcacctgg
     1021 gacgaggacg ccttcgagag cctgacggtg gtcgcagcct cgctggcgcg acggctgctt
     1081 gccgatggcg cgtccctggg catggccgcc gcgaattttg cgggctcggc ccagaagttc
     1141 gcgtggcttg cgccccgtgc ctcgatggcg cagctgccac gagccggcga gctgcttgcg
     1201 cgcatcgggc ccgtgccgtc agcgccgtta tcggcgttgc tggcgtggct gaccaagcgc
     1261 atgcccgcgg agacggcgct gatcctggtg accgcgcgcg ctcccggtcc gcacgtggct
     1321 gcgctgcgcc gcatgaagct gatgggattt ggcgtcgagc tggtgaccgt cgggccggat
     1381 gcggccgtca acgcccagac ggcgcggatg gcacggatgc gggcaatgac cgctaccgtc
     1441 gagccgagct gggaggagcc cgatgcggtc gtcctggccg gttagggtcc tgttgccagc
     1501 tgcccaggcg ctggccgagg gcagctggct ggccgtgctc tacgccgcgc tgcaggcgag
     1561 cgcgaaggag atcccgtacc tgggtccgct ggagctcggg gcgctggtca tggccggcac
     1621 cgcctggcgt cgccgtcgac gagggagatc gccttcggtc gagggtctgg gactggctct
     1681 gttggtgctc ctttcaggcg tgttcggctg gctgctggct cccgctgttc gcgtggcgct
     1741 gagcgagggc gatctcatcc gcgcgctcag cctgcatgtt cccggctggc ttgctgcgct
     1801 ggccttctgg cggggacagg cgcacggctt ccccgtggac gacaccgtca ccgagcaccg
     1861 gctcgcgcga tgggccgtcc ccggactcgc cattccgtgg ctactcggct atgcattcgc
     1921 agacggacag ctgcaggacg acttcgccgc cgccgcgctg atcggcaccc tctttttcat
     1981 cggctcggcg ttcacggcgc tgggcttgaa gcggctggag gcgctgcgcc tgtcgaccgg
     2041 cagggactgg aggggcgacc ggtcgtggct gttcatgatc atcggcctgg ccctggtgct
     2101 gaccgtcggc tcggtgccga tcgccgccct cctcggcatc ccggcgcgga gcctgctggg
     2161 cgccatcgtt ggccccctgc agaccctcat cctcctgctg gtcctgctga cggcgccggt
     2221 cttcctcgcc gccgcaatcg ttgccggctg gcttggctca acgctgcccg cggaggtcct
     2281 caagctgccg acattcaacg ccccacgcgg cgagccgggc tcggacctgc cgttcatcat
     2341 cctcggcgtc atcgtcgcct cgatcttcat attcgaattc ctcgccctgc tcgtcctgct
     2401 gtggatggtg tacgggggcc ggcgaagggg agatcctctc gacgagccat tcgaggagcg
     2461 cgcaatcgtg ccgcccccgg acgatgagga cgacgctccc ccagtgtcgg ccgcgagtcg
     2521 gcgccgaatc ccggtctcgg atgaggtcac cgccgcgtac ctggcagccc tcgacgcgct
     2581 ggcgatcgat gggcgatggc cgcgccgcgc gaacgaatcg cctgccgctc acctggctcg
     2641 gatccgggcg gcgggcttcg gctccggagc attcgggcgc ctggcagccg cataccagct
     2701 ggtgcgctac gggggccgct cactccgcga tcgggagcgg tcgagaagcc gctctcgcct
     2761 cgacggcgtc cgcgccctgc tcaccagatc ctgagcccat cggccgagca cgaaacggag
     2821 ctgcgcagcc tgctgatcgg caagtagggc ctgcgcctcg ggcagcacca tccttgacaa
     2881 tggcattggt agggattacg gtaccgccac gttccaatat cacgcaacgt gaagtggtcc
     2941 gaacatggcc ttcatcaccc gataccacct ctatggcgag ctgctttgag ctcactgcgc
     3001 gatcctcagc gtccgcggta cttcatcagc atcgcggccg aactggccca ggtgcatccg
     3061 cggacgctgc ggatctacga ggaggagggc ctcctctgcc cacaacggcg caacaacctc
     3121 cggctctact cggaggccga tatcaaacgg gtgcgtatca tccgttttct cacccgacgc
     3181 cagggggtca acctggcggg cgtcaaggtc atccttcagc tcgaggcgga cggcaagatc
     3241 cgcgtctacg acctgttcga tggagacgat gtcgagcgat acgtcgagga ggacgcgccg
     3301 gagccggtga gcgcctcgac tgaagggaca cgaagctgaa catggatgac aagaacaccg
     3361 agcccgaggt cgagcagatc caggtgctcc cactcgtcgc cctgcgcgac acagtgatct
     3421 ttccggagat gatcgtcccg ctgcaggtcg gccgcgaccg cagcgtcaag gcgctcgacc
     3481 gcgcggtccg cacctcgcag ccggtcgcgc tcatcacgca acggagcaat gagaccgagg
     3541 aggtcaactc ggtcgacgag ctctactcca tcggcacttt ggccaagatc gcccaggtga
     3601 tccgcctgca ggacgggacc attcgggcca tcgtccaggg acagaagcgc atccgcctgc
     3661 tggacctggt gcagaccgag ccccacctcg aggctcgcat tgagctgatc gacgacgagc
     3721 aggagaagag cctcgaggtc gaggccctga tggcatcggt ccaggggcag atcgagcagt
     3781 acgtgagctc cggagcgtcc gtgccgccgg aggtcgcggt cgccgcgcgc aacatctccg
     3841 acggcgggct gctggccgac atggtcgcct acagcccaga catgaccacg gaggcacggc
     3901 agcagctgct cgagacgatc gacgtcgccg agcgtctgcg caccgtcggg cagttcctgg
     3961 ccaagcagat cgaggtgctg gagctgaagg gtcgcatcca gagcgaggtc aagtccgaga
     4021 tggacaagac ccagcgcgag tacatcctgc gcgagcagct caaggcgatc cagaaggagc
     4081 tcggcgagga cgatcctgcc gtcgccgagg ctggcgagct gaagaccaag gtcgaggagt
     4141 cggccatgcc tgccgacgtc aaggagaagg cgctcaagga ggtggaccgg ctcaa
//
LOCUS       DATCQR010000068         6223 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_219781, whole genome shotgun sequence.
ACCESSION   DATCQR010000068 DATCQR010000000
VERSION     DATCQR010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6223
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219781"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1074
                     /locus_tag="VIN32_02370"
     CDS             <1..1074
                     /locus_tag="VIN32_02370"
                     /inference="COORDINATES: protein motif:HMM:NF020048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEY8921453.1"
                     /translation="VLGSFLPQYYAATERPPREVVLPFAPSEADAMAAFLADRRGSKV
                     ALTVPERGDKRRLVALATQNAVEALSRERAEWLADAGKRDEALEQLATALGLGRAPER
                     IECYDMSNIQGTSAVGSMVVFVNGRPEPREYRRFRIRSGDTPDDFRMMAEVIRRRFSR
                     ASRLRAETGALSLAAVGADPAFEALGDEAADAEARDESRPRTAARDAGWALPDLVIVD
                     GGKGQLSAAMGVMRDLDLGDVPVAGLAKRFEELYLPGRPEPIILPRNSQGLYLVQRLR
                     DEAHRFAITYHRNVRSRRALASVFDEVEGIGPGRKKALLKRFGSVRRIREASVDELAE
                     TPGIPREVAERLKGHLAREGMLA"
     gene            1190..2524
                     /gene="secD"
                     /locus_tag="VIN32_02375"
     CDS             1190..2524
                     /gene="secD"
                     /locus_tag="VIN32_02375"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEY8921454.1"
                     /translation="MTWRRTAPWVILVTLLLAIFIDLPRTTLGLGWLPSNVFGHELKT
                     VLGLDLQGGIRVTLAVTPQSGQTITDEQVETARNIIERRVGGLGVSEPQVRTEVSGNQ
                     RRIVVEIPGLSSGDEDRVRSLVGSTGQLQFIDPKGQTLTVDQDIRPLIADGSVAELFD
                     GSQIDPGSVNPGTNNGQIGVDFTLSSDGSAKWCQFTTANVNKPGPIALDGRVITTPTI
                     NQAICAGQTIITVGSPGQANEIERTNLYNTLRFGALPVSLSEQGVETVAPTLGADFLV
                     QALVAGGIGLTLVLLFMIAYYRLPGVLAAGALIFYSLVTYAVFRLIGVTLTLAGVAAF
                     ILSIGMAVDANILIFERMKEEIRSGKTLGPAIEAGFNRAWSSILDSNVSSLLVASWLF
                     WQGTTVVRGFALVLIIGVLLSMFSAVTVTRTVLRMVVRTDWGRRLELYHVER"
     gene            2529..3638
                     /gene="secF"
                     /locus_tag="VIN32_02380"
     CDS             2529..3638
                     /gene="secF"
                     /locus_tag="VIN32_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEY8921455.1"
                     /translation="MYDIVGKRNWYFAFSALITIPGLIFILLGGLKPSVDYTGGTVWQ
                     VRYADVPSAAEVHAALVELRHPEAVVRSVGGGFLEIRTRPIDLLPQSTQVPTPSPLPS
                     GVTPEPSPSPSPSPSPTPEPSGSAPASASASASASESPSPSPTPAPVIPGTPFDTLEK
                     QLIARFGAGEPRLVTTVGPTVGSELIRSSIILIVIGELFILGYLWVRFGFRYGTAAIV
                     ALLHDVIVVVGIFAMLGFFFGLEFDALFVTALLTIIGFSVHDTIVVFDRIRENRVRHA
                     GEPFGAIVNHSILQTLGRSINTSLTVVVTLTALLLLGPATIRTFALALLIGVISGTYS
                     SIFNASQLLAVWYEWDAKRKASALAAARKGRPTAR"
     gene            3698..5482
                     /gene="recJ"
                     /locus_tag="VIN32_02385"
     CDS             3698..5482
                     /gene="recJ"
                     /locus_tag="VIN32_02385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEY8921456.1"
                     /translation="MIVPRLEWLLPEPFPEPPPFAGFGRPLATLLARRGFRDDEQLGR
                     FLHAGEASLHDISLMADADVALDRIERAIAAGEQMAIWGDYDADGMTAVVVWVLAFRA
                     LGIEAQRHVPSRLAEGYGLSRSGLERLAAAGVTLLITCDCGVTNSVEVEEARALGMEV
                     IVTDHHLPSGPLPRAIAVVDPHRPDCAYPDPDLTGAGLAFKLASALLARRGQAAADLA
                     GVAAIGTIADMAPMTGESRAIVRLGLDELAVTTRPGLRALLARSAEVPDQPTARDLGF
                     GVAPRINAAGRIADAELAMSLLLEDDPATAERIAEELEAVHVERRAITTATIEAALAL
                     AIAMPGEGPLALRGDGWAPGVLGLVANRLVESLGRPVAVAAPVDDELRGSVRAPADFH
                     AALALEACAPLLLKRGGHPAAGGFSLRTADWADFSTAFAALPRPFPPDPGRAAERATA
                     LTVDLVLPAMHLGWPLAEEIACLAPFGPGHVEPLLAVTGLHVAEARRVGVNGDHVSMR
                     MRRGAEVIDAIAFGTPPDRPLPEPGVALDLVGTLERDLFQGLPRLRIRVADYAEAAVS
                     PLAARRIAATTVAVPVGEAAPLEASPAR"
     gene            5479..>6223
                     /gene="pyrF"
                     /locus_tag="VIN32_02390"
     CDS             5479..>6223
                     /gene="pyrF"
                     /locus_tag="VIN32_02390"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR02127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEY8921457.1"
                     /translation="MNDRPAPFDRLRARVARLGAPLCLGIDPHPDALPDELPRDVSGI
                     ERFARGLIEAAAEYAVAVKVNVAFFEAFGSAGWAALERVVADVPPDHFLLLDAKRGDI
                     GSTAERYAASLFGLLGADAVTLSPYLGEDSVEPFLAYPDRLVYLVARTTNPSAGTLQD
                     LPVGGTPLHEHVATWAARRWTDGRVGLVVGATAPAELARLRRLVPALGFLVPGVGAQD
                     GDLAAALENCHGTAAPGLVSISRGIAGASQ"
BASE COUNT          854 a         2195 c         2200 g          974 t
ORIGIN      
        1 gtgctcggct ccttcctgcc gcagtactac gcggcgaccg agcggccgcc gcgcgaggtg
       61 gtgctgccct tcgcaccatc ggaggccgat gcgatggccg ccttcctcgc cgaccggcgc
      121 ggctcgaagg tggcgctgac cgtgcccgag cgcggggaca agcgccggct ggtggcgctc
      181 gccacgcaga atgcggtcga ggcgctgtcg cgcgagcggg cggagtggct cgcggatgcc
      241 ggcaagcgcg acgaggcact cgagcagctc gccaccgcgc tggggctggg ccgcgctccc
      301 gagcggatcg agtgctacga catgagcaac atccagggca ccagcgcggt cggcagcatg
      361 gtggtcttcg tcaacggccg cccggagccg cgggagtatc ggcgcttccg gatccgatca
      421 ggcgacactc ccgacgactt ccggatgatg gccgaggtga tccgtcgccg cttctcgcgg
      481 gcgtcgcgcc tgcgcgccga gacaggggcc ctttcgctgg cggcggtggg ggcggacccg
      541 gccttcgagg cgctcggcga cgaggcagcg gatgccgagg cccgggatga gagccgacct
      601 cgcactgcgg cgcgcgacgc cggctgggca ttgccggacc tcgtgatcgt cgacgggggc
      661 aaggggcagc tctccgccgc aatgggcgtc atgcgggacc ttgacctggg cgacgtgccg
      721 gtggcggggc tggccaagcg cttcgaggag ctgtacctgc ccggccgccc ggagccgatc
      781 atcctgccgc gcaacagcca gggcctgtac ctggtgcagc gcctccggga cgaggcccat
      841 cggttcgcca tcacctacca ccggaacgtg cgcagccgcc gcgcgctggc cagcgtgttc
      901 gacgaggtgg aggggatcgg gcccgggcgc aagaaagcgc tcctcaagcg ctttggcagc
      961 gtgcgccgaa tccgggaggc atcggtcgac gagctcgcgg agacgcccgg gattccgcgc
     1021 gaggtggccg agcggctgaa ggggcacctg gcccgcgaag gcatgctcgc ctgagattgc
     1081 gaggccatct gagcgtgcct atactggcgc ggcccgccgc aagtgtgcgg cgcgtttggt
     1141 gtctgcggca gccgcgacga ccggagcgag aggttcacct ggctcacata tgacctggcg
     1201 ccgcaccgcc ccgtgggtga tcctcgtcac cctgctgctg gcgatcttca tcgacctccc
     1261 ccgaacgaca ctcggcctgg gttggctgcc ctcgaacgtc ttcggccacg agctgaagac
     1321 ggtgctgggt ctcgacctgc agggcgggat cagggtcacc ctggcggtga ctccccagtc
     1381 gggccagacg atcaccgacg agcaggtcga gaccgcgcgc aacatcatcg agcgacgcgt
     1441 cggtggcctc ggcgtgtcgg agccccaggt ccgtaccgag gtgagcggca accagcgccg
     1501 gatcgtggtc gagatccccg gcctctcgag cggcgacgag gaccgagtgc gcagcctggt
     1561 cggctcgacc gggcagctgc agttcatcga tccgaagggc cagacgctga cggtcgacca
     1621 ggacatccgc ccgcttatcg ccgatgggtc ggtggcggaa ctcttcgatg gcagccagat
     1681 cgacccgggg agcgtgaacc cgggcacgaa caacggccag atcggggtcg acttcacgct
     1741 gagcagcgat gggtcggcca agtggtgtca attcacgacc gcgaacgtca acaagcctgg
     1801 gccgatcgcc ctcgacggac gcgtcatcac gacgccgacc atcaaccagg cgatctgcgc
     1861 tggccagacc atcatcaccg ttgggtctcc cgggcaggcg aacgagatcg agcgcacgaa
     1921 cctctacaac accctgcgct tcggggccct ccccgtctcg ctcagcgaac agggggtcga
     1981 gaccgttgcg ccgacgctcg gggcggactt cctggtccag gccctcgtcg cgggcgggat
     2041 cggccttacc ctcgtgcttc tcttcatgat cgcctactac cggctccccg gagtcctggc
     2101 ggccggagcg ctgatcttct actcgctcgt cacctacgcg gtcttccggc tgatcggcgt
     2161 cacgttgacg ctggccgggg tcgccgcctt catcctgtcc atcggcatgg cggtcgacgc
     2221 caacatcctc atcttcgagc gcatgaagga ggagatccgg tcaggcaaga cgctcggccc
     2281 ggcgatcgag gccgggttca accgggcatg gtccagcatc ctggactcga acgtcagctc
     2341 gctgctggtc gcctcctggc tcttctggca gggcaccacc gtggtgcgcg gcttcgcact
     2401 ggtgctgatc atcggtgtgc tgctctccat gttcagcgcg gtgacggtga cgcgaacggt
     2461 gctgcggatg gtggtgcgca ccgattgggg ccggcgcctc gagctctacc acgtggagcg
     2521 ctgacgacat gtatgacatc gtgggcaagc gcaactggta cttcgccttc agcgcgctga
     2581 tcacgattcc ggggctgatc ttcatcctgc tgggtgggct gaagccatcg gtcgactaca
     2641 ccggcgggac ggtctggcag gttcgctacg ccgatgtgcc gagcgcggcc gaggtccacg
     2701 cggcgctcgt tgagctccgg cacccggagg ccgtcgtgcg cagcgtggga ggcggcttcc
     2761 tcgagattcg gaccagaccg atcgacctcc tgccgcaatc gacacaagtg ccgacaccgt
     2821 cgcccctccc ctccggggtc acgccggagc catcgcccag cccgtcgccc agtcccagcc
     2881 cgacacctga gccgagcggc tcggcacccg catccgcctc ggcttcagcg agcgccagcg
     2941 aaagcccatc gccgagcccg acccccgcgc cggtgatccc gggcacaccg ttcgacaccc
     3001 tggagaagca gctgatcgcg cgcttcggcg cgggcgagcc gcgcctcgtc acgaccgtcg
     3061 ggccgaccgt cggttcggag ctgatccgga gctccatcat cctgatcgtc atcggcgagc
     3121 tgttcatcct cggctacctg tgggtccgct tcgggttccg ctacggaacc gcggcgatcg
     3181 tggcgctgtt gcacgatgtg atcgtggtgg tcggcatctt cgcgatgctc ggcttcttct
     3241 tcggcctcga gttcgacgcg ctcttcgtca ccgccctgct gaccatcatc ggcttctcgg
     3301 tccacgacac catcgtggtc ttcgaccgga tcagggagaa ccgggtgcgc cacgcaggcg
     3361 agccattcgg cgcgatcgtc aaccattcga tcctgcagac gctgggtcgc tcgatcaaca
     3421 ccagcctgac ggtggtggta acgcttaccg ccctgctgct gctcggtccg gccacgatcc
     3481 gcacgttcgc cctcgccctg ctgatcggcg tcatcagcgg cacctactct tccatcttca
     3541 acgccagcca gctcctcgcc gtctggtacg agtgggacgc gaagcgcaag gcgagcgccc
     3601 tggcggctgc tcgcaagggt cgtccgacag ctcgctgacg acggcagata agcagccgat
     3661 acctccctga taagcgcgcc gcggctacca tgaccgggtg atcgtcccgc gcctcgaatg
     3721 gctgctgccg gagccgtttc cggagcctcc gccgttcgcc ggcttcgggc gtccgctggc
     3781 gacgctgctg gcccggcgcg ggttccgcga cgacgagcag cttggccgct tcctgcatgc
     3841 cggcgaggcg tcgctgcacg acatctcgct gatggccgat gccgacgtgg cgctcgaccg
     3901 aatcgagcgc gcgatcgcgg cgggggagca gatggcgatc tggggtgact acgacgccga
     3961 tgggatgacc gcggtcgtgg tctgggtgct cgccttccgg gcgctgggga tcgaggcgca
     4021 gcgccacgtg ccatcgcgcc tcgccgaggg ctacggactc tcgcgcagcg gcctcgagcg
     4081 gctggcggcg gccggggtca cgctgctgat cacctgcgac tgcggcgtga cgaattcggt
     4141 ggaggtggag gaggcacgcg ccctgggcat ggaggtgatc gtcacggatc accatctgcc
     4201 atccggcccg ctgccgcgcg cgatcgcggt ggtggacccg caccggccgg actgcgccta
     4261 tcccgacccg gacctgaccg gtgccgggct ggcattcaag ctggcatccg ccctgctcgc
     4321 gcggcgcggc caggcggccg cggacctggc gggcgtggct gccatcggca cgatcgccga
     4381 catggctccg atgacgggcg agagccgtgc catcgtacgg ctgggcctcg acgagctggc
     4441 cgtgacgacg cggcccggcc ttcgcgccct gctcgcgcgg tcggcagagg tgcccgacca
     4501 gcccacggcg agggacctgg gattcggggt tgcgccgcgc atcaacgccg ccggccggat
     4561 cgccgacgcg gagctcgcca tgtcgctgct gctcgaggac gacccggcca cggcggaacg
     4621 catcgccgag gagctcgagg ccgtgcacgt cgagcgccgc gcgatcacga ccgcgaccat
     4681 cgaggcggcg ctggcgctgg ctatcgcgat gcccggcgag gggccgctcg ccctgcgcgg
     4741 agatggctgg gcgccgggcg tgctcgggct cgtggccaac cgattggtcg agtcactcgg
     4801 gcggccggtg gcggtggccg cgccggtgga cgacgagctg cgaggatcgg tccgtgcccc
     4861 ggctgacttt cacgccgcgc ttgcactcga ggcgtgcgca ccgctgctgt tgaagcgcgg
     4921 cggccacccg gctgcgggcg gcttcagcct ccgcaccgcc gactgggccg atttctccac
     4981 cgccttcgcg gccctgccgc gcccattccc gcccgatccc gggcgagccg ccgagcgtgc
     5041 cactgcgctg acggtcgacc tggtgctgcc cgcgatgcat ctcggctggc cacttgccga
     5101 ggagatcgcc tgcctcgcgc cgttcggacc gggtcacgtt gaaccgctgc tggcggtgac
     5161 cggactgcat gtcgccgagg cgcgtcgcgt cggcgtcaac ggggatcacg tctcgatgcg
     5221 gatgcgccgg ggagccgagg tcatcgacgc gatcgccttt gggacgccgc ccgatcggcc
     5281 cctgccggag ccgggggtcg cgctcgacct ggtcgggacc ctggagcggg acctgttcca
     5341 gggactgccc cgcctgcgaa tccgggtggc cgactacgcc gaggcggcgg tcagcccgct
     5401 cgcagctcgg cggatcgccg caaccacagt ggcggtcccg gttggggagg cggctccgct
     5461 cgaggcgtcg cccgcccgat gaacgatcga ccggctccgt tcgaccggct ccgggcgcga
     5521 gtcgcgcggc ttggtgcgcc gctctgcctg ggaatcgacc ctcatccgga cgcgctgccc
     5581 gacgagttgc cgcgtgacgt gtcggggatc gaacgcttcg cccggggcct gatcgaggcg
     5641 gccgccgagt acgcggtggc ggtgaaggtc aacgtggcct tcttcgaggc cttcggctcc
     5701 gctggctggg ccgccctgga acgggtcgtc gccgacgtcc cgcccgacca tttcctgctg
     5761 ctcgacgcca agcggggcga catcggctcg accgcggaac gctatgcggc ttccctcttc
     5821 ggcctgctcg gcgcggacgc cgtgaccctg tcgccctacc tgggggagga cagcgttgag
     5881 ccgttcctcg cctatcccga ccgactcgtc tacctcgtcg cgcgcaccac caacccaagc
     5941 gcggggacgc tgcaggacct gccggtgggt ggcactccgc tgcacgagca cgttgcaacg
     6001 tgggcggcca ggagatggac cgatggacgg gtcgggctgg tcgtcggggc caccgcccca
     6061 gccgagctgg cgcgcctgcg gcgcctggtg ccggcactcg gcttcctggt ccccggcgtc
     6121 ggtgcccagg acggcgacct ggccgcggcc ttggagaatt gccatggcac ggcggccccc
     6181 gggctggtca gcatctcgcg cggcatcgcg ggggcgtcgc agg
//
LOCUS       DATCQR010000069         3017 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_112014, whole genome shotgun sequence.
ACCESSION   DATCQR010000069 DATCQR010000000
VERSION     DATCQR010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3017
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112014"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            90..575
                     /locus_tag="VIN32_02395"
     CDS             90..575
                     /locus_tag="VIN32_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005266063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY8921458.1"
                     /translation="MSKTQIDAPAGVPFVDITREFDAPLELVRRAWTDPDLVKQWLGP
                     RKYEMVIERWEPRAGGAYRYIHRDDAGNEFGFHGVFHSNDTDNMVQTFEFEGMPGHVS
                     LDKLELSDIGDGRTRAHIHSVFQSLEDRDGMVGAGMGDGMSEGFERMDELLATMPMPV
                     G"
     gene            912..2138
                     /locus_tag="VIN32_02400"
     CDS             912..2138
                     /locus_tag="VIN32_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013132919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4032 domain-containing protein"
                     /protein_id="HEY8921459.1"
                     /translation="MRFQMLPRGSWPDFLDLPWSEPLAGWESPRIVEVARGISRHVVR
                     FVDYDGQLYALKELPEPLAEREFRLLRELDAASVPVVEVVGVVSGRNDLDAVLITRHL
                     EFSLPYRTLFTQRGISDLHHRLLDAVAELLVRLHLAGFFWGDCSLSNTLFRRDAGSLA
                     AYLVDAETGELHPSLSDGQRTYDLSIAEENFAGELLDVMAMLAEQDEAPSSSIDDPLE
                     VAAELRPRYESLWSELTRDEVIAPNETYRIDERLRRLNELGFDVEEVELLTVDGGYRL
                     RLPTRVVEAGHHRRRLLSLTGLDVGENQARRLLNDIARYRAELEAAEGRSLPDSVVGY
                     RWRAEVFEPAVAAIPPELHAKREPAELFHELLNHRWYRSEAAGHDVGMADAVASFSQQ
                     VLPEVPDERATIGSSS"
     gene            complement(2268..>3017)
                     /locus_tag="VIN32_02405"
     CDS             complement(2268..>3017)
                     /locus_tag="VIN32_02405"
                     /inference="COORDINATES: protein motif:HMM:NF044379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEY8921460.1"
                     /translation="RGELQLAVLVEQMRREGFELQVSRPEVILTTVEGVTMEPYERIT
                     VDIPPDFIGEVQAALAGRKAKLEQMSTDADGRVRLEFVLPVRGLIGYRGQLLTETRGT
                     ALLHQIAEGNGPWAGEVTHRTSGVLVSDRAGVSNSFALFNLQERSEPFIGTGVEVYEG
                     MIVGENSRPGDLDVNPTKEKKLTNMRTHAHDEALRLTPPRAMTLESAIEFIAADELVE
                     ITPSSIRLRKRILSQHGRRRAAGREYELGGT"
BASE COUNT          485 a         1045 c          998 g          489 t
ORIGIN      
        1 gagtaccagc gcttctggga cgagcgctac ggccagctcg acgagctcct ctcccgactt
       61 cagcagacgc aacgaaagga acagcaacca tgagcaagac ccagatcgac gcaccggcag
      121 gcgtcccatt cgtcgacatc acccgcgaat tcgacgctcc ccttgagctc gtccgtcgtg
      181 catggaccga tccggacctc gtcaagcagt ggctcggccc gcgcaagtac gagatggtga
      241 tcgagcgctg ggagccgcgc gcgggcggag cctatcggta catccaccgc gacgacgccg
      301 gaaacgagtt cggcttccac ggtgtcttcc acagcaacga cacggacaac atggtccaga
      361 ccttcgagtt cgaggggatg ccgggtcatg tctcgctcga caagctggag ctctcggaca
      421 tcggtgacgg gcggacgcgc gcccacatcc attccgtctt ccagtcgctc gaggatcgcg
      481 acggcatggt gggggccggg atgggcgatg gcatgagcga gggcttcgag cggatggacg
      541 agctcctggc aacgatgccg atgcccgtgg ggtgacgcac gtcgagaagg cgggcgcgct
      601 gagcgaccgc cttctccggt cgttgcgtat tcgcccagcg catcagatcg cgccggaagc
      661 tcccttcccg tgtcgcctca ttcccccggc gaatggcacg gcacgggaag ccggctatcg
      721 gtgcgtttct atgccacagg cgcataggct cgccttcgga ccagcctcca cataagcctg
      781 gcttatggtc ttgcacggga gctcccgcat cggagccacc tgataagcca ggctgatctc
      841 acttcaccga tgaagcccga tcggtgcacg agcagaagcc acgcttatga gacacccgta
      901 tcctgagacc gatgcgcttc cagatgctgc cgcgcggatc gtggccagac ttcctggacc
      961 tgccctggtc ggagcccctg gcgggctggg agtcgccgcg catcgtggag gtggcgcgcg
     1021 ggatcagccg ccacgtcgtc cgcttcgtcg attacgacgg ccagctgtac gccctcaagg
     1081 agctgcccga gccgctcgcc gagcgtgaat ttcggctcct gcgagagctg gatgccgcgt
     1141 cggtcccggt ggtcgaggtg gtcggcgtgg tcagcggccg caacgacctg gacgcggtgc
     1201 tgatcacgcg tcacctcgag ttcagcctcc cctatcggac cctcttcacc cagcgcggca
     1261 tctccgacct gcaccaccgc ctcctcgatg ccgtggcaga gctgctggta cgcctccatc
     1321 tggccggctt cttctggggc gactgctcgc tctccaatac cctcttccgc agggacgccg
     1381 gatcgctggc ggcatacctg gtcgatgccg agacgggcga gctgcaccca tcactctccg
     1441 atgggcagcg cacctacgac ctgtccatcg ccgaggagaa cttcgccggc gagctgctcg
     1501 acgtgatggc gatgctggcc gagcaggacg aggcgcccag ctcgagcatc gacgacccgc
     1561 ttgaggtcgc cgctgagctg cgcccccgct acgagagcct gtggagcgag ctgacccggg
     1621 acgaggtgat cgccccgaac gagacgtatc gcatcgatga acgcctgcgc cgcctcaacg
     1681 agctgggctt cgacgtcgag gaggtcgagc tgctcaccgt ggacggtggg tatcgcctgc
     1741 gactgccgac ccgggtcgtg gaggcggggc accatcggcg ccggctgctg agcctgacgg
     1801 gactcgacgt cggcgagaac caggcgcgac ggctgctgaa cgacatcgcc cggtaccgtg
     1861 cggagctgga agcggcggag gggcgcagcc tccccgactc ggtcgtcggc taccgctggc
     1921 gtgccgaggt cttcgagccg gcggtggcgg caatccctcc cgagctgcac gccaagcgcg
     1981 agccggcgga gctcttccac gagctgctca accaccgctg gtaccgatcc gaggccgcgg
     2041 gccacgacgt cggcatggcc gatgcggtgg ccagcttcag ccagcaggtg ctgccagagg
     2101 tgcccgacga gcgggccacc atcggctcgt cgagctgacg acgcccgacc gatcacccag
     2161 ggttatcgag atgcaccgga aggctgccgg gcgagccgcc tggtcactcc tggttatcgc
     2221 gcgacaccgg agcaagccca tcggtgcggg accataaccc ctggttatca ggtgccgccc
     2281 agctcgtatt cacggcccgc cgctcggcgt cggccgtgct gcgacaggat gcgcttgcgg
     2341 aggcggatcg aggagggggt gatctccacc agctcatcgg ccgcgatgaa ctcgatcgct
     2401 gactccaggg tcatggcccg gggtggggtc aggcgcagcg cctcgtcgtg cgcgtgggtc
     2461 cgcatgttgg tgagcttctt ctccttagtg gggttgacgt ccaggtcgcc ggggcggctg
     2521 ttctcgccca cgatcatccc ctcgtagacc tccacgccgg tgccgatgaa cggctcgctc
     2581 cgctcctgca ggttgaagag cgcaaacgag ttcgagaccc ccgcccggtc gctgacgagg
     2641 acccccgagg tccgatgcgt gacctctccc gcccacgggc cgttcccctc ggcgatctgg
     2701 tgcaggagcg ccgtgccacg cgtctcggtc agcagctgcc cgcggtagcc gatcagcccg
     2761 cggacgggga gcacgaactc gaggcgcacg cgcccgtcgg catcggtcga catctgctcc
     2821 agcttcgcct tgcggccggc cagggccgcc tgcacctcac cgatgaagtc cggcgggatg
     2881 tcgacggtga tgcgctcgta gggctccatg gtgacgcctt ccacggtcgt caggatcacc
     2941 tcgggacggc tcacctgcag ctcgaacccc tcgcgccgca tctgctcgac cagcaccgcc
     3001 agctgcagct cgccgcg
//
LOCUS       DATCQR010000070         2371 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_19127, whole genome shotgun sequence.
ACCESSION   DATCQR010000070 DATCQR010000000
VERSION     DATCQR010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2371
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19127"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..843
                     /locus_tag="VIN32_02410"
     CDS             <1..843
                     /locus_tag="VIN32_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014627714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HEY8921461.1"
                     /translation="HDPFNPDHVEISTHRLLAKMPTIAAWAYKKSIGQPYVYPRNDLP
                     YAANFLNMMFSVPAEPYEVDPVVAEALDLLLVLHADHEQNCSTSTVRMVGSSHANLFA
                     SISAGLDALWGPLHGGANQAAIEMLEQIAADGGDVARYVARAKDKKDGFRLMGFGHPV
                     YHNFDPRAKIIKAAADRVLHALGRDDPLFEIAQRLAEVALHDEYFIERKLYPNVDFYS
                     GLIYRAIGFPNSMFTAMFAIGRMPGWIAHWREMIADPATRVGRPRQVYVGAGARDYLS
                     LEQR"
     gene            854..1633
                     /locus_tag="VIN32_02415"
     CDS             854..1633
                     /locus_tag="VIN32_02415"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY8921462.1"
                     /translation="MRLTVLGRSPARPNPGEACAGYLVEGGGSRILMDVGPGVVAQLL
                     RRNTPDELDAVVISHMHTDHCLDLITLRYSYPWIDVAKHRLTVIVPPGSTGQLAEMAL
                     GAGYANFFDKSFIFVEHDGKRPIEIGNLRLEPEETQHYVRTWGFRVSATGTDEDPDRI
                     LTYSADSGPCDALPRLADGADLFLCEAALRSLKEDDPEPQSRGHLLPSEAAATARDAG
                     AKKLLLTHIPIADGVDWALSEARAAYDGSVEVAEPLRSYEV"
     gene            complement(1621..2160)
                     /locus_tag="VIN32_02420"
     CDS             complement(1621..2160)
                     /locus_tag="VIN32_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921463.1"
                     /translation="MPDEPQPQLPEDGPTRLVEGNDGELVERLSGGTATLLLVWLIGV
                     GAGSALLAAWLLAGLVLQLRLGTEHLLGAPGLYVSLYGATGPTVLWMTGRAQGRSLGW
                     FVVTAAKIGLVMAAILVGGLLLVTVVMGAALGPGALRVTIAVFGFTLAVSVLWALATW
                     SADRYIASARVVSEGDQTS"
     gene            complement(2164..>2371)
                     /locus_tag="VIN32_02425"
     CDS             complement(2164..>2371)
                     /locus_tag="VIN32_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015450761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEY8921464.1"
                     /translation="AEATRLFTLAESLGAVESLIELPALMTHLSVAGSPLEVPAGLVR
                     LSVGIEAVEDLLADLEAALQAASE"
BASE COUNT          398 a          864 c          771 g          338 t
ORIGIN      
        1 cacgacccgt tcaaccctga ccacgtcgag atcagcaccc atcggcttct cgccaagatg
       61 ccgacgatcg cggcctgggc atacaagaag agcatcgggc agccgtacgt gtacccacgc
      121 aacgacctcc cctacgccgc caacttcctg aacatgatgt tcagcgtccc cgccgagccg
      181 tacgaggtcg acccggtggt cgccgaggcg ctcgacctgc tgctggtgct gcacgcggac
      241 cacgagcaga actgctccac ctccaccgtc cgaatggtcg ggtccagcca cgccaacctg
      301 ttcgcgtcga tcagcgccgg gttggacgcg ctgtgggggc cgctgcacgg tggcgccaac
      361 caggcggcga tcgagatgct cgagcagatc gcggcggacg gcggcgacgt cgcccggtac
      421 gtggccaggg ccaaggacaa gaaggatggc ttccggctga tgggcttcgg gcaccccgtg
      481 taccacaact tcgacccgcg ggccaagatc atcaaggccg ccgccgatcg cgtgctgcat
      541 gccctgggcc gcgacgatcc gctcttcgag atcgcccagc gcctcgcgga ggtggcgctg
      601 cacgacgagt acttcatcga gcgcaagctg tacccgaacg tcgacttcta ctccggcctg
      661 atctatcggg ccatcggctt cccgaactcg atgttcaccg ccatgttcgc gatcggtcgg
      721 atgcccggct ggatcgcgca ctggcgcgag atgatcgcgg acccggcgac cagggtcggt
      781 cgcccccgcc aggtgtacgt cggcgcgggc gcgcgcgact acttgtccct cgagcagcgc
      841 tgaggccctg tagatgcggt tgacagtgct ggggcgcagc cccgcgcggc ctaatccggg
      901 cgaggcatgc gccggctacc tggtcgaggg tggcgggtcg cgcatcctga tggacgtggg
      961 ccccggtgtt gtggcccagc tcctccgccg caacacgccg gacgagctgg acgcggtcgt
     1021 gatcagccac atgcacaccg accactgcct cgacctgatc acgcttcgct acagctatcc
     1081 gtggatcgac gtcgcaaagc atcggttgac cgtgatcgtg ccgcccggtt caaccggcca
     1141 gctggccgag atggcgcttg gcgcgggcta cgccaacttc ttcgacaaga gcttcatctt
     1201 cgtggagcac gacgggaagc ggccgatcga gatcggcaac ctgcgcctgg agccggagga
     1261 gacgcagcac tacgtccgca cctggggctt ccgtgtctcc gccaccggga ccgatgagga
     1321 tcctgaccgc atcctgacct actcagctga ctcgggtccc tgcgacgcgc tgccgcgcct
     1381 ggccgatggc gccgacctgt tcctgtgcga ggccgcgttg cgatccctca aggaggacga
     1441 tccggagcca cagagccgcg gccatctgct cccctcggag gcggccgcca ccgcgcgcga
     1501 cgcaggagcc aagaagctcc tcctgaccca catcccgatc gccgatggcg tggactgggc
     1561 gctgtccgag gcgcgggctg cctacgacgg ctcggtcgag gtcgcggagc ccctgcgcag
     1621 ctacgaggtc tgatcgccct cggagacgac ccgggcgctg gcgatatagc gatccgccga
     1681 ccaggtggcg agtgcccata gcaccgagac cgccagcgtg aagccaaaga cggcgatcgt
     1741 cacgcgcagc gccccgggcc ccagcgccgc ccccatcacg accgtcacca gcagcaggcc
     1801 gccgaccagg atcgcggcca tcaccagccc gatcttggca gcggtcacca cgaaccagcc
     1861 cagggatcgg ccctgcgcgc gaccggtcat ccacaggacg gtcgggccgg tcgcgccgta
     1921 cagcgaaacg tagagtcccg gtgcgccgag gaggtgctcc gtgcccagac gcagctgcag
     1981 aaccaatccg gccagcagcc aggccgccag cagcgcgctc cccgccccga cgccgatcag
     2041 ccacaccagc agtagcgtcg cggtcccgcc agagaggcgc tcgaccagct cgccatcgtt
     2101 gccctcgacc agcctggtcg gcccatcctc ggggagctgt ggctggggct cgtcgggcat
     2161 cttctattcg gacgctgcct gcaacgccgc ctccaggtcg gcgagcaggt cctcgaccgc
     2221 ctcgatcccc accgacaggc gcacgagccc ggccggcacc tcgagtggcg agccggcgac
     2281 cgagagatgg gtcatcagcg ccggcagctc gatcagcgac tcgaccgcgc caagcgactc
     2341 ggcaagggtg aagaggcggg ttgcctccgc c
//
LOCUS       DATCQR010000071         5843 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_3866, whole genome shotgun sequence.
ACCESSION   DATCQR010000071 DATCQR010000000
VERSION     DATCQR010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5843
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3866"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..538
                     /locus_tag="VIN32_02430"
     CDS             <1..538
                     /locus_tag="VIN32_02430"
                     /inference="COORDINATES: protein motif:HMM:NF020133.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921465.1"
                     /translation="LGRRASAAPDAATPADLATLCRLGADVPFFAAGAAAALVTGIGE
                     VVQPLGAAAPDHAILVHPGFGLSTAAVFDELRSADLAAARPNGNDLLAPALRLRPELG
                     DLMRQVSEAGAEPHLTGSGSTIFALTDDPERAAAIAAGLRRTGVRVTLTRVRGKPAEI
                     LELGEAPQDAGATTPSMG"
     gene            549..971
                     /locus_tag="VIN32_02435"
     CDS             549..971
                     /locus_tag="VIN32_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007491276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family detoxifying hydrolase"
                     /protein_id="HEY8921466.1"
                     /translation="MLDKGVMTVRDEVLTDAAPAAIGPYSQAMRVGGFVFTAGQVGAE
                     PSDGALADGVAAQADRALRNLAAVLAAAGSSFDRVVKTTIFLVDMGDFQAVNEVYARH
                     LSPPFPARSTIAVKALPKGALVEIEMIALASDEEPARS"
     gene            968..2380
                     /gene="glmU"
                     /locus_tag="VIN32_02440"
     CDS             968..2380
                     /gene="glmU"
                     /locus_tag="VIN32_02440"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR01173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEY8921467.1"
                     /translation="MTGRAAVVLAAGQGTRMRSHLPKVLHPLAGRPMLGHVLAALDKA
                     GIEQLVVVVGHDGEQVEAVVGGTATTVRQEPQRGTADAVRIGLERLDPGTQQVVVAMG
                     DAPLVPAVLFEELLSAQAAGDACIALLASRPHDPSGYGRVVRDAGGSVEAIVEENDAD
                     ARVLELGEVNAGTYCFDAGWLRANIGAVPASRSGEHYLTDLVAIAVSSGRSVVVVNAP
                     RAELAMGINDRSGLATAERVLRSEIAERHMRDGVTIVDPSSTFIDADVQIGEDARIEP
                     WTILAGATTIGPKAVIGPQAQVLDSKIGARTTIWSSVVESSVVAEDVQIGPYSHLRPG
                     CEIGPGCRIGNFAELKMTRVGAGSQIHHLSYLGDADLGEAVNIGAGTITANFDGKVKH
                     RTNIGDGAFIGVDTMLRAPVSVGPGARTGAGAVVTRDVPAGETVVGMPARRIGTRRRR
                     TEVAADRNPDPGGGKPRSDA"
     gene            2373..3776
                     /locus_tag="VIN32_02445"
     CDS             2373..3776
                     /locus_tag="VIN32_02445"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEY8921468.1"
                     /translation="MPEPGVGGSATVNPVLALLIVGVLVVVSGFFAASELSLITVKRY
                     RITQLASEGNRSAKAAERLLEDPSRFLATIQVALTFLGFLASAVGAVSFSGPVADFIR
                     LIPWAPIKEAAPTIAFLLTTMIIALVTIVIGELAPKTLALSFTERFALAVARPIAFMG
                     RLLSPFVWLVSLISGFLVRALGGKNRPQAGYLSTEELKMLVETGSEQGGIEEDEKEMI
                     HGVIELADTQVHEVMVPRIGIRAVDVNDPMEEVLDLIVRAGHSRVPVYEENLDNIIGI
                     LYAKDLLPYLKGNGGGAPDIDIRKLARPPVYVPETKAVDELLHEMQVTKRHIAIVVDE
                     YGGTAGLITLEDVVEEIVGEIEDEYDREESLVEPLEEEGKVAFRVDGRVSMDDLRDLF
                     DLSDTEEEDEDEYDTVGGFIVHRVGRIPLPGAVVPFRDVTLTVEAAEPRRIAKVIATR
                     PREPESETGEVAAADAD"
     gene            complement(3773..4822)
                     /locus_tag="VIN32_02450"
     CDS             complement(3773..4822)
                     /locus_tag="VIN32_02450"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEY8921469.1"
                     /translation="MSQFNDLLVEIEDEMRSVIGERDGSARPLYEMLAYHLGLDQMEG
                     PRGKRLRPLLGLLAFKSFGGEYRKALPAAAAVELGHNFSLVHDDIEDSDRERRHRPTL
                     WALWGVPLAINAGDALFALSRLALYRLMKEEEYEPQKLLDVMRVYDETCLALCEGQYL
                     DISFERRTDVTVDEYLEMISKKTAALIGASIETSAMMASDDEEVIGAYHRLGRDLGMA
                     FQIADDLKGSFWASADSGKAAAGDIRKRKKTYPVVWAMRNAAAADVARLIAIYQPVIR
                     ATDGSGPPDGDVPMSEELTEEVLEILERSGARAATEQEARRYRDLALVEARSLPVPPE
                     RLEDLRTLVESMISA"
     gene            4862..5647
                     /gene="recO"
                     /locus_tag="VIN32_02455"
     CDS             4862..5647
                     /gene="recO"
                     /locus_tag="VIN32_02455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEY8921470.1"
                     /translation="MSRARLYKTEGIVLRSMDLGEADRVLTVLTPRLGKLRVIAKGIR
                     RPRSRLGGGLEPFSDVHLVLAVGRTFDVVTQASLEDPHLGLRDDLYSTAAAWYVVELA
                     DRFCEGSAESHQAFELLAQALAALDAAPEAVSREVVARWFELHLLEAMGFRPELGQCL
                     DCGVEIEPDGNAYSPVAGGVIGPECTHTALGTRQISAAALKVMRYLQRSPLTEVLRLR
                     LSSPTQREVEHLLHASVSAVLERELRSRDFLDEVALRGIPAPA"
     gene            5704..>5843
                     /locus_tag="VIN32_02460"
     CDS             5704..>5843
                     /locus_tag="VIN32_02460"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014684354.1"
                     /note="Catalyzes a two-step reaction, first charging a
                     glycine molecule by linking its carboxyl group to the
                     alpha-phosphate of ATP, followed by transfer of the
                     aminoacyl-adenylate to its tRNA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HEY8921471.1"
                     /translation="MPAPNMELIVSLAKRRGFVFPSSEIYGGMGGFWDYGPLGVELKN
                     NV"
BASE COUNT          893 a         1978 c         2046 g          926 t
ORIGIN      
        1 cctcggtcga cgggcgtcgg ccgcgccgga tgcggcaacc cccgccgatc tcgccacact
       61 ctgccgcctc ggcgccgacg tgccgttctt cgccgccgga gccgcagcgg ctctggtgac
      121 gggcatcggc gaggtggtgc agccgctggg tgccgccgcg cccgaccatg ccatccttgt
      181 gcaccctggc ttcggcctgt cgactgccgc cgtcttcgac gagcttcgct cggccgacct
      241 cgctgcggca cgaccgaacg ggaatgacct gctggcgccg gcgctgcgcc tgcgcccaga
      301 gctcggtgac ctgatgcggc aggtgtcgga ggccggggcc gagccccacc tcactggttc
      361 gggatccacg atcttcgccc tcaccgacga ccccgagcgc gcggcggcca tcgcggccgg
      421 cctccgaagg acgggggtgc gtgtcacctt gacgcgcgtg cgcggaaagc cggcagagat
      481 ccttgagctc ggcgaggcgc cgcaggatgc tggcgccacg acgccgagca tgggatgatc
      541 gaggcccgat gctcgataaa ggagtcatga cggtccgcga cgaggtcctg accgatgccg
      601 cgcccgccgc gatcggccca tacagccagg caatgcgcgt cggcggtttc gtcttcaccg
      661 ccgggcaggt cggggccgag ccctctgacg gagcgctggc ggacggcgtc gcggcccagg
      721 ccgatcgtgc gctgcgcaac ctcgccgcgg tcctggcagc ggccggctcg agcttcgacc
      781 gcgtggtgaa gaccaccatc ttcctggtcg acatgggtga cttccaggcg gtcaacgagg
      841 tgtacgcccg tcacctgtcg ccaccattcc cggcccgttc gacgatcgcc gtcaaggcac
      901 tgccgaaggg tgcgttggtg gagatcgaga tgatcgcgct cgcctctgac gaggagcctg
      961 cacgatcgtg acagggagag cggcggtcgt tctggccgcc ggacagggca cgcggatgcg
     1021 ttcccacctg ccaaaggtgc tccacccgct ggccgggcga ccgatgctcg gccatgttct
     1081 cgctgcgctg gacaaggccg ggatcgagca gctggtggtc gtcgtcgggc atgatggcga
     1141 gcaggtcgag gcggtcgtcg gcggcaccgc cacgaccgtg cgacaggagc cgcagagggg
     1201 gaccgccgat gcagtgcgca tcggcctcga gcggctggac cccggaacgc agcaggtcgt
     1261 ggtggcgatg ggagatgcgc ccctggtgcc ggccgtgctg ttcgaggagc tcctctccgc
     1321 acaggccgcc ggagacgcct gcatcgcact ccttgcatcg cggccccacg atccgagtgg
     1381 ctatggccgc gtcgtgcgcg atgccggtgg ctcggtcgag gcgatcgtcg aggagaacga
     1441 cgccgatgcg cgggtgctcg agctgggcga ggtcaatgcc gggacctact gcttcgacgc
     1501 cggatggctg cgggccaaca tcggcgccgt gcctgcctca cgctccggcg agcattacct
     1561 gaccgacctc gtggcgatcg cggtctcgag cggccgcagc gtggtcgtgg tcaacgcgcc
     1621 gcgagcggag ctggcgatgg ggatcaacga ccggtccggg ctggcgactg ccgagcgggt
     1681 cctccgcagc gagatcgcag aacgccacat gcgcgacggg gtcaccatcg tcgatccgtc
     1741 gagcaccttc atcgacgcgg acgttcagat cggcgaggac gccaggatcg agccctggac
     1801 gatcctggcg ggcgccacca cgatcggccc caaagcggtg atcggtcccc aggcccaggt
     1861 cctcgattcg aagattggag cgcgaacgac gatctggtcc agcgtggtcg agtcatcggt
     1921 cgtggccgag gacgtgcaga tcgggccgta cagccacctc cggccgggct gcgagatcgg
     1981 gccgggctgc cgcatcggca acttcgccga gctcaagatg acccgcgtcg gcgccggaag
     2041 ccagatccat catctcagct acctcggcga tgccgatctc ggcgaggcgg tgaacatcgg
     2101 cgccgggacg atcaccgcca acttcgatgg gaaggtcaag catcggacca acatcggcga
     2161 cggggcgttc atcggggtcg acacgatgct gcgcgcgccg gtcagcgtgg ggcccggggc
     2221 acgcaccggg gcgggcgcgg tcgtgacccg cgacgtgccg gccggcgaga cggtcgtcgg
     2281 catgccggcg cgacggatcg ggacgcgccg acggcgcacg gaagttgccg ctgatcggaa
     2341 cccggaccca gggggcggca agccccgctc tgatgcctga gccaggcgtt ggtggcagcg
     2401 ccaccgtgaa ccccgtcctc gccctgctca tcgtcggggt gctggtcgtg gtcagcggct
     2461 tctttgccgc ctccgagctg tcgttgatca cggtcaagcg gtatcgcatc acgcagctcg
     2521 ccagcgaggg caatcgttcc gccaaggccg ccgaacggct gctggaggac ccgtcccgat
     2581 tcctggcgac gatccaggtg gcgctgacgt tcctgggctt cctggcatcc gccgttggcg
     2641 ccgtttcgtt ctctggccct gtggcggact tcattcggct gatcccgtgg gcaccgatca
     2701 aggaggcggc gccgaccatt gcgttcctcc tgaccaccat gatcatcgcg ctcgtgacga
     2761 tcgtgatcgg tgagcttgcc ccgaagaccc tggcgctcag cttcacggaa cggttcgcgc
     2821 ttgccgtggc ccggcccatc gccttcatgg ggaggctgct cagcccgttc gtctggctcg
     2881 tgagcctcat ctccggattc ctcgtgcgcg cgctcggcgg caagaaccgg ccacaggcgg
     2941 gctacctctc gaccgaggag ctgaagatgc tggtcgagac cggcagcgag cagggcggca
     3001 tcgaggagga cgagaaggag atgatccacg gggtcatcga gctcgccgat acccaggtcc
     3061 acgaggtgat ggtgccgcgg atcggaattc gcgcggtcga cgtcaacgat ccgatggagg
     3121 aggtgctgga cctgatcgtg cgggcgggtc actcccgcgt tccggtgtac gaggagaacc
     3181 tggacaacat catcggcatc ctctacgcca aggacctgct gccgtacctg aagggcaatg
     3241 gcggaggcgc cccagacatc gatatccgca agctggcgcg cccgccggtc tatgtgccgg
     3301 agaccaaggc cgtcgatgag ttgctgcacg agatgcaggt caccaagcgg cacattgcga
     3361 ttgtcgttga cgagtacggc ggcaccgccg gcctgatcac gctcgaggac gtggtcgagg
     3421 agatcgtcgg tgagatcgag gatgagtacg accgcgagga gtcgctggtg gagccactcg
     3481 aggaggaggg caaggtcgcc ttccgcgtcg acgggcgggt cagcatggat gacctgcgcg
     3541 acctgttcga cctctccgat accgaggagg aggacgaaga cgagtacgac acggtcggcg
     3601 gattcatcgt ccaccgcgtc ggacgcatcc cgctgccggg cgccgtggtg ccgttccgcg
     3661 acgtgacgct gacggtcgag gcggccgagc cgcgccgcat cgccaaggtg atcgccaccc
     3721 ggccgcgcga gccggaatcg gagacggggg aggtggcggc cgccgacgcc gattaggcgc
     3781 tgatcatgct ctcgacgagc gtgcgcaggt cctcgaggcg ctcgggcgga accggaagcg
     3841 accgcgcctc caccagggcc aggtcccggt agcgccgagc ctcctgctcg gtcgcagcgc
     3901 gcgcgccgga gcgttccagg atctcgagca cctcctcggt cagctcctcc gacatcggta
     3961 catcaccgtc gggtgggccg gagccatcgg tggcccggat caccggctgg tagatggcga
     4021 tcaggcgcgc gacgtcagcc gcggccgcgt tccgcatcgc ccacaccacc gggtacgtct
     4081 tcttgcgctt gcggatgtcg cccgccgccg ccttgcccga gtcggcgctg gcccagaagc
     4141 tgcccttcag gtcatcggcg atctggaatg ccatccccag gtcccgtccc agccgatggt
     4201 atgcgccgat cacctcttcg tcgtcgctgg ccatcatggc gctcgtctcg atcgaggcgc
     4261 cgatcagcgc ggcggtcttc ttggagatca tctccaggta ctcgtcgacg gtcacatcgg
     4321 tgcggcgctc aaagctgatg tccaggtact ggccctcgca cagggccagg caggtctcgt
     4381 cgtagacgcg catcacgtcg aggagcttct gcggctcgta ctcctcctcc ttcatcagcc
     4441 gatacagggc cagccgcgag agtgcgaaga gggcatcccc ggcattgatg gccagcggca
     4501 cgccccatag cgcccacagg gtgggccgat gccgccgctc gcgatcggaa tcctcgatgt
     4561 cgtcatggac caggctgaag ttgtgtccga gctcgaccgc cgccgctgcg gggagcgcct
     4621 tgcgatactc gccgccgaac gacttgaagg cgagcaggcc gagcagtggc cgcagccgct
     4681 tgccgcgcgg cccctccatc tggtcgagcc cgaggtggta ggcgagcatc tcgtacaggg
     4741 gtcgggcgct cccgtcgcgc tcgccgatga cggaccgcat ctcgtcctcg atctccacca
     4801 gcaggtcgtt aaactggctc atgacttcca cgacccaagg gtacccgagg tcgatccgcc
     4861 cgtgagccgg gcacgcctct acaagaccga ggggatcgtg ctgcgcagca tggatctcgg
     4921 cgaggccgat cgggtgctga cggtcctgac gccgcgcctc ggcaagctgc gtgtgatcgc
     4981 caaggggatc cgacggccac gctcccggct ggggggcggg ctcgagccgt tcagcgacgt
     5041 ccacctggtg ctggccgtag ggcggacctt cgacgtcgtc acccaggcct cgctggagga
     5101 tccgcacctg ggcctgcgtg acgatctcta ttcgaccgcg gcagcctggt atgtggtcga
     5161 gctggcggac cgcttctgcg aggggtcggc cgaatcgcac caggccttcg agctgctcgc
     5221 ccaggcgctg gcggcgctcg atgccgcccc cgaagcggtg tcgcgcgagg tcgtcgcgcg
     5281 ctggttcgag ctgcacctgc tcgaggcgat gggcttccgc ccagagctcg ggcagtgcct
     5341 tgactgcgga gtcgagatcg agccggacgg aaacgcctat tccccggtgg cgggaggcgt
     5401 catcgggccc gagtgcaccc acaccgcgct cgggacgcgg cagatctcgg ccgcggcact
     5461 gaaggtgatg cgttatctgc agcgctcccc gctgaccgaa gtccttcggc tgcgtctgtc
     5521 gagcccaacg cagcgggagg tcgagcatct cctccatgcg agcgtctcgg cggtgctcga
     5581 acgggagctg cgcagccgcg atttcctcga cgaggtcgcc ctgcgaggga tcccggcgcc
     5641 ggcctagcct ggatcgtcct gagttcggca gccaagccga tcggctgcgc tagactcgcc
     5701 gccatgcccg cgccgaacat ggagctcatc gtcagcctcg ccaagcgccg cggcttcgtg
     5761 ttcccgtcga gcgagatcta tggcggaatg ggcggcttct gggactacgg gccgctcggc
     5821 gtcgagctca agaacaacgt caa
//
LOCUS       DATCQR010000072         7161 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_34606, whole genome shotgun sequence.
ACCESSION   DATCQR010000072 DATCQR010000000
VERSION     DATCQR010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 7161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7161
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34606"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1395
                     /locus_tag="VIN32_02465"
     CDS             <1..1395
                     /locus_tag="VIN32_02465"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEY8921472.1"
                     /translation="GLGGILTVLSTLIFGLTVLPALLALLGPRVNRLRVPLPRSLRLI
                     EDDADAADRRQGHGVWAWIAARVMRRPLLVAIPVLAILLVAGSPFLGIQLSTGQNLAD
                     LPPVPSRIGFEVIADKFPGGESDPIAVVMTWPGVDLDGILSLERQAAIAEYTRFIRSI
                     DPQVNAVESALTPPPGMTDQQYLALLAQPQAERLAALNEYVAAWVGGDTTKLRVFSKY
                     VPDSDDGRALVDKIRSISPPNGTQILTGGLPSRSADFMRSFRGSVPIAILIVVLVTSV
                     VLFLTFGSVFLPVKAVVMSLISVTASFGALVWIFQEGHLKDLLGFETSHTIGAWEPII
                     MFAILFGLSMDYEVLLLSRIRERYLATGDNTRAVAEGIGITGGMITGAALIMVAVFAA
                     FGMSSITSIKALGVTMSLAVLIDATIVRGLLVPAFMRIMGRVNWWAPAWLQRAIARLG
                     LYEVGTQSADGPAR"
     gene            1392..1679
                     /locus_tag="VIN32_02470"
     CDS             1392..1679
                     /locus_tag="VIN32_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921473.1"
                     /translation="MTDSRRQRLDRLTDRWRARHEARVAAEGRPEADPEREARAHAFF
                     PWRTQSAAEYAATHGAAMVGYTYDAYSYADPELEAWLRELGELLRTRGRRC"
     gene            1811..2269
                     /locus_tag="VIN32_02475"
     CDS             1811..2269
                     /locus_tag="VIN32_02475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921474.1"
                     /translation="MSVTAGTDQLDQRLTLLHELAHWLGPGSRRRRGRVMHHDARFYA
                     IAFDLYRRHGVPDGEVLAREGGRYPSALRHARALGIPGADTAWLSRRGALRERRRLRA
                     PVRVLVGEHTVRLIRDGRWTRCGICGVRVVGPTLARLRRRSGRHVLLSAG"
     gene            2279..3466
                     /locus_tag="VIN32_02480"
     CDS             2279..3466
                     /locus_tag="VIN32_02480"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY8921475.1"
                     /translation="MDAALPLAGIRVLDCSRVYAGPIASMLLGDLGAEIWKLEPPNGD
                     ETRGWGPPYWGDPAGRRSAYFASVNRNKRSLVVNLKTDAGRALLDRLAAMCDVLLHNY
                     QPSVATELGLDADRLRAAHPHLVVSTIGGFPGEGDAAERPAYDLVAQAFSGLMSVTGD
                     PYGGPMKVGVALLDLNAGLQAAIGALAGLVARERGREGGLHASVSLIESGVASLTNVL
                     GHHLATDEEPRRWGTGHPDIVPYQVFAARDGHLVVAVGNDAQFSRLLAVLDLELDSRF
                     ASNEGRLAGRDGLVSLLAVRIGDWSRDELVGALTEADVPAGPVNSVSEALAAMEAAHD
                     GEWSQARDGMRLAPATIRIDGQRLPLRATPPRLGADTDGILQEAGLDAGEIAALRAAE
                     VIG"
     gene            complement(3793..4119)
                     /locus_tag="VIN32_02485"
     CDS             complement(3793..4119)
                     /locus_tag="VIN32_02485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921476.1"
                     /translation="MNTIIGRENLSGQRTGCPTPHDVCRSADGNGTQVSASGWQVSDH
                     ASSASQINLLHDGRWGCTAATEDHHGAIEGDASGVVNWHRQFAQSANLASTRIKRKNL
                     TRRRAG"
     gene            4165..4533
                     /locus_tag="VIN32_02490"
     CDS             4165..4533
                     /locus_tag="VIN32_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921477.1"
                     /translation="MAETGGRIYVIGGAGAAGERRDIQRVDIATGEVQVIGQMPRPIS
                     HASAMIIDGRIFVVGGRSAGAAQDAIWQLDAVTGATQLVARLPQPASDFALAVVGGVG
                     YAIGGETDTQVASIVAIVVE"
     gene            complement(4562..5950)
                     /locus_tag="VIN32_02495"
     CDS             complement(4562..5950)
                     /locus_tag="VIN32_02495"
                     /inference="COORDINATES: protein motif:HMM:NF044870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HEY8921478.1"
                     /translation="MRCRSLLAALFLLAACGSPMSPTPSVVPSATLAPSPTAAPTATP
                     QPTATPPRVTVGQVARVMVADLQVKDQPPQFASSQQVGVLGQGELVTVTDGPNVVADQ
                     RWWKVEVAALVGWVPDLVAGQAALLDVAEPAARDWNVWAATTSSTLPAAVASLPARLY
                     VPNEADGTVTVVDVAKRSVIDVLRAGVLPEHVTPDWDLSRLYVSNYGSTFMTAMDAQT
                     GKLIDPIQAPFAYNLYFTPDGTTAIVMAEEVDRVDFYDRATWQPLGQLPIPWRGIDHA
                     DFSAGGRYLMASTEYAGHVLKIDVVTRSIVGDLQVGGLPIDTKLSPDGLVFYNANQGR
                     HGVSIIDPIKMQEIAFLPTGTGAHGMAISRDTKSLYVSNRLVGTISVISFATRQVVAT
                     WAVGGTPDMLQVSPDGTELWVSNRYGTTVTVINTATGAVIDTIEVGRQPHGLTYFPQP
                     GRFSIGHNGVYR"
     gene            complement(5984..>7161)
                     /locus_tag="VIN32_02500"
     CDS             complement(5984..>7161)
                     /locus_tag="VIN32_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEY8921479.1"
                     /translation="SSLVMLMTPGLAFFYGGLVSRRNVLTIMMQSFVSMGWTTVIWFA
                     VGYSLTFSGDVGGVIGNLDMAFLRGVSLDTPFPGTQIPLLVHVAYQMMFAIITPALIT
                     GAFAGRITFKAYLAFLTLWLLFVYFPFAHMIWGGGLLQKWGVLDFAGGIVVHNTAGLA
                     ALASVLYVGRRKVKDSGAHSIPLVALGTGLLWFGWYGFNAGSEFKVDAVTAVAFLNTD
                     VAASFAAITWLLIGWWREKRPTFVGLLTGAVAGLATITPAAGYVSPVTAVIIGIAAGA
                     VCFFAVALKNRLQLDDALDVWGVHGVGGFLGIVLLGVFATTAFNPAGTDGLIAGNGWF
                     FLKQLVAVAVSSVWAFGFTYGMLWLIDRFTPVKVEESAEQSGLDAALHGETAYLETG"
BASE COUNT         1103 a         2395 c         2499 g         1164 t
ORIGIN      
        1 gggctgggcg ggatcctgac ggtgctgtcg acgcttatct tcgggctgac ggtgctccct
       61 gcgctgctgg ccttgctcgg cccccgggtc aaccggctgc gggtgccgct cccacgctcg
      121 ctgcgcctga tcgaggacga cgcggacgct gccgaccggc gccaggggca cggcgtctgg
      181 gcctggatcg ccgcccgcgt catgcgccgg ccactgctcg tcgcgatccc cgtgctggcg
      241 atcctgctcg tcgccggctc gccgttcctg gggatccagc tctcgacggg acagaacctc
      301 gccgacctcc cgccggttcc atcccggatc ggtttcgagg tgatcgccga caagttcccc
      361 ggcggtgaga gcgacccgat cgcggtggtc atgacctggc caggggtcga cctggatggc
      421 atcctctcgc tggagcgaca agcggccatc gcggagtaca cgcgcttcat ccgatccatc
      481 gacccgcagg tgaatgctgt ggagagcgct ctcacgcctc cgccagggat gacagaccag
      541 caatacctcg ccctgctcgc acaacctcag gcggagcggc tggcggccct caatgaatac
      601 gtcgcggcgt gggttggggg tgacacgacc aagctgcgtg tcttctcgaa gtacgtcccc
      661 gattccgacg acggtcgcgc cctcgtcgac aagatccgct cgatttcgcc tccgaatggg
      721 acgcagatcc tcactggcgg cctgccgtcc cgctctgccg acttcatgcg cagcttccgc
      781 ggatcggtgc cgatcgcgat cctgatcgtc gtcctcgtca ccagcgtcgt cctgttcctg
      841 accttcggat cggtcttcct gccggtcaag gcggtcgtca tgagcctgat cagcgtcacg
      901 gccagttttg gggcgctggt ctggatcttc caggagggac acctcaagga cctgctcgga
      961 ttcgagacct cccacacgat tggcgcgtgg gagccgatca tcatgttcgc gatcctgttc
     1021 ggactgtcga tggactacga ggtcctcctc ctgtcgcgca ttcgcgagcg ctacctcgcc
     1081 accggcgaca acacccgcgc ggtggccgag gggatcggca tcaccggcgg gatgatcacc
     1141 ggcgccgcac tgatcatggt cgccgtcttc gccgcgttcg ggatgtcgag catcacttcg
     1201 atcaaggcat tgggcgtgac gatgtcgctc gccgtgctga tcgacgcgac gatcgtgcgc
     1261 ggcctgctgg tgcccgcctt catgcgaatc atgggacgcg tcaactggtg ggcacccgcc
     1321 tggctgcagc gagccatcgc ccggctcggc ctgtacgagg tcggcacgca atcggccgac
     1381 ggccccgctc gatgaccgac tcgcgccggc agcgcctgga ccgattgacg gaccgatggc
     1441 gcgcgcgcca cgaagcccgc gttgcggcgg aagggcggcc cgaggccgac cccgagcgcg
     1501 aggctcgcgc gcacgcgttc ttcccgtgga ggacccagtc ggccgccgag tatgcggcga
     1561 cccatggcgc ggcgatggtg ggctacacat acgacgccta ctcgtacgcc gatccagagc
     1621 tcgaggcgtg gctgcgtgag ctcggggagc tgcttcgcac gcgaggccgt cgctgctgat
     1681 ggctcctccc tgggccagcg agatcgtgca tggcgagtgc gcggccgcgg gcgtggcaga
     1741 accgatgatt cgatggcgac acgcgcggcg ctcggggtcc tcgggcatga cacgtcggtc
     1801 ggcgggcagc gtgtccgtca ctgccgggac cgatcagctt gaccagcgcc tgacgctgct
     1861 ccacgagctg gctcactggc tcggccccgg gtcgcgacgc cgccgaggac gcgtcatgca
     1921 tcacgatgcg cggttctacg ccatcgcctt cgacctgtac cggcgccacg gcgtccccga
     1981 tggcgaggtc ctggcccgcg aaggcggccg gtatccgagc gccttacggc atgcgcgagc
     2041 gctggggatc ccaggcgccg acaccgcgtg gctcagtcga cggggtgcgc tgcgcgagag
     2101 gcgtcggctg cgcgctcccg tgcgcgtgct ggttggcgag cacacggtcc ggctgattcg
     2161 cgacgggcgc tggactcgat gtggcatctg cggcgtacgg gtggtcgggc cgacgcttgc
     2221 ccgcctccgc cgccgcagcg gccgccacgt cctgctgagc gcgggctagg ggacgccggt
     2281 ggacgccgcg ctgcccctcg ccggcatccg cgtgctcgac tgctcgcgcg tctacgccgg
     2341 gcccatcgcc agcatgctgc tgggcgacct gggagcggag atctggaagc tcgagccgcc
     2401 gaacggcgac gagacgcgcg gctggggacc gccgtactgg ggcgacccgg cgggccggcg
     2461 gagcgcgtac ttcgcatcgg tcaaccgcaa caagcgcagc ctggtcgtga acctcaagac
     2521 cgatgcgggc cgcgccctgc tcgaccgcct cgctgccatg tgcgacgtac tcctgcacaa
     2581 ctaccagccg tcagtcgcca cggagctcgg cctcgatgct gatcgccttc gcgcggcgca
     2641 tccgcacctc gtggtgtcga ccatcggggg cttcccggga gagggcgacg ccgctgagcg
     2701 ccccgcctac gacctggtgg cgcaggcgtt cagcgggctc atgtcggtca cgggcgatcc
     2761 ctacggcggc ccgatgaagg tcggcgtcgc gctgctggac ctgaatgcgg ggttgcaggc
     2821 cgcaatcggt gcgctggccg ggctggttgc ccgagagcgc ggccgcgagg gcggactgca
     2881 cgcctcggtc agcctgatcg agtcgggcgt cgccagcctg accaacgtgc tggggcatca
     2941 cctggcaacg gacgaggagc cgcgccgctg ggggaccggt caccccgaca tcgtgccgta
     3001 ccaggtcttc gctgctcgcg acggccacct ggtggtggcg gtcggcaacg atgcgcagtt
     3061 ctcgcgcctg ctcgccgtgc tcgacctgga gctggactcg cgctttgcca gcaacgaggg
     3121 caggctcgcg ggacgcgacg ggctcgtttc cctgttggcc gtgcgcatcg gggattggtc
     3181 gcgcgacgag ctcgtcggcg ccctgaccga ggccgatgta ccggccggcc cggtcaactc
     3241 ggtcagcgag gccctggcgg ccatggaggc ggcgcatgac ggggagtgga gccaggcacg
     3301 cgatggcatg cgactggccc cggccacgat ccggatcgat gggcagcgcc tgccattgcg
     3361 cgcaacaccg ccgcgcctgg gggcagacac cgacgggatc ctgcaggagg cggggctcga
     3421 cgcgggcgag atcgccgcgc tccgcgcagc ggaggtgatc ggctgagcga gacaccgccg
     3481 gctcatcctc gggctcgatc ccggttgctg gcgacgctgg tcgcgattgg cgcgacggtc
     3541 atgagcgcgt gcggtccggt cacgcctccc tcgtcactaa cgcccctggg atcgacgaca
     3601 gcgcggccgg cctcgagtcc gagcccgctc gcgaagggga ccggagcgtc tggagcaccg
     3661 gcgacgaccg gcgccagccc cgtgtgggca gtggttcacg caacggcgct gccgactcgt
     3721 ctcccgaccg ggctgagtcg ttctgtcgcg atcgttgtcg gcagcagcat cgacatctat
     3781 ggcggctttg cctcaaccgg ctcgacgacg ggtgagattc ttgcgtttga tccgggtgga
     3841 ggccagattg gcgctctggg cgaactggcg gtgccagttc acgacgccgc tggcgtcgcc
     3901 ctcgatggcg ccatgatggt cttcggtggc ggcagtgcag ccccaacgtc cgtcgtgcaa
     3961 caggttgatc tggctggcgc tgctcgcgtg gtcggaaacc tgccaaccgc tcgcgctgac
     4021 ctgggtgccg ttgccatcgg cacttctgca tacgtcgtgg ggggtggggc atccggtgcg
     4081 ctggcccgac agattctcgc gaccgatgat ggtgttcatt ttcgcgccgt ggcgagcctc
     4141 gtttcagccg tccggtacgc ggccgtggct gagacaggcg gacggatcta tgtgatcggc
     4201 ggagccgggg cggccggcga gagaagggac attcagcggg tcgacatcgc gactggcgag
     4261 gtgcaggtga tcgggcagat gccaagaccg atctcccatg catcagcgat gatcattgac
     4321 ggacggatat tcgtcgtcgg cggccgaagc gcgggcgctg cgcaagacgc catctggcag
     4381 ctcgacgccg taacgggcgc gactcagctg gtggctcgtc tgccgcagcc ggcctccgac
     4441 ttcgcgttgg ctgtcgtcgg cggagtggga tacgcgatcg gtggggaaac cgatacgcag
     4501 gtcgcctcga tcgtggctat cgtcgtcgaa tgacggttga gacggcgtcg gcggggtgcc
     4561 tctatcggta gacgccgttg tgaccgatgc tgaaccggcc cggctgcggg aaataggtca
     4621 agccgtgggg ttgccgccca acctcgatcg tgtcgatgac ggctcccgtg gcggtgttga
     4681 tcaccgtaac ggtcgttcca tagcggttgc tcacccacag ctcggtgccg tccggggaga
     4741 cctgcagcat gtccggcgtg cccccgaccg cccacgtggc gaccacctgc cgggtggcga
     4801 agctgatcac ggagatggtc cccaccagcc ggttgctgac gtagagggac tttgtatcgc
     4861 ggctgatggc catgccgtgc gcccccgtcc cggtcggcag gaaggcgatc tcctgcatct
     4921 tgatcgggtc gatgatcgac accccgtggc ggccctggtt ggcgttgtag aagaccagcc
     4981 cgtccggcga cagcttggtg tcgatcggca gcccgccgac ctgcaggtct ccgacgatcg
     5041 atcgggtcac gacatcgatc ttgagcacgt ggccggcgta ttcggtgctg gccatcaggt
     5101 atcggcctcc tgccgagaag tcggcatggt cgatgccgcg ccaggggatc ggcagctgac
     5161 cgaggggctg ccaggtggcg cgatcgtaga agtcgacccg atcgacctcc tcggccatca
     5221 cgatggcggt cgtcccgtca ggtgtgaagt acaggttgta cgcgaacggc gcctggattg
     5281 gatcgatgag cttgcctgtc tgggcatcca tggcggtcat gaatgttgag ccgtagttgc
     5341 tcacatacag ccgcgacagg tcccagtccg gcgtcacgtg ctccggcagg acccccgctc
     5401 gcagtacgtc gatcacggac cgcttggcca cgtccacgac cgtgacggtg ccgtccgcct
     5461 cgttcgggac gtagagccgc gcgggaagag acgcgacagc ggcggggagc gtcgagctgg
     5521 tggtggcggc ccatacattc cagtcgcgtg ccgccggctc cgcgacatcc aggagggcag
     5581 cctgcccagc gacgagatcg gggacccagc cgaccagggc agccacctcg accttccacc
     5641 atctctggtc cgcgacgacg ttcgggccgt cggtcacggt caccagctcg ccctgcccca
     5701 gtacgccgac ctgctgggag cttgcaaatt ggggcggctg atccttcacc tgcagatcgg
     5761 cgaccatgac ccgtgccacc tgcccgaccg tcacgcgcgg gggcgtggcc gttggttgcg
     5821 gcgtcgcagt cggcgcagca gtcgggctcg gagccagcgt tgccgacggg accaccgacg
     5881 gggtcggcga cattgggctg ccgcacgccg ccaggaggaa cagcgcggcc agcaggctgc
     5941 ggcatctcac cgccgaacgg tactccgtcg aaccgtaggc gggtcagcca gtttcgaggt
     6001 acgcggtctc cccgtgaagc gcagcgtcca ggccgctctg ctcggccgat tcctcgacct
     6061 tgaccggggt gaatcggtcg atcagccata gcatcccgta ggtgaagccg aaggcccaca
     6121 ccgatgagac ggccacggcg accagctgct tgaggaagaa ccacccattg cccgcgatga
     6181 gcccatccgt tccggctgga ttgaaggcgg tcgtggcaaa gactcccagc agcacgatcc
     6241 ccaggaagcc gccgacgccg tggactcccc agacgtccag cgcgtcatcc agctggaggc
     6301 ggttcttgag ggccaccgca aagaagcaga cggcccctgc cgcgatcccg atgatcaccg
     6361 cggtgacggg cgagacgtac ccggcggctg gcgtgatcgt cgccaggcct gcaacggcgc
     6421 cggtcagcag ccccacgaag gtcggccgct tctcccgcca ccagccgatc agaagccagg
     6481 tgatcgccgc gaacgatgcg gcgacatcgg tgttcaggaa ggcgaccgct gtgaccgcgt
     6541 cgaccttgaa ctcgctgccg gcattgaatc cataccagcc gaaccacaac agtcctgtgc
     6601 caagggcgac gagggggatg ctgtgcgcac cggagtcctt caccttgcgg cgtccgacgt
     6661 acaggaccga tgccagggcc gccaggccgg cagtgttgtg aaccacgatc cccccggcga
     6721 agtcgaggac gccccatttc tggaggaggc cgccgcccca gatcatgtgg gcgaagggga
     6781 aatagacgaa aaggagccag agggtcagga atgcgaggta cgccttgaac gtgatgcggc
     6841 cggcgaaggc gccagtgatg agcgcggggg tgatgatcgc gaacatcatc tggtatgcca
     6901 cgtggacgag gagtgggatc tgggtgcccg gaaatggcgt atccaggctg acgccgcgca
     6961 ggaaggccat gtccaggttg ccgatgacgc cgccgacgtc gccggagaag gtcaatgaat
     7021 agccgaccgc gaaccagatg acggtcgtcc agcccatcga gacgaaggac tgcatcatga
     7081 tcgtcagcac gttcctgcgg ctgaccaggc ccccgtagaa gaacgcaagc cccggggtca
     7141 tcagcatgac caggctgctg c
//
LOCUS       DATCQR010000073         6466 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_143203, whole genome shotgun sequence.
ACCESSION   DATCQR010000073 DATCQR010000000
VERSION     DATCQR010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6466
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143203"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..390)
                     /locus_tag="VIN32_02505"
     CDS             complement(<1..390)
                     /locus_tag="VIN32_02505"
                     /inference="COORDINATES: protein motif:HMM:NF016005.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEY8921480.1"
                     /translation="MTASDDLGAFLEALLFIAERPLTGAELADLAGVQRPQAEAALAS
                     LARDLAEGRRGLRLQRLDDAWQLATAPEVGERLAAYAAREEARLTPAALEALAVVAYR
                     QPCTRGEVERVRGVDSDYVLRSLLHRRL"
     gene            complement(387..1172)
                     /locus_tag="VIN32_02510"
     CDS             complement(387..1172)
                     /locus_tag="VIN32_02510"
                     /inference="COORDINATES: protein motif:HMM:NF014654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEY8921481.1"
                     /translation="MPTELDPIEIARPAVDAVAVRIAGATEPDFEVQLPTFSGPLAVL
                     LHLIESRQLEVLTVPLAELADAYVAHLAEHPVDAAQLAEFVAVAAQLILIKSRSMLPG
                     EVLPVGPDVADEPDEEELRRRLVEYRALRDVARALGERDLVTPMQRREPRESDLPVAS
                     LAPLPVALLADAMRALAAIPEPEPAPPEIMAREITIAMQITVLRAALAAGGRVVLQQL
                     LASCRSRTEATVTLLATLELVRRRQVRVKQRELFGPIVLETLA"
     gene            complement(1177..1665)
                     /locus_tag="VIN32_02515"
     CDS             complement(1177..1665)
                     /locus_tag="VIN32_02515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921482.1"
                     /translation="MGHRVGQFIGHLTARVAPGEIERARPLLPDTGWALFSAMPTADR
                     RHALDVAARLAAVGQRDPDLLAAALLHDAAKGHRMRLWHRVAGVLLEAVAPRTLARLA
                     LPAERSWRYPFHLYLQHAALSADAALAAGCSQRTVAFIRGSTEPADAPLAAALRRADE
                     GR"
     gene            complement(1655..2287)
                     /locus_tag="VIN32_02520"
     CDS             complement(1655..2287)
                     /locus_tag="VIN32_02520"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEY8921483.1"
                     /translation="MLFGSLSLPELVGIAIALLVGITFHEFSHAFVADQLGDHRPRAL
                     GRVSLNPLRHLDPLGSLFFLLAGFGWGKPVPVNAYALRPGRQGLTFVALAGPIANFTV
                     AAVLAIIFRALQLAGMLDAPFVRDVVGLTVYFNVALGLFNLIPIPPLDGYNVVLPLLP
                     PRQSFLVQRYAQYGYIALLLLLVLSYGPGNGPLGWLFGLASTIAGLMLGT"
     gene            complement(2287..3171)
                     /locus_tag="VIN32_02525"
     CDS             complement(2287..3171)
                     /locus_tag="VIN32_02525"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEY8921484.1"
                     /translation="MEEPRRSLSDGVFGAELERRIDEAAAAIRDRLALTPELAAPRLA
                     IVLGSGLGGVAELLDPQPRIVIPYREIPNVPAAAVAGHAGELVAGFASGIPVLLLSGR
                     AHAYEGWSHRQTTLLLRACLSLGIETLVVTNASGGINPAFDPGDVMLIADVINLSGDN
                     PLTGPNLDRFGPRFVSMTDALDADLRTLARTAAERAGVALREGVYLMLAGPTYETRAE
                     LRMLRTLGADAVGMSTVPEVLVARHHGVRVLGFSLVTNKATPEMEGEVTHEEVLAMGP
                     IGAARLSSVLSELLPELG"
     gene            complement(3172..3954)
                     /locus_tag="VIN32_02530"
     CDS             complement(3172..3954)
                     /locus_tag="VIN32_02530"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEY8921485.1"
                     /translation="MSYARNLLSRGEEVVFESRQHWFAVLGATWLFVLGAILALAVLI
                     WSSTSNSTQLIQILQIVSLVALIVSLALIAIKVWSWRNQEYLVTTRRLIKAEGVFNKE
                     MADSSLEKINDAHLTQSWVGRIFDFGTLDILTAADESAGIQDYYMLADPIRFKIAMLN
                     QKEKLERPDLAPPPRMQASSGAPAMQRAEPMPPRAGSDRVAQVPMEASHSAPPAAAAP
                     AAAPSAADQLTATLESLAALRDRGLITPEEYEAKKRDLLERM"
     gene            complement(3974..4873)
                     /gene="xerD"
                     /locus_tag="VIN32_02535"
     CDS             complement(3974..4873)
                     /gene="xerD"
                     /locus_tag="VIN32_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020014817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEY8921486.1"
                     /translation="MADAAVDPALDAAIRAFLDEMQVERGLSPLTVAAYRRDLAQFAA
                     VAGTAWRNDPEPVRDFVNALRRDGRRPSTQARKVAAIRSFYGFALREELADRDVAGLL
                     DAPKAGSYLPDVLSAESVASILDAPPSDDPVGIRDRAILELLYACGLRVSELTGLDTD
                     RLDLPGLQVRVIGKGNRERRVPMGDEARERIQQYLAGPRAAWTAGRPTPAVFLSQRGA
                     RLARESVWRLVRRWAAVAGVQTRVTPHTFRHSFATHLLEGGADLRVVQTLLGHASIST
                     TQLYTHLTGERLREVYARAHPRA"
     gene            complement(4882..5718)
                     /locus_tag="VIN32_02540"
     CDS             complement(4882..5718)
                     /locus_tag="VIN32_02540"
                     /inference="COORDINATES: protein motif:HMM:NF039482.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HEY8921487.1"
                     /translation="MSRFGFAFNPTNPQARATLERARAWCAANGVDAWESEADDGDRI
                     TAECTGSDLVCVLGGDGTFLRAARGIGDSAAPTLGVNLGRVGFLAKVETDDLEQALAK
                     VASGDFALEDRFRIEATLQRANGERERHACLNEVVVARGPRVRMIQVEVEVSGSHLAT
                     WVSDGVVVATPTGSTAYSFSAGGSVLDPRLRNMIITSVAGYLSPLRSVVAGEDHVVRL
                     MLRQAVSGATISIDGQWDLPMAVGETVEVRALPVPLRMVELSGGTPFYDLLRTKASLL
                     PY"
     gene            complement(5715..6035)
                     /locus_tag="VIN32_02545"
     CDS             complement(5715..6035)
                     /locus_tag="VIN32_02545"
                     /inference="COORDINATES: protein motif:HMM:NF013561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HEY8921488.1"
                     /translation="MKRKPAAAAEQLSVFEELAALPEPIHEPAPDTMRQIVSLRMPDA
                     LLERVEEVARGRGVSPSSLMRALIAAGLDRSYAAVTPDELTAELAALRKGIDEIATRL
                     RTSE"
     gene            complement(6058..>6466)
                     /locus_tag="VIN32_02550"
     CDS             complement(6058..>6466)
                     /locus_tag="VIN32_02550"
                     /inference="COORDINATES: protein motif:HMM:NF013856.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEY8921489.1"
                     /translation="VSMERTNLRALRHLPEPIDLATLDLSFTSLSRVLPAVGALLRGE
                     GQVIALVKPQFEAGRDAIPRGGVVRDPATHRDVLLQFGADASGAGFSPTAVIASPIVG
                     ADGNREFLALLRPGDGPAAQWSTEVDRVAPSPV"
BASE COUNT         1014 a         2281 c         2276 g          895 t
ORIGIN      
        1 cagccgccga tgcaggaggg atcggagcac gtagtcggag tcgacgccgc gcactctctc
       61 cacctcgccg cgggtgcacg gctggcggta ggccaccacc gccagcgcct cgagcgccgc
      121 cggcgtgagg cgcgcctcct cacgggcggc gtaggccgcc agccgctccc cgacctctgg
      181 ggcggtggcg agctgccagg catcgtccaa acgctgcagg cgaagccccc gtcggccctc
      241 agccaggtcc ctggcgaggc ttgcaagtgc tgcctctgcc tgcggccgct gcacgccggc
      301 caggtcggcc agctccgcac cggtcagcgg tcgctcggcg atgaacagca gcgcctccag
      361 gaaggcgccg aggtcgtcgg aggcggtcac gccagcgtct cgagcacgat cggcccgaac
      421 agctcgcgct gtttgactcg gacctgccgg cgccttacca gctccagggt ggccagcagc
      481 gtgaccgtgg cctcggtgcg cgaacggcag gaggccagca gctgctggag cacgacccga
      541 ccgccggccg cgagggcggc gcgaagcacc gtgatctgca tcgcgatcgt gatctcacgg
      601 gccatgattt cgggtggcgc cggctccggc tcggggattg cggccagggc gcgcatcgcg
      661 tcggcgagca gggccaccgg caggggcgcc aggctggcca ccggcaggtc cgattcgcgg
      721 ggctcgcggc gctgcatcgg cgtcaccagg tcccgctcac caagggcgcg agccacgtcc
      781 cgcagggccc gatactcgac caggcgtcgg cgcagctcct cctcatcggg ctcgtcggcg
      841 acgtccgggc cgaccgggag cacctcaccg ggcagcatgc tgcgggattt gatgaggatc
      901 agctgggcgg ccacggccac gaattcggca agctgggcag cgtcgaccgg atgctcggca
      961 aggtgagcga cgtaggcgtc cgccagctcg gccagcggca cggtcaggac ttcgagctgg
     1021 cgcgactcga tcaggtgcag caggacggcc agcgggcctg agaaggtggg cagctgcacc
     1081 tcgaagtcgg gctccgtcgc gcccgcgatg cgcacagcga cggcgtcgac cgctggccgg
     1141 gcgatctcga tcgggtcgag ctcggtcggc atcggctcag cgcccctcgt ccgcgcgacg
     1201 caacgccgca gccagcggcg cgtccgccgg ctcggtggaa ccccggatga atgccacggt
     1261 gcgctggctg cagcccgccg caagggccgc gtcggccgag agcgccgcgt gctgcaggta
     1321 caggtggaac ggatagcgcc acgaccgctc ggcgggcaac gccaggcggg ccagcgtgcg
     1381 cggcgcgacc gcttccagca ggacgcccgc gacccggtgc cacagtcgca tgcggtgccc
     1441 cttggccgcg tcgtgcagca gcgcggcggc gagcagatcc ggatctcgct ggcccacggc
     1501 agccagtcgt gcggcgacgt cgagggcatg ccgccggtcc gcggtcggca tcgccgagaa
     1561 gagcgcccac cccgtgtcgg gcagcagcgg gcgcgcccgc tcgatctcgc ccggagcgac
     1621 gcgggccgtc aggtggccga tgaactgccc gaccctatgt cccaagcatc agccccgcga
     1681 tcgtcgaagc caggccgaag agccatccca gcgggccgtt tccagggcca tacgagagca
     1741 ccagcaggag gagcagcgcg atgtagccgt actgggcgta gcgctggacc aggaacgact
     1801 ggcgcggggg aaggagtggc agcaccacgt tgtaaccgtc cagcggcggg atcgggatca
     1861 ggttgaagag gccgagcgcg acattgaagt agacggtcag ccccaccacg tcacgcacga
     1921 agggggcgtc cagcatgccc gccagctgca gcgcgcggaa gatgatggcc agcaccgccg
     1981 ccaccgtgaa gttggcgatg ggaccagcca gcgccacgaa ggtgagtccc tggcgtcccg
     2041 gccgcagggc gtaggcgttc acgggcaccg gcttccccca accgaatccg gcgagcagga
     2101 agaagagcga gcccagcgga tcgaggtgcc gcagagggtt caggctcacc cgaccgagcg
     2161 cccgcgggcg gtggtcgcca agctggtccg cgacgaatgc gtggctgaac tcgtggaacg
     2221 tgatccccac cagcaacgcg atggcaatcc ccaccagctc cggcagtgag agcgacccaa
     2281 agagcactac ccgagctccg gcaggagctc actgaggacc gatgagagtc gcgcggcacc
     2341 gatcgggccc attgccagga cctcctcgtg agtcacctcg ccctccatct ccggcgtcgc
     2401 cttgttcgtc accaggctga agcccaggac tcggactccg tggtgtcgtg caaccagcac
     2461 ctccggaacc gtcgacatgc cgacggcgtc ggcgccaagg gtacgcagca tccgcagctc
     2521 cgcgcgtgtc tcgtaggttg gtcctgccag catgaggtag accccttccc gcaaggccac
     2581 cccggcccgc tccgcggccg tccgggccag ggtgcgcaga tccgcgtcga gcgcatcggt
     2641 catggagacg aagcgcggcc cgaagcgatc gaggttgggc cccgtcaatg ggttgtcgcc
     2701 ggacaggttg atgacgtcgg cgatgagcat cacgtcaccg gggtcgaagg ccggattgat
     2761 ccctcccgac gcgttggtca ccaccagcgt ctcgatcccc aggctcaggc acgctcgcag
     2821 caggagggtc gtctggcggt gcgaccagcc ctcgtaggca tgtgcccggc cggagaggag
     2881 caggaccggg atccccgatg cgaagccggc cacgagctcg ccggcatgcc cggccaccgc
     2941 ggccgccggg acgttcggaa tctcgcggta cgggatgacg atccgcggct gcggatcgag
     3001 gagctcagcg acgcccccga gccccgagcc gaggacgatc gccaggcgag gagccgcaag
     3061 ctcgggtgtg agggcgaggc gatcgcggat ggccgctgct gcctcatcga tgcgccgctc
     3121 cagctccgct ccgaagaccc cgtcggagag ggagcggcga ggctcctcca cctacatccg
     3181 ctccagcagg tcgcgtttct tggcctcgta ctcctcgggc gtgatgaggc cgcgatcgcg
     3241 aagcgcggca aggctctcga gggtcgccgt cagctggtcg gcggcggagg gagccgctgc
     3301 gggcgctgcg gcagcaggtg gagcagagtg tgacgcctcc atcgggacct gcgccacgcg
     3361 gtcggatccg gcacgcggcg gcatcggctc ggcgcgctgc atggccggag cgccgctgct
     3421 cgcctgcatg cgaggaggcg gcgccaggtc gggccgctcg agcttctcct tctggttgag
     3481 catcgcgatc ttgaagcgga tcgggtccgc cagcatgtag tagtcctgga tcccggccga
     3541 ctcgtcggcg gcggtgagga tgtcgagcgt gccgaagtcg aagatgcggc cgacccagct
     3601 ctgggtaagg tgcgcgtcgt tgatcttctc caggctcgaa tcggccatct ccttgttgaa
     3661 gacgccctcg gccttgatca gccgacgcgt ggtgaccagg tactcctggt tgcgccagga
     3721 ccacaccttg atggcgatga gcgccaggga cacgatgagc gccaccagcg acacgatctg
     3781 caggatctgg atgagctggg tcgaattgct cgtcgagctc cagatcagca ccgccagggc
     3841 cagaatcgcc ccgagcacga agagccaggt ggcgccgagc acggcgaacc agtgctggcg
     3901 gctctcgaag acgacctcct cgccgcggct gagaaggttc cgggcgtaac tcatcagagt
     3961 gctccgcttc tgctcaggct ctgggatgcg cccgcgcgta gacctcgcgg aggcgttctc
     4021 cggtcaggtg cgtgtagagc tgggtggtgc tgatgctagc atgaccaagg agcgtctgga
     4081 ccacccgcag gtcggctccc ccctcgagca gatgggtggc aaagctgtgc cgaaacgtgt
     4141 gcggcgtgac ccgcgtctgc accccggcga cggcagccca gcgtcgcacc aggcgccaga
     4201 ccgattcacg cgccagccgc gctccccgct ggctgaggaa gacggctggg gtgggccgtc
     4261 ccgcggtcca ggccgcgcgt ggaccggcga ggtactgctg gatccgctct cgggcctcgt
     4321 cccccatcgg cacccggcgt tcgcggtttc ccttgccgat gactcgcacc tgcagcccgg
     4381 gaagatccag ccggtcggtg tccaggccgg tcagctcgct gacccgcagg ccgcaggcgt
     4441 agagcagctc caggatcgcc cgatcgcgaa tcccgaccgg gtcgtcggag ggtggcgcat
     4501 cgagaatgct ggcgaccgat tccgcgctca gcacatccgg caggtagctt cccgccttcg
     4561 gagcatccag caggcccgcc acgtctcggt cggccagctc ctcgcgcagc gcgaagccgt
     4621 agaagctacg gatggcggcc accttgcgcg cctgggtgct tggccgtcgc ccatcacgcc
     4681 gcagcgcgtt cacgaaatcg cgcacgggct ccgggtcgtt gcgccaggcc gtgccggcga
     4741 ccgccgcgaa ctgggcgagg tcgcgtcgat aggcggccac ggtcaacggg gagaggccgc
     4801 gctcgacctg catctcgtcc aggaacgcgc ggatcgccgc gtccagcgcc ggatcgacgg
     4861 ccgcgtcggc cattcgtcgg atcagtacgg cagcagtgag gccttggtgc gcagcaggtc
     4921 gtagaacggc gtgccaccgc tcagctcgac catgcgcagc ggcaccggca gcgcgcgcac
     4981 ctccaccgtt tcaccaaccg ccatcggtag atcccattgc ccgtcgatgc tgatcgtggc
     5041 acccgagacc gcctgccgca gcatcagccg cacgacgtga tcctccccgg ccacgacact
     5101 gcgcagtggc gacaggtagc cggcgaccga ggtgatgatc atgttccgca gccgcggatc
     5161 gagtaccgag ccgccggcgc tgaacgaata ggccgtcgag ccggtcgggg tggccaccac
     5221 cacgccgtcg gagacccagg tggccaggtg gctaccgctc acctcgacct cgacctggat
     5281 catgcgcacc cttgggcctc gcgccacgac cacctcgttc aggcaggcgt gccgctcacg
     5341 ctcgccgttc gcccgctgca gcgtggcctc gatccggaac cgatcctcga gcgcgaagtc
     5401 gccgctcgcc accttggcca gcgcctgctc caggtcgtcg gtctctacct tggcgaggaa
     5461 cccgacgcgc cccaggttga cccccagggt cggcgccgcg gagtcgccga tcccgcgcgc
     5521 cgcgcggagg aacgtgccgt caccgccgag cacgcagacg aggtcgcttc ccgtgcattc
     5581 ggccgtgatc cggtcgccat catcggcctc cgactcccag gcgtcgactc cgttggcagc
     5641 gcaccaggct cgtgcacgct cgagcgtggc gcgggcctgc ggattggtcg ggttgaacgc
     5701 aaaaccgaag cggctcactc gctcgtccgc agcctcgtgg cgatctcgtc gatccccttg
     5761 cgcagggccg ccagctcggc agtcagctcg tccggggtga cggcggcgta ggaccgatct
     5821 aggcccgccg cgatcaaggc gcgcatcagg ctggagggtg acaccccgcg tccacgggcc
     5881 acctcttcga cccgttccag gagggcgtcc ggcattcgca gcgagacgat ctggcgcatg
     5941 gtgtcgggcg ccggctcgtg gatcggctcg ggcagggcag ccagctcctc gaagacgctc
     6001 agctgttcgg cagccgcagc cggcttgcgc ttcatgccgg ccatcctagc gcgctcgtca
     6061 tacgggcgag ggcgccaccc tgtccacctc cgtcgaccat tgcgccgccg gtccgtctcc
     6121 aggccggagc agggccagga actctcgatt gccgtcggcg ccgacgatcg gcgaggcgat
     6181 cacagcggtc ggcgagaaac cggcccccga ggcgtccgcg ccaaattgca gcagcacgtc
     6241 acggtgggtg gcagggtccc gcaccacgcc gccgcgcggg atggcgtccc ggccggcctc
     6301 gaactgtggc ttgaccaggg cgatcacctg tccctcgcct cgcagcagcg cgcccacggc
     6361 aggaaggacg cggctgagcg atgtgaagga gagatcgagc gtggcgaggt cgatcggctc
     6421 cggcaggtgg cgcagcgctc gcaggttggt gcgctccatg ctgacc
//
LOCUS       DATCQR010000074        10190 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_50386, whole genome shotgun sequence.
ACCESSION   DATCQR010000074 DATCQR010000000
VERSION     DATCQR010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 10190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10190
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50386"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1334
                     /locus_tag="VIN32_02555"
     CDS             <1..1334
                     /locus_tag="VIN32_02555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921490.1"
                     /translation="RYDQSETLLRRAIELHRRRGDRIAAARATAALAQALLRGERVEA
                     ALALLVPASAEFADLSPDPVVVALDGWLARAYASNEEHRSAIEVAERVLDAAEHRDLV
                     EILAATLVTKGFALGSLGRVREGIGVIRAGEELARDNELQAIRLYALVMRGLFQGDID
                     VAAALDGFREGLALARRVGDRSSMLRFINNVGYTAFLAGDWDVALAELDTGLAEENLE
                     RAHRIDLLSNALIVHACRGEPVADGLAELELAIAGDTESNRIAPVEDAKANAALAEGR
                     LDDARVAWRRNAELIAWLAPSSFYQAARPALWDRDVAAVQADLAALDATGFHGRVVEV
                     RRTTIRAGLAALEGRTADALALYREALRGWQDTGLPWDQALTGIDMATLLDPSNPEVR
                     AAAQSARETLVRLGAKPFLARLDAAIGRRTSAESATTAVGESIESRSPTPP"
     gene            complement(1387..2238)
                     /locus_tag="VIN32_02560"
     CDS             complement(1387..2238)
                     /locus_tag="VIN32_02560"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEY8921491.1"
                     /translation="MSGLGLAWSEPFSNVSGLARRMRLRGISRDWAWGGSTGAGVRVG
                     IVDSGLEADHPRLAGRVAQSVAVELVDGDPRIVPDESGDLYGHATACGGIILGLAPDV
                     ELISIRVLGADLRGKGTAFAAGLEWAIDQGIDVLNLSLSSKSESLFPVFHDLVDRAYF
                     KGTVLISAANNVPGASYPSLFSSVFSVAAHSEPDPWRLYYNPAPPVEFGAWGVDVPIA
                     WKDGGSTVATGNSFAAPHVAAMVALILAKHPGLTPFEVKAILAAVADNPRRARAVADA
                     GLGEAVG"
     gene            complement(2235..3011)
                     /locus_tag="VIN32_02565"
     CDS             complement(2235..3011)
                     /locus_tag="VIN32_02565"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEY8921492.1"
                     /translation="MVVDDDLTAALTALRAVGRRSSVARGLQGDVEQRLLQSIVDATA
                     SLFDAEASSIALFEPDPDRLEFRVAAGAHGAGAVGLQVKPTEGIAGFVFSTGQALALT
                     DVASDPRFDLEAAERTGYVPRSVAAVPLLDDEGSVGVLQVLDKRSSPSFSLRDMELLA
                     IFASQATTAITATRVQRDTGRLLGEVLRQIADGTMTDAHVEALVSDVTRGLDDHPESP
                     FWRLVDHVALLRGLGERDLTLVGDILEVVARHAQRARRRA"
     gene            complement(3012..3308)
                     /locus_tag="VIN32_02570"
     CDS             complement(3012..3308)
                     /locus_tag="VIN32_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921493.1"
                     /translation="MGAINQENERFTVMSDQNDPKDTEADRASKPATGEDTEGHRASV
                     PGRSVDEDTEGHRASVPGRSVDQDDTEGHRASVPGRSIDEDDTEGHRASVPGRS"
     gene            complement(3408..4103)
                     /locus_tag="VIN32_02575"
     CDS             complement(3408..4103)
                     /locus_tag="VIN32_02575"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEY8921494.1"
                     /translation="MRDEEFAVHSLEQCALFAHVQPAGLRILAGAMMRRRFRRNEVIF
                     HQGDAGDSLHVVATGAVKIVLPSPDGEEAIIATLRPGDFFGELALLDGAPHSATATAV
                     EPVDTMSLARAPFEQLLDDDRGLRMALLSGLAAELRRLTGQVEELHFLDLAGRLAMRL
                     VRLSRDRDSNANGEVRLDWPFTQSDLAAMIGGTRQSVNKLLSRLMEDGLVLIEHDTLV
                     IHDLDALAERAER"
     gene            4298..4909
                     /locus_tag="VIN32_02580"
     CDS             4298..4909
                     /locus_tag="VIN32_02580"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY8921495.1"
                     /translation="MTRIATGTRSYRSQLRAQQAEETRARILDATGRVMAGGVAFVSM
                     PDVAREAGVSVPTVYRHFATKRDLFGAVYAHAVRIAGLDELVLPRSMDELLGGMRAHF
                     ERTDSLGDLARAAMASPAAQEVRRINMPDRVALFRRLADTIEPKPSKSDRDRIARLLV
                     ILTASSSLRLWRDQLGSSVDEAADDVDWVLRAVIASATSRKGS"
     gene            4906..7104
                     /locus_tag="VIN32_02585"
     CDS             4906..7104
                     /locus_tag="VIN32_02585"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HEY8921496.1"
                     /translation="MNATTIRSATIRRLADVRMADVAEVGGKAANLGELLAAGVRVPD
                     GAVLTAGAADMSADERRSLLNEALPDLGAGPFAVRSSGISEDGSERSFAGMYESVLNV
                     AADEVVAATEQTLASAQTLRAAEYQPGTNGRMAVIIQRMVAPAAAGVVLTADPINGDR
                     QSAVVTAVRGTGDRLVSGAAVGDEWTVQGTVATPRRQPERAIDRRQAAAVASEARRIA
                     VGRGTPQDIEWAIDADGILWIVQARPMTALPPDVSWESTAPGAYTRMLRFGEWIGEPV
                     TRLFESWLLTAMEDRMHAFFREEIGQIAPRPYHVVVNGWYFYSINFISGGAFRRSLPG
                     MLVKAIRSPRHIAGIIPPTVRFSFPIFERRWREDLQPRYRAAVANAERSVETLLVADL
                     PSLIDELAELAGEYFGGIAALSGAAYKMEMNLARFYRLKLAPSLGGSHLPLLAGFDQP
                     ADLKRQGMVSLDWWFAPMPLEATATTPAKNHARVVEAREAAEAAALEALASSPRRLRS
                     FHRLLADAQHLVPIREEQTEELTIAWPVMRRAVLRIGDALAERGMIAEPDDVFFLTRA
                     EALAALDGAPLSPTVDVAGRRSLRTEQARLVPPPLVGRVNPMIQRVWGSFPGLIGARP
                     SDQALVSGVPASPGRATGLVRVIRGPDEFDQLQAGEILVAPLTAPAWTPLFTRAAAVV
                     TDVGSAAAHASIIAREYGIPAVVGCGDATARLQTGMRVTVDGSSGNVEPA"
     gene            7246..8349
                     /gene="cax"
                     /locus_tag="VIN32_02590"
     CDS             7246..8349
                     /gene="cax"
                     /locus_tag="VIN32_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015368 - calcium:monoatomic cation
                     antiporter activity [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/proton exchanger"
                     /protein_id="HEY8921497.1"
                     /translation="MYALTRIPSILGRTTILTVLAAFGAMATAYVLNVLGLSHIVVFV
                     LSAASLAALATLIGHATEQLGHRFGPRATGILQSALGNLPELFISIFALQAGLVIVVQ
                     TALIGSILANTLLVLGLAFLLGGLKNGTQQFHAPAVRSMATLLVLAVAALAIPTLATA
                     PGAPDAGHATDLSVIVSIVLLLIFAASIPFSIGGGVGASADVEAAPPESIWPLGLTVA
                     VLLLGAGGAALTSDLFVEALRPTMASLRISEAFVGLVIVAIAGNAVENVVGVQMAIRN
                     KSDLAASIILNSSLQVAIALTPALVLVSLFVGAGTLTLVLSPLLLGAVALSAILAALI
                     VFDGESTWLEGLALLGLYVIIAASVWFGPPIQV"
     gene            complement(8356..9570)
                     /locus_tag="VIN32_02595"
     CDS             complement(8356..9570)
                     /locus_tag="VIN32_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8921498.1"
                     /translation="MTSDGRLLFATRVARMFGYGLLSVVLVLYLVALGVDGFMVGLIL
                     TLTLLGDAAISLWLTTHADRLGRRRILTAGAGLMLLAGIVFAIAHDPWILLVAATIGV
                     ISPSGNEVGPFLAVEQASLSQTLPDRDRTRIFGWYNLVGSLATASGALVAGLVAQTLQ
                     GSGASEIDSYRAIVIGYAFVGIVLAALFWRLSPAVEVPAAEKTSVATRLGLHRSRGIV
                     FRLAALFSLDAFAGGFVMQSLIAYWFQQRFGADPAALGAIFFGANILAGFSALAAARL
                     AARIGLINTMVFTHLPSNVLLILVPLMPTLPLAVGVLLARFAISQMDVPTRQSYTIAV
                     VDPDERSAAAGVTGIARSLGAAVSPSLATPLLQSVPLAGLPFIIGGGLKILYDLLVLR
                     AFSAVRPPEERS"
     gene            9684..>10190
                     /locus_tag="VIN32_02600"
     CDS             9684..>10190
                     /locus_tag="VIN32_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008083531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEY8921499.1"
                     /translation="MTIDTGNTGFMLLCSSLVMLMTPGLAFFYGGLVSRKNVLTIMMQ
                     SFVSMGWTTIIWFAVGYSLTFSGDVGGVIGNLDMAFLRGVTLDTPSPTPGIPLIVHVA
                     YQMMFAVITPALITGAFANRITFKAYLAFLTLWLLLVYFPFAHMIWGGGILQKWGVLD
                     FAGGIVVHN"
BASE COUNT         1645 a         3439 c         3462 g         1644 t
ORIGIN      
        1 agcgctacga ccaatccgag accctcctgc ggcgggcgat cgagctccat cggcggcgcg
       61 gcgaccgcat cgccgctgcc cgcgccacgg cggccctggc ccaagcattg ttgcgcggcg
      121 aacgggtgga ggcggcgctg gcgctgctgg tgccggcatc ggccgagttc gcggacctct
      181 ccccggatcc ggtggtcgtc gccctcgacg gttggctcgc ccgagcctac gcgtcgaacg
      241 aagagcatcg cagcgcgatc gaggtcgcgg agcgggtgct cgatgcggct gaacaccgcg
      301 atttggttga gatcctcgct gctaccttgg tgaccaaagg gtttgcgctg gggagtctgg
      361 ggcgggttcg cgaagggatc ggggtcatcc gagcggggga agagctcgcc cgcgacaacg
      421 agttgcaagc catccggctc tatgccctcg tcatgcgggg ccttttccag ggcgacatcg
      481 acgtcgcggc cgcactggat gggttccgcg agggtctggc gctcgcccgc cgggtcgggg
      541 accgctcctc gatgctgcgc ttcatcaaca acgtcggcta caccgccttc ctcgcaggag
      601 actgggatgt ggcactcgcc gagcttgaca ccgggctcgc cgaggagaat ctcgagcggg
      661 cccaccgcat cgacctgctc agcaacgcac tcatcgtcca tgcctgtcgc ggcgagccgg
      721 tcgccgacgg tctcgctgag ctcgagctcg cgatcgcagg cgataccgaa tcgaaccgca
      781 tcgcgcctgt cgaggacgca aaggccaacg cggcgctcgc cgaagggcgg ctcgacgacg
      841 cccgcgtcgc gtggcggcga aacgcagagc tcatcgcgtg gttggctcca tcatcgttct
      901 accaggccgc gcggcctgcc ctatgggatc gagatgtcgc ggccgttcag gccgatctcg
      961 cggcactcga tgcgaccggc ttccacggcc gggtggtgga ggtccgcaga acgacgattc
     1021 gagcggggct ggccgccctg gaaggtcgca ccgcggatgc cctggcgtta taccgcgagg
     1081 cgttgcgagg ttggcaagac acggggctcc cctgggacca ggcgctgacc gggatcgaca
     1141 tggcgaccct gctcgaccca tctaatccgg aggtccgcgc cgctgcacag tcagctcgag
     1201 aaaccctcgt tcggctgggc gcgaagccgt tcctggcgcg actggacgcg gcaataggcc
     1261 gtcggacctc tgccgaatcg gccaccaccg cggtcggaga atcgatcgaa agccggtccc
     1321 cgacgccccc gtgacgacca gctcctcgac gcgaagctcg atcttctgag cgcggtgcta
     1381 caacgctcag ccgactgctt ccccgaggcc ggcatcggca accgcgcggg cacgtcgtgg
     1441 gttgtcggca accgccgcca ggattgcctt cacctcgaag ggcgtcagcc caggatgctt
     1501 cgcgaggatg agcgccacca tcgccgcaac gtgtggtgcg gcgaagctgt tcccggtggc
     1561 gaccgttgaa ccgccatcct tccaggcgat cggaacatcg actccccagg caccgaactc
     1621 cactggcggc gccggattgt agtagaggcg ccacgggtcg ggttcgctat gagcggctac
     1681 cgagaagacg gaggagaaga gcgatggata gctcgcccca ggcacattgt tggcggcgct
     1741 gataaggacc gtgcccttga agtaggcacg gtccaccagg tcgtgaaaga cgggaaagag
     1801 ggactcgctc ttcgacgaga ggctgaggtt gagcacatcg atgccctggt cgatggccca
     1861 ttccaggccg gcagcgaagg ccgtcccctt cccgcgcaga tcggcgccca ggacccggat
     1921 cgagatgagc tccacatcgg gcgcaagccc gaggatgatc ccgccacagg cagttgcatg
     1981 accgtagagg tccccgctct cgtccgggac aatgcgggga tctccgtcga cgagctcgac
     2041 cgccacgctc tgcgccaccc ggccggcgag acggggatga tcggcctcga ggccgctgtc
     2101 gacgattccc acccgcacgc cagcgcccgt ggagccgccc cacgcccaat ctcgtgagat
     2161 gccccttagc cgcatgcggc gagcaaggcc cgaaacattg ctgaagggct ccgaccacgc
     2221 caggccgaga ccactcacgc tcgtcgacgc gctcgttgcg cgtgtcgcgc caccacttcg
     2281 aggatatcgc ccacgagagt gagatcgcgc tcacccaggc cgcgcagcaa cgcaacgtgg
     2341 tccaccaacc gccagaaagg ggactccgga tggtcgtcga gcccgcgcgt cacgtctgac
     2401 accagggcct cgacatgcgc atcggtcatc gttccgtccg cgatctgccg aaggacctcg
     2461 cccagcaggc gcccggtgtc gcgctgcacg cgcgtggcag ttatggcggt cgtcgcctgg
     2521 ctggcgaaga tggcgagcag ctccatgtcc cgcaacgaga agctcggcga gctgcgcttg
     2581 tcgaggacct ggagcacgcc caccgatccc tcgtcgtcga ggagcgggac cgcagcgacc
     2641 gaacgaggta cgtagccggt gcgctcggca gcctcgaggt cgaaacgcgg atcggaggcg
     2701 acatcggtca gcgcgagcgc ctgccctgtt gaaaagacga accccgcaat gccctcggta
     2761 ggctttacct ggaggccgac cgcgccggcg ccgtgggccc cggcggcgac gcgaaattcg
     2821 agccgatccg ggtcgggctc gaagagagcg atcgaggacg cctctgcgtc gaagagactt
     2881 gccgtggcgt ctacgatgga ttgcaggaga cgttgctcaa cgtctccctg gagtccgcgg
     2941 gcaacggaag accgccgccc caccgccctg agggcggtga gtgcggcggt cagatcgtca
     3001 tcgacgacca ctcagctgcg gccaggaacc gaagcccggt ggccctcggt gtcgtcctcg
     3061 tcgatgctgc ggccgggaac agaggcccgg tggccttcgg tgtcgtcctg gtcaacgctc
     3121 cggccaggaa ccgaggcgcg atggccctcg gtgtcctcgt cgacgctccg gccaggaacg
     3181 gaggcgcgat ggccctcggt gtcctcgccg gtggccggct tggatgccct atcggcctcg
     3241 gtgtccttgg gatcgttctg gtctgacatt acggtgaacc tctcattctc ttggttgatc
     3301 gcgcccatct tacttgcgag cgccgttgat ccgaacgata agtcccatct tcgcgggtga
     3361 gaagcccctt cccgacttgg ttcgtgtcga attgccgaca accaagctca tcgttcagca
     3421 cgttccgcga gggcatcgag atcgtggatg acaagcgtgt cgtgctcgat gagcaccagt
     3481 ccgtcctcca tgaggcggct gagcagcttg ttcacactct gccgagtgcc accgatcatg
     3541 gcggcgaggt cggactgggt gaacggccag tcgaggcgca cctcgccgtt ggcgttggaa
     3601 tcgcgatctc gggacaggcg cacgaggcgc atggccagcc gtcccgcgag gtccaggaaa
     3661 tgaagctcct ccacctgccc ggtcaggcgg cggagctccg cagccaagcc ggacaggagc
     3721 gccattcgga gtccgcgatc atcgtcgagc agctgctcga atggtgcgcg ggcgaggctc
     3781 atggtgtcga ccggctcgac ggcagtggcc gtggcggaat gcggcgcgcc atcgaggagt
     3841 gcaagctcgc cgaagaagtc gcccggccga agagtggcga tgatcgcttc ctcaccatcc
     3901 ggcgagggca ggacgatctt cacggccccg gtcgccacca cgtggagcga gtcgcctgca
     3961 tcgccctggt ggaagatgac ctcgttacgc ctgaatcggc gccgcatcat ggctcccgcc
     4021 aggatgcgca ggcccgcggg ctgcacgtgg gcaaagagag cgcactgctc caggctgtgg
     4081 accgcgaatt cctcgtcccg cacttcaacc ccaaaactgg accattggct ggcacgagcc
     4141 ccatgccgcg cccggtcgcg ccatgttagt ccgtgagcct gcgccagtgc ccagatacga
     4201 agggcggcgt ccgtcgtggc gacactcttc acgcgagcca cgaaggccgt cgtcccctcc
     4261 ctccatcggc tgttgacacg agtcctatat tcacaatatg actcgtatcg ccaccgggac
     4321 gcgctcctac cgcagccaac tccgagccca gcaggccgaa gagacccggg cgcgcatcct
     4381 cgatgccacc ggccgggtca tggccggcgg cgtcgcattc gtgtccatgc ccgatgttgc
     4441 gcgcgaggcg ggcgtgtcag tgccgaccgt ctaccgccac ttcgcgacga agcgcgacct
     4501 ctttggggcg gtgtacgcac acgccgtgcg cattgcaggc ctcgacgaac tcgtcctgcc
     4561 acgctcgatg gatgagctcc tgggaggcat gcgagcgcat ttcgaacgca ccgactcgct
     4621 cggcgacctc gcccgggctg ccatggccag tccggcggcc caggaagtga ggcggatcaa
     4681 catgccggat cgggtcgcgc tgttccgccg cctcgcggac acgatcgagc cgaagccatc
     4741 caaatcggat cgcgatcgga tcgcacgcct gctggtgatc ctgaccgcgt catcgtccct
     4801 gcgcctgtgg cgcgatcaac tcggctcatc ggtcgacgag gcggccgatg acgtggactg
     4861 ggtcctgcgc gccgtcatcg cctcggccac ttcgaggaag ggatcatgaa cgcgacaaca
     4921 atccggtcgg caacaatccg acggctcgcc gacgtgcgca tggccgatgt cgccgaggtt
     4981 gggggcaagg cagccaacct gggcgagctc ctggcggcgg gcgtccgcgt ccccgatggc
     5041 gccgtcctga ccgccggggc cgccgacatg tcggccgacg agcgccgatc cctgctgaac
     5101 gaggctctgc ctgacctggg cgccggcccc tttgctgtcc gatcgagcgg gatctccgag
     5161 gatggcagcg agcgctcgtt tgccggcatg tacgaatcgg tgctgaatgt cgccgcagat
     5221 gaggtcgtgg cggcgaccga acagaccctt gccagtgctc agaccttgcg tgctgccgaa
     5281 taccagcccg gcaccaacgg gcgcatggcc gtgatcatcc agcggatggt cgcgccggcc
     5341 gcggccggcg tggtcctcac tgccgatccg atcaacgggg atcggcaatc cgctgtcgtt
     5401 accgcggtgc ggggcaccgg ggatcggctg gtgtccgggg ccgccgttgg tgacgagtgg
     5461 accgtgcaag gaaccgtcgc cacacctcgc cgtcagcccg agcgcgcgat tgatcgtcgc
     5521 caggcggcgg ccgtcgcgag cgaggcgcgg cgcatcgcgg ttgggcgcgg gaccccgcag
     5581 gacatcgaat gggcgatcga cgcggacgga atcctttgga tcgtccaggc ccggccgatg
     5641 acggcgctcc cgccggatgt gtcgtgggaa tcaaccgcgc ccggggcgta cacgcggatg
     5701 ctccgattcg gcgagtggat cggcgaaccg gtcactcgcc tcttcgagtc ctggctgctg
     5761 acggccatgg aggaccgcat gcacgcgttc ttccgcgagg agatcggcca gatcgcaccg
     5821 cggccgtacc acgtcgtggt caacggttgg tatttctact cgatcaactt catctctgga
     5881 ggcgccttcc ggcgcagcct gcccggcatg ctggtgaagg ccattcgctc gcctcgccac
     5941 atcgcaggga tcattccgcc gaccgttcgc ttcagctttc cgatcttcga gcggaggtgg
     6001 cgcgaggacc tgcagccgcg ctaccgagcg gctgtcgcca atgccgaacg cagcgtcgag
     6061 acactgctcg tcgccgacct gccgtcactg atcgatgagc tggccgagct cgccggcgag
     6121 tacttcggtg ggatcgcggc gttgagcggt gccgcataca agatggagat gaacctcgcg
     6181 cggttctatc ggctcaagct ggctccgtca ttgggcggca gtcacctgcc gctcctggca
     6241 gggttcgatc agccggccga tctgaagcga cagggcatgg tgtcgctcga ttggtggttt
     6301 gctccgatgc cgctcgaggc gacggcaacc accccggcca agaatcacgc ccgcgtcgtg
     6361 gaggcgaggg aggcggccga ggccgccgcg ttggaggctc tggcgtcgtc gccacgccga
     6421 ctccggtcct tccaccgcct gctggcagac gcgcagcacc tcgtcccgat tcgggaagag
     6481 cagacggagg agctgacgat cgcctggccg gtgatgcgac gtgcggttct gcggatcggc
     6541 gatgcgctgg cggagcgcgg catgattgcc gagcccgatg acgtcttctt ccttacgcgg
     6601 gccgaggccc tcgcagcgct cgacggggcg ccgctgtcac cgaccgtcga cgtggccggc
     6661 cgacgatcac tgcggacgga gcaggcccgc ctggtgccgc cgccgctcgt gggccgggtg
     6721 aacccgatga tccagcgggt atggggcagc ttcccaggac taatcggtgc ccgtccatcg
     6781 gaccaggccc tggtatccgg cgtaccggcg tctccgggtc gcgcgaccgg gctggtgcgg
     6841 gtgatccgcg ggcctgacga gttcgaccag ttgcaggccg gcgagatcct cgtggcgccg
     6901 ctcaccgcac cggcgtggac gccgctcttc acacgtgcgg ccgcggtcgt caccgacgtg
     6961 gggagcgcgg cggcgcacgc ctcgatcatc gctcgtgagt acggaatccc cgcggtcgtt
     7021 ggctgtgggg acgccacggc tcgactgcag accgggatgc gcgtcacggt cgacggcagc
     7081 agcggaaacg tggagccagc gtgaggccac gtcccggcat cggtcagggc tgaagatcgg
     7141 tcgcgacgta ccggccttcc aggtcgggcg accaatgacc tgggccagcg gccctcgtct
     7201 agtgggcctc ggtcgaacga cctgtggcca tacaatcgca ggccgatgta cgcgctgacc
     7261 cgaatcccct cgatccttgg gcgtacgacc atcctgacgg tgctcgcggc gttcggcgcg
     7321 atggcaaccg cgtacgtcct caatgtgctc ggcctcagcc acatcgtcgt cttcgtcctg
     7381 agcgcggcgt cgctggccgc cctggccacc ttgatcggcc acgctacgga acagctgggc
     7441 catcggttcg gacccagggc caccggaatc cttcagtcgg ctctcggcaa tctgcccgag
     7501 ctcttcatct cgatcttcgc cctgcaggcc ggtctggtga tcgtggtgca gaccgccctc
     7561 atcggctcca tcctggccaa caccctcctg gtgcttggcc tcgccttcct gctcggcggc
     7621 ctcaagaacg ggacgcagca gttccacgcg ccggcggttc gcagcatggc gacgctgctg
     7681 gtgctggctg tcgccgccct ggcgatcccc acgctggcca ccgctcccgg ggccccggac
     7741 gccggtcatg caacggacct gagcgtgatc gtgtcgatcg tactgctcct gatcttcgcc
     7801 gcttcgatcc ccttctccat cggtggcggg gtcggcgcca gcgcggatgt cgaggcggcc
     7861 ccgccagagt cgatctggcc gttggggctg acggtggcag tcctgctcct gggcgcgggt
     7921 ggtgcggccc tcacctcgga cctgttcgtc gaggcgctgc ggccgacgat ggcaagcctg
     7981 cgcatctcgg aggcgtttgt cggattggtg atcgtggcga tcgccgggaa tgccgtcgag
     8041 aacgtcgttg gcgtgcagat ggcgatccgc aacaagtcgg acctcgcggc gagcatcatc
     8101 ctgaacagca gcctgcaggt cgcgatcgcc ctgacgcctg ccctcgtcct ggtctccctg
     8161 ttcgtggggg caggcacgct caccctggtg ctgagcccgc tcctgttggg ggctgttgcg
     8221 ctctcggcca tcctggcggc gctcatcgtg ttcgacggtg agagcacatg gctcgagggg
     8281 ctggcgctgc tcggcctcta cgtgatcatc gccgccagcg tctggttcgg gccgccgatc
     8341 caggtctgac ggcggtcagc tgcgctcctc cggcggtcgc acggcactga aggcgcgcag
     8401 caccagcagg tcgtagagga tcttcaggcc tccgccgatg atgaacggca gccctgccag
     8461 cggcacgctc tggagcaggg gggtggccag cgagggcgag accgccgcgc ccaggctgcg
     8521 agcgatgccg gtgacaccgg ccgcggccga tcgctcatcg gggtccacca ccgcgatggt
     8581 gtaggactgg cgggtcggca cgtccatctg gctgatggcg aagcgggcca gcaggacgcc
     8641 cacggccagc ggcagggttg gcatcagcgg cacgaggatc aggagaacgt tcgagggcag
     8701 gtgggtgaag accatggtgt tgatgaggcc gatgcgcgcg gccagccgcg cggcagccag
     8761 cgccgagaac cccgccagga tgttggcccc aaagaagatg gcgcccagcg cggcagggtc
     8821 ggcgccgaag cgctgctgga accagtaggc gatcaggctc tgcatcacga agccgccggc
     8881 gaatgcatcc agtgagaaga gggcggcgag ccggaagacg atcccgcgcg accgatgcag
     8941 gcccagccgg gtggcgaccg acgtcttctc cgcggcaggg acctccaccg cgggtgacaa
     9001 ccgccagaag agggcggcca ggacgatccc gacgaacgcg tagccgatca cgatcgcccg
     9061 ataggagtcg atctcggatg ccccagagcc ctggagcgtc tgcgcgacga gcccggccac
     9121 gagggccccg ctggccgtcg ccagcgagcc gaccaggttg taccagccga agatccgcgt
     9181 ccggtcgcgg tcaggcaggg tctgggagag ggaggcctgc tcgacggcaa ggaacggccc
     9241 cacctcgttt ccgctggggc tgatgacccc gatggtggcc gccaccagca ggatccaggg
     9301 atcgtgggcg atggcgaaga cgattccggc cagcagcatg agccccgcgc cggcggtcag
     9361 gatccggcgc cgcccgaggc gatcggcatg cgtcgtcagc cacaggctga tcgccgcgtc
     9421 gccgagcagg gtgagtgtca ggatcaaccc gaccatgaag ccgtcgacgc caagcgccac
     9481 caggtagagg accagcacca ccgagagcag gccgtacccg aacatgcggg cgacgcgcgt
     9541 ggcgaagagg aggcgcccgt cggaggtcac cggcggatgc taatggctta cgaggtcgag
     9601 ggggttctcc atcggtttca gccattgacg ccagcacagc gcgctcccca gaatgcggct
     9661 gcccaatccc ggagagacga ccgatgacca tcgacactgg caataccggc ttcatgctgc
     9721 tgtgcagcag cctggtgatg ctgatgaccc cggggcttgc gttcttctac gggggcctgg
     9781 tcagccgcaa gaacgtgctg acgatcatga tgcagtcctt cgtatcgatg ggctggacga
     9841 ccatcatctg gttcgcggtt ggatattcgc tcaccttctc cggcgacgtc ggcggagtca
     9901 tcggcaacct ggacatggcc ttcctgcgtg gcgtcacgct cgacacgcca tcaccaactc
     9961 cggggatccc cctcatcgtc cacgtcgcct accagatgat gttcgcggtg atcacccccg
    10021 cactgatcac gggtgcgttc gccaaccgaa tcacgttcaa ggcctacctt gccttcctaa
    10081 ccctctggct gctgctggtc tacttcccgt tcgcccacat gatctggggc ggcggcatcc
    10141 tgcagaagtg gggggtgctc gacttcgccg gcggcatcgt ggtacacaac
//
LOCUS       DATCQR010000075         3358 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_220590, whole genome shotgun sequence.
ACCESSION   DATCQR010000075 DATCQR010000000
VERSION     DATCQR010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3358
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220590"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1937)
                     /locus_tag="VIN32_02605"
     CDS             complement(<1..1937)
                     /locus_tag="VIN32_02605"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEY8921500.1"
                     /translation="MSLITLTGIAKSHGAQHLFDDVNLQIGTGRRIAIVGPNGAGKTT
                     LLEIITGEQEADAGTVTRGKDLAIGYLRQEVAQAQGRTVVAEVVAGAGAVSGIERRMR
                     HIEAEMAEADGEAATKDELAELMDEYGRLQHRFEQLGGWSLEAEARRILAGLGFAQAD
                     MDRDIGQFSGGWMMRVALGRLLLANPDLLLLDEPTNHLDLASVEWLQGFLAEYAGAVV
                     LVSHDRDFINAVVNRVAELNHGVFTEYVGDFADFVDQRDERIAQLEMAAKAQGRTIAR
                     IERYIERFRYKKAKAKQVQSRIKQVARIERIDVPTNRTRAVKFRFPEPRRSGRVVITL
                     DGIVKRYGAHSVYDGLDLVLERGQKVALIGPNGAGKSTLLKILAGVLPFEGGTRELGT
                     NVQVAYFAQHQIDALNPANTVFEELAGAAARMSTAEMRRLLGAFLFTGDAIEKKVEIL
                     SGGEQTRLALAKLMADPANLLCLDEPTNHLDIQSRDVLEDALNAFQGTIVLITHDRYL
                     IRSVANAIIEVNAGEATLYPGDFEYYAAKRGLDIETRGAVEGIATPRGIEAVPSRPRE
                     SSVEAAARKRTEAEARNRRYRRTRDLRDALQRVEQEVRETDASLAEMSERLADPATYA
                     DHHAFRELIDRHNAARDRADQL"
     gene            complement(1934..2872)
                     /gene="thrB"
                     /locus_tag="VIN32_02610"
     CDS             complement(1934..2872)
                     /gene="thrB"
                     /locus_tag="VIN32_02610"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEY8921501.1"
                     /translation="MPTPIATIEVPASSANLGSGFDCFAAALSLRLRGELYAGDEAGI
                     LLNAHGEGAPGPHDDPEQNLLIRAFREGLRLAHGSQHGAGAWRIEVSSMIPAARGLGS
                     SAAAIVAGLLLGAAAGRHAPDADELLTMAATLEGHPDNVAAALYGGVTLSVAGEVGTT
                     PLLRRFRVPEAWIPVLFVASATSRTQGMRDALPAAVPHHEAAAAAARAALLATAVITS
                     DAGLLRTAMEDRLHQPYRLPLLPGSRGLIEEAYEHGAAGAALSGAGPSVLAICDSPIT
                     AHAVEKAWGASGVPGMATRLRFDTDGARLSTPETVR"
     gene            complement(2900..>3358)
                     /locus_tag="VIN32_02615"
     CDS             complement(2900..>3358)
                     /locus_tag="VIN32_02615"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEY8921502.1"
                     /translation="LLCLEGAQAGLAWITILRKRDGYRRAFEGWDPERIAAYEEKDEA
                     RLLADPEIVRNRAKVRSVIGNANAYLRLADTPGAFDHYLWAFVDVTPVVNRWTSLSGV
                     PAETDASRALSRDLKKRGFSFVGPTIVYAFMQSAGLVNDHLVSCFRHDEV"
BASE COUNT          472 a         1159 c         1175 g          552 t
ORIGIN      
        1 agctggtcgg ctcggtcacg ggccgcgttg tgacgatcga tcagctcgcg gaatgcgtgg
       61 tgatcggcgt aggtggcagg gtctgccagg cgctcgctca tctcggccag ggaggcatcg
      121 gtctctcgga cctcctgctc gactcgttgc agggcgtcgc gcaggtctcg cgtccggcgg
      181 tagcgccgat tgcgcgcctc cgcctccgtt cgcttgcgtg cggccgcctc gaccgacgat
      241 tcgcgcggac gcgacggcac ggcctcgatc ccgcggggag tggcgatccc ctccaccgcc
      301 ccgcgcgtct cgatgtccag cccgcgcttg gcggcgtagt actcgaagtc gccggggtag
      361 agggtcgcct cgccggcgtt gacctcgatg atggcgttcg ccaccgagcg gatcaggtat
      421 cggtcgtggg tgatcagcac gatcgtcccc tggaaggcgt tgagggcgtc ctccagcacg
      481 tcgcgagact ggatgtccag gtggttggtc ggctcgtcga ggcacaggag gttggccggg
      541 tcggccatca gcttggcaag cgccagccgc gtctgctcgc cacccgacag gatctcgacc
      601 ttcttctcga tcgcatcccc ggtgaagagg aacgcgccaa gcaggcggcg catctcggcg
      661 gtgctcatcc gggcggcggc cccggccagc tcctcgaaga ccgtgttggc cgggttcagg
      721 gcgtcgatct ggtgctgggc gaagtaggcg acctgcacat tggtgcccag ctcacgggtg
      781 ccgccctcga agggcaggac gccggccagg atcttcagca gcgtgctctt tccggctccg
      841 tttggaccga tgagtgccac cttctggccg cgctccagga ccaggtcgag cccgtcgtag
      901 acgctgtgcg ccccgtagcg cttcacgatc ccgtcgaggg tgatgacgac gcggccgctg
      961 cgccgtggct cggggaagcg gaacttgacg gccctggtcc gattcgtggg gacatcgatc
     1021 cgctcgatgc gcgcgacctg cttgatccgg ctctggacct gcttggcctt cgccttcttg
     1081 tagcggaagc gctcgatgta gcgctcgatg cgggcgatgg tccggccctg cgccttggcg
     1141 gccatttcga gctgggcgat acgctcgtcg cgctggtcga cgaagtcggc gaaatcgcca
     1201 acgtattcgg tgaacacgcc gtggttcagc tccgcaacgc gattgacgac ggcgttgatg
     1261 aagtcgcggt cgtggctgac cagcacaacc gccccggcgt actcggccag gaacccctgc
     1321 agccactcga ccgacgccag gtcgaggtgg ttggtcggct cgtcgaggag gagcaggtcg
     1381 ggattggcga gcaggaggcg accgagcgcc acccgcatca tccagccacc tgagaattgg
     1441 ccgatgtcac ggtccatgtc cgcctgggcg aagccgagac ctgccaggat gcgtcgcgcc
     1501 tccgcctcca gcgaccagcc gccgagctgc tcgaagcggt gctggagccg gccgtactcg
     1561 tccatcagct cggccagctc gtccttggtg gccgcctcgc cgtcggcctc ggccatctct
     1621 gcctcgatgt gccgcatccg gcgctcgatg ccgctcaccg ccccggcgcc ggccaccacc
     1681 tcggccacca ccgtgcggcc ctgcgcctgg gccacctcct gacgcaggta gccgattgcc
     1741 aggtccttgc cccgggtgac ggtcccggcg tccgcctcct gctcgccggt gatgatctcg
     1801 aggagggtcg tcttgcctgc gccgttaggc cccacgatgg cgatccgtcg cccggtcccg
     1861 atctgcaggt tgacgtcgtc aaagaggtgc tgggcgccgt ggctcttggc gatcccggtc
     1921 agcgtgatga ggctcatcgg accgtttcag gcgtgctgag gcgtgctcca tcggtgtcga
     1981 agcgaagccg ggtcgccatg cccggtacgc cggaggcacc ccaggccttc tcgactgcgt
     2041 gggcggtgat cggtgaatcg cagatggcca gcacgctggg gccggcgcct gaaagcgccg
     2101 caccggcagc cccatgctcg tacgcctcct cgatcagccc tcgcgatccc ggcaggaggg
     2161 ggaggcgata cggctggtgc aggcgatcct ccatcgccgt ccgcagcagc cccgcgtccg
     2221 aggtgatgac agccgtcgcc agcagggcgg cccgcgcggc tgctgcggca gcctcgtggt
     2281 gcgggacggc tgcagggagc gcatcgcgca tcccctgcgt ccggctggtg gcagaggcga
     2341 cgaagaggac cgggatccac gcctctggca cgcggaacct tcgcaggagg ggtgttgtgc
     2401 ccacctcgcc ggccaccgac agggtgacgc caccgtacag ggcggccgca acgttgtccg
     2461 ggtggccctc cagcgtcgcc gccatggtga ggagctcgtc ggcgtcgggg gcgtgccgcc
     2521 cggccgccgc tccgagcaag agacccgcca cgatggcagc cgcgctgctc cccagcccac
     2581 gcgcggccgg gatcatgctg ctcacctcga tccgccaggc gccggcaccg tgctgcgacc
     2641 cgtgcgccag gcgcagcccc tcgcggaacg cccggatcag gaggttctgc tccgggtcgt
     2701 cgtgtggccc cggggctcct tcgccgtggg cgttcagcag gatgccggcc tcgtcaccgg
     2761 catacagctc gccgcgcagc ctcaggctga gcgcggcggc gaaacagtcg aagccacttc
     2821 ccaggttggc gctgctggcc ggcacctcga tcgtggcgat gggcgtgggc atgcggcccg
     2881 agtatacgga cggtggatgc tagacctcgt cgtggcggaa gcagctgacc aggtggtcgt
     2941 tgaccaggcc agcggactgc atgaaggcat acacgatggt cggcccaacg aacgagaagc
     3001 cgcgcttctt caggtcgcgc gacagcgcgc gcgaggcatc ggtctcggcg ggcacgccgc
     3061 tgagcgaggt ccatcggttc acgacgggcg tcacgtccac gaacgcccac aggtagtggt
     3121 cgaaggcacc gggcgtgtcg gccaagcgca ggtaggcatt cgcattgccg atgacggacc
     3181 tgaccttggc ccgattgcgc acgatctcgg ggtcggccag gaggcgagcc tcgtccttct
     3241 cctcgtaggc ggcgattcgc tcgggatccc agccctcgaa ggcacgccgg tagccgtcgc
     3301 gctttcgcag gatcgtgatc caggccaggc cagcctgcgc tccctccagg cacagcag
//
LOCUS       DATCQR010000076         8552 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_128130, whole genome shotgun sequence.
ACCESSION   DATCQR010000076 DATCQR010000000
VERSION     DATCQR010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 8552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8552
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128130"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1229)
                     /locus_tag="VIN32_02620"
     CDS             complement(<1..1229)
                     /locus_tag="VIN32_02620"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEY8921503.1"
                     /translation="MTNVAEATPVSGDVQPPARTSLHSLLAQTEIDLRLFGMLVALAL
                     ILLTFNILSGGKFFQPTNMITLAVQATGVAIIATGMVMIIVSRNIDLSVGSVVGVIAM
                     TYALLMTDWMPNVLGMGPDFPFRWVIALGIGVGLGAAIGALQGFIIAYIGVPSFIVTL
                     GGLLSIRGVIWYLSSGAAVSGLDSGFQLIGGGALGSIGGTATWILGIVACVPIIALLL
                     TGRRQRRRYGFPVRPVWAEVLLGFISCAAVLGLAAFANANLWPKGLANRYAAEHGIPI
                     PAGGLHISIGFPYPIMLLIGVTLVMTWIATRRRFGRYVYAYGGNPDAAELAGINTRWT
                     ILKTYVLMGVLCALAAAIAAARLNGSTLDVGQGYELYVIAAAVVGGTSFAGGIGTIPG
                     AVLGAFVMQSLAYGLSF"
     gene            complement(1319..2479)
                     /locus_tag="VIN32_02625"
     CDS             complement(1319..2479)
                     /locus_tag="VIN32_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020038546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEY8921504.1"
                     /translation="MSIRRLAALTALAAVILAACTSQSPAASVGGSASAGPSGAPCVV
                     GVSWNNFQQPRWAAHDEPNLKKTVEDGGGSYISADANLSSEQQLTDVDTLISQGANVL
                     VLLAQSNTAILPALQKAKDAGIPVIAYDRLIEDPGVLYITFDNVLVGKAEATAILTKV
                     PKGNYVLIKGDPGDANASTFLPKGWDDAGLKDKVASGDIVLLNGPDGTFTTAWTTTLA
                     QANMEAIIDKAVADGKKIDAILAENDSTALGVVGALTAKSYGFPPLSGQDGDPANLNN
                     VAQGKQYVDVFKNANELGKAAGAAALALCNGTATADLTLPDGLIDPTVAPVAGLSAQE
                     FTTPNGTKVSSFILQPTPLTADTLQTAVDAKQISKADLCKGVDAATAPAACK"
     gene            2803..3510
                     /locus_tag="VIN32_02630"
     CDS             2803..3510
                     /locus_tag="VIN32_02630"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012706183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY8921505.1"
                     /translation="MSPLEGKVALVTGASRGIGAATARTLAGAGAQVAIASRSGENPG
                     VDGALAQACDVRDRAALDAMVAATVARFGRLDILVVNAGVGAYGPFLDLPAADLEEMI
                     DVNVKGALYAVRAALPELLKSEAADIVTIASEAGRRGLPYEVAYCASKFAQVGMTAAL
                     DHELREHGVRCTNVCPGGVATDFAMGHGRTPEMPQLAGMMVPQDVAEAVLFVVTRPRS
                     HRILEIAFRPMTESSWG"
     gene            3507..4520
                     /locus_tag="VIN32_02635"
     CDS             3507..4520
                     /locus_tag="VIN32_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY8921506.1"
                     /translation="MSPLPELRWGILSTASIARQKVIPGIQRADRCRVVAIASRDTAR
                     ANEVAREAGIPTAHSSYEALLADPGVDAVYIPLPNHMHAEWTIAAAQAGKHVMCEKPL
                     ALTSADAERMVEACDAHGVRLMEALMYRQHPSWVAVRELVAAGRIGRLTAVQSWFSYF
                     NDDPADIRNVGAFGGGALFDIGCYCVNLSRMLFDGEPTRVASSIVRDAASGVDVLTSG
                     LLEFTGGGVATFTCTTRAEDDQRVHIYGTQGRISIGIPFNIPPDRPTEVYLTMGGDPP
                     VAPHTETITFETADPYTVEAERFATAVLDGGPTPVSPADAIANLRVIERLLAADPRAT
                     GPS"
     gene            4544..6415
                     /locus_tag="VIN32_02640"
     CDS             4544..6415
                     /locus_tag="VIN32_02640"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF044997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HEY8921507.1"
                     /translation="MQSRQLRIIAALVVSLAIVGGGLLAVLRPWEKQTTCPTVAVHPE
                     WSVARRWDEALLDAIRRSLPNPPVHARNLYHLAAAMWDAWAVYDPVADGVFFREKHTA
                     SDVAAARNEAISYAAYRVLSARFIKAVGGSDSLSEFADVMDALCYPLDVTNTTGDTPA
                     TIGNRIAKTVLAAGLLDGSNQAGAYADPNYEPVNAPLVVGGTGATMIDPNRWQPLQIE
                     HMISQNGIPVTNGVQVAIGTHWGRVTSFALASAGPNGVPIDPGPPPLLGDPATDSELK
                     AQLVELIRDSSELDPANDATIDISPGARGGNTLGSNDGHGHAANPATGQPYASDVVKR
                     ADFGRVMAEFWADGPKSETPPGHWNVIANLVSDELAPNLRLGGTGPRVGRLQWDVKLY
                     LALNGAVHDAAIAAWGLKGHYDSSRPISLIRYMGGLGQSSDPSLSSYDPEGLPLVPGL
                     IELITAGTTAPGERHAALSGHEGAVAVYAWAGNPADPKTEVGGAAWILATRWIPYQLP
                     TFVTPSFPGYASGHSAFSRAAAEVLTAYTGTEYFPGGVSGYTIKAASLKFEKGPTTDI
                     GLEWATYYDAADQAGQSRLWGGIHIQADDFTGRMLGSQCGKDAWAQAERYYTGTAIQ"
     gene            6426..8078
                     /locus_tag="VIN32_02645"
     CDS             6426..8078
                     /locus_tag="VIN32_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008194522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HEY8921508.1"
                     /translation="MTRGRIGLLVALLAFAAIAGAAVLLLRTPTPTDAAGPPQFIDET
                     AASGVSQRFDGPETYQVGGGVAVFDCNGDGLPDLYLGGGEGPASLFRNRSTVGGALRF
                     DRVASSITDLPMVNGAYPLDVDGDGEVDLAILRIGENVLLRGHGDCTFERANEMLGFS
                     GVDGYTTAFSATWEGDASLPTLAIGHYLKVDSHGDTTFTCDEGDLLRPNPGGSGYGTA
                     APLAPGYCSLSMLFSSWDGSSRRDLRVSNDRQYYTDGSEQLWRMTPGTTPGLYSADDG
                     WASLQIYGMGIASQDLTGDGLPEVYLTSQQDNKLQTLTAGSSQPAFRDIALRRGVTST
                     RPFAGDTSLPSTAWHPEFADVNNDGFMDLFVSKGNVGAQADYAMRDPSDLFLGQPDGT
                     FVQAADRAGILRFERGRGAALADLNLDGLLDLVEVNYGADTVVWRNVGDGTADAPSAM
                     GHWLMLDIRQPGPNRDAIGAWVDVRIGDVTVRHELTIGGGHLSGELGWLHVGLGPATS
                     AEVRVLWPDGEQGPWLHAQADSPAIVERGASAVQPWSPAGGG"
     gene            8178..>8552
                     /locus_tag="VIN32_02650"
     CDS             8178..>8552
                     /locus_tag="VIN32_02650"
                     /inference="COORDINATES: protein motif:HMM:NF013431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921509.1"
                     /translation="MGARLHRLRAFGHDLLRTPDDPTEHVRDPFRWGAYLMAPWCNRI
                     AAEPVDINGKTVSLPSNFPDGTAIHGQVYDAPWDVQSDDSLEIIAGGDGWPWRYRCSL
                     RVTIADAALTIEQSLTNLADSPM"
BASE COUNT         1382 a         2881 c         2895 g         1394 t
ORIGIN      
        1 atgaagctca atccgtaggc gagcgactgc atgacgaagg cgcccagcac ggcgcccggg
       61 atggtcccga tcccgccagc aaacgacgtg cccccgacga ctgcggcggc gatcacgtac
      121 agctcataac cctggccgac atccagcgtg gagccgttga gcctcgcggc cgctatggcg
      181 gcggcgaggg cgcacagcac gcccatcagg acgtaggtct tcaggatcgt ccagcgcgtg
      241 ttgatgccgg ccagctcggc ggcatcggga ttgccgccat aggcgtagac gtagcgaccg
      301 aaccgacgcc gggtcgcgat ccaggtcatc accagcgtca cgccaatgag cagcatgatc
      361 gggtacggga agccgatgga gatgtggagg ccgccggccg ggatcgggat gccgtgctcg
      421 gcagcgtacc gattggccag gcccttcggc cacaggttgg cgttggcgaa tgcggccaat
      481 cccaggacgg ccgcgcagct gatgaagccg agcaacacct ccgcccatac gggccggacc
      541 ggaaagccgt accgccggcg ttgccggcgg ccggtgagga gcaatgcgat gatcgggacg
      601 caggcgacga tgccgaggat ccaggtcgcg gtgcctccga tggatcccag ggctccgccg
      661 ccaatgagct ggaagccgct gtccaggccc gacacggctg cgccgctgga gaggtaccag
      721 atgacgccgc ggatcgagag cagcccgccg agggtgacga tgaatgaagg gaccccgatg
      781 taggcgatga tgaatccctg cagggccccg atggcagccc cgagacccac gccgatgccc
      841 agtgcgatga cccaccggaa cgggaagtcg ggtcccatgc caaggacgtt cggcatccag
      901 tccgtcatga gcagggcgta ggtcatggcg atgacgccga ccaccgaccc gactgacagg
      961 tcgatgtttc gcgacacgat gatcatcacc attccggtgg cgatgatcgc cacgcctgtc
     1021 gcctgcacgg cgagcgtgat catgttcgtc ggctggaaga acttgccccc gctcaggata
     1081 ttgaaggtga gcaggatcaa cgccagggcg acgagcatcc caaagagccg taggtcgatc
     1141 tcggtctgcg cgagcagact gtggagcgag gtccgagcag ggggctggac gtcacctgat
     1201 accggtgtcg cctcggccac gttcgtcacg aaggccctcc tggcctgcga tggagcgacc
     1261 tgcgcccccg ggaatccccg ggggcgcagg tgattgctag gtcagcggtc gatgcccgtt
     1321 acttgcaggc ggccggcgcg gttgccgcgt ccaccccctt gcagaggtca gccttcgaga
     1381 tctgcttggc atcgacggcc gtctgcaggg tgtccgccgt gagcggcgtc ggctgcagga
     1441 tgaaggagct gaccttagtc ccgttcgggg tggtgaactc ctgggcggag agcccagcca
     1501 ccggcgccac ggtcggatcg atcaggccgt cgggcagtgt taggtcggcc gtcgccgtgc
     1561 cgttgcacag cgccagcgca gcggcacctg ccgccttgcc aagttcattg gcgttcttga
     1621 agacgtcgac gtactgcttg ccctgtgcga cgttgttcag gttggcggga tcgccgtcct
     1681 ggccgctcag gggcgggaag ccgtagctct tggccgtgag cgcgcccacg acgccgagcg
     1741 ccgtgctgtc gttttcggcg aggatggcgt cgatcttctt gccgtcggcg acggccttgt
     1801 cgatgatcgc ttccatgttg gcctgggcca acgtggttgt ccaggccgtg gtgaaggtcc
     1861 catcgggccc gttgagaagc acgatgtcgc cggatgcgac cttatccttg aggccggcgt
     1921 cgtcccagcc cttcgggagg aaggtggaag cgttcgcatc gcccgggtcg cccttgatca
     1981 ggacatagtt gcccttcggc accttggtca ggatcgctgt ggcttcggcc ttgccaacca
     2041 ggacgttgtc aaaggtgatg tacaggacgc ctggatcctc gatcaggcgg tcgtaggcga
     2101 tgaccgggat gcccgcatcc ttggccttct ggagggccgg caggatggcc gtgttgctct
     2161 gcgcgagcag gaccagcaca ttggcgccct gcgagatcag cgtgtcgacg tcggtcagct
     2221 gctgctcgct gctgaggttt gcgtctgcac tgatgtagct gccgcctccg tcctccactg
     2281 tcttcttgag gttcggctcg tcgtgtgccg cccagcgcgg ctgctggaag ttgttccagg
     2341 acacgcctac gacgcagggc gccccgctcg gcccggcgct cgcgctgcct ccaacgctcg
     2401 ccgccggcga ctggctggtg caggccgcga gaatcaccgc ggcaagggcg gtcaatgccg
     2461 ccaacctacg aatcgacatg cttggtcctc tcctcgtcag aaagccctga atggtcagag
     2521 tgtgaacctg acgtaattgt cccgtaggct aacaccctcc tcgatcgtgc ggctagcgaa
     2581 ttcatgggtt gccccgcctg tgccagcgag cggttctgga cggggatcgg gggccaggtg
     2641 gagacgtcgc gtggagtggt tagtggggac gcagaaccct gtgctaggct cgtccatcga
     2701 gggcggaact gtcaagccag atctcgcctt ggccggccgc cgccgacaag cctgctggac
     2761 gcctctccac gtgatccgcc ccgttaccgg aggagccgcg cgatgagccc gttggaaggc
     2821 aaggttgccc tggtcacggg ggccagtcga ggcatcggtg cggccaccgc aaggacactg
     2881 gccggtgctg gcgcgcaggt cgcgattgcg tcccgaagcg gtgagaaccc aggcgttgac
     2941 ggcgctctgg cgcaggcgtg cgacgtgagg gaccgggccg cgcttgacgc gatggtggcg
     3001 gccaccgtgg cccggttcgg gcggctggac atcctcgtcg tgaatgccgg cgtgggagcc
     3061 tatgggccat tcctggatct gcccgcggcc gacctggagg agatgatcga cgtcaatgtg
     3121 aagggggcgc tctacgcggt tcgtgctgcc ctccccgagc tgctgaagag cgaggccgcc
     3181 gacatcgtca ccatcgcaag cgaggcaggc cgtcgcgggc tgccgtacga ggtcgcctac
     3241 tgtgcgtcga aattcgcgca ggtggggatg accgccgcgc tcgaccacga gctgcgcgag
     3301 cacggcgtcc ggtgcacgaa tgtgtgcccg ggcggggtgg caaccgactt cgcgatgggc
     3361 catggtcgaa cgccggagat gccgcagctg gccggcatga tggtgccgca ggacgtcgcg
     3421 gaggcggtcc ttttcgtggt cacccgtccg agaagccacc gcatcctcga gattgccttc
     3481 cgaccgatga ccgaatcctc gtgggggtga gcccgctgcc cgagctccga tgggggattc
     3541 tgtccacggc aagcatcgcg cgccagaagg tgatacctgg catccagcgc gccgatcgct
     3601 gccgcgtcgt ggcaatcgcc tcccgcgaca ccgctcgcgc caacgaggtc gcccgcgagg
     3661 cgggcatccc gacggcgcac agctcctacg aggcgctcct cgcggaccca ggcgtggacg
     3721 cggtctacat cccgcttccc aaccatatgc atgccgaatg gacgatcgcc gccgcgcagg
     3781 ccgggaagca cgtcatgtgc gagaagccgc tggcgctgac atcggccgac gcggagcgca
     3841 tggtcgaggc atgcgacgct cacggggttc ggctgatgga ggccctcatg taccgccagc
     3901 acccctcctg ggtggcggtc cgcgagctcg tcgccgccgg acgcatcggg cgtctgacgg
     3961 cggtgcagag ctggttctcg tacttcaacg acgaccccgc tgacatccgc aacgtcggcg
     4021 cattcggggg tggcgcgctc ttcgacatcg gctgctattg cgtcaacctc tccaggatgc
     4081 tgttcgatgg cgagccaacc agggtcgcgt cttccatcgt ccgggatgct gccagcggag
     4141 tggacgtgct gacgagtggg ctcctcgaat tcacgggggg cggcgtcgcg accttcacct
     4201 gcacgacccg agcagaggat gaccagcgcg tgcatatcta cgggacccag ggccgcatct
     4261 cgatcgggat ccctttcaac atccctccag atcgaccgac cgaggtgtac ctgacgatgg
     4321 gcggcgatcc gccggtcgct ccgcataccg agacgatcac gttcgagacc gccgatccgt
     4381 acaccgtcga ggccgagcgc ttcgcgacgg cggtgctcga cggcggcccg acccccgtct
     4441 ccccggccga tgccatcgcc aacctgcgag tcatcgagcg cctcctcgcg gctgatcccc
     4501 gcgcgaccgg tccctcctag caccagacag gggacgaacg ccgatgcaat ctcgtcagct
     4561 ccggatcatc gccgccctgg tcgtttcgct ggcgatcgtc ggtggtggac tcctggccgt
     4621 gctgcggccc tgggagaagc agacgacctg cccgaccgtg gccgtccatc ccgagtggtc
     4681 ggtggcccga cgctgggacg aggcgctgct cgatgccatc cgccgctccc tccctaatcc
     4741 tccggttcat gctcgcaacc tgtaccacct ggcagcggcc atgtgggacg cctgggccgt
     4801 ctatgaccct gtcgccgatg gcgtcttctt cagggagaag cacaccgcat cggacgtcgc
     4861 ggcggcgcgc aacgaggcga tcagctacgc cgcctatcgc gttctttcgg ctcgcttcat
     4921 caaggcagtc ggcgggtccg actcactctc cgagttcgcg gacgtcatgg atgcgctctg
     4981 ctatccgctc gatgtcacga acaccacggg ggatacgccg gccaccattg ggaaccggat
     5041 cgccaagacg gtcctcgcgg ctggcctcct cgacgggtcg aaccaggccg gcgcgtacgc
     5101 cgaccctaac tacgagccgg tcaatgcgcc cctggtggtg ggcgggaccg gggcaacaat
     5161 gatcgacccg aaccgatggc agcccctcca gatcgagcac atgatctccc agaatgggat
     5221 ccccgtgacc aatggggtgc aagtggcgat cgggacgcat tgggggcgcg tcactagctt
     5281 cgcgctcgcc tcggccggcc cgaacggcgt cccgatcgac ccagggccgc cgccactgct
     5341 cggcgacccg gccaccgact cagagctgaa ggcacagctc gtggagctca tccgcgacag
     5401 cagcgagctc gatcccgcca acgatgccac gatcgacatc tcgcccggtg ctcgcggagg
     5461 caacacgctc ggctcgaatg acgggcacgg ccacgctgcc aacccagcga ccgggcagcc
     5521 ttatgcgtcg gacgtcgtca agcgtgcgga cttcggtcgc gtgatggccg agttctgggc
     5581 ggacggcccg aagtccgaga caccgcccgg ccattggaac gtcattgcca atctcgtctc
     5641 ggatgagctg gcgccgaacc tgcggctcgg gggcacgggg ccgcgggtcg gccggctgca
     5701 atgggacgtg aagctctacc tcgccctcaa tggcgccgtg cacgacgcag caattgcggc
     5761 ctggggcctg aagggccact acgactcgag ccggccgatc tcgctcatcc gctatatggg
     5821 cggccttggc cagtcgagcg acccgagcct gtcctcgtac gacccggagg ggctgccgct
     5881 ggtgcccggg ctcatcgagc tgatcacagc ggggacgacg gcccccggag agcgccatgc
     5941 ggctctctcc gggcacgagg gtgccgtggc ggtctatgcg tgggcgggca acccggccga
     6001 tccgaagacc gaggtgggcg gcgccgcatg gatcctggcc acgcgatgga tcccatacca
     6061 gctaccaaca ttcgtgaccc cttccttccc cggctacgcg tcgggccaca gcgccttcag
     6121 ccgcgcggcg gccgaggtgc tgaccgccta caccggcact gagtacttcc ccggcggggt
     6181 cagcgggtac accatcaagg ccgcctccct caagttcgag aagggcccca ccacggatat
     6241 cggcctggag tgggcgacct actacgacgc cgctgaccag gccggccagt cgcggctctg
     6301 gggtggcatc cacatccagg ccgatgattt cacggggcgc atgctcggct cgcaatgcgg
     6361 caaggacgcc tgggcacagg cggagcgcta ttacaccggg accgcgatcc agtgaccgct
     6421 ccggcgtgac gcggggtcgc attggcctcc tggtcgcgct gctggcgttc gcggccatcg
     6481 ccggcgcggc tgtcctgctc cttcgcaccc ccacgccaac tgacgcggca gggccgccgc
     6541 agttcatcga cgagacggct gcctccggcg tgtcccagcg cttcgacggc cccgagacgt
     6601 atcaggtcgg cggcggcgta gcagtcttcg actgcaacgg tgatggcctg ccggacctgt
     6661 atctcggcgg cggcgagggg ccggcgtcgc tcttccggaa ccggtccact gtcggcggag
     6721 cgctccggtt cgatcgcgtc gcgtcgtcga tcactgacct gcccatggtc aacggcgcgt
     6781 acccgctcga cgtcgacggc gacggcgagg ttgacctggc catcctgcgg atcggggaga
     6841 acgtgctgct gcgtggccac ggcgactgca ccttcgaacg ggcgaacgag atgctcgggt
     6901 tctcgggcgt tgacgggtac accacggcgt tcagcgcgac atgggaggga gatgcctcgt
     6961 tgccgaccct ggccatcgga cactatctga aggtcgacag ccacggggat accaccttta
     7021 cctgcgatga gggcgatctg ctgcgcccga atccgggtgg atccggctac gggaccgcgg
     7081 cgcccctcgc tcctggctac tgctcgctgt ccatgctctt cagctcatgg gacggctcga
     7141 gcaggcgcga cctgcgggtt tcgaacgatc gccagtacta caccgatggc tcggagcagc
     7201 tctggcgcat gacgccgggc acgacgccgg gtctctattc agccgacgat ggatgggcgt
     7261 cgctgcagat ctatggcatg gggatcgcca gccaagacct gaccggggac ggcctgcccg
     7321 aggtgtacct caccagccag caggacaaca agctccagac cctcaccgcc ggcagctccc
     7381 agcccgcctt ccgtgacatc gcactgcggc gcggcgtgac ctcgacgcgg ccgttcgccg
     7441 gagataccag cctgccttcg acggcatggc accccgagtt tgcggatgtc aacaacgacg
     7501 ggttcatgga cctcttcgta tcgaagggca acgtcggcgc acaggcggac tacgccatgc
     7561 gcgacccgag cgacctgttc ctcggccagc cggacgggac cttcgtccag gcggctgacc
     7621 gagccggcat ccttcgattc gagcgcggac gcggcgctgc gctggccgac ctcaatctcg
     7681 acggcctgct cgacctggtc gaggtcaact acggagccga tacggtcgtg tggaggaacg
     7741 tcggcgacgg cacggccgac gcgccctcgg cgatgggcca ctggctcatg ctggacatcc
     7801 gacaacccgg cccgaaccgt gacgcgatcg gcgcctgggt ggatgtgcgc atcggcgatg
     7861 tcaccgtcag gcatgagctg acgatcggag gtgggcacct cagcggcgag ctcggctggc
     7921 tccacgtcgg gttgggcccc gcgaccagtg ccgaggtgcg cgtcctatgg cccgatggag
     7981 agcagggtcc ctggctccac gcccaggctg actcgcccgc aatcgtcgag cgcggcgcat
     8041 cggccgtcca gccctggagc ccagcgggag gaggctgatc attggcctgc ttcgcccgga
     8101 ttgcgggatg acccttgtcg cctgacgctc tgctcaccat cgcggacggg ccgatccagg
     8161 tcgagctgct gccggcggtg ggggcaaggc tgcaccggct gcgggccttc ggtcacgatc
     8221 tgctgcgcac gccagacgat ccgacagagc atgtccgcga ccccttccgc tggggcgcct
     8281 acctgatggc gccgtggtgc aaccgcatcg cagcagagcc cgtcgacatc aacggcaaga
     8341 cggtcagcct gccgtccaac ttccccgatg gcaccgcgat ccacggacag gtctacgacg
     8401 cgccatggga tgttcagtcg gacgattccc ttgaaatcat cgcgggcggt gacggctggc
     8461 catggcggta ccgctgctcg ctgcgcgtca cgatcgccga cgcggcgctg acgatcgaac
     8521 agtcactcac caatctcgcc gacagcccga tg
//
LOCUS       DATCQR010000077        12189 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_35084, whole genome shotgun sequence.
ACCESSION   DATCQR010000077 DATCQR010000000
VERSION     DATCQR010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 12189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12189
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35084"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(13..594)
                     /locus_tag="VIN32_02655"
     CDS             complement(13..594)
                     /locus_tag="VIN32_02655"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HEY8921510.1"
                     /translation="MTRVMQPIRLLGIAGSLRSVSFNRALLRAAVEMAPPGVEITTFD
                     ELGALPFYDGDVEAAGEAVGVRRLRAAIDAADGILITTPEYNDGTSAVLKNAIDWASR
                     PPQRTLAGKTAAVMGASVTPSGARGGIESVKRSMRRAGSEVLDEQVAIASVADVFDAD
                     LQLADPLVRAEVAGLVATLAARARAASEERLAA"
     gene            complement(764..1018)
                     /locus_tag="VIN32_02660"
     CDS             complement(764..1018)
                     /locus_tag="VIN32_02660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921511.1"
                     /translation="MTLKVTGPADAELALSAITYSVSVAVIVVPAGDAVVPDEDGAAP
                     DGEAVAPGDDAVEPHAAKTTASPMTAIEGAWLIFTGVCLS"
     gene            1002..1262
                     /locus_tag="VIN32_02665"
     CDS             1002..1262
                     /locus_tag="VIN32_02665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfocyanin-like copper-binding protein"
                     /protein_id="HEY8921512.1"
                     /translation="MTFNVTNAGTMVHEFVILKTDTLAKDLPLVNGVVVEDDFSPVGE
                     VPETAAGKSGTFSATLAAGHYAIICNLAGHVAQGMVIDFTVN"
     gene            complement(1350..2012)
                     /locus_tag="VIN32_02670"
     CDS             complement(1350..2012)
                     /locus_tag="VIN32_02670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921513.1"
                     /translation="MPVFENGWICRECWSANREQDSRCYRCHAVPKRSEAPAATTFST
                     PDGMPKEERKRVSSLTGTPSASTVEPIPTEPVVPIAPRAPRQPVLMPKLRAVNSAVAH
                     GINQGRRLFRVVINAPGAGVSRVTTGVRSGTERVTAAARVAASHKRAWLSVAWIISAF
                     STALLFSVALRAPVAASLLVVASVAIFSGLTSAITTYASERQGRSSDKVPPLLQGLDE
                     VH"
     gene            2160..2645
                     /locus_tag="VIN32_02675"
     CDS             2160..2645
                     /locus_tag="VIN32_02675"
                     /inference="COORDINATES: protein motif:HMM:NF015596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921514.1"
                     /translation="MRLATIMAAGLLLLAACTPPAGAGSAGPSSAGIDVTVSHTSAGD
                     ALAGVGGMTLYIFKADTGGTSTCNAGCVETWAPFLGDGSQVRAGDGVSGSFATTTRDD
                     GSKQITHGGQPLYYYSGDKAAGDSNGEGIAGKWFIAPVDESTASQAATASATPYNPPG
                     Y"
     gene            2677..2913
                     /locus_tag="VIN32_02680"
     CDS             2677..2913
                     /locus_tag="VIN32_02680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921515.1"
                     /translation="MRRRGEQEREEADAHAQAEARREALLAELKAREAADRAPEPPAA
                     EPESSNAEPDEPERPPTDESANSDEGESAQSETA"
     gene            complement(2922..3842)
                     /locus_tag="VIN32_02685"
     CDS             complement(2922..3842)
                     /locus_tag="VIN32_02685"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEY8921516.1"
                     /translation="MRRLVVLLGLALGVGALSACNGGTPSPSDGRLRVVATTTQVGEA
                     ARAVGGGDISLTVLLQPGAEAHEFEITPPAAAAIEKADLILESGAGLETWLQDALATI
                     GGAERLRDMSSGIALRAPDDPAEASDVDPHYWLTAPNAIHLVENVRDALSSARPELAD
                     AFATRAAGYVVRLQAADVEIRRLMAEIPAARRGIVTNHDALGYFMAEYGLRFVGSVFP
                     SLDVSAEPNPAQLAKLADTIRSEGVTAIFSESAVNPRLAKAIAEETGARVVDTPLYTD
                     SLGPPGSAGATLDGMLLHNAQVIHDALVGP"
     gene            complement(3848..4291)
                     /locus_tag="VIN32_02690"
     CDS             complement(3848..4291)
                     /locus_tag="VIN32_02690"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HEY8921517.1"
                     /translation="MDRISLAAADRLSAAGERVTRQRLLVANSLAAAGRQLTAEQLYR
                     SLRRRERGIGRATVFRTLETLVDAGVARRLELDGHIYAYVACLPAHHHHIACTRCGRV
                     EEIDEAYVTPLAERLAQEMGFEIDDARLDFYGRCATCRAEDAAAP"
     gene            4334..5122
                     /locus_tag="VIN32_02695"
     CDS             4334..5122
                     /locus_tag="VIN32_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014280392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEY8921518.1"
                     /translation="MSDKQPGLVLRGVSAGYGAGVVLQDVDLAIPRGTLVGVIGPNGS
                     GKSTLLKAILGLAPQTTGTVLLDGTPVSEQRERLAYVPQREAVDWSFPVSAEQVVMMG
                     RYPRVGWLRIPGAKDAAAVREALERVALADLGSTQIGALSGGQQQRVFLARALVQEAS
                     VLLLDEPMTGVDQTTEQLIVSLLKELRDGGATILQATHDLESAAEISDELCFVNRRVV
                     AYGPPAATFTPSILHETFGGELLIVRAGDQTHVHGGGHHGPVDR"
     gene            5119..6018
                     /locus_tag="VIN32_02700"
     CDS             5119..6018
                     /locus_tag="VIN32_02700"
                     /inference="COORDINATES: protein motif:HMM:NF013143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEY8921519.1"
                     /translation="MSWLTDPLGYDFVLRALAEVVVMGATCGAIGAYVIVRRLAFIGD
                     AISHAVFPGIVLAYLAGLSIFGGALVAGLITALGIALVARGGRIREDTAIGIFFAAAF
                     ALGIVLISTRHTFQGDLTGFLIGNLQGVSAGDIAVSAAVGLAIIVVLAAVHKELVLVS
                     FDRTLASALGYPVFVLDALLLVILTVTIVVSIQAVGIILVLAMLVTPAATARLLVDRF
                     VPMLALGSLLGAVVGVAGYYISFHLGTASGGTIVLLATALFVVVFVASPTHGLLGHRL
                     RRHPQHAGVSPAVDPPPGHEHGA"
     gene            complement(6032..6796)
                     /locus_tag="VIN32_02705"
     CDS             complement(6032..6796)
                     /locus_tag="VIN32_02705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921520.1"
                     /translation="MRTRWRLPAGVLLIGLGLTSIAATVSPLRTAPLFDGVFIEDPFR
                     WVDPPPGAKGDPTSAQADQPVVDGAVPLLAVSTDEVPPQAQIIAQADAFAIAAGTESV
                     KVSIVPSAANDPAIAGNVYRFSVTDALGAPLEIRPEAKVTIVVRASQPNLVAQIARFD
                     GTDWVALPTEFGGLPDLFAANITQLGDFAVVVTGPSPSGSVRASGASSASAAPSGGGG
                     DGPSAEGLPVSLILVLVIAAVGGGLAWGLLGNRERS"
     gene            complement(6796..7386)
                     /locus_tag="VIN32_02710"
     CDS             complement(6796..7386)
                     /locus_tag="VIN32_02710"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEY8921521.1"
                     /translation="MTAVARPAAGRYAATVLWLLAGLAAASLARAALNGHSAASAFAA
                     GAAFGAALVTMGAAAGWRFARPTAASLLAGVLGGAVLIALPRLADPLMPAIIGMRPEP
                     FAGWLLVTTVVVTGEEVLLRGALFGALERSAGPVAAVAVTSLAFALMHVPLYGWQVVP
                     LDLGVGVWLGGLRLATGGVAAPAMAHWLADLSTWWV"
     gene            complement(7383..8729)
                     /locus_tag="VIN32_02715"
     CDS             complement(7383..8729)
                     /locus_tag="VIN32_02715"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8921522.1"
                     /translation="MSRLPRSLILSLCLVVVGCSAGTSKPSAPAGEPIRIGAVFPLGG
                     TAAGLAGYELRGVQIAADLVNADGGIGGRRVQLEVHDLRSRADAPAVMASLRKEGIQA
                     VIGAYGSDLSIAASQAADAAGLVYWEAGAVADRLTGRGLPMVFRVGAAGSNLGSNSAT
                     FAATELASRLGRVTAQLRVAIVAADDAYATSVADAAAATSASFEMPVVARATYSLVIP
                     DWTGLMAELTQARPDVIILASHIPDGIAFRRAMLASGLKVGALIGSTMAECDPDFAGE
                     LGEDAIGIFASDRPTGGFQPDALAGAAHTVYDRFAAAWGSPHIDEGDYGGGSHYGDAE
                     YTISGPAESATAAATEEALSGFTAAWALFHDVLPAAAAAGDLHAAAIARAATRIDLPS
                     GSLPNGAGLHFSSDPSSLGQNERAAAVIWQWQAVSTYAFVWPATFSTGPIGFVPLP"
     gene            complement(8749..9507)
                     /locus_tag="VIN32_02720"
     CDS             complement(8749..9507)
                     /locus_tag="VIN32_02720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921523.1"
                     /translation="MADRAGGGRVLLAGLVMMATAQAVRPVQLPLFDGVVVVDPYRYL
                     SPPPGGEGSPTPARATLPIAAGKSPSFAVYTSETPPQAELLAKGGELALGSGATSVTV
                     TIDPIAPPAGASRDAIAGNVYRFAVMDQSGAALGLAAGQTITLAVRGPPGTAVDATIA
                     RFSDGAWQPVKTAPSGLQDLFLTNAEAFGDYAVVGQVAPSANGLDPKLLLWGLVAAGL
                     IVLVAWRFGGRSSPLVQSNLPAGRRSPRKKRDKT"
     gene            complement(9508..9915)
                     /locus_tag="VIN32_02725"
     CDS             complement(9508..9915)
                     /locus_tag="VIN32_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921524.1"
                     /translation="MRGNRATLAVTVIALVLASCGGQPSASPSVVRPSSPAVITIVEP
                     TAGQTVSGTSVHVVLSLTGAKIVPQTTTKIRPDEGHVHLFVDNVLVSMNYGLEQDLTV
                     HPGTYVLTAEFVAADHAPFSPRVVTPQTIFSVR"
     gene            complement(9912..11876)
                     /locus_tag="VIN32_02730"
     CDS             complement(9912..11876)
                     /locus_tag="VIN32_02730"
                     /inference="COORDINATES: protein motif:HMM:NF017262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopC"
                     /protein_id="HEY8921525.1"
                     /translation="MIRTTRSVAFLALVLVGALGLPHAVLAHALLISSTPAAGATLSE
                     PPTEVVLTFGEAPDPRLTTVRVLDVSGQDQVDGAVVPVAGEPDQLKVRLKPLGDGVFT
                     VAWRTVSAVDGHLAAGSFAFGVGTPPPAPSGETGGGSVVAVSPAATVSRWLFFVGLIA
                     LFGVGFLGFAIEPHLPRSVVGMAGMAWVVFLIGTLGVISVQWQDASVDFRTFIGSSVG
                     TGAVERLAAALVAGIFVGGLSLRSGARRSRFGLVAAVAAATMLVDVLNGHAASGPSWW
                     LQVGVQWVHIGAVGIWVGGLAALLVAVRGLPTEEKARSVRIFSTWAGLGLGAVIGTGF
                     LRALSEVQTIGALFGTSFGIVVILKTVGLGFLALLGATNRFFNVPAAARSLRGLRRVG
                     SVELAIGAGVLAATAVLANLAPPSALGTIPEPAQLPMAAVGSDFGTSVKLSLLVQPGA
                     PGFNQFSATATDYDAGSPLAATSVALRFQLASRSGVGASTLDLPETDSGRFSARGGNL
                     SLDGIWNITATVVGPAGSVEVPLVVATRVGAQPVDVNATPGVPTIYTVHLPDHSTVQV
                     YLDPGGAGSNALHVTFFDPQGNELPVPSATIAVTPDGAAGSVISPRLLEPGHFTAELA
                     VPAGPLSIDIIGQVPGGEPLHAHLEFKVEP"
     gene            complement(11879..>12189)
                     /locus_tag="VIN32_02735"
     CDS             complement(11879..>12189)
                     /locus_tag="VIN32_02735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921526.1"
                     /translation="DYTFHLTGAIHGDPVDITVTSSAETFDTVRGTAEIQFPAKVPSL
                     TEVVTRLARIDARLASADDAQAAADRALLVGAGLGLLGVIVGAVALLVARRGARDAKV
                     "
BASE COUNT         1977 a         4207 c         4246 g         1759 t
ORIGIN      
        1 cctcgcaacc ggttaggcgg cgagcctctc ctcggatgct gcgcgggccc gcgcagcgag
       61 cgttgccacc aggccagcca cctcggcccg aaccagcgga tcggcgagct gcaggtcggc
      121 atcgaagacg tcggcgaccg atgcgatggc cacctgttcg tcgagcacct cggagcctgc
      181 gcggcgcatt gagcgcttga ccgattcgat cccgccgcgg gctccgctcg gcgtcaccga
      241 agcgcccatg acggccgcgg tcttgccggc cagcgtccgc tgtggcggtc gtgaggccca
      301 gtcgatggcg ttcttgagga cggcgctggt gccgtcgtta tattcgggag tggtgatcag
      361 gatgccgtcg gcggcgtcga tcgccgcgcg cagccggcgc accccgaccg cctcaccagc
      421 cgcctcgacg tcgccgtcgt agaagggcag cgcgccgagc tcgtcgaagg tcgtgatctc
      481 gacgcccggt ggcgccatct cgacggcggc gcgcagcagc gctcggttga acgagacgct
      541 gcgcaggctg cccgcgatgc cgagaaggcg aatgggttgc atgactcggg tcatttgatt
      601 gattcctcaa ctatctgacc atatagttgc ataggcaacc atttctgtca aggtcccgac
      661 ggcctgttgg cccccttgaa ggtgccgttt ggcccatccg ccggccatgg ctccacgacg
      721 aagatgcatg cacacgtggc gcaccttgcc cacggcatcc aagttagctg aggcaaaccc
      781 ccgtgaaaat caaccacgcc ccctcgatcg cggtcatcgg gctcgcagtc gtcttggcgg
      841 catgcggctc gactgcgtca tcccccggcg cgaccgcctc gccatcaggc gcggcgccgt
      901 cctcgtccgg cacgaccgcg tcgccggccg gcacgacgat caccgcgacg ctcaccgagt
      961 acgtaattgc gctgagcgcc agctcggcgt ccgcaggccc ggtgaccttc aacgtcacca
     1021 acgccggcac catggtccat gagttcgtca tcctgaagac cgataccctg gccaaggacc
     1081 tgccgctggt caatggcgtg gtggtcgagg acgacttcag ccccgtcggc gaagtgccgg
     1141 agacagccgc tggcaagtcg gggacgttca gcgcgacgct ggccgcaggg cactacgcga
     1201 tcatctgcaa cctcgccggc cacgtggcgc aggggatggt catcgacttc accgtcaact
     1261 gagcggcaga taggtgtcgc gaccgttgga aacacccgcc cggcacgtgc ccggcgggtg
     1321 ttctggatat tggcagcagc cccagaggcc tagtggacct cgtcgagccc ctggaggagc
     1381 ggcgggacct tgtcagagga gcgaccctgg cgctccgagg cgtaggtcgt gatcgccgac
     1441 gtcaacccgc tgaagatcgc cacgctggcc accaccagca gacttgccgc tactggagct
     1501 cggagcgcga ctgagaacag cagcgcagtg ctgaacgcgg agatgatcca tgccacgctc
     1561 agccaggcgc gcttgtgcga agcggcgacc cgagccgcag cggtgacgcg ctcggtcccg
     1621 ctccggacgc cggtcgtcac cctgctgacg cctgcgcccg gggcgttgat gacgactcgg
     1681 aagagccgcc tcccctggtt gatcccatgc gcaacggccg aattcacggc gcggagcttc
     1741 ggcatcaaga cgggctgacg cggcgcgcgg ggggcgatcg gcacaaccgg ctcggtgggg
     1801 atcggctcga cagtcgacgc cgacggggtc ccggtcaggc tggataccct cttgcgctct
     1861 tccttgggca tgccatccgg cgtcgagaag gtcgtggccg ccggcgcctc cgagcgcttg
     1921 ggcacggcgt ggcagcgata gcagcgcgaa tcctgctctc ggttcgcgga ccagcactcg
     1981 cggcagatcc atccgttttc gaagactggc attggacgag cgtagccagc cggcgggccg
     2041 tgtcgatgac gggaaagtac cgtaggccac ttggcggacg acccccgtgg tgtaacgcct
     2101 ggcgtaccct tgagaagagg gcaacttcgt tcccccaatc tgcgacagga gaagaaccga
     2161 tgcgcctcgc cacgatcatg gccgccggcc tgctgctgct cgccgcgtgc actccaccgg
     2221 caggagcggg aagcgccggg ccgagctcgg ccggcatcga tgtcacggtc tcgcacacca
     2281 gcgccggtga cgcacttgcg ggagtcggcg gcatgacgct gtacatcttc aaggccgata
     2341 ccggcggcac cagcacctgc aacgcgggct gcgtcgagac ctgggcgccg ttcctgggcg
     2401 atggctcgca ggtgcgtgcc ggtgatggcg tcagcggctc cttcgcgacg acgacccgtg
     2461 atgacggcag caagcagatc acgcacggcg gtcagccgct ctactactac agcggcgaca
     2521 aggccgcggg cgattcgaac ggcgagggga tcgctggcaa gtggttcatc gcgccggtcg
     2581 acgaatcgac ggcgagccag gccgcgacgg cgtcagcgac cccctacaac cccccgggct
     2641 actgaggcag cgcctcgggc gtagactccg ctcgagatga gacgcagggg cgagcaggaa
     2701 cgcgaagagg ccgatgcgca tgcacaggcc gaggctcgtc gtgaggccct gctcgccgag
     2761 ctgaaggctc gggaggcagc cgatcgggca ccggagccac cagccgctga gcctgagtct
     2821 tcgaatgcgg agccggacga gcccgagcgc ccgccaaccg acgaatcggc gaactcagat
     2881 gagggcgaga gcgcccagtc agagacagcc tgaggcaggc cctatggacc gacgagggcg
     2941 tcgtggatga cctgcgcgtt gtgcaacagc atgccgtcca gcgtcgctcc agccgacccg
     3001 ggagggccaa gcgaatcggt gtagagcggg gtgtcgacga cccgtgcgcc cgtctcctcc
     3061 gcgatcgcct tcgcgaggcg ggggttcacg gctgactcgc tgaagatcgc tgtgaccccc
     3121 tcgctccgga ttgtgtcggc gagctttgcg agttgcgctg ggttcggctc tgcggagaca
     3181 tccaggctcg gaaataccga cccgacgaac cgcaggccgt actcggccat gaaatacccg
     3241 agcgcgtcgt ggttggtgac gatccctcgc ctcgctgccg ggatctcggc catcagccgt
     3301 cggatctcga catcagccgc ctgcagccga acgacgtagc ctgcggcgcg agtcgcgaag
     3361 gcgtccgcca gttccggacg cgcggagctc aaggcgtccc gaacgttctc gacgagatga
     3421 atcgcgttgg gggcggtcag ccagtagtgc ggatcaacgt cgctcgcctc ggccgggtcg
     3481 tcgggcgctc ggagcgcgat cccactgctc atgtcgcgca gccgctcggc gccgccgatc
     3541 gtggccaacg cgtcctgcag ccaggtctcc agcccggcgc cactctccag gatcaggtca
     3601 gccttctcga tggcggctgc cgctggtggg gtgatctcga actcgtgcgc ctcggcgccc
     3661 ggctgcagca gaaccgtcag agagatgtca ccaccgccca ccgctcgcgc cgcctcaccg
     3721 acctgggttg tcgtcgccac gacccggagc cggccgtcac tcggcgaggg ggtcccacca
     3781 ttgcacgcgc tcagcgcccc gacgcccagc gccagcccca ggagcacgac gaggcgtcgc
     3841 actacggtca gggagctgcc gcgtcttcgg cccggcacgt cgcgcagcga ccgtagaagt
     3901 ccagcctggc atcgtcgatc tcgaatccca tctcctgcgc caggcgctct gcgagcggcg
     3961 tcacgtaggc ctcgtcgatc tcctcgaccc gtccacagcg cgtgcacgca atgtggtggt
     4021 ggtgggccgg caggcacgca acgtacgcgt agatgtgacc gtcgagctcc aggcgccggg
     4081 caacgccggc gtccacgagc gtctccagcg tgcgaaagac ggtcgctcga ccgatcccgc
     4141 gctcccgtcg ccgcaggctg cgatacagct gctcggccgt cagctggcgg cctgcggcgg
     4201 caagcgagtt ggcgaccagc agccgttggc gagtgacccg ctccccggcg gcgctgagcc
     4261 gatccgccgc ggccaggctg attcgatcca tgaagagagg ctagcacagg cgagactggg
     4321 tatcatgtcg ggcgtgagcg acaagcaacc cgggctggtc cttcgcggtg tgagcgccgg
     4381 ctatggcgcc ggggtggtgc tgcaggatgt cgacctggcg attccgcggg gaaccctggt
     4441 cggggtgatc gggccgaacg gttcgggcaa gagcacgctc ctcaaggcga tcctgggcct
     4501 cgccccacag accaccggca cggtgctgct ggacggcacc ccggtctccg agcagcgaga
     4561 gcggctcgcg tacgtcccgc agcgtgaggc cgtcgactgg agcttcccgg tgagcgccga
     4621 gcaggtggtg atgatgggtc gctacccgcg tgtgggttgg ctgcgcatcc ccggggcgaa
     4681 ggacgccgcc gcagtgcgcg aggcgctgga acgggtggcg ctggccgacc tcgggtcgac
     4741 gcagatcggt gcgctgtcag gtgggcagca gcaacgcgtg ttcctggcgc gagccctggt
     4801 gcaggaggca tcggtcctgc tcctggacga gccgatgacc ggcgtggacc agacaaccga
     4861 gcagctgatc gtcagcctgc tgaaggagct ccgcgacggc ggggcgacca tcctgcaagc
     4921 cacccacgac ctggagtctg cagcagagat ctcggacgag ctctgcttcg tgaaccgccg
     4981 tgtggtcgcc tatggacctc cggccgcgac gttcacgccg agcatcctgc acgaaacatt
     5041 cggcggcgaa ctgttgatcg tgcgggccgg cgaccaaacc cacgtgcatg gaggcggcca
     5101 tcacgggccg gtcgaccgtt gagctggctg accgatccgc tcggctacga cttcgtcctt
     5161 cgcgcgcttg cggaagtggt ggtgatgggg gcgacctgcg gggcgatcgg cgcgtacgtc
     5221 atcgtgcggc gcctggcctt catcggtgac gcgatcagcc atgccgtctt cccgggcatc
     5281 gtgcttgcct accttgcagg gctgagcatc ttcggcggcg cgctggtcgc gggcctgatc
     5341 accgcccttg gcatcgcgtt ggtggcacga ggcggccgga tccgcgagga cactgccatc
     5401 ggcatcttct tcgccgccgc atttgcactg gggatcgtgc tgatcagcac ccgccatacc
     5461 ttccagggtg accttaccgg cttcctcatc ggcaaccttc aaggtgtctc ggccggggac
     5521 atcgcggtca gtgcggcggt ggggcttgcg atcatcgtcg tgctggcggc ggtacacaag
     5581 gagctggtgc tcgtctcctt cgaccgcacc ctggcctccg cgctcggcta cccggtcttc
     5641 gtgctcgacg cgctgctgct ggtgatcctg accgtgacga tcgtggtgag catccaggcg
     5701 gtcggcatca tcctggtgct ggccatgctc gtcacccctg cggcgaccgc gcgcctgctc
     5761 gtggaccgat tcgtgccgat gctcgcgctc ggatcactcc tcggagcggt cgtgggcgtg
     5821 gccggctact acatctcctt ccacctgggg accgcgtccg gcggcacgat cgtgctgctc
     5881 gcaaccgcac tatttgttgt cgtgttcgtg gcaagcccga cccacgggtt gcttggtcac
     5941 cgcctgcgcc gtcatccgca gcatgctggc gtctcgcccg cggtcgatcc gccgccgggg
     6001 cacgagcatg gcgcgtagat cgactcgggg ctcagctgcg ctcgcggttg ccgaggagcc
     6061 cccatgccag gccaccgccc accgccgcga tcaccagcac gaggatcagc gacacgggga
     6121 gtccctcggc gctcggcccg tcgccgccgc cacccgatgg cgcagctgag gcggagctcg
     6181 cgcccgacgc cctgacggat ccgctggggg atggaccggt gaccaccacc gcgaagtctc
     6241 cgagctgggt gatgttcgcc gcgaacaggt cgggcaggcc tccaaattcg gtggggagcg
     6301 ccacccagtc cgttccatcg aagcgagcga tctgtgcgac caggtttggc tgggaggcgc
     6361 gaaccacgat cgtgaccttc gcctcgggtc gtatctcgag tggtgctccc agcgcatcgg
     6421 tcacgctgaa gcggtagaca ttgccggcaa tggcggggtc gttcgcggcc gatggcacga
     6481 tcgacacctt cacgctctcc gttccggccg cgatggcgaa cgcatcggcc tgggcgatga
     6541 tctgggcctg cgggggcacc tcatcggtgg aaacggcaag gagcggaacg gcgccatcga
     6601 ccaccggctg gtcggcctgg gccgaggtgg gatcaccctt ggctccgggc ggcggatcga
     6661 cccatcgaaa ggggtcctcg atgaacactc cgtcgaagag tggcgccgtg cgcaaaggcg
     6721 aaaccgttgc ggcgatcgag gtcaggccaa gcccgatcag gagcacgccg gccggcagcc
     6781 gccatcgggt gcgcatcaga cccaccaggt gctgaggtcg gcaagccaat gggccatggc
     6841 cggcgccgcg acgccaccgg tcgccagccg caggccgccc agccacactc ctacgccgag
     6901 atcgagcggg acaacctgcc agccgtagag ggggacgtgc atcagggcaa aggcgaggct
     6961 cgtgaccgcg accgccgcca caggacccgc ggagcgctcg agtgccccga acagcgcccc
     7021 ccgcaggagc acctcctcgc cggtgaccac gacagtcgtg acaaggagcc atcccgcaaa
     7081 cggctccggg cgcatcccga tgatcgccgg catcagagga tccgccagcc gtggcagggc
     7141 gatgagcact gcgccaccga gcacgccggc aaggagcgag gcagccgtgg gtcgagcgaa
     7201 gcgccagccg gcggcggcac ccatggtgac cagcgctgcg ccgaacgccg cgcccgcggc
     7261 gaatgcgctc gcagcggaat gtccgttcag ggcggcccgg gccagggacg cggccgccag
     7321 ccccgccagc agccatagga ccgtcgcggc gtagcggccg gccgcgggtc gcgcgaccgc
     7381 ggtcatggca ggggtacgaa gccgatcggc ccggttgaga aggtggccgg ccacacgaaa
     7441 gcgtaggtgc tgaccgcctg ccattgccaa atcaccgcag ccgctcgttc gttctggccg
     7501 aggctcgacg ggtcgctcga gaaatggaga ccggcgccgt tcggaaggga tccgctggga
     7561 aggtcgatcc gcgtggccgc cctcgcgatc gcggcggcgt gcagatctcc agcagcggcc
     7621 gctgccggca gcacgtcgtg gaagagcgcc caggccgccg tgaagccgga gagcgcctct
     7681 tcggttgcgg cggcggtcgc ggactcggcg gggcccgaga tcgtgtattc cgcgtccccg
     7741 tagtgcgagc cgccgccgta gtccccctcg tcgatgtgcg gcgatcccca ggccgcggcg
     7801 aatcggtcgt agacggtgtg cgcggccccg gcgagggcat ctggctggaa gccgcccgtc
     7861 gggcgatccg aggcgaagat cccgatcgcg tcctcgccca gctcaccggc gaagtccggg
     7921 tcgcattcgg ccatggtcga gccgatcagc gcccccacct tgagacccga ggccagcatc
     7981 gcccggcgga atgcgatccc gtcggggatg tgcgaggcga ggatgatcac gtcgggccgc
     8041 gcctgcgtca gctctgccat caggccggtc cagtcgggga tcaccaggct gtaggtggcg
     8101 cgagccacga ccggcatctc aaaggaggcc gatgtcgcgg ctgcggcatc cgcgacggac
     8161 gtcgcatagg cgtcatccgc ggcaacaatc gcgacacgca gctgcgccgt cacgcggcca
     8221 agccgagacg ccagctcggt cgccgcgaag gtcgccgagt tggagccgag gttgctcccc
     8281 gccgcgccga ccctgaagac catcgggagc ccgcgccccg tcaggcgatc agccacggca
     8341 ccagcctccc agtagacgag gcccgccgcg tcggccgcct ggctcgccgc gatcgacagg
     8401 tccgatccat atgcaccgat gacggcctgg atcccctcct ttcgcagcga ggccatgacc
     8461 gccggcgcat cggcacggct tctcaggtcg tgcacctcga gctggacccg ccgtccgccg
     8521 atcccaccgt cggcgttgac caggtcggcc gcgatctgaa ctcctcgcag ctcatagcct
     8581 gccaggccgg ccgcggtccc accgagcggg aagacggccc cgatccggat cggctcgcca
     8641 gcaggagctg acggcttgga tgtgcctgcg ctgcagccga ccaccacgag gcagaggctc
     8701 aggatcaggg agcgtggaag gcggctcatc ggtcctccat gactgtcttc aggtcttgtc
     8761 gcgcttcttg cgaggggaac gacgtccggc cgggaggttc gactgtacga gggggctcga
     8821 ccttccgccg aatcgccagg cgacgagcac gatcagcccg gccgcgacga ggccccagag
     8881 cagtagcttg gggtcgaggc cgttcgcgct gggggccacc tgaccaacga cggcatagtc
     8941 gccgaaggcc tccgcgttgg tgaggaacag gtcctggagt cccgacggcg cggtcttgac
     9001 cggctgccag gctccgtcgc tgaagcgcgc aatggttgcg tccaccgcgg tgccgggcgg
     9061 cccgcgcact gcgagggtga tcgtctgacc cgcggcgagg cccagggcag cgccggactg
     9121 gtccatgacc gcgaagcgat agacgttccc cgcgatcgcg tcccgcgacg cacccgccgg
     9181 tggcgcgatc gggtcgatcg tcactgtcac cgatgtggcc cccgagccga gggcgagctc
     9241 gccacccttg gccagcagct ctgcctgggg cggggtctcg gacgtgtaga ccgcgaagct
     9301 ggggctcttg ccggccgcaa tcggcagcgt tgccctcgcc ggcgttggcg acccttcacc
     9361 accgggtgga ggactgagat accgatatgg gtcgacgacc accaccccgt cgaagagcgg
     9421 aagctggacc ggtcggacgg cctgcgccgt ggccatcatc accaacccgg ccaacagaac
     9481 cctcccgcca ccggctctgt ccgccaccta gcgcaccgag aagatcgtct ggggcgtcac
     9541 cacccgcggg ctgaacgggg cgtggtcggc cgccacgaac tcggcggtca ggacgtacgt
     9601 ccccgggtgg accgtgaggt cctgctccag gccgtagttc atcgacacca acacgttgtc
     9661 gacgaacaga tggacgtgac cctcgtcagg tcggatcttg gtcgtggtct gagggacgat
     9721 cttggcaccg gtgagggaga gcacgacgtg gacgctcgtg ccgctcacgg tctggccggc
     9781 cgtcggttcg acgatggtga tgaccgcggg cgacgacggc cggacgacgc tcgggctggc
     9841 agacggctgc ccgccgcacg aggcgagcac cagtgcgatg accgtgacgg cgagggttgc
     9901 ccggttaccc ctcatggctc gaccttgaac tcgaggtggg cgtgcagggg ttcgccgccg
     9961 ggcacctggc cgatgatgtc gatgctgagg ggcccggccg gcacggccag ctcggccgtg
    10021 aaatgacctg gctcgagcag gcgcggagag atgacggaac cggcggcacc gtccggggtg
    10081 acggcgatcg tcgcactcgg gaccgggagc tcattcccct ggggatcgaa gaaggtcacg
    10141 tggagcgcat tggaacctgc cccgccgggg tcgaggtaga cctggaccgt ggaatggtcg
    10201 ggaaggtgca cggtgtagat ggtcggcacg cccggcgtgg cattcacgtc gacgggctgt
    10261 gcgccgaccc gcgtcgccac tacgagaggc acctcgacgg acccggcagg ccccaccacc
    10321 gtcgcggtga tgttccagat gccgtcaagc gagaggttcc caccgcgggc gctgaagcgg
    10381 ccggaatcgg tctctggcag gtcgagggtc gatgcgccga ctcccgaacg cgaggcgagc
    10441 tggaatcgaa gggcgacgct ggttgcggca agaggggagc cggcgtcgta gtccgtcgcc
    10501 gtcgcggaga actggttgaa gcctggggcg cccggctgga cgaggaggct gagcttgacg
    10561 ctggtgccga agtcgctgcc aacggccgcc atcgggagct gcgctggttc agggatggtg
    10621 ccgagcgcgg agggaggggc caggttggcg aggacggcgg tcgcggccag gacgcctgca
    10681 ccgatcgcca gctcgaccga tccgacccgg cggagcccac gcagcgagcg cgcggctgcg
    10741 gggacgttga agaaccgatt cgtcgcgccg agcagcgcca ggaagccgag cccaacggtc
    10801 ttgaggatca ccacgatccc gaaggaggtc ccgaagagag cgccgatggt ctgcacctcg
    10861 gacagtgcgc gcaggaatcc cgtgccgatg accgcaccaa ggccgaggcc ggcccaggtc
    10921 gagaagatcc gaaccgacct ggccttctcc tcggtcggaa ggccccgcac cgcgaccagc
    10981 agcgccgcga gcccgccgac ccagatcccc actgcgccga tgtgcaccca ttgcacaccc
    11041 acctgcagcc accacgaggg tccgctggcc gcgtggccgt tcaggacgtc cacgagcatc
    11101 gtggccgcgg ccaccgcggc gacgaggccg aaacgcgacc ggcgggcacc agagcgaagc
    11161 gacagtccac caacgaagat cccagcgacc agggcggctg ccaggcgttc gacggcgccg
    11221 gtgccgaccg agctgccgat gaaggtccgg aagtccaccg aagcgtcctg ccactgaacg
    11281 ctgatcaccc cgagggtccc gatcaggaac acgacccagg ccatcccagc cattcccaca
    11341 acggaacgtg gcagatgcgg ttcgatggcg aatcccagga accccacgcc gaagagggcg
    11401 atcagcccca cgaagaagag ccagcggctg accgtcgcgg ccggggagac ggccacgacc
    11461 gatcccccgc cagtctcccc tgaaggcgcg ggcggaggcg tgccgacccc gaacgcgaac
    11521 gaccccgcgg cgaggtggcc gtccaccgcc gagacggtgc gccacgccac ggtgaagacg
    11581 ccatcgccga gtggcttgag gcgcaccttc aactgatccg gctcccccgc caccggcacg
    11641 accgctccgt ccacctgatc ctggcccgag acatcgagca ccctcaccgt ggtcagcctg
    11701 gggtcgggcg cctccccgaa ggtgagcacg acctcggttg gtggctcgct gagcgttgcg
    11761 ccggccgccg gggtcgagga gatcagcagg gcgtgtgcca gcacggcatg ggggagcccc
    11821 agggccccga ccaggacgag cgccaggaat gcgaccgagc gcgtggtccg gatcatggct
    11881 agaccttggc gtcccgtgcg ccgcgcctcg ccaccaagag cgccactgcc ccgacgatca
    11941 cgcccagcag gccgagtccg gcgcccacca agagcgccct gtccgctgcc gcctgggcat
    12001 cgtcggcact cgccagccgc gcgtcgatgc gggcaaggcg ggtcaccacc tcggtcaggc
    12061 tcggcacctt cgcggggaac tggatctcgg cagtcccgcg caccgtgtcg aacgtctcgg
    12121 cggacgaggt cacggtgatg tcgaccggat ccccgtggat cgccccggtc aggtggaatg
    12181 tgtagtcac
//
LOCUS       DATCQR010000078         5142 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_81500, whole genome shotgun sequence.
ACCESSION   DATCQR010000078 DATCQR010000000
VERSION     DATCQR010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5142
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81500"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..975
                     /locus_tag="VIN32_02740"
     CDS             <1..975
                     /locus_tag="VIN32_02740"
                     /inference="COORDINATES: protein motif:HMM:NF021916.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoglucose/phosphomannose
                     isomerase"
                     /protein_id="HEY8921527.1"
                     /translation="LARRVAAAVKLPTSHTEVDGICVLAMGGSAIGAELVEGIAGERL
                     RVPLAVHRDYGLPAWAGERTLVIAASHSGETVETVSGAEEARRRGLPLVVISTGGALG
                     EAAAADGTPYLRYESPGQPRAAIGFGIGLMHELLARAGLLVDPDPLGPAVETLEALLE
                     RNAPSVETDASPAKQLAWSIFGRIPIIYGAGRMAPVAHRWKTQMNENAKAWAAFEPMP
                     EANHNAIEGSLNPRELSDALYVIELRDPTEPPEITARYGVVNELLGERATNRSVVWAE
                     GPSPLARVLSGVAFGDLVSVYLAILYQTDPTPVTLLAMLKQRLARSTD"
     gene            1040..1309
                     /locus_tag="VIN32_02745"
     CDS             1040..1309
                     /locus_tag="VIN32_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HEY8921528.1"
                     /translation="MIGKVEKAMRYAHEPDRVKVERLTASFSGDNGTHTVSLDADTWH
                     CDCHLFESAGGCSHTLAVQKMLDPMLSDAAKDTPLYGRSEAAALA"
     regulatory      1565..1725
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            1754..2710
                     /locus_tag="VIN32_02750"
     CDS             1754..2710
                     /locus_tag="VIN32_02750"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8921529.1"
                     /translation="MTLRLRLLLILSILAMCLAACAPAASTSPSAAGTVAASAPAYPV
                     SLTDDAGRSVNLPGLPRRIVSLAPSNTEIVCALGACDRLVGVTDFDDYPAEVSDVAKV
                     VIGAQVDVEKVVDANPQLILAAGNELTPSSVITQLADLGYPVLVLYPESLDELYADIE
                     LVGRAIDASTEAVDVVDGMRTRAQAVADAVASAEKPRTFYEVGVFQGTIYTAGADSFL
                     SSLISIAGGDPITGDPRSTAIQLEDLVAADPQLILLGDAAYDESITPASVAARDGWKG
                     MTAVIDGHIVVMAEDPVITRPGPRIIDGLEALAHAIHPELFS"
     gene            2722..3825
                     /locus_tag="VIN32_02755"
     CDS             2722..3825
                     /locus_tag="VIN32_02755"
                     /inference="COORDINATES: protein motif:HMM:NF013219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEY8921530.1"
                     /translation="MRAAATTSPRYVTRPSLTRAGLVLGGGGVLLLVTLLAAVAVGSV
                     GVGPGDTAAILGRRLLALPIPETWSPAAETIIFELRLPRVLTAMLVGGGLAMAGTVFQ
                     ALLRNPMADPYIIGTAAGASLGAIIGILAPVLMPALALGAGTAWLGLGMVQALAFAGG
                     IGTVLLVYAVARGNGRVPVVTLLLTGYAVSSVLAAGVALLMFVSGRALGAIFGWLMGS
                     LAGATWQGLAFAVPLMAISFALLLVRWRRLNLLLLGDDQAGHLGVAVEREKLVLTMLA
                     TLATSAAVAISGTIGFVGLVVPHLLRLATGPDHRLLLPASIVYGAALLALADLGARLA
                     GGIPVGIVTALLGAPFFIWLLRRSRDLSRVATA"
     gene            3822..4628
                     /locus_tag="VIN32_02760"
     CDS             3822..4628
                     /locus_tag="VIN32_02760"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8921531.1"
                     /translation="MTVATEALVVARDLVVGFPLPGGERREVLRGVNLTLARGELVAL
                     LGTNGSGKTTLLRTFAGTLVPDRGTVMFEGRSVGDWRRQALAQRVAVLPQQVDLPSGF
                     RVAELVAMGRAPHARRLFASTAEDERAIARALLDADAADLADRPAEELSGGERQRLLV
                     AMALAQEPDLLLLDEPTLHLDLAHQVALLGAIRRLRGQRGLTVLAVLHDLNLAAAFAP
                     RVAILDEGRVVADGPPAEVLSPDLVRRVFGVWVDEARTTNGRRHLALREV"
     gene            complement(4625..>5142)
                     /locus_tag="VIN32_02765"
     CDS             complement(4625..>5142)
                     /locus_tag="VIN32_02765"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEY8921532.1"
                     /translation="LFLVGTLSALAMLIPFFGPPLALIPPIAAAWIYTPDTFIFSAII
                     LIGVQTILVNWLQPRLMQGALGMHPILVLVGLLVGAQVAGVWGALFGIPVIAVLNVFL
                     NLALWSELPNAALPEAERLSDVDEGTMVKVDKAQVSDETHPHIHVHRSVRADGEEQVD
                     ITVDEEEGEER"
BASE COUNT          725 a         1777 c         1810 g          830 t
ORIGIN      
        1 cttgcgcgca gggtcgccgc ggcggtcaag ctgccgacat cgcacaccga ggttgacggt
       61 atctgcgtcc tggcgatggg cggctcggcg atcggcgcag agctggtgga ggggatcgcc
      121 ggcgagcggc tccgggtgcc gctggcagtg catcgcgact acggcctgcc ggcctgggcc
      181 ggcgagcgga cgctggtgat cgcggccagc cattcgggcg agacggttga gacggtctcg
      241 ggggcggagg aggcgcgccg tcgtggcctc ccgctggtcg tcatcagcac cggtggggcg
      301 ctcggagagg ccgcggccgc cgacggcacc ccgtacctcc gctacgagag cccgggccag
      361 ccgcgcgccg cgatcggctt cggcatcggc ctcatgcacg agctgctcgc gcgcgccggc
      421 cttctcgtcg atccagaccc tctgggaccc gccgtggaga ccctcgaggc gctgctcgag
      481 cgaaatgccc cctcggtcga gaccgacgcc agcccggcca agcagctggc ctggtcgatc
      541 ttcggccgga tcccgatcat ctacggtgcc gggcggatgg cccccgtcgc gcaccgctgg
      601 aagacgcaga tgaacgagaa cgccaaggct tgggcggcct tcgagccgat gccggaggcg
      661 aaccacaacg cgatcgaggg gagcttgaac ccgagagagc tgtccgatgc gctgtacgtg
      721 atcgagcttc gcgacccgac cgagccgccg gagatcaccg cccgctacgg cgttgtcaac
      781 gagctgctgg gcgagcgggc gaccaaccga agcgtcgtct gggccgaagg gccgtcgccc
      841 ctggcgcggg tgctgagcgg cgtcgcattc ggcgacctgg tgagcgtgta tctcgcaatc
      901 ctgtaccaga ccgacccgac cccggttaca ctcttggcaa tgttgaagca gcggctcgct
      961 cggtcgaccg actgacgggc gccgatagta acggcgccgg atgccggcgc cgggagggca
     1021 tgcgacggtg cattcgagca tgatcggcaa ggtcgaaaag gcgatgcgct acgcgcacga
     1081 gccggaccgg gtgaaggtag agcggttgac cgccagcttc tccggcgaca acgggacgca
     1141 cactgtctcg ctcgatgccg atacatggca ctgcgattgc cacctgttcg agtccgccgg
     1201 cggctgctcg cataccctgg ccgtccagaa gatgctcgac ccgatgctct ccgacgcggc
     1261 caaggacacg ccactctacg gtcggagcga ggctgccgcc ctggcctagg gtccagcccc
     1321 ggtccgcggg cggacgcccg gtaactgctc cgcctatctc gtcaaatcgc aacctcgctg
     1381 ctcttaggcg agttccggcg cccagccacg cttgtcaagg cattcccgca cgctcaacag
     1441 tgcctcgtgg acggcagctg cgcctcgccc atcgcgccta acgccagtcc acttgcgatt
     1501 tgacgacatc aaggcaatcg cgcctgccca cggcatcgac cttctgatag catcccgggg
     1561 ttaccaactg aagaccgcgt cgggtgccgt ggccgcaagg accacgggtc aatggagaag
     1621 ccggtgagat gccggcacag tcccgctact gtatccgccc cgcgccgccc caccgggcgc
     1681 gcgaggacgg gagtcaggtc gccagcccag gcgcgatgtc cagtcccctc gtgtgaaagg
     1741 gtacggcatc agcatgactc tccgtcttcg gcttctcctc atcctttcga tcctggccat
     1801 gtgcctggct gcctgcgcgc cggcggcaag cacctcaccc agtgccgcgg gaaccgtggc
     1861 cgcctcggca ccggcctatc cggtctccct gaccgatgac gcgggacgct ccgtcaatct
     1921 tcccgggctg ccgcgccgga tcgtcagcct cgcaccctcg aacaccgaga tcgtgtgtgc
     1981 ccttggggcc tgtgaccgtc tggtgggggt gaccgacttc gacgactatc cggccgaggt
     2041 cagcgacgtg gcaaaggtcg tgatcggggc gcaggtcgat gtcgagaagg tcgtggatgc
     2101 caatccgcag ctgatcctgg ccgcgggcaa cgagctgacg cccagcagcg tgatcaccca
     2161 gctcgccgat ctcggctacc ccgtcctggt cctctacccc gagtccctcg acgagctgta
     2221 tgccgatatc gagctggtcg gtcgtgccat cgacgcctcc accgaggcgg tcgacgtggt
     2281 ggacggcatg cgcacccgcg cacaggcagt ggccgatgcc gtcgcgtcgg cggagaagcc
     2341 gcgcaccttc tacgaggtcg gcgtctttca gggcacgatc tacaccgccg gcgcggactc
     2401 attcctcagc tcgctcatct cgatcgccgg cggtgacccg atcaccgggg atccgcgctc
     2461 gaccgcgatc cagctcgagg acctcgtggc cgccgacccg cagctgatcc tgctcggcga
     2521 cgcggcatac gatgagtcca tcacccccgc gagcgtggcc gcccgtgacg gctggaaggg
     2581 gatgaccgcc gtcatcgacg gccacatcgt ggtcatggcc gaggacccgg tcatcacgcg
     2641 tcctggcccg cggatcatcg atggactcga ggcactggcg cacgccatcc atcccgagct
     2701 gttcagctag ccgatccgca gatgcgcgca gctgccacca ccagcccccg ctacgtcacc
     2761 cgcccgtcgc tgacacgggc ggggctggtg cttggtggcg gtggcgtcct gctgctggtc
     2821 accctgctgg cggcggtggc ggtcggcagc gtgggggtcg ggcccggcga cacggcagcg
     2881 atcctcggtc ggcgcctgct ggcattgccg atccccgaga cctggagccc ggcagctgag
     2941 acgatcatct tcgagctgcg cctcccacgc gtcttgacgg cgatgctggt cgggggtggg
     3001 ctggccatgg cagggacggt cttccaggcc ctgctgcgca acccgatggc ggatccctac
     3061 atcatcggca ccgccgccgg cgcgtctctc ggggcgatca tcgggatcct ggctcccgtg
     3121 ctgatgcctg ccctcgcgct tggcgccggg acggcctggc tcgggctcgg gatggtgcag
     3181 gccctggcct tcgccggcgg gatcggcacc gtgctgctgg tgtacgccgt ggcacgcggc
     3241 aacggtcggg tgccggtggt gaccctgttg ctgaccgggt atgccgtctc atcggtgctg
     3301 gcggccgggg tcgcgctctt gatgttcgtg tcgggacgcg cgctgggggc catcttcggc
     3361 tggctgatgg gatcgctggc gggcgccacc tggcagggcc tcgccttcgc ggtgccgctg
     3421 atggccatca gcttcgcctt gctgctcgtt cggtggcgcc ggctcaacct cctgctgctg
     3481 ggcgacgacc aggcggggca cctgggggtc gcggtggagc gcgagaagct ggtgctcacg
     3541 atgctggcca cgctggccac gtcggcggcg gtggcgatct cgggcacgat cggcttcgtt
     3601 ggcctggtgg tcccgcacct cctgcgcctg gcgaccggcc cggatcatcg gctcttgttg
     3661 ccggcgagca tcgtgtatgg cgcggcattg ctggcgctgg cggacctcgg cgcgcggctg
     3721 gcgggcggga tcccggtggg gatcgtcacc gccctcctcg gcgcaccgtt cttcatctgg
     3781 ctgctgcgtc gatcacgcga cctgagcagg gtggcgacgg catgacggtc gcaaccgagg
     3841 cgctggtggt ggctcgcgac ctggtggttg gatttccgct gcccggcggc gagcgacggg
     3901 aggtcctgcg cggcgtgaac ctgacgcttg ctcgcgggga gctggtcgcc ctattgggca
     3961 ccaacggcag cggcaagacc accctcctgc ggacattcgc tggcaccctc gtccccgatc
     4021 gcgggaccgt catgttcgaa ggcagatcgg tcggcgattg gcgtcgacag gcgctcgccc
     4081 agcgcgtcgc ggtcctgccg cagcaggtgg acctgccatc gggctttcgg gtggcggagc
     4141 tggtggcgat ggggcgcgca ccgcacgcgc ggcgactctt cgcctccacc gctgaggacg
     4201 agcgggccat cgcgcgcgcc ctcctggatg ccgatgccgc cgatctggcc gaccgcccgg
     4261 ccgaagagct ctccggcggg gagcgtcagc ggctgctggt ggcgatggcc ctggcgcagg
     4321 agccggacct gcttctgctc gacgagccga cgctgcacct cgacctggcc caccaggtcg
     4381 ccctgctcgg cgcgattcgg cgactgcgcg gccagcgcgg actgaccgta ctggccgtgc
     4441 tgcatgacct gaacctggcc gctgccttcg ccccgcgcgt ggcgatcctc gacgaggggc
     4501 gcgtggtggc ggatgggccg ccagccgagg tgttgagccc agaccttgtg cggcgcgtgt
     4561 tcggggtttg ggtcgacgag gcgcggacga ccaacgggcg tcgccacctg gccctgcgcg
     4621 aggtctagcg ctcctcgccc tcttcctcgt cgacggtgat gtccacctgc tcttcgccgt
     4681 cggcccgcac gctgcggtgg acgtggatgt gcggatgcgt ctcgtcggag acctgcgcct
     4741 tgtcgacctt gaccatcgtc ccctcgtcga cgtcgctcag ccgctcggcc tcgggcagag
     4801 cggcgttcgg cagctcgctc cacagggcga gattcaggaa gacattcagc accgcgatga
     4861 cggggatgcc gaagagcgcg ccccaaaccc cggccacctg ggcgccgacc agcaggccga
     4921 ccagcacgag gatcgggtgc atcccgagcg cgccctgcat gaggcgcggc tgcagccagt
     4981 tgaccaggat cgtctggacg ccgatgagga tgatcgccga aaagatgaag gtgtccggtg
     5041 tgtagatcca ggctgccgcg atcggcggaa tgagggccag cggcgggccg aagaagggga
     5101 tcagcatcgc cagcgcggac agcgtgccga ccaggaagag gt
//
LOCUS       DATCQR010000079         6221 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_128389, whole genome shotgun sequence.
ACCESSION   DATCQR010000079 DATCQR010000000
VERSION     DATCQR010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6221
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128389"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1934)
                     /locus_tag="VIN32_02770"
     CDS             complement(<1..1934)
                     /locus_tag="VIN32_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY8921533.1"
                     /translation="MLTDRPTTATDAIEALLADSALQPLVAAHRVLEPRPPRHAPWPE
                     GLDPRLTHALRSRGVEALYTHQAQSLEAIRAGRNVCVVTPTASGKTLCYNLPVLDAVA
                     RDPTARALYLFPTKALAADQLVELRALADAAELDLKTHTYDGDTPPNVRSVVRAAGQV
                     VITNPDMLHAAILPHHTKWFKLFENLQFVVIDELHTYRGLFGSHVANVIRRLRRITRH
                     YGSDPVFICASATIANPRDLAERILEAPVELIDDNGAPSGRRHILVINPPVANEQLGI
                     RGSALLTGQRVAERLIGGGVQTIAFARSRTSVEVLTTYLRETFAPPPGHPHTIRGYRG
                     GYLPNERKEIERGLRDGRVRGVVATNALELGIDIGGLDAAISIGYPGTIASTWQQMGR
                     AGRRVSTSLSALICSSAPIDQFLAAHPEYLFDTSPEHGLINPDNLHVLLNHLRASSFE
                     LPVPAAERFGVEETPALLDVLEEDGYLRRAEDDRYYWSHENFPASSFSLRSGAPENVV
                     IVDTSGDRHRVLGEVDLFAAPLLVHEKAIYIHEGVQFHVDRLDWDERKAYVTRTDVDY
                     YTDADLGITLKVLEVFEEADQPPAGKRQRGEVMVAWQVTMFKKIKYHTHENVGWGSIS
                     IPEQEMHTTACWLVPPAELV"
     gene            2042..3241
                     /locus_tag="VIN32_02775"
     CDS             2042..3241
                     /locus_tag="VIN32_02775"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
                     /protein_id="HEY8921534.1"
                     /translation="MIRPTPFHPRTSALNETGLWSHWAGHLAANRYQESDKFEYFAVR
                     SAAGIFDTSPLYKYRIAGRDAERFLAGMLARDVRDCPVGHAQYTTWLDDRGFVLEDGV
                     VQHRAPDEYLLTSAEPNFGYFADRIGRLEVQIEDVSSQIGTIAVQGPRSRQLLSKLVP
                     SVTSTPFFGLTDGEIAGVPVTVSRTGYTGDLGYEVWVDAPDALTVWDGLWDAAQGLGV
                     LPFGLAALYMLRIEAGLLLLAVDFDSSRYAFNDAHRSTPIELGWSWMFKGLADDDRAF
                     IGRHALEREIADKTSRWRMSGLVVDWEDYDRVYGEAGLIPPKVHAPVHEDWMVYDGAG
                     QRVGYATSFMYSPVLQRHIALGRVRPDLAKIGQRVQLEFTVDHHYEKVAAHVVRLPFF
                     NPERKTA"
     gene            3238..4833
                     /locus_tag="VIN32_02780"
     CDS             3238..4833
                     /locus_tag="VIN32_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY8921535.1"
                     /translation="MSSEPYDAIVIGGGHNGLVNGAYLARGGLRTLILEQREVVGGAA
                     ITEELRPGFWFTTFSYALSLLRPQIIHELELTKHGFMPLLMSSKFAPTPEGEYLWFTQ
                     HHDQNLREIERISPHDADAYEQYGHDMDRVCQAIKPLLDMVPPDPFSDDPEELLALAG
                     IGSRLRSLDKRTFHNAIRLLTGSAADFLDDYFESDLLKGWMASSGIIGSKVGPRSQGS
                     GLVLLYHSLGESDGEFGSWAFHKGGNGGFTQVLARAAEAFGAEIRLGSTVERVLTREG
                     QATGVALADGTELHAKVVVSALDPRRTFLELVDPRELPDDLVETVRRMKFQGVSSKVN
                     FALSGTPRYPALGDGSDMFRGFLNIAPSMDYLERAYDAAKYGWYSPEPYIDGAIQSTI
                     DPDMAPPGQHVMSCFVQYTPYQLRESDWDAERERLGDTVQSTLERYFPGFGDLVLQRE
                     VVTPLDIERTIGLSEGNIFAGELLAPQMFFLRPAPGWSQYRTPIQGYYQCGSGTHPGG
                     CVMGAPGRLAAGQILKDGGTAHD"
     gene            4911..5339
                     /locus_tag="VIN32_02785"
     CDS             4911..5339
                     /locus_tag="VIN32_02785"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEY8921536.1"
                     /translation="MTSLELPARRRTPPERPLRMRIPASRGGLAWIAVLLIIGTFLAV
                     QVGRQVYTSWSTGQEADAIRAEITAMEAHNDALRQELAYLQSKGFVSAEARRLLNLGL
                     PGEHVLIIPPGAETALPPELRQKPAAKAPLEQWLDLFFGP"
     gene            complement(5356..5856)
                     /locus_tag="VIN32_02790"
     CDS             complement(5356..5856)
                     /locus_tag="VIN32_02790"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HEY8921537.1"
                     /translation="MQVHSGPELAGLLDRLVADDVPNQRGLEAWQALLRAHATLLRQM
                     ESDLVNETGLALADFDVLAQLAHAGGKLRMTELADRALISRSGMTRRVARLVTEGLVA
                     RSGADADARGVVVSLTDAGVERLTETAPVHARGIAKLFVGQLDDQELAVLERALGKVT
                     LDCTFG"
     gene            5931..>6221
                     /locus_tag="VIN32_02795"
     CDS             5931..>6221
                     /locus_tag="VIN32_02795"
                     /inference="COORDINATES: protein motif:HMM:NF014628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HEY8921538.1"
                     /translation="MVTQLRPDLAEALSRQFGTTAEDARVKRTAKALAANGISVLLAS
                     DAAEAKRIVIDLIPEGSEVHEGTSETLAATGITDEIEESGRYRPVRPRIRSMD"
BASE COUNT          988 a         2075 c         2154 g         1004 t
ORIGIN      
        1 ttcaccagct cggccggcgg gacgagccag caggcggtgg tgtgcatctc ctgctcgggg
       61 atgctgatcg agccccaccc gacgttctca tgggtgtggt acttgatctt cttgaacatg
      121 gtcacctgcc aggcgaccat cacctcgccg cgctgtcgct tgccggcggg aggctgatcg
      181 gcctcctcga agacctcgag caccttgagc gtgatgccca ggtcggcatc ggtgtagtag
      241 tcgacgtcgg tgcgcgtgac gtatgccttg cgctcgtccc agtccaggcg atcgacgtgg
      301 aactgcacgc cctcgtggat gtagatcgcc ttctcgtgca cgagcagcgg cgccgcgaag
      361 aggtccacct cgccgaggac gcggtgccgg tcgcccgacg tgtcgacgat gacgacgttc
      421 tccggagccc cgctgcgcag cgagaaggag ctggcgggga agttctcgtg actccagtag
      481 taccgatcgt cctcggcccg ccgcaggtat ccgtcctcct ccaggacgtc gaggagcgcc
      541 ggcgtctctt cgaccccaaa ccgctcggcg gcggggaccg gcagctcgaa gctcgacgcc
      601 cgcaggtggt tgaggagcac gtggaggttg tcagggttga tgaggccgtg ctccggcgaa
      661 gtgtcgaaca ggtactcggg gtgggccgcc aggaactggt cgatcggtgc ggaggagcag
      721 atgagcgccg agaggctggt gctcacccgc cggccggccc ggcccatctg ctgccaggtc
      781 gaggcgatgg tgcccggata gccgatgctg atggcggcgt ccaggccacc gatgtcgatc
      841 cccagctcga gtgcattggt agcgaccacg cctcgcaccc gcccatcgcg aaggccgcgc
      901 tcgatctcct tgcgctcgtt gggcaggtag ccgccgcgat agccgcggat ggtgtgcggg
      961 tgccccggcg gcggagcgaa ggtctcgcgc aggtaggtgg tgagcacctc gacggaggtg
     1021 cgcgaccttg cgaaggcgat cgtctgcacg ccgccgccga tcaggcgctc cgccacgcgt
     1081 tgtccggtga gcagcgccga gccgcgaatg ccgagctgct cgttcgcgac cggcgggttg
     1141 atcacgagga tgtgcctgcg accggaagga gcgccgttgt cgtcaatcag ctcgaccggc
     1201 gcctccagga tccgctccgc caggtcgcgc gggttggcga tcgtggcgct ggcgcagatg
     1261 aataccgggt ccgacccgta gtggcgggtg atgcggcgca ggcgacggat cacgttggcc
     1321 acgtggctgc cgaagaggcc gcggtaggtg tgcagctcgt cgatgaccac gaactgcagg
     1381 ttctcgaaca gcttgaacca cttggtgtgg tgggggagga tcgcggcgtg gagcatgtcc
     1441 gggttggtga tgaccacctg cccggcggcg cgcaccacgg atcgcacgtt cggcggcgtg
     1501 tcgccgtcgt aggtgtgcgt cttcaggtcc agctcggcgg catcggccaa tgcgcgcagc
     1561 tcgaccagct ggtccgcggc gagcgccttg gtggggaaga ggtacaacgc gcgggcggtc
     1621 gggtcgcggg ccacggcgtc caggaccggc aggttgtagc agagcgtctt tcccgatgcg
     1681 gtcggcgtga ccacgcacac gttgcgaccg gcgcggatcg cctcgagcga ctgcgcctgg
     1741 tgcgtgtaca gggcctcgac tccgcgcgaa cgcagggcat gcgtcaggcg cgggtcgagc
     1801 ccctccggcc acggtgcgtg gcgagggggg cgcggctcaa ggacccgatg cgccgccacg
     1861 agcggctgca gggcggagtc ggccaggagg gcttcgatgg catccgtcgc ggtggtgggg
     1921 cggtcggtca gcatgatgcg ctccaaaata gcacgcccgt tctctttcga gcgtgcatct
     1981 caagcatcgc gacgcgcgcg acagctcgcc tgtcgcaccg tgggtgctag ggttccgcct
     2041 gatgattcgc ccgacgccgt tccatccgcg caccagcgcg ctcaacgaga cggggctgtg
     2101 gagccactgg gccgggcacc tggccgccaa ccgctaccag gaatccgaca agttcgagta
     2161 cttcgcggtg cggagcgcgg ccggcatctt cgacacctct ccgctctaca agtaccggat
     2221 cgccggtcgc gatgccgagc gcttcctggc cggcatgctg gcccgcgacg tgcgcgactg
     2281 cccggtcgga cacgcccagt acacgacctg gctcgacgat cgcggcttcg tgctcgagga
     2341 cggggtggtg cagcaccggg cgccggacga gtacctgctc accagcgccg agccgaactt
     2401 cggctacttc gccgatcgca tcggtcgcct cgaggtccag atcgaggatg tgagcagcca
     2461 gatcggcacc atcgcggtgc aaggcccgcg ctcccgtcag ctgctcagca agctggtgcc
     2521 gagcgtgacc tcgaccccct tcttcggctt gacggacgga gagatcgccg gtgtgccggt
     2581 cacggtctca cgcacgggct acaccggtga cctcggctac gaggtgtggg tcgatgcgcc
     2641 tgatgcgctg accgtctggg atgggctgtg ggatgcggcg cagggcctcg gcgtgctgcc
     2701 attcgggctc gccgccctgt acatgcttcg catcgaggct ggcctgctgc tgctggcggt
     2761 cgacttcgat tcgagccgat acgccttcaa cgatgcccat cggtccactc cgatcgagct
     2821 cggctggtcg tggatgttca agggcctggc cgatgacgac cgcgccttca tcgggcgcca
     2881 tgcgctcgag cgcgagattg ccgacaagac ctcacgctgg cggatgagcg ggctggtggt
     2941 cgattgggag gactacgacc gggtctacgg cgaggccggg ctgatcccgc ccaaggtgca
     3001 cgcgccggtc cacgaggatt ggatggtcta cgacggggcc ggccagcggg tcggctatgc
     3061 gaccagcttc atgtactcgc cggtgctgca gcggcacatc gcgctgggtc gggtgcggcc
     3121 ggacctggcg aagatcgggc agcgggtgca gctcgagttc acggtcgacc atcactacga
     3181 gaaggtggcc gcccacgtgg tgcggcttcc gttcttcaac ccggagcgga agacggcatg
     3241 agcagcgagc cgtacgacgc gatcgtcatc ggtggcggcc acaacgggct ggtgaacggc
     3301 gcctacctgg cccgcggcgg gctgcgcacc ctcatcctgg agcagcggga ggtggtcggc
     3361 ggggccgcca tcaccgagga gctgcgcccc ggcttctggt tcaccacctt ctcgtacgcg
     3421 ctctcgctgc tgcggcctca gatcatccac gagctggagc tgacgaagca cggcttcatg
     3481 ccgctcctca tgtcgtcgaa gttcgcaccg acgcccgagg gcgagtacct gtggttcacc
     3541 cagcaccacg atcagaacct gcgcgagatc gagcggatct ctccccacga cgccgatgcc
     3601 tacgagcagt acgggcacga catggaccgc gtctgccagg ccatcaagcc gctgctcgac
     3661 atggtcccgc ccgacccgtt cagcgacgac ccggaggagc tgctcgcact cgcgggcatc
     3721 ggatcgcgac ttcgcagcct cgacaagcgg accttccaca acgccatccg gctcctcacc
     3781 ggcagcgccg ccgacttcct cgacgactac ttcgagtccg atctgctgaa gggctggatg
     3841 gcgtcctcgg ggatcatcgg ctccaaggtc ggaccccgtt cccagggctc cggcctggtg
     3901 ctcctgtacc actcgctggg ggagagtgac ggcgaattcg ggtcctgggc cttccacaag
     3961 ggcggcaacg gcggcttcac ccaggtgctg gctcgcgccg ccgaagcatt cggggcggag
     4021 atccgccttg gatcgaccgt cgagcgggtc ctcaccaggg aagggcaggc gaccggcgtc
     4081 gcactcgccg atgggaccga gctgcacgcg aaggtggtcg tctctgcgct cgacccgcgg
     4141 cgcaccttcc tcgagctggt cgatccccgc gagctgcccg atgacctggt cgagacggtc
     4201 cgccgcatga agttccaggg cgtctcgtcg aaggtgaact tcgccctgag cggcacgcct
     4261 cgctacccgg cgctcggcga cggcagcgac atgtttcgcg gcttcctgaa catcgcgccg
     4321 tcgatggact acctcgagcg ggcctacgac gccgccaagt acggctggta cagtcccgag
     4381 ccgtacatcg atggcgcgat ccagtcgacg atcgacccgg acatggcccc gcccggccag
     4441 cacgtcatgt cctgcttcgt gcaatacacg ccgtaccagc tgcgggagag cgactgggat
     4501 gcggagcgcg aacggcttgg cgacacggtg cagtccaccc tcgagcgcta cttccccggc
     4561 ttcggcgacc tcgtgctgca acgcgaagtg gtgactcccc tggacatcga gcggacgatc
     4621 ggcctgagcg aggggaacat cttcgccggc gagctgttgg cgccccagat gttcttcctc
     4681 cggcccgcgc ccggatggtc gcagtaccgg accccgatcc agggctacta ccagtgcggc
     4741 tcgggaaccc atccgggcgg ctgcgtgatg ggcgctccag gccgcctggc cgccggccag
     4801 atcctcaagg atggcgggac ggcccacgac tgagcgggct cgggtgtgtg gataactctg
     4861 cctggaacgc cttgcacggt ggaaaactgc cgggctagac tggctcccgg atgacaagcc
     4921 tcgaactgcc agcccggcgc cggactcccc ctgagcgacc gctgcggatg cgcatcccgg
     4981 cttcgcgcgg tgggctggcc tggatcgccg tgctgctgat catcggcacc ttccttgccg
     5041 tccaggtggg ccgccaggtc tataccagct ggtcgaccgg ccaggaggca gatgccattc
     5101 gagccgagat caccgcgatg gaggcgcaca acgacgcgct gcgccaggag ctggcctacc
     5161 tccagagcaa gggattcgtg tcagccgagg cgcggcgcct gctgaacctg gggctgcccg
     5221 gcgagcacgt gctgatcatt ccgcccggcg cggagaccgc ccttccaccc gagttgcgcc
     5281 agaagccggc tgccaaggcg ccgctcgagc aatggctcga cctgttcttc ggcccctagc
     5341 ggcgctgagt ggcgcctagc cgaacgtgca gtcgagcgtg acctttccca gggcacgctc
     5401 gaggaccgcc agctcctgat cgtccagctg gcccacgaac agcttcgcaa tcccgcgcgc
     5461 gtggaccgga gcggtctcgg tcagtcgctc gacgccggca tcggtcagtg agacgaccac
     5521 gccacgagca tcggcatcgg cgcccgatcg ggcgacgagc ccctcggtga ccaggcgtgc
     5581 gacgcgacgt gtcatgcccg accgggagat gagggcgcgg tccgcgagct ctgtcatccg
     5641 gagctttccg ccggcatgcg ccagctgagc gaggacatcg aagtcggcga gggccaggcc
     5701 cgtctcgttg acgaggtcgc tttccatctg gcgcaagagg gtggcgtgcg cccggaggag
     5761 tgcctgccat gcctctaggc cgcgctggtt cgggacgtcg tccgcgacga ggcgatcgag
     5821 caggccagcc agctcaggtc cggaatgtac ttgcatatgc aaccaagtta cgagtaggat
     5881 atttgcaacc gcaagtatac cgggacaagg ttcccaaagg aaagcaagac atggtcactc
     5941 aactccgccc cgacctggca gaggcgctca gccgacaatt cggcaccacc gcggaggacg
     6001 cccgcgtgaa gcggacggcg aaggccctgg cggcgaatgg catcagcgtc cttctcgcct
     6061 cggatgccgc cgaggccaag cggattgtca tcgatctcat cccggaagga tccgaggtcc
     6121 atgagggcac gtccgagacg ttggccgcga ccggaatcac cgacgagatc gaggagtccg
     6181 gccgctaccg acccgttcgg ccgcgaatca ggagcatgga c
//
LOCUS       DATCQR010000080         4235 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_19698, whole genome shotgun sequence.
ACCESSION   DATCQR010000080 DATCQR010000000
VERSION     DATCQR010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4235
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19698"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..532)
                     /locus_tag="VIN32_02800"
     CDS             complement(<1..532)
                     /locus_tag="VIN32_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEY8921539.1"
                     /translation="MTDEPLILVERDEERRVALLRFNRPKQLNALNGQVMDELCSALE
                     ALDGDDAIRVLVVTGSERAFAAGADIGEMADASPIDMLRTNRIAQWDRVRRIRKPVIA
                     AVAGWCLGGGCELAMALDLIVAAESARFGQPEINIGVIPGAGGTQRLTRAVGKSVAME
                     MILTGEPIDAREAHRLG"
     gene            complement(529..1488)
                     /locus_tag="VIN32_02805"
     CDS             complement(529..1488)
                     /locus_tag="VIN32_02805"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEY8921540.1"
                     /translation="MAEPSAPLAVDPETLGFERVRYLKDAESGVATVTIDRPQVLNAF
                     DFQTLRELGRAFEDASWDDAMAVVVVTGAGERAFCTGADLDEQAAMGATSGQYWRWMG
                     AFIEMHDRLRNIGKPTIARINGVCVGGGNELQMACDLSVLVDDAYIRHVGPEHGSVPA
                     GGATQWLPILVGDRRAREILMLCEPIWPIQALDWGLVNRVVPRADLDETVAALATNLR
                     AKLPEAMRYTKTQLNWWRDLAWSQTVTHARDWLAIHSTADETREAVAAFHEKRSPRYE
                     ELRRQQVGVGRTCTSCGESGISAYANYCPGCGAQLSDVFESIP"
     gene            complement(1481..1795)
                     /locus_tag="VIN32_02810"
     CDS             complement(1481..1795)
                     /locus_tag="VIN32_02810"
                     /inference="COORDINATES: protein motif:HMM:TIGR02118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EthD family reductase"
                     /protein_id="HEY8921541.1"
                     /translation="MVRLIALYSQPEDPAAFDAHYRDVHDPIVRRYPNLLSMRLTRAD
                     GVGGRPAAFYLMAEMSFASREDLDEALASEPGRESGRDLRNFAQAGVALFVADDEGAT
                     DG"
     gene            complement(1803..2045)
                     /locus_tag="VIN32_02815"
     CDS             complement(1803..2045)
                     /locus_tag="VIN32_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HEY8921542.1"
                     /translation="MTYVIAEPCIGVKDASCVDVCPVDCIHTSDDAPLYYIDPDECID
                     CGACEPECPVTAIFAEDAVPEQWTNFTQINADFFKK"
     gene            complement(2078..3190)
                     /locus_tag="VIN32_02820"
     CDS             complement(2078..3190)
                     /locus_tag="VIN32_02820"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HEY8921543.1"
                     /translation="MPPIALDDATIRRAPKVLLHEHLDGGLRPSTLIELADEIGYAPL
                     PTQDAGELAGWFKPAPGEGGRSLVRYLEAFDHTVAVMQTAAAIERVAAEEVEDLAADG
                     VVYAEVRFAPEQHLRAGLSLDEVVEAAAAGVRRASIGRPIVVGLLLSAMRQAARSVEI
                     AELAVRHRDRGVLGFDVAGPEAGYPPTRYLDAFQLIAHENFHFTIHAGEAFGLPSIWE
                     ALQFCGAERLGHGVRIVDDIVTQPDGRVAIGRLAAYVRDRRIPLEMCPTSNVDTGAVA
                     RLEDHPIDLLKRLRFRVTLNTDNRLMSRISLSDEFASMVATFGWDLADMEWVTLNAIK
                     SAFLPFDGRLRIINEQVKPGYARLRGEASAAEIGLA"
     gene            3255..4130
                     /locus_tag="VIN32_02825"
     CDS             3255..4130
                     /locus_tag="VIN32_02825"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEY8921544.1"
                     /translation="MPERPPLIVAAVTPLAPGGGALDEAAIWPMVRFLEDRGADGVFA
                     CGTTGEGVLLSTEERKQVAVVFRAAVRGRLIVHCGAQTTAETVELSAHAGEIAADAVA
                     VIPPPYFPLDEDALTTHLLAAAEACAPLPFYIYAFAARSGYPVPLEVVARVRDGARNL
                     AGLKVSESPFLKVEPYLDLGLPVYVGSEPLIPAAMARGALGAVSGLAAAFPEVVRDAL
                     DAPDTFAEARLRTLRSAMEMHPFIASVKHVLRRRGVPINPDLRAPLRALTEAEGAALD
                     SVLGELEPAVPLDAA"
BASE COUNT          606 a         1520 c         1448 g          661 t
ORIGIN      
        1 gcccgagccg atgcgcctcg cgggcatcga tcggctcacc ggtcaggatc atctccatcg
       61 ccacgctctt gccgaccgcc cgggtcaggc gctgggtgcc gccggcaccc gggatgacgc
      121 cgatgttgat ctcaggctgg ccgaagcggg cgctctcggc ggcgacgatc aggtccagcg
      181 ccatcgccag ctcgcagccg cctccaagac accagccggc gaccgcggcg atgactggct
      241 tgcggatccg ccgcacgcgg tcccactggg cgattcggtt cgtgcgcagc atgtcgatgg
      301 ggctggcgtc ggccatctcg ccgatgtcgg cgcccgcggc gaaggcgcgc tcgctgccgg
      361 tcacgacgag gacccggatt gcgtcgtccc catccagcgc ctccagggcg gagcacagct
      421 catccatcac ctggccgttc agcgcgttca gctgcttcgg ccggttgaag cggagcagcg
      481 ccacgcgccg ctcctcgtcg cgctcgacca ggatcagcgg ctcgtccgtc acgggatcga
      541 ctcgaacaca tcggagagct gcgcgccgca gcccgggcaa tagttcgcgt acgccgagat
      601 gccgctctcc ccgcaggagg tgcaggttcg gccgacgcct acctgctggc gtcgcagctc
      661 ctcgtagcgc gggctgcgct tctcgtggaa ggcggccacc gcctcgcggg tctcatccgc
      721 cgttgaatgg atggccagcc agtcgcgcgc gtgcgtcacg gtctgcgacc aggccaggtc
      781 acgccaccag ttgagctggg tcttggtgta gcgcatcgct tcaggcagct tggcgcgcag
      841 gttcgtcgcc agcgcggcga ccgtctcgtc caggtcggcg cgagggacga ctcggttgac
      901 gaggccccag tccagcgcct ggatgggcca gatcggctcg cacagcatca ggatctcgcg
      961 cgcccgtcgg tccccgacca ggatcggcag ccactgcgtc gcgccaccgg ccggaacgga
     1021 gccgtgctcg ggaccgacgt gcctgatgta ggcatcgtcg acgagcaccg acaggtcgca
     1081 cgccatctgg agctcgtttc cgccgccgac gcacaccccg ttgatgcgcg cgatcgtcgg
     1141 cttgccgatg ttgcgcaggc ggtcgtgcat ctcgatgaac gcgcccatcc agcgccagta
     1201 ctggccgctg gtggcgccca ttgccgcctg ttcgtcgagg tccgcgccgg tgcagaaggc
     1261 ccgctccccc gccccggtca ccaccaccac ggccatggcg tcgtcccagg aggcgtcctc
     1321 gaaggctcga cccagctcgc gcagcgtctg gaagtcgaag gcgttgagga cctgcggtcg
     1381 gtcgatcgtg accgtcgcca cgccgctttc tgcgtccttg aggtagcgga cccgctcgaa
     1441 gccgagcgtc tccggatcga ccgccagcgg ggcgctcggc tcagccatca gtggcaccct
     1501 cgtcatcggc gacgaacaag gcaacgccgg cctgcgcgaa gttgcgcagg tcgcggccgc
     1561 tctcgcgacc cggctcggag gcaagggcct cgtcgaggtc ctcccgcgac gcgaagctca
     1621 tctcggccat caggtagaag gcggccgggc ggcctcccac tccatcggcc cttgtcagtc
     1681 gcatgctgag caggttgggg tagcggcgca cgattgggtc atgcacgtcg cggtagtgcg
     1741 cgtcgaaggc ggccggatcc tccggctggc tgtagagggc gatcaggcgg accacgaatc
     1801 cgctacttct tgaagaagtc agcgttgatc tgggtgaagt tggtccactg ctccggcacg
     1861 gcgtcctccg cgaagatcgc ggtcaccggg cattccggct cgcaggcgcc gcagtcgatg
     1921 cactcgtcgg gatcgatgta gtacagcggc gcgtcgtcgc tggtatggat gcagtcgacc
     1981 gggcagacgt ccacgcacga ggcgtccttc acgccgatgc acggctccgc gatcacgtac
     2041 gtcatgttgt cgaggataca ccgagcgagc ggcccaccta ggccaggccg atctccgccg
     2101 ccgaggcttc gccccgcagg cgcgcatagc cgggcttcac ctgctcgttg atgatccgca
     2161 gccggccgtc gaagggcagg aatgcgctct tgatggcgtt gagggtgacc cactccatgt
     2221 ccgccaggtc ccagccgaag gtcgcgacca tgctggcgaa ctcgtccgac aggctgatcc
     2281 ggctcatgag ccgattgtcg gtgttcagcg tcacgcggaa gcggaggcgc ttgagcaggt
     2341 cgatcggatg gtcctcgagt cgcgcgacgg caccggtgtc gacgttcgag gtcgggcaca
     2401 tctcgagcgg gatgcgccga tcgcggacat aggcagccag gcgcccgatg gcgacccgcc
     2461 cgtcgggttg cgtgacgatg tcgtcgacga tgcgcacccc atggcccagg cgctcggcgc
     2521 cgcagaactg cagcgcctcc cagatggagg gcagcccgaa ggcctcgccg gcgtggatcg
     2581 tgaagtggaa gttctcgtgg gcgatcagct ggaaggcgtc gaggtagcgt gtgggcgggt
     2641 agcccgcctc cggcccggca acgtcgaagc ccagcacccc gcggtcccga tgacggaccg
     2701 ccagctccgc gatctcgacc gagcgcgctg cctggcgcat cgcgctcagc aacagcccca
     2761 ccacgatcgg ccgcccgatg gatgcgcgcc gcacccctgc agcggccgcc tccaccacct
     2821 cgtcgagcga caggcccgcg cgcaggtgct gctcgggtgc gaagcggacc tcggcgtaca
     2881 ccactccgtc cgccgcgaga tcctcgacct cctcggccgc aacccgttcg atcgccgccg
     2941 cggtctgcat gacggcgacg gtatggtcga atgcctcgag gtagcgcacc agcgagcggc
     3001 cgccctcgcc tggggcgggc ttgaaccagc cggccagctc accggcatcc tgcgtcggca
     3061 gcggggcgta accgatctcg tctgccagct cgatcagggt tgacggccgc agcccgccgt
     3121 ccaggtgctc gtgcagcagc accttgggag cgcggcggat ggtcgcatcg tcgagtgcga
     3181 taggtggcat ggtgtccggc agcctagccc gcctcctatg cggccacaag cggaaagccg
     3241 ctacgcttcc ggccatgccc gaacgacccc cgctcatcgt cgctgccgtg acgccactcg
     3301 cccccggggg cggtgctctg gacgaggccg cgatctggcc gatggtccgc ttcctggagg
     3361 accggggcgc cgacggcgta ttcgcctgcg gcaccaccgg cgagggggtc ctgctcagca
     3421 ccgaggaacg gaagcaggtc gcggtcgtct tccgcgcagc cgttcgtggg cggttgatcg
     3481 tgcactgcgg ggcgcagacg accgccgaaa cggtcgagct gtccgcccac gcgggcgaga
     3541 tcgcggccga tgcggtcgcg gtcatcccgc cgccgtattt cccgctcgat gaggatgcgc
     3601 tcacgactca cctgctggcc gcagccgagg cctgcgcgcc actccccttc tacatctacg
     3661 ccttcgctgc ccggagcggc tacccggtcc cgctcgaggt ggtcgcccgt gtccgcgacg
     3721 gcgccaggaa cctggcgggg ctgaaggtca gcgagtcacc tttcctcaag gtggagccgt
     3781 acctcgacct tggactgccg gtctacgtcg gctctgagcc gctcattccg gcggcgatgg
     3841 ctcgcggggc gctgggcgcg gtgtcaggct tggccgcggc cttccccgag gtcgtgcggg
     3901 acgcgctcga cgcccctgac accttcgccg aggcacgcct ccgcaccctg cgctccgcga
     3961 tggagatgca ccccttcatc gcttcggtga agcacgtcct gcggcggcga ggggtcccga
     4021 tcaacccaga cctgcgcgcg ccgcttcgcg ctctgaccga ggcggaggga gccgcgctcg
     4081 actccgtgct cggcgagctg gagcccgccg tcccgctcga cgcggcctag cccgactcgc
     4141 cggcgtcgcc ggcgacggac accggactgc cggtcgcgat cccggtctcg cgatgatgcg
     4201 gagccgtggc ggcgtcgttc atctcgaacc ggctc
//
LOCUS       DATCQR010000081         1625 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_128506, whole genome shotgun sequence.
ACCESSION   DATCQR010000081 DATCQR010000000
VERSION     DATCQR010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1625
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128506"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..663
                     /locus_tag="VIN32_02830"
     CDS             <1..663
                     /locus_tag="VIN32_02830"
                     /inference="COORDINATES: protein motif:HMM:NF014273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEY8921545.1"
                     /translation="AFLPANRRLRDRWTRIYTAMVEGDELPPIDVYKLDDYYYVIDGH
                     HRVSVARSLGRQEINARVIEVKTRAPVGSHVDGAALLRAAEYSAFLDTTQLHRTRPEA
                     RLECSRLGRYDEILKHILGHRYFLGLEQRREVPLAEAAASWYDNVYTPVIKLIRKYRV
                     LELMPGWTEADLYVEITRRWLQLSEEGAPSGPDPALHSLLDDESRSFWERRRNLKLDR
                     EP"
     gene            660..1232
                     /locus_tag="VIN32_02835"
     CDS             660..1232
                     /locus_tag="VIN32_02835"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013458230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEY8921546.1"
                     /translation="MIGLIFGPPGSGKGTQAAHVEREFKLSHLSTGDILRAEVARGTE
                     IGKEAERIMRAGDLVPDDLIIRIVQGILRDPNVSSDVLLDGFPRTLEQAKALDEMLAQ
                     EGHKVDFVIAFRVPEDVLIERLLHRAEVEGRADDTREAITERMHEYKHLTEKVLDHYH
                     KRGGKVHEIDGVGEIDEIFDRIREALKRGN"
BASE COUNT          294 a          517 c          570 g          244 t
ORIGIN      
        1 gcgttccttc ccgccaaccg gcggctcaga gaccggtgga cccggatcta cacggcgatg
       61 gtggagggcg acgagctgcc gccgatcgac gtgtacaagc tcgacgacta ttactacgtg
      121 atcgacgggc accacagggt ctcggtggct cgaagcctgg gtcgccagga gatcaatgcg
      181 cgggtgatcg aggtgaagac ccgcgcgccg gtgggctctc atgtcgacgg cgcagcgctg
      241 ttgagggcag ccgagtactc tgcgttcctt gacacgacgc agctccaccg cacgcggccc
      301 gaggctcggc tcgagtgcag ccggcttggc cgttacgacg agatcctcaa gcacatcctc
      361 ggccaccgct acttcctcgg gctggagcag cgccgcgagg tcccgctggc cgaggcggcg
      421 gccagctggt acgacaacgt ctacacgccc gtcatcaagc tcatccgcaa gtaccgggtg
      481 ctggagctga tgccgggctg gaccgaggcc gacctttacg tggagatcac caggcgctgg
      541 ctgcagctca gcgaggaggg cgcgccctcg ggcccagacc cggccctgca ttcgctgctg
      601 gacgatgaat cccgaagctt ctgggagcgg cggcggaacc tgaagctgga cagggaaccg
      661 tgatcggtct gatcttcggg ccgcccggca gcggcaaggg cacgcaggcg gctcacgtgg
      721 agcgggagtt caagctgtcg cacctgtcga cgggcgacat cctccgcgcc gaggtggccc
      781 gcggcacgga gatcggcaag gaggccgagc ggatcatgcg ggccggcgac ctggtgccgg
      841 acgacctcat catccgcatc gtccagggca tcctgcgcga ccccaacgtc agctcggatg
      901 tgctgctgga cggtttcccc cggaccctgg agcaggcaaa ggcgctggac gagatgctgg
      961 cccaggaggg ccacaaggtc gacttcgtca ttgcatttag agtcccggag gacgtcctga
     1021 tcgagcggct cctgcatagg gcggaggtgg aggggagggc cgacgacacg cgcgaggcga
     1081 tcacggagcg catgcacgag tacaagcacc tgacggagaa ggtcttggac cactaccaca
     1141 agagaggcgg caaggtgcac gagattgatg gcgtggggga gatcgacgag atcttcgacc
     1201 gcatccggga agcgctgaag cgaggtaatt agagagtcgt cctccccgct tgccggggag
     1261 gtggcgcatc ttccttttgg ggtccccgcg ctcttggtgg gcggggctag cccggagggg
     1321 gcgctcaagc ctgcacatac ggtggctcag gccccagggc cgcaacggag ggccaatctc
     1381 cggaacacca gcctatgctt tgcgctctgg agttcgcgct ggggagcgtg taggacggca
     1441 aagaaggctc gggtagagga tctaggtcac cagaccaagt ccgtcaacgg aaacgggcag
     1501 agcgcgccca gccccgtcgc ggccccggtg gccgcctcgc ccacacagag cgccttcccc
     1561 ccgatcgccg actacgcctt cctgtccgac tgcgaggtca atgcgctgat cgcgccaagc
     1621 ggccg
//
LOCUS       DATCQR010000082         4463 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_4687, whole genome shotgun sequence.
ACCESSION   DATCQR010000082 DATCQR010000000
VERSION     DATCQR010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4463
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4687"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..515)
                     /locus_tag="VIN32_02840"
     CDS             complement(<1..515)
                     /locus_tag="VIN32_02840"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEY8921547.1"
                     /translation="MNASRSPLVAGNWKMHPASAELAVALAVNVRDAVAGVGARSVVC
                     PPTIWLSAVAAAIGGGSTEPGRLGVGAQTMHAEEAGAYTGETSPLMVAEMAQYVILGH
                     SERRQYDNETDAAVAAKVASAVAHGLVPIACIGELAEERRAGLTATVIERQLRAAISL
                     LPRIAGSGLVVA"
     gene            complement(517..1392)
                     /gene="whiA"
                     /locus_tag="VIN32_02845"
     CDS             complement(517..1392)
                     /gene="whiA"
                     /locus_tag="VIN32_02845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HEY8921548.1"
                     /translation="MLVASELRGELARIRPARACCRRAELAGLLQSAGADGTLRTLDH
                     ATARIAVQLAASIGVTAVAPHAFGTGSHVRGRHHLQVEFGGGLRPSWGPVTTKACDRR
                     AFLRGLLLSAGSVSGGPGGPHVEFVLRDRRTAVELQRLLEGSDVRSSRMQRRGREVVY
                     LKGQDEIAGLLRVIGANRALLDFETSRVSREVRNRLNRLLNAEEANLARTVRAADRQL
                     QAISRLDADGELERLADGLREAAGQRRRQPDADLHTLASSLGISRSAMNHRLRRLVEL
                     AADSVGARRARAVRS"
     gene            complement(1405..2685)
                     /locus_tag="VIN32_02850"
     CDS             complement(1405..2685)
                     /locus_tag="VIN32_02850"
                     /inference="COORDINATES: protein motif:HMM:TIGR01826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconeogenesis factor YvcK family protein"
                     /protein_id="HEY8921549.1"
                     /translation="MHLKRWLLLLFFGITVLGLGAAIMLVEFYRGLPDDSLIVTLTGG
                     SLDRPMRAALVALGGIALTSVGVWGLMRSIVSPFVTRGDSVLEVLYTKRYLARGPRIV
                     ALGGGTGLSTLLRGLKGYSANITAIVTLADDGGSSGRLRQQLGIAPPGDIRNCIAALA
                     DAEPLMTQLMQYRFPPGSGLDDHAFGNLFIAAMTAVTGDFDEAVRESNRVLAVRGQVL
                     PATSVPLNLAAILESGRRLKGQASISMADEPIATVSIEPADVRANPEAIERILEADLV
                     VVGPGSLYSSVLPNLLISDIRDAIGAAAGIRAYVCNVATQPGETGFYSAAQHLEALID
                     HVGDGLFDYVLLNDNHNARRPEGWLGQPVQVDVRRLESFDVTIVEEDLVDVSNAHRHD
                     PAKLAAALMRIQQQDRAERPRQRRSVSRPTASAG"
     gene            complement(2721..3668)
                     /gene="rapZ"
                     /locus_tag="VIN32_02855"
     CDS             complement(2721..3668)
                     /gene="rapZ"
                     /locus_tag="VIN32_02855"
                     /inference="COORDINATES: protein motif:HMM:NF015620.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEY8921550.1"
                     /translation="MARAAGPVAPATAPRTSAEASGRTSRARGDGQVVVISGLSGAGK
                     SQASKLFEDLGYYCIDNLPPDLLDRFLALRDEDPARFRNVALVLDIRAGDPAPAIEAA
                     RAALAERGVPMQVLYLEASDTVLVNRFSETRHRHPLETRSGVQAGILLERERLAHARD
                     LADQVIDTSGLSIGQLKEQLFRHVPRDSAADDLLIDILTFGFKFGIPIDADLVFDVRF
                     LTNPYYVADLKPLSGLQTPVREYVLGQPAAGRFIELVVELLELTVPAYRSEGKTRLTV
                     ALGCTGGYHRSIVLAEELAKRLGSLAGVSVAVFHRELER"
     gene            complement(3709..>4463)
                     /locus_tag="VIN32_02860"
     CDS             complement(3709..>4463)
                     /locus_tag="VIN32_02860"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HEY8921551.1"
                     /translation="DAAEQGGGWIDGDTFIGRDSMVAARLAAGATLQAALAAATGEAE
                     IAFAAVRPPGHHAGRERAAGFCLLNNVAVAAMALRAAGLAQRIAILDWDVHHGDGTQS
                     IFDADPDVCYASTHQMPLYPGSGDAAETGVGPGLGSKHNAPLRRGSGDEAFAEAWLTQ
                     LLPAVEAFGPEAILVSAGYDAHRLDPLAELEVTEAGYRSVAEAVGRLARGVGVGGVAL
                     TLEGGYDLDALRDSAAATVAGLLAGLAGSEAA"
BASE COUNT          684 a         1574 c         1568 g          637 t
ORIGIN      
        1 gcgacgacga ggccgctgcc ggcgattcgg ggcagcagcg agatggcggc ccgcagctgt
       61 cgctcgatga cggtggcggt gagtccggct cgacgttcct cggccagctc gccaatgcac
      121 gcgatcggca ccagcccgtg cgcaaccgct gacgcgacct tggcagccac cgccgcatcg
      181 gtctcgttgt cgtactgcct gcgctcggag tgccccagga tgacgtactg ggccatctcg
      241 gcgaccatca gcggggaggt ctcgcctgtg taggcccccg cctcctcggc atgcatcgtc
      301 tgcgccccga cgccgagccg cccgggctcg gtcgatcccc cgccgatcgc ggcagccacc
      361 gcggagagcc agatcgtcgg cgggcagacc accgagcgcg ccccgactcc ggcgaccgca
      421 tcacgcacgt tgaccgccag ggccaccgcc agctccgccg aggcgggatg catcttccag
      481 ttgccggcga cgagtggcga gcgcgatgcg ttcacgtcag ctcctgactg ctcgtgcccg
      541 acgcgcaccg accgaatccg cggccagctc gaccaggcgt cggagccggt ggttcatcgc
      601 cgagcggctg atcccgaggc tggatgcaag cgtatggaga tcggcatccg gctggcggcg
      661 gcgttgtcca gcggcctcac gcagcccgtc ggccaggcgc tcgagctcgc catcggcgtc
      721 caggcggctg atcgcctgca gctgccggtc ggccgcccgc accgtccgcg ccaggttcgc
      781 ctcctcggcg ttcaggaggc ggttgagccg attgcgaacc tcacggctga cccggctcgt
      841 ctcgaagtca aggagggcgc ggttcgcgcc aatcacgcgg aggaggccgg cgatctcgtc
      901 ctggcccttg agatagacca cctcacgtcc gcgacgctgc atgcgggagc tgcgcacatc
      961 actcccctcc agcagtcgct gcagctcaac tgcggtgcgc cggtcgcgaa gcacgaactc
     1021 gacgtgcgga ccgccgggtc cgccgctgac cgagccggcg ctgagcagga gcccgcgaag
     1081 gaaggcgcgc cggtcacagg ccttcgtcgt cacgggtccc caggagggcc gcagcccgcc
     1141 cccgaactca acctgcaggt ggtgccggcc tcggacgtga gagccggttc cgaacgcatg
     1201 cggtgcgact gccgtcacgc cgatcgatgc cgccagctgg acggcgatgc gcgccgtcgc
     1261 gtggtcgagc gtccgaagcg tgccatccgc accggccgat tgcaggaggc ccgccagctc
     1321 ggctcggcga cagcacgccc gggccggacg gatgcgggca agctccccgc gcagctcgct
     1381 ggctaccagc atgcggtgac agtgtcagcc ggcggaggcg gtgggccggg agaccgatcg
     1441 ccgctgccgc ggccgctccg cgcgatcctg ctgctggatg cgcatcagcg ccgccgccag
     1501 cttcgccgga tcatggcggt gcgcattgct cacgtccacg aggtcctcct cgacgatcgt
     1561 gacgtcgaag ctctccaggc ggcgaacgtc aacctgcacc ggctgcccta accagccctc
     1621 gggacggcgc gcgttgtggt tgtcattcag cagcacgtag tcgaagaggc cgtcgccgac
     1681 gtggtcgatg agcgcctcga gatgctgagc agcagagtag aacccggtct cccccggctg
     1741 cgtcgcgacg ttgcagacat acgcccggat ccccgcggcg gccccgatcg catcgcggat
     1801 gtcgctgatc aggagattcg gcaggacgct ggagtagagg ctgccgggac cgaccaccac
     1861 caggtccgcc tccaggatgc gctcgatcgc ctcggggttg gcccgcacgt cggcaggctc
     1921 gatcgagacg gttgcgatcg gctcgtcggc catgctgatg gatgcctgtc ccttgaggcg
     1981 acggccggac tccaggatcg cagccaggtt gagcggcacg ctggtggccg gcagcacctg
     2041 gccccgaacc gccaggacgc ggttcgactc gcgtaccgcc tcgtcgaagt cgccggtgac
     2101 ggcggtcatg gcggcgatga agaggttccc gaaggcatgg tcgtcgagcc ccgagccagg
     2161 cgggaatcgg tactgcatca gctgggtcat gagtggctct gcgtcggcca gtgccgcgat
     2221 gcagtttcgg atgtcgcccg ggggcgcgat ccccagctgc tggcgcagtc ggccgctgct
     2281 ccccccgtca tcggccagcg tgacgatggc ggtgatgttg gctgaatagc ccttgaggcc
     2341 acgcagcagc gtcgacagcc ccgtcccccc gccgagtgcc acgattcgag ggccacgcgc
     2401 caggtagcgc ttggtataga gcacctcgag caccgaatcg ccacgggtga cgaacggcga
     2461 gacgatgctg cgcatcagtc cccagacgcc caccgaggtg agcgcgatcc cgccgagcgc
     2521 caccagggcg gcccgcatcg ggcggtcgag gctccctccg gtgagggtga cgatgagcga
     2581 gtcatcgggc aggccacggt agaactcgac cagcatgatg gcagcaccga gaccgagcac
     2641 ggtgatcccg aagaagagga gcagcagcca gcgcttgagg tgcatcccag ggtagagcca
     2701 gcgagggatg cgcggccgct tcatcgttcc agctcgcggt ggaagaccgc gaccgagacc
     2761 ccagccaggg acccgagccg cttggccagc tcctcggcca ggacgatcga gcggtggtag
     2821 ccaccggtgc agcccagcgc caccgtcagc cgtgtcttgc cctctgaccg gtaggccggc
     2881 acggtaagct cgagcagctc gaccacgagc tcgatgaacc gccccgcagc cggctggccg
     2941 agcacgtact cacggaccgg cgtctggagc cccgacagcg gcttaagatc ggcgacgtag
     3001 tacgggttcg tcaggaagcg gacgtcgaag acgaggtcgg catcgatcgg gatgccgaac
     3061 ttgaagccaa aggtcaggat gtcgatcagc aggtcgtcgg cggcgctgtc acgcggcaca
     3121 tggcggaaga gttgctcctt cagctggccg atcgagaggc cgctggtgtc gatcacctgg
     3181 tctgccagat cacgggcgtg ggccaggcgc tcccgctcga gcaggatccc ggcctgcact
     3241 ccgctgcggg tctcgagggg atggcggtgg cgcgtctcgc tgaagcggtt caccaggacc
     3301 gtgtcgctgg cctcgaggta gaggacctgc atcgggacgc cgcgttcggc gagtgcggct
     3361 cgcgccgcct cgatggccgg ggcgggatcg ccggcccgga tatcgagcac gagggccacg
     3421 ttgcgaaagc gggccgggtc ctcgtcgcgc agggcgagga agcgatccag caggtcgggc
     3481 ggcaggttgt cgatgcagta gtagccgagg tcctcgaaca gcttgctggc ctggctcttg
     3541 cccgccccgg acaggccgct gatcacgacc acctggccgt cgccgcgtgc cctgctggtg
     3601 cgtccgctcg cctcggcgct ggtccgcggg gcggtcgccg gagcgacggg acctgctgct
     3661 cgtgccaatg cgccctcctc gtcgccggga tcgagggcga gtatagcctc aggcagcctc
     3721 cgaaccggcg aggccggcca ggagtccggc gaccgtggcg gcggcgctgt cacgcagggc
     3781 atcgaggtcg tagccaccct ccagggtcaa cgcaacgccc ccgaccccga cacctcgcgc
     3841 caggcgcccg accgcctcgg ccacgctccg gtagccagcc tccgtcacct cgagctccgc
     3901 cagtggatcg agccggtgcg cgtcgtaccc ggccgatacg aggattgcct cgggtccgaa
     3961 ggcttcgacg gcgggaagca gctgcgtcag ccaggcctcg gcgaaggcct cgtcgccact
     4021 tccccgccgg agcggcgcat tgtgcttgct accaagcccc gggccgacgc cggtttcggc
     4081 ggcatcgccg ctcccagggt aaagcggcat ctggtgggtg gacgcgtagc acacgtccgg
     4141 gtccgcgtcg aagatcgact gcgtcccgtc gccgtggtgc acgtcccagt cgaggatcgc
     4201 gatccgctgg gccaggccgg cggcgcgaag cgccattgcc gccaccgcca cgttgttcag
     4261 caggcagaag ccggcggcgc gctcgcgacc agcgtggtgg ccaggcggac ggacagccgc
     4321 gaacgcgatc tctgcctcac ccgtggcagc ggcaagagct gcctggagcg tggctccggc
     4381 ggccagtcgc gcggcgacca tcgaatccct accgatgaac gtgtcgccgt cgatccagcc
     4441 gccgccctgc tcggccgcgt cgt
//
LOCUS       DATCQR010000083         9541 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_35371, whole genome shotgun sequence.
ACCESSION   DATCQR010000083 DATCQR010000000
VERSION     DATCQR010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 9541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9541
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35371"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            31..903
                     /locus_tag="VIN32_02865"
     CDS             31..903
                     /locus_tag="VIN32_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020465141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEY8921552.1"
                     /translation="MSLNRVFVIGAGLMGHGIAQVSAAAGKRVTLADRTAELAEKGKA
                     RIAGNLARQVEKDKLSQEDADALLSRIATAEGATGAGGHEILIEAVFEDESVKRETWQ
                     ALSSAADPGAIFASNTSSISITSLATATDRPGRFIGLHFFSPVPVMSLIEIIRGLETD
                     DATQAAARAFAEELGKTVIESKDYPGFIVNRMLGPFINEAVFALMDGTGSAEHIDAGA
                     KLGLNHPMGPLELSDFIGNDVMLSVLDVLYRGFGDPKFRAAPLLRQMVAAGHLGRKTG
                     RGFYRYDERGTKTG"
     gene            903..2090
                     /locus_tag="VIN32_02870"
     CDS             903..2090
                     /locus_tag="VIN32_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008880728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY8921553.1"
                     /translation="MASTATPTRPDPFALSEEERSVRDTARGFAQRELAPTVAQRDEE
                     ERYDRRLFTRMGELGLTAIPYPEELGGAGLSYFAWALACEEIAAADMGMAISLSVHVL
                     SQLCIVSHGTDEQKSRLLPPMTAGTHLGAFALTEPGAGSDAAALSLAARRTDDGYALT
                     GTKVWITNGGEADVYVVFATVDRSRGSEGITAFVVTPDTPGFRVGSHERKMGIRDSPA
                     AELVFEEALVPEADRLGAEGEGLKVALGSLASGRISIAAACTGLARAALEFATRYASQ
                     RTQFGRPIAEQEMIQAILADAGVAVEASRLLTWRAARLRDAGGSINAASSMAKMFASD
                     TAMRVATDAVQVYGGAGYSRDNPVERLMRDAKGAQIYEGSNQIQRLIIAQHLVEEMQR
                     SDR"
     gene            2090..3979
                     /locus_tag="VIN32_02875"
     CDS             2090..3979
                     /locus_tag="VIN32_02875"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEY8921554.1"
                     /translation="MQTEVEPLDIPAELRADSLVALVHNSMQRNPDKEALRWKLPRTR
                     RQTGGPPEEEPVAWTSTTYRQTWDWITQVAMGLKHLGIRDGDRVCIMSRTRPAWLAAD
                     IGSLSLGAVTCPIYPSVEPNQAAFVINNVEARLIFVENLQQAAKVDQVRAQCPTLEHV
                     VVIDDRGRLPAGAISFDDIFDLAAADAGEVREWSETWRGYGRDKVATIVHTSGTTANP
                     KGVVLTHGNILHNFEGGVAAVDFNEDDLFLSFLPLSHMTERAAGQIVPLGRGCTIAYA
                     EPAIERLAANMAEVRPTVMVAVPRLYERLYARVLSTVEAGPALRQRIFAWALGLGRAK
                     YANHLAGQPNSVWLRLQLKVADRLVFHKIKARTGGRVRYFVSGGAPLSQEIGEFFYAM
                     GMLILEGYGLTETAPLLTINRPGDFKFGTVGRPVAETQIRIDQSTGEILAKGPQVMKG
                     YLNQPEETARVIDADGWFHTGDIGELDPEGRVIITDRLKNIIVLANGKNVAPAPMEIA
                     ILTSRYIAQAVILGDNQPYTGVLVVPDFEQIGTWAATNGMTEMPPEQLVGEKVVQKLI
                     EGEVKAKLDGFAIYERPRRVALLPRLLSEEEGELTPSLKIKTRVVVTKWEDKVAHLFG
                     EDEKE"
     gene            4019..5200
                     /locus_tag="VIN32_02880"
     CDS             4019..5200
                     /locus_tag="VIN32_02880"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEY8921555.1"
                     /translation="MSDVFILSAARTPIGKFGGGLSTVPATELGATAIREAVKRAGIP
                     GERVDEVFMGQVIQAGAGQAPARQAALGAGLPNGVGATTINKVCGSGLKAVMLGAASI
                     RAGDAEMIVAGGMENMNLGPYLLPGARAGYRLGNAELVDATVHDGLWCAICDVHMGVH
                     AERVAAKHDVTRDAQDAFAVRSHERALAAIAEGRFAEEIVAVEVPGKKGPTIVDTDEN
                     PRSDTSLEALARLKPAFQPEGGTVTAGNAPGITDGAAALVLASEAVVASDGLKPIARI
                     TGYAQADVAPEWLFEAPIDGVRRLLTKLDLRLDEFDLIEINEAFAAQVLADGNALGFD
                     WDRVNVNGGAIALGHPIGASGARVLTTLLYELRRRGGRRGLATLCLGGGGAVALAVEM
                     V"
     gene            5316..6584
                     /locus_tag="VIN32_02885"
     CDS             5316..6584
                     /locus_tag="VIN32_02885"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEY8921556.1"
                     /translation="MASSNPVAEAPNIWAVAQAQFDAAAEQLDLDPGLRRVLRVPQRE
                     LTVNFPVTMDDGHVEVFTGFRIQHNVSRGPAKGGIRYHQDVTRDEVRALSMWMTWKCA
                     VVNIPYGGGKGGVIVDPKKLSLRELEGLTRRFTTEISPLIGPDRDIPAPDVNTNAQVM
                     AWMMDTFSMHRGYTIPGVVTGKPIAIGGSLGRNEATARGAVFTLNQASRTLDIPLVGA
                     RVSIQGYGNAGSVAATLLADEGSVIVAVSDSSGGIYNTNGLDPARVSAWKAEHGTVVG
                     FPGADSVSNTEILELDCEILVPAALENQITAENAPRIQARIVAEAANGPTTPEADQIL
                     HDRGVFVIPDILCNAGGVTVSYFEWVQDMQSFFWTESRINESLHEIMDRAFESVHGMS
                     QRHSVDMRTAAYMVAVARVAEATTLRGLYP"
     gene            6595..7200
                     /locus_tag="VIN32_02890"
     CDS             6595..7200
                     /locus_tag="VIN32_02890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921557.1"
                     /translation="MLDHEERFLFREEILNMANALAEAGALDNVEDLLADVTTSPRFD
                     SDVFTLERSLQVMLGSRAGSTLVGLLTVTREVFDEVGEIGLTPEVHERLVGWRARFGL
                     AIDNALNFLNNPDGIQGHNFGTQLTHAEGGLTLQGRLTLVRYNNEPQFFIADADVLLS
                     MAADVMERLGEHLEPEMVEPELMARLREAVELIERRGKPRG"
     gene            7197..8285
                     /locus_tag="VIN32_02895"
     CDS             7197..8285
                     /locus_tag="VIN32_02895"
                     /inference="COORDINATES: protein motif:HMM:NF024891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY8921558.1"
                     /translation="MTAGQRVFVAATGQNRGKTTASLGLTAAIIRRGSNTGFIKPVGQ
                     RYLVVEGIRADEDAVLMKAVFDLPDALDDMSPVTLPRHFTTDYIMGRIEGDLERDVTE
                     AASRVGTGKDLLVIEGTGHAGVGAVVGLSNARVAALLEAPVVIVSEGGVGRPIDEIVL
                     NHALFERDGVRVVGAVVNKVDVESHPDLPDILRRGLAQHGIDLLGCIPYSDLLANPSL
                     ELIATHLDGELLAGQATPGRTIGHVAIGAMQAKHAAGFIRDRTLLITPGDRDDMIEMA
                     LELNAGAEGQVTGIVLTGGFRPPDAMLADLRAASLFTYLVRSDTYGTAQAVDEILVKT
                     HPTDTDKIETIVRLVDGSLDIGDFLAHL"
     gene            8320..8898
                     /locus_tag="VIN32_02900"
     CDS             8320..8898
                     /locus_tag="VIN32_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018187469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HhH-GPD-type base excision DNA repair protein"
                     /protein_id="HEY8921559.1"
                     /translation="MTATQPAVDRLHFTGDDEADRLLASEPLALLIGFALDQQITVQK
                     AFSGPAELRRRIGYLDAARIAAMDPEELQAAFRQRPALHRFPGAMAGRVQALCAFLVE
                     HYGGDASRVWTDARDGRDLQARLLALPSIGDMKARSLIAILGKRFRLSIPGLDEVMPT
                     HPTLGDVDSAEALASYQAGKRAWKAAHRAAKG"
     gene            complement(9056..>9541)
                     /locus_tag="VIN32_02905"
     CDS             complement(9056..>9541)
                     /locus_tag="VIN32_02905"
                     /inference="COORDINATES: protein motif:HMM:NF014611.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEY8921560.1"
                     /translation="HLDGLTTVMLKMLNLVGPASVYLGQRHAQQIVILRRMMRDLFMN
                     IELVVCPTIREADGLALSSANATLSIEERHAATCLFVALNAARAAYDGGERSAEVLRA
                     RMTELIAAESSARPDYVSIADAASLVELQRVTGPALALVAVWIGSTRLTDNMPLAWDV
                     A"
BASE COUNT         1563 a         3281 c         3305 g         1392 t
ORIGIN      
        1 ggattatcgc ggctggtacg ctactcagcc atgagcctca accgggtatt cgtcatcggc
       61 gccggcctca tgggacacgg catcgcccag gtgagcgcgg cagcgggcaa gcgggtgacc
      121 ctcgccgatc gaaccgccga gctggccgag aaggggaagg cacggatcgc gggcaacctg
      181 gcccgccagg tcgagaagga caagctctcc caggaggacg cggacgcgct cctgtcgcgg
      241 atcgccacgg ccgagggtgc aacgggggct ggcggacacg agatcctgat cgaggccgtc
      301 ttcgaggacg aatcggtcaa gcgggagacg tggcaggcgc tcagctcggc cgccgacccc
      361 ggcgcgatct tcgccagcaa cacgagcagc atcagcatca cctcgctggc caccgcgacc
      421 gaccggcccg gccgcttcat cggcctgcac ttcttctcgc ccgtcccagt gatgagcctg
      481 atcgagatca ttcgcggcct tgagaccgat gacgccaccc aggccgccgc tcgcgccttc
      541 gcggaggaac tgggcaagac ggtgatcgag tcgaaggact atcccggatt catcgtcaac
      601 cggatgctcg ggccgttcat caacgaggcg gtcttcgccc tcatggacgg gaccggcagc
      661 gccgagcaca tcgacgccgg ggcgaagctc ggccttaacc acccgatggg cccgctcgag
      721 ctgagcgact tcatcggcaa cgacgtgatg ctcagcgtcc ttgacgtgct gtatcgcggc
      781 ttcggcgacc ccaagttccg agcggccccg ctcctgcgcc agatggtcgc cgcggggcac
      841 ctgggacgaa agacgggccg agggttctat cgctacgacg aacggggcac caagaccggc
      901 tgatggcgag caccgcgacg cccacgcggc cggacccgtt cgccctcagc gaggaggagc
      961 gctctgtccg cgacactgcc cgcggcttcg cgcagcgcga gctggcaccg accgtggccc
     1021 agcgcgacga ggaggaacgg tacgaccgta ggctcttcac ccggatgggc gagctggggc
     1081 tgaccgcgat cccgtacccg gaggagctcg gaggtgcggg gctctcgtac ttcgcctggg
     1141 ctctcgcctg cgaggagatc gccgctgccg acatggggat ggcgatcagc ctctccgtcc
     1201 acgtcctctc ccagctctgc atcgtctccc acggcaccga tgagcagaag tcccgcctcc
     1261 ttccgcccat gacagcgggc acgcacctcg gcgcgttcgc cctgaccgag ccgggggcgg
     1321 gctccgatgc ggcggccctc tccctcgccg cgcgccgcac cgacgacggc tacgcgctga
     1381 ccggcaccaa ggtctggatc accaatggcg gagaggcgga cgtctacgtc gtcttcgcca
     1441 ccgtcgaccg atcgcggggc agcgagggga tcaccgcctt cgtcgtgacc cccgacaccc
     1501 ccggcttccg ggttgggagc cacgaacgca agatggggat ccgggactca ccggccgcgg
     1561 agctggtctt cgaggaggcg ctggtcccgg aggcggaccg gctgggagct gagggtgagg
     1621 gcctgaaggt tgccctcgga tcgctggcgt cggggcggat cagcatcgcg gcggcctgca
     1681 ccggcctggc acgggcagcg ctcgagttcg ccacccgcta cgcctcgcag cgtacccagt
     1741 tcggccggcc gatcgccgag caggagatga tccaggcgat cctggccgat gcgggggtgg
     1801 cagtcgaggc atcgcgcctg ctgacctggc gcgcggcgcg gctgcgtgac gccggaggct
     1861 cgatcaacgc cgccagcagc atggccaaga tgttcgccag cgacacggcc atgcgggttg
     1921 cgaccgatgc ggtgcaggtc tacggcggcg ccggctactc acgcgacaac ccggtcgagc
     1981 gcctcatgcg cgacgcgaag ggagcgcaga tctacgaggg cagcaaccag atccagcggc
     2041 tgatcatcgc gcagcacctg gtcgaggaga tgcagcggtc ggaccggtga tgcagaccga
     2101 ggtcgagccc ctcgacatcc ccgccgagct gcgcgccgac agcctggtgg ccctcgtcca
     2161 caactcgatg cagcgcaacc cggacaagga ggcgctgcgc tggaagctgc ccaggaccag
     2221 gcgccagacc ggtggtcctc cggaggagga gccggtggcc tggacgagca ccacgtatcg
     2281 ccagacctgg gactggatca cgcaggtggc gatgggcctg aagcacctgg gcatccgcga
     2341 cggcgatcgg gtctgcatca tgagccgcac gcgccctgcc tggctggcgg ccgacatcgg
     2401 ctccctctcg ctcggcgccg tgacctgccc gatctacccc tcggtcgagc cgaaccaggc
     2461 cgccttcgtc atcaacaacg tcgaggcgcg cctgatcttc gtcgagaact tgcagcaggc
     2521 ggccaaggtc gaccaggtgc gggcccagtg ccccaccctc gagcacgtgg tggtgatcga
     2581 cgaccgcggg cgcctccccg ccggcgcgat cagcttcgac gacatcttcg acctggccgc
     2641 cgccgatgcg ggagaggtcc gcgagtggtc cgagacctgg cgcggctatg gccgcgacaa
     2701 ggtggcgacc atcgttcaca cctcgggcac caccgccaac ccgaagggcg tggtgctgac
     2761 ccacggcaac atcctgcaca acttcgaggg cggcgtggcc gccgtcgact tcaatgagga
     2821 cgacctgttc ctctccttcc tcccgctctc gcacatgacc gagcgcgcgg cgggccagat
     2881 cgtcccgctc ggccgcggtt gcacgatcgc gtacgccgag cccgccatcg agcgcctggc
     2941 ggccaacatg gcggaggtgc gtcccacggt gatggtcgcg gtcccgcgcc tgtacgagcg
     3001 gctgtatgcg cgggtccttt cgacggtcga ggccggcccg gcgctgcgcc agcggatctt
     3061 cgcgtgggcg ctggggctgg ggcgcgccaa gtacgccaac catctcgccg gccagccgaa
     3121 ctcggtctgg ttgcgcctgc agctgaaggt cgcggatcgg ctcgtcttcc acaagatcaa
     3181 ggcccgcacc ggtgggcggg tgcgctactt cgtgtcgggc ggggctccgc tgtcgcagga
     3241 gatcggcgag ttcttctatg cgatggggat gctgatcctc gaagggtacg ggctcaccga
     3301 gaccgccccg ctgctgacca tcaaccgacc cggcgacttc aagttcggaa cggtcggtcg
     3361 cccggtggcg gagacgcaga tccgcatcga ccagagcacc ggcgagatcc tcgccaaggg
     3421 gccacaggtg atgaagggct acctcaacca gcccgaggag acggcccgcg tcatcgacgc
     3481 cgatggctgg ttccacacgg gcgacatcgg cgagctcgat ccagaagggc gggtcatcat
     3541 caccgatcgg ctcaagaaca tcatcgtgct ggcgaacggg aagaacgtgg cgccggcgcc
     3601 gatggagatc gccatcctga ccagccgcta catcgcgcag gcggtgatcc tcggcgacaa
     3661 ccagccgtac accggggtgc tggtggtgcc cgacttcgag cagatcggga catgggcggc
     3721 cacgaacggg atgaccgaga tgcccccgga gcagctggtc ggagagaagg tggtgcagaa
     3781 gctgatcgag ggcgaggtga aggccaagct cgacggcttc gccatctacg agcgtccgcg
     3841 ccgtgtggcc ctgctgccgc gcctgctctc cgaggaggag ggcgagctga ccccatccct
     3901 caagatcaag acccgggtgg tcgtcaccaa gtgggaggac aaggtcgctc acctgttcgg
     3961 cgaggacgag aaggagtagc ccggcgccgc tcaatgcgcg cggcggtacc attcccgcat
     4021 gagcgacgtc ttcatcctct ccgctgccag aacccccatc ggcaagttcg ggggcggcct
     4081 ctcgaccgtt ccggccacgg agctcggagc cacggccatc cgggaggctg tgaaacgcgc
     4141 aggcataccc ggcgagcgag ttgatgaggt cttcatgggc caggtcatcc aggccggtgc
     4201 tggccaggcg ccggcccgcc aggcggcgct tggcgctgga ctgccgaacg gcgtgggcgc
     4261 caccaccatc aacaaggtct gcggctcagg cctcaaggcg gtgatgctcg gcgccgcctc
     4321 gatccgcgcc ggcgacgccg agatgatcgt ggcgggcggc atggagaaca tgaacctcgg
     4381 tccctacctc cttccggggg ccagggcggg ctatcggctt ggcaacgctg agctcgtcga
     4441 cgccaccgtc catgacggcc tttggtgcgc gatctgcgac gttcacatgg gggtgcacgc
     4501 cgagcgggtg gcggccaagc acgatgtgac ccgcgacgcg caggatgcgt tcgcggtgcg
     4561 ctcccacgag cgagccctgg cggccatcgc cgagggtcgc ttcgccgagg agatcgtcgc
     4621 ggtcgaggtg cccggcaaga aggggccgac catcgtcgac acggatgaga atccgcgatc
     4681 cgatacctcg ctcgaggcgc ttgcgcgcct gaagcctgcc ttccagccgg agggcgggac
     4741 ggtcaccgca ggaaatgcac ctgggatcac cgacggggct gccgcactgg tgctggcctc
     4801 ggaggcggtt gtcgcgagcg acggtctgaa gccgattgcc cgcatcaccg gctatgccca
     4861 ggcggacgtt gccccggaat ggctcttcga ggccccgatc gacggcgtgc gccgtctcct
     4921 caccaagctg gacctgcggc tcgacgagtt cgacctgata gagatcaacg aggcgtttgc
     4981 cgcgcaggtg ctggccgatg gcaacgcgct cgggttcgac tgggatcgcg tcaacgtcaa
     5041 cggcggtgcc atcgcgctgg gccaccccat cggcgcctcg ggcgcccgcg tgctgacgac
     5101 gctcctctat gagctccggc gacgcggggg tcgacgcggg ctggccaccc tgtgccttgg
     5161 cggtggggga gcggtggccc tcgcggtcga gatggtctga gcgccgcctg cgcttgaccc
     5221 aaccctataa tcgggccgcc gcgcccggac cagctcccgc aaccacatcg gcggaggcgt
     5281 cggggcgcga cctgatggag gctcacaggc agtcaatggc aagttccaat ccggtcgccg
     5341 aggcgccgaa catctgggcg gtcgcccagg cccagttcga cgccgcggcc gagcagctcg
     5401 acctcgatcc cggcctgcgt cgcgtgctcc gcgtcccgca gcgcgagctc accgtcaact
     5461 tcccggtgac catggacgat ggccacgtgg aggtcttcac cggcttccga atccagcaca
     5521 acgtcagccg cggccccgca aagggtggca tccgctacca ccaggacgtg acgcgcgacg
     5581 aggtgcgagc tctgtccatg tggatgacct ggaagtgtgc ggtggtgaac atcccctacg
     5641 gcggcggcaa gggtggtgtc atcgtcgatc ccaagaagct ctccctgcgc gagttggagg
     5701 ggctcactcg tcgcttcacg accgagatca gcccgctgat cggtccggac cgtgacatcc
     5761 ccgcaccgga cgtcaacacc aacgcccagg tcatggcctg gatgatggac accttctcga
     5821 tgcatcgcgg ctacaccatc cccggcgtgg tcaccggcaa gccgatcgcg atcggagggt
     5881 cgctgggacg caacgaggcg accgctcgcg gggcggtgtt cacgctgaac caggccagcc
     5941 ggacgctcga catcccgctg gtcggtgccc gcgtgtcgat ccagggatac ggcaacgccg
     6001 gctcggtcgc ggccaccctc ctggcggacg agggctccgt gatcgtcgcc gtcagcgact
     6061 cgagcggcgg catctacaac accaacgggc tcgacccggc acgcgtcagt gcgtggaagg
     6121 cggagcacgg cacggtcgtc ggcttcccag gcgccgactc ggtcagcaat accgagattc
     6181 tcgagctcga ctgcgagatc ctggtcccgg ccgccctcga gaaccagatc accgccgaga
     6241 acgcgcctcg catccaggcc cgcatcgtgg ccgaggccgc caacggcccg accacgcccg
     6301 aggccgacca gatcctccac gaccggggcg tcttcgtgat cccggacatc ctgtgcaacg
     6361 cggggggcgt gacggtcagc tacttcgagt gggtgcagga catgcagtcc ttcttctgga
     6421 cggagtcgcg catcaacgag agcctgcacg agatcatgga tcgtgccttc gagagcgtgc
     6481 acggcatgag ccagcgccac tcggtggaca tgcgcaccgc cgcatacatg gtcgcggtgg
     6541 cgcgggtggc cgaggcgacg acgctgcggg gcctgtaccc ctagactcct gcgcatgctc
     6601 gaccacgaag agcggttcct gtttcgcgag gagatcctga acatggccaa cgccctggcc
     6661 gaggccggcg cgctggacaa cgtcgaggac ctgctcgccg acgtgacgac ctcgccacgg
     6721 ttcgacagcg atgtcttcac gctcgagcgc tccctgcagg tgatgctcgg cagccgtgcc
     6781 ggctccacgc tggttggcct gctcacggtc acccgcgagg tcttcgacga ggtcggcgag
     6841 atcggcctca ctcccgaggt gcacgagcgc ctggtgggct ggcgcgctcg cttcgggctg
     6901 gcgatcgaca acgcgctgaa cttcctgaac aaccccgacg ggatccaggg gcacaacttc
     6961 ggcacgcagc tgacccacgc ggagggcggc ctgacgctgc aggggcggct gaccctggtc
     7021 cgctacaaca acgagccgca gttcttcatc gccgatgccg acgtgctgct gagcatggct
     7081 gccgacgtga tggagcgcct tggcgagcat ctcgagccgg agatggtcga gccggagctg
     7141 atggcccggc tgcgcgaagc ggtcgagctg attgagcggc gcggcaagcc gcgggggtga
     7201 cggcgggcca gcgggtcttc gtggcggcga ccggccagaa ccgtggcaag accaccgcgt
     7261 cgctggggct caccgcggcc atcatccggc gcggatcgaa taccggcttc atcaagccgg
     7321 tcgggcagcg ctacctggtg gtggagggga tccgcgccga cgaggacgcg gtgctcatga
     7381 aggcggtctt cgacctcccc gatgcgctcg acgacatgag cccggtgacc ctgccgcgac
     7441 acttcacgac cgactacatc atgggcagga tcgagggcga cctcgagcgt gacgtcacgg
     7501 aagcggcgag ccgcgtcggc acgggcaagg acctgctggt catcgagggc acgggccacg
     7561 ccggggtcgg tgcggtggtc ggcctctcga atgcgcgcgt ggccgccctg ctcgaggcgc
     7621 cggtggtcat cgtcagtgaa ggcggcgtag ggcgtccgat cgacgagatc gtgctcaacc
     7681 atgcgctctt cgagcgggat ggcgtgcgcg tggtgggagc ggtcgtcaac aaggtcgatg
     7741 tggagtcgca tcccgatctc ccagatatcc tgcggcgcgg cctggcgcag catgggatcg
     7801 atctcctggg ctgcatcccg tacagcgacc tgctggccaa cccctcgctg gagctgatcg
     7861 cgacgcacct cgacggggag ctgctcgccg gccaggcgac cccgggccga acgatcggcc
     7921 acgtggccat cggcgccatg caggcgaagc acgcggcggg attcatccgc gatcggacgc
     7981 tgctgatcac cccgggggat cgggacgaca tgatcgagat ggcgctcgag ctgaacgccg
     8041 gcgctgaggg ccaggtcacc ggcatcgtgc tgaccggcgg ctttcgaccg cccgacgcca
     8101 tgctggcgga cctgcgggcg gccagcctct tcacctacct cgtcagaagc gacacctacg
     8161 gaaccgcgca ggcggtcgac gagatcctgg tcaagacaca ccccaccgac accgacaaga
     8221 tcgagaccat cgtgcgcctc gtcgacgggt cgctcgacat cggggacttc ctggcgcacc
     8281 tctagccgcc gctcacgcgg caggggtagc atgccgcgca tgaccgcaac gcagccagcc
     8341 gtcgatcggc tccacttcac cggcgacgac gaggccgatc gcctgctggc cagcgagccg
     8401 ctggcgctgc tgatcggctt tgcgctcgac cagcagatca ccgtccagaa ggccttctcc
     8461 ggcccagccg agctgcgccg ccgcatcggc tacctggacg ccgcgcggat cgcggcgatg
     8521 gaccccgagg aactgcaggc cgcctttcgg cagcgaccgg cgctgcatcg cttccccggc
     8581 gccatggccg gtcgggtgca ggcgctgtgc gccttcctgg tcgagcacta cggtggcgac
     8641 gcgagtcgcg tctggaccga tgcgcgcgac ggtcgggacc tccaggcgcg gctgctcgcg
     8701 ctgccgtcga tcggcgacat gaaggcccgg tcgctgatcg cgatcctggg caagcgattt
     8761 cgcctcagca tccccgggct cgatgaggtg atgccgaccc acccgacgct gggcgacgtc
     8821 gactcggcgg aagcgctggc cagctaccag gccggcaagc gagcctggaa ggcagcgcac
     8881 cgggcagcca agggctaggg cctgcggccg gacagggaag gaggccggac gtgggcactc
     8941 gccggcctcc tcgttcgccg cggcacagcc ggccgaagcc gacgcgcgcg gtgacagcca
     9001 gcatcatacg gagccgaccc ccgacgtcac tagtactttg tagcccgatt cttcctcagg
     9061 ccacgtccca ggccagcggc atgttgtcgg tcagcctggt gctgccgatc cagaccgcca
     9121 cgagcgccag cgcagggccg gtcacgcgtt ggagctcgac caggctggcg gcgtcggcga
     9181 tgctgacgta gtcgggccgc gccgacgatt cggcggcgat cagctcggtc atccgtgccc
     9241 gcagcacctc ggccgagcgc tcgccgccgt cgtaggcggc tcgagcggcg ttgagggcga
     9301 cgaagaggca ggttgccgca tgacgctcct cgatcgagag ggtggcgttg gcgctcgaca
     9361 gggccaggcc atcggcctcg cggatggtgg ggcagaccac cagctcgatg ttcatgaaca
     9421 ggtcgcgcat catccggcgc aggatcacga tctgctgggc atgtcgctgc cccaggtaca
     9481 cgctggccgg acccaccagg ttcagcatct tgagcatgac cgtggtcagc ccgtccaggt
     9541 g
//
LOCUS       DATCQR010000084         8803 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_19830, whole genome shotgun sequence.
ACCESSION   DATCQR010000084 DATCQR010000000
VERSION     DATCQR010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 8803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8803
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19830"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1269
                     /locus_tag="VIN32_02910"
     CDS             <1..1269
                     /locus_tag="VIN32_02910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921561.1"
                     /translation="LLDEADQRVFARFAVFAGGATLDAVERVVFDADDSADPLDAIAS
                     LVDESLVRQESEPDGEPRFRMLGTIREFAIERLDERGEAEVIGERHAAWVLALVEEGA
                     AAIFGPNQKETLDRYEREHDNIRAALSRAIAYDHAEMAMRIFAASWRFWQMRGYLAEG
                     REHAERVLALPDSREFPDARASALEAAGGIGYWQADILPSRVWYAEALELARASGDEA
                     RIANALYNMTFTWAMEHEDMVQSRAMAHEAVEIYRRLGDEAGTGRALWGLANSYYFFR
                     DFAGGIDVAREALEIFRRVGDRFMTGWSLYIVAIYNMNLDRAKVREALEEALPLFTEI
                     EDKSGYALIFDAFAALDWTEGNVKRAVLLAGYASATEASTGTGLAKMTREFAGFFPTA
                     LVSDPELAAAFAEGQRITVEQATALALPAG"
     gene            complement(1277..2080)
                     /locus_tag="VIN32_02915"
     CDS             complement(1277..2080)
                     /locus_tag="VIN32_02915"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY8921562.1"
                     /translation="MNLRLYLQTLRWNRVRLAAVVVAALGWGMVIPIVYVQFAAAFKD
                     LADSGVFPKELMNFGSGSLFTLPGALTLGLQHPLAIAFVGIFAIGSTVSAIAGERENG
                     TLEVLLSRPISRRTLYVTLAVASATLIAIVLLALLGGQLIGVAIQGVTNELNLGLMPL
                     VFLNGLMLWGAFAAFGLAASVSFDRHAPALGLTLAYLLLNYFLEILGSLWRDVAWSQQ
                     YSLFHHFNPGKILTGSTDWVDFLILAVAISVPVGYALIVFPRRDLAAPS"
     gene            complement(2077..3012)
                     /locus_tag="VIN32_02920"
     CDS             complement(2077..3012)
                     /locus_tag="VIN32_02920"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8921563.1"
                     /translation="MDAEPMGTPQPVIATDSLTKHYGNVRALVDLTLDVRPGEIFGFL
                     GPNGAGKSTMIRTLLGFLHPTRGSATVLGLDVVADSVEIRRRTGYLPGGIALYDALSG
                     ADALEYLAALQDQGSSMREELCERLQMPASVLRRKVRDYSRGMRQKIGVIQAFQHDPE
                     LSILDEPTEGLDPLMQHAFYALMDDQQAKGRTIFFSSHVLSEVERVCDRVAIIRSGHL
                     MALQNVEELLARRKKKVELRWRGAAPDLSGIPGLADVEVKGDRLRATLLGDVSGFVRA
                     IASPSLEDLTIEPARLEEAFLEYYAGDDASEEQRG"
     gene            3157..4365
                     /locus_tag="VIN32_02925"
     CDS             3157..4365
                     /locus_tag="VIN32_02925"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017303334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HEY8921564.1"
                     /translation="MKIAVPRETAPGETRVALTPQAVAALLEDGVAVVVQSGAGDLSF
                     ISDEAFREAGATIVPDAAALYGEADVVVRVGRPSDEETGMLRKGAIIIGTLGTLSNPE
                     LGRRLAETGVTAISMDSIPRITRAQSMDTLSSQATISGYKAVLIAAERLPKFMPLLTT
                     AAGTVRPARGIVMGAGVAGLMAIGTARRLGAVVEATDVRPVVKEQVESLGGIFIEVEM
                     TEEEKANAETAGGYAREMSDDYKARQAALIAERVKEADFVITTALIPGRPAPRLVTDA
                     MIASMKPGSVIVDMAAEAGGNVEGTEAGKEVMKHGVRIIGLTNLPATMPGSATAMYAK
                     NMQTLLKHLIKDGAIALDFTDEITRGATITHGGKVVNEATAKALGIEPSDPAPVAAPG
                     APAAKSGGAA"
     gene            4362..4682
                     /locus_tag="VIN32_02930"
     CDS             4362..4682
                     /locus_tag="VIN32_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006617139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HEY8921565.1"
                     /translation="MNEAVQTLPTEALLQALFVFVLAAFVGYEVVSKVPTMLHTPLMS
                     GANAVHGVIVVGAMLVAASAEGPLRIVLALLGVILGTINVVGGFVVTDRMLEMFKRRP
                     DPKP"
     gene            4679..6094
                     /locus_tag="VIN32_02935"
     CDS             4679..6094
                     /locus_tag="VIN32_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013988291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HEY8921566.1"
                     /translation="MSAMEIVTFFVYLVGSVSFILGLKFLSSPVSARRGNRLAATGMA
                     IVIAWVFITEFLLRDGGLSTVWILLVGIPVGGLIGAIGARRVPMTAMPQMVAIFNGAG
                     GGAAALVAAAEFLRDASDSGPLLPVTFMIATLLGAVIGSVSFTGSIIAFGKLQGIVSG
                     RPVTYPGDKVVTGAVLAGIVVLGVVLTLVTNSVPLFLLFVALALVLGVAAVMPIGGAD
                     MPVVVSLLNAYTGIAVAATGFVLNNYALLVAGTLVGASGTILTQLMCRAMNRSIFNVL
                     FGAFGTAGGGAAAGASGDQDVREISADDAAVLMAYGKKVIIVPGYGMAVAQAQQAVRE
                     LMDLLIAKGVDVEFAIHPVAGRMPGHMNVLLAEANVPYDRLKEMDEINDDFPNASVAL
                     VIGANDVTNPAARTDTSSPIYGMPILNVDQAEHVIVLKRSMASGFAGIDNPLYADPKT
                     TMLFGDAKKSVEALVTGVKAA"
     gene            6274..6942
                     /locus_tag="VIN32_02940"
     CDS             6274..6942
                     /locus_tag="VIN32_02940"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013837472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEY8921567.1"
                     /translation="MIERWVVLDVGETIIDETRVWSIWADMLGIPRLTFLAGFGAVLA
                     RGGEHRDVFELFNVADWRTAWPEHEAIYAGFQPDDLYPDAIPALDALRGRGYRVAVIG
                     NQPASRDEELRRIGVEAEVIAMSAEMGVAKPSQEFFDRVLELLGSPPASSVAYVGDRV
                     DNDVLPAVVAGMRAVWIRRGPWGFIERLPEGVHPALMVASLTELAERIDEAWSGLPEA
                     EPDD"
     gene            7059..8228
                     /locus_tag="VIN32_02945"
     CDS             7059..8228
                     /locus_tag="VIN32_02945"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEY8921568.1"
                     /translation="MEHLPAVLFQLFVLLLATKLGDEVFKRLGQPALVGEILGGVVVG
                     PAVLGWYQITPETSLFAQIGAVLLLFEVGVHTRIGEVARVGGTALSVALLGVALPFVG
                     GYLLGGALDLSAAGRIFLGASLTATSVGISSGALRSFGALATQSGRVILGAAVIDDVL
                     AMLIVAVAVGISDGDVSAGRLLLLLGLAAAFIGIVVVGGTGILRTRRSLLTDPRFAET
                     PFLPGMILMLGLAALAAQIGLAAIIGAFLAGMVVGESSERHALETETAPVAAFFTPFF
                     FGFVGAQIDPAAFGSLPTLGLLAAITLLAIASKFAGSFLGAMRRGRYRAVLIGWGMVP
                     RGEVEIIVAGLALTAGAIKPDLYAVIVGMVVVTAIVVPPLMGPLVRRAEPAIVLE"
     gene            complement(8263..>8803)
                     /locus_tag="VIN32_02950"
     CDS             complement(8263..>8803)
                     /locus_tag="VIN32_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020674886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY8921569.1"
                     /translation="WGVVPDLMTMAKGLTSSYLPLGAVAMSPKIADFFNEKVYFGGLT
                     YSSHPISCAAAIAAVGVLRDEDMVGNAARLDPVMKELLSGLQERHPSVGTVRSIGLFG
                     VFELVRSRDTMEPMAPFNGSSPEMVALEGQLRADGLYTMLHWNQVFTNPPLCITEEQL
                     REGFAVIDRALDATDAAIA"
BASE COUNT         1331 a         2971 c         3076 g         1425 t
ORIGIN      
        1 ctcctggatg aggccgacca gcgtgtcttc gcccgcttcg ccgtcttcgc cgggggagcc
       61 accctggatg ccgtcgagcg ggtcgtcttc gacgccgatg attccgcgga tccgctcgat
      121 gccatcgcct ccctcgtcga cgagagcctg gtccgtcagg agagcgagcc ggacggcgag
      181 ccacgctttc gcatgctggg caccatccgc gagttcgcca tcgagcgcct cgacgagcgc
      241 ggcgaggcgg aggtgattgg ggagcggcac gcggcctggg tcttggcgct tgtcgaggag
      301 ggtgcggccg ccatcttcgg cccgaatcag aaagagaccc tcgaccgcta cgagcgcgag
      361 cacgacaaca tccgtgccgc actctcgcgc gcgatcgcgt acgaccacgc cgagatggcg
      421 atgcgcatct tcgcggcctc gtggcgcttc tggcagatgc gtggctacct ggccgagggg
      481 cgcgaacacg ccgagcgcgt gctggccctg cccgacagca gggagttccc cgacgcgcgc
      541 gcatcggcgc tggaggcagc cggcgggatc ggctattggc aggccgacat cctgccgtcg
      601 cgagtctggt acgccgaggc gctggagctg gcccgcgcct caggtgatga agcccgcatc
      661 gccaacgcgc tctacaacat gaccttcacc tgggcgatgg agcacgagga catggtgcag
      721 tcgcgtgcga tggcgcatga ggcggtcgag atctaccgcc ggctggggga cgaggctggc
      781 accggtcgcg cgttgtgggg cctcgccaac tcgtactact tcttccgcga tttcgcggga
      841 gggatcgacg tcgcccgcga agcgctggag atcttccgtc gcgtcggcga tcggttcatg
      901 actggctggt cgctgtacat cgtcgccatt tacaacatga accttgaccg cgccaaggta
      961 cgggaggcgc ttgaggaggc gctgcccctc ttcaccgaga tcgaggataa gtccggctac
     1021 gcgctgatct tcgacgcctt cgccgcgctc gattggacag aggggaacgt gaagcgcgcg
     1081 gtcctcttgg caggctacgc ttcggctacc gaggcgagca cgggcacggg tctcgccaag
     1141 atgactcgcg agttcgccgg cttcttcccg acggcgctgg tgagcgatcc cgagttggcg
     1201 gcggccttcg ccgaaggcca gcggatcacc gtcgagcagg ccacggcgct cgccctaccc
     1261 gctggttagg cgaacctcag ctcggggcgg cgaggtcccg gcgcgggaaa acgatcaggg
     1321 cgtagccgac cgggacgctg atcgccactg ccaggatcag gaagtccacc cagtccgtgc
     1381 tgccggtcag gatcttgccc ggattgaagt ggtggaagag tgagtattgc tgcgaccagg
     1441 ccacgtcgcg ccagagcgag cccaggatct ccaggaagta gttgagcagc aggtaggcca
     1501 gggtcaggcc cagcgccgga gcgtggcggt cgaagctgac cgacgccgcc agcccgaagg
     1561 cggcgaaggc gccccacagc atcagcccgt tgaggaagac gagcggcatg aggcccaggt
     1621 tgagctcatt cgtgaccccc tggatcgcaa cgccgatcag ctgacctccc aggagcgcaa
     1681 gcaggacgat cgcaatgagg gtcgccgatg ccacggccag cgtcacgtac agagtccgtc
     1741 gcgagatcgg tcgcgagagg aggacctcca gcgtgccgtt ctcccgttcg ccggcgatgg
     1801 cgctcaccgt cgagccgatg gcgaagatgc cgacgaaggc gatggccagg gggtgctgca
     1861 gcccgagggt gagggcgccc ggcagggtga agaggctgcc cgacccgaag ttcatcaact
     1921 ccttcggaaa cactccggag tcggccaggt ccttgaacgc cgcggcgaac tggacgtaga
     1981 cgatcgggat caccatgccc cagccgagcg cggcgaccac gaccgccgcc agccgcacgc
     2041 ggttccagcg cagcgtctgg aggtagaggc gcaggttcac ccgcgctgct cctcgctcgc
     2101 gtcgtcgccc gcgtagtact cgaggaacgc ctcctcgagc cgtgccggct cgatggtcag
     2161 gtcctccagc gacggggaag cgatggcacg cacgaagccg gagacgtcgc cgagcagggt
     2221 ggcccgcagg cgatcgccct tcacctccac gtcagccagg ccggggatgc cgctgaggtc
     2281 gggggcagcg cctcgccagc gcagctcgac cttcttcttg cgacgtgcca gcagctcctc
     2341 gacgttctgg agcgccatca ggtggccgga gcggatgatc gccacgcggt cgcagactcg
     2401 ctcgacctcc gacaggacgt ggctcgagaa gaagatggtg cgccccttcg cctgctggtc
     2461 gtccatcagc gcgtagaagg cgtgctgcat gaggggatcg agcccctcgg tcggctcgtc
     2521 caggatcgac agctccgggt cgtgctggaa ggcctggatg acgccgatct tctggcgcat
     2581 gccgcgcgag tagtcgcgca ccttgcggcg caggaccgac gcgggcatct gcaggcgctc
     2641 gcagagctcc tcgcgcatgc tcgatccctg gtcctgcagc gcggccaggt actccagcgc
     2701 atcggctccg ctcaatgcgt catacagggc gatcccgccc gggaggtagc cggtccgccg
     2761 ccggatctcg accgagtccg ccacgacgtc gaggcccagc accgtcgccg agccgcgggt
     2821 cgggtgcagg aagccgagca gggtgcggat catcgtcgac tttccggcgc cgttcggacc
     2881 caggaagccg aagatttcgc cagggcgaac gtcgagcgtg aggtcgacga gcgcccgcac
     2941 atttccgtag tgcttggtca gggaatcggt ggcgatcacc ggctgcgggg tgcccatcgg
     3001 ctcggcgtcc atcggggcaa tctacaccga tgcatgccgc cgcgaggcgt tgatggtcgc
     3061 gtcggacccg gtgctacact ttgcttcccg aaccgccagg cgccgaccgc tgcggcggtt
     3121 cgtcggtgtc acccgttgaa cgccaggaac tgcccgatga agatcgctgt cccccgcgag
     3181 accgcgcccg gcgagacgcg cgtggccctg accccgcagg ccgtcgcggc actgctcgag
     3241 gacggcgtcg cggtggtcgt gcagtcgggc gccggcgacc tgtcgttcat cagcgacgag
     3301 gccttccgcg aggcgggagc caccatcgtt ccggacgctg ccgcgctcta cggcgaggcc
     3361 gatgtcgtgg tccgcgtggg acgtccctcg gacgaggaga ccggcatgct gcggaagggc
     3421 gccatcatca tcggcacgct gggaacgctc tcgaacccgg agctgggacg gcgcctggcc
     3481 gagaccggcg tgacggccat cagcatggat tccatccctc gcatcactcg cgcgcagagc
     3541 atggacacgc tctcgtcgca ggccactatt agcggttaca aggcggtcct gatcgcggcc
     3601 gaacggctcc ccaagttcat gccgctgctc acgaccgctg cggggacggt tcggccggcc
     3661 cgcggcatcg tgatgggtgc cggcgtggcg gggctgatgg cgatcggcac tgcacgacgc
     3721 ctgggcgccg tggtcgaggc gaccgacgtg cgcccggtcg tcaaggagca ggtggagagc
     3781 ctgggcggca tcttcatcga ggtcgagatg accgaggagg agaaggcaaa tgccgagacc
     3841 gccggtggct acgcccgcga gatgagcgac gactacaagg ctcgccaggc cgccctcatc
     3901 gccgagcgcg tcaaggaggc ggacttcgtg atcaccacag ccctgatccc ggggcgcccc
     3961 gcaccgcgcc tggtcaccga cgcgatgatc gcctctatga agcccggcag cgtgatcgtg
     4021 gacatggctg cggaggcggg cggcaacgtc gaggggaccg aggccggcaa ggaggtcatg
     4081 aagcacggcg tgcggatcat cggcctgacg aacctgccag ccaccatgcc cggcagcgcc
     4141 accgccatgt atgccaagaa catgcagacc ctcctcaagc acctgatcaa ggacggcgcg
     4201 atcgcgctcg acttcaccga tgagatcacc cgcggcgcga ccatcaccca cggcggcaag
     4261 gtcgtgaacg aggcgacagc caaggcgctg gggattgaac cgtccgaccc tgcaccggta
     4321 gcggctcccg gtgcgccggc agccaagtcg ggaggcgcgg catgaacgag gccgtgcaga
     4381 cgctccccac cgaggcgctg ctccaggcgc tcttcgtctt cgtcctggcc gcgttcgtcg
     4441 gctacgaggt ggtgagcaag gtgccgacca tgctccacac cccgctgatg tcaggcgcca
     4501 atgcggtcca cggcgtgatc gtcgtcggcg cgatgctcgt cgcagcctcg gctgagggcc
     4561 cgctgcggat cgtgctggcc ctgctcggcg tgatcctggg cacgatcaac gtggtcggcg
     4621 gcttcgtcgt caccgaccga atgctcgaga tgttcaagcg ccgcccggac cccaagccat
     4681 gagcgccatg gagatcgtca ccttcttcgt ctacctggtc gggtcggtca gcttcatcct
     4741 gggcctcaag ttcctctcgt ctccggtctc ggcgcggcgt ggcaatcgcc tggccgccac
     4801 cggcatggcg atcgtcatcg cctgggtctt catcacggag ttcctgctcc gcgacggcgg
     4861 cctgtcgacc gtctggatcc tgctggtggg catcccggtc ggcgggctga tcggcgccat
     4921 cggtgcgcgt cgcgtgccga tgaccgccat gccgcagatg gtcgccatct tcaacggcgc
     4981 cggcggcggt gccgcggcgc tggtggccgc ggccgagttc ctgcgcgatg ccagcgactc
     5041 ggggcccctg cttccggtca ccttcatgat cgcgaccctc cttggcgccg tcatcggctc
     5101 cgtctcgttc accggctcga tcatcgcctt cgggaagctg caggggatcg tctccgggcg
     5161 cccggtcacc taccccggcg acaaggtcgt caccggggcg gtgctggcag ggatcgtggt
     5221 cctgggcgtg gtcctcacgc tggtcaccaa cagcgtgccg ctcttcctgc tcttcgtggc
     5281 gctggcgctg gtgctgggcg tcgccgcggt catgccgatc ggcggggccg acatgccggt
     5341 cgtcgtcagc ctgctcaacg cctacaccgg catcgcggtg gcggccacgg gcttcgtgct
     5401 caacaactac gcgctgctgg tagccgggac cctggtcggc gcgtctggaa cgatcctcac
     5461 ccagctgatg tgccgggcca tgaaccggtc catcttcaac gtcctgttcg gcgccttcgg
     5521 gaccgccggc gggggcgcgg cagccggcgc gagtggcgac caggacgtgc gcgagatcag
     5581 cgccgatgac gccgcggtgc tgatggccta cggcaagaag gtgatcatcg tgccgggcta
     5641 cgggatggcg gtggcgcagg cgcagcaggc cgtgcgcgag ctgatggacc tgctcatcgc
     5701 caagggcgtc gacgtggagt tcgcgatcca cccggtcgcg ggccgcatgc ccggccacat
     5761 gaacgtcctg ctggccgagg cgaacgtccc ctacgaccgt ctcaaggaga tggacgagat
     5821 caacgacgac ttcccgaacg catcggtggc gctcgtcatc ggcgccaatg acgttaccaa
     5881 tcctgccgcc aggaccgata cctcctcgcc gatctacggc atgccgatcc tcaacgtcga
     5941 ccaggccgag cacgtgatcg tgctcaagcg ttcgatggcc tccggctttg ccggcatcga
     6001 caacccgctc tacgccgacc ccaagacgac gatgctcttc ggcgatgcca agaagagcgt
     6061 cgaggcgctg gtcaccgggg tcaaggctgc ctagcccttt ggtcgtctgg cagataccac
     6121 catcggtagt acaacagcac cgatgaggcc tcaaccgccc gccagatgtc tgtcgccact
     6181 accgtgcgtg gtaagcgctg cccgtttcgg cccgaatgac cgcttcggcg gtcactcgcg
     6241 cgctgttgca ctactccacg tggtaaccga gccatgatcg agcgctgggt cgtgctggac
     6301 gtcggcgaga cgatcatcga cgagacgcgg gtctggtcga tctgggcgga catgctcggc
     6361 attccacggc tgaccttcct ggcgggattc ggcgcggtgc tggcccgcgg cggcgagcac
     6421 cgcgacgtct tcgagctctt caacgtcgcc gattggcgca ccgcctggcc cgagcacgag
     6481 gcgatctacg ccggcttcca gccagacgac ctctaccccg atgcgatccc ggccctggac
     6541 gcgctgcgtg gccgcggcta ccgggtcgcg gtcatcggca accagccagc cagccgcgac
     6601 gaggagctgc gtcgcatcgg agtcgaggcg gaggtgatcg ccatgtcggc ggagatgggc
     6661 gtcgccaagc catcgcagga gttcttcgac cgcgtcctcg agctgcttgg ctcgcccccg
     6721 gcgtcatcgg tcgcctacgt gggggaccgg gttgacaacg acgtgctgcc ggcggtcgtc
     6781 gccgggatgc gcgccgtctg gatccgccgc gggccgtggg gcttcatcga gcggctgccc
     6841 gaaggcgtcc atcccgcgct gatggtggcg tcgctgaccg agctggcgga gcggatcgac
     6901 gaggcctggt cgggcttgcc tgaggccgag ccggacgact agactcgccg gacctccgcg
     6961 tggaggcagg gccgaggccg ggcccatatc gccggacctg aggtggagac tgcgcaacct
     7021 tgcgtggtct tcttgtcgtt ctgcaaggga accgagcgat ggagcacctg ccggcggtcc
     7081 tcttccagct cttcgtgctg ctgctggcca cgaagctcgg ggacgaggtc ttcaagcggc
     7141 tcggccagcc ggcgctggtg ggcgagatcc tgggcggggt cgtcgtcggg ccggccgtgc
     7201 tgggctggta ccagatcacg ccggagacgt cgctcttcgc ccagatcggc gcggtcctgc
     7261 tcctcttcga ggtgggcgtc catacccgca tcggggaggt cgcacgagtc ggcggcacgg
     7321 cgctatccgt cgcgctgctg ggtgtggcgc tcccgttcgt gggcggctac ctgctgggtg
     7381 gggcgctgga cctgagcgcc gccggccgca tcttcctggg cgcctcgctg accgcgacgt
     7441 cggtgggcat cagcagcggc gcgcttcgca gcttcggcgc gctcgcaacg caatcgggac
     7501 gcgtgatcct gggtgcggcg gtgatcgacg acgtgctcgc catgctcatc gtcgccgtgg
     7561 cggtgggaat ctcggatggg gacgtgagcg ccggacgcct cctgctgctg ctcgggttgg
     7621 cggcggcctt catcgggatc gtggtggtcg gaggaacggg aatcctgcgg acgcgccgct
     7681 cgctgctgac cgatccacgc ttcgccgaga ccccgttcct gcccggcatg atcctgatgc
     7741 tgggtctcgc cgccctggcg gcgcagatcg gcctggcagc gatcatcggc gccttcctgg
     7801 cagggatggt ggtcggcgag tcgagcgagc ggcacgcgct cgagaccgag acggcgccgg
     7861 tggccgcctt cttcacgccg ttcttcttcg gcttcgtggg tgcgcagatc gacccggccg
     7921 cgttcggcag cctcccgacg ctcggcctgc tcgccgcgat caccctgctc gccatcgcgt
     7981 cgaagttcgc cggctcattc ctgggggcga tgcgccgggg tcgctatcga gcggtcctga
     8041 tcgggtgggg catggtgccg cgcggcgagg tcgagatcat cgtggcaggg ctggccctga
     8101 cggcgggcgc gatcaagccg gacctctacg ccgtgatcgt gggaatggtg gtggtcaccg
     8161 ccatcgtcgt gccgccgctg atggggccgc tggtccggcg ggctgagccg gcgatcgtcc
     8221 tggaatagca gggggcggcc gcactgcctg ggcggtcaac gctcaggcga tggctgcatc
     8281 ggtcgcatca agcgcccgat cgatcaccgc gaatccttcg cgcagctgct cctcggtgat
     8341 gcacagcggc gggttggtga agacctggtt ccagtggagc atcgtgtaca ggccgtccgc
     8401 tcgcagctgg ccctcgagcg cgaccatctc cggcgatgag ccattgaacg gggccatcgg
     8461 ctccatggtg tcccgcgagc gcaccagctc gaagaccccg aacaggccga tgctccgcac
     8521 cgtcccgacg ctcggatgcc gttcctgcag gccgctgagg agctccttca tgaccgggtc
     8581 gaggcgcgcc gcgttgccga ccatgtcctc gtcgcgcagc acgcccaccg ccgcaatggc
     8641 cgccgcgcag ctgatcgggt gcgaggagta ggtgaggccg ccgaagtaga ccttctcgtt
     8701 gaagaagtcg gcgatcttgg ggctcatcgc cacggccccc agcgggaggt aggagctggt
     8761 cagccccttg gccatcgtca tcaggtccgg caccacaccc cag
//
LOCUS       DATCQR010000085         4838 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_190163, whole genome shotgun sequence.
ACCESSION   DATCQR010000085 DATCQR010000000
VERSION     DATCQR010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4838
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190163"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(245..694)
                     /gene="rplI"
                     /locus_tag="VIN32_02955"
     CDS             complement(245..694)
                     /gene="rplI"
                     /locus_tag="VIN32_02955"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEY8921570.1"
                     /translation="MKVILKRDVKGLGHAGDVKEVKNGYARNLLFPTGAAALADAGAL
                     ANWERHRGERETREQGLRAEAEATAERLRALKLEVPVKAGEKNRLFGSVTNREIADLI
                     AGEGIEVDRHDIHLRQPIKTLGDFKVDVHVMPGVSAEMTVSVVPLQG"
     gene            complement(721..1284)
                     /locus_tag="VIN32_02960"
     CDS             complement(721..1284)
                     /locus_tag="VIN32_02960"
                     /inference="COORDINATES: protein motif:HMM:NF018508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HEY8921571.1"
                     /translation="MKLSGQLRYARMARMIWKLPMYARVIWGLFRDPRTPLPLKGLLG
                     AALAYLVMPFDLIPDAIPIIGQADDLTVLMLVLDLFIANAPAEVRKEHMERAKNGTAD
                     LDKDLARLRSLMGHRYDEIRDSLPELLERYGDLRDPRAVKGFLARLRIARRGNATGSE
                     NAAADAESRDAEWQLPVAAGSGEPKLN"
     gene            1427..2110
                     /gene="lexA"
                     /locus_tag="VIN32_02965"
     CDS             1427..2110
                     /gene="lexA"
                     /locus_tag="VIN32_02965"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017727424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEY8921572.1"
                     /translation="MTRHDAERRKKILESIARIVADRGYPPSVREIAEAVGLASPSAV
                     HHHLTALERDGMVERGSHSSRALRLTVRAEAELGLPPRRPAPTERSRVTPFRMPMERD
                     RLALPIIGEIAAGQPIEAYADGAETLDVPRSLQARDDSYVLRVRGKSMIDDLIDDGDY
                     VIVQPQATAHDGDIVVALLEDNGVTLKRFYREKDRIRLQPANAEMEPIYASEVQIQGK
                     VVGVIRKLT"
     gene            2123..3328
                     /gene="dinB"
                     /locus_tag="VIN32_02970"
     CDS             2123..3328
                     /gene="dinB"
                     /locus_tag="VIN32_02970"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012524227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEY8921573.1"
                     /translation="MTRTILHADLDAFYASVEVLDDPSLRGKPVIVGGDRGTRGVVMA
                     ASYEARKFGVHSAMPIRTAASLCPTGVFLPGHPDRYRDLSAAVMRIFASYTPLVEPIS
                     LDEAFLDVTASREVFGDGEAIGRALKQRVLDEVGLVVSVGVATNKLCAKVASDLRKPD
                     ALVIVAPGDEAAFLAPLPVSRLWGVGPQSRQALSDYGVTTVGQLAALPDGTLRRRFGS
                     HGHDLALRARGIDPAPVVPWQAPKSIGHELTFDRDVDDPARLEATLLDLAESVASRLR
                     NHHMAAGAVQLKLRYEGFETLTRQVRVPHQTRESEPIYAAGVSLLRKTLVRDRAVRLI
                     GLTAINLTDVQQLTLFDAPDKTDRITRSIDAVRERFGEKAITRARLVGHRDRRRFGFG
                     EKPELPPDE"
     gene            3609..3869
                     /locus_tag="VIN32_02975"
     CDS             3609..3869
                     /locus_tag="VIN32_02975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921574.1"
                     /translation="MALTKITPTPARVQWDRRHARPSTVRVGGRQLTVTGLGAMRDET
                     AAYPADRGPRITYLVETDGGQASLIFDGRRRRWFVDALDEAA"
     gene            complement(3876..4724)
                     /locus_tag="VIN32_02980"
     CDS             complement(3876..4724)
                     /locus_tag="VIN32_02980"
                     /inference="COORDINATES: protein motif:HMM:NF012760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HEY8921575.1"
                     /translation="MKQVGWRIALITTVPPVAAALVPTLRELGHDPVAIISARRRQPA
                     PPGREPMGDETAPPGLDVLLAHDKWSVEALLRASRPDLALCWGFPWRIPQAALDVPRL
                     GSVNCHPALLPRHRGPIPLAWAFRDGDAQFGVTWHRMDSELDTGPILAQASVPMLDDD
                     YGIEVVGPRMGAVAISLLPEVFRRIAAGDPGDPQPTEGVTWAGHFGDDYATVDWSQPA
                     RFIHDQVRAWAFTFGLSPAVGPIAELDGRRMRLIRTSLTDPGGNARRVETGDGPLWIV
                     ESEPLD"
BASE COUNT          766 a         1691 c         1615 g          766 t
ORIGIN      
        1 gggagatgcg gtgacgaggc atgctccaga ccctacctgt accgaccctg gtcgaagggt
       61 agttgtccac cgacttacca acgaaacgca aacgtggctg gggatggact gtggacatcg
      121 gcgacccgcg agtgctgttc acctggcata ccagctgcgt ggatggcggc agtgaagccc
      181 ccgcccgcga cacctactct gtcccgcacg gctccagccg agcggtgcct cgccgagctc
      241 gaggtcagcc ctggaggggc accacgctga ccgtcatttc ggccgagacg cctggcatca
      301 cgtgcacgtc gaccttgaag tcgccgagtg tcttgatcgg ctggcgcagg tgaatgtcgt
      361 gccgatcgac ctcgatcccc tcgccggcga tgaggtcggc gatctcgcgg ttggtgaccg
      421 atccgaagag gcgattcttc tcccccgcct tgacggggac ctccagcttc agggcgcgca
      481 ggcgctcggc cgtggcctct gcctcggctc gcaggccctg ctcccgcgtc tcgcgctcgc
      541 cgcggtggcg ctcccagttg gcgagtgcgc cggcgtcagc gagcgcggca gcaccagtcg
      601 ggaagaggag gttgcgcgcg tagccgttct tgacctcttt cacgtctccg gcgtggccga
      661 gccccttcac gtcgcgcttg aggatcacct tcatgctgtc agtcggtccc ttccgtttgg
      721 ctagttgagc ttcggctcgc cgctgccggc cgcgaccggc agctgccatt cggcgtcgcg
      781 cgactcggcg tcggcggcgg cgttctcgct ccccgtggca ttgccgcggc gggcgatgcg
      841 cagccgcgcc aggaagccct tgaccgcacg cggatcgcgc aggtcgccgt accgctcgag
      901 cagctccggc aggctatctc ggatctcgtc gtagcggtgg cccatcagcg agcggagccg
      961 cgccaggtcc ttgtcgaggt ccgctgtgcc gttcttcgcc cgctccatgt gttccttgcg
     1021 aacctctgcc ggcgcattgg cgatgaacag gtccaggacc agcatcagga cggtgaggtc
     1081 atcggcctgg ccgatgatcg ggatggcgtc cggaatgagg tcgaagggca tcacgaggta
     1141 ggcaagcgcg gctcccagca gacccttcag ggggaggggc gtgcgcgggt cgcggaagag
     1201 gccccagatg acgcgcgcgt acatcgggag cttccagatc atgcgtgcca tgcgcgcgta
     1261 gcgcagttgg ccggatagct tcacggtgat tctccggttc cggtccaggc gggccccgat
     1321 ggtatcagag gtggcggatt tccggattcg ccacctcaaa cccgttgaca ttagaacgcc
     1381 tgttctgcta ccgtcaccga aacgtccgcc cacaaaggag ccgccggtga ccaggcatga
     1441 cgccgagcgc cgcaagaaga tcctcgagag catcgctcgt atcgtggctg accggggcta
     1501 tccaccgagc gtgcgagaga tcgccgaggc ggtcggcctc gcctcgccat cggctgtgca
     1561 ccaccacctg accgccctcg agcgtgatgg gatggtcgag cgcggctcgc attcctcacg
     1621 cgcgctccga ttgaccgtcc gggccgaggc cgagctcggt ctgcctcccc gccgaccggc
     1681 accaacggag cggagccggg tcaccccatt ccggatgccg atggagcgcg atcgcctcgc
     1741 cctcccgatc atcggcgaga tcgcagcggg ccagccgatc gaggcctacg cggacggcgc
     1801 cgagacgctc gacgtccctc gctccctgca ggcccgcgat gactcgtatg tactccgcgt
     1861 ccgcggcaag agcatgatcg atgacctcat cgacgatggt gactacgtca tcgtccagcc
     1921 ccaggcaacc gcccacgacg gcgatatcgt cgtggcgctc ctcgaggaca acggcgtcac
     1981 gctcaagcgc ttctatcgcg agaaggaccg catccgtctc cagcccgcga acgccgagat
     2041 ggagccgatc tacgcgtccg aggtgcagat ccagggcaag gttgtggggg tcatccgcaa
     2101 gctgacctag gctcctcgtg cgatgactcg caccatcctc cacgccgatc tcgacgcgtt
     2161 ctatgccagc gtcgaggtgc tcgacgaccc gtccctgcgc ggcaagccgg tcatcgtcgg
     2221 cggcgatcgt gggacccgcg gagtggtcat ggccgcgtcc tacgaggcgc gcaagttcgg
     2281 cgtccattcc gcaatgccga tccgcaccgc ggcgagcctc tgcccgaccg gcgtcttcct
     2341 gcccggtcat ccggaccgct accgcgacct gtccgccgcc gtaatgcgga tcttcgcctc
     2401 gtacacgccg ctggtcgagc cgatcagcct ggacgaggca ttcctggacg tgaccgcaag
     2461 ccgcgaggtc ttcggggatg gtgaggcgat cgggcgggcg ctcaagcagc gggtcctcga
     2521 cgaggttggc ctggtagtca gcgtgggagt ggcgaccaac aagctctgcg ccaaggtcgc
     2581 gtcagacctg cgcaagcccg atgcgctggt catcgtcgct cccggcgacg aggctgcctt
     2641 cctcgctccg cttccggtca gccggctgtg gggcgtcggg ccgcaatcgc gccaggcact
     2701 ctccgactac ggcgtgacca cggtcgggca gttagcggcg ctacccgacg ggaccctccg
     2761 ccgccgattc gggagccacg gccacgacct cgccctccgc gctcgcggca tcgacccggc
     2821 gccggtcgtt ccgtggcagg cacccaagag catcggtcat gagctgacct tcgaccgcga
     2881 cgtagacgat ccagcgcgcc tcgaggcgac cctcctcgac ctggccgaat cggtggcaag
     2941 ccgcttgcgc aaccaccaca tggcggctgg cgccgtgcag ctcaagctgc gctacgaggg
     3001 cttcgagacg ctgacccgcc aagtgcgggt gccgcaccag acacgcgagt cggagccgat
     3061 atacgccgcc ggagtctccc tgttgcgcaa gacgctggtt cgcgaccgcg ccgtgcgcct
     3121 catcggcctg accgcgatca acctgaccga tgtgcagcag ctgaccctct tcgatgcccc
     3181 ggacaagacc gatcgcatta cgcgctcaat cgacgcggtg cgcgagcggt tcggggagaa
     3241 ggcgatcact cgtgcgcgac tcgtcggcca tcgggatcgg cgccgctttg gcttcggtga
     3301 gaagcccgag ttgccgcctg atgagtagcg gatcgaacat ggccgagtcg cgtcgcgggg
     3361 actcgccctg tgacatccaa tcggaccagg cctcctgacc cggtagcatc gcgttctact
     3421 tcccggtgcg gcaacccgaa tttgggtcaa gccgacgtca ccgaacccgg ccgcgttccc
     3481 ggtagggtgc caccgcatga tccacacctc acgacccttg acaagtcgaa cacctgttcg
     3541 tatccttcgc cctaatagaa cgcctgttcg gtgcctgtgg caggcggcgt gaaggagtag
     3601 tggaaccgat ggcgctcacc aagatcacgc cgaccccggc acgagtgcag tgggaccggc
     3661 gtcatgccag gccctcgacc gttcgggtcg gtggccgaca actgactgtt accgggctcg
     3721 gcgcgatgcg cgacgagacg gccgcctacc ccgccgaccg cgggccccgc atcacctacc
     3781 tggtggagac cgacggcggc caggcctcgc tgatcttcga cggccggcga cggcgctggt
     3841 tcgtcgacgc gctggacgag gctgcctagc agccctcagt caagcggctc cgactcaacg
     3901 atccagaggg gtccgtcccc cgtctcgacc cgccgggcat tgccgcccgg gtccgtcagg
     3961 ctggtccgga tcaagcgcat gcggcggcca tccagctcag caatcgggcc gaccgccgga
     4021 gatagcccaa aggtgaaggc ccacgctctg acctggtcgt gaatgaagcg cgcgggctgt
     4081 gaccagtcaa cggtggcgta gtcgtcgccg aagtggccgg cccaggtgac cccctcggtc
     4141 ggctgcgggt cgccggggtc accggccgca atgcgcctga atacctcggg cagcaggctg
     4201 atcgccaccg cgcccatgcg cggccccacc acttcaatgc cgtagtcgtc gtcgagcatc
     4261 ggcacggagg cctgggcaag gatgggcccc gtgtcgagct cggagtccat ccgatgccag
     4321 gtcacgccga actgcgcatc cccatcgcgg aacgcccagg ccagtgggat cggcccacga
     4381 tgacgcggca gcagggcggg atggcaattg actgacccca gccggggcac gtccagggcc
     4441 gcctgcggaa tgcgccacgg gaatccccag cacagcgcca ggtccgggcg gctggcccgc
     4501 aggagcgcct cgaccgacca cttgtcgtgg gccagcagca cgtcgaggcc cggaggggca
     4561 gtctcgtcgc ccatcggctc ccgtccggga ggggccggct gccgccggcg agcgctgatg
     4621 atggcgaccg ggtcgtggcc gagctcgcgg agggtgggga cgagggccgc ggccaccggc
     4681 ggcacggtcg tgatgagcgc gatccgccag ccgacctgct tcatgcggat cggagtctag
     4741 cccgtacaat cggaggcgag gctcgcgcgc gggcgccgtg cgggtgtccg aagaccacac
     4801 gaggcagctc gccgatgggc gatccgaccc aacttacc
//
LOCUS       DATCQR010000086         4353 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_113243, whole genome shotgun sequence.
ACCESSION   DATCQR010000086 DATCQR010000000
VERSION     DATCQR010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4353
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113243"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2723
                     /locus_tag="VIN32_02985"
     CDS             <1..2723
                     /locus_tag="VIN32_02985"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921576.1"
                     /translation="ADTAVEALRADEAAGREAIAVTEAAYWSAESAARAASERREAAR
                     EALIRAEADARAIARRLADLASLIARSSDELNAVESSLQAEPVAVEPGADTKLESARE
                     AERRWRAAANAFEAADRDALLAEEALSAARASAGASIADAARRDEAVVRAAARAERIG
                     EEQRAASVASATAQAEQQAARAELRAAEAAEADAISRLEAAEAASDEARRQADEARAI
                     ASGLAERVGGLRAELGSLRERAESGSQLGSRLASAGWRPLVDAFDPPREAWAAIEALV
                     GGELREAMLWRDDGVIEHAADARGSARLLADGDASVDGRAAALAAVDARMTVAEWVGA
                     ASSPRLFARAVLAPDLATLLRGWRGLPPGWCAVTADGDLADARGLVIVRGRQDQAAGE
                     AARQHERRRELAQLVESLEGEQAKAGQAAGQALVASTQARRARDGELALRSDAGLRTS
                     RAQAVSARAAEVLHRAQQDGQSLAAELDEARTAAASGHESTPIPVDTDVATLAAAAVA
                     AQERRAELAATRDQLREAWQAAQSGADAIDFSQRGAGYQLALLEARRDQLRVALTGDR
                     ESLGRLEREGSAISKAVEAATALVAQATKERDSAGAERDRLRADLLERERARGGADTR
                     MGALERDTQAAALEAQRHEDELIAIGRERDLAIESLPPAGEDADPGTEGADSGQIGDA
                     TLEAIQADLSKVRRTLSQIGSVNPFAIEEHRELAGRLDTLTAQDADLSAASASTEELI
                     ARLEADIAEQFNAAFVAIGAKFDEFCRLLFAGGSASLQMSDGEGGEAGGIEIAVQPPG
                     KRLQRLPMLSGGERALTGVALLFAMLSVNPVPFCILDEVDAALDEANIGRFADALRLL
                     AKTIDFVVITHNRATIETADTIYGVTMTDAAVSRLLSLRLADVTAEVAVG"
     gene            2720..3634
                     /gene="ftsY"
                     /locus_tag="VIN32_02990"
     CDS             2720..3634
                     /gene="ftsY"
                     /locus_tag="VIN32_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEY8921577.1"
                     /translation="MMARPRREPTGLGLRPRPGGLGGRLRALLGRDRVVESTWDELEE
                     ALIAADVGVETTGALIAATQERVGRTASGEAVRATLAEEIRLRLDRVGSGRFELGPAP
                     SVILVVGVNGSGKTTTIAKLAARFVGDGHSVVLAAADTFRAAAIEQLRLWGEQVGVPV
                     IAQRPGADPGAVAFDALATAESRGTEVVIVDTAGRLQNKAQLMAELAKIRRVIERRLP
                     GQPRHVLLVLDATTGQNGLSQAEGFSREAGVTGIVLTKLDGTSKGGVAVAIADRLELP
                     IVFAGVGEELDSLAPFDPEAYVEWLLAA"
     gene            3742..>4353
                     /locus_tag="VIN32_02995"
     CDS             3742..>4353
                     /locus_tag="VIN32_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HEY8921578.1"
                     /translation="MFESLSARLQGVVDRLRGKAKITEADLDVALREIRLALLEADVN
                     FRVVKDFVAKVRERALGADVLTGVHPGQQVVKAVHDELVAVLGGERHVLKLDGSPSLV
                     MLVGLQGSGKTTTAAKLAVRLRRDGHKPLLVAADVYRPAAVDQLVSLGDQVGVEVHTR
                     PAGTPALEIARSGMERARQRGLDVVLLDTAGRLHVDGPMMDELV"
BASE COUNT          646 a         1445 c         1658 g          604 t
ORIGIN      
        1 ccgccgatac cgcggtggag gcactgcgtg ccgacgaggc cgccggccgg gaggcgatcg
       61 cggtgacgga ggcggcctac tggtctgcgg aatcggccgc gcgggcggcg tccgagcggc
      121 gggaggcggc gcgcgaagcc ctgatccgcg ccgaggcgga tgcccgggcg atcgcccgaa
      181 ggcttgccga cctcgcttcg ctcatcgcca ggtcttcgga tgagctcaat gccgtcgagt
      241 cctcgctgca ggcagagccg gtagccgtgg agccgggcgc cgatacgaaa ttggaaagcg
      301 cccgagaggc ggagcgccgc tggcgggccg ctgcgaacgc ctttgaggca gcggaccgcg
      361 acgccctcct ggccgaggag gcgctcagcg ctgcgcgggc aagcgccgga gcctcgatcg
      421 ccgacgccgc ccggcgcgat gaagcggtgg tgcgcgcggc cgcgagggcc gagcggatcg
      481 gcgaggagca gcgcgctgcg agcgtcgcgt cggcgaccgc acaggcagag cagcaggccg
      541 cgagggccga gctaagggcg gctgaggccg ccgaggccga tgcgatcagt cgcctcgagg
      601 ctgcggaagc agcaagtgac gaggcgcgac ggcaggcaga tgaggctcga gcaattgcat
      661 ccggcctggc ggagcgagtc ggcggcctgc gagccgagct cgggtcgctc cgggagcggg
      721 cggagagcgg gtcgcagctt ggatcgcgac tggccagcgc gggctggcgt ccgctggtgg
      781 acgccttcga ccctccccgg gaggcatggg cggcgatcga ggcgctcgtc ggaggcgagc
      841 tgcgggaggc catgctgtgg cgggacgacg gcgtgatcga gcatgcggcc gatgcccggg
      901 gatcggcgcg gctgctggcc gatggcgacg ccagcgtcga tgggcgtgcg gccgccctcg
      961 cggcggtcga cgcgcggatg accgtcgccg aatgggtcgg ggcagcctcg tcgccacggc
     1021 tcttcgcccg cgccgtgctg gccccggatc tggcgacgct gctcagggga tggcgcggcc
     1081 tgcccccagg ctggtgcgca gtgaccgcag acggcgacct ggctgacgcg cgcggcctgg
     1141 tcattgtgcg cggccgtcag gaccaggccg ccggagaggc ggcacgacag cacgaacgcc
     1201 ggcgcgaact cgcccagctc gtcgaatcgc tggaggggga gcaggccaag gccgggcagg
     1261 cggccgggca ggcattggtc gcttccacgc aggcacggcg agcgcgcgat ggcgagcttg
     1321 cgctccgatc cgacgcaggg ctgcgtacct cgcgggcgca ggccgtcagt gcccgcgccg
     1381 ccgaagtgct ccaccgcgcc caacaggatg gtcagtcatt ggcggcggag ctggacgagg
     1441 ctcgcaccgc cgccgcttcg ggccatgaat ccacaccgat acctgtcgac accgacgtcg
     1501 cgactcttgc ggcggccgcc gttgccgctc aggaacgccg cgccgagctc gccgccactc
     1561 gggatcagct tcgcgaggcc tggcaggcgg cgcagtcggg ggcggatgcc atcgatttct
     1621 cccagcgggg tgctgggtac cagctggccc tgctggaggc tcgacgagat cagctccgcg
     1681 tggcgcttac cggcgaccgg gaatcgctgg gcagactgga gcgggaaggc tcggccatca
     1741 gcaaggcggt tgaagccgcc acagcgctgg tggcccaggc cacgaaggaa cgcgattcag
     1801 ccggcgcgga gcgcgatcgg ctccgtgccg acctgctcga gcgggagcgc gcccgtgggg
     1861 gtgcggatac tcggatgggg gctcttgagc gcgataccca ggctgccgca ctcgaggcac
     1921 aacgccatga ggacgagctg atcgcgatcg gccgcgagcg ggacctggcc atcgagtcgc
     1981 tgccaccagc tggcgaggat gccgatcccg gaacggaggg cgccgacagc ggccaaatcg
     2041 gtgacgcaac cctcgaggcg atccaggccg acctgtcgaa ggtgcgtcgc acgctctccc
     2101 agattggctc cgtcaacccg ttcgccatcg aggagcaccg ggagctggcc ggtcggcttg
     2161 acacactcac tgcccaggac gcggacctgt ccgcggcgag cgcctcgacc gaggagctga
     2221 tcgctcggct ggaggccgac atcgcggagc agttcaacgc ggccttcgtg gccatcggcg
     2281 caaagttcga cgaattctgc cgcctcctgt tcgccggagg atccgcctca ctgcagatga
     2341 gcgacggcga gggaggggaa gccggcggga tcgagatcgc ggttcaaccg cccggcaagc
     2401 gactgcagcg gctgccgatg ctctccggtg gagagcgggc cttgaccggc gtggctctgc
     2461 tgtttgcgat gctgagcgtg aacccggtgc cgttctgcat cctggacgag gtggacgcag
     2521 ctctcgacga ggcgaatatc gggcggttcg cggacgctct gcgcctgctg gccaagacga
     2581 tcgacttcgt cgtgatcacc cacaaccggg ccacgatcga gacggcggac acgatctacg
     2641 gcgtgaccat gaccgatgcc gcggtctcgc ggctcctctc gcttcggctg gccgacgtga
     2701 cggcggaggt ggccgtcgga tgatggcgcg gccgaggcgt gagccgaccg gcctgggcct
     2761 gcggccacgc cctggtggcc tcggcgggcg gctccgcgcc ctgctgggtc gggacagggt
     2821 cgtcgaatcc acctgggacg agctggagga ggcgctgatc gcggccgacg tcggtgtgga
     2881 gacgaccggc gcgctgatcg ccgcgacgca ggagcgggtg gggagaacgg caagcggaga
     2941 ggccgttcgg gccaccctcg cagaagaaat ccggttgcgc ctcgatcggg ttggctccgg
     3001 caggttcgag ctcggccccg ccccgtcagt gatcctggtg gtgggggtga acggatcggg
     3061 caagacgacg accatcgcca agctggcggc gcgattcgtc ggcgacggcc acagcgtggt
     3121 ccttgccgcg gccgacacgt tccgggccgc cgcgatcgag cagctccgcc tgtggggtga
     3181 gcaggtcggc gttcccgtga tcgcacagcg accgggtgcc gatcccggcg ccgtggcctt
     3241 cgacgccctg gcgaccgccg agtcgcgggg cacggaggtg gtcattgtcg acaccgccgg
     3301 ccggctgcag aacaaggccc agctgatggc cgagctggcc aagatccggc gagtgatcga
     3361 acggcgcctg cctggccagc ctcggcacgt ccttctcgtt ctcgacgcca ccaccggtca
     3421 gaacggcctc tcgcaggcgg agggcttcag ccgcgaagcg ggagtgacgg ggatcgtgct
     3481 gaccaagctg gatggcacct cgaagggtgg cgtcgcggtt gccatcgcag atcggctcga
     3541 gctgccgatc gtcttcgcgg gggtcggcga ggagctcgac tcgctcgctc cgttcgatcc
     3601 cgaagcctac gtggagtggc tgctggcggc ctgattccaa ctcccaggag gattgccgct
     3661 ccgaggcgag ccgcctagaa tgggccaccg tctcgcccgg ccgccggctg atcgcgacac
     3721 ggaccccgta tcggttcccg catgttcgaa tcgctctccg cacgcctgca gggcgtcgtc
     3781 gatcgcctgc gcggcaaggc caagatcacc gaggccgacc tcgatgtcgc cctgcgcgag
     3841 atccgcctcg cgcttctcga agcggacgtg aacttccgcg tcgtcaagga cttcgtggca
     3901 aaggttcgcg aacgcgctct gggagcggac gtgctcaccg gcgtgcatcc cggacagcag
     3961 gtggtcaagg cggtccatga cgagctggtc gcggtcctgg gtggcgagcg ccacgtcctc
     4021 aagctcgatg gcagcccgag cctggtgatg ctggttggcc tgcagggatc gggcaagacc
     4081 acgacggcgg caaagctggc cgtgcgtctg cgtcgtgacg gacacaagcc cctcctcgtc
     4141 gcggctgacg tctaccggcc cgccgccgtc gatcagctcg tgtcgctcgg cgaccaggtc
     4201 ggcgtggagg tgcatacccg tcctgcgggg acgccggcgc tcgagatcgc ccgttcgggg
     4261 atggagcggg cccgccaacg gggcctggac gtcgtcctgc tcgacacggc ggggcgcctg
     4321 cacgtggacg ggccgatgat ggatgagctg gtg
//
LOCUS       DATCQR010000087         2627 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_128696, whole genome shotgun sequence.
ACCESSION   DATCQR010000087 DATCQR010000000
VERSION     DATCQR010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2627
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128696"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1050
                     /locus_tag="VIN32_03000"
     CDS             <1..1050
                     /locus_tag="VIN32_03000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921579.1"
                     /translation="YALTLAEEGAALADNPGTKARCLVIAGRLLHADGQLEEAERRYS
                     DARKLADESGLTTLAATWLAALRCDQGHARDALELLRLAPAASDDVDQPLITRHRELA
                     LARAHMMLGEVAQALAALDRLAADGGPVEIAQVGPDGANLRAAILVNLGEVEAADVIN
                     LKELDEARVGQLRPQLEASLIGLGESRLVAGGRRSAMRYSGEAARARVAPYPFRWQQR
                     GRSRLLQARLELAAGRVEHALAEARDLMADSSRSGDAVRSVAARLLEAEALAMSGAGI
                     DTQAIGEVLKRAGDVLGAEAWQITARLARLTGNAGWEALADRQLEHVIQGSGTHAMAL
                     RAFAQSRKEPPGSPS"
     gene            1167..1439
                     /locus_tag="VIN32_03005"
     CDS             1167..1439
                     /locus_tag="VIN32_03005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921580.1"
                     /translation="MTVEERRFPISGLVARFIDRLTDLSPGEWESVHRAISGGATQVE
                     MLEASRNAAVALAVRDLISREQFEILYAPFLLAIPIESLETPPTLD"
     gene            1488..>2627
                     /locus_tag="VIN32_03010"
     CDS             1488..>2627
                     /locus_tag="VIN32_03010"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEY8921581.1"
                     /translation="MAIIVAPQPQAAEIGLEVMRRGGNAVDAAVTCAFVQGVMDPQMC
                     GIGGCGVMLVHSPQRGDALLEFYATAGARAREDQWEKLFIREAADRYGYVLEGWVNDV
                     GYQSVGVPGTVAGLYEALTRFGTIRWDQAIAPAIPLAREGIPVSGFMHGYWTTDYGPD
                     VVPNVDRIQATPAAKAIYTKNGDLFAIGERLVQADLARTLERLATDGPDIFYKGEIAD
                     AIAADFESNGGFITKEDLAGYRVNVTEPLRGTYRGLSISAAGPPAGGLTLLQMLNFLE
                     GYDVGSHGWPSTEAARLLVEAMAWAVADRELHVADPRFVDIPTGALADKQYAAKAREA
                     VAAGATAHDRSDTTQVCAIDDQGNAVSLTHTLGSASGVVTPGLGFG"
BASE COUNT          425 a          855 c          936 g          411 t
ORIGIN      
        1 tatgccctga cgctcgcgga ggagggagct gcgctcgccg acaatccagg caccaaggcg
       61 cggtgtctcg tgatcgccgg gcgcctgcta catgccgatg ggcagctgga ggaggcggag
      121 cgccgctatt cggacgcacg caagctggcc gatgagagcg gtctgacgac gctggctgcc
      181 acctggcttg ccgccctgag gtgtgaccag ggccacgcca gggatgccct cgagctgctg
      241 cgactcgcac cggcggcttc ggatgacgtc gaccagccgc tgatcacccg tcatcgggag
      301 ctggcgctgg ctcgggcgca catgatgctg ggcgaggtcg ctcaggcgct ggcggcgctc
      361 gaccgcctgg ccgccgacgg tggcccggta gagatcgctc aggtcggccc tgatggggcc
      421 aatctgcggg ccgcgatcct tgtgaacctt ggcgaggtgg aggcggctga cgtcatcaat
      481 ctcaaggagc tggacgaggc gcgcgtcggc cagcttcgcc cgcagctgga ggcgagcttg
      541 atcggccttg gcgagagccg gctcgtcgca gggggaaggc ggtcggccat gcgttattcg
      601 ggcgaagcgg cgcgcgctcg cgtggctcca tatccgttca ggtggcagca gaggggccgg
      661 agccggctgc tgcaagccag gctggagctg gccgctggga gggtcgagca cgcgctggcg
      721 gaggccaggg acctcatggc cgactccagc cgatcggggg atgcagtccg ttcggtggcc
      781 gccaggctgc tcgaggcgga agcgctggcg atgtcgggtg cgggcatcga cacccaggcg
      841 atcggggagg tgctcaagcg ggcgggggac gtgctggggg cggaggcgtg gcagatcacg
      901 gcgcgccttg cccggctgac gggcaacgct gggtgggagg cactcgcaga tcggcagctg
      961 gagcacgtga tccaggggag cggcacgcac gccatggcgc ttcgtgcctt tgcccagtcc
     1021 cggaaggagc cccccggcag cccgtcgtaa aggcccgaaa acggctgcaa ctttgctgca
     1081 gcggtcgctg tctaagctcc agcgatgaga accccgccca agtttgctgg cacacctgtg
     1141 agagggcgct cagcagggtc ggctacgtga ccgtggaaga gcgccggttt ccgatcagcg
     1201 ggctggtggc gaggttcatc gaccggttga cggacctttc gcccggcgag tgggagtcgg
     1261 tacatcgagc gatcagtggg ggagcaaccc aggtcgagat gctggaggcg agcaggaacg
     1321 cagcggtggc gctggcggtg cgggacctca tctcccgaga gcagttcgaa atcctgtacg
     1381 cgccattcct gctcgcgatc cccatcgagt cgctggagac gccgccgacg ctcgactgaa
     1441 gccacatcgt cagggaaaac cacccgggtt tccttcattc tttcctcgtg gccatcatcg
     1501 tcgctccgca accccaagcg gccgagatcg gcctggaggt catgcggcgt ggtggcaacg
     1561 ctgtcgacgc cgccgtcacc tgcgcctttg tccagggcgt gatggatccg cagatgtgcg
     1621 gcatcggcgg ctgcggcgtg atgctcgtcc acagcccgca acgtggtgac gccctcctcg
     1681 agttctatgc gaccgccggt gctcgcgcgc gcgaggacca gtgggagaag ctcttcatcc
     1741 gcgaagcggc cgaccgctac ggctacgtgc tcgaaggctg ggtcaacgac gtcggctacc
     1801 agtccgtcgg cgtgccggga acggtagccg gcctctacga agcgctgacg cgtttcggca
     1861 cgatacggtg ggaccaggcc atcgcgccag caattccact ggcccgcgag ggcatcccgg
     1921 tcagcggctt catgcacggc tactggacga ctgattacgg ccccgatgtc gtccccaacg
     1981 tggacaggat ccaggcgaca cctgcggcga aggcgatcta cacgaagaat ggcgacctat
     2041 tcgccatcgg cgagcgcctc gtccaggcgg acctcgcacg taccctggag cgcctcgcga
     2101 ccgatggtcc cgacatcttt tacaagggcg agatcgcgga cgcgatagca gccgatttcg
     2161 aatccaacgg cggcttcatc accaaagagg atctcgccgg ctaccgcgtc aacgtgaccg
     2221 agcccctgcg cggcacctat cgcggcctga gcatctccgc cgccggcccg cctgccggcg
     2281 gactgacgct gcttcagatg ctgaacttcc tcgagggcta cgacgtcggc tcgcatgggt
     2341 ggccgagcac cgaggccgcg cgcctcctgg tggaggcgat ggcgtgggcg gtcgcggacc
     2401 gcgagcttca cgtcgccgat ccgcgcttcg tcgatatccc aaccggcgct ctggccgaca
     2461 agcaatacgc agcgaaggcc cgtgaggcgg tggcggctgg agcgacagct cacgaccgct
     2521 cggacaccac ccaggtctgc gccatcgacg accagggcaa cgccgtctcg ctcactcaca
     2581 cgctcggctc ggccagcggc gtggtgaccc ccgggcttgg gtttggc
//
LOCUS       DATCQR010000088         6490 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_5125, whole genome shotgun sequence.
ACCESSION   DATCQR010000088 DATCQR010000000
VERSION     DATCQR010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6490
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5125"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(160..840)
                     /gene="rpe"
                     /locus_tag="VIN32_03015"
     CDS             complement(160..840)
                     /gene="rpe"
                     /locus_tag="VIN32_03015"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEY8921582.1"
                     /translation="MSARPAQISASLLNADYSRLGDEVRRAVKGGVDSIHLDVMDGHF
                     VESLTIGPVVVEALRPVASLPYHAHLMVSQPLRHARAFADAGSDLIVAHVEADDDPAE
                     VIDAIHGLGRAAGLALNPETPAEAVLPYLATIDLLLVMTVHPGRGGQAFLSEVLPKLA
                     QLRDEIDRRGLALPIGVDGGVSLDTIAAAHAAGAEVMVAGSALFGEEGDLAPTVQALR
                     AAGAAVVA"
     gene            complement(837..1979)
                     /gene="rlmN"
                     /locus_tag="VIN32_03020"
     CDS             complement(837..1979)
                     /gene="rlmN"
                     /locus_tag="VIN32_03020"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007467549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEY8921583.1"
                     /translation="MTERPVAGNQPAISEVDPESLTAWLAARGEPAYRAAQVLAGAHR
                     PTVSSLDDLTDLPRPLRGAVADAFRFSSIVGSHTLVADGGLTAKAVHELVDGQRIESV
                     LMRYPGRGTSLGRTTICISSQAGCAVGCPFCATGQAGFGRQLSAGEIVDQVLHWHRDP
                     WLALGADWRPGGGRSHYNIVFMGMGEPLNNVDRVFASVRLLNDPARLGIGARHITVST
                     SGVVPGIDRMIDELPQVNLAISLHAASDDLRDELVPINRKWPIAEVVAAGQRFAARTR
                     RRVSLEYVLIDGVNDAPAQATGMAALASGWLCHVNLIPLNPTPGSRWSGTDAAGVRRF
                     AATIERAGVPVTVRDTRGREIEAACGQLHAQLAGRAIGTAQPMVPA"
     gene            complement(1972..2718)
                     /locus_tag="VIN32_03025"
     CDS             complement(1972..2718)
                     /locus_tag="VIN32_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010024599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HEY8921584.1"
                     /translation="MAGFLPYILLVMLPSIAITGWAAWRVRSTYARWSKVDSGIDLSA
                     FDFARRLLDRQGLTEVKIEPVPGQLTDHYDPRGKVLRVSSAVAGQQQLGSYDSTDRSV
                     GFPQGSGTLSVAAAAVIAHEVGHALQDREHDPWMAVRQTIVPAAQLGSGLAPWLIIAG
                     VWFSIMQLAWVGLIFFGAAVIFTFVTLPVEIGASGKALAFVSSLGITGERQVGAREVL
                     KAAAWTYVAAALAALLTFLYYLMLILGRRD"
     gene            complement(2802..3740)
                     /locus_tag="VIN32_03030"
     CDS             complement(2802..3740)
                     /locus_tag="VIN32_03030"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF012760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEY8921585.1"
                     /translation="MGRIAFLGTGLFGVPLLSRLPPLCDELIVISQPDKPAGRGLQTR
                     PSPVVAWAREHGIAVETPSRLRSDEGRTLLRRFAPDGLLLAAYGQLVPSDLLDLASRP
                     PLNVHPSLLPRHRGAAPVASTILAGDSRGGVSLMVMTAELDAGPIVAQWPATLTGRET
                     TPELEGRLAQLAAEVVPDELDGWVAGEVVTTPQDESLATMIRPFRRSDGWIDWRESAT
                     IVDRRVRALQPWPGAWTTLDGRRLHIRAAHPVAGVDGPLVGTLLPGEWPLVACGQGAL
                     ALELVQPEGRPVMPADAWRRGMPREHVLLGGGTPPA"
     gene            complement(3749..4255)
                     /gene="def"
                     /locus_tag="VIN32_03035"
     CDS             complement(3749..4255)
                     /gene="def"
                     /locus_tag="VIN32_03035"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEY8921586.1"
                     /translation="MVNRPILTADEPILHQRAKKVTHFDASLHRLLQEMVETMRDAPG
                     IGLAANQIGVPLQVAVIELEERVTELVNPQIVRANGEVIDWEGCLSVPGFVAEVRRHA
                     KVTVKARDRHGKEFRVKGEDLFARALQHEIDHLNGVLYIDYLDSLEELVRVSEHPSEV
                     EEPTTAGI"
     gene            complement(4310..>6490)
                     /gene="priA"
                     /locus_tag="VIN32_03040"
     CDS             complement(4310..>6490)
                     /gene="priA"
                     /locus_tag="VIN32_03040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEY8921587.1"
                     /translation="PEPGIELRSVEAVVSAPMLTPDLLALAEEIAVYYCAPLGGTIGA
                     MLPPGLESRLERRWTTPDASLLPDPLRAMLSGPDGISDAMLKRSLGPRWSPVADRLRR
                     SGALVARWSLRPPELKARRVRTLRRLPDADAPVRTAPLQRAILDALGSEARSLPELAE
                     EIGVEPASAMAAARRLVARGAAAMDWQVVERDPRAHRPTEVARDHLLAAEQQAAIAQL
                     EALAPGGELLLEGVAAAGKTDVYLAALAGVLTEGGTAIVLVPEVSLVTQLADRVASLA
                     GDQLAVMHSGLSAGERHDTWWRIMRGEARVVVGTRMAVFAPLSDLRLIILDEAHDGAY
                     KADRAPRYDARWVARRRGGLTGARVMMGTATPDVVTVARVRGGLVDRARLLERRVGRT
                     PAVEVVDLRAELAEGNRSIFSRALTAALSDLRQGSEQAILLMNRRGAATFVLCRDCGE
                     SLRCPDCDLPFVFHLSGEQLRCHHCGRTARPPEKCPHCGSSRIRYFGAGTQRVEAELR
                     TRFPHLRVSRLDSDALAARRGFEAIYDDFRDGRVDVLVGTQLAAKGLDLPSVTLAAVI
                     AADVTLNLPDYLAPERTFQLLAQVAGRAGRGEQPGRVLIQTYAAGHYAIRAAARLDVD
                     GFADEELPRRRLLGYPPSSVLARLLVADPDRARAESRGRDAAAAVAFPGVEVHGPLPS
                     YVPRRAGRWRFQVVIRAQDQAARAAALERVPPGVAIDVDPESLL"
BASE COUNT         1008 a         2328 c         2272 g          882 t
ORIGIN      
        1 tcggcacagg tgcctggttg gtccgatcca ccaccgtgac ggtgacgtca cgcggcggcc
       61 gcggggtcgg gcttgcctcg gggcccgggg cggagcagcc gatcacgatc accgcggtca
      121 gcagcgcgac gaccaggggc cggcagcgca ggaactgggt cacgccacca ccgcggcccc
      181 ggcggcgcga agcgcctgga cggtcggcgc caggtcgccc tcctcgccaa acagcgccga
      241 tccggcgacc atgacctcgg cccctgcggc atgcgccgcc gcgatcgtgt caaggctcac
      301 gcccccgtcc acgccgatcg gcagcgcaag tccgcgacgg tcgatctcgt cacgcagctg
      361 ggccagcttg ggcagcacct cggacaggaa tgcctgcccg ccgcgaccgg gatggaccgt
      421 catgaccagc agcaggtcga tcgtggcgag gtatggtagg accgcctccg cgggagtctc
      481 cggattgagc gccagcccag cagcccggcc gaggccgtgg atcgcatcga tcacctcggc
      541 aggatcgtca tcggcttcga catgggccac gatcaggtca ctcccggcgt cggcgaaggc
      601 gcgtgcatgg cggagcggct gcgagaccat gaggtgggcg tgatacggaa gcgatgccac
      661 cggtcgcagc gcctcgacca cgaccgggcc gattgtcaag ctctctacga agtggccatc
      721 catcacgtca aggtggatcg agtcgacccc acctttgacg gcgcgccgca cctcgtcgcc
      781 aagccgggag tagtcggcat tcagcagact ggcgctgatc tgtgccgggc gtgcgctcac
      841 gcagggacca tcggctgtgc cgtcccgatc gcccgtccgg cgagctgggc atggagctgg
      901 ccacacgccg cctcgatctc tcgcccgcgc gtgtcgcgca cggtgacggg cacgccagcc
      961 cgctcgatcg tggccgcgaa gcggcgaacc cccgccgcgt cggttccgct ccagcgcgat
     1021 cccggcgtcg ggttcagcgg gatcaggttg acgtggcaga gccacccgct ggcgagtgcg
     1081 gccatgccgg tcgcctgggc cggagcgtcg ttcaccccat cgattagcac gtactcgagg
     1141 ctgacgcggc gcctggtgcg cgccgcgaag cgctggcctg ccgccaccac ctcggcgatc
     1201 ggccacttgc ggttgatcgg caccagctcg tcgcgcaggt cgtcgctggc agcgtgcagg
     1261 ctgatagcaa ggttgacctg tggcagctcg tcgatcatgc ggtcgatgcc ggggaccacc
     1321 cccgaggtgc tgaccgtgat gtgccgcgct ccgatcccca gccgggccgg atcgttcagc
     1381 agacggacag aggcgaagac ccggtcgacg ttgttcagcg gttcgcccat ccccatgaag
     1441 acgatgttgt agtggctccg accacccccg ggtcgccaat ccgcgccaag ggcgagccag
     1501 ggatcgcgat gccagtgcag cacctggtcg acgatctccc cagcgctgag ctggcggccg
     1561 aagccggcct gcccagtcgc acagaaggga cagcccaccg cacagccagc ttgcgagctg
     1621 atgcagatgg tggtgcgccc gaggctcgtg ccgcgacccg ggtagcgcat cagcaccgac
     1681 tcgatgcgct ggccgtctac cagctcgtgc accgccttgg cggtcagccc tccatcggcg
     1741 acgagcgtgt ggctgccgac gatggacgag aagcggaatg catccgccac ggctccccgc
     1801 agtggacgcg gcaggtcggt caggtcgtcg aggctggaga cggtcggccg gtgggcgccg
     1861 gcgagcacct gggcagctcg atacgcgggc tcaccgcggg cggcgagcca cgcggtgagc
     1921 gattcaggat cgacctcgct gatcgccggc tggttcccgg cgactggacg ttcagtcacg
     1981 ccgccccagg atcagcatca ggtagtacag gaaggtcagc agcgcggcca gcgccgcggc
     2041 gacgtaggtc caggcggctg ccttcagcac ctcgcgggcg ccgacctgtc gctccccggt
     2101 gatccccagg ctgctgacga aggcgagagc cttccccgag gcgccgatct caaccgggag
     2161 cgtcacgaag gtgaagatga cggcggcccc aaagaagatc aggccgaccc aggcgagctg
     2221 catgatgctg aaccagaccc cggcgatgat cagccacggc gcgagccctg acccgagctg
     2281 cgcggcgggc acgatcgtct ggcgaactgc catccacggg tcatgctctc gatcctggag
     2341 ggcgtgaccg acctcgtgcg cgatcaccgc ggcggcggca accgagaggg tgccggagcc
     2401 ctgcggaaac ccgaccgagc ggtcggtcga gtcgtaggag ccgagctgct gttgcccggc
     2461 caccgcggag ctgacgcgaa gcaccttgcc gcgcgggtcg tagtggtcgg tcagctggcc
     2521 ggggaccggc tcgatcttga cctcggtaag gccctgccga tcaagaagcc ggcgggcaaa
     2581 gtcaaaggct gaaaggtcaa tcccggagtc gacctttgac caccgggcgt aggttgagcg
     2641 aacacgccac gccgcccaac cggtgatggc gatgctcgga agcatgacca gcaagatgta
     2701 cggcaggaaa ccggccatgg tcttgttgct acctccttgg aagcgcggcg agtatagccg
     2761 agcctcccct tcgcctcagt gaaggcgagg tgaagatggg actatgcggg aggcgttccg
     2821 ccgccgagca ggacgtgctc gcgtggcatt ccacgacgcc acgcgtcggc cggcatgacc
     2881 ggccttccct caggctggac gagctcgagc gcgagcgccc cctgcccaca ggccaccagc
     2941 ggccactcac cggggagcag agtgccgacc agcggtccat caacccccgc cacagggtgg
     3001 gcggcccgga tatggagccg ccgcccgtcg agcgtcgtcc aggcgccggg ccatggctgg
     3061 agggcgcgga cgcggcggtc gacgatcgtg gcgctctctc gccagtcgat ccagccgtcg
     3121 ctgcgccgga agggtcgaat catggtggcc aggctctcgt cctgcggcgt tgtgaccacc
     3181 tcacccgcga cccagccgtc gagctcgtcc gggaccacct ccgcggccag ctgagccagg
     3241 cgtccctcga gctcgggggt cgtctcgcga ccggtgagcg tcgccggcca ttgggcgacg
     3301 atcggcccgg cgtccagctc ggcggtcatc accatcagcg acaccccgcc ccgcgagtcg
     3361 ccggccagga ttgtcgaggc caccggcgca gcgccccgat gccggggcag cagcgagggg
     3421 tgcacgttaa ggggcggacg cgacgcgagg tccagcaggt cggatggcac cagctgcccg
     3481 tacgccgcga gcaggagccc gtcaggcgcg aagcggcgaa ggagggttct cccctcgtcg
     3541 gagcgaagtc ggctgggtgt ctcgaccgca atgccgtgtt cccgcgccca ggcgactacg
     3601 ggtgacggac gggtctgcag gccgcgcccg gccggcttgt ccggctggct gatgacgatc
     3661 agctcatcgc acagcggcgg gagacgcgag aggagcggga cgccgaacag gccggtgccg
     3721 aggaacgcga tgcggcccaa ttgcggcact agatccccgc ggtcgttggt tcctccacct
     3781 cgctcgggtg ctcgctgacg cgaaccagct cttcgagcga atcgaggtag tcgatgtaca
     3841 ggaccccgtt caggtgatcg atctcgtgct ggagcgcgcg cgcgaacagg tcctcgccct
     3901 tgacgcggaa ctccttgccg tgccgatcgc gcgccttgac cgtgaccttc gcgtggcggc
     3961 gaacctcggc cacgaagccc ggcacggaga ggcatccttc ccagtcgatc acctcgccgt
     4021 tggcgcgcac gatctgcggg ttgacgagct ccgtcaccct ctcctcgagc tcgatcaccg
     4081 ccacctgcag cggcaccccg atctggttgg cggccaggcc gatccccggc gcgtcgcgca
     4141 tcgtctcgac catctcctgc agcaggcggt gcagcgacgc atcgaagtgc gtcaccttct
     4201 tggcgcgctg gtgcaggatc ggctcatcgg cggtgaggat cggacggttg accatcgccc
     4261 gcattgtacc gatccacgga tcccgtccag accacgcgga cgactcccgc tacagcaggg
     4321 attcagggtc gacgtcgatc gccacgccgg gcgggacgcg ctccagcgcc gcagccctcg
     4381 cggcctggtc ctgggcacga atcaccacct ggaaccgcca tcggcccgct cgccgaggga
     4441 cgtacgaggg gagcggcccg tgcacctcca ccccggggaa tgcaaccgcg gctgcggcgt
     4501 ctcggccgcg tgactcggcc cgggcgcgat cggggtcggc aaccagcagg cgggcgagca
     4561 cgctcgatgg cgggtagccg agcagccggc gacgcgggag ctcctcgtcg gcgaagccgt
     4621 cgacgtccag gcgtgcggcc gcgcggatgg cgtagtgacc cgccgcatag gtctggatca
     4681 ggacccgtcc gggctgctct cccctccccg cgcgccccgc cacctgggcc aggagctgaa
     4741 aggtgcgctc cggcgcgaga tagtcgggca gattgagtgt cacgtcggcg gcgatcaccg
     4801 ccgccagcgt cacgctcggg aggtcgagcc ccttggcggc cagctgggtc cccaccagga
     4861 cgtcgacgcg gccgtcgcgg aaatcgtcgt agatcgcctc gaagccgcgc cgagcggcaa
     4921 gggcatcgga gtcgaggcgt gacacccgca gatggggaaa gcgcgtgcgc agctcggcct
     4981 cgacccgctg cgtcccggcc ccgaagtagc ggatccggct gctcccgcag tgcgggcact
     5041 tctccggggg cctggccgtc cgtccgcaat ggtggcagcg cagctgttcc ccgctcaggt
     5101 ggaagacgaa cggcaggtcg cagtcggggc agcgcaggct ctcgccacag tcgcggcaga
     5161 gcacgaaggt cgctgccccg cgacggttca tgagcaggat cgcctgctcg ctcccctgtc
     5221 gcaggtctga cagcgcggcc gtcaacgctc gagagaagat cgagcgattc ccctccgcca
     5281 gctctgctcg cagatccacg acctcgactg ccggtgtgcg gcccacgcgg cgctccagca
     5341 ggcgggcccg atcgaccagc cctccgcgaa cgcgcgccac ggtgaccacg tcgggcgttg
     5401 ccgtccccat catcacgcgc gcccccgtca gcccgccgcg ccgccgagcg acccaccggg
     5461 cgtcgtaacg gggagcgcga tcggccttgt acgcgccgtc atgcgcctcg tccaggatga
     5521 tcagccgcag gtcggagagg ggtgcgaaga ccgccatgcg tgtgcccacg accacgcgcg
     5581 cctcacctcg catgatccgc caccacgtgt cgtgacgctc ccccgccgac agcccggagt
     5641 gcatcaccgc cagctggtct ccggccagcg aggcaacgcg gtccgccagc tgggtcacca
     5701 gcgagacctc gggcacgagc acgatcgccg tgcccccctc ggtcagcacc cccgccagcg
     5761 cggcgaggta cacgtcggtc tttccggcgg cggcgacccc ctccagcagc agctcaccgc
     5821 cgggagcaag cgcctccagc tgggcgatgg cggcctgctg ctcggcggcc aggaggtggt
     5881 cgcgcgcgac ttcggtcggc cgatgggcgc gcgggtcgcg ctcgacgacc tgccaatcca
     5941 tggcggcagc gccgcgggcc accaggcgac gagccgccgc catcgctgat gccggttcga
     6001 caccgatctc ctcggccagc tccggcaggc ttcgcgcctc gctgcccagg gcatcgagga
     6061 tcgcgcgctg aagcggcgcg gtgcggactg gcgcgtccgc gtcgggcagt cggcgcaggg
     6121 tccgcacgcg acgcgccttc agctccggcg gtcgcagcga ccagcgcgcg accagcgccc
     6181 cggagcgccg cagccggtcg gcgacggggc tccagcgcgg gccgagcgag cgcttcaaca
     6241 tagcgtcgct gatcccatcg ggacccgaca gcatcgcacg cagcgggtcc ggcagcaggc
     6301 tggcatccgg cgtcgtccag cgtcgctcca ggcgcgattc gaggcctggc gggagcatcg
     6361 cgccgatggt cccgccgagc ggcgcgcagt agtagacggc gatctcctcg gccagggcga
     6421 gcagatcggg ggtcagcatc ggcgccgaga cgaccgcctc gaccgatcgc agctcgatcc
     6481 ccggctcggg
//
LOCUS       DATCQR010000089         9301 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_175365, whole genome shotgun sequence.
ACCESSION   DATCQR010000089 DATCQR010000000
VERSION     DATCQR010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 9301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9301
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175365"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..592
                     /locus_tag="VIN32_03045"
     CDS             <1..592
                     /locus_tag="VIN32_03045"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8921588.1"
                     /translation="NAGADPMTVLPIGSEAAPDVVFFAGYTNTGGAQLRLAMGEGGLE
                     AVPFLSWDGLYDGSGTDDGSFIQQVGVAAVGSYIAHASLAPPKASFVDAYRSAYQTEP
                     DEYAGAAYACVEVIVAGLRAIAASSPSVDGLREALRAYAVDPTHRYETVLGTIGFDAN
                     GDSIQQFVTFYRADPSAEGGAGDWVIEKQQDFGPAL"
     gene            complement(589..918)
                     /locus_tag="VIN32_03050"
     CDS             complement(589..918)
                     /locus_tag="VIN32_03050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921589.1"
                     /translation="MQTIGIVLNVGVDEVEAFEGGFREMEAPIWADLHGRGLLTMATL
                     TRLDISTLKVDGAAQYLVVAIFATDEGHHEHDNDARFEAWNKRADAYQIEEPFVFGGD
                     TIVSQGP"
     gene            1056..1301
                     /locus_tag="VIN32_03055"
     CDS             1056..1301
                     /locus_tag="VIN32_03055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921590.1"
                     /translation="MKHSSLWVLMVGSLGAGVLMVFGGAAAGSDIGQLAAGYGLLVVG
                     SSLFLGFGLAIQALVLKAVARPRSEVRSGGTILGHRV"
     gene            complement(1298..2395)
                     /locus_tag="VIN32_03060"
     CDS             complement(1298..2395)
                     /locus_tag="VIN32_03060"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEY8921591.1"
                     /translation="MTGFACQAISPTQSRPRCSEPPRRPGKGQPTDADPVADAPMVDV
                     TADAIRSAHGRIPSVFRDSPQFVSEPLSEAIGVAVVVKLETANPIGCFKGRGTWLALS
                     ELVAAGRVGERLGVVVASAGNFGQGVAYAGRAMGIPVSVVAATTASPMKLAAMRRLGA
                     DVRTVGEDFDAARLAAADLAADSGWQLLVDGEDPWISIGAGTIALELSNAVVSGDLPA
                     LDRFYVPVGNGALIAGIGTWLRTEAPATRVIGVQAAGAPAMTLSWRERRPIETQRADT
                     IADGIAARVPVRAALELMIEVVDEMLLVGDDQIVAATHEMSAAVPTAVEPAAGAAWAA
                     VRAAQSHTGVIGVLITGGNVTSRGASGGGAA"
     gene            complement(2283..3599)
                     /gene="hflX"
                     /locus_tag="VIN32_03065"
     CDS             complement(2283..3599)
                     /gene="hflX"
                     /locus_tag="VIN32_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEY8921592.1"
                     /translation="MPTHPSFTDLTAPEERAFLIGLDDPGDGRWPIERSLAELAALAQ
                     TAGAVVVGSASQRRTHPDPASYFGKGRAAELVDERAATGFNLLIVDDELAPNQQRALE
                     KLLDCKVLDRSALIIDIFARHATTREGRLQVELAALEYHLPRLTRLWTHLSRTGGGIG
                     TRGPGESQLETDRRLIRDKIRKVRSELEDVKRQRATAARQRERSEVATVALVGYTNAG
                     KSTLLNRLATADLFVADMLFATLDPTSRRVTLPSGQRIVVTDTVGFINKLPHDLVEAF
                     RATLEEVLRADLLIEVVDASDPDFVGQQEAVQVVLDELGAGDKPRIVAFNKIDLLAPG
                     LRAASMPAGDRAVFVSAATGEGIEPLLARVGGLLRDQMVAVDAVVPWSRGELVARAKV
                     AGDVAERFTDDGVRLSGHLPNAIAAEVQRAAPEARQGAAHRRRSRR"
     gene            3737..5380
                     /locus_tag="VIN32_03070"
     CDS             3737..5380
                     /locus_tag="VIN32_03070"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY8921593.1"
                     /translation="MTDASVQPDGSTKRGVPSTASMAVRMLIPLTAGAAASIAAETLL
                     SPTLPNSLVRAATSIGIGACLSAFGLAIMLIRPLTRSRADLQVRYQEAVADALLDPLT
                     GLGNHRAFQEELDSQIENATRYEVPVALVLIDLDEFKQINDSAGHAVGDQTLASFGRL
                     VGSVLRKVDRPFRIGGDEFAILLPHTDADAAHIVARRLLVSALQPNVRDPKIKPLSFS
                     AGISSLPGPATSRTQLYTQADTALHAAKHAGRTEVLVFDPGAEVAATAASTSAAIAEV
                     IEQKLLRPVFQPIVNLATGGTLGYEGLIRPVPPAPYADPASLFAAAEASGHVVPLDLA
                     CVEAIVSAAASLPKDLFLSVNMSPRTIEAPEFSAPAMLNILARYDFPPERLIIELTEH
                     QPIADLERVRHKLDTCRSAGMRMAADDLGSGNSGLRLLSDLRFDIVKVDLGLVQRSST
                     GARSSAVVESIVAFASRMGALVIGEGVEHEEQVEQLTQLGVTAAQGYLFSRPGPLPDW
                     AGEKRLKRAADRQTPAAVVDEDPDLESWRRKIGLATPAA"
     gene            complement(5377..6318)
                     /gene="miaA"
                     /locus_tag="VIN32_03075"
     CDS             complement(5377..6318)
                     /gene="miaA"
                     /locus_tag="VIN32_03075"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEY8921594.1"
                     /translation="MGPIAGIVGPTGSGKTELSLALAARIPIEILVADSRQVYRGMDI
                     GTAKPDGRARDAVPHHLIDLVTPGQAFSVASWADAARRLVTEVTARRRLPLVVAGSGL
                     YLAALLDGYAFGPAPRAEDRARLNHELATAGVGVLADRLRTVDPSGAARTDLRNPRRV
                     ARALERAASRAGTDASALAAKPWEGPISLVGISRPIEVLNRRIDERAQWLFANGLVEE
                     VRGLLEAGHDPRQAPLTSHGYGEAAHYLAGEWSLEEAVAVTVRRTRQYAKRQRTWFRR
                     DERIVWLSAGEAPADDPTLVAAAERVLERLPRARVEA"
     gene            complement(6319..7128)
                     /locus_tag="VIN32_03080"
     CDS             complement(6319..7128)
                     /locus_tag="VIN32_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008413382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HEY8921595.1"
                     /translation="MTDRTALLRCMVIGDIIGKPGRLAVVGSLRHLRTELDLDLVVAN
                     GENLAAGAGLTPSLAEELFANGVDVITSGNHIWDKREIYDYLDSGRPVLRPLNYPDDA
                     PGKGWLLHTLPDGDRVAVVNVMGRVFMNQLDSPFAAMDGLLDGAAEPLPLVRIVDFHC
                     EITSEKNAMGWYLDGRVTAVLGTHTHVPTADARLLPKGTAYISDVGMTGPRNSVIGFS
                     LETVLPRFLTHLPTRFAVAEGPVAFNAVLIEAERGTGRATSITQLQRVIEV"
     gene            complement(7125..8654)
                     /gene="rny"
                     /locus_tag="VIN32_03085"
     CDS             complement(7125..8654)
                     /gene="rny"
                     /locus_tag="VIN32_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018035138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEY8921596.1"
                     /translation="MPESNVVVAAGAALIVGIAIGYLSRRFLAAGSVKHAEGYAERLM
                     AEARAKQKEIVLEGKDDALKVQRAAEEEAREKRADLQRQERLLLDRAEGLDRKVESLE
                     RREVAFEERQRDVDAEKARLVELQQQQLLELERVSGLTASEARQSLIQLIVDEAQAEA
                     HQKVREIERSAVEEGEEHARRVLTTVMQRIAADHTSEATVTVVPLPSEEMKGRIIGRE
                     GRNIRALEQATGVDLIIDDTPEAVVLSGFDPVRREVARMALTKLISDGRIHPGRIEET
                     VAKCRAEIEVVMRQAGEQAAYDAGVPGLHPELIKLLGRLKYRTSYGQNVLNHCVETAR
                     LSGLLAAEIGANIPESKKGGLLHDIGKAIDHEVDGPHALIGGDIAKRYGVNAVVCNCI
                     AAHHQEVEQESTEATVVQIADAISASRPGARGESLDNYVKRLDDLQQIALAFPGVERC
                     YAIQAGREIRILVRPEEMDDMASSRLARDVVKKIEEQLQYPGQIKVTVIRETRAVDYA
                     R"
     gene            complement(8811..>9301)
                     /locus_tag="VIN32_03090"
     CDS             complement(8811..>9301)
                     /locus_tag="VIN32_03090"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY8921597.1"
                     /translation="RNAHLRPRPTFSGKRHPHLVLGGDGGPRLARLVATYADEFNRQS
                     ATPDRLREAYGRIATACAEAGRDPETVVRSAMVGVLVAETESDLRERVQQQIAMLGGG
                     GADAETWLRERTGRWIIGTPDAARQVIESLEAAGAQRIMLQDFLPRDLEMVTLLGRIA
                     AG"
BASE COUNT         1368 a         3308 c         3105 g         1520 t
ORIGIN      
        1 gaatgcgggg gcagatccga tgaccgtcct cccgattggc agcgaggcgg caccggatgt
       61 cgtctttttc gcgggataca ccaacaccgg cggcgcgcag ctgcgcctgg cgatggggga
      121 gggcggcctc gaggcggttc cgtttctcag ctgggacggc ctctacgacg ggagcggcac
      181 cgacgacggc tcgttcatcc agcaggtggg ggtggccgcc gtcggctcat acatcgcgca
      241 cgcgtcccta gcaccgccca aggcaagctt cgtcgatgcc taccgttccg cctaccagac
      301 ggagccggac gaatacgcag gggcggccta cgcctgtgtc gaggtcatcg ttgccgggct
      361 ccgcgccatc gccgcgagca gccccagcgt ggatgggctg cgcgaggcgc ttcgggccta
      421 cgcggtggac ccgacgcatc gatacgagac ggtgctgggc accatcggct tcgacgcaaa
      481 cggcgactcg attcagcagt tcgtgacgtt ctatcgcgcc gacccgtccg ccgaaggagg
      541 tgcgggcgac tgggtgatcg agaagcagca ggacttcggc cccgcccttt agggaccctg
      601 cgagacgatc gtgtcgccgc cgaagacgaa gggctcctcg atctggtacg catcggcgcg
      661 cttgttccaa gcctcgaatc gtgcgtcgtt gtcgtgctcg tgatggccct catcggtggc
      721 gaagatggcg accaccaggt actgcgcagc tccatccacc ttcagggtgc tgatgtcgag
      781 ccgcgtgagg gtcgccatcg tcagcaggcc gcgtccgtgc agatcggccc agatcggcgc
      841 ctccatctcc cgaaacccac cctcgaacgc ctcaacctcg tccacgccga cgttcagaac
      901 gattccgatg gtctgcatcc ctgcctcctc gctgcctttt ctggatcaag gtcacataac
      961 atctctttgc ggaagcctag tggtaggacc aatggccgga atcatgcacc ggttggtgga
     1021 tactcgaccc tcgagcgcgg acctacgctg ggcacatgaa acactcatcg ctctgggtcc
     1081 tgatggtcgg cagcctcggc gccggcgtgc tgatggtctt cggcggcgcg gccgcaggaa
     1141 gtgacatcgg ccagctggcg gcaggatatg gactgctggt cgtcggcagc tccctcttcc
     1201 tcggattcgg cctggcgatc caggctctgg tcctgaaggc tgtggctcgg ccacgcagcg
     1261 aagtccgctc aggcggcacg atcctcggac accgcgtcta ggccgctccg ccgcccgatg
     1321 cgccgcggga ggtcacgttt cctcccgtaa tcagcacgcc gatgactccc gtgtgcgatt
     1381 gagccgcccg aaccgctgcc caggcagccc cggctgccgg ctccaccgcg gtcggcacgg
     1441 cggcagacat ctcatgcgtc gccgccacga tctgatcgtc gccgaccagg agcatctcgt
     1501 cgaccacctc gatcatgagc tccagcgccg cgcgcaccgg cacccgggcg gcgatcccat
     1561 ccgcaatcgt gtcggctcgc tgggtctcga tcgggcgccg ttcgcgccat gacagcgtca
     1621 tggcaggcgc gccggcagcc tgcaccccga tgacgcgggt ggctggcgcc tctgtccgaa
     1681 gccatgtgcc gatgccggca atcagggcac cgttgccgac cggcacgtag aaccgatcga
     1741 gcgccggcag gtcgccgctc acaaccgcgt tgctgagctc cagcgcgatc gtgcccgccc
     1801 cgatcgagat ccaggggtct tccccgtcta ccagcagctg ccagccactg tcggccgcca
     1861 ggtcggcagc cgcgaggcgc gcagcgtcga agtcctcccc caccgttcgc acgtcggcac
     1921 cgaggcgtcg catggcggcc aacttcatcg gacttgcagt cgtcgcagcc accacgctga
     1981 cgggaatgcc catcgcccga ccggcgtagg cgaccccctg tccgaagttc ccggccgaag
     2041 cgacaaccac ccccaggcgc tcccccaccc tgcccgccgc cacgagctcg gagagagcga
     2101 gccaggtccc gcgacccttg aagcacccga tcgggttggc tgtctcgagc ttgacgacga
     2161 ccgcgacccc aatggcctcg gacaacggct cggagacgaa ctggggcgaa tcccgaaata
     2221 cgctcgggat gcgcccgtga gcggaccgaa tcgcatcggc cgtaacgtcg accatcggcg
     2281 cgtcagcgac gggatcggcg tcggtgggct gccccttgcc gggcctccgg ggcggctcgc
     2341 tgcacctcgg ccgcgattgc gttggggaga tggcctgaca ggcgaacccc gtcatcggta
     2401 aatcgctcag ccacgtcgcc ggcgaccttg gcgcgagcaa ccagctctcc gcgagaccag
     2461 ggcaccaccg cgtcgaccgc caccatctgg tcgcgcaaca gaccgccgac ccgcgccagc
     2521 agaggctcga tcccctcgcc cgtggcggcg ctgacaaaga cggcccggtc gcccgccggc
     2581 atcgaggcgg cacgaaggcc aggcgccagc aggtcgatct tgttgaacgc cacgatgcgc
     2641 ggcttgtccc ccgctccgag ctcgtccagg accacctgga cggcctcctg ctgtcccacg
     2701 aagtccgggt ccgaggcgtc caccacctca atcagcaggt cggctcgaag cacctcctcg
     2761 agagtggcgc ggaaggcctc gaccaggtcg tgcggcagct tgttgatgaa gccgaccgtg
     2821 tcggtcacga cgatccgctg gccagagggc agcgtcacgc gacggctggt cgggtcaagc
     2881 gtggcgaaca gcatgtccgc gacgaacagg tccgccgtgg ccaatcggtt cagcagggtg
     2941 ctcttgccgg cgttcgtata gccgaccaga gcgactgtgg caacctcgga ccgctcgcgc
     3001 tgccgtgccg cggtggcacg ctggcgcttc acgtcttcca gctcggagcg gaccttgcgg
     3061 atcttgtccc ggatcagccg tcggtcggtc tcgagctggc tctcgcctgg gccgcgggtg
     3121 ccgatcccgc ctcccgtgcg cgacaggtgg gtccacagcc gcgtcaagcg cggcaggtgg
     3181 tattcgagcg cggccagctc gacctgcagg cgtccctccc gggtggtggc gtggcgagcg
     3241 aagatgtcga tgatcagcgc cgagcggtcg agcaccttgc aatcgagcag cttttcgagc
     3301 gcgcgctgct ggttcggcgc cagctcatcg tcgacgatca gcaggttgaa gccggttgcg
     3361 gctcgctcgt cgacgagctc ggctgcgcgg cccttgccga agtaggaggc tgggtcgggg
     3421 tgcgtccggc gctgcgaagc gctcccgacg accaccgctc cggcggtctg ggccagggcg
     3481 gcaagctcgg cgaggctgcg ctcgatcggc cagcgaccgt cgccggggtc gtccaggccg
     3541 atcaggaagg cacgctcctc gggggcagtt aggtcggtga acgaggggtg tgtgggcatc
     3601 tcggaggccc atggtagccg agcctgtccg ggaccagcgc tggcccgctg gtggtcggac
     3661 ggtacttttt ggctacggcg cgaggccaat tgatctgcga tacctctcct gatcccttcc
     3721 ggcacaggag aactcggtga ccgatgcgag cgtgcagccc gacggcagca cgaagagagg
     3781 cgtcccgtcg acggcgagca tggcagtgcg gatgctgatc ccgctcactg ctggcgcagc
     3841 tgcgagcatt gcagcggaga cgctgctcag ccccacgctg cccaacagcc tggtgcgcgc
     3901 cgccaccagc atcgggattg gggcgtgcct ctcggcattc gggttggcga tcatgctgat
     3961 tcgacccctg acgcgctccc gcgccgacct gcaggtccgt taccaggagg cggtcgccga
     4021 cgcccttctt gacccgctga ccggcctggg aaaccaccgc gccttccagg aggagctcga
     4081 cagccagatc gagaacgcca cgcggtacga ggtgcccgtc gccctcgtcc tcatcgacct
     4141 cgacgagttc aagcagatca acgacagcgc cggccatgcc gttggcgacc agaccctggc
     4201 ctctttcggc aggctggtgg ggtccgtgct gcgcaaggtg gaccgtccct tccggatcgg
     4261 cggtgacgaa ttcgccatct tgctgcctca caccgatgcc gacgcggcgc acatcgtggc
     4321 gcgccgcctc ctcgtgtcgg cgctgcagcc caacgttcgg gatccgaaga tcaagcctct
     4381 ctccttctcc gccgggatct cgtcattgcc cggcccggcc accagccgca cgcagctcta
     4441 cacgcaggcc gatacggccc ttcacgcggc caagcacgcc ggacgcaccg aggtgctcgt
     4501 cttcgacccc ggggcggagg tcgccgccac cgccgccagc acctcggccg ccatcgccga
     4561 ggtgatcgag cagaagctgc tgcggcccgt cttccagccg atcgtcaacc tcgcgaccgg
     4621 cggaacgctc ggctacgagg gcctcatccg tcccgtgccg cccgcgccgt acgcggatcc
     4681 ggcgagcctc ttcgccgctg cagaagccag cgggcacgtc gtaccgcttg acctcgcctg
     4741 cgtggaggcc atcgtgtcgg ccgcggcgag cttgccgaag gatctgttcc tgagcgtgaa
     4801 catgtctccg cgcacgatcg aggcgccaga gttcagcgcc cccgccatgc tcaacatcct
     4861 ggcccggtac gatttcccgc ccgagcggct gatcatcgag ctgaccgagc atcagccgat
     4921 cgccgacctc gagcgcgtgc gccataagct cgatacctgc cggagcgccg ggatgcggat
     4981 ggctgctgat gacctgggct cgggcaactc tggcctgcga ctcctgagcg acctgcgctt
     5041 cgacatcgtg aaggtcgacc tcggactcgt gcagcgcagc tcaaccgggg ctcgctcgag
     5101 cgcggtggtc gagtcgatcg tcgccttcgc cagccgaatg ggcgcgctcg tcatcggcga
     5161 gggcgtggag cacgaggagc aggtcgagca gctgacacag ctcggcgtca ccgccgccca
     5221 gggctacctc ttcagtcggc cgggccccct ccccgactgg gcgggcgaga agcgcctgaa
     5281 gcgagcggcc gaccgccaga cgccagccgc ggtcgtggat gaagaccccg acctcgaatc
     5341 gtggcgtcgc aagatcgggt tggcgacccc cgccgcctag gcctcgaccc tggcgcgcgg
     5401 cagcctctcc agcacccgct cggcggcggc gacaagggtc ggatcgtctg cgggcgcctc
     5461 accggccgat aaccacacga tccgctcgtc gcgccggaac caggtgcgct gccgcttggc
     5521 gtactgccgc gtgcgccgga ccgtgaccgc cactgcttct tcgagcgacc actcccccgc
     5581 caggtagtgc gcggcctcgc cgtagccgtg gctggtcagc ggcgcctgtc gcgggtcgtg
     5641 accggcctcc agcaggccgc ggacctcctc gaccaggcca ttggcgaaga gccactgcgc
     5701 tcgctcgtcg atgcgacggt tcaacacctc gatcggacgg ctgatgccaa ccaggctgat
     5761 cggaccttcc catggcttcg ccgccaatgc gctcgcgtcc gtgccagctc tggacgcggc
     5821 gcgttcgagt gcgcgtgcca cgcggcgcgg gttgcgcagg tccgtccgtg ccgcgcccga
     5881 cggatcgacc gtgcgcagcc gatcggccag gacgccgaca ccggcagtcg ccagctcatg
     5941 attcagccgc gcccggtcct ccgcacgcgg cgccggaccg aaggcgtagc catccagcag
     6001 ggcggccagg tacaggccgc tgccagcgac gaccagcggc agccgccggc gggctgtcac
     6061 ctccgtgacc aggcgccgcg ctgcatctgc ccatgaggcg acgctgaacg cttgcccggg
     6121 ggtcacgagg tcgatcaggt gatgtgggac cgcgtccctc gctcgtccat cgggcttggc
     6181 cgtcccgata tccattccgc ggtagacctg gcgcgagtcg gcgaccagga tctcgatcgg
     6241 gattcgcgcc gccagcgcaa gcgacagctc cgtcttgccc gatccggttg ggccgacgat
     6301 gcccgcaatc ggccccaatc agacctcgat gactcgctgc agctgcgtga tcgaggtggc
     6361 gcgaccggtc ccccgctcag cttcgatcag taccgcgttg aatgccaccg gtccctccgc
     6421 gacggcaaac cgggtcggca agtgggtcag gaagcgcggc aggaccgtct ccagcgagaa
     6481 gccgatcacc gagttgcgcg ggccggtcat gcccacgtcg ctgatgtagg cggtgccctt
     6541 cggcaggagc cgggcgtcgg ctgtgggcac gtgggtgtgc gtgcccagca ccgccgtgac
     6601 gcggccgtcg aggtaccagc ccatggcgtt cttctcgctg gtgatctcgc agtggaagtc
     6661 gacgatccgg accaggggca gcggctcggc agccccgtcg agcaggccgt ccatggccgc
     6721 aaacggcgaa tcgagctggt tcatgaagac gcgacccatg acgttcacga ccgccacccg
     6781 atcgccatcg ggcagcgtgt gcagcagcca gcccttgccg ggagcgtcat cgggatagtt
     6841 gagcgggcgc agcaccggcc ggccactgtc gaggtagtcg tagatctcgc gcttgtccca
     6901 gatgtggttg ccgctggtga tgacgtcgac gccattcgcg aacagctcct ctgccaggct
     6961 gggcgtcagc ccggcgcctg ccgccaggtt ctcgccgttg gcgacgacca ggtcgaggtc
     7021 gagctcggtg cgaagatgac gcaggctgcc gacgaccgcc agccggcccg gcttcccgat
     7081 gatgtcgccg atgaccatgc accgcaggag cgcggtgcgg tcggtcatcg agcgtagtcg
     7141 accgcgcgcg tctcgcggat cacggtcacc ttgatctggc ccggatactg gagctgctct
     7201 tcgatcttct tgaccacgtc ccttgccagg cggctggacg ccatgtcgtc catctcctcg
     7261 gggcggacca ggatccggat ctcgcgaccg gcctggatgg cgtagcagcg ctccactccg
     7321 gggaaggcca gtgcgatctg ctgcaggtcg tcgagccgct tgacgtagtt gtcgagcgac
     7381 tcgccgcggg ctcccgggcg ggaggcgctg atggcgtcgg cgatctggac caccgtcgcc
     7441 tcggtcgact cctgctcgac ctcctggtgg tgggcggcga tgcagttgca gaccacggcg
     7501 ttgacgccgt agcgcttggc gatgtcgccg ccgatcaggg cgtgcgggcc gtcgacctcg
     7561 tggtcgatgg ccttgccgat gtcgtgcagc aggccgccct tcttcgactc ggggatgttg
     7621 gcgccgatct cggccgccag caggccgctg aggcgtgcgg tctcaacgca gtggttcagc
     7681 acgttctggc cgtagctggt ccggtacttg agccggccga ggagcttgat cagctcgggg
     7741 tgcaggcctg gcacgccggc gtcgtatgct gcctgctccc ccgcctggcg catcaccacc
     7801 tcgatctcgg cgcgacactt ggccacggtc tcctcgatcc ggccgggatg gatccgtccg
     7861 tcgctgatga gcttggtgag cgccatgcgc gccacctcgc gccggaccgg gtcgaacccg
     7921 gacaggacca ccgcctcggg cgtgtcgtcg atgatcaggt cgaccccggt cgcctgctcg
     7981 agcgcccgga tgttcctgcc ctcgcggccg atgatgcggc ccttcatctc ctcggagggg
     8041 agcggcacga ccgtcaccgt cgcctccgag gtgtggtcgg cggcgatgcg ctgcatgacc
     8101 gtggtcagga cgcggcgagc gtgctcctcg ccctcctcga cggcactgcg ctcgatctcg
     8161 cggaccttct ggtgtgcctc tgcctgcgct tcgtccacga tcagctggat gaggctctgg
     8221 cgcgcctcgg aggcggtgag gccggacact cgctcgagct cgagcagctg ctgctgctga
     8281 agctccacca gccgcgcctt ctcggcgtcg acgtcgcgct gccgctcctc gaacgcgacc
     8341 tcgcgccgtt cgagcgactc cactttgcgg tccaggccct cggcacgatc gagcaggagg
     8401 cgttcctggc gctgcaggtc ggcgcgcttc tctcgcgcct cctcctcggc tgcgcgctgc
     8461 accttgaggg cgtcgtcctt gccctcaagg acgatctcct tctgctttgc gcgcgcctcg
     8521 gccatcagcc gctcggcata gccctcggca tgcttgacgc tgccggcagc caggaagcgg
     8581 cgggacagat atccgattgc aattccaacg atgagggcgg cgccggccgc cacgacgacg
     8641 ttgctttcag gcatcggctc ctccatttgt ggagccccaa tgggcgctcg cgccgcctca
     8701 tgcagcgcgt gtcaattgct gatcacgatc gtcagtccct gccttccatc ggctcggaga
     8761 ctgtagtcgg cgagtgtagc gcaagccgcg gcggcgagcc ttcgggccga tcagcctgcc
     8821 gcgatgcgtc cgagcagggt caccatctcg aggtcgcgcg gcaggaagtc ctgcagcatg
     8881 atgcgctgcg ccccggcggc ctccaggctc tcgatgacct ggcgtgcggc atcaggtgtg
     8941 ccgatgatcc agcgccctgt ccgctcgcgg agccacgtct cggcatctgc gccgcccccg
     9001 ccgagcattg cgatctgctg ctgcactcgc tcgcgcaggt cgctctccgt ctctgccacc
     9061 agcacgccga ccatcgccga gcgcacgacg gtctcggggt cccgtcctgc ctccgcgcag
     9121 gccgtcgcga tgcggccgta ggcctctcgc agccggtcgg gcgttgccga ctgtcggttg
     9181 aactcgtcag cgtaggtggc gaccagccgg gccagccggg gccctccatc cccgcccagg
     9241 accaggtggg gatgtcgctt gccggagaag gtcggtcggg gccgcaggtg ggcgttgcgt
     9301 a
//
LOCUS       DATCQR010000090         3403 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_144563, whole genome shotgun sequence.
ACCESSION   DATCQR010000090 DATCQR010000000
VERSION     DATCQR010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3403
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144563"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..139)
                     /locus_tag="VIN32_03095"
     CDS             complement(<1..139)
                     /locus_tag="VIN32_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY8921598.1"
                     /translation="MATVTFDHVFKRFDGDVVAVNDLNLQVADGEFLVLVGPSGCGKT
                     TA"
     gene            complement(232..1314)
                     /locus_tag="VIN32_03100"
     CDS             complement(232..1314)
                     /locus_tag="VIN32_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015497858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase family protein"
                     /protein_id="HEY8921599.1"
                     /translation="MRLDPTITAVDAHAGGEPGRVIIGGVHDVPGATMLEKRNHLAEH
                     GDQLRHLMLREPRGYPALCANVILPPTDPKADAGFVIMEQAEYPAMSGSNTICTATVL
                     IAEKLVAVTEPITQLTLEAPAGLIRIRAEVRDGRATSITFDNVPAFAIEINAPVDVPQ
                     IGRVKVDVAWGGMFYAIIEASALGLRLTPDEGRDIVRLGEMVKAAAREQIPVAHPDQP
                     EIRGISIVEITAPPTLAGAHGKNTVVVSSGTLDWDRPESFTGVLDRSPCGTGTSARMA
                     VLHARGKLGIGEDFVHEGILGTAWTGRLLRETTVGPYRAVVPQLTGSAWITGRAEYTL
                     DPADPFPEGFTVGDLWGLPDGASSPQ"
     gene            1317..2054
                     /locus_tag="VIN32_03105"
     CDS             1317..2054
                     /locus_tag="VIN32_03105"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY8921600.1"
                     /translation="MHGATPALAGDFAHSDRLVVSLLNARSTAHELLDAEHLDLLELR
                     RNLREMAMLNRLPGGMGASVRAVERLLGEQTEATVLDVGTGSGDFVRRLRRRRHVEVI
                     ASDLRPEVLEIAARNLAGTNHVSLLKADARAIPLADGEVDIAHASLLMHHFDPDDAIA
                     ALAEMRRVSRLGVVINDLRRGLLPFAMTASAVLALTRGAYTRHDGVLSARRAYTLAEL
                     DTLAAKAGLKRSSRSGRFWPRVTTVYR"
     gene            2051..3235
                     /locus_tag="VIN32_03110"
     CDS             2051..3235
                     /locus_tag="VIN32_03110"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY8921601.1"
                     /translation="MSDPTLDCEVLVVGAGPAGSAVAAALAAHGREVLMVEARSHPRP
                     KACAEYASPRIVEELRRLGLTDALWQGDALALTGMRVIRGGDAVDVRYRDAAGPRSAW
                     GLDRLVFDAALAAHATHSGARLMEHATFERPLRTDGLVSGAIVSTPEGSRTVRSRWLI
                     GADGARSRVASGLGVERRVVAPRRLGMVAHYEGDAELSDHGEMHVGPGWYVGLAPLAG
                     GRLNVGMALPLERVRSSTRERFQAAIDGIPAVAARLAGRRRLTQIRGASPIGHRVARA
                     AGPGWLLVGDAAGFIDPFTGEGIYRALRSARAAAESLAAGDDGADERYLAARRHAFAA
                     KDALTWLVQGMLASPPLMGYALRRLASRPATAERLGSALGDCRPASDALSPLFLAQVL
                     RP"
BASE COUNT          448 a         1206 c         1229 g          520 t
ORIGIN      
        1 gcgccgtcgt cttgccgcaa ccggatgggc cgaccaggac caggaactcg ccgtctgcca
       61 cctgcaggtt gaggtcgttg acggcgacca cgtcgccatc aaagcgcttg aacacgtgat
      121 cgaaggtgac cgtcgccatc cgcatcgtcc tcttcggggg tctgcgctcg cccatcgggc
      181 gggtcaacct ggccgcgcgc taccgcgggt cctgcatcat aagacaccgt gtcactgggg
      241 ggaagatgcc ccgtcgggca ggccccacag gtcgccgacc gtgaagcctt cggggaacgg
      301 gtcggccggg tcgagcgtgt actcggctcg tccggtgatc caggcgctcc cggtcagctg
      361 cggcaccacg gcgcggtacg gtccgaccgt cgtctcgcgc agaagccgcc cggtccaggc
      421 cgtgcccagg atcccctcgt gcacgaagtc ctcgccgatg ccgagcttgc cgcgggcatg
      481 gagtacggcc atccgcgcgc tggtgccggt gccacacggg gagcggtcga ggacgccggt
      541 gaatgattcg ggccgatccc agtcgagggt gccggaggac acgacaacgg tgttcttgcc
      601 atgcgcgccg gcgagcgtcg gcggcgccgt gatctcgacg atgctgatgc cgcggatctc
      661 gggctggtcg ggatgtgcca ccgggatctg ctcgcgggcc gcggccttga ccatctcgcc
      721 cagccgcacg atatcgcgcc cctcgtcagg cgtcagccgg aggcccaggg cagaggcctc
      781 gatgatcgcg tagaacatcc cgccccacgc cacgtcgacc ttgacccgcc cgatctgcgg
      841 cacgtcgacc ggagcgttga tctcgatcgc gaaggccggg acattatcga aggtgatgga
      901 ggtggccctg ccgtcgcgca cctcggcgcg gatccggatc aggccggcgg gcgcctccag
      961 ggtgagctgg gtgatcggct ccgtcactgc gaccagcttc tcggcgatga gcacggtggc
     1021 cgtgcagatc gtgttcgatc cgctcatggc cgggtactcc gcctgctcca tgatcacgaa
     1081 gccggcatcg gcctttggat cggtgggcgg aaggatcacg ttcgcgcaga gcgcggggta
     1141 gccgcgaggc tcgcgcagca tcaggtggcg gagctggtcc ccgtgctcgg ccaggtggtt
     1201 gcgcttctcg agcatggtgg cgcccgggac gtcgtggacc ccgccgatga tgacccgccc
     1261 cggctcgccg ccggcgtggg catcgacggc cgtgatggtc gggtccagtc gcatgcttgc
     1321 atggtgccac gcctgcgctg gccggggact tcgcgcatag tgaccgcctc gtggtcagcc
     1381 tcctcaacgc ccgttccacc gcgcatgagc tgctcgacgc ggagcaccta gacctgctcg
     1441 agctgcggag gaacctgcgc gagatggcga tgctcaatcg gcttcccggc gggatgggcg
     1501 ccagtgtgcg cgccgtcgag cggctgctcg gcgagcagac cgaggcgacc gtcctggacg
     1561 tggggacggg ctccggcgac ttcgtccgac ggctgcgccg gcgaaggcac gtcgaggtga
     1621 tcgcttccga cctccggccc gaggtcctcg agatcgctgc tcgtaacctc gccggtacca
     1681 accatgtgtc cctgctcaag gccgatgcgc gcgcgatccc gctcgcagac ggcgaggtcg
     1741 atatcgctca cgcgtcgctg ctcatgcacc acttcgaccc cgatgacgcg atcgccgcgt
     1801 tggcggaaat gcgacgggtc tcgcgactcg gtgtcgtcat caacgacctg cgacgcggcc
     1861 tgctgccgtt cgcgatgact gcctccgccg tcctggccct gacccgcggg gcctacacgc
     1921 ggcacgacgg cgtcctctcc gcgcgccgcg cctacaccct ggccgagctc gacacgctgg
     1981 cggccaaggc cggcctgaag cgcagcagcc ggagcgggcg tttctggccc cgggtcacga
     2041 cggtgtaccg atgagcgacc caaccctcga ctgcgaggtc ctggtggtcg gcgcgggacc
     2101 ggccggctcg gccgttgcgg cggccctggc cgcgcacggg cgcgaggtgc tgatggtcga
     2161 ggctcgctct catcccaggc ccaaggcctg cgccgagtac gccagcccgc ggatcgtgga
     2221 ggagctccgc cggctcggcc tcacggatgc gctctggcag ggggacgcgc tggcgctgac
     2281 cggcatgcgc gtgatccggg gcggggatgc cgtcgatgtt cggtaccggg atgcggccgg
     2341 tcctcgcagc gcgtggggcc tcgaccgtct cgtcttcgac gcggcgctgg cggcccacgc
     2401 gacgcactcg ggcgcccgtc tcatggagca cgcaacgttc gagcggccgc tgcggaccga
     2461 tgggctcgtc agtggggcaa tcgtctccac cccggaaggc tccagaacgg tacggagtcg
     2521 atggctgatc ggggccgatg gcgcgcgctc acgggttgca agcgggcttg gcgtggagcg
     2581 acgggtcgtc gcccctcggc gcctcggcat ggtcgcgcac tacgagggcg acgccgagct
     2641 ctccgatcac ggtgagatgc acgtggggcc cggctggtac gtggggctcg ccccgctggc
     2701 cggcgggcgg ctgaacgtgg gcatggcgct accgctggaa agggtgcgca gcagcacgag
     2761 ggagcggttc caggccgcga tcgacggcat cccggccgtg gctgcacgcc tcgcggggcg
     2821 ccgacgcctg acgcagattc gcggcgcgtc gccgattggg caccgcgtgg cgcgcgccgc
     2881 agggcccggc tggctgctgg tgggtgacgc cgccgggttc atcgacccgt tcaccgggga
     2941 ggggatctac cgtgccctgc gctccgcccg ggctgccgcg gagtccctgg ccgcaggcga
     3001 cgacggcgcc gacgagcgct acctcgccgc ccggcgccac gcgttcgcgg ccaaggacgc
     3061 actgacctgg ctcgtccagg ggatgctcgc ctcccctccg ctcatggggt acgcgctgcg
     3121 gcgactggca tcccggcccg ccacggccga gcggctgggc agtgccctgg gcgactgccg
     3181 tccggccagc gatgcgctct cgcctctctt cctggcgcag gtccttcggc cgtgaagagc
     3241 cgggtccaga tccacatcga ggctccgctg gagcggatct tccccctggc ggccgaggtc
     3301 gagcgctggc cggagcgcct ccctcactac cgctacgtcc atcgcctgcc cgcctcgaac
     3361 ggggagcgac gcttcgcgat gggcgcccgc cgcggcccga tcc
//
LOCUS       DATCQR010000091         5887 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_190595, whole genome shotgun sequence.
ACCESSION   DATCQR010000091 DATCQR010000000
VERSION     DATCQR010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5887
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190595"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..604)
                     /locus_tag="VIN32_03115"
     CDS             complement(<1..604)
                     /locus_tag="VIN32_03115"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEY8921602.1"
                     /translation="MEGGQARGLGLRLGASGLNQRILRLVGRRLPALIGGRMPWLALS
                     RYPIPALPGPDWVRIRPLLAGICGSDTALLTGRASPILSPFASFPAILGHEVVGMVEE
                     AGSAVSVKAGERVVVDPIISCFVRGLDPCSACRDGLPALCRHAADGSLSPGMLIGYCR
                     DLPGAWSESMLAHASQLHRVPDSVSDETAVLVEPLACSLHA"
     gene            complement(613..1773)
                     /locus_tag="VIN32_03120"
     CDS             complement(613..1773)
                     /locus_tag="VIN32_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011172859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEY8921603.1"
                     /translation="MADAVVTRLGSGPAASDRPNGVAVVDGAGGALTAWFLDGVSAEL
                     TTRGYPVQRDRERDATDIDPNVRVVLNAVDADEPRSFRRRSKEIFVVGVAELPDSPDD
                     MLRAGYPLLVRSLSNVFVPIARNGGGPEAYLITMEQGFHRIAGEADDETFFARVVDRL
                     VPLAESRLVIDNEFVPDLPRELWHGDKHTEAIARAGQRLDELDLLPAPWPIDEILSAD
                     ELRHVKRLFGIGGLSYGNASARQSGDDFWMSASGVDKSKLREIGRDILLVTGFDAERG
                     VIRLSVPPEVKPRRVSVDAIEHLTIYREHPEVGAILHVHGWVVGAVSTEAVYPCGTEE
                     LAREVAELVRQAPDPGHAVIGLKNHGLTITGGSLDEILERVGPHIVRQVPMS"
     gene            complement(1766..3106)
                     /locus_tag="VIN32_03125"
     CDS             complement(1766..3106)
                     /locus_tag="VIN32_03125"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEY8921604.1"
                     /translation="MRAVRRVIAIGALLLGAASLAAQAAGADVGVLRLQLTGVIDQVN
                     ASYIEEGIQTAADSGAAAVLIVIDSPGGELTSMDRILKAILGSKVPVITWVAPEGGRA
                     GSAATFITLAGDVAAMAPLTNIGAASVIGGNGEDLPATLGRKVTNDAVARITELARDH
                     GRNETWAESAVRDAVSASVSDAISMKPPIVDIEAADTDALFAEIDKGTRADGHAYEFK
                     GEPLPKLSGLPISDGTMNPGQQFLHLLSDPNIAFILFTIGFYGILSELFHPNLISGSL
                     GAIAIVLAFIGSNSLPLNVGGLLLILMGIGLFVLELHFTSYGFLAAGGVVCFLLGAFA
                     LYTGVDTNNPVEFTVSPFLIVGAILLSLAYFFLLVRGLLQMRANRSTTLPIAALVGTG
                     GTAQTLIAPTGIAYAGGEAWSARSRRGRIQPGKPLRVVAIEGLELIVEPEEKGG"
     gene            complement(3157..4116)
                     /locus_tag="VIN32_03130"
     CDS             complement(3157..4116)
                     /locus_tag="VIN32_03130"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY8921605.1"
                     /translation="MAESNHSLFERRVTVHQLRIFKTVADVRSFTRAAEQLNLSQPGV
                     SHQVRALSDAVGAPLFEQIGRVIHPTETGRLLYDHAILILSEFEAAGRAIDELHGLKR
                     GLLRLVGDTTVGIYVLPDVLGAFKQEHPDVELRLDVGNRQHAYERLLANEADFAVVGR
                     PWPRSDVPLIVRPFLPNELIAIAAPGHPLAGARRVSLARLADEPFIHREPGSGTRETA
                     EEALAKTGRPVHVAMELASNGAIKRAVARGLGVAILSRHAVSLELRLGAVVELPVTGF
                     PLHREWHLVYPRDRRFGPVGEAFLSFVDEGRWRDLVGEGLTTD"
     gene            4226..5233
                     /locus_tag="VIN32_03135"
     CDS             4226..5233
                     /locus_tag="VIN32_03135"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006563088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HEY8921606.1"
                     /translation="MTTARPLLIGIGGDSGTGKSTLTAGVYRIIGERRITTLCLDDYH
                     SLDRRERKLLGVTALDPRANDFFRMESQLLDLKRGQPILKPVYDHHDGTFGTPEEVVP
                     REIVIVQGLHPFLAPGVRALFDLKVWLDPEVELKHRWKIQRDVAKRGYTPEAVAGEIE
                     ARRPDVEAYIEPQRSFADLVIRFHRPPGNEGQEHLNVRITTRSTLPVLNLDGLFEETR
                     AAVRSKSGTDAGRASLILEIDGGIDPGTVRDLEDRIWDHVGSRHQHLRHLAPEQFGDF
                     ADAALQNHHSDPLALTQLLLVHRILSARKSMLLQVDLSAHEASDHDHELEGVVHDHDH
                     P"
     gene            5274..>5887
                     /locus_tag="VIN32_03140"
     CDS             5274..>5887
                     /locus_tag="VIN32_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002731997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016984 - ribulose-bisphosphate
                     carboxylase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RuBisCO large subunit C-terminal-like
                     domain-containing protein"
                     /protein_id="HEY8921607.1"
                     /translation="MTDAQSFEAGVKEYRDAGYWRPDYVPRDTDLLAAFRVTPQPGVP
                     PEEAGAAVAAESSTGTWTTVWTDRLVDLDRYKGKCYRIEPVPGDASQFFAFIAYDMDL
                     FEEGSVANMASSIIGNVFGFKAVRALRLEDLRIPVAYMKTFHGPPHGIQVERDMLNKY
                     GRPILGATIKPKLGMSARNYGRAVYECLRGGLDFTKDDENINSQP"
BASE COUNT         1014 a         2030 c         1945 g          898 t
ORIGIN      
        1 ccgcatgaag ggagcaggca agcggctcga ccaggaccgc ggtctcgtcg ctcaccgagt
       61 ccggaacccg gtgcaactgg ctcgcatgcg ccagcatcga ctccgaccag gcgccgggca
      121 ggtcgcggca gtagccgatc agcatcccgg gtgagagcga tccatcggcc gcgtgacggc
      181 atagagccgg caacccgtcg cggcaggccg aacacgggtc aaggccgcga acgaagcacg
      241 agatgatcgg gtcgaccacc acccgctccc ccgccttcac cgacacggcg ctgccggcct
      301 cctcgaccat gcccaccacc tcgtggccca ggatggccgg gaaggaggcg aatggcgaga
      361 ggatcgggct ggccctgcca gtgaggaggg cggtgtcgct gccgcagatc cccgccagga
      421 ggggtcggat acggacccag tcgggcccgg ggagcgccgg gatcgggtat cgggagagtg
      481 ccaaccaggg catccgcccc ccgatcaacg ccggcaggcg tcggcccacg aggcgcagga
      541 tccgctgatt caggccggat gcgccgaggc gcaggcccag gccgcgagcc tgcccgccct
      601 ccatggcggc agtcagctca tcgggacctg gcggacgatg tgcggcccaa cccgctccag
      661 gatctcgtcc aggctcccgc cggtgatcgt caggccgtgg ttcttcaggc cgatcaccgc
      721 gtgccccgga tccggcgcct ggcgcaccag ctccgcgacc tcgcgcgcca gctcctcggt
      781 gccgcaggga tagaccgcct cggtcgagac ggctccaacg acccagccat ggacgtgcag
      841 gatggccccg acctccgggt gctcgcggta gatggtgagg tgctcgatcg cgtccaccga
      901 gacccgccgc ggcttcacct ccggcgggac gctgaggcgg atgacgccgc gttccgcgtc
      961 gaagccggtc accagcagga tgtcgcgccc gatctcacgc agcttgctct tgtccacccc
     1021 gctggcgctc atccagaagt cgtcgcctga ttggcgagca ctggcgttgc cgtagctgag
     1081 gccgccgatc ccgaacaggc gcttcacgtg ccgcagctca tcggcgctga gaatctcgtc
     1141 gatcggccac ggggcaggca gcaggtcgag ctcgtcgagc cgctggccgg cgcgcgcgat
     1201 cgcctcggtg tgcttgtcgc cgtgccacag ctcgcgcggc aggtcgggca cgaactcgtt
     1261 gtcgatcacc agccgcgact cggcgagcgg cacgaggcgg tcgacaacgc gcgcgaagaa
     1321 tgtctcgtca tcggcctcgc cggcgatgcg gtggaagccc tgctccatcg tgatcaggta
     1381 ggcctccggt cccccgccgt tgcgggcgat gggcacgaac acgttggaga gcgagcgcac
     1441 cagcagcggg tagccggcgc gcagcatgtc gtccggtgag tcgggcagct cggccacgcc
     1501 gaccacgaag atctccttgg agcggcgccg gaagctgcgc ggctcatcgg catccacggc
     1561 gttcagcacc acccgcacgt tcgggtcgat atcggtggcg tcacgctcgc gatcgcgctg
     1621 gacggggtag ccgcgcgtcg tgagctcagc cgagacgccg tcgaggaacc aggcggtcag
     1681 ggcgccaccg gcgccgtcca ccactgccac gccgttcggc cgatcggacg ccgcggggcc
     1741 tgagcccagt cgagtcacga ccgcgtcagc cacccttctc ctccggttcg acgatcagct
     1801 ccagcccctc gatcgcgacc acgcgcagcg gcttgccagg ctggatcctc ccgcgccggc
     1861 tgcgagccga ccacgcctcg ccgccagcat aggcgatgcc ggttggggcg atcagggtct
     1921 gggctgttcc gcccgttccc accagggccg cgatcgggag cgtcgtggat cggttggccc
     1981 gcatctggag caggccccgt accagcagga agaagtaggc gaggctgagc aggatcgccc
     2041 ccacgatcag gaacgggctg accgtgaact cgaccgggtt gttggtgtcg accccggtgt
     2101 acagggcaaa ggcgcccagc aggaagcaga ccacgccacc cgccgccagg aagccgtagc
     2161 tggtgaagtg cagctcgagc acgaacaggc cgatccccat caggatcagc agcagcccgc
     2221 ccacgttcag tggaaggctg ttggagccga tgaaggccag aacgatcgcg atcgccccca
     2281 gcgaaccgct gatcaggttc gggtggaaca gctccgacag gatcccgtag aagccgatcg
     2341 tgaacaggat gaaggcgatg ttcgggtccg acaggaggtg gaggaactgt tggccggggt
     2401 tcatggtgcc gtcgctgatc ggcaggccgg agagcttcgg cagcggctct cccttgaact
     2461 cgtacgcgtg gccgtcggca cgcgttccct tgtcgatctc ggcgaagagg gcatcggtat
     2521 cggccgcctc gatgtccacg atcggtggct tcatcgagat cgcgtcgctc accgacgcgc
     2581 tgactgcgtc ccgcaccgcg ctctcggccc acgtctcgtt gcggccgtgg tcgcgggcca
     2641 gctcggtgat gcgcgccacg gcgtcgttgg tcaccttgcg gccgagggtc gccggcaggt
     2701 cctccccatt gccgccgatc actgacgcag caccgatgtt ggtgagcggc gccatggcgg
     2761 ccacgtctcc cgccagcgtg atgaaggtgg cggcgcttcc ggctcgtccc ccctccggcg
     2821 cgacccaggt gatcaccggc accttgctgc ccaggatcgc cttgaggatc cgatccatgc
     2881 tggtcagctc gccaccgggt gaatcgatca cgatcaggac tgcggctgca cctgagtctg
     2941 ctgcggtctg gatcccctcc tcgatgtacg aggcgttgac ctggtcgatc acaccggtga
     3001 gctggagcct cagcacgccg acatcggctc cggcagcctg agctgcaagg gaggcagcgc
     3061 ctaggaggag ggcgccgatc gcgatgactc gccgcacggc acgcatgcgc cgagtatacg
     3121 cagacgctcc gctccccccg tgcgtgcgcc tatgggtcag tcggttgtca ggccctcgcc
     3181 cacgaggtcc cgccaccggc cctcgtccac gaacgacagg aacgcctcgc cgacggggcc
     3241 gaatcggcga tcccgcgggt acaccaggtg ccactcccgg tggagcggga agccagtaac
     3301 cgggagctcg accacggcac cgagacgcag ctcgagcgag acagcatggc gcgacaggat
     3361 cgcgacgccg aggccccggg ccacggcccg cttgatcgcg ccgttgctcg ccagctccat
     3421 cgccacatgc accgggcgcc cggtcttggc cagcgcctct tcggcggtct ctcgtgttcc
     3481 cgagcccggc tcgcgatgga tgaacggctc gtctgcgagg cgtgccagcg aaacgcgacg
     3541 ggcacctgcc agcggatgcc ccggtgccgc gattgcgatc agctcattgg gcaggaacgg
     3601 ccggacgatc aggggcacgt cggaccgagg ccacgggcgg ccgacgacgg cgaaatcagc
     3661 ttcgttcgcc agcagtcgct cgtaggcatg ctgccgattt ccaacgtcga gccgcaattc
     3721 gacgtcggga tgctcctgct tgaacgcccc gagcacatcg ggaaggacgt agatccccac
     3781 cgtggtgtcg ccgacgagtc gcagcaaccc gcgtttcagg ccgtggagtt cgtcgatcgc
     3841 gcgtcccgcg gcctcgaact ccgaaaggat gaggatcgcg tggtcgtaca acagacgacc
     3901 ggtctccgtc gggtggatca cgcgtccgat ctgctcgaac agcggtgccc cgaccgcgtc
     3961 tgatagcgca cgcacctggt gagagacgcc aggctggctg aggttcagct gctccgctgc
     4021 gcgggtgaag cttcggacgt ctgccaccgt cttgaagatc cggagctggt gcaccgtgac
     4081 gcgccgctcg aagagcgaat ggttggactc agccattcac tcatcttatg cccttgattc
     4141 tcacaatgta ttcgccttca tggaaatgtc gcggtactct cggacatcgg gaacccctac
     4201 ccccggggaa ggcggatgct ctggcatgac gacggcgcgg cccctcttga tcgggatcgg
     4261 cggcgacagc ggcaccggga agtccacgct cacggcgggc gtctacagga tcatcggtga
     4321 acggcggatc accacgctgt gcctggacga ctaccacagc ctcgaccgaa gggagcgcaa
     4381 gctcctgggc gtgacggctc tcgatccgcg ggccaatgac ttcttccgga tggagagcca
     4441 gctgcttgac ctgaagcgag gtcagccaat cctgaagccg gtctacgacc atcacgacgg
     4501 cacgttcggc accccggagg aggttgtccc ccgcgagatc gtgatcgtcc agggcctcca
     4561 cccctttctc gcccccggcg tacgggcgct cttcgacctc aaggtctggc tcgaccccga
     4621 agttgaactc aagcatcgct ggaagatcca gcgcgacgtg gccaagcgcg gctacacgcc
     4681 cgaggcggtc gccggggaga tcgaggctcg gcggcctgac gtggaggcgt acatcgaacc
     4741 tcagcgaagc ttcgctgatc tcgtcattcg cttccaccgc ccgcccggca atgagggcca
     4801 ggagcacctg aacgtccgga tcacgactcg atccacgctc cctgtcctca acctcgacgg
     4861 cctgttcgag gagacgcggg cggccgtgcg gtccaagtcg ggcacagacg ccggcagggc
     4921 ctcgctcatc ctcgagatcg acgggggcat cgacccgggc acggtacgcg acctcgagga
     4981 tcggatctgg gaccacgtcg gttcgcgtca ccagcacctt cgccacctgg cccccgaaca
     5041 attcggagac ttcgccgatg ccgccctcca gaatcaccac agcgatccgc tggcactcac
     5101 gcagctgctg ctcgttcatc ggatcctgag cgcgagaaag tcgatgctcc tccaggtcga
     5161 cctgagcgcc cacgaggcgt cagatcatga ccacgagctg gagggtgtcg tgcatgacca
     5221 cgatcaccca tgaatggctc cccaagaccg cgccccttga aaggaggctg ctgatgaccg
     5281 acgcacaatc cttcgaggca ggcgtcaagg aatatcgaga tgccgggtac tggcgacccg
     5341 attacgtgcc tcgcgacacg gacctgctgg cggcattccg cgtaacaccg cagccgggcg
     5401 tcccgcccga ggaggcgggt gccgccgtcg ccgcggagtc gtccaccggg acgtggacca
     5461 cggtctggac cgatcggctg gtcgatctcg atcgatacaa gggcaagtgc taccggatcg
     5521 agcccgtgcc gggcgatgcg agccagttct tcgcgttcat cgcctacgac atggacctgt
     5581 tcgaggaggg ctcagtcgcc aacatggcgt cctcgatcat cggcaacgtc tttgggttca
     5641 aggccgtccg ggctctccgg ctcgaggacc tgcggattcc agtcgcctac atgaagacat
     5701 tccatgggcc accgcatgga atccaagtcg agcgagacat gctcaacaag tacggacggc
     5761 cgatcctggg ggcgacgatc aagccgaagc tcggcatgtc agccaggaac tacggacgag
     5821 ccgtgtacga gtgccttcgc ggcggactcg acttcaccaa ggacgacgag aacatcaact
     5881 cccagcc
//
LOCUS       DATCQR010000092         4284 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_190652, whole genome shotgun sequence.
ACCESSION   DATCQR010000092 DATCQR010000000
VERSION     DATCQR010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4284
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190652"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..929)
                     /locus_tag="VIN32_03145"
     CDS             complement(<1..929)
                     /locus_tag="VIN32_03145"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEY8921608.1"
                     /translation="MLAVRTYAPGEPLRIEEVPLPKPKGTEIRIRVGGCGVCHTDLHI
                     ARTNRIRVLRPLTLGHEVAGWVDAAGPGAAAELKRARLAEGDGVLVYGGWGCGACAQC
                     VAGEEQRCERSASPGFQRDGGYAEYMLVPRARHLVALGHLHPDHAAPLADAGVTPWRA
                     IQRASAWLQPGARVLLIGFGGLGQFALQYLRFIPDLTVAVRELSPDKLALAGELGADL
                     GLLMGDEALVELGLGGPADVVFDFVGNDQSLDYATRNVAPGGLVSVVGEGGGRYEFSF
                     DRLPVEASLTTTAWGSFADMREVVRLAKRGRLK"
     gene            complement(934..1431)
                     /locus_tag="VIN32_03150"
     CDS             complement(934..1431)
                     /locus_tag="VIN32_03150"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEY8921609.1"
                     /translation="MSTATAGLKVRIGDQIPSVGLRATDGYLLNLRSLVGKQPLILLF
                     FGGPTLKGAARERGDALAEALKEAHPRLTRQGVMVIGVTTDNEHQQAAYVKELELPYL
                     LFSDERRIAVSQLGIPTSESRGNVNAESTAFAVGVDGTILDIVEQAAPKGLVARLLEA
                     IFPPG"
     gene            complement(1428..2327)
                     /locus_tag="VIN32_03155"
     CDS             complement(1428..2327)
                     /locus_tag="VIN32_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEY8921610.1"
                     /translation="MADHLTDGSALARVRSSLLAWYATDHRDFPWRRTADPYAVLVSE
                     VMLQQTQASRVAERFPTFLSRFPTAAALGAAREAEVLAAWSGLGYNRRALALRRAATE
                     VANRGWPQDVAGLERLPGIGPYTARAIASLAFGEAIGVVDTNVRRWLVRRFGLRADGP
                     TRELQGLADALATAGNAADAAPWTHATMEFGASICTPRNPHCDACPISDGCPSRGVAT
                     PVPVPRQAPFAGSDRAHRGAILRALTQAHDHRMTTRLARDLVPPGVVGRVVAGLERDG
                     LLHRSAGRLVLGGPSDPAATIRP"
     gene            2401..3123
                     /locus_tag="VIN32_03160"
     CDS             2401..3123
                     /locus_tag="VIN32_03160"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEY8921611.1"
                     /translation="MRYRLLCLDAGFTLLAPKSTLGEALRGVLGERGHDVTDEQVHQA
                     WEVADRWFWDNYQRPENDTWGDDTKIDAAWREYHSLMLRELGIDDPGHQLIDTILAAQ
                     YAPESWELYPDVLPAVRELRELGLRLGVVSDWGSNLLPIVDGLGLGGELDFVIASGAV
                     GLSKPDPAIFRLAATRAGVPAGEALMVGDSYRADIEGAESAGMDGILIRRPEWEERRE
                     PIPPEVRVIASLAELPEVVRSS"
     gene            complement(3120..4082)
                     /locus_tag="VIN32_03165"
     CDS             complement(3120..4082)
                     /locus_tag="VIN32_03165"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY8921612.1"
                     /translation="MISRRPSSNLLGALLIVIAASSFGVLGPIAQYADRAGVSSLTLV
                     TWRAGVGAIVVLVFLAVRAAGGTRLWRPWRELPGRDRLFMAMAAPTNAALNLAMFVAF
                     LRIGIALALLIFYVYPAFVALASMLWFGERLDRIRWAALGISMAGVVLVVAGAGDLGS
                     LDALGIGLAFIGALAQTFYALAAHHGFRAVPGTQAAAFTMGGAAILYLLLFALIGRLP
                     EVGQPLAGIAALWPVMVAGVVGAGLATVCFIGGIRLLGAPRATILSTLEPVVGVGLAA
                     ILFGTLPTALQVAGGALIIAAGIVLQLRPSGEIAEHEAIAESPG"
     gene            complement(4079..>4284)
                     /locus_tag="VIN32_03170"
     CDS             complement(4079..>4284)
                     /locus_tag="VIN32_03170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921613.1"
                     /translation="ETDASLAEMSERLADPATYADHHAFRELIDRHNAARDRADQLGA
                     DWARLSSELEAAEAEDSLIPTAR"
BASE COUNT          684 a         1497 c         1539 g          564 t
ORIGIN      
        1 cacttcagcc gaccacgctt ggcgagccgg accacctcgc gcatgtcggc gaacgagccc
       61 caggcggtgg tcgtcaggct ggcctcgacc ggcagccggt cgaagctgaa ctcatatcgg
      121 cccccgccct cgccaaccac cgagacgagg ccgcccggcg ccacgttgcg ggtcgcatag
      181 tcgaggctct gatcgttgcc aacgaagtcg aataccacgt cggcggggcc accaagcccc
      241 agctcgacca gcgcctcgtc gcccatcagc agtcccaggt ccgcgcccag ctcgccggcc
      301 aatgcgagct tgtcggggct cagctcgcgg accgccacgg tcaggtcggg gatgaagcgc
      361 aggtactgga gcgcgaactg gccgagcccc ccaaagccga tgagcaggac gcgggctccc
      421 ggctgcagcc acgccgaagc gcgctggatg gcacgccacg gcgtgacccc ggcatcggcc
      481 agcggagcgg cgtgatcagg gtggaggtgc ccaagggcca ccaggtggcg ggcgcgtggc
      541 accagcatgt attcggcgta gccgccgtcc ctctggaatc cgggcgacgc ggagcgctcg
      601 cagcgctgct cctccccggc cacgcactgt gcgcaggcgc cgcagcccca gccgccgtag
      661 accagcactc cgtctccctc ggccaaccgc gcccgcttga gctcggcggc ggcgcctgga
      721 ccggcagcat cgacccagcc ggcgacctca tgccccaggg tgagcggccg aaggacgcgg
      781 atccgattgg tccgtgcgat gtgcagatcg gtatggcaca ccccgcaacc gccgactcgg
      841 atgcgaatct ccgtgccctt cggcttcggg agggggacct cttcgatgcg cagcggctcg
      901 ccgggggcgt aggtccggac ggcgagcatc ggcctagccc ggtgggaaga tcgcctcgag
      961 cagacgggcg acgaggccct tcggagcggc ctgctcgacg atgtccagga tcgttccgtc
     1021 gactccgacc gcaaatgccg tcgactcggc gttcacgttg ccgcgtgact cactggtggg
     1081 aatgcccagc tgcgagactg cgatccgtcg ctcgtcggag aagaggaggt agggcagctc
     1141 cagctccttc acgtaggcgg cctgctgatg ctcgttgtcg gtggtgacgc cgatgaccat
     1201 cacgccctgc ctggtgagcc gtggatgtgc ctccttgaga gcctcggcca gcgcgtcgcc
     1261 gcgctcgcga gcggctccct tgagcgtggg gccgccgaag aagaggagga tcagaggctg
     1321 cttcccgacc aggctccgca ggttcagcag gtagccgtcg gtggctcgca gcccaacgga
     1381 cgggatctgg tcgccaatcc tcaccttcag gccggccgtc gcagtgctca tgggcggatt
     1441 gtagcggcgg gatccgaggg accgccaagg accaggcgtc cggcggagcg atgcaggagg
     1501 ccatcccgtt ccaggccggc gacgacgcgc cctacgacgc caggcggtac gaggtcgcgc
     1561 gccaaccgcg tcgtcatcct gtgatcgtgg gcttgggtaa gggctcgcag gatcgcgccg
     1621 cgatgggctc gatcagaacc cgcgaacggc gcctgccgag gcaccgggac aggggtggcc
     1681 acgccacgcg acgggcatcc gtcggatatg gggcatgcat cgcagtgggg gttgcgcggg
     1741 gtgcagatcg aggccccgaa ctccatggtg gcgtgcgtcc agggcgccgc gtcagcggcg
     1801 ttgccggctg tggcgagggc atcggcgagg ccctggagct cgcgagtcgg gccgtctgcc
     1861 cgaaggccaa accgacggac cagccagcga cggacgttcg tgtcgaccac cccaatcgcc
     1921 tccccgaagg cgaggctggc gatggctcga gccgtgtacg gcccgatccc cggcagccgc
     1981 tcgagcccgg ccacgtcctg cggccagcca cgatttgcga cctcggtcgc cgcacggcgg
     2041 agggccaggg cgcgtcggtt gtagccgagg ccggaccacg cggccaggac ctccgcctcg
     2101 cgtgcggcgc ccagggctgc ggccgtcgga aatcggctca agaaggttgg gaaccgctcg
     2161 gcaacgcgcg aggcctgggt ctgctggagc atcacctcgc tgaccaggac ggcgtacgga
     2221 tcggcggtgc gacgccatgg aaagtcacgg tgatcggtcg cgtaccacgc cagcagggag
     2281 gagcgaacgc gcgccagcgc ggagccgtcg gtcaggtggt cggccatccc gggagggtac
     2341 gccgcaactg atcagatcac ttgaacactt gggcgccggg tcacgctacc gtgtgtcggg
     2401 atgcgctacc gccttctctg cctggatgcc ggctttacgc tcctcgcgcc caagagcacc
     2461 ctcggcgagg ccctccgcgg cgtgctcggc gagcgcggcc acgatgtcac cgacgaacag
     2521 gtgcaccagg cctgggaggt cgccgaccgc tggttctggg acaactacca gcgtcccgag
     2581 aacgacacgt ggggcgacga cacgaagatc gacgcggcct ggcgcgagta ccactcgctc
     2641 atgcttcgcg agctgggaat cgacgatccc ggccaccagc tgatcgacac gatcctggcg
     2701 gcgcagtacg cccccgagtc gtgggagctc taccccgacg tgctgccggc ggtccgcgag
     2761 ctgcgggagc tgggcctgcg gctcggcgtc gtctcggact ggggctcgaa cctgctgccg
     2821 attgtcgacg ggctcggcct gggaggggag ctggacttcg tgatcgcctc gggcgccgtg
     2881 ggcctctcca agcccgaccc cgccatcttc cggctcgccg ccacgcgcgc cggcgtcccc
     2941 gcgggagagg cgctgatggt gggagactcg taccgggccg atatcgaagg tgccgaatcg
     3001 gccgggatgg acggaatcct catccgccgg ccggaatggg aggagaggcg ggagcccatc
     3061 ccgcccgagg tcagggtgat cgcctcgctg gcagagctgc cggaggtcgt ccgctcgagc
     3121 tagccgggag actcggcgat cgcctcgtgc tcggcgatct caccgctcgg acgcagctgc
     3181 agcacgatcc cggcggcgat gatcagcgcc ccgccggcaa cctgcagggc ggtcggcagc
     3241 gtcccgaaca ggatcgccgc caggccgacg ccgacgaccg gctcgagggt cgagaggatg
     3301 gtcgcgcgcg gcgcgccgag cagccgaata ccaccgatga agcagacggt ggccagtccg
     3361 gcgcccacca cccccgcgac catgaccggc cacagcgccg cgatgccggc cagcggctgg
     3421 cccacctcgg gcaggcggcc gatcagcgcg aacagcaaca ggtagaggat ggcggcgccc
     3481 cccatggtga aggccgcggc ctgggttccc ggcaccgcgc ggaagccgtg gtgcgcggcc
     3541 agggcataga acgtctgggc cagcgcgccg atgaacgcca ggccgatccc cagcgcgtcg
     3601 agggatccga ggtcgccggc gccggccacg accagcacca cgccggccat cgagatgccg
     3661 agcgccgccc accggatccg gtcgagccgc tccccgaacc agagcatgct ggccagcgcc
     3721 acgaaggcgg ggtagacgta gaagatcagc agcgccagcg cgatgccgat gcgcaggaag
     3781 gcgacgaaca tcgccaggtt gagggccgcg ttggtggggg cggccatcgc catgaagagc
     3841 cgatcgcgcc ccgggagctc acgccatggt cgccacagcc gcgtcccgcc ggcggcccgg
     3901 acggcgagga agacgagcac cacgatggcg cccacccccg cgcgccaggt caccagagtc
     3961 aggctcgaga ccccggcacg gtccgcgtac tgggcgatcg gtcccagcac cccgaacgac
     4021 gacgccgcga tgacgatcag cagcgcgccg aggaggttcg acgaggggcg ccgggagatc
     4081 atcgggcggt tgggatgagg ctgtcctctg cctccgctgc ctccagctcg gagctcagtc
     4141 gcgcccaatc ggcgcctagc tggtcggctc ggtcacgggc cgcgttgtga cgatcgatca
     4201 gctcgcggaa tgcgtggtga tcggcgtagg tggcagggtc tgccaggcgc tcgctcatct
     4261 cggccaggga ggcatcggtc tctc
//
LOCUS       DATCQR010000093         3899 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_190675, whole genome shotgun sequence.
ACCESSION   DATCQR010000093 DATCQR010000000
VERSION     DATCQR010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3899
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190675"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            196..2010
                     /locus_tag="VIN32_03175"
     CDS             196..2010
                     /locus_tag="VIN32_03175"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HEY8921614.1"
                     /translation="MNRFAKLAVAASIATYVLIVVGGLVRATDSGLGCPDWPACFGKW
                     VPPAELHAWIEHSHRLVAALAVGPLVAAVGLITLFTARRRDRPLLAAALITGILVILQ
                     ALLGGQVVIQQLRAELVTAHLAMALTVLALTILIADRATSGALPPSAAGLPGRLVAVT
                     GIAVFAQMLLGSWVSGTAAGLAFPDFPLMDGGVWPSTVADGAVVQLAHRALAVVVALL
                     VLWTAGRVRRAVVAPRTRRLAGLVVVLVLVQIALGAANVWSRLSALFVVPHLAVGAAL
                     WATIVLLYLAVRRSPAPEPTRQMLVEGRAIAAPVLTLGETLRAYLALTKPRIIELLLV
                     TTVPTMVLAQRGIPSIWLMAAVVLGGSLAAGGANTINMYIDRDIDDLMRRTRHRPLPR
                     HAVTPQRALIFGIVLSILSFVWLTLTVNLLSALLAASAIGFYVFVYTMWLKRSTPQNI
                     VIGGAAGCVPVLVAWAAVTGTVQVPALVLFAIVFYWTPPHFWALALRYRGDYEAASVP
                     MLPVVRGEAETARQIVVYTILLVVVSLLLFPAARMGLIYLAAAVVLGVGFVWYALRVQ
                     RDTTDGRAAIRLFRFSISYLTLLFAAVAADSLLRLAPG"
     gene            complement(2014..2970)
                     /locus_tag="VIN32_03180"
     CDS             complement(2014..2970)
                     /locus_tag="VIN32_03180"
                     /inference="COORDINATES: protein motif:HMM:NF021214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HEY8921615.1"
                     /translation="MRTRLAIPLLAAVTVALPGRTLAHGGATPAPSFPGVLLQWRLEP
                     LVVVALATAAIAWLILARRVALRHPGHPHPAWRSAAFLGGLAVLALALLSPIEAYEGQ
                     LFSVHMLQHMLLELVAAPLLLLGAPATLALRAASPPVRARLLTVLHSRIVTVLSFPLL
                     AWVLFAAVNWGWHFSTLYDQALETPWLHDLQHLTFLGAALLFWWPVVGADPARWRLPH
                     PVRLFYLFLAMPQNSFLGIALMSAPAALYSHYLTNVRDWGPTPAVDQSVGGMLMWVGG
                     DVVFLLAMGLVVAAWVRAEDRRTARADAREDARIAQTQPPAG"
     gene            3049..>3899
                     /locus_tag="VIN32_03185"
     CDS             3049..>3899
                     /locus_tag="VIN32_03185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921616.1"
                     /translation="MRRIALPLLPAAVALSILLAACRGVVGGASASTGPIAHPSGDAL
                     ILGIDYSGGFVAPGFRLTAFPTFSLTGDGRVIVPGPQIELFPGPALPAVNVRRLNEAG
                     IQAVLGLVTRTARFDASAEYPGAHNFIADAADTVFTLHADGKDVQVVVYALGTLDPAG
                     NYPGMSADELAAHRALSGLLERLGAMDDWMPAYDWAEATWQMYQPDALRLVVRNADAD
                     PPDESGIGNMLLDWPVASDPAVFGNPAFDDLRCGVVSGEEAKDWYAALGSANQLTRFV
                     KGDHRYQV"
BASE COUNT          525 a         1347 c         1377 g          650 t
ORIGIN      
        1 tcgcatctgc cagcaggcgg ttgctgcctc tggtgccgga cctgctgcgc cacgtcccgg
       61 cggcccgttc cgcggaggcc aggctgctac gggccagggc tgcacggtga cgcggttggt
      121 tcgggtcggt atgatgccgg cagcccattc cggcactgag atgcaccgcc gtcgcggcgt
      181 ggttcgagga acctgttgaa ccgattcgcg aagctcgccg tggccgcgtc gattgcaacc
      241 tatgtgctga tcgtggtcgg cgggctggta cgcgccaccg attcaggatt gggctgcccg
      301 gattggccgg cctgcttcgg gaagtgggtc ccacctgcgg agctgcacgc gtggatcgag
      361 cacagccatc gtctggtggc ggcgctggcg gtcggaccgc tcgtcgcggc agtggggctg
      421 atcaccctgt tcactgcgcg gcgccgcgat cgcccgctgc tcgccgcggc gctgatcact
      481 ggcatcctcg tcattctgca ggcgctgctc ggcggccagg tcgtgatcca gcagctgcgc
      541 gccgagctgg tgaccgccca tctcgcgatg gcactcacag tgctggcgct gacgatcctc
      601 atcgcggatc gcgccaccag cggtgcgctg ccgccgtcgg cggccgggct gcctggaagg
      661 ctggtcgcgg tcacggggat cgccgtgttc gcccagatgc tgctcggctc gtgggtctcg
      721 gggacggcgg cgggcctcgc cttcccggac ttcccgctga tggatggcgg cgtgtggccg
      781 tcaacggtgg ctgatggcgc cgtggttcag ctcgcgcacc gcgcgcttgc ggtggtggtg
      841 gcgctcctcg tcctgtggac cgcgggccgc gtccgccgag cggtcgttgc gccacgcact
      901 cggcggcttg ccggcctggt cgtcgtcctc gtgctcgtcc agatcgcgct tggggcggcc
      961 aacgtatggt cacgcctgtc agccttgttc gtggtgccgc acctcgccgt cggggctgcc
     1021 ctgtgggcca cgatcgtgct gctctacctc gcggtgcggc gatccccggc gccggagccc
     1081 acgaggcaga tgctggtcga gggccgcgcg atcgccgcgc ccgttttgac cctcggcgag
     1141 accctgcggg cctacctggc gctcaccaag ccgcgcatca tcgagctgct gctggtcacc
     1201 accgtgccga ccatggtcct cgcgcagcga ggcatcccgt cgatctggct catggctgcg
     1261 gtggtgctgg gaggctcgct ggcggcgggg ggcgccaaca ccatcaacat gtacatcgac
     1321 cgcgatatcg acgacctgat gcgccgtacc cgtcaccgtc cgctacctcg ccacgccgtg
     1381 acgccacagc gggcactcat cttcgggatc gtgctgagca tcctctcctt cgtgtggctg
     1441 accctgaccg tcaatctcct gtcggcgctc ctcgcggcga gcgccatcgg cttctacgtc
     1501 ttcgtctaca cgatgtggct caagcgatcg acgccgcaga acatcgtcat cggcggcgcc
     1561 gccggctgcg tcccggtcct cgtcgcctgg gcggcggtga ccggcacggt ccaggtgccg
     1621 gcgctggtgc tgttcgccat cgtcttctac tggaccccgc cgcacttctg ggccctcgcc
     1681 ctccggtatc gcggggacta cgaggcggcc agcgtgccga tgctgccggt ggtccgtggt
     1741 gaagccgaga ccgctcgcca gatcgtcgtc tacacgatcc tgctggtggt cgtctcgctc
     1801 ctcctcttcc cggcggcgcg gatgggcctc atctacctgg ctgctgcggt ggtcctgggc
     1861 gtgggcttcg tctggtatgc cttgcgggtc cagcgggaca cgaccgatgg tcgggcggcg
     1921 atccgcctgt tccgtttctc gatcagctat ctgaccctgc tcttcgcggc ggttgccgcc
     1981 gattcgctgc tgaggcttgc gccgggctga cggtcagcct gccggcggct gcgtctgcgc
     2041 gattcgggcg tcctcgcggg cgtcggcacg cgcggtgcgg cggtcctcgg cccgtaccca
     2101 ggctgcgacc acgagcccca tggccagcag gaagacgacg tcgccgccga cccacatcag
     2161 cattcctccc acgctctgat cgacggcggg ggtcggcccc caatcccgca cgttggtcag
     2221 gtaatgcgag tagagggcgg caggtgcgct catcagggcg atccccagga acgagttctg
     2281 gggcatggcc aggaacaggt agaagagccg aaccgggtgt ggcagccgcc atcgggcggg
     2341 gtcggcgccc accaccggcc accagaagag gagcgcagcg cccaggaagg tgaggtgctg
     2401 gaggtcatgc agccacggcg tctcgagggc ctggtcgtag agggtgctga agtgccagcc
     2461 ccagttgacc gcagcgaaca gaacccaggc cagcaacggg aaggagagca cggtcacgat
     2521 ccggctgtgc agcacggtaa gcagccgagc tcgcaccggt ggtgacgcgg cgcgcagtgc
     2581 cagcgtcgct ggcgctccga gcagcagcaa tggagcggcg accagctcca gcagcatgtg
     2641 ctggagcatg tggacgctga agagctggcc ctcgtatgcc tcgatcgggg agaggagagc
     2701 cagggcgagg acggccaggc ccccgaggaa ggcggcgctt cgccaggcag gatgcgggtg
     2761 gccggggtgc ctcaacgcca cacggcgcgc caggatcagc caggcgatcg ccgcggtcgc
     2821 cagcgcaacg accacgagcg gctcgaggcg ccactggagc agcaccccgg gaaacgaagg
     2881 agccggggtc gcgccgccat gggccagcgt ccggccgggc agcgcgacgg tgacggccgc
     2941 caagagcggg atcgccagcc tggtgcgcat gtccgctgca ttctcgccct agccggatcg
     3001 taccgctggg gacgggaacc ctgcggggga tcgacgcgtc caacgggaat gagacgaatc
     3061 gccctcccgc ttctgcctgc cgccgtcgcc ctgtccatcc tgctggcggc ctgcaggggc
     3121 gtggtgggcg gggcgagcgc gtcgaccggc cccatcgccc atccgtcggg agacgccctg
     3181 atcctgggga tcgactactc cggcggattc gtcgcgccag gcttccgctt gaccgccttt
     3241 cccacgttca gcttgaccgg ggacggccgg gtgatcgtcc ccggcccaca gatcgagctg
     3301 ttcccgggcc ccgcgctccc ggccgtcaac gtccggcgcc tcaacgaggc aggcatccag
     3361 gctgtgctcg gtctcgtgac gcgcaccgcg cggttcgacg ccagcgccga gtacccaggt
     3421 gcgcacaact tcatcgctga tgcggccgac acggtcttca cgctccacgc ggacgggaag
     3481 gatgtccagg tcgtggtcta cgcgctcggc acccttgatc cggccggcaa ctatccgggc
     3541 atgtcggccg acgagctggc cgcgcaccgc gccctgtcag ggctgctcga gcgtctgggc
     3601 gccatggacg attggatgcc ggcctatgac tgggcggagg cgacctggca gatgtatcag
     3661 cccgatgcgc ttcggcttgt ggtccgcaac gccgacgcag atccgccgga cgagagcggc
     3721 atcggcaaca tgctgctgga ctggccggtc gcctccgacc cagccgtgtt tggcaaccct
     3781 gccttcgatg acctgcgctg cggcgtcgtc agcggcgagg aggcgaagga ctggtacgcg
     3841 gcccttggct ccgcgaacca gctgacgcgc ttcgtcaagg gcgaccatcg gtaccaggt
//
LOCUS       DATCQR010000094         1954 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_221655, whole genome shotgun sequence.
ACCESSION   DATCQR010000094 DATCQR010000000
VERSION     DATCQR010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1954
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221655"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..914)
                     /locus_tag="VIN32_03190"
     CDS             complement(<1..914)
                     /locus_tag="VIN32_03190"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEY8921617.1"
                     /translation="MRESAGTPPRHRPEAGGIVVSWPGADGQPAGRWPHRHLLDVDQL
                     TLGELEQCLDLAVEMRERRKRREALDLLRDEVVGLAFCEPSTRTRVSFELAARALGAE
                     VVDLPIEASSLTKGESLVDTLRTLEQTGVTTIILRHPMSGAPYLAARSTGARIVNAGD
                     GSHAHPTQALLDALTLREALGPLDGKKIAIVGDIGHSRVARSNLHSLTALGASVWVAG
                     PPAWVTGFEGWPGVTVASRLEEALADADAVMALRIQLEREAGGISLRDYTAEWGLDEA
                     HMSLARPGAPLLHPGPTNEGVEITADLAA"
     gene            complement(989..1546)
                     /gene="pyrR"
                     /locus_tag="VIN32_03195"
     CDS             complement(989..1546)
                     /gene="pyrR"
                     /locus_tag="VIN32_03195"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEY8921618.1"
                     /translation="MSERGILDADELRRALTRIAHEIVERNGGTDALVLVGVRSRGVP
                     LARRLAGLIEQHEGVSLPVGALDITFYRDDLTKIAHAPIVKKTQAMPEIDDRTVVLVD
                     DVLFTGRTVRAALDALTDHGRPLAVRLAVVIDRGHRELPIRADHVGKNLPTSREEIVH
                     VRVVEEDGADEVVIERSQAAEASTG"
     gene            complement(1595..>1954)
                     /locus_tag="VIN32_03200"
     CDS             complement(1595..>1954)
                     /locus_tag="VIN32_03200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921619.1"
                     /translation="WGPDSDHFAFWDGAWTGAPQSADGTYPDQADAYVGSFSGGLLSS
                     ASALALVRPADAYLVAIAFAQDGTSVAITLGLPSAGIGDPPSALLQIVPLDGSAPHGV
                     GGGADAPPWNGPAVYGY"
BASE COUNT          300 a          688 c          660 g          306 t
ORIGIN      
        1 gctgccaggt ccgcggtgat ctcgactccc tcgttggtcg ggccgggatg gaggagcggc
       61 gcacctggac gcgccaggct catgtgggcc tcatcgagac cccattcggc ggtgtagtcg
      121 cgaagggaga tgcctcccgc ctcgcgctcg agctggatgc ggagcgccat caccgcgtcg
      181 gcatcggcga gggcctcctc cagccggctt gcgacggtga cccccggcca gccctcgaat
      241 cctgtgaccc acgcgggcgg cccggccacc cataccgatg caccgagcgc ggtcagcgaa
      301 tgcaggttcg agcgtgccac ccgcgaatgc ccgatgtctc ccacgattgc gatcttcttc
      361 ccgtccagcg gacccagtgc ctcgcgcagc gtcagcgcgt ccagcagcgc ctgggtggga
      421 tgggcatgcg aaccgtcgcc ggcgttcacg atcctggcgc ccgtgctgcg cgcggcgaga
      481 tagggggcgc cactcatggg gtggcgcagg atgatggtcg tgacgccggt ctgctcgagc
      541 gtgcgcagcg tgtcgaccag cgactcgccc ttggtgaggc tgctcgcctc gatcgggaga
      601 tcgaccacct cggcgccgag cgcccttgcg gccagctcga agctgacgcg agtgcgggtc
      661 gacggctcgc agaaggccaa tcccacgacc tcgtcgcgga ggaggtccag cgcctcgcgc
      721 cgcttgcgtc tttcgcgcat ctcgaccgcg aggtcgaggc attgctcaag ctcgcccagg
      781 gtcagctgat cgacatcgag caggtggcgg tgcggccacc tgccggcggg ctgcccatcg
      841 gcgccgggcc atgacacaac aatgccaccg gcttcaggcc gatggcgtgg cggcgttcct
      901 gcggattcgc gcaatcaggg tctccttggc ggcctcacag gaccgtcctt aaagggctcg
      961 gcacagtata gtcacgagcc gccgcggctc agccggtcga cgcctcggca gcctgcgatc
     1021 gttcgatgac cacctcatcg gccccgtcct cctcgacgac gcggacgtgc acgatctcct
     1081 cgcggctggt cggcaggttc ttgccaacgt gatcagcacg gatcgggagc tcgcggtgcc
     1141 cgcggtcaat caccaccgcc agccggaccg ccagcggccg tccgtgatcg gtcagggcgt
     1201 cgagcgccgc gcgaaccgtt cgccccgtga acagcacgtc gtcgaccagc acgaccgtcc
     1261 gatcgtcgat ctccggcatg gcctgcgtct tcttgacgat cggggcgtga gcgatcttcg
     1321 tcaggtcatc gcggtagaag gtgatgtcca gcgctccgac cggcagactg accccttcgt
     1381 gctgctcgat cagcccggcg agcctgcgcg ccaggggcac tccccggctg cgcacaccca
     1441 ccagcacgag ggcatcggtc ccgccgttgc gctcgacgat ctcgtgcgca atccgggtga
     1501 gtgcgcgacg cagctcatcg gcgtcgagga tccctcgttc gctcatgcag ccgagtgtag
     1561 cccgatgggt tggccgcgca cccgacctgc gaggtcagta gccgtagacg gccgggccgt
     1621 tccagggcgg cgcatcagcc cctccgccga caccatgggg agcgctcccg tccaggggca
     1681 cgatctgcag gagagcggat gggggatccc cgatgcccgc ggacgggagg cccagggtta
     1741 tcgccacgga ggttccgtcc tgggcgaacg caatggcgac aaggtacgca tcagcgggtc
     1801 ggacgagcgc cagcgcagag gcgctgctca aaaggccacc gctgaagctg ccgacgtagg
     1861 catcagcctg gtccggatag gttccgtcag cactctgcgg tgctccggtc catgccccat
     1921 cccagaacgc gaaatgatcg ctgtcgggac ccca
//
LOCUS       DATCQR010000095         6253 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_97790, whole genome shotgun sequence.
ACCESSION   DATCQR010000095 DATCQR010000000
VERSION     DATCQR010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6253
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97790"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..821)
                     /locus_tag="VIN32_03205"
     CDS             complement(<1..821)
                     /locus_tag="VIN32_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase domain-containing protein"
                     /protein_id="HEY8921620.1"
                     /translation="MVPADASVQLEIDGFEVAISNPGKLFFEQAGVTKLDLVNYYLAV
                     APGALRGVMDRPMALKRFVHGAAGEAFFQKRAPATVPEFVRTVELSFPSGRTADEVVV
                     SNGAALAWVVNLGCIDLNPHPLRADDLDHPDELRVDLDPGPGVPWSAVRDVAVVAGEV
                     LADHGLTGWPKTSGSRGIHVYARIERRWTFSEVRRSALALAREVERRVPDLATSKWWK
                     EERHGVFIDYNQNAKDRTVASAYSVRPVPDARVSAPLLWSEVPTAELADFTVKTMP"
     gene            842..1726
                     /locus_tag="VIN32_03210"
     CDS             842..1726
                     /locus_tag="VIN32_03210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEY8921621.1"
                     /translation="MVERWKRLQEAANGWASRWRTTRVGRRALAGFALHDGPSVASSM
                     AYFAILSLFQVIVLGVIVFSLLIGEGEARRIVIGRLESALPLQPGTVTSVVESIIAAR
                     GGVTIVSVPLLAWGAIGFFGAMSTGVGRAFATSTRRPFWQDKLIALLLLGGSGLLTLI
                     AVSIGLGESIAARLADRIPGGGRGGQLLVDLVGFALPILLVLVALLVIYRVVPNRRVT
                     VRQVLPGAIVATILFTVLRSGFTWYATDVARYESFFGPISTVISLLVFLHFASMVVLL
                     GAEIARANVLEDDEGLDK"
     gene            1855..3180
                     /locus_tag="VIN32_03215"
     CDS             1855..3180
                     /locus_tag="VIN32_03215"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEY8921622.1"
                     /translation="MRRRSHLAIMVIALLMVGTSIVTTSPRDQAQAADPLAEAKAQRQ
                     ALEATLSAQRAALAQLRSKASTLAGKLDAARAQLASVTAEYDHVSGLLAEVQAEVADI
                     TARLADLRAQVASLDKQLQGVAIEIIAQTDELHTRESLLQDHMRSAYERSQTSVLELL
                     LSADSLDAATNQVSYMITVSDQDKQLAIEIRDLRASLETKEESLRAGRLEVAAARDQA
                     NAEAKALAQREAELATMQAELARLQSAADQKRAEQEAALNAALQAKGNVAQQIADSQK
                     AFDAQTKLVAKLQAEEDARRQAPSAFGFKWPELTFQVTQEWGPTGFVLEPSYTYNGTY
                     YSHFHGGIDIGNGCGTPILAVGDGTVRASGQPLWPWDTGYGVVISHGSGVQSWYWHLR
                     AQVIVSPGQVVSRGQVIGYEGNTGNSTGCHLHFAINDHGIWENPRVYLP"
     gene            complement(3177..5360)
                     /gene="glgP"
                     /locus_tag="VIN32_03220"
     CDS             complement(3177..5360)
                     /gene="glgP"
                     /locus_tag="VIN32_03220"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HEY8921623.1"
                     /translation="MTSAVRVPSQPGYDFGLPPALEGLGELAYNLWWSWTPRAGALFA
                     RIDSAAWSRYRSPIPVLAAVEPGRWTELLADEDFMVDASRVLDEFERYLANGSDSWYR
                     AATDSDPSATLPGPIAYFCAEYGIHESMQIYSGGLGILAGDHCKSASDAALPFVGVGL
                     LYRRGYFRQQIDADGHQEHAQPDLDPAMLPLRRARGRDGSPLQVSVDFADRKVHAAVW
                     LAQVGRVPLLLLDTDIPANEGSDRPITHILYVRGREMRLCQEIVLGIGGVRALRGLGI
                     EPAAWHLNEGHSAFLLLERARELVAAEPGLGADEALRRVGQNSVFTIHTPVKDGNEQF
                     ERALASRLLSPWLKACGMSADQLMELGRGLGDEMEPPFDMTAFVLRHATAVNAVSRLH
                     GETANATWASLRPQPISAITNGVHAATWIGRPLRRLFERATARSLGSDAPRPEVFDKL
                     AEVLETDLWGAHRQQKRELIEFMRSRLARQFARHGESPDVLRELHAAFDPETLIIGFA
                     RRFATYKRADLLFHDEHRLARLLADSERPVQLVLAGKAHPADRPGQQVIQRIFTLSRT
                     EPLRGRIFILEDYDMRIARFLVGGVDIWLNNPRRPMEASGTSGMKAAMNGIPSISILD
                     GWWDEGFNGRNGWAIGGREAVGDDAAQDEADAAELYRLLEDEVVPRFYARDGEGVPGD
                     WIDTMREAIAAAIWQFSTARMLGQYVEEMYRPAARAVEPATAGSA"
     gene            5468..>6253
                     /locus_tag="VIN32_03225"
     CDS             5468..>6253
                     /locus_tag="VIN32_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921624.1"
                     /translation="MDGYSIQEAASVLGVAEGRVWELLARGVLFGTPEGDSMRVFLKA
                     QPGPIASAGTRDEAPRTNGNGGSHGSAGEASAFRELLTEFRNLTERYGQALLALGEAR
                     GEVAGLRSRVDQLEARFDLRLPPPLEAAPVAWESTSGPDAAPEAPSPATRAPRRPRTP
                     VQRPPRPRRAPSARETVAGFADALARAQDPNAADLGGAAQTEAAVPDEPRDRTESPVQ
                     APDVGDAAPEALEAPEPAEQEPIAASDLAVEAPEADQAVAEPEG"
BASE COUNT         1002 a         2152 c         2161 g          938 t
ORIGIN      
        1 ggcatggtct tgaccgtgaa gtccgcgagc tcagcggtgg gaacttcgga ccagagcagt
       61 ggtgcggaga cgcgggcatc cggcactggc cgcaccgaat aggcggacgc caccgtgcgg
      121 tccttggcgt tctggttgta gtcgatgaag accccatgcc gctcctcctt ccaccacttg
      181 ctggtggcca ggtccggcac gcggcgctcg acctcacgag ccagggcaag ggccgatcga
      241 cgcacctcgc tgaaggtcca gcgtcgctcg atccgggcat agacgtggat cccccgtgac
      301 cccgacgtct tgggccagcc ggtcaggccg tggtcggcca ggacctcgcc cgccaccacc
      361 gccacgtcgc gcaccgcgct ccacggcacg cctggaccag gatccaggtc gacccgcagc
      421 tcatcgggat ggtcgaggtc gtcggcgcga agcgggtgcg ggttgaggtc gatgcagccg
      481 aggtttacga cccaggccaa tgcggcgccg ttgctgacca cgacctcgtc cgcagtgcgc
      541 ccggacggga acgagagctc gaccgtgcga acgaattcgg gaaccgtggc cggcgcccgc
      601 ttctggaaga aggcctcgcc ggccgcaccg tggacgaagc gcttgagcgc catcggccgg
      661 tccatcaccc cgcgcagcgc acccggcgcc acggcgaggt agtagttgac gaggtcgagc
      721 ttggtgaccc cggcctgctc gaagaagagc ttgcccggat tgctgatggc aacctcgaag
      781 ccgtcgatct cgagctggac cgatgcgtca gccggcacca cgacagggta gcctctcacc
      841 gatggtcgag cgctggaagc ggctccagga agcagcgaat ggctgggcgt cccggtggcg
      901 gaccacccga gtcgggcgcc gcgctctcgc gggcttcgcc ctgcacgacg gaccgagcgt
      961 ggccagcagc atggcctact tcgccatcct ctcgctcttc caggtcatcg tgctgggcgt
     1021 gatcgtgttc agcctgctga tcggtgaggg cgaggcgcga cgaatcgtga tcgggcgcct
     1081 cgagtcggcg ctgccgctgc agcccgggac ggtgacctcc gtggtcgaga gcatcatcgc
     1141 ggcgcgcggc ggagtgacca tcgtcagcgt tcccctgctg gcatggggag cgatagggtt
     1201 cttcggtgcg atgagcaccg gggtgggtcg tgccttcgcg acttcgacac ggcgcccgtt
     1261 ctggcaggac aagctgatcg cgctgctcct cctcgggggg tccggcctgc tgaccctgat
     1321 cgcggtcagc atcggcctgg gggagagcat cgccgctcgg cttgccgatc gcatcccggg
     1381 gggcggcagg ggcgggcagc tgctggtgga cctggtcggc ttcgcgctcc cgatcctgct
     1441 ggtgctggtc gccctgctcg tcatctatcg cgtcgttccg aaccggcgcg tcaccgtccg
     1501 ccaggtgctg cccggggcga tcgtggcgac catcctgttc acggtgctgc gctccggctt
     1561 cacctggtac gccaccgacg tggcccgcta cgagagcttc ttcggcccga tctcgaccgt
     1621 gatatcgctc ctcgtcttcc tccatttcgc cagcatggtg gtcctccttg gcgcggagat
     1681 cgcgcgcgcc aacgtgctcg aggatgacga agggctggac aaataggtgg actcttgaat
     1741 agaacgggtg gtacctttcg gccacgtcac gcgctctgca tgcgcggcta ggatcgccac
     1801 aggagcacgt agcgggacat ctgggccgat gaaacaagca cagacgattg tcgtatgagg
     1861 cgacgatctc acctggccat catggtgatt gccctcctga tggtcggaac gagcatcgtc
     1921 accacctccc ctcgcgacca ggcccaggcc gcggatccgc tcgccgaagc gaaggcgcag
     1981 cggcaggcgc tcgaggcaac tctgagcgca cagcgcgctg cgctcgccca gctcaggagc
     2041 aaggcctcga cgctggccgg aaagctggat gcagcgaggg cgcaactcgc atcggtgacc
     2101 gcggaatacg accacgtcag cgggctgctc gccgaggtgc aggccgaggt ggccgacatc
     2161 acggcccgcc tggctgacct gcgtgcgcag gtcgccagcc tcgacaagca gctccaggga
     2221 gtggcgatcg agatcatcgc ccagaccgac gagctccaca ctcgcgagtc gctcctccag
     2281 gaccacatgc gctctgccta tgagcgcagc cagacctctg tgctcgagct gctcctctcg
     2341 gccgattcgc tcgacgccgc caccaaccag gtcagctaca tgatcacggt ctccgaccag
     2401 gacaagcagc tggccatcga gatcagggac ctgcgggcaa gcctcgagac gaaggaagag
     2461 agcctccgcg ccggccgtct cgaggtcgcc gcggcgcgcg atcaggcgaa tgctgaggcc
     2521 aaggccctgg cgcagcgaga agccgagctg gcgacgatgc aggccgagct ggccaggctg
     2581 cagtcagccg ctgatcagaa gcgtgcggag caggaggccg cgctgaacgc cgccctccag
     2641 gcgaagggca atgtcgcgca gcagatcgca gacagccaga aggcgttcga cgcgcagacc
     2701 aagctcgtcg ccaagctcca ggccgaggag gatgcaaggc gccaggcgcc gtcggcattc
     2761 ggcttcaagt ggcccgagct cacgttccag gtcacacagg agtggggccc cacaggattc
     2821 gtgctcgagc cgtcgtacac ctacaacggc acctactact cgcacttcca tggcgggatc
     2881 gacatcggca acggctgcgg aacgcccatc ctggctgtcg gagacgggac ggtccgtgca
     2941 tccgggcagc cgctgtggcc gtgggatacg gggtacgggg tggtcatcag ccacggctcc
     3001 ggcgtccaga gctggtactg gcacctgcgt gctcaggtca tcgtcagccc tggccaggtg
     3061 gtcagccgcg ggcaggtgat cgggtacgag ggcaacaccg gtaactcgac cggctgccat
     3121 ctccacttcg cgatcaacga ccacggcatc tgggagaacc cgcgggtcta cctgccctag
     3181 gccgatcccg cggtcgcggg ctcgaccgcc cgcgcagccg ggcgatacat ctcctcgacg
     3241 tactggccga gcatgcgggc cgtgctgaac tgccagatcg ccgccgcgat cgcctcgcgc
     3301 atggtgtcga tccaatcgcc gggcacgccc tcgccatccc gcgcgtagaa gcgcggcacg
     3361 acctcgtcct cgaggagccg atacagctca gccgcatcgg cttcgtcctg ggcggcgtcg
     3421 tcgccgaccg cctccctgcc gccgatcgcc cagccgtttc gcccgttgaa cccctcgtcc
     3481 caccacccgt cgaggatgct gatcgagggg atcccgttca tcgccgcctt catgccggag
     3541 gtgcccgatg cctccatcgg gcggcgtggg ttgttcagcc agatgtcgac cccacccacc
     3601 aggaagcggg cgatccgcat gtcgtagtcc tcgaggatga agatccgacc gcgcagcggt
     3661 tcagtgcgag atagcgtgaa gatgcgctgg atgacctgtt ggccggggcg gtccgcaggg
     3721 tgcgccttgc ccgctaggac cagctgcacc gggcgctcac tgtcggccag cagccgcgcc
     3781 aggcggtgct cgtcgtggaa cagcaggtcg gctcgcttgt aggtcgcgaa gcgccgagcg
     3841 aagccgatga tcagtgtctc cggatcgaag gcggcatgca gctcgcggag cacgtccggg
     3901 gactcgccat gccgcgcgaa ctggcgggcg aggcggctgc gcatgaactc gatcagctcc
     3961 cgcttctgct gccggtgggc gccccacagg tccgtctcca gcacctccgc cagcttgtcg
     4021 aacacctccg gccggggtgc gtcgctgccc agcgagcgag cggtggcgcg ctcgaagagg
     4081 cgacgcaacg ggcgcccgat ccaggtcgcg gcatggaccc cgttggtgat ggctgagatg
     4141 ggctgcggcc gcaacgaggc ccaggtggcg ttggccgtct cgccgtgcag gcggctgacc
     4201 gcattgacgg cggtggcgtg gcgcagcacg aacgcggtca tgtcgaaggg tggctccatc
     4261 tcgtcgccga ggccacgacc gagctccatg agctggtcgg cggacatgcc gcaggccttc
     4321 agccaggggc tcagcagcct gctggccagg gcgcgctcga attgctcgtt cccgtccttg
     4381 accggggtgt gaatggtgaa aacggagttc tggccgacgc gccgcagcgc ctcatccgct
     4441 ccgaggccgg gctccgctgc aaccagctca cgggcgcgtt cgaggagcag gaatgcggaa
     4501 tgtccctcgt tcaagtgcca tgcggccggc tcgatgccga gcccgcgcag ggcccgcacg
     4561 ccgccgatcc cgagcacgat ctcctggcag agccgcatct cgcgaccgcg gacgtagagg
     4621 atgtgggtga tcggccggtc gctcccctcg ttggcgggga tatcggtgtc cagcaggagc
     4681 agcggcacgc gacccacctg cgccagccag accgcggcgt ggaccttgcg atccgcgaag
     4741 tcgacgctca cctgtagcgg cgagccgtcg cgcccgcgcg ctcgacgcag cggcagcatg
     4801 gcggggtcaa ggtccggctg agcgtgctcc tggtggccgt ccgcatcgat ctgctggcgg
     4861 aagtagccgc gccgatacag caggccgaca cccacgaacg gcaacgccgc gtcggaggcc
     4921 gacttgcaat ggtcgccggc gaggatgccg agcccgccgg agtagatctg catcgactca
     4981 tggatgccgt actcggcaca gaagtacgca atcggaccag ggagggtcgc agacgggtcc
     5041 gagtccgtcg ctgcccgata ccaggagtcc gacccgttgg cgaggtagcg ttcgaactcg
     5101 tccaggacgc ggctggcatc caccatgaag tcctcgtcgg ccagcagctc cgtccatcgg
     5161 cccggctcga ccgcggcgag gacggggatc gggctccggt agcgcgacca ggccgcggaa
     5221 tcgatccgcg caaagagcgc ccccgcccgt ggggtccagc tccaccacag gttgtacgcg
     5281 agttcgccca ggccttcgag cgcgggcggg agcccgaaat cgtagcctgg ctgactgggc
     5341 acccggaccg cggacgtcat gagcggcccg atcataggca gtcacccccg cgctccgtcg
     5401 gtcctcgcgc caaaacgagc ggggtatcga tgtttttggt gtcagggcgt accctatcgg
     5461 acccgtgatg gacggctact cgatccagga ggcagcgtcg gtcctcgggg tcgctgaagg
     5521 gcgcgtctgg gagctgctcg ccagaggcgt gctgttcggc actcccgagg gtgactccat
     5581 gcgcgttttc ctcaaggcac agcccggacc gatcgcctcg gcgggcacgc gcgacgaggc
     5641 tccacgcacg aacggcaacg gcggctcgca cggctcggcg ggggaggcga gcgcatttcg
     5701 cgagctgctc accgagtttc gcaacctgac cgagcgatat ggccaggcgc tgctggcgct
     5761 gggcgaggcg cgtggtgagg tggcgggact ccgttcgagg gtcgaccagc tcgaggcccg
     5821 cttcgatctg cgcctgccgc cgcccctgga agcggccccg gtcgcgtggg agtcgaccag
     5881 cggtcccgat gccgcgccgg aagcgccctc accggccacg cgagctcctc gacggccgcg
     5941 tactcccgtg caacgtccgc cccggccgcg ccgcgccccg tcggcgcggg agacggtcgc
     6001 aggcttcgcg gatgcgcttg cgcgagcgca ggatccgaac gcggcagacc tgggaggcgc
     6061 cgcccaaacc gaggctgccg tccccgacga gccacgcgac cggaccgaga gccccgtcca
     6121 ggcgcccgac gtcggcgacg ctgcgcccga ggcccttgag gcgcccgaac cggccgagca
     6181 ggagcctatc gctgcatcgg accttgccgt cgaagccccc gaagccgatc aggcggtcgc
     6241 cgagccagag ggg
//
LOCUS       DATCQR010000096         2779 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_206760, whole genome shotgun sequence.
ACCESSION   DATCQR010000096 DATCQR010000000
VERSION     DATCQR010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2779
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206760"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..450)
                     /locus_tag="VIN32_03230"
     CDS             complement(<1..450)
                     /locus_tag="VIN32_03230"
                     /inference="COORDINATES: protein motif:HMM:NF013328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HEY8921625.1"
                     /translation="MPELPEVETVARDLQRWVAGATIAETEVRWERTIRHPQPGERFA
                     AELRGATISRVGRRAKSVLLHLADGRVMTVALRMTGALIVAAPGAPDDRYARVVFRLA
                     DGRELRYRDVRKFGRIGLWERGGVPRPGRRGTRRRVAEAREPYRVGDV"
     gene            complement(457..>2779)
                     /gene="polA"
                     /locus_tag="VIN32_03235"
     CDS             complement(457..>2779)
                     /gene="polA"
                     /locus_tag="VIN32_03235"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEY8921626.1"
                     /translation="IPGVPGVGEKTAVSLLQQFGSLDNLYEHLDEVKGKLRERLAEHR
                     ESAFLSRKVGRIVTDLPVPLDLESARTGRYDRRAVAQRLRELEFRSLIDRLPPSSIAP
                     TGHDAPERDTKGGLQLSLDLLGGGSAPAPAGGEAGEGPSSRAAPDPLVDPGGAIEHVD
                     PRIPRSEADRIELEAWLAAHGDAVGLGWAAGPGRTHDRALLGIAMAGADGSCWYLPLD
                     PDPQAAPWLPHWFEREDRTLIGHDLKQVMVPLAKSGTTLHGAVFDTLVASYMLNPALR
                     AQTLDDLAANRYGTELPPRPVSGESGAEEVVVARRAAGEALIALLLKPALEAEMAEAG
                     LTALFSEVEMPLLPVLAEMELAGVAIDRAVLAAMAEEFGGRLASLEARIYELVGHPFN
                     IGSPKQLEQILFHELELPAAKKTRTGFSTDASVLEELRDKHEVVGLILEHRQVAKLKS
                     TYVDSLPLLADDEGRVHTTYSQAVAATGRLSSTDPNLQNIPIRTALGRRIRGAFVAPP
                     GKLLLGADYSQQELRILAHVSGDEGLKADFAAHNDVHLAAAARVLGLTPEQVGPRERS
                     VAKMINYGIAYGLSGFGLAQRLRIPQEEADRYIADYYAAYPGIRRYTVEMRILARDQG
                     FVSTLLGRRRYLPELTARNSALRSAGERMAINMPIQGTAADGIKIAMVHLQAAMRERG
                     LHSRMLLQVHDELVFETDPEELPVLAELAREVMEGALPLDPALEVDFKVGTNWQEMDR
                     YLRDDGAWRRVPKTSTEVAREEAEEAIAEPIGA"
BASE COUNT          382 a         1013 c          961 g          423 t
ORIGIN      
        1 gacgtcgccg acccggtacg gctcgcgcgc ctcagccacg cggcgcctgg tgccacgtcg
       61 tcccggtcgc ggcacgccgc cgcgctccca gagcccgatc ctgccgaact tgcggacgtc
      121 ccggtagcgc agctcgcggc cgtcggccag gcggaatacg acgcgcgcgt agcggtcgtc
      181 cggcgccccc ggcgcggcca cgatcagcgc accggtcatg cgcagggcaa cggtcatgac
      241 ccgcccgtcg gcgaggtgca ggaggacgct cttcgcccgt cgcccgaccc ggctgatggt
      301 cgcgccgcgc agctctgccg cgaaccgctc accgggctgc gggtggcgga tcgtccgctc
      361 ccagcgcacc tcggtttccg cgatcgtggc gccggccacc cagcgctgca ggtcgcgggc
      421 cacggtctcg acctcgggga gctccggcat caggcctcac gcaccgatcg gctcggcgat
      481 ggcctcttcg gcttcctcgc gcgccacctc ggtcgacgtc ttgggaacgc gccgccaggc
      541 cccgtcgtcg cggaggtatc ggtccatctc ctgccagttc gtcccgacct tgaagtcgac
      601 ctcgagtgcc ggatcgagcg ggagggcccc ctccatcacc tcgcgtgcca gctcggcgag
      661 cacgggcagc tcctcgggat cggtctcgaa cacgagctcg tcgtggacct ggagcagcat
      721 ccgcgagtgg aggccacgct cccgcatcgc cgcctggagg tggaccatgg cgatcttgat
      781 accgtccgcc gccgtcccct ggatcggcat gttgatggcc atccgctctc ccgctgaacg
      841 gagggcgctg ttccgcgccg tgagctccgg aaggtagcgt cgccgtccca gcagggtgct
      901 gacgaagccc tggtcgcggg ccaggatccg catctcgacg gtgtagcggc ggatccccgg
      961 gtaggcggcg tagtagtcgg cgatgtagcg gtccgcctct tcctgcggaa tgcgcaggcg
     1021 ctgcgccagg ccaaagcccg acaacccata cgcgatgccg tagttgatca tcttggccac
     1081 gctgcgctcc cgcgggccga cctgctcggg cgtcaggccc agcacgcgtg ccgcggccgc
     1141 aaggtggacg tcgttgtgcg ccgcgaagtc ggccttcagg ccctcgtcgc cggagacgtg
     1201 ggccaggatg cgcagctcct gctgcgagta gtcggcaccc agcaggagct ttccgggagg
     1261 cgccacgaag gcaccgcgaa tgcggcggcc gagcgccgtt cgaatcggga tgttctggag
     1321 gttcggatcg gtgctggaca gccggcccgt cgccgccacc gcctgtgagt aggtggtgtg
     1381 cacgcggcct tcgtcgtcgg ccagcagcgg cagggagtcg acgtaggtgg acttcagctt
     1441 agccacctgg cgatgctcca ggatcagccc gaccacctcg tgcttgtcgc ggagctcttc
     1501 caggaccgat gcgtccgtcg agaacccggt gcgggtcttc ttggcggcgg gcagctccag
     1561 ctcgtggaac aggatctgct cgagctgctt gggcgagccg atgttgaacg ggtggccgac
     1621 gagctcatag atccgcgcct cgagcgacgc gagccgcccc ccgaactcct cggccatcgc
     1681 cgccaagaca gctcggtcga tcgccacccc cgccagctcc atctcggcga gcaccggcag
     1741 gagcggcatc tcgacttcgc tgaagagggc ggtcagcccc gcctccgcca tctcggcttc
     1801 gagcgctggc ttcaggagca gggcgatcag cgcctcgcca gccgcgcgac gggcgacgac
     1861 cacctcctct gcgccggact ccccgctcac cggtcgcggt ggcagctcgg tcccgtaccg
     1921 attggccgca aggtcgtcga gggtctgggc gcgcagcgcc gggttgagca tgtaggaggc
     1981 aaccagggtg tcgaagaccg cgccgtgcag ggtggttccg gacttggcca ggggcaccat
     2041 cacctgcttc aggtcgtgcc cgatcagcgt ccggtcctcc cgttcgaacc agtggggaag
     2101 ccatggcgct gcctgggggt cggggtcgag gggcaggtac cagcacgatc cgtcggcccc
     2161 ggccatggcg atcccgagca gggcccggtc gtgggtgcgc cccggacccg ccgcccagcc
     2221 gaggccaacc gcgtctccgt gtgcggcgag ccaggcctcc agctcgatcc gatccgcctc
     2281 gctgcgcgga atgcggggat cgacgtgctc gattgcgcct cccggatcga caagtgggtc
     2341 cggcgccgcg cgcgaggacg gcccctcacc cgcctcaccg cccgctggcg caggtgccga
     2401 accgccgccc agcaggtcga gcgacagctg gagtcctccc ttcgtgtcgc gctccggcgc
     2461 gtcatgcccc gtcggcgcga tgctcgatgg cggcagccgg tcgatcagcg agcggaactc
     2521 gagctcgcga aggcgctgtg caaccgcgcg gcggtcgtag cggcccgtgc gcgccgactc
     2581 gagatcaagg ggcaccggca ggtcggtcac gatgcgtccc accttccgcg acaggaaggc
     2641 ggactcgcgg tgctccgcca gtcgctcgcg cagcttgccc ttcacctcgt cgaggtgctc
     2701 gtacaggttg tcgagcgagc cgaactgctg cagcagcgac accgccgtct tctcccctac
     2761 ccctggcact ccagggatg
//
LOCUS       DATCQR010000097         4720 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_144942, whole genome shotgun sequence.
ACCESSION   DATCQR010000097 DATCQR010000000
VERSION     DATCQR010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4720
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144942"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..653)
                     /locus_tag="VIN32_03240"
     CDS             complement(<1..653)
                     /locus_tag="VIN32_03240"
                     /inference="COORDINATES: protein motif:HMM:NF014448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HEY8921627.1"
                     /translation="MQVTIRCFATLRELALDRTTLTLSPGAVVADAWAALAQAYPGLV
                     PHRPFVRAARNGAYAGWEVGLEDGDQIAFLPPVSGGGPGGLTDGVIDIGSLEASVAAT
                     GHGALVTFIGRARDRGDDGREVLELEYEAYPEMATDVLTEIVAEAEDRWRGCAVAVMH
                     RVGVVPIGGAAVAIVTAAPHRSEAYEASRFVIEAIKERLPIWKRERFADGTDWKRPGA
                     "
     gene            complement(662..1441)
                     /locus_tag="VIN32_03245"
     CDS             complement(662..1441)
                     /locus_tag="VIN32_03245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921628.1"
                     /translation="MDLEALPFVNWIFWAALTSGSLLVVGATELLGGTTRGYRLFMAG
                     LLAVCAAVLLLSELNLSPGTVADETAGIRRGLVWAVTALTAAYLGASIANVPRSGLAL
                     AAGSVGIFSLAALAAAGGTLSAPLFTAQLMLISVALGAVTAAMLLGHWYLVTPKLSPA
                     PLRRMMWVLLAALVMQAVLFVVAVTAISSGPLGGPIGWLTWLRLVAGILLPIGITVLA
                     LLASRAASLQASTGLLYIGLALVMAGSIAGASLAYLTGVPV"
     gene            complement(1452..1820)
                     /locus_tag="VIN32_03250"
     CDS             complement(1452..1820)
                     /locus_tag="VIN32_03250"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HEY8921629.1"
                     /translation="MTDLAAAPAGAAIRYARVLPASELRDGELIPVEIDSRPVVLVRH
                     AGAFYAVQNNCSHKDFPLSEAGFDPRDEVLVCAWHGGCFDLRTGEAVVPPATEPVETF
                     PVRVTADGWVEIGLTGSLAS"
     gene            complement(1827..3269)
                     /locus_tag="VIN32_03255"
     CDS             complement(1827..3269)
                     /locus_tag="VIN32_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011396075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY8921630.1"
                     /translation="MSTQVTSETVPIFLAGEFVDAGTPLEVRDPATGELVATTWQAGP
                     EELERATIAAVEAFAATRRLASFERRDALAHVAERISQDADELAELLSRESGKPIRDA
                     KGEVARGSLTFRIAAEEALRINGEWLPLDWSAANRGRSGIVRRYPIGPVAGISPFNFP
                     LNLAAHKVAPAIAAGCSIVLKPPSKDPLVMLRVAGYLAETNLPKGAVSILPMDRPTGD
                     RMVADDRFKLLSFTGSPSVGWKMKAEAGKKKVVLELGGNAGAIVDDTADLEWAVARLA
                     YGAFAYSGQVCISVQRIYVVESIFADFQRLFVEKVRQLKVGSPLDPATDLGPMVNARA
                     VARTHEWVGEALERGAKALVGGEPDGLFYPPTVLVDVPKDARVCGEEVFAPVVNLFPV
                     PDFKAAIAELNDSRFGLQCGVFTNDLERTLLAHDELEVGGVIVNDVPTWRTDSMPYGG
                     VKDSGLGREGLRWSIEDMTEPRLLAFARPL"
     gene            3316..4266
                     /locus_tag="VIN32_03260"
     CDS             3316..4266
                     /locus_tag="VIN32_03260"
                     /inference="COORDINATES: protein motif:HMM:NF016155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid biosynthesis protein"
                     /protein_id="HEY8921631.1"
                     /translation="MNPLYLLIEASASIFFIGAAVAALRRGRLPFLELISAAAFGILL
                     EEGDQLVFETYHYSANWILTIDRAPVVIGLTWALIIAGAMRLTDALGVRRRYAPFVDA
                     VLAISLDLAFDAIAIRMGLWTWRGIGQSEGWFGVPAGNFYSWLVVTLAFSSLTRWLRD
                     ASARRRALEWLQLAVPLPAFGLLLAGIVPFAVLQPMIDPRPGGGMLLFVIVFVGFLAV
                     ASWAVWGPDRARPNGDRLAILDLGLALGTRVAIYGFFITALVLIGAAWRQPVLLIAAV
                     VLLAAEWPLGRLVHARHTAAHASMQRALPDSGQAPAQSYR"
     gene            4272..>4720
                     /locus_tag="VIN32_03265"
     CDS             4272..>4720
                     /locus_tag="VIN32_03265"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEY8921632.1"
                     /translation="MARDATTVDWARASAECVEHLRALIRIPSVNPPGVADAAAGRDS
                     TGGETAAAAYCAEVLTSAGVAAEVLETVPGRGSCFARLPATLPDPDPPLVLLSHLDVA
                     PVDVESWSQDPFGGELIDGVVWGRGAVDMKDMVAMELSVMLALARDGG"
BASE COUNT          695 a         1687 c         1613 g          725 t
ORIGIN      
        1 gcgcccgggc gcttccagtc ggtcccgtct gcaaatcgct ctcgcttcca gatcgggagg
       61 cgctccttga tcgcctcgat gacgaaccga ctggcctcgt acgcctccga gcgatgcggc
      121 gccgccgtga cgatcgcaac cgccgccccg ccgatcggca ccacgccgac ccgatgcata
      181 acggccacgg cgcagcccct ccatcggtcc tcggcctcag cgacgatctc ggtcaggacg
      241 tcagttgcca tttcggggta ggcctcgtac tcgagctcca gcacctcgcg gccgtcatcg
      301 cctcgatctc gggcccgccc aatgaaggtc accagcgccc cgtgaccggt cgccgccacc
      361 gatgcctcca ggctcccgat gtcgatcacg ccgtccgtca ggccgcctgg tcccccgccg
      421 ctcaccggcg gcaggaatgc gatctggtct ccatcctcga gcccaacctc ccagccggcg
      481 taggcgccat tccgtgcggc acgcacgaac gggcgatgcg gcacgagccc ggggtaggcc
      541 tgcgccagcg ccgcccatgc gtcggccacc acggccccgg gtgagagggt cagcgtcgtc
      601 cggtccagcg ccagctcgcg caaggtggca aagcagcgga tcgtgacctg catcgcgatc
      661 gtcagactgg gacgccggtc aggtaggcga ggctcgcgcc ggcgatcgat cctgccatca
      721 ccagcgccag gccgatgtac agcagcccgg tgctggcctg gagggacgct gcgcggctgg
      781 ccagcagtgc gaggaccgtg atcccgatcg gcaggaggat gcctgccacc aggcgtagcc
      841 aggtgagcca cccgatcggt ccacccagcg gcccgctgct gatcgcggtg acagcaacga
      901 cgaagaggac ggcctgcatc acgagcgcgg caagcagcac ccacatcatg cgtcgcagcg
      961 gggccggtga gagcttcggc gtgacgaggt accagtgccc gagcagcatt gcggcagtga
     1021 cagctccaag cgccaccgag atgagcatca gctgtgcggt gaagagtggc gcgctgagcg
     1081 ttccgccggc cgcggcaagg gcggcaagcg agaagatccc gaccgagccg gctgccagtg
     1141 ccaggccgct gcgcggcacg ttcgcgatgg atgcccccag gtacgcggcc gtcagggccg
     1201 ttacggccca gaccaggccg cgacggatcc ccgccgtttc gtcggcgacc gttcccggcg
     1261 acaggttcag ctcggagaga agcagcaccg ccgcgcacac ggcaagcagg ccggccatga
     1321 agagccgata gccgcgcgtc gtgccgccca gcagctcggt tgccccgaca acgagcagcg
     1381 accccgaggt gagggccgcc caaaagatcc agttgacgaa agggagcgcc tcgagatcca
     1441 tcgatcgtcg ctcagctagc gaggctgccg gtgaggccga tctcgaccca gccatcggcg
     1501 gtgacccgca ccgggaacgt ctcgaccggc tcggttgccg gcggaaccac cgcctcgccg
     1561 gttcgcaggt cgaagcagcc gccgtgccag gcgcagacca gcacctcgtc gcgtgggtcg
     1621 aagcctgcct cagacagggg gaagtccttg tgcgagcagt tgttctgcac cgcatagaac
     1681 gctcctgcgt ggcgcaccag cacaacgggg cggctgtcga tctcgaccgg gatcagctct
     1741 ccgtcgcgca gctccgaagc gggcagcaca cgggcatagc ggatcgcggc acctgccggc
     1801 gctgcggcca gatcggtcat ggcggcctac agcggccggg cgaacgccag caggcgcggc
     1861 tcggtcatgt cctcgatcga ccagcgcagg ccctctcgac ccagccccga gtccttgacg
     1921 ccgccgtacg gcatcgagtc ggtgcgccag gtcggcacgt cgttgacgat cacgcctccg
     1981 acctccagct cgtcgtgcgc gagcagcgtg cgctccaggt cgttggtgaa tacgccgcac
     2041 tgcaagccga atcggctgtc gttgagctcg gcgatcgccg ccttgaagtc gggcaccggg
     2101 aagaggttga cgaccggagc gaagacctcc tcgccgcaga cccgcgcgtc cttcggcacg
     2161 tcgaccagca cggtcggggg gtagaagagc ccgtccggct caccgccgac gagcgccttc
     2221 gccccacgct ccagtgcctc gcccacccac tcgtgcgtac gggccacggc tcgggcattg
     2281 accatcggcc cgagatcggt cgccgggtcg agcgggctgc ccaccttcag ctggcgcacc
     2341 ttctcgacga agaggcgctg gaagtcggcg aagatcgatt ccacgacgta gatgcgctgc
     2401 acgctgatgc agacctgccc ggagtaggcg aaggcgccgt acgccaggcg cgccacggcc
     2461 cattccagat ccgctgtgtc gtccacgatg gctccggcgt ttccgcccag ctccaggacc
     2521 accttcttct tgccggcctc ggccttcatc ttccagccaa ccgatgggct gccggtgaag
     2581 ctgaggagct tgaaccgatc gtcggcgacc atccgatcgc cggttggacg atccatgggc
     2641 aggatcgaga cggccccctt gggcaggttc gtctcggcca ggtagccggc cacacggagc
     2701 atcaccagcg ggtccttgga cggcggcttg aggacgatgc tgcaaccagc cgcgatcgcg
     2761 ggcgcgacct tgtgggccgc caggttcaac gggaagttga atgggctgat gccggcgacg
     2821 ggaccgatcg ggtagcggcg cacgatcccg cttcgccccc ggttggccgc gctccagtcg
     2881 agcggcagcc actcaccgtt gatgcgcagc gcctcctcag cggcgatgcg gaacgtcagt
     2941 gagccgcgcg ccacctcgcc cttcgcgtcg cggatcggct tgcccgactc ccggctgagg
     3001 agctcggcca gctcgtccgc atcctggctg atccgctcgg cgacgtgcgc cagggcgtcg
     3061 cggcgctcga agctcgccag gcgccgcgtg gctgcgaagg cctcgaccgc tgcgatcgtg
     3121 gcccgttcga gctcctcggg tccggcctgc caggtggtcg ccaccagctc gcccgtggcc
     3181 gggtccctca cctcgagcgg cgttccagcg tcgacgaact cgccggcgag gaagatggga
     3241 accgtctcgg aggtcacctg cgtgctcacg gcggcattct acgaccggcc ggccgcggcc
     3301 tcgtatcatc ggagcgtgaa cccgctctac ctgctgatcg aggcctccgc ctcgatcttc
     3361 ttcatcggcg cagcggtggc ggcgctgcga cgcggacgcc tcccgttcct ggagctcatc
     3421 tcggcggccg ccttcgggat cctgctggag gagggcgacc agctcgtctt cgagacgtac
     3481 cactactcgg ccaactggat cctgacgatc gaccgggcgc cggtcgtcat cggcctgacc
     3541 tgggcgctca tcatcgccgg cgccatgcgc ctgaccgatg cattgggcgt gcgtcggcgg
     3601 tacgcgccgt tcgtcgacgc ggtgctcgcg atcagcctcg acctcgcctt cgacgccatc
     3661 gcgattcgga tgggcctttg gacctggcgt gggatcggtc agtccgaggg ctggtttggc
     3721 gtgccggcgg gcaacttcta ttcgtggctg gtggtgaccc tggccttcag cagcctgacc
     3781 aggtggctgc gtgacgcctc ggcgcgacgg cgcgcgctcg agtggctcca gctcgctgtc
     3841 ccgctgccgg ccttcgggct cctgctggcc ggcatcgtgc cgtttgccgt cctgcagccg
     3901 atgatcgacc cgcgacccgg aggagggatg ctgctgttcg tgatcgtctt cgtgggcttc
     3961 ctggcggtcg cgagctgggc cgtttggggt cccgaccggg cgcgaccgaa cggcgaccgg
     4021 ctggcgatcc tcgatctcgg cctggcattg ggcacccggg tggccatcta tggcttcttc
     4081 atcaccgcgc tggtgctgat cggcgcggcc tggcggcaac cggtcctgct gatagcggcg
     4141 gtggtcctgc tggcggcgga atggcccctc gggcgtctgg tgcatgcccg tcacaccgcg
     4201 gcccacgcct cgatgcaacg agcgctgcct gacagcggcc aggcgccggc gcagagctac
     4261 cgctgagcaa catggccaga gacgcgacga ccgtcgattg ggcgcgggca tccgccgagt
     4321 gcgtggagca cctgcgggca ctcatccgga tcccgagcgt gaacccgccc ggcgtggccg
     4381 acgcggctgc gggcagggac tcgacgggcg gcgagacagc ggccgccgcc tattgcgcgg
     4441 aggtgctcac ctcggccggc gtggccgccg aggtgctcga gacggtgccc ggccgcggtt
     4501 cgtgcttcgc ccgcctgccc gccaccctcc cggaccccga tccgccgctc gtcctcctct
     4561 cacatttgga cgttgccccg gtcgacgtcg agtcgtggag ccaggacccc ttcggcggtg
     4621 agctgatcga cggcgtggtg tggggtcgcg gcgcggtcga catgaaggac atggtggcga
     4681 tggagctcag cgtgatgctg gcccttgccc gcgacggcgg
//
LOCUS       DATCQR010000098        16879 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_145097, whole genome shotgun sequence.
ACCESSION   DATCQR010000098 DATCQR010000000
VERSION     DATCQR010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 16879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16879
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145097"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="VIN32_03270"
     CDS             complement(<1..330)
                     /locus_tag="VIN32_03270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921633.1"
                     /translation="MNPMATVRRLTAVAVLTMFVGCASPSPSLVPSPSAIPTPSPSPT
                     ATPIPDVTFPLAVVTGLTNLKATITLDELGTLASGGQLVVPCEVTLTEPAITATAPCV
                     AANQVAVD"
     gene            complement(358..1341)
                     /locus_tag="VIN32_03275"
     CDS             complement(358..1341)
                     /locus_tag="VIN32_03275"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEY8921634.1"
                     /translation="MTPATGRPSTLAVAAALGTVYVLWGSTYLAIAFMIESLPPLLAA
                     GIRFLTAGLTLVAFVVARARWRRRRGLTGEALVRPRAVEWRTAFIVGALLLLGGNGMV
                     SIAEQTIPTGIAAVIIATTPIWLSLFDALLTRRMPSLLAIGGLLVGLGGVAILLLPSS
                     GLDSFDPIGIGILVVAAVSWASGSLYARRGPLPRNQLLGTGMEQLAGGLVLLAAGVAI
                     GELGRFDPGAVTTASLLGLAFLIVFGSLVAFTAYIWLLNHVAVTTVATYAYVNPVVAV
                     ALGVAFRGETLTPRSLLAAALIIGAVVAMVSGRPRKIEEPGAPSGRVAPST"
     gene            1474..1818
                     /locus_tag="VIN32_03280"
     CDS             1474..1818
                     /locus_tag="VIN32_03280"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEY8921635.1"
                     /translation="MKYMLIIAGADDAWGHLSEAEQGALYEKVRAWWGEHSASGEILD
                     GHELQPGSTATTIRRDPSGEVTVTDGPFVEGKEMVAGYGILDVPDLDAAIMLASSWPA
                     PDTLEIRPIVER"
     gene            complement(1829..2989)
                     /locus_tag="VIN32_03285"
     CDS             complement(1829..2989)
                     /locus_tag="VIN32_03285"
                     /inference="COORDINATES: protein motif:HMM:NF044870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921636.1"
                     /translation="MLAITAGLLLAACSTTSPSPSSSPSGSALASPSAGLPSVAPSLP
                     AEPSPTPIGGVYAATTSGQIDPSLAGVPERVYVPNELSGDVTVIDPATFAVVGTLTTG
                     AYPEHVTPDWDLKRLLVSNMNGGSLSVVDPRTAQAVDSIGLSIFPYAVYFTVDGQKAM
                     VVTDYISPSLVKDNGVTFYDRGSWELLKFVQVPWPGADDLDLSADGSYLMISCEYSGQ
                     LAKVDTSAMALTGNVEVGSLPRDVRLAPDGRSFFVANEGLDGVQVVDPEAMAVTTFIP
                     TGDGAHGLEFSRDTSQMFVANRAAATISVIDLASLKVVATWSVGGSPDEMVLSPDGSQ
                     LWASNRYHRSVSVIDTQTGEVLTTIPVGASPHGLTYWPQPGRMSIGQNGNMR"
     gene            complement(3021..4199)
                     /locus_tag="VIN32_03290"
     CDS             complement(3021..4199)
                     /locus_tag="VIN32_03290"
                     /inference="COORDINATES: protein motif:HMM:NF044870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HEY8921637.1"
                     /translation="MHTRRVTTLLITGIMLAACSATGQSATPGASSGAVPSDAPNATP
                     LPTPTVEPTPTATPPGGVYSGTVGGQLDPRVADLPLRVYVPNELSNDVAVIDPQTLTV
                     IGRFDVGAAPEHITPDWDLSKLYVNNMNGASLTVIDPLTEKPSGTQQVPFPYNLYYTP
                     DGSKAIVVADYLGMGMIADNGLYFYDRETWKLLKFVQVPWPGVNHLDFSPDGSFLVVS
                     CESAGVVAKINTQTMTVEGSVKLGGSPLDVRLAPAGDVFFVANQGTNGVDVVDALSME
                     RVDFIPTAKGAHGFALSRDVTRLFVMNRLAGSISVIDVATRKVVDTWEVGGSPDMAAL
                     SPDGTQLWVSNRFHGTVMVLNPETGEVIKVIPTGGNPHGLTFWPQPGTISLGHNGNMR
                     "
     gene            4301..5551
                     /locus_tag="VIN32_03295"
     CDS             4301..5551
                     /locus_tag="VIN32_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020575413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEY8921638.1"
                     /translation="MTERHAVDSSLDLVLREERTRLIASLVRILGDWDLAEELVQEAS
                     VSALEHWPIDGLPRNPGAWLMTTARRRAIDRLRRDARFRQRLEQLGAEAEEMEQPIAT
                     GGPLAADDRLRLLFTCCHPALSQDAQVALTLRTVGGLDTTQVARAFLVPESTVAQRLV
                     RAKRKIRDAGIPFRIPEASELPGRLQGVLAVLYLVFNEGYLASDGAAADRRDLARDAE
                     WLTSLLVRLMPDEPEPLGLLALMRLHLARIEARFAPDGSLILLPDQDRSRWDGSAIAD
                     AGDLVERALRMGRPGPHLLQAAIVAVHATAPSYAATDWAEIVALYDRLFTLQPTPVVA
                     LNRALASAEVSGPEIALLEIEPLVTRLDGYHLFHAARGELLRRLGRLDEARSADRRAL
                     SLTDNPAERRLLEERLRHPTADGA"
     gene            5594..6226
                     /locus_tag="VIN32_03300"
     CDS             5594..6226
                     /locus_tag="VIN32_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921639.1"
                     /translation="MQLSRPALGGLTLIVSLALAGCAAGSPSVAPSDSPIASPSAAPS
                     TTASEAASVEASAAASEPTESLPAFVCTPSATIASTTNRAQITDVRVGTHDGYDRVVF
                     EFASGLPDAVIEGVLPPMYADPSGIEMDVAGTAFLRVTMHGATKLSPEGGLTYGGSTS
                     FKPGFDRLAQLIEGGDFEAVSTWYLGLNGAGCFRVLALSDPSRLVIDIEH"
     gene            6260..7780
                     /locus_tag="VIN32_03305"
     CDS             6260..7780
                     /locus_tag="VIN32_03305"
                     /inference="COORDINATES: protein motif:HMM:NF025067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4173 domain-containing protein"
                     /protein_id="HEY8921640.1"
                     /translation="MDSAPAVVRASAGIGLVALASGLLAQFLFVDAAFGINVLIATLA
                     VLLAGWVVRDGPTPRPRLLDGWLAPGALILAAFVSLRGDGTLVALDVVGAIALSGAAL
                     VSFGGLRVLERPFAAIAGLGARLALSGIVGGVGPFAALARMTSTLRGRSRPGRFVPVL
                     RGLLIAVPLVLVFVALFSSADAVFARITGDLLVWNLDLGSLPSRLALAAIVAWVAAGL
                     FAFVARGEDRPLGTTTDAVASDRPRLGSAEAVTVLVVLDLLFIGFVVLQGAYLFGGRD
                     TLDASGITYAEYARRGFFELLAVAFLVAGLILALEGFVRTRTRTYVLTAIGLVLLTIV
                     VLASAFLRLRLYQDAYGWTELRFYVLAAIAWLAIGAVIATVTLARNRTRWLIHGMLVL
                     SFVFGLFFNVIGPVKFIAEQNIQRAIHPELVAAGGETGLDVYYLAILGDDALSVMAEN
                     LCALPGPVAKQALEAVTMWRRFLGRDPAGKAWQAWNLSRERARDTRLQPVCLLSSR"
     gene            7841..8347
                     /locus_tag="VIN32_03310"
     CDS             7841..8347
                     /locus_tag="VIN32_03310"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY8921641.1"
                     /translation="MADRLAAPSGTLDLTKSYSARFKTERGEFTSELFAADAPMTVEN
                     FVNLARAGFYDGTTFHRVIPGFMAQAGDPTGTGTGGPGYSFRDELSVKRRHEGPGTLS
                     MANAGPNTNGSQFFITFGATPHLDGRHTVFGRVTSGMDVVNSIRERDPQRDSQPGDRI
                     DTIEIDES"
     gene            8372..10540
                     /locus_tag="VIN32_03315"
     CDS             8372..10540
                     /locus_tag="VIN32_03315"
                     /inference="COORDINATES: protein motif:HMM:NF013123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HEY8921642.1"
                     /translation="MSPQLLTLDDVARRPEPGMDAPGSAVFAPDGRSITYLHSPDGSL
                     VRSLWRHDLASGKRVEIAVPLPETTREETLSRADHLLRERTGAIELGVTSFAWAGDAQ
                     EPTLLIPMNGRLFVAFGPQIERGVHEIAGADHAGGASLSPDGRFVSFSMGGDLHVAAV
                     HDGPPRRITHDAEEGVFNGLAEFAAAEELGRFEGAWWSADSQFLAFAHVDERGIPPIT
                     IAHATGSEPRDEVHHYPFAGGPNAHVTLRIASIPGSAWGQVFLPMQADDYLARVVADP
                     SGGWLVAVLPRAQRTLEWLRVGVDGSTEALWTEESEPWINLDDDTRVLADGRILRSTE
                     RSGFRHLELRSPAGDLERVLTQGEWAVTSVVAISAARGEVLFTGTRDGVLERHLYAVP
                     VDPAEPTADPERLSTEPGWHTVVAAPDGERWIDTWSDLRHAPRLTVETREGDSVLIHA
                     SSTTAEEAQLDPPELVELVAADGRTTLHAAVYRSAAPRGAPAPAALWVYGGPHWQYVK
                     NDWELTAFGLRQYLAQHGATVVVVDSRGTESRGLAFESAVNRQMGWNEVADQAGALRQ
                     LAARGELDLGRVGVVGASYGGFMSIMAMAREPDLFGTGVAVAPVSEWIGYDTAYTERY
                     LGLPSENADGYRESSALMHVGAVHGDLLLIHGTVDENVHLRHSERLVEAFHGAGRKVE
                     LVRLPEQRHRTRGGAIRVREQRTIAHLLRGLGLPLPEELG"
     gene            complement(10547..10804)
                     /locus_tag="VIN32_03320"
     CDS             complement(10547..10804)
                     /locus_tag="VIN32_03320"
                     /inference="COORDINATES: protein motif:HMM:NF022182.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HEY8921643.1"
                     /translation="MCHCLRKDASILAAETLDCRDRQGVTQAAQDADMTIQLSCPWCT
                     DEVTFTINEADEELVCDSCSTRMDFAPDPGVTYELLYASVA"
     gene            10813..11106
                     /locus_tag="VIN32_03325"
     CDS             10813..11106
                     /locus_tag="VIN32_03325"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEY8921644.1"
                     /translation="MDATLNADLLRDRAAAIARALSDAKRLCVVERLADGERSVSDLS
                     RDVGCQVPNMSQHLSVLRAAGIVASRREGSTVFYRLAHPQVLEVYRMLRQVAG"
     gene            11139..11477
                     /gene="trxA"
                     /locus_tag="VIN32_03330"
     CDS             11139..11477
                     /gene="trxA"
                     /locus_tag="VIN32_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012810252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEY8921645.1"
                     /translation="MSNIREATDANWEQMVIQSDKPVIVDFWAAWCGPCRLVAPEIEK
                     LAEKYAGSVEVLKLDVDANPHTAMHYGVMSIPTVAFFAPGEPPKAAVGFRPAEQLETA
                     FGLEAFAKSA"
     gene            11507..13561
                     /locus_tag="VIN32_03335"
     CDS             11507..13561
                     /locus_tag="VIN32_03335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921646.1"
                     /translation="MRRATSSIVPVAAAAALILLGLGPALLPGRVALAADRGLVVIAQ
                     TRYQALPEERRVHVTIDAVATSYTPNPEDGLAYYPSAIFAVQAGATNVAASSGGQRLA
                     IAIDTSDPDFVGVTVTFPEGVYFEQSYPYRVTFDLPDPGGAPDRNLRISSSIVSFPIW
                     AFGSSGEPGGSVTVILPGGFRPTVQGEDLTPSVGAGGEIVLATTSLPDPFDFFAYLSA
                     DRAGAFSDALLTVQVGGRPAPLKVRAWQDDPEWGTAMIALMTDGLPALQRLIGLPYEA
                     PGTLVVEEAATSRLGDYAGIYNKLSGIIRVRYDADAYVGLHEAAHIWFNGDLFRDRWI
                     GEAYAEFYGVQAAHAIGATGDAFDLTDALMANKIPLNDWGEIGGVEIGVEQYAYAASY
                     HLAGLIFGRTNVAGLQAVWRGADASEMAYQPANGGGDPQIAGDVNLQGWQQLLDLLDQ
                     RAGANFDDLWTDWVVNKDEQRQLQERAVARDQYASVVEEAGPWNLPKDLRGSLGSWRF
                     DEAEAALALAGTVLDARGEVASRASDLHLTPPPELEVLFERDGGLKAAKAEAGLQVAV
                     LTDISVTTERVAEKETVLESIGLLGANPASDLEAARDAYEADDLDLASRDAERALAER
                     TGAAEAGQTRVLVAGGGVVLLTGATVAGVRFRPRRRAVVSAAPAATEPGEPAGERLEP
                     PA"
     gene            13558..14922
                     /locus_tag="VIN32_03340"
     CDS             13558..14922
                     /locus_tag="VIN32_03340"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8921647.1"
                     /translation="MNNGASRSIRAVLVGTFTLRFSTGLTGGLFSYYATQLPAHGGVE
                     MTAFLLGLMMATYFVAELILSPGFGILSDHLGAHRVMQWGPVFGAVAVVLTFATTDLY
                     LLGVTRFIEGAAAGASVPSILGYIAIATTRDVPLRGRTVARFEAATIAGLGIGLVAAG
                     PLWEVFARVAFLLNAGLYGVSFVIYRWGVTDLASQGHPPEGEPVDSAEVRPTRFDFAR
                     YRRVISSPRVWLLAPTWIALNAVIGTWTSQSIFQLVRERSPKFADQLLMGGFRPNEIS
                     IGLAGALLVFFVGLLFWGGRFKAYRRTTIIVIGLAGGLLMVGAAFGLNHSAGFGLLLQ
                     LPLGLVALGGLFVLAGATPAALGLLADISEAHPADRGAIMGLYSVFLALGQITGALLG
                     GGAAQWQGIDGLLAASLGLLLFALLPVRALRASEHLVGLAHDDHGEGPPAATTEAKAD
                     PEPD"
     gene            15186..>16879
                     /locus_tag="VIN32_03345"
     CDS             15186..>16879
                     /locus_tag="VIN32_03345"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEY8921648.1"
                     /translation="MRVSKLFFTSLRDNPADAEMASHRLLVRAGYIRQLGAGIYSLLP
                     LGFRVSQRIEQVIREEMDAIGGQEMEMPVVHPAELWKESGRYAKIGPEMVRFKDRGGR
                     DMVLAMTHEEVVADLLRDIVKSYRQLPAIVYHFQTKFRDEPRARGGLIRVREFVMKDS
                     YTLDADEAGLDRSYQLHYGAYERIFRRLDLETVVVGADVGIMGGSLAHEFMLLNDAGE
                     DTLVICETGDYAANQQIAIVAKPEPPVEEARPTEEVATPETPTIAALAEFLGVGEDRA
                     AKAIFFVTGDGRLVTAIVRGDFEVNETKLSNAVKAIGGLRPAQAEEIVVAGMVPGYAS
                     PIGATGTTVVVDDLVARSPNLVAGANKHGYHLLNVNVGRDFQPDLVVDIANARAGDAC
                     PNCGAPVVLRQGIEVGNIFKLGTDFTEKLGATYLAEDGSRNLIVMGSYGIGLGRAMAC
                     IVEAHHDDKGIVWPESVAPYAAHLVSLSASRDPAVAEAAEGLYQRLTDAGVEVLYDDR
                     DESPGVKLTDAELLGMPRIVTISPRSLAAGGAEVTDRASGERSVRPIAEVEAGLVEA"
BASE COUNT         2596 a         5676 c         5873 g         2734 t
ORIGIN      
        1 gtccacggcg acctgattcg ccgcaacgca gggcgcagtc gccgttatcg ccggctcagt
       61 cagggttacc tcgcatggca ccacgagctg gccgccgctg gcgagcgtgc caagctcgtc
      121 gagggtgatc gtcgccttga ggttcgtgag gccggtcacg acggcgagcg ggaaggtgac
      181 gtcggggatg ggcgtggccg tcggagatgg cgacggcgtc gggattgcgc tcggcgacgg
      241 cacgagactg gggctcggac tcgcgcatcc gacgaacatc gtcaagaccg cgacggccgt
      301 caggcgtcgg accgtcgcca tggggttcac ggcgccatcg tagctaccgg taggcgatca
      361 agtgctcggc gcgacccggc cactcggagc accaggctcc tcgatcttgc gtgggcgtcc
      421 gctgaccatc gccaccacgg cgccaatgat cagggcggcg gccaacagtg agcgcggggt
      481 gagcgtctcg ccacggaatg ccacgccgag cgccaccgct accaccggat tgacgtaggc
      541 ataggttgcg accgtcgtca ccgccacgtg gttcagcagc cagatgtagg cggtgaaggc
      601 caccagcgag ccgaacacga tcaggaaggc caggccgagc agcgacgcgg tcgtcacggc
      661 accaggatcg aagcggccga gctcgccgat cgccacgccg gccgccagca gcaccaggcc
      721 accggcgagt tgctccattc cggtccccag cagctggttt cgcgggaggg ggccccggcg
      781 cgcataaagg gagccggacg cccagctgac cgcggccacc accaggatgc cgatcccgat
      841 cgggtcgaac gagtcaagcc cgctcgacgg gagcagcagg atcgccaccc cgcccagccc
      901 gaccagcagc ccgccgatcg ccagaaggct cggcatgcgg cgggtcagca gggcgtcgaa
      961 gaggctgagc cagatgggcg tggtggcgat gatcacggca gcgatgccgg ttgggatcgt
     1021 ctgctctgcg atcgagacca tcccgttgcc gccgagcagg aggagggcgc cgacgatgaa
     1081 tgcggtccgc cactcgaccg ccctcggccg caccagagcc tcgccggtga ggccgcgtcg
     1141 ccgtcgccat cgggcgcgtg ccacgacgaa ggcgaccagg gtcaggcccg cggtgaggaa
     1201 ccgaatgccg gccgccagca gcggcggcaa gctctcgatc atgaaggcga tggccaggta
     1261 ggtcgagccc cagagcacgt agaccgtacc cagcgccgcg gccaccgcca gcgtcgatgg
     1321 gcggccggtg gcgggagtca tcggtgcatt gtgaagtaac cggccacgac gcgtataacg
     1381 gtgtcacatg ccccgtttgt gaggtcgtgt cgtggatgcg gcccgccgtt cgtcgtcatc
     1441 atgaccggac gcagcgggag gattgcagga ccaatgaagt acatgctcat catcgccggg
     1501 gccgatgacg cctggggcca cctcagcgaa gccgaacagg gcgccctgta cgagaaggtg
     1561 cgtgcctggt ggggcgagca ctccgcctcc ggcgagatcc tggacggtca cgagctccag
     1621 cccggatcga ccgcgaccac cattcgacgc gacccgtcgg gcgaggtgac agtcaccgac
     1681 gggccattcg tcgaaggcaa ggagatggtt gccggctacg gcatcctcga cgtgcccgac
     1741 ctcgatgccg ccatcatgct cgcgtcgtcc tggccggccc cggatacgct cgagatccgg
     1801 ccgatcgtcg agcggtagac cagctccgtc agcgcatgtt gccgttctgg ccgatgctca
     1861 tgcgacctgg ctgcggccaa taggtcaatc cgtgcgggct ggcgccgacc gggatggtgg
     1921 tcaggacctc gccggtctgc gtgtcgatga ccgataccga tcggtggtat cggttcgacg
     1981 cccatagctg gctcccgtcc ggcgacagga ccatctcgtc gggggaaccg cccaccgacc
     2041 aggtcgcgac caccttcagg gacgcgaggt cgatgacgga gatggtcgcg gcggcacgat
     2101 ttgccacaaa catctgcgag gtgtcgcggc tgaattccag gccatgggca ccgtcgccgg
     2161 tcgggatgaa ggtggtcacg gccatggcct ccggatccac gacctggacg ccgtcgagcc
     2221 cctcgttggc gacgaagaag gatcggccat ccggcgccag gcggacgtct cggggcaggc
     2281 tgccgacctc gacgttgccg gtcaatgcca tcgcgctggt gtccaccttc gccagctgcc
     2341 cgctgtactc gcagctaatc atcaggtagc tcccgtccgc ggacaggtcc aggtcgtcgg
     2401 cgcccggcca tggcacctgg acgaacttca ggagctccca gctgcctcgg tcgtagaagg
     2461 tcacgccgtt gtccttgacg agtgagggcg agatgtagtc ggtgaccacc atcgccttct
     2521 ggccatcgac ggtgaagtag accgcatacg ggaagatcga aaggccgatg ctgtcgactg
     2581 cctgcgctgt ccggggatcg accaccgaca ggctgccgcc gttcatgttg ctgacgagca
     2641 gccgcttcag gtcccaatct ggggtgacgt gctccgggta cgcacccgtc gtgagcgtgc
     2701 cgacgaccgc gaaggtggca ggatcgatga cggtgacgtc accggacagc tcgttcggca
     2761 cgtagacacg ttccgggacg ccggcaagcg atggatcgat ctggccgctc gtggtggccg
     2821 cgtagacgcc gccaatcggg gtcgggctcg gttcggcagg gagcgatggt gcgacgctcg
     2881 gcagacctgc cgacgggctg gccagagcgc tgccggaggg cgacgaagaa gggctggggg
     2941 aagtcgtgga gcaggccgcc agcaacaggc cggccgtgat ggccagcacc cccccgcctc
     3001 tcatcggctg gaggagggac tcaacgcatg ttgccgttgt ggcccaggct gatggtgccg
     3061 ggctggggcc agaaggtgag gccgtgcggg ttcccgccgg tcgggatgac cttgatcacc
     3121 tccccagtct ccggattgag caccatgacg gtgccgtgga agcggttgga gacccacagc
     3181 tgggtgccat ccggggagag ggcggccatg tccggcgaac cgccgacctc ccacgtgtcg
     3241 acgaccttgc gcgttgccac gtcgatcacc gagatggagc ccgcaaggcg gttcatgacg
     3301 aagagccggg tgacgtcgcg gctgagggcg aacccatgcg ctcccttggc agttgggatg
     3361 aagtcgacgc gctccatgct cagcgcatcc accacgtcga cgccgttggt gccctggttt
     3421 gcgacgaaga agacgtcgcc ggcgggagcg aggcgcacgt cgagtggcga tccgcccagc
     3481 ttgacggagc cctcgaccgt catcgtctgc gtgttgatct tggccaccac gcccgcgctc
     3541 tcgcagctga cgacgaggaa gcttccgtca ggggagaagt cgaggtggtt gactcccggc
     3601 caggggacct gcacgaactt cagcagcttc caggtctccc gatcgtagaa gtagaggccg
     3661 ttgtcggcga tcatgcccat gccgaggtag tcggccacga cgatcgcctt cgagccatcg
     3721 ggcgtgtagt agaggttgta cgggaacgga acctgctgcg tcccggacgg cttctcggtg
     3781 agcggatcga tcaccgtcag cgaggcgccg ttcatgttgt tcacgtacag cttgctgagg
     3841 tcccagtcag gggtgatgtg ctcgggagcg gcgccgacgt cgaagcggcc gatcacggtg
     3901 agggtctgcg ggtcgatgac cgccacgtcg ttcgacagct cgttcggcac gtagacccgc
     3961 agcgggaggt ccgcgacccg cggatcgagc tggccgccca cggttccgga atagacgccg
     4021 ccgggcggcg tcgcggttgg ggtcggctcg acggtgggag tgggcaatgg tgtcgcattt
     4081 ggggcgtcac tcgggacggc tccgctcgac gctccgggcg tggcgctctg cccggttgcg
     4141 gagcaggcgg cgagcatgat tcccgtgatg agcagcgtgg tgacgcgccg agtgtgcaac
     4201 cgatgggctc ccagactacc gtggatgccg gttcaggcag gcgccgccca tcctaaccgt
     4261 tccatggcat ggggggccaa tcgccagctc gggagtttcc ttgaccgaac gccacgccgt
     4321 cgacagctcc ctggacctgg tcctgcggga ggagcggacg cgactgatcg cctcgctggt
     4381 gcgcatcctg ggtgactggg accttgccga ggagctcgtg caggaggcct ccgtctcggc
     4441 gcttgagcac tggccgatcg acgggctgcc ccgcaacccg ggcgcctggc tgatgacaac
     4501 ggcacgccgc cgggccatcg accggctgcg gcgagacgcg cgatttcggc agcggctgga
     4561 acagctcggc gcagaggcgg aagagatgga gcagccgatc gcaaccggcg ggccgctcgc
     4621 cgcagacgat cggctccgcc tcctcttcac ctgctgccac cccgcgctgt cgcaagacgc
     4681 ccaggtggca ctgacgctcc gcacggtcgg cggcctcgac acgacgcagg tggcgcgcgc
     4741 cttcctcgtc ccggagtcga cggtcgccca gcgcctggta cgggcaaagc gcaagatccg
     4801 tgacgccggg atcccgttcc gcatcccgga ggcgagcgag ctgccaggcc ggctgcaagg
     4861 ggtattggcc gtcctctatc tcgtcttcaa cgaggggtat ctcgcctccg acggcgcggc
     4921 tgcagatcgc cgcgacctgg cccgcgacgc cgaatggctc acttcgctgc tcgtgcggct
     4981 gatgccagat gagccggagc cactcggcct gctggcactc atgcgattgc acctggccag
     5041 gatcgaggca cgcttcgcac ccgacgggtc gctgatcctt ctgccggacc aggaccgctc
     5101 gcgatgggac gggtcagcta tcgccgatgc cggcgacctg gtcgaacgcg cgctgcgcat
     5161 gggtcgcccg gggccgcacc tcctgcaggc ggcgatcgtg gcggtgcacg ccaccgcgcc
     5221 ctcatatgcc gcgaccgact gggcagagat cgtggccctg tacgaccgcc tgttcacgct
     5281 gcagccgacg ccggtggtgg ccctgaatcg ggccctcgcg tcggccgagg tctcgggtcc
     5341 cgagatcgcg ctcctggaga ttgaaccgct ggtgacgcgc ctcgacggat atcacctctt
     5401 ccacgcggcg cgcggcgagc tcctacgcag gctcgggcgg ctggacgaag cccgatcggc
     5461 cgatcgtcgt gcgctgtccc tgaccgacaa cccggctgaa cggcggctcc tcgaggagag
     5521 gctcaggcac cccacggccg atggcgcgta acgaatcggc cggggtgggc gtccccgggg
     5581 cacaatgact cgcatgcagc tctcacgccc cgcgctcggc ggcctgaccc tcatcgtcag
     5641 cctggcactc gccggctgcg ccgccggaag cccctcggtc gcgccgagcg actcgccgat
     5701 cgcctccccc agtgccgcgc cgtccaccac ggcgtcagag gccgcaagcg tcgaggcatc
     5761 ggctgccgcc agcgagccga ccgagagcct gccggcgttc gtctgcactc catcggccac
     5821 catcgcctcg acgaccaacc gggcccagat caccgacgtc agggtcggca cgcatgatgg
     5881 gtacgaccgg gtcgtgttcg agttcgcatc gggcctcccc gacgccgtca tcgaaggggt
     5941 cttgcctccc atgtacgcgg atccaagcgg catcgagatg gacgtcgccg gaaccgcgtt
     6001 cctgcgggtc acgatgcacg gcgccaccaa gctgtccccc gagggtggcc tgacctacgg
     6061 cgggtcgacc agcttcaagc ccggcttcga ccggctggcg cagctgatcg aaggcggcga
     6121 cttcgaggcg gtgagcacct ggtacctcgg cctgaacggc gccggctgct tccgagtcct
     6181 ggccctttct gacccgagcc gcctggtgat cgacatcgag cactgagcgt tgacgcccga
     6241 gaggatcatg gctggaccga tggattctgc tccggccgtg gtccgtgcct cggcggggat
     6301 cggtctcgtc gcgctggcgt cgggactgct cgcccagttc ctcttcgtcg atgcggcgtt
     6361 cgggatcaat gtcctgatcg cgacccttgc tgtgctgctc gctgggtggg tcgtgcgcga
     6421 cgggcccacg ccgcgacccc gcctgcttga tggctggctg gcgccgggag cactgatcct
     6481 cgctgccttt gtctccctgc gcggcgatgg cacgctcgtg gcgctcgacg tcgtgggcgc
     6541 catcgccctt tctggtgcgg ccctcgtcag cttcggcggc ctgcgagttc tggagcgtcc
     6601 attcgccgcg atcgcaggcc tgggcgcacg tttggcgctg tctgggatcg tcgggggcgt
     6661 gggacctttc gcggctctcg cgagaatgac atcgacgctg cggggacgct cacgcccggg
     6721 cagattcgtg ccagtcctgc gcggcctgct gatcgccgtg cctctcgtgc tcgtcttcgt
     6781 cgcactcttc tcgtccgctg acgcggtgtt tgcgcggatc accggagacc tcctcgtctg
     6841 gaatctcgat ctcggatcac tgccgagtcg gctcgccctc gcggcgatcg tggcgtgggt
     6901 cgcggcaggc ctgttcgcgt tcgtggccag aggggaggat cgcccgctcg ggaccacgac
     6961 ggatgcggtc gcatccgatc gaccccgcct cggctccgcc gaggctgtga ccgtgctcgt
     7021 ggtgctggat cttctcttca tcggcttcgt ggtactgcag ggggcgtacc tgttcggagg
     7081 ccgtgacacc ctcgatgcga gcggcatcac ctacgccgag tacgcccggc gtggcttctt
     7141 cgagctgttg gccgtcgcgt tcctggtcgc tggattgatc cttgccctgg agggctttgt
     7201 gcggaccagg acgcggacct acgtgctgac cgccatcggc ctggtgctcc tgacgatcgt
     7261 ggtcctggcc tccgcattcc tgcgcctgcg cctgtaccag gacgcatatg gatggacgga
     7321 gctgcgcttc tacgtgctgg cagccatcgc ctggctagcg atcggcgcgg tgatcgccac
     7381 ggtgaccctc gccaggaacc ggacccgctg gctcatccac gggatgctgg tgctctcgtt
     7441 cgtgttcggc ctgttcttca acgtcatcgg gccggtgaag ttcatcgccg agcagaacat
     7501 ccagcgcgcg atccaccccg agctcgttgc cgcgggagga gagacgggtc tcgacgtcta
     7561 ctacctcgca atcctcgggg acgatgcgct atcggtcatg gccgagaatc tctgcgcgct
     7621 gcctggcccg gtagcgaagc aggctctcga ggcggtgacg atgtggaggc gcttcctggg
     7681 tcgcgatccg gcgggaaagg cctggcaggc gtggaatctg tctcgcgagc gggcgcgaga
     7741 tacgcggctg caaccggtct gcctgctgtc tagccgctga gcgcagggcg ccctcctcgg
     7801 ctagcatcgg tctgtcccat tccgcaccct cggagtaagc atggctgacc gattggcggc
     7861 gcccagcggc acgctcgacc tcaccaagtc gtattcggcg cgcttcaaga cggaacgcgg
     7921 tgagttcacg tctgagctgt tcgccgcgga cgcaccgatg acggtggaga acttcgtgaa
     7981 cctggcccgt gcgggcttct acgacggcac gaccttccac cgcgtgatcc cgggcttcat
     8041 ggcccaggct ggcgatccga ccggcaccgg aaccggcggc ccgggctact ccttccgtga
     8101 cgagctgtcg gttaagcgcc gccacgaggg gcccggcacg ctctcgatgg ccaatgccgg
     8161 cccgaacacg aacgggtcgc agttcttcat cacctttggc gcgacgccgc acctcgatgg
     8221 gcggcacacg gtgttcggcc gcgtgacgtc cgggatggac gtggtgaaca gcatccgcga
     8281 gcgcgacccg cagcgcgact cgcagcccgg agaccggatc gacacgatcg agatagacga
     8341 gagctgacct cgggccgttc tggcatacat cgtgagcccg cagcttctga cgctcgacga
     8401 cgtggcccgt cgtcccgagc ccggcatgga tgcccccggg agtgccgtct tcgcccccga
     8461 cggtcggtcg atcacctacc tgcacagtcc ggatgggtcg ctcgtgcggt cgctgtggcg
     8521 ccacgacctg gcgagcggca agcgggtcga gatcgccgtc ccgctgcctg agacaacccg
     8581 cgaagagaca ctttcgagag cggatcatct gctgcgcgag cgcacgggag cgatcgagct
     8641 gggcgtgacc tcgttcgcgt gggcgggtga cgcgcaagag ccgaccttgc tgatccccat
     8701 gaacggccgc ctcttcgtcg cgttcgggcc acagattgaa cgcggcgtcc acgagatcgc
     8761 gggcgctgac catgctggcg gtgcatccct cagccccgac ggcaggttcg tctcgttctc
     8821 catgggcggt gacctgcacg tggcagcggt gcacgacggg ccaccgcgac gaatcaccca
     8881 tgacgccgag gagggcgtct tcaacggact ggccgagttc gcggcggccg aggaactcgg
     8941 gcgattcgag ggcgcctggt ggagcgccga ctcgcagttc ctggcctttg cgcacgtcga
     9001 cgagcgcggc atcccgccca tcacgatcgc acacgccacg gggtcggagc cgcgcgacga
     9061 ggtgcatcac tatccgttcg ccggtggccc aaatgcgcac gtgaccctgc gcattgcgtc
     9121 gatccccggt tcggcgtggg ggcaggtctt ccttcccatg caggccgacg actacctggc
     9181 gagggtggtc gccgatccat ctggcggctg gctcgtcgcc gtgctgccgc gggcgcagcg
     9241 gacgctggag tggcttcggg tcggggtgga tgggtccacc gaggcgctat ggacggagga
     9301 aagcgaacct tggatcaacc tggacgacga cacgcgggta ctggccgatg gccgcatcct
     9361 gcgttcgacg gagcgcagtg gctttcggca tctggagctg cgctcccctg ccggcgatct
     9421 cgagcgcgtg ctgacgcagg gagaatgggc cgtaacgtca gtggtggcca tcagcgcagc
     9481 gcgaggcgag gttctcttca cggggactcg cgacggagtc ctcgaacgcc atctgtacgc
     9541 tgtaccggtc gaccccgccg agccgaccgc agacccggag cgcctgagca cggaacccgg
     9601 ctggcacacc gtggtggccg ccccggatgg cgagcgctgg atcgacacct ggtcggacct
     9661 ccggcatgcc ccgcgcctca ccgtcgagac ccgcgagggc gactccgtgc tcattcatgc
     9721 gtcgtccacg accgcggagg aagcgcagct cgatccgccg gagctggtcg agctggttgc
     9781 cgcggacggg cggactaccc tgcacgccgc ggtgtaccgc tccgccgctc cgcggggcgc
     9841 ccctgcaccg gcggcgctgt gggtctatgg cggaccccac tggcagtacg tgaagaacga
     9901 ctgggaactg accgcgttcg gccttcggca gtacctggcc caacacgggg cgaccgtggt
     9961 cgtggtcgac agcaggggaa ccgagagccg ggggctggcg ttcgaatcag cggtcaaccg
    10021 tcagatggga tggaacgagg tcgcagatca ggcaggtgcc ctgcggcagc tggcggcgcg
    10081 tggcgagctg gatctgggca gggtcggcgt cgtcggggcg agctacggcg gattcatgtc
    10141 gatcatggcc atggcccgcg agcccgatct cttcggaacg ggggtggcgg tcgccccggt
    10201 aagcgagtgg atcggctacg acaccgcata cacggagcga tacctgggtc tcccgagcga
    10261 gaacgcggat ggctaccggg agtcgtccgc gctcatgcat gtcggggccg tgcacggcga
    10321 cctgctgctg atccacggca ccgtcgacga gaacgtgcac ctgcgacatt ccgagcgtct
    10381 ggtggaggcg ttccacggcg ctggcaggaa ggtcgagctc gtcaggctgc cagagcaacg
    10441 ccaccgcacg cgaggtggcg ccatccgcgt ccgcgagcag cggacgattg cgcacctgct
    10501 gcgcgggttg gggctgccac ttcccgagga gctgggctag gtcggatcag gcgaccgacg
    10561 cgtacagcag ctcgtaggta acgccggggt cgggagcgaa gtccatgcgc gtggagcagc
    10621 tgtcgcacac gagctcctca tcggcctcgt tgatggtgaa ggtgacctca tcggtgcacc
    10681 aggggcagga aagttgaatg gtcatgtcgg catcctgtgc tgcctgcgtg acaccttgcc
    10741 tgtcgcggca atccagcgtc tcggcagcga gaatacttgc gtccttgcgc aagcagtgac
    10801 acaatagtcg ggatggacgc cacgctcaac gccgatcttc tgcgcgaccg cgccgccgcg
    10861 atcgcccgag cactctcgga cgccaagcgg ctgtgcgttg tcgaacggct ggccgacggc
    10921 gagcgatcgg tgagcgatct gtcccgcgac gtgggctgcc aggtgccgaa catgagtcag
    10981 catctctcgg tcctgcgggc agcggggatc gtcgcctcac gacgcgaggg gagcactgtc
    11041 ttctatcggc tcgcgcaccc acaggtgctc gaggtctatc gcatgcttcg acaggtcgcc
    11101 ggctgagcgc gacacaggga aaggaccaag caagacccat gtccaacatc cgcgaggcga
    11161 ccgacgccaa ctgggagcag atggtgatcc agagcgataa gccggtcatc gtcgacttct
    11221 gggccgcctg gtgcggtccc tgtcgactcg ttgcacccga gatcgagaag ttggcggaga
    11281 agtacgccgg ctcggtcgag gtgctgaagc tcgacgtgga cgcgaatccg cacaccgcca
    11341 tgcactacgg cgtgatgagc atccccaccg tcgccttctt tgcacccggc gagccgccga
    11401 aggcagcggt cggcttccgg ccggctgagc agctggagac cgcattcggg ctcgaggcat
    11461 tcgccaagag cgcctgacgg cggtctccgc ctatcctgcg ggggtcatgc gccgagcgac
    11521 aagcagcatc gtccccgttg ccgcggccgc ggcactgatc ctgctgggcc ttggcccggc
    11581 gctgctcccc ggccgcgtcg cgctggccgc ggatcgcggt ctggtcgtga tcgcgcagac
    11641 ccgctaccag gcgctccccg aggagcgccg ggtccacgtc acgatcgacg cggtggcgac
    11701 ctcctacacc ccgaatcccg aagacgggct cgcctactac cccagcgcca tcttcgccgt
    11761 gcaggcgggc gccacgaacg tggccgcgtc atcgggcggg cagcggctgg cgatcgccat
    11821 cgacacctcg gaccccgact tcgtgggcgt gacggtcacc tttcccgagg gggtctactt
    11881 cgagcagagc tatccgtatc gggtgacgtt cgacctgcct gacccgggcg gtgcgccgga
    11941 tcgcaacctg cggatcagct cgagcatcgt ctccttcccg atctgggcct tcggaagctc
    12001 tggcgagcct ggcggaagcg tgaccgtgat cctgcccggc gggttccgcc caacggtgca
    12061 gggtgaggac ctgacccctt cggtgggggc cggaggcgag atcgtcctgg ctacgacctc
    12121 tctccccgat ccgttcgact tcttcgccta tctgtccgcg gatcgcgcgg gcgcgttcag
    12181 cgacgcgctg ctcacggtcc aggttggtgg tcggcccgct cccctgaagg tgcgcgcctg
    12241 gcaggacgac cccgagtggg gtacggccat gatcgccctc atgaccgatg gattaccggc
    12301 gctgcagcgc ctcatcggcc ttccctatga ggctcccggg acgcttgtcg tcgaggaggc
    12361 cgccacgagc cgcctgggtg actatgccgg catctacaac aagctgagcg gcatcatccg
    12421 ggtccgctac gacgccgatg catacgtcgg gctgcacgag gccgcgcaca tctggttcaa
    12481 cggagatctc ttccgcgacc gatggatcgg cgaggcgtac gcggagttct acggggtgca
    12541 ggcggcgcat gccatcggcg ccaccggcga tgccttcgac ctgaccgatg cactcatggc
    12601 aaacaagatc cccctcaacg actggggcga gatcggcggc gtcgagatcg gggtggagca
    12661 gtacgcctat gccgccagct accacctcgc cgggctgatc ttcgggcgaa ccaacgtggc
    12721 cgggctgcag gccgtgtggc gcggggccga tgccagcgag atggcgtacc agcccgcgaa
    12781 cgggggcggc gatccgcaga tcgccggcga tgtcaatctc cagggctggc agcagctcct
    12841 cgacctgctc gaccagcggg ccggcgccaa cttcgacgac ctctggaccg actgggtggt
    12901 caacaaggac gagcagcggc agcttcagga gcgcgccgtc gcacgcgatc aatacgcgtc
    12961 ggtggtcgag gaggcgggcc catggaacct gccgaaggac ctgcgaggct ccttgggatc
    13021 gtggaggttc gacgaggccg aggcagccct cgcactggcc ggcacggtcc tcgatgcacg
    13081 cggcgaggtg gcgtccaggg cgagcgacct gcatctcact cccccgcccg agctcgaagt
    13141 actcttcgag cgagacggtg gcctcaaggc cgccaaggcc gaagccggcc tccaggtcgc
    13201 ggtgctcacg gacatctccg tcaccaccga acgggtcgcg gagaaggaga ccgtcctgga
    13261 gtcgatcggc ctgttggggg ccaaccccgc gtccgacctc gaggctgcgc gcgacgcgta
    13321 cgaggccgat gacctggacc tcgccagccg ggatgcggag cgggcgctgg ccgagcgcac
    13381 cggggccgcg gaggccggcc agactcgtgt gctggtcgcg ggaggcgggg tcgtgctgct
    13441 gaccggcgcg acggtcgccg gcgttcggtt caggccgcgg cgtcgagccg ttgtgagcgc
    13501 tgcaccggct gcgaccgaac ctggtgagcc ggccggcgag cgcctggagc caccggcatg
    13561 aacaacggcg ctagtcgttc gatccgcgcc gtcctcgttg gcacattcac gctgcgattc
    13621 agcacgggcc tgaccggggg gctcttctcg tactacgcca cccagctccc ggcgcatggc
    13681 ggcgtcgaga tgaccgcctt cctgctcggc ttgatgatgg ccacctactt cgtcgccgag
    13741 ctgatcctgt cgccagggtt cggcatcctc tccgaccacc ttggggcgca ccgggtgatg
    13801 caatggggcc ccgtcttcgg ggctgtcgcg gtggtcctca ccttcgccac caccgatctc
    13861 tacctgctcg gcgtcacccg cttcatcgag ggagccgccg ccggcgccag cgtcccatcg
    13921 atcctgggct acatcgccat cgccaccacc cgcgacgtgc cgctccgcgg ccggacggtc
    13981 gcgcgcttcg aggcggccac cattgccggc ctggggatcg ggctggtcgc ggcaggcccc
    14041 ctgtgggagg tcttcgcgcg cgtcgccttc ctgctcaacg cgggcctgta cggcgtctcc
    14101 ttcgtcatct accgatgggg cgtcacagac ctggcgagcc agggccatcc gccggagggc
    14161 gagccggtcg attccgccga ggtgcgtccg accaggttcg atttcgcgcg ctaccggcgg
    14221 gtgatcagca gcccgcgagt gtggctcctg gcaccgacct ggatcgccct caacgccgtc
    14281 atcgggacat ggacctcgca gtcgatcttc cagctggttc gcgagcgttc gccgaagttc
    14341 gccgatcagc tcttgatggg aggcttccga ccgaacgaga tcagcatcgg cctggcgggg
    14401 gcgctcctcg tcttcttcgt cgggctgctg ttctggggtg gccgattcaa ggcctatcgg
    14461 cgcaccacca tcatcgtgat cggtctcgcg ggcgggttgc tgatggtggg tgctgcgttc
    14521 ggcctcaacc actcggccgg cttcgggctc ctcctgcagc tgccccttgg cctcgtcgca
    14581 ctgggtggcc tcttcgtgct ggctggtgcc acgccggccg cgctcgggct gctggccgat
    14641 atcagcgagg cgcatccggc cgaccgcggc gcgatcatgg gcctgtactc ggtcttcctg
    14701 gcccttggcc agatcactgg cgccctgctg ggagggggtg ccgcccaatg gcagggcatc
    14761 gacggcctgc tggccgcctc gcttggcctc ctgctgttcg ccctcctgcc ggtgcgcgcc
    14821 ctgcgcgcca gcgagcacct ggtcggactg gcgcacgacg accatggcga aggcccgccc
    14881 gcggccacca ccgaagccaa ggccgatccc gagcctgact gagccgatcc tggccgtcga
    14941 atcgcagctg gcttcctact gccggtcaaa gttgccagat cgcaatgggg ttggcgttag
    15001 gcggcgctcg aaggcagcgg gtgaggcccc gcaggccaag ttgagcgtgg gtcggtcgcc
    15061 ggatcggcat gcgaaggcgg tcgaatctgc ctaggagcag ccaggctgcg atctggcgac
    15121 gaatcggcgc gataaggccg ctcacagccg ccggcgaggg accggtccgc tattcttcgc
    15181 cctccatgcg cgtctccaag ctcttcttca cctcgctccg cgacaacccc gccgacgccg
    15241 agatggcctc gcatcggctg ctggtgcgcg ccggatacat tcgccagctg ggagcgggga
    15301 tctattccct gctgccgctg ggcttccggg tcagccagcg catcgagcag gtgatccgcg
    15361 aggagatgga cgccattggc gggcaggaga tggagatgcc ggtcgtccat cccgccgagc
    15421 tgtggaagga gagcgggcgc tacgccaaga tcggccccga gatggtccgc ttcaaggacc
    15481 gtggcgggcg cgacatggtg ctggccatga cgcacgagga ggtcgtggcc gacctgttgc
    15541 gggacatcgt caagagctat cggcagctgc ctgccatcgt ctaccacttc cagaccaagt
    15601 tccgcgacga gccgcgcgcc cgcggcgggc tgatccgggt gcgcgagttc gtgatgaagg
    15661 actcgtacac cctggatgcc gatgaggccg ggctggaccg ctcctaccag ctccactacg
    15721 gcgcctatga gcgcatcttc cggcggctgg acctcgagac ggtggtggtg ggagccgatg
    15781 tcgggatcat gggcggcagc ctcgcgcacg agttcatgtt gctcaacgat gccggcgagg
    15841 acaccctggt gatctgcgag accggcgact acgccgccaa ccagcagatc gcaatcgtcg
    15901 ccaagcccga gccaccggtc gaggaggctc gcccgaccga ggaggtggcg acccccgaaa
    15961 cgccgaccat cgccgccctg gccgagttcc tgggggtcgg cgaggaccgc gcggccaagg
    16021 cgatcttctt cgtcaccggc gacggtcggt tggtgaccgc catcgtgcgc ggcgacttcg
    16081 aagtcaacga gacgaagctg agcaacgcgg tgaaggccat cggcgggctg cgccccgcgc
    16141 aggcagagga gatcgtcgtc gcgggaatgg tcccgggtta cgcgtcaccc atcggcgcca
    16201 ccggcacgac ggtggtggtg gacgacctgg tcgcgcgctc gcccaacctg gtggcgggcg
    16261 cgaacaagca cggctaccac ctgttgaacg tgaatgtcgg tcgcgacttc cagccggacc
    16321 tggtggtgga catcgccaac gctcgcgccg gcgacgcctg cccgaactgc ggcgctccgg
    16381 ttgtgctgcg ccaggggatc gaggtcggca acatcttcaa gctggggacc gacttcaccg
    16441 agaagctggg cgccacctac ctcgccgagg atggcagccg caatctgatc gtcatgggca
    16501 gctatgggat cgggctcgga cgggcgatgg cctgcatcgt cgaggcgcat cacgacgaca
    16561 aggggatcgt ctggccggag tccgtggcgc cctacgccgc ccacctggtc tcgctgagcg
    16621 catcccgcga tccggccgtg gccgaagcgg cggaaggcct ttaccagcga ctgaccgatg
    16681 ccggcgtcga ggtgctctac gacgatcgtg acgagtcacc cggcgtgaag ctgaccgacg
    16741 ccgagctact cggcatgcca cgcatcgtca ccatctcgcc cagatccctg gcagcgggcg
    16801 gcgcggaggt gaccgatcgg gccagcggcg agcgctccgt gcggcccatc gccgaggtag
    16861 aggcggggct cgtggaggc
//
LOCUS       DATCQR010000099         2195 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_114344, whole genome shotgun sequence.
ACCESSION   DATCQR010000099 DATCQR010000000
VERSION     DATCQR010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2195
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114344"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1010)
                     /locus_tag="VIN32_03350"
     CDS             complement(<1..1010)
                     /locus_tag="VIN32_03350"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY8921649.1"
                     /translation="MTRGVVVVGGGFGALEIALALRKRDLNLPVRVITSETEIVYRPW
                     LIRVPAGGLQPPVIPFARLLAAAGADVVAGRVTAVDLEQRHVLLDTGAHIEYDQLIVA
                     TGAVADRGRVPGGREHAVFPCDLVDAAEFAARVSAASAHVAVVFGWERPGPGLEYAAW
                     IAARRPGVRVTAIDGDGTMERRFGAKATARVRALFEQRGAQLVTQGTVERIGEGTVEL
                     GGSSVAADLIAVVAPLRGSTEWLPESLLDERGMLRVDSAMAAGARIFGIGDVVAVPDG
                     YRLPPALRSIQATAPGIARNVAGALGGAAPQAVLRPGQPDTMLPDLAGTAVLVRDRRL
                     LLG"
     gene            complement(1007..1207)
                     /locus_tag="VIN32_03355"
     CDS             complement(1007..1207)
                     /locus_tag="VIN32_03355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921650.1"
                     /translation="MNGLSVLASGLLAGVLVFGGLWILIAGHQATLAGARRRSGKSQR
                     RAADDASDSTTDIRHAAAGVGA"
BASE COUNT          352 a          771 c          728 g          344 t
ORIGIN      
        1 ccgaggagca accgccggtc tcgaacgagc acggcggttc ccgccaggtc cggcagcatc
       61 gtatcgggct ggcctgggcg gagaacggct tgcggggcgg cgccgccgag cgcgccggcc
      121 acattccggg cgatccccgg agccgtcgcc tggatcgagc gcagcgccgg tgggaggcgg
      181 taaccgtcgg gcaccgccac cacgtcgccg atcccaaata tgcgggctcc agcggccatc
      241 gcactgtcga cgcgcagcat gccccgctcg tccagaagac tttctggcag ccactcggtg
      301 ctgccgcgaa ggggcgcgac gacggcaatc aggtcggccg cgaccgagct cccaccgagt
      361 tcaacggttc cctcgccgat ccgctcaact gtgccttggg tgaccagctg cgctccccgc
      421 tgttcgaaga gggccctgac ccgagccgtg gccttggcgc cgaagcggcg ctccatggtg
      481 ccatcgccgt cgatggccgt gactcgcacg ccaggacggc gggcggcgat ccaggcggcg
      541 tattccagcc ctggtccggg ccgctcccag ccgaacacga ccgccacgtg agcggacgct
      601 gctgagaccc gcgccgcgaa ctcggcggca tcgacgaggt cgcagggaaa gacggcgtgt
      661 tcacgcccac cagggacgcg tccacggtcg gcgacggccc ccgtcgccac gatcagctgg
      721 tcatattcga tgtgggcacc agtgtcgagc agcacgtggc gttgctcgag atccacggcc
      781 gtgacgcggc ctgctacgac gtcggcgcct gccgccgcga ggaggcgggc gaacggaatg
      841 acgggcggtt ggagaccgcc ggctggaacg cggatcagcc acggccggta gacgatctcg
      901 gtctcgctgg tgatgaccct gacgggaagg ttgaggtccc gtttcctcaa agccagggcg
      961 atctcaaggg caccgaatcc gccgccgaca acgacgacgc cgcgtgtcat gcgccgacgc
     1021 cagccgccgc gtgtcgaatg tccgtggtcg agtcgctggc atcgtctgcc gcacgccgtt
     1081 ggctcttgcc gcttcgacga cgggcccccg cgagcgtcgc ctggtggccc gcaatcagga
     1141 tccacaatcc cccgaacacg agcacgccag cgaggaggcc tgaggccagg accgacagac
     1201 cgttcactgc tccacggtac cgcggtttac ccgcgcgggt cacgctcgcc ggacgttacg
     1261 tttcttgggg accctcgccc gcggcgaccc tctagttgcg gaggtgtgcc aaccgaagtt
     1321 tcacgccggc ggtccggcga gcagcaggcc gccctgacac tacgcattcc atcgtgtgtc
     1381 gcggagactc cgaaggggaa cgcgtgaggt acaagaactc gatcaggcag cccgccaaag
     1441 tgggcggtgg ttgagcatcg gccggcatga cagtatcgcc gccatgcttg cttcactcct
     1501 ctatctggtc cttcgtcggc tcctcaccct tgtcgcaccg aaccgtcgct ccgaccatga
     1561 cgcacagatt gagatcctcg tccttcgcca tcagctcgag gtcttacggc ggcaggtcaa
     1621 gcgcggtcta cgggcgccgg gaccgcgcgc tgctcgctgc ggcaagcagg atgcttccca
     1681 gagaacgctg gggcgcgttc ctcgtgcgtc ccgagacgct gctgcgctgg caccggtcgc
     1741 tcgtcgcccg aaagtggacg aggccgcatc gcccgcccgg gcgccccgcg atcgatccgg
     1801 aggtctgcaa gctgatcctt cagatggcga gggagaaccc ccggtggggc tacatgagga
     1861 tcaaaggcga gctgggaaag ctcgggatcc gcgcgtccgc cacgagcatc gcgctgcttc
     1921 ttcgccgctc cggcgtcggt ccggcccctc gccgaggccc gacctggcgc cagttcctga
     1981 aggctcaggc agcgggcatc ctggcgtgcg atttcttcac cgtcgagacg gcgtttctgg
     2041 aagacgctct atgtcctgtt cttcatcgag atcggggcac ggcgcgtgca cgtcacggtc
     2101 tccacgacca accccgactg ctccttcgtc acccagcagg cgaggaacct cgtgatgagc
     2161 ctcgccgagg atcgcacgca gatcggcttt ctcat
//
LOCUS       DATCQR010000100         9276 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_68055, whole genome shotgun sequence.
ACCESSION   DATCQR010000100 DATCQR010000000
VERSION     DATCQR010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 9276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9276
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68055"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..924)
                     /locus_tag="VIN32_03360"
     CDS             complement(<1..924)
                     /locus_tag="VIN32_03360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921651.1"
                     /translation="MSEAERKRLDHVVLPRRLPGLALLERYFPSPADNQLDAELAAFG
                     RPGDTVLDPWAGTGWTARRAIAHGMRVVAADPSPFAQLAAQAFLLAPEPLALDAAFAQ
                     LAASRRVDVPLRQHLEELYATRCSTCRRPVVGEQFIWPRDGDAPGRKVYRCPNCDVSM
                     GGPQERVAPIDDADLAKLGIDRPIEPEPVLPIGDPAADLEDDLPPAPVGLTVEPEPED
                     DPAAPIGEAGGPPSLAPATPDPPTGSLRAGRPRYASTVHPDPAAPPASDVRQSLHYQQ
                     LRDRFPVLDGRGELVDELLDLYTPRNLYALQT"
     gene            complement(933..1769)
                     /locus_tag="VIN32_03365"
     CDS             complement(933..1769)
                     /locus_tag="VIN32_03365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY8921652.1"
                     /translation="MTLDETETINRQRRHLTDIAIGQATRGEWQAAVSTNQQVLELGA
                     DTDAYNRLGKALAELGRHAEALEAYQGALARDATNRIAERNVGRLQVIAGGGAAPAAA
                     GDGKQEKASASDFIEEMGKTGHARLINLLPAKQLASLSAGDAVELVLDGALLVARVGE
                     VEVGQVEPRIGSRLAKLMKAGNRYEAAITTVDRDEVRVIIHEVFAHPANFGKVSFPGS
                     ATGRPGDVRPYIKGTPLRYDDDEESGEESEEETEEVEELDTSLPEFSAEPDVEEELLE
                     EP"
     gene            complement(1890..2108)
                     /locus_tag="VIN32_03370"
     CDS             complement(1890..2108)
                     /locus_tag="VIN32_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921653.1"
                     /translation="MTDAAAARSPDQCLRCGAPIQSMGIEDFRVGGNSGGWKLLFGEW
                     AELGEGTLKLEVFACGSCGKVELRVPHE"
     gene            complement(2105..2881)
                     /locus_tag="VIN32_03375"
     CDS             complement(2105..2881)
                     /locus_tag="VIN32_03375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921654.1"
                     /translation="MTATDAPRHGLWIVGRRIERPETIALALVLAGMVVYGWFAPPFW
                     LAVAIAGQLAIGGMGAVWVIGPVRSRFGFARYATFPMAAVALTLLGQMLSPTAQVAGA
                     PVAALLLFGALWSEMRLPDGRSPRLSIDLMLVGIVFAAAAGIAATFPAASWPPAVLLV
                     VAVATVPALRAAELRGRFGVEAVGQAALHLLAIAQLGASLALLHLPGVVGSAVLALGF
                     HAWAGAAEALDNGARTRAVAIEFGSLALLGLLVALLLRPA"
     gene            complement(2878..4629)
                     /gene="polX"
                     /locus_tag="VIN32_03380"
     CDS             complement(2878..4629)
                     /gene="polX"
                     /locus_tag="VIN32_03380"
                     /inference="COORDINATES: protein motif:HMM:NF006375.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HEY8921655.1"
                     /translation="MPRPRAISRTDSTLISNADMARVFNEIADMLEISGELVYKAVAY
                     RRVADAVERWPDDVAHLFQADEPPKIPGAGPALTTKLAELATTGQLAYYDRMRAQVPS
                     GLLEILKIPGVGPRTVKQLHDELGIDSVDALRAAAEGGTLRGLKGLSARTEENILAAM
                     ARLASRGTRLLLHDADALMSGLVEQLREVRGVRAIEAAGSLRRRKATIGDLDLLAATD
                     DPAQLIKTLDSLTSVEHVLSAGTDKSSIVLTEGPQVDLMVCPPAAWGSHLVHFTGSKE
                     HNVALRGMALDRGLSLSEKGFKVLESGELQPIATEEEVYERLGLPWIAPELREDSGEI
                     QAALAGSLPTLVSLADLNGDTHVHSDWTDGVDSIEVMARAARDLGRAWIVLTDHSPSL
                     GITRGLSVERVAEQREEIGRLNTELAPFRILHGTEMEIRADATLDYPDDVLAGFDVVV
                     ASVHTARGQSTEQLTRRTLAAIDNPNVDVIAHPTGRIVNRRDPIALDWPRVFEAAART
                     GTALELNGSPRLDLDDALARAAGKAGVRLTLASDAHRTEELEQLRYAVSVARRAWLEP
                     GQVIGTRTADQLLELVE"
     gene            complement(4703..4867)
                     /locus_tag="VIN32_03385"
     CDS             complement(4703..4867)
                     /locus_tag="VIN32_03385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921656.1"
                     /translation="MSRGATYLGLLANLRQNEMLYEARRRTHSTEPIRERDGRGFGAS
                     LLRALRITKR"
     gene            5143..5787
                     /locus_tag="VIN32_03390"
     CDS             5143..5787
                     /locus_tag="VIN32_03390"
                     /inference="COORDINATES: protein motif:HMM:NF018984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HEY8921657.1"
                     /translation="MVLDRTTLPEPDQAEELFISFGNTLEFEHGQPVDAVPDIASLLA
                     WLRERRLISGRGLAVEAAHLRRDLDETARRVDRFHHLRNLLHEVAEEVTEAGHPTTEQ
                     LRDLNHILRHGLHYHQLERDPDGTRYTFAQVGDRLDQARATIASSLAQFLADDAPSRL
                     RVCANAGCRYLFVDRSPTGRRRWCDMRTCGNQAKVARHRARARDQLHETSASVS"
     gene            6023..7291
                     /locus_tag="VIN32_03395"
     CDS             6023..7291
                     /locus_tag="VIN32_03395"
                     /inference="COORDINATES: protein motif:HMM:NF015413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEY8921658.1"
                     /translation="MLFLSRLVGRPVRDRHGESLGKVRDLIVALGERYPPVTGLVVRV
                     TAGRDIFLPWTDVEEIDADGARLRTTLIDMESFRQRPDEIRLWLDLLDKQIVDVEGRK
                     IVRVNDIQLAPVDGRLRLVAVDVGLAGILRRLGLAGPGERLARALHLEAENYIEWEDV
                     DPVESSVSSLKLRVPHEALGTLHPADVAHIVEQLAPRDRAGILAALDDERAADVLEEL
                     AADDQVDILEALEPEKAADILEEMGPDEAADLVADLSEERQEQLLGLMEPEEASDVRE
                     LLAYGEETAGGLMTTDFITVTPDMNAEQVIERLRDLHPDADQVYYLYVTDADGVLVGT
                     ITLRGLIVAVPETAVRDFMRSDPISVSVDLDAEEVGAAIARYNLLALPVVDAQGRMQG
                     IVTVDDALERALGEGWRKRLPEIYEPAEDR"
     gene            7299..8615
                     /locus_tag="VIN32_03400"
     CDS             7299..8615
                     /locus_tag="VIN32_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEY8921659.1"
                     /translation="MTRRLALPRPIRRAQRSLRERLPNFRVHRSGLLAFLGVMGPGLI
                     AGLAGNDAGGIATYSVLGAETGLSLLWIFPITTILLVVVLEMAARMGAVSGQGLGDLI
                     RDDFGIRWTLFAMIVLLIANGANIVAEFAGAAAALEIFGISRYATVPIVAAGIWALVV
                     FASYRAVERVFLSVAVVFGAYVVSAFVAGPDWGEVGRALVSPSVSLAPHALLLMVATV
                     GTTVTPYMFFYLQSSVADKGLGPEELQFERADAVLGGVWTNAIALFIVIVTAVTIFPR
                     GITIESADQAALALAPVAGDFAKGLFAFGLFGASVLAATVMPLTTSYAVCESFGWESG
                     VSRKFREAPAFMGLYTALIVIGAVVVLIPGLPLIGVILISQNLNGILLPIILVFMLRL
                     VNNPRIMGEHTNPRWLNVLAWGLTGLVILLTGVLFGSTLASWLGGG"
     gene            complement(8637..>9276)
                     /locus_tag="VIN32_03405"
     CDS             complement(8637..>9276)
                     /locus_tag="VIN32_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010468335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY8921660.1"
                     /translation="MEGRRDQFVSEVMAAEGMGRQRATRLTDAAIDRWVWYAGWADKF
                     SQVNGSVNPVNGSYFNFSVPEPTGVVGVIAPEASSLLGLVSRLAPVIVSGNCAVVLAS
                     ESRPLPAVTLTEVLATSDVPGGVVNLITGLRRELVLHLAGHMDVNALDAYGLDPAQGI
                     AVEELATENVKRLVRPPTNGADAVDWLDPRTQSPYLIGEFVETKTVWHPIGA"
BASE COUNT         1374 a         3206 c         3250 g         1446 t
ORIGIN      
        1 cgtctggagc gcgtacaggt tgcgcggcgt gtacaggtcg agcagctcgt cgaccagctc
       61 gccgcgccca tccaggacgg ggaaccggtc gcgcagctgc tggtaatgca ggctctgccg
      121 gacgtcgctg gcggggggtg cggcggggtc cgggtgcacg gtcgaggcgt agcgcgggcg
      181 tccggcacgg agcgacccgg tcggagggtc gggtgtggcg ggtgcgagcg acggaggccc
      241 accggcctcg ccgatcggcg ccgccgggtc atcctccggc tcgggctcga ccgtcaggcc
      301 gaccggggcc gggggcaggt catcctccag atcggccgcc gggtcgccga ttggcagcac
      361 cggctcaggc tcgatcgggc ggtcgatccc cagcttggcg aggtcggcat catcgatcgg
      421 cgcgacccgc tcctgcgggc cgcccatcga gacgtcgcag ttggggcagc gatagacctt
      481 tctgcctgga gcgtctccgt cgcgaggcca gatgaactgc tccccgacca cggggcgacg
      541 gcaggtggag cagcgcgtcg cgtagagctc ttcgaggtgc tgtcgcaatg gcacgtccac
      601 ccgccgcgat gccgcgagct gggcgaaggc agcgtcgagg gcgagcggct cgggggccag
      661 caggaacgcc tgggccgcca gctgagcgaa tggcgaggga tcggcggcga ccacccgcat
      721 gccgtgggcg atcgctcgac gggcggtcca gccggtgccg gcccatggat cgaggaccgt
      781 gtcgcccggc cggccaaacg cggccagctc ggcgtcaagc tgattgtcgg cgggcgaggg
      841 aaagtagcgc tccagaagtg cgagcccggg gaggcggcgt ggaagaacga cgtgatccag
      901 tcgcttccgt tcggcctcgc tcaatcgggc tcctacggtt cttcgagcag ctcttcctcg
      961 acgtcgggtt cggcggagaa ttccggaagg ctggtgtcca gctcctcgac ctcctccgtc
     1021 tcttcctccg actcctcgcc ggactcctcg tcgtcatcgt agcggagcgg cgtgcccttg
     1081 atgtagggtc ggacgtcacc cggccgcccg gtggcactgc cggggaagct gaccttgccg
     1141 aagttggccg ggtgcgcgaa gacctcgtgg atgatcaccc gcacctcgtc ccgatcgacg
     1201 gtggtgatgg ccgcctcgta gcggttgccg gccttcatca gcttggcgag ccgtgagccg
     1261 atgcgcggct cgacctggcc cacctccacc tccccgacgc gcgccaccag cagggcaccg
     1321 tccagcacca gctcgaccgc gtcaccggcc gagagcgagg ccagctgctt ggcggggagc
     1381 aggttgatga ggcgcgcatg accggtcttg cccatctcct cgatgaaatc cgaggccgat
     1441 gccttctcct gcttcccgtc gcccgcggcc gcgggagcgg cgccaccgcc ggcgatgacc
     1501 tgcaggcgcc cgacgttgcg ctcggcgatc cggttggtgg catcgcgcgc cagcgctccc
     1561 tggtaggcct ccagcgcctc cgcgtgacgg cccagctcgg ccagcgcctt gccgagccgg
     1621 ttgtacgcat ccgtgtcggc gccaagctcc aggacctgct ggttggtgga gaccgcggcc
     1681 tgccattccc cccgcgtcgc ctgcccgatg gcgatatcgg tgaggtgcct gcgctggcgg
     1741 ttgatggtct ccgtctcgtc gagcgtcatc gtctccccaa gctcaatttg aggcggtcaa
     1801 tggtgtggaa atgcccgtcc ggcggggctt cccggccgaa gggcgccttg ttgtagcaga
     1861 gcccggaagg ggtgtcaacg cgcccggcgc tactcgtgcg ggacgcgcaa ctccaccttg
     1921 ccgcaggagc cgcaggcgaa gacttcgagc ttgagcgtcc cctcgcccag ctccgcccac
     1981 tcgccgaaca ggagcttcca cccgccgctg ttgccgccca cccgaaagtc ctcgatcccc
     2041 atcgactgga tcggcgctcc gcatctcagg cattggtcag gcgagcgagc tgcggctgcg
     2101 tcggtcatgc cggcctcagg aggagcgcca cgagcagccc cagcagcgcg aggctgccga
     2161 actcgattgc gacggcacgg gtgcgggcac cgttgtcgag cgcctcggcc gcgccggccc
     2221 aggcatgaaa gcccaaggcg agcacggccg agccgaccac cccggggaga tggagcaggg
     2281 ccaggctggc accgagctga gcgatcgcca gcaggtgcag cgcggcctgg ccgacggcct
     2341 cgactccaaa gcgcccgcga agctcggcgg cacgaagggc cggcacggtc gccaccgcca
     2401 ccaccagcag gacggcggga ggccaggatg cggccgggaa ggtggccgcg atgccggcgg
     2461 cggctgcgaa gacgatcccc accagcatga ggtcgatgct gaggcgcggt gaccgtccgt
     2521 caggcagccg catctccgac cacagcgccc cgaacaggag cagggcggcc accggggcgc
     2581 cagcgacctg ggccgtgggg ctgagcatct ggccaagcag ggtgagggcc acggccgcca
     2641 tcgggaaggt cgcgtacctg gcgaacccga accgcgaacg gaccggacca atcacccaga
     2701 cggcgcccat ccccccgatc gcgagttggc cggcgatcgc caccgcgagc cagaagggtg
     2761 gcgcgaacca gccgtagacc accatgcccg ccagcaccag ggccagcgcg atcgtctcgg
     2821 gccgttcgat gcgccggccg acgatccaca gcccgtggcg cggcgcgtcg gtggcggtca
     2881 ttcgacgagc tccagcagct gatcggccgt gcgcgtcccg atcacctggc cgggttccag
     2941 ccacgcacgc cgggccaccg acacggcgta gcggagctgc tcgagctcct cggtgcgatg
     3001 tgcgtcgctg gccagcgtca gccgcacccc cgccttcccc gccgcgcgag ccagggcgtc
     3061 gtccaggtcg aggcgggggc tgccattcag ctccagcgcc gtgcccgtgc gtgccgcagc
     3121 ctcgaagacg cgtggccaat cgagggcgat cgggtcgcgg cggttcacga tccgccccgt
     3181 ggggtgcgcg atgacatcga cgttcgggtt gtcgatcgcc gccagcgtgc gccgtgtcag
     3241 ctgctcggtc gattggccgc gggccgtgtg cacgctcgct acgacgacgt caaacccggc
     3301 cagcacgtca tcgggatagt ccagggtggc gtcggcacgg atctccatct cggtgccgtg
     3361 caggatacgg aagggcgcaa gctcggtatt cagccggccg atctcctccc gctgctcagc
     3421 gacccgctca acggacaggc cacgggtgat gccgagcgac ggcgagtggt cggtgagcac
     3481 gatccaggcg cggcccaggt cccgtgccgc gcgagccatc acctcgatcg aatcgacgcc
     3541 atcggtccag tccgagtgca cgtgcgtgtc gccgttcagg tcggccaggc tgacgagggt
     3601 cggcaggctg ccggccagcg ctgcctggat ctcgccgctg tcctcgcgca gctcgggcgc
     3661 gatccacggc aggccgaggc gctcgtagac ctcctcctcg gtggcgatcg gctgcagctc
     3721 gccgctctcc agcaccttga atcccttctc tgagagggaa aggccgcggt cgagcgccat
     3781 gccgcgcagg gcgacgttgt gctccttgct gcccgtgaaa tggacgaggt ggctgcccca
     3841 ggcggccggc gggcagacca tcaggtcaac ctggggcccc tcggtcagca cgatgctcga
     3901 cttgtcggtc cccgcgctga ggacgtgctc cacgctcgtc aggctgtcga gcgtcttgat
     3961 cagctgcgcg gggtcgtcgg tagcggccag caggtcgaga tccccgatcg tcgccttgcg
     4021 acgccgcagc gacccggccg cctcgatcgc ccgaacgccg cgcacctcgc gcagctgttc
     4081 gaccaggccg ctcatcagcg catcggcgtc gtgcagcagg agacgcgtgc cacgcgacgc
     4141 gaggcgagcc atggcagcga ggatgttctc ctcggtccgc gccgagagcc ccttcaggcc
     4201 gcgcagcgtc ccgccctccg ccgccgcccg caacgcgtcc accgagtcga tccccagctc
     4261 gtcgtgaagc tgcttcactg ttcgcggccc gacgccgggg atcttcagga tctccagcag
     4321 gccgctcggg acctgggcgc gcatgcggtc gtagtaggcc agctgcccgg tggtcgccag
     4381 ctccgccagc ttggtggtca gcgccgggcc ggcgcccggg atcttgggcg gctcatcggc
     4441 ctggaagagg tgggcgacgt catcgggcca gcgctcgacc gcatcggcga cccgtcggta
     4501 ggcgaccgcc ttgtagacca gctcgcccga gatctcgagc atgtcggcga tctcgttgaa
     4561 gacgcgtgcc atgtcggcgt tgctgatgag cgtcgaatcg gtgcgtgaga tcgcgcgggg
     4621 ccgaggcatc cggccattct aggggcacga aaaaagcggc ccagtcccgg atgaggggct
     4681 gggccgccta ggttggaagg ggttagcgct tcgtgatgcg cagtgcgcgc agcagagagg
     4741 cgccgaagcc gcgaccgtcg cgctcgcgga tcggctccgt cgagtgggtg cggcggcgtg
     4801 cttcgtagag catctcgttc tgccgcagat ttgcgagcag gccgagataa gttgctcctc
     4861 ggctcatggg aaacctcctg gtgtgtggaa atcgcgagcg ttgctcgaga ctgatttgtc
     4921 agacgtgatg cgaggggggg tcgccggtgg tggtgcggct atcggccccc cagacgaccg
     4981 cggcaaggtc aaatgcgacc agaaccgtta ggacgatcaa gaaaccaacc attgttctgc
     5041 gcctcccctc gtgggacctg taactgttag atgccatatg aaaggttaca gtgagactgt
     5101 acccccgtcc aagtaacttg tcaagcgctg tatgatggtt acatggtact ggacagaacg
     5161 acgcttcccg agcccgacca ggccgaggag ctcttcattt ccttcggcaa cacgctcgaa
     5221 tttgagcatg gccagccggt ggatgccgtc cccgacatag cctcgctgct ggcctggctg
     5281 cgcgagcgac gcctgatcag cggccggggc ctcgcggtcg aggccgctca ccttcgacgg
     5341 gacctcgacg agaccgcgcg tcgcgtcgac cggtttcacc acctgcgcaa cctgctgcac
     5401 gaggtcgccg aggaggtgac cgaggccggc caccctacga ccgagcagct gcgcgacctc
     5461 aaccacatcc tgcgtcacgg gctccactac caccagctcg agcgagatcc agacgggact
     5521 cggtacacct ttgcccaggt gggggaccgg ctcgaccagg cacgagccac catcgccagc
     5581 tcgcttgcac agttcctggc cgatgacgcc ccctctcgcc tccgcgtgtg cgccaatgcc
     5641 ggctgccggt acctgttcgt cgatcgctcc ccgaccggca ggcgacggtg gtgcgacatg
     5701 cgcacctgcg gcaaccaggc caaagtcgcg cggcatcgcg ctcgcgcgcg cgaccagctg
     5761 catgagacgt cggcgagcgt ttcctgaggg gttccgcgag caccgggcgc ggtctagaat
     5821 caggcggact cgaccgcggc ttcggccagc ggtcgtgccg tggagaacgc accgaggcgt
     5881 agcacgatcc caggtggatg ccggttccga tagccgaatc tgcgcgcgcc cgaggcccgg
     5941 ggcgagggtg accgcctagc ggcgaccccg accacccgcg cgcgtcacgt cacatccgac
     6001 gccagagcga ggtggtcgtt tcatgctctt cctcagccgg ctggtcggcc gtccggtacg
     6061 cgatcggcac ggcgaatccc tcggcaaggt ccgcgacctg atcgtggccc tcggcgagcg
     6121 gtatccgccg gtcaccggcc tggtggtgcg cgtcaccgct ggccgcgaca tcttccttcc
     6181 ctggaccgat gtcgaggaga tcgacgccga cggcgcacgc ctgcgcacca cgctcatcga
     6241 catggagagc ttccgccagc gcccggacga gatccgcctc tggctcgacc tgctcgacaa
     6301 gcagatcgtg gacgtcgagg gtcgcaagat cgtccgcgtc aacgacatcc agctcgcgcc
     6361 cgtcgacggg cgactgcgcc tcgtggcggt tgacgtcggc ctggcgggca tcctgcgccg
     6421 gctgggcctc gccggtcccg gcgagcggtt ggcccgagcg ctgcacctcg aggcggagaa
     6481 ctacatcgag tgggaggacg tggaccctgt cgagtcgagc gtctcgagcc tgaagctgcg
     6541 ggttccgcac gaggcccttg gcacgctcca ccccgcggac gtggcccaca tcgtcgagca
     6601 gcttgcgccg cgcgaccggg ccgggatcct cgccgccctc gacgacgaac gagcggccga
     6661 cgtgctcgag gagctggccg ctgacgacca ggtcgacatc ctcgaggcgc tcgagcccga
     6721 gaaggcagcc gacatcctgg aggagatggg gcccgacgaa gcggctgacc tcgtcgccga
     6781 cctgtcagag gagcgacagg agcagctgct gggcctgatg gagccggagg aggcatccga
     6841 cgtccgcgag ctgctcgcct acggcgagga gaccgccggc ggcctgatga ccaccgactt
     6901 catcacggtg acgcccgaca tgaacgccga gcaggtcatc gagcgactgc gggacctcca
     6961 tccggacgcc gaccaggtct actacctgta cgtgaccgat gccgacgggg tgctggtcgg
     7021 cacgatcacg ctccgtggcc tgatcgtcgc ggtgccggag acggcggtgc gtgacttcat
     7081 gcggtccgac ccgatcagcg tgagtgtcga cctggacgcc gaagaggtgg gggccgcgat
     7141 cgcgcgctac aacctgctgg cgctgccggt ggtcgacgcg cagggtcgga tgcaggggat
     7201 cgtgaccgtg gacgatgccc tcgagcgcgc gctgggtgag gggtggcgca agcgcctccc
     7261 ggagatctac gagccggccg aggaccggta ggcgccacat gacccggcgc ttggccctcc
     7321 cacgcccgat ccggcgcgcg cagcgctcgc tccgtgagcg cctgccgaac ttccgagtcc
     7381 atcggtccgg gctgctggcc ttcctgggcg tgatgggtcc ggggctgatc gccgggctgg
     7441 cagggaacga cgcaggtggc atcgcaacct atagcgtgct cggcgcggag accggcctct
     7501 ccctgctctg gatcttcccg atcaccacga tcctgctcgt ggtcgtgctc gagatggcag
     7561 cgcgcatggg ggccgtctcg ggtcagggac ttggcgacct gatccgggac gatttcggca
     7621 tccgctggac gctcttcgcc atgatcgtgc tgctgatcgc caacggggcg aacatcgtgg
     7681 ccgagttcgc gggagcggcc gcggccctgg agattttcgg catctcccgc tacgcaaccg
     7741 tcccgatcgt ggcggcgggc atctgggcgc tggtcgtctt cgccagctat cgcgccgtgg
     7801 agcgggtctt cctgtcagtg gcggtcgtct tcggggccta cgtggtgagc gccttcgtgg
     7861 cggggccgga ctggggagag gtggggcggg ctctcgtctc gccatcggtc tcgctggctc
     7921 ctcacgccct gctgctgatg gtcgccaccg tgggcaccac cgtcacgccg tacatgttct
     7981 tctacctgca gtcgtcggtc gccgacaagg ggctgggacc ggaggagctg cagttcgagc
     8041 gcgccgacgc tgtgctgggc ggcgtgtgga ccaacgccat cgccctcttc atcgtgatcg
     8101 tgacggccgt caccatcttc ccccgcggca tcaccatcga aagcgcggac caggcggccc
     8161 tggcgctcgc gccggtcgcc ggcgacttcg ccaaggggct cttcgcgttt ggtctgttcg
     8221 gggcgtcggt gctggcggcg acggtcatgc cgctgaccac gagctacgcc gtttgcgagt
     8281 ccttcggatg ggagtcgggc gtgtcgcgca agttccgcga ggcgcccgcc ttcatgggcc
     8341 tctacaccgc cctgatcgtg atcggcgcgg tcgtcgtgct catcccggga ctgccattga
     8401 tcggggtcat cctcatcagc cagaacctca acgggatcct gctcccgatc atcctcgtct
     8461 tcatgctgcg actggtgaac aacccgcgga tcatgggcga gcacaccaac ccgcgctggc
     8521 tgaacgtgct ggcctggggg ctgaccgggt tggtgatcct gctgacgggc gtgctcttcg
     8581 gctcaaccct tgccagctgg ctcggcggag gctagcccga tgggcccggg ccgaggctag
     8641 gcgccgatcg ggtgccagac cgtcttggtc tcgacgaact ccccgatgag gtacggggac
     8701 tgggtgcgcg ggtccagcca gtcgacggcg tccgctccgt tggtcggcgg gcgcacgagg
     8761 cgcttgacgt tctcggtggc cagctcctcg acggcaatcc cctgcgccgg gtcgagcccg
     8821 taggcgtcca gggcgttgac gtccatgtgc ccagccaggt gcagcaccag ctcgcggcgc
     8881 aggcccgtga tcaggttgac caccccgccc ggcacgtcgg aggtggccag gacctcggtg
     8941 agcgtcaccg ccggtagcgg gcgtgactca gaggccagca ccaccgcaca gttgccggac
     9001 acgatgacgg gagccaggcg acttaccagc ccgaggaggc tcgaggcctc gggcgcgatc
     9061 accccaacaa cgccggtcgg ctcggggacg ctgaagttga agtagctgcc gttgacgggg
     9121 ttgaccgagc cgttcacctg gctgaacttg tcggcccagc cggcatacca gacccatcgg
     9181 tcgatggcgg cgtcggtgag gcgggtggcg cgctgccgcc ccatgccctc ggcggccatc
     9241 acctcgctga cgaactggtc ccgccgtcct tccatc
//
LOCUS       DATCQR010000101         3477 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_36665, whole genome shotgun sequence.
ACCESSION   DATCQR010000101 DATCQR010000000
VERSION     DATCQR010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3477
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36665"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(203..1219)
                     /locus_tag="VIN32_03410"
     CDS             complement(203..1219)
                     /locus_tag="VIN32_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8921661.1"
                     /translation="MASVTYDHVTKTFGDTAAVKDLMLEVQDGEFMVLVGPSGCGKST
                     ALRMLAGLEKVSDGRILIGDRVVNNVAPQSRDIAMVFQSYALYPHMTVYDNLAFGLRN
                     QNVPKKDIDRRVRRAADILDLGSLIKRKPKQLSGGQRQRVALGRAIVREPEAFLMDEP
                     LSNLDAKLRVQTRAEILKLQQELGTTTIYVTHDQVEAMTMGDRIVVMDHGVLQQAGTP
                     EELYTQPRNLFVAGFIGSPAMNLLPAEKLNGSGSGRVAGFRPEHVELGHPGTDAVQLT
                     GRVEVREYLGDEQLVHLSVKDTPILAKIPVEEHVSPGDEVSFSIRRDKIHLFDAESGE
                     RAGS"
     gene            complement(1224..1868)
                     /locus_tag="VIN32_03415"
     CDS             complement(1224..1868)
                     /locus_tag="VIN32_03415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921662.1"
                     /translation="MSLPRALGVAAADLYHQSWRLVVLNVLLGSVLIGVVLASLAIRP
                     AILLVVLLGPAAAAVMHCAVTLAQTEELRLSEAVVGLRRHWRRGLALGTIVLAAAILG
                     AIAVPFYSRAGTWAWPLAALSVYVLLVFALFQLALWPLAVFEDARPFRDVVRDAALTV
                     GRRPWGFTGLALALLLVNAIGVAAAILPFLTLTIAYSFLVSAHFALPKNPAREV"
     gene            complement(1865..2836)
                     /locus_tag="VIN32_03420"
     CDS             complement(1865..2836)
                     /locus_tag="VIN32_03420"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEY8921663.1"
                     /translation="MAGERAPPTPPVLAVGELAVEGEAAASTGGGSAGIPGRERADRI
                     RIVLTYTVLFTVAALFAIPFLWSVSTSFRTIAETVQGFSLLPHHWTTIGYHEVFYKYH
                     FGRYTMNSAIIAGAVTTSNLFLASLGGYAFARLRFPGRELLFMLVLGTLMIPDQLRLV
                     PIYQMLVNMVPSWLGHVGWHNATFVNRNGVILISLVSASSLFLMRQYFLTIPRDLEEA
                     AKLDGAGYFKTYRRVMLPLAGPALAAVGILTFQGSWNGLFWPAVLLQDESQHTIPLGL
                     VYFQQVYTTLWPELMAASVTAILPILVLFVLFQRYFVAGVAASGVKG"
     gene            complement(2836..>3477)
                     /locus_tag="VIN32_03425"
     CDS             complement(2836..>3477)
                     /locus_tag="VIN32_03425"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEY8921664.1"
                     /translation="LQMALGLALAIVVNQAIRFRTFFRAAFYFPSLASSAAIVSIATY
                     ILASDGLLNSFLGHLGYQHHNAWFFQQSTALPSIMGLNAWTTSGTMMLFYLASLQAIS
                     TEVYEAAAIDGAGAWRTFRKITFPLLKPGHFFVAVVSIIGALQMFDQSFIVGGSSGGP
                     NYSTMTVVLYLYNRAFTGTTRFGYAATVGLVLFVFIFTVTLIQRLLFGKAEVS"
BASE COUNT          685 a         1105 c         1168 g          519 t
ORIGIN      
        1 ggtgatcacg tcgacgccgg cctgctccat gtccttgacc gcgagccgcg tcgcgtcgtc
       61 ctgggcctcc tcgagcaacg gctccggtac ccgccagagc tcacgcgccc gcacgcgcgg
      121 cggcagccgg tctccgagcc gttcccggtc gatcagccag ttgggctggg gatagctccc
      181 gacgaccgtc gtgaccggag catcatgaac cagcgcgctc gccgctctcg gcgtcgaaga
      241 ggtggatctt gtcgcggcgg atcgagaagg acacctcgtc gcccggtgag acgtgctcct
      301 cgactgggat cttcgccagg atcggcgtgt ccttgacgct caggtggacg agctgctcgt
      361 cgccgaggta ctcccgcact tcgacgcggc ccgtcaactg cacggcgtcg gtgccggggt
      421 gtccgagctc cacgtgctcc ggccggaagc cagcgacgcg cccggaccca gagccgttca
      481 gcttctcggc cgggaggagg ttcatcgccg gcgagccgat gaagccggcg acgaagaggt
      541 tcctcggctg cgtgtagagc tcctcgggcg tgccggcctg ctggaggacg ccgtgatcca
      601 tcacgacgat ccggtcgccc atcgtcatgg cctcgacctg atcgtgggtg acgtagatgg
      661 tggtcgtccc gagttcctgc tgcagcttga gaatctcggc acgagtctgg acacgcagct
      721 tcgcgtccag attcgacaag ggctcatcca tgaggaaggc ctccggctcg cggacgatcg
      781 cgcgcccgag cgccactcgc tgccgctggc ctccggagag ctgcttcggc ttgcgcttga
      841 tcagcgaccc gagatcgaga atgtccgcag ccctccgcac gcgccggtcg atgtccttct
      901 tgggcacgtt ctggtttcga aggccgaacg cgaggttgtc gtaaacggtc atgtgcggat
      961 acagcgcata cgactggaac accatcgcaa tgtcccgcga ctgcggcgca acgttgttga
     1021 ccacccggtc gccgatcaag atgcggccgt ccgagacctt ctcgagacct gccagcatgc
     1081 gcagcgccgt gctcttgcca cagcccgatg ggccgacgag caccatgaac tcgccgtcct
     1141 ggacttcgag catcaggtcc ttgacggccg cggtatcgcc gaaggtcttc gtgacgtggt
     1201 cgtacgtgac gctggccatg cgctcagacc tcccgggctg gattcttcgg cagcgcgaag
     1261 tgcgccgaca cgaggaacga gtacgcgatc gtcagggtca aaaacggcag gatcgctgcc
     1321 gcaacgccga tcgcgttgac gagcaacagc gccagcgcaa gcccggtgaa cccccagggg
     1381 cggcgcccga cggtcagcgc ggcgtcgcgc acgacatccc ggaacgggcg cgcgtcctcg
     1441 aaaaccgcca gcggccacaa cgcgagctga aagagagcaa agacgaggag gacgtagacg
     1501 ctcagcgcgg caagcggcca cgcccaggta cccgccctgg aatagaaggg gacagcgatc
     1561 gcaccgagga tcgcggccgc caggacaatg gtgcccagcg ccaacccgcg tcgccagtgg
     1621 cgacgaagtc caacgactgc ctcggacaag cgaagctcct ccgtctgggc gagagtcacc
     1681 gcgcagtgca tgacggccgc ggccgcaggc ccgagcaaga cgacgagaag gatcgccggc
     1741 cggatcgcca ggctcgcgag aacgactccg atcaagaccg agccgagcag cacgttcaac
     1801 acaacgagcc gccaggactg gtgatagagg tcagccgctg cgaccccgag cgcgcgcggc
     1861 agactcatcc cttgacgccg gaagcggcga cgccggcgac gaagtagcgc tgaaacagga
     1921 cgaacaggac gaggatcggc aagatcgcgg tgacgctcgc ggccatcagc tctggccaca
     1981 gggtcgtgta gacctgctgg aagtagacca acccgagcgg gatcgtgtgt tgcgactcgt
     2041 cctgcaggag gaccgccggc cagaagagcc cgttccacga gccctggaag gtcaggatcc
     2101 ctacggcggc gagcgccggc ccggccagcg gcagcatcac gcgccggtag gtcttgaaat
     2161 agcccgcccc gtccagcttg gccgcttcct ccaggtcgcg gggaatcgtc aggaagtact
     2221 gtcgcatcag gaacaggctc gacgcggaga ccaggctgat caagatgacg ccgttgcggt
     2281 tgacgaaggt ggcgttgtgc cagcccacat ggccgagcca gctcggcacc atgttgacga
     2341 gcatctggta gatcggtacg aggcgtagct ggtccgggat catcagcgtc ccgaggacga
     2401 gcatgaagag cagctcgcgg ccggggaacc tgagccgcgc gaacgcgtag ccgcccagag
     2461 atgcgaggaa gaggttcgag gtcgtgaccg cgccggcgat gatcgcgctg ttcatcgtgt
     2521 agcggccgaa gtggtactta tagaaaactt cgtggtagcc gatcgttgtc cagtgatgcg
     2581 gcaagagtga aaaaccttga acggtctccg caatcgtcct gaaagacgtg gacacactcc
     2641 agaggaacgg aatcgcgaac aaggctgcca ccgtgaacaa cacggtgtag gtgagaacga
     2701 tccggatccg gtcggcgcgc tcgcggccgg ggatgcccgc cgagccgccg ccggttgagg
     2761 ccgcagcctc cccctccaca gcgagctccc cgactgccag gacaggtgga gtgggcggcg
     2821 ctcgctcgcc ggccatcagg agacctccgc cttgccgaac aggagccgct ggatgagggt
     2881 gacggtgaag atgaagacga agaggacgag tccgactgtc gcggcgtatc cgaacctggt
     2941 ggtgcccgtg aacgctcggt tgtagaggta gaggaccacc gtcatcgtgg agtagttcgg
     3001 tccgccggat gagccaccca cgatgaagga ctggtcgaac atctgcaacg cgccgatgat
     3061 cgagacgacg gcgacgaaga agtgtcccgg cttcaagaga gggaacgtga tcttcctgaa
     3121 cgtcctccac gcgccggcgc cgtcgattgc agcagcttcg tagacctcgg tcgaaatcgc
     3181 ctggagcgag gcgagataga agagcatcat cgtccccgac gtcgtccagg cgttcagccc
     3241 catgatcgaa ggcagcgcgg tgctctgctg gaagaaccag gcgttgtggt gttggtaacc
     3301 gaggtggccg aggaacgagt tcaacaatcc atcggacgcg agaatgtagg tcgcgatcga
     3361 cacgatcgca gccgaggatg cgagcgaggg gaagtagaac gccgcccgaa agaacgttcg
     3421 gaagcggatc gcctggttca cgacgatggc cagcgccagg cccagcgcca tctgcag
//
LOCUS       DATCQR010000102         5428 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_160796, whole genome shotgun sequence.
ACCESSION   DATCQR010000102 DATCQR010000000
VERSION     DATCQR010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5428
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160796"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="VIN32_03430"
     CDS             complement(<1..443)
                     /locus_tag="VIN32_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY8921665.1"
                     /translation="MTGTLLTTLEVAERLGLSSAAVAHAVRTGALLPTARTNEDFLFT
                     ERAVAAFAESRADAAATPLEPPPAVSRGEWSGDVDRLNTWLKDLSESAPGAAAAAAAA
                     AAPPAPPDAHAAPAAPAAPVPAPVPIATPEEARPAIDFRPPPHPVA"
     gene            complement(448..1107)
                     /locus_tag="VIN32_03435"
     CDS             complement(448..1107)
                     /locus_tag="VIN32_03435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921666.1"
                     /translation="MSRPRESEVLRQVAQRLAEVFAAAAAVVTRSEAEIRRHEEEAAR
                     VANVLGKADPASRGRVMSARRAAGMAELLAAQTLADEMRYQAGSRALAAVHRYAGTLA
                     AEAEAANAIGRSKLAELETVFDGLDSTNARALLAVVEEAEAQRIANAERRAADLTAQI
                     QAAMGSLPPPPVAFDVTRPRSGKARRRARRNQVEAQPQDAEADSADVVRLNPNRRRSV
                     T"
     gene            complement(1124..1831)
                     /locus_tag="VIN32_03440"
     CDS             complement(1124..1831)
                     /locus_tag="VIN32_03440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921667.1"
                     /translation="MADLASSVPSAHSAAIYRYAVELVEDTRRANETRMADARDLASV
                     VRIADEPAADRILGAAEEACARDLHIAEQQAEEVIRHARAGIMAAVQRYAQALIEDAR
                     NAGQSRLDKATELAELVRLADANSADRLLQAIHTVNEAALRTAERQSQGVLETAQTRL
                     AMAGRLAETSAYSSQTGTAPPVPAADPAAAAGYADTVQTQAPSFSQAPTNGAPAPAAE
                     PAGEEADYSRYRPEESQ"
     gene            2001..2831
                     /locus_tag="VIN32_03445"
     CDS             2001..2831
                     /locus_tag="VIN32_03445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921668.1"
                     /translation="MGKKRAQVRQVKKTMRQVRPKPSAGTSGGPQQRKQRERYVEAGG
                     FLQGYSPEFVIRLGYYSIAAVVVSLLIMAELIFGPLAPHGTAVRIVAAAAWLAPVVIL
                     VSLVAPGVRLAFRDRSAQPLVVQGQLMGASSVSTSRGLGMLMIRTRTGTEQYLCPPEK
                     LTKVPGNVVQVALSVTPNLRYVRSVSVMGQRQMARSEPPVPTVLRRLQLLPLATPAAL
                     TLAFVLGDDVTAFIPLTPDALHAVAAAVVGVALGGAVFGTSYLYQKRLMAEAQAMVPA
                     "
     gene            complement(2905..3321)
                     /locus_tag="VIN32_03450"
     CDS             complement(2905..3321)
                     /locus_tag="VIN32_03450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921669.1"
                     /translation="MNSMLKARVLTVVMLLLGILMVVGSTMSIFSTSVFPFDGLAGTD
                     ASVAGVAFGVGIAVASFNPERHVTWVRAAILYAALVVVYRIIFGLAWGTWGQPAPLVL
                     AIIFGLVLIFLYPRRSELMPTSGAMTDSPVRPVASH"
     gene            3396..3863
                     /gene="greA"
                     /locus_tag="VIN32_03455"
     CDS             3396..3863
                     /gene="greA"
                     /locus_tag="VIN32_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016998523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEY8921670.1"
                     /translation="MPPDVDITPEGLEAVRAELDQLTTVRRPNIVEKIKSARELGDLS
                     ENFEYHSAKNEQGFIESRIQELEQIIRNHKLITAPTATGVVQLGNTVRFSEEDGPEES
                     YRIVGPAEADPGHGRVSNESAVGKALLGRKVGELATVETESGDSYKLKVLGIE"
     gene            complement(3875..4801)
                     /locus_tag="VIN32_03460"
     CDS             complement(3875..4801)
                     /locus_tag="VIN32_03460"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEY8921671.1"
                     /translation="MSEHRHLDLTGNALRQAFLLTVLILVVEAVAGIFSHSLALLADA
                     GHILTDVVALGLAWFAVVQARRPADAQRTYGYHRVGILAAMANGATLILIVVAVAFEA
                     ARRFAHPEPVNGGLVVASALLAVGVNTFIALRLRGNQRNLNVRAALLHVLGDLAASIG
                     VIVAGLVILLTGWLYADPIVSLGITGLIAFSAIRIVLDTMNVLLEGVPNGIDLEDVRR
                     TVAAAPGVSSVHDLHIWSLSGEQIALSCHVVVPEELLAADSEHLVRRLEEAICGKFGI
                     GHTTIQVEACHPCDETLGHGVGDHNHPHAVGR"
     gene            4835..>5428
                     /locus_tag="VIN32_03465"
     CDS             4835..>5428
                     /locus_tag="VIN32_03465"
                     /inference="COORDINATES: protein motif:HMM:NF013486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family N-terminal
                     domain-containing protein"
                     /protein_id="HEY8921672.1"
                     /translation="MSIQRLREWMESTHVAAACVTDPTSIAYLTGFYANPHERLLALA
                     VRPGGAVLLVPDLERQNAEGRATGVEILTWQDGQDPFRLLRGALGDPPLLAVEKEHLT
                     IARWERLDAGAIEDCSEALRAMRAVKLPRELELLQRAAQLTDEVTDAILAELRAGPTE
                     REVATRLNALINETGSGLAFGSLVQAGPNSALPHLPPG"
BASE COUNT          876 a         1801 c         1921 g          830 t
ORIGIN      
        1 gccacggggt gagggggcgg ccgaaaatcg atcgccggtc gcgcttcctc gggcgtcgcg
       61 atcggaacgg gcgccgggac gggcgctgcc ggggccgccg gcgcggcgtg tgcgtccggc
      121 ggggccggcg gcgcagccgc cgcagccgcc gcagccgccg ctccaggggc cgactcgctg
      181 agatctttca accatgtgtt gaggcggtcg acatcgcccg accattcacc gcggctgacc
      241 gccggaggtg gctccaacgg cgtggccgcg gcgtcggccc tgctctccgc gaatgcggcg
      301 acggcacgct cggtgaagag gaagtcctca ttcgtccgcg cggtcggcag gagtgcgcca
      361 gtccgtaccg cgtgggcgac cgccgcactg ctgagaccga ggcgctcggc aacctcgaga
      421 gtcgtcagca gcgtgccggt cattgaatca ggtcacgctg cggcgtcggt tcgggttcag
      481 gcggacaacg tcggccgagt ctgcttcggc gtcctggggc tgggcttcca cctggttgcg
      541 cctggccctg cggcgggctt tgcccgagcg tggtcgggtc acgtcgaacg cgaccggggg
      601 cggcggcagc gagcccatcg ccgcctggat ctgcgcggtg aggtcggcag cccggcgctc
      661 ggcgttggcg atccgctggg cctccgcctc ctcgacgacg gcgaggagcg cccgggcgtt
      721 ggtgctgtcg agcccgtcga acacggtttc gagctcggcc agcttcgaac gtccgatggc
      781 gttggccgcc tccgcctcgg ccgcgagcgt gccggcatag cggtggacgg ccgcgagggc
      841 gcgcgatccg gcctggtagc gcatctcgtc ggcaagggtt tgggcggcca ggagctcggc
      901 catgccggcg gcgcggcggg cggacatcac ccggccgcgg gaagcgggat cggcctttcc
      961 gagcacgttt gcaacgcgag cggcttcctc ctcatggcgg cggatctcag cctccgacct
     1021 ggtaacgacc gcggcggcgg ccgcgaacac ctcggccagc cgctgcgcca cctggcgcag
     1081 cacctcgctc tcccgtggtc ggctcactgg ctgactcagg aagctactgc gactcctcgg
     1141 gccggtagcg gctgtagtcg gcttcctctc cagccggctc ggccgcgggc gcgggcgcgc
     1201 cgttggtagg cgcctgggag aagctggggg cctgggtctg gacggtgtcc gcgtacccgg
     1261 ccgcggccgc cgggtcagcc gccggaaccg gcggcgcagt ccccgtctgg ctggaatagg
     1321 cgctggtctc agcaagccgt ccggccatgg cgagccgggt ctgtgccgtc tccagcacgc
     1381 cctgcgactg gcgctcggcg gtgcggaggg cagcctcgtt cacggtgtgg atcgcctgga
     1441 gaaggcgatc ggccgagttg gcgtcggcga ggcgcaccag ctcggcgagc tcggttgcct
     1501 tgtcgaggcg gctctggccg gcgttgcgcg cgtcttcgat cagggcctgc gcgtagcgct
     1561 ggacagcggc catgatcccg gcacgagcgt gccggatgac ctcttcggcc tgctgttcgg
     1621 cgatgtgcag gtcgcgtgcg caagcctcct cggcggcccc caggattcgg tcggcagccg
     1681 gctcgtcggc gatccgcacc accgaggcga ggtcgcgtgc gtccgccatg cgggtctcgt
     1741 tggcccggcg ggtgtcctcg acgagctcga ccgcatagcg ataaatggcg gccgagtgtg
     1801 cgctcgggac tgacgatgca aggtcagcca tgggaattga aagctcctcg attcttattg
     1861 tgaagatggc ggtaatgccc gccaatggcg acagctcacg aaagcctagc ccaatttgat
     1921 gcgaaacgga cggcttcggc aaagagcgcg gacgagcctg ccggagcatc agccacggtc
     1981 gaataaactt cgtccgcggc atgggaaaga agcgggcgca ggtgcgacag gtaaagaaga
     2041 cgatgcggca ggtgcgcccg aagccttcgg cgggcacttc gggaggccct cagcagcgca
     2101 agcagcgcga gcgctacgtc gaagcgggtg ggttcctcca gggctattcg ccggagttcg
     2161 tcatccggct cggctactac tcgatcgccg cggtggtcgt gagcctgctg atcatggcgg
     2221 agctgatctt cggacctctc gccccccacg ggactgccgt ccggatcgtg gcggccgccg
     2281 cctggctggc ccctgtcgtg atcctcgtca gcctggtcgc gccgggggtc cggctggcgt
     2341 tccgggaccg ctcggcgcaa cccctggtcg tccagggtca gctgatgggc gccagctcgg
     2401 tcagcacctc gcggggtctc ggcatgctga tgatccggac ccggaccggc accgagcaat
     2461 acctctgccc gccggaaaag ctgacaaagg tccccggcaa cgtggtccag gtcgcgctca
     2521 gcgtcacccc caacctccgc tacgtgcgct cggtctcggt gatggggcag cgccagatgg
     2581 cacgctccga gcctccggtg ccgaccgtgc tacggcgatt gcagctgctg ccgctggcga
     2641 caccggccgc cctcacattg gccttcgtgc tcggcgacga cgtgaccgcc ttcatcccgc
     2701 tgacccccga cgcgctccat gcggtggcgg ccgcggtggt tggagtggcc ctcggagggg
     2761 ccgtgttcgg gacatcctac ctctaccaga agcgcctgat ggccgaggcc caggcgatgg
     2821 tccccgccta gaaacgccac ataaataaag aagaagggga gcccagcggc tccccttctc
     2881 aggtcagttg gtgtggtctg gttttcagtg agaggcgaca ggcctgacgg ggctgtcggt
     2941 catcgcgccg gaagtcggca tgagctcact gcggcgcggg tacaggaaga tcagcaccag
     3001 gccgaagatg atggccagta cgagaggcgc gggctggccc caggtgcccc aggcaaggcc
     3061 gaagatgatg cggtagacga caaccagggc cgcatacagg atcgcggcac ggacccaggt
     3121 gacgtgacgc tcggggttga acgaagcgac cgcgatgccg acaccgaagg cgacgccggc
     3181 aaccgaagca tcggtgcctg cgaggccgtc gaatggaaat accgatgtcg agaaaatcga
     3241 catcgtgctg ccgacgacca tgaggatccc cagcagcagc atcaccaccg tcagcacgcg
     3301 agccttcaac atcgagttca tagacgttcc tccttggatg gacgggcgct ttgcgcgcac
     3361 agcataagta ctaacggcac tcgtagactg aacttgtgcc gccggatgtt gacataactc
     3421 cagaaggttt ggaagcagtt cgtgcagaac tcgatcaatt gacgacggtt cggcggccaa
     3481 atatcgtcga gaagatcaag tccgcgcgcg agctcggcga tctctccgaa aacttcgaat
     3541 atcactccgc gaagaacgag cagggcttca tcgaaagccg gatccaggag ctcgagcaga
     3601 tcatccgcaa ccacaagctg atcacggctc cgacggcgac cggcgtcgtc cagctcggca
     3661 acacggtgcg tttcagcgag gaggacggcc cggaggagtc gtaccggatc gtcggaccgg
     3721 ccgaggccga cccgggccac ggcagggtca gcaacgagtc ggcggttggc aaggccctcc
     3781 tgggccgcaa agtaggcgag ctggccacgg tcgagacgga gtcgggggac agctacaagc
     3841 tcaaagtcct ggggatcgaa tagaggctag cccgtcacct accaaccgca tggggatggt
     3901 tgtggtctcc cacgccatgc cccagcgtct cgtcgcaggg atggcacgct tcgacctgga
     3961 tcgtcgtgtg gccgatcccg aacttgccgc agatcgcctc ttccaatctc cgcacgaggt
     4021 gctccgaatc ggccgccagc agctcctcgg ggaccacgac gtggcagctg agggcgatct
     4081 gctcgcctga gagcgaccag atgtgcagat cgtggaccga gctgacaccc ggcgccgccg
     4141 cgacggtccg gcggacatcc tccaggtcga tgccgttcgg gacgccctcg aggaggacat
     4201 tcatcgtgtc gaggacgatc cggatcgcgc tgaacgcgat cagcccggtg atcccgagcg
     4261 agacgatcgg atcggcgtag agccacccgg tcaggaggat gacaagcccg gcgacgatca
     4321 caccgatcga cgccgccagg tcgcccagga cgtgtaggag ggcagcccgg acgttcaggt
     4381 ttcgctggtt gccgcgcagc cgcagggcga tgaaggtgtt taccccgacc gccagcaggg
     4441 cggaggcgac gaccaggccg ccgttcaccg gttccgggtg ggcgaaccgc cgggccgcct
     4501 cgaaggcgac cgcgacgacg atcaggatca gggtggctcc gttcgccatc gcggccagga
     4561 tgccgacccg gtggtagccg taggtgcgct gcgcgtccgc cggccggcgc gcctggacaa
     4621 cggcgaacca ggccaggccg agggcgacca cgtcggtcag gatgtggccg gcgtcggcga
     4681 gcagcgccag tgagtgcgag aagattccgg cgaccgcctc gacgaccagg atgaggacgg
     4741 tcaacaggaa cgcctggcgc agcgcgttcc cggtcaggtc aaggtgtcgg tgctcgctca
     4801 attgtctact cgaaccaact gtaccctcgt gtcgatgagc atccaacgcc tgcgggaatg
     4861 gatggagtcg acccacgtcg cggctgcctg cgtcacggat ccgacctcga tcgcctacct
     4921 gaccggcttc tatgccaacc cgcatgagcg gctgctcgcg cttgcggtcc ggcccggggg
     4981 cgcggtcctg ctggttcccg acctcgagcg ccagaacgcc gaaggccggg cgaccggggt
     5041 cgagatcctc acctggcagg atgggcagga ccccttccgg ctcctccgcg gtgcgctcgg
     5101 cgatcccccc ttgctcgcgg tcgagaagga gcatctgacg atcgcgcgct gggagcggct
     5161 cgacgcggga gcgatcgagg actgttcgga ggcgctgcgc gcgatgcggg cggtgaagct
     5221 gccccgcgag ctggagctgc tgcagcgagc ggcgcagctc accgacgagg tgaccgatgc
     5281 catcctcgcc gagctccgag ccggcccgac tgaacgggag gtggcgacgc ggctcaacgc
     5341 cctcatcaac gagaccggct ccgggctcgc cttcgggtcg cttgtccagg cgggtcccaa
     5401 ctccgccctg ccccacctgc cgccgggc
//
LOCUS       DATCQR010000103         4454 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_145326, whole genome shotgun sequence.
ACCESSION   DATCQR010000103 DATCQR010000000
VERSION     DATCQR010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4454
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145326"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="VIN32_03470"
     CDS             <1..276
                     /locus_tag="VIN32_03470"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEY8921673.1"
                     /translation="VGRERVVMLRDPCDLPIIGPLLNLVPLARNFSRSDHRRFWDARL
                     PAIQVTNTANFRNPNYHRAGDKPATLDYETLADITVATVLAVRRLAG"
     gene            314..730
                     /locus_tag="VIN32_03475"
     CDS             314..730
                     /locus_tag="VIN32_03475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921674.1"
                     /translation="MDKLFAPFNPTNFGDLFTMMWVGAFVILAGAVIVYNTAQRMYRR
                     YPAILALHEWIFWAIFGTWLVVPLLVVIGVPLALLLLLIVPGIAIATYAAFVKFPPII
                     EAANVEIRRRRFVPPPRRDVRERPKPTPAGRRRRHR"
     gene            complement(727..1476)
                     /gene="arsM"
                     /locus_tag="VIN32_03480"
     CDS             complement(727..1476)
                     /gene="arsM"
                     /locus_tag="VIN32_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006126391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HEY8921675.1"
                     /translation="MTDQIHETVRERYAQSALQVLARGENTSCCGDACCTDTSVGPDL
                     YSALEREELPSAAVMASLGCGNPIAVADLHPGERVLDLGSGGGIDVLLSARRVGPTGR
                     AFGLDMTDEMLTLAQRNAAEAGAGNVEFLKGQIEAIPLPAESIDVVISNCVVNLAADK
                     PSVFREIARVLRPGGRIGISDIVADDMLTAEQRAERGSYVGCIAGALSFGEFEAGLRE
                     VGLTDVSVTPTHSVADGMHSAIIRATKPASA"
     gene            complement(1518..1907)
                     /locus_tag="VIN32_03485"
     CDS             complement(1518..1907)
                     /locus_tag="VIN32_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921676.1"
                     /translation="MPKKQPNTASELLADWRSAGRDTAAAKAASKVANLALQAAMAAD
                     EAASETEAAARAAASAVDQALKAAASAKRAAERAATAARILIASAEGDQVRANQDLEQ
                     AEDAEESAGDEFHEAEKLGFPRDGESS"
     gene            complement(1980..2183)
                     /locus_tag="VIN32_03490"
     CDS             complement(1980..2183)
                     /locus_tag="VIN32_03490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921677.1"
                     /translation="MATLFGLVGRFTGKLLTMSLGWASLLPFGRVPRDKEIFLAAITF
                     GSVGNASDRGARQTGQARHGLRV"
     gene            complement(2194..3153)
                     /locus_tag="VIN32_03495"
     CDS             complement(2194..3153)
                     /locus_tag="VIN32_03495"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HEY8921678.1"
                     /translation="MGIRSRLYDARGEDKEIELSATAVAELTDETLLWVDIDAPDQAA
                     LEQIEAAFRFPAPIMTALATPGDEARLVRAPAAILLTVLSIEDRDGRDFPVAVDLVAG
                     RNAVVTLHASPVAALDEFESHVSDEQRLGSLDAGSLVGGLVDTMLGAFRRQVEEIEEQ
                     VDRLDEIALRGEDRADFLERVVALRRRAAKLRRILVPQRETFGPLSRPDFALHEDLGK
                     PWPGLLDRLEQAISGVERTRELLVGSSDIYLGRMAQRSGDVMKALTILSAVLLPAVVL
                     AGVMGMNFKLGFFDDPSHFWLVLAGMLGLAVLILGLARFRHWI"
     gene            complement(3232..>4454)
                     /locus_tag="VIN32_03500"
     CDS             complement(3232..>4454)
                     /locus_tag="VIN32_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012306065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY8921679.1"
                     /translation="REQLEKLTPEIEAAERAADYARAAELKYGRANELATQLAEQEGR
                     LKELKASGSLMLKEEVDADDIADVVSRWTGIPVSRLMEGETEKLLHMEERLHDRLIGQ
                     DEAVIAISDAIRRARAGLKDPKRPIGSFIFLGPTGVGKTELARALAEFLFDDEGAMVR
                     IDMSEYMEKFAVSRLVGAPPGYVGYEEGGQLTEAVRRRPYQVVLFDEIEKAHSDVFNI
                     LLQLLDDGRLTDSQGRTVSFRNTVVIMTSNIGSAGLIEAAESGADAFARAAEEVKASL
                     RDHFRPEFLNRIDEIIVFKPLAEDQLKEIVGLLLGGVERRLAESRISLEVTDAARALV
                     AREGYDPQYGARPLRRAIQRLIENPLARQLLAAEFTAGDTIRIDEREGSLVFEKVSST
                     PEQPEPKRPEPVTA"
BASE COUNT          664 a         1543 c         1482 g          765 t
ORIGIN      
        1 gtcggccggg agcgggtggt gatgctacga gacccgtgcg acctgccgat catcgggccc
       61 ctgctcaacc tcgttccgct ggcgcggaac ttctcgcgga gcgaccatcg gcgcttctgg
      121 gacgcgcggc tgccggcgat ccaggtcacg aacaccgcca acttccgcaa tccgaactac
      181 caccgggccg gcgacaagcc ggcgaccctg gattacgaga cgctcgccga catcacggtc
      241 gccacggtgc tcgccgttcg ccgtctcgcg ggctgagtca gcgccgctgg ctggctgcta
      301 gactccgcgg ccgatggaca agcttttcgc gccgttcaac ccgaccaact tcggcgacct
      361 cttcaccatg atgtgggtcg gggcattcgt gatcctggcc ggggcggtca tcgtctacaa
      421 cacggcgcag cgcatgtacc ggcgctaccc ggccatcctg gcgctccacg aatggatctt
      481 ctgggccatc ttcggcacct ggctggtggt gccgctgctg gtggtcatcg gcgttccgct
      541 ggcacttctg ctcctgctga tcgtgccggg gatcgcgatt gccacctacg ccgcattcgt
      601 gaagttcccg ccgatcatcg aggccgccaa cgtcgagatc cggcgccgcc ggttcgtccc
      661 gccaccgcgc cgcgacgtgc gggagcgccc gaagccgacc cccgcgggac gccgtcgccg
      721 ccaccgctag gcgctggccg gcttcgtcgc gcggatgatg gccgagtgca tcccgtcggc
      781 aactgagtgc gttggcgtga cggagacatc ggtcaggcca acctcgcgga ggcccgcctc
      841 gaactctccg aacgagagcg caccggcgat gcagcccacg taggatccac gctcggcgcg
      901 ctgctccgcc gtcagcatgt cgtcggcgac gatgtcgctg atgccgatgc gcccgcccgg
      961 ccgcagcacg cgcgcgatct cgcgaaagac cgacggcttg tcggccgcca ggttaacgac
     1021 gcagttgctg atcacgacgt cgatgctctc ggcggggagc gggattgcct cgatctgacc
     1081 cttcaggaat tccacgttgc ccgcgcctgc ctccgcggcg ttgcgctggg cgagggtcag
     1141 catctcatcg gtcatgtcca ggccgaaggc tcgtccggtg ggaccgaccc gccttgcgga
     1201 gagaaggacg tcgatgccgc cgcctgatcc caggtcgagg acacgctcgc cgggatgaag
     1261 gtccgccacc gcgatggggt tgccgcagcc gagcgaggcc atcacggccg cgctgggcag
     1321 ctcctcccgc tcgagtgcgg agtacagatc gggcccgacg ctcgtgtccg tgcagcaggc
     1381 atcgccacag cagctggtgt tctcgccgcg ggcaagcacc tggagcgctg actgggcgta
     1441 gcgctcgcgc acggtctcgt gaatctggtc ggtcatcggg ttctcctccg gatgcgtcta
     1501 cccgacggtg ctcggcgtca gctgctctct ccgtcccgcg gaaagcccag cttttcagcc
     1561 tcgtggaact cgtcgccggc ggactcctcg gcgtcctcgg cctgttcgag gtcctggttc
     1621 gctcggacct ggtcaccttc agcggacgcg atgaggatac gtgccgccgt ggcagcgcgc
     1681 tcggcagctc tcttggcgct ggcggcagcc ttgagcgctt gatccaccgc agacgcagcc
     1741 gccctcgcag cggcttccgt ctcagatgcc gcttcatcgg ctgccatggc ggcctgcaac
     1801 gcgaggttgg cgaccttcga tgcagccttt gcggcggcgg tgtctcgtcc agccgatcgc
     1861 caatcggcta gcagctcgga cgcggtgttg ggctgcttct tgggcattgc gacactccgc
     1921 tcacgttggg acgatggagt gtaatcggtc cctgatcggt gcaatgtggt ccttgcggct
     1981 cagactctca ggccgtgccg cgcttgtccc gtctgtcgcg ccccgcgatc tgatgcgtta
     2041 ccgactgatc cgaaggtgat ggcggcaagg aagatctcct tgtcgcgcgg aacgcgcccg
     2101 aacggcagga ggctggccca gccgaggctc atcgtcagca gcttgccggt gaagcggccg
     2161 accagtccga agagggttgc caagattgcc gtctcagatc cagtgcctga accgggcaag
     2221 gcccaggatc agcaccgcga gaccgagcat gcccgccagc accagccaga agtggctggg
     2281 atcatcgaag aacccgagct tgaagttcat ccccatcacg ccggccagga cgacggccgg
     2341 cagcaggaca gccgacagga tggtgagcgc cttcatgacg tcgcctgagc gctgcgccat
     2401 gcggccgagg tagatgtcag aggagccgac cagcagctcg cgggtgcgtt cgacgccgga
     2461 gatcgcctgc tcgagccgat ccaggagtcc cggccacggc tttccgaggt cttcgtgcag
     2521 cgcgaagtcg ggacgggaca ggggaccgaa cgtctcgcgc tgggggacca ggatgcgtcg
     2581 cagtttcgcc gcgcggcgtc ggagcgccac cacccgctcg aggaagtcgg cccgatcctc
     2641 gccgcgcagc gcgatctcgt cgagccggtc cacctgttct tcgatctcct ccacctgccg
     2701 gcgaaaggcg ccgagcatcg tatccaccag gccaccgacg agcgagccgg catcgaggct
     2761 gcccagccgc tgctcgtcgc tgacgtgcga ttcgaactcg tcgagcgccg cgaccgggct
     2821 cgcatgaagc gtcacgaccg cgttgcgtcc agccaccagg tcgaccgcca ccgggaagtc
     2881 tcgtccgtct cgatcctcga ttgagaggac cgtgagcagg atcgccgcgg gtgcccgaac
     2941 aagccgggcc tcatcgcccg gggtggcgag ggcggtcatg atcggggcgg gaaagcggaa
     3001 ggcagcctcg atctgctcga gtgccgcctg atcgggcgcg tcgatgtcaa cccagagcaa
     3061 cgtctcgtcg gtcagctcgg ccacggccgt cgcgctcagc tcgatctctt tgtcttcgcc
     3121 tcgtgcgtcg tacaggcgcg aacggatccc cattcgcgga aggttcggca caaccgggtg
     3181 tcaaccgtgg ccgatggcgg ccggccagca ttgcaggtgg cttgcggatg attacgccgt
     3241 gacgggctcc ggcctcttgg gctccggctg ctcgggcgtc gaggagacct tctcgaagac
     3301 gagggaaccc tcgcgctcgt cgatccgaat cgtgtcgccc gccgtgaact cggcggcgag
     3361 aagctgcctg gccagcgggt tctcgatgag gcgctggatg gcccgccgca gcggcctggc
     3421 gccgtactgc gggtcataac cctcacgcgc gacgagggcg cgggcggcat cggtcacctc
     3481 cagggagatg cgcgactccg ccagccggcg ctcgacgccg ccaagcagca ggccgacgat
     3541 ctccttcagc tggtcctcag ccaacggctt gaagacgatg atctcgtcga tccggttgag
     3601 gaactcgggc cggaagtggt cgcgcaggct ggccttgacc tcctcggcgg ccctggcgaa
     3661 ggcatcggct cctgactcgg cagcctcgat cagccccgcc gagccgatgt tgctggtcat
     3721 gatcacgacc gtgttgcgga agctgacggt tctgccctgg gagtcggtca ggcgtccgtc
     3781 gtcgaggagc tgcagcagga tgttgaagac gtccgaatgg gccttctcga tctcgtcgaa
     3841 caggacgacc tggtacggcc gtcgccgcac cgcctcggtg agctggccgc cctcctcgta
     3901 gccgacgtag cccggcggag cgccgaccag ccgcgacacg gcgaacttct ccatgtactc
     3961 gctcatgtcg atccgcacca tggcgccctc gtcgtcgaac aggaactcgg ccagcgcgcg
     4021 ggcaagctcg gtcttgccga ccccggtggg gcccaggaag atgaatgagc cgatcggccg
     4081 cttcgggtcc ttgagcccgg cgcgggctcg acggatcgcg tcgctgatgg cgatcaccgc
     4141 ctcgtcctgg ccgatcagcc ggtcatgcag ccgctcctcc atgtgcagca gcttctcggt
     4201 ctcgccctcc atcaggcggc tgaccgggat gccggtccat cggctcacca catcggcgat
     4261 gtcgtccgcg tcgacctcct ccttgagcat cagcgagccc gatgccttca gctccttgag
     4321 ccttccctcc tgctcggcaa gctgcgtggc gagctcgttg gcgcgcccgt acttgagctc
     4381 ggctgccctg gcgtagtccg cggcgcgctc ggctgcctcg atctcggggg tgagcttttc
     4441 gagctgctcg cgcg
//
LOCUS       DATCQR010000104         7553 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_83227, whole genome shotgun sequence.
ACCESSION   DATCQR010000104 DATCQR010000000
VERSION     DATCQR010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 7553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7553
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83227"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(98..1714)
                     /gene="murJ"
                     /locus_tag="VIN32_03505"
     CDS             complement(98..1714)
                     /gene="murJ"
                     /locus_tag="VIN32_03505"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEY8921680.1"
                     /translation="MSDAVGVETPPEATGPSVGRVLANASLILTVAALASRLLGWIRL
                     LVIGSQFGASADLDAYFAAFRIPDAIFQLVVAGALSAALIPVFSSYRAREDEREAWRL
                     ASSVINLVLIALAVFSLVMAILAPWVVPIIAPGFDAPTTELTVRLTRLMLLSPVFIGM
                     GAVVSGLLNSYGRFGVPALAPLVYNLAIIVAAVVLAPTLGVEGLAIGVVAGSILHLAI
                     QLPQLRSVGRHYDLTIGLGHPGVRKVAWLMGPRLLGLAAGQVNFVASTILASNLGVGA
                     VTAYNYAFQLSQIPVGVLGVSVAVALFPTFSRDAALGRIAEIRRQLSTSLRILIFLAA
                     PLTAVMIVLARPIAAVFFQYGLFTDAAAERTAGALVFFSIGLTGHIVVHVLTRAFYAM
                     QDTRIPVLWAIFAVAINVPLMVILSGPMGVEGLALALSISATLEVIGLALALRRRIDS
                     IDEGTILQSALRSALAAVVAGAVMLGGLAAAQEWFGPMLANGAGRVLVLLVLVAAGTA
                     VYLVAGILMHSEELGQVRRYLFHRRSAAAA"
     gene            complement(1711..2697)
                     /locus_tag="VIN32_03510"
     CDS             complement(1711..2697)
                     /locus_tag="VIN32_03510"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HEY8921681.1"
                     /translation="MAEAAALSGDAAAALQRFLGHLSSRNSSPGTIREYRRHVAQFFA
                     FLGSRGVDWKSPDRATVRAYLGTLAERGLSASTVGGQLAAIRSLYRHAARQGWIAGDP
                     VAGVRSPKRPGRLPRVLSVDGAARLVEAPANFMRVGRRRRDPAVADATARRDAAVLEL
                     LYATGMRISELASLTVDRLDPTRRRLRVIGKGSKERELLFGEPAARAVSAYLASGRPT
                     LAARSTRPVAALFLNSAGGPLTGRGARMIVDRWVTASGAPASTSPHTLRHSFATHLLE
                     GGADLRTVQELLGHASLATTQIYTHLSDASLRTAYRSAHPRSGRTGSARPTE"
     gene            complement(2690..4885)
                     /gene="topA"
                     /locus_tag="VIN32_03515"
     CDS             complement(2690..4885)
                     /gene="topA"
                     /locus_tag="VIN32_03515"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012191913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEY8921682.1"
                     /translation="MPPKKRSSGNLVIVESPAKAKTIERYLGPGYTVTASYGHVRDLP
                     QKNLGVDLEHDFAPRYEPLKERRKELEKLADQARKAETVWLATDLDREGESIAWHIAD
                     YAGLDDAHVRRVTFSEITKPAIEAAFAHPRAVNLDLVNAQQARRVIDRLVGYKLSPLV
                     SSKIRRGLSAGRVQSVAVRLVVDREREIDAFTAEEYWTIAAELTRRGESGKFTAELTK
                     IDGKKARIGSEGDAREHETALRAAEYKVASVSRTQQKKSAPPPFTTSTLQQEASRKLS
                     FGARRTMSVAQSLYEGVSLPEGQVGLITYMRTDSLSIASGAVSEARGVIGQRFGADFV
                     PDKPNAFRNRSRGAQEAHEAIRPSSFARTPESMRAHLKPDEYRLYELIWRRAIASQMA
                     PARFDQVGVDVNAGIYTLHAGARKRVFDGYQAVYVEGKDDEEDERLAVLPDLRDDEPL
                     DLAALAAEQHFTQPPPRFTEATLIKALEEHGIGRPSTYAPTIETIRARGYVTIKERRL
                     FPEESAFKVTDLLVEHFPEVVDIEFTARMEEQLDEVAAGTNEWVPMVRAFWDPFSVQI
                     AEGRANIAKQVELTDIVCPLSGDLLVKRFGRNGWFLGCSGYPECKYTAPLPGEEAEAA
                     ALPGVGEECPECGKGHLVARTGRFGPFVGCDRYPDCKYIKRETPAAERFGVCPKCGQG
                     TVVTKRARRGKRPFWGCDRYPDCDYVSWTRPGRPEEEGEAPAAAAAASG"
     gene            complement(4932..6548)
                     /locus_tag="VIN32_03520"
     CDS             complement(4932..6548)
                     /locus_tag="VIN32_03520"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEY8921683.1"
                     /translation="MISLSTARFGNRAPFASLSYQLRRHRAITRILIATLAISLAAAV
                     STGGAAQAGLTTVLPAPASILPVGIGVGIGTGQSVTLAFTHSMDVASVDSALSIAPAH
                     PVRIIWSDDGRQLHLIPDGLWSPDARYALVVAGTARTAAGALLGTPARFSFTTQTAPR
                     VTDFSLHYVAETTPPAVALRATETDQLGPPTDTTSDVSAATSIQIAFTASMNQAEVER
                     AFVLSPGAPGAFRWDGRTLTFTPIERLVAGARYAVSLVGVHDRAGNPLGGDASFSFTT
                     RPGAQLVQSTPRADSTWVTAGEIGLWFSQPVDRAAINAALRVTDHATAARLTGKTSWN
                     AAGTQLAFTPDRALAAGHRFDVRLAGGAVDADGNAVTAALTFSTMPPLSGAPPRTRTR
                     VAVPAPAPSSSLVGYALNQVNAARAAYGLRPLVLSSAISAVSNAHAWDMLRNNYFSHT
                     SLDGRTKQDRLRNAGISFSWSGENICYSNNSARSPSAVLNWCHSQFMSEPYPGYSNHI
                     GNILGTHYTRIGIGIAVSGSKVYVVWDFTD"
     gene            complement(6632..>7553)
                     /gene="dprA"
                     /locus_tag="VIN32_03525"
     CDS             complement(6632..>7553)
                     /gene="dprA"
                     /locus_tag="VIN32_03525"
                     /inference="COORDINATES: protein motif:HMM:NF014533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HEY8921684.1"
                     /translation="VATATRRCGGLIRTALDPEYPAALSAADPRPPVLYLAGDPASLT
                     RPCVAIVGTRRASGYGRSCAAEIAEELARAGVTVVSGLAIGIDAEAHLAAITAGGVSV
                     AVLPSPLDRIYPPRHRDLAARLVATGGALISELAPGHAPGKPDFARRNRIIAGLARAV
                     VVVEAPDRSGALLTAAAAIDHGREVFAVPGPIDAIASRGCNRLIADHLATIVTSAGAL
                     LHRIGAESRQGPVSVASLSDSEAMVLHALLQRPGSIEELIGRTDLSTGSLAATLTLLE
                     ARGLVESYGGATFHPTLTARRIGRPVGLDS"
BASE COUNT         1176 a         2567 c         2730 g         1080 t
ORIGIN      
        1 gcccaggccc aatcgtgcag gaaatcgccg gccgggctcc ggcccaggag cgactgccag
       61 gcgcgctcat cccccgccgt taccggtcga aaatggctca tgcggccgcc gccgaccgcc
      121 gatggaagag gtagcgccgc acctgcccca gctcctcgga atgcatcagg atccctgcca
      181 ccaggtagac cgccgtgccg gctgcgacca gcacgagcag caccagcacg cgtccggcgc
      241 cattcgccaa catcgggccg aaccattcct gggcggcggc cagcccgcca agcatgacgg
      301 cgccggcgac caccgccgcc agcgcagagc gaagggccga ttgcaggatc gtcccctcat
      361 cgatcgagtc gatccgtcgg cgcagcgcaa gggcaaggcc gatcacctcg agcgtggcgc
      421 tgatcgagag ggccagcgcc aacccctcga cccccatcgg tcctgacagg atgaccatca
      481 gcggcacgtt gatggccacc gcgaagatcg cccacaggac ggggatgcgg gtgtcctgca
      541 tggcatagaa ggcacgcgtc agcacgtgca ccacgatatg gccggtcagg ccgatgctga
      601 agaagacgag tgctcccgcc gtgcgctccg cggccgcatc ggtgaagagt ccgtactgga
      661 agaagaccgc tgcgatcggg cgcgccagca cgatcatgac cgccgtgagc ggcgcggcca
      721 ggaagatgag gatgcgcagg ctggtagaca gctgtcgccg gatctcggcg atgcgcccca
      781 gcgcagcatc gcgcgagaag gtcggaaaga gggcgaccgc cacgctcact ccaagcaccc
      841 caaccgggat ctggctcagc tggaatgcgt agttgtacgc ggtcaccgcc cccacgccca
      901 ggttgctggc aaggatggtg ctggcgacga agttcacctg tccagccgcc aggccgagca
      961 ggcgcggacc catcagccag gctaccttgc ggacccccgg atgcccaagg ccgatggtca
     1021 ggtcgtagtg acgccccacg ctccgcagct gtggcagctg gatcgccagg tgcaggatcg
     1081 agccggccac cacgccgatc gccaggccct cgaccccgag cgtcggcgcc aggaccaccg
     1141 ccgccacgat gatcgccagg ttgtagacca ggggcgcgag ggccggcacg ccgaagcgcc
     1201 cgtagctgtt cagcagcccg ctgaccaccg cgcccatccc gatgaagacg gggctgagca
     1261 gcatcagccg cgtgaggcgc accgtcagct cggtggtcgg cgcgtcgaag cccggcgcga
     1321 tgatcggcac cacccatggc gccaggatcg ccatgacgag gctgaagacg gccagcgcga
     1381 tcaggaccag gttgatgacg ctgcttgcca ggcgccaggc ctcgcgctcg tcctcccggg
     1441 cgcggtagct gctgaagacg gggatcaggg ctgcggagag cgccccggcc accaccagct
     1501 ggaagatggc gtcggggatg cggaaggcgg cgaaataggc gtcgaggtcc gccgaggcgc
     1561 cgaactgcga gccgatgacc agcagccgga tccagcccag cagccggctg gcgagcgccg
     1621 ccacggtcag gatcaggctg gcgttggcca gcacccgacc gaccgatgga cccgtcgcct
     1681 ccggcggcgt ctccacgccg acggcgtcgc tcactctgtg gggcgggcgg atccggtccg
     1741 gcccgatcga gggtgcgcgg agcgataggc cgtgcgcagc gacgcgtcgg agaggtgcgt
     1801 gtagatctgg gtggtggcca ggctggcatg gccaagcagc tcctgcaccg tgcgcaggtc
     1861 agccccgccc tcgagcaggt gcgtggcaaa cgaatggcgc agcgtgtgcg gcgaggtgct
     1921 ggccggagca ccggatgcgg tcacccagcg gtccacgatc atgcgagccc ctcgtccggt
     1981 cagcggcccg ccggcgctgt tcaggaagag cgccgccacc ggtcgcgtgc tgcgggcggc
     2041 gagggtcggc cgtcccgagg ccaggtatgc gctcaccgcg cgggcagccg gctcgccgaa
     2101 gagcagctcg cgctccttgc tccccttgcc gatcacccgc agccggcgcc gagttggatc
     2161 gagacggtcg accgtgagcg aggcgagctc gctgatgcgc attccggtcg cgtacagcag
     2221 ctcgaggacc gcggcgtcgc ggcgtgccgt ggcatcggcc acggcggggt cacgcctgcg
     2281 ccgccccacg cgcatgaagt ttgccggcgc ttccacgagg cgggccgccc cgtccaccga
     2341 aaggacccgt ggcaggcgtc ccgggcgctt gggcgagcga acccccgcca ccgggtcgcc
     2401 ggcgatccag ccctggcggg ccgcgtgtcg atacagcgag cggatggccg ccagctgccc
     2461 cccaacggtc gaggcggaca ggccgcgttc cgcaagcgtg cccaggtagg cgcgcaccgt
     2521 ggcgcggtcg ggggacttcc agtcgacccc gcgcgagccg aggaaggcga agaactgcgc
     2581 cacgtgccga cggtactcgc ggatcgtgcc cggcgaggag ttgcggctcg agagatgtcc
     2641 gaggaagcgc tgcagggcag ccgcggcgtc gccgctcagc gcggccgcct cagccacttg
     2701 cggccgcggc tgccgcggga gcttcgccct cctcttccgg gcgacccggc cgggtccagc
     2761 tcacgtagtc gcaatcggga tagcggtcgc agccccagaa cggccgcttg ccgcggcgag
     2821 cgcgcttcgt gaccaccgtc ccctgcccgc acttcggaca gacgccgaag cgctcggcgg
     2881 cgggcgtctc gcgcttgatg tacttgcaat cgggatagcg gtcgcagccc acgaacggcc
     2941 cgaatcggcc tgtgcgcgcc accaggtggc ccttcccgca ttcggggcac tcctcgccga
     3001 cgccgggcag cgcggccgcc tccgcctcct cgccgggcag cggcgcggtg tacttgcact
     3061 cggggtagcc gctgcagccc aggaaccagc cgttgcgacc gaagcgcttg accagcaggt
     3121 cgccgctcag cgggcagaca atgtcggtca gctcgacctg cttggcgatg ttggcgcgcc
     3181 cttcggcgat ctggacgctg aagggatccc agaaggcgcg caccatcggc acccactcgt
     3241 tcgtccccgc agcaacctcg tcgagctgct cctccatccg ggccgtgaac tcgatgtcca
     3301 ccacctctgg gaagtgctcc accaacaggt cggtgacctt gaaggccgac tcctcgggga
     3361 agagccgccg ctccttgatg gtgacgtagc cgcgggcgcg gatggtctcg atggtggggg
     3421 cataggtcga aggccggccg atgccgtgct cctcgagcgc cttgatgagt gtcgcctcgg
     3481 tgaagcgcgg cggcggctga gtgaagtgct gctcggcggc cagcgcggcg aggtcgagcg
     3541 gttcgtcgtc gcgcaggtcg ggcaggaccg ccaggcgctc gtcctcttca tcgtccttcc
     3601 cctcaacgta gacggcctgg tagccgtcga agacgcgctt gcgtgccccg gcgtgcagcg
     3661 tatagatgcc cgcgttgacg tcgacgccga cctggtcgaa ccgggctggg gccatctggc
     3721 tcgcaatcgc gcgcctccag atcagctcgt agagtcggta ctcgtcgggc ttgaggtgcg
     3781 cccgcatgct ctccggcgtc cgggcgaagc tcgacgggcg aatcgcctcg tgggcctcct
     3841 gggcgccacg gctccggttg cggaacgcgt tcggcttgtc cggcacgaaa tcggcgccga
     3901 atcgctggcc gatgacgccg cgcgcctcgc tcaccgcgcc actcgcgatc gagagcgagt
     3961 cggtgcgcat gtacgtgatc aggcccacct ggccctcggg cagggagacg ccctcgtaca
     4021 gcgactgggc gacgctcatg gtgcgtcgtg ccccaaagct cagcttgcgg gatgcctcct
     4081 gctgcagggt gctggtcgtg aatggtggcg gcgccgactt cttctgctgc gtacggctga
     4141 ccgatgcaac cttgtattcg gcggcgcgca atgcggtctc gtgctctcgc gcgtcgccct
     4201 cggagccgat gcgtgccttc ttgccgtcga tcttggtgag ctcggcggtg aacttgccgc
     4261 tctctccgcg acgggtcagc tcggcggcga tggtccagta ctcctcagcg gtgaaggcgt
     4321 cgatctcgcg ctcgcggtcg acgaccaggc gcaccgccac gctctgcacg cggccggcgg
     4381 agaggccgcg ccggatcttg ctgctgacca gcgggctgag cttgtagccc accagccgat
     4441 cgatgacgcg ccgcgcttgc tgggcgttga ccaggtcgag gttgacggcg cgcgggtggg
     4501 cgaaggccgc ctcgatggcc ggcttggtga tctcgctgaa ggtcacgcgg cgaacgtggg
     4561 catcgtccag tccggcgtag tcggcgatgt gccaggcgat cgactcgccc tcgcgatcga
     4621 ggtcggtggc gagccatacc gtctcggcct tgcgtgcctg gtcggcgagc ttctcgagct
     4681 ccttcctgcg ctccttgagc ggctcgtagc gcggggcgaa gtcatgctcg agatcgaccc
     4741 ccaggttctt ctggggaagg tcgcggacat ggccgtagga ggcggtgacg gtgtagccgg
     4801 ggccaaggta ccgctcgatc gtctttgctt tggcgggcga ctcgacgatc accaggttgc
     4861 cgctggaacg cttcttcggg ggcatcggtc agggagtgta gcacccgtca tcgggcgctc
     4921 cggagcacgg actagtccgt gaaatcccac acgacgtaga ccttggagcc actgaccgcg
     4981 atcccgatgc cgatccgggt gtagtgcgtt ccgaggatgt tgccgatgtg gttggagtac
     5041 cccgggtatg gctcggacat gaattgcgag tggcaccagt tgagcacggc gctcggcgac
     5101 cgcgccgagt tgttgctgta gcagatgttc tcgcccgacc agctgaacga gatgcccgcg
     5161 ttgcgcagac ggtcctgctt ggtcctcccg tcgaggctgg tgtgactgaa gtagttgttg
     5221 cgcagcatgt cccacgcgtg agcgttggag acggccgaga tcgccgagga gaggacaagc
     5281 ggcctcagac cgtaggcggc acgggcggcg ttgacctggt tcagcgcgta gccgacaagg
     5341 ctcgacgacg gagccggcgc tgggaccgcg acacgggtcc gcgtgcgcgg gggcgcgcct
     5401 gacagcggcg gcatggtact gaaggtcagg gcggcggtca ccgcattgcc gtccgcgtcg
     5461 accgctccgc cggccaggcg cacatcgaag cgatggcctg cggcgagggc gcggtccggt
     5521 gtgaaggcca gctgcgtgcc ggcggcattc cacgacgtct tgccggtcag gcgggcagcc
     5581 gtggcgtgat cggtgacccg aagtgcggcg ttgatcgctg cgcgatccac cggctggctg
     5641 aaccagaggc cgatctcccc ggcggtcacc caggtcgaat cggcccttgg cgtgctctgc
     5701 accagctggg caccgggccg tgtcgtgaac gagaacgagg catcgccgcc cagcgggttg
     5761 ccggcgcggt catgcacacc caccagggag acggcatacc gggcgcccgc gacgagccgc
     5821 tcgatcggcg tgaaggtcag ggtcctgccg tcccagcgga aggcgccggg tgctccgggg
     5881 ctgagcacga aggcacgctc cacctcggcc tggttcatcg acgcggtgaa ggcgatctgg
     5941 atcgaggtcg cggcactcac gtccgaggtg gtgtcggtcg gcggcccgag ctgatcggtc
     6001 tcggtggcgc gcagcgcgac ggcgggtggt gtcgtctccg ccacgtaatg caggctgaag
     6061 tcggtgacgc gcggcgccgt ctgggtcgtg aagctgaagc gggccggcgt tcccagcagg
     6121 gcgcccgcgg cggtgcgagc cgtcccggcc acgaccaggg cgtaacgggc atcaggcgac
     6181 cagaggccat cgggaatcag gtggagctgg cgcccgtcgt cagaccagat gatgcgcacc
     6241 gggtgcgccg gggcgatcga aagcgccgag tccactgacg cgacatccat cgaatgggtg
     6301 aaggccagtg tcacggactg tccggtgccg atgccgacgc cgatccccac cggcaggatg
     6361 gaggctggtg ccgggaggac cgtcgtcagc cctgcctgcg cggcaccgcc cgtagatacg
     6421 gcggcggcga gggagatggc cagggtggcg atcaggatgc gggtgatggc acgatggcgt
     6481 cgcaactggt acgagagcga cgcgaatggg gcgcgatttc cgaagcgcgc agtactcagc
     6541 gagatcaata gacagtgact cctgtcacgg ggcggaccgt cgtcgggcac gacccacggc
     6601 tgccttgtgg cgcgcgaagt atacccaccg atcagctgtc aagcccaacg gggcgcccga
     6661 tgcggcgcgc ggtgagggtc gggtggaagg tcgcgccacc gtaggattcg accaggccgc
     6721 gagcttccag cagggtcaag gtggccgcca gcgacccggt cgacaggtcc gtccgcccga
     6781 tcagctcctc gatcgagccg gggcgctgca gcagggcgtg gagcaccatc gcctcggaat
     6841 cggagaggct ggcgacgctc accgggcctt ggcgagactc cgcgccgatg cgatgcagca
     6901 gggcacccgc cgacgtgacg atcgtcgcca ggtggtccgc gatgagccgg ttgcacccgc
     6961 gcgacgcgat ggcgtcgatc ggaccgggga ccgcgaacac ctcccgaccg tgatcgatcg
     7021 ccgcggcggc ggtgagcagc gccccggagc gatcgggcgc ctcgaccacc accaccgcgc
     7081 gcgccagccc ggcgatgatc cggttgcgcc gcgcaaagtc cggcttgccc ggagcatgac
     7141 cgggcgcaag ctcggagatg agcgccccgc ccgtcgccac caggcgagcg gccagatctc
     7201 gatgacgcgg tgggtagatg cggtcgagtg gcgacggcag gactgcgacg ctcacgccgc
     7261 ccgctgtaat ggcggccagg tgagcctccg cgtcgatccc gatcgcgagc ccactgacga
     7321 ccgtgacgcc agcgcgcgcc agctcctccg cgatctcagc cgcgcacgaa cgcccgtagc
     7381 cgctggcgcg gcgggtgcca acgatggcga cgcacggccg cgtcaacgag gcgggatcgc
     7441 ccgccaggta caggaccggc gggcgcggat cggccgcgct gagggcggct ggatactcgg
     7501 gatcgagggc ggtgcggatg agtccgccgc atcgccgcgt cgcggtcgcg acc
//
LOCUS       DATCQR010000105         3178 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_114481, whole genome shotgun sequence.
ACCESSION   DATCQR010000105 DATCQR010000000
VERSION     DATCQR010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3178
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114481"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(528..926)
                     /locus_tag="VIN32_03530"
     CDS             complement(528..926)
                     /locus_tag="VIN32_03530"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HEY8921685.1"
                     /translation="MSQDSGRLRRPPAERFAGSEHVFNLPTVAGKLWREDHPAADGHR
                     QMTLFHAGDLALVLFDFEAGGVLADHEADGYVTIHTVAGELEVSTSEATHRLPAGSLL
                     VLAPGVRHDVTARVASQMLLTVHLVKPTGP"
     gene            complement(995..>3178)
                     /locus_tag="VIN32_03535"
     CDS             complement(995..>3178)
                     /locus_tag="VIN32_03535"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEY8921686.1"
                     /translation="ICRCGAYARILAGLRHAAQGEARPQAAATSPPDVETPDGQLEHA
                     RASPWDLARDDEALFDRLGDGLLLVPDDEGRAWLHVGADGRNTVASGKVEMGQGSTAE
                     LIRLASEELCAPASSVRALLGDTDFTPYDEGTYGSLTTPEVVPDVRATAATAREALLR
                     IAADGWGTDVAALTAADACVTSVDGTRSASYAELVSEMRRIEPVASEAALTAPDHWRL
                     AGTRTSVTDGTDAVTGHRMYTTDLSLPDMLHGKILRPPAFGATLRSVDTSAAAAIPGV
                     SVVRDGDFIGVVAADPLVAEDAVTKIRAEWDPVSQPGNADLVSHLRAHPITVEGWEGG
                     FRDKAGHVDRALASAPVRLDATYTTAYLAHQPLETRAALAHWAGGRLTVWTGTQRPFG
                     VREELAEAFGIGEEMVRVIVPPTGSAFGGKHTGECSLEAARLARAAGRPVKVRWTREE
                     ETTWGYFRPAAVIDIRSGARTDGTLTAWEFTNLNSGARAIEPPYRVSNRRITYQPADS
                     PLPQGSYRALSATANTFARESHLDEMAHRVGVDPVEWRLRHLTDDRLKAVLQAAADRA
                     GWTRDDHADASGQGMGIACSMEKDSRVATCAEVRAAPGDPLEILRIVTAFDCGAILDP
                     DNLANQVEGALVMGLGGALFEAIHFADGRVTNAAQLQYPVPRFHDLPPIEVVLLNRPD
                     LPSAGAGETPIVALAPAIANAIFAASGVRLRSLPLVPDGRVPNPA"
BASE COUNT          479 a         1122 c         1105 g          472 t
ORIGIN      
        1 gtgacttgca gagtggtcat gccgcgtcct ccgtgtcgaa ccgtcgcatc ccaatgagcg
       61 cggacgtgtc tcgggtcatc atgcacccgc gtggcaagcc tgccgctcgg gtcgcatcgt
      121 cgtcactcct tgggtcgcgg actggctgct cgacatgcca tgcgcccgac gcggacgacg
      181 cccgcggagc gcacgcgccg gatcaaagct tcgtgcgcag actcggagct attgacaggt
      241 ggagctgtgt gaaatctggc gggctgaggg tcgtcacggg gggtcaatgg tagccgggcc
      301 tgactcatcg cggcctccct tcacgcaggc tgtgggccgg gttggggccc ggcaatgggc
      361 ggccggcgga gctggggctc taaccacgac ccctggcgaa cattgagggc tacttgcgcg
      421 ccttcactca ccagcacttg tagcggggcc ggcgctcaat tacgcgggac ctactagccc
      481 gaccgcgcac ctcgcgcgga tgagtctcag cgccgcggac acaagcgtca cggcccggtc
      541 ggcttgacga ggtggaccgt cagcaacatc tgactggcga cccgggcggt gacatcgtgc
      601 cggacacccg gggcgaggac gaggagcgaa cccgccggta gccggtgcgt cgcctcggag
      661 gtcgagacct cgagttcgcc agccacggta tggatggtta cgtacccatc ggcctcgtga
      721 tcggcgagca cgccgccagc ctcgaagtcg aacagcacga gcgccaggtc gcccgcatgg
      781 aacagcgtca tctgccgatg gccatccgcg gccgggtgat cctcgcgcca caacttgcct
      841 gccaccgtgg gcaggttgaa gacgtgctcc gagccggcga atcgctcggc cggtgggcgg
      901 cgcaaccgac ccgagtcttg cgacatggca tctcctccgg tcctgatggc actgaccgta
      961 caccagcgca cacctacctc gcgcggaccg atgctcaggc cggattcggc acgcgtccgt
     1021 ccgggacgag cggcagcgac cggagccgaa ccccggatgc cgcgaaaatc gcgttggcga
     1081 tggccggcgc cagggccacg atgggcgtct cgcccgcgcc ggccgagggg aggtctggcc
     1141 ggttcagcag caccacctcg atcggcggga ggtcatggaa gcgcggcacc gggtactgca
     1201 actgcgcggc gttggtgacc cggccatcgg cgaagtgaat ggcctcgaag agcgcgccgc
     1261 ccagccccat gaccagcgcg ccctcgacct ggttggcaag gttgtccgga tccaggatgg
     1321 cgccgcagtc gaaggcggtc acgatccgga ggatctcgag cgggtcgccg ggcgccgcgc
     1381 gcacctcggc gcacgtcgcc acgcgcgagt ccttctccat gctgcacgcg atccccatgc
     1441 cctgaccaga ggcatcggcg tggtcatcac gcgtccatcc ggcccgatcg gcggccgcct
     1501 gcagcaccgc cttcaagcgg tcatcggtca ggtggcgcag ccgccactcg acggggtcga
     1561 caccaacccg atgcgccatc tcgtccaagt gggattcccg ggcaaacgtg ttcgcggtcg
     1621 cggagagggc acgatacgag ccttgcggaa gcggagaatc cgccggctgg tacgtgatgc
     1681 gacggttcga aacacggtag gggggctcga tcgcgcgggc gccggagttc agattggtga
     1741 actcccaggc cgttagcgtc ccgtccgtcc gagcgccgct gcggatgtcg atgaccgccg
     1801 ccggccggaa gtagccccac gtcgtctcct cctcccgggt ccagcgcacc ttcaccgggc
     1861 gtccagcggc gcgggccagg cgcgcggcct caagactgca ttccccggtg tgcttgccgc
     1921 cgaaggcgct gcccgttggc ggcacgatca cccgcaccat ctcctcgccg atgccgaagg
     1981 cctctgccag ctcctcgcgc acgccaaacg gacgctgggt cccggtccag accgtcagcc
     2041 ggccaccggc ccagtgcgcc agcgcggcgc gagtctcgag gggctggtgc gcgaggtagg
     2101 cggtggtgta ggtggcgtca agccggacgg gagccgatgc cagcgcgcga tccacgtggc
     2161 cggccttgtc ccggaaacca ccctcccacc cctccacggt gatcggatgg gcgcgcaggt
     2221 ggctgacgag gtccgcgttg cccggctggc tgacagggtc ccactccgca cggattttgg
     2281 tcaccgcgtc ttccgcgacg agcgggtcgg ctgcgacgac gccgatgaag tcaccgtcgc
     2341 ggaccacact cacgcccggg atcgcggcgg ctgccgacgt gtccacggag cggagcgtgg
     2401 ctccgaacgc gggcggtcga aggatcttcc cgtggagcat gtctggcagg gagaggtcgg
     2461 tggtgtacat ccggtgcccg gtgactgcgt ccgtcccgtc agtgacggat gtccgcgtcc
     2521 cagccaaccg ccagtggtcg ggtgccgtca gcgctgcttc ggaagccacc ggttcgatgc
     2581 gtcgcatctc gctcaccagc tcagcgtagg aggccgatcg ggtgccgtcg accgaggtca
     2641 cacaggcatc ggccgccgtc agggccgcga catcggtccc ccacccatcc gccgcgatgc
     2701 ggagcagcgc ctcgcgcgcg gtggctgccg ttgcgcggac atcgggaacg acctcgggcg
     2761 tcgtgaggct cccgtaggtt ccctcgtcgt agggcgtgaa gtcggtgtcc ccgagcagag
     2821 cccgcaccga cgaggcggga gcgcacagct cctcgctggc gagccggatc agctctgcgg
     2881 tgctcccctg ccccatctcc accttgcccg atgcgacggt gttccgacca tccgccccga
     2941 cgtgtagcca ggcgcggccc tcgtcgtcgg gcaccagcag gagcccgtcg ccgagccgat
     3001 cgaacagcgc ctcgtcgtcg cgcgcgaggt cccacgggga cgcgcgcgca tgctccagct
     3061 gcccgtccgg cgtttcgaca tccggcggcg aggtcgccgc cgcctgcggt cgagcttcgc
     3121 cctgggcggc gtgccgcaac cccgcgagaa tgcgggcgta agccccgcac cggcagat
//
LOCUS       DATCQR010000106         4386 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_161047, whole genome shotgun sequence.
ACCESSION   DATCQR010000106 DATCQR010000000
VERSION     DATCQR010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4386
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161047"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(27..605)
                     /locus_tag="VIN32_03540"
     CDS             complement(27..605)
                     /locus_tag="VIN32_03540"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8921687.1"
                     /translation="MLEIHPVTADRWEELAAFFGPSGAFAHCWCTWWRQTGAEFSQGE
                     TDGGAGNRTLMHEIVEAGSEPGLLADRDGHAVGWMSVAPRPQYGRIIRSRRIGPSLEE
                     AADETIWSVVCFWIPRRERGKGVANALLEASIDHARSRGAAVLEAYPVDTASGRQPSA
                     NLFTGTLTMFARAGFREVERPGGAQLVMRRTL"
     gene            complement(614..1291)
                     /locus_tag="VIN32_03545"
     CDS             complement(614..1291)
                     /locus_tag="VIN32_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921688.1"
                     /translation="MTEPMQPPRPRGGSRGAAVLGGALIVLGLIFFVGQALNMDIGEV
                     AWPIYVIVPGLALAALGLTQRHGSGLTIAGSIVTMIGLVLLYQNATDRFESWAYAWAL
                     VGPGGSGIGMLLYGTRSGDRKMARDGFWTILGAMGLFAVGLVFFEGVVGISGKRLPLP
                     SWVLPVAVIAVGVLVLLRGLTFRDRWDRAGEADDGAAPVPTPLGGGDESPEAVGAASA
                     EEPRAEG"
     gene            complement(1302..2282)
                     /locus_tag="VIN32_03550"
     CDS             complement(1302..2282)
                     /locus_tag="VIN32_03550"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HEY8921689.1"
                     /translation="MPRYPLLADILDARARLHWIALRTPLEDSPNLAAESGATEVRLK
                     LEMLQPTGSFKTRGAHNKVALVAAQEPDADLVTASSGNHGIAVATAAARHGMRLTVLV
                     GASVSPAKLERLRALESDSCVVEIFGRDSDDAEAEARRRDTERIAVYVPPYNDPDVIA
                     GQGTIGAEVLEEWPACDALVVPIGGGGLIAGIGLWARAVKPSLRLVGVQPSASPPMYA
                     YLESNSTKPMPIAPTLADGVSGNIERDSITWKMCRQLVDEVVLVDEEQIAEAMAWALE
                     VHHLVVEGSGALGIAALRARLGGLDGRRVGVVLTGRNVDGETLRRVLGQG"
     gene            complement(2311..2697)
                     /locus_tag="VIN32_03555"
     CDS             complement(2311..2697)
                     /locus_tag="VIN32_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921690.1"
                     /translation="MSTAVEPREWRRYGLGGPPEPWQHDAQRDIDRLATSYYLDVIEL
                     RGQILAAHPDEELRLRVDELHTTATRHKTEIDYTLRHWATPVERARVADRLGALMRIA
                     RRMDTFLHRPHGPLGVADPAPEPTVA"
     gene            2856..2930
                     /locus_tag="VIN32_03560"
     tRNA            2856..2930
                     /locus_tag="VIN32_03560"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2888..2890,aa:Gln,seq:ctg)
     gene            complement(2950..3714)
                     /locus_tag="VIN32_03565"
     CDS             complement(2950..3714)
                     /locus_tag="VIN32_03565"
                     /inference="COORDINATES: protein motif:HMM:NF013341.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIO1 family regulatory kinase/ATPase"
                     /protein_id="HEY8921691.1"
                     /translation="MQSATWPQRASPAWPDDEQYEDLPRGTLKSGKEAEIFLVERRYA
                     SGPRLLAHKRYRPRYPGKGQLRAEGFSNSTAYRGDSMYKAGWFLPTRDKRAVMGGSRY
                     GHELAGRLWPIQEWTMLRRAWDAGASVPYPVEQTDHGLLMEFIGDEVQAAPKLGQARL
                     SGSELKSAWEQLLASLSALTAAGLVHADLSAYNLLWWEGRLVVIDLPQAVEFTTNTDA
                     YDLLHRDIANVGEWFNRHGTAVDVEAVYAELLALAW"
     gene            complement(3942..>4386)
                     /locus_tag="VIN32_03570"
     CDS             complement(3942..>4386)
                     /locus_tag="VIN32_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020490348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8921692.1"
                     /translation="GLLVPYVRRLFKRRTDALLIGGVLGVVFLAILGLKPGFVVALAL
                     LALWACIGAVTRPMRSAFLNDVIPSEQRATVLSFDSLMGSAGGVVAQPILGRVADVSG
                     YATSYLVSAAVQIVALPLVFLARREKSPADPITDAPDAAEVIRPA"
BASE COUNT          689 a         1555 c         1490 g          652 t
ORIGIN      
        1 gagcatatgg tcgtgcatcg ctctggtcac agggtgcgcc gcatcaccag ctgggcgccg
       61 cccggccgtt cgacctcccg gaagccggcg cgcgcgaaca tcgtgagggt gccggtgaac
      121 aggttcgcgc tgggctgccg cccactcgcc gtgtcgacgg gataggcctc gagcacggcc
      181 gcaccgcgtg aacgcgcatg gtcgatcgac gcctccagca gcgcgttcgc aactcccttg
      241 ccccgctccc gacgcggaat ccagaagcag acgaccgacc agatggtctc gtccgctgcc
      301 tcctcgagag acggaccgat gcgtcgcgag cgaatgatcc ggccgtactg cgggcgagga
      361 gcgacgctca tccacccgac cgcgtgaccg tcgcggtcgg cgagcaggcc aggctcggaa
      421 cccgcctcga cgatctcgtg catcagcgtg cggttgccgg cgccgccgtc ggtctcgccc
      481 tggctgaact cggcccccgt ctgccgccac caggtgcacc agcaatgtgc gaaggcgccc
      541 gatggcccga agaaggcggc gagctcttcc cagcggtccg cggtgaccgg gtggatctcc
      601 agcatggacc ggctcagccc tctgcccggg gttcctcggc agacgcagcg ccgactgcct
      661 ccggcgattc gtcgcctcca cccagcggag tcgggaccgg cgccgcgccg tcatccgctt
      721 cccccgcacg gtcccagcga tcgcggaagg tcaggccacg cagcagcacc agcacaccga
      781 ccgcgatcac ggccaccggc agcacccagc tcggaagcgg cagccgcttc ccgctgatgc
      841 cgacgacccc ctcgaagaag acgagtccca cggcaaagag gcccatcgcg ccgaggatcg
      901 tccagaagcc gtcgcgcgcc atcttgcggt cgccgctgcg ggtcccatac agcagcatgc
      961 cgatgccgct gccacccggc ccgacaagcg cccaggcgta cgcccagctc tcgaagcgat
     1021 cggtcgcgtt ctggtagagg aggaccaggc cgatcatggt cacgatgctg cccgcgatcg
     1081 tcagtcccga gccgtgacgt tgggtcaggc caagtgccgc cagcgcaagg cccggcacga
     1141 tgacgtagat cggccaggcc acctcgccga tgtccatgtt cagtgcctgg ccaacgaaga
     1201 agatcaaccc gagcacgatc agggccccgc cgagcacggc tgccccccgg ctcccgccgc
     1261 gcggcctggg cggctgcatg ggctcggtca cgggaccgat gctacccctg gcccagcacg
     1321 cggcgcagcg tctcgccgtc cacgttgcgt cctgtgagga ccacgcccac gcggcggccg
     1381 tccagcccgc ccaaccgagc ccgcagcgcc gcgatcccca gggctccgct cccctcgacc
     1441 acgaggtggt gcacctcaag cgcccacgcc atggcctcgg caatctgctc ctcgtcgacc
     1501 agcaccacct cgtcgaccag ctggcggcac atcttccagg tgatgctgtc gcgctcgatg
     1561 tttccggaaa ccccgtcggc cagagtcggg gcgatcggca tcggcttggt ggagttcgac
     1621 tccaggtacg cgtacatcgg cggcgaggcc gagggctgca cccccaccag gcgcaggctc
     1681 ggcttgaccg ccctggccca caggccgatg ccggcgatca gcccgccgcc cccgattggg
     1741 accaccagtg cgtcgcaggc cggccattcc tcgagcacct cagccccgat ggtgccctgt
     1801 cccgcgatta cgtccgggtc gttgtacggg gggacgtaga cggcgatccg ctcggtgtcg
     1861 cgccggcgcg cctccgcctc ggcgtcgtcg gagtcgcgcc cgaagatctc gaccacgcag
     1921 ctgtcggact cgagcgctcg gagccgttcc agcttggcgg gggagaccga cgcgccgacg
     1981 agcaccgtca accgcatccc gtggcgcgcg gccgccgtcg cgacggcgat gccgtggttg
     2041 ccgctgctgg cggttaccag gtcggcatcg ggctcctggg ccgccacgag ggcgaccttg
     2101 ttgtgcgccc cgcgggtctt gaacgatccg gtcggctgga gcatctccag cttcagtcgc
     2161 acctcggtcg cgccgctctc ggcggcgagg ttcggcgagt cctcgagtgg cgtgcgcagg
     2221 gcgatccagt gcagccgcgc gcgagcgtcg aggatgtcgg ccaggagtgg gtagcggggc
     2281 attccccgga gtgtagggcc ggagttgccc tcaggcgacg gtgggttccg gtgctgggtc
     2341 ggcgacgccc agcggcccgt gcggccgatg caggaaggtg tccatgcgcc gggcgatccg
     2401 catcagcgcg ccgagccgat cggcgacacg ggcgcgctcc accggggtcg cccagtggcg
     2461 gagcgtgtag tcgatctccg tcttgtgtcg agtggcggtc gtgtggagct cgtcaacgcg
     2521 cagccgcagc tcctcgtcgg ggtgcgctgc caggatctgg ccgcgcagct cgatgacgtc
     2581 gaggtagtac gaggttgcca gacgatcgat gtcacgctgg gcatcgtgct gccatggctc
     2641 tggcggccca ccgaggccgt accgtcgcca ttcgcggggt tctactgcag tgctcatcgg
     2701 tgtcgaccgg cctctaactg gggcggtgcc tggatgccaa gaacctactg gcatggccga
     2761 tggcgtgcca ccgccaatcc gggctagtga tccccggacc tatgtacgcg agatcgggta
     2821 ttcaggggtg gtctggtaac atcggccggc actgttgggg tgtggccaag cggtaaggcg
     2881 cctgactctg gatcaggaga tcgaaggttc gaatccttcc gccccagcca cctgcgccgc
     2941 acggccgggt caccacgcca gcgccagcag ctcggcgtac accgcctcca cgtccaccgc
     3001 ggtgccgtgc cggttgaacc actcgccgac gttggcgatg tcccgatgca gcaggtcgta
     3061 ggcatcggta ttggtggtga actcgaccgc ctgcggcagg tcgatcacga ccagccggcc
     3121 ctcccaccag agaaggttgt aggccgagag atcggcgtgc accagccccg ccgcagtcaa
     3181 cgcgctcagg ctggccagca gctgctccca ggccgatttc agctcggagc ccgatagcct
     3241 ggcctggccc agcttggggg ccgcctgcac ctcgtcgccg atgaactcca tcagcagccc
     3301 atggtcggtc tgctccacgg gataaggaac cgatgcgcct gcgtcccacg cccggcgcag
     3361 catggtccac tcctggatcg gccagaggcg cccagccagc tcgtggccat agcggctgcc
     3421 gcccatcaca gcgcgcttgt cacgggtggg caggaaccag ccggccttgt acatcgagtc
     3481 gccgcgatac gcggtggagt tggagaagcc ctcggcacgc agctggccct tgccggggta
     3541 tcggggtcgg tatcgcttgt gggcgagcag gcgcggtccg ctggcgtagc gtcgctccac
     3601 caggaagatc tcggcctcct tgccggactt gagggtcccg cgcgggagat cctcgtattg
     3661 ctcgtcgtcg ggccaagcgg gggacgctcg ctgcggccag gtcgcggatt gcacgaaaaa
     3721 gcgctcctga gatcagggtc ggggatggat tggatcccga ccggaccagg agcgctacgg
     3781 acgcctcagt cagaggctgg cagcgttact cgaaacggcc gggagggact gatatgcggg
     3841 tccgcattgc gttcctcctc gtggctgcag ctcgtgatgg atcttgccgg gcagactatg
     3901 cacccggagc gggtcccccg caagagggct cggcgacatc gtcaggcggg cctgatcacc
     3961 tcggcagcat caggcgcgtc ggtgatcggg tccgcgggtg acttctcgcg ccgcgccagg
     4021 aagacaagcg gcagcgcgac gatctggacc gctgccgaga ccaggtagga ggtcgcgtag
     4081 ccgctgacat cggccacccg gcccaggatc ggctgggcga ccaccccgcc cgcggatccc
     4141 atcagcgagt cgaacgacag cacggtcgca cgctgctcgg agggaatgac gtcattgagg
     4201 aaggccgatc gcatcggcct cgtcaccgcg ccgatgcagg cccaaagcgc cagcagggcg
     4261 agcgccacga cgaaccccgg cttcagtccc aggatggcca ggaagacgac gcccagcacg
     4321 ccgccgatca ggagtgcatc ggtccgtcgc ttgaacagcc gacggacata gggaaccagc
     4381 agcccc
//
LOCUS       DATCQR010000107         4064 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_52701, whole genome shotgun sequence.
ACCESSION   DATCQR010000107 DATCQR010000000
VERSION     DATCQR010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4064
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52701"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..972)
                     /locus_tag="VIN32_03575"
     CDS             complement(<1..972)
                     /locus_tag="VIN32_03575"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY8921693.1"
                     /translation="MTDRFETLAVHAGAEPDEISGGVSPSIQMSATFRQDGVGKPRGG
                     YEYGRSGNPTRQALEAAIAELEGGTHGLAYASGLAATQNLLYLLEPGDRLILSDDAYG
                     GTWRLASKVWSRYGVQTHAVDLRDLDALGAVLSEAPAPRMVWVETPTNPLLKVVDISA
                     VASLAASAGALTVVDNTFATPFLQRPLELGADVVMHSATKYLSGHSDVISGLLATRRD
                     DLEERLRFHQNAAGAVPSPFDCWLVMRGMRTVAVRMERAAANAAAIAEWLAVRAEVSK
                     VYYPGLADHPQHELAKRQMRLPGAMLSFELVAGERAARRLAESTRLFT"
     gene            complement(1205..2749)
                     /locus_tag="VIN32_03580"
     CDS             complement(1205..2749)
                     /locus_tag="VIN32_03580"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019586024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEY8921694.1"
                     /translation="MTSIVQPPAPEAGKRSATERVNHWINGRRVAGSSGRSGPVYNPA
                     TGDLAREVDFASVEEVDAAVSVAKAAFPAWRATSLSRRTEILYRIRNLVEEHRAEIAA
                     HLTREHGKVPSDALGEVARGLENLEFATGIPHLLKGGFSEQVSTGIDVYQIRQPIGVA
                     AGITPFNFPAMVLMWMFGNAIACGNTFVLKPSEKDPSASVYLGELLQEAGVPDGVFNV
                     VHGDRVAVDRILEHPDIAAVSFVGSTPVARHIYETGTKNGKRVQALGGAKNHMVVLPD
                     ADIEMAADAAVSAAYGSAGERCMAISVVVAVGDVGDPLVDAIERRLPKIKVGPGSDED
                     AEMGPLITREHRDKVASYLESGPAQGATLRVDGRKHALYQEGQGFFLGVSLLDNVTPE
                     MECYRNEIFGPVLEIVRVPTYADAMQVIEDNPYANGTAIYTRDGGAARQFQFDVNVGM
                     VGINVPIPVPVAYYSFGGWKASLFGDLHMYGPEGVQFYTRAKVVTSRWPDPATSKVDL
                     GFPRTR"
     gene            complement(2778..3794)
                     /locus_tag="VIN32_03585"
     CDS             complement(2778..3794)
                     /locus_tag="VIN32_03585"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEY8921695.1"
                     /translation="MTAAVGGTPLVALDRLAAGLAPRVVAKLEHMNPGGSVKDRIALP
                     MIEAAERAGLLKSGGVIVEPTSGNTGVGLAMAAAVKGYRCIFVMADKQSEEKRALLRA
                     YGAEVVICPTDVPPDDERSYYRVSDRMAHEIPNAWKPDQYTNSANPQAHYATTGPEIW
                     DATDGRITHFVAGVGTGGTVSGAGRFLHEQNPKLVVVGADPAGSLFSGGDAHPYLTEG
                     VGEDFWPATYDTRVADKIIRVTDRDAFLTARQASAAEGILVGESGGMALWAALQVART
                     VEDADALFVILVPDSGRNYLGKLYNDDWLREVGIIGAHEQVADYDWRTTEPTLVVRRE
                     AQYP"
     gene            complement(3817..>4064)
                     /locus_tag="VIN32_03590"
     CDS             complement(3817..>4064)
                     /locus_tag="VIN32_03590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921696.1"
                     /translation="IYIMFYGVVRSYLETFRVENWLIAGIPTATWLGLGGFLLAGGFL
                     YLRHARGWGTPGAWIPQAEAQKREAQESEPSPEAQPG"
BASE COUNT          624 a         1409 c         1351 g          680 t
ORIGIN      
        1 ggtgaagagc cgggttgact ccgccaggcg acgagccgcg cgctcgccgg caaccagctc
       61 gaagctcagc atcgcgcccg gcaggcgcat ctgccgcttc gccagctcgt gctgcgggtg
      121 atcggccagg cccgggtagt acaccttcga gacttctgct cgcacggcga gccattccgc
      181 gatggccgcg gcgttggcgg cagctcgctc cattcgcacc gcgacggtgc gcatgccacg
      241 catcaccagc cagcagtcga agggagatgg gaccgcgccg gcggcgttct ggtggaaccg
      301 caggcgctcc tccaggtcgt cgcgacgcgt ggccagcagc ccgctgatca cgtccgagtg
      361 cccggagagg tacttggtgg ccgagtgcat gaccacatcc gcacccagct cgaggggccg
      421 ttgcaggaac ggcgtcgcga aggtgttgtc aacgacggtc agtgcgcccg cagaggctgc
      481 caggctcgcc acggcgctga tgtccaccac cttgaggagc gggttggttg gagtctccac
      541 ccacaccatg cgcggggccg gcgcctcgct cagcaccgcc cccagcgcgt cgaggtcgcg
      601 caggtcgacc gcatgggtct gcaccccgta tcggctccag accttcgagg ccaggcgcca
      661 ggtgccgcca tacgcatcgt ccgacaggat caggcggtct cccggctcga gcaggtagag
      721 caggttctgc gtcgccgcca ggccggatgc gtaggcaagg ccgtgcgtgc cgccctcgag
      781 ctccgcgatc gccgcctcca gcgcctggcg ggtggggttg ccgctccgcc cgtactcgta
      841 gccgccgcgg ggcttgccga cgccatcctg gcggaaggtg gcgctcatct ggatcgaggg
      901 cgacacgccg ccgctgatct catcaggctc cgcgccggcg tggaccgcca gcgtctcgaa
      961 tcggtcggtc atgcagcgcc attgtcgcag aggctggcct tggcaccctt gctgccaaat
     1021 tcacactagg caaccgcgag ggctgcctcg agagactgtt gccggacaaa ttcacatgag
     1081 gcgtgcacct ggcggcctgt tcgctgccag attcacgcaa ggtgggaatc cggctggttc
     1141 aagcagcgat cgttggcctg gcggtcaggt ggtgtgaatt tggcaggcca agcaaagcga
     1201 tgtgtcagcg agtgcgcggg aagcccaggt cgaccttcga cgtcgctgga tcgggccagc
     1261 gcgaggtgac caccttggcc cgggtgtaga actgcacgcc ctccggacca tacatgtgca
     1321 ggtcaccgaa gagcgatgcc ttccagccgc cgaagctgta gtacgcgacc ggcaccggga
     1381 tcggcacgtt gatgccgacc atcccgacgt tcacgtcgaa ctggaactgg cgcgccgcgc
     1441 cgccgtcgcg ggtgtagatg gcggtgccgt tggcatacgg gttgtcctcg atcacctgca
     1501 tcgcatcggc gtaggtcggg acgcgcacga tctcgagcac cgggccgaag atctcgttgc
     1561 ggtagcactc catctccggc gtcacgttgt cgagcagcga gacgcccagg aagaagccct
     1621 ggccctcctg gtagagggcg tgctttcgtc cgtcgacccg tagcgtcgcg ccctgtgccg
     1681 ggccgctctc caggtacgaa gcgaccttgt cgcggtgctc gcgggtgatg agcggaccca
     1741 tctcggcgtc ctcgtcgctg ccggggccga ccttgatctt gggcaggcgg cgctcgatcg
     1801 cgtcgaccag cgggtcgccc acgtcgccga ccgccacgac cacgctgatc gccatgcagc
     1861 gttcgccggc tgagccgtag gcggcggaga cggcggcatc ggcggccatc tcgatgtcgg
     1921 cgtccggcag gaccaccatg tggttcttgg cgccgcccag cgcctggacc cgcttcccgt
     1981 tcttcgtgcc ggtctcgtag atgtgccgcg cgacaggtgt cgagccaaca aatgagacgg
     2041 cggcgatatc ggggtgctcc aggatccggt cgaccgcgac acgatcgccg tgaacgacgt
     2101 tgaagacgcc atccgggacg cccgcttcct gaagcagctc accgagatag accgatgccg
     2161 atgggtcctt ctcggatggc ttgaggacga acgtgttgcc gcaagcgatg gcgttgccga
     2221 acatccacat cagcaccatg gccgggaagt tgaacggcgt gatacctgcc gccacgccga
     2281 tcggctggcg aatctggtag acgtcgatgc cggtgctgac ctgctcgctg aagccgccct
     2341 tgagcaggtg cgggatgccg gtcgcgaact ccaggttctc caggccccgg gccacctcgc
     2401 ccagggcgtc gctggggacc ttgccgtgct cgcgggtcag gtgcgcggcg atctcggcgc
     2461 gatgctcctc gaccaggttg cggatccgat acaggatctc ggtccggcgg ctgaggctgg
     2521 tcgcccgcca ggccgggaat gctgccttgg caaccgagac tgccgcatcg acctcctcga
     2581 ccgatgcgaa gtcaacctcc cgtgccaggt cccccgtcgc cgggttgtag accggcccgc
     2641 tgcgcccgct gctgccagcc actcgacgcc cgttgatcca gtgattgacg cgctcggttg
     2701 ctgaccgctt gccagcctcc ggggccggcg gctggacgat gctggtcatc gagctggaac
     2761 tccgtcgtgc gtgagtctca agggtactgc gcctccctgc gcaccaccag cgtcggctca
     2821 gtcgtccgcc agtcatagtc ggcgacctgc tcgtgggcac cgatgatccc gacctctcgc
     2881 agccaatcgt cgttgtacag cttgcccagg tagttgcgtc cgctgtcagg cacgaggatc
     2941 acgaacagcg catcagcgtc ttcgacggtt cgggcgacct ggagggccgc ccacagcgcc
     3001 atcccgcccg actccccgac caggatcccc tccgctgccg acgcctgccg agcggtgaga
     3061 aacgcgtcgc gatccgtaac ccggatgatc ttgtcggcga cccgtgtgtc ataggtggcc
     3121 ggccagaagt cctcgcccac cccctcggtc aggtaagggt gcgcgtcgcc gccggagaag
     3181 aggctccccg ccgggtcagc ccctaccacg acgagcttcg ggttctgctc atgaaggaac
     3241 cgtccggcac cgctgaccgt gcccccagtc cccacgccgg ccacgaagtg cgtgatgcga
     3301 ccatcggtcg cgtcccagat ctcggggccg gtggttgcgt aatgcgcctg cggattggcc
     3361 gagttcgtgt actggtcggg cttccaggcg ttggggatct cgtgcgccat tcggtcgctg
     3421 acccggtaat agctgcgctc gtcgtccgga gggacgtcgg tggggcagat cacgacttcg
     3481 gccccgtacg cccgcagcag cgcccgcttc tcctctgatt gcttgtcggc catcacgaag
     3541 atgcaccgat agcccttcac cgcggcggcc attgccagcc cgacgccggt gttgccgctg
     3601 gtcggctcca cgatgacgcc gccggacttc agcagcccgg cacgctcggc cgcctcgatc
     3661 atgggcaggg caatccggtc cttgaccgat ccgccggggt tcatgtgctc gagcttcgcc
     3721 accaccctcg gcgccagccc ggccgccagc cgatcgagcg caaccagcgg cgtaccgccg
     3781 acggctgcag tcacatcggg caggatgccc cgccggtcag ccaggctgcg cctccgggga
     3841 tggctcgctc tcctgcgcct cgcgcttctg agcttcggcc tgcgggatcc aggctcctgg
     3901 cgtgccccag ccgcgcgcat gacgcaggta gaggaatcct ccagccagga ggaaccctcc
     3961 cagccccagc caggtagcgg tcgggatgcc ggcgatcagc cagttttcca cgcggaaggt
     4021 ctcgaggtag gagcgaacta ctccgtagaa catgatgtag atgc
//
LOCUS       DATCQR010000108         9437 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_52789, whole genome shotgun sequence.
ACCESSION   DATCQR010000108 DATCQR010000000
VERSION     DATCQR010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 9437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9437
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52789"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="VIN32_03595"
     CDS             complement(<1..191)
                     /locus_tag="VIN32_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEY8921697.1"
                     /translation="MLNAPVLVLNQNYEPLNVCDIRRAFNLLGSEKAELLERNHQVIH
                     TPTLAIPAPSVIRLVYLVRR"
     gene            374..1255
                     /gene="pdxS"
                     /locus_tag="VIN32_03600"
     CDS             374..1255
                     /gene="pdxS"
                     /locus_tag="VIN32_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HEY8921698.1"
                     /translation="METSTWTTKVGLARMLSGGVIMDVVTADHARIAEEAGAVAVMAL
                     ERVPSDIRKFGGVARMSDPQLIEEIKGAVSIPVMAKCRIGHIAEAQILQQLEVDFIDE
                     SEVLTPADEEHHIDKHEFRVPFVCGCRDLGEALRRIGEGAAMIRTKGEAGTGNIVEAV
                     RHMRAVMKGIRELSGMREDELYAAAKELRAPLDVVRQTATLGELPVPNFSAGGVATPA
                     DASLMRQLGAQAVFVGSGIFKSENPDRVARAIVKATTHWDNPEILAEVSTGLGEPMHG
                     LELSEIPAEERLAVRGW"
     gene            1272..1898
                     /gene="pdxT"
                     /locus_tag="VIN32_03605"
     CDS             1272..1898
                     /gene="pdxT"
                     /locus_tag="VIN32_03605"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013423483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HEY8921699.1"
                     /translation="MAQTLPVPTPSQASAVRRPRIGILALQGDVREHATALRDVGAEP
                     VEVRLPRDLVGIDGLILPGGESTTMRRLIDLYGLREPIVALAHGGAPLYGTCAGMILL
                     ADRIADVDEPVLRLLDITVERNGYGRQLDSFEADLDIPSLGGEPLHGVFIRAPVVTEV
                     GPDVEVLARDPDGRPIAVRQGRVMATAFHPELTGDRRLHRLLLGLIEK"
     gene            1895..2887
                     /locus_tag="VIN32_03610"
     CDS             1895..2887
                     /locus_tag="VIN32_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921700.1"
                     /translation="MKDAAGSIRPARLTDRPALVALSRQVHEGSEGFRRSLGVPARSA
                     APPQISLAALMPSWLPLRSPSVHLVAEWDGQLVGSCRAVEEPHRDDWVITELDAADSP
                     MASEVRFALLQAVIEEGGKRDVARYHAACADVPENMELFGQVNFMAYASEEIWYRRPG
                     RLDGGRADDLQPATPADAWHLFDLWTHTTPPAIARIEAYGAQDWEPVGHEAVVPRSSL
                     NPILHFTDVQAWLLPEGQRVAGFAQHGACRAGPHYLRFLVRDGADGAAFLGAVLKAAG
                     KEALSAGILAPVRTYESTGRQAAEAAGFEAVGQVSVLVREVLASARQPAMVPAV"
     gene            2893..4665
                     /locus_tag="VIN32_03615"
     CDS             2893..4665
                     /locus_tag="VIN32_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HEY8921701.1"
                     /translation="MRKTASKVTKQETTDDLDALLEALPSEIVTRLRGFANRADLLEV
                     VLDLGRRPEARFTHGEEVLLEREIVEADIAHVIDHIGAFGGDNRAGIERTLHRISAIR
                     NRAGKVVGLTCRIGRAVFGTIDIIRDIVEGGQSVVLLGRPGIGKTTMLREVARVLADD
                     LGKRVIVVDTSNEIAGDGDIPHPGIGDARRMQVRVPAEQHAVMIEGVENHMPEVIVID
                     EIGTELEAAAARTIAERGVQLVGTAHGNTLDNLMLNPTLSDLVGGIQTVTLGDEEARR
                     RGTQKTVLERKAPPTFDVVVEIVERDSVIVHRNVAETVDAILRGHMVPPESRWREGGE
                     LRAATKYDYRIAESPTGIPTFAALEPTGGFGAYGRGRGNGGGMGLRPLPGRGGGGRDQ
                     GRGRALPGEPSESSDPRRHLEVAMRESSAPDTSSVGTKPVRSHRPLSLFGFGVSRKHL
                     EQAVRELGVPVHVARELDEADAVVTLRNYYRRKPSALRDAESGGIPIYVLKTNTIIQM
                     ENLLASLFDLEADPSEAALHETAEAIGLVQSSGNPMELAPQNAYVRRLQHQMAERNSL
                     MSRSRGTEPNRRVELLPEEGRAWR"
     gene            4670..5326
                     /gene="tmk"
                     /locus_tag="VIN32_03620"
     CDS             4670..5326
                     /gene="tmk"
                     /locus_tag="VIN32_03620"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018157177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEY8921702.1"
                     /translation="MNAPRRGRFITLEGPDGSGKTTAARHLAEWLRSRAEPTVLTGEP
                     GGTPLGEEVRRLVLHERNVSDDLDPRADALLFAAARAQHTARVILPALERGDNVVCAR
                     YLDSSLAYQGAAYGIEAGAIRQLQHFATYDTLPDMTILVDVPVEVGLARKRRGPWNRF
                     EDTEGDHFFEAVRAAYLALAEAEPKRYRVVDGSGSVDETDAAIRAAVEPLVFGAAEAR
                     "
     gene            5560..5889
                     /locus_tag="VIN32_03625"
     CDS             5560..5889
                     /locus_tag="VIN32_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HEY8921703.1"
                     /translation="MKLVTAVVHSEDAANLVDALLEKEFRATRLQSSGGFLKQGNATI
                     LVGVEDDQVDAVLEIVSANCHSRRQFVNPMPPIMEPGEFYMPYPVEVEVGGATVFVVP
                     VERFERL"
     gene            5901..6512
                     /locus_tag="VIN32_03630"
     CDS             5901..6512
                     /locus_tag="VIN32_03630"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEY8921704.1"
                     /translation="MAASAWLAALEPRAPDVVAVWRTIDDEQPVINGIAFRREIRRRA
                     GPGSAARILAQHEAIGDALEAVLTDLPDALLRAPGGEGDWNVAQAFAHTTGSRRWLAH
                     AAALAARGEWPADAPKVVPGVPGPADADIPTLLTLLGKSRRSLATSAEAIAGHEAEPC
                     PLDHPLVGRLRCGEWLLFAGVHDLMHLHQLHGLRAAEPDGRDD"
     gene            6505..7671
                     /locus_tag="VIN32_03635"
     CDS             6505..7671
                     /locus_tag="VIN32_03635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921705.1"
                     /translation="MTERPAPLVLVVDPGTIPDPDLPFGSYAAVGMAGRRRLAIELSR
                     RLIAAGSGVAALHPAKPAGGEAFHWGRWYAPAARALLVRVRAEGRPLDAIGYAGAGAL
                     ALADDALIEGIVSTIPGEAVANNRYSTDAFLVAGRPSGRLSLEDALGALEECSTDNAA
                     MHSLEAAGFAIRDLSTASWARFDVDTPLDLALLRLGARLPSTRRPDGVVAAFLEMAIL
                     PGGHSLEVPHLAEIGAVMRDRDAQLVVAGRVPSASWAYLETESACRVRCFIEERGMRS
                     ARDAVPHSLLADWMDRLGPADLVKELGTLGDAVILDSRVLMASRAGSSQATAWPPSEE
                     RFASDFLDAAPVTTPWLAELTEAAASSSVPFLLGGHALVSDGLRILVDAAWLGR"
     gene            7809..8168
                     /locus_tag="VIN32_03640"
     CDS             7809..8168
                     /locus_tag="VIN32_03640"
                     /inference="COORDINATES: protein motif:HMM:NF012717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEY8921706.1"
                     /translation="MDQTGLLYVIGFAIAASIFVFGTILFSWLITHRRRTPQKGLPYE
                     CGIDTIGDTWSRFGLAFYLYALLFVAFDVEIVFVYLWAVVVRVIAVQGIVLIGVFLGI
                     LLFGELYAWRKGDLIWR"
     gene            8300..8842
                     /locus_tag="VIN32_03645"
     CDS             8300..8842
                     /locus_tag="VIN32_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019552765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HEY8921707.1"
                     /translation="MEEHPQGGFHGLLEVIPTRADIVLDLIRANSLWPLLSGLSCCAI
                     EMMSTATSKNDIDRFGMFPFRASPRQADVLIVAGTLTTKMAGPLVRLWEQMPEPKWCV
                     AMGTCTTSGGRFKRSYSVVQGVDRVMPVDVYVPGCPPRPEGLIYGMMRLQELVKQRRG
                     EWSTYVDRGVQRIDWEGDVR"
     gene            8782..>9437
                     /locus_tag="VIN32_03650"
     CDS             8782..>9437
                     /locus_tag="VIN32_03650"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEY8921708.1"
                     /translation="MVDLRRSRRPADRLGRRRALTAPAKAGALVRLLLDRFPDLAAGL
                     WTDEDTVEVTIEGERNVALLRVLRDDPKLNFFFLADAAAVDYGTTERFEVVYHLYSDR
                     HPAWLRVRARVNRADPHIPTLTGLWEGAMFHEREMFDMMGIVFDGNRDLRRIYMSPDY
                     RSFPQRKDFLLPDDAADSSAAGVRPLERPETWDLTRLSDELAPGVVAHAEAEAEAEAE
                     A"
BASE COUNT         1418 a         3329 c         3306 g         1384 t
ORIGIN      
        1 cggcggacga ggtagacgag ccggatcacg gacggcgcgg gaatggcgag ggtcggcgtg
       61 tggatgacct ggtggttccg ctccagcagc tccgccttct ccgacccgag caggttgaag
      121 gcacgcctga tgtcgcagac gttgagcggc tcgtaattct ggttgaggac caggaccggt
      181 gcgttgagca agcggtgcct cccgtggcgc ggatgatagc aagcggctgc cgatcggctg
      241 acgaggccct ctggccaagg cctgagggtg ggaggccgac acgctcccga gtacaatgcg
      301 aggccagtgc ggcgcggcag cgccgccatc ggtctcaagc acgaagtcga cacgaagagg
      361 ctgcatggcc gccatcgaaa ccagcacctg gacaaccaag gtcgggctcg cccgcatgct
      421 ctccggcggc gtcatcatgg acgtcgtcac ggccgatcac gcgcggatcg ccgaggaggc
      481 cggcgcggtc gcggtcatgg cgcttgagcg cgtcccgtcc gacatccgca agttcggcgg
      541 cgtggcgcgc atgtccgacc cccagctgat cgaggagatc aagggcgcgg tcagcattcc
      601 cgtgatggcc aagtgccgca tcggccacat cgccgaggcc cagatcctgc agcagctcga
      661 ggtcgacttc atcgacgagt cagaggtgct gacgcccgcc gacgaggagc accacatcga
      721 caagcatgag ttccgggtgc cgttcgtgtg cggctgccgc gacctggggg aagccctgcg
      781 gaggatcggc gagggggcgg ccatgatccg taccaagggt gaggccggca ccggcaacat
      841 cgtcgaggcg gtgcgccaca tgcgcgccgt gatgaagggc atccgcgagc tgtccggcat
      901 gcgtgaggac gagctgtacg ccgcggccaa ggagctgcgc gccccgctcg acgtggtgcg
      961 ccagaccgcg accctgggcg agcttcccgt tcccaacttc tcggccggag gcgtcgccac
     1021 gccggcggac gcgagcctga tgcgccagct cggcgcgcag gcggtcttcg tagggagcgg
     1081 catcttcaag agcgagaacc ccgaccgcgt ggcgcgggcg atcgtgaagg ccaccaccca
     1141 ctgggacaac cccgagatcc tggccgaggt gagcacgggc ctgggcgagc cgatgcacgg
     1201 cctggagctc tccgagattc cggcggagga acgcctggcc gtccgcggct ggtaggggag
     1261 gcccagcacc gatggcgcag accctccctg ttcccacgcc ctcccaggcg tcagcggttc
     1321 gtcgcccgcg gatcggcatc ctggccctgc agggcgacgt ccgcgagcac gcaaccgcgc
     1381 tgcgggacgt tggcgcggag ccggtcgagg tgcgcctgcc tcgtgacctg gtgggcatcg
     1441 acggcctcat cctgcccggc ggcgagtcga ccaccatgcg caggctgatc gacctgtacg
     1501 gactgcgcga gccgatcgtg gcgctggcac atggcggcgc cccgctgtac ggcacctgcg
     1561 ccggcatgat cctgctcgcc gatcgcatcg ccgacgtcga cgagcccgtc ctgcgcctcc
     1621 tcgacatcac cgtcgagcgc aacggctacg ggcgccagct cgactccttc gaggccgatc
     1681 tcgacatccc ctccctcggc ggcgagccgc tgcacggtgt cttcattcgc gctcccgtcg
     1741 tgaccgaggt cggacccgac gtcgaggtgc tggcccgcga ccccgacggc cggccgatcg
     1801 ccgttcgcca gggccgcgtc atggcgaccg ccttccaccc ggagctcacc ggggatcgtc
     1861 ggctgcatcg gctcctgcta gggctcatcg agaagtgaag gacgcggccg gctcgatcag
     1921 gcccgcgcgg ctcaccgatc gtccggcgct ggtcgccctt tcgcgccagg tccacgaggg
     1981 gagcgaggga ttccgtcgct cgctcggcgt ccccgcgcgt tccgcggccc cgccccagat
     2041 cagcctcgca gccctgatgc cgtcctggct gcccctcagg tcgccatcgg tgcacctggt
     2101 ggccgagtgg gacggccagc tggtcggcag ctgccgagcc gtcgaggagc cgcaccgcga
     2161 cgactgggtg atcaccgagc tcgacgccgc cgactccccg atggccagcg aggtccgctt
     2221 cgccctgctg caggccgtga tcgaggaggg aggcaagcgc gacgtggcgc gctaccacgc
     2281 cgcgtgtgcc gacgtcccgg agaacatgga gctcttcggc caggtcaact tcatggccta
     2341 tgccagcgag gagatctggt accggcggcc gggccgcctc gatggcgggc gcgcggatga
     2401 cctgcagccc gccacccctg ccgacgcatg gcacctgttc gacctgtgga cgcacaccac
     2461 cccaccggcc atcgcgcgca tcgaggccta cggcgcccag gactgggagc cggtcgggca
     2521 cgaggcggtc gtcccgcgca gcagcctgaa tcccatcctg catttcaccg atgtccaggc
     2581 gtggctcctg ccggagggcc agcgcgtcgc cggcttcgcc cagcacggcg cgtgccgagc
     2641 cgggccgcat tacctgcgct tcctggtgcg cgacggcgct gacggcgcag ccttcctggg
     2701 cgccgtgctg aaggccgctg gcaaggaggc gctgtctgca gggatcctcg caccggtgcg
     2761 aacatacgag tcgacaggaa ggcaggccgc agaggcggcc gggttcgagg cggttggcca
     2821 ggtcagcgtg ctggtgcgcg aggtcctggc cagcgcccgc cagccggcga tggttccggc
     2881 ggtctagcca gcatgaggaa gacagcgagc aaggtgacca aacaagagac gaccgacgat
     2941 ctggacgcgc tgctcgaggc gcttccatcc gagatcgtga cccgcctgcg gggattcgcc
     3001 aaccgtgcgg acctgctcga ggtggtgctc gaccttggcc gccgtccgga ggcgcgcttc
     3061 acgcacggtg aagaggtgct cctcgagcgc gagatcgtcg aggccgatat cgcccacgtc
     3121 atcgatcaca tcggcgcctt cgggggcgac aaccgcgccg gcatcgagcg caccctgcac
     3181 cgcatcagcg ccatccggaa ccgcgccggc aaggtggtcg ggctgacgtg ccgcatcggg
     3241 cgcgccgtgt tcggcacgat cgacatcatc cgcgacatcg tcgagggcgg ccagagcgtg
     3301 gtgctactgg gacggccggg aatcggcaag accaccatgc tgcgcgaggt ggcccgcgtc
     3361 ctggccgacg acctcggcaa gcgcgtgatc gtggtcgaca ccagcaacga gattgccggc
     3421 gacggcgaca tcccgcaccc cggcatcggc gacgcgcggc ggatgcaggt gcgcgtcccg
     3481 gcagagcagc acgcggtgat gatcgagggc gtcgagaacc acatgcccga ggtgatcgtt
     3541 atcgacgaga tcgggaccga gctggaggca gccgcggcgc gcaccatcgc ggagcgcggg
     3601 gttcagctgg tcggcaccgc gcacggcaac acgctcgaca acctgatgct gaatccgacc
     3661 ctctcggacc tcgtgggcgg catccagacg gtcaccctgg gcgacgagga ggcacggcgg
     3721 cgcggcaccc agaagaccgt gctggagcgc aaggcacccc ccaccttcga cgtggtggtc
     3781 gagatcgtcg agcgcgacag cgtgatcgtg catcgcaacg tggcggagac ggtggacgcc
     3841 atcctgcgcg gtcacatggt gccgcccgag tcccgctggc gggagggcgg cgaactgcgg
     3901 gccgcgacta agtacgacta tcggatcgcg gagtcgccca ccggcatccc gaccttcgcg
     3961 gcgctcgagc cgacgggcgg cttcggggcg tatggccggg gtcgcggcaa cggcggcggc
     4021 atgggactcc ggcccctgcc cgggcgcggc ggaggcggtc gcgaccaggg acgcggccgg
     4081 gcgctgcctg gcgagccgtc agagtcgtcg gacccccgtc gccacctcga ggtggcgatg
     4141 cgggaatcaa gcgcgcccga cacgagcagc gtggggacga agccggtccg ctcccatcgg
     4201 cctctgagcc tgttcggctt cggggtcagc cgcaagcacc tcgagcaggc ggtgcgggaa
     4261 ctgggcgtgc cggtgcatgt cgcgcgcgag ctcgacgagg cggatgcggt cgtaaccctc
     4321 cgcaactact accgtcgcaa gccgtcggcg ctgcgcgacg ccgagtcagg cgggatcccg
     4381 atctacgtcc tcaagaccaa caccatcatc cagatggaga acctgctggc gagcctgttc
     4441 gacctggagg ccgatccgtc cgaggctgcg ctccatgaga cggcggaggc gatcgggctg
     4501 gtgcagtcga gcggcaaccc gatggagctg gccccgcaga acgcctacgt gcgccgcctc
     4561 cagcaccaga tggccgagcg caacagcctg atgagccgct cacgcgggac ggagccgaac
     4621 cgccgcgtcg agctcctccc cgaggagggg cgcgcgtggc ggtgagccgg tgaacgcgcc
     4681 ccgtcggggt cgcttcatca cgctggaggg gcccgacggg agcggcaaga caaccgccgc
     4741 gcggcacctg gccgagtggc tccgctcgcg cgcagagccg acggtcctga ccggcgagcc
     4801 gggcggcacg ccactcggcg aggaggtccg ccgcctggtc cttcacgagc gcaacgtctc
     4861 ggacgacctg gacccacgcg ccgatgccct cctcttcgcc gcggcccggg cccagcacac
     4921 cgctcgcgtc atcctcccgg cacttgagcg aggcgacaac gtggtctgcg cccgctacct
     4981 ggactcgtcg ctcgcctacc agggcgccgc ctacgggatc gaggccggtg ccattcgcca
     5041 gctccagcac ttcgccacct acgacacgct gccggacatg acgatcctgg tcgacgtgcc
     5101 ggtggaggtg ggccttgccc gcaagcgacg cggcccatgg aaccgcttcg aggacaccga
     5161 gggcgaccac ttcttcgagg cagtacgtgc cgcctacctc gcgctcgccg aggccgagcc
     5221 gaagcgctat cgcgtggtcg acggctcggg cagcgtcgat gagaccgatg ctgccatccg
     5281 cgcggcggtc gagccactcg tcttcggggc ggctgaggcg cgataagcgg gccatcggtg
     5341 cgattccact caccctgggc atcgcgaccc cgcccgcgag ccgcgataac tcggagtgat
     5401 attcccgcac tggaacactg ccgcctgtgc cgcctgatcg cccagcgtta tcaggcagcg
     5461 cagattgggc ggctgcggtg cgggagcata accctgggta atcgcaattc gccgtccgga
     5521 cgggtggccg ggggcgcttc cctgcggcat aatcggcctg tgaagctcgt gaccgcggtc
     5581 gtccacagcg aggatgcggc aaacctcgtc gacgccctgc tggagaagga attccgcgcc
     5641 actcggctgc agtcctccgg cggcttcctc aagcagggga acgcgaccat cctggtgggg
     5701 gtcgaggacg accaggtcga cgccgtgctc gagatcgtga gcgccaactg ccactctcgg
     5761 cgccagttcg tgaacccgat gccgccgatc atggagcccg gcgagttcta catgccgtac
     5821 ccggtcgagg tcgaggtcgg cggagcgacg gtcttcgtgg tccccgtcga gcgcttcgag
     5881 cgcctctagg accgataccg atggcggcct ccgcatggct ggccgccctg gagccgcgcg
     5941 ccccggacgt ggtcgcggtc tggcgaacga tcgacgacga gcagcccgtc atcaacggaa
     6001 tcgccttccg tcgcgagatc cgccggaggg ctggtccggg cagcgcggcc aggatcctgg
     6061 cccagcacga agccatcggg gatgcgcttg aggcggtctt gaccgacctg cccgatgcgc
     6121 tcctgcgggc gccgggtggc gagggcgatt ggaatgtcgc ccaggccttc gctcacacga
     6181 ccggctcgcg gcggtggctg gcccacgccg cggcactcgc cgcgcgtggc gagtggcctg
     6241 ccgacgcgcc caaagtcgtg ccgggggtgc caggccccgc cgacgccgac attccgaccc
     6301 tcctgaccct gcttggcaag agccgccgct cgctggcgac ctcggccgag gcgatcgccg
     6361 gacacgaggc tgagccatgc cccctcgacc atccactggt gggccgcctg cgctgcggag
     6421 agtggctcct cttcgccggc gtccacgacc tgatgcacct ccaccagctg catggcctgc
     6481 gagctgccga gccggacgga cgggatgact gagcgaccgg cgcccctcgt gctggtggtc
     6541 gaccccggca ccatccccga tccggatctt cccttcgggt cgtatgccgc cgtgggcatg
     6601 gccgggcggc ggcggctggc gatcgagctg tcgcgccgcc tcatcgcagc cggctccgga
     6661 gtggccgcgc tgcacccggc caagccggcc ggcggagagg cattccactg gggacgctgg
     6721 tacgcgccgg cagcccgcgc gctcctggtc cgggtccggg cggaggggcg gccactcgac
     6781 gcgatcggct acgccggggc cggagccctt gccctcgccg atgacgcgct gatcgagggg
     6841 atcgtctcga ccattcccgg cgaggcggtc gccaacaacc gctactcgac cgatgccttc
     6901 ctcgtcgccg ggcggccatc gggccgcctc tccctggaag atgccctggg ggcactcgag
     6961 gaatgctcca cggacaacgc cgcgatgcac tccctggagg cggcggggtt cgcgatccgc
     7021 gacctgtcga ccgcgagctg ggcgcggttc gacgtggaca cgcccttgga tcttgccctc
     7081 ctgcgccttg gggcccgact gcccagtacg cgacgccccg acggggtggt ggccgccttc
     7141 ctggagatgg cgatcctgcc cggtggccac tcgctcgagg tcccgcacct ggccgagatt
     7201 ggcgccgtca tgcgcgaccg ggatgcgcag ctggtcgtgg ccggtcgcgt cccgtcggcc
     7261 tcctgggcct acctggagac ggagagcgcc tgccgcgtgc gctgcttcat cgaggagcgc
     7321 ggcatgcgtt cggcgcgcga cgccgtcccc cactcgctcc tggccgactg gatggaccgt
     7381 ctcggcccgg cggacctggt caaggagctc ggcaccctgg gtgacgcggt gatcctggac
     7441 agccgcgtgc tgatggccag tcgcgccgga tcgtctcagg ccacagcctg gccgccttcc
     7501 gaggagcgct tcgcctccga cttcctggac gcggcgccgg tcacgacacc ctggctggcc
     7561 gagctgacgg aggccgccgc aagctcatcg gtccccttcc tgctgggcgg tcatgccctc
     7621 gtcagcgacg gcctgcgcat cctcgtggac gccgcctggc tgggccggta gccaccctca
     7681 acgaccgcct atccggctgc tagactgttc gtgaagctgc ccttcgcgat gagcgtgggc
     7741 ggccgacgaa acgagcacca accgtgcggg accccaggtc ctgcgcccga gcaactcacg
     7801 gagctggcat ggatcagacc ggactgctct acgtcatcgg cttcgcgatc gccgcgtcga
     7861 tcttcgtctt cgggacgatc ctcttctcat ggctgatcac gcaccgccgt cggactcccc
     7921 agaaggggct tccctacgaa tgcgggatcg acaccatcgg cgatacctgg tcgcgcttcg
     7981 ggctcgcctt ctacctgtac gcgctgctgt tcgtggcctt cgacgtcgag atcgtcttcg
     8041 tctacctgtg ggcggtcgtg gtgcgggtca tagcagtgca ggggatcgtg ctgatcggcg
     8101 tcttcctggg gatcctgctc ttcggagagc tgtatgcctg gcgaaagggt gacctgatat
     8161 ggcgttgaag acgactcccg accctcagac cgtgcccgag ccaagcatgg ctgaggctga
     8221 ccgcgtcctc accggcggca agcctccgat cgtgatcccg gaccaggccg gcgacgaccc
     8281 gatcctgacc gagttcggga tggaggagca cccgcagggc ggcttccacg gcctcctcga
     8341 ggtgatcccc acccgcgcgg acatcgtcct cgacctgatc cgggccaact cgctgtggcc
     8401 gctcctgtcg gggctcagct gctgcgccat cgagatgatg agcacggcca ccagcaagaa
     8461 cgacatcgac cggttcggca tgttcccgtt ccgcgcctcg ccgcgccagg cggacgtgct
     8521 gatcgtggcg gggacgctga cgaccaagat ggcaggcccg ctggtgcgcc tctgggagca
     8581 gatgcccgag ccgaagtggt gcgtggcgat gggcacctgc accacctcgg gcggtcgctt
     8641 caagcgcagc tacagcgtgg tgcagggggt cgaccgggtg atgccggtcg acgtctacgt
     8701 gccgggctgc ccgccccgcc ccgagggcct gatctacggg atgatgaggc tccaggagct
     8761 ggtcaagcag cggcgcggcg agtggtcgac ctacgtcgat cgcggcgtcc agcggatcga
     8821 ctgggaagga gacgtgcgct gaccgcaccg gccaaggcgg gagccctggt ccggctcctg
     8881 ctcgatcgct tccccgacct ggccgcggga ctgtggaccg acgaggacac cgtcgaggtc
     8941 acgatcgagg gcgagcgcaa cgtggcactg ctccgggtcc tgcgcgacga tcccaagctc
     9001 aacttcttct tcctggccga tgccgcggcg gtcgactacg gcacgaccga gcgcttcgag
     9061 gtcgtctacc acctgtactc ggaccgtcat cccgcctggc tccgcgtccg cgcccgggtg
     9121 aaccgcgccg accctcacat cccgaccctc accggcctgt gggaaggggc gatgttccac
     9181 gagcgcgaga tgttcgacat gatggggatc gtgttcgacg gcaaccggga cctgcgccgg
     9241 atctacatgt cgccggacta ccggagcttc ccgcagcgca aggacttcct cctgcccgat
     9301 gacgccgccg actcgtcggc ggcgggtgtg cgtccgctcg agcgtcccga gacctgggac
     9361 ctgacccgtc tctccgacga gctggcgccc ggcgtcgtcg cccatgccga ggcagaggca
     9421 gaggcagagg cagaggc
//
LOCUS       DATCQR010000109         2148 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_83783, whole genome shotgun sequence.
ACCESSION   DATCQR010000109 DATCQR010000000
VERSION     DATCQR010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2148
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83783"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1485)
                     /gene="nuoL"
                     /locus_tag="VIN32_03655"
     CDS             complement(<1..1485)
                     /gene="nuoL"
                     /locus_tag="VIN32_03655"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005141.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEY8921709.1"
                     /translation="MTVESLIALIPALPLAGFLFAVLLGARIDRFPAHGHDAHGPGHA
                     DDHGSHSGHGGESHDTHSEVDESAFRPIPSEEEQRATSPHAGHADDLANSNGADGVIP
                     DDLNDGLGQTGHVVEGGPRPVYRSWWVPTALVAVMWIFSMVVFANVIFGGHIYHVSLY
                     EWIGSGSFHVQIAFLVDGLTAMLLLVVSTVGLLVHVYSIGYMDGDRGLWRFFAYLNLF
                     MFSMLLLILGDNFLMLYVGWEAVGLCSYALIGFWYKKPSAAGAAKKAFIVNRVGDFGF
                     GLGIMMIWTLLGTLDFEQVFATIGTLDPTAVTVIALLLFAGAVGKSAQFPLHVWLPDA
                     MEGPTPVSALIHAATMVNAGVYMVARANPIFAESPVAMWVVASIGVFTAIFAAAIALT
                     QNDIKKVLAYSTVSQLAYMFFALGIGAWVAAIFHLVSHGFFKGLLFMGSGSVIHGAAG
                     EQDMRLMGGLRAKMLWTYLTMVVGSLALAGIPIFAGFFSKDEILG"
     gene            complement(1490..1798)
                     /gene="nuoK"
                     /locus_tag="VIN32_03660"
     CDS             complement(1490..1798)
                     /gene="nuoK"
                     /locus_tag="VIN32_03660"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006680140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEY8921710.1"
                     /translation="MGLQAYLVVSAMLFGIGMFGFLARRNAILMLISIELMLNAVNLS
                     LVAFNAYDYYSPNDTGAIFALLVMAVAAAEATVGLALVIAIIRTRQTPEVDEYSDLKE
                     "
     gene            complement(1798..>2148)
                     /locus_tag="VIN32_03665"
     CDS             complement(1798..>2148)
                     /locus_tag="VIN32_03665"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEY8921711.1"
                     /translation="AAAQVLIYLGAISVLILFAIMLTQAGDANLPSPFHKQVWAAALV
                     SLVVVGLIGWAVTATDWGNATVVLAINIDAMATALFTDYALPFEILSMLLLAAIIGAI
                     YLARRPEDDERANS"
BASE COUNT          409 a          688 c          733 g          318 t
ORIGIN      
        1 gcccaggatc tcgtccttgc tgaagaagcc cgcgaagatg gggatcccgg ccagcgccag
       61 cgagccgacc accatggtga ggtaggtcca cagcatcttc gcccgcaggc cgcccataag
      121 gcgcatgtcc tgctcaccgg ccgcgccgtg gatcaccgag ccagagccca tgaacaggag
      181 ccccttgaag aagccgtggc tgacgaggtg gaagatggcc gccacccagg caccgatccc
      241 cagcgcgaag aacatgtagg ccagctggct gacggtcgag taggcgagca ccttcttgat
      301 gtcgttctgg gtcagcgcga tggccgccgc gaagatggcg gtgaagacac cgatgctggc
      361 cacaacccac atggcgaccg ggctctccgc gaagatgggg ttggcgcggg cgaccatgta
      421 gaccccggcg ttgaccatgg tggcggcgtg gatcagggcg ctgaccgggg tcgggccttc
      481 catcgcgtcc ggtagccaga cgtggagcgg gaactgggcg ctcttgccga ccgcgccggc
      541 gaacaggagc agggcgatca cggtcaccgc ggtgggatcg agcgtgccaa tcgttgcgaa
      601 gacctgctca aagtcgagcg tgccgagcag ggtccagatc atcatgatcc cgagcccgaa
      661 gccgaagtca ccgacccggt tgacgatgaa cgccttcttg gcggcacccg ccgccgaggg
      721 cttcttgtac cagaagccga tgagcgcgta gctgcacagg ccgaccgcct cccagccgac
      781 gtatagcatc aggaagttgt cgcccaggat cagcagcagc atgctgaaca tgaacaggtt
      841 caggtacgcg aagaagcgcc acagcccgcg gtcgccgtcc atgtagccga tcgaatagac
      901 atggaccagc agaccaaccg tgctgacaac cagcagcagc atcgcggtca ggccgtccac
      961 caggaatgcg atctggacgt ggaagctgcc ggagccgatc cactcgtaca gcgagacgtg
     1021 gtagatgtgc ccgccgaaga tgacgttggc gaagacgacc atgctgaaga tccacatgac
     1081 cgcgaccagg gcggtcggca cccaccacga gcggtagacc ggccgcggtc cgccctcgac
     1141 cacgtggccc gtctggccca ggccgtcatt caggtcgtcg gggatgaccc catcggcgcc
     1201 gttgctgttg gccaggtcgt cggcgtgtcc ggcatgcggc gaggtggccc gctgctcttc
     1261 ctcggagggg atcggccgga aggccgactc gtctacctcg gaatgcgtgt cgtgcgactc
     1321 gccaccatgg ccggagtggc tgccgtggtc gtcggcgtgt ccggggccat gcgcgtcgtg
     1381 gccgtgcgcc gggaagcggt cgatgcgcgc ccccaacagg accgcgaaca ggaagccggc
     1441 cagcggtagc gccgggatga gcgcgatcag cgactcgacg gtcatcggct cattccttga
     1501 ggtccgagta ctcgtccacc tcgggcgtct ggcgggtgcg gatgatggcg atcaccagcg
     1561 ccagccccac cgtcgcctcg gccgccgcga cggccatcac cagcagggcg aagatcgcgc
     1621 cagtgtcgtt gggcgagtag tagtcgtaag cgttgaaggc gaccagcgag aggttcaccg
     1681 cgttgagcat cagctcgatg ctgatcagca tcaggatcgc gttgcggcgc gccaggaacc
     1741 cgaacatccc gatcccgaac agcatcgccg agacgaccag gtaggcctgg aggcccatca
     1801 gctgttggcc ctctcgtcgt cttcagggcg tcgagcgagg tagatggcgc cgatgatggc
     1861 ggccagcagc agcatgctca ggatctcgaa cggcagcgcg taatcggtga acagcgccgt
     1921 ggccatggcg tcgatgttga tggcaagtac gacggtcgcg ttgccccaat cggtcgcggt
     1981 gacggcccag ccgatgagcc cgaccaccac cagcgacacg agcgcagccg cccaaacctg
     2041 cttgtggaac ggcgatggca ggttggcgtc cccggcctgg gtcagcatga tggcgaacag
     2101 gatcaggacg ctgatggcgc cgaggtagat gagcacctgc gccgccgc
//
LOCUS       DATCQR010000110         6925 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_37246, whole genome shotgun sequence.
ACCESSION   DATCQR010000110 DATCQR010000000
VERSION     DATCQR010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6925
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37246"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2037)
                     /gene="leuS"
                     /locus_tag="VIN32_03670"
     CDS             complement(<1..2037)
                     /gene="leuS"
                     /locus_tag="VIN32_03670"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEY8921712.1"
                     /translation="MTERGERFDPRTYESGWRERWAADDLYRADDDDPREKHYLLTMY
                     PYPSGDLHIGHWYAATGPDIVARMRRMQGLNVMFPMGFDSFGLPAENAAIDRGVHPWE
                     WTEASMDRMRTQFRSMGCMFDWSREVVTSDPSYYRWTQWLFLQFFKAGLAYRAMAPVD
                     WCPKDQVVLAREQVEGADRHCWRCGTPVIKRDLEQWFFRTTRYADELLDFSKIDWPEP
                     IRLMQTNWIGRSEGAEIDFAVEADGVAPIRVFTTRPDTIFGATFMVLAPEHPAVAALT
                     PADRRAEVEAYVAAARRETEIERMGTERERSGVFIGAHAVNPMTEERIPIWIADYVLP
                     TYGTGAVMGVPAHDERDYAFATSYQLPIRYVVVPRDGGQPEGEAFLPHTDDEVLVDSG
                     EFTGMAASDAIAAITEELSERGQGAAAITYRIRDWLISRQRYWGAPIPIVYCDEHGPQ
                     PVPEDQLPVLLPRDVDFAPTGVSPLQSHAEFLNASCPVCGKPARRETDTMDTFVDSSW
                     YFLRYCSPHDETRAWDPEAVMLWMPVDLYTGGAEHAVLHFLYARFFTKALRDMQLLDF
                     DEPFSAVRNQGQILGADHHRMSKSRGNVVNPDDLTSRFGSDTVRCFLMFIGPWDQGGP
                     WNPSSIEGVHRFLGRVWAVAQATGGGHPRRIPGMAVDSQGGWPPSSGEVPSQEMA"
     gene            complement(2034..2855)
                     /locus_tag="VIN32_03675"
     CDS             complement(2034..2855)
                     /locus_tag="VIN32_03675"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY8921713.1"
                     /translation="MTTETTDNPAARGRSFDAWADEYDRYRPTYPEALFAHIARELRL
                     PRRPHVADLGAGTGRASIAMASIGWRIAAVEPGEPMLDVLRSRAVEDGLSVETVQATA
                     EATGLDSAAFDLATAAQAFHWFDKPAALTEIARIVRPGGGVALFWNVRDEERSPFVAD
                     YHRILERYGGVAEGRYLQAGRSSGRSETREVLEAAEGFDEPHLHELHHELPATAEGFI
                     GMAFTASYVRALEPEEQARFRAETLDLLARHGHRGDEPFLVPYRIDLWIARRSVT"
     gene            complement(2852..3622)
                     /locus_tag="VIN32_03680"
     CDS             complement(2852..3622)
                     /locus_tag="VIN32_03680"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY8921714.1"
                     /translation="MTLEPGSLDAYLPDYPDALFELLSRELSLPERPLVVDVGAGTGR
                     ASLAMARLGWHVTAVEPDGPALDVLSARASREGLEVATIQATAEQTGLHAASVDLVTA
                     AHAFHWFVKRAALAEMARITRRGGGVALFWNVRDRERSPFVAGYQDLLDRYGVAKRLY
                     LEAERASGRATREALAGAEGLGSPRLHRVWHDMSMRPNDFLKISFAPAFVRVFSSERQ
                     DAFRTEVVELLAHHGFDGEAPVTIPYRIDCWIATRSTP"
     gene            complement(3619..4179)
                     /locus_tag="VIN32_03685"
     CDS             complement(3619..4179)
                     /locus_tag="VIN32_03685"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8921715.1"
                     /translation="MTITEAQPRTAKDGRPWTLRPARPTDARGLASLFAEVRREGRWL
                     VTPASAVSQPSEAFYIGEMIRDSSSLALVAEADGDVVGNVLVSVERSVVSDHVGTLSI
                     CVKDGWREVGIGSALVEAAKAWAAEGGLAKVALGVFPDNDRAIAVYEHAGFEREGLRR
                     RQYRVPGGEYRDELLMAWFPPEGDRR"
     gene            complement(4291..4366)
                     /locus_tag="VIN32_03690"
     tRNA            complement(4291..4366)
                     /locus_tag="VIN32_03690"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4331..4333),aa:Ala,seq:ggc)
     gene            complement(4561..4998)
                     /locus_tag="VIN32_03695"
     CDS             complement(4561..4998)
                     /locus_tag="VIN32_03695"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY8921716.1"
                     /translation="MRRVQRTAQMAATPAEVYAFLAEPANLPRWQAGIVAAERTSPPP
                     TAVGSTARVVLELMGQQVTAEITVREAEPERRLALASSVSGMGIVATLDLVAHDGGTE
                     ITLASEVRAENIFMAPLERMVTDAAERDLDASLTRLEAALALQ"
     gene            5121..5897
                     /locus_tag="VIN32_03700"
     CDS             5121..5897
                     /locus_tag="VIN32_03700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921717.1"
                     /translation="MLRRFAPLVVAMLAVLVACQSTPGITDPVDIIGQGLDATANLKS
                     LHVSLALDGTFKVPNGGGSFGLNATKLEADIDLKAKSLRATLAVPAFLNLTADAIVIG
                     QDVWYRTSLGGTKWFHQANDVMGSPSPSASLDPVAMVAKVKEALATEGVVTKKLADVA
                     CGDRQCYQVSVTVPSALMKTAGAAASMDPSSVFGDAMVINLLFDREKLWLTEVSTSVD
                     STTVGTIKATLSFSNFDEAVDFSPPPSPDVTEGEFTIPGM"
     gene            complement(5939..6691)
                     /locus_tag="VIN32_03705"
     CDS             complement(5939..6691)
                     /locus_tag="VIN32_03705"
                     /inference="COORDINATES: protein motif:HMM:NF024934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEY8921718.1"
                     /translation="MRSNSHPDAPTVDPRANHLLRGLTDEEFARIHAELEPVRLERSA
                     ELESPNERIEFVYFPTSGVASIVALEEGGESVDTAMIGREGMTGLPVFLGTGQSPVRT
                     IVQVPLTGVRMRSEVLVDELRRGGTLVRMLQRHAQVVMVTMAQLILCNRMHRLDQRAA
                     RWLLQVDERVDEKPFHVTQEFLAQMIGVRRPGLSVVMRQFKDAGLVNYARGQISIANH
                     EGLAEKSCNCINVIATEARRLRDLERAIDAAS"
BASE COUNT         1101 a         2407 c         2294 g         1123 t
ORIGIN      
        1 ggccatctcc tggctcggca cctctccgct gctgggcggc catccgccct ggctgtcaac
       61 cgccatgccc gggatccgcc ggggatggcc cccgcccgtc gcctgggcca ccgcccagac
      121 gcggcccagg aagcgatgga ccccttcgat gctcgaggga ttccacggcc cgccctggtc
      181 ccagggtccg atgaacatca ggaagcagcg gacggtgtcc gagccgaagc gcgaggtcag
      241 gtcgtcgggg ttgaccacgt taccgcgtga cttgctcatg cgatggtggt ctgcgcccag
      301 gatctgtccc tggttgcgca ccgcgctgaa cggctcgtcg aagtcgagca gctgcatgtc
      361 gcgcagggcc ttggtgaaga agcgggcgta caggaagtgc aagacggcgt gctcggcgcc
      421 gccggtgtag aggtcgaccg gcatccagag catcaccgcc tccgggtccc acgcccgcgt
      481 ctcgtcgtgg ggcgagcaat agcgcaggaa ataccacgac gagtcgacga aggtgtccat
      541 ggtgtcggtc tcgcggcgcg ccggcttgcc acacaccggg caggaggcgt tcaggaactc
      601 ggcatgggac tgcagcgggc tgaccccggt gggggcgaag tcgacgtcgc gcggcaggag
      661 caccggcagc tggtcctccg gcaccggctg cgggccgtgc tcgtcgcagt acacgatcgg
      721 gatcggtgcg ccccagtagc gctgccggct gatcagccag tctcggatgc gataggtgat
      781 ggccgccgct ccctggccac gctcggacag ctcctccgtg atggcagcga tcgcgtcgga
      841 cgccgccatg cccgtgaact cgccggagtc gaccagcacc tcgtcgtcgg tatgcggcag
      901 gaaggcctcg ccctccggct gcccaccgtc ccgcgggacc acgacgtagc ggatcggcag
      961 ctggtagctg gtcgcgaagg cgtagtcgcg ctcgtcgtgg gcggggacgc ccatcaccgc
     1021 cccggtccca taggttggca gcacgtagtc ggcgatccag atcgggatcc gctcctcggt
     1081 catggggttg accgcatgtg caccgatgaa gacgccgctc cgctcgcgct ctgtgcccat
     1141 ccgctcgatc tccgtctcgc gtcgggccgc ggccacgtag gcctcgacct cggcacgtcg
     1201 gtcagcgggt gtgagcgccg cgacggcagg gtgctccgga gccaggacca tgaaggtcgc
     1261 cccgaagatg gtgtcgggac gggtcgtgaa gacgcggatc ggcgcgacgc catcggcctc
     1321 caccgcgaag tcaatctcgg cgccctcgct gcgaccgatc cagttggtct gcatcaggcg
     1381 gatcggctcc ggccaatcga tcttgctgaa gtccaacagc tcgtccgcgt agcgagtggt
     1441 gcggaagaac cactgctcca ggtcgcgctt gatgaccggc gtgccgcagc gccagcagtg
     1501 gcgatcggcg ccttccacct gctcgcgcgc caggaccacc tggtccttgg ggcaccagtc
     1561 gaccggcgcc atagcccgat acgccagccc ggccttgaag aactgcagga acagccactg
     1621 ggtccagcgg tagtaggacg ggtcgctggt gaccacctcg cgcgaccagt cgaacatgca
     1681 gcccatcgag cggaactggg tccgcatccg atccatgctc gcctcggtcc actcccaggg
     1741 atgcacgcca cggtcgatgg ccgcgttctc ggccggcagg ccgaacgagt cgaagcccat
     1801 cgggaacatg acgttcaggc cctgcatgcg gcgcatgcgc gccacgatgt ccggtccggt
     1861 ggccgcatac caatgcccga tgtgcaggtc ccccgacggg tacgggtaca tggtcagcag
     1921 gtagtgcttc tcccgcgggt cgtcgtcatc ggcccgatac aggtcgtcgg cagcccaccg
     1981 ctcccgccac ccggattcgt acgtcctcgg atcgaaccgc tcgccacgct cggtcatgtc
     2041 acgctccttc gtgcgatcca caggtcgatt cgatagggaa ccaagaacgg ctcatccccg
     2101 cgatgtccgt ggcgcgccag caggtcgagc gtctcggctc gaaagcgagc ctgctcctcc
     2161 ggctcgagcg cacggacgta cgaggccgtg aacgccatgc cgatgaatcc ctctgcggtt
     2221 gccggcagct cgtgatgtag ctcgtgaagg tgcggctcat cgaatccctc agccgcctcg
     2281 agcacctcgc gggtctcgga gcgtcccgac gagcgcccag cctggaggta cctaccctcg
     2341 gccacacctc catagcgctc caggatgcgg tggtagtcgg caacgaacgg cgagcgctcc
     2401 tcgtcacgca cgttccagaa cagggcgacg ccgccacctg gtcggacgat gcgggcgatc
     2461 tccgtcaggg cagcaggctt gtcgaaccaa tgaaaggcct gtgccgccgt ggccaggtcg
     2521 aacgccgcgc tgtccagccc ggtcgcctcg gcggttgcct gaaccgtctc gaccgacagg
     2581 ccgtcctcaa cggcccgcga acggaggaca tccagcatcg gctcgccggg ttcgacggca
     2641 gcgatgcgcc agccgatgct ggccatcgcg atgctcgccc ggccggttcc cgcgcccagg
     2701 tcggccacgt gcgggcgccg cgggagtctc agctctcggg cgatgtgcgc gaacagggct
     2761 tccgggtacg tcggtcggta acggtcgtac tcgtccgccc aggcgtcgaa gctccgccca
     2821 cgggcggccg ggttgtcggt cgtctccgtc gtcatggagt gctccttgtg gcgatccagc
     2881 agtcgatgcg ataggggatg gtgaccggcg cctcgccgtc gaagccgtga tgggcaagga
     2941 gctcgacgac ctcggtgcgg aaggcatcct ggcgctcgga agagaagacg cgcacgaacg
     3001 ccggcgcgaa gctgatcttg aggaagtcgt tgggtcgcat cgacatgtcg tgccacaccc
     3061 gatggaggcg cggtgagccc aagccttccg cgccggcgag cgcctctcgc gtggcacgcc
     3121 cgctcgcgcg ctccgcctcg aggtatagac gcttcgccac gccgtagcgg tcgaggaggt
     3181 cttggtagcc ggcgacgaac ggcgaccgct cgcggtcgcg tacgttccag aacagcgcca
     3241 cgccgccgcc ccggcgggtg atgcgagcca tctcggccag ggccgcccgc ttgacgaacc
     3301 agtggaacgc gtgtgcggcc gtcaccaggt cgacgctcgc cgcgtggagg cccgtctgct
     3361 ccgcggtggc ctggatggtg gccacctcca gcccctccct cgaagcgcga gcgctgagca
     3421 cgtcgagtgc tggtccatcg ggctccaccg ccgtgacgtg ccagcccagc cgtgccattg
     3481 ccagcgaggc gcgaccggtg ccggcgccga catccaccac cagcgggcgc tccggcaagc
     3541 tcagttcccg gctcaagagc tcgaacaacg catccgggta atcgggcaag tacgcgtcga
     3601 ggctcccggg ctcgagggtc accttcggtc accttctggt gggaaccagg ccatcagcag
     3661 ctcgtctcga tactcgccac cggggacgcg gtattggcgt cggcgcagac cctcgcgctc
     3721 gaagccggcg tgctcgtaca cggcgatggc ccgatcgttg tcggggaaga cgcccagcgc
     3781 caccttggcc aggccaccct cggccgccca ggccttggcc gcctccacca gcgccgagcc
     3841 gatccccacc tcgcgccagc catccttgac acagatcgag agcgtgccga catggtcgct
     3901 caccacgctg cgctcgacgc tgaccaggac gttgccgacg acgtcgccgt cggcttcggc
     3961 caccagcgcc aggctgctcg agtcgcggat catctcgccg atgtagaagg cctccgacgg
     4021 ctggctcacc gccgaggcgg gcgtcaccag ccagcgtccc tcgcggcgca cctccgcgaa
     4081 caggctcgcc agccctcgcg catcggtcgg gcgtgccggg cgcagcgtcc acggccggcc
     4141 atccttggcc gtccgcggct gggcctcggt gatcgtcatg ccggccactt gatcctcatc
     4201 ggccttcctt gctcgggtac gacaaagccc ccgcctcgtc agtgacgaag gggggccttc
     4261 gcggtaccac tctgacttga ccgattgggg tggtggagct ggggggactc gaacccccga
     4321 ccccctgcat gccatgcagg tgctctccca gctgagctac aaccccacga tcggtccact
     4381 catcggcgct gctatcggac gccatcccgg ccggtgctgc cggctgctca gcgggtgagt
     4441 tcggggtccc tgcgatcgcc ggcttgcacc ctcccggctc gctgcggatc gaggctgacg
     4501 cctagtgctc ccgcgtcaac gcgttcgggc gggagtatag cgcagggttc ctggaatgcc
     4561 tcattgcaag gccagcgcgg cctccaggcg ggtgaggctg gcatcgaggt cgcgctcggc
     4621 ggcatcggtc accatccgct ccagcggcgc catgaagatg ttctcggcgc ggacctccga
     4681 cgcgagcgtg atctccgtgc cgccgtcgtg agccacgagg tcgagagtgg ccacgatccc
     4741 catgccgctc acgctgctgg cgagggcgag gcgtcgctcc ggctcagctt cgcgcacggt
     4801 gatctcggcg gtcacctgct ggcccatcag ctccagcacg acccgcgccg tggagccgac
     4861 cgcagtgggc ggtggagagg tgcgctcggc ggccacgatg ccagcctgcc agcgcggcag
     4921 gttggccggc tcggccagga acgcgtacac ctcggccggc gtggcggcca tctgtgcggt
     4981 ccgttgcacg cgacgcatgg aacctacggt agtcgaccgg gcgggcgcgg cgagtggccc
     5041 atgaccatcg gtctacgctg gtgcgcagcc gaagcggcgt atcctgctcg cgaactcatt
     5101 catttcagga ggggctggac gtgctccgaa gattcgcgcc gctcgtggtc gccatgcttg
     5161 ctgtgctggt tgcatgccag agcacgccgg ggatcaccga tcccgttgac atcatcggcc
     5221 agggcctcga cgccaccgcc aacctgaaga gcctccacgt gtcgctcgcg ttggatggga
     5281 ccttcaaggt acccaacggc ggtggctcgt tcggcctcaa tgcgaccaag ctcgaggccg
     5341 acatcgacct caaggcgaag agcctccgcg cgacattggc ggtgcctgcc ttcctcaacc
     5401 tgactgcgga cgcgatcgtc atcggccagg acgtgtggta caggacgagc ctgggcggca
     5461 ccaagtggtt ccaccaggcg aacgacgtga tgggcagccc ttctccgtcc gcatcactcg
     5521 atccagtcgc gatggtcgcc aaggtcaagg aggcacttgc gaccgaaggc gtcgtgacca
     5581 agaagctcgc cgacgttgcc tgcggtgacc gccagtgcta ccaagtcagc gtgaccgtgc
     5641 catcggccct gatgaagacg gccggggcgg cggcctcgat ggatccttcc tcggtcttcg
     5701 gcgacgcgat ggtgatcaac ctgctcttcg atcgcgagaa gctgtggctg accgaggtct
     5761 ccacgagcgt cgacagcacg acggtcggca ccatcaaggc cactctgagc ttcagcaact
     5821 tcgacgaggc ggtggacttc agtccgcctc cctccccgga cgttaccgaa ggggagttca
     5881 cgatcccggg gatgtgaccg tcggcgcgct ggagagatgg cctgaaccgc aacggtgact
     5941 agcttgcggc gtcgatcgcg cgctcaaggt cgcggaggcg tcgagcctcg gtggcaatga
     6001 cgttgatgca gttgcatgac ttctcagcca gcccctcatg gttggcgatg ctgatctggc
     6061 cccgcgcata gttgacgaga cccgcgtcct tgaactggcg catcaccacg gacaggccgg
     6121 gacgcctgac gccgatcatc tgggccagga attcctgcgt cacgtggaac ggcttttcgt
     6181 cgacgcgttc gtccacctgg agcagccagc gggccgctcg ctggtccagc cgatgcatgc
     6241 gattgcacag gatcagctgc gccatcgtca ccatcacgac ctgggcatgc cgctgcagca
     6301 ttcgcactag cgtcccccca cggcgcagct catccaccag cacctcggat cgcatccgga
     6361 cgcccgtcag cggcacctgc acgatggtgc gcacgggcga ctgcccggtg cccaggaaga
     6421 ccggcaggcc cgtcatcccc tcccgcccga tcatggcggt atccaccgac tcgccaccct
     6481 cctcgagcgc cacgatggag gccacgccgc tcgttgggaa gtagacgaac tcgatcctct
     6541 cgttgggcga ctccagttcc gcggagcgct cgaggcggac cggttcgagc tcggcatgga
     6601 tgcgtgcgaa ctcttcgtcc gtcaacccac gcagcaggtg attggctcga ggatcgacgg
     6661 tcggcgcgtc aggatgggag ttgcttcgca tgacgaccta ttgtctcctt gtcgtatgtc
     6721 caaaagcgta cagacgacat atgagacatt ggcatcctcg ggctgacggc gaatgtgtca
     6781 gaccgattgc agaacttgtt ccgtgaggtg accagccaaa tgccgtcgac caccggtcac
     6841 gccgctgagg cagtgctcag cctggacgca acgggccggt acgtcggtgc caataagtcc
     6901 gcgctcgagc tcctcggtgt cacgc
//
LOCUS       DATCQR010000111         5276 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_98862, whole genome shotgun sequence.
ACCESSION   DATCQR010000111 DATCQR010000000
VERSION     DATCQR010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5276
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98862"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            165..1709
                     /locus_tag="VIN32_03710"
     CDS             165..1709
                     /locus_tag="VIN32_03710"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8921719.1"
                     /translation="MRLSAWGILAVASLGVFLGGAELMVVVIALPSIVADFGGWADLG
                     RASWIVNGYLLAYIVAMPLAGRAADLWGARRLYLLALTLFCLGSLGAGLSRIAGPEAG
                     LTWLIGSRVVQGLGGGALVPLSMALASHLFAGRARSIALGVEGAATFVGMAVGPAYGA
                     WVLLNVALPIPGLDVVGWQWIFFLNVPVGLVVLLLLYVVAGGVETPRARGRLDLAAGL
                     LASLALVAGVGALSLAGERGWDDPLVLGGLAASALCLGIFVVRELRQAAPLVDPRLFA
                     DARFAASNGVSLLTGYTLATAIIGGPVFVNRVLNAGADRSAAALTALTLAIAIGAVAG
                     GLGSGLVGQRLTAVVGVGLTIIGLWFATGWGVDTDIGRLQRDLAIFGAGFGLTVSPRA
                     TAAVEAAGASAYGVASAMLQVTRTIGMSVGLALLTSIGQNRIDELTALINDPPHRDAL
                     VTTLGHPEFAGVDPRSSLALVDLLETWSRGEAADVLRLVFQIALAVAALTLIPALLVE
                     GRRRAR"
     gene            complement(1706..2383)
                     /locus_tag="VIN32_03715"
     CDS             complement(1706..2383)
                     /locus_tag="VIN32_03715"
                     /inference="COORDINATES: protein motif:HMM:NF016119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HEY8921720.1"
                     /translation="MKASSAPGIVLPLLLAVIGASLIVAGRLPVDKPGTSLPGIPDQS
                     EVAVATPTPGPSVFTPEPTPSPTPIPDSWVAVQLEVPSVELNVAVRHAVPGQCDFPPE
                     DGAYVLCQSSEPGRGANSYIVGHALQHLLKRLWNVVLGAEVKVLMSDGVVLHYVVTEI
                     HPNVSCPDSRADPMPNPPLALQYARPGCPGAEWSRKTDHERLTLQTSQGFNRNWGELV
                     VIAEPLF"
     gene            complement(2409..3860)
                     /locus_tag="VIN32_03720"
     CDS             complement(2409..3860)
                     /locus_tag="VIN32_03720"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HEY8921721.1"
                     /translation="MVDDLSAILPELALTALVLAVLTTDLFLRHEDKWLLTPITVIGL
                     GFVGYACWTVWGVNQTVFAGFYRVDDLSVFFKVATIVIGILAALFAPSYLAQRKLPLG
                     EFNVILLFSLSGMFVLASSSDLITLFIGLELMVMPAYLLAGFHKTDRYSNEGGLKYFL
                     LGSFASAILLFGISWTYGLTHSTRLSEIGGLLTDGGAAGLIAIAMLTVGSTFKAAAVP
                     FHYWTPDAYQGAPTPITGFLSVGPKLGAFALLVRVFGEGLGLSGADWYGVMAILAVLT
                     MTGGNIVALAQTNVKRMLAYSSIAHTGYILAGLAAFAHATDPAVRTQGIEAILFYVLG
                     YGVMNIAAFSVVGMLQRDPRRFGGLSAFAGLAGRAPALAAAMAVLLLSLTGIPPTVGF
                     FAKLYVLVATVDAGLAWLAVAIALNAALAAFYYLRVIVYMYMREPEADPAPIDRSPLT
                     AFALAISVAGVLLLGLFPDPIISFLRASAAGLL"
     gene            complement(3854..>5276)
                     /locus_tag="VIN32_03725"
     CDS             complement(3854..>5276)
                     /locus_tag="VIN32_03725"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEY8921722.1"
                     /translation="LISLIVFGLSLLLVLGFKTGADGFQFEERASWIPAFGINYHLGV
                     DGISLILVLLTTTLTWISILASFSPIQDRVKEYMISFLVLEVGMLGVFLALDLFLFYI
                     FWEVVLVPMYLIIGIWGGPNRIYATIKFVLYTLIGSLLMLVAILATAFSYQAATGSWN
                     GAFDYLTLRGFGFDGTLQILAFLAFFLAFAIKVPMWPFHTWLPDAHVEAPTAGSIILA
                     GVLLKLGGYGFLRYSLPLAPDAAVAFAPIIIGLAVVAVLYGAMVSMVQPDLKKLIAYS
                     SVSHMGFVMLGVFVFNTQGLQGAVFQMISHGVTTGALFLLVGVIYERTHDRQIAHMGG
                     LNGRLPRYAAIFGLFTFASIGLPGLSGFIGEFLVVLGAFQYSGWVAAATMLVVIISAV
                     YMLWMFQRVFFAVPSDWMRRFWPTLTDMTRNEWLALTPLILLVVGLGVLPGPVLDAIS
                     APVQRILDVVNASPGLTSWSLPW"
BASE COUNT          853 a         1729 c         1919 g          775 t
ORIGIN      
        1 ccgctgagct cgcttccgct cccggtcggg ggcctcggcc tgctcaccca cattgagtcc
       61 gccgttcgcc cggtggccgg agcgctccag tcggcggggg cagctctggc ggcggcggcc
      121 gttctgctgg tcctggccgg ggcggggctc gagcgcagcg atctgtgagg ctgtccgcct
      181 gggggatcct ggcggtggcc tccctcggtg tcttcctcgg aggcgcggag ctgatggtgg
      241 tggtcatcgc gctcccctcg atcgtggccg acttcggcgg ctgggccgat ctgggccgcg
      301 ccagctggat cgtcaacggc tacctgctgg cctacatcgt ggccatgccc ctggccgggc
      361 gagccgccga cctgtggggc gcgcgccggc tctacctgct tgccctgacg ctcttctgcc
      421 tcggcagcct gggtgccggg ctgtcgcgta tcgcgggccc tgaggcgggc ctgacctggc
      481 tgatcggttc gcgcgtggtg cagggcctgg gcggcggagc cctcgtcccg ctctcgatgg
      541 cgctggccag ccatctattc gccggcaggg cgcgatcaat cgcgctgggg gtcgaggggg
      601 cggcaacatt cgtgggcatg gcggttggcc cggcctacgg cgcgtgggtc ctgctcaacg
      661 tggcgctccc tatccctggg ctcgacgtgg tcggctggca gtggatcttc ttcctgaacg
      721 tgccggtcgg cctggtggtc ctgctcctgc tgtacgtggt ggccggggga gtcgagacgc
      781 cccgagcccg cggtcggctg gacctcgcgg cggggctgct cgcatcactg gcgctggtgg
      841 ccggggtggg agccctatcg ctcgccggcg agcggggctg ggacgacccg ctggtgctcg
      901 gcgggttggc ggcctcggcg ctgtgcctgg gcatcttcgt ggtgcgcgag ctgcgccagg
      961 cggcgcccct cgtcgacccg cgcctcttcg ccgacgcacg cttcgcggca tcgaatgggg
     1021 tgagcctgct caccggctac accctggcca cggccatcat cggcgggccg gtcttcgtca
     1081 accgcgtgct caatgccggc gccgaccgat ccgcggcagc gctcaccgcc ctgaccctcg
     1141 ccatcgcgat cggtgcagtg gccggtgggc tgggatccgg cctggtcggc cagcggctca
     1201 cggccgtggt cggggtgggg ctgaccatca tcggcctctg gttcgccacg ggctgggggg
     1261 tcgataccga catcgggcgg ctgcagcggg acctggccat cttcggggcc ggcttcgggc
     1321 tgaccgtctc gccgcgggcc accgcggctg tcgaggcggc cggggcatcg gcctatggag
     1381 tggcgagcgc gatgctgcag gtgacgcgca cgatcggcat gagcgtgggc ctggcgctgc
     1441 tgaccagcat cggccagaac cggatcgacg agctcacggc cctcatcaat gacccgccgc
     1501 accgcgacgc gctggtcacc acgcttgggc accccgagtt cgcgggcgtc gacccgcggt
     1561 cgagcctggc gctggtcgat ctgctggaga cctggtcgcg tggagaggcc gccgacgtcc
     1621 tgcgcctcgt cttccagatc gccttggcgg tggcggcgct gaccctgatc ccggccctgc
     1681 tggtggaggg tcggcgccgc gcgcgctaga agagcggctc ggcgatgacg accagctcgc
     1741 cccagttgcg gttgaatccc tggctggtct gcagcgtcag ccgctcgtgg tcggtcttgc
     1801 gtgaccactc ggcgccgggg cagccaggcc tggcgtattg cagcgccagc ggcgggtttg
     1861 gcatgggatc cgcccggctg tccgggcagc tgacgttcgg gtggatctcg gtcacgacgt
     1921 agtgcagcac gaccccgtcg gacatgagga ccttgacctc ggcgcccagc acgacgttcc
     1981 agagtcgctt caagaggtgc tgcagcgcgt ggccgacgat gtagctgttc gcgccgcgcc
     2041 ccggctctga gctctggcac aggacgtagg ccccgtcctc tggcgggaag tcgcactgcc
     2101 cgggcacggc gtgcctgacc gccacgttca actcgaccga gggcacctcc agctggaccg
     2161 ccacccagct gtcgggaatg ggggtgggcg acggggtggg ctctggcgtg aagactgagg
     2221 gcccgggcgt cggggtggcc accgcgacct cgctctggtc cgggatcccg ggcagactgg
     2281 ttccgggctt atcgaccggc agccgcccgg ccacgatgag ggaggcgccg atcacggcca
     2341 ggagcagcgg cagcacgatt cccggcgcgg aggaggcctt catcggcccg agtctagccg
     2401 ctcgtcggtc agagcagtcc ggcagccgag gcccgcagga agctgatgat cgggtccggg
     2461 aagaggccca gcagcagcac gcctgccacc gagatggcga gcgcgaaggc ggtgagcggc
     2521 gagcggtcga tgggcgccgg atcggcctcc ggctcgcgca tgtacatgta cacgatcacg
     2581 cgcaggtagt agaaggccgc cagcgcggca ttcagggcga tcgccacggc cagccacgcg
     2641 agacccgcat caacggtcgc gacgaggacg tacagcttgg cgaagaagcc caccgtcggc
     2701 gggatcccgg tcagggagag gagcaggacg gccatcgccg cggccagggc aggcgcgcgt
     2761 cccgcgagcc ccgcgaaggc cgacagtccg ccgaacctgc gcgggtcgcg ctgcagcatg
     2821 ccgacgacgc tgaaggccgc gatgttcatc accccgtagc cgaggacgta gaagaggatc
     2881 gcctcgatcc cctgggtccg gacggcgggg tcggtggcgt gggcgaacgc cgcaaggccg
     2941 gcgaggatat agccggtgtg ggcgatgctc gagtaggcga gcatccgctt cacgttcgtc
     3001 tgcgccagtg ccacgatgtt gccgccggtc atggtcagca ccgccaggat cgccatcacc
     3061 ccgtaccagt cggcgccgct cagccccagt ccctcaccga agacgcggac caggagcgcg
     3121 aaggcgccga gcttgggccc gaccgacagg aagccggtga tcggggtcgg tgcgccctgg
     3181 taggcgtccg gggtccagta gtggaacggc acggcggcgg ccttgaaggt cgagccgacg
     3241 gtcagcatgg cgatcgcgat caggccggca gcaccgccat cggtcagcag gccaccgatc
     3301 tccgacaggc gggtcgagtg ggtcaggcca taggtccagc tgatcccgaa caggaggatc
     3361 gcgctggcga agctgccgag caggaagtac ttcagcccgc cctcgttgct gtatcggtcc
     3421 gtcttgtgga agcccgccag caggtaggcc ggcatcacca tcagctccag gccgatgaag
     3481 agggtgatca ggtcgctcga cgaggcgagg acgaacatcc cgcttaggct gaagagcagg
     3541 atcacgttga actcgccgag cggcagcttc cgctgcgcca ggtaggaggg cgcgaagagt
     3601 gcggccagga tgccgatcac gatggtggcg accttgaaga agaccgagag gtcgtcgacg
     3661 cggtagaagc cggcgaagac tgtctggttg acgccccaca cggtccagca ggcataaccc
     3721 acgaagccca ggccgatcac cgtgatcggc gtcagcagcc acttgtcctc gtgccgcagg
     3781 aagaggtcgg tggtgagcac cgccaggacc agcgcagtca gcgccagctc cggcaggatc
     3841 gcggacaggt catctaccac ggcaggctcc agctggtgag cccgggcgac gcgttcacga
     3901 cgtccaggat ccgctgcacc ggcgccgaga tcgcgtcgag gacgggaccg ggcaggacgc
     3961 caagcccgac cacgaggagg atgagcgggg tcagcgccag ccattcattg cgagtcatgt
     4021 cggtcagggt tggccagaag cgacgcatcc agtccgacgg gacggcgaag aagacgcgct
     4081 ggaacatcca cagcatgtag accgcactga tgatcactac cagcatcgtg gcggcggcga
     4141 cccagccgga gtactggaag gcgcccagca cgaccaggaa ctcaccgatg aaccccgaca
     4201 gcccgggcag cccgatgctg gcgaaggtga acagcccgaa gatcgccgcg tagcgcggca
     4261 ggcggccgtt gagcccgccc atgtgcgcga tctggcggtc gtgtgtccgc tcgtagatga
     4321 cgccgaccag caggaagagt gctcccgtcg tcaccccgtg gctgatcatc tggaacacgg
     4381 cgccctgcaa cccctgcgta ttgaagacga agacgcccag catcacgaag cccatgtggc
     4441 tgaccgatga gtaggcgatc agcttcttga ggtccggctg caccatgctg accatggccc
     4501 cgtacaggac ggccaccacc gccaggccga tgatgatcgg ggcgaaggcg accgccgcgt
     4561 cgggggccag cggcagcgag tagcgcagga agccgtagcc gcccagcttc aggagcaccc
     4621 ccgccaggat gatgctgccg gcggtcggcg cctcgacgtg cgcatcgggg agccaggtgt
     4681 ggaacggcca catcgggacc ttgatggcga aggcaaggaa gaaggcaagg aacgccagga
     4741 tctggagcgt gccgtcgaag ccgaagccgc gcagcgtcag gtagtcgaag gcgccgttcc
     4801 agctgccggt cgcggcctgg taggaaaagg cggtggcaag gatcgccacc agcatcagca
     4861 gtgagccgat gagcgtgtac aggacgaact tgatcgttgc gtagatgcgg ttgggcccgc
     4921 cccagatgcc gatgatcagg tacatcggca ccagcacgac ttcccagaag atgtagaaca
     4981 ggaagaggtc gagcgcaagg aagacgccga gcatgccgac ctcgagcacg aggaaggaga
     5041 tcatgtactc cttcacccgg tcctggatcg gcgagaagct ggccaggatg ctgatccacg
     5101 tcagcgtggt cgtgagcagc accaggatca gggagatgcc gtcgacgcca aggtggtagt
     5161 tgatcccgaa cgccgggatc cacgacgcgc gctcctcgaa ctggaagccg tcggcgccgg
     5221 tcttgaagcc gagcaccagc agcagcgaca gcccgaacac gatcagcgag atgagc
//
LOCUS       DATCQR010000112         9880 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_69023, whole genome shotgun sequence.
ACCESSION   DATCQR010000112 DATCQR010000000
VERSION     DATCQR010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 9880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9880
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69023"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..414
                     /locus_tag="VIN32_03730"
     CDS             <1..414
                     /locus_tag="VIN32_03730"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEY8921723.1"
                     /translation="VRARLATARVALLVLSRDEAAALLDRPLPSLAAAADAVAGALPG
                     LIAVVTGGAAGSSAAGPGFALSVPAVEQTTPMLDATGAGDAYLAALILFLLEATWPPA
                     VPTLREAMERGARDGALAARVLGAQGRTAAESTTR"
     gene            411..1346
                     /locus_tag="VIN32_03735"
     CDS             411..1346
                     /locus_tag="VIN32_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008877741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HEY8921724.1"
                     /translation="MSASTLLSISDEVAEAIATGGAVVALESSLIAQGLPSPQNLETA
                     QACESVVRQEGAVPATTAIDAGKLLVGVPPAVLERLADPGNDPAKAGSGDLAPVLASG
                     GLASTTVSAAVRIAAMAGIRVLATGGIGGVHRGAATTFDVSSDIDELAATRVAVVCSG
                     AKSILDLPATLELLETRRVPVIGLGVDELPAFYSAGSGLRLAHRVEGPAEAAAVLALH
                     LAIDGSHGILFVQPPPADLAIPSNEVASWLDLANADLARQGIRGGAVTPFLLGRVAQL
                     SDGRALRTNIGLIVNNARTAARLAVALARMRTGIA"
     gene            1419..1958
                     /locus_tag="VIN32_03740"
     CDS             1419..1958
                     /locus_tag="VIN32_03740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921725.1"
                     /translation="MRIYEGSPRQDWEEVLRSIGAFVDRERIKELLLLELDAGFILQG
                     MAMPAAGARTESEMTLVKITRELPDDEVAELMEVAGAKRSDAPPGTPHTEIANYYEHA
                     MRVIGAYLDLQHPRDLFFFEQGGAFVLRLLMFGAGAAPTGHQLVEFTKDDIMSMIEAA
                     PMQRNKPAPESGPPGETVA"
     gene            1955..2107
                     /locus_tag="VIN32_03745"
     CDS             1955..2107
                     /locus_tag="VIN32_03745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921726.1"
                     /translation="MKLFKILFVTIGALLAAMIIIPLTVLAGLFVWLKLTEEGDEDDL
                     EIEDAV"
     gene            complement(2104..2913)
                     /locus_tag="VIN32_03750"
     CDS             complement(2104..2913)
                     /locus_tag="VIN32_03750"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HEY8921727.1"
                     /translation="MPAAIRLIHPAPALAVVALSAALGAILATQAGVPIGERWLFTVL
                     AVAGSQVFTGATNDIADRFRDATVRPEKPLPAGELSLNAATWIASIGLAVQLAASWRT
                     GALPLVLGAAASSSALAYNLLLSRTPLSPLAYLLSFGLLPAWIAAGVGIPVERVLPAI
                     PLVAPFAMAAHLANTLRDFEADAAGSSRSLAQVLGRRASRLLAAGLALGVGLGVGAAL
                     LIGGRASPASVALGLIGLVAIAGGTTSERRLWYGILLAAVCWTAAWALSTG"
     gene            3023..4723
                     /locus_tag="VIN32_03755"
     CDS             3023..4723
                     /locus_tag="VIN32_03755"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEY8921728.1"
                     /translation="MPKYVVVTGGVTSSLGKGITAASIGRILKARGIPVSILKLDPYI
                     NIDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDENLSQASNVTTGRIYQSVIAKE
                     RRGDYLGGTVQVIPHITNEIKERIARVAREGTGSVATSGLASHGVVVVEVGGTVGDIE
                     SLPFLEALRQMRKDVGRRNVLYVHVTWLPQIGATGELKTKPTQHSVKELRGIGIQPDV
                     IILRSDEPIDDEILDKVSLFTDVDVNAVIPLKTANSIYEVPIQLERAGLGKLITRELE
                     IPGSEPDLRDWQELVARIRAPRPEVEIAIVGKYTELPDAYISVSEALKHAALHHRVDV
                     KVRWIRSEHLERIEQEQVDDELKGVAGLLVPGGFGYRGVEGKIRAIRWAREQRVPYLG
                     LCLGLQCAVIEFARMALGTEDANSSEFNVFTEHPVIDLMPDQTDVTDKGGTMRLGLYP
                     ARLEESSKVREAYGTEIAYERHRHRFEVNNAYRERLSEAGMWFSGVSPDDRLVEYIEL
                     RDHPWFVATQAHPELKSRPSRPHPLFRDFVGAAAARLGLGATTDTAEAAVIPVAEGNP
                     "
     gene            4728..6302
                     /locus_tag="VIN32_03760"
     CDS             4728..6302
                     /locus_tag="VIN32_03760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921729.1"
                     /translation="MSPRAVAVRKTAFDRSAYERDLGAFIAEREEELYQHGAGLHDEL
                     ALGPIYERHALLFSRPVVDALRRLLEAGGPETGHNRALLAAATNGFIERATAELTDAI
                     GTAEATAVVMWRGESIPYRGLRNRISEISGRAERNGLYASYLEAEEALNPLRRQRFDR
                     IGGLARELGYTDYVELIKVTRGFDPDELAVGLRGFLDESETPYFAALRRYLARIEIEQ
                     GDASLADLWFLMRGSGWDNWFDGHRILTILGSTLRGLGIELRDQPGATLDVEVRPNKS
                     PRAFVAVVNAPRDVRLVIQPRGGWDDYGAVLHEAGHLEHFLHVDPDLPVSLRELGDAS
                     VTEGYAMTFERLLAEPEWLVTEIGMPAEHAASFADFFSLYHLVLLRSLAARHLHELRM
                     HRGGDEVVHRATYAGLAGLLTGVREPEERYLVIDDGMYAASYLRCSMLDAALTDYLIT
                     RHGETWWRSAAAGDTLKRAWNRGQEWTAEQVVAHLGYDSLDWRPVVRQIRAKLVGEMS
                     GYGGPNITTRAGTRKV"
     gene            6427..7899
                     /gene="rho"
                     /locus_tag="VIN32_03765"
     CDS             6427..7899
                     /gene="rho"
                     /locus_tag="VIN32_03765"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEY8921730.1"
                     /translation="MNDETRSQRRGRPRRRPDGGGPRRQAPRPVYQGRRNNYATGGYS
                     SIFAGPFPREGDETDEGWNLAELQARSLEELRELGSELGVEGADELEHSDLVLKLLDV
                     VPLTPAQPRLDQNGQPGIATGVLEIVDEGFAFLRRHGVMPSNEDIYVSSSQVRRFGLR
                     TGDKVEGQTRPPKDQEKYWGLLRVDTVNGVDPDTARRRPYFDTLVPVFPDEMFDIETD
                     QKNLTQRLINLISPIGKGQRGLILSPAKAGKTTVLKHIAAGITDNYPEAVLMVALIGE
                     RPEEVTDMSRSVKGEIFASTFDEPTENHTRVAEMCLEIAKRQVETGRDVVVLLDSITR
                     LARAYNLALPASGKTLSGGVDPIALYPPKRFFGAARNIEGGGSLTIIATCLVDTGSRM
                     DDVIYEEFKGTGNMELALDRKLSEKRIFPAIDVLRSGTRREELLLDENTLRMIWTMRR
                     MLSAVGPNEGIELLMQRLGKTTNNAEFLVSLSKSVEASER"
     gene            7914..9287
                     /locus_tag="VIN32_03770"
     CDS             7914..9287
                     /locus_tag="VIN32_03770"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEY8921731.1"
                     /translation="MLESARRQVAALLVGALLLAACGPAVPSPSVPQSAQPSASITAS
                     PGVGEIVPAPGSSSTVYEPNPNAIVVAIDPGHGGCLDWGVPDPRERGAAFSEKVMTLA
                     IGLELKRLLEEQGITVVMTRETDVALAGDDYPALGCNGPPFRDVNGDGETGFDPEGKT
                     RTHDELQARLDLINLARADLLVSIHINSLTQNGVVFEIAATQTYYTDETPWGVSATQH
                     LAQQVQNGVVGAMDRAASYDRQDRGISAVNFFVVAPPLFATTPDRPDPTTQPTRGALM
                     PAVLTEVGSITLAAEHDLLLSPVGQAAAAQGIATGLKAYFADRPLAVRFDALIPLGEA
                     GLMPTAVGGSGPPFWSTGVRRAELSIGVPLRMTNTGTEPWPDDLRVLAGWEASGEPYL
                     RQAPDALDDIGLDVPPLAPGESFVLVFRLDVPVAERGMLWVTLGNDVASLDDLGSAPL
                     QLSTEGP"
     gene            9468..>9880
                     /locus_tag="VIN32_03775"
     CDS             9468..>9880
                     /locus_tag="VIN32_03775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921732.1"
                     /translation="MPDERRPSRRAADERPTARAVSHLAVLALMAVTVIVGVQATTGA
                     STTGSAHRGFPFGLIAVAQGADAASLEQQAIRYELLPQPESQQPMPERGARAEVPVIT
                     PLPTPVAPPPPPAPPAPKAVYVRAAPVVGTGQLLWP"
BASE COUNT         1592 a         3463 c         3377 g         1448 t
ORIGIN      
        1 gtccgggcgc gcctggcgac cgctcgagtc gccctgctgg tcctgtcccg ggatgaggcg
       61 gcggcgctgc tcgatcggcc gctcccatcc ctggccgccg ctgcggatgc cgtggctggg
      121 gccttgcccg ggctgatcgc cgtcgtcaca ggtggcgctg cgggctcgtc ggcggcggga
      181 cctggcttcg ccctgtcggt gcctgcggtc gaacagacga cgccgatgct ggacgccacc
      241 ggggccggcg acgcgtacct cgcggccttg atcctgttcc tgctcgaggc aacatggcca
      301 cccgccgttc ccaccctccg cgaggcgatg gagcgtggtg cccgggacgg tgcccttgcc
      361 gcccgcgtgc tcggagcgca gggaaggacg gcagcggagt cgacgacccg atgagcgcgt
      421 cgacgcttct ctcgatctcg gacgaggtcg ctgaagcgat cgccacaggt ggtgcggtgg
      481 tggcgctcga gtcgagcctc atcgcgcagg gactgccgtc gccgcagaac ctcgagacgg
      541 cgcaggcgtg tgaaagcgtc gtgcggcagg agggggcggt gccggcgacg acggcgatcg
      601 acgccgggaa gttactggtt ggggtcccgc cggccgtgct cgagcgcctg gcggacccgg
      661 ggaatgatcc ggccaaggcc gggtcgggcg accttgcgcc ggttctcgcg tccgggggcc
      721 tggcatcgac caccgtcagc gccgccgttc ggatcgccgc catggccggc atacgcgtgc
      781 tggccaccgg ggggatcggc ggcgtgcatc gcggcgccgc cacgaccttt gacgtcagca
      841 gtgatatcga cgagctggcc gccactcgcg tcgccgtcgt gtgcagtggc gcgaagtcga
      901 tcctcgacct gcctgccacc ctggagctgc tggagacgcg acgcgtgccg gtcatcgggc
      961 tgggcgtcga cgagcttccc gccttctaca gcgccggctc ggggctccgc ctggcacatc
     1021 gggtggaggg ccccgctgag gctgccgcgg tgctggccct ccacctggcc atcgacgggt
     1081 cgcatggcat cctgttcgtc cagcctcccc ccgccgacct ggccatcccc tcgaacgagg
     1141 ttgcgtcctg gctcgacctc gccaatgcgg accttgcgcg gcaggggatt cgcggcggcg
     1201 cggtgacccc gttcctcctc ggccgggtcg cccagctgtc cgacggccgc gcgcttcgca
     1261 ccaacatcgg tctgatcgtg aacaacgcga ggacagccgc ccgactggcc gtcgccctcg
     1321 cccggatgcg gaccggcatc gcctgaggaa tcgcatacag cggctgctat cctgccagtc
     1381 agcccgcaac gggccgcgac acatcagagg gaggtcgcat gcggatctac gaggggagcc
     1441 cgcggcagga ctgggaagag gtgctccgca gcatcggcgc cttcgtggac cgcgagcgga
     1501 tcaaggagct gctgctgctg gagctcgacg cgggcttcat cctgcagggg atggcgatgc
     1561 cggcggcggg cgcgcggacc gaatccgaga tgaccctggt caagatcacg cgtgagctgc
     1621 cggacgacga ggtcgccgag ctgatggagg tggccggggc caagcgctcg gatgcgcccc
     1681 ccgggacgcc ccacaccgag atcgcgaact actacgagca cgcaatgcgc gtgatcggcg
     1741 cctacctcga cctgcagcac ccgcgcgacc tgttcttctt cgagcaggga ggagcgttcg
     1801 tcctgcgcct gctcatgttc ggggccggtg ccgcgcccac gggccatcag ctggtcgaat
     1861 tcaccaagga cgacatcatg tctatgatcg aggctgcacc aatgcagcga aacaagccgg
     1921 cccctgaatc cgggccacct ggggagaccg tggcatgaag ctcttcaaga tcctgttcgt
     1981 caccatcggg gccctcctgg ccgcgatgat catcatcccg ctcaccgtcc tggccggcct
     2041 cttcgtgtgg ctcaagctga ccgaggaggg cgacgaggac gacctcgaga tcgaggacgc
     2101 cgtctagccg gtactcagcg cccacgccgc cgtccagcag acggcggcca gcaggattcc
     2161 gtaccagagc ctgcgctcgc tggtggttcc gccggcgatg gcaaccaggc cgatcagccc
     2221 cagcgcaacg ctcgcgggcg aggctcgccc gccgatcagc agcgcagcac ccacgcccag
     2281 gccgacgccc agcgccagtc ccgccgccag caggcggctt gcccgcctgc ccagcacctg
     2341 cgcgaggctg cgcgaggagc cagcggcatc tgcctcgaag tctcgcagcg tgttggccag
     2401 gtgcgccgcc atcgcgaacg gcgcgaccag cgggatggcg ggcagcaccc gctcgacggg
     2461 gatcccgacc ccggctgcga tccaggccgg gagcaggccg aagctgagga ggtaggcgag
     2521 gggggagagc ggcgtccggg acaggagcag gttgtaggcg agggcggaac tcgacgcggc
     2581 cgctccgagc accagcggca gggcgccggt gcgccacgaa gcggccagct gcacggccag
     2641 cccgatcgag gcgatccagg tcgccgcatt caggctgagc tcacccgcag ggagcggctt
     2701 ctccggtcgg acggtcgcat cgcggaagcg gtcggcgatg tcgttggtcg ccccggtgaa
     2761 gacctgggag ccggcaaccg ccagcaccgt gaagagccag cgctcgccga tcggcacgcc
     2821 agcctgcgtg gcgaggatcg cgccaagtgc ggcactcagg gcgaccacgg cgagtgccgg
     2881 cgcggggtgg atgaggcgaa tcgcagcagg catcgatggc atcggtgcgc tagactgacc
     2941 tcccgagtac ggcgttggcg tccggtgctc gcgtgttcct tttcgaaggc gcgcttgagc
     3001 cacaggcaga ggagcccgag cgatgccgaa gtatgtcgtt gtgaccggcg gcgtgaccag
     3061 ctcgctcggc aagggcatca cggccgcctc gatcggccgc atcctcaagg cccgcggcat
     3121 cccggtctcg atcctcaagc tcgacccgta catcaacatc gaccccggca cgatgtcgcc
     3181 ctaccagcac ggcgaggtct tcgtcaccga cgacggtgcg gagacggacc tcgacctcgg
     3241 gcactacgag cgcttcatcg acgagaacct gtcgcaggcg agcaacgtca ccaccgggcg
     3301 gatctaccag agcgtcatcg ccaaggagcg ccgcggcgat tacctgggcg gcaccgtgca
     3361 ggtcatcccg cacatcacca atgagatcaa ggagcgaatc gctcgcgttg cgcgggaggg
     3421 caccggctcg gtggcgacca gcgggcttgc ctcgcacggg gtggtggtgg tcgaggtcgg
     3481 cggcacggtc ggcgacatcg agagcctccc cttcctggag gcgctccgcc agatgcgcaa
     3541 ggacgtcggc cgtcgcaacg tcctgtacgt gcacgtcacc tggcttccgc agatcggcgc
     3601 aaccggcgag ctgaagacga agccgaccca gcactcggtc aaggagctgc gcgggatcgg
     3661 gatccagccg gacgtcatca tcctgcgcag cgacgagccg atcgatgacg agatcctcga
     3721 caaggtctcc ctcttcaccg acgtcgacgt caatgcggtg atcccgctca agacggcgaa
     3781 cagcatctac gaggtgccga ttcagctcga gcgcgcgggg ctcggcaagc tcatcacccg
     3841 cgagctggag atccccggca gcgagccgga cctgcgcgac tggcaggagc tggtggctcg
     3901 catccgcgct ccgcgtcccg aggtcgagat cgcgatcgtc ggcaagtaca ccgagcttcc
     3961 ggatgcctac atcagcgtct ccgaggcgct caagcacgcc gccctgcatc accgcgtcga
     4021 cgtgaaggtg cgctggatcc gcagcgagca cctggagcgg atcgagcagg agcaggtcga
     4081 tgacgagctg aaaggggttg ccgggctgct tgtcccgggc ggcttcgggt accgcggcgt
     4141 ggagggcaag atccgcgcaa tccgctgggc gcgcgagcag cgcgtcccgt atctggggct
     4201 gtgcctggga ctgcaatgcg ccgtgatcga attcgcccgg atggcgctcg gcaccgaaga
     4261 tgccaacagc tcggagttca acgtcttcac cgagcacccg gtcatcgacc tgatgccgga
     4321 ccagaccgat gtgaccgaca agggcgggac gatgcgactc ggcctctatc cagcccgcct
     4381 cgaggaatcc tcgaaggtgc gcgaggccta tggcacggag atcgcatacg agcgtcaccg
     4441 gcatcggttc gaggtcaaca atgcgtatcg cgaacgcctc tcggaggctg ggatgtggtt
     4501 cagcggcgtc tcgcccgacg atcgcctggt ggagtacatc gagctgcgcg accacccctg
     4561 gttcgtggcg acccaggcgc atccggagct gaagagcagg cccagccggc cacatccgct
     4621 cttccgcgac ttcgtcggcg ccgccgccgc gcgacttggc ctgggagcga ccacggatac
     4681 ggcggaggct gcggtgatcc cggtcgccga aggcaacccc tgagccgatg agcccgcgcg
     4741 ccgtcgccgt ccgcaagacg gccttcgacc ggtctgccta cgagcgcgac ctcggggcct
     4801 tcatcgccga gcgcgaggaa gagctgtacc agcacggcgc cggcctgcat gacgagttgg
     4861 ccctcgggcc gatctacgag cggcatgcgt tgctcttcag ccgtcccgtg gtggatgccc
     4921 tgcggcggct cctcgaggcc ggcggcccgg agacaggcca caaccgcgcc ctgctggccg
     4981 ctgccaccaa tgggttcatc gagcgagcca ccgccgagct gactgacgcc atcggcaccg
     5041 ccgaggcgac cgccgtggtc atgtggcgag gggagtcgat cccgtaccgg ggccttcgca
     5101 accgcatcag cgagatcagc ggccgcgcag aacggaatgg cctctacgcc tcatacctcg
     5161 aggcggaaga ggccctgaac ccgctccgtc ggcagcgatt cgatcggatc ggcggcctcg
     5221 cccgcgaact cggctacacc gactacgtcg agctgatcaa ggtcacccgc ggcttcgacc
     5281 ccgacgagct ggcggtcggg ctgcgtggct tcctggacga atcggagacg ccctatttcg
     5341 cggcgctgcg acggtacctg gcgcgcatcg agatcgagca gggtgatgcg agcctcgccg
     5401 acctgtggtt cctgatgcgc ggaagcgggt gggacaactg gttcgacggg catcggatcc
     5461 tgacgatcct cggttcgacg ctgcgcggcc tcggcatcga gctgcgcgac cagcccggcg
     5521 caacgctgga cgtggaggtg cgcccgaaca agtcgccgcg cgcgttcgtg gcggtcgtca
     5581 acgccccgcg cgacgttcgc ctcgtgatcc agccgcgcgg cgggtgggat gactacggcg
     5641 ccgtgctgca cgaggcaggg cacctggagc acttcctcca cgtcgatccc gacctgccgg
     5701 tgtcgctccg cgagctgggc gacgccagcg tgaccgaggg ctacgccatg accttcgagc
     5761 gactgctggc cgagcccgag tggcttgtca ccgagatcgg catgccggcc gagcacgccg
     5821 cctcctttgc cgacttcttc tcgctctatc acctcgtcct gctgcgctcg ctggccgcgc
     5881 gccacctgca tgagctgcgc atgcatcgag gtggcgacga ggtggtgcat cgggccacct
     5941 atgccgggct ggccgggctg ctgaccgggg tgcgggagcc ggaggagcgc tacctggtca
     6001 tcgacgacgg catgtatgcc gcttcctacc tgcgctgctc aatgctggac gccgcgctga
     6061 ccgattacct catcacccgc cacggcgaaa cctggtggcg ctccgcggcc gccggcgaca
     6121 cgctgaagcg agcgtggaat cggggacagg aatggactgc cgaacaggtg gttgcgcatc
     6181 tcgggtatga ttcgctcgac tggcgaccgg tcgtgcgtca gattcgggcc aagctcgtcg
     6241 gtgagatgag cggctacggc gggcccaaca tcaccactcg cgcagggacc cgcaaggttt
     6301 gaccggtcat ccgcccgcgc gtccccccgg acgagagcca cacgcttacg cgtgttcccg
     6361 cagaacctcg gccgaatgcg gcgctcgcta tccagattcc cttctgatcg aggttacagg
     6421 cacccaatga acgacgaaac ccgctcccag cgccgcggcc ggccacgacg cagacccgac
     6481 ggaggaggcc cgcgccgcca ggcgccgcgc cccgtctacc agggccggcg caacaactac
     6541 gcgaccggcg gctactcgtc gatcttcgct ggccccttcc cgcgcgaggg cgatgagacc
     6601 gatgagggct ggaacctcgc cgagctccag gcgcgcagcc tggaggagct ccgcgagctg
     6661 ggcagtgagc tgggcgtcga gggagccgac gagctggagc actccgacct cgtcctgaag
     6721 ctgctcgacg tggtgccgct cacccccgcg cagccacggc tcgaccagaa cgggcagccg
     6781 gggattgcca ccggagtcct cgagatcgtg gacgaggggt tcgcattcct gcgccgccac
     6841 ggcgtgatgc cctccaacga ggacatctac gtcagctcgt cgcaggtccg tcgcttcggc
     6901 ctgcggactg gagacaaggt cgaggggcag acccgcccac ccaaggacca ggagaagtac
     6961 tggggtctgc tgcgggtcga caccgtcaac ggcgtggacc cggataccgc ccgccggcgc
     7021 ccgtacttcg acacgctggt gccggtcttc cccgacgaga tgttcgacat cgagaccgac
     7081 cagaagaacc tgacccagcg cctcatcaac ctgatcagcc cgatcggcaa ggggcagcgt
     7141 ggcctgatcc tgtccccggc caaggcgggc aagacgaccg tcctgaagca catcgcggcg
     7201 gggatcaccg acaactatcc cgaggcggtc ctgatggtcg ccctcatcgg cgagcggccg
     7261 gaagaggtca ccgacatgag tcggagcgtg aagggcgaga tcttcgcgtc caccttcgac
     7321 gagccgaccg agaaccacac gcgcgtcgcc gagatgtgcc tcgagatcgc caagcgccag
     7381 gtagagacgg gccgtgacgt cgtggtcctc ctcgactcga tcacccgcct ggcgcgcgcc
     7441 tacaacctgg ccctgccggc atcgggcaag acgctgtcag gtggcgtgga tccgatcgcc
     7501 ctctacccgc ccaagcgctt cttcggcgca gcgcgaaaca tcgagggcgg cggcagcctg
     7561 acgatcatcg ccacctgcct ggtcgacacg ggctcgcgca tggatgacgt catctacgag
     7621 gagttcaagg ggaccggcaa catggagctg gctctcgacc gcaagctgtc ggagaagcgc
     7681 atcttccccg ccatcgacgt gctccgctcc ggcactcgcc gcgaggagct gctgctcgac
     7741 gagaacaccc tgcgcatgat ctggacgatg cgacgaatgc tctccgcggt cggcccgaac
     7801 gaggggatcg agctgctgat gcagcggctc ggcaagacga cgaacaacgc cgagttcctc
     7861 gtctccctca gcaagagcgt cgaagcctcc gagcgctgac ccggccgcgt cccatgctcg
     7921 agtccgcgcg tcgtcaggtc gccgcgctgc tggtgggcgc ccttctgctt gccgcctgcg
     7981 ggccagccgt tccgagcccc agcgtcccgc aatcggcgca accgtcagcg tcgatcaccg
     8041 cgagccccgg cgtcggtgag atcgtgccgg cgcccggctc gtcaagcacg gtctacgagc
     8101 cgaatcccaa cgccatcgtg gtggcgatcg acccaggcca cggcggctgc ctcgactggg
     8161 gcgtgccgga cccgcgggag cgaggtgccg cgttcagcga gaaggtgatg accctcgcca
     8221 tcggcctcga gctgaagcgc ctgctcgagg agcagggcat cacggtggtc atgaccagag
     8281 agaccgatgt ggccctcgcc ggtgacgact atcccgccct gggctgcaac gggccgccgt
     8341 tccgcgacgt caatggcgac ggggagactg gcttcgatcc agagggcaag acacgcaccc
     8401 acgacgagct gcaggcgcgc ctggacctca tcaaccttgc gcgcgccgac ctgctggtca
     8461 gcatccacat caactcgctc acccagaacg gcgtcgtctt cgagatcgcg gccacccaga
     8521 cctactacac cgatgagacc ccatgggggg tgagcgccac gcagcacctc gcgcagcagg
     8581 tgcagaacgg cgtggttggg gcgatggacc gtgccgcgtc gtatgaccgc caggaccgtg
     8641 gcatctccgc cgtgaacttc ttcgtcgtcg cgccgccact cttcgcgacg acccccgatc
     8701 gtccggatcc caccacgcag ccgacgcgtg gcgcgctcat gcccgcggtg ctgaccgagg
     8761 tcggctccat caccctggcc gccgagcatg acctgctgct gtcacccgtc gggcaggctg
     8821 cggcagcgca gggaatcgcc accggcctca aggcgtactt cgccgatcgg ccgctcgcgg
     8881 tgcgcttcga cgcgctcatt ccgctcggcg aggcagggct catgccaacc gccgtcggtg
     8941 gcagcggacc gccgttctgg agcacgggag tgcgccgtgc cgagctgtcg atcggggtcc
     9001 cgctgcggat gacgaacacc gggaccgagc cctggccgga cgaccttcga gtgctcgccg
     9061 gctgggaggc gagcggcgag ccgtatctcc gccaggcccc cgatgcgctc gacgacatcg
     9121 gccttgatgt gcctccgctt gcgccgggcg aatcgttcgt gctggtgttc aggcttgatg
     9181 tcccggtcgc ggagcgcggg atgctgtggg tcacgctggg caacgacgtg gcgtcgctcg
     9241 acgacctcgg atcggcccct ctccagctct ccacggaagg cccataggac tccgatagga
     9301 ctacaagcgg cagccgaatg gggcgggttg caaggttgac cgcgtcttcg cgccgctggt
     9361 accatcggtg cgcacgactc tcggccggtg tcgtcgcccg tgccggcggg aagagcgggg
     9421 tcgggcaacc gacagaacca aggaatgcaa ccgcgggagc ttctgtattg cccgatgagc
     9481 gacggccttc gcgccgcgcg gccgacgagc gaccgaccgc acgagcggtc tcgcatctcg
     9541 cggtgctcgc cctcatggcg gtgaccgtca tcgtcggtgt gcaggccacg accggagcct
     9601 caaccaccgg cagcgctcac cgcggcttcc cgttcggcct catcgccgtg gcgcagggag
     9661 cggacgccgc gtcccttgag cagcaggcca tccgatacga gctcctccca cagcccgaga
     9721 gccagcagcc gatgccagag cgcggggcac gcgccgaagt gccggtcatc acaccgcttc
     9781 ccacgccggt tgccccaccg ccaccaccag ctccgcccgc tccgaaggcc gtctacgtga
     9841 gggccgcgcc agtcgtcggc accggccagc tgctgtggcc
//
LOCUS       DATCQR010000113         4252 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_84260, whole genome shotgun sequence.
ACCESSION   DATCQR010000113 DATCQR010000000
VERSION     DATCQR010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4252
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84260"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(87..2498)
                     /locus_tag="VIN32_03780"
     CDS             complement(87..2498)
                     /locus_tag="VIN32_03780"
                     /inference="COORDINATES: protein motif:HMM:TIGR01069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEY8921733.1"
                     /translation="MTRTDPVADPIAGGAADEGTLRALEFATIVEKLADLTAFSPSRE
                     LAEMALPLADAVHVGLLQDQTDEASRLLLEQSQATIGGARDIRQALGRARRGGRLAPT
                     DLLEIAETLRATSAFAARLIDWRGTHLASVRDDLDDAPQLRARIERTVDEAGEILDSA
                     SADLAAFRKRLRTAQDRVRERLNTMLRSSTLAGVIGEAIVTMRGGRYVIPIRADAKGK
                     LKGIVHDQSASGATLYVEPLMVVELNNTWTQASLDAAREEERILEELSREVEAAAEAL
                     LASLAGLARADVWMARARLGLQQDAVRPQLDDSAELLSARHPSLGPGAVPIDLRIGER
                     FGYRALIVTGPNTGGKTVSLKTLGLLALMHQAGLRVPAAEGARLPVFARVMADIGDEQ
                     SIAQSLSTFSSHLRNVVRFVASAGPGTLVLLDEIGAGTDPTEGSALAMAIVERLLASG
                     AMVAATTHYAELKAFAQEHVLVTNASVAFDVATLRPTYRLEIGLPGKSQAFAIAQRLG
                     LPAEILEDARSRLAAEHVSMEETLAAIGRAEAERSVELDRAREERQAATSDRERARSG
                     VQRARGEAAQMLADARRVADEILSRAEREVEEVRRELTRQRNLRSPRRRASAATDAFD
                     ALTERTARTRSESAGTDGAPAAVGGDTAQPRVGLWGRSRTLGTAGRIVDVSGSTGRVT
                     METDGARVVIPGDDVEIVPEPIAGPAPRDEEMAELKRRAASRIAPRLDLRGERVEAAL
                     EQLGAYLDEALLAGLDEAVIVHGAGTGALRRSIREYLADHARVRATRPGRREEGGDGA
                     TVAEL"
     gene            complement(2473..3075)
                     /locus_tag="VIN32_03785"
     CDS             complement(2473..3075)
                     /locus_tag="VIN32_03785"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEY8921734.1"
                     /translation="MIAAASVAAIDDRAWFDRSAAEVAPELLGAFLIHDTPAGRVAGR
                     IVEVEAYLGPEDLAAHSSRGQTPRNEVMFGQPGHLYVYLVYGMHHCVNVVCGPDSKPE
                     AVLLRAAELTLGEGLARSRRGDVPAARLAAGPGNLASAFGIDRRLNGADLLVGPVRLQ
                     HGRQPEAIDRTPRIGVDYAGGWTDRPLRYLIHDDPHRSRR"
     gene            complement(3072..3641)
                     /locus_tag="VIN32_03790"
     CDS             complement(3072..3641)
                     /locus_tag="VIN32_03790"
                     /inference="COORDINATES: protein motif:HMM:NF014704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEY8921735.1"
                     /translation="MDFLTKLGPIDLFVVAALAAGVFAGFMQGLVRYALNIVAVIVAF
                     ILAGLLRDPFDNILGGWNVFGPELRLQLVFLILFLGFTIAGWFIVRIFWRGTRLPIAK
                     QLDEIGGAVLGFVFAALAMVMTLVVMDTFFRTAPDAAVNSAGLLKGFYEALDSSVLID
                     FFRATLIPTLGQAARPFVPSDIGEFLTKP"
     gene            complement(3677..>4252)
                     /locus_tag="VIN32_03795"
     CDS             complement(3677..>4252)
                     /locus_tag="VIN32_03795"
                     /inference="COORDINATES: protein motif:HMM:NF015129.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921736.1"
                     /translation="PQEADVGDLKGIVEALHAALGALQPAFQPETAAKRHAHLHPGRA
                     ARIVDPTGHPYGHLGEVAPAVAAAWDLPGRPVVAVINLPQLLALAPAEVRAALVPAAQ
                     PLDRDMAVVVDAGTPVGELLRILRANAGPSLVSSHLFDEYRGPQVGEGKVSYAVALRF
                     QPEVAGDEHTVERAMKRIRGALQHHLGAQIR"
BASE COUNT          628 a         1531 c         1487 g          606 t
ORIGIN      
        1 gcgccgacgc ccgcgccgac tccgactgcg ctgccgagcc cgatacctac cccgccgccg
       61 ccgcccgcat ccgcagctcc ggtcccctag agctcggcga cggtggctcc gtcgcccccc
      121 tcctcgcgcc ggcccggtcg cgtggcgcgc acgcgggcat ggtcggccag gtactcccgg
      181 atcgagcggc gcagcgcccc ggttcccgcg ccgtgcacga tgaccgcctc gtccaggccg
      241 gccaggagcg cctcgtcgag gtaggcgccc agctgctcga gcgccgcctc gacgcgctct
      301 ccccgcaggt cgagccgcgg agcgatgcgg ctggccgctc ggcgcttcag ctccgccatc
      361 tcctcgtcgc gcggggccgg ccctgcgatc ggctcgggca cgatctcgac atcgtctccg
      421 ggaatcacga cccgtgcccc atcggtctcc atggtcacgc gcccagtgct accgctgacg
      481 tcgacgatcc gccccgccgt gcccagcgtg cgactgcgac cccacaggcc gacgcgcggc
      541 tgggcagtgt cgcccccaac cgcggcgggc gctccatccg tccccgcgga ctctgagcgc
      601 gtccgtgccg tccgttcggt gagggcgtcg aatgcgtcgg tggcggccga cgctctgcgt
      661 cgcggcgacc gcaggttgcg ctggcgggtc agctcacgcc gcacctcctc gacctcgcgc
      721 tcggcacggg acaggatctc gtccgcgact cttcgagcgt cggccagcat ctgggccgcc
      781 tcgccccgag cccgttgcac gccgctgcgg gcacgctccc ggtcgctcgt ggccgcctgg
      841 cgctcttccc tggcgcggtc gagctccacg gaccgctcgg cctcggcccg gccgatcgcg
      901 gccagggtct cctccatgct cacgtgctcc gccgccaaac gcgaccgggc atcctccagg
      961 atctctgccg gcaggccgag gcgctgggcg atggcgaagg cctgcgactt gccgggcagg
     1021 ccgatctcca gccgataggt gggtcgcagc gtcgccacgt cgaacgcgac cgaggcattg
     1081 gtgaccagca catgctcctg cgcgaacgcc ttcagttcgg cgtagtgggt ggtggccgcg
     1141 accatggccc cggatgcaag cagccgctcg acgatggcca tcgccagggc ggatccctcc
     1201 gtggggtccg ttcctgcccc gatctcgtcg agcaggacga gggttcccgg gccggcggag
     1261 gccacgaagc gcaccacgtt gcgcaggtgg ctcgagaagg tggagagcga ctgggcgatg
     1321 ctctgctcgt cgccgatgtc cgccatcacc cgcgcgaaca cgggcaggcg cgcgccttcg
     1381 gcggcgggga cgcgaagccc ggcctggtgc atgagagcga ggaggccgag cgtcttgagc
     1441 gagaccgtct tcccgccggt gttcgggccg gtgacgatca gtgcacggta gccaaaccgc
     1501 tccccgatgc gcaggtcgat cggcactgcc cccggcccga gcgacgggtg acgtgcggaa
     1561 agcagttcgg cggagtcatc gagctgaggg cgcaccgcgt cctgctgcag gcccagtcgc
     1621 gcccgtgcca tccacacgtc ggcgcgcgcc agtcctgcca gcgacgcaag cagggcctcg
     1681 gcagccgcct cgacctcgcg ggacagctct tcgaggattc gctcctcctc ccgggcggcg
     1741 tcgagtgaag cctgggtcca ggtgttgttg agctccacga ccatcagggg ctcgacgtag
     1801 agggtcgcgc cggacgcgct ctggtcgtgg acgatgccct tgagcttccc tttcgcatcg
     1861 gcccggatcg ggatcacgta gcggccgccg cgcatggtca cgattgcctc gccgatgacc
     1921 ccggccaggg tgctggagcg cagcatcgtg ttcaatcgct cgcgcacgcg gtcctgcgcg
     1981 gtgcggagtc gcttccggaa cgcggcgagg tcggcggacg ccgaatcgag gatctcgccc
     2041 gcctcgtcga ccgtgcgctc gatccgggcg cgcagctgcg gcgcatcgtc caggtcgtcg
     2101 cgaaccgatg caaggtgcgt gccccgccaa tcgatgaggc gcgccgcaaa ggcggaggtg
     2161 gcgcgcaggg tctcggcgat ctcgagcagg tcggtcggcg ccaggcgccc accgcgacgg
     2221 gcgcgcccga gtgcctggcg gatgtcgcgg gcacctccga tggtcgcctg cgactgctcg
     2281 agcagaagcc ggctggcctc atcggtctgg tcctggagca gtccaacgtg caccgcatcc
     2341 gcgagcggca gggccatctc cgccagctca cgcgacggac tgaaggctgt caggtcggcg
     2401 agcttttcga cgatggtcgc aaactcgagc gcgcgcagtg tcccctcatc cgccgcgccc
     2461 cccgcgatgg ggtcagcgac gggatcggtg cgggtcatcg tggatcaggt agcggagggg
     2521 tcggtcggtc cagcctccgg cgtagtcgac gccgatgcgt ggggtgcgat caatggcctc
     2581 tggctgccgc ccgtgctgga gccgcaccgg ccccaccagc aggtcggccc cgttcagccg
     2641 gcgatcgatc ccgaacgcgg aggcgaggtt gccgggaccc gcggcaagcc tggcagccgg
     2701 aacatcacca cgccgggacc gggccagccc ctcgcccagc gtcagctccg cagcgcgcag
     2761 cagcaccgcc tcgggcttgg aatcgggacc gcacaccacg ttgacgcagt ggtgcatccc
     2821 atacacgaga tagacgtaca ggtgcccggg ctgcccgaac atgacctcgt tgcgtggcgt
     2881 ctgcccgcgg gatgagtgcg ccgccaggtc ctcgggaccc agataggcct cgacctccac
     2941 gatccgacct gcgacccggc cggcgggcgt gtcgtggatc aggaaggcac cgagcagctc
     3001 cggcgcgact tcggctgccg agcggtcgaa ccaggcgcgg tcgtcgatcg cggcgactga
     3061 ggccgcggcg atcacggctt cgtcaggaac tcgccgatgt cggacggcac gaatggccgc
     3121 gcggcctgcc ccagggttgg gatcagggtg gcgcggaaga agtcgatcag caccgatgag
     3181 tccagcgcct cgtagaagcc cttcaatagg cccgccgagt tgaccgcggc gtccggcgcc
     3241 gttcggaaga aggtgtccat caccaccagc gtcatgacca tcgcgagggc cgcgaacacg
     3301 aagccaagca ccgcgccgcc gatctcgtcg agctgctttg cgatcgggag acgggttccg
     3361 cgccagaaga tgcggacgat gaaccagccg gcgatcgtga atcccaggaa caggatcagg
     3421 aagaccagct ggagccggag ctcagggccg aatacgttcc agccgccgag gatgttgtcg
     3481 aatggatcgc gcagcagccc ggcgaggatg aacgccacga tcactgccac gatattgagc
     3541 gcgtagcgca ccagcccctg catgaagcca gcgaagacgc cagcggccag ggccgcaacg
     3601 acgaacaggt cgatcggtcc cagcttcgtc aggaaatcca tcggtgcctc cctcggggcc
     3661 gcaagcctag cagccctcag cggatctggg caccgaggtg atgctgcagc gcgccccgga
     3721 tgcgcttcat ggcgcgctcg accgtgtgct cgtcgccggc gacctccggc tggaagcgta
     3781 gagcgaccgc atacgagacc ttcccctctc cgacctgtgg tccgcggtac tcgtcgaaga
     3841 ggtgcgacga cacgagcgac ggacctgcat tggcgcgcag gatgcgcagc agctcaccga
     3901 ccggtgtccc cgcatccacc actactgcca tgtcgcggtc gagcggctgc gcagccggga
     3961 ccagggcggc gcgaacctcg gccggagcga gggcgagcag ctgtggcagg ttgatgacgg
     4021 ccacgactgg ccgcccgggc aggtcccacg cggccgccac cgccggggcg acctccccga
     4081 ggtgaccgta ggggtgtcca gttggatcga cgatgcgcgc cgcccgccca ggatggaggt
     4141 gagcatggcg cttggcggcc gtttcgggtt gaaaggcagg ttgcagtgcg cccagcgcgg
     4201 cgtgcagcgc ctcgacgatt cccttcaggt cgccgacgtc tgcctcctgg gg
//
LOCUS       DATCQR010000114         6854 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_37716, whole genome shotgun sequence.
ACCESSION   DATCQR010000114 DATCQR010000000
VERSION     DATCQR010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6854
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37716"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            70..846
                     /locus_tag="VIN32_03800"
     CDS             70..846
                     /locus_tag="VIN32_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8921737.1"
                     /translation="MVGSRRKRKTVMAVNDVTIRIRRGEIYGVLGANGSGKSTLIRLV
                     STLLTLDTGRVEVFGHDIERDEMAVKRVINRVSVDAAFFKKLSPFENLAYAARLYGME
                     PKAARVEAISILARLGIGEKRLGRPLEQMSRGMQQKVAIARALLTSPTLLLLDEPTTG
                     LDPRSKLDVQTFIEELRDTHDATIVLTTHDLAEADRLCDRIGIINDGRIVVEDTPEAL
                     KAKVAEERGLEPTMESVFMTYTGRSLDDDLGDNEEKDDED"
     gene            850..1650
                     /locus_tag="VIN32_03805"
     CDS             850..1650
                     /locus_tag="VIN32_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007910501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8921738.1"
                     /translation="MALMPRELKASFAFVERNFNLTKRYWGWEVAFLVYAVAGALAVS
                     LIGAGQGDTRLLMTLVIGAVFWNYLSVVFSFIAETVSWERWEGTLEYTFMAPVRRYAQ
                     MLGSTVYAVAFGFVHTVVVLVVLALFFELDLSRANFATAAVFMVLGSVSFIGIGIMTA
                     ILPLMYVERGAQMTFVLQSVLLLISGVYYSVDVLPGWMQVLSNFSPATYVLHGVRAGL
                     IDGQGITSLLGDVWPLLLMGIVFIPLGLWAFGRAERYAKRTGKLKRVG"
     gene            1653..2645
                     /locus_tag="VIN32_03810"
     CDS             1653..2645
                     /locus_tag="VIN32_03810"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8921739.1"
                     /translation="MPTEIPGLSLRVHPGGTEPAALMAPIVSAVDAAIGIDELTTAAD
                     LENWLSHATETFDPSQDVVLAEVDGALVAYASVGWVDTSDGLREFRVGGYVHPDWQGK
                     GIGRRLLAWQEERARTHPATATERPLVIGTWSADENVRKVRLFTHAGYEQVRFFFEMR
                     RPTLDEIQLPPMPEGLEIRPIAANRASQKQLWDADREAFEDHWGGFDASDTAFEAWLT
                     RPNHNPALWVAAWDGDEIAGAVTNTIFADENAAFGIARGWMETVFVRRAWRRRGLGAA
                     LVARALVRLREAGMTEAALGVDSDNPSGALALYERAGLEIHRRSAAYRKPMEAR"
     gene            2645..3631
                     /locus_tag="VIN32_03815"
     CDS             2645..3631
                     /locus_tag="VIN32_03815"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8921740.1"
                     /translation="MRSQLDIRMRPHRDSDLPEFVRVANAANAADGIDERTSQAGLAS
                     WLLTARANYDPAHDLAVATVGEEIAGYGWVDWVETTDGKREYRTRGHVDPRWRRQGVG
                     SAILAHNEARLLKIAAEHSDDLPRVYGAFAPDLRVGAVALLSGAGYLPVRYFFDMVRA
                     TLDEIEVPPLPEGMEMRPVTDVEGYRRLFAADSEAFMDHWGGFEYSEATFQQWLGEPT
                     FDPSLFVIAWDGDEIAGAVLNSIDAEENEALDRRRGLLEGVFVRRPWRRRGLAAALVG
                     RSLELLRERGMTSAWLGVDADNPTGALGVYERAGFEIHRRASAYHKPMEIAR"
     gene            3628..4617
                     /locus_tag="VIN32_03820"
     CDS             3628..4617
                     /locus_tag="VIN32_03820"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8921741.1"
                     /translation="MTITLPEAPAIPDLQFRAMRRPADDAEIARLVNAGMAATNVPHR
                     LSQAQVTNWLDHPSNLDLAADLLFAEVNGSVMAYTEGGWEQDNDGGRNYSVWGQVHPD
                     WQRRGLGTALLHWIEERQSRVAAGHPDLEKRLQSWASESETGRLALLERNGYRIVRYD
                     FEMERPNLDDIQPLPLPAGIELRPAREEDLRRHWETEVEVFRDHWGAIDDSEASFEQM
                     RSDPRRDIGLWIVAWRGDEIVGQVLNRIDREVNAELGMKRGWVSSVGVRGPWRRQGVA
                     RALVAESLRVLRDAGMTSAGLGVDAENANGALGVYEATGFRVVRTERVYRKPL"
     gene            complement(4614..5828)
                     /locus_tag="VIN32_03825"
     CDS             complement(4614..5828)
                     /locus_tag="VIN32_03825"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY8921742.1"
                     /translation="MSEPIAPPAPPIAVGAPTAAAPSVANPAPMKIGIVSPYGYPHPG
                     GVNEHVRHAYEAMRRMGHDAWIITSKYGKEREDEGRVIRLGTGYAIPANGSMGRVTLG
                     WRFKQQARDLLAEHHFDVLHFHEPFVPFLSPTVLDQSDTVNIATFHAFGGFSPSYWVG
                     KWFAGQLAAKLHGRIAVSGAARHFISRYFPGDYRIIPNGVDLDRFADAEPFEELRDGT
                     INILFVGRFEERKGLIHLLRAYHRLRKRHVDARLLIIGSGPKQREYRRFVGLRQIRDV
                     EFLGRVSDDAKARYFASADIFCSPATGQESFGVVLLEAMAAGVPIIASDIHGYKNVVQ
                     RGVQGLLVEPKNHRALAAALYRLSSDEELRHRMGEEGRAKAPEYSWDRVTEQIVDFYR
                     EIREGVATTSGR"
     gene            complement(5825..6577)
                     /locus_tag="VIN32_03830"
     CDS             complement(5825..6577)
                     /locus_tag="VIN32_03830"
                     /inference="COORDINATES: protein motif:HMM:NF024660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP-associated domain-containing protein"
                     /protein_id="HEY8921743.1"
                     /translation="MADARPRGGGERGRADMHLHTLYSDGVMEVQALLDHVERSTELD
                     LIAITDHERIDGALRAQELHAAGDNHFGLVVGEEITTRRGHLLALFLTQRIPPLRPLE
                     ETIERIHAQGGIAIAPHPMAPLTPSLGRRSLVRLHHDPDLGHRLDAIEMLNPSVAGRA
                     RRPHRRALNQLMELAAVGNSDAHVLEHVGTGWTWFAGASAEDYRRAIADRTTEAQGEY
                     WTHWHNVSVYGRQLVAKARHVRHTLRPTGEWR"
     gene            complement(6570..>6854)
                     /locus_tag="VIN32_03835"
     CDS             complement(6570..>6854)
                     /locus_tag="VIN32_03835"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921744.1"
                     /translation="LLFGAWASLPTGPASLALMSGRPLMAAACWRVAPERFNARAWLI
                     EAERTGDRRADVAALTEAMARRMEEAIAVLPEQWFAAFQPIWDEEGSGGG"
BASE COUNT         1045 a         2251 c         2466 g         1092 t
ORIGIN      
        1 cgcacgcacc gcgtcctctc ggacgacggc gttccggccc tgctcgtcga gcacgtcacg
       61 aagcagttcg tggtcggaag ccggcgaaag cgcaagacgg tgatggccgt gaacgacgtc
      121 acgatccgga tccgccgggg cgagatctac ggcgtgctgg gcgccaacgg tagcggcaag
      181 tcgaccctga tccggctggt gagcacgctg ctcacgctcg acacgggacg ggtcgaggtg
      241 tttgggcacg acatcgagcg cgacgagatg gcggtcaagc gtgtcatcaa ccgggtgagc
      301 gtggacgctg ccttcttcaa gaagctgagc ccgttcgaga acctggccta cgccgcgagg
      361 ctgtacggca tggagccaaa ggccgcgcga gtggaggcga tctcgatcct ggcgcgcctg
      421 gggatcggcg agaagcggct cggccggcca ctcgagcaga tgagccgggg gatgcagcag
      481 aaggtcgcga tcgcgcgggc gctgctgacc agcccgaccc tgctgctcct cgacgagccg
      541 acgaccggcc tggatcccag atccaagctg gacgtgcaga ccttcatcga ggagctgcgg
      601 gacacgcatg acgccacgat cgtgctgacg acgcacgacc tggcggaggc ggatcggctc
      661 tgcgaccgga tcgggatcat caatgacggc cggatcgtgg tcgaggacac ccccgaggcg
      721 ctgaaggcca aggtggccga ggagcgcggg ctggagccga ccatggagag cgtcttcatg
      781 acctacaccg gacgctcgct tgacgacgac ctgggcgaca acgaagagaa ggacgacgag
      841 gactaaccga tggcactcat gccccgcgag ctgaaggcct cgttcgcctt tgtcgagcgg
      901 aacttcaacc tgacgaagcg ctactggggc tgggaggtcg ccttcctggt ctatgccgtg
      961 gcgggcgccc tggccgtgtc gctcatcggt gcggggcagg gcgacacgcg gctgctgatg
     1021 acgctggtga tcggcgccgt cttctggaac tacctgtcgg tcgtcttcag cttcatcgcc
     1081 gagaccgtca gctgggagcg ctgggaggga acgctggagt acacgttcat ggctcccgtg
     1141 cggcgctatg cccagatgct gggctcgacc gtgtacgcgg tggcgttcgg cttcgtgcac
     1201 acggtggtgg tgctggtggt gctcgccctg ttcttcgagc tcgacctgtc ccgcgccaac
     1261 ttcgcgaccg ccgcggtctt catggtgctt ggctcggtct ccttcatcgg gatcgggatc
     1321 atgacggcca tcctgccgct gatgtacgtc gagcgcgggg cgcagatgac cttcgtgctg
     1381 cagtcggtgc tgctgctgat cagcggcgtc tattacagcg tggacgtgct ccccggctgg
     1441 atgcaggtcc tctcgaactt cagcccggcg acctacgtgc tgcacggcgt ccgtgctggg
     1501 ctgatcgacg gacaggggat cacctcgctg ctcggcgatg tctggccgct gctgctgatg
     1561 gggatcgtct tcatcccgct gggcctctgg gcgtttggac gcgccgagcg ctacgccaag
     1621 cgcaccggca agctgaagcg agtggggtag ccatgcctac cgaaatccca ggtctctcac
     1681 tccgcgtcca tccgggcggc accgaaccgg ccgcgctcat ggcgccgatc gtcagtgcgg
     1741 tcgacgcggc gatcgggatc gacgagctca ccaccgcggc tgacctggag aactggctca
     1801 gtcatgcgac cgagaccttc gatccgagcc aggacgtggt gctggccgag gtggacggtg
     1861 cgctggtcgc gtacgcctcg gtcggctggg tggacacgag cgacggcctc cgtgagttcc
     1921 gcgtcggcgg ctacgtgcac cccgattggc aggggaaggg gatcgggcgc cggctccttg
     1981 cctggcagga ggagcgagcc cgcacgcacc ccgcgaccgc gaccgagcgg ccgctcgtga
     2041 tcgggacctg gtcggccgac gagaacgtcc ggaaggtgcg gcttttcacc cacgccgggt
     2101 acgagcaggt ccgcttcttc ttcgagatgc ggcgtccgac cctcgatgag atccagctgc
     2161 cgccgatgcc ggaggggctc gagatccgac cgatcgccgc caatcgcgcc agccagaagc
     2221 agctgtggga cgccgataga gaggccttcg aggaccactg gggcggcttc gacgcttccg
     2281 acacggcgtt cgaggcatgg ctcacccgcc ccaatcacaa cccggccctg tgggtcgcgg
     2341 cctgggacgg cgacgagatc gcaggcgcgg tgacgaacac gatcttcgcc gacgagaatg
     2401 cggctttcgg gatcgcccgg ggctggatgg agactgtctt cgtgcgtcgc gcgtggcgcc
     2461 gtcgcgggct cggtgctgcc ctggtggccc gtgccctcgt ccgtctgcgg gaggccggca
     2521 tgaccgaggc ggcgctgggg gtagacagcg acaacccatc gggcgcgctg gcgctgtacg
     2581 agcgtgcggg gctcgagatc catcggcgat cggctgccta tcgcaagccg atggaggcca
     2641 ggtgatgcgc tcgcagctgg acatccggat gcggccacac cgggattcgg acctccccga
     2701 gttcgtgcgg gttgcgaacg ccgccaacgc cgccgacggg attgacgagc gcaccagcca
     2761 ggcgggactt gccagctggc tgctgaccgc acgcgccaac tacgaccctg cgcacgacct
     2821 cgcagtcgcg acggttggcg aggagattgc cggctacggc tgggtcgact gggtcgagac
     2881 caccgatggc aagcgcgagt accggacgcg aggacacgtt gacccgcgct ggcgtcggca
     2941 gggggtcggg tccgcgatcc tggcccacaa cgaggcgcgg ctgctgaaga tcgcggccga
     3001 gcattccgac gacctgccgc gcgtctatgg ggcctttgcc cccgatctgc gggtgggtgc
     3061 cgtggcactg ctgtccgggg ctggctatct cccggtccgc tatttcttcg acatggtgcg
     3121 cgcgaccctc gacgagatcg aggtgccgcc actccccgag gggatggaga tgcgtcccgt
     3181 caccgacgtg gaggggtatc ggcgcctgtt tgctgcggac tcggaggcgt tcatggacca
     3241 ctggggcggc ttcgaatact cggaggccac gttccagcaa tggctgggag agcccacctt
     3301 cgatccgtcc ctgttcgtca tcgcctggga cggcgacgag atcgcggggg cggtcctcaa
     3361 cagcatcgac gccgaggaga acgaggctct cgatcgccgt cgaggcctgc tcgagggcgt
     3421 cttcgtgcgc cgtccgtggc ggcgccgtgg cctcgccgcc gccctcgtgg gtcgcagcct
     3481 ggagctgctg cgcgagcgcg gcatgaccag cgcctggctg ggcgtggacg cggacaaccc
     3541 gacaggtgcg ctcggcgtgt acgagcgcgc cgggttcgag atccatcggc gcgccagcgc
     3601 ctatcacaag ccgatggaga tcgcgcgatg acgatcaccc tgccggaagc accggcaatc
     3661 cccgatctcc agttccgcgc catgcgacgc ccggcggacg acgccgagat tgcccggctg
     3721 gtcaatgccg gcatggcggc gaccaacgtg ccgcatcggc tctcgcaggc gcaggtcacg
     3781 aactggctgg accatccctc gaacctggac ctggccgccg acctgctctt cgccgaggtg
     3841 aacgggtcgg tgatggccta caccgagggc ggctgggagc aggacaacga cggcggccgc
     3901 aactattcgg tctggggcca ggtgcacccc gactggcagc gtcgcggcct ggggacggcc
     3961 ctgctgcact ggatcgagga gcggcagagc cgggtcgcgg ccgggcatcc tgacctcgag
     4021 aagcgcctgc agagctgggc cagcgagtcc gagaccggcc gccttgccct gctcgagcgc
     4081 aacgggtacc ggatcgtgcg ctacgacttc gagatggagc gcccaaacct cgacgacatc
     4141 cagccgctgc ccctcccggc ggggattgag ctgcggcccg cgcgggagga ggacctgcgc
     4201 cgccactggg agacggaggt cgaggtcttt cgcgaccact ggggcgcgat cgacgacagc
     4261 gaggcgagct tcgagcagat gcgatcagat ccgcgtcgcg acatcggcct ctggatagtc
     4321 gcctggcggg gcgacgagat cgtgggccag gtgctgaacc ggatcgacag ggaagtgaat
     4381 gcggagctcg ggatgaagcg tggctgggtc agctcggtag gcgtgcgcgg cccgtggcga
     4441 cgccagggtg tcgcgcgggc gctggtggcc gagagcctcc gcgtcctgcg tgacgcgggg
     4501 atgaccagcg ccgggctggg ggtcgacgct gagaacgcta acggggcgct cggcgtctac
     4561 gaggcgaccg gcttccgcgt ggtgcggacg gaaagggtct accgcaagcc gctctagcgg
     4621 ccgctcgtcg ttgcaacccc ttcgcggatc tcgcgataga agtcgacgat ctgctcggtg
     4681 acgcggtccc acgagtactc gggtgccttc gctcgcccct cctcgcccat gcggtgccgc
     4741 agctcctcgt cgctggacag ccggtagagc gcggccgcca gcgcccgatg gttcttcggc
     4801 tccaccagca gcccctgcac gccgcgctgg accacgttct tgtagccgtg gatgtccgat
     4861 gcgatgatcg gcacgcctgc ggccatcgcc tccagcagca ctaccccgaa gctctcctgg
     4921 ccggtggccg gcgagcagaa gatgtcggcg ctggcgaagt agcgcgcctt ggcgtcatcg
     4981 ctcacgcggc ccaggaactc gacgtcccgg atctggcgca gccccacgaa tcggcggtat
     5041 tcgcgctgct tgggccccga gccgatgatc agcagccgcg catcgacgtg ccgcttgcgc
     5101 aggcggtgat aggcgcgcag caggtggatc agccccttgc gctcctcgaa gcggccgacg
     5161 aacaggatgt tgatcgtccc gtcgcgaagc tcctcgaacg gctcggcatc ggcgaatcgg
     5221 tcgaggtcga ccccgttggg gatgatccgg tagtcaccgg ggaagtagcg gctgatgaag
     5281 tggcgcgccg cgccgctgac cgcgatccgg ccgtggagct tggctgccag ctggccggcg
     5341 aaccacttgc cgacccagta ggagggcgag aagccgccga aggcgtggaa ggtggcgatg
     5401 ttgaccgtgt cggactggtc cagcaccgtc ggcgacagga aggggacgaa cggctcgtgg
     5461 aagtgcagga cgtcgaagtg atgctcggcc aggaggtcgc gcgcctgctg cttgaagcgc
     5521 cagcccaagg tgacgcgccc catgctgccg ttggcgggga tggcgtagcc ggtccccagg
     5581 cggatcacgc gcccctcgtc ctcgcgttcc ttgccgtact tgctggtgat gatccaggcg
     5641 tcgtggccca tccgcctcat cgcctcgtag gcgtgtcgaa cgtgttcgtt gacgccgccg
     5701 gggtgcggat acccataggg gctgacgatg ccgatcttca tcggtgccgg attggcgacc
     5761 gatggggcgg ccgctgtcgg cgcacctact gcgatgggcg gcgccggcgg agcgatcggc
     5821 tcactcatcg ccattctcct gtaggacgaa gcgtatgccg gacgtgccgg gccttggcga
     5881 ccagctggcg cccgtagacg ctgacattgt gccagtgggt ccagtactcg ccctgtgcct
     5941 cggtggtgcg atccgcgatc gccctgcggt agtcctcggc gcttgcccca gcgaaccagg
     6001 tccagccagt cccgacgtgc tcgaggacat gcgcgtccga gttgccgacc gcggcgagct
     6061 ccatcagctg gttgagggcc cgtcggtgcg gtcgccgcgc tcgcccggcg acactgggat
     6121 tcagcatctc gatggcgtcg agccgatgtc cgaggtccgg gtcgtggtgc aggcggacga
     6181 ggctgcggcg gccgaggctg ggagtgagcg gcgccatcgg gtgcggcgcg atcgcgatcc
     6241 ctccctgggc gtggatccgc tcgatcgtct cctccagggg acgaagggga gggatccgct
     6301 gggtcaggaa gagcgccagc aggtgcccgc gtcgggtcgt gatctcctcg cccacgacca
     6361 ggccgaagtg attgtcgccc gccgcatgca gctcctgcgc tcggagggcg ccgtcgatcc
     6421 gctcgtgatc cgtgatggcg atcaggtcga gctctgtgga ccgctcgacg tggtcgagca
     6481 gcgcctggac ctccatgacg ccgtccgagt acagggtgtg caggtgcatg tccgcccggc
     6541 cccgctcgcc gccgcccctc ggccttgcgt cagccaccgc cggaaccttc ctcgtcccag
     6601 atcggctgga aggctgcgaa ccactgctcg ggcaagacgg cgatggcctc ctccatgcgt
     6661 cgggccatcg cctcggtcag ggcggcgacg tctgcgcggc ggtcgccggt ccgctccgct
     6721 tcgatgagcc acgcgcgagc gttgaaccgc tccggcgcca cgcgccagca ggccgcagcc
     6781 atcagcggac ggcccgacat gagcgccagg ctggcgggac cggtgggcag gctggcccat
     6841 gcaccgaaga ggag
//
LOCUS       DATCQR010000115         4081 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_161849, whole genome shotgun sequence.
ACCESSION   DATCQR010000115 DATCQR010000000
VERSION     DATCQR010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4081
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161849"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..618)
                     /locus_tag="VIN32_03840"
     CDS             complement(<1..618)
                     /locus_tag="VIN32_03840"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEY8921745.1"
                     /translation="MCYDADARPPLPPISGAALDAGELTLTSVDGTRVAAYAARAGAP
                     SGAGIVILPDVRGLHPFFEELALRFAEAGVDAIAIDYFSRTAGTGRRDADFDYASHVP
                     LTRHETLNADVAAAAAHLRSPDGASVERLYTVGFCFGGRLSYLQAASGIDAAGVIGFY
                     GPPVGSRAGLPAPADEAPRFTCPVLAFWGGADQGIGPGDVQTFDQA"
     gene            complement(624..1781)
                     /locus_tag="VIN32_03845"
     CDS             complement(624..1781)
                     /locus_tag="VIN32_03845"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEY8921746.1"
                     /translation="MADEPQQRLAEGQRLLDAGDLDAAVQILAPLTGHPDAELGSAAW
                     LAIGDARYRLDDEPGALAAWQQAADRGGSGAWLGWRKVAEQEVRDGHLEDAVAAYREA
                     DRRAPSAERGAIANRIAWLLKETGHDFASRRQFNRARGAYGSYQPWVTWAIIAINAAV
                     FVVDAALAGGSGFSLTGGSGPLSQAGAVYGPAVAAGEWWRLITGAFLHLGILHIAFNM
                     YALWLFGPIIEQMYGHVEFAVIYLLCALGGNVLTILVAPNVPALGASGAIFGLFGLAF
                     IVSRRRQLLLGPQARAMLSRVGTLLVLNLIITFAIPYISWTGHVGGLVVGGVIGLLLA
                     PGNVPTMGGVWRAPDGSPMARRISPSLRASAYLLVAAVLVLGTYVAIQQLG"
     gene            complement(1910..2095)
                     /locus_tag="VIN32_03850"
     CDS             complement(1910..2095)
                     /locus_tag="VIN32_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY8921747.1"
                     /translation="MISTEVGKLLTATEVADLLHLHVNTVKRLGDRGEIPFYRVCKRG
                     DRRFRYDDVLDFLRRAK"
     gene            2235..2621
                     /locus_tag="VIN32_03855"
     CDS             2235..2621
                     /locus_tag="VIN32_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013568407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HEY8921748.1"
                     /translation="MPVYERHVFVCTRGEWCPSVDGDGLGVHAALKSAVRSTDLAGRV
                     RVNHSGCFSQCGNGPMVVVYPDGVWYAAVTPADAAEIVESHLLAGRPVERLRYDPPEF
                     GSHQLEREPDGRPIGRTAPWPARDEG"
     gene            2629..3549
                     /locus_tag="VIN32_03860"
     CDS             2629..3549
                     /locus_tag="VIN32_03860"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY8921749.1"
                     /translation="MTERLPAEVAQLLQQRAAARAARDWVAADALRDQIRALGWDAID
                     TSDGSTARPAAPAPASDLPSLLDQPASLDASIVVTVDDHPDDLLRLLRGLALRPPRVA
                     WELLVVANAPAQDLEPLLSGAWPGDGSLPAPTVLPLPARLGWADAVNLGLRRSRGGVA
                     ILLDTSLEPVGEIIAPLLAAFDDPAVGIAGGWGVTSGDGRDFVDAPPGLVDAIEAYCL
                     AVRREALRSVGGFDPHFRFYRHADLDFSFAVRDAGWKALRTDPLPFVRHLHRGWEAHP
                     PDERDRLSKRNFYRFLKRWRDRPDLLSGGA"
     gene            complement(3546..3872)
                     /locus_tag="VIN32_03865"
     CDS             complement(3546..3872)
                     /locus_tag="VIN32_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011605941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY8921750.1"
                     /translation="MDRSLPAPLVDLPWDREDPPDAASAAKRLRDAGVEPHAWSNGPG
                     ERYGVHSHDYIKLLVCAAGSITFLVGADAVAVELQPGDGFVLPPGTPHAAIVGPRGCT
                     CLEGYR"
     gene            complement(3881..>4081)
                     /locus_tag="VIN32_03870"
     CDS             complement(3881..>4081)
                     /locus_tag="VIN32_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014676843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8921751.1"
                     /translation="SFDEIIVTLFTAGAGTQTIPIWIFASIQRPTELPVVNVVALVLL
                     LLSVIPVYLAQRIAGETAGITR"
BASE COUNT          601 a         1449 c         1439 g          592 t
ORIGIN      
        1 cgcctggtcg aaggtctgca cgtccccagg accgatcccc tggtctgcgc cgccccagaa
       61 cgccagcacc gggcaggtga accgcggcgc ctcgtcggcc ggagccggca gcccggcgcg
      121 ggagcccacc ggtggtccgt agaagccgat cactcccgcc gcgtcgatcc ccgaggccgc
      181 ctgcagatag ctcaggcgcc cgccgaagca gaagccgacg gtgtacagcc gctcgaccga
      241 tgcgccgtcc ggcgagcgca ggtgggcggc tgccgccgca acgtccgcgt tgagcgtctc
      301 gtggcgagtc agtggcacgt ggctggcata gtcgaagtcc gcgtcgcgcc ggcccgtccc
      361 cgccgtccgg ctgaagtagt cgatcgcaat cgcgtcgacg cccgcctcgg cgaagcgcag
      421 ggccagctcc tcgaagaacg ggtgcaggcc gcgcacgtcc ggcaggatca cgatccccgc
      481 tccgctcggc gctccggcgc gggcggcgta ggccgcaacg cgggtcccgt cgacggaggt
      541 gagggtcagc tcgccggcat cgagcgccgc gccgctgatc ggggggagcg gcggtcgggc
      601 atcggcgtcg tagcacatga tcgtcagccg agctgctgga tcgcgacgta ggtgcccagc
      661 acgagcaccg cggcgaccag caggtaggcg ctggcacgca acgacggcga gatgcgccgg
      721 gccatgggcg atccgtcggg ggcgcgccac accccgccca tggtcggcac gttgccaggg
      781 gccagcagca ggccgatcac gccgcccacc accaatccgc cgacgtggcc ggtccagctg
      841 atgtacggaa tggcgaaggt gatgatcaag ttcaggacga gcagcgtgcc gacgcgagag
      901 agcatggccc gcgcctgcgg tcccagcagc agttggcgcc tgcgcgagac gatgaacgcc
      961 aggccgaaga gcccgaagat ggcccccgac gcgccgagcg ccggaacgtt gggcgcaacc
     1021 aggatggtca agacgttccc gcccagggcg cacagcaggt agatgacggc gaactccacg
     1081 tggccgtaca tctgctcgat gatcggcccg aacagccaga gcgcgtacat gttgaaggcg
     1141 atgtgcagga tccccaggtg gaggaacgcc ccggtgatca ggcgccacca ctcgccggca
     1201 gcgaccgcag gcccgtagac cgcgccggcc tgcgagagcg ggcccgaccc gccggtcagg
     1261 gagaagccgg atccccccgc gagcgccgcg tcgaccacga agacggcggc gttgatggcg
     1321 atgatcgccc aggtgaccca gggctggtag ctgccgtagg cgccgcgcgc tcggttgaac
     1381 tggcgccgcg aggcgaagtc atgacccgtc tccttgagga gccatgcgat gcggttggcg
     1441 atcgctccgc gctccgcaga tggggctcgt cggtcagcct cccggtaggc ggcgaccgcg
     1501 tcctccaggt gaccgtcgcg gacctcctgc tcggcgacct tgcgccatcc gagccaggcg
     1561 ccggaaccgc cgcggtcggc tgcctgttgc caggcggcga gggctccggg ctcatcgtcg
     1621 aggcggtacc tggcgtcgcc gatcgcgagc caggcagcgc tgcccagctc ggcatccgga
     1681 tgcccggtca ggggagcgag gatctgcacc gccgcgtcga ggtcgcctgc gtcgaggagg
     1741 cgttgcccct ctgcgaggcg ctgctgcggt tcatcggcca tcggtcaacg cattatcgtc
     1801 ggaaacgaaa cgaccgccga gagattggca cctcggcggt cgtgacgcgc ccccttggcg
     1861 ggaacgggcc gtcccggcgt gagccgggac cggcatctat tggcgcgagc tacttcgcgc
     1921 ggcggaggaa gtccagcacg tcgtcgtagc ggaaccgtcg gtcgccacgc ttgcagaccc
     1981 gatagaacgg gatctcgccg cgatcgccga gccgcttgac ggtgttgacg tgcaggtgca
     2041 gcagatcggc gacctcggtc gccgtgagca gcttccctac ctcggtactg atcatctgaa
     2101 tcggtttccc agtgctgttg aagcacgcgt ggaccgttga ggtgcggagc gcgcggcttc
     2161 tggccggata gtaaggttgg ggtgggttgg ggagccccgg tactttcgat ccgccgacag
     2221 gggtacttcc gcgaatgccc gtctacgaac gacacgtctt cgtctgcact cgtggcgagt
     2281 ggtgcccgtc ggtcgacggc gatggcctgg gcgtccatgc ggccctcaaa tccgccgtcc
     2341 gcagcacgga cctggccggg cgtgtccgcg tcaaccactc gggctgcttc agccagtgcg
     2401 gcaatggacc gatggtcgtc gtctaccccg atggcgtctg gtacgcggcg gtcacgccgg
     2461 cagacgcggc cgagatcgtc gagagccacc tgctcgctgg ccggcccgtc gagcgcctcc
     2521 gctacgaccc gcccgagttc ggctcgcacc agctcgagcg cgaacccgac ggccggccga
     2581 tcggccgcac cgccccctgg ccggcacgcg acgaaggctg accgccccgt gacggagcgg
     2641 ctgccagccg aggtggcgca gctgctccag caacgcgccg cggcccgtgc cgctcgcgac
     2701 tgggtagccg ccgatgcgct ccgcgatcag atccgcgccc tgggctggga tgccatcgac
     2761 acgtcagacg gttccaccgc ccgcccggcc gctccagcgc ccgcgtccga cctgccgagc
     2821 ctgctcgacc agccggccag cctggacgct tccatcgtgg tcacggtgga cgaccatccg
     2881 gacgatctgc tgcggctgct gcgcggcctg gcgctgcgtc ctccaagggt cgcctgggag
     2941 ctgctcgtgg tcgctaacgc cccggcacag gacctcgagc cgctgctctc cggggcgtgg
     3001 ccgggcgatg gatcgctgcc agcaccgacc gtcctgcctc tgcccgcgcg cctcggctgg
     3061 gccgatgccg tcaacctggg gctccgacgc agccgagggg gtgtcgccat cctgctcgac
     3121 acctcattgg aaccggtcgg cgagatcatc gctccgctcc tcgccgcgtt cgatgatccg
     3181 gcagtcggga tcgccggagg ctggggcgtg acgagtggcg acgggcgtga ctttgtcgat
     3241 gccccgccgg gcctcgtcga cgccatcgag gcgtactgcc tcgccgtccg acgcgaggcg
     3301 ctccgatcag tgggcggctt cgacccgcac ttccgctttt accggcatgc cgatctcgac
     3361 ttctccttcg ctgtccgcga cgcgggctgg aaggcgctcc gcacggatcc gttgccgttc
     3421 gtgcgccacc tgcaccgcgg ctgggaggct cacccgcccg acgagcgcga tcggctcagc
     3481 aagcgcaact tctaccggtt cctcaagcgt tggcgagacc gcccggacct cctgtcgggt
     3541 ggcgcctagc ggtatccctc caggcaggtg cagcccctcg gcccgacgat cgcggcgtgc
     3601 ggcgtgcccg gggggagcac gaacccgtcg cctggctgga gctccactgc taccgcatcg
     3661 gctccgacca ggaaggtgat cgagccggct gcgcagacca gcagcttgat gtagtcatgg
     3721 ctgtgcacgc cgtatcgctc tccggggccg ttcgaccatg catggggctc caccccggcg
     3781 tcgcgcagcc gcttcgcggc actggcggcg tcgggcgggt cctcgcgatc ccatggcagg
     3841 tcgaccagcg gcgctggcag ggatcggtcc atgcgtgcgg tcagcgcgtg atacccgccg
     3901 tctcgccggc gatgcgctgg gccaggtaga ccgggatcac gctcagcagg agcaggacca
     3961 gcgccacgac gttgacgacc gggagctccg tcggtcgctg gatcgaggcg aagatccaga
     4021 tcgggatcgt ctgcgtgccc gcccccgcgg tgaagagcgt cacgatgatc tcgtcgaacg
     4081 a
//
LOCUS       DATCQR010000116         1578 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_84407, whole genome shotgun sequence.
ACCESSION   DATCQR010000116 DATCQR010000000
VERSION     DATCQR010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84407"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /gene="cysM"
                     /locus_tag="VIN32_03875"
     CDS             <1..300
                     /gene="cysM"
                     /locus_tag="VIN32_03875"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016887241.1"
                     /note="catalyzes the formation of cysteine from
                     3-O-acetyl-L-serine and hydrogen sulfide; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HEY8921752.1"
                     /translation="GFIPELYDATLIDSRFSVGPRDAVRRVRELLEGEGIFAGISTGA
                     ILHTALAQAAKAVAAGGRADIVFVVADGGWKYLSTGAYEGTVDEAEDRLEGQLWA"
     gene            405..1367
                     /locus_tag="VIN32_03880"
     CDS             405..1367
                     /locus_tag="VIN32_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_100160675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
                     /protein_id="HEY8921753.1"
                     /translation="MAHANARLNVHGRRLLIERVLDDGRPVAHVAKELGVSRQCAHRW
                     INRFRVEGETGLLDRSSRPHRCPRRTSAEVEQLVLQARRRARRGQDWISAEVGVPART
                     VAAILSRWQVPRLRECDPLTGEVIRASRTTAVRYERPAAGDLVHIDVKKLGRIPDGGG
                     WLSRGQTRENHVSGHTKVGYDYIHAAVDDHSRWAYAESLADEKGTTCAAFLLRAAAAF
                     ASRGITVREVITDNAKNYVRSRDFQAALETIGARHLTIRPHCPWQNGKVERFNRTVQA
                     EWAYRQIFASNTDRTDALAPWLEHYNTRRRHSAIGGQPPITRLS"
     gene            complement(1387..>1578)
                     /locus_tag="VIN32_03885"
     CDS             complement(1387..>1578)
                     /locus_tag="VIN32_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase J family beta-CASP ribonuclease"
                     /protein_id="HEY8921754.1"
                     /translation="RQAEDDSIFDAIKQPIIDALDAQVAEGVLDNYQLQQTIRRVIGR
                     WVSNTHRRRPMIIPVVVEA"
BASE COUNT          265 a          541 c          519 g          253 t
ORIGIN      
        1 ggcttcatcc ccgagctgta cgacgcgacg ctgatcgact cgcggttctc ggtcggtccc
       61 cgcgacgccg tacgacgggt gcgggagctg ctcgagggcg aggggatctt cgccggcatc
      121 tccaccggcg cgatcctgca caccgccctc gcccaggcgg cgaaggccgt cgcggctgga
      181 gggcgggccg acatcgtctt cgtggtggcc gacggcggct ggaagtacct ctcgacaggc
      241 gcctacgaag gcacggtcga cgaggcggag gaccgcctcg agggccagct ctgggcctag
      301 gtgtactcgg cagggacgtt ggtgacgcgt tggtgcttgt gagcggggag ggcctcctgg
      361 cttagtggag atgtctgacg tctctaccag cacaggaggt cctcgtggcc cacgctaatg
      421 cccgtttgaa cgtccatggc cgtcgcctac tgatcgagcg agtgctcgac gatggtcgac
      481 cggtagcgca tgttgcgaag gagctcggtg tttctcgtca gtgcgcacac cggtggatca
      541 accggttccg agtcgagggc gagaccggcc tgctcgaccg ctcgtctcgg ccgcaccggt
      601 gcccgcgacg cacgtcggcc gaggtcgagc agctggtcct gcaagcacgg cggcgggcac
      661 ggcgcggcca ggactggatc agcgccgagg tcggtgtccc ggctagaacc gttgcagcga
      721 tcttgagccg ctggcaggtg cctcggctgc gggagtgcga tccgttgacc ggtgaggtca
      781 tccgggcctc gcggaccacc gcggtccgct acgagcgccc agccgccggc gacctggtgc
      841 acatcgacgt caagaagctc ggccggatcc ccgatggcgg tggctggcta tcgcgtggcc
      901 agacccgcga gaaccacgta tccggccaca ccaaagtggg ttacgactac atccacgccg
      961 cggtcgacga ccactcccgg tgggcctacg ccgaaagcct cgccgacgag aaggggacga
     1021 cctgcgcggc gttcctgctg cgggccgctg ccgcattcgc ctcccgtggc atcacggtgc
     1081 gcgaggtcat caccgacaac gccaagaact acgttcgatc ccgcgacttc caagccgcct
     1141 tggaaacgat cggagcccgc cacctcacca tccggcccca ctgtccctgg cagaacggga
     1201 aggtcgagag gttcaaccgc accgtccaag ccgaatgggc ctaccgacag atcttcgcca
     1261 gcaacacaga ccgcaccgac gcccttgcac cctggctcga gcactacaac actcgacgtc
     1321 gccactcagc aatcggaggc cagccaccga tcacccgact gtcatgaacc tgtcagccga
     1381 gtacacctag gcctccacga cgaccgggat gatcatcggc cgccgacggt gcgtgttgct
     1441 cacccagcga ccgatcaccc ggcggatggt ctgctgcagc tggtagttgt cgagcacgcc
     1501 ctcggcaacc tgtgcgtcca gcgcgtcgat gatcggctgc ttgatcgcgt cgaagatcga
     1561 gtcgtcctcg gcctgtct
//
LOCUS       DATCQR010000117         2605 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_177450, whole genome shotgun sequence.
ACCESSION   DATCQR010000117 DATCQR010000000
VERSION     DATCQR010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2605
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177450"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..557
                     /locus_tag="VIN32_03890"
     CDS             <1..557
                     /locus_tag="VIN32_03890"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY8921755.1"
                     /translation="AHDLVIAVNVSPRELREPTFVAEVRAALRETGLPPGRLKLEITE
                     TVTLDQGGPGTAALSSLHDLGVHLAVDDFGTGYSSLGYFRKLAVDSLKIDRLFIDGLG
                     REPGDTAIVAAAVAFASALGLEVTAEGVETDQQTEILRSLHCALGQGFRFCRPQDAEA
                     ITRVLRRGGSLRRGLAVVSRPSAA"
     gene            612..986
                     /locus_tag="VIN32_03895"
     CDS             612..986
                     /locus_tag="VIN32_03895"
                     /inference="COORDINATES: protein motif:HMM:NF039496.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEY8921756.1"
                     /translation="MNGQKTVDEYIAAFDDWRTDAMKRLREVVKEGAPHSAVAIKWAQ
                     PVWEWNGPMIWMKAYPKHVDIGFWRGTDMDDPKKVLTGDGERMRHIKITSVDDIPADA
                     LRELVKQAVQLNTAKGNPTLGR"
     gene            999..2483
                     /gene="gltX"
                     /locus_tag="VIN32_03900"
     CDS             999..2483
                     /gene="gltX"
                     /locus_tag="VIN32_03900"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEY8921757.1"
                     /translation="MSTPEPTAVRVRMAPSPTGPLHIGTARTSLYNFLFARHVGGTYV
                     LRVEDTDVARGTDEWEQDIIDNLHWLGISWDEGPQVAGGDDVGPHAPYRQSQRMELYA
                     READRLLASGAAYHCYCTPDELEAVRHEQERNHQAPRYNGRCLRLTDADRTAFEAEGR
                     RPALRLKVPPEHIRFDDLIRGEVEFDNALLGDFVIVRSNGMPLYHFVVVVDDEAMAIT
                     HVVRGEDHLSNTPKHIALIRALGYREPQFGHIPLILNPDRSKMSKRKSQTAITAYREQ
                     GYLPEAMVNFLAFLGWSPGTEEEIFSLAELAERFEIGEVHKAGAVFDKDRLDYLNGVY
                     IRSLADGQLAYRLRPFLPEQLDDGILMRLAPLLKERLVRLGDATELAAFLTETDEQVG
                     ALYGAELLLPKGRSGAEVGEALALAHDALAAVPEPDFAAGELEAICRTAAEAHGWKAG
                     DFFRPLRVAITGRLVSPPLFGSMELLGRERTISRIDAALAKLGG"
BASE COUNT          428 a          879 c          897 g          401 t
ORIGIN      
        1 gcgcccatga cctggtcatc gcggtgaatg tctcgcctcg cgaactccgc gagcccacct
       61 ttgtcgcgga agttcgtgcg gccctgaggg agaccggcct gccgccaggg cggctaaagc
      121 tcgagatcac cgagaccgtg acactggacc agggtggtcc gggaacggcc gcgttgtcca
      181 gcctgcatga cctcggcgtg caccttgccg tcgacgactt cggcaccggc tactcgtcgc
      241 tcggctactt ccggaagctc gcggtcgact ccctcaagat cgaccggctc ttcatcgacg
      301 gcctcgggcg cgagcccggc gatacggcca tcgtcgcggc ggctgtcgcc ttcgcctcgg
      361 cccttggcct ggaggtcacg gccgagggag tcgagacgga ccagcagacg gagattctga
      421 ggagcctcca ctgcgctctc ggccagggat ttcggttctg ccggccccag gacgccgagg
      481 cgatcaccag ggtcctgcga cggggcggct cgttgcgccg agggctggcg gtggtctcgc
      541 gtcccagcgc tgcctgattt ccggcttcgt cgcggtacat tgtgcgcata cgcacgagga
      601 ccagctgacc gatgaacggg cagaagaccg ttgacgagta catcgccgcg ttcgacgact
      661 ggcgcaccga cgccatgaag cgcctgcgcg aggtcgtgaa ggagggcgca ccgcactcgg
      721 cggtcgcgat caagtgggcg cagccggtct gggaatggaa tggaccgatg atctggatga
      781 aggcctaccc gaagcacgtc gacatcggct tctggcgagg caccgacatg gacgatccca
      841 agaaggtgct caccggcgat ggcgagcgga tgcggcacat caagatcacc tcggtggatg
      901 acatcccggc cgatgccctg cgcgagctcg tgaagcaggc ggtgcagctg aataccgcga
      961 agggcaaccc gacgctcggg cgttgagtgc ccgtgtcaat gagcactcct gagccgacag
     1021 ccgtccgcgt ccggatggcg cccagtccca ctggtccgct gcacatcggg acggcacgca
     1081 ccagcctcta caacttcctc ttcgcccgcc acgtcggtgg aacctacgtg cttcgggtcg
     1141 aggacacgga cgtggctcgc ggcaccgacg agtgggagca ggacatcatc gacaacctgc
     1201 actggctcgg aatctcgtgg gacgaggggc cgcaggtggc agggggcgac gacgtcgggc
     1261 cgcacgcgcc ctaccgccag agccagcgca tggagctgta cgcacgggag gccgatcggc
     1321 tcctggccag cggcgcggcc taccactgct attgcacgcc cgatgagctc gaggcggtgc
     1381 gccacgagca ggagcgcaac caccaggcgc cacgctacaa cggccgctgc ctgcggctga
     1441 ccgatgccga tcgcaccgcc ttcgaggccg agggacgccg gcccgccctg cgcttgaagg
     1501 tcccgccgga gcacatccgc ttcgacgacc tgattcgcgg cgaggtcgag ttcgacaatg
     1561 cgctgctggg tgacttcgtg atcgttcgca gcaacgggat gccgctctac catttcgtcg
     1621 tggtcgtgga tgacgaggcg atggccatca cccacgtcgt gcggggagag gaccacctct
     1681 cgaacacccc gaagcacatc gcgctgatcc gggcgctcgg ctaccgggag ccgcagttcg
     1741 ggcacatccc gctgatcctg aacccggatc ggtcgaagat gagcaagcgc aagtcgcaga
     1801 ccgcgatcac cgcctaccgc gagcagggct acctgcccga ggcgatggtc aacttcctcg
     1861 ccttcctggg ctggtcgccg ggcaccgagg aggagatctt ctcgctggca gagctggccg
     1921 agcgcttcga gatcggcgag gttcacaagg ccggcgcggt cttcgacaag gaccggctgg
     1981 actacctgaa tggggtctac atccgatcac tggccgatgg gcagctggcc taccgcctgc
     2041 gcccattcct gccggagcag ctcgacgacg ggatcctgat gcgactggcc cccctgctca
     2101 aggagcggct cgtgcggctg ggcgacgcca ccgagctggc ggccttcctg accgagaccg
     2161 atgagcaggt gggcgccctg tacggggctg agctgctcct gccgaagggg cggtcaggtg
     2221 ccgaggtcgg ggaggcgctg gcccttgcgc acgacgcgct cgccgccgtg cctgagcccg
     2281 atttcgccgc tggcgagctg gaggcgatct gccggaccgc cgccgaggcg cacggctgga
     2341 aggctggcga cttcttccgg ccgctccgcg tggcgatcac cggccgtctc gtctcgccgc
     2401 ccctcttcgg ttcgatggag ctgctcggac gggagcgcac gatctcacgc atcgacgcgg
     2461 cgctggccaa gctgggaggc tgaggtgacc gtttgcgcca cctgcggtac cgagaacgtc
     2521 gagggggcgc gattctgcaa ctcctgcggc agcccgatcg gcggccccgc ggcgcagacc
     2581 cgcgaaacgc gcaagacggt gactg
//
LOCUS       DATCQR010000118         1618 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_22411, whole genome shotgun sequence.
ACCESSION   DATCQR010000118 DATCQR010000000
VERSION     DATCQR010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1618
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22411"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..115)
                     /locus_tag="VIN32_03905"
     CDS             complement(<1..115)
                     /locus_tag="VIN32_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEY8921758.1"
                     /translation="MSPLSGLLLVHAHPDDEVFATGGLIARSIAEGRRVDLV"
     gene            complement(112..447)
                     /locus_tag="VIN32_03910"
     CDS             complement(112..447)
                     /locus_tag="VIN32_03910"
                     /inference="COORDINATES: protein motif:HMM:NF017922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HEY8921759.1"
                     /translation="MQQIINPIAVELDDTARLMRIRWDDGHLGEWHWTTLRRACPCAL
                     CSGEGNMPGIVTLDMVFTQEQTTMERVKWIGRYALAPIWADGHDTGLFTYTKLRELCE
                     CDEHRAAGG"
     gene            complement(447..>1618)
                     /locus_tag="VIN32_03915"
     CDS             complement(447..>1618)
                     /locus_tag="VIN32_03915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921760.1"
                     /translation="PETESAPLTTSLGDDDWDPKRDGDRRRPTTAEQAVPWLIGLLLA
                     LTGIVVVLMALIFIGPDGVSGLPSRSPSASVRASPSVVPSESSSTAPSIGPTATPEPA
                     FGALEMTYQDRATASSPIRLSRRDFSATTDPVVLAQDAKGVGKYAWAPDGRQGAVIIS
                     GRAVAIVAGQAPRALTEPVEAIAFADDSSTLYGLRITKAGANDKAEVLKITFATGATE
                     VMTTITYPHAEIFPDPALKEAQFADNGGIVRLYVTVDGYVVAWILGDPSKTYRIDPAD
                     GTYTVVDAQPVLWSPDQKMQVKVNEKSGTTTLTEEGHDGVAKASAKIIGLVSHLRWAG
                     GGNEVVFTLGRLVGGGVRQDLYVWDLVNGKAPAPLTSNGASFGAEWLGVLQSWVP"
BASE COUNT          227 a          555 c          579 g          257 t
ORIGIN      
        1 ccaccaggtc gacccggcgc ccctcggcga tcgagcgggc gatcagcccg ccggtggcga
       61 agacctcgtc gtcggggtgg gcgtggacga ggagcaggcc ggagagtggg ctcatccgcc
      121 tgccgcccga tgctcatcgc actcgcacaa ctcgcggagc ttcgtgtacg tgaagaggcc
      181 cgtgtcgtgg ccatcggccc agatgggggc cagggcgtat cggcctatcc atttgacccg
      241 ctccatggtc gtctgctcct gggtgaagac catgtcgagg gtcacgatgc ccggcatgtt
      301 cccctcgccc gagcagagcg cgcagggaca ggcgcgacgc agcgtggtcc agtgccactc
      361 gcccaggtgg ccgtcgtccc agcggatgcg catcaggcgg gcggtgtcgt ccagctcgac
      421 cgcgatcggg ttgatgatct gctgcatcag gggacccagc tctgcagcac gcccagccac
      481 tccgcgccga acgaggcgcc gttgctggtg agcggcgccg gcgccttgcc gttcaccagg
      541 tcccagacgt agaggtcctg gcgcacgccg cccccgacca ggcggccgag cgtgaagacg
      601 acctcattgc ccccgcccgc ccagcgcagg tggctgacga gcccgatgat cttggccgat
      661 gccttggcca ccccgtcatg cccctcctcg gtgagggtgg tggtccccga cttctcgttc
      721 accttcacct gcatcttctg gtcgggcgac cagaggaccg gctgcgcgtc caccacggtg
      781 tacgtcccgt cggccgggtc gatccggtag gtcttgctgg gatcgcccag gatccaggcc
      841 acgacatagc cgtcgaccgt cacgtacagc cgcacgatgc cgccgttgtc ggcgaactgg
      901 gcctccttca gggcggggtc ggggaagatc tcggcgtggg ggtaggtgat cgtggtcatg
      961 acctcggtcg ccccggttgc gaaggtgatc ttgagcacct ccgctttgtc gttcgctcct
     1021 gccttcgtga tgcgcaggcc gtagagcgtg ctggagtcat cggcgaaggc gatcgcctcc
     1081 actggctccg tgagggcgcg tggagcctgg ccggccacga tggcgaccgc ccgcccggag
     1141 atgatcactg cgccctggcg cccatccggc gcccaggcgt acttgcccac ccccttggca
     1201 tcctgcgcca gcaccaccgg gtcggtggtg gcggagaagt cgcgacgcga gaggcggatc
     1261 ggggaggagg cggtggcccg gtcctggtag gtcatctcga gcgcgccgaa ggcgggctcc
     1321 ggcgttgcgg ttggcccgat cgacggcgcg gtggacgacg actcggatgg gaccacgctc
     1381 gggctggccc ggaccgatgc cgagggcgat cggctcggca ggccggagac tccatcgggg
     1441 ccgatgaaga tgagcgccat cagcacgacc acgatcccgg tcagcgccag cagcaggccg
     1501 atcagccagg gcaccgcctg ctcggcggtc gtcgggcggc ggcgatcccc atcgcgcttg
     1561 gggtcccagt cgtcgtcgcc gagcgaggtc gtcagcggcg cggactcggt ctctgggg
//
LOCUS       DATCQR010000119         7421 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_146450, whole genome shotgun sequence.
ACCESSION   DATCQR010000119 DATCQR010000000
VERSION     DATCQR010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 7421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7421
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146450"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="VIN32_03920"
     CDS             <1..217
                     /locus_tag="VIN32_03920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921761.1"
                     /translation="EGSHRRRSVFSMEDSEREVEILAEVVEREHRRLELIRDVGGAPS
                     LEASAEPIRPVPSTVTFAAISASSASD"
     gene            338..1462
                     /locus_tag="VIN32_03925"
     CDS             338..1462
                     /locus_tag="VIN32_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002627679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY8921762.1"
                     /translation="MANQTESAGSKLGVGIIGVNPAWGWGATAHIPALRALPNYEVRA
                     LSATSADAARAAGEAFGVAAVFSHHDELVAQPDVDIVAVTVKVPHHRELVSAALAAGK
                     AVYCEWPLGRDLDDARAMAALAAEQRVRTVVGLQARQAPAIEFVQELLRDGYVGEVLS
                     TTMVGLSVPGNVVIQPNVYMLDRNNGANVLTVAFGHSLDTLNHVLGEFADLSAVSAIR
                     RPLISAEETWEQIVKTAPDQIAVIGTLTSGAIASIHVREAVAGGIGFLWEINGTEGTL
                     RITADAALPEIFPLTVTGARGAGEPSELAVPRALTGKWPALNDLEGSPAYNVGRAYAA
                     FAADLDNGTHTVPDFVDAVRRHEVIAAIERSAASGARVRV"
     gene            1506..2171
                     /locus_tag="VIN32_03930"
     CDS             1506..2171
                     /locus_tag="VIN32_03930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEY8921763.1"
                     /translation="MKGPTRRVVTATLILALGLAACAASPGGSPPIDAAAAARLALAQ
                     QDRFAGIGPHDEKLIGQAAWYEVTDVEDGWQVLIRIGWGDCEAGCINEHRWTYAVART
                     GKVELTHEEGDALPDATGVQGTVTAGPTCPVETEPPDPACAARPVAGAVLVFTNAGGA
                     EVARGTSAADGTFTVELAPGAYRLTPQSVKGLMGTPAVMDVVVEAGLPTELQVSYDTG
                     IRG"
     gene            complement(2168..2374)
                     /locus_tag="VIN32_03935"
     CDS             complement(2168..2374)
                     /locus_tag="VIN32_03935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921764.1"
                     /translation="MIRYLALPILIAAVGAVYWLASKGEPAGSALLLIFAAAMAVFLW
                     VLLPTADNVGPTAPVDPDFEPKHR"
     gene            complement(2452..3024)
                     /locus_tag="VIN32_03940"
     CDS             complement(2452..3024)
                     /locus_tag="VIN32_03940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921765.1"
                     /translation="MIDPIARLRTEDTIVYELLQGSQGSQSSGDGTVVGTATFRDGAS
                     RVKAPESISLAVEELLARPFVDRVQADERPRGYRRSRVGQVDLLVPGMPEHFVARMRG
                     LWLAYPDGSLITARPAVGTPPVPGPRAALPDLGEGEAPVTDPAVRRATLADSSELLGA
                     GPLVHASEPATGVRITEGRALARTDCGWLV"
     gene            3131..3487
                     /locus_tag="VIN32_03945"
     CDS             3131..3487
                     /locus_tag="VIN32_03945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921766.1"
                     /translation="MSATEERSYRTMVEEQADQQIDTWAADLFIDFAKRMGVGTAIAA
                     FCAVADLDARGFQRAFLVGGGPDHVVGIDTAGALAAPIFELPRAIAGLRRTDPEARRK
                     LVAFLVGQRDVMSYTP"
     gene            complement(3492..4466)
                     /locus_tag="VIN32_03950"
     CDS             complement(3492..4466)
                     /locus_tag="VIN32_03950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEY8921767.1"
                     /translation="MASKRPAFSENIHRLFDEAQATDLAPGIVSLIESRATHAQIREV
                     PTNRILPNPAQPRLSYEEESLVELADSIREHGVLQPILVRPVGSQFELIAGERRWRAS
                     RMAQRESIPAIVVEFDDETALEVSIIENLQREDVSPLEEAAMFRKMTDLGYSVRQLAQ
                     KIGKDKGYVENRIRLAEAPPEIRELVSLRKDTLSHAYELMKVRDERTRRRLAKKVAAG
                     ELTLSRLRALIGGGSAPEVEIDAKPKRRARTIAGQVAASKDADDALVNNRVRLAGAID
                     ELVDLLSQRELIDEIPDTNRDNLARYLTVTKLKLENAIAIIRATREPA"
     gene            complement(4459..5226)
                     /locus_tag="VIN32_03955"
     CDS             complement(4459..5226)
                     /locus_tag="VIN32_03955"
                     /inference="COORDINATES: protein motif:HMM:NF022077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY8921768.1"
                     /translation="MTKVISIANQKGGVGKTTASINLGGALAELGYRVLCIDMDPQAN
                     LTVGLGISLSDVRHSMADVLSENRVPLDEIVRQTEMPGLSVAPSTLELASTEVELFTA
                     IGREMVLRDALDGWAERQYDVVIIDSPPTLGLLTINALVASSRVIIPVQTQFYAIKGL
                     TALIKVINTIKIKLNHDLEILGLLATFYDGRTVLAREMLQNLRELGDHRVFSTMIKQT
                     VKLGEAPLTGKPVTEYATHSAAARAFRDLAQEVIELG"
     gene            5396..5471
                     /locus_tag="VIN32_03960"
     tRNA            5396..5471
                     /locus_tag="VIN32_03960"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5429..5431,aa:Ile,seq:cat)
     gene            5490..7040
                     /locus_tag="VIN32_03965"
     CDS             5490..7040
                     /locus_tag="VIN32_03965"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014393925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HEY8921769.1"
                     /translation="MSTADKLANLRRLREEARLGGGEQRIAQQHDRNKLTARERIELL
                     LDEGSFVELDAFVTHRATDFGLDDERYLGDGVVSGHGRVDGRLVFVYAQDFTVFGGSL
                     SEAHAEKICKVMDLAMSTGAPIIGLSDSGGARIQEGVASLGGYAEIFLRNTLASGVVP
                     QISVVMGPCAGGAVYSPAITDFTIMVDGSSYMFVTGPNVVKTVTHEEIDLEGLGGARV
                     HNETSGVAHFLAEGEPQAMELTRRILSYVPQNNIDPPPVATDWRAPEVSGADLDALVP
                     DSAQRSYDMRRVIAGIVDAESFTEVHAHFARNVICGFARVEGRSVGIVAQQPEVLAGV
                     LDIDASDKAARFVRFCDCFNVPLVTLVDVPGFLPGVDQEHRGIIRHGAKLLYAYCEAT
                     VPKVTVITRKAYGGAYDVMSSKHVRGDLNFAWPTAEIAVMGVEGAVNIIFRDRLAEAA
                     DPDAERAGLVAEYEERFANPYVAAARGYVDEVILPSETRARVVDALAMLQGKRQAVPA
                     RKHGNIPL"
     gene            7051..>7421
                     /locus_tag="VIN32_03970"
     CDS             7051..>7421
                     /locus_tag="VIN32_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HEY8921770.1"
                     /translation="MRKDGPPFRRLLVANRGEIAVRIIRACRDLGIESIAAYSDADAD
                     ALHTRMADRAERIGPARAAESYLSIEAVLAAAARSGAEAIHPGYGFLSENAEFARAVE
                     DAGLVFVGPPPETLASLGDKLA"
BASE COUNT         1231 a         2395 c         2555 g         1240 t
ORIGIN      
        1 cgagggcagt catcggcgcc ggagcgtgtt cagcatggaa gactccgagc gcgaggtcga
       61 gatccttgct gaggtcgtgg agcgcgaaca tcgtcggctg gagctcatcc gcgacgtagg
      121 cggcgcgccg agtctcgagg cgagcgccga gcccatccgg cccgtgccaa gcaccgtgac
      181 gttcgcggcc atcagcgcct cctccgcgag tgattgactt gggccggagc cttggtcagt
      241 cagtttacgc tcgcgcgatc gcctcccgaa actacttgca cgtgcaacca actgagcgta
      301 aggtagttgc ggtcgcaagt atcgttgaga ggcgtcattg gccaaccaaa ctgagtccgc
      361 tggatcaaag ctgggtgtcg ggatcatcgg ggtcaatcct gcctggggtt ggggagccac
      421 tgcgcacatc cctgccctgc gggcactgcc gaactacgag gtccgggcac tcagcgcgac
      481 cagcgcggac gccgcgcgcg ccgccggcga ggcgttcgga gtcgccgcgg tcttctcgca
      541 ccacgacgag ctggtggccc agcccgatgt cgacatcgtc gccgttacgg tcaaggttcc
      601 gcaccatcga gagctcgttt ccgcggcgct ggccgcgggc aaggccgtct actgcgagtg
      661 gccgctgggc cgcgacctcg acgacgcgcg ggcgatggcc gcgcttgccg ccgagcagag
      721 ggtgcgcaca gtcgtcgggt tgcaggcccg ccaggcaccc gcaatcgagt tcgtccagga
      781 gctgctccgc gacggatacg tcggcgaggt cctgtcaacg accatggtcg ggctgtcggt
      841 cccgggcaac gtggtgatcc agcccaacgt ctacatgctg gacaggaaca acggcgccaa
      901 cgtgctcacg gtcgccttcg gccacagcct ggacactctc aaccatgtcc tgggcgagtt
      961 cgccgacctg tcggccgttt cggcgatccg ccggccgctg atctccgccg aggagacctg
     1021 ggagcagatc gtgaagacgg cccccgacca gatcgccgtg atcggcacgc tcacatcggg
     1081 tgcaatcgcc agcattcacg tccgtgaggc agtcgccggc ggaatcgggt tcctgtggga
     1141 gatcaatgga acggagggaa ccctccggat tacggctgac gccgccctcc ccgagatctt
     1201 tccgttgacc gtcacgggag cgcgtggcgc gggcgagccc agcgagctcg ctgtcccgcg
     1261 tgcgctgacg gggaagtggc ccgcgctcaa cgatctcgaa ggctcgcccg cctacaacgt
     1321 cgggcgagcc tatgcggcct tcgcggcaga cctcgacaac gggacgcata cggtgccgga
     1381 cttcgtcgac gccgtccgac gccacgaggt catcgccgca atcgagcggt ccgcggcatc
     1441 aggggcacgc gttagggtgt agcccagtga tggaaccccg ggcccgtgac tcgcgtccgg
     1501 gcggcatgaa gggcccaact cgtcgcgtgg tcacggccac gctgatcctt gccctcgggc
     1561 tcgccgcctg tgcggcgagc cctgggggct ctcctcccat cgatgcagcg gccgctgccc
     1621 ggctggcgct cgcccagcag gaccgcttcg cagggattgg cccgcacgac gagaagctga
     1681 tcggccaggc cgcctggtac gaggtcacgg atgtcgagga tgggtggcag gtgctgatca
     1741 ggatcggttg gggagactgc gaggccgggt gcatcaacga gcatcggtgg acttacgcgg
     1801 tggctcgcac tggaaaggtc gagctgaccc acgaggaggg ggatgccctg cccgatgcga
     1861 ccggcgtaca gggcacggtg acagccggac cgacctgccc ggtcgagacg gagccgccgg
     1921 atcctgcgtg cgccgcgcga ccggtggccg gagccgtcct cgtgttcacc aacgcgggcg
     1981 gcgccgaggt ggcgcgaggg acctccgcgg ctgacggaac gttcacggtc gagctcgccc
     2041 ccggtgcata ccggctcacg ccacagtccg tgaagggctt gatgggaacc cctgcggtga
     2101 tggatgtggt ggtcgaggcc gggctgccga ccgagctgca ggtgtcctat gacaccggga
     2161 ttcgcggcta gcggtgcttc ggctcgaagt ccgggtcaac cggggcggtt gggccgacgt
     2221 tgtcagccgt cggtaacagg acccagagga agaccgccat cgccgcggcg aagatgagga
     2281 ggagggcgga gccggcaggc tctcccttgc tcgccaacca gtagacggcc ccgaccgcgg
     2341 cgataaggat cggcagcgcc aggtagcgga tcacgatggg cgagatgata gctgagcggt
     2401 cgggcacggc cgggcgccac gcgacgcggt gaatctgggc attactatgc cttagaccaa
     2461 ccatccgcaa tcggtgcgcg ccagcgcgcg accctcggtg attcggactc ccgtggccgg
     2521 ctcgctggcg tgaaccagcg gacccgcgcc cagcagctcg ctgctgtcgg ccagcgtcgc
     2581 tcggcgtacc gcagggtcgg tgaccggcgc ctcaccctcg ccgaggtcgg ggagcgctgc
     2641 ccgtggaccg ggaaccggtg gcgtcccgac cgcagggcga gcggtgatca gcgagccatc
     2701 cgggtaggcg agccagaggc cgcgcatgcg cgccacgaag tgctcgggca tgccgggtac
     2761 gaggagatcc acctggccga ctcgactgcg gcggtagccg cgcggccgct catccgcctg
     2821 gacccggtcc acgaagggac gcgccaggag ctcctcgacg gccagcgaga tcgactccgg
     2881 ggctttgacc ctcgacgcac catcacggaa ggttgcggtg cccacgaccg tgccgtcgcc
     2941 gctgctctgg ctgccctggc tgccctggag cagctcatag acgatcgtgt cctcggtccg
     3001 cagtcgcgcg atggggtcga tcacggccgc aggatagccg atgctggagg ggcctcaccg
     3061 gcgcatcggc gcagtcagcg cggggcggct tgggtagaat cgcgaggcca aatacgagcg
     3121 gaggagccgg gtgagcgcga ccgaagagcg tagctatcga acgatggtgg aggagcaggc
     3181 ggaccagcag atcgacacct gggcggccga cctcttcatc gacttcgcga agcggatggg
     3241 agtggggact gccatcgcgg cgttctgcgc agtcgccgac ctggacgcca ggggattcca
     3301 gcgggccttc ctggtgggcg gtggaccgga ccacgtggtc ggcatcgaca ccgcaggcgc
     3361 actggccgcg ccgatcttcg agctgccgcg ggcgatcgcc ggcctgcgcc gaaccgatcc
     3421 cgaggctcga cgcaagctgg tggcgttcct cgtcggccag cgtgacgtga tgagctacac
     3481 gccctgaggg gctacgccgg ctctcgggtg gctcgaatga tcgcgatggc gttctcgagt
     3541 ttcagtttgg ttactgtcag gtaacgcgcc aggttgtccc ggttcgtatc cgggatctcg
     3601 tcgatcagct ctcgctggct caacaggtcg accagctcgt cgatcgcgcc ggccagccgc
     3661 acgcgattgt tgaccagggc gtcgtccgcg tccttagagg cggcgacctg cccggcgatc
     3721 gtccttgcac ggcgcttcgg cttggcgtca atctccacct ctggggcaga gccgccaccg
     3781 atgagcgccc ggagacggga gagcgtcaac tcgccggccg caaccttctt ggcgaggcgc
     3841 cgacgcgtcc gctcgtcccg caccttcatc agctcatagg catggctcag agtgtctttg
     3901 cgcaaggaca cgagctcgcg gatctcgggc ggggcctcgg ccagtcggat gcggttctcg
     3961 acatagccct tgtccttgcc gatcttctgg gctagctgac gcaccgaata cccgaggtcg
     4021 gtcatcttcc ggaacatggc cgcctcctcg agaggggaga cgtcctcgcg ctggaggttc
     4081 tcgatgatcg agacctcgag ggcggtctca tcgtcgaact cgacgacgat cgccgggatc
     4141 gattcccgct gcgccatccg tgatgcgcgc cagcgtcgct cgccggcgat cagctcgaac
     4201 tggctgccga ccgggcgcac caggatcggc tggaggaccc catgctcacg gatcgaatct
     4261 gccagttcga ccagcgactc ctcttcgtat gagagccggg gctgcgcagg gtttggcagg
     4321 atccgattcg tgggcacctc ccggatctgc gcgtgcgtgg cacgagactc gatcagcgag
     4381 acaatgcccg gcgcgaggtc ggtggcctgt gcctcgtcga agaggcggtg aatgttttca
     4441 ctgaaggccg gtcgcttgct agccaagttc gatcacctcc tgcgccaggt cacggaatgc
     4501 gcgagctgct gccgaatggg tcgcgtattc cgtgaccggc ttcccggtca gcggcgcctc
     4561 gccgagcttc acggtctgct tgatcatggt cgagaagacc cgatgatcac cgagctctcg
     4621 caggttctgc agcatctcgc gcgcgagcac ggtgcgcccg tcatagaagg tggccagcaa
     4681 tcccaggatc tcgaggtcgt ggttgagctt gatcttgatc gtgttgatca ccttgatcag
     4741 cgccgtcaaa cccttgatcg cgtagaactg ggtctggacc gggatgatca cgcgcgaact
     4801 cgcaaccagg gcgttgatgg taaggagtcc cagcgtcggc ggcgagtcga tgatgacgac
     4861 gtcgtattga cgctcggccc acccgtcgag tgcgtcgcgc agcaccatct cgcgaccgat
     4921 tgcggtgaag agctccacct cggtcgaggc cagctccagg gtggaaggtg cgaccgacag
     4981 gccgggcatc tcggtctgcc gcacgatctc atcgagcggc actcgattct cggagaggac
     5041 atcggccatg gagtgcctga cgtccgagag gctgatgccc aggccaacgg tcagattcgc
     5101 ctgcgggtcc atatcaatgc agaggacccg ataccccaac tcggcgagcg caccgccgag
     5161 attgatggag gcggtcgtct ttccgactcc gcccttctgg ttcgcgatgg agattacctt
     5221 ggtcacaaga tcaccttgca gcggctcggg cgggaaggga aaggtcgcgg atgcgacgga
     5281 ccgatgatac ctgcgagcga ccgtccgcga ccatccgagt tatccacaag ctgccggaat
     5341 gtcgatgtct ttgtgcaaag acgagcatct atactccgac ttgtccacca tcgtcgggcc
     5401 catagctcag tggttagagc atccggctca taaccggcgg gtcgcaggtt cgatccctgc
     5461 tgggcccacc accagcgcgg aggacgccaa tgtcgaccgc cgacaagctt gcgaatcttc
     5521 ggcgcttgcg cgaggaggct cgtctgggcg gcggcgagca acgcatcgca cagcagcacg
     5581 atcgcaacaa gctcaccgcg cgcgagcgga tcgagttgct gcttgacgag ggatcgttcg
     5641 ttgagctgga cgcattcgtg acgcatcggg cgacggactt cggactggac gatgagcgct
     5701 atctcggcga cggcgtcgtg agcgggcacg ggcgcgtcga tggccggctg gtcttcgtct
     5761 atgcgcagga cttcaccgtc ttcggtggat ccctttccga ggcgcacgcg gagaagatct
     5821 gcaaggtgat ggacctggcg atgagcaccg gcgcgccgat catcgggctc tcggattctg
     5881 ggggcgcaag gatccaggag ggggtcgcct cgttgggagg ctatgcggag atcttcctgc
     5941 gcaacaccct cgcctcgggt gtcgtgccgc agatctcggt ggtgatgggg ccgtgtgccg
     6001 gcggcgcggt ctactcgccg gccatcaccg acttcaccat catggtcgac ggcagcagct
     6061 acatgttcgt gaccgggccg aatgtcgtga agaccgtgac ccacgaagag atcgacctcg
     6121 aagggcttgg tggggcgcgg gttcacaacg agacgagcgg cgtagcgcat ttcctggccg
     6181 agggggagcc acaggcgatg gagctgacgc gacgaatcct cagctacgtg ccgcagaaca
     6241 acatcgaccc gccccctgtc gccacggact ggcgggcgcc ggaggtcagc ggcgccgacc
     6301 tcgatgccct ggtgcccgat tccgcgcagc gctcctacga catgcgtcgc gtcatcgccg
     6361 ggatcgtcga cgccgaatca ttcaccgagg tgcacgcgca tttcgcgcgc aacgtcatct
     6421 gtggattcgc gcgagttgag ggcaggagtg ttggcatcgt cgcgcagcag cccgaggtcc
     6481 tggctggcgt actcgacatc gacgcgtcgg acaaggcggc gcgcttcgtc cgcttctgcg
     6541 actgcttcaa tgttccgctc gtcacccttg tcgacgtgcc gggcttcctg cccggtgtcg
     6601 accaggaaca tcgcggcatc attcgtcacg gcgccaagct gctctatgcg tactgcgaag
     6661 cgacggtgcc aaaggtgacc gtcatcacgc gcaaggccta cggtggcgca tacgacgtca
     6721 tgagcagcaa gcacgttcgt ggtgacctga acttcgcctg gccgacggcg gagatcgcgg
     6781 tgatgggcgt cgagggcgct gtcaacatca tctttcggga tcgcctggca gaagcagccg
     6841 atccagatgc agaacgcgcg ggccttgttg ccgaatacga ggagcgcttt gcgaatccgt
     6901 acgtcgccgc cgccaggggc tacgtcgacg aggtgatcct tccgtccgag acacgggcgc
     6961 gggtggtgga cgcgctggcc atgcttcagg gaaagcgtca ggcggtgccc gcccggaagc
     7021 atggcaacat cccgctctga ctgcccgtct ttgcgcaaag acgggccccc atttcgacgg
     7081 ctcctcgtag ccaaccgcgg tgagatagcg gtccggataa tccgcgcctg tcgcgacctg
     7141 gggatcgaat ccatcgccgc ctacagcgac gccgacgcgg atgcgctgca cacgcgcatg
     7201 gcagatcggg cggagcgcat cggccccgcg cgggctgcgg agtcatacct ttccatcgaa
     7261 gccgtgctgg ccgccgccgc ccgttcgggg gcagaggcga tccacccggg ctacggattc
     7321 ctgtccgaga acgcggagtt tgcccgcgcc gtcgaggatg cggggctcgt cttcgttgga
     7381 ccgccgccgg agacgctggc atcgctcggc gacaagctgg c
//
LOCUS       DATCQR010000120        21127 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_115630, whole genome shotgun sequence.
ACCESSION   DATCQR010000120 DATCQR010000000
VERSION     DATCQR010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 21127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21127
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115630"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..466)
                     /locus_tag="VIN32_03975"
     CDS             complement(<1..466)
                     /locus_tag="VIN32_03975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921771.1"
                     /translation="MPPDPAAAGQPAAGILIALDALIVQRADQPVALDEAPVALRRIG
                     WIGRPIILVGRQVAGRDLPSDAREREAWVRASIGRGAYRVVDFQDPTADRRLSQDGHG
                     VERWGEVRDAQEGTWLLTDRPRQVGAARKAGLKVILIGPPDPQPRGQRPDYQA"
     gene            complement(456..1220)
                     /locus_tag="VIN32_03980"
     CDS             complement(456..1220)
                     /locus_tag="VIN32_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY8921772.1"
                     /translation="MNNQPLVLVADDEPRITKLVAMSLADEGFRVVTAGGGEEALRKA
                     EEMRPDIVLLDVVMPDLDGIEVMRQLREWRPVPVILLTAKGSTSDKAKGLDLGADDYI
                     AKPFHPDELAARVRAVLRRAAGITPAGGILHIGEVEIDLERRMMSRNGQLVQLSRTEW
                     LLLQHLAANAGKVVLHTELLTKVWGPEYRDDLQYLRVWISRVRRKLGALPGEPGPITT
                     FQGIGYVLNVDETPPLEMPRDGAEVGDARAVGGHAP"
     gene            complement(1308..1766)
                     /locus_tag="VIN32_03985"
     CDS             complement(1308..1766)
                     /locus_tag="VIN32_03985"
                     /inference="COORDINATES: protein motif:HMM:NF014389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEY8921773.1"
                     /translation="MDEERVVERQSVTTPPADTTTSVEPVQPVVEPARYPNETVSNSS
                     VVSETVRRSPSGAEVARRVVVFIFGIIQALIVIRIVLLLADANQANQLVRFIYDASGF
                     FVGPFEGILNTNAVRAGASIFDIAALVALIGWTILEVLIIALIGIARRES"
     gene            1993..5493
                     /locus_tag="VIN32_03990"
     CDS             1993..5493
                     /locus_tag="VIN32_03990"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008476061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0032549 - ribonucleoside binding [Evidence
                     IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEY8921774.1"
                     /translation="MALAESASRFELAPIRENFSHIPEVLPLPNLIQTQTESFNWFVQ
                     TGLRELLDEITPITDFTGKNLELHFRDYYFEEPKFDEDECRTRDLTYSKPLKVEVDLI
                     VKETDEVKHQTVFMGDFPWMTQQGTFIINGAERVVVSQLVRSPGVYYTGSEDPATGRI
                     LFAAKVIPNRGAWLEFETSNKDMLSVKVDRKRKIPVTTLLRAVGVEDNAEMLRTFGPA
                     DDHPEHPYVSLTLDKDPAATQDEALIEVYKKLRPGDPPTVDNARALVQSLFFNFRRYD
                     LGRVGRYKLNKALGDVAAKLKIALPAEGGETTRESRVISQNDIVAIIGKLIELNNGRG
                     TGDDIDHLGNRRIRANGELIQNQFRIGLLRMERVVKERMTITDAEQATPNSLINIRPV
                     VAAMKEFFGGSQLSQFMDQTNPLAELTNKRRLSALGPGGLSRERAGFDVRDVHPSHYG
                     RMCPIETPEGPNIGLIGSLATYGKINEHGFIETPYREVRKFVSHRDKQADVVGETLAE
                     DLLVGERGKKLAKAGAPIDEKLWAAIKGNKLASVRIVPVVTAQIKYLAADEEEKFYIA
                     QANAPLDEGNHFVEERVMVRYRDEFHVQVNEDVDYMDVSPKQIVSVATAMIPFLEHDD
                     ANRALMGSNMMRQSVPLLQPEAPVVGTGVEYRAAFDSQQVVVAEAGGTVVSVTSADIQ
                     VERDEGDVRDTYHLKKFLRSNQGTCINQRPIVDVGDRVAAGQVLADSSSTDKGEMALG
                     QNILVAFMPWEGGNYEDAIVISERLVREDLFTSIHIEKHEIEARDTKLGPEEITRDIP
                     NVGEESLRNLDEDGIIYEGAEVAPGDILVGKITPKGETELTAEERLLRAIFGEKAREV
                     KDSSLRLPHGERGTVVNVRQFSRESGDELLPGVNRLVRVSVAQKRKIAVGDKMAGRHG
                     NKGVIAMILPVEDMPLMPDGTPVDIVLNPLGVPSRMNIGQILETHLGWAMKVLGLKAA
                     TPVFDGATEAQITASLKKAAAVKGSVVSEDGKTVLRDGRTGEAFDHRVTVGYIYMLKL
                     HHLVEDKIHARSTGPYSLITQQPLGGKAQFGGQRFGEMEVWALEAYGAANILQELLTV
                     KSDDVMGRVQTYEAIVKGEEIQPPGVPESFKVLIKELQALGLSVEILNENEEEIRFIE
                     DTGSYPLPDLGGINLQGFEE"
     gene            5616..9956
                     /gene="rpoC"
                     /locus_tag="VIN32_03995"
     CDS             5616..9956
                     /gene="rpoC"
                     /locus_tag="VIN32_03995"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEY8921775.1"
                     /translation="MLEVNNFDAIRISLASPDQIKSWSSGEVTKPETINYRTLKPEKD
                     GLFDERIFGPTKDWECYCGKYKRIRYKGIICDKCGVEVTRSKVRRERMGHIKLASPVS
                     HIWYFKGTPSRLGILLDVSPRNLERILYFALYVVSRVDEHEREKALARVEEEMAERIG
                     RAGELVEAKKAELEAAIADKRTQVTGDAEADAARADGLIATRSEELAAAGQQVEAALK
                     EGGKTVAEDIVFTPTGEVVARAGEASKGALVALRKAVKAEVDALEKEVKEAKAHAAER
                     LQAAIADLSSGIDDALASEKAQVARETDDARLAARAARAQIADIKVMQTLTESEYRSL
                     SEQYGRLFDAGMGAEAIKKIIEQLDLDDLAQRLHVEMRQTSGQRRKKAIKRLRLIEAF
                     RKSGARPEWMILSTLPVIPPDLRPMVQLDGGRFATSDLNDLYRRVINRNNRLSRLLDL
                     EAPEIIIRNEKRMLQEACDALIDNGRRGRAIAGTGNHRLKSLSDMLKGKQGRFRQNLL
                     GKRVDYSGRSVIVVGPELKLHQCGLPKKMALELFKPFVMRQLVEKGFAHNIKSAKRIV
                     ERVRPEVWDVLEEVIQDHPVLLNRAPTLHRLGIQAFMPVLVEGSAIQIHPLVCFAFNA
                     DFDGDQMAVHVPLSTAAQREAKELMLSTQNLLSAADGSPVVSPTHDMVLGCYYLTIEE
                     PTEKGAGKAFASANEAVMVTQLGHVHVQAPIKVELEVWDAAANDGAGEMRTELVETTA
                     GRVIFNTVIPKELGFVNREMDRKGLKALIAQCYRELGPAATTQLVDGIKTLGFHYATM
                     AGITIGIGDIRVPTEKVKLLAGADAKVTEIDREFRRGFITEDERYNQTVDVWRGTTDA
                     VTEKMLAGLDKRGSVWMITHSGARGNVTQVHQLAGMRGLMADPSGRIIDLPIRSNLRE
                     GMSVLEYFISSHGARKGLADTALRTADSGYLTRRLVDVAQDVITREDDCGTDLGTWIT
                     REESTRIPEPFVDRLVGRMAALDLVDEKAGQQIVARNDEIDEVAIERIDALGIDRVSV
                     RSPLTCAARHGVCRMCYGRNLATGNLVDLGQAVGIIAAQSIGEPGTQLTMRTFHTGGV
                     AGEDITQGLPRVEELFEARIPKGQAIMTEIDGTIEVVRAGTDQPVVVRVTHRDTYDTP
                     IKLTPQHELLVANGDEVADGQLLARIGEGADAVEVKSPAAGRVTKKGSTLTLHSEDID
                     TREYQVPHSARLRVETGEEVLAGHPLTEGSLNPKDLLLIKGVDTVQRFLVAEVQTVYR
                     SQGVTIADKHIEIIVRQMLRKVTVDAAGDTELLPSELIDRFEFEEVNNRILAEGGEPA
                     TAQTVLLGVTKASLNTSSFLAAASFQETTRVLTEAAINGSKDHLLGLKENVIIGKLIP
                     AGTGAESRAAAAAKARELSPEEAESLREREAAMTFLTGDEEVDGDGSSATDGVEPAAE
                     EAPAAVVEEG"
     gene            10197..10616
                     /gene="rpsL"
                     /locus_tag="VIN32_04000"
     CDS             10197..10616
                     /gene="rpsL"
                     /locus_tag="VIN32_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEY8921776.1"
                     /translation="MPTISQLVRKGRKPAGQKVKAPALRRTFNTLKQTTSEGRGAPQR
                     RGVCLQVMTRTPKKPNSALRKVARVRLTNGMEVTAYIPGVGHNLQEHSVVLVRGGRVK
                     DLPAVKYHIVRGTLDAAGVRDRRQSRSKYGAKSPGKG"
     gene            10624..11097
                     /gene="rpsG"
                     /locus_tag="VIN32_04005"
     CDS             10624..11097
                     /gene="rpsG"
                     /locus_tag="VIN32_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012995917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEY8921777.1"
                     /translation="MPRRPTIARKTKYQQVLVGTALTLDQFTNKLMHGGKRQLAARIL
                     EDALARCEKQAGKSGVEVLELALRNAMPLIEVKPKRVGGSTYQIPVEVRADRRISLGM
                     RWLIDSARKRNGHSMGEKLAAEFVDASNGIGNAVKRREETHRMAEANKAFSHFKW"
     gene            11105..13180
                     /gene="fusA"
                     /locus_tag="VIN32_04010"
     CDS             11105..13180
                     /gene="fusA"
                     /locus_tag="VIN32_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEY8921778.1"
                     /translation="MAQAYPLQKTRNIGIIAHIDAGKTTVSERILFYTKKIYKLGEVH
                     EGAATMDWMEQEQERGITITAAATTCFWRDHRINLIDTPGHVDFTVEVERSLRVLDGA
                     VVVFDGVAGVEPQSETVWRQADRYRVPRFCFVNKLDRTGADFWRVVDMIRERLGATAV
                     PVQLPIGTEGGFRGVIDLLEMQAITYGNDLGDLVEVGEIPAELAEDAAKRRDKLVEAV
                     ADLDDDIAHRFLEGQDVDADALRRALRAGTLASKIIPVLCGSALKNKGVQPMLDAVIE
                     YLPSPLDVAAVIGTDPRSGEQVTRHADPAEPFAALAFKIAADPFVGKLAFFRVYSGTL
                     KTGSYVYNATKGEKERIGRLIELHANHREEIESVSAGDIAAAVGLKTTFTGDTLCDES
                     KPVILEAITFPEPVIELAVEPKTKADQDKMAIALQRLGEEDPTFRVSSDEESGQTRIA
                     GMGELHLEVIVDRMLREFKVQANVGRPQVSYRETIRKPVKAEGRFVRQTGGKGQYGHA
                     VLIMEPLERGGGVVFESKVVGGAVPREFWKPIEQGVRDALAGGVIAGFPVIDLKATLI
                     DGSFHEVDSSEMAFKIAGSMAAKDGVAKADPAILEPVMKVEVVAPEEYMGDVIGNLNS
                     KRGHMDGIDERGSSRVIRAHVPLAEMFGYATELRSMTQGRATYSMEFSRYSEVPTNLA
                     NELIAKSKS"
     gene            13202..14404
                     /gene="tuf"
                     /locus_tag="VIN32_04015"
     CDS             13202..14404
                     /gene="tuf"
                     /locus_tag="VIN32_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005385492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEY8921779.1"
                     /translation="MAKQKFDRSKPHVNVGTIGHVDHGKTTLTAAITKYLAMQGGADF
                     RAFDSIDNAPEERQRGITIAISHVEYQTAARHYAHVDCPGHADYIKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLARQVEVPSLVVYLNKVDAVDDEMLIDLVEMEVRELLTK
                     YDFPGDETPVIRGSALKALESTSTDQDAPEYASIKELMNAVDTYIPTPVREVDKPFLM
                     PIEDVFGIKGRGTVATGRIERGVVHTSDTVELVGIHAKSRQLVVTGVEMFKKTLDEGQ
                     AGDNVGLLLRGIEREELERGQVMAKPNSVKPGTKFKAEVLVLTKEEGGRHTPFYAGYR
                     PQFYLRTTDVTGAISMPPGVEMIMPGDNVNIDVELITPIAIEQGQRFAIREGGHTVGA
                     GVITALVG"
     gene            14412..14720
                     /gene="rpsJ"
                     /locus_tag="VIN32_04020"
     CDS             14412..14720
                     /gene="rpsJ"
                     /locus_tag="VIN32_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEY8921780.1"
                     /translation="MAKQRIRIRLKAYDHKLLDQSAEQIVETAQRTGAAIAGPVPLPT
                     RIEKYTVIRGPFVHKDGREQFEIRTHKRLIDIIDPSTKTVDALMRLSLAAGVDIEIKM
                     "
     gene            14740..15363
                     /gene="rplC"
                     /locus_tag="VIN32_04025"
     CDS             14740..15363
                     /gene="rplC"
                     /locus_tag="VIN32_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEY8921781.1"
                     /translation="MPAGLIGRKLGMTRIFGDDGSVVPVSVIEATPNNVTRLRTTERD
                     GYAALQVGAGSAKRQSKPVAGQFKELAKELQRPRHVREFRVESTEGYELGQQVDVNLF
                     APGDLVDVTGVSKGHGFTGTIARHHFKRGPKTHGSTNIRQPGSIGAGTTPGRVFRGTR
                     MGGHMGDDRVTVKKMQVVKVDPERNLILVKGAVPGARNAILLVQKAK"
     gene            15366..15989
                     /gene="rplD"
                     /locus_tag="VIN32_04030"
     CDS             15366..15989
                     /gene="rplD"
                     /locus_tag="VIN32_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013259728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEY8921782.1"
                     /translation="MEQAPILTVSGSESGKVDLVPSLFAAPVNEALIHQAIVRQLAGQ
                     RLGNADTLTRGQVRGGGKKPWRQKGTGRARQGTRSAPQWKGGGVVFGPHPRGYEQRMP
                     AKMRRAALQGVLSGKADDGALRVVEGFGLETMGTKAFAERLVAWKTEGKVLVVLPARD
                     PVVERSCRNLREVRVLLADSLNVVDLLEADTIVFTADALARAQEVYG"
     gene            15986..16282
                     /gene="rplW"
                     /locus_tag="VIN32_04035"
     CDS             15986..16282
                     /gene="rplW"
                     /locus_tag="VIN32_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEY8921783.1"
                     /translation="MTAVSVHDILIRPVISEKSVAQTERNNYTFAVGRDANKFQIKDA
                     VEAEFKVTVLGVRVLWVKPKQKRRGRQTMGTVPGWRKAVVTIAAGQKIELFEAV"
     gene            16287..17117
                     /gene="rplB"
                     /locus_tag="VIN32_04040"
     CDS             16287..17117
                     /gene="rplB"
                     /locus_tag="VIN32_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEY8921784.1"
                     /translation="MPLRNYRPTSPGLRQMTRSTFDEITTDEPHKPLTERLVRKAGRN
                     SQGRLTVRHQGAGHKRLYRVIDWKRDKFGVPATIATVEYDPNRSARIGLLHYADGDKR
                     YMLLPHGLGVGDVVVSGRDAEARVGNALPLARMPLGTQIHNVEMVAGRGGQIVRSAGS
                     SAQLLAKEGEMATIRLPSGEVRRVRADCMATVGQVSNLDHENQNIGKAGRSRHMGRRP
                     EVRGVVMNPRDHPHGGGEGKSPTGMPPKTPWGKPAMGLRTRKNKTTGKMIVRRRYGRG
                     "
     gene            17127..17411
                     /gene="rpsS"
                     /locus_tag="VIN32_04045"
     CDS             17127..17411
                     /gene="rpsS"
                     /locus_tag="VIN32_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEY8921785.1"
                     /translation="MSRSVKKGPFVDARLLGRIDEMNKHNEHKVLKTWSRPSVIFPQM
                     IGHTIAVHDGKRHVPVFVTENMVGHRLGEFAPTRHYRGHGKATDRSTALK"
     gene            17423..17764
                     /gene="rplV"
                     /locus_tag="VIN32_04050"
     CDS             17423..17764
                     /gene="rplV"
                     /locus_tag="VIN32_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015043937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEY8921786.1"
                     /translation="MRVIATAKYIRISPRKVRLVTDLITGKPVEEAAAILRFLPNAAA
                     RDVAKVLKSATANAENNFNLSADELHVLSATANEGPTLKRFRPRAQGRTFSILKRTSH
                     ITVAVADREES"
     gene            17764..18384
                     /gene="rpsC"
                     /locus_tag="VIN32_04055"
                     /pseudo
     CDS             17764..18384
                     /gene="rpsC"
                     /locus_tag="VIN32_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683987.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
     gene            18535..18948
                     /gene="rplP"
                     /locus_tag="VIN32_04060"
     CDS             18535..18948
                     /gene="rplP"
                     /locus_tag="VIN32_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005398634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEY8921787.1"
                     /translation="MLMPKRVTHRKVQRGRRAGMAKGGTTISFCEYGLMAEEVCWLTS
                     RQIEAARRAMTHHIKRGGRIWIRVFPDKPVTKKPAEVRMGSGKGAPDHWVAVVKPGRV
                     MFEMSGVPEDIAREAMRLASHKLPIQTRFVAKEGA"
     gene            18951..19178
                     /gene="rpmC"
                     /locus_tag="VIN32_04065"
     CDS             18951..19178
                     /gene="rpmC"
                     /locus_tag="VIN32_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015780011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEY8921788.1"
                     /translation="MDINEIRGLSDLDLESALVDAKQELWKIRFDLATRQEKNYSRLP
                     ATRKRIARILTVMTERQHAADDAAAAVGKAS"
     gene            19178..19453
                     /gene="rpsQ"
                     /locus_tag="VIN32_04070"
     CDS             19178..19453
                     /gene="rpsQ"
                     /locus_tag="VIN32_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEY8921789.1"
                     /translation="MAGKRRTKVGRVISDKMDKTVVVSVERLRRHPIYKRVVRLSSKF
                     KAHDEENSAHVGDTVLIEESKPLSREKRWRVVEVVARGSGKEMVDTA"
     gene            19450..19818
                     /gene="rplN"
                     /locus_tag="VIN32_04075"
     CDS             19450..19818
                     /gene="rplN"
                     /locus_tag="VIN32_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEY8921790.1"
                     /translation="MIRQYTRLRVADNTGARELMCITVIGRSSSDTAEVGDVIVASVK
                     QALPNTGVKKGDVVRAVIVRTAKEYGRPDGSHIRFDENAAVLITPQGSPRGTRIFGPV
                     ARELRERNYMKIVSLAPEVL"
     gene            19820..20149
                     /gene="rplX"
                     /locus_tag="VIN32_04080"
     CDS             19820..20149
                     /gene="rplX"
                     /locus_tag="VIN32_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEY8921791.1"
                     /translation="MNIRKGDTVLVLAGKDRGKRGVVERIERTKRGLGVVVPGLNMAK
                     RHQRPRTRTQQAGILDLPVPLHLSNVQVVCPRCDRPTRVSHELLEGGSKHVRVCKHCG
                     EQMEVAQ"
     gene            20146..20817
                     /gene="rplE"
                     /locus_tag="VIN32_04085"
     CDS             20146..20817
                     /gene="rplE"
                     /locus_tag="VIN32_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEY8921792.1"
                     /translation="MSDEEATPKAAPKAPRRSPRRPAAATPAAQPTAQPAESGPAAET
                     GGLRRRYLDEVAPAMRREFGYANLMQIPRLEKVVVNIGLGEAIQNAKALDAAVGDLTQ
                     ITGQKPIVTRAKRSIAQFRLRTGMPIGAKVTLRGQRMYDFLERTMRLALPRIRDFRGI
                     SRNSFDGRGNFSLGLREQLVYPEIDYDKIDRLRGMEISVVTTAKTDEEGLKLLQLLGM
                     PFQAS"
     gene            20829..21014
                     /locus_tag="VIN32_04090"
     CDS             20829..21014
                     /locus_tag="VIN32_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010936264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HEY8921793.1"
                     /translation="MAKKSMIVKSQRKPRFAVQHKSRCAVCGRPRGYMRRFALCRICF
                     RERALEGQLPGVTKSSW"
BASE COUNT         3937 a         7050 c         7119 g         3021 t
ORIGIN      
        1 gggcctggta gtcaggtcgc tggccgcgcg gctggggatc cgggggcccg atcaggatca
       61 ccttcaggcc ggccttgcga gctgccccta cctggcgcgg ccgatcggta agcagccagg
      121 tgccctcctg ggcgtcccgc acctcgcccc atcgctcgac gccatggccg tcctggctga
      181 ggcgacgatc ggcggtcggg tcctggaagt cgaccacgcg atatgcccct ctcccgatgg
      241 aggctcggac ccacgcctcc cgctcccgcg catccgatgg caggtcacga ccggcgacct
      301 gcctacccac caggatgatg ggccggccga tccagccgat ccttcgcagg gcaaccggcg
      361 cctcgtcgag ggctacgggc tgatcggcgc gctgcacgat cagcgcatcg agcgcgatga
      421 ggatccccgc cgcgggctgg cctgccgccg caggatcagg gggcatgacc gcctaccgct
      481 cgggcgtcgc ccacctctgc accgtcacga ggcatctcga gagggggagt ctcgtccacg
      541 ttcaggacgt agccgatccc ctggaaggtt gtaatcgggc ctggctcgcc gggcaaggct
      601 cccagcttgc gacgcacacg actgatccac acccgcaggt actgcaggtc gtcgcggtat
      661 tcggggcccc acaccttggt gaggagttcc gtgtgcagca cgaccttgcc agcattggcc
      721 gccaggtgct ggagcaacag ccattccgtc ctgctcagct gcacgagctg gccgttgcgg
      781 ctcatcatcc gccgctccag gtcgatctcg acctcgccga tgtggaggat cccgcccgcc
      841 ggtgtgatgc ccgccgcgcg ccgcagcacc gcccgaacac gcgccgccag ctcgtccgga
      901 tggaagggct tggcgatgta gtcgtccgca cccaggtcca gccccttcgc cttgtccgag
      961 gtcgagccct ttgcggtaag caggatgacc ggcaccggcc gccattcacg gagctggcgc
     1021 atgacctcga tcccgtccag gtccggcatc accacgtcga gcaggacgat gtccgggcgc
     1081 atctcctcgg ccttgcggag cgcctcctcc ccgccgccgg cggtgacgac tcgaaagccc
     1141 tcatcggcca ggctcatggc gacgagcttc gtgattcggg gctcatcgtc cgcgacgagc
     1201 acgagtggct ggttgttcat gcgggctcct caccctgggc atgcgaacgg tcagggtcca
     1261 cgatcgagca tcgtacaagt cctgtgctca cgcggatcgt ggggccgcta cgactcgcgc
     1321 cgggcgatcc cgatcagggc gatgatcaga acctccagga tcgtccagcc gatgagtgcg
     1381 acgagcgcag cgatgtcgaa gattgatgct cccgctcgga cggcgttggt gttcaggatc
     1441 ccctcgaacg ggccgacgaa gaatccgctg gcgtcgtaga tgaagcggac cagctggttc
     1501 gcctggttcg catcggccaa cagcagcacg attctgatca cgatcagcgc ctggatgatc
     1561 ccgaagatga agacgaccac ccgtcgagct acctcggcac cgctcggcga gcgtcggacc
     1621 gtctccgaca cgaccgatga gttgctgacg gtctcgttcg gataccgcgc cggttcgacg
     1681 accggttgca ctggttcgac ggacgtcgtg gtatctgccg gtggggtcgt cacgctctgg
     1741 cgttcgacca cgcgctcttc gtccatgatg agcacctcct ggttgggtcc agcgtccatc
     1801 atgtcggtgc cccggccgca gtgagccctc ctcctggggg agaagtgttg caatctcgtt
     1861 gcgcggcata ggaggcccgc ggactggggc cagcgggatc cctaccgctt cacttgacga
     1921 agcgcatcgg cttgatctaa actcctcgtt cgcgtgtccg cgccccaccg tgtcctcttc
     1981 gaggagtcct gaatggcatt ggccgagtca gccagccgat ttgagttggc cccgattcgt
     2041 gaaaactttt cacatattcc cgaggtacta cccctcccga acctgatcca gacccagacc
     2101 gagtccttca attggttcgt ccagaccggc ctccgcgagc tgctggacga gatcaccccg
     2161 atcaccgact tcaccggcaa gaacctcgag ctgcatttcc gcgactacta ctttgaagag
     2221 ccgaagttcg acgaggacga gtgccgtact cgtgatctga cctactccaa gccgctcaag
     2281 gtcgaggtcg acctgatcgt caaggagacc gacgaggtca agcaccagac cgtcttcatg
     2341 ggcgacttcc cgtggatgac gcagcagggt acgttcatca tcaacggcgc cgagcgcgtg
     2401 gtcgtatcgc agctcgttcg ctcgccgggc gtctactaca ccggatccga ggatccggcg
     2461 accgggcgca tcctcttcgc cgccaaggtg atcccgaacc gtggcgcctg gctcgagttc
     2521 gagaccagca acaaggacat gctcagcgtc aaggtggacc gcaagcggaa gatccccgtc
     2581 accacgctgc tgcgcgcggt gggcgtcgag gacaacgccg agatgctccg caccttcggt
     2641 ccggccgatg accatcccga gcacccgtac gtctcgctca cgctcgacaa ggatcctgcc
     2701 gcgacgcagg acgaggccct gatcgaggtc tacaagaagc tgcgacccgg cgatccgccg
     2761 acggtcgaca acgcacgcgc cctggtccag agcctcttct tcaacttccg ccgctatgac
     2821 ctcggccgcg tcggccgcta caagctcaac aaggcactcg gcgacgtcgc cgccaagctg
     2881 aagatcgcct tgcccgccga gggcggcgag accacgcgcg agtcgcgggt catcagccag
     2941 aacgacatcg tggcgatcat cgggaagctg atcgagctga acaacggccg aggcacgggc
     3001 gacgacatcg accaccttgg caatcgccgc atccgcgcca acggtgagtt gatccagaac
     3061 cagttccgca tcggcctgct tcgcatggag cgggtcgtca aggagcggat gacgatcacc
     3121 gatgccgagc aggccacgcc gaactcgctc atcaacatcc gcccggtggt ggcggcgatg
     3181 aaggagttct tcgggggcag ccagctgtcg cagttcatgg accagaccaa tccgctggcg
     3241 gagctgacca acaagcgccg cctctccgcg ctcggcccgg gtggcctgtc acgcgagcgc
     3301 gccgggttcg acgtccgcga cgttcacccg tcgcattacg gccgcatgtg ccccatcgag
     3361 accccggagg gaccgaacat cggtctcatc ggctcgctgg ccacctacgg caagatcaac
     3421 gagcacggct tcatcgagac gccgtaccgc gaggtgcgca agttcgtctc ccatcgggac
     3481 aagcaggccg atgtggtcgg agagacgctg gccgaggatc tcctggtcgg ggagcgcggc
     3541 aagaagctgg ccaaggccgg cgccccgatc gacgagaagc tgtgggcggc gatcaagggg
     3601 aacaagctgg catcggtccg catcgttccg gtggtcaccg cgcagatcaa gtacctcgcc
     3661 gccgacgagg aggagaagtt ctacatcgcc caggccaacg cgccgctcga tgaggggaac
     3721 cacttcgtcg aggagcgcgt aatggtccgc taccgcgacg agttccacgt ccaggtcaat
     3781 gaggacgtcg actacatgga cgtcagcccc aagcagatcg tcagcgtggc gaccgccatg
     3841 atccccttcc ttgagcacga tgacgccaac cgagcgctga tgggctcgaa catgatgcgc
     3901 cagtcggtcc cgctgcttca gcccgaggcc ccggtggtcg ggacgggcgt cgagtaccgg
     3961 gccgccttcg acagccagca ggtggtcgtc gcggaagcgg gcggcacggt ggtgagcgtc
     4021 acctcggccg acatccaggt ggagcgggac gagggtgacg tgcgcgacac gtaccacctc
     4081 aagaagttcc tgcgcagcaa ccaggggacc tgcatcaacc agcgaccgat cgtcgacgtc
     4141 ggcgaccggg tggcggcggg acaggtcctg gccgactcgt cgagcaccga caagggcgag
     4201 atggcgcttg gccagaacat cctggttgcg ttcatgccct gggagggcgg caactacgag
     4261 gatgcgatcg tgatcagcga gcggctcgtc cgcgaggacc tgttcacctc gatccacatc
     4321 gagaagcacg agatcgaggc acgcgacacg aagctgggcc ccgaggagat cacccgcgac
     4381 atcccgaacg tcggcgagga gagccttcgc aacctcgacg aggacgggat catctacgag
     4441 ggggccgagg tcgctcccgg cgacatcctg gtcggcaaga tcacgcccaa gggcgagacg
     4501 gagctgacgg ctgaggagcg cctgctgcgg gcgatcttcg gtgagaaggc ccgcgaggtg
     4561 aaggacagct cgctgcgact cccccacggg gagcgcggca ccgtggtcaa cgtgcggcag
     4621 ttcagccgcg agagtggcga cgagctcctt cccggcgtca accggctggt ccgggtcagc
     4681 gtggcccaga agcgcaagat cgccgtcggc gacaagatgg ccggacgcca tggcaacaag
     4741 ggcgtcatcg ccatgatcct gccggtcgag gacatgcccc tcatgccgga cgggacgccg
     4801 gtcgacatcg tcctcaaccc gctcggggta ccgagccgga tgaacatcgg ccagatcctg
     4861 gagacgcacc tgggttgggc gatgaaggtg ctcggcctga aggccgcgac gccggtcttc
     4921 gacggggcga ccgaggcgca gatcacggca tcgctgaaga aggcggccgc ggtcaagggt
     4981 tccgtcgtga gcgaggacgg aaagacggtc ctgcgcgacg gacgcactgg tgaggcgttc
     5041 gaccaccgcg tgacggtcgg ctacatctac atgctcaagc tccaccacct ggtggaggac
     5101 aagatccacg cccggagcac cggcccctac tcgctcatca cccagcagcc gctgggtggc
     5161 aaggcgcagt tcggcggcca gcgcttcggc gagatggagg tctgggcgct ggaagcctac
     5221 ggcgcggcga acatcctcca ggagctgctc accgtcaaga gcgacgatgt gatgggccgc
     5281 gtgcagacgt acgaggcgat cgtcaaggga gaggagatcc agcctccggg cgttccggag
     5341 tccttcaagg tgttgatcaa ggagcttcag gcgctcggcc tgtcggtcga gatcctgaac
     5401 gagaacgagg aagagatccg cttcatcgag gacaccggca gttacccgct gccggacctg
     5461 ggtgggatca atctccaggg atttgaggaa tagcccgagg agcggcgtcg tgacggcgtc
     5521 gcgcctcacc tatcggtcca tttcgagccg gggcccggtc agccctggcg atccgcgcca
     5581 gaagcggcgt cagccgcagg agccgtaagc cacccatgct ggaagtcaac aacttcgacg
     5641 cgatccgcat cagcctcgcc tcgcccgatc agatcaagag ctggtcttcg ggtgaggtca
     5701 ccaaaccgga gacgatcaac taccgcaccc tcaagcccga gaaggacggt ctcttcgacg
     5761 agcgcatctt cggtcctacg aaggactggg agtgctattg cggcaagtac aagcgcattc
     5821 gctacaaggg gatcatctgc gacaagtgtg gcgtcgaggt cacccgcagc aaggtccgtc
     5881 gcgagcggat ggggcacatc aagctcgcca gtcccgtgag ccacatctgg tacttcaagg
     5941 gcacgccgtc gcggctgggg atcctgctcg acgtcagccc gcgcaacctg gagcggatcc
     6001 tgtacttcgc gctctacgtg gtctctcggg tcgacgagca cgagcgtgag aaggcgctcg
     6061 cacgggtcga ggaagagatg gccgagcgca tcggtcgcgc cggcgagctg gtcgaggcca
     6121 agaaggccga gctcgaggcg gcgatcgccg acaagcgaac gcaggtcacc ggcgacgcgg
     6181 aggccgatgc ggcccgcgcc gatgggctga tcgccacccg cagcgaggag ctggccgccg
     6241 ccggccagca ggtcgaggct gccctcaagg agggcggcaa gaccgtcgcg gaggacatcg
     6301 ttttcacccc caccggcgag gtggtggcac gcgccggaga ggcctccaag ggcgccctgg
     6361 tcgcgctgcg caaggccgtc aaggccgagg tcgacgccct cgagaaggag gtcaaggagg
     6421 ccaaggccca cgccgccgag cgcctgcagg ccgccatcgc cgacctgagc agcggtatcg
     6481 acgacgccct ggccagcgag aaggcccagg tcgcccgcga gaccgatgac gcacgcctcg
     6541 cggcgcgcgc tgcgcgtgcc cagatcgccg acatcaaggt catgcagacc ctgaccgagt
     6601 ccgagtaccg ctcgctgtct gagcagtacg gcaggctctt cgacgccggc atgggtgccg
     6661 aggcgatcaa gaagatcatc gagcagctcg acctggacga cctcgcccag cgactgcacg
     6721 tcgagatgcg ccagacctcc gggcagcgac gcaagaaggc gatcaagcgc cttcggctga
     6781 tcgaggcctt ccgcaagagc ggcgcgcggc ccgagtggat gatcctgtct accctgccgg
     6841 tcatcccgcc cgacctgcgc ccgatggtgc agctcgacgg cggacggttc gcgaccagcg
     6901 acctcaacga cctctaccgg cgcgtcatca accgcaacaa ccgcctcagc cgcctcctcg
     6961 acctcgaggc gccggagatc atcatccgca atgagaagcg gatgctgcag gaggcatgcg
     7021 acgcgctgat cgacaacggt cggcgggggc gtgccatcgc aggaaccggc aaccatcggc
     7081 tcaagagcct ttcggacatg ctcaagggca agcagggacg cttccggcag aacctgctcg
     7141 gcaagcgcgt cgactactcc ggccgctcgg tgatcgtggt cggcccggag ctgaagctgc
     7201 accagtgcgg gctccccaag aagatggcgc tcgagctgtt caagccgttc gtgatgcgcc
     7261 agctggtcga gaagggcttc gcccacaaca tcaagagcgc caagcggatc gtcgagcgcg
     7321 tgcgaccgga ggtctgggac gtcctcgaag aggtgatcca ggaccatccg gtgctgctca
     7381 accgggcccc gacgctgcat cggctcggga tccaggcgtt catgccggtg ctggtggagg
     7441 gatccgcgat ccagatccac ccgctcgtct gcttcgcctt caacgccgac ttcgacggag
     7501 accagatggc ggtccacgtg ccgctctcga ccgcggcgca gcgcgaggcg aaggagctga
     7561 tgctctcgac ccagaacctg ctctccgcgg ccgatggctc gccggtcgtg tcgccgacgc
     7621 atgacatggt gctcggctgc tactacctga ccatcgagga gccgaccgag aagggtgccg
     7681 gcaaggcgtt tgcgagtgcc aacgaggccg tcatggtcac ccagctcggc cacgtgcacg
     7741 tccaggcccc catcaaggtg gagcttgagg tctgggacgc ggccgccaac gatggcgctg
     7801 gggagatgcg cacggagctc gtcgagacga ccgccggccg cgtgatcttc aacacggtca
     7861 tccccaagga gctcggcttc gtcaatcggg agatggatcg caaggggctg aaggcgctca
     7921 tcgcgcagtg ctaccgcgag ctcggtccgg ccgcaaccac ccagctcgtg gacggcatca
     7981 agacgctcgg cttccactac gccacgatgg cgggcatcac gatcggcatc ggggacatcc
     8041 gggtgccgac cgagaaggtc aagctgcttg ccggagccga cgccaaggtg actgagatcg
     8101 accgggaatt ccgacgcggc ttcatcaccg aggacgagcg ctacaaccaa acggtggatg
     8161 tctggcgcgg gacgaccgac gccgtgaccg agaagatgct ggccggcctc gacaagcgtg
     8221 gctctgtctg gatgatcacg cactcgggtg cccgcggcaa cgtgacccag gtgcaccagc
     8281 tggctggcat gcgcgggctg atggccgacc cctcgggccg aatcatcgac ctgcccatcc
     8341 gcagcaacct gcgcgaaggg atgagcgtgc tcgagtactt catctccagc cacggggctc
     8401 gcaagggcct cgccgacacc gccctgcgca ccgccgactc tgggtacctg acccgccgcc
     8461 tggtggacgt cgcgcaggac gtgatcacgc gcgaggacga ctgcggcacc gacctcggga
     8521 cgtggatcac ccgcgaggag tcgacccgga tccccgagcc gttcgtcgac cgcctggtcg
     8581 gccgcatggc cgcactggac ctcgtggacg agaaggccgg ccaacagatc gtggcgcgca
     8641 acgatgagat cgacgaggtc gccatcgagc ggatcgatgc cctcgggatc gatcgggtca
     8701 gtgtccgatc gcctctgacc tgtgcggcgc gccacggcgt atgccgcatg tgctacggac
     8761 gcaacctcgc gaccggcaac ctcgtcgacc ttggccaggc ggtggggatc atcgcagccc
     8821 agtcgatcgg ggagccgggg acccagctca ccatgcggac cttccacacc ggcggcgtcg
     8881 ccggcgagga catcacccag ggcctgccac gcgtcgagga gctgttcgag gctcgtatcc
     8941 ccaaggggca ggcgatcatg accgagatcg acgggacgat cgaggtcgtc cgcgcaggga
     9001 cggaccagcc ggtcgtggtg cgcgtcacgc atcgagacac ctacgacacg ccgatcaagc
     9061 tcaccccgca gcacgagctg ctggtggcga acggcgatga ggtggccgac ggccagctgc
     9121 tggcccgcat cggtgagggt gccgacgcgg tcgaggtgaa gtcaccggcc gccggtcggg
     9181 tcaccaagaa ggggagcacc ctgacgctgc actccgagga catcgacacc cgggaatacc
     9241 aggtgccgca ctcagcccgc ctccgggtcg agaccggcga ggaggtgctg gccggccacc
     9301 cgctgaccga gggctcgctg aaccccaagg accttctgct gatcaagggc gtggacaccg
     9361 tccagcgctt cctggtggcc gaggtccaga cggtataccg cagccagggt gtcacgatcg
     9421 ccgacaagca catcgagatc atcgtgcgtc agatgctgcg caaggtgacc gtggacgcgg
     9481 ctggcgacac ggagctgctc cccagcgagc tgatcgaccg cttcgagttc gaggaggtca
     9541 acaaccggat cctggcggag ggcggcgagc cggcgacggc gcagaccgtt ctgctgggcg
     9601 tcaccaaggc gtcgctgaac accagctcgt tcctcgcggc cgcatccttc caggagacga
     9661 cgcgggtcct caccgaggcc gccatcaacg gctccaagga ccacctgctg gggctcaagg
     9721 agaatgtgat catcggcaag ctcatcccgg cagggaccgg cgccgagtcg cgggcggctg
     9781 ccgctgccaa ggcgcgcgag ctttcgcccg aggaggccga gtcgctccgc gagcgcgagg
     9841 cggcgatgac cttcctgacc ggcgacgagg aggtcgatgg cgacggttcg tccgcgaccg
     9901 acggagtcga gccggcggcc gaagaggcgc ccgcagcggt cgtcgaagag ggctgatccg
     9961 gaggcccgtc atgggctcga cgcaaggcgg ccaaacgagg cgggcaaccg cctcgtttgg
    10021 tttgacactc gtgcggcgcg aaccgtatac tccgtgttcg gtgtcccgct cgcgggacgc
    10081 cttcgtgtgt gccctgcggg agggcgccgt cagccccttg agtgccggcc ccggttcggg
    10141 tcgccatcac tcgatgccga cgcacaacca atcccacgga gtgtgaggtt cttttcttgc
    10201 cgacgatcag ccagctggtc cgcaagggcc gcaagccggc cgggcagaag gtgaaggcac
    10261 cggcgctgcg ccgtaccttc aacacgctga agcagacgac ctccgaggga cgcggcgccc
    10321 cgcagcgccg cggcgtgtgc ctgcaggtca tgacccgcac cccaaagaag ccgaactccg
    10381 ccctgcgcaa ggtcgcgcgc gtgcgcctca ccaatggcat ggaggtcacg gcatacatcc
    10441 ccggggtcgg ccacaacctc caggagcact ccgtggtgct ggtccgcggc gggcgggtca
    10501 aggacctccc ggccgtgaag taccacatcg ttcgcggcac gctggacgcc gccggcgtcc
    10561 gcgatcgacg ccagagccgc tccaagtacg gcgccaagtc gccgggcaag ggatagccgg
    10621 ccgatgcccc gccgtccgac catcgcgcgc aagaccaagt accagcaagt gctggtcggc
    10681 accgcgctga ccctcgacca gttcaccaac aagctgatgc acggtggcaa gcgccagctg
    10741 gccgcgcgca tcctcgagga cgcgctggcc cgctgcgaga agcaggccgg gaagtcgggg
    10801 gtcgaggtgc tggagctggc cctgcgcaac gcgatgcccc tgatcgaggt caagcccaag
    10861 cgcgttggtg gctcgaccta ccagatcccg gtcgaggtgc gggcggatcg ccgcatcagc
    10921 ctcggcatgc ggtggctgat cgactctgcc cggaagcgga acgggcacag catgggggag
    10981 aagctggctg cggagttcgt ggatgcctcg aacggtatcg gcaacgccgt caagcgccgc
    11041 gaggagacgc atcggatggc ggaggccaac aaggccttca gccacttcaa gtggtaggcc
    11101 gatgatggcc caggcatacc cactccagaa gacccgcaac atcgggatca tcgcgcacat
    11161 cgacgccggc aagacgaccg tctcggagcg gatcctcttc tataccaaga agatctacaa
    11221 gctcggcgag gtccacgagg gcgctgccac gatggactgg atggagcagg agcaggagcg
    11281 cggtatcacg atcacggccg ctgccacgac ctgcttctgg cgggatcatc gtatcaactt
    11341 gattgacacg cccggccacg tggatttcac ggtggaggtc gagcgcagcc tgcgcgtgct
    11401 cgacggggcg gtggtcgtgt tcgacggcgt ggcaggcgtt gagccgcagt cggaaacggt
    11461 ctggcgccag gcggatcgct accgggtgcc gcgcttctgc ttcgtcaaca agctcgaccg
    11521 caccggtgct gacttctggc gcgtcgtcga catgatccgc gaacggctcg gcgccaccgc
    11581 cgtcccggtg cagctgccga tcgggaccga gggcggcttc cgcggcgtga tcgacctgct
    11641 cgagatgcag gcgatcacct acggcaacga cctgggcgac ctggtcgagg tcggcgagat
    11701 cccggccgag ctggccgaag acgcggccaa aaggcgggac aagctggtcg aggcggtcgc
    11761 cgacctcgac gacgacatcg cgcatcgctt cctggagggc caggacgtcg acgccgatgc
    11821 cctgcgccgc gccctgcgcg ccggcacgct ggcatcgaag atcatcccgg tcctgtgcgg
    11881 ctccgccctc aagaacaagg gcgtccagcc gatgctcgac gcggtgatcg agtacctccc
    11941 ctcgccgctc gacgtggcgg cagtcatcgg caccgacccg cgcagcggcg agcaggtgac
    12001 ccgccatgcg gacccggccg agccattcgc ggcccttgcc ttcaagatcg ccgccgaccc
    12061 gttcgtcggc aagctggcat tcttccgcgt ctactccggc accctcaaga ccggctcgta
    12121 cgtgtacaac gcgaccaagg gcgagaagga gcggatcggc cggctgatcg agctgcacgc
    12181 caaccatcgc gaggagatcg agtctgtcag cgccggcgac atcgccgccg ctgtgggcct
    12241 caagacgacc ttcaccggcg acacgctctg cgacgagtcg aagccggtca tcctcgaggc
    12301 gatcaccttc cccgagccgg tcatcgagct cgccgtcgag cccaagacca aggccgacca
    12361 ggacaagatg gcgatcgccc tgcagcgcct cggggaggag gacccgacct tccgcgtcag
    12421 cagtgacgag gagtcgggcc agacccgcat cgcgggcatg ggcgagctgc acctcgaggt
    12481 gatcgtcgac cgcatgctgc gcgagttcaa ggtgcaggcc aacgtcggcc gcccgcaggt
    12541 gagctaccgc gagacgatcc gcaagcccgt caaggcggag ggtcgcttcg tgcgccagac
    12601 cggcggcaag ggccagtacg gccacgccgt gctgatcatg gagccgctcg agcgtggcgg
    12661 cggcgtggtc ttcgaatcga aggtcgtcgg cggggcggtg ccgcgcgagt tctggaagcc
    12721 gatcgagcag ggggtccgtg atgccctcgc cggcggcgtg atcgccggct ttccggtgat
    12781 cgacctcaag gcgaccctca tcgacgggtc gttccacgag gtcgactcct cggagatggc
    12841 gttcaagatc gccggatcga tggccgcaaa ggacggtgtc gcaaaggccg atcccgcgat
    12901 cctggagccg gtcatgaagg tcgaggtggt cgcccccgag gagtacatgg gcgacgtcat
    12961 cggcaacctg aacagcaagc ggggacacat ggatggcatc gacgagcgag gcagctcgcg
    13021 cgtcatccgt gcccatgtac cactggccga gatgttcggc tacgccacgg agctgcgcag
    13081 catgacccag gggcgtgcca cctattcgat ggagttctcc cgctactcag aagtcccaac
    13141 caacctcgcc aacgagctga tcgcgaagtc caagagctaa aggagcaacg aaggacacac
    13201 catggcaaag cagaagttcg accgcagcaa gccgcacgtc aacgtcggga ccatcggtca
    13261 tgtcgaccac ggcaagacga ccctgaccgc ggcgatcacc aagtatctgg ccatgcaggg
    13321 cggtgccgac ttccgcgcat tcgactcgat cgataacgcc ccggaagagc ggcagcgcgg
    13381 catcaccatc gcgatcagcc acgtggagta ccagacggcg gcgcggcact acgcccacgt
    13441 cgactgcccg ggccacgcgg actacatcaa gaacatgatc accggcgcgg cgcagatgga
    13501 cggcgcgatc ctggtcgtct cggccgctga cggcccgatg ccccagacgc gcgagcacat
    13561 cctgctcgcc cgccaggtcg aggtcccgtc gctggtcgtg tacctcaaca aggtcgacgc
    13621 ggtcgacgac gagatgctga tcgacctggt cgagatggag gtccgcgagc tcctgaccaa
    13681 gtacgacttc cccggagacg agaccccggt catccgcggc tcggcgctca aggcgctgga
    13741 gtcgacctcg accgaccagg acgcgccgga gtacgcctcc atcaaggagc tgatgaatgc
    13801 ggtcgacacc tacatcccga ccccggtccg cgaggtcgac aagccgttcc tgatgccgat
    13861 cgaggacgtc ttcgggatca agggccgcgg caccgtggca accgggcgga tcgagcgtgg
    13921 cgtggtccac accagcgaca cggtggagct ggtcgggatc cacgccaaga gccgccagtt
    13981 ggtggtcacc ggcgtcgaga tgttcaagaa gaccctcgac gagggacagg ccggcgacaa
    14041 cgtcgggctc ctgctgcgag ggatcgagcg cgaggagctc gagcgcggcc aggtgatggc
    14101 caagccgaac agcgtcaagc ccggcaccaa gttcaaggcc gaggtgctcg tcctcaccaa
    14161 ggaggagggc ggccgtcaca cgccgttcta cgccggctac cggccgcagt tctatctgcg
    14221 cacgaccgat gtgaccggtg ccatctcgat gcctccgggc gtggagatga tcatgccggg
    14281 cgacaacgtc aacatcgacg tcgagctgat cacgccgatc gcgatcgagc agggtcagcg
    14341 cttcgccatc cgcgagggcg ggcataccgt cggcgcgggc gtcatcacgg cgctggtcgg
    14401 ctgaatcgac gatggccaaa cagcgcatcc gcattcggct caaggcgtac gaccacaagc
    14461 tgctcgacca gtcggccgag cagatcgtgg agacggcgca gcggaccggt gccgccattg
    14521 cggggccggt tccgctgccg acgcggatcg agaagtacac ggtcattcgc ggcccgttcg
    14581 tccacaagga cggccgggag cagttcgaga tccggacgca caagcggctg atcgacatca
    14641 tcgacccgtc gaccaagacg gtggatgcgc tgatgcggct gagcctggca gcgggcgtcg
    14701 acatcgaaat caagatgtga ttggggaacg agagaagcag tgccggcagg attgatcggg
    14761 cgcaagctcg ggatgacgcg gatcttcggt gacgacggga gcgtcgtgcc ggtgagcgtc
    14821 atcgaggcga cgcccaacaa cgtcacgcgg ctccgcacga cggagcggga cggctatgcc
    14881 gcgctgcagg tcggagccgg ctcggccaag cgacagagca agccggtcgc cggccagttc
    14941 aaggagctcg ccaaggagct gcagcggccg cgtcacgttc gcgagttccg agtcgagtcg
    15001 accgagggct acgagctcgg ccagcaggtc gacgtcaacc tcttcgcgcc cggcgacctg
    15061 gtggacgtga ccggtgtcag caagggccat ggcttcaccg gcacgatcgc caggcaccac
    15121 ttcaagcgcg gccccaagac ccacggctcg accaacattc gccagccggg gtcgatcggg
    15181 gccggcacca cgccaggccg cgtcttccgc gggacccgga tgggtggcca catgggtgac
    15241 gaccgggtga ccgtcaagaa gatgcaggtc gtcaaggtcg accccgagcg aaacctgatc
    15301 ctggtcaagg gcgccgtgcc gggcgcccgc aacgccatcc tgctcgtgca gaaggcgaag
    15361 tgacgatgga gcaggcaccg atcctcaccg tcagcggctc ggagtcgggc aaggtggacc
    15421 ttgtcccctc gctcttcgcc gcgcccgtga acgaggccct catccatcag gccatcgtcc
    15481 gccagctggc gggccagcgt ctcgggaacg ccgatacgct gacgcgcggt caggtccgcg
    15541 gcggcggcaa gaagccatgg cgccagaagg gaaccggccg cgctcggcag gggacccgga
    15601 gcgccccgca gtggaagggt ggcggggtcg tcttcggccc gcacccgcgc ggctacgagc
    15661 agcgaatgcc ggccaagatg cgccgcgccg cactgcaggg cgtcctgagc ggcaaggccg
    15721 acgacggtgc tctccgggtc gtcgagggat tcggcctcga gacgatgggg accaaggcct
    15781 ttgccgagcg actcgtcgcc tggaagaccg agggcaaggt gcttgtcgtc ctgccagcgc
    15841 gagatccggt cgtcgagcgc tcctgccgca acctgcgcga ggtgcgcgtt ctccttgccg
    15901 acagcctgaa cgtggtcgac ctgctggagg ccgacacgat cgtcttcacc gccgatgcgc
    15961 tggcacgcgc gcaggaggtg tacggatgac cgccgtcagc gttcacgaca tcctcattcg
    16021 ccccgtcatc agcgagaaga gcgtcgccca gaccgagcgc aacaactaca ccttcgcggt
    16081 cggccgcgac gcgaacaagt tccagatcaa ggacgccgtg gaggcagagt tcaaggtgac
    16141 cgtcctgggc gtacgcgtgc tctgggtcaa gccaaagcag aagcggcgtg ggcgccagac
    16201 gatgggcacc gtgcccggct ggcgcaaggc ggtcgtcacc atcgccgccg gacagaagat
    16261 cgaactcttc gaggcggtgt aacgagatgc cacttcgcaa ctaccgaccc accagccccg
    16321 gactgcgcca gatgacgcgg tcgaccttcg acgagatcac gaccgatgag ccacacaagc
    16381 cgctcaccga gcgcctcgtg cgcaaggctg gccggaacag ccagggcagg ctgaccgtcc
    16441 gccaccaggg cgccgggcac aagcggctgt atcgggtgat cgactggaag cgcgacaagt
    16501 tcggggtccc ggcaaccatc gcaacggtgg agtacgaccc gaaccgctcg gcgcgcatcg
    16561 gcctcctgca ctacgccgat ggcgacaagc gctacatgct gctgccgcac ggcctcgggg
    16621 tcggcgacgt ggtcgtctcc ggccgggacg ctgaagcccg cgtggggaat gccctcccgc
    16681 tggctcgcat gcccctgggc acccagatcc acaacgtgga gatggtcgcc ggccgcggcg
    16741 gtcagatcgt gcgcagcgcg ggaagcagcg cccagctgct cgccaaggag ggcgagatgg
    16801 ccaccatccg gctcccctcc ggcgaggtgc ggcgagtccg ggccgactgc atggccacgg
    16861 tcggccaggt gagcaacctc gatcacgaga accagaacat cggcaaggca ggacgcagcc
    16921 gccacatggg acggcggcca gaggttcgcg gcgtcgtgat gaacccgcgc gaccatccgc
    16981 acggcggtgg ggagggcaag tcgccgacgg ggatgccgcc caagacaccg tggggcaagc
    17041 ccgccatggg gctgcgcacc cgcaagaaca agacgaccgg caagatgatc gtccgtcgcc
    17101 ggtatggacg aggttaggag agtccgatga gccgttccgt caagaaggga cccttcgtcg
    17161 atgcccgcct cctcggccgc atcgacgaga tgaacaagca caacgagcac aaggtgctga
    17221 agacctggtc gcgaccgagc gtgatcttcc cacagatgat cgggcacacg atcgcggtgc
    17281 acgatggcaa gcgccacgtc ccggtgttcg tcaccgagaa catggtcggg catcggctcg
    17341 gcgagttcgc gcccacccgt cactaccgag gccatggcaa ggccacggat cgctctacgg
    17401 cactgaagta ggaatgcagg agatgcgtgt catcgccacc gccaagtaca tccgcatcag
    17461 cccccgcaag gtgcgcctgg tgaccgatct gatcaccggc aagccggtcg aggaggctgc
    17521 cgccatcctg cgcttcctgc cgaatgccgc cgctcgcgac gtcgccaagg tgctcaagag
    17581 cgcgacggcc aatgccgaga acaacttcaa cctgtcggcc gatgagctcc acgtgctgag
    17641 cgccaccgcc aacgagggac cgaccctcaa gcggttccgc ccccgggccc aggggcggac
    17701 cttcagcatc ctgaagcgaa ccagccacat cacggtcgcc gtggccgatc gagaggagtc
    17761 ctgatgggac acaaggttca tccctacggc ttccggatcg ggatcaacaa gccctggctg
    17821 gcgaagtggt acgccgacaa ggactacgcg accctgctcc aggaggacat gaagatccgc
    17881 gcgctggtca gcaagcagct ggccaacgcc agtgtcagcc aggtcgagat cgagcgtggc
    17941 atcaaccacg tcaccgtgac ggtgcactcc gccaagccgg gcatcgtcat cggcaagggc
    18001 ggacagaacg tcgagctgct gcgcacctcg atcggcaagc tgaccgacaa gaaggtcaag
    18061 ctcgagatca aggagatcct gcagccggag ctcgacgcgg tcctggtcgc gcagaacgtt
    18121 gcgggccagc ttgagcgccg gatcgccttc cgcaaggcgg tcaagcagac cgtgcagcgc
    18181 accatcaagg ccggggccaa gggcgtcaag atccaggtct ccggtcgctt gggcggggcc
    18241 gagatgagcc gcaccgagtg ggacaaggag ggccgcatcc cgctggggac gctgcgcgcg
    18301 gacatcagct acggggtcgt ccacgcccgg acctcgtacg gccgcatcgg cgtcaaggtg
    18361 tgggtctacc gcggcgacca gctcggggag cgacccggct cggcacgcac ggagccgcgt
    18421 gagccgcgac agcgtccaac cacccgaacc cgcgtcaagg cggccacccc cgcagtcgcg
    18481 gcgaccgccg gtgcggattc gccgcccgcc gctgccggcg aggaggcctg agccatgttg
    18541 atgccgaagc gagtcacgca ccgcaaggtc cagcgtggcc gccgcgcggg catggcaaag
    18601 ggagggacca ccatcagctt ctgcgagtac gggctgatgg ccgaggaggt gtgctggctc
    18661 accagccgtc agatcgaggc ggcgcggcgt gcgatgactc accacatcaa gcgcggcgga
    18721 cggatctgga tccgtgtctt cccggacaag ccagtcacca agaagccggc cgaggttcgg
    18781 atgggatcag gcaagggcgc ccccgaccac tgggtcgcgg tcgtcaagcc cggccgagtc
    18841 atgttcgaga tgtccggcgt gccggaggac atcgcccgcg aggcgatgcg actggccagc
    18901 cataagctgc cgattcagac acggttcgtc gccaaggagg gcgcgtgatc atggatatca
    18961 acgagatccg gggcctgtcg gacctcgacc tggagagtgc gctggtcgac gccaagcagg
    19021 agctgtggaa gatccgcttc gacctggcca cgcgccagga gaagaactac agccggctgc
    19081 ccgcgacccg caagcggatc gcccgcatcc tgaccgtgat gactgaacga cagcatgcgg
    19141 ccgacgacgc cgcggccgcc gtcgggaagg ccagctgatg gcaggcaagc gccgcaccaa
    19201 ggttggccgc gtcatctcgg acaagatgga caagacggtc gtcgtgagcg tcgagcggct
    19261 tcgccgccat ccgatctaca agcgcgtggt ccggctgtcc agcaagttca aggcgcacga
    19321 cgaggagaac tcggcacacg tcggcgacac ggtgctgatc gaggagagca agccgctctc
    19381 tcgcgagaag cgctggcgcg tcgtcgaggt ggtcgctcgc ggatcaggta aggagatggt
    19441 ggacaccgca tgatccggca gtacacacgg ctgcgagtcg ccgacaacac gggcgcgcgt
    19501 gagctgatgt gcatcaccgt cattggtcgc tcgagcagcg ataccgccga ggtcggcgat
    19561 gtcatcgttg ccagcgtcaa gcaggcgctg cccaacaccg gcgtgaagaa gggcgacgtc
    19621 gtgcgtgccg tgatcgtgcg caccgccaag gagtacggcc gccccgacgg cagccacatt
    19681 cgcttcgacg agaacgcggc ggtcctgatc acaccccagg gcagcccgcg cgggacacgg
    19741 atcttcgggc ccgtggcccg ggagctccgc gagcggaact acatgaagat cgtgtcgctg
    19801 gcaccggagg tgctgtgaga tgaacattcg caagggggac accgtcctcg tcctggccgg
    19861 caaggatcgc gggaagcggg gcgtggtcga gcgcatcgag cgcaccaagc gcggccttgg
    19921 cgtcgtggtc cccggcctca acatggccaa gcggcatcag cgaccgcgga cgcggaccca
    19981 gcaggccggg atcctcgacc tgcccgtgcc gcttcacctc agcaacgtgc aggtcgtctg
    20041 cccgcgctgc gaccgcccga cccgggtctc acacgagctg ctcgagggcg gctcgaagca
    20101 cgttcgcgtc tgcaagcact gcggggagca gatggaggtg gcccagtgag cgacgaagaa
    20161 gctaccccga aggccgctcc caaggcgcct cgccgcagtc ctcgccgccc cgcggcggca
    20221 acgccggccg cccagccgac agctcagccg gccgaatccg gcccagcggc ggagaccggt
    20281 gggcttcgtc gccgctacct cgacgaggtg gcgccggcca tgcgccgtga gttcggctat
    20341 gccaacctga tgcagatccc gcgcctcgag aaggtggtcg tcaacatcgg cctgggcgag
    20401 gcgatccaga acgccaaggc gctcgatgcg gcggtggggg acctgaccca gatcacgggt
    20461 cagaagccga tcgtcacgcg cgccaagcgc tccatcgcgc agttccggct gcgaaccggc
    20521 atgccgatcg gggccaaggt gacgcttcgc ggacagcgga tgtacgactt cctggagcgg
    20581 accatgcgac tggcgctgcc gcgcatccgg gacttccgcg ggatcagccg caactcgttc
    20641 gatggtcgcg gcaatttcag cctggggctg cgggagcagc tcgtctatcc ggagatcgac
    20701 tacgacaaga tcgatcgcct gcgggggatg gagatcagcg tcgtcaccac cgccaagacc
    20761 gacgaggagg ggctgaagct cctccagctg ctcggtatgc cgttccaggc cagctagcga
    20821 ggattcgagt ggccaagaag tcgatgatcg tgaagtcgca gcggaagccg cgcttcgcgg
    20881 tccagcacaa gagtcgctgc gcagtctgtg gacgtccgcg cggctatatg cggcgcttcg
    20941 cgctgtgccg catctgcttc cgggagcgag ccctcgaggg tcagctgccc ggcgtcacga
    21001 agtcgagctg gtagctcgaa gagccaatcg gccgccacat cgcggcccca tcagcggcag
    21061 gcgccgaagc cgaagggcac ggggcgaacc gccgccgagg agagacacaa gccaacatga
    21121 atgtgac
//
LOCUS       DATCQR010000121         3454 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_146544, whole genome shotgun sequence.
ACCESSION   DATCQR010000121 DATCQR010000000
VERSION     DATCQR010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3454
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146544"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..964
                     /locus_tag="VIN32_04095"
     CDS             <1..964
                     /locus_tag="VIN32_04095"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921794.1"
                     /translation="GSGIEWLDRLAAEHDNVRAALRWSVDSGQREIGLRIAAALWRFW
                     QQRGHLIEGRAWFDRLIPDPAEADSVDAGVLAAAHTAAGGVVYWQNDQDQAEAHYLAA
                     YELDRAHDRLDRIGDDLYNLAFVAIVRKEIQTGRERFEESGKAFAAAGQSDRLADSTA
                     ALGALEMRAGNLDKARDLMEEGRRLHAEQGNHARATDNTMVLSNIYLQRGEVETAREY
                     LRKAVAAIREMKDVARFPLALDLGAAQALSDGRPADALRLLAAAALRRAQVGGGTPNF
                     VVNNPEMIVEARAAMEQQGAGDEADKGWAEGETLDDDALAALIG"
     gene            1030..1290
                     /locus_tag="VIN32_04100"
     CDS             1030..1290
                     /locus_tag="VIN32_04100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921795.1"
                     /translation="MPTREKRTYNLTPATVRAVRELADDYHAAPTQDAVVELAVSELA
                     RRLRDEQESAAWEAAAADPAFRAETQGIENAYRDADRETWPA"
     gene            1302..1664
                     /locus_tag="VIN32_04105"
     CDS             1302..1664
                     /locus_tag="VIN32_04105"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEY8921796.1"
                     /translation="MRWGMVVVALDPVVGHEQAGTRRALVVSYEPFHRAGLMTICPVT
                     AARAEARYPGDVAIPAGEAGQTKPGVIICSQVRTISAQRMVGAPVGFLTDADLQRRVR
                     QALAHHLGLDIPATVDRV"
     gene            1691..1996
                     /locus_tag="VIN32_04110"
     CDS             1691..1996
                     /locus_tag="VIN32_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012947003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HEY8921797.1"
                     /translation="MKAGKTRPERVGVRELRQNLSVYLRHVREEGRAYEVTERGEPVA
                     HLTPMPDRRLSTYERMLAEGRVALAKHPWDELPEPLPPLPGKPLSEILREMRDEETW"
     gene            1993..2391
                     /locus_tag="VIN32_04115"
     CDS             1993..2391
                     /locus_tag="VIN32_04115"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEY8921798.1"
                     /translation="MTKRVYLDASAAVKLVVSEPESASVRMFLAGEVSRVSSRVLAVE
                     LIRAVIRGSPASLDQARSLLGVIEFIELDEEVAERAAGLEPAALRSLDAIHLATALAL
                     GEDLDAFVTYDARQGDAARTLGLIVEAPSP"
     gene            complement(2403..2753)
                     /locus_tag="VIN32_04120"
     CDS             complement(2403..2753)
                     /locus_tag="VIN32_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005516798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UBP-type zinc finger domain-containing protein"
                     /protein_id="HEY8921799.1"
                     /translation="MADQPPIDPTIPPSGTGCVECLATEDGWWFHLRRCVHCGHIGCC
                     DQSPSQHGSKHAKAEDHPIIRSFEPEEDWFYDYTQDEYAEGPELAPPLHHPLSQPTPG
                     PAGRVPRDWESRLN"
     gene            complement(2756..>3454)
                     /locus_tag="VIN32_04125"
     CDS             complement(2756..>3454)
                     /locus_tag="VIN32_04125"
                     /inference="COORDINATES: protein motif:HMM:NF039315.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5996 family protein"
                     /protein_id="HEY8921800.1"
                     /translation="RTFEMTLAPMSVATFYREFMAGLRGMGIDVRIWTTPVEVADPIP
                     FELDEQHASYEPVHANLFWRALLQADRVMKEFQTGFVGKVSPVHLFWGGLDLATSRYS
                     GRPAPLHPGGIPNTADWVNQEAYSREEFASGWWASTDPPGPAFYSYTYPEPDGLTSAA
                     IRPAEAFFDSRLDEFALSWEGARSTRDPDAAVLEFFQSTYEAGADLAGWDRALLEPSE
                     IPARPPKRPWSIMR"
BASE COUNT          558 a         1084 c         1294 g          518 t
ORIGIN      
        1 gggcagcggg atcgagtggc tcgaccggct ggcggcggag catgacaacg tgcgcgcggc
       61 gctccgctgg tcggtggaca gcggtcaacg ggagatcggc ctgcggatcg cggccgccct
      121 atggcgcttc tggcagcagc ggggccatct gatcgagggt cgcgcctggt tcgatcggct
      181 catccctgac cctgcggagg ccgactcggt ggacgcaggc gtgctcgctg cggcgcacac
      241 cgccgctggg ggagtcgtct actggcagaa cgaccaggat caggccgagg cgcactacct
      301 cgccgcctac gagctcgacc gcgcccacga tcggctcgac cgcatcggtg atgacctgta
      361 caacctcgcc tttgtggcga tcgtgcgcaa ggagatccag accgggcggg aacgattcga
      421 ggagagcggg aaggcgtttg ccgcggcggg tcagtcggac cgcctcgccg actcgaccgc
      481 tgcgctaggc gccctcgaga tgcgagccgg gaacctcgac aaggcgcgcg acctgatgga
      541 ggaggggcgc cgcctccacg ctgagcaggg caaccacgcc cgggccacgg acaacacgat
      601 ggtgctgagc aacatctacc ttcagcgggg tgaggtcgag acggcgcgcg agtacttgcg
      661 gaaggcggtg gcggcgatca gggagatgaa agacgtcgca cgatttcccc tcgccctgga
      721 tcttggggcg gctcaggccc tcagcgacgg gcgtccggcg gacgcgcttc ggctccttgc
      781 ggcggctgcg ctgcggcgcg cccaggtcgg cggcggaacg cccaacttcg tcgtgaataa
      841 tccggagatg attgtcgagg cgcgggccgc gatggagcag cagggcgccg gggacgaggc
      901 cgacaagggc tgggcagagg gggagaccct ggacgacgac gcgctggccg ccctcatcgg
      961 ctgatgagag ccccggtgct tggatgggtg ctgattgaca cgtcgatccg gacccatcac
     1021 actgcgccaa tgccaaccag ggagaagcgg acctacaacc tcacccccgc gaccgtccgc
     1081 gcggtgcgcg agttggccga tgactaccac gcggccccca cccaggacgc ggtcgtggag
     1141 ctggccgtct ccgagctggc acggcggctg cgtgacgagc aggagtcggc cgcatgggag
     1201 gccgcagcgg cggatcccgc gttccgcgcg gagacgcagg ggatcgaaaa cgcctatcgc
     1261 gacgccgatc gcgagacgtg gccggcctga ggatcgggcc attgcgatgg gggatggtgg
     1321 tggtggcgct tgatccggtg gttggtcacg agcaggccgg cacgcgacgc gcattggtgg
     1381 tcagctacga gccgtttcat cgggccgggc tcatgacgat ctgtcccgtc acagcagctc
     1441 gcgcggaggc gcgctacccg ggggacgtcg cgattccggc gggcgaggcc gggcagacga
     1501 agcctggcgt gatcatctgc agccaggtgc gcaccatctc cgcgcaacgg atggtcggcg
     1561 ccccagttgg cttcctgacc gatgcggacc tgcaacgacg agtccgtcag gctctggccc
     1621 atcacttggg cctcgacatt ccggcgaccg tcgaccgggt ctagagttcg atgtgctaca
     1681 gttgtgcgtc atgaaagccg gaaagacgcg gccggagcgc gtaggggtgc gcgagctgcg
     1741 ccagaacctg tcggtctacc tcaggcacgt gcgtgaagag ggcagggcgt atgaggtgac
     1801 ggagcgcggg gagccggtgg cgcacctgac gcccatgccg gaccggcggc tgtcgacgta
     1861 cgagcgcatg ctggcggagg gccgcgtcgc gctggcgaag catccctggg acgagctgcc
     1921 ggagccattg ccgccactgc caggaaagcc gctctccgaa atcctgcgcg agatgcgcga
     1981 cgaagagacc tggtgacgaa gcgcgtctac ctcgacgcgt ccgcggccgt gaagttggtc
     2041 gtcagcgagc cggagtctgc atcggtcagg atgtttctgg cgggtgaggt gtcgcgggtc
     2101 agcagtcggg tgctggcggt agagctgatc agggcggtca tccgcggcag cccagcctcg
     2161 ctcgatcagg cacggtcgct cttgggggtc attgagttca tcgagctgga tgaggaggtc
     2221 gcggagcgcg ccgcaggact cgagccggct gcgttgcgat ccctggatgc catccacctt
     2281 gccacggcgt tggcgctcgg cgaggacctt gacgccttcg tcacgtatga cgcccggcag
     2341 ggggacgccg ctcgcacgct ggggctcatc gtcgaggcgc cgtcgcccta ggttctgccc
     2401 agctagttca gacggctctc ccagtcacgg gggacgcggc cggccggacc gggcgtgggc
     2461 tggctcaagg ggtgatgcag cgggggagcc agctcagggc cttcggcgta ctcgtcctgc
     2521 gtgtagtcgt agaaccagtc ctcctccggc tcgaagctgc ggatgatcgg gtggtcctcc
     2581 gccttcgcat gcttgctgcc gtgctgcgag ggggattggt cgcaacagcc gatatggccg
     2641 caatgcacgc atcggcgcag gtggaaccac cagccatcct cggtcgccag gcactcgacg
     2701 cagccggtgc cgctgggcgg gatggtgggg tcgatcgggg gttggtcagc catgctcagc
     2761 gcatgatgct ccatggccgc ttcggaggtc gggccgggat ctcagagggc tccaggaggg
     2821 cccgatccca gccggcaaga tcggcccccg cctcgtaggt ggactggaag aactcgagga
     2881 ccgcggcgtc cgggtcgcgg gtgctgcgag ccccctccca actcagggcg aactcgtcga
     2941 ggcgggagtc gaagaacgcc tcggccgggc ggatggcggc ggacgtcaga ccatcgggct
     3001 ccgggtaggt gtaggagtag aaggcggggc cgggaggatc ggtggaggcc caccagccgg
     3061 aggcgaactc ctcgcgcgaa tacgcctcct gattcaccca gtccgcggtg ttcgggatcc
     3121 cgcccgggtg aagcggcgcg gggcggccgg aatagcgcga ggtcgcgagg tccaggccgc
     3181 cccagaacag gtgcaccgga ctgactttgc cgacgaaccc agtctggaac tccttcatca
     3241 ctctgtcggc ctggaggagg gcgcgccaga agaggttcgc atgcaccggc tcataggagg
     3301 cgtgctgctc gtccagctcg aagggaatgg ggtcggccac ctcgaccggc gtggtccaga
     3361 tccggacgtc gatccccatc ccgcgcagcc cggccatgaa ctcgcggtag aaggtggcga
     3421 cggacatggg ggcgagggtc atctcgaagg tgcg
//
LOCUS       DATCQR010000122         2996 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_69399, whole genome shotgun sequence.
ACCESSION   DATCQR010000122 DATCQR010000000
VERSION     DATCQR010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2996
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69399"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..907
                     /locus_tag="VIN32_04130"
     CDS             <1..907
                     /locus_tag="VIN32_04130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8921801.1"
                     /translation="VKRVLERAGFECVTVDNGEVAHESAVDWRPDIILLDLMLGSTTG
                     DQILADIRKDFRTRLIPVVFLTVRSSLKDKVEHLLAGADDYVTKPFIAEELVARLRAV
                     ISRSTNTRDLNPLTGMSGNTDILREINLRLKANERFAVLYPDIDTFKSYNDHYGFLRG
                     DDVIKTLGTIILEVLEAHYSPHHFGGHVGGDDFVILTEPGLAESVAAEITKRFDEAIP
                     DLYDEVDRVRGWIEYEDRSGSALKTGLVSVSIGIVVAEPGSYESAAALASRAAEVKGV
                     AKRMPGSRWVVDRRRPPEARGGRTR"
     gene            984..>2996
                     /locus_tag="VIN32_04135"
     CDS             984..>2996
                     /locus_tag="VIN32_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012623412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HEY8921802.1"
                     /translation="MSDFEPTTSTTPQVLTDDAPSAVIAARTPEPGAAKVARRQVAVH
                     RLDGGLERGESDARSLSPDGFPIYTPPDLDRARWIPVRDIKYVVFGSLDDPDLEADPG
                     EKSQARKAILRFRDGEWLAAYMDSGQPPDGAGLAIKIRLTERQRVIPAIAANPALLEM
                     QFVDLWATTTDSAQPHRRRSDIVEAAARQGRDLQTLANDFRDRLALIRDVGLTTGDTL
                     AFSRAVRTHLDRFLAEDGINLNSVEKSALADIILRAAVGYGPLDSLLHDRSVSEIMVN
                     GPDQVFIERRGVLSKADIRFEDENQLLETIRRMVATTGRHIDGLNPMVDARLPDGSRV
                     NAIIRPAALHGAALTIRKFKDAVLGIDDLTREGSLSPAMAEFLKAAVLGRMNILVSGG
                     TGSGKTTTLNVIARFIPHNQRVITIEDAAELQIDHPHVVALEHRPANVEGKGELTIRQ
                     LLRNSLRMRPDRILVGEVRGSEALDMLQAMNTGHDGSMSTIHSNSARDALSRLETMVM
                     MASIDIPFEAVRAQIASAVNLIVHQARMPDGRRKVAQIAEVVGYDSNGAILRDIFLLG
                     MGADLRLEYNATGYIPTSLDKAAFYGVQVDQELFDPVKSRFVPAGSDSMMPVVKDPLT
                     SGQRGGGGENIVRQVVVVPFSSDRPGDRQSQQQQHTVQTTASGPTTT"
BASE COUNT          552 a         1061 c          916 g          467 t
ORIGIN      
        1 ggtcaagcgc gtgctcgagc gtgccggttt cgagtgcgtg accgtcgaca acggagaggt
       61 cgcgcatgag tcggcggtcg attggcgccc ggacatcatc ctgcttgacc tcatgctcgg
      121 gtccacgacg ggcgaccaga tccttgccga cattcgtaag gatttccgga cgcgcttgat
      181 ccccgtcgtg ttcctcacgg tccggagctc cctgaaggac aaggtggagc acctacttgc
      241 cggagcggac gactatgtga cgaagccgtt catcgcagag gagctcgttg cgcgattgcg
      301 agctgtcatc agccgctcca cgaacacgcg cgacctcaac ccgctgaccg ggatgagcgg
      361 aaacaccgac atccttcgcg agatcaacct gcgcctcaag gccaacgagc ggttcgcggt
      421 cctctatccc gacatcgaca ccttcaagag ctacaacgac cactacggct tccttcgcgg
      481 agacgacgtg atcaagacgc tcggcacgat catcctcgag gtgctcgagg cgcattactc
      541 gccacatcac ttcggcggcc atgtcggcgg cgacgatttc gtgatcctga ccgagccagg
      601 cctggccgag tcggtcgcag ccgagatcac caagcgcttc gacgaggcga tccccgacct
      661 gtacgacgag gtcgaccggg tacgcggctg gatcgagtac gaggatcgca gcggctccgc
      721 gctcaagact ggcctcgtct ctgtgtcgat cggcatcgtc gtcgctgagc ccggcagcta
      781 cgagagcgcc gccgccctcg cctcccgcgc agccgaggtc aagggcgtgg cgaaacgaat
      841 gcccggatcg aggtgggtcg tcgaccgccg ccgcccgcca gaggctcgcg gaggccgcac
      901 acgttaatga caccgttaat gaggagaagg accgggttcc cgatgctagc atcgaccaat
      961 tccacccacg cgggtgattg ccaatgagtg acttcgaacc aaccacatcc acgactccgc
     1021 aagttctgac cgatgacgcg ccgtctgcgg tgatcgctgc acgcacgccc gagccaggcg
     1081 ccgccaaggt cgcccgccgc caggtcgcag tgcatcgtct cgatggcggc ctcgagcgcg
     1141 gcgaatccga tgcacgatcc ctgagcccgg acggcttccc gatctacaca ccaccagacc
     1201 tggatcgcgc gcgctggatt ccggtgcggg acatcaagta cgtggtcttc ggctcacttg
     1261 acgaccccga cctcgaggcc gacccgggcg agaagagcca ggcgcgcaag gcgatcctgc
     1321 gcttccgtga cggcgagtgg cttgccgcct acatggactc ggggcagccg ccggacggcg
     1381 ccggcctcgc catcaagatc cgcttgaccg agcgccagcg cgtcattccc gccatcgccg
     1441 ccaaccccgc gctgctggag atgcagttcg tcgacctgtg ggccaccacg accgactcgg
     1501 cgcagcccca ccgccggcga tccgacatcg ttgaggcggc ggcgcggcag ggccgcgacc
     1561 tgcagacgct cgccaacgac ttccgcgacc ggctcgcgct gatccgcgac gtcggcctta
     1621 cgaccggcga tacgctcgcg ttctcgcgcg ctgtgcgcac gcacctcgac cggttcctgg
     1681 ccgaggatgg catcaacctc aactcggtcg agaagtcggc cctcgccgac atcatccttc
     1741 gcgccgctgt cggctatgga cctctcgaca gcctgcttca cgaccgctcg gtcagcgaga
     1801 tcatggtcaa cggaccggac caggtgttca tcgagcggcg gggcgttctg agcaaagccg
     1861 acatccgctt cgaggacgag aaccagctgc tggagacgat tcgccgcatg gtcgcgacca
     1921 ccggccgcca catcgacggt ctgaacccaa tggtcgacgc ccgcctgccc gacggcagcc
     1981 gtgtcaacgc gatcatccgg cccgccgccc tccacggcgc cgctctcacg atcagaaagt
     2041 tcaaggacgc cgtcctcggt atcgacgacc tgacccgcga gggcagcttg agccccgcga
     2101 tggcggagtt cctcaaggcc gcggtgctcg gccgcatgaa catcctggtc tccggcggaa
     2161 ccggatccgg caagaccacg accctcaacg tcatcgcgcg cttcatcccg cacaaccagc
     2221 gcgtgatcac catcgaggac gccgccgagc tgcagatcga ccacccgcac gtcgtcgccc
     2281 tcgagcaccg accggccaac gtcgaaggca agggcgagct gacgatccgc cagctgctac
     2341 gaaacagcct ccgaatgagg cctgaccgca tcctcgtcgg tgaggtgcgc ggctcggaag
     2401 ccctcgacat gctccaggcc atgaacaccg gccacgacgg cagcatgtcg acgatccact
     2461 ccaactcggc gcgcgacgcg ctgtcccgac tcgagacgat ggtgatgatg gcgtcgatcg
     2521 acattccctt cgaggctgtg cgcgcgcaga tcgcctccgc cgtcaacctc atcgtgcacc
     2581 aggcccgcat gccggatgga cgccgcaagg tcgcgcagat tgccgaggtc gtcggctacg
     2641 actccaatgg tgcaatcctt cgcgacatct ttctcctcgg catgggtgcg gatctacggc
     2701 tcgaatacaa cgcgaccgga tacatcccga cctcgctcga caaagcagcc ttttatggag
     2761 tccaggtcga ccaggaactc ttcgaccctg tgaagtcgcg cttcgtccca gcgggctcgg
     2821 acagcatgat gcccgtcgtc aaggatccgc tgacgagcgg gcagcgcggc ggcggcggcg
     2881 agaacatcgt ccgccaggtc gtggtggtgc cgttcagctc ggaccgccca ggcgatcgac
     2941 agtcgcagca gcagcagcac acggtccaga ccacggcctc ggggccgacc acgacg
//
LOCUS       DATCQR010000123         7686 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_53470, whole genome shotgun sequence.
ACCESSION   DATCQR010000123 DATCQR010000000
VERSION     DATCQR010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 7686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7686
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53470"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /locus_tag="VIN32_04140"
     CDS             complement(<1..401)
                     /locus_tag="VIN32_04140"
                     /inference="COORDINATES: protein motif:HMM:NF023325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3417 domain-containing protein"
                     /protein_id="HEY8921803.1"
                     /translation="MTSAVRVPSQPGYDFGLPPALEGLGELAYNLWWSWTPRAGALFA
                     RIDSAAWSRYRSPIPVLAAVEPGRWTELLADEDFMVDASRVLDEFERYLANGSDSWYR
                     AATDSDPSATLPGPIAYFCAEYGIHESMQIYS"
     gene            510..2510
                     /locus_tag="VIN32_04145"
     CDS             510..2510
                     /locus_tag="VIN32_04145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921804.1"
                     /translation="MDGYSVQEAASVLGVAEGRVWELLARGVLLGTPEGDSMRVFLKA
                     QAGPIASAGTPDEPPRTNGNGGSHGPAGEASAFRELLTEFRNLTERYGQALLALGEAR
                     GEVAGLRSRVDQLEARLDLRLPPPVDAAPVAWDSLSVPATTPEKARAMPPSPAPRAPV
                     RRKLKPKARQRKARSPREALAGSPREAVAGFAEALARAQDPNAADLGEARVETEPLSE
                     ANIEPASATAVAPDEPMQEAADTPELVAREVGSDEIREDELAAEEAAPAPVATAQPTA
                     ELVVEEASPDEPTAQVEAPTYSAVVVEPDWFADGDFAWLDAAEMEGSAEASSATATPD
                     KAEQEPEQAAPEAEAPAAESEPEVQAAESEPEVQAAEPEPEVQAAEPEPEQQQLQEAQ
                     VAPEPPPQPETGFGPWQPPEPKPVESQDPEIAAEPDLEREPPSQFEAEAEFESWQPPE
                     PASPEAPAEPEVADAPEPPTASAAVAVEEVEEEVMWLGRESDPSPVIHDEPSPDYRAE
                     AEEWPRIDAAPTMEAVRPPLAMTEDELARLALDEGWDDTEVAAIRAMITPPAMSAVDL
                     PGAAELHEAMAALEAVPVQTDPPYDASRQWAKPAPDDEEPARHDDWAFEAEPAPPPPA
                     PTRPPSDVTSALRRALEDPGWLRRRQGPAAEAYRRLRRLFPG"
     gene            2591..3022
                     /locus_tag="VIN32_04150"
     CDS             2591..3022
                     /locus_tag="VIN32_04150"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEY8921805.1"
                     /translation="MALIGLVVVLGSPGLRDISPHIASGWVGLLVGMAAIVIGAWLVI
                     RAVADLGRSLSPMPRPRPDGRLIDTGIYARLRHPIYAGVIFASFGWSVLTGSLGALVA
                     ALLLAIFLDAKARREEIWLLERYDAYADYRQRSKRFLPGIY"
     gene            3078..3548
                     /locus_tag="VIN32_04155"
     CDS             3078..3548
                     /locus_tag="VIN32_04155"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HEY8921806.1"
                     /translation="MTRTIHDTRTVAADLLDELGPIIAGERAAFALRCHHRSISMTHL
                     LLMTLLETHGPLPMSRVAELLGCGLPAATGIVSRMQERGLVERQHDSEDRRVVTLSLS
                     DEGAVEIRELHLSRRERMATALTHLSEPEREQLLASVRSLRAAFNRANSQGDNA"
     gene            3545..5209
                     /locus_tag="VIN32_04160"
     CDS             3545..5209
                     /locus_tag="VIN32_04160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HEY8921807.1"
                     /translation="MTAAAEPMDAPVHVDPRARTEILIAILLAMFLSALDQTIVGTAL
                     PTIMTQLHGNDYYTWVVTIYLLTATISGPIYAKLSDLFGRRPMVMIGVTLFLIGSALS
                     GLSQEAWQLIAARGLQGLGAGAIFPIALAVIGDLFTPAERGKYQGLFGAVFGISALIG
                     PALGGFLTDNVGWHWIFFVNLPIGAVALFIIWRLLPAIKHPERARSIDYLGSVVFTAA
                     LVPILIGLTNKATMDWTDPQVGGLLLVGFVLAAVFVWVESRASEPILPLSLFRNRSIA
                     VSLIAVFLAGFGFFSAIIFVPRWFQVVLGSSATESGYQILPLLAGLIISSVISGQVIS
                     RTGRYRWIIVISMAFLAVGLLLMTQLRAETSLPEMWFWMVVAGIGIGPTLAAFTIVVQ
                     NAAPFAQLGAATGALTFFRQVGGTVGLAIGGTLFGSALVTEIPQQMAKAGVPSDIAAR
                     FATSGVATDVSSVGDLGARILKAVPEQVRPLVDPYIPKIVFAFHEAFSLAIASALWVS
                     LAAAVIALVVVTIALPELPLRTHHGPARAGARPEDGSEPAPIAALD"
     gene            complement(5217..>7686)
                     /locus_tag="VIN32_04165"
     CDS             complement(5217..>7686)
                     /locus_tag="VIN32_04165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921808.1"
                     /translation="TYAPMRSLLLQLLDLGDESAADEIRTQARRWLRDVGDEDADRTA
                     ERLIATIGSADAEVVDRGDIFAAWRHTVELAAADNPLVLVIEDLHWSSESLLDLIEYV
                     LQPRTDAPLLMLVLTRPELLDRRPSWGGGRRNHVSLALDPLEPNEVSRLVELILEGPA
                     PELVRLVVERAEGNPFYVGELVRSIRERAAALDDPVALQAAIATLPDTVQATVLARLD
                     LLDGPARRLLQLGSVHGRSFRLAGVQALEPEAKLDLAGAAERLVERDLVRPSGGDRFS
                     YRHILIREVAYSTLPRSERARLHAAAGHWLAATSAGREDELAELIAFHLREAAALTAS
                     TGMRDVAIDAAAVEWLRRAGEVAFASMANVEASRHLLAAIELADPTQLPELYGRLGDT
                     YLAGDQSVEAYATALRLGRDAGRPPDFLLRVLGEMLFVMSRWYASVAGQPAEDDFARL
                     RDEARALFEVAKDRRAKAVFLISEGFVAFWLRNSGVRQPSAEEIAHGMASAQAGMQLA
                     EELDDANLISAALDALGSETQRSDPAGARRIAERRVAMSDRLSMQERSDAYYMVAWMS
                     AVLGELPAVLTAAEGWLGQLQPGQGPAFAVGLSGWRAYALWQLGRWDEVAAAFDQMRS
                     LWLEANRIAAAFALQGMYAAMEVGRARGDAALVDRAKEMFDETVGRFDKSHPTRALAA
                     LGVPDPARIAADIIPRAMQYLERPVHVETALAICSDRAYRLDVGALQPLVDFAESAGV
                     RLVGAQAHRAIGISTNDSAALEKALGLFVEVGSLPFAARVRTEIGLLSGNSEMLERGL
                     AELDAYGDRDQLRRINERRTRSST"
BASE COUNT         1189 a         2712 c         2572 g         1213 t
ORIGIN      
        1 gagtagatct gcatcgactc gtggatgccg tactcggcac agaagtacgc aatcggacca
       61 gggagggtcg cagacgggtc cgagtccgtc gctgcccgat accaggagtc cgacccgttg
      121 gcgaggtagc gttcgaattc gtccaggacg cggctggcat ccaccatgaa gtcctcgtcg
      181 gccagcagct cggtccatcg gcccggctcg accgcggcga ggacggggat cgggctccgg
      241 tagcgcgacc aggccgcgga atcgatccgc gcaaagagcg cccctgcccg tggggtccag
      301 ctccaccaca ggttgtacgc gagttcgccc aggccttcga gcgcgggcgg gagcccgaaa
      361 tcgtagcctg gctgactggg cacgcggacc gcggaagtca tcgagggccg atcataggga
      421 gtcagaactg catcccgccg cttcctcgcg cgaaacatgc cgcaggtatc gccgtttttg
      481 gtgtcagcga gtaccctatc ggacccgtga tggacggcta ctcggtgcag gaggcagcgt
      541 cagtcctcgg ggtcgccgaa gggcgcgtct gggagctcct tgccagaggc gttctgctcg
      601 gcactcccga gggtgattcc atgcgcgttt tcctgaaggc gcaggccgga ccgatcgcgt
      661 ccgcaggcac ccccgacgaa ccgccacgca cgaatggcaa cggaggctcg cacgggccag
      721 caggcgaagc cagtgccttc cgggagctgc tgaccgaatt tcgcaacctg accgagcgat
      781 acggccaggc actgctcgcc ctgggggagg cgcgcggcga ggtggcgggg cttcgttcgc
      841 gcgtcgatca gctcgaggcg cgcctcgacc ttcgattgcc accgccagtc gatgctgctc
      901 cggtcgcctg ggattcgctg agcgttcccg ccaccacgcc tgagaaggca cgcgcaatgc
      961 ctccgtctcc cgcgccccgt gcgccggttc gacgcaagct gaagccgaag gcgcgccaac
     1021 gcaaggcacg ctctccgcgt gaggccctcg ccggctctcc ccgcgaggca gtggccggat
     1081 tcgccgaggc gctggcgcgc gcccaggatc cgaacgccgc ggacctcggg gaggctcggg
     1141 tggagacgga gcccctctcc gaggcgaaca ttgagcctgc cagcgcgacg gcggtagcgc
     1201 ccgacgagcc gatgcaggag gccgccgaca ccccggagct cgtagcccgg gaggtcggtt
     1261 cggacgagat tcgggaagat gagcttgctg ctgaggaggc cgcacccgcg ccggttgcta
     1321 ccgcgcagcc gactgcggag ctcgtggtcg aggaagcctc acccgacgaa ccgaccgccc
     1381 aggtcgaggc gccgacctac agcgccgtgg tggtggagcc agactggttc gcggacggcg
     1441 acttcgcgtg gcttgacgcg gcggagatgg aggggagcgc cgaggcgagt tccgccacgg
     1501 cgacgccaga taaggccgag caggagcccg aacaggcggc tcctgaggcg gaggcgccag
     1561 cagccgagtc cgagccggag gtccaagcag ccgagtccga gccggaggtg caagcagccg
     1621 agcccgagcc ggaggtgcaa gcagccgagc ccgagcccga gcagcagcaa ttgcaagagg
     1681 cgcaggtcgc ccccgagccg cctccccagc ccgagaccgg gttcgggccc tggcagccgc
     1741 ctgagccgaa gcccgtggaa tcgcaggacc cggagatcgc cgcggagccg gacctcgagc
     1801 gcgagcctcc gtcgcagttc gaagctgagg ctgagttcga gtcctggcaa ccgcccgagc
     1861 cagcttcccc ggaggctcct gctgagccgg aagtcgcgga tgcgccggaa ccgccgacgg
     1921 cgagcgcggc cgtcgcggtg gaagaggtcg aggaagaggt catgtggctc ggccgcgagt
     1981 cggacccgag cccggtcatc cacgatgagc ccagtccgga ctaccgcgcc gaggcagagg
     2041 agtggcccag gatcgatgcc gctcccacga tggaggcggt acggccaccc ctcgccatga
     2101 ccgaggatga gctcgctcga ctcgcgctcg acgagggctg ggacgacacc gaagtggcag
     2161 ccatccgcgc gatgatcacc ccgccggcga tgtcagcggt cgatctgcct ggggccgcgg
     2221 agctccacga ggcgatggct gcgcttgagg cggtgcctgt ccagaccgat cccccttacg
     2281 acgcatcccg ccagtgggcc aagccggcgc cggacgacga ggagccagcc cgtcacgacg
     2341 attgggcgtt cgaggccgag cccgccccgc cgccgccggc tcccacgcgt cctccgagtg
     2401 acgtgacttc cgcgttgcgg cgcgccctcg aagaccccgg ctggctgcgg cgtcggcagg
     2461 gcccggcggc ggaggcctac cgcaggctgc ggcgcctctt cccgggttga cgggccgcgg
     2521 cgcgactccg ccgcgtcggg gacaccttcc ggatctcggg cctcacggcg aaggatgggt
     2581 cgtcggtcag gtggccctga tcggcctggt cgtcgtgctg ggctcgccgg ggctgcgaga
     2641 catttccccg cacatcgcct ccggctgggt cggtctcctc gtcggcatgg cggcgatcgt
     2701 catcggcgcc tggttggtga tccgcgctgt cgccgacctc ggccggagcc tgagccccat
     2761 gccgcgaccg cgacctgatg ggcggttgat cgacacgggc atttatgccc gcctgcggca
     2821 tccgatctat gcgggggtga tcttcgcgag ctttggctgg agtgtgctca ctgggagcct
     2881 cggcgcgctc gtcgcggcgc tcctcctggc catcttcttg gacgccaagg cccgccgcga
     2941 ggagatctgg ctgctcgagc gctacgacgc ctacgcggac tatcggcaga ggtcgaagcg
     3001 gttcctgccg ggaatctact gagcgtcatc tccccggccg cttgcgctag aagcttcgtg
     3061 tctgcataat gattcgcgtg acacgaacta ttcacgacac gagaactgtc gccgctgatc
     3121 tgctcgacga gcttggcccg atcatcgccg gcgagcgggc cgcgttcgcg cttcgatgcc
     3181 accaccgttc catcagcatg acccatctgc tactgatgac ccttctcgag acgcacggcc
     3241 cgctgccgat gagccgcgtc gccgagctcc tcggctgcgg ccttcccgct gcaaccggga
     3301 tcgtgtcccg catgcaggag cgcgggctgg tggaacgcca gcatgactcc gaggaccgtc
     3361 gcgtggtgac gctcagcctc tccgacgagg gtgctgtcga gatccgcgag ctccacctca
     3421 gccgtcgtga gcgcatggcg acggccctca cccacctctc cgagcctgag cgcgagcagc
     3481 tcctggcctc ggttcgatcc ctccgtgcgg cgttcaaccg cgcgaattcc caaggagaca
     3541 acgcgtgacc gccgccgccg agccaatgga cgcacccgtc cacgtcgatc cccgcgccag
     3601 gaccgagatc ctgatcgcca tcctgctggc aatgttcctg tccgccctcg accagacgat
     3661 cgtcggcacg gcattgccga cgatcatgac ccagctgcac ggcaacgact actacacctg
     3721 ggtggtgacg atctacctcc tgaccgccac catcagcgga ccgatctacg ccaagctctc
     3781 cgacctcttc ggacggcgac cgatggtcat gatcggcgtc accctcttcc tcatcggctc
     3841 ggcgctgtcg ggcctctcgc aggaggcgtg gcagctcatc gccgcacgcg gtctgcaggg
     3901 ccttggtgcc ggtgccatct tcccgatcgc cctggccgtc atcggcgacc tgttcacccc
     3961 cgccgagcgc ggcaagtacc agggcctctt cggcgcggtc ttcgggatca gcgccctcat
     4021 cggtcctgcg ctggggggct tcctgaccga caacgtcggc tggcactgga tcttcttcgt
     4081 gaacctgccg atcggtgccg tggcgctctt catcatctgg cgcctcctgc cggcgatcaa
     4141 gcatccggag cgcgcgcgca gcatcgacta cctggggagc gtggtcttca ccgccgccct
     4201 ggtgccgatc ctgatcggcc tcaccaacaa ggcgacgatg gactggaccg acccgcaggt
     4261 cggcggcctg ctcctggtcg ggttcgtcct tgcggcggtc ttcgtgtggg tcgagtcgcg
     4321 ggccagcgag ccgatcctgc cgctgagcct cttccgcaac cgatccatcg ccgtctccct
     4381 catcgccgtc ttcctcgccg ggttcggctt cttcagcgcg atcatcttcg tcccgcgctg
     4441 gttccaggtg gtcctgggat ccagcgccac cgagtctggc taccagattc ttccgctgct
     4501 ggccggattg atcatcagct cggtcatttc ggggcaggtc atcagccgga ccggccgcta
     4561 tcgctggatc atcgtgatct ccatggcctt cctcgccgtc ggcctactgc tgatgacgca
     4621 gctgcgggcc gagaccagcc ttcccgagat gtggttctgg atggtcgtgg cgggcatcgg
     4681 gatcggcccg accctggccg ctttcaccat cgtcgtgcag aatgccgcgc cgtttgcgca
     4741 gctgggtgcg gcgacgggtg cgctgacctt cttccgccag gtgggcggca cggtcgggct
     4801 cgccatcggt ggcacgctgt tcggttcggc gctggtgacc gagatcccgc agcagatggc
     4861 caaggccgga gtcccgtccg acatcgccgc acgcttcgcc acatcgggag tggctactga
     4921 tgtcagctcc gttggcgacc tgggcgcgcg gatcctgaag gccgtgccgg aacaggtccg
     4981 gccgctggtc gatccctaca tccccaagat cgtcttcgcc ttccacgagg cattcagcct
     5041 ggcgatcgcc agcgccctgt gggtcagcct ggcggccgca gtgattgcgc tggtggtggt
     5101 aaccatcgcg cttccggagc tgccgctgcg gacgcaccac ggtcctgcgc gcgcgggtgc
     5161 gcgcccggag gatggctcgg agccagcacc gatcgcggct ctggactgat cgcgcctcag
     5221 gtgctcgaac gggtgcggcg ctcgttgatc cggcgcagct gatcgcggtc accgtacgca
     5281 tcgagctcgg ccaggccgcg ctcaagcatc tcgctgttgc cgctgagaag gccgatctcc
     5341 gttctcacgc gcgccgcgaa cgggaggctg ccaacctcca caaacaggcc caacgccttc
     5401 tcgagcgcag ccgagtcgtt ggtgctgatg ccgatcgccc ggtgcgcttg tgccccgacc
     5461 aggcgcacgc cggcactctc ggcgaaatcg acgagtggct gaagggcccc tacatccagg
     5521 cgatacgcgc ggtccgagca gatcgcgagg gcagtctcga catggacagg ccgttcgagg
     5581 tattgcatcg cccgcggaat gatgtccgcg gcgatgcgag ccgggtctgg gaccccgagc
     5641 gccgccagcg cccgggtggg gtggcttttg tcgaatcggc caaccgtttc gtcgaacatt
     5701 tccttcgccc ggtccaccaa cgcagcgtca ccccgtgccc ggcccacctc catcgcggca
     5761 tacatgccct gcagtgcgaa ggcggcggcg atccggtttg cctcaagcca tagcgagcgc
     5821 atctgatcga aggcggcggc gacctcgtcc catcgcccga gctgccagag cgcataggct
     5881 cgccatccgg acagcccgac cgcgaacgcc ggtccctgtc cgggctgaag ctgtccgagc
     5941 cagccttccg ctgccgtcag gacggctggc agctcgccga ggacggccga catccacgca
     6001 accatgtagt acgcgtccga tcgctcttgc atcgacaaac ggtcggacat cgccacgcgc
     6061 cgctcggcga ttctccgggc gccagccgga tccgatcgct gcgtttcgct gccgagcgcg
     6121 tcgagcgcgg ccgagatgag gttggcgtcg tcgagctcct cggcgagctg catgcccgcc
     6181 tgggcgctcg ccattccgtg agcgatctcc tcggctgacg gctggcggac gccgctgttc
     6241 cgcagccaga aggcgacgaa gccctccgag atgagaaata ccgccttggc ccgccggtcc
     6301 ttggccacct cgaacagggc acgggcttcg tcgcgcagcc gtgcgaagtc gtcctcggca
     6361 ggctggcccg ccacggacgc gtaccagcgg ctcatcacga acagcatttc gccaagcacc
     6421 cgcagcagga agtcaggtgg gcgacccgcg tctcgtccca gccgcagcgc ggtggcatac
     6481 gcctccactg actggtcccc ggccagatac gtgtcgccca ggcgtccgta cagctctggc
     6541 agctgcgtcg gatcggccaa ttcgatggcg gccagcagat gccgactggc ctcaacgttg
     6601 gccatcgacg cgaacgccac ctcgcccgcc cgccgcagcc attcgaccgc cgccgcatca
     6661 atcgcgacgt cgcgcatccc cgtcgatgcc gtgagggcag ccgcctcgcg caggtggaag
     6721 gcaatcagct ccgctagttc atcttcccgt ccagccgatg tcgccgccaa ccagtgaccc
     6781 gccgcggcat gcagccgtgc gcgctcgctg cgaggcagcg tcgagtacgc gacttcacgg
     6841 atcaggatgt gccgatagct gaatcgatcc cctcctgacg gccgcacgag gtcgcgctcg
     6901 acgagccgtt cggcggcgcc cgcaaggtca agcttcgcct ccggctcgag cgcctggacg
     6961 ccggccagcc gaaatgaacg accatgaacc gatccaagct ggaggagacg gcgggccggt
     7021 ccgtcgagca ggtcaaggcg tgcgagcacc gtcgcctgga ccgtgtccgg cagggtcgca
     7081 atcgcggcct gcagcgccac ggggtcgtcg agcgcagccg cgcgctcgcg gatcgagcgg
     7141 acgagctcgc caacgtagaa agggttgccc tctgcccgtt cgacgacaag ccgcaccagc
     7201 tccggcgcag gcccctccaa gatcagctcg acaaggcggc tgacctcgtt cggttcgagc
     7261 ggatcaagcg ccaacgagac gtggttgcgt cgaccgccgc cccacgacgg acgccggtcc
     7321 aagagctccg gtcgcgtgag caccagcatc agcagtggag catcggtccg gggctggagc
     7381 acgtactcga tcaggtccaa caggctctcg ctcgaccaat gcaggtcctc gatcaccagg
     7441 acgagggggt tgtcggcggc cgccagctcg acggtgtggc gccaggcggc gaagatatcg
     7501 ccgcgatcga ccacctcggc atccgctgag ccgatggttg caatcagccg ctcagcggtg
     7561 cggtcggcgt cctcgtcgcc gacgtcgcgc agccagcgtc gcgcctgggt ccgtatctca
     7621 tcggccgcgc tctcgtcgcc gaggtcgagg agctggagca ggagcgagcg catcggagcg
     7681 taggtc
//
LOCUS       DATCQR010000124         6124 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_7411, whole genome shotgun sequence.
ACCESSION   DATCQR010000124 DATCQR010000000
VERSION     DATCQR010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6124
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7411"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="VIN32_04170"
     CDS             <1..226
                     /locus_tag="VIN32_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013132959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEY8921809.1"
                     /translation="VLALWGGADQGIGPGDVQAFDEALDRAGVLHTTVTYSGAPHSFF
                     DRHASEYAEASADAWRQMLDFMRVRGTATG"
     gene            complement(230..1117)
                     /gene="efeU"
                     /locus_tag="VIN32_04175"
     CDS             complement(230..1117)
                     /gene="efeU"
                     /locus_tag="VIN32_04175"
                     /inference="COORDINATES: protein motif:HMM:NF015213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HEY8921810.1"
                     /translation="MDIGFLTTGLLTGLREGVEAALIVSIILAYLARTGNARRFGRIW
                     VGTGAAIALSAAVGGVLWISIGGLTSPAAQYFEGSAMLLAAIVVTWMLFWMRRTAANI
                     KGELQAGVDRALTGGSVWALSLLAFTAVIREGVETSLFLLGQATAATTEQAGAASTLV
                     GAFIGILIAALLGYGFYRGARVINLARFFRWTGVALVFIAGGLVSVAVHEFIDAGLIG
                     FGTSTAFDISAVLPDAPEGGNLLGQLLRAVFGYSSTPEWTTLLTWVVYVIGVLALYLQ
                     PMKPRKPAPVSSSLPAAGG"
     gene            1234..2430
                     /locus_tag="VIN32_04180"
     CDS             1234..2430
                     /locus_tag="VIN32_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY8921811.1"
                     /translation="MIDFSLTDAQREVQRTARVFAEREILPRIHELDSTSTYDRSLYE
                     KMGAAGLLGLPIPERYGGTGMDYIAFALLCEEMERADTAFRVILSVHTGLNSLTLLQW
                     ANEDQKQRYLVPQARGEKLATFGLTEPGVGSDAANLTTTARRDGDRYLLNGSKIWISL
                     ADTADNFLVFATVDKSAGHKGITAFVVERGFAGFSTDSLHGKLGVRAGNTGILTFSDC
                     EVPIENRIGEEGEGFSIAMSAIDQGRYTVAAGSVGLAQACLDASVKYAHERKAFGEEI
                     GRHQLVKQMLANMYAGIEAGRLLVFKAGWKKNAGERNTRETSLAKWFCTDHAVQSAMD
                     AIQIHGAYGYSDEYPVERYLRNSKGSVIYEGTSQIHTLIQADYLLGYRQDHALRMPQP
                     PFEGEM"
     gene            2436..2981
                     /locus_tag="VIN32_04185"
     CDS             2436..2981
                     /locus_tag="VIN32_04185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921812.1"
                     /translation="MSRAVELLLGLMLLVIGLLVLASEPLGRWFAGLGISEELLAWWP
                     AIVVVLSGFFLVPAAFPGPNRRLRAGMVIPGAFLAGVGGTLLYTSVNDRWDAWFYLWT
                     VVPFSIGIGLYAAGWIADLPALKWIGSGMAVGAAIAYLAFATAFGGETFRLVGSLVII
                     LLGLALVAGGLADRLSRKGSR"
     gene            complement(2978..3514)
                     /locus_tag="VIN32_04190"
     CDS             complement(2978..3514)
                     /locus_tag="VIN32_04190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921813.1"
                     /translation="MTPPTDDEMVPVSVRLGAVVPPEDPEDWTRPLTWVAAIGMLAAP
                     AVALAWYAVAAPHDGTRLAPGTWAVAATLVIGAAAAGATQIGRLRAFTGTLGAALLGA
                     VLVVAAGAVTAGERQLGSASPTVAHSLAGAIAGLAGALPASVAGALTARSWPRLRRGA
                     LAGALGLLAMATILPRLF"
     gene            complement(3511..4770)
                     /gene="cysS"
                     /locus_tag="VIN32_04195"
     CDS             complement(3511..4770)
                     /gene="cysS"
                     /locus_tag="VIN32_04195"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEY8921814.1"
                     /translation="MTLRLFDARRAELAPFAPIGPAPVGLYVCGVTPYDTGHLGHAFT
                     YLSFDVLRRYLEFLGHGVFYVQNVTDVDDDMLRRARETGEDYLELGNRHLTTFLTEMA
                     ALNWLPPDELPRATEHIPQMLEQIERLVANGHAYADAGHVYFSVDSCPTYGELSKFSR
                     EDMLPLALERGSRPEMPGRRDPLDFVLWQPSLVDEPAWESPWGPGRPGWHIECSAMGI
                     AYLGSHFEIHGGGGDLIYPHHESEIAQAEAATGQRPFVEWWMHAGMLRYDGEKMSKSL
                     GNLVLARDLLRSFSGDAIRLYLVSHHYRSELAYRQADLEAAAVSAARLRMASLAAAQG
                     DADPATLGPVVRQHRDRFLDAMDNDLDTPSAVPELLAIADLALESDDAGERGDAGRTV
                     RELGTRILGLRLASVPVPPEMGQAVGT"
     gene            complement(4970..5467)
                     /locus_tag="VIN32_04200"
     CDS             complement(4970..5467)
                     /locus_tag="VIN32_04200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921815.1"
                     /translation="MGRHDATAPFDEGSLRLNWFPLLLAAHVVLAVSLLAPSLVLPFL
                     LRRRPASGEPSTIIRLLAALQGTGSVVIAIGLALTGAGLVLSLGTEILTRPWLLVALA
                     VYAANLLVAAFVSRPSLRRLMRVREPESEGWRQQARRVRYMAYAMAVTTGLIGFLMST
                     KPALW"
     gene            5466..>6124
                     /locus_tag="VIN32_04205"
     CDS             5466..>6124
                     /locus_tag="VIN32_04205"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8921816.1"
                     /translation="MRLRPYLTLLRDNPAFTRLFAAQLISFAGDWFATVALLGLALQL
                     TGSPAVASLLLVVQTGAFALASPIAGVLADRYDRRRLMVIADVARVPVALAFLLARDP
                     DTLWIAFAAVALLSLGAAIFEPTSSASLPNLVEEGELAEANVLIGSAWGTMLAVGAAL
                     GGLVAATLGRDVAFAVNAASFGASALLIVGIRRSFRAAREDQRPGERANEEGGGVAES
                     IR"
BASE COUNT          945 a         2096 c         2153 g          930 t
ORIGIN      
        1 ggtgctggcg ttgtggggcg gtgccgacca gggcatcggt cctggggatg tgcaggcctt
       61 cgatgaagcg ctggatcgtg cgggcgtgct ccacaccacg gtcacctatt cgggagcgcc
      121 ccattcattc ttcgatcggc atgcgagcga gtatgccgag gcatcggcgg atgcctggcg
      181 ccagatgctg gacttcatgc gtgtcagggg caccgcgacc ggctagggct catcctccgg
      241 cggcgggcag gctgctcgag accggggcgg gcttccgcgg cttcatcggc tgcaggtaga
      301 gagccagcac accgatcacg tacacgaccc atgtgagcag ggtcgtccac tccggggtgc
      361 tgctgtagcc gaacacggcg cgcagcagct gtccgagcag gttgccgccc tccggcgcgt
      421 caggcagcac ggcgctgatg tcgaaggcgg tcgaggtgcc gaacccgatc agccctgcgt
      481 cgatgaactc gtgcaccgcg acgctgacca gtccgccggc gatgaagacg agcgcgactc
      541 cggtccatcg gaagaagcgc gccaggttga tcacccgcgc gccgcggtag aagccgtacc
      601 cgagcagggc ggcgatgagg atgccgatga aggccccgac cagtgtgctc gctgccccgg
      661 cctgctcggt cgtggccgcg gtggcctgac cgaggaggaa gagcgacgtt tcgaccccct
      721 cgcggatgac cgcggtgaag gcaaggagcg agagggccca gacgctgcca ccggtgagcg
      781 cgcgatcgac gccggcctgg agctcgccct tgatgttggc agcggtgcgt cgcatccaga
      841 agagcatcca ggtcaccacg atggcggcga ggagcatggc gctcccctcg aagtactgcg
      901 cggccggcga cgtgaggccg ccgatgctga tccacaggac gccgcccacc gctgcgctga
      961 gcgcaatcgc ggcgccggtg ccaacccaga tgcgcccaaa gcgacgggca tttccggttc
     1021 gggccaggta cgccaggatg atgctcacga tgagcgccgc ctcgaccccc tcgcggaggc
     1081 cggtgagcag cccggtcgtc aagaagccga tatccaaggg aaatcaaggc tagccgagcg
     1141 ccgtttgccc gtcaatcgaa gtcctaccgg gtgagtcagg attcgcgcca cgagggcggg
     1201 gaggaggcgt cggacggcta gaatccgagc ctcatgatcg acttttccct gaccgatgcg
     1261 cagcgcgagg tgcagcgcac ggcccgtgtc tttgccgagc gagagatcct gccgcgcatc
     1321 cacgagctcg actcgacctc gacctacgac cgctccctgt acgagaagat gggagccgcg
     1381 ggcctgctgg gcctgccgat ccccgagcgc tacggcggca cgggcatgga ttacatcgcc
     1441 ttcgcgctgc tgtgcgagga gatggagcgg gccgacaccg cgttccgcgt catcctctcg
     1501 gtccatacgg gcctgaactc gctgacgctg ctgcaatggg ccaacgagga ccagaagcag
     1561 cgctacctcg tcccgcaggc ccgcggcgag aagctggcca ccttcgggct gaccgagccg
     1621 ggggtgggga gcgacgcggc gaacctgacg accaccgcgc gccgcgatgg cgatcgctac
     1681 ctcctcaacg gcagcaagat ctggatcagc ctggccgata cggccgacaa cttcctggtc
     1741 ttcgccacgg tcgacaagag cgccggccac aagggcatca ccgccttcgt cgtcgagcgc
     1801 ggcttcgccg gcttcagcac cgattcgctg catggcaagc tcggcgttcg cgccgggaac
     1861 accggcatcc tcaccttctc cgactgcgag gtcccgatcg agaatcgtat cggcgaggag
     1921 ggcgagggct tcagcatcgc catgagcgcg atcgaccagg gccgatacac ggtggcggcc
     1981 gggtcggtgg ggctggcgca ggcctgcctg gacgcatcgg tcaagtacgc gcacgagcgc
     2041 aaggcctttg gcgaggagat cgggcggcac cagctggtca agcagatgct ggcgaacatg
     2101 tatgccggca tcgaggcggg gcgcctgctc gtcttcaagg ccggctggaa gaagaatgcc
     2161 ggggagcgca acacgcgaga gacgagcctg gccaagtggt tctgcacgga ccacgccgtg
     2221 cagagcgcga tggacgcgat ccagatccac ggcgcctacg gctacagcga cgagtacccg
     2281 gtcgagcgct acctgcgcaa tagcaaggga tcggtcatct atgaggggac ctcgcagatt
     2341 cacaccctga tccaggccga ttacctcctc ggctaccgcc aggaccacgc cctgcggatg
     2401 ccgcagcccc cgttcgaagg cgagatgtag gccccatgag tcgagctgtg gagctgctgc
     2461 tggggctgat gctgctcgtc atcgggctcc tggtcctcgc aagcgagccg cttggccgct
     2521 ggttcgccgg gcttggcatc agcgaggagc tgcttgcctg gtggccggcg atcgtggtcg
     2581 tgctgtccgg cttcttcctc gtcccggccg cgttcccggg tccaaatcgc cggctgcgtg
     2641 ccggcatggt gatccccggc gcgttcctgg ccggcgtcgg aggcacgttg ctgtacacca
     2701 gcgtcaacga ccgatgggat gcctggttct acctgtggac ggtcgtcccg ttctcgatcg
     2761 gcatcggcct gtacgcagcc ggctggatcg ccgacctgcc ggccttgaag tggatcggct
     2821 cgggaatggc ggtgggggcc gcgatcgcct acctggcctt cgcgaccgcc tttggtgggg
     2881 agacgttccg attggtcggc tcgctggtca tcatcctgct ggggctggcg ctggtcgccg
     2941 gcggcctggc cgatcggctg agccgcaagg ggagccgcta gaagagccgg gggaggatgg
     3001 tcgccatcgc cagcagcccg agcgctcctg ccagcgcgcc tcgtcgcagt cgaggccagg
     3061 atcgcgccgt cagtgcgccg gccacggacg cgggcaatgc tcctgccagc ccggcgatgg
     3121 cgcctgccag cgaatgcgcc acggtcggcg atgcggagcc gagctgccgc tcgcccgcgg
     3181 tcaccgctcc ggccgccacc accagtacgg cgcccagcag cgcggcgccg agggtcccgg
     3241 tgaaggcccg caaccgcccg atctgggtcg cgcccgccgc cgcggcgccg atcaccaggg
     3301 tggccgccac ggcccacgtc cccggggcca gccgcgtgcc atcgtgcggg gcggcgacgg
     3361 cgtaccaggc cagggccacc gccggcgcgg ccagcatccc gatggctgcc acccacgtca
     3421 gcggtcgcgt ccagtcctcg gggtcctcgg gcgggacgac ggcacccagt cggacactga
     3481 cgggaaccat ctcgtcgtcg gtcggcggcg tcacgtcccg accgcttggc ccatctccgg
     3541 cggcaccgga acgctcgcca gccgcaggcc gaggatccgc gtcccgagct ctcgcaccgt
     3601 ccgaccggcg tcgccgcgct ccccggcgtc gtcggactcg agcgccaggt ccgcaatcgc
     3661 caacagctcc ggcacggccg agggcgtgtc caggtcgttg tccatggcat ccaggaagcg
     3721 atcacggtgc tggcggacca cggggccgag ggtcgcggga tcagcgtcgc cctgagctgc
     3781 cgcgaggctg gccatccgca gccgcgcggc gctgacggcg gccgcctcca gatcggcctg
     3841 tcgataggcg agctcgcttc ggtagtggtg gctgaccagg tagagccgga tcgcgtcgcc
     3901 gctgaaggag cgcagcaggt cgcgcgccag gacgaggttc ccgagcgact tgctcatctt
     3961 ctcgccgtcg taccgaagca tgccggcatg catccaccat tcgacgaagg ggcgctgccc
     4021 ggtcgccgcc tcggcctgcg cgatctccga ttcgtggtgc gggtagatca ggtcgccgcc
     4081 accgccatgg atctcgaagt ggcttcccag gtaggcgatc cccatggcgg agcactcgat
     4141 gtgccagccc ggccggcccg gcccccacgg cgactcccat gcgggctcgt ccaccagtga
     4201 cggctgccac agcacgaagt cgagcggatc gcgccggccg ggcatctccg gccggctgcc
     4261 gcgctccagc gccagcggca gcatgtcttc ccgcgagaac ttcgacagct caccgtaggt
     4321 cgggcaggaa tcgacgctga agtagacatg cccagcgtcc gcgtaggcgt gcccgttcgc
     4381 taccagcctt tcgatctgct cgagcatctg ggggatgtgc tcggtcgcgc ggggcagctc
     4441 atcgggcggc agccagttga gcgccgccat ctcggtcagg aaggtggtca ggtggcggtt
     4501 gcccagctcg aggtagtcct cgccagtctc gcgcgctcgc cgcagcatgt cgtcgtcgac
     4561 atcggtcacg ttctggacgt agaacacgcc gtggccgagg aactccaggt accgacgcag
     4621 gacgtcgaag ctgagatagg tgaatgcatg gcccaggtgg ccggtgtcgt acggcgtcac
     4681 gccgcagaca tagaggccca cgggggcggg gccaatgggg gcgaatggcg cgagctcggc
     4741 acgccgagca tcgaacaggc gcagggtcac gggcgggatt ctaggcgctg ctagaatcgg
     4801 ggggccgacc gccaggggat ggggtcgagc gggagcagca tcggagccaa ggccgtgtgg
     4861 gacgttctgc gctggttcgc cgtcttcgtg attgccatcc tggtgatcgt ggcggggctc
     4921 ttttggatat tcggcaggcc actgccgatt ccacagctct tctgaccact caccagagcg
     4981 cgggcttggt gctcatcagg aagccgatga ggccggtcgt gaccgccatc gcataggcca
     5041 tgtagcggac ccgcctggcc tgctgtcgcc acccttccga ctccggctcg cgaacccgca
     5101 tcagccgccg caggctcggc cgcgagacga aggccgccac cagcaggttc gcggcgtaga
     5161 ccgccagcgc gaccagcagc catggacggg tcaggatctc ggtccccagg ctgaggacca
     5221 gcccggcgcc ggtcaatgcc aggccgatgg cgatcaccac gctgccggtc ccctggagcg
     5281 ccgccagcag gcgaatgatg gtgctcggct cgccgctggc agggcgacgg cgcaggagga
     5341 agggaaggac caggctgggt gccagcagcg acaccgccag gaccacgtga gccgccagca
     5401 ggagcgggaa ccagttcaag cggagtgagc cctcgtcgaa tggcgccgtg gcatcatgcc
     5461 gacccatgcg cctgcggccg taccttaccc tgctgcgcga caatcccgcc ttcacccgcc
     5521 tcttcgccgc ccagctgatc agcttcgccg gcgactggtt cgcgacggtc gcgctgcttg
     5581 ggctggccct ccagctgacg ggctcgccgg cggtcgcctc gctgctgctg gtggtgcaga
     5641 ccggcgcctt cgcgctcgcc tcgccgatcg cgggcgtcct tgcggaccga tacgaccggc
     5701 gccgcctgat ggtcatcgcc gacgtggcgc gcgtcccggt ggcgctggcc ttcctcctgg
     5761 ctcgtgaccc cgacacgctg tggatcgcct tcgcggcagt cgcgctcctc tcgctgggcg
     5821 ctgccatctt cgagccgacc agctccgcgt cactccccaa cctggtcgag gagggtgagc
     5881 tggccgaggc gaacgtcctg atcgggtcgg catggggcac gatgctggcg gtcggcgccg
     5941 cgctcggcgg cctcgtcgcg gcgacactgg gacgggacgt cgcattcgcc gtcaatgcgg
     6001 cctccttcgg cgcctctgcc ctcctcatcg tcgggattcg acgctccttt cgggccgcgc
     6061 gcgaggatca acggccgggg gagcgggcca atgaagaggg tggcggagtc gcagagtcga
     6121 tccg
//
LOCUS       DATCQR010000125         2398 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_38112, whole genome shotgun sequence.
ACCESSION   DATCQR010000125 DATCQR010000000
VERSION     DATCQR010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38112"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..679
                     /locus_tag="VIN32_04210"
     CDS             <1..679
                     /locus_tag="VIN32_04210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921817.1"
                     /translation="DMLDALRNAERDLFAMVPADKREAAGTIGEWSPKDLLAHLGAWR
                     AVEARRLDARASGGAASPDDPGLDEPIDESNARLQARDRDLSWGTVAAEADASIDALI
                     TAVDRSSTDALCECDDASVAGIGVNGVNHAVGHLPEIAGLVGGGARFDAFARQVETVL
                     RAGHVPPRDSGTILYNIACARALSGELDDARRLLAAAFTRRHDLADLATHDPDLVALG
                     GELSSPA"
     gene            complement(676..>2398)
                     /locus_tag="VIN32_04215"
     CDS             complement(676..>2398)
                     /locus_tag="VIN32_04215"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015655856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEY8921818.1"
                     /translation="LDPGRARRRFALRADRRDEPLMRAADLVFLGATVEPITPVAGPA
                     PEALAVTDGRISAVGRGADVEQLIGAATRVVRMDGETLLPGFQDAHIHPVQGELTVMQ
                     CDLSELSADRFDAAIRAYAAQHPDCEWIVGAGWSVTDFPDAAPRRADLDGLVPDRPAF
                     LWSRDGHSAWVNSRALDLAGISAATPDPAGGLIVRDPGGSPSGTLREGAVSLVEAVVP
                     PLSAEDHATALASVQRRLHALGITAWQDASVYDSGACARQLDAYRAAAASGSLQARVV
                     AALFWDPRRGLEQVEGFVEQAAAAGIGRLRAATVKIWVDGVIEGLTAAMLDPYLDEHG
                     NATDDRGMALVAPEALHEAVTALDRAGLQVHMHAIGDAAVRSALDAIEAARHANGPSD
                     GRHHIAHVQLVHPADLPRFAALGATANMQPIWACHEPTMDQRTIPFLGPERTSWQYPF
                     ASLLQHGARLAAGSDWPVSSANPMLEIEVAMTRVSELSRSGDPFLPDERLGLDQALAA
                     FTIGSAYVSHLDAETGSIEVGKLADLALMDRNLRHPEAGPIGEASVQATWVEGLEVYA
                     KPMLAPG"
BASE COUNT          341 a          839 c          880 g          338 t
ORIGIN      
        1 ggacatgctc gatgccctgc gcaacgcgga gcgggatctg ttcgccatgg tcccggccga
       61 caagcgggag gccgctggca cgatcggcga atggtcgccc aaggatctcc tggcccacct
      121 cggagcctgg cgtgccgtcg aggcgcgacg gctggacgcc cgagcgtcgg gtggtgcggc
      181 cagtcccgac gatcccggcc tggatgagcc gatcgacgaa tcgaacgcca ggctccaggc
      241 gcgcgatcgg gacctgagct gggggacggt cgcggccgag gcggatgcga gtatcgacgc
      301 gctcatcacg gccgttgacc ggagctcaac cgatgccctc tgcgagtgcg acgacgcatc
      361 ggtcgccggg atcggtgtga acggcgtgaa ccatgcggtc ggccacctgc cggagatcgc
      421 cggcctggtc ggcggcggtg cgcggttcga cgcgttcgcg cgccaggtcg aaacggtcct
      481 gcgggcgggc cacgtgccgc cgcgcgactc cggcacgatc ctctacaaca tcgcctgcgc
      541 ccgggcgctg tcgggtgagc tggacgacgc gcgccgtctc ctggcggcag ccttcacccg
      601 tcgtcacgac ctggccgacc tggccacgca cgatccggac ctggtcgccc tgggcgggga
      661 gctgtcgagc ccggcctagc cgggcgccag catcggcttc gcgtagacct ccagcccttc
      721 gacccaggtc gcctgcacgc tggcctcgcc gatcggaccc gcctcaggat ggcgcaggtt
      781 gcgatccatc agcgccagat ctgcgagctt gccgacctcg atcgacccgg tctctgcatc
      841 gaggtggctc acgtacgccg agccgatggt gaatgcggcc agggcctggt ccaggccgag
      901 gcgctcgtcg ggcaggaatg ggtcgcccga gcgggagagc tcgctgaccc gagtcatggc
      961 gacctcgatc tcgagcatcg ggttggcgct gctgacgggc cagtcgctgc ctgcggccag
     1021 gcgcgcaccg tgctgcagga ggctggcaaa ggggtattgc cagctcgtgc gctcaggtcc
     1081 gaggaacggg atcgtgcgct ggtccatggt cggttcgtgg caggcccaga tgggctgcat
     1141 gttggcggtc gcgccgaggg ccgcgaaacg gggcaggtcg gcggggtgga cgagctggac
     1201 gtgggcgatg tggtggcgcc cgtccgacgg tccgttggca tggcgtgccg cctcgatggc
     1261 gtcaagggcg ctgcgcaccg ccgcatcccc gatggcgtgc atgtgcacct gcaggccggc
     1321 acggtcgagg gcagtgaccg cctcgtgcag ggcctccggc gcaacgagcg ccatgccccg
     1381 gtcgtcggtc gcattgccgt gctcgtcgag gtacgggtcg agcatggcgg cggtgagccc
     1441 ctcgatcacc ccgtcgaccc agatcttcac cgttgcagcg cgaagccggc cgatgccggc
     1501 ggctgcggcc tgctcgacga acccctcgac ctgctccagg ccacgacgcg gatcccagaa
     1561 gagtgcggcc accactcgcg cctggaggct gccactggcc gccgctgcgc ggtaggcgtc
     1621 cagctgtcgc gcacacgcgc cggagtcata gaccgatgcg tcctgccagg cggtgatgcc
     1681 cagcgcgtgg aggcgtcgct ggaccgacgc cagcgcggtc gcatggtcct cggcgctcag
     1741 tggcggcacg accgcctcca ccagcgagac tgccccctcc cgcagcgtgc cgctgggcga
     1801 gccgccggga tcgcggacga tcaacccgcc cgcggggtcc ggcgtggccg ctgagatccc
     1861 ggccaggtcc agcgcccgcg agttgaccca ggccgagtgg ccgtcgcggc tccacaggaa
     1921 tgcaggccgg tccgggacga ggccatccag gtcggcccgc cggggcgcgg cgtcggggaa
     1981 gtcggtgacg gaccagcccg cccccacgat ccactcgcag tcggggtgct gcgccgcgta
     2041 ggcgcggatc gcagcgtcga accggtccgc gctcagctcg ctgagatcgc actgcatgac
     2101 cgtgagctca ccctgcaccg ggtggatgtg cgcatcctgg aagccgggaa gcagtgtctc
     2161 gccgtccatg cgcacgaccc gcgtggcggc gccgatgagc tgctcgacgt cggctccgcg
     2221 gccgaccgcg ctgatccggc catcagtgac ggcgagcgcc tcgggcgccg gacctgccac
     2281 gggggtgatc ggctcgaccg tggcaccaag gaagacaagg tcggcagccc gcatcagcgg
     2341 ctcatctcga cgatcagcac ggagcgcgaa tcgtcgtcgt gctcgaccag gatcgaga
//
LOCUS       DATCQR010000126         4915 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_22979, whole genome shotgun sequence.
ACCESSION   DATCQR010000126 DATCQR010000000
VERSION     DATCQR010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4915
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22979"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="VIN32_04220"
     CDS             complement(<1..378)
                     /locus_tag="VIN32_04220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921819.1"
                     /translation="MSDARDFFGYKRPDDEPGRREETAEDDPTAPPQLDEAITLDSPR
                     VIKGIDSADMVGRVRELPRQLALARRVAAAVKLPTSHTEVDGICVLAMGGSAIGAELV
                     EGIAGERLRVPLVVHRDYGLPAWA"
     gene            complement(383..1837)
                     /locus_tag="VIN32_04225"
     CDS             complement(383..1837)
                     /locus_tag="VIN32_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014435197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HEY8921820.1"
                     /translation="MTTIKFGTDGWRAAIAEDFTFHNVRRCARGVAEYLAAEGTADRG
                     VVVCHDRRFASEYFAQACVEVLAAHDIRSFMTPDAVPTQVGSFFTREMAAGAGIVITA
                     SHNPWTDNGFKVKADTGGAAAPEMLAAIEATMDRHPEDELPPRRDYAEAVAAGLVATF
                     DPYPRFREQLASIVDLERIRAAKGRVLVEPMYGSGAGWFTRLLGGAGLTVNELHTERN
                     PFFGGVNPEPIRPNIDAWLEEIPRWGADIGIAFDGDADRVGMATEEGVFVNQLQVYGL
                     LYWYLLEVRGQIGPAVYTVTSTSMAPRLAEIYGTQAYETGVGFKFVGPKMTEMHAVIG
                     GEESGGFGFGMHIPERDGLASGLFLLDLWLTRGKKASELLAELQALAGPSYYNRIDIR
                     FAREGYDTVKADTLARLEHEAPETLAGRPVTRRTALETGDGFKFYRDDGAWLLIRFSG
                     TEALLRVYVEARSPEDVETLLEEGRRIATAGVPV"
     gene            complement(1834..2442)
                     /locus_tag="VIN32_04230"
     CDS             complement(1834..2442)
                     /locus_tag="VIN32_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921821.1"
                     /translation="MAATARTSGPPVIQGQALFAIWHESNLVAATAAYRLRATKRFIS
                     FSTRGFRGIVMNTMLESMGSGVVALPEESNRAEAAKLARELARIGRDGSSLVVSCDGP
                     TGPYRVAKPGVLIVARESGIPIQPWAVAIRPALRLNGRWDRHLVPVPFCRMRVEEGVP
                     IPIAPREPLKPRVAELQAALDGVQGLADRRMAERRRRTIGAS"
     gene            complement(2543..3613)
                     /locus_tag="VIN32_04235"
     CDS             complement(2543..3613)
                     /locus_tag="VIN32_04235"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEY8921822.1"
                     /translation="MFAIVMAGGSGTRLWPLSRRSSPKPLLALTGDTSLLQQTVARLG
                     NLLKPHDIYVITSQAHVRATQAQLPQLPPDNVLGEPLARSTAVAAGLATVMARRQSDE
                     VALVLPADHFVADEGRFIEALQEAARTAERGYLVTLGVVATAPATGYGYVKQGAPIHA
                     GQPTALVERFVEKPSAADARKMLEDGGYLWNAGIFAWRVYAYRQAMEKFQPELAEALD
                     KIAALNRTPGWVSDVREILEPVHAISIDVGIAEPAAADGRMAVVPMDAGWSDIGSWSA
                     LLEALSARSGATLVASGQHLDRGSERVLVHGGERLVVTVGLRDIIIVDTPDALLVCAR
                     DRAEEIKPVLDEIAEKAGDRYL"
     gene            complement(3613..4248)
                     /locus_tag="VIN32_04240"
     CDS             complement(3613..4248)
                     /locus_tag="VIN32_04240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921823.1"
                     /translation="MTPRATPRSPIRWGEPPRGGGLGAAFSALASRVREMLAGRHVPA
                     RQSLPALPLVLRHGELAQRYLAAEGVADHPSAAAAASTAVEQAVAERAWWPADVWGHR
                     ALWHFEQAGMELEATRAARRIGDVRVGGGDPASSRRYYAEAISEARDIGAEREEGLAA
                     LGMGRAELELRNVTTARRLGQIAVDLLERAGAPPDESAAARDLRGEEKEVG"
     gene            complement(4257..>4915)
                     /locus_tag="VIN32_04245"
     CDS             complement(4257..>4915)
                     /locus_tag="VIN32_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methionine adenosyltransferase domain-containing
                     protein"
                     /protein_id="HEY8921824.1"
                     /translation="RRPRRVHTVLVSTQHDPEVAPLELEEGVRELVIRDAIDPELLDE
                     ETRYLVNPTGRFVIGGPMGDSGLSGRKIIVDTYGGMARHGGGSFSGKDPTKVDRSGAY
                     MARYVAKNLVAAGLAQRCELQLAYAIGVAHPLSINLQTFGTGVVADADLAELVERSFD
                     LRPGAIIAGLDLRRPIYRPTAAYGHFGRSDLDLPWERTDRASALREAAGAVGVAGASA
                     "
BASE COUNT          726 a         1739 c         1719 g          731 t
ORIGIN      
        1 ggcccaggcc ggcaggccgt agtcgcggtg caccaccagc ggcacccgga gccgctcacc
       61 ggcgatcccc tcgaccagct ccgcgccgat cgccgagccc cccatcgcca ggacgcagat
      121 gccgtcaacc tcggtgtgcg atgtcggcag cttgaccgcc gcggcgaccc tgcgcgcaag
      181 ggcgagctgt cgcggcagct cgcgaacgcg accgaccatg tcagcggagt cgatcccctt
      241 gatcaccctc gggctgtcga gcgtgatcgc ctcgtcgagc tgtggcggtg cggtcggatc
      301 gtcctcggcc gtctcctcgc gccgtcccgg ctcgtcgtcg ggacgcttgt agccgaagaa
      361 gtcgcgagcg tcgctcatct gctcagaccg ggacgccggc tgtcgcgatg cggcggccct
      421 cctcgaggag cgtctcgacg tcctccggcg atcgcgcctc gacgtagacg cgcagcagcg
      481 cctcggtccc cgagaagcgg atcaggagcc acgcgccgtc gtctcggtag aacttgaacc
      541 cgtcgccggt ctcgagcgcc gtgcgcctgg tgaccgggcg cccggcgagc gtttcaggcg
      601 cctcgtgctc gagccgggcg agggtatcgg ccttgacagt gtcgtagccc tcccgggcga
      661 agcggatgtc gatgcggttg tagtagctcg gcccggccag cgcctggagt tccgccagca
      721 gctcggacgc cttcttgccc cgggtcagcc acaggtcgag caggaagagg ccggacgcca
      781 ggccgtcgcg ctcggggatg tgcatcccaa agccgaatcc gcccgactct tccccgccga
      841 tgacggcatg catctccgtc atcttggggc cgacgaactt gaagccgacc ccggtctcat
      901 atgcctgcgt gccgtagatc tcggccagcc gcggggccat ggaagtgctg gtcaccgtgt
      961 agacagcggg gccgatctgg ccgcgcacct ccagcaggta ccagtacagc agcccgtaga
     1021 cctgcagctg gttgacgaag acgccctcct cggttgccat cccgacccgg tcggcatcgc
     1081 cgtcgaaggc gatcccgatg tcggcgcccc agcgcgggat ctcctcgagc caggcgtcga
     1141 tgttggggcg gatcggctcg gggttgacgc ctccgaagaa cgggttgcgt tcggtgtgca
     1201 gctcgttgac ggtcagtccg gccccgccga gcagccgggt gaaccagccg gcgcccgatc
     1261 cgtacatcgg ctcgaccagg acgcggccct tggcagcccg gatccgctcc aggtcgacga
     1321 tgctggccag ctgctcccgg aagcgcgggt atgggtcgaa ggtcgccacc aggccggcgg
     1381 cgacggcctc ggcgtagtcg cgccgaggcg gaagctcgtc ctcgggatgg cgatccatgg
     1441 tcgcctcgat cgcggccagc atctcgggag cggcggcgcc accggtatcg gccttgacct
     1501 tgaagccgtt gtcggtccat gggttgtggc tggcggtgat cacgatcccg gccccggcag
     1561 ccatctcgcg ggtgaagaag ctgccgacct gggtcgggac cgcgtcgggt gtcatgaacg
     1621 agcgaatgtc atgcgcggcg agcacctcga cgcacgcctg ggcgaaatac tccgatgcga
     1681 atcgacggtc gtggcagacc accacgccgc ggtccgcggt cccctccgcg gccaggtact
     1741 cggccacgcc acgggcgcag cgacgcacgt tgtggaaggt gaagtcctcg gcgatagcgg
     1801 ctcgccagcc gtcggtgccg aacttgatcg tggtcacgag gcccctatcg tacggcgccg
     1861 gcgctccgcc atccggcggt cggcgaggcc ctgcacgcca tccagcgccg cctgaagctc
     1921 agcgacgcgt ggcttgagtg gctcccttgg cgcgatcggg atcggcaccc cctcctcaac
     1981 ccgcatccgg cagaacggaa cgggaacgag gtggcgatcc cagcgtccgt tcaggcgcaa
     2041 tgccggccgg atcgcgacgg cccatggctg gatggggatg cccgactcgc gtgcaacgat
     2101 gaggaccccc ggcttggcga ctcggtaggg tccggttgga ccatcgcagc tgacgaccag
     2161 gctggagccg tcgcggccga ttcgcgccag ctccctggcc agcttcgccg cctccgctcg
     2221 attcgattcc tcgggcagcg caacaacccc cgagcccatc gactcgagca tcgtgttcat
     2281 cacgatccca cggaagccac gcgtgctgaa gctgatgaac cgcttggtgg cccgcagtcg
     2341 gtacgccgct gtggcggcaa ccaggttcga ctcgtgccag atggcgaaga gcgcctggcc
     2401 ctggatgacg ggcggccctg acgttcgcgc ggtggcggcc acgagtcgaa ggtagagcgc
     2461 catggcgcga ccgatcagcc gagcgagcca cgcgagccgg attgagtcct cctgggtggc
     2521 cgccgggacc gggccgatcg gctcagagat agcggtcgcc ggccttctcg gcgatctcgt
     2581 ccaggaccgg cttgatctcc tcggcacggt cccttgcaca gaccagcagc gcatcggggg
     2641 tgtcgacgat gatgatgtcc cgcagaccga cggtcaccac cagccgttct ccgccgtgaa
     2701 ccagcacgcg ttccgatccg cggtcgaggt gctggccgga cgcaacgagg gtcgcgccgc
     2761 tgcgcgcgga gagcgcctcg aggagggcag accatgagcc gatgtcgctc cagccggcgt
     2821 ccatcggcac cacggccatc cgtccgtccg cagccgccgg ctcggcaatc ccgacgtcga
     2881 tcgagatggc gtggaccggc tcgaggatct cgcggacatc ggagacccag ccgggtgtgc
     2941 gattgagcgc cgcgatcttg tcaagcgcct cggcgagctc gggctggaac ttctccatcg
     3001 cctggcggta ggcgtagacc cgccacgcga agatccccgc gttccacagg tagccgccat
     3061 cttcgagcat cttccttgcg tcggcggcgc tcggcttctc gacgaagcgc tcgaccagcg
     3121 cggtcggctg cccggcatgg attggtgcgc cctgcttgac gtacccgtat ccggtggcgg
     3181 gggcggtggc aaccacgccg agggtgacca ggtacccgcg ctccgccgtc cgggcggcct
     3241 cctggagcgc ctcgatgaag cgtccctcgt ccgccacgaa atggtcggcc ggcaggacca
     3301 gcgcaacctc gtcgctctgc ctgcgagcca tgaccgtggc gagcccggct gcgaccgccg
     3361 tcgagcgcgc cagcggctcg cccagcacgt tgtcgggggg caactgcggg agctgcgcct
     3421 gcgtcgcgcg cacgtgcgcc tgggaggtga tgacgtagat gtcgtgcggc ttcagcaggt
     3481 tgccaaggcg cgccacggtc tgttgcagca ggctcgtgtc gccggtgagc gccagcagcg
     3541 gcttggggga ggagcgccgc gaaagtggcc agaggcgggt gccggacccg ccggccatca
     3601 cgatcgcaaa catcaaccga cctctttctc ctcgccgcgc aggtcgcgcg ccgcggcgga
     3661 ctcgtcgggc ggagcgccgg cgcgctccag caggtcgact gcaatctgac cgaggcggcg
     3721 agccgtggtc acgttgcgca gctccagctc ggctctaccc atgccgagcg ccgcgaggcc
     3781 ctcctcccgc tcggcgccga tgtcccgagc ctcgctgatt gcctccgcgt agtatcggcg
     3841 gctgctggct gggtcaccgc cgccaacacg cacgtcgccg attcggcgcg ccgcgcgagt
     3901 ggcctccagc tccatgcctg cctgctcgaa gtgccacaga gcgcggtgcc cccaaacgtc
     3961 ggcgggccac caggcccgtt ccgcgactgc ctgctcaacc gcggtcgagg ccgctgcggc
     4021 cgcgctggga tggtctgcca cgccttcggc ggcaaggtag cgctgggcaa gctcgccatg
     4081 gcgcagcacc aacggaagcg cgggcaggga ctggcgggcg ggaacgtggc gccctgcgag
     4141 catctcccgc acgcgcgaag cgagcgccga gaacgcggcc ccgaggccgc ctccacgcgg
     4201 cggctctccc cagcgaatcg gtgaacgggg cgtggcgcgg ggcgtcaccg cgtcgctcag
     4261 gccgaagccc cagcgactcc caccgcgccg gccgcctcgc gcagggcgga ggcacgatcg
     4321 gtccgctccc agggaaggtc gaggtcgctg cgcccgaagt ggccgtaggc ggcggtgggg
     4381 cggtagatcg gcctgcgaag gtcgaggccg gcgatgatcg cgccgggacg cagatcgaag
     4441 ctgcgttcga ccaattcggc caggtccgca tcggcgacca cgccggtgcc gaaggtctgc
     4501 aggttgatcg acagcggatg ggccaccccg atggcatagg ccagctggag ctcgcaccgc
     4561 tgcgccagac ccgccgcaac caggttcttg gccacgtagc gggccatgta ggcgcccgat
     4621 cggtccacct tggtggggtc cttgccgctg aagctgccgc cgccgtgacg ggccatgccg
     4681 ccgtaggtgt cgacgatgat cttgcgcccg gacaggccgg agtcccccat cgggccaccg
     4741 atcacgaagc gccctgtcgg gttgacgaga tagcgggtct cctcgtcgag gagctccgga
     4801 tcgatcgcgt cacggatcac cagctcgcgg acaccctcct cgagctcgag cggggcaacc
     4861 tcgggatcgt gctgcgtgct gaccaggacg gtatggaccc ggcgcgggcg tcgat
//
LOCUS       DATCQR010000127        10459 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_69792, whole genome shotgun sequence.
ACCESSION   DATCQR010000127 DATCQR010000000
VERSION     DATCQR010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 10459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10459
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69792"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(65..562)
                     /locus_tag="VIN32_04250"
     CDS             complement(65..562)
                     /locus_tag="VIN32_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018838437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HEY8921825.1"
                     /translation="MGDDLGQQHPNLARVIAAAASAGLPIEVQRFPGGTRTAEDAARA
                     VGCDVAQIVKSLVFVADGEPVIALVSGADRLDPMRLAAATGASAVRRADGDEARAATG
                     FAIGGVPPIGHARPLTVLMDERLLAHDLVWAAAGLPDAVFKASPTALQAAAGARVVAL
                     AATRE"
     regulatory      605..707
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            697..1347
                     /locus_tag="VIN32_04255"
     CDS             697..1347
                     /locus_tag="VIN32_04255"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HEY8921826.1"
                     /translation="MKAIVVAGGNAASEDAAQLAGADMVIAADSGAQWLDGRGLSPDL
                     VIGDMDSIDPALLERLAERGVEIEEHPAEKDASDVELAVSWALAGGANEVVIVGGLGG
                     GRLDHELANLLLLVDRRWDGIRLRLVRDGTTARALHAGRRRELDGVPGDLVTLLPVGA
                     DATGVITEGLRYPLAGETLQSGRSRGLSNEVEHAPASVSLEGGTLLIIETRKESSV"
     gene            1344..1931
                     /locus_tag="VIN32_04260"
     CDS             1344..1931
                     /locus_tag="VIN32_04260"
                     /inference="COORDINATES: protein motif:HMM:NF021348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEY8921827.1"
                     /translation="MTVMSNSRVGPWRTVDVVTAAVLAVAFGAVFQAWNVLWEATTWV
                     LPPVRGIVYGTWMLPAVVVPLILRRPGAALLAETMAAAAEVLFGAPWGLVLVVYGLTQ
                     GAAAELIFAFGLYRRWGLIAAIGAGAAAGVAGALLDLAFYYPEWGTDWMVLYTALVAG
                     SSALIAGLGGWLLVRALGRTGVLSAFPAAREQPEV"
     gene            1954..3354
                     /locus_tag="VIN32_04265"
     CDS             1954..3354
                     /locus_tag="VIN32_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018216592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY8921828.1"
                     /translation="MEARGWGWRHTGRRAWALRGLDLRIEAGERVMLLGSSGAGKSTL
                     LSGLAGLLEGGESEGVLRVDGLDPREARERTGIVFQDPESSLVMARAGDEVAFGLENR
                     CVPAGEIWPRVDQALEMVGFPYGRDRPTDALSGGEQQRLALAAALALRPELLLLDEPT
                     ANLDPAGAALVRDAIGRLAAGGQLTMLLVEHRVADASDLVDRVIVLEAGGGVVADGPP
                     SGVFATRGDELAAAGIWVPNKPLPMPAERGRPEPEAVLVAEQVGYRFPGEQSLAVGPM
                     DVAVRSSEALAIVGPNGSGKSTLALLLGGLLRPSTGSVIGPDQKPICRLPAHRLAQFI
                     GSVFQDPEHQFLMGTVREELLLGPLQKHASAAEASRRAWEMMERLHLAALAEANPFSL
                     SGGEKRRLSVATALVAEPSVLILDEPTFGQDRRTAVELLDLLAQQRDEGTAVCFVTHD
                     LPFVAALADRTVELAP"
     gene            3351..4100
                     /locus_tag="VIN32_04270"
     CDS             3351..4100
                     /locus_tag="VIN32_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018823818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HEY8921829.1"
                     /translation="MRRFTPLRPDPRAPLARANPVAKLGTAAILMAVLFLASGPIAPA
                     VILAGIVASLPLTGLGVRTLLVRTWPLLIAALVVGVLNVVLAPAAPGGPDVATGVALG
                     LRLLGIALSGVVALATSDPTDLADSLQQQARLSPRLAVGALAAIRMLPILAVEWQLLG
                     MARRARGISGGWSPVAAAQLAFGKLLALLVGAIRRATRLALAMDARGFGTAACRTIAR
                     PQRMRVADWGLLLAAVALGAVALGIGSALRV"
     gene            4121..5098
                     /locus_tag="VIN32_04275"
     CDS             4121..5098
                     /locus_tag="VIN32_04275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY8921830.1"
                     /translation="MPRSEELRVRAGDASLAATLLLPDDPAPNGANGRYPNVLLLASW
                     LPRDRDGAYDRVGHPGWFAPAAPVTPPGLLARIAEALAIRGVASLRSDPRGCGASEGR
                     WEEVTLFAKIDDARDMLAVMRGRRELDLRRSGIIGHGEEAGIGLAVAIGDPALSALTL
                     IGPSARSWRETLRRAAATRGREGTEHQHPIVAAIDRWSEELIEHAERREPTLTLPLHG
                     AGLVTLALAPFEQAIHTPPLALATMLHRSIALVHGEADSWSDPDESALLAAALSDAGN
                     EPGSRLIPDAGHDLAEASGEVVGEIAGDLADRILPRDLPPVLLAIEEMG"
     gene            5113..5577
                     /gene="bcp"
                     /locus_tag="VIN32_04280"
     CDS             5113..5577
                     /gene="bcp"
                     /locus_tag="VIN32_04280"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010668248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HEY8921831.1"
                     /translation="MTQPSVGDPVPPFEMQADDGSTVSSQGLLAQRYVVYFYPKDDTP
                     GCTTQACSLRDNYERVTAAGVEVYGVSPDSVASHVKFREKYGLPYRLLSDEGHRAADA
                     FGTWVEKKFAGKTYKGVERTSFIIGPDGRIEHVLPRVKAADHVDLLMERLAA"
     gene            5574..5720
                     /locus_tag="VIN32_04285"
     CDS             5574..5720
                     /locus_tag="VIN32_04285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921832.1"
                     /translation="MIWIGIAVTLVAWFIGLLLQLGPLVNLLLVAAAVLLVVQVINER
                     QEEP"
     gene            5717..5893
                     /locus_tag="VIN32_04290"
     CDS             5717..5893
                     /locus_tag="VIN32_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921833.1"
                     /translation="MSPAWRYLAWRSLPVVLMLAWFLLLIGGFGLGGLLHLFLLAGIA
                     VFAYQLRADRSTQA"
     gene            5950..7041
                     /locus_tag="VIN32_04295"
     CDS             5950..7041
                     /locus_tag="VIN32_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018354678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEY8921834.1"
                     /translation="MRILHFADLHLDAPFAWARPETARLRRRNRRETLTRILQLADDE
                     RVDAILCAGDLFEHERFTPDTVAFLRESFARTDRPIYLAPGNHDWYSERSPYALEEWS
                     PNVHLFTDARLTPVELADGVTLWGAAHRAPANTGPFFADFRPDRGGVHLAVAHASERS
                     ALPFQEPDKELHAPFDAAELEAAGIDHAFLGHYHVARDRDRYTYPGNPDPLAFGESDG
                     RGVVLATVAADGTVTRERRVVAVSQVYEVPVALDGCLHVDDLRDRVSQAIAGLSGSVR
                     VTLNGEVPADLPIEPRALSALGDHLEGLVVRTGAIVPAYDLEAIKAEMTVRGQFARDA
                     LLIADEDRQRKVLITGLRALEGRRDLEVA"
     gene            7046..9595
                     /locus_tag="VIN32_04300"
     CDS             7046..9595
                     /locus_tag="VIN32_04300"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY8921835.1"
                     /translation="MWIERVIARAFGPLRDETLHLGEGMNVIGGPNEAGKTSWHAATR
                     LALTGVRRGPGRTKEVASVIDRHRPWDSPEHWEVEARLHLGDGRIVDISQDLAGKVAC
                     RARDVTLGDDLSNEIINDGTPDATRWLGLDRESFSAVVAVSQAQIMAVAESASGLQDQ
                     MQRAAATRGTDATAAEAIERLKGFRKESVGAVRVGATGPLWAAMREQEAADAALLDAR
                     RLHGEYLDRESRLEAAAREHQCAERDLTVAEAALAVREATQLSERALRAAELASSYPA
                     APARLATRDALADEVAAALDSWRRRPAPVPLNGSSASELEQQLAAIPAVPEGDRRPDR
                     SVADAARALDLAAEAMRQHLQRSPSTTLPGVVQAAPASHYRWGLPIVGAAVAAIVSVV
                     LLATSQALAGIVLLGVAGAMGAWIVRGRTASGSAPDERASAELSARAATEWEAEATRL
                     RSAAQEANERLRAALVARGAAAGEDLRAAVAAYELECAGREDMARAAAGADGLRRAIE
                     ARHAAEDAAHRVESAVSDAESAIRLAVEHIGEAGDGDTASAVERLKAWQRTRAEVAQA
                     GETAIREWQELQGLLAGGTLAELQAEAERRAQVAARLARRVGDEAVIPLAGDSEAALA
                     TARAEAARSKAVVDELGGELRLLATTLANVAEAEERADAARVELGRVQNLAAVIDETL
                     TLLEAAERRVHRDIAPILTAAVQRHLPAITGGAYVEVGMNPRDLSVDVKEARTGQWRD
                     ARLLSEGTREQIYLLLRVAMAEHLVTTAESAPLLLDEVTAQSDPERKLRMLDVLHALS
                     ADRQIVLFSHDAEVMDWAALALREPRDRLIRLGGSDASHQVPGPLAEAAIG"
     gene            9656..10375
                     /locus_tag="VIN32_04305"
     CDS             9656..10375
                     /locus_tag="VIN32_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HEY8921836.1"
                     /translation="MPNDRLPTPERVLVVAAHPDDIEFGAAGTVARWIGEGAVVQYLM
                     MTRGDKGSDNPAADPAALASLREGEQRAAAAEIGVTEVEFLGEPDGQVEPSLRLRERV
                     TRAIRAFQPEIVMSHDPTVLFVNNEWINHPDHRAVGTVVVDAVFPTARDPLNFPEHLA
                     AGLAAWKVAELFLWSTNEANQLVDIGDTLERKIAALAHHASQFRDFGETARWLRRGAE
                     ELGERAGYPAAEGFRRVMLAR"
BASE COUNT         1453 a         3656 c         3790 g         1560 t
ORIGIN      
        1 gaacggcgga cggccgggtg gcgatcgaga tcaggctgga tcgaccgtga caataaccgt
       61 cgagtcactc tcgagtcgcc gccagcgcca cgacgcgtgc gcccgcggcc gcctgaagcg
      121 ccgtggggga cgccttgaat acggcgtccg gcaggccggc tgcggcccac accagatcgt
      181 gggccaggag tcgctcgtcc atcaggacgg tcagcggacg agcatggccg atgggcggca
      241 ccccgccgat ggcgaacccg gtcgccgccc gggcctcgtc gccatccgcg cggcggacgg
      301 cgctggcacc cgtggctgcc gcgagtcgca tcggatcgag ccggtcggcc ccagacacca
      361 gggcgatcac cggctcgccg tcggccacga acaccagcga cttgacgatc tgggcgacgt
      421 cgcagcccac ggcccgcgcc gcgtcttccg ccgtccgcgt ccccccgggg aatcgctgga
      481 cctcgatcgg caggcccgcg ctcgccgcgg cggcaatgac gcgcgcaagg tttggatgct
      541 gctgacctag atcgtcaccc acggcccgat gatcgcacga gtgctacgct caccgggaca
      601 accccaagag cgacagggga gcgcgaaagc gctgagagtg cggaaggccg cagaccctcg
      661 aacctgatct gggtcatgcc agcgaaggga gtcggagtga aagcaatcgt ggtcgccggc
      721 ggcaatgccg cgtcggaaga cgctgcccag ctggcgggcg ccgacatggt gatcgccgcc
      781 gactccggtg cccagtggct ggacggccgc ggcctgtctc cagacctcgt gatcggcgac
      841 atggattcga tcgaccccgc cctcctcgag cgcctcgccg aacgcggagt cgagatcgag
      901 gagcatcctg ccgaaaagga cgcttcggat gtcgagctcg ccgtgtcgtg ggcgctcgcc
      961 ggcggcgcca acgaggtggt gatcgtgggg ggcctcggcg gggggcggct ggatcacgag
     1021 ctggcgaacc tgctcctgct cgtcgaccgc cgatgggatg gcatccgcct ccgcctcgtg
     1081 cgcgatggga cgaccgcgcg cgcactccac gccggccgcc gccgcgagct cgatggagtc
     1141 ccgggtgacc tggtgacact gctccctgtc ggggcggacg cgacgggagt gataacggaa
     1201 ggactgcgct acccgctcgc cggggagacc ctccaatcgg gtcgctcgag gggtctctcc
     1261 aacgaggttg agcacgcccc ggcatcggtc tcgctggaag gcgggacgct gctgatcatc
     1321 gaaacacgaa aggagtcgag cgtatgacgg tcatgagcaa ctctcgcgtt gggccgtggc
     1381 ggaccgtcga cgtcgtgacg gcggcagtcc tggccgtggc attcggagct gtctttcaag
     1441 cctggaatgt gctctgggaa gcgaccacat gggtgcttcc cccggtgcga ggaatcgtct
     1501 acgggacgtg gatgctgcct gccgtggtcg tcccccttat cctccgccgg ccgggggccg
     1561 cgttgctcgc cgagaccatg gctgcggcag cggaggtgct attcggggcg ccgtggggac
     1621 tggtgctggt ggtttacggc ctcacgcagg gggcggcggc tgagctcatc ttcgcctttg
     1681 gcctgtaccg gcgttggggg ctcatcgccg ccatcggagc aggcgcggct gctggagtgg
     1741 ctggcgcgtt gctcgacttg gccttctatt acccggaatg gggcacggac tggatggtgc
     1801 tgtacacggc gctcgtcgct ggcagctcgg ccctcattgc cggcctcggc ggctggctcc
     1861 tggtccgcgc cctcggccgc acgggcgtcc tgagcgcctt cccggcggcc cgcgagcagc
     1921 cggaggtctg agccactgag cgcgccggtc gcgatcgagg cgcgcggctg ggggtggcgg
     1981 cacacgggtc gtcgtgcgtg ggcgctgcgc ggcctcgacc tacgaatcga ggccggggag
     2041 cgcgtcatgc tgctcggctc ctcgggtgcc gggaagtcga ccctgctttc cggcctggcg
     2101 ggtctgctgg agggcggtga gtccgagggt gtcctgcggg tcgacgggct cgatccgcgg
     2161 gaagcccgcg aacggaccgg gatcgtcttc caggatcccg agtcgagcct cgtgatggcg
     2221 cgtgccggcg acgaggtcgc gtttggcctg gagaaccgat gcgttccggc gggcgagatc
     2281 tggccgcggg tcgaccaggc actcgagatg gtcggcttcc cgtatggccg ggatcgcccc
     2341 accgatgccc tctcaggcgg tgagcagcaa cggctggcgc tggcggcggc gctggcgttg
     2401 cgccccgagc tcctgctcct cgacgaaccg accgccaacc tcgacccggc gggagccgcg
     2461 ttggtgcgcg acgcgatcgg ccggctggcg gccggcgggc agctgaccat gctgctggtc
     2521 gagcaccgcg tggctgatgc atcggatctt gtcgaccgcg tgatcgtgct ggaggctggt
     2581 ggcggggtgg tcgccgatgg gccaccgtcc ggggtgttcg ccacccgcgg cgacgagctc
     2641 gcggcagccg ggatctgggt gccgaacaag ccactgccga tgccggccga gcgcggacgc
     2701 cccgagcccg aggcggtgct ggtggcggag caggtcggat accgcttccc cggggagcag
     2761 tcactggccg tcggaccgat ggatgtcgcg gtccggtcct ccgaggcgct ggcaatcgtc
     2821 gggccgaatg gatccggcaa gtccacgctg gccctccttc tgggtggtct cctccgtccg
     2881 agcacggggt cggtgatcgg tcccgatcag aagccgatct gccggctgcc ggcccatcgg
     2941 ctcgcgcagt tcatcggctc cgtcttccag gaccctgagc accagttcct gatgggaacc
     3001 gtccgcgagg agctgctgct cggcccgctc cagaagcatg cttccgcggc ggaggcgtcg
     3061 cggagggcat gggagatgat ggagcgactg cacctcgcgg cactcgcgga ggccaatccg
     3121 ttcagcctct ccggcggcga gaagcgccgc ctctcggtcg ccaccgccct ggtcgcggag
     3181 ccgagcgtcc tgatcctcga cgagccgacc ttcggccagg accggaggac ggcggtcgag
     3241 ctcctcgacc tgctcgcgca gcagcgtgac gaggggaccg cggtctgctt cgtgacgcat
     3301 gacctgccgt tcgtcgccgc gctcgccgac cgcacggtgg agctggcccc atgaggcgct
     3361 tcacaccgct tcggcccgac ccgcgcgcac cgctggcccg cgccaacccg gtggccaagc
     3421 tgggcaccgc cgcgatcctg atggccgtcc tcttcctggc gtcgggaccg atcgccccgg
     3481 cggtcatcct ggcggggatc gtggccagcc tgccgctcac cggtctcggg gtgcgcacgc
     3541 tgctggtgcg gacatggccg ctgctgatcg ccgccctcgt ggtcggcgtg ctcaacgtgg
     3601 tgcttgcgcc cgctgcgccg ggtggcccgg acgtcgccac gggcgtcgcc ctggggctgc
     3661 gcctgctggg gatcgcgctc tccggggtcg tcgccctggc cacctccgac ccgacagacc
     3721 tggccgattc gctccagcag caggctcggc tctccccgcg actggcggtc ggggcgctgg
     3781 ccgcgattcg catgctgccg atcctcgcgg tcgaatggca gctgctgggc atggcgcggc
     3841 gagcgcgcgg catcagtggc gggtggtccc ccgtcgccgc cgcgcaactc gccttcggca
     3901 agctgctggc cctgctggtt ggtgcgattc ggcgcgcaac ccggcttgcg ctggcgatgg
     3961 acgcgcgcgg cttcggaacc gcggcctgcc gcacgatcgc acggccgcaa cgcatgcggg
     4021 tcgccgactg gggactgctc ctggcagccg tcgcactcgg tgcggtcgcg ctcgggatcg
     4081 ggtcggcgct ccgggtctag ggcgcgtgta ccctcgtggc atgcctcgct ccgaggagct
     4141 ccgtgttcga gctggcgacg cctccctggc ggcgacgctg ctgctgccgg atgacccggc
     4201 gccgaatggg gcgaatggcc gctacccgaa tgtgctgctg ctcgcctcct ggctgccgcg
     4261 cgaccgcgac ggcgcctacg accgggtcgg ccacccaggc tggttcgcgc ctgccgcgcc
     4321 agtgacgccg cccggcctgc tcgcccgcat tgccgaggcg ctcgccattc gcggggtggc
     4381 ttcgctgcgc agcgacccgc gtggatgcgg cgcctccgag ggtcggtggg aagaggtcac
     4441 cctcttcgcc aagatcgacg acgcccgcga catgctcgcc gtcatgcgcg gccgcaggga
     4501 gctcgacctg cgccgatccg ggatcattgg acacggcgag gaggcgggga ttggcctggc
     4561 cgtcgcgatc ggggatcccg ccttatcggc tcttaccctg atcgggccgt cggcgagatc
     4621 gtggcgcgag acgctgcgtc gcgcggccgc aactcggggg cgcgagggca cggagcatca
     4681 gcacccgatc gtggccgcca tcgaccgctg gagcgaggag ctgatcgagc atgccgagcg
     4741 acgagagccg actctcacgc tgccgctcca tggagccggg ctcgtgacgc tggccctggc
     4801 cccgttcgag caggcgatcc acacgcctcc gcttgcgctc gccacgatgc tgcatcggtc
     4861 catcgcgctc gttcacggcg aggccgacag ctggtccgat ccggacgaat ccgccctcct
     4921 cgcagccgcc ctctccgacg ccggcaatga accggggagc cggctcatcc cggacgccgg
     4981 tcacgacctg gcggaggcga gtggcgaggt cgtgggcgag atcgcagggg acctggccga
     5041 tcgcatcctt ccccgcgacc tgcccccggt cctgctggcg atcgaggaga tggggtgagg
     5101 tacgattcga acatgaccca gcccagcgtg ggagacccgg tcccgccctt cgagatgcag
     5161 gctgacgatg gctcgacggt ctcgagccag ggcctactgg cgcagcgcta cgtcgtctac
     5221 ttctacccca aggacgacac ccccggctgc acgacccagg cctgctcgtt gcgcgacaac
     5281 tacgagcgtg tgaccgccgc cggggtcgag gtctacggcg tctcgcccga ctcggtggcg
     5341 agccacgtca agttccgcga gaagtacggc cttccgtacc ggctcctctc cgacgaggga
     5401 cacagggccg ccgatgcgtt cggcacatgg gtcgagaaga agtttgccgg gaagacttac
     5461 aagggcgtgg agcggacctc cttcatcatc ggcccggatg ggcgaatcga gcacgtcctg
     5521 ccgcgcgtca aggcggccga ccacgtggac ctgctgatgg agcggctggc ggcatgatct
     5581 ggattgggat cgcagtgacg ctggtggcct ggttcatcgg gctgctgctg cagctggggc
     5641 ccctggtcaa cctgctgctg gtcgcggcgg cggtgctgct tgtcgtccag gtcatcaacg
     5701 agcgccagga agagccgtga gccccgcctg gcgatacctg gcctggcgct cgctgcccgt
     5761 cgtgctgatg ctcgcctggt tcctgctcct aatcggcggc tttggccttg gcggcctcct
     5821 gcacctcttc ctgctggcgg ggatcgccgt cttcgcgtac cagctccggg cggaccgctc
     5881 gacccaggcg tgactcggtg tccaaacgcg acggcctagc tgtcgcgaac ggggctaggc
     5941 ttggcgatca tgcggatcct gcatttcgcc gacctgcacc tcgacgcgcc attcgcctgg
     6001 gcgcggcccg agaccgcgcg cctgcggcgg cgcaaccggc gagagaccct cacccgcatc
     6061 ctccagctgg ccgacgacga acgtgtcgac gccatcctgt gcgcgggcga cctctttgag
     6121 cacgagcggt tcacgccaga cacggtcgcc tttctgcgcg agagctttgc acggaccgat
     6181 cggccgatct acctcgctcc cggcaaccac gactggtact ccgagcgcag cccgtatgcg
     6241 ctcgaggagt ggagcccgaa cgtccacctg ttcaccgatg cccggctgac gccggtcgag
     6301 cttgccgacg gcgtcaccct gtggggcgcc gctcaccgcg cgcccgccaa tacggggccg
     6361 ttcttcgccg acttccgccc cgatcgaggg ggcgtgcacc tggccgtcgc ccacgcatcc
     6421 gagcgcagcg ccctgccatt ccaagaaccc gacaaggagc tgcacgcccc gttcgacgcg
     6481 gcggagctcg aggcggccgg gatcgaccac gcattcctgg gtcattacca cgtcgcccgc
     6541 gaccgcgacc gctacaccta ccccggcaat ccggatccgc tcgcattcgg cgagtccgac
     6601 ggacgcgggg tcgtcctcgc cacggtggcc gcagacggga ccgtgacccg cgaacggcga
     6661 gtcgtcgcgg tgagccaggt gtacgaggtg cccgtcgcgc tcgacggatg tctccatgtc
     6721 gacgacctca gagatcgggt cagccaggca atcgccggcc tctccggctc ggtgcgggtc
     6781 acgctcaacg gcgaggtacc cgcggacctg ccgatcgagc cgcgagcgct atccgccctc
     6841 ggtgaccacc tcgaaggcct ggtggtgcgg accggtgcca tcgtgccggc gtacgacctc
     6901 gaggcgatca aggccgagat gacggtgcgc gggcagttcg cgcgcgacgc gctgctgatc
     6961 gccgacgagg accggcagcg caaggtgctg atcaccgggc tgcgcgcgct cgaaggtcgc
     7021 cgcgacctcg aggtggcgta gggccatgtg gatcgagcgc gtgattgcgc gagccttcgg
     7081 cccgctgcgt gacgagacgc tgcatctggg cgaggggatg aacgtgattg gcggccccaa
     7141 cgaggccggc aagaccagct ggcatgcggc cacgcgcctg gctctgacag gagttcgccg
     7201 tggcccgggt cgcaccaagg aggtcgcttc ggtgatcgac cgccaccgac cgtgggactc
     7261 gccggagcac tgggaggtcg aggctcgact gcacctagga gacggccgca tcgtggacat
     7321 cagccaggac ctggcgggca aggtggcgtg tcgcgcgcgc gacgtgacgc tcggcgacga
     7381 cctttcgaac gagatcatca acgacgggac gccggatgcg acccgttggc tcgggctcga
     7441 ccgcgagtcc ttctccgcgg ttgtggccgt gagccaggcc cagatcatgg ccgtcgccga
     7501 gagcgcgtcc ggcttgcagg accaaatgca gcgggcggcg gcgacgcgtg gcacggatgc
     7561 caccgcggcg gaggcgatcg agcgactgaa ggggttccgc aaggagtctg tgggcgcggt
     7621 ccgggtcggc gccaccggcc cgctgtgggc ggccatgcga gagcaggagg cggcggatgc
     7681 cgcgctgctt gacgcacgac gcctgcacgg cgagtacctc gaccgcgagt cgcgactaga
     7741 agcggccgcg cgtgaacacc agtgtgccga gcgcgacctc accgttgccg aggccgcgct
     7801 ggccgtgcgc gaggccaccc agctctccga gcgtgcgctg cgggcagcag agctcgcgtc
     7861 gagctacccg gcagctccgg ccaggctggc aacgcgggac gcgctggctg acgaggtggc
     7921 ggccgccctc gactcgtggc gaaggcgtcc tgcgccggtc ccgctgaacg gttcatcggc
     7981 gagcgagctg gagcagcagc tggctgcgat ccccgcagta ccggagggag accgtcgccc
     8041 ggatcgctcg gttgccgacg ctgcccgggc actcgacctc gcggctgagg cgatgcgcca
     8101 acacctgcag cgctcgccct cgacgacgct gccaggggtc gtccaggcgg ccccggcctc
     8161 tcactatcgg tggggtctgc cgatcgtcgg cgcagctgtt gccgcgatcg tgagcgtcgt
     8221 gctcctggcg accagccagg cgcttgccgg gatcgtcctc ctcggcgttg caggagcgat
     8281 gggggcgtgg atcgtacgtg gtcgaaccgc atccggatcg gcgccggacg agcgagcatc
     8341 cgccgagctg agcgcccgcg cggcgaccga gtgggaggcc gaggcgaccc gcctgcgcag
     8401 cgccgcccag gaagcgaatg agcggctgcg cgccgcgctg gtcgctcgtg gcgccgcggc
     8461 gggtgaggac ctgcgcgccg cagttgcggc atacgagctc gagtgcgccg ggcgcgagga
     8521 catggctcgc gcggcggccg gcgccgatgg ccttcgacgt gccatcgagg cgcgtcacgc
     8581 ggcggaggac gccgctcacc gggtggagtc ggccgtttcc gatgccgaga gcgccattcg
     8641 gctcgccgtc gagcacatcg gtgaggccgg ggatggcgac accgcatccg cggtcgagcg
     8701 cctcaaggcc tggcagcgaa cccgtgcaga ggtggcacag gctggcgaga ccgcaatccg
     8761 cgagtggcag gagctccagg gcctcctcgc cggcgggacg ttggcggagc tccaggccga
     8821 ggccgagcga cgggcgcagg tcgccgccag acttgcgcga cgggtcggcg acgaggccgt
     8881 catccccctc gccggcgact ctgaagcggc tttggccacc gcgcgcgccg aggctgcgcg
     8941 ttcgaaggcg gtcgtcgacg agctgggcgg tgagctgcgc ctgctggcga cgaccctcgc
     9001 gaacgtggcc gaggccgaag agcgcgccga cgcggcccgt gtcgagcttg gtcgcgtcca
     9061 gaacctggcc gcggtgatcg acgagaccct gaccctgctc gaagccgctg agcggcgcgt
     9121 gcatcgcgat atcgccccga tcctcaccgc cgccgtgcag cggcatctgc cggcgatcac
     9181 agggggcgcc tatgtcgagg tgggcatgaa cccgcgggac ctgtccgtcg acgtcaagga
     9241 ggctcgcacc gggcagtggc gcgatgcccg actcctctcg gagggaacgc gcgagcagat
     9301 ctacctcctg ctgcgggtcg ccatggccga acacctcgtc accaccgccg agtcggcgcc
     9361 cctcctgctc gacgaggtga cggcccaatc ggatcccgag cgcaagctcc gcatgctcga
     9421 cgtgctccac gcgctcagcg cggaccgcca gatcgtcctc ttcagccacg acgccgaggt
     9481 gatggactgg gccgcgctgg cgctccgcga gccgcgcgat cggctcatcc ggctgggcgg
     9541 ctcggatgcg tctcaccagg tcccaggccc actcgccgag gcggcgatcg gctgatcggg
     9601 gactcggctg ctacgattcc ggggcctcca actggtcccg ctttactggg agccgatgcc
     9661 caacgaccgc ctccccaccc cggaacgcgt cctggtcgtc gccgcgcatc ccgacgacat
     9721 cgagttcggg gcggcgggga cggtggcacg ctggatcggc gagggcgctg tggtgcagta
     9781 cctgatgatg acccggggcg acaaggggag tgacaacccg gccgcggacc cggccgcgct
     9841 ggcgagcctg cgcgagggtg agcagcgagc cgccgccgcc gagatcggcg tcacggaagt
     9901 cgagttcctg ggcgagccgg acgggcaggt ggagcccagc ctgcgcctcc gcgagcgggt
     9961 cacccgcgcg atccgggcct ttcagcctga gatcgtcatg agccacgacc cgaccgtcct
    10021 gttcgtgaac aacgagtgga tcaaccatcc cgaccatcgg gccgtcggga cggtggtggt
    10081 ggatgccgtc tttccgaccg cacgcgaccc gctcaacttc ccggagcacc tcgcggcggg
    10141 ccttgccgcc tggaaggttg ccgagctctt cctgtggagc accaacgaag ccaaccagct
    10201 ggtcgacatc ggcgacacgc tcgaacgcaa gatcgccgcg ttggcgcatc acgcaagcca
    10261 gttccgcgac ttcggcgaga cggctcgatg gctgcgccgg ggggccgagg agctggggga
    10321 gcgcgccggc taccccgccg cggagggctt tcgccgggtg atgctcgccc gatgatccgg
    10381 tcgcgcgtcg tgacgcgcct cgccacgctc ctgctcgtcg cgtcgctcct ggccgcgtgc
    10441 gcaccagcga cgccggagc
//
LOCUS       DATCQR010000128         7270 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_162495, whole genome shotgun sequence.
ACCESSION   DATCQR010000128 DATCQR010000000
VERSION     DATCQR010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 7270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7270
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162495"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..417)
                     /locus_tag="VIN32_04310"
     CDS             complement(<1..417)
                     /locus_tag="VIN32_04310"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HEY8921837.1"
                     /translation="MRLSLLRWLLVPLVVLPVGWLLFTGLGHDPRLIPSPLVGKPMPA
                     FAGTTLDGGHFSSADLAGKPAIVNFWASYCIPSCVDEHPVLLDLAERHGGELQMVGVL
                     VNDTTDGARGFLARYGQVSWPTILNADGGISIDYGVT"
     gene            complement(421..2403)
                     /locus_tag="VIN32_04315"
     CDS             complement(421..2403)
                     /locus_tag="VIN32_04315"
                     /inference="COORDINATES: protein motif:HMM:NF027651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HEY8921838.1"
                     /translation="MIPTLGHLAVVIGLAATVYAAGAFVIGGRGGDTAVLASARLAMY
                     AAFGLVAFACLAMIVSLLTHDFSVLYVARNNATTTPPFYSVISLWAALEGSILFWTLL
                     ATGWASLVLYRYRSRLPALMPWVGATLALGTAFFFAVMAWPSDPFVRVTPIGLEGNGP
                     NALLQNHPFMGLHPPLLYLGYTGMAIPFAFGMAALITRRTDEEWLAVVRRWTLVPWIF
                     LTAGIVAGAWWSYAVLGWGGYWAWDPVENAALLPWLTATAFIHSSMVAERRGTLRTWT
                     HALVIATFVLTLVGTFLTRSGVVNSVHAFTTSGIGAWFASAILLTLGVALGLLIWRLP
                     DLRDRRPPGAVVSRESAFLFNNVLFLGLTFAVLFGTLYPILVEAMSGARASVGAPWFN
                     RVNAPIFVALLFLTGVGPALPWGGASWATVRERFAIPILSGVGVVIAAWALGVRGAAP
                     LGILGLATFVLVVMGDEVVRGARSRARSRGESAPLAVLRLATRNRRRYGGYLAHVGIC
                     VMAVAIAVSATLGTDRTATLREGESLAIGPYTLTYDRLVTEPLASDPRVIETRAELTL
                     AGPQSGAIATALRDYPNSSTAIATPVVRTALGEDLYVTLLGSDPTTRTATLRVFINPL
                     VMWIWTGGAIVALGALFAIWPDRRRIAVEAALATGA"
     gene            complement(2400..2861)
                     /locus_tag="VIN32_04320"
     CDS             complement(2400..2861)
                     /locus_tag="VIN32_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013457265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HEY8921839.1"
                     /translation="MTLARPVLAPLPPRGRRWGLLVGVAVVVAVIAYLAFSSVGNALV
                     YYLTPTELLARGPAEVGKTERLGGLVKAGTISGEAPDLAFVLTDGTTEISVHAAAAPT
                     RSFREGIGAVVEGELRADGVFEASQVIVKHDENYVAPSQGGLPTQGFDPGS"
     gene            complement(2858..2998)
                     /locus_tag="VIN32_04325"
     CDS             complement(2858..2998)
                     /locus_tag="VIN32_04325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921840.1"
                     /translation="MMNAGFVIAAYGVILGGIGLYGALLARRLRIARDATRRTDDVDA
                     SR"
     gene            complement(2995..3732)
                     /gene="ccsA"
                     /locus_tag="VIN32_04330"
     CDS             complement(2995..3732)
                     /gene="ccsA"
                     /locus_tag="VIN32_04330"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HEY8921841.1"
                     /translation="MKTRILGFVALAAVIGAAINGLFVVPADLNQGDAQRIMYVHVAS
                     AWIAYASFGVTALAGLGYLIWRDLRLDALALAGAEVGVMFTAFAIWGGMMWGRPVWGV
                     YWQWEDPRLTTTALLLALYVGYLLLRRLTDDPARRAARAAVVGLIAAVDIPIVHFSVL
                     WWRGLHQGPTIGDPSKILNPSAPLQFVLALLAMLAAFTLVWVYLLIRRYELARLDWHR
                     EEDARRGFVLAAREAAPGTAAVEGPAR"
     gene            complement(3732..4421)
                     /locus_tag="VIN32_04335"
     CDS             complement(3732..4421)
                     /locus_tag="VIN32_04335"
                     /inference="COORDINATES: protein motif:HMM:NF015344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HEY8921842.1"
                     /translation="MNDLRRGMAVAVAIARKDAISELRGRQAAGSTLFFAALVLLLFG
                     FALGPDSRRLADAAPGLLWLAIVFGGLLTMARLHQLEADDGAFEQLALYPVDRRAIYA
                     GKALAGAAAMLVLGGLLLPVVAVLYAIPIGAAWLPLLVAVALGAIGFAAIGTAYAGLT
                     VRMRAREVMLPLLMLPVVAPLLLAAVKATGAAISGDPLGELGSWLQLLVAFDVIMLVV
                     GAGTYGFLLED"
     gene            complement(4418..5290)
                     /gene="ccmA"
                     /locus_tag="VIN32_04340"
     CDS             complement(4418..5290)
                     /gene="ccmA"
                     /locus_tag="VIN32_04340"
                     /EC_number="7.6.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013614748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015439 - ABC-type heme transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC exporter ATP-binding protein CcmA"
                     /protein_id="HEY8921843.1"
                     /translation="MIAYFGAVARVDAHYPGPHTQPPSIIRPPSSMPQTTIYVAPRLA
                     GTLGTIPPAVATVELARLFGRSAALAGVSLTVEMGRTVALLGPNGAGKTTLLRILATA
                     IRPSYGSVSIDGIDALREAEQVRPRVVYLSHATAHYDDLTAEENIRFAATMFGWGERE
                     GHEVVGEALATVALDAVAAQRVRTFSAGMRKRLALARVLVARPSLLLLDEPHAALDGD
                     GMALVDRLIGLWKEAGVTVMVASHQAERVASLADATMRLEGGLLAEVSGAGVSAGPVG
                     GSPRPRMAVAGMDR"
     gene            5296..6177
                     /locus_tag="VIN32_04345"
     CDS             5296..6177
                     /locus_tag="VIN32_04345"
                     /inference="COORDINATES: protein motif:HMM:NF021214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HEY8921844.1"
                     /translation="MLRAPSLGIILSTWSFDPLPWIGIVLAAALYVAAVRIVDRAHPA
                     TRVPRWRIAAWLAGVATIGLALVSAVDVYAESLFSVHMVQHLLLAMVAPPLLALGAPM
                     TLALRAVSSNVRRSILLPVLHSSPVKAISWPPVGWTLFAIVMWLTHFSPLFNAALENE
                     TLHSAEHLLYLVAGMLFWWPVIGADPIRWRLSPIGRMAYLAAQMPFNTAVGLAIYFAP
                     TILYPHYAFLGRTWGPDPLSDQQVAGIVMWGIGDVILLGALVLAIEAWLRADEKRSRR
                     TRERANQDVERASVEGN"
     gene            6164..7117
                     /locus_tag="VIN32_04350"
     CDS             6164..7117
                     /locus_tag="VIN32_04350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921845.1"
                     /translation="MKATSLGRWAAVAAIGLVLVACSTPAGSVAPSEAGQYPGWPGNG
                     TVVANGDFIPVIVSAEVGAGHSRVLITLQDGAGRSLAAPAVTVQERFYDLASSTEIPV
                     SQVDGTFRWLIPDAKAIYTSYADFAHPGDWGIEVTGLEAGKPDRTARATFSVREKTET
                     PAIGADAPPSDTLTATDAAAIAAISTDTDPDPAFYGLSVRDAIATGKPFVLVFATPLF
                     CTSGTCGPALDLVKQVAPDYAGQVNFIHVEPYLLHVTDGRTQPTLDALGHSQPVKAVL
                     EWGLPTEPYVFVVDAHGKVAAKFEGMAYPDELTAALDAVLR"
BASE COUNT         1226 a         2531 c         2537 g          976 t
ORIGIN      
        1 ggtcacccca tagtcgattg agatgccgcc atccgcgttg agaatcgtcg gccagctgac
       61 ctgcccgtag cgggcgagga agccccgggc gccatcggtg gtgtcgttga cgagcacccc
      121 gaccatctgc agctccccgc cgtggcgctc cgcaaggtcc agcaggaccg ggtgctcatc
      181 cacgcatgag ggaatgcagt agctcgccca gaagttgacg atcgccggct tgccggccag
      241 atcggccgac gagaagtggc ccccgtcgag ggtcgttcct gcgaaggcgg gcatcggctt
      301 gccgaccagt ggtgagggga tgagccgcgg atcgtgcccg agcccggtga agagcagcca
      361 gccgacgggc aggacgacga gcgggacgag gagccagcga aggagggaga ggcgcatcgg
      421 tcaggctccc gtcgccagcg cggcctcgac cgcgatcctg cggcggtccg gccagatggc
      481 gaagagggcg cccagcgcca cgatcgcgcc gccggtccag atccacatca ccaggggatt
      541 gatgaagaca cggagggtcg ccgtgcgggt ggtcgggtcg gagccgagga gcgtgacgta
      601 caggtcctcg ccgagcgcgg tgcggacaac cggcgttgcg atcgcggttg acgagttggg
      661 gtagtcgcgc agggcggtgg caatggcccc ggactgcggg ccggccaggg tgagctcggc
      721 acgcgtctcg atgacgcgtg gatccgaggc aagcggctcg gtgacgagcc tgtcgtaggt
      781 gagcgtgtat ggcccgatgg cgaggctctc cccttctcgc agggtcgcgg ttcggtcggt
      841 gccgagcgtg gcggagacgg cgatcgccac cgccatgacg cagatgccga cgtgcgccag
      901 gtagccgccg tatcggcgcc gattccgcgt cgccagtcgg agcaccgcga ggggcgcgga
      961 ctccccgcgg gatcgtgcac gcgagcgcgc gccgcgcacg acctcgtcac ccatcaccac
     1021 cagcacgaag gtcgcaagcc caaggatgcc gagtggggct gcgccgcgca ctcctagtgc
     1081 ccaggccgcg atcacaaccc cgacgccaga caggatcgga atggcgaagc gctcccggac
     1141 ggtggcccat gaggcgccgc cccatggcag ggcaggcccg accccggtca ggaagaggag
     1201 cgccacgaag atcggcgcgt tgacccggtt gaaccacggg gcccccacgc tggcgcgcgc
     1261 ccctgacatc gcctcgacca ggatcggata cagcgtgccg aacagcaccg cgaaggtgag
     1321 ccccaggaag agcacgttgt tgaacaggaa cgccgactcg cggctcacca ccgctcccgg
     1381 cgggcgccgg tcccgcaggt cggggaggcg ccagatcagg aggcccagcg ccacgcccag
     1441 ggtcagcagg atcgcggacg cgaaccacgc tccgatcccc gacgtggtga aggcgtgcac
     1501 gctgttcacc acccctgagc gtgtcaggaa ggtcccgacc aatgtcagca cgaaggtcgc
     1561 gatcacgagc gcgtgggtcc acgtccgcag cgtgccgcgc cgctcggcaa ccatgctgga
     1621 gtggatgaag gcggtcgccg tcagccacgg cagcagcgcc gcgttctcca ccgggtccca
     1681 ggcccagtag ccgccccagc cgaggaccgc atagctccac caggcaccgg ccacgatccc
     1741 ggcggtcagg aagatccagg gcaccagcgt ccatcggcgc accaccgcga gccattcctc
     1801 gtcagtccgg cgggtgatga gcgcggccat gccgaaggcg aacgggatcg ccatgccggt
     1861 gtagcccagg tacaggagcg gcggatggag gcccatgaac gggtggttct ggagcagcgc
     1921 gtttggcccg tttccctcga ggccgatggg agtcacgcga acgaacgggt cgctcggcca
     1981 cgccatcacc gcgaagaaga atgccgtccc cagcgcgagg gtcgccccga cccacggcat
     2041 cagggcgggg aggcggctgc gatatcggta caggacgagg ctcgcccagc cggtagcgag
     2101 cagggtccag aacaggatcg atccctcgag cgccgcccac agcgagatga ccgaatagaa
     2161 gggtggcgtg gtggtcgcgt tgtttcgcgc cacgtacagg acgctgaaat cgtgggtgag
     2221 gagtgagacg atcatggcca gacatgcgaa tgcgacgagc ccgaatgccg cgtacatcgc
     2281 gagccgcgcg ctggccagca cagccgtgtc cccaccgcgg ccgccaatga cgaaggcccc
     2341 ggcagcgtag acggtcgccg ccaggccgat gacgacggcg aggtgcccca gggtcgggat
     2401 cacgagccgg ggtcgaagcc ctgggtcggc agcccgccct gggacggggc gacatagttc
     2461 tcgtcgtgct tgacgatcac ctgggacgcc tcaaagactc catcggcgcg cagctcaccc
     2521 tccaccacgg ccccgatccc ctcgcggaac gatcgggtcg gggcggcggc agcgtggacg
     2581 gagatctccg tggtcccgtc ggtcaggaca aaggccaggt caggcgcctc gcccgatatg
     2641 gttcccgcct tcaccaggcc gccgagccgc tccgtcttgc cgacctcagc cgggccgcgg
     2701 gccagcagct ccgtcggagt gaggtagtag acgagcgcgt tgccgacgct gctgaacgcc
     2761 aggtaggcga tcaccgcgac caccacggcc accccgacca gcaggcccca gcgacggcca
     2821 cgcggcggca ggggcgccag gaccggccga gccagcgtca tcgggatgcg tcaacgtcgt
     2881 cggtgcgccg cgttgcgtcg cgggcgatgc gcagccgacg cgccaggagg gcgccataca
     2941 ggccgatccc gcccaggatc acgccgtagg cggcaatcac gaagcctgcg ttcatcatct
     3001 cgccggcccc tcgacggccg ccgtgcccgg cgcagcctcg cgtgcggcga ggacgaagcc
     3061 acggcgcgca tcctcctcgc gatgccaatc gaggcgggcc agctcgtagc ggcggatgag
     3121 caggtagacc cagaccaggg tgaaggcggc aagcatggcc agcagtgcca gcacgaactg
     3181 gagcggggcg ctgggattga ggatcttgga cgggtcgccg atggtcgggc cctggtgcag
     3241 gccgcgccac cagagaacgc tgaagtgcac gatcgggatg tcgaccgccg cgatcagccc
     3301 caccaccgcc gcgcgcgccg cgcggcgagc ggggtcatcg gtcaggcggc gcaggaggag
     3361 gtagcccacg tagagagcca acaggagcgc ggtcgtcgtc aggcgcgggt cttcccactg
     3421 ccagtagacg ccccacacgg gccggcccca catcatcccg ccccagatgg cgaaggcggt
     3481 gaacatcacc ccgacctcgg caccggccag cgccagcgca tcgaggcgca ggtcccgcca
     3541 gatgaggtag ccgagcccgg ccagtgcggt cacgccgaac gaggcatagg caatccaggc
     3601 cgatgccacg tgcacgtaca tgatccgctg cgcatcgccc tggttgaggt cggcgggcac
     3661 cacgaagagg ccgttgatgg ctgcaccgat cacggccgcc agcgccacga accccaggat
     3721 gcgggtcttc atcagtcctc cagcaggaag ccgtaagtgc cggccccgac gaccagcatg
     3781 atgacgtcga acgcgaccag cagctggagc caggagccca gctcccccag cggatcgccg
     3841 ctgatcgcgg cgccagtggc cttcacggcg gcaaggagca gcggcgcaac gaccggcagc
     3901 atcaggagcg gcagcatcac ctcgcgggcg cgcatccgca ccgtcagtcc cgcgtaggcg
     3961 gtgccgatcg ccgcgaaacc gatggcgccc agagccaccg cgacgagcag cggcagccag
     4021 gccgcgccga tcgggatggc atacaggacc gccacgaccg gcaggaggag accaccgagg
     4081 acgagcatgg ccgctgctcc ggccagcgcc ttgccggcgt agatcgcgcg gcggtcgacc
     4141 gggtacaggg cgagctgctc gaacgccccg tcatcggcct cgagctggtg caggcgcgcc
     4201 atggtgagca gcccgccgaa gacgatcgcc agccagagga ggcccggcgc cgcgtcagca
     4261 aggcgccgcg agtcggggcc gagcgcgaag ccgaacagga ggaggacgag cgccgcgaag
     4321 aagagggtcg agcccgccgc ctggcggccg cgcagctcgg agatggcgtc cttgcgggcg
     4381 atcgcaacgg ccaccgccat gcctcgccgg aggtcgttca tcggtccatc ccggcgacag
     4441 ccatgcgcgg ccgtggtgag ccgcccaccg gccccgcact gacccccgca ccgctcacct
     4501 ctgccagcag cccgccctcg agccgcattg ttgcgtccgc caggctggcc acccgctcgg
     4561 cctggtgcga ggcgaccatc accgtcacgc ccgcctcttt ccagaggccg atgagccggt
     4621 ccacaagggc catcccgtcg ccgtccaggg cggcgtgcgg ctcatcgagg agaagcagtg
     4681 acggccgagc aacgaggact cgggccaggg cgaggcgctt gcgcatcccc gccgagaagg
     4741 tgcgcacccg ttgagctgcc acggcgtcca gggcgaccgt tgccagggcc tcgccgacga
     4801 cctcgtggcc ctcccgctcg ccccagccga acatcgtcgc cgcaaatcgg atgttctcct
     4861 ccgcggtcag gtcgtcgtaa tgcgccgtgg cgtgcgacag gtacacgacc cgcgggcgaa
     4921 cctgctcggc ttcgcgcagg gcgtcgatgc cgtcaatcga gacggagccg taggacggcc
     4981 ggatggcggt tgccaggatc cgcagcagcg tggtctttcc cgctccgttg ggtccgagga
     5041 gggcgaccgt tcggcccatc tcgaccgtca acgacacgcc ggcgagcgcg gcgctgcgac
     5101 caaagaggcg cgccagctcg acggtggcaa ccgccggagg gatggtccca agcgtcccgg
     5161 cgagccgggg cgcgacgtag atggtggtct gcggcatgct cgacgggggg cggatgatgg
     5221 atggaggctg agtgtgcggc ccggggtagt gggcgtcaac tcgggcgacg gcgccgaaat
     5281 aggcgatcat gagggatgct ccgcgcgccg agcctcggaa tcatcctctc gacctggtcc
     5341 ttcgatcccc tgccatggat cgggatcgtg ctggctgccg ctctctacgt ggcggcggtg
     5401 cggatcgtcg atcgagctca cccggcgacg cgggtgccga ggtggcgcat cgccgcctgg
     5461 ctggcgggcg ttgccaccat cgggctcgcc ctcgtctccg cggtcgatgt ctacgccgaa
     5521 agcctgttca gcgtgcacat ggtccagcac ctgctgctgg cgatggtcgc cccgccgctg
     5581 ctggcgctcg gtgcgccgat gacgctcgcc cttcgcgccg tcagctcgaa cgttcgccgc
     5641 tcgatcctcc tcccggtcct gcactcctcg ccggtcaagg ccatctcctg gcccccggtc
     5701 ggctggaccc tcttcgccat cgtcatgtgg ctcacccact tcagcccgct cttcaatgcg
     5761 gcgctggaga acgagacgct gcattcggcg gagcacctgc tctacctcgt ggcgggcatg
     5821 ctcttctggt ggccggtgat cggggccgac ccgatccgct ggcgactgag ccccattggg
     5881 cggatggcct acctggccgc ccagatgccg ttcaacaccg ccgtcgggct cgcgatctac
     5941 ttcgcgccga ccatcctgta ccctcactac gcgttcctcg gcaggacctg gggacccgat
     6001 ccgctcagcg accagcaggt cgccggcatt gtcatgtggg ggatcggcga cgtgatcctg
     6061 ctcggtgcgc tggttctcgc catcgaagcc tggctgaggg ccgatgagaa gcgcagtcgc
     6121 aggacccggg agcgagccaa ccaggatgtc gaacgagcgt ccgttgaagg caactagcct
     6181 tggccgatgg gcagcggtgg cagccatcgg tctggtgctc gttgcctgct ccactccggc
     6241 aggcagtgtc gcgccatcgg aggcagggca gtaccccggg tggccgggaa acggcacggt
     6301 ggtcgccaac ggcgacttca tccccgtcat cgtctcggcc gaggtcggcg ccgggcacag
     6361 tcgagtgttg atcacgctgc aggacggcgc cggccgttcc ctcgccgctc ccgcagtcac
     6421 ggtccaggag cgcttctacg acctggccag cagcaccgag attccggtca gccaggtcga
     6481 cggcaccttt cggtggctta ttccggacgc gaaggcgatc tacacctcct atgccgattt
     6541 tgcccatccc ggcgactggg ggatcgaagt caccgggctg gaggcgggca agccggatcg
     6601 aacggcccgc gcgaccttct cagtgcgcga gaagacggaa acgccggcca tcggtgcgga
     6661 cgcgcctcca tcggacaccc tcactgcgac cgatgcagcg gccatcgcgg cgatctcgac
     6721 cgataccgac cctgatcccg ccttctatgg cctctcggtc agggacgcca tcgccaccgg
     6781 caagccgttc gtgctcgtct tcgccacgcc cctgttctgc accagcggca cgtgcggccc
     6841 ggcgctcgac ctcgtcaagc aagttgcgcc ggattacgcg gggcaggtga acttcatcca
     6901 cgtcgagccg tacctgctcc atgtgaccga tgggcgaacg cagcccacgc tcgacgcgct
     6961 cggccattcg cagccggtca aggcggtgct ggagtggggc ctcccgacgg agccatacgt
     7021 gttcgtggtg gacgcccacg gcaaggtcgc cgccaagttc gaggggatgg cctaccccga
     7081 tgagctgacc gccgcacttg acgcggtcct gcgctgaggc gcggcttaca ggacgtagag
     7141 ggtcaaaaag aggaagaccc ccgcggcaac gacgaagtgc cagtagtagc tcgtcgcctc
     7201 cacaatggtg tggtgctcgg ccgagacgag tccacggcgc ctgcgcgtgg cgacaatcgc
     7261 catcagccca
//
LOCUS       DATCQR010000129         6642 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_223788, whole genome shotgun sequence.
ACCESSION   DATCQR010000129 DATCQR010000000
VERSION     DATCQR010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6642
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223788"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            98..1246
                     /locus_tag="VIN32_04355"
     CDS             98..1246
                     /locus_tag="VIN32_04355"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HEY8921846.1"
                     /translation="MSSNGPAEDGRVDLRDYYEVLGAEPSASVAELRAAFREAVLRHH
                     PDRSSEDSLATRRTSVLNRAWGELRDPARRLHYDHALERGGAATLAWPLGADEAPSPV
                     RRPRRAARPLRTPWHTPEFRSVLGFRVPTQVFMAGPAAQRAWIVANQIDGQDWRDHRE
                     RYWLRFAAAYYRDRGRSDDWLGAMERLVELDGTFETLVRADLRAAYVAAQEYLRGTGF
                     LRTVAMPYTSGSAQRKWIDRELRALLAEYRDARVRKGRPEDRAEAAELLLNYLEALEM
                     PPALADVRAAIMAHLRAGHAGRAAELLERAVASPITEPARWFSMVQLLTEGGQLDRAS
                     AMLAEIARGDHPEALNRRRLRGDPARRISAARGRLERARQRAAARAGS"
     gene            complement(1243..2601)
                     /locus_tag="VIN32_04360"
     CDS             complement(1243..2601)
                     /locus_tag="VIN32_04360"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEY8921847.1"
                     /translation="MTTAVNPMRPAAGVPRPYHFPPFARRQLANGLNVWTIPVPGSAL
                     MNVHLLVDAGAGAEDEPQGGIAALTSQLLVTGTKRLDASEFAEATERLGIEVSSESSW
                     DSARGAFQSLPRHLDEGLGLLSEMMREPRFDAGEFERLKAERLTDILQSRSDPGGLAD
                     ETFLMHAYAADTPYRRLSAGNPETVEALTRDDVIDFHRTHYLPGLAHLVVAGSFDDDA
                     VEEAVERHLASWEGTGPGHRTIVPRPGGARRIVIVDRPGSVQSELRVGHIGIDRYDPR
                     YFPAIVMSTALGGMFGSRLNRRLREELGYTYGARSGFDARRSAGVFTAATAVQTDVTA
                     PAIGELVALLEGARTAPFDESELADARNFQIGIFPLRFESTGGIAAAIEPIAVYGLAD
                     DFWQTYRQKLEAVGAAEAHSAAMELIRPDELLILAVGDADVIRGDVEALGIAPVEVVP
                     AP"
     gene            complement(2598..3893)
                     /locus_tag="VIN32_04365"
     CDS             complement(2598..3893)
                     /locus_tag="VIN32_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020555824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEY8921848.1"
                     /translation="MPHIPIDRHQLPNGLRVILSRDQRAPIVAVNLWYDVGSRHEKPG
                     KTGFAHLFEHMMFQGSANVAKGEHFDLVHGAGGSVNASTWLDRTNYFETMPSHELELA
                     LWLEADRLGSLLPAMTQEKLDNQRDVVKNERRLRVDNLPYGSWDEKLLGLLFPEDHPY
                     HHSTIGSMEDLDAASLDDVREFFAAHYAPNNAVLTIAGDFEPGPALNMVEKHFGPIPA
                     NPAIPEAPSTSVDPLIGSEVREVVPDRVPLPRIYAAYRMPTFGTDGFNALEVAVDLLG
                     SGRASRLYRALVREQQLAQDVTTFAFPVVGGAAVFTIWATARPGVAHEALEAALWGEV
                     DRLTGDGPTDDELERVRNLHALEVESSLERISERADRLSMYACLFDEPEQINTEVSRY
                     EAVDAGRVREAMSASLRPDNRVVLTYLPAESPESTEGAA"
     gene            complement(3980..4561)
                     /locus_tag="VIN32_04370"
     CDS             complement(3980..4561)
                     /locus_tag="VIN32_04370"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011754924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEY8921849.1"
                     /translation="MSEAERQIWLARHGETEWSRDGRHTGLTEIPLTNLGRSQAEALG
                     ARLAGHRFSLVLTSPRSRASETARLAGFADGEVDPDLAEWDYGDLEGRRSAEIAADHP
                     GWTIWTGPWPGGETIGQVSARADRVLARCLAPDVTGDTLIFGHGHMLRVLAARWLDMP
                     GQSGSLFGLSTATLSVLGWEREHRIIESWNVPS"
     gene            complement(4558..5151)
                     /locus_tag="VIN32_04375"
     CDS             complement(4558..5151)
                     /locus_tag="VIN32_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009109528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HEY8921850.1"
                     /translation="MTKGRLEAFSDGVIAIIITIMVLELRPPEGARFENLEPLIPTFL
                     SYLLSFALLGTWWNNHHHLLQAAKVIDARVLWANLHLLFWLSLFPFGTAWVGQTTFAP
                     IPVATYTGLLLGAGMAYYVLVRALIAAHGQPAALAEAVGRDVKGKASSVLFAIAIPIA
                     LVAPIVAMLINVGVVVMWLVPDRRMERVIRSDRPNPP"
     gene            complement(5365..5718)
                     /locus_tag="VIN32_04380"
     CDS             complement(5365..5718)
                     /locus_tag="VIN32_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005475512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY8921851.1"
                     /translation="MVRIGSIVLRVDDVRRQAEFWAAALDYVAREEPDDSSQMLSPRD
                     GVGPNLNLDHRRSDAQIPPRMHLDLYAEDQAAEVSRLIGLGASEVPWDKRPPDADFVI
                     LADPEGNRFCVIDRG"
     gene            complement(5802..5876)
                     /locus_tag="VIN32_04385"
     tRNA            complement(5802..5876)
                     /locus_tag="VIN32_04385"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5842..5844),aa:Lys,seq:ctt)
     gene            5982..6293
                     /locus_tag="VIN32_04390"
     CDS             5982..6293
                     /locus_tag="VIN32_04390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921852.1"
                     /translation="MKTLRGFLSPWALAALAVWIVVMVLAYNWAVPIYANIGGPGLCP
                     VNTPNCSPETFTVNVPGTAIFAVIAASIVTALLAGVVFLVLRLTGRAPASLHHREREG
                     R"
BASE COUNT         1043 a         2273 c         2290 g         1036 t
ORIGIN      
        1 cgccgccgat gatcagcagg tgacctgggc gggcggcgcc cgggggtgag ggttgggcca
       61 tgttgccggg cagcatagca acgctagact cgatcggatg tcctccaatg gtcccgccga
      121 agatggtcgc gtcgatctcc gcgactatta cgaagtgctc ggcgccgagc cgtcggccag
      181 cgtggccgag ctgcgcgccg cctttcgcga ggcggtgctg cgccaccacc cggaccgcag
      241 ctccgaagat tccctcgcca cgcgccgcac gtcggtcctg aaccgcgcct ggggcgagct
      301 ccgcgatccc gcccggcgcc tgcactacga ccatgccctc gagcgcggcg gcgccgcgac
      361 gctggcctgg ccgctgggcg cggacgaggc accatcaccg gttcggcggc cgcgccgcgc
      421 cgctcggccg ctgcgcacgc cgtggcacac gccggaattc cgcagcgtcc ttggcttccg
      481 cgtcccgacc caggtcttca tggccggtcc ggccgcgcag cgagcgtgga tcgtggccaa
      541 ccagatcgac ggccaggact ggcgggacca tcgggaacgc tactggctgc ggttcgccgc
      601 ggcctactac cgcgaccgcg gtcgaagcga cgactggctc ggcgccatgg agcggctggt
      661 cgagctcgac ggcacgttcg agaccctggt ccgcgccgat ctgcgcgccg cctacgtcgc
      721 cgcgcaggaa tacctgcgcg gaaccggctt cctgcgcacg gttgcgatgc cgtacacatc
      781 cggcagcgcg cagcggaagt ggatcgaccg cgagctgcgc gcattgctgg ccgagtaccg
      841 cgatgcccgc gtgcgcaagg gccgccctga ggaccgcgcg gaggcggccg agctgctgct
      901 caactacctc gaggcgctgg agatgcctcc cgccctcgcc gacgtgcggg ctgcgatcat
      961 ggcccacctg cgcgcgggcc acgccggccg cgccgcggag ctgctggaac gcgcggtcgc
     1021 ctcgccgatc acggagccgg ctcgctggtt cagcatggtg cagctcctga cggagggcgg
     1081 acagctcgat cgggcctcag ccatgctggc cgagatcgcc cgcggcgacc accccgaggc
     1141 gctcaaccgt cgccgcctgc gcggcgatcc ggcccgtcgc atctcggctg cccgcggtcg
     1201 tctcgagcgc gcccggcagc gcgctgcagc ccgcgccggg tcctaggggg cgggaacgac
     1261 ctcgaccggg gcgatgccca gcgcctcgac gtcgccgcga atcacatctg catcgccgac
     1321 cgccaggatc agcagctcgt ccgggcggat cagctccatc gccgcgctgt gcgcctcggc
     1381 cgcaccgacc gcctcgagct tctggcgata ggtctgccag aagtcatcgg ccaggccgta
     1441 caccgcgatc ggctcgatcg ccgccgcgat gccacccgtt gactcgaagc gcagcgggaa
     1501 gatgccgatc tggaagttac gcgcatcggc cagctccgac tcgtcgaacg gcgcagtgcg
     1561 tgccccctcc agcagcgcca ccagctcgcc gatggcgggc gccgtgacgt cggtctgcac
     1621 cgcggtcgcg gccgtgaaga cgcccgctga ccgccgcgca tcgaagccgc tccgcgctcc
     1681 gtacgtgtat ccgagctcct cgcgcagccg ccggttgagg cgcgatccga acatgccgcc
     1741 cagggcagtt gacatgacga tggccgggaa gtagcgaggg tcgtatcggt cgatcccgat
     1801 gtgcccgacc cgcagctcgc tctggaccga tccgggtcga tccacgatca cgatccgccg
     1861 cgctccaccg gggcgcggga cgatggtgcg gtggcccggc ccggtcccct cccagctcgc
     1921 cagatgacgc tcgacggcct cctcgaccgc atcgtcgtca aaggagccgg caacgacgag
     1981 gtgtgccagt ccgggcaggt aatgggtcct gtggaagtcg atcacgtcat cgcgggtgag
     2041 ggcttcgact gtctcggggt tgcctgccga caggcgacgg tagggggtat cggcggcgta
     2101 ggcgtgcatc aggaacgtct cgtccgcgag ccctccgggg tccgagcgcg actggaggat
     2161 gtcggtcagc cgctccgcct tgaggcgctc gaactcgccg gcgtcgaagc ggggctcgcg
     2221 catcatctcg gacagcaggc cgagcccctc gtcgaggtgc cgcggcaggg actggaaggc
     2281 accgcgagcg ctgtcccagc tcgactcgct gctgacctcg atccccagcc gctccgtggc
     2341 ctcggcgaac tccgacgcgt ccagacgctt cgtgcccgtc accagcagct gggaggtcag
     2401 cgcggcgatc ccgccctgcg gctcatcctc ggccccggcc ccggcgtcca ccagcaggtg
     2461 cacgttcatc agggcggagc cggggaccgg gatggtccag acgttgaggc cgtttgccag
     2521 ctgccggcgc gcgaagggcg ggaagtggta ggggcgaggg acgcctgcag ccggccgcat
     2581 cgggttcact gcggtggtca tgccgcaccc tcggtggatt cgggggactc agccgggaga
     2641 taggtcagca cgacccggtt gtccggcctc aggctggcgc tcatcgcctc gcgaacgcgc
     2701 cccgcgtcaa ccgcctcata gcggctcacc tcggtgttga tctgctccgg ctcgtcgaag
     2761 aggcaggcgt acatggaaag ccgatccgcc cgctcgctga ttcgttccag gctggactca
     2821 acctccaagg cgtgcaggtt gcggacacgc tccagctcgt catcggtggg gccgtcgccg
     2881 gtcaggcgat cgacctcacc ccagagggcg gcctccagcg cctcgtgcgc gacgccgggt
     2941 cgcgcggtgg cccagatggt gaagaccgcc gctccgccga ccaccgggaa ggcgaacgtg
     3001 gtcacgtcct gagcaagctg ctgctctcgc acgagggccc gatacaggcg cgacgcgcgc
     3061 cccgagccga gcaggtcgac cgccacctcg agcgcattga agccatcggt gccgaaggtt
     3121 ggcatccgat aggccgcata gatccggggc agcggcacgc ggtcggggac cacctcgcgc
     3181 acctccgagc cgatgagcgg gtcgacagat gtggatggag cctcggggat cgccgggttg
     3241 gccggaatcg gtccgaagtg cttctcgacc atattcagcg ccggtccggg ctcgaagtcg
     3301 cccgcgatcg tcagcaccgc gttgttgggt gcgtagtggg cggcgaagaa ctcgcgcacg
     3361 tcgtcaagcg aggccgcgtc gaggtcctcc atcgagccga tggtggaatg gtggtaggga
     3421 tggtcctcgg ggaagagcag ccccaggagc ttctcgtccc acgacccgta cggcaggttg
     3481 tcgacccgca agcgccgctc gttcttgacc acgtcgcgct ggttgtccag cttctcctgc
     3541 gtcatcgccg gcagcaggct gccgagccga tctgcctcca gccacagagc cagttccagc
     3601 tcgtggctgg gcatggtctc gaagtagttg gtgcgatcca gccaggtgga ggcgttcacc
     3661 gaaccgccgg ccccatgcac caggtcgaag tgctcgccct ttgcgacgtt ggcggacccc
     3721 tggaacatca tgtgctcgaa gagatgcgcg aacccggtct tgcccggttt ctcgtgcctg
     3781 ctgccgacgt cgtaccacag gttcacagcc acgatcggtg cgcgctggtc gcgtgacagg
     3841 atgacgcgca ggccgttcgg cagctgatgg cggtcaatcg gaatgtgggg caaggcgtca
     3901 gtctccgggt tgttggcgga agcggcggaa ggctaccacg cgccgatggg atgcctccga
     3961 gtgccggctc tgcgccggac taggacggca cattccagct ctcgatgatc cggtgctcgc
     4021 gctcccagcc caggaccgac agggtggccg tcgacaggcc gaagagggag ccggattgcc
     4081 ccggcatgtc gagccagcgc gctgccagca cccgcagcat gtgtccgtgc ccgaagatca
     4141 atgtgtcgcc ggtaacgtcg ggggcgaggc atcgggccag gacgcgatcg gcccgcgcgc
     4201 tgacctgtcc gatcgtctcg cctccgggcc aggggccggt ccagatcgtc cagccgggat
     4261 ggtcggcggc gatctccgcc gagcgccggc cctcgaggtc gccgtagtcc cactcggcga
     4321 ggtccgggtc gacctctcca tcggcgaatc cagccagccg ggctgtttcg gaggcgcggg
     4381 accggggact ggtcaggacc agggagaatc ggtggccggc aagccgggcc cccagcgctt
     4441 ctgcctggct gcgcccgagg ttggtcagag ggatctccgt caggccggta tggcgcccgt
     4501 cccgggacca ttcggtctcg ccgtgccgag ccagccagat ctgccgctcc gcctcgctca
     4561 cggggggttg gggcgatcag atcggatgac gcgctccatg cgccgatccg gcacgagcca
     4621 catcaccacc acgccgacgt tgatcagcat cgcgacgatc ggcgccacca gcgcgatggg
     4681 aatggcgatc gcgaacagga cgctggaggc cttccccttc acgtcccttc caaccgcctc
     4741 ggcgagcgct gcaggttgac cgtgtgcggc gatcagcgcg cggaccagga cgtagtacgc
     4801 catgcccgcc ccgagaagca gtccggtgta ggtcgccact ggtatcggcg cgaaggtggt
     4861 ctggccgacc catgcagtgc caaacgggaa gagggagagc cagaagagca ggtgcaggtt
     4921 ggcccacagc acccgcgcgt cgatcacttt cgctgcctgg agcaggtggt gatggttgtt
     4981 ccaccaggta cccagcagcg cgaagctcag gaggtagctg aggaaggtgg ggatgagcgg
     5041 ctcgaggttc tcgaatctgg cgccctccgg cggccgcagc tccaggacca tgatcgtgat
     5101 gatgatcgcg atgacgccat cgctgaacgc ctcgagccgg cccttggtca cccgcgcatg
     5161 ctagccgcgt cccgcgtcgg ggcaacggaa aagcccgaag gggcccgatc actagctgct
     5221 ccagcccatg caaaacacaa actgaatcac tagatgttcg aggctcctcc gagccaccta
     5281 cacgttagca ccgcggccag cgacgccaac ctttcggtcc gcgggaagcg catatcagcc
     5341 gccttgcagc tgagccagcg gacgctaccc cctgtcgatc acgcagaagc ggttcccctc
     5401 aggatcggcc aggatgacga agtccgcatc tggcggtcgc ttatcccatg gcacctcgga
     5461 ggcgcccagg ccgatgagcc ggctgacctc cgcagcctgg tcctcggcgt acaggtccag
     5521 atgcatccgc ggcgggatct gagcgtccga gcgacgatgg tcgaggttga ggttcggtcc
     5581 cacgccgtct cgagggctca gcatctgtga gctgtcgtcg ggctcctcgc gggccacata
     5641 gtcgagtgcc gctgcccaga actccgcttg gcgccgcacg tcatcgacac gcaggacgat
     5701 cgagccgatc ctgaccatca accgagtata ggcggcgtgt gagcctccgg aacacgacga
     5761 agccggaccc gctggaggcc cggttttgtg ctgagaatga gtggtgagcg gccagggatt
     5821 cgaaccctga acctagtgat taagagtcac ttgctctgcc gttgagctag ccgcccgcaa
     5881 cggaatcatg ggggagtcag acgagagggt caaccacggc cattgcagcg gcacaggcgt
     5941 gagctcttgt agagggctcg ggctcgcagt acggtgcgga catgaagacg ctgcgcggct
     6001 tcctctcgcc ctgggcgcta gccgctcttg ccgtgtggat cgtggtgatg gtgctcgctt
     6061 acaactgggc ggtcccgatc tacgccaaca tcggtggccc cgggctatgc cccgtcaaca
     6121 cgccaaattg ttcaccggag acattcacgg tgaatgtgcc gggcacggcg atcttcgcgg
     6181 tcatcgcggc gagcatcgtc acggcgctat tggcgggggt cgtgttcctt gtcctgaggc
     6241 tgactggccg cgcgccggct agcctccacc accgagagcg cgaaggtcgt tagaggagtc
     6301 cacgcggcgc cgggcgtcgt ggcggcgccg gtcccaaagg gcgaaggttg tagcagcctg
     6361 cagataagcg tggcttatgg tcctgcacgg cagccctccc atcggtgccg tttcataagc
     6421 caggcttcta caacttcacc gacggagcct catcggtgca cgagcattag ccagccttat
     6481 cggctgtcgg ctgcgaccgt caggtcccgg gtttagcggg gccaccgaac ggctcgggcg
     6541 cgtcgccgta atgggcgtcg ctgaaggccc ggatcttgtc caggtcggcg gtgtcgaggt
     6601 agaggagcca gttccaggcg gtcaccgcga tcgggtggcc ga
//
LOCUS       DATCQR010000130        18085 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_147191, whole genome shotgun sequence.
ACCESSION   DATCQR010000130 DATCQR010000000
VERSION     DATCQR010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 18085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18085
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147191"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..738)
                     /locus_tag="VIN32_04395"
     CDS             complement(67..738)
                     /locus_tag="VIN32_04395"
                     /inference="COORDINATES: protein motif:HMM:NF017734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HEY8921853.1"
                     /translation="MKSRVAQATLFAVAMLIGLLGVGQLNSQARPIAVSRLSATELST
                     LIETLTARNRELRSGLADIREQLRQYEVSGPQGDSALQVSREDLRRITAFGGLAAVDG
                     QGIVMDVDGDLDAIAVNDLINELRNAGAEAIAVDGVRITARSVAIEGPRAIELDGVEV
                     GAQFTLRAIGSPDGLLGAMERPGGIISQLELFIKATIQIRQATSVQLPASAVSLQPVI
                     GKPVE"
     gene            complement(738..1490)
                     /locus_tag="VIN32_04400"
     CDS             complement(738..1490)
                     /locus_tag="VIN32_04400"
                     /inference="COORDINATES: protein motif:HMM:NF017734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HEY8921854.1"
                     /translation="MTARRRRLLVSPQWAVSIAGALAVIGFVGYAQWNGSPERVQFAT
                     SVQQVLADQVSKLEAEQEDLRGQLAAAEAQVLDFQKASTTSTSALADVNRRLEEVRLA
                     VGLAPLRGPGVIIEIADSKRVIPDGENPANYIVLVDDLRDIVVALWASGAEAITINGE
                     RLVATSSIYGVGASVLVNTAFLSPPFRIEAIGAEGLLDRFQTNPAFLGRVAQRIDVFG
                     LEFATQRADAVELAAFVGNTRFRWAVPTEGLP"
     gene            complement(1487..2779)
                     /locus_tag="VIN32_04405"
     CDS             complement(1487..2779)
                     /locus_tag="VIN32_04405"
                     /inference="COORDINATES: protein motif:HMM:NF024876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase MA family metallohydrolase"
                     /protein_id="HEY8921855.1"
                     /translation="MRRAIRAAAIVLALTIAVPLPAAAFTGFGSMTADATYGGQMRFT
                     VQLPSGPPDQLALLLRFAGSDSTLVVPVDPGPSSAEFVRDTANQPITPNTSIAYRWRA
                     TVGDKVTLSDEKTLLYDDDRPGLDWHSAQIGAATVHWYGGAEAQARHFGSVSADAAAA
                     AEALLGHDLAGPIDIFVYDSHDDFFGALGPGAREWTGAATFPELRTIFMWLQGGSSSY
                     LETTMTHEVTHVVFQDATANAFHQPAKWFNEGLAVWSEQQSASGHEGGVRSAADARGM
                     LAFDGITESFPIGDQAARLAYDEGATMVAMIIDTYGRRAIAAIAAAWRDGAGDAEALE
                     AGTGVKADQLYDTYFASFGVAAPTAVEPAPILPSNVDKPPQPGAPAGESAAPAASSVP
                     EPAQSDGDTGWIPVVVLAGLALGVVVALVRVRRRRASP"
     gene            complement(2779..4353)
                     /locus_tag="VIN32_04410"
     CDS             complement(2779..4353)
                     /locus_tag="VIN32_04410"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY8921856.1"
                     /translation="MRRLTSRGRLPRLVAGFAAILLAFTSSLPAHPAFAVEPPLRYVG
                     GEPATLDPAFIDSAGDVQFLLQLYAGLTRLDEDGKPYASLAESWTVSGDGRTYTFHMR
                     SGLTFSDGTPLDASDVRRSWLRILDPATHSTAPDVLSIIEGAAERLAGGPEDGVAIDA
                     PDARTLTVRLRHPAAYFTAITATPTAFVVPRNASGSNDSWQSSDSFIGSGPYVARGTD
                     AGDLVLRANDRYVAGPPPIAEIRWVTNLTGDSATAFANDEVDLTSVGSFDAGWIAYDH
                     DLGPSLHRSAALGIQFFGFDTTRPPFNDARVRRAFALALDRPRLVTNAEGSSGTPASS
                     LVPPALWPEGLKDDQVTDTEQARSLLDAAGYTDRARLGTITVNGSGLGVGPAVAIWEK
                     ELGVKLVIETMEFRDYLRALVVDPPQVFTINWIADYPSPYALYSLLLLPDATSNYGGW
                     KDPEFVRLLAAASAAGSEADQAVAYRAVDDRVDSEVPVIPWAYGAGWWLARPGLRGLG
                     NLTTGLLDLGRVSWEQ"
     gene            4376..4912
                     /locus_tag="VIN32_04415"
     CDS             4376..4912
                     /locus_tag="VIN32_04415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921857.1"
                     /translation="MQPTHDHRFRPQPAGSLTLRQFVESLRRWTPEMRQVAQIALTRS
                     ETERQQYLQAFHARHHDREPKPIDSFVRWAYSDPRLDHVPALLAEIERGDDEYEEALD
                     HEADVLVRTRAHGKTPAERVHDYLALLGMEGSESVRTAIGSLLPPATSDAAVVATVRQ
                     MRRRWLDLFGDDPFFAEE"
     gene            4917..5627
                     /locus_tag="VIN32_04420"
     CDS             4917..5627
                     /locus_tag="VIN32_04420"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HEY8921858.1"
                     /translation="MDVSLALAFAAGLLSFVSPCVLALVPVYLAFLGESATVAGSGTA
                     TLNWRGPVFTQALLFVAGFAVIFVLLGTSVGLFGSFLFRIDAARQLAGAVVIGIGLLT
                     TGIFGPVLDRFMRFGVRTDRLPDARAARALTLGALVAIGWTPCIGPVLGAILTMGASS
                     RDAWVAALLLIAYSAGLAVPFLAAALALPRMQPLLNALRRHHRVVQVVSGLFIMVIGV
                     LILTNAFARMAGLFTFFI"
     gene            complement(5755..5976)
                     /locus_tag="VIN32_04425"
     CDS             complement(5755..5976)
                     /locus_tag="VIN32_04425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921859.1"
                     /translation="MSQTNVNVPGGSSDEGMGAGMIVGLILAVIIIGFLIWWFLLNGG
                     GGSSTPSNSTAPLQSVVQSVVQSILPTGS"
     gene            complement(6108..6183)
                     /locus_tag="VIN32_04430"
     tRNA            complement(6108..6183)
                     /locus_tag="VIN32_04430"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6148..6150),aa:Arg,seq:cct)
     gene            6212..6460
                     /locus_tag="VIN32_04435"
     CDS             6212..6460
                     /locus_tag="VIN32_04435"
                     /inference="COORDINATES: protein motif:HMM:NF017574.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HEY8921860.1"
                     /translation="MAVPSRVTLYERDGCHLCDDARVLLDEMLGSHGYARVDIDADDA
                     LVLRYGFRVPVIGLDGVDRLEAPITAPDLRALLAEPDA"
     gene            complement(6475..7293)
                     /locus_tag="VIN32_04440"
     CDS             complement(6475..7293)
                     /locus_tag="VIN32_04440"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY8921861.1"
                     /translation="MRAGWFRPDAGGFFGVVPRPLWSRLVEADERGRVLCRLNLLLVE
                     AGGKRVLIETGTGVRMTDKDRDIKGVEGGDPAAALRAVNVDPASIDFVVVSHLHYDHA
                     GGMVDVDGNPNFPGARYVVQRDESEAAHGDELRLAGIMEREQLDLVRGAGQLAEVQGE
                     VELVAGVSVLPTGGHTRGSQAVLLGTRSEGRDEDPGERAIFWGDLIPTRWQIPVRWTS
                     AFDDYPIQAVEIRNELVTRAAAEGWWCYFTHDPGELPIQIEATAKGFQVRSEPS"
     gene            complement(7399..7980)
                     /gene="dcd"
                     /locus_tag="VIN32_04445"
     CDS             complement(7399..7980)
                     /gene="dcd"
                     /locus_tag="VIN32_04445"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017563902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HEY8921862.1"
                     /translation="MVLSDRDIRAEITAGRIVIDPFIPEAVQPSSVDLHLDRRFRVFR
                     NSRYPFIDVRADQPELTELVEIGGDDPFILHPGEFVLGSTFERVGLPNDLVARLEGKS
                     SLGRLGLLIHSTAGYVDPGWDGNLTLELSNVANLPITLYDGMKIGQISFQRLSSPAEV
                     PYGDASIGSKYRGQRDPTASLYHRDFERGRITR"
     gene            complement(7980..8450)
                     /gene="nrdR"
                     /locus_tag="VIN32_04450"
     CDS             complement(7980..8450)
                     /gene="nrdR"
                     /locus_tag="VIN32_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HEY8921863.1"
                     /translation="MLCPRCNEASTRVIDSRDLEGGGTIRRRRECEECSYRFTTYERP
                     EGARLAVLKRDGSRQEFDRGKLQAGLMRALEKRPVTLARVEQAVDDIEGGLRARGESE
                     VTSKEIGRLATEALRDIDQLAYIRFASVYHSYEDISTLKREVDRLMKQRTPADS"
     gene            complement(8548..9060)
                     /locus_tag="VIN32_04455"
     CDS             complement(8548..9060)
                     /locus_tag="VIN32_04455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921864.1"
                     /translation="MGLLRRRGPKRPKAHGRELQAYRNGLAEVTTMVDLELAMLGDLV
                     DALRRRDADPDEVLVDLQAGEEKLDLAADSLRDMLAPEELHALHAEYEGSLERALRGI
                     VTAERGCGITRLPYRPPDDDEPLIYWKRGHLNILHARLRMQEVVDTLLTWEPGMPAEA
                     TVGARLGRAR"
     gene            complement(9065..10045)
                     /locus_tag="VIN32_04460"
     CDS             complement(9065..10045)
                     /locus_tag="VIN32_04460"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEY8921865.1"
                     /translation="MSSDVAFLAEFSGGSPTHAALELAAGAAELAGQSKGSAVALAYG
                     PGAADAAALGSAGASRVVILGDDPSPAITHGAAAAAAVRGIGPIALLAPATPNGRDLA
                     AALVGLLGVPAFGPVRAVRIEGGRVRTEQATLQGTVITVSEPTDEAEQPAIVLVLPGT
                     FSPLEGGSGKAAVEPAPASEAGALGSASIVESHPAEAAVANLEEASVIVAGGRGVGSA
                     DGFAPLQELADALGGAVGASRAAADAGWVPYQLQIGQTGKVVKPALYVGCGISGAIQH
                     RVGMQTAENVLAINKDPDAPIGEFADLFVVGDLFAIVPKLTAEIRRRKGR"
     gene            complement(10045..10818)
                     /locus_tag="VIN32_04465"
     CDS             complement(10045..10818)
                     /locus_tag="VIN32_04465"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEY8921866.1"
                     /translation="MVCIKQVPATTAEKRYTADLRLDRAAAEAVINPLDEYAIEQALR
                     LQESGAVESVGFLSMGPEQAAEALRRALAMGGDDATLVTDPALAGADAWATARVLAAA
                     LDKLAPDVALMGMASDDARGSLVPGAVAAMRGVPLLSYGSELRIEGSAARVRRLTADG
                     FDIVEAPLPVVASVTDQVGEPRYASLKGIMAAKRKPMETWSLADIGLSPDDLHGSALT
                     RVVETRKPEPKPPTERIENVSADEAVTRIADWLASRKLI"
     gene            complement(10850..12019)
                     /locus_tag="VIN32_04470"
     CDS             complement(10850..12019)
                     /locus_tag="VIN32_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY8921867.1"
                     /translation="MTYRPTADHDELRAVVRELADERVAPRAAEIDATSEFPWDLKEL
                     LAKQDLLGIAFPARFGGTELDDISQAIVVEEIARADATTSLIVIVQKLGALPILIAGS
                     EEQQARYIPRLASGEWMAAFGLTEAGAGSDVAGIRMRARRDGDDYLLNGSKRFITHGS
                     IANLVTIIARTDADVGARKGLSAFVVETDSPGFSAPRLEHKMGIRGSPTAELHFDDVR
                     VPAANLLGAEGDGFAVAMSTLDRSRLSIAAQAVGIAQGALDFAVGYAGQREQFGERIA
                     DMQGIQFMLADMASAVEAARQLTYAAAAAVDANAPDLAYWTSSAKLIAGDMAMRVTTD
                     AVQVLGGYGYVTEYPVERMMRDAKITQLYEGTQQIQRMIVARQLLRRAGVSRPSR"
     gene            complement(12016..12882)
                     /locus_tag="VIN32_04475"
     CDS             complement(12016..12882)
                     /locus_tag="VIN32_04475"
                     /inference="COORDINATES: protein motif:HMM:NF021505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HEY8921868.1"
                     /translation="MLERWARPWARLWNGTAGVMDAIYRRLGRPGKLLQDLLNGSWLG
                     HSLHPVVVDVVVGGSTAAVLLQVLSWLGVGDLRVAILWVLALTWLAGLSAIVSGLTDF
                     KDTATGDERHVVGLHGLINILATTVLIGAFLGLLADADLLAGVLIVAGFAILSVGAFI
                     GGHIVFKYGYMVNHNAFSGGKKAKEYAAILPEADLAEATPTKAMLGPTALVLVRRGDL
                     VFALKETCSHAGGPLSQGTLDGYTVICPWHGSAFRLSDGAVRHGPAATRQVAYRARIN
                     GDQVEVQGPVIG"
     gene            complement(12897..14327)
                     /locus_tag="VIN32_04480"
     CDS             complement(12897..14327)
                     /locus_tag="VIN32_04480"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEY8921869.1"
                     /translation="MAVIERREVDRARVKAIVAREEAAYRGRTPRSAELHKRALESMP
                     LGVASSFQTYDPYPLFISEGRGSRMWDADGNEYLDFDMALGVLAAGHSHPEFVEAIQR
                     RAAIGTAFTFPIEEQILVAEELKRRFRADLVRFSNSGTEATMDAIRVARGFTGREKII
                     KFEGGYHGHHDDVLISIQPPRDMIGDVDSPNSVPTSAGIPRSRIAETIIAPFNQPEIV
                     ERILERHRGEIAAIIVEAVQFNIGVVPPEPGFLERLRELATAHGAVLIFDEVKTGVVI
                     AYGGATEYFDVQPDLFCLAKSIGGGTPIGAFGGREEVMRVIEGGARAPLIEAELSHSS
                     FGGTSRVAHMGTMNGSPLVMAAALAVLTRVLTRDVYPRLQAMGDRLTAGSQEIVDQYG
                     LPGYAINVGPKGIVMFAPKRVTGYRDFIGLDDELWASSFWFLANRGILIPPAPDDQWT
                     LSVQHTDDEIDRYLAVFREWAVEVTG"
     gene            complement(14404..16581)
                     /locus_tag="VIN32_04485"
     CDS             complement(14404..16581)
                     /locus_tag="VIN32_04485"
                     /inference="COORDINATES: protein motif:HMM:NF024634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEY8921870.1"
                     /translation="MEVGIPFYPLIVLPAAAAIALFWRRAGRHLAVLRAGRPIDRLDQ
                     PLRRIWGVIVYVIAQRRLLNELGTGLMHAFIFWGFLVLLVTTGNYFTNGLVEAILRWP
                     LGGIAWLAAVAIANIFIGLVVGALVYATWRRIVQRPARLALSRDAFIILAMILGVVVS
                     ETAGDALRFVARPDDPARVAAILAGPLSLALAPIGPDAAATGYGIFAWLHIGFVLAFG
                     TYLPYSKHLHIITSEPNVYLRNLEPRGALRKMDLEAEPAAGEEVVFGAKGLKDLTWRH
                     LLDGLSCTECGRCMEFCPASMTGKTLSPKHFMEGLRDQIVAAETALAAAAGAQRAAKG
                     GAAGGVQASDASLSLARERAAEALALPLVDHAIPEEAVWQCTTCGWCVEGCPVLIEHV
                     DSIVEIRRNLVLEESRFPKELNAAFRNMETAGNPWGQPKSARLDWARGMDVPVLGADG
                     GSSEAGAAAHRGVLYWVGCAGAFDDRNRKVVRAMAALLKQADVPFAVLGTRETCTGDP
                     ARRAGNEYLFQMLAEENVATLTAAHDEHSVRTIVASCPHCFNTIRNEYPQFGLSGIEV
                     IHHTQLLDRLVADGRLTPDRSNEAVVAYHDACYLGRYNDVYDEGRRVVEAVPGQSVVE
                     MEQHHRKGLCCGAGGARMWMEEREGQRINHRRTEQALATSPTAIATACPFCLVMLRDG
                     TTDLDRADVAVRDVAELLADATGAWSTGALDRSLSRGFRRIWP"
     gene            complement(16741..17721)
                     /gene="moaCB"
                     /locus_tag="VIN32_04490"
     CDS             complement(16741..17721)
                     /gene="moaCB"
                     /locus_tag="VIN32_04490"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011210852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional molybdenum cofactor biosynthesis
                     protein MoaC/MoaB"
                     /protein_id="HEY8921871.1"
                     /translation="MKASHLGSGGEPRMVDVGDKPVTARRAVAASTVRMRPEVLGTLL
                     DASGPKGDAFVVARLAGIAAAKRTSELIPLCHTIPLDRVEVELTPDREAGTVTIQAEA
                     RATARTGVEMEALTAASVAALTLYDMAKALQRDIVIERVELLAKEGGRSGSWSKEGGV
                     APVKPVPAVHEAVVITCSTRAAAGERSDESGPAVVAALRAAGFEVAPEPILLPDDEDL
                     IASTLARLADAGTRLIVTSGGTGLTPGDRTPAATRRVVDREIPGLAELMRAAGLASTP
                     MASLSRGVVGARGTALIVNLPGSPRGAIESLQAVLPVLRHALEQLAGGDH"
     gene            complement(17718..>18085)
                     /locus_tag="VIN32_04495"
     CDS             complement(17718..>18085)
                     /locus_tag="VIN32_04495"
                     /inference="COORDINATES: protein motif:HMM:NF015419.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin molybdenumtransferase MoeA"
                     /protein_id="HEY8921872.1"
                     /translation="FGSIGDRPVIGLPGNPVSALVTFELFVRVLLRGMLGLTGDGRLH
                     LPARLDTTIPKDRDRRAYLRVVIRSEPDGWRASPAGGQGSAQLRSLAAANALLVVPEG
                     EAAAEAGTRYDAVLLRGIS"
BASE COUNT         2772 a         6361 c         6098 g         2854 t
ORIGIN      
        1 gcatagcccg cgccggcgct gcgggctacg tcgagggcca ggtcggtcag ctcctgcatc
       61 gcttcctcat tccaccggct tgccgatcac cggctgcagc gagaccgcgc tcgccggcag
      121 ctgcaccgat gttgcctgcc ggatctggat cgtggccttg atgaacagct ccagctggga
      181 aatgatccca cctgggcgct ccattgcgcc aagcaacccg tcgggggacc cgatcgcgcg
      241 gagcgtgaac tgcgccccga cctcgacccc atcgagctcg atggcacgcg gaccctcaat
      301 cgccaccgag cgagccgtga tccgtacgcc gtcgaccgcg atggcctcgg cgcctgcgtt
      361 gcggagctcg ttgatgaggt cgtttaccgc aatcgcgtcg aggtcgccgt cgacgtccat
      421 cacgattccc tgcccgtcca ccgcggccag tccgccgaag gcggtgatgc gtcgcagatc
      481 ctcgcggctg acctgcaggg cgctgtcgcc ctgcggcccc gacacctcgt attgccgcag
      541 ctgctcgcgg atgtcggcca ggcccgagcg cagctcacga ttgcgtgcgg tgagggtctc
      601 gatcagggtc gacagctccg tggccgacag gcggctgaca gcgatagggc gggcctggga
      661 gttcagctgg cccacaccca gcaggccgat cagcatggcc accgcgaaga gggtggcctg
      721 tgcaacccgg ctcttcatca gggcaacccc tcggtcggga cggcccagcg gaaccgcgtg
      781 ttgcccacga aggcggccag ctcaaccgca tccgcccgct gggtggcgaa ctcgagcccg
      841 aagacgtcga ttcgctgcgc cacgcggccc aggaaggcag ggttcgtttg gaaccggtcg
      901 agcaggccct cggcaccgat cgcctcgatc ctgaaggggg gagacaggaa ggcggtgttg
      961 acgagcacgg acgctcccac gccgtagatc gacgaggtcg cgacgaggcg ctcgccattg
     1021 atcgtgatcg cctctgcgcc cgaggcccac agcgcgacca cgatgtcgcg caggtcgtcc
     1081 accagcacga tgtagttggc tgggttctcg ccgtcgggga tcacgcgctt ggagtcggct
     1141 atctcgatga tcacgcctgg acctcgcagt ggcgccaggc cgacggccag tcgcacctcc
     1201 tcgagacgcc ggttcacgtc cgccagcgcc gacgtcgagg tggtcgacgc cttctgaaag
     1261 tccagcacct gggcctccgc tgcggcgagc tggccccgga ggtcctcctg ctccgcctcg
     1321 agcttggaga cctggtctgc gaggacctgc tggacggagg tggcgaactg gacgcgctcc
     1381 ggcgacccgt tccattgcgc atagccgacg aagccgatca ccgccagcgc acctgcgatg
     1441 ctcaccgccc attgggggct gaccagcagt cgccgtcgtc gcgcggtcac ggcgaggcac
     1501 gccggcggcg aacacgcacg agcgcaacca cgacccccag cgcaagtccc gcaaggacca
     1561 caaccgggat ccagcctgta tcgccatccg attgggcggg ctccggcacg gacgaagccg
     1621 cgggggccgc gctctcccct gcgggagcgc ccggctgagg cggcttgtca acgttggagg
     1681 gcaggatcgg tgcgggctcg acggcggtgg gcgctgccac gccgaacgag gcgaagtagg
     1741 tgtcgtacag ctgatcggcc ttgacgcccg tgcctgcttc gagcgcctcg gcatcgcctg
     1801 cgccgtcccg ccacgcggcg gcgatggccg cgatcgcccg gcggccgtag gtatcgatga
     1861 tcatcgcgac catggtggca ccctcgtcgt aggccaggcg cgcggcctga tcgccgatgg
     1921 ggaaagactc ggtgatgccg tcgaaggcaa gcatgccacg cgcgtccgcg gccgatctga
     1981 ccccaccctc gtgcccgctc gccgactgct gctcggacca taccgccagg ccctcgttga
     2041 accacttggc cggttggtgg aacgcattcg cggtcgcatc ctggaagacg acgtgcgtca
     2101 cctcgtgggt catggtcgtc tcgaggtagc tgctcgatcc gccttgcagc cacatgaaga
     2161 tcgtgcgcag ttcgggaaac gtcgcggcgc cggtccattc acgagctccg ggtcccaggg
     2221 cgccgaagaa gtcgtcgtgc gagtcgtaga cgaatatgtc gatcgggccg gccaggtcgt
     2281 gacccagcaa tgcctccgcc gcggcggcgg cgtccgcgga gacggagcca aaatggcgag
     2341 cctgcgcctc cgctccgccg taccagtgca ccgtcgccgc accgatctgc gctgagtgcc
     2401 agtcgagccc cggtcgatcg tcgtcataga ggagtgtctt ctcgtcgctg agggtcacct
     2461 tgtcgccgac ggtggcgcgc catcgatacg cgatcgacgt gttcggcgtg atgggctgat
     2521 tggccgtgtc cctgacaaac tccgcgctcg acggccctgg gtccaccggg accacgagtg
     2581 tggagtcgga gcccgcgaat cgcagcagca gcgcgagctg gtcgggcggg ccgctcggca
     2641 gctgcacggt gaagcgcatc tgccctccgt aggtggcgtc cgcggtcatg gagccgaagc
     2701 cagtgaatgc cgccgcggga agcgggacgg cgatcgtcag cgccagcacg atcgccgccg
     2761 cccggatggc tcgtcgcatc actgttccca tgagacccgg ccgaggtcca ggaggccggt
     2821 cgtcaggttg ccgaggccgc gcaggccggg gcgtgcgagc caccagccgg cgccgtaggc
     2881 ccacggaatg accggcacct cgctgtcaac gcgatcgtcg accgcgcggt aggcgacggc
     2941 ctggtcagcc tcgctcccgg cagcggatgc cgccgccagc agccgcacga attccggatc
     3001 tttccatccg ccgtagttgc tcgtggcatc gggcagcagg aggaggctgt agagcgcata
     3061 tggcgacggg tagtcggcaa tccaattgat cgtgaagacc tgcggtgggt cgaccaccag
     3121 cgctcgcagg tagtcccgga actccatcgt ctcgatcacg agcttcaccc ccagctcctt
     3181 ctcccagatc gccacggccg gcccgacccc aaggccggag ccgttgaccg tgatcgtgcc
     3241 cagccgcgcc cggtcggtgt agccggccgc atcgagaagg cttcgcgcct gctccgtatc
     3301 ggtcacctgg tcgtccttga gtccctccgg ccagagcgcc ggtggcacca ggctggacgc
     3361 gggagtccca ctgcttccct ccgcgttcgt gaccaggcgc ggccggtcca gcgcgagggc
     3421 gaaggctcgc cgcacccggg cgtcgttgaa cgggggccgc gtcgtgtcga agccgaagaa
     3481 ctggattccg agcgccgccg agcggtgcag cgacggcccg agatcgtggt cgtaggcaat
     3541 ccagcccgca tcgaacgagc ccacgctggt gaggtcgacc tcatcgttgg cgaaggcggt
     3601 cgccgagtcc ccggtcaggt tcgtgaccca gcggatctcg gcgatggggg gtggccccgc
     3661 gacgtaccta tcgttggcac gcaggaccag gtcgccggca tcggtcccac gcgcaacgta
     3721 tggcccggag ccgatgaagc tgtcgcttga ctgccagctg tcgttggacc cggatgcgtt
     3781 gcgcgggacg acgaaggccg tcggggtcgc cgtgatcgcg gtgaagtagg cggcgggatg
     3841 cctgagccgc acggtcagcg tccgtgcatc gggtgcatcg atggcaacgc catcctccgg
     3901 accgccggca agccgctcgg cggcgccttc gatgatgctc agcacgtccg gggcggtcga
     3961 gtgggtggcc ggatcgagga tcctgagcca ggaacggcgc acgtcgctgg cgtcgagcgg
     4021 cgtgccgtca ctgaaggtga gcccggatcg catgtggaac gtatacgtgc gaccgtcgcc
     4081 gctcacggtc cacgattcgg cgagcgacgc atatggctta ccatcctcgt caaggcgcgt
     4141 cagcccggcg tacagctgaa gcaggaactg gacatcgccg gcgctgtcga tgaaggccgg
     4201 atcgagggtg gcgggctcgc cgcccacgta gcgcaatggc ggctcgactg cgaaggcggg
     4261 gtgcgccggc agcgacgagg tgaaggccag gaggatggct gcgaagcctg cgacgaggcg
     4321 cggcagccgg ccccgcgagg tcaggcggcg catgcggggg ctagtatacg ggcccgtgca
     4381 gccgacccat gaccaccgct ttcggccgca gcccgcgggc agtctcacgc tcagacagtt
     4441 cgtcgagtcg cttcgccgat ggacgcccga gatgcggcag gttgcgcaga tcgcgttgac
     4501 tcgctcggag actgagcggc agcaatacct ccaggcgttt cacgcgcgcc accatgaccg
     4561 cgagcccaag ccgatcgact ccttcgtccg ctgggcatac tcggaccccc ggctcgatca
     4621 cgttcccgcg ctgcttgccg agatcgagcg aggcgacgac gagtacgagg aggctcttga
     4681 ccacgaggcc gatgtgctgg tgcgcacccg cgcacacggc aagaccccgg ccgagcgcgt
     4741 tcacgactac ctggccctcc tggggatgga gggcagtgag tcggtgcgta ccgccatcgg
     4801 aagcctgctg ccacccgcca cctccgacgc cgccgtggtg gcaaccgtgc gccagatgcg
     4861 ccgccggtgg ttggacctgt tcggcgacga tccgttcttc gccgaagaat agatagatgg
     4921 acgtcagcct tgccctcgcc ttcgccgcag gcctgctcag cttcgtgtct ccctgcgtcc
     4981 tggcgctcgt cccggtctac ctcgctttcc ttggcgaatc ggccacggta gcgggatccg
     5041 gaacggcgac cctcaactgg cgcggcccgg tcttcaccca ggcgctgctg ttcgtggccg
     5101 gcttcgcggt catcttcgtg ctgctcggca catcggtcgg gctcttcggc agcttcctgt
     5161 tccggatcga tgccgctcgt cagctggccg gggctgtcgt catcgggatc gggctgctca
     5221 ccaccggcat cttcggaccg gtgctggatc gcttcatgag gttcggcgta cggacggatc
     5281 gtctgcccga tgcccgagcg gccagggccc tgacactggg agcgctcgtc gccatcggct
     5341 ggacaccctg catcggcccc gtcctaggcg cgatcctgac gatgggcgcc tcgagccggg
     5401 atgcctgggt cgctgcgctg ctgttgattg cctattccgc cgggctggcc gtcccattcc
     5461 tggccgcagc cctggcgctc ccgcggatgc agccactgct caatgccctg cgccgccacc
     5521 atcgggtggt tcaggtcgtc tccggcctct tcatcatggt gatcggcgtg ctgatcctga
     5581 cgaacgcctt cgcccgaatg gcagggctat tcaccttctt catctgacga ggtgaaccgg
     5641 tgcatcccgc cctgtgcccg cgctcgatcg gctcggcggg gtcgtaactc taacggagtg
     5701 ggtaccgcga ccgcccagta ggacgcggaa cccaccccga atgagagagc ggcgtcagct
     5761 gccggtcggc aggatgctct gcacgacgct ctgcacgacg ctctgcagcg gcgcggtgct
     5821 attcgacggc gtgctactcc cgccaccgcc gttgagcagg aaccaccaga taaggaagcc
     5881 gatgatgatc actgccagga tcaggccgac gatcatgccg gctcccattc cttcgtccga
     5941 gctcccgccc ggcacgttca cgttggtctg actcatgggc tttgactcct ccttgtgagg
     6001 ggcgagccat gtcgacccac ccgagactgt ccgatggtgc cgtggagaac gttgcagtag
     6061 cgtatcaccc gtccagcggc tcatgccgcg cgggagacag tcggaaatgg cgcccccgga
     6121 gggaatcgaa cccccgacgc aggccttagg acggcctcgc tctatccgct gagctacggg
     6181 ggcggcgcgt cgaagatcct atcatcgggc catggcagtg ccgtcgcgcg tgactctcta
     6241 tgagcgcgat ggctgccacc tttgcgacga tgctcgcgtc ctcctcgacg agatgctggg
     6301 gagccacggc tacgcacgcg ttgacatcga cgccgatgac gcgctcgtgc tgcgatacgg
     6361 cttccgcgtc ccggtcatcg ggctcgatgg cgtcgatcgt ctggaggcgc cgatcaccgc
     6421 ccccgacctt cgcgcgctcc tggccgagcc ggatgcctga tcggtgtcga gccttcacga
     6481 aggctcgctc ctgacctgga atcccttcgc ggtggcctcg atctggatcg ggagctcgcc
     6541 ggggtcgtgg gtgaagtagc accaccatcc ctcggcggcg gcacgcgtca ccagctcatt
     6601 tcggatctcg acggcctgga tcgggtagtc gtcgaaggcg ctcgtccagc gcaccgggat
     6661 ctgccagcgc gtggggatca gatcgcccca gaagatcgca cgctcccccg gatcctcgtc
     6721 gcggccctca ctgcgcgtcc cgagcagcac cgcctgcgat ccgcgggtgt gtccgccggt
     6781 cggcagcacg ctcacgccgg ccaccagctc gacctcgccc tgcacctcgg ccagctggcc
     6841 ggcgccgcgc acaaggtcga gctgctcgcg ctccatgatc cccgccaggc gcagctcgtc
     6901 tccgtgcgcg gcctctgact cgtcacgctg caccacgtaa cgcgcccctg gaaagttcgg
     6961 attgccatcc acatccacca tcccgccagc gtggtcgtaa tgcagatggc tgacgaccac
     7021 gaagtcgatg ctcgctgggt cgacgttcac ggcccgcagc gctgccgccg gatcgccgcc
     7081 ctcgactccc ttgatgtcgc ggtccttgtc ggtcatccgc accccggtgc cggtctctat
     7141 caggacgcgc ttgccgccag cctcgaccag cagcaggttg agccggcaca gcacgcgtcc
     7201 acgctcatcc gcctcgacca gccgcgacca gaggggtcga gggacgacgc cgaagaaacc
     7261 gccggcatcg ggccgaaacc agcccgcgcg cacgacgcgg acgcggacat cgccggcgta
     7321 cgtgcgtgcc tggcgggcag gcgtgatggc gaagagtggc tcggttacgt cgaactccgg
     7381 ctgcaggggc cctggggctc agcgcgtgat gcgacctcgc tcgaagtccc gatggtacag
     7441 gctcgccgtc gggtctcgct gtccgcggta cttgctgccg atgctcgcat cgccgtaggg
     7501 cacctcggcc gggctcgaga ggcgctggaa gctgatctgg ccgatcttca tgccgtcgta
     7561 cagcgtgatc ggcaggttcg ccacgttcga cagctccagg gtcaggttcc catcccagcc
     7621 gggatccacg tagcccgcgg tcgagtggat gagcaggccc aggcgcccga ggctcgactt
     7681 gccctccagc ctggccacga ggtcgttggg caacccaacg cgctcaaagg tggagccgag
     7741 cacgaactca ccggggtgca ggatgaacgg atcgtcgccc ccgatctcga ccagctcggt
     7801 cagctccggc tgatcggccc gaacgtcgat gaacggatat cggctgttgc ggaagacgcg
     7861 aaagcgtcgg tccaggtgca ggtcgacgct cgagggctgc accgcctcgg ggatgaacgg
     7921 gtcgatcacg atccggccgg cggtgatctc ggcccggatg tctcggtcgg agaggaccac
     7981 tagctgtccg ctggcgtgcg ctgcttcatc agccggtcga cctcccgctt cagggtcgag
     8041 atgtcctcat agctgtgata gaccgatgcg aagcggatgt aggcaagctg gtcgatgtcg
     8101 cgcagcgctt cggtggccag gcgaccgatc tccttgctgg tgacctcgga ctcgcctcgt
     8161 gcgcgcaggc caccctcgat gtcgtcgacc gcctgctcaa cgcgggccag ggtcaccgga
     8221 cgcttctcga gcgcccgcat caggccggcc tggagcttgc cccgatcgaa ttcctggcgg
     8281 ctcccgtcgc gcttgaggac ggccaggcgg gcgccctcgg ggcgctcgta ggtcgtgaag
     8341 cgatacgagc attcctcgca ttcccgccgg cggcggatcg tgcctccccc ttcaaggtca
     8401 cgtgagtcga tgacccgcgt gctggcctcg ttgcagcgtg gacagagcat gcaggctccc
     8461 cgtacggact acaccagtgg tggtaccgtc ccgaacgata ccacaagatg tgccgtcccc
     8521 tgcccgcttt ctgcggcgga tcgggcccta gcgggcgcgc ccgagccggg ccccgacagt
     8581 ggcctcggcg ggcatccccg gctcccaggt caggagcgtg tccacgacct cctgcatgcg
     8641 cagccgagcg tgcaggatgt tcaggtggcc gcgcttccag tagatgagcg gctcgtcgtc
     8701 atcgggaggc cggtacggca ggcgggtgat cccacatccc cgctccgcag tgacgatccc
     8761 gcgcagtgcg cgctcgaggc tcccctcgta ctcggcgtgc agcgcgtgca gctcctcggg
     8821 tgccagcatg tcgcgcagcg agtcagcggc gaggtcgagc ttctcctcac cggcctgcag
     8881 gtcgaccagg acctcgtccg gatccgcatc acggcgccgc agcgcatcga ccaggtcgcc
     8941 cagcatcgcg agctccaggt cgaccatggt ggtcacctcg gcgaggccgt tccgatacgc
     9001 ctgcagctcc cgaccgtgcg cctttgggcg cttcggcccg cgtctgcgaa gcagtcccac
     9061 ggtatcagcg tccctttcga cgccgaatct cggcggtcag cttcgggacg atggcgaaca
     9121 ggtcccccac cacgaacagg tcggcgaatt cgccgatggg cgcgtcggga tccttgttga
     9181 tggccagcac gttctcggcg gtttgcatgc cgacccgatg ctggatcgcg ccactgatcc
     9241 cgcacccaac gtacagggcg ggcttgacga ccttgccggt ctggccgatc tggagctgat
     9301 aggggaccca gcccgcgtcg gccgccgcgc ggctggcgcc aaccgccccg ccgagcgcgt
     9361 ctgccagctc ctgcagcggt gcgaagccat ctgcgctgcc gacaccccgc cctccggcga
     9421 cgatgacgct cgcttcctcg agattcgcga ctgcggcctc ggcaggatgc gactcgacga
     9481 tgctggccga ccccagcgct ccagcctcgg acgccggagc gggctcgacc gcggccttcc
     9541 cgctcccgcc ttcgagtggg gagaaggtgc ccggcagaac gagcacgatc gccggctgct
     9601 cggcctcgtc ggttggttcg ctgacggtga tgacggttcc ctggagcgtg gcctgctcgg
     9661 tcctcacgcg gcccccctcg atgcgcaccg cgcgaacggg cccgaaggcc ggtaccccga
     9721 gcaggccgac caatgcggcc gcgagatccc ggccgttcgg tgtcgccggg gccaggagcg
     9781 cgatcgggcc tatgccacga accgctgccg cggcagcagc gccatgcgtg atggcgggcg
     9841 aggggtcatc gcccaggatg acgacgcgcg acgccccagc ggagccgagc gccgcagcgt
     9901 cggccgcgcc gggaccgtag gcgagggcaa ccgccgaccc cttgctctgt cctgcgagct
     9961 cggctgcacc ggcggccagc tcgagcgcgg cgtgcgttgg cgagccaccg ctgaattcgg
    10021 cgaggaaggc gacgtcgctg ctcatcagat gagcttcctg gaggcgagcc aatcggcgat
    10081 gcgcgtcacg gcctcgtcag cgctcacgtt ctcgatccgc tcggtcggcg gctttggctc
    10141 tggcttgcgt gtctcgacga cgcgggtcag tgccgacccg tgcaggtcgt caggcgacag
    10201 gccgatgtca gcgaggctcc atgtctccat cggcttgcgc ttggcagcca tgatcccctt
    10261 cagcgacgcg tagcgcggct cgcccacctg gtccgtcacg ctggcgacca ccgggagcgg
    10321 cgcctcgacg atgtcgaacc cgtcggccgt caggcgccgg acgcgcgcag ccgaaccctc
    10381 gatgcgcagc tccgacccgt acgagagcag gggcactccg cgcatggcgg ccactgcacc
    10441 cgggaccagt gatcctcggg cgtcgtcgga cgccatgccc atcagagcga catccggcgc
    10501 caacttgtcg agcgcagcgg caagcacgcg tgcggtggcc catgcatcgg ctccggcgag
    10561 ggccggatcg gtcacgaggg tcgcgtcgtc gccacccatg gcgagcgcac ggcgcagcgc
    10621 ctccgcagcc tgctcggggc ccatcgacag gaagccgacg gactcgaccg cgcccgactc
    10681 ctgcagtcgc aacgcctgct cgatggcgta ttcatccagc ggattgatga ccgcttctgc
    10741 cgcagctcgg tccagccgca ggtcggcggt gtagcgcttc tcggcggtgg tggcgggcac
    10801 ctgcttgatg cagaccacga tcttcaggct gatggtcgga actcccctgc tacctggaag
    10861 gccgggatac gcccgcccgt cgaaggagct ggcgcgccac gatcatgcgc tggatctgct
    10921 gcgtcccctc gtacagctgg gtgatcttcg cgtcgcgcat catgcgttcg acagggtact
    10981 cggtcacata gccataaccg cccaggacct gcaccgcgtc cgtcgtcacg cgcatcgcca
    11041 tgtcgcccgc gatcagcttg gccgagctgg tccagtacgc caggtcggga gcgttggcgt
    11101 ccaccgccgc ggcggcggcg taggtcagct gcctggccgc ctcgaccgcg ctggccatgt
    11161 ccgcgagcat gaactggatc ccctgcatgt cggcgatcct ctcgccgaac tgctcccgtt
    11221 ggcccgcata gccgactgcg aagtcgaggg cgccctgcgc gatgccgacc gcctgtgcgg
    11281 cgatcgagag gcgcgagcgg tcgagggtgg acatcgccac ggcgaacccg tctccctcgg
    11341 cgccgagcag gttggcggcg ggaacccgga cgtcgtcgaa gtgcagctcg gccgtgggtg
    11401 agccacgaat ccccatcttg tgctccagac gcggcgccga gaagccggga ctatcggttt
    11461 ccaccacgaa ggcgctcagc cccttgcgcg ccccgacgtc ggcatcggta cgggcgatga
    11521 tcgtgacgag gttggcgatc gagccatggg tgatgaagcg cttgctgcca ttgaggaggt
    11581 agtcgtcgcc gtcgcgcctg gcacgcatcc ggatgccggc cacgtcgctc ccggcgccgg
    11641 cctcggtcag gccgaaggcc gccatccact cgccgctcgc caggcgcggg atgtagcgcg
    11701 cctgctgctc ctcgctgccg gcaatcagga tcggcagggc accgagcttc tgcacgatca
    11761 cgatcaggct ggtcgtcgcg tcggcgcgtg cgatctcctc caccacgatt gcctgcgaga
    11821 tgtcgtcgag ttcggttccc ccgaagcgcg ccggaaaggc gatgccgagc aggtcctgct
    11881 tcgccagcag ctccttgagg tcccacggaa actccgacgt ggcgtcgatc tcggccgcgc
    11941 gcggcgcgac gcgttcgtcg gcgagctcac gcaccacggc gcgcagctca tcgtgatcgg
    12001 cggtgggtcg ataggtcacc cgatgaccgg tccctggacc tcgacctggt cgccgttgat
    12061 ccgcgcgcga tatgcgacct ggcgggtggc ggccggaccg tgcctgacgg cgccgtcact
    12121 cagccggaac gccgacccgt gccacgggca gatgacggtg tagccgtcca gcgtgccctg
    12181 cgagaggggg cctcccgcgt gcgagcaggt ctccttgaga gcgaagacga ggtcgccgcg
    12241 ccgcaccagc acgagtgcgg tcggcccgag catggccttg gtgggcgtcg cctcggcgag
    12301 atccgcttcc ggcaggatgg cggcgtactc cttcgccttc tttccgcccg aaaaggcatt
    12361 gtgattgacc atgtagccgt acttgaagac gatatgaccg ccgatgaagg cgccgacact
    12421 gaggatggcg aaccctgcca cgatgaggac gccggcgagc aggtccgcat cggccagcag
    12481 ccccaggaat gcgccaatca acaccgtggt cgccaggatg ttgatcaggc cgtggagccc
    12541 gaccacgtgc cgctcgtcgc cggtggcggt gtccttgaag tcggtcaggc cgctgacgat
    12601 cgccgacagg ccggccagcc aggtgagcgc cagcacccac aggatcgcca cccgcaggtc
    12661 gcccaccccc agccagctca gcacctgcag caggacggcg gcggtcgagc cgccaaccac
    12721 cacgtcgacc accaccggat gcagcgaatg gccgagccac gacccgttca gcaagtcctg
    12781 cagcagcttg ccggggcgcc cgagccggcg gtagatggcg tccatcaccc cggcagtgcc
    12841 gttccacagg cgtgcccagg gcctcgccca tcgctcaagc atctcgattc aggccctcag
    12901 cccgtgacct cgaccgccca ttcgcggaag accgcaaggt agcggtcgat ctcgtcatcc
    12961 gtgtgttgga cggagagtgt ccactggtcg tcgggggcgg gtgggatgag gatcccgcgg
    13021 ttggccagga accagaacga ggacgcccac agctcgtcat cgaggccgat gaagtcgcga
    13081 tagccggtga ctcgcttcgg cgcgaacatc acgatcccct tcggcccgac gttgatggcg
    13141 tagcccggaa gcccgtactg gtcgacgatc tcctggctgc cggccgtcaa ccgatccccc
    13201 atcgcctgaa ggcgcgggta gacgtcccga gtgagcaccc tggtgagcac ggcaagcgcc
    13261 gccgccatca ccagcggcga gccattcatc gtgcccatgt gcgcgacccg ggaggtgccg
    13321 ccaaatgacg agtgactcag ctcggcctcg atcagcggtg ccctggcgcc accctcgatc
    13381 acccgcatca cctcctcgcg cccgccgaac gcaccgatgg gcgtgccgcc gccgatgctc
    13441 ttggcaaggc agaagaggtc cggctgaacg tcgaagtact ccgtagcgcc accgtaggcg
    13501 atgacgacgc cggtcttgac ctcgtcgaag atgaggacgg cgccgtgggc ggtcgccagc
    13561 tctcgcagcc gctccaggaa gcccggttca ggcgggacga cgccgatgtt gaactggacc
    13621 gcctcgacga tgatcgccgc gatctcgccg cgatggcgct cgaggatccg ctccacgatc
    13681 tcaggctggt tgaacggcgc aatgatcgtc tcggcgatcc ggctgcgcgg gatgccggca
    13741 ctggtcggga cgctgttcgg cgaatcgacg tcgccgatca tgtcgcgcgg cggctggatg
    13801 ctgatcagga cgtcgtcatg gtggccgtgg tatccgccct cgaacttgat gatcttctcg
    13861 cggccggtga agccgcgcgc cacccgaatg gcgtccatcg tcgcctcggt gccggagttg
    13921 ctgaagcgaa ccagatcggc ccggaagcga cgcttcagct cctcggcaac caggatctgc
    13981 tcctcgatcg ggaaggtgaa ggcggtgccg atcgctgcgc gtcgctggat cgcctccacg
    14041 aactctgggt gagagtggcc ggcggccagc actccgaggg ccatgtcaaa gtcgaggtat
    14101 tcgttgccgt cggcgtccca catccgcgac ccgcggccct cgctgatgaa cagcgggtag
    14161 gggtcgtagg tctggaacga gctggccacg cccagcggca tcgactcgag cgcgcgcttg
    14221 tggagctcgg cgctgcgcgg cgtgcgcccc cgataggctg cctcttcgcg cgccacgatc
    14281 gccttgaccc gggcgcgatc gacctcccgg cgttcgatta cggccacagg tcctccttct
    14341 tgctcgccga tcctagccga tcagcgccgc gtgcaagcgc cgggctacgc cgcgcccggg
    14401 cagctacggc cagatccttc ggaatcctcg gctcagcgac cggtccagcg ccccggtcga
    14461 ccaggcgccg gtcgcgtctg ccagcagctc cgcgacgtcg cggaccgcaa cgtcggcacg
    14521 gtcgaggtcg gtggtgccgt cgcgaagcat gaccaggcag aaagggcagg cggtggcgat
    14581 cgccgtcggt gaggttgcga gtgcctgctc ggtgcggcgg tggttgatcc gctgtccctc
    14641 gcgctcctcc atccacatac gggcgccgcc cgcgccgcag catagcccct tgcggtgatg
    14701 ctgctccatc tcgaccacgg actggcctgg gaccgcctcg accacgcgcc tcccctcgtc
    14761 atagacgtcg ttgtagcggc ccaggtagca cgcgtcgtga tatgccacga ctgcctcgtt
    14821 gctgcggtcg ggggtgagcc tgccgtcggc gaccagcctg tcgagcagct gagtgtggtg
    14881 gatcacctcg atcccggaga gtccaaactg cgggtactcg ttccggatcg tgttgaagca
    14941 atgcgggcag ctggccacga tggtccgcac gctgtgctcg tcgtgggccg ccgtgagcgt
    15001 cgcgacgttc tcctccgcga gcatctggaa caggtattcg ttgccggctc gacgagcggg
    15061 atcgccggtg cacgtctcgc gcgtcccgag caccgcgaag gggacatcgg cctgcttcag
    15121 gagcgctgcc atggctcgca ccaccttgcg gttgcggtcg tcgaaggcgc ccgcgcagcc
    15181 gacccagtag aggactccgc gatgcgccgc ggcgcctgcc tccgatgagc cgccatctgc
    15241 cccgagcacg ggcacgtcca tccccctggc ccagtcgagt cgggcgctct tcggctgtcc
    15301 ccacgggttg ccggccgtct ccatgttgcg gaacgccgcg ttcagctcct tggggaatcg
    15361 gctctcctcg agcaccaggt tgcggcgaat ctcgacgatg ctgtcgacgt gctcgatcag
    15421 gaccgggcag ccctccacgc accagccgca cgtggtgcat tgccagaccg cctcctccgg
    15481 aatggcgtga tcaacgagcg gtagcgcaag tgcctcggcc gctcgctcgc gagccaggct
    15541 cagcgaggca tcggaagcct gcactccccc ggcagcgccg ccctttgccg cacgctgcgc
    15601 cccggcggct gccgccagcg cggtctccgc ggccacgatc tggtcgcgca ggccctccat
    15661 gaagtgcttc ggggagagcg tcttgccggt catcgaggcg gggcagaact ccatgcagcg
    15721 gccgcactcg gtgcacgaca gcccgtccag caggtggcgc caggtgaggt ccttgagacc
    15781 cttggcgccg aagaccacct cctcgcccgc tgccggctct gcctccaggt ccatcttgcg
    15841 cagggcgcca cgcggctcca ggttgcgcag gtagacattc ggctcgctgg tgatgatgtg
    15901 caggtgcttg ctgtacggca ggtacgtgcc gaaggccaac acgaatccga tgtgcagcca
    15961 cgcgaagatc ccatagccgg tggcggccgc gtcggggccg atcggggcga gggccagtga
    16021 gaggggacct gccaggattg ctgccacgcg cgccgggtcg tcggggcgcg cgacgaatcg
    16081 cagggcgtcg cccgccgtct cgctgacgac gacccccagg atcattgcca agatgatgaa
    16141 ggcgtcccgg ctcagggcca ggcgggcggg ccgttgcacg atacggcgcc aggtcgcata
    16201 gaccagcgcg ccaaccacca ggccgatgaa gatgttggcg atggctaccg cagccagcca
    16261 ggcaatgccg ccgagcggcc agcgcagaat cgcctccacc aggccgttgg tgaagtagtt
    16321 cccggtcgtg accagcagga caaggaagcc ccagaagatg aaggcgtgca tcagcccggt
    16381 gccaagctcg ttgaggagcc gccgctgggc gatgacgtaa acgatcacgc cccagatccg
    16441 acgcagcggc tgatcgaggc ggtcgattgg ccggccggca cgcaggacag ccaggtggcg
    16501 gccggcgcga cgccagaaga gcgcaatcgc ggctgcggcc ggcagcacga tgagggggta
    16561 gaacggaatc ccgacctcca tgacggccat gctacccgcc actccaccgg tcccgatgcc
    16621 ggtgtggact agagacgacg gctatcggct cgaaccgtgt cgtccctatg ccgccgctgg
    16681 catatctcga cccgttggcg ccgcaatcga gtcgaaccga gtccgtgcag ccatcagcga
    16741 tcagtgatcg cccccggcta gctgctccag cgcatgccgg agcaccggca ggaccgcctg
    16801 caacgactcg atcgcacccc gtggcgagcc cggcaggttc acgatcaggg ccgtgccccg
    16861 ggccccgaca acaccgcgcg agagcgaggc catcggcgtc gaggcgaggc cggccgcgcg
    16921 catcagctcg gccaggcccg gaatctcgcg atccacgaca cgccgagtgg cggccggagt
    16981 tcgatcacct ggcgtcagcc cggtgccgcc gctggtgacg atcagccgcg tcccggcgtc
    17041 ggccagccgc gcaagggtcg aggcgatgag gtcttcgtca tcgggcagga ggatcggctc
    17101 gggcgccacc tcgaagccgg ctgcgcgaag cgcggcgacc acggccgggc cggattcgtc
    17161 gctgcgctcc ccggctgccg cacgcgtcga gcaggtgatg acgacagctt cgtgaacggc
    17221 tgggaccggc ttgactggcg ccacaccgcc ctccttcgac catgagccgc tcctgccccc
    17281 ctccttggcc agaagctcga cacgctcgat cacgatgtcg cgctgcagcg ccttggccat
    17341 gtcgtacagc gtcagcgccg cgacgctcgc cgcggtgagc gcctccatct cgaccccggt
    17401 gcgggcagtt gctcgtgcct ccgcctggat tgtcacggtc ccggcctcgc ggtcgggggt
    17461 cagctccacc tcgacccggt cgagggggat ggtgtgacag agcgggatca gctccgacgt
    17521 tcgcttcgcc gccgcgatcc ccgccagccg cgcaacgacg aacgcatccc cctttgggcc
    17581 actcgcgtct aggagcgtcc ccaggacctc ggggcgcatc cgcaccgtgg acgctgccac
    17641 ggcgcggcgg gcggtgaccg gcttgtcccc cacgtcgacc atccgcggct cgccgccgga
    17701 gcccaggtgc gatgccttca cgagattcct cgcagcagga cggcatcgta gcgagtgccg
    17761 gcctcggccg ccgcctcgcc ctccggcacg accagcaggg cattcgcggc ggcgagcgag
    17821 cgcagctggg cggatccctg cccaccggcg gggctggccc gccaaccatc aggctccgag
    17881 cgaatgacga cccgcaggta cgcccggcgg tctcggtcct tggggatcgt cgtgtcaagg
    17941 cgggccggga gatgcaggcg tccgtcgccc gtcagtccca gcatgccgcg cagcagcacc
    18001 cggacgaaga gctcgaacgt caccagggcg ctgaccgggt tcccgggcag gccgatcacc
    18061 ggtcgatccc caatcgatcc aaagg
//
LOCUS       DATCQR010000131         3404 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_54043, whole genome shotgun sequence.
ACCESSION   DATCQR010000131 DATCQR010000000
VERSION     DATCQR010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3404
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54043"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1894
                     /locus_tag="VIN32_04500"
     CDS             <1..1894
                     /locus_tag="VIN32_04500"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY8921873.1"
                     /translation="CQEGCPVNTNCRGYLMLAAEGRFEEGYILARDPNPVAAICGYVC
                     SAPCEKACRRADIDKPLSIRAMKRFLVDWHYGNNMPDNVVTASPTGKTVGVIGAGPAG
                     LTVAKELASYGHKVDIYEALPSGGGTCLIGVPAFRLPRDVIELDVNWVAKHGVDFHYN
                     VEIGRDITLDQLRERHDAIVVSAGCMYPVAMNIPGEELNGVIYGVDFLKRANLGEEQW
                     VGEHAVIVGGGYTAMDSARTALRIGAKTSTIVYRRGPEEMVVDEEEQHETRFEGVRFE
                     FFSSPVEVVEKDGKVAGITFQRTRLGPPDASGRRSPEPIPGSEFTISCDMVIPCTSQA
                     SDNNVLGEYGAKLNRHLGVTDTREFVPGGSVSSWRTPPLNGTTPVGPDGRKYNALQFE
                     LSRGNVVAEPDTFQTSVDGIFACGDFVTGPATIIEAAGRGRRTARAVDRWLLGVRGEE
                     LVELPVINRAVVTQALEHDMPDKYEAIPRQHIPMAPPELRRDFTSLVEVGYDTRSAIS
                     EGQRCLQCNHNINIDGPRCILCGLCADVCPEGVIYMIDKAQVGGDDPEIDLFQAWPRG
                     IAMIIDEERCIRCNLCVERCPTNCITMDRLELERLTPDGKVLLESYKDNVLGQIGQEA
                     GMAVQR"
     gene            1891..2625
                     /gene="extP"
                     /locus_tag="VIN32_04505"
     CDS             1891..2625
                     /gene="extP"
                     /locus_tag="VIN32_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenite/tellurite reduction operon b-type
                     cytochrome ExtP"
                     /protein_id="HEY8921874.1"
                     /translation="MTFPGPGDLMRVVRESSVWRSLFRQPYPTTSRTRALAVMNNVFL
                     HLHPVRVKKHAVRYTYTFCLGGVSFFLFLVLTVTGVYLMFFYVPSVTRAYQDILSIEN
                     SVAFGTFVRNMHRWGAHLMVLTVFLHMIRVFYHGAYKPPREFNWVIGVVLLFCTLWLS
                     FTGYLLPWDQIAFWAITVGTQMATYAPLISRESSFVLLGGVEVGQGTLIRFYVMHVIA
                     FPLIVAVLLVVHFWRIRKDGGVSGPV"
     gene            2642..3256
                     /locus_tag="VIN32_04510"
     CDS             2642..3256
                     /locus_tag="VIN32_04510"
                     /inference="COORDINATES: protein motif:HMM:NF012262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinol oxidoreductase"
                     /protein_id="HEY8921875.1"
                     /translation="MTIEKRPNATADKNAPTQAPHVPEVERDRGPKLPGPPGVPGTER
                     FRLLAYVKQESMVRVDKRPDETVLTWPHLLRAEFLMALLISVLLVVASVFIHAPLEEM
                     ANAGVTPHVAKAPWYFLGLQELLSYFNATVGGVLLPTLYLVGLAVIPYIDRSPYKAAR
                     DRKTAWVFFMSLMLGGLLITLIGSFFRGPGWNWVWPWDGIYFTL"
BASE COUNT          543 a         1167 c         1119 g          575 t
ORIGIN      
        1 atgccaggaa ggctgcccgg tcaacacgaa ctgccgcggc tacctgatgc ttgccgcgga
       61 ggggcgcttc gaggagggct acatcctggc gcgcgacccg aacccggtcg cggccatctg
      121 cggctacgtc tgcagcgcgc cgtgcgagaa ggcgtgtcgc cgcgcggaca tcgacaagcc
      181 gctctcgatt cgcgccatga agcgcttcct ggtggactgg cactacggca acaacatgcc
      241 ggacaacgtg gtcaccgcct cgccgaccgg gaagaccgtc ggggtcatcg gcgccggtcc
      301 ggcggggctg accgtcgcca aggagctggc cagctatggc cacaaggtcg acatctacga
      361 ggcgctgccg tccggtggcg gcacgtgcct catcggcgtg ccggccttcc ggcttccaag
      421 ggacgtcatc gagctcgacg tcaactgggt cgccaagcac ggcgtcgact tccattacaa
      481 cgtcgagatc gggcgcgaca tcactctcga ccagctgcgc gagcggcacg atgcaatcgt
      541 cgtctcggcc ggctgcatgt accccgtcgc catgaacatc cccggcgagg agctcaacgg
      601 cgtgatctat ggcgtcgact tcctgaagcg tgccaacctg ggcgaggagc agtgggtcgg
      661 cgagcacgcc gtgatcgtgg gaggcggcta tacggccatg gactcggcgc gcaccgcgct
      721 gcgcatcggt gccaagacca gcaccatcgt ctaccggcgc gggcctgagg agatggtggt
      781 cgacgaggaa gagcagcacg agacccgctt cgagggcgtc cgcttcgagt tcttctcctc
      841 accggtcgag gtcgtcgaga aggacggcaa ggtcgccggc atcaccttcc agcgaacccg
      901 cctcggtccg cctgacgcca gcggccgtcg cagcccggag ccgatccccg gcagcgagtt
      961 cacgatctcc tgcgacatgg tcatcccctg caccagccag gcctccgaca acaacgtgct
     1021 gggcgagtac ggcgccaagc tgaaccgcca cctgggggtg accgataccc gagagttcgt
     1081 gccgggcgga tcggtctcgt cgtggcggac gccgccgctg aacgggacca cgccggtcgg
     1141 cccggatggg aggaagtaca acgccctcca gttcgagctc agccgcggca acgtggtcgc
     1201 cgagcccgac accttccaga ccagcgtgga tgggatcttc gcctgcggcg acttcgtgac
     1261 cggccccgcc accatcatcg aggcggccgg gcgtggccgc cgcaccgctc gggcggtcga
     1321 ccgctggctg cttggcgtgc gcggcgagga gctggtcgag ctgccggtga tcaaccgcgc
     1381 ggtggtgacc caggcgctcg agcacgacat gccggacaag tacgaggcga tcccgcgcca
     1441 gcacatcccg atggcgccgc cggagctccg gcgcgacttc accagcctgg tggaggtggg
     1501 ctacgacacg cgcagcgcca tctccgaggg gcagcgctgc ctgcagtgca accacaacat
     1561 caacatcgac gggccccgct gtatcctgtg cgggctctgt gccgacgtct gccccgaggg
     1621 cgtgatctac atgatcgaca aggcgcaggt gggcggcgac gacccggaga tcgacctgtt
     1681 ccaggcgtgg ccgcgcggga tagccatgat catcgacgag gagcgctgca tccgctgcaa
     1741 cctctgcgtc gagcggtgtc cgaccaattg catcaccatg gaccgactcg agctcgagcg
     1801 gctcacgcct gacgggaagg tgctcttgga gagctacaag gacaacgtgc tgggtcagat
     1861 cggccaggag gcgggcatgg cggtccagcg atgaccttcc ccggcccggg cgacctgatg
     1921 cgggtcgtca gggagtcatc ggtctggcgc agcctcttcc gccagccgta tccgaccacc
     1981 agccggacgc gcgcgctggc ggtcatgaac aacgtcttcc tgcacctgca cccggtccgt
     2041 gtgaagaagc acgcggtgcg ctacacgtac acgttctgcc tcggcggggt gagcttcttc
     2101 ctgttcttgg tgctgaccgt gaccggcgtc tacctgatgt tcttctacgt gccgtcggtg
     2161 acccgcgcct accaggacat cctctcgatc gagaactcgg ttgccttcgg caccttcgtg
     2221 cgcaacatgc atcggtgggg cgcgcacctc atggtgctca cggtcttcct gcacatgatc
     2281 cgggtcttct accacggcgc ctacaagccg cctcgcgagt tcaactgggt gatcggggtc
     2341 gtgcttctct tctgcaccct gtggctcagc ttcacgggct acctgctgcc gtgggaccag
     2401 atcgccttct gggcgatcac ggtcggcacg cagatggcga cctacgcgcc cctcatcagc
     2461 cgcgagtcga gcttcgtgct gctgggcggg gtggaggtcg ggcaggggac cctgatccgc
     2521 ttctacgtca tgcacgtgat cgccttccca ttgatcgtcg ccgtcctgct ggtcgtccac
     2581 ttctggagga tccggaagga cggcggcgtc tcaggccccg tctgagaagc gaattgagcc
     2641 gatgacgatc gagaagcgac cgaacgccac cgccgacaag aacgccccga cccaggcgcc
     2701 gcacgtcccg gaggtcgagc gcgaccgcgg ccccaagctc cccggaccgc ccggcgtgcc
     2761 gggcacggag cgcttccgcc tgcttgccta cgtcaagcag gagtcgatgg tgcgcgtcga
     2821 caagcggcct gacgagaccg tgctgacctg gccccacctg ctccgggccg agttcctgat
     2881 ggcgctgctg atcagcgtcc tgctggtcgt ggcctcggtc ttcattcacg cgccgctgga
     2941 ggagatggcg aacgccggcg tcacgccgca cgtcgccaag gcgccctggt acttcctggg
     3001 cctgcaggag ctgctctcgt acttcaacgc caccgtcggc ggggtgctgc tgccgacgct
     3061 ctacctggtc gggctggcgg tgatcccgta catcgaccgc tccccgtaca aggcggcccg
     3121 ggaccgcaag acggcctggg tctttttcat gtcgctgatg ctgggtggcc tgctcatcac
     3181 cctcatcggc tcattcttcc gcggcccggg ctggaactgg gtctggccgt gggatgggat
     3241 ctacttcacc ctatgagcca cctgcccacc tacggcgaag ttcccggcgc tccggtcgga
     3301 gagatgagcc ggcgcagctt catgcgccgc atcttctggg tgggaaccgg catcctctca
     3361 atcgagttcc tggccggctc cgtcaacttc ctgtggccga acgt
//
LOCUS       DATCQR010000132         2395 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_116556, whole genome shotgun sequence.
ACCESSION   DATCQR010000132 DATCQR010000000
VERSION     DATCQR010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116556"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(51..1268)
                     /gene="ispF"
                     /locus_tag="VIN32_04515"
     CDS             complement(51..1268)
                     /gene="ispF"
                     /locus_tag="VIN32_04515"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF013308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HEY8921876.1"
                     /translation="MPQPAGEPAVLIGAVLVAAGSSRRMGSDKLWIEFSGRPAWRWAL
                     DTLLAVPGMSWVVVVAPPDALERFRDAIPEAARGRAEVVAGGEERADSVLAGLAALTR
                     AGFPEDAAVLIHDAARPAASSELMARIVAAVTPTDAAVPVITLHDSLKHVDSSGRVIA
                     AFDREGLALAQTPQAATLVVLRAALEEAQAWGRKMTDEAAAFGAAGVVVHSVAGEPGN
                     QKLTEPGDEALVGGVLAARSMPLVAPVIGEGQRAGIGYDAHRFGEGGTLHLGGLEFPG
                     EPGLAGHSDGDVALHAVIDALLGAAAAGDIGSLYPPDDARWADADSAELVRGAVSTLG
                     DVGWLPVSIDLTIVAAHPAIEPRRDEMAARLGELCGVEANAVGVKGTTSDGLGFAGAE
                     GIAVFAVAVVAHA"
     gene            complement(1249..2307)
                     /locus_tag="VIN32_04520"
     CDS             complement(1249..2307)
                     /locus_tag="VIN32_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEY8921877.1"
                     /translation="MTTFIRLVGALLGTLLGFALGLLILTNADTVIDAPNRPAFLMAM
                     VAATLLFGYLAIPYITTRPARWAVDRLAHAGAGEFALGVAAIVVGLLVGVLAGLPLIA
                     IGGWAGSVLPPVVAVVLALLLLWATMYKRDVLLPAVGGILPGARRRGGFSQIVVDTSS
                     VIDGRIVDVGRTGFILGTLVVPRFVLDELQRIADSPDAIRRNRGRRGLEMLTALQKDS
                     ISPVEVTEETYPEIGEVDAKLVAFARDHGAAILTNDFNLNRVAELQGIRVLNINELAN
                     AVKAVVHPGEEMTVKIIQEGKEPGQGVGYLDDGTMIVVESGSRSMNEEVGVTVTRVLQ
                     TVAGRMIFAQPRDAPTGG"
BASE COUNT          386 a          898 c          792 g          319 t
ORIGIN      
        1 gccggcggcg agcgcctcga ccaccggcct gcggccgccg atccactcgc tcatgcgtgg
       61 gcgacgacgg ccaccgcgaa gacagcgatc ccctcggcgc ccgcgaatcc gaggccgtcc
      121 gaggtggtgc cctttacgcc gacggcattc gcctcgacgc cgcacagctc ccccagccgg
      181 gccgccatct cgtcgcgccg aggctcaatc gccgggtggg cggcgacgat cgtcagatcg
      241 atcgagaccg gaagccagcc cacatccccc agcgtcgaga ccgcaccgcg gacgagctcg
      301 gcggagtcgg catctgccca gcgggcatcg tcgggcgggt agaggctgcc gatgtcgccc
      361 gccgcagccg cacccagcag cgcgtcgatg accgcatgca gcgcgacgtc accgtcggag
      421 tgccctgcca gccccggctc gccaggaaat tccaacccgc cgaggtgcag cgtccctccc
      481 tcgccgaagc gatgcgcgtc gtagccgatc cctgctcgct gaccctcgcc gatgaccggc
      541 gccaccagcg gcatcgaacg agcggcaagt actcctccaa ccagcgcctc gtcgccgggc
      601 tcggtcagct tctggttgcc gggctcgccg gcaaccgagt gaaccacgac gcccgcggcg
      661 ccgaaggcgg ccgcttcgtc ggtcatcttg cgcccccatg cctgcgcctc ttcgagtgca
      721 gcgcggagca cgaccagcgt cgcggcctgt ggtgtctggg ccagggccag gccctcgcga
      781 tcgaatgctg cgatgacccg gcctgagctg tcgacgtgct tcagcgaatc gtgaagggtg
      841 atgaccggga cggccgcgtc ggtcggggtg acggctgcca cgatgcgcgc catcagctcg
      901 ctcgaggctg ccggtcgcgc cgcgtcgtga atgaggaccg cggcatcctc cgggaagccg
      961 gcgcgcgtca gcgccgctaa accggccagc acagaatcgg ctcgctcctc gcccccggcg
     1021 acgacctcgg cccggccgcg agcggcctcc ggaatcgcgt cacggaatct ctccagtgcg
     1081 tcggggggcg ccacgaccac cacccagctc attcccggca cggccagcag ggtgtcgagc
     1141 gcccagcgcc aggcgggccg gccggagaac tcgatccaga gcttgtcgct ccccatccgc
     1201 cggctgctgc cggcggcaac cagcaccgcc ccgatcagca cggccggctc acccgccggt
     1261 tggggcatcg cggggctggg cgaagatcat ccgacccgcc accgtctgca gcacccgggt
     1321 cacggtcacg ccgacctcct cgttcatcga tcggctcccg ctctcgacca cgatcatcgt
     1381 cccgtcgtcc aggtagccga ccccctgccc gggctccttc ccctcctgga tgatcttcac
     1441 cgtcatctcc tcccccgggt gcacgaccgc cttgacggcg ttggcgagct cgttgatgtt
     1501 caggacccgg atcccctgca gctcggcgac ccgattcagg ttgaagtcgt tcgtcaggat
     1561 cgccgctccg tggtcgcgag cgaaggcaac cagcttggcg tcgacctcgc cgatctccgg
     1621 gtaggtctcc tcggtgacct cgactggcga gatcgagtcc ttctggaggg cggtcagcat
     1681 ctcgaggcca cggcgaccgc gattgcgccg gatggcatcg ggcgaatccg cgatccgctg
     1741 gagctcgtca aggacgaagc gcggcaccac cagcgtgccc aggatgaacc cggtgcggcc
     1801 gacgtccacg atgcggccat cgatcaccga gctggtgtcg accacgatct gggagaagcc
     1861 gccccgccga cgagcgcccg gcaggatccc gccgaccgcg gggagcagca cgtcccgctt
     1921 gtacatcgtg gcccacagca ggaggagcgc caggaccacg gcgaccacgg gcggcaggac
     1981 cgacccggcc cagccaccga tcgcgatcag cggcagcccc gccagcaccc cgaccagcag
     2041 gcccacaacg atcgccgcca cgccgagcgc gaactcgcca gcgcccgcgt gcgccaggcg
     2101 gtcgaccgcc cagcgggccg gtcgggtggt gatgtacggg atcgccaggt agccgaacag
     2161 cagggtggcg gcgaccatcg ccatcaggaa ggccggccga ttcggcgcat cgatgacggt
     2221 gtccgcatta gtcaggatga gcagccccag cgcgaacccg agcagtgtcc cgagcagggc
     2281 gcccacgagc cggatgaagg ttgtcatgag ccgatgagtc cgtgaggaat cgaatgcgcg
     2341 gggagatgta atggatgaat ccgatgaagc caatcccacg cccgccagcg gcggg
//
LOCUS       DATCQR010000133         2749 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_85327, whole genome shotgun sequence.
ACCESSION   DATCQR010000133 DATCQR010000000
VERSION     DATCQR010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2749
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85327"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..502)
                     /locus_tag="VIN32_04525"
     CDS             complement(<1..502)
                     /locus_tag="VIN32_04525"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8921878.1"
                     /translation="MTDPPRPRLTLALLALSHAVIHAQSALMPLIYPIVIVEFALNPR
                     DIGLFIAITTAVGGTMQLFYGFLTRWVSRPALLGGGQLLFAAGLLVSGLTRSLGQLLA
                     AISLARIGSSPQHPIGNALLSDVYPPERRGFAISAHISGGNVGTVLVPFVGLALIGSV
                     GWQATLA"
     gene            complement(499..1758)
                     /locus_tag="VIN32_04530"
     CDS             complement(499..1758)
                     /locus_tag="VIN32_04530"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8921879.1"
                     /translation="MLEMGRGFSAVRHRNFRLYWIGQIVSLAGTWMQSVSQPWLVLLL
                     GGSYIQLGIVVALQFAPSLALAPLGGVLADRVDKRRLLIFTQSVAALQAAVLFVLTAT
                     GVVEIWHIMVLALALGFVNALDMPVRQSFAAELVPREDLMNAITLNSASFNLARVIGP
                     AVAGITLALFGPAFNFAINAVSYLGVLEALRRMDPAAMLRLPRLDPYPSIRSSLSEGI
                     GYALRTPTVLWPLVLLAGVGTFGMNFQTLLPLFAKYTLELDADGFGALFAAMGVGSLL
                     GSLTLAFMGSRRPLIWMILGGATSFVIFEALLGLSRDPLAAFPLIVFVGLSSMLMINT
                     INVTVQRSVPDELRGRVMALYVTVFAGSTPIGGLIAGAVAQAWGPPAGFLVGAGATVL
                     FIGLAAWHLVVRGKARVELPPPSAVRA"
     gene            1826..>2749
                     /locus_tag="VIN32_04535"
     CDS             1826..>2749
                     /locus_tag="VIN32_04535"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEY8921880.1"
                     /translation="MRRPIAHLTGAAAVLAVLVAVSLTLAPGALSPGELPPPAASRQP
                     AASTGTQPPAVSPSPHGADGGGTAQPVELSPVSQAESAALQAAVDRAREAFGLTSVAV
                     GVSADARLGWSGASGPPRDGSSAPLSGTTPFAIASVTKTFTAAIVLQLVDEGRVNLDA
                     AVNDYLPELTIARGVTVRQLLSHTSGIADLLAPMRERLNAEPARIWQPAEVLALLGPS
                     IFAPGTSWAYSNTNYLIAGMLVERVTGHPFADELERRITGPLKLLGTGPPARGKLPEL
                     LGASWSSAFWTSAMLDSNAANLVRWGDALYGG"
BASE COUNT          471 a          942 c          984 g          352 t
ORIGIN      
        1 gtgcaagcgt ggcctgccag cccacggacc cgatcagggc aaggcccacg aacggcacga
       61 gcacggtccc cacgtttccc ccgctgatgt gggcgctgat cgcgaacccg cggcgctcgg
      121 ggggatacac gtcggagagc agcgcgttgc cgatcgggtg ctgcgggctg gatccgatcc
      181 gcgcgagcga gatcgcggca agcagctgcc ccagcgagcg ggtcaggccg gacacgagca
      241 gcccagctgc gaagaggagc tgaccgccgc ccaggagcgc gggacgagac acccatcggg
      301 tcaggaagcc atagaagagc tgcatcgtcc cgccgacggc cgtggtgatg gcaatgaaca
      361 gcccgatgtc gcgcgggttc agggcgaact cgacgatcac gatcgggtag atcaggggca
      421 tgagcgcgga ctgggcgtgg atcacggcgt gcgacagggc gaggagcgca agcgtcaacc
      481 gtgggcgtgg aggatcggtc aagcccgcac cgcggatgga ggcggcagct cgacgcgtgc
      541 cttgccgcgg accaccaggt gccatgcggc caggccgatg aacaggaccg tggccccggc
      601 accgaccagg aagccggccg gcggtcccca ggcctgcgcc acggcgcctg cgatgaggcc
      661 accgatcggg gtcgagccgg cgaagacggt cacgtacagg gccatcaccc gtccgcgcag
      721 ctcgtcgggc acgctgcgct gcacggtcac gttgatggtg ttgatcatca gcatcgacga
      781 gagccccacg aacacgatca gcgggaacgc ggccagcggg tcgcgggaga ggccgagcag
      841 cgcctcgaag atgacgaacg aggtggctcc gcccaggatc atccagatca atggccggcg
      901 tgagcccatg aaggcgagcg tcagcgagcc gaggagcgac ccgacgccca tcgccgcgaa
      961 gagcgcgccg aagccatcgg cgtcgagctc cagggtgtac ttggcgaaga gaggcagcag
     1021 cgtctggaag ttcatgccga aggtgccgac acccgccagc agcaccagcg gccacaggac
     1081 cgtgggcgtg cgcagggcat agccgatccc ctcggacagg ctggagcgga tcgaggggta
     1141 ggggtcgaga cgtgggagtc gcagcatggc ggcgggatcc atccggcgca gcgcctccag
     1201 cacgcccagg tagctcaccg cgttgatcgc gaagttgaag gcggggccga acagcgccag
     1261 cgtgatcccc gccaccgcgg ggccgatgac gcgagccagg ttgaacgacg ccgagttgag
     1321 ggtgatcgcg ttcatcaggt cctcgcgcgg gaccagctcg gcggcgaagg actggcgcac
     1381 cggcatgtcg agcgcgttca cgaagccaag cgccagggcc agcaccatga tgtgccagat
     1441 ctcgaccacg ccggtcgcgg tcaggacgaa cagcaccgcc gcctggagcg ccgccaccga
     1501 ctgcgtgaag atcagcagcc ttcgcttgtc gacgcggtcg gccagcactc cgccgagcgg
     1561 cgccagcgcc agtgacggcg cgaactgcag cgccaccacg atgccaagct ggatgtagct
     1621 gccgccgagc agcagcacca gccagggctg gctgaccgac tgcatccagg tgccggccag
     1681 gctgacgatc tggccgatcc agtacagacg gaagttgcgg tggcggacgg cgctgaagcc
     1741 gcgccccatt tcgagcattg atgggaatgc taccggtccg cggcgggtcg acgcggcttc
     1801 cgcggatgcc ccatgctgag gcccgatgcg acgaccgatc gcccacctga ccggtgccgc
     1861 ggcggtgctt gccgtgctgg tggccgtgtc gttgacgctc gcccccggag ccctttctcc
     1921 cggcgagctg cctcctcccg cagcatcccg gcagccggct gcaagcacgg gcacgcagcc
     1981 accagccgtc tcgccctccc cccacggagc cgatggcggc ggcaccgctc agcctgtcga
     2041 actcagtccg gtctcccagg cggagtcggc cgcgcttcag gccgcggtcg atcgagcccg
     2101 ggaggccttc ggcctgacct cggtggcggt cggcgtctcg gccgatgcgc ggctcggctg
     2161 gagcggtgcc tccggaccgc cgcgcgacgg gtccagcgcg ccgctcagcg ggacgacgcc
     2221 gttcgcaata gccagcgtca ccaagacctt cacggcggcg atcgtgctgc agctcgtcga
     2281 tgagggccgc gtgaacctcg acgcagcggt caatgactac ctgccggagc tgacgatcgc
     2341 ccgcggcgtg acggtgcgcc agctgctcag ccacacctcc gggattgcag acctgctggc
     2401 accgatgcgc gagcggctca atgctgaacc ggcacgcatc tggcagccgg ccgaggtgct
     2461 ggccctcctc gggccgtcaa tcttcgcgcc gggcacgagc tgggcctatt ccaacacgaa
     2521 ctacctgatc gccgggatgc tggtcgagcg cgtcaccggc cacccgttcg ccgacgagct
     2581 ggagcgtcgc atcaccggcc cgctgaagct gctgggcacc ggtccgccgg cgcggggcaa
     2641 gctgccggag ctgctcggcg cgtcctggag cagcgccttc tggacatcgg ccatgctcga
     2701 ctcgaatgcg gccaacctgg tgcgctgggg tgatgcgctg tacggcggg
//
LOCUS       DATCQR010000134         1653 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_70181, whole genome shotgun sequence.
ACCESSION   DATCQR010000134 DATCQR010000000
VERSION     DATCQR010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1653
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70181"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..636)
                     /locus_tag="VIN32_04540"
     CDS             complement(<1..636)
                     /locus_tag="VIN32_04540"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY8921881.1"
                     /translation="MSGQVAIVTGASRGIGRGVAVGLGEAGWTVYVTARTVRDGESDR
                     PGSITRTAEQVTRVGGRGIPMRSDHSVDSETEAVFRRVEDEQGRLDLLVNNATSYSTD
                     LGPREDAMFWDLPVEEWDQMHAVGLRSHYIASACAARMMVPRRSGLIVNISSMGAIKY
                     SGNVSYYVVKAAVDMLTLSTAEELRPHKVAVVSFWPRLTRTEALIAHPGQFL"
     gene            complement(761..1603)
                     /locus_tag="VIN32_04545"
     CDS             complement(761..1603)
                     /locus_tag="VIN32_04545"
                     /inference="COORDINATES: protein motif:HMM:NF024954.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HEY8921882.1"
                     /translation="MLGWHRALVRRKWAAYGGRPRRGRPPISVECRQLIMRMACENPR
                     WGYFRIRGELLKLGHQVAATTIRSVLLAAGVPPSGRRSQLTWKQFLSAHAETLVAADF
                     FSVDTIFFKRLYVLIYVHLASRRVLLASCTSEPNAAWVTQQARNLSWKLEDEGIKLSV
                     LIHDRDKKFALQADRVLQSQGARVILTPLMAPKANAYAERWIGNCRRECLDWMLVVNQ
                     RHLETVIDEYCLHYNDERPHRSRDLRPPTSRGDGMRVAGGWIERSTRLGGLLSDYHQM
                     PVAA"
BASE COUNT          302 a          543 c          500 g          308 t
ORIGIN      
        1 caggaactga ccggggtgcg caatgagcgc ttcggtccgt gtcaacctcg gccagaacga
       61 gacgaccgcc accttgtgtg ggcgcagctc ctctgccgtg ctcagcgtga gcatgtccac
      121 cgctgccttg acgacgtagt aggacacgtt gcctgagtac ttgatggcgc ccatcgacga
      181 gatgttgacg ataagcccac tgcgccgcgg gaccatcatc cgggcggcgc acgcgctggc
      241 gatgtaatgc gacctcaggc cgaccgcgtg catctggtcc cactcctcga cgggcaggtc
      301 ccagaacatg gcgtcctcgc gcgggcccag gtctgtgctg taggacgtcg cattgttgac
      361 caggagatcg agcctaccct gctcatcttc gacgcgacga aatacggctt ctgtctccga
      421 atccacgctg tggtccgagc gcatcgggat gccacgccca ccgactcgcg ttacctgttc
      481 agcagtcctt gtgatcgaac cagggcgatc gctctcgccg tcgcgaactg tgcgggcagt
      541 gacgtagacc gtccatcccg cctcacccag cccaactgct acaccgcgtc cgattccgcg
      601 gctggccccg gtgacgatag cgacctggcc tgacatcgcg ggtcaacata ctggaagaag
      661 tgctccgccg tttttgccta gaacttgccg accccgcctc gcctccaagc atgagcagag
      721 ctcgacctcc tgcgccatag tgtcgggttc gttaatgacg tcacgcggct acaggcatct
      781 ggtgatagtc gctgagtaat ccgccgaggc gagtgctccg ctcgatccag ccgccggcca
      841 cccgcatacc atcgccgcgc gacgttggcg gtcgtaggtc gcggctgcga tgcgggcgct
      901 catcgttgta gtgcaggcaa tactcgtcga tgaccgtttc gaggtggcgc tggttgacga
      961 ctagcatcca gtcgaggcat tctcgacggc agttcccgat ccagcgctcg gcatacgcat
     1021 ttgccttagg cgccatcagc ggagtcaaga tcacgcgggc gccctgcgac tgcaagaccc
     1081 gatcagcctg gagcgcaaac ttcttgtccc gatcgtggat caagacgctg agtttgatgc
     1141 cttcgtcctc gagcttccag ctgagattgc gagcttgttg ggtgacccag gcggcattcg
     1201 gttccgaggt acagctcgcc agcaagactc gacgtgacgc cagatgcaca tagataagga
     1261 cgtagagccg cttgaagaag atcgtgtcca cgctgaagaa gtcggccgca accagcgtct
     1321 cggcatgcgc gctcaggaat tgcttccagg taagttgaga ccgtcggccg gaaggcggaa
     1381 ccccggcggc gagcagcacc gagcggatcg tggtcgcagc gacctgatgg ccaagcttca
     1441 acagctcgcc ccggattcgg aagtagcccc agcgcggatt ctcgcatgcc atccgcatga
     1501 tcagctggcg gcactctact gagatcggcg ggcgcccacg gcgcggtcgg cccccgtagg
     1561 cagcccactt ccgtcgcacc aatgcgcggt gccacccgag cactgtctcc ggtttgacca
     1621 gcaatgccgc ccagcccgat ctcgggatgc gtt
//
LOCUS       DATCQR010000135         2069 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_8018, whole genome shotgun sequence.
ACCESSION   DATCQR010000135 DATCQR010000000
VERSION     DATCQR010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8018"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="VIN32_04550"
     CDS             <1..371
                     /locus_tag="VIN32_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921883.1"
                     /translation="LARRLDEMAPTLASGLDAEREAAPVAAPAKRGPGRPRKTAAVAR
                     VRKSGPKVALHDEITAVIRELGPQTAAELASAIAARGRYQAPRSAKPLDAATVNARVS
                     NPTYRGRFKRREHRIGLATE"
     gene            complement(385..588)
                     /locus_tag="VIN32_04555"
     CDS             complement(385..588)
                     /locus_tag="VIN32_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018304878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HEY8921884.1"
                     /translation="MSVAKVTELSCTSNDSFEDAIRQGMARANKTLRGVRGAWVKELR
                     VNTGSEGAVEFQANILVTFILEG"
     gene            complement(622..1671)
                     /locus_tag="VIN32_04560"
     CDS             complement(622..1671)
                     /locus_tag="VIN32_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020515251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEY8921885.1"
                     /translation="MAASRDPSADLRRVAFLLEVANEPTYRVRAFRNAAATVDGMPAT
                     ELAERARRGTLRDLAGIGEVLARTIAESLRGEEPVYLRRLEATGGQPTAELGAALRAA
                     LRGDCHVHSDWSDGGSPIREMAEAARDLGHDYMVLTDHSPRLTVANGLSAERLEQQLD
                     DVARLNAELVPFRILTGIEVDILSDGSLDQDADLLARLDLVVGSVHSELRMDEKPMTR
                     RLVTALADPHLDVLGHMTGRMITGKRRRPPSDLDAEIVFAAAVQFDKAIEVNSRPERL
                     DPPKRILRLAVEAGCRFSIDSDAHAPGQLAWLPFGCDRAAACGVAEASVVNAMDADGL
                     LAWTATHEEAGGDRS"
     gene            complement(1671..>2069)
                     /locus_tag="VIN32_04565"
     CDS             complement(1671..>2069)
                     /locus_tag="VIN32_04565"
                     /inference="COORDINATES: protein motif:HMM:NF014212.4"
                     /note="phosphotriesterase homology protein; PhP; YhfV;
                     member of a family of proteins related to
                     phosphotriesterase (PTE); Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HEY8921886.1"
                     /translation="LDQLRIFEEEGVEPARVVIGHADSYPVLDHYLAILDRGANLQFD
                     FIGHRFGTEEAAEPRLVELIVELLERGYGTQLLLSQDVAHNSQLRANGGFGYAYLQQH
                     FLPTLRTAAVAEGEIAQMTIDNPRRILTIE"
BASE COUNT          323 a          738 c          694 g          314 t
ORIGIN      
        1 agctcgcccg gcgcctcgat gagatggctc caaccctcgc ctccgggctg gatgccgagc
       61 gcgaggcggc acccgtcgca gcgcctgcga agcgcggacc ggggcggcca aggaagactg
      121 ctgccgttgc ccgcgtcagg aagtccggcc cgaaggtcgc gctgcatgac gagatcaccg
      181 cggtgatcag ggagcttgga ccgcagacag cagcggagct ggcctcagcc atcgcagcac
      241 gcggccgcta ccaggccccg cgcagcgcca agccgctcga cgcggcgacg gtcaacgcgc
      301 gggtctcgaa cccgacctat cgcggccgct tcaagcgtcg cgagcaccgc atcgggctcg
      361 ccaccgagta gggctccggc cgcctcatcc ctcgaggatg aaggtgacca ggatgttggc
      421 ctggaactcc accgcgccct cgctgcccgt gttcacacgc agctccttga cccaggcgcc
      481 gcggactccg cgcagcgtct tgttcgcccg cgccatgccc tggcggatgg cgtcctcgaa
      541 cgagtcattg gacgtgcagc tcagctcggt gaccttcgcg actgacatgg cggatctcct
      601 cttgaagggc gggcgtcaag tctaggaccg atctcctccg gcttcctcgt gcgtggctgt
      661 ccacgccagc agcccgtccg cgtccatcgc gttgacgacc gaggcctctg ccacgccgca
      721 ggccgcggct cgatcgcagc cgaaggggag ccatgccagc tgcccgggtg catgagcgtc
      781 cgaatcgatc gagaagcggc aaccagcctc caccgccagg cgcaggatcc gcttcggtgg
      841 gtcgaggcgc tcgggacgcg agttgacctc gatcgccttg tcgaactgca ccgctgcggc
      901 gaagacgatc tcggcgtcga gatccgaggg tggccgtcgc cgcttgccgg tgatcatccg
      961 accggtcatg tggccgagca cgtcgagatg tggatcggcc agcgccgtga ccagccgcct
     1021 ggtcatcggc ttctcatcca ttcgcagctc ggaatgcacg ctgcccacca ccaggtcgag
     1081 ccgcgccagc aagtcggcat cctggtcgag cgagccgtcg ctcaggatgt cgacctcgat
     1141 cccggtcagg atccgaaatg gcaccagctc cgcgttgagg cgcgccacgt cgtccagctg
     1201 ctgctcgagc cgctcagcag agagcccatt ggcgacggtg aggcgcggcg agtggtcggt
     1261 cagcaccatg tagtcgtggc ccaggtcgcg cgccgcctcc gccatctcgc gaatcggcga
     1321 gccgccgtcc gaccagtccg aatgaacgtg gcagtcgccg cgcagggcgg cccgcaatgc
     1381 cgctcccaac tcggcggtgg gctgcccgcc ggtcgcctcc aggcgtcgga ggtagaccgg
     1441 ctcctcgccg cgcagcgact cggcgatcgt tcgcgccagc acctcaccga ttccggccaa
     1501 gtcgcggagc gtccctcggc gggcccgctc ggcgagctcc gtggccggca tgccatcgac
     1561 tgtagccgcg gcgttccgga aggcccgcac ccggtaggtc ggctcgttgg cgacctccag
     1621 caggaaggcg acgcgccgca ggtccgcaga agggtcgcgc gaggcagcca ctactcgatc
     1681 gtcaggatgc ggcgcgggtt gtcgatcgtc atctgagcga tctcgccctc ggcgaccgcg
     1741 gcagttcgga gcgtcggcag gaagtgctgc tgcagatagg cgtagccgaa ccctccgttg
     1801 gcgcgcagct ggctgttgtg tgccacgtcc tggctgagca gcagctgcgt cccataccca
     1861 cgctccagca gctcgacgat cagctcgacc aggcgcggct ctgcagcctc ctcggtgccg
     1921 aaccgatgcc cgatgaagtc gaactgcagg ttggcgccac gatcaaggat cgccaggtag
     1981 tggtccagga ccgggtacga gtcggcatgg ccgatgacga cccgcgctgg ctcgacgccc
     2041 tcctcctcga agatgcgcag ctggtcgag
//
LOCUS       DATCQR010000136         4524 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_23371, whole genome shotgun sequence.
ACCESSION   DATCQR010000136 DATCQR010000000
VERSION     DATCQR010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4524
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23371"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /gene="ffs"
                     /locus_tag="VIN32_04570"
     ncRNA           <1..227
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VIN32_04570"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            291..1892
                     /gene="dnaX"
                     /locus_tag="VIN32_04575"
     CDS             291..1892
                     /gene="dnaX"
                     /locus_tag="VIN32_04575"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEY8921887.1"
                     /translation="MVEPAAAASPEPSAIYRRWRSQRFEELIGQEPIVTTLRNAVAAD
                     RIAHAYLFVGPRGTGKTSMARILAKAINCTERGADGEPCDRCPACVSIREGRAMDVIE
                     IDAASHGLVEDARDLVMRALTAPAELRRRVYIIDEVHMLSAHAFNALLKLVEEPPPHV
                     VFILATTDTHKVPATVISRTQRFDFRRISVPLIVAKLTRIVEADGAQADPDALEVIAQ
                     LADGGMRDAESLLDQVLAYAGDRVTADSVREAVGLADEAEIGLLIDAYLTGDAGAALD
                     RIEALADVGRDMGQVAAQAEGEARRRLLRSASDPPLARRLASMLRTLAEAATVGAREG
                     RARLALELLAVEAPRPAAAVQAPVAVSIAAPAPPPAPVGAPQDQPTPPKARVDPTPPV
                     ETPPSAQPHAANGDAPPTELSGLRSRWGDVVERAAPAIKPLLRECRPIAVDGVRVTLA
                     FPEERSFMRAKAATRAPAIEQLLTTVLGGSWAIECVASNVELEPLTVAEAIVSSPGDS
                     EGLALLEGVLRITGGELVDAPEVRS"
     gene            1889..2206
                     /locus_tag="VIN32_04580"
     CDS             1889..2206
                     /locus_tag="VIN32_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005489260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEY8921888.1"
                     /translation="MNIGQIAKMAQQMQTQMAQAQAELREMTLEATAGGGAVRVVVTG
                     AQEVRLIEIDPAAVDPAEVEMLQDLVMAAVNDAIARSKELERERMASVAGGMGLPGLP
                     GLP"
     gene            2217..2831
                     /gene="recR"
                     /locus_tag="VIN32_04585"
     CDS             2217..2831
                     /gene="recR"
                     /locus_tag="VIN32_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HEY8921889.1"
                     /translation="MSALIAPVARLIEEFSKLPGVGRKTAQRLTYHMLRAPADEARGL
                     ASALVAVKEEVAYCSTCCNITDRGVDPCAFCADDRRDATRICVVEEPLDVLAIDRTGE
                     YRGRYHVLHGAISPIDGIGPDQIKARELVARVAGGGIEEVILATNPNLEGEATAMYLA
                     DLLAPYVPMVSRIARGLPVGGDLEYADDVTLIRSLQGRRQVDLS"
     gene            2874..4235
                     /gene="xylA"
                     /locus_tag="VIN32_04590"
     CDS             2874..4235
                     /gene="xylA"
                     /locus_tag="VIN32_04590"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011333729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HEY8921890.1"
                     /translation="MSQDFFPDVQGRIPFGGIGSDDPLSFKVYQPDRFVLGKRMEDHL
                     RIAVCCWHSFNWPGSDVFGMGTFDRPWLDPRLDPLIAARAKAEAAFEFFDKLGVPFFC
                     FHDRDIAPEGKTFAESMTNLLTMVDELEAHMQRTGVRLLWGTANLFSHPRYAAGAATN
                     PDPEVFAYAAAQVKTMLEATHRLGGSNYTLWGGREGYETLLNTDLVREERQFARFLEM
                     VADHKRRIGFKGALLLEPKPQEPTKHQYDYDAATVYGFLARHDLEGEFRVNLEANHAT
                     LAGHSFHHEVAQAVALGVFGSVDVNRGDYQNGWDTDQFPNSVDELSLALYEILRAGGL
                     TDGGFNFDAKLRRQSVDRTDLFHAHIGGMDTLARALLAAAAMVEHGEIERLREQRYAG
                     WAGDLGKSILTKDTLLELLAGRVATGEIDPSPRSGRQEQLENLVNRAIWEVDSAIDRG
                     DRD"
     gene            4240..>4524
                     /locus_tag="VIN32_04595"
     CDS             4240..>4524
                     /locus_tag="VIN32_04595"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HEY8921891.1"
                     /translation="MGLVLGIDVSTTATKAILIEPDGAVRGIGSADLRVSMPQPGWSE
                     QDPADWWSAAGSAISQALAAASASGADVEAVGLTGQMHGAVLLDAGGAVLR"
BASE COUNT          712 a         1557 c         1587 g          668 t
ORIGIN      
        1 tccgctacag cgggactgaa tccccggccg aggccgatcc gccttcgtcc cctacgaaca
       61 tcgaccgacg ttgagagtcg ggtcccacgc agcgagagtc cgtgaacccc gtcaggtccg
      121 gaaggaagca gcggtaagcg atcgccctcg ggtgccgtgg gacgacctgg ctggagccgg
      181 tcgatggacg tgtgggcgga agcgatcgat cgacgccggg tgcacggcca atttcgtctg
      241 ttccacggcg accgcgaccg aaccgcggtt cggtgtacgg ttaggagacc atggtcgaac
      301 ccgcagccgc tgcatctcct gagccatcgg ccatctatcg ccgatggcgc tcgcagcgct
      361 tcgaggagct gatcgggcag gagccgatcg tgaccaccct gcgcaatgcg gtggccgccg
      421 accggatcgc gcacgcctac ctcttcgtcg gcccccgcgg cacaggcaag acctccatgg
      481 cacgcatcct ggccaaggcc atcaactgca ccgagcgcgg agccgacggc gagccgtgtg
      541 accgttgccc cgcctgcgtc tcgatccgcg agggccgagc gatggacgtg atcgagatcg
      601 atgccgccag ccacggcctg gtcgaggatg cccgcgacct ggtgatgcgg gcgctgaccg
      661 caccggccga gctgcggcga cgcgtctaca tcatcgacga ggtgcacatg ctcagcgcac
      721 acgccttcaa tgcgctgctg aagctggtcg aggagccgcc gccacacgtc gtcttcatcc
      781 tcgccaccac ggacacgcac aaggtgcctg ccacggtcat cagccgcact cagcgcttcg
      841 acttccggcg catatcggtc ccgctgatcg tggccaagct gacacggatc gtcgaggcgg
      901 acggcgccca ggccgatccc gatgcgctcg aggtgatcgc ccagctcgcc gacggcggga
      961 tgcgggatgc tgagtcgctg ctcgaccagg tgctcgccta cgcgggcgat cgcgtcaccg
     1021 cggacagcgt gcgcgaagcc gtgggactgg ccgacgaggc cgagatcggc ctcctgatcg
     1081 acgcctacct gacgggcgat gcgggggccg ctctcgaccg gatcgaggcg ctggccgacg
     1141 tcgggcgcga catgggccag gtcgccgccc aggccgaggg agaggcgcgg cggcggctgc
     1201 tgcgatcggc gtctgatccg cccctggcgc gccgcctggc gagcatgctg cgcacccttg
     1261 ccgaggcggc gaccgtcggt gcccgcgaag gtcgcgcgcg actggcgctc gagctgctgg
     1321 cggtcgaggc gccacgcccg gctgccgccg tccaggctcc agtggcagta tcaattgcgg
     1381 ctccggcacc acccccagcg ccggtcgggg cgccgcagga ccagccaact ccgcccaagg
     1441 ctcgggtgga cccgaccccg cccgtggaga cgccgccatc ggcccagccg cacgctgcca
     1501 atggagacgc gccaccgacc gagctgagtg gccttcgatc gcggtggggc gatgtcgtcg
     1561 agcgtgcggc ccccgccatc aagccgctcc tgcgcgagtg ccggccgatc gcggtcgacg
     1621 gcgtacgagt gacgctggcg ttccccgagg agcgctcctt catgcgtgcc aaggccgcga
     1681 cacgggctcc ggcgatcgag cagctgctga ccaccgtgct gggagggagc tgggcgatcg
     1741 agtgcgtggc cagcaacgtg gagctcgagc cgctgacggt ggccgaggcg atcgtctcca
     1801 gccccggcga cagcgagggc ctcgcattgc tggagggggt cctgcgcatc accggcggcg
     1861 agctggtgga cgcccccgag gtcagatcat gaacatcggc cagatcgcca agatggcgca
     1921 gcagatgcag acccagatgg cgcaggcaca ggccgagctg cgagagatga cgctggaggc
     1981 caccgcgggc ggcggcgcgg tgcgtgtggt cgtcaccggc gcgcaggagg tgcgtctgat
     2041 cgagatcgat ccggctgcgg tcgacccggc cgaggtggag atgctgcagg acctggtcat
     2101 ggcggcggtc aacgatgcga tcgcgcggag caaggagctc gagcgcgagc ggatggcgtc
     2161 ggtggccggc gggatgggcc ttcccggctt gccggggctg ccttgaatcg gccggcgtga
     2221 gcgccctgat cgcgccggtc gcgcgcctga tcgaggagtt cagcaagctg ccgggggtcg
     2281 ggcgcaagac cgcccagcgc ctcacctacc acatgctccg tgctcccgcg gacgaggccc
     2341 gcggccttgc ctccgcgctc gtggccgtga aggaagaggt cgcctactgc tcgacctgct
     2401 gcaacatcac cgaccgcggc gtcgacccct gcgccttctg cgccgatgac cgacgcgacg
     2461 cgacgcgcat ctgcgtggtg gaggagccgc tggacgtcct ggccatcgat cgcaccggcg
     2521 agtaccgcgg ccgctatcac gttctccatg gcgccatcag ccccatcgac ggcatcggtc
     2581 cggaccagat taaggcgcgc gagctggtgg cacgcgtggc gggcggcggt atcgaggagg
     2641 tgatcctggc caccaatccc aacctggagg gggaggcgac cgccatgtac ctggccgacc
     2701 tgcttgcgcc gtatgtcccg atggtgtctc gcatcgctcg agggctgccg gtcggcggcg
     2761 acctggaata cgccgacgac gtgaccctca tccggtcgct ccagggtcgc cgccaggttg
     2821 acctgtcctg aggtggtcgt ctacgctcga cctccccgcc agagattctc gagatgagcc
     2881 aggacttctt ccccgatgtg cagggccgga tcccgttcgg tggcatcggc tcggacgatc
     2941 cgctctcctt caaggtctac cagccggacc gattcgtcct cggcaagcgc atggaggacc
     3001 acctccgcat cgcggtctgc tgctggcact ccttcaactg gccgggctcg gacgtctttg
     3061 gcatggggac cttcgaccgc ccctggctcg accctcgcct cgacccgctg atcgcagccc
     3121 gggccaaggc tgaggcggcg ttcgagttct tcgacaagct cggagtcccc ttcttctgtt
     3181 tccacgatcg cgacatcgct cccgagggca agaccttcgc cgaatcgatg acgaacctcc
     3241 tgacgatggt cgacgagttg gaggcccaca tgcagcggac cggcgtccgc ctgctgtggg
     3301 ggactgcgaa cctgttcagc cacccgcgct acgccgcggg agcggccacc aacccggatc
     3361 ccgaggtctt tgcctacgcg gccgcccagg tcaagacgat gctcgaggcc actcatcggc
     3421 tgggtggttc caactacacc ctgtggggcg gtcgtgaggg ctacgagacg ctgctgaaca
     3481 ccgacctggt gcgcgaggag cgtcagttcg cccgcttcct ggaaatggtg gcggaccaca
     3541 agcgccggat cggcttcaag ggagccctgc tcctcgagcc gaagccacag gagccgacga
     3601 agcaccagta cgactacgac gccgcaacgg tgtacggctt cctggcgcgg cacgacctcg
     3661 aaggcgagtt ccgcgtcaac ctggaggcaa atcacgccac tctcgccggc cacagcttcc
     3721 accacgaggt ggcgcaggcc gtcgcgctgg gcgtcttcgg cagcgtggac gtgaaccgcg
     3781 gggactatca gaacggctgg gacacggacc agttcccgaa ttcggtcgac gagctgtcgc
     3841 tggccctgta cgagatcctg cgtgccggcg ggctgaccga cggcggcttc aatttcgacg
     3901 cgaagctgcg gcgccagagt gtcgatcgga ccgatctgtt ccacgcgcac atcggcggga
     3961 tggacaccct cgcccgggcg ctcctcgcgg ccgccgccat ggtcgagcac ggtgagatcg
     4021 agcgactgcg cgagcagcgc tatgccggct gggccgggga cctgggcaag tcgatcctca
     4081 ccaaggacac gctgctcgag ctgctggcgg ggcgggtcgc gactggcgag atcgacccga
     4141 gcccacgctc cggccgccag gagcagctcg agaacctggt gaaccgtgcg atctgggagg
     4201 tggattcggc aatcgaccgc ggcgacaggg actgagccga tgggcctcgt cctggggatc
     4261 gacgtctcga cgacggcgac gaaggccatc ctgatcgaac cggacggggc agtgcgcggg
     4321 atcggcagcg ccgaccttcg cgtctcgatg ccgcagcccg ggtggagcga gcaggatccc
     4381 gccgactggt ggtcagcagc cggatcggcg atcagccagg cgctggcggc cgcgagcgcg
     4441 tcaggcgccg atgtcgaggc ggtgggccta accgggcaga tgcacggcgc ggtcctcctg
     4501 gacgccggcg gggcggttct ccgc
//
LOCUS       DATCQR010000137        17587 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_147465, whole genome shotgun sequence.
ACCESSION   DATCQR010000137 DATCQR010000000
VERSION     DATCQR010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 17587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17587
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147465"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..423)
                     /locus_tag="VIN32_04600"
     CDS             complement(<1..423)
                     /locus_tag="VIN32_04600"
                     /inference="COORDINATES: protein motif:HMM:NF025033.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY8921892.1"
                     /translation="MSDMRQPRAFYEADSLSTEIFDALAASIIPGSSVEGDIDFYRRL
                     AGKTGGPILDVGCGTGRVAMALAADGHEVVGIDLSAPMLRLAEQRREALPAAVAARLS
                     FHQADMATLDLGRAFALVVTPSRVFQFMLTTDAQRQALE"
     gene            complement(420..851)
                     /locus_tag="VIN32_04605"
     CDS             complement(420..851)
                     /locus_tag="VIN32_04605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921893.1"
                     /translation="MTDVLGPELEKLLAGLDLDRWNSAVSEYLDRTDHPNELFNLLSG
                     GWLRGVVVPARRTNLLQHTVGLVEEWDAAHYPKRRHKGTPYYFLGMGYILLGDIDSEL
                     GDQGPRSTLATWAPPSHPSQPGGGRRRNALMQSPFEPTLRV"
     gene            complement(1103..1315)
                     /locus_tag="VIN32_04610"
     CDS             complement(1103..1315)
                     /locus_tag="VIN32_04610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921894.1"
                     /translation="MIWLRLHCSTFWVDVRLREINGRWIASADTPGGPSLGLGKGAIE
                     AVEHALEPFGAIVDELLASLPKAVLS"
     gene            1384..2658
                     /locus_tag="VIN32_04615"
     CDS             1384..2658
                     /locus_tag="VIN32_04615"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012784200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEY8921895.1"
                     /translation="MATIVQKYGGTSIGDPVRIRRVAERIVASAATGDRICVVVSAMG
                     DATDDLMGLAAEVSADPHERELDMLLTAGERISMALVSMAIIDLGRDAVSFTGAQAGI
                     ITDATHGRARIVEVQASRVAQALEGGTIAIVAGFQGVSTTDDVTTLGRGGSDMTAVAL
                     AAALGADVCEIYTDVDGVFTADPRVVPGARKLAAVSYEEMLELATSGARVLMSRCVET
                     ARNHGTPIHVRSSLTGNDGTWVTAEGERMEQAIIAGIAHETSEAKVTILGVPDRPGIA
                     ARVFRPLADAGVNIDMIVQNVSAAGHTDISFTLPKEDLRRAGPALEQVRIDVGASEVV
                     TDSDIARISLVGAGMKTHPGVAADMFDALADAGINIEIISTSPIRISCVVRAAEIERA
                     VQVVHDRFRLADPAIYREEPTTLAPSPSEAGS"
     gene            2655..3698
                     /locus_tag="VIN32_04620"
     CDS             2655..3698
                     /locus_tag="VIN32_04620"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020463983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEY8921896.1"
                     /translation="MKVAVVGATGVVGGEIMRILEERDLGITELVPIATARSTGRLLT
                     HRGRSLTVVAISPESFDGVDVALFDTPDDASLEWAPVAAGRGAVVIDNSASWRMDPEV
                     PLVVPEINPEAAQDRPKGIIASPNCTMLTLVLPLAALHRWAGVRRVILSSYQAASGAG
                     QAGADELVKQIAVAAQQPEIVGIGLGREAISRGSAFAHPLAMNVIPQIGSVRADGYTG
                     EEVKVGEETRKVLGLPDLRVTATCVRVPTVVGHAVSVHAEFDGAISADEARAILREAP
                     GVEVLDETAAGIYPTPLEAAGRDPCFVGRIRQDPADDHALEWFSVTDNLRKGAALNAI
                     QIAELFVPADRLG"
     gene            3717..3802
                     /locus_tag="VIN32_04625"
     tRNA            3717..3802
                     /locus_tag="VIN32_04625"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3750..3752,aa:Leu,seq:taa)
     gene            complement(3810..5024)
                     /locus_tag="VIN32_04630"
     CDS             complement(3810..5024)
                     /locus_tag="VIN32_04630"
                     /inference="COORDINATES: protein motif:HMM:NF025001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY8921897.1"
                     /translation="MPANPLLICAPSPELRARLEQLREAGYATTSVETAADSVNALSA
                     APFDLIIAEGLAVSGAIGRIRGATTRQVPLIVVSPAGDVEARIAFLEAGADDVISASF
                     ALEELQARVEALLIRTGKARPEGAAGRGAGRIVTFFAPKGGVGTTTLAVNSAVLLAGG
                     PDAMQGQAARVLLIDLDLQFGQVATHLNLSPRYDIADLCSDDQALTDPALAATYLTMH
                     TSGVSVLAGPSNPEADFRVTVDQLEKALRVLRPSFDHVLVDCGSRLDPRTLWMLEQAD
                     THVFVIFPEIAALRATTLMLGFLAETATLRATTHLVVNHIFPKELLKTRDVENLLRAK
                     PVAEIPYSEVDMIRAVNEGVPVVFSRPSSPASRAIQLLTVAIVGAEWRQRPSPTKKGP
                     AARRGGLRLGRR"
     gene            5228..5653
                     /gene="rpsF"
                     /locus_tag="VIN32_04635"
     CDS             5228..5653
                     /gene="rpsF"
                     /locus_tag="VIN32_04635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEY8921898.1"
                     /translation="MRRYELMLLFRPDLEDDKLQAAVEKVTRAIVNAGGALTKVSPWG
                     KRRLAYDIKHFREASYFLIHFDIAPAEVRGIERGILITEEILRHLVTVLEEKALGRAD
                     DELLEPAEAIPAKLDEAGDLLTDEPPDEVADEAEVAAKA"
     gene            5681..6154
                     /gene="ssb"
                     /locus_tag="VIN32_04640"
     CDS             5681..6154
                     /gene="ssb"
                     /locus_tag="VIN32_04640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEY8921899.1"
                     /translation="MTLNKMMIIGNLGADPELRYTPGGKAVAELRVAVNDRSKGPDGE
                     WQEETQWFRVELWEQAAERAAERLRKGHKVFAEGQLRAREWEGKDGQKRTSLEIRFAR
                     VQSLERREPGEGGGFSGGGFNEGASDAPPAVRPRGAGAQPAAAEVEAIDVDDIPF"
     gene            6187..6456
                     /gene="rpsR"
                     /locus_tag="VIN32_04645"
     CDS             6187..6456
                     /gene="rpsR"
                     /locus_tag="VIN32_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006877265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEY8921900.1"
                     /translation="MASPRSRRETADYYRDRRRRKVCNFCVDKAEAIDYKEVNRLRRY
                     LSERAKIEPRRKTGTCARHQRMLTVALKRARHVALLPVAPQHLRP"
     gene            6487..7605
                     /locus_tag="VIN32_04650"
     CDS             6487..7605
                     /locus_tag="VIN32_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:calcium antiporter"
                     /protein_id="HEY8921901.1"
                     /translation="MDVVLLVVALAVILLGAELFTNGIEWFGHRLNLAEGAVGSVLAA
                     VATAMPETLIPVIAIVGPAIFGGGSPNSSEIGVGAILGAPFMLSTLAMFVTGLAIVIY
                     TRRGRRETDMSVNVRVLGRDVIFFLVAYTIAIASAFLPADLRVGRWAVAIGLVVLYAV
                     YVRAHFTDPAEEGDGSDLNKLHLSRLAERMPGRMVDFDALPGEDDGHEHDGPVPRIRI
                     IVTQVVAALALIVLGAQVFVGAVEHLSSAIGLDPRILALVIAPIATELPEKFNSVIWV
                     RSGKDTLAMGNISGAMVFQSCLPTVLGILFTEWTFKASSALAFASAGAAFVSTVLIFG
                     VMLRRGRLSAWSLLLGGPIYLLYVAAALLLPAGAAAAH"
     gene            7713..8672
                     /locus_tag="VIN32_04655"
     CDS             7713..8672
                     /locus_tag="VIN32_04655"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HEY8921902.1"
                     /translation="MLKQETATATAEADRTAGLWAYFHGDFVPLAEAKISVMTHAFNY
                     GTAVFEGVRCYWNEEQEQLYGLDLLAHFERIHRSVRLLQLELRHTPEELVGITVELLR
                     RDGFREDAYIRPICYKSSEVIGVRLHNLESDLTIFAIPFGKYIDTEAGIRAQVSTWRR
                     TDDNAIPARSKITGSYVNGAFAKSEAQMNGYDEALMLTQEGHVSEGSAENLFIVRDGT
                     LITPPGSDNILEGITREHLMTMAGDLGIPVKERSIDRTELYMADEVFLCGTGAQLAPV
                     VEVDRRPIGTGKVGPITHRLHQIYFDAVRGRSDAYRDWLTPVY"
     gene            8729..10231
                     /locus_tag="VIN32_04660"
     CDS             8729..10231
                     /locus_tag="VIN32_04660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921903.1"
                     /translation="MSEARTQERYLWLQRLLEMVPGTVSWAVIIGPIWLSFSYPWLVA
                     YFVLSFDFYWLCRAIWFAGSVIVAHRRIRDVLATDWNARLESLDDPDIPEWRSFTHLA
                     LIPTYTESLETLRHTVRALAEADWATDRKICAIITRETDATGIANVEALRGEFGAAFA
                     DFIHILDPLEPGIVVGKSSAMAFGGRWLYRLLVRERGMDPSLLLVTDLDADYRVHPEY
                     FNYLTWMHVSDPNRDTQLYQPVPYFHNNVWQAPLLTRLFAAVLTQLQMWRSVLPEKLQ
                     SFGSYSTTLHLVHEVGYWATDAIPEDSRFYWKSYFTYGDRFRAVPLFIPIYGDAVRAK
                     TYWRSLATQYLQARRWAWGVTDLPYVVQNAIAHPEIPLSSRAWRVLNLFAEHINWAVA
                     PFVVAFGATVPLIVNPAFARTTLGQNLPIYASSMLSVALLALGELIWIEHRIVPPFPA
                     AWGRLRRMLVYVGWLGLPFVGIFFTNLPALDAQTRLLTGRYLEYRVTEKV"
     gene            10235..11362
                     /locus_tag="VIN32_04665"
     CDS             10235..11362
                     /locus_tag="VIN32_04665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921904.1"
                     /translation="MSELTTQITSVRLARDRARLFAAPVLFLLVAAAAVAGGLLLGGP
                     GGISLVAVGVLAIVVAAYLTALVSSYRLLVEPGVLRLQWLGGERPYRLVQGKVTRVGI
                     SGENAAALRPRFGALGWAVGPALLRGEPLELIRLSKRPPLIVAPTDRGRLAIAAAVES
                     ELLEAMTHAVHLQERLDEVASRRAAPSPVEVAPRPSAPAAPRVLTGIERTLIEQRLAA
                     ERAAALAAAEAERAGAAAAFVPEAPLVPEAVPAPGPREAPPAAEAPAAERRVARLRRR
                     GQWQRPSWVAVPGPVSVVSALPIALPLIGAGIAWIAVTVTGRPALPVDEARILSTALV
                     LVGPLGALGGLIARTWYPRIGGLVMVSSVAALAMLTRTILA"
     gene            11689..14874
                     /gene="ileS"
                     /locus_tag="VIN32_04670"
     CDS             11689..14874
                     /gene="ileS"
                     /locus_tag="VIN32_04670"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEY8921905.1"
                     /translation="MFKPVSGKLDVPGVEREQIEVWRERRTMERYLARNAQSERRWSF
                     LDGPITANGPMGVHHAWGRTYKDLYQRYHTMLGERQRYQNGFDCQGLWIEIDIERQLG
                     FNNKRQIEEFGIDRFVELCKARVDTMAALQTEQSKRLGMWMDWEHSYFTNSDENNYTI
                     WAFLKECHSRGLIYKGHDVMPWCPRCGTGLSNMEIATEGYREIRHLSLTVRLPITSPG
                     HEGEDLLVWTTTPWTLSSNVAAAVHPDLTYVLVEDADGRRSWISHGSQPRVMSDGTVV
                     RQVTGRELVGLAYSGPFDELPVQSGVEHRVIEWTEVSDEEGTGIVHIAPGCGQEDFHL
                     AKRDGLAVLDPIDEFGIFRDGYGWQTGRFAGATEDQATDVATDVAADLERKGLLVSRE
                     RYLHHYPVCWRCGTQLVFRLVDEWFIAMDPLRQPIGAATRQITWLPSGIGLEERELDW
                     LRNMSDWMISKKRYYGLALPIWECVDCAAWEVIGSRDELKQRAVAGWEAFDGHSPHKP
                     FIDQVEIDCRSCGGRSRRIGDVGNPWLDAGIVSFSTLRWNTDRDYWAEWFPADFVVEG
                     FPGQFRNWFYALLTMSTVLADGPMTRTIFGYATVLDEHGEEMHKSKGNAIPFDEAAEQ
                     IGADVMRWMYSAANPAVNLNFGYGPGHEVVRRFFLPLWNTYGFLVTYARLDGWTPGES
                     DDGMESARTLLDRWILSRLDAVVGDVRRALDAYDAARAARGIEGFVEDLSNWYVRRNR
                     RRFWKGELDDDKRAAYATLHEVLATVSRLLAPFVPHLADAIWGNLVAAVDPTASDSVH
                     LTDYPDRILGRAQPEIDAAVSLAREVVSLGRTARAASGLKTRQPLATVRVKLPASANG
                     QLSPDPTTAVELEAEILEELNAKSLEVLTDASELVERTLYPLLRVIGPRHGADVARIM
                     AGARSGDWKLLNDGGAEAGGVSLASDEFELTARARPGHEVAEAGDVLVALDTQVTPEL
                     LAEGLAREVAHRLQGLRKTAGLEVSDRVAASIDGEPALIAQLVPHRDWLAEEILAVTL
                     DLGGETDLGVSAAFEEVGMPDGRLRLGVRRA"
     gene            14902..15411
                     /gene="lspA"
                     /locus_tag="VIN32_04675"
     CDS             14902..15411
                     /gene="lspA"
                     /locus_tag="VIN32_04675"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEY8921906.1"
                     /translation="MSSEEPTNGGFTRAVILLLTALAILVADQVTKALVVANLALGEK
                     ARLLGDLVQVWHAQNRGAAFSLFQGGTVLFLIVSVLSIGLVAYFHRSLRDRTPWLHVV
                     LGIVLGGTLGNFLDRLRQGYVTDWLSVGIGDTRWPTFNVADSSVVVGIGILVVYLFVT
                     SPDRRQATA"
     gene            15408..16391
                     /locus_tag="VIN32_04680"
     CDS             15408..16391
                     /locus_tag="VIN32_04680"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEY8921907.1"
                     /translation="MSDVATAGGRVVTGAAVTGRFDLAVSAVAEISRAHAQRLISDGR
                     ALVDGRRARASDRLRGGEQIRVELSAPPDPTLRGESIPLRIAYEDGSMLIVDKDAGLV
                     VHPSHGHSTGTLVHALVGRAEERGEELGSIAGVGRPGIVHRLDKQTSGLIVVAKSDAA
                     QASLMQQFGARTIEKEYLALVRGTPPAPRGRIEAPVGRDPRDRQRMAVVAGGREAVTE
                     YETLGSADGHSLLALHPLTGRTHQLRAHLAYLGLPIAGDTRYGGGMGPGGLQRQFLHA
                     ARLTLDRPSDGRRLTAWSELPPDLSASLAASEIVTDRLPVGIGAALVEDQG"
     gene            16388..17026
                     /gene="gmk"
                     /locus_tag="VIN32_04685"
     CDS             16388..17026
                     /gene="gmk"
                     /locus_tag="VIN32_04685"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008731277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEY8921908.1"
                     /translation="MTGNGPADKPAKRLAFPNPLLVVVSGPSGVGKSTIVAELSRRHP
                     QVVPIVTATTREQREGELDKVHYHFLSPDEFQQLRDRKGLLEQAVVHDHWYGTPVDQV
                     RGILAAGRDAILTIDPQGARSVRTLVPDALLIFIMPPSIEDLDQRLARRGSEDGDERA
                     KRRRNAEAEMAAAGDYDYAIVNQTDHPDDAVEQIWEIIQAEARRDPPRRVQV"
     gene            17041..>17587
                     /locus_tag="VIN32_04690"
     CDS             17041..>17587
                     /locus_tag="VIN32_04690"
                     /inference="COORDINATES: protein motif:HMM:NF036997.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921909.1"
                     /translation="MAAGETVRVAVEALADRPERAFSYRLPPELGTAAAGSLLLVPYG
                     RRLALGYLLPGTEEPEPGIELRSVEAVVSAPMLTPDLLALAEEIAVYYCAPLGGTIGA
                     MLPPGLESRLERRWTTPEVSLLPDPLHAMLSGSDEISDAMLKRSLGPRWSPVADRLRR
                     SGALVARWSLRPPELKARRVRT"
BASE COUNT         2719 a         5987 c         6151 g         2730 t
ORIGIN      
        1 ctccagagcc tgccgttggg cgtcggtcgt gagcatgaac tggaagaccc gagaaggcgt
       61 cacaacgagc gcgaacgcgc gcccaaggtc gagcgtcgcc atgtccgctt gatggaacga
      121 gaggcgggcc gctacagcgg cgggcaacgc ctctcgccgt tgttctgcaa gacgcagcat
      181 cggcgcagag aggtcgatgc ccacgacctc gtgaccgtcc gccgcgaggg ccatggccac
      241 tcgaccggtg ccgcacccta cgtcaaggat cggtccgcct gtcttcccgg ccagtcggcg
      301 gtagaagtcg atgtcgccct cgacggagct gcccgggatg atgctcgccg ccagggcgtc
      361 gaagatctcg gtgctcaagc tgtcggcctc gtaaaaggcg cgcggctggc gcatgtcgct
      421 catacgcgca acgtaggttc gaacggggac tgcatcaagg cgttccgcct ccgcccgccg
      481 ccgggttggc tcggatgact cggcggcgcc catgtcgcca gggtcgaacg gggaccctga
      541 tcgccaagct cgctgtcaat atcgccgagc aggatgtagc ccatgccgag aaagtagtac
      601 ggcgttccct tgtgccgccg cttggggtag tgagcggcat cccactcctc aactaggcca
      661 acagtgtgtt gcagaaggtt tgtacgtcgg gccggcacca cgacacccct gagccagccg
      721 ccgctcaaaa ggttgaacag ctcgtttggg tggtctgtgc ggtcgaggta ttcgctaacg
      781 gcgctattcc accggtcgag gtcgagtccc gccagaagct tctcaagctc tggcccaagt
      841 acatcggtca ttggccagag tctaaggcgg agtggcttcg cagacagaca gtgcgcgatc
      901 tgcggccgca gatcggttcc atgcttctcg gtagcatcgc gaagtagcaa caggggcgaa
      961 cagccacggc gatcggcgcc tcaggatcgg ctactctgag cacgctggag tctccggcgg
     1021 acgcccgcag acgctatatc ccggactgca agaccgttta cacgggttcg agtcccgtcg
     1081 tcgtctccat cccccgagat cgttagctga gcaccgcttt cggcaggctg gccagcagct
     1141 cgtccacgat cgccccgaac ggctccaggg catgctcgac cgcttcgatc gctcccttcc
     1201 ccaatccgag gctcggcccg ccgggggtgt cggctgaggc gatccaccga ccgttgattt
     1261 cgcgcaggcg cacgtcgacc cagaaggtcg agcaatggag cctcagccag atcatggcgt
     1321 gaggtatagc gatctgacct tgcaggccac cttgcgattt ggccaacgtc gtaccctccg
     1381 cacatggcga ccatcgtcca gaagtacggc ggcacgtcga tcggcgatcc ggtgcggatc
     1441 cggcgggttg ctgagcgcat cgtggcgtcc gcggcaaccg gcgatcgcat ctgcgtggtc
     1501 gtctctgcca tgggagacgc gaccgacgac ctgatgggcc tagccgccga ggtgtcagcg
     1561 gatccgcacg agcgggagct ggacatgctc ctcaccgccg gcgagcgcat ctcgatggca
     1621 ctcgtctcga tggccatcat cgacctgggc cgcgacgcgg tttcgttcac cggggcgcag
     1681 gccggcatca tcaccgatgc gacccacggc cgggcacgaa tcgtggaggt gcaggcgagc
     1741 cgcgtggctc aggcgctgga aggcggcacc atcgccatcg tcgcaggctt ccagggggtg
     1801 tcgaccaccg acgacgtgac cacgctgggg cgtggcggct ccgacatgac tgcagtcgcc
     1861 ctggctgccg ccctgggcgc ggacgtctgc gagatctaca ccgatgtcga cggcgtcttc
     1921 acggccgacc cccgggtggt ccccggtgct cgcaagctcg ccgcggtctc ctacgaggag
     1981 atgctcgagc tcgccaccag cggggcgcgc gtgctgatgt cacgatgcgt cgagaccgcg
     2041 cggaatcacg ggactccgat ccacgtccga tcctccctga ccgggaatga cggaacgtgg
     2101 gtcacagcgg agggtgaacg gatggagcag gcgatcatcg cggggatcgc gcatgagacc
     2161 tcggaggcca aggtcacgat cctcggcgtc ccggatcggc cggggatcgc ggctcgcgtg
     2221 ttccgcccgc tcgcggatgc cggcgtcaac atcgacatga tcgttcagaa cgtgtcagcc
     2281 gccggccaca ccgacatctc cttcacgctc cccaaggagg acctgcggcg cgctgggccc
     2341 gcgctcgagc aggtgcgcat cgacgtcggg gcgtcggagg tcgtgaccga cagcgacatc
     2401 gcccggatct ccctggtcgg cgcgggcatg aagacccatc ccggcgtcgc cgccgacatg
     2461 ttcgatgccc ttgccgacgc cggcatcaac atcgagatca tctccacctc gccgatccgc
     2521 atctcgtgcg tcgtgcgcgc cgccgagatc gagcgtgccg tgcaggtcgt ccacgaccga
     2581 tttcgtctgg ccgaccccgc gatctatcgt gaggagccca ccacgctcgc gccatcaccc
     2641 tccgaggccg gctcatgaag gtggcggtgg tgggtgccac cggggtcgtg ggcggcgaga
     2701 tcatgcgcat cctcgaggag cgcgacctgg gcatcaccga gctggtgccg atcgccacgg
     2761 cgcgttcgac cgggcggctg ctcacgcatc ggggtcgctc ccttaccgtg gttgcgatca
     2821 gcccggaatc cttcgatggc gttgacgtgg cgctgttcga cacgcctgac gacgcatcac
     2881 tcgaatgggc gcccgtcgca gcgggccgcg gggccgtcgt catcgacaac tcggcttctt
     2941 ggcggatgga tccggaggtg ccgctggtcg tgcccgagat caaccccgag gctgcgcagg
     3001 atcggccgaa ggggatcatc gccagcccaa actgcacgat gctcacgctg gtcctgccgc
     3061 tcgccgcgct ccatcgctgg gccggggtcc ggcgcgtgat cctgtcgtcg taccaggccg
     3121 catcgggagc gggtcaggcc ggcgcagatg agcttgtcaa gcagatcgcg gtcgcggcgc
     3181 agcagccgga gatcgtcggc atcggcctcg ggcgcgaggc gatctctcgc gggagcgcct
     3241 tcgcgcatcc cctggccatg aacgtcatcc cgcagatcgg gtcggtgcga gcggacggct
     3301 acaccggtga ggaggtcaag gtgggggagg agacgcgcaa ggtgctcggc ctgcccgacc
     3361 tccgcgtcac cgccacctgc gttcgggtgc cgaccgtggt cggccacgcc gtgtccgtcc
     3421 acgctgagtt cgacggcgcc atctccgccg atgaggcccg cgcgatcctg cgcgaggcac
     3481 ccggcgtgga ggtcctggat gagacggcag ccggcatcta cccgacgccg ctggaggcgg
     3541 cgggtcgtga cccgtgcttc gtcgggcgca ttcgccagga cccggccgac gaccacgcgc
     3601 tggagtggtt cagcgtgacc gacaacctgc gcaagggcgc tgcgctcaac gcgatccaga
     3661 tcgccgagct gttcgtgcct gcggatcggc taggctgagc ccctacctcg tccgaagccg
     3721 gagtggcgga atggcagacg cggccggctt aaacccggcc gcccgcaagg gcgtgggggt
     3781 tcgaatccct cctccggcac cagcctgcgt cagcgtcggc cgaggcgcag ccccccgcgg
     3841 cgtgccgctg gacccttctt ggtcgggctc ggacgctgac gccattcggc cccgacgata
     3901 gccaccgtca ggagctggat cgcgcgcgag gcgggagagc tcgggcggct gaagaccacc
     3961 ggcacgccct cgttcaccgc acggatcatg tccacctcgg agtaagggat ctcggccacc
     4021 ggcttggcgc gcagcaggtt ctccacgtca cgcgtcttca gcagctcctt ggggaagatg
     4081 tggttgacca ccagatgtgt ggtggcacgc agcgtcgccg tctccgccag gaagccgagc
     4141 atcagggtcg tggcccgcag cgcggcgatc tcggggaaga tgacgaagac gtgggtgtcg
     4201 gcctgctcga gcatccacag cgtgcgcggg tcgaggcggg acccacagtc caccagcacg
     4261 tggtcgaacg acgggcgcag gacgcggagc gccttctcga gctggtcgac ggtaacccgg
     4321 aagtcggcct cggggttcga cgggccggcc aggacgctca cgcccgaggt gtgcatggtc
     4381 aggtaggtcg cggcgagtgc gggatcggtc agtgcctggt catcgctgca caggtcggca
     4441 atgtcgtagc gcggtgagag attcaggtgg gtggcgacct ggccgaactg gaggtcaagg
     4501 tcgatgagca ggacccgagc ggcctgtccc tgcattgcat cgggaccgcc cgccagcagg
     4561 acggcggagt tgaccgccag cgtggtcgtg ccgacgccgc ccttgggggc gaagaaggtc
     4621 acgatccggc ccgccccgcg accggccgct ccctcgggtc gtgccttgcc ggtgcggatc
     4681 agcagcgcct cgacgcgagc ctggagctcc tccagcgcga agctcgccga gatcacgtca
     4741 tccgctccgg cctcgaggaa ggcgatgcgg gcctccacgt ctccggccgg agagacgacg
     4801 atcaggggaa cctgcctcgt ggtcgctccg cggattcggc cgatcgcgcc cgagacggca
     4861 aggccctcgg cgatgatcag gtcaaatggc gccgcgctca gcgcgttgac ggaatccgcc
     4921 gcggtctcca cgctcgtcgt cgcatagcca gcctctcgca gctgttcgag gcgggcgcgc
     4981 agctccgggc tgggggcgca gatcaggagc gggttggccg gcacggggtt cctcgtcgag
     5041 ggcggagcaa atcggtcgca cagtataggg tgccctccac gcgcgttgcg acggggggcc
     5101 ttgaaccgta tactgccgat ggcctccagc gatggaggtc gttccgcaag atgggctcct
     5161 ttctgccgca ctcctcatgg cgcaagccga gcgtgtcgca agcccagaga gaggaggaga
     5221 tcggccgatg cgccgttacg aactgatgct cctgtttcgg ccggacctgg aggacgacaa
     5281 gctccaggcg gcggtcgaga aggtgacccg tgccatcgtc aacgctggcg gggcgctcac
     5341 caaggtctcg ccgtggggca agcgccgcct tgcctacgac atcaagcact tccgcgaggc
     5401 gagctacttc ctgatccatt tcgacatcgc ccccgccgag gtgcgcggca tcgagcgcgg
     5461 gatcctgatc accgaggaga tcctgcgcca cctggtcacc gtgctcgaag agaaggcact
     5521 ggggcgggcc gatgacgagc tcctcgagcc ggctgaggcg attcccgcga agctcgacga
     5581 ggcaggcgac ctcctgaccg atgagccccc agacgaggtc gccgacgagg ctgaggtggc
     5641 cgccaaggcg tagcgccggc ggtcggagga gaggaacacc atgaccctga acaagatgat
     5701 gatcatcggc aacctgggcg ccgaccctga gctccgctac acccccggcg ggaaggcggt
     5761 cgccgagctg cgcgtcgccg tgaacgaccg gtcgaagggc ccggacggcg aatggcagga
     5821 agagacccag tggttccgcg tcgagctgtg ggagcaggcc gccgagcgcg ccgccgagcg
     5881 gctgcgcaag gggcacaagg tctttgccga gggccagctg cgcgcccgtg agtgggaggg
     5941 caaggatggc cagaagcgaa cctcgctcga gatccgcttc gcccgcgtgc agtcgctcga
     6001 gcgacgcgag cccggcgagg gcggcggctt cagcggcggt ggcttcaacg agggcgcttc
     6061 cgatgctcca cccgcggtca ggccgcgcgg cgcgggggcc cagcctgccg ctgccgaggt
     6121 cgaggccatc gacgtcgacg acatcccgtt ctagctgaca cgatgcgaga ggaagcaacc
     6181 tagagaatgg caagcccacg ttcccgccgc gagacggcgg actactaccg cgatcgacgc
     6241 cggcgcaagg tctgcaactt ctgcgtcgac aaggccgagg cgatcgacta caaggaggtc
     6301 aaccgcctgc gccgatacct gtcggagcgc gccaagatcg agccacgccg caagaccggc
     6361 acctgcgcgc gccaccagcg catgctgacg gtggcgctca agcgcgccag gcacgtcgcg
     6421 ctgctgccgg tggcacccca gcaccttcgc ccctgacagt ccacggctaa cggaggctga
     6481 acgccgatgg acgtcgtcct cctcgtcgtc gcgctggcgg tcatcctgct cggcgccgag
     6541 ctgttcacca acggcatcga gtggttcggg caccggctga acctcgccga gggggcggtc
     6601 ggcagcgtgc tggctgccgt cgccacggcc atgcccgaga ccctcatccc ggtgatcgcg
     6661 atcgtcggac cggcgatctt cggcgggggc tcgccgaact catccgagat cggggtcggg
     6721 gcgatcctgg gcgcgccgtt catgctctca acgctcgcca tgttcgtcac cgggctggcg
     6781 atcgtcatct acacgcgccg ggggcggcgg gagaccgaca tgtcggtcaa cgtgcgggtc
     6841 ctcgggcgcg acgtgatctt cttcctggtg gcctatacga tcgcgatcgc cagcgccttc
     6901 ctgccggctg acctccgcgt cgggcgctgg gctgtcgcga tcggcctggt tgtcctgtac
     6961 gccgtctacg tccgcgccca tttcaccgac cccgccgagg agggggatgg ctccgatctg
     7021 aacaagctcc acctgagccg cctggcggaa aggatgccgg ggcgcatggt cgacttcgac
     7081 gcgctgcccg gcgaggatga cggccacgag catgacgggc ctgtcccgcg catacgcatc
     7141 atcgtgaccc aggtggttgc ggcgttggcg ttgatcgtcc tgggtgcaca ggtcttcgtc
     7201 ggcgcggtgg agcacctctc gagcgccatc gggctggacc ccaggatcct ggcactcgtg
     7261 atcgcgccga tcgcgacgga gctgcccgag aagttcaaca gcgtgatctg ggtccgatcc
     7321 ggcaaggaca cgctggcgat gggcaacatc agcggcgcca tggtcttcca gagctgcctg
     7381 ccgaccgtgc tcggcatcct gttcacggag tggaccttca aggcgagcag cgcgctggca
     7441 ttcgcgtcgg ccggagctgc attcgtctcc acggtcctga tctttggcgt gatgctgcgg
     7501 cgcggccggc tgagtgcatg gtcgctgctg ctcggaggcc cgatctacct cctgtacgtg
     7561 gctgccgccc ttctcctgcc ggccggagcc gccgctgcac actgaggtgg gccatccacg
     7621 ccgagccaac ccgggcacgg gtcgccgtat actgccggcg ccggtaggtc ggcgcccatc
     7681 ggggcgcggc ccggctgact gaggaggtca cgttgctgaa gcaggaaacc gccacggcca
     7741 ccgccgaggc ggaccgcacg gccggtctgt gggcctactt ccacggggac ttcgtgcccc
     7801 tggccgaagc caagatcagc gtgatgacgc atgcgttcaa ctacggcaca gcggtcttcg
     7861 agggggtccg ctgctactgg aacgaggagc aggaacagct ctatgggctg gacctgctgg
     7921 cgcacttcga gcggattcat cggtccgtca gactccttca gctggagctg cgacataccc
     7981 cagaggagct ggtcgggatc accgtcgagc tccttcggcg agatgggttc cgcgaggatg
     8041 cctacatccg cccgatctgc tacaagtcga gcgaggtcat cggcgtccgc ctccacaacc
     8101 tcgagtcgga cctcaccatc ttcgcgatcc cgttcggcaa gtacatcgac accgaggcgg
     8161 ggatccgggc ccaggtcagc acctggcgca ggaccgatga caacgccatc ccggcgcgca
     8221 gcaagatcac cggctcctac gtgaacggcg ccttcgcgaa gagcgaggcg cagatgaacg
     8281 gctatgacga ggcgctgatg ctgacgcagg aggggcacgt cagcgagggg agtgcggaga
     8341 acctgttcat cgtgcgggac ggaacgctga tcacgccccc cggcagcgac aacatcctgg
     8401 aggggatcac ccgcgagcac ctgatgacga tggccggcga cctggggatc ccggtcaagg
     8461 aacgcagcat cgaccgcacc gagctgtaca tggccgacga ggtcttcctg tgcgggacgg
     8521 gcgcccagct ggcgccggtg gtcgaggttg accggcggcc gatcggcaca ggcaaggtgg
     8581 gccccatcac gcatcggctg caccagatct acttcgacgc ggtccgtggc cgctcggacg
     8641 cctatcgcga ctggctcacg ccggtctact agcgaccgct tcgcccgtcg ccgtccgcga
     8701 cggcgacggc gacccagacg agggggccat gtccgaggcg cgaacacagg agcgctatct
     8761 ctggctgcag cgcctgctcg agatggtccc cggaacggtc agctgggcgg tgatcatcgg
     8821 gccgatctgg ctcagcttca gctacccgtg gctcgtggca tatttcgtgc tcagcttcga
     8881 cttctactgg ctctgccgcg ccatctggtt cgccgggtcg gtcatcgtgg cccaccgccg
     8941 catccgcgac gtgctcgcga ccgactggaa cgcgcgcctc gagtccctgg acgatcccga
     9001 catcccggag tggcggtcgt tcacgcacct ggccctgatc ccgacctaca cagagtcgct
     9061 ggagaccctg cgccatacgg tccgcgccct ggccgaggcc gactgggcga ccgatcgaaa
     9121 gatctgcgcg atcatcacgc gcgagaccga cgcaaccggg atcgccaacg tcgaggccct
     9181 gcgcggcgag ttcggggccg ccttcgccga cttcatccac atcctcgacc cgcttgagcc
     9241 ggggatcgtg gtcggcaaga gcagcgccat ggcgttcggc ggtcgctggc tgtatcggct
     9301 cctggttcgc gagcggggga tggacccgag cctgctgctg gtcaccgatc tcgacgccga
     9361 ctaccgcgtc cacccggagt acttcaacta cctgacctgg atgcacgtca gcgatccgaa
     9421 tcgcgacacc cagctctacc agccggttcc ctacttccac aacaacgtct ggcaggctcc
     9481 gctgctgacg cgccttttcg ccgcggtgct gacccagctc cagatgtggc ggagcgtcct
     9541 tcccgagaag ctgcagagct tcggcagcta ttcgacgacc ctgcacctgg tgcacgaggt
     9601 cgggtactgg gccaccgatg cgatccccga ggacagccgc ttctactgga agagctattt
     9661 cacctacggc gatcgcttcc gggcggtccc gctcttcatc ccgatctacg gggacgcggt
     9721 gcgcgccaag acctattggc gctcgctggc cacccagtac ctgcaggccc gccgctgggc
     9781 ctggggcgtc accgaccttc cgtacgtggt gcagaacgcg atcgcgcacc ccgagatccc
     9841 gctctcgtcg cgggcatggc gcgtcctcaa cctgttcgcc gagcacatca actgggccgt
     9901 cgcgcctttc gtggtggcct tcggcgcgac cgtgccgctc atcgtcaacc cggcgttcgc
     9961 ccgcacgacc cttgggcaga acctgccgat ctacgcctcg agcatgctgt cggtcgccct
    10021 cctggcgctg ggagagctga tctggatcga gcaccggatc gtcccgccct tccccgccgc
    10081 gtgggggcgg ctgcgcagga tgctcgtgta cgtcgggtgg ctcgggcttc ccttcgtcgg
    10141 tatcttcttc accaacctgc ccgcactcga cgcgcagact cgcctgctga cggggcgcta
    10201 cctggagtat cgcgttaccg aaaaggtctg accgatgtcc gagctgacga cccagatcac
    10261 atccgtgcga cttgcccgcg accgggcgcg gctcttcgcg gcgccggtgc tgttcctgct
    10321 ggtcgcggcg gcagccgtgg ccggcgggct gctgctcggc ggcccagggg gcatctcgct
    10381 tgtcgcggtc ggggtgctgg ccattgtcgt cgccgcctac ctgacggcac tcgtctcctc
    10441 ctatcgcctg ctcgtcgagc cgggggtcct ccgcctgcaa tggctcggcg gcgagcgtcc
    10501 ctaccggctg gtgcagggga aggtgacccg ggttgggatc agcggcgaga acgctgccgc
    10561 gctccgtccc cgtttcgggg ccctggggtg ggctgttggg ccggcgctgc tgcggggcga
    10621 gccgctcgag ctgatccgcc tctcgaagcg cccccctctg atcgtggcgc cgacggaccg
    10681 tgggcgcctc gcgatcgccg ccgcggtgga gagcgagctg ctggaagcga tgacccacgc
    10741 cgttcacctg caggagcgcc tcgacgaggt tgcctcgcga cgcgccgccc cttcgcccgt
    10801 cgaggtcgcg cccaggccgt cggcgcccgc cgcgccgcgg gtcctgaccg ggatcgagcg
    10861 gacgctcatc gagcagcggc ttgccgcgga gcgcgccgcg gccctggccg ccgccgaagc
    10921 ggaacgagcg ggcgccgcgg cggcgttcgt gcccgaggca ccgctcgtgc cggaggcggt
    10981 gccggcacct ggcccgcgcg aggcgccacc ggctgccgag gcgccggctg cggagcgaag
    11041 ggtcgcccgc ctgcgacgac gcggccaatg gcaacgtccc agctgggtgg cggtgcccgg
    11101 acctgtcagc gtggtgtcgg ccctcccgat cgcgctcccg ctgatcggcg ccggcatcgc
    11161 ctggattgcc gttaccgtga ccggccggcc ggcgttgccc gtcgacgagg cacgaatcct
    11221 gtcgacggcg ctggtgctgg tcgggccgct cggcgcgctg ggcgggctga tcgcaaggac
    11281 gtggtacccg cgcatcggcg gactggtgat ggtcagcagc gtggcggcgc tggccatgct
    11341 gacgcggacg atcctggcct agcgcgttga caaggcgggc gccgcactgc gacgatccgc
    11401 gcaatcgaac acggcgatga cggagccagc gtccgcgtcg acggtggagc cagcagcgag
    11461 cccggacggt gtgagcgggc ggcttcgaga cgacggccga tccctcccga gccggaggcg
    11521 ccaagcggca ggtgccgtgc taatgcctcc cgggccagcc cccgatagcg aggcgcgacg
    11581 aagaggatcg aggtcgatcg gcgacatcgg tcaagcgcgg gtggtaccgc gggaagctgc
    11641 cttcccgtcc cgggatccgg gacgggtttc ctgttgaatg gagcgatcgt gttcaaaccg
    11701 gtatcgggca agctggacgt tccaggcgtc gagcgcgagc agatcgaggt ctggcgcgag
    11761 cgccggacga tggagcgcta cctggctcgc aacgcccaat cggagcggcg ctggagcttc
    11821 ctggacggcc cgatcacggc caacggcccg atgggcgtgc accatgcctg ggggcgcacc
    11881 tacaaggacc tctaccagcg ctaccacacc atgctcggcg agcggcagcg ctaccagaac
    11941 gggttcgact gccagggcct gtggatcgag atcgacatcg agaggcagct cggcttcaac
    12001 aacaagcggc agatcgagga gttcgggatc gaccgcttcg tggagctgtg caaggcacgg
    12061 gtcgacacca tggccgccct ccagaccgag cagagcaagc ggctcggcat gtggatggac
    12121 tgggagcaca gctacttcac caacagcgac gagaacaact acacgatctg ggccttcctg
    12181 aaggagtgcc actcccgcgg cctgatctac aagggccacg acgtgatgcc gtggtgcccg
    12241 cgctgcggga ccgggctctc gaacatggag atcgcgaccg agggctaccg ggagatccgg
    12301 cacctgtcgc tgaccgtacg gctgccgatc accagccccg gccacgaggg cgaggacctg
    12361 ctggtgtgga ccacgacccc gtggacgctg tcgagcaacg tggccgccgc ggtccatccg
    12421 gacctcacct acgtgctggt cgaggacgcc gacgggcggc gctcgtggat ctcgcacggc
    12481 tcgcagcccc gggtcatgtc ggatggcacg gtcgtccgac aggtgaccgg tcgcgagctg
    12541 gtcggccttg cgtattctgg gccgttcgac gagctgccgg tccagtcggg ggtcgagcat
    12601 cgggtgatcg aatggacgga ggtgtcggac gaggagggga ccggcatcgt ccacatcgcc
    12661 ccgggctgcg ggcaggagga cttccacctg gccaagcgtg acgggctggc ggtgctcgac
    12721 ccgatcgacg agttcggcat cttccgcgac ggctacggct ggcagacggg acgctttgct
    12781 ggcgccaccg aggatcaggc caccgatgtc gccaccgacg tggcggccga cctcgagcgc
    12841 aagggcctgc tggtgagtcg cgagcgctac ctgcaccact acccggtctg ctggcgctgc
    12901 gggacgcagc tcgtcttccg gctcgtcgac gaatggttca tcgccatgga cccgctccgc
    12961 cagccgatcg gcgccgcgac gcgtcagatc acctggcttc catccgggat cggcctggag
    13021 gagcgcgagc tggactggct gcgaaacatg agcgactgga tgatcagcaa gaagcgctac
    13081 tacgggctgg cgcttccgat ctgggaatgc gtcgactgcg ccgcctggga ggtgatcggg
    13141 tcgcgcgacg agctcaaaca gcgggcggtg gccggctggg aggcgttcga cgggcattcg
    13201 cctcacaagc cgttcatcga ccaggtcgag atcgactgcc ggtcgtgcgg cgggcgctcg
    13261 cgacggatcg gcgacgtcgg caacccgtgg ctggacgccg ggatcgtgag cttcagcacg
    13321 ctgcgatgga acaccgatcg cgactactgg gcggagtggt tccccgccga cttcgtcgtc
    13381 gagggctttc ccggccagtt ccgcaactgg ttctatgccc tgctgacgat gtcgaccgtg
    13441 ctggcggatg ggccgatgac ccggacgatc ttcggctacg ccacggtcct cgacgagcat
    13501 ggcgaggaga tgcacaagag caaggggaat gccatcccct tcgacgaggc ggcggagcag
    13561 atcggcgccg acgtgatgcg ctggatgtac tcggccgcca acccggccgt gaacctgaac
    13621 ttcgggtacg gccccggcca cgaggtcgtg cgtcggttct tccttcccct ctggaacacc
    13681 tacggattcc tcgtcaccta cgcccggctc gatggctgga cgcccggcga gagtgacgat
    13741 gggatggagt cggcacgcac gctgctggac cgatggatcc tttcccgcct ggatgcggtg
    13801 gtgggcgacg tccgccgcgc gcttgacgca tacgacgccg cgcgagccgc gcgagggatc
    13861 gagggcttcg tcgaggacct ctccaactgg tacgtcaggc gaaaccgccg gcgcttctgg
    13921 aagggcgagc ttgacgacga caagcgcgcg gcgtacgcca ccctgcacga ggtgctggcc
    13981 acggtctcac gactgctggc cccgttcgta ccgcacctgg ccgatgcgat ctggggcaac
    14041 ctggtggcgg ccgtcgatcc gacagcctcc gacagcgtcc atctcaccga ctaccccgat
    14101 cgcatcctcg gccgagcgca gccggagatc gacgccgcgg tgtcgttggc gcgcgaggtc
    14161 gtctcgctgg gccgcaccgc ccgggctgcg tcggggctga agacacggca gccacttgcc
    14221 acggtccggg tcaagctgcc ggcgtcggcc aacgggcagc tgagccccga tccgaccacc
    14281 gccgtggagc tggaggccga gatcctcgaa gagctgaacg ccaagtcgct ggaggtgctc
    14341 accgacgcct ccgagctggt ggagcggacc ctgtacccgc tcctgcgcgt gatcggcccg
    14401 cgccatggcg ccgacgtggc acggatcatg gccggggcca ggtccggtga ctggaagctg
    14461 ctgaacgacg gaggagcgga ggcgggagga gtctcgctcg cctccgacga gttcgagctg
    14521 accgcacgtg cccgacccgg ccatgaggtg gccgaggcgg gtgacgtgct ggtggcgctc
    14581 gatacgcagg tgacccccga gctgctggcc gaggggcttg ctcgtgaggt cgcgcatcgg
    14641 ctccaggggc tgcgcaagac ggccggcctg gaggtcagcg atcgcgtcgc cgcatcgatc
    14701 gacggcgagc ccgctctcat cgcgcagctg gtcccgcacc gcgactggct ggccgaggag
    14761 atcctggccg tcacgctcga cctcggcggc gagacggacc tgggcgtatc ggcggccttc
    14821 gaggaggttg gcatgcccga cgggaggctg cgactgggcg tgcgccgcgc ctgacgcggc
    14881 gcatcgcgta ccatcggcaa ggtgagcagc gaggagccga cgaacggcgg attcaccagg
    14941 gccgtcatcc tcctgctgac cgcactggcc atcctggtcg ccgatcaggt cacgaaggcg
    15001 ctggtggttg cgaacctggc gctcggggag aaggcgcgcc tgctggggga cctggtgcag
    15061 gtctggcatg cgcagaaccg gggagcggca ttcagcctct tccagggcgg aacggtcctc
    15121 ttcctgatcg tctccgtcct ctcgatcggc ttggtcgcct acttccaccg cagcctccgc
    15181 gaccgcaccc cgtggctgca tgtcgtgctc ggcatcgtcc tgggcggcac gctcggcaac
    15241 ttcctcgacc ggctgcgcca ggggtacgtc accgactggc tctcggtcgg catcggcgac
    15301 acgcgctggc cgaccttcaa cgtcgccgat tcgtccgtcg tggtcggcat cggcatcctg
    15361 gtcgtatacc tcttcgtgac cagccccgac cgacgccagg cgaccgcatg agcgacgtgg
    15421 ccacggccgg tggccgcgtg gtgaccgggg cagccgtgac cggtcgattc gacctggcgg
    15481 tcagcgcggt ggcggagatc agccgggcgc atgcgcagcg gctcatctcg gacggccgcg
    15541 cactggtcga cggccggcgg gcgcgtgcgt cagatcggct gcgcggtggc gagcagatcc
    15601 gcgtcgaact gtccgcgccc cccgatccga cgctgcgcgg ggagtcgatc ccgttgcgga
    15661 tcgcctacga ggacgggtcg atgctgatcg tcgacaagga cgccgggctg gtcgtgcatc
    15721 ccagccacgg gcactccacc gggaccctcg tccatgcgct cgtcggccga gcggaggagc
    15781 gcggcgagga gctcggatcg atcgccggcg tcgggcggcc cgggatcgtg catcgactcg
    15841 acaagcagac ctcggggctg atcgtcgtcg cgaagtcgga tgcggcgcag gcgagcctga
    15901 tgcagcagtt cggcgcccgc accatcgaga aggagtacct ggcgctggtt cgcggaacac
    15961 cgcccgcgcc gcgcggccgg atcgaggccc cggtcgggcg cgatccacgg gaccggcagc
    16021 ggatggcggt ggtggctggc ggccgcgagg cagtgactga gtatgagacg ctcggcagcg
    16081 ccgacggcca ttcgctcctg gcgctgcacc cgctcaccgg gaggacccac cagctgcggg
    16141 cccatctcgc ctacctcggc ctgccgatcg ccggcgacac gcgctacggc ggggggatgg
    16201 ggccgggtgg cctgcagcga cagttcctgc acgccgctcg cctgactctc gaccgtccga
    16261 gcgacgggcg gcggctcacc gcctggagcg agctgccgcc ggacctgtcc gcctcgctgg
    16321 cggcgagcga gatcgtgacc gatcggctgc cggtcgggat cggcgcggcc ctggtggagg
    16381 atcagggttg accggcaatg gtccggccga caagcccgcc aagcgcctcg cctttcccaa
    16441 cccgctcctg gtcgtggttt ccgggccttc cggggtcggc aagagcacga tcgtagccga
    16501 gctgtcccgg cggcacccac aggtagtccc gatcgtcacg gcaaccactc gggagcagcg
    16561 cgagggggag ctcgacaagg ttcactacca cttcctgagc cccgacgagt ttcagcagct
    16621 cagggaccgc aaaggcctcc tcgagcaggc cgtcgtccac gaccactggt atggcacccc
    16681 ggtcgaccag gtgcggggca tcctggcggc aggacgcgac gcaatcctga ccatcgatcc
    16741 gcagggggcg cgatcggttc gaacgctggt gccggatgcg ctcctgatct tcatcatgcc
    16801 gccgtcgatc gaggacctcg accagcggct cgcgcggcga gggtcggagg atggggacga
    16861 gcgtgcgaag cgccgacgca acgctgaggc ggagatggcc gcggccggcg actacgacta
    16921 cgccatcgtc aaccagaccg atcaccccga tgacgcggtc gagcagatct gggagatcat
    16981 ccaggccgaa gcccggcgcg acccaccgcg gcgcgtccag gtctgagcga ggtggccgag
    17041 gtggccgccg gggagacggt gcgcgtcgcc gtcgaggcgc ttgccgatcg gccggagcgc
    17101 gcgttcagct atcgcctccc acctgagctc gggacggcgg cggccggctc gctgctgctg
    17161 gtgccctatg gccgccggct ggcgctcggc tacctgctcc ccgggactga ggagcccgag
    17221 ccggggatcg agctgcgatc ggtcgaggcg gtcgtctcgg cgccgatgct gacccccgat
    17281 ctgctcgccc tggccgagga gatcgccgtc tactactgcg ccccgcttgg cgggaccatc
    17341 ggcgcgatgc tcccgccagg gctcgagtcg cgcctggagc gacgctggac gacgccggag
    17401 gtcagccttc tgccggaccc gctgcacgcg atgctgtcgg gttccgatga gatcagcgac
    17461 gccatgttga agcgctcgct cggcccgcgc tggagccccg tcgccgaccg gctgcgccgc
    17521 tccggggcgc tggtcgcgcg ctggtcgctg cgaccgccgg agctgaaggc gcgtcgcgtg
    17581 cggaccc
//
LOCUS       DATCQR010000138         2943 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_100313, whole genome shotgun sequence.
ACCESSION   DATCQR010000138 DATCQR010000000
VERSION     DATCQR010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2943
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100313"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            127..2343
                     /locus_tag="VIN32_04695"
     CDS             127..2343
                     /locus_tag="VIN32_04695"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007290246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEY8921910.1"
                     /translation="MDLARLRAAPAAVAKRISRNAEPAASTPTIESVIAEARRHRPSI
                     NVGLIERAHQVATTAHEGQLRASGEPYITHPVAVAYELAALQMDPESIAAALLHDVPE
                     DTDTTIADLEKRFGREVARLVDGVTKLSRFGSARSMEEQQAENIRKMFLAMAEDVRVV
                     IIKLADRLHNMRTLGALPPDKQVRIARQTMEIYAPLAHRLGIWQWKRELEDLSFKHAE
                     PETFRKMVEMLADRRKERESFVNKSIGILRKELAKVGIEAEISGRPKHLYSVYKKMER
                     KGAEFSEIYDLHAVRVIVDEIKDTYAALGVVHSIWRPIPGQFDDYIAMPKANLYQSLH
                     TAVIGPDAKPLEVQIRTRAMHEVAEAGIAAHWRYKEGSRGDRRYDEKLAWVRQLIDWQ
                     REVVDATEFVEGLKLDIFQDQVFVFTPKGDVKDLPSGATPLDFAYRIHTDVGHRTIGS
                     KVNNRLVPLDYRLHNGDIVEIITTKAAHGPSRDWMNIVRTNQAREKIRAWFKRQQRDE
                     NIAQGKEILDRELRRLAHETLVSIDADKLLELAAHAHYKELDDFYAAIGYGAVSPASV
                     VGKLEIHDDAAIALPEVAPPSGVPSASGIRVKGVGDLLVRFAKCCSPIPGDPITGYVT
                     RGKGVTVHRSNCRSVLSERDIERLIDVDWEAVDLATYPITIRILAVDRPGLLNEITTV
                     VAEDKVNIVAASIGTHEDGTATITATLKVTSLQQLSKVLTRIERLRDVTSVSREAR"
     gene            complement(2392..>2943)
                     /locus_tag="VIN32_04700"
     CDS             complement(2392..>2943)
                     /locus_tag="VIN32_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921911.1"
                     /translation="RMDPISYVGRIIGAGSFKEMSSANAANIWGLIAGYKHPDGGLVY
                     VGGVLLLLGLGAALLPLLRRQDLAMILAVGAFVIFAFYFLPTRVHERYLFPAMAVLAP
                     LAAANWSVLGAYVLMTAGFAASMLYALVDTTPFTFWPWLEDLVILPVARIWISLTLIA
                     TAATLVLLLVQPVFRSTPMREGE"
BASE COUNT          565 a          986 c          982 g          410 t
ORIGIN      
        1 aagtagaggc gtgagccgcg cggtccgaag cgagttgcgg gagccgagcg aggacggtcc
       61 ggctcacgcc acgactccat cgggccatcc atcacctccc gaccgaggtg ctaccctctt
      121 gccaccatgg acctggcacg cttgcgggcg gccccggccg ccgtggcgaa gcgcatctcg
      181 cgaaatgccg agccagcggc cagcacgccc accatcgagt ccgtcatcgc ggaggcgcga
      241 cgacatcggc cgagcatcaa tgtcggcctg atcgagcgtg cccaccaggt ggcaacgacg
      301 gcgcatgaag gccagctcag ggcgtcgggc gagccgtaca tcacccatcc ggtggcggtc
      361 gcgtacgagt tggcggcgct ccagatggat cccgagagca tcgccgccgc gctcctccac
      421 gacgtgccgg aggacaccga cacgaccatc gctgacctcg agaagcgatt cgggcgcgag
      481 gtggcgcgac tggtcgatgg ggtcaccaag ctcagcaggt tcggctcggc gcgctccatg
      541 gaggagcagc aggctgagaa catccggaag atgttcctgg ccatggccga ggacgtgcgg
      601 gtggtcatca tcaagctggc agaccggctt cacaacatgc gcaccctggg cgcgctgccg
      661 cccgacaagc aggtgcgcat cgcgcggcag acgatggaga tctacgcgcc gctggcccat
      721 cggctgggaa tctggcaatg gaagagggag ctcgaggacc tctcgttcaa gcacgccgag
      781 cccgagacct tccgcaagat ggtcgagatg ctggccgacc ggcgcaagga gcgcgagtcg
      841 ttcgtcaaca agagcatcgg catcctgcgc aaggagctgg ccaaggtcgg gatcgaggcg
      901 gagatcagcg gacgccccaa gcacctttac agcgtctaca agaagatgga gcgcaagggc
      961 gccgaattca gcgagatcta cgacctgcac gccgttcggg tgatcgtgga cgagatcaag
     1021 gatacgtatg ccgccctggg cgtggtgcat tccatctggc gaccgatccc cgggcagttc
     1081 gacgactaca tcgccatgcc caaggccaac ctgtaccagt cgctgcacac cgcggtgatc
     1141 gggcccgatg ccaagccgct cgaggtccag atccggacgc gcgccatgca cgaggtggct
     1201 gaggcgggga tcgcggccca ctggcgctac aaggagggct cgcgcggcga tcgccgctac
     1261 gacgagaagc tggcctgggt gcggcagctc atcgactggc agcgcgaggt ggtggacgcc
     1321 accgagttcg tggaggggct caagctcgac atcttccagg accaggtctt cgtcttcacc
     1381 cccaagggcg acgtcaagga cctgccctcc ggcgcaaccc cgctcgactt tgcctaccgg
     1441 atccataccg atgtcggtca ccgcaccatc ggctccaagg tcaacaaccg attggtgccg
     1501 ctcgactacc ggctgcacaa cggcgacatc gtcgagatca tcaccaccaa ggccgcgcac
     1561 ggaccttcgc gcgactggat gaacatcgtc cgcaccaacc aggctcgcga gaagatccgt
     1621 gcctggttca agcgccagca gcgcgacgag aacatcgcgc agggcaagga gatcctcgac
     1681 cgcgagcttc ggcgcctggc gcacgagacg ctggtcagca tcgatgccga caagctgctg
     1741 gagcttgccg cgcacgcgca ctacaaggag ctggacgact tctacgccgc catcggctac
     1801 ggggcggtct cgccggcgag cgtggtcggc aagctcgaga tccacgatga cgcggcgatc
     1861 gccctgccgg aggtggcgcc gccgtccggc gttccctccg ccagcggcat ccgggtcaag
     1921 ggcgtcggcg acctgctggt gcgcttcgcc aagtgctgct cgccgatccc gggcgatccg
     1981 atcaccgggt acgtgactcg tggcaagggc gtcaccgtgc accgatccaa ctgccgctcg
     2041 gtcctgtcgg agcgcgacat cgagcgcctg atcgacgtcg actgggaggc ggtcgacctg
     2101 gcgacctacc cgatcaccat ccggatcctg gcggtcgatc gcccggggct gctcaacgag
     2161 atcaccaccg tggtggccga ggacaaggtg aacatcgtgg cggcgtcgat cggcactcat
     2221 gaggatggca ccgccaccat caccgcgacg ctcaaggtca cctcgctcca gcagctttcc
     2281 aaggtgctga ctcgcatcga gcggctgcgc gacgtgacca gcgtcagccg cgaggcgcgc
     2341 tagggagctc gcacccgccg tcacggacag tcccgccggc gcgggatcgc cctactcgcc
     2401 ctcgcgcatc ggcgttgacc tgaacacagg ctgaaccagg agcagaacca gcgtcgcagc
     2461 cgtggcgatc agtgtcagcg agatccagat gcgggcgacc ggcaggatga cgaggtcctc
     2521 aagccacggc cagaaggtga acggcgtggt gtcaaccagg gcgtagagca ttgacgcggc
     2581 aaagccggcg gtcatcagga cgtacgcccc caggacgctc cagttggcgg cggccaaggg
     2641 ggccagcacg gccatggccg ggaacaggta gcgctcgtgc acgcgcgttg gcaggaagta
     2701 gaaggcgaag atcacgaacg cgccgacggc caggatcatc gccaggtcct gccgccggag
     2761 gagcggcagc agggcggcgc ccaggcccag cagcagcagg acgccgccca cgtatacgag
     2821 tccgccatcg gggtgcttgt aaccagcgat caggccccag atgttcgcag cgttggccga
     2881 cgacatctcc ttgaagctcc ccgcaccgat gatccggcca acgtacgaga tcgggtccat
     2941 gcg
//
LOCUS       DATCQR010000139        11543 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_116721, whole genome shotgun sequence.
ACCESSION   DATCQR010000139 DATCQR010000000
VERSION     DATCQR010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 11543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11543
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116721"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="VIN32_04705"
     CDS             <1..189
                     /locus_tag="VIN32_04705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921912.1"
                     /translation="RRLLSSLYQQVRATGHSLGVAAATRAATGPEHGGSVCRHCGFES
                     GTDAFYCPKCGMRMGGQA"
     gene            203..544
                     /locus_tag="VIN32_04710"
     CDS             203..544
                     /locus_tag="VIN32_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HEY8921913.1"
                     /translation="MPTYDYQCRSCGFITEVVHSMLEDGPTECERCGGQLRRVFHPTG
                     IIFKGGGFYKTDSRSASSGSAAGSGRGGSGESGDAGAKVPSSSDAGGGDKGSAPKSAG
                     DGASKGDGGGG"
     gene            complement(541..1473)
                     /locus_tag="VIN32_04715"
     CDS             complement(541..1473)
                     /locus_tag="VIN32_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921914.1"
                     /translation="MTTMLDTLTESFCERCGTRYEFGAPEKLTPIRKTRGMISGLKNY
                     IMSQDSLSDSVNDAMRLQAERLAARQLDAFHTSFSFCINCRQYTCSNCWNGPAGLCQS
                     CEPLSDVEPTAHWSGDMQAATAWPASDLTEPLLLTSLEVAEPELVVVEAEVEAEPEPI
                     EVEAEVEPEPVVVEAEVEPEPEPTPIKPVRPIRETFVHLPRPTPRPRDPASVAADSEA
                     LAARRAQLEVLGLGDPGEGPVGPERKVLPYRSSGAATPVARLAGGAFWDASAREVASA
                     ISQIGVRSCAQCELSLSATARFCRRCGHPQTQSA"
     gene            1628..2383
                     /locus_tag="VIN32_04720"
     CDS             1628..2383
                     /locus_tag="VIN32_04720"
                     /inference="COORDINATES: protein motif:HMM:NF023818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3662 and FHA domain-containing protein"
                     /protein_id="HEY8921915.1"
                     /translation="MGIIARLESFLERLFEAPAGRLGATIQPVLLTKRIERAMDTGKA
                     FGAAGVIVPNRYDLHLHPADFASFEPYRGSLEDDLAHGAASRARREHYQLVARPTVTL
                     VADPAVPRGDVRVAANVVDVAGEPLPEPMPAQPSDQTMVLARSGSAPAPPDSASRAFL
                     LVHTDGAPRVRFDLGGALISIGRASDNDVIVDDPEVSRHHCQLKLQHGAYSLADLGSR
                     NGSWINGQPVSEVALGPGDVIQIGSTEIEFQVA"
     gene            2383..2826
                     /locus_tag="VIN32_04725"
     CDS             2383..2826
                     /locus_tag="VIN32_04725"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEY8921916.1"
                     /translation="MTGEVLRILLLVLQLGFLALLYLILIGFSRALLRDLRSAEVTQM
                     ASQSGIGKLAVVESPADEPALGSVIALGPINSIGRDVNGTIFIDDDFVSGTHAMLTFR
                     GRSWFIEDQGSTNGTYVNGHRIDRPVALSFGDELTIGRVRMRLER"
     gene            2826..4166
                     /locus_tag="VIN32_04730"
     CDS             2826..4166
                     /locus_tag="VIN32_04730"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEY8921917.1"
                     /translation="MHAVPRPLELRLLLPLVILVPLGFAVTHIAQTGTPDVGPMGVAI
                     GYVGLMLGAHLALRLVGNRGDELLLPCVATIGGIGIVMLNRLPQELLGTDVFGVRFGM
                     ATTQLLWFGVGISAMVLLAGRFRDDGVLRHYKYTWALAGILLLVVTFLFGREVNGARL
                     WIFLGPIGFQPGEAIKVVLVIFIAGYLAEKRTLLAGASARIGPFSVPPLPYLLPMIAI
                     FVIVMLVVVVSRDLGTALLFMGIFLTMLFVATGRRSYVLLGILLFIAGSFVAYNLFGH
                     VRIRVDNWIDPFVDPSNRGYQTVQALYAFGRGGLFGEGLGQGLPTVAGHLPIPALPTD
                     FIFAAVAEELGLIGAFALLALVMCLVFRGLRTAMLARDDFSAMLAVGLTVSLGLQTLI
                     IAAGNVKLVPLTGITFPFVSYGGSSLLACFVIVGLLLAISHRSVVDAQIEGRGQ"
     gene            4163..5710
                     /locus_tag="VIN32_04735"
     CDS             4163..5710
                     /locus_tag="VIN32_04735"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEY8921918.1"
                     /translation="MIATNIRRLSLYLLLAFASVSGALVWWQVLDAQALATRQDNPQV
                     IATRRSLPRGSIFDSQGRLLASSLVTEGISRRTYTDPAFTHLLGYASLRFGATGVERA
                     WDDLLTGRTDPNPLRDLLNDILARKPEPHDLTLTIDQRLQDFAAAQLGANRGALVAID
                     PRSGAVLAMVSNPTYDATGFSGDPATAQAAFDAVAGAADNPFLDRSRQGHYTPGSIMK
                     VLTASAALDAGAITPQTTFPDQPREEVDGFVVNGFTIREHDLAPVAPALWDLSPALQV
                     SSNIYFAHVGLELGAERYLDYARRFGFCQALRIGTDARGLPVDPSYVSAQADGGCAPF
                     RDEVELASAAFGQAAVSVTPMQMALMAATIANQGAVPQPYVVRDLRSHAATPTAGPTD
                     SVLETYGGGRGNPAISSGVADQVRRAMIDAVVGPIGKLYAGAGDVTRFGISGVQTAGK
                     TGTAERGPGLKPHSWFIGFAPAQDGALPSIAIAVIVEGGGSGSGHAAPIGGAVMAEWL
                     KLIGTGG"
     gene            5805..6767
                     /locus_tag="VIN32_04740"
     CDS             5805..6767
                     /locus_tag="VIN32_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEY8921919.1"
                     /translation="MKSVKESAERVVANVERVIVGKHVEVRIALVALLCEGHILIEDV
                     PGVGKTMLAKALSRSIGCTFRRIQFTPDLLPSDVTGLSIFNQKTQEFEFRPGPIMAQV
                     VLADEINRATPKTQSALLECMEERQATIDGVSHPMPSPFLVIATQNPIEYEGTFALPE
                     AQLDRFMLRLRLGYPKAMEEIVILDEQKRKHPIDQLQQVLDIDELREMQAAVKEIYVD
                     QAVAEYIVRLTGATREHADVYLGASPRGSINLYRAGQALAALDGRDYVIPDDVKQLAV
                     AVLGHRLIVKSQASLREVDPDGIVREILGQVPIAEASSATAGTR"
     gene            6776..8032
                     /locus_tag="VIN32_04745"
     CDS             6776..8032
                     /locus_tag="VIN32_04745"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEY8921920.1"
                     /translation="MGLSRQLRAVFFGTALVVAAFSTGIDELFFLVYLLATLLLASRW
                     YARRGLRGLRAGYHVLNPRSQVGDTLEAVYRVDNDSGWTKPWVEVANESTLPAPLPGR
                     VVGVKARGSRQWLAKVVLLRRGSYRLGALRVRTGDPFGLFSSEMVVGRPTWIMVFPRV
                     HPLPHWRLPPSPVDGSTPSRRRYEAATPLVSTVRPYVYGDAINRIHWLSSVRHGELIV
                     KEFDLEQAADLWLLLDLQRAAHAGPAESSSVETAVSAAASIAVHTLAENRAVGITVSS
                     RRQQHLNPDRGTRVEQKILNLLANVQADGSQPLAEVVVSTLPQLRRGMTLCIVTGSTD
                     REWVRALAALRRRGIGTIVVLLDRFSFAGRDDDESGAELAAIRHALAEYDIAHHLLRA
                     GDDLSSVLGRRAIGARTPAIRERTRA"
     gene            8025..10316
                     /locus_tag="VIN32_04750"
     CDS             8025..10316
                     /locus_tag="VIN32_04750"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminaseTgpA domain-containing protein"
                     /protein_id="HEY8921921.1"
                     /translation="MPELLRRFLRPREGWGSMALLIVMLLTLVWSLQRAAWVSHMDYL
                     LPVAVVAALLGALLGLTRLSVTVILPLAAIAGAGLVLFTIGAEYFPELNQSSRLILLR
                     GDALDWTRIVVEGGFAPQLSPYAIGLGVLMWVTSFMAAYALYRHHRVLDSILLVGVAL
                     IANMSAILTDLFLYLVLFSLAALLLWVRIALVNREESWRIRRLRENLDVPGQIMRSGV
                     AFIVGSVALAWVLTTVAVAAPLTSVWSNLDGLWSDVRNQFEGAFGGLAGTDSRIQGTS
                     FGSSFRVHGSWSSSDGAVMTVGSTRGYYMRTITYDTYTGHGWSTSDGPNRRVAAGDRI
                     FPGYTPERPLAPSAFQIETVTVEVQRNAGRNVFTPGYPTTAFAPLVVHEPRGLPLLGA
                     LQSAVVLQEGKGYQITAAISTATEAMLAGAGTDYPPEIVATYTGTGGVTQRTADEARR
                     VVNAAGATDPYHEARALADYLRTDRFTYQTVASLPSDPNRDLVDFFLFDPAGQVGYCE
                     YFATTMAVMARTLGLPARVAVGYAPGERLQPGIYQYRERNAHAWAEIFFPGYGWQIFE
                     ATKSIAPVIRAPGAGVVPPVPPANGQIDPGRRVPEDQKTGTISGLPSFEPVEGGFAPG
                     GQRPTDEARTGNAWLIVVLLMLLLAVVAWRLLRGRYRFRFLAPGDRQWQRLAFAADRA
                     GVAQRPSETVYEYAGWLEGQLPGRRVEIHDIADGKVWQSYSGRSMSSEVIARLERAWG
                     RLQLPLLWLALRRRLRSLLGSGS"
     gene            complement(10319..>11543)
                     /locus_tag="VIN32_04755"
     CDS             complement(10319..>11543)
                     /locus_tag="VIN32_04755"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HEY8921922.1"
                     /translation="GLRGGELRGIGPFHGPIMMPAMEQNLRVPGPTPLPPEALEAQSA
                     PMIDHRGTEFGELLREISTGLAELIGTRGEVLLLTGSGSGALEAAVVNTLSPGDRVLA
                     VTIGSFGDRFAKIATAFGAGVERFEVAWGEAADPGALAQHLAANPAYRAVLLTHNETS
                     TGVTNPLRELAEAVRKAPGDPLVLVDGISGLGAMPFEMDGWGIDLVVSASQKAWMGSP
                     GIAIAALGDRAWSANETARMPRAYWDLAEARMWAEKGQTPWTPAVSVLFGLRVGVRRL
                     VAEGRERTWARHEAIAAAVQAGLEALGLRLAAAPAVRSSTVTAAWLPEGLEWAPFNAA
                     MRARGLIVAGGQGQWAGRILRFGHMGGVGIDEMTDAVRIMGETLAEHGRQADAPAAVR
                     VMREVFEARQAAPVG"
BASE COUNT         1616 a         4068 c         3996 g         1863 t
ORIGIN      
        1 cgccgcctgc tcagctctct ctatcagcag gttcgagcca cgggccacag ccttggcgtg
       61 gcggccgcca cccgcgcagc gactggtcct gagcacggcg gctcggtctg caggcactgc
      121 ggcttcgagt cggggaccga cgccttctac tgccccaagt gcggcatgcg catgggtggt
      181 caggcctgag cctgagctag ccgtgccaac ctacgactac cagtgccgca gctgcggctt
      241 catcaccgag gtggtccact cgatgctcga ggatggaccg accgagtgtg agcgctgcgg
      301 cggccagctc cgacgcgtct tccatcccac ggggatcatc ttcaaggggg gcgggttcta
      361 caagaccgat tcgcgctcgg catcctcagg cagcgccgcg ggctcgggcc gcggtggctc
      421 aggcgaatcg ggagatgcgg gcgccaaggt tccctcgtcg agcgatgccg gcggcggaga
      481 caagggctcc gcacccaagt cggctggcga tggcgccagc aagggtgacg gcgggggcgg
      541 ctaggccgat tgggtctggg ggtgaccgca acggcggcag aagcgtgccg ttgccgagag
      601 cgacagctcg cactgcgcgc agctacggac accgatctgg ctgatcgcgc tggccacctc
      661 gcgagccgag gcgtcccaga acgcgccgcc ggcaaggcgg gcgaccggtg tcgccgctcc
      721 gctcgatcgg tacgggagga cctttcgctc cggaccgacc gggccctctc ctggatcgcc
      781 gagccccagg acctcgagct gtgcgcgtcg ggcggcgagc gcctcgctgt cggcggcgac
      841 cgatgcgggg tcgcgtggcc ggggagtggg gcgtggaagg tgaacgaagg tctcgcggat
      901 cggccgcaca ggcttgatgg gcgtcggctc cggctcaggc tcgacctcgg cctcgaccac
      961 gaccggctcg ggctcgacct cggcctcgac ctcgatcggc tccggctcgg cttcgacctc
     1021 ggcctcgacc acgaccagct cgggctcagc cacttcgagg ctcgtgagga ggagcggctc
     1081 ggtcaggtcg ctggccggcc aggcggtggc ggcctgcatg tccccggacc agtgcgccgt
     1141 cggctcgacg tcggagaggg gttcgcagct ctggcacagg ccggccggcc cgttccagca
     1201 gttggagcag gtgtactggc ggcagttgat gcagaagctg aacgaggtgt ggaaggcatc
     1261 gagctggcgg gccgccagcc tctccgcctg caagcgcatg gcgtcgttga cagagtcgga
     1321 cagggagtcc tggctcatga tgtagttctt gaggcccgag atcatcccgc gcgtcttgcg
     1381 gattggggtg agcttctcgg gcgcgccgaa ctcgtagcga gttccgcagc gctcgcagaa
     1441 ggattcggtc agcgtgtcga gcatcgtcgt caaatcggca tccggttcag tgaaaggtac
     1501 tgggactcga gtataccgag ccggggccgc cgctccccac gggcgcattg gtaccttcgc
     1561 gcacgccgtt ggtaccccgc tccccacgct cgccggagcc ggccgcggcc cgtgctagga
     1621 tcgggcgatg ggcatcatcg cgcggctcga gtcttttctc gagcggctct tcgaggcgcc
     1681 ggccggacgc cttggtgcga ccatccagcc ggtattgctg acgaagcgca tcgagcgcgc
     1741 gatggacacc ggcaaggcat tcggtgcggc cggagtcatc gtcccgaacc ggtacgacct
     1801 gcatctgcac ccggccgact tcgcgtcgtt cgagccgtac cgagggtccc tcgaggacga
     1861 cctcgcacac ggcgccgcgt cgcgcgctcg ccgcgagcac taccagctgg tggcgcggcc
     1921 cacggtcacg ctggttgccg atccggccgt gccgcgtggg gatgtccggg tggcggcgaa
     1981 cgtggtggac gtggcgggtg agccgcttcc tgagccgatg cccgcgcagc cgtcggacca
     2041 gacgatggtc ctggcgcgat ccggcagcgc gccggcccct cccgattcgg cgtcccgcgc
     2101 ctttctgctg gtccacaccg acggtgcccc gcgcgtccgc ttcgacctgg gcggcgcgct
     2161 gatcagcatc ggccgtgcca gcgacaacga cgtcatcgtc gacgatccgg aggtgagccg
     2221 ccaccactgc cagctcaagc tccagcacgg cgcgtacagc ctggcagacc tggggagccg
     2281 caacggctcg tggatcaacg gccagcccgt cagcgaggtt gcgctggggc cgggcgacgt
     2341 gatccagatc ggcagcaccg agatcgaatt ccaggtcgcc tgatgaccgg tgaggtcctc
     2401 aggatcctgc tgctggtcct gcagctcggc ttcctcgctc tcctgtacct gatcctgatc
     2461 ggcttctcgc gggccctgct gcgcgacctg cgcagtgcgg aggtgacgca gatggcgagc
     2521 cagtccggga tcggcaagct ggcggtggtc gagtcgcccg ctgacgagcc ggcgctcggc
     2581 agcgtgatcg ccctggggcc gatcaactcc atcggccgtg acgtcaacgg caccatcttc
     2641 atcgacgacg acttcgtgag cggcacgcac gcgatgctga ccttccgcgg ccggagctgg
     2701 ttcatcgagg accaggggag caccaatggg acgtacgtca acggccatcg catcgaccgg
     2761 ccggtggcgc tctcgttcgg cgacgagctg acgatcggcc gggtccggat gcgcctggag
     2821 cgctgatgca cgccgtcccg cgtccactgg agctgcgcct cctgctgcca ctcgtgatcc
     2881 tggtcccgct cggctttgcc gtcacccata tcgcgcagac cggcacgccc gatgtcgggc
     2941 cgatgggggt ggcgatcggc tacgtcggcc tgatgctcgg cgcccacctg gcgctgcgcc
     3001 tggtcggcaa ccgcggggat gagctgctgc tgccgtgcgt ggcgacgatc ggcgggatcg
     3061 gcatcgtcat gctgaatcgc ctgccccagg agctgctcgg caccgatgtc ttcggcgtcc
     3121 ggttcgggat ggcgaccacg cagctgctct ggttcggggt cggcatcagc gcgatggtgc
     3181 tgctggcggg ccgcttccgc gacgacggcg tgctgcggca ctacaagtac acctgggccc
     3241 tggccgggat cctgctgctg gtggtcacct tcctgttcgg gcgcgaggtg aacggggcgc
     3301 gcctgtggat cttcctggga ccgatcggct tccagcccgg cgaggcgatc aaggtggtgc
     3361 tcgtcatctt catcgccggc tatttggccg agaagcggac cctgctggcg ggggccagcg
     3421 cgcgcatcgg cccattcagc gtcccgccgc tgccctacct gctgccgatg atcgcgatct
     3481 tcgtgatcgt catgctcgta gtcgtcgtca gccgagacct gggcacggcg ctcctcttca
     3541 tggggatctt tctgaccatg ctgttcgtgg cgaccggacg gcgcagctat gtcctgctgg
     3601 ggatcctgct cttcatcgcc ggctccttcg tcgcctacaa cctgttcggc cacgtccgga
     3661 tccgggtcga caactggatc gacccgttcg tcgacccgag caaccggggc taccagaccg
     3721 tgcaggcgct ctacgccttc ggtcgcggcg gcctcttcgg ggaggggctc ggccaggggc
     3781 tgccgaccgt cgccgggcac cttccgatcc cggcgctgcc cactgacttc atcttcgccg
     3841 cggtggccga ggagctgggg ctgatcggtg cctttgccct gctggcgctg gtgatgtgcc
     3901 tcgtcttccg gggcctgcgc acggcgatgc tggcgcgcga cgacttcagc gcaatgcttg
     3961 cggtggggct gacggtcagc ctgggcctgc agacgctgat catcgccgcg ggcaacgtga
     4021 agctggtgcc gctgacgggc atcaccttcc cgttcgtcag ctacggcggc tcgagcctgc
     4081 tggcctgctt cgtgatcgtc ggactgctgc tggcgatcag ccaccgatcg gtggtcgatg
     4141 cgcagatcga gggacgcggg caatgatcgc caccaatatc cgccgcctgt cgctgtacct
     4201 gctgcttgcc ttcgccagcg tctccggcgc gctcgtgtgg tggcaggtgc tcgacgccca
     4261 ggcgctcgcc acgcggcagg acaacccgca ggtcatcgcg acgcgccgca gccttccgcg
     4321 cggctcgatc ttcgactcgc agggacgcct gctggcgtcg agcctggtga cggaggggat
     4381 cagccggcgc acctacaccg atccggcgtt cacgcacctg ctgggctacg ccagcctgcg
     4441 gttcggggcg accggcgtgg agcgggcctg ggatgacctg ctcaccggtc gcacggaccc
     4501 gaatccgctg cgcgacctgc tgaacgacat tctcgcccgc aagcccgagc cccacgacct
     4561 cacgctcacg atcgaccagc gcctccagga cttcgccgcc gcgcagctgg gtgccaaccg
     4621 gggagcgctg gtggcgatcg acccgcgcag cggggcggtg ctggcgatgg tctccaaccc
     4681 cacctacgac gcgaccggct tctcgggtga ccccgccacg gcccaggcgg ccttcgacgc
     4741 ggtggcgggg gccgccgaca atccgttcct ggaccgatcc cgccagggcc actacacgcc
     4801 gggctcgatc atgaaggtgc tgaccgcctc ggccgccctc gacgccgggg cgatcacgcc
     4861 tcagaccacc ttcccagacc agccccgcga ggaggtggac gggttcgtgg tgaacggctt
     4921 cacgatccgc gagcacgacc tcgcgcccgt ggcgccggcg ctctgggacc tatctccggc
     4981 gctgcaggtg tcgagcaaca tctacttcgc gcacgtcggg ctggagctcg gcgcggagcg
     5041 ctacctcgac tatgcgcggc ggttcggctt ctgccaggcg ctccgcatcg gtaccgatgc
     5101 ccgcggcctg ccggtcgatc cctcgtacgt ctccgcccag gccgatggcg gctgcgcccc
     5161 gttccgggac gaggtggagc tcgccagcgc cgccttcggg caggcggccg tatccgtcac
     5221 gccgatgcag atggcgctga tggccgcgac gatcgccaac cagggtgccg ttcctcagcc
     5281 ctacgtggtg cgtgacctgc gcagccacgc ggcaacgccc accgccggcc caaccgacag
     5341 cgtgctcgag acgtacggcg gcggacgggg caacccggcc atctcgtcgg gcgtggcgga
     5401 tcaggtgcgg cgcgcgatga tcgacgcggt cgtcggaccg atcggaaagc tctacgcggg
     5461 ggccggggac gttacgcgct tcggcatctc cggtgtccag accgcgggca agaccgggac
     5521 ggcggagcgc ggccctggcc tcaagccaca ctcgtggttc atcggcttcg ctcccgccca
     5581 ggatggcgcc ctcccgtcga tcgcgatcgc ggtcatcgtc gagggcggcg gaagcggctc
     5641 ggggcacgcg gccccgatcg gcggcgccgt gatggccgag tggctgaagc tgattggcac
     5701 gggcggctga agccaggcgc caaagccccg gtggacgacg cgctggggta ttgttcggcc
     5761 cgggcggaca cgcccgctgg ctcgaatcaa ggggagtctc cgcggtgaag agtgtcaagg
     5821 agtcggcgga gcgcgtcgtc gccaatgtcg agcgcgtcat cgtcggcaag cacgtcgaag
     5881 tgcggattgc gctggtggcg ctgctgtgcg agggccacat cctgatcgag gatgtgccgg
     5941 gggtcggcaa gacgatgctg gccaaggcac tcagccgaag catcggctgc accttccggc
     6001 ggatccagtt cacgccggac ctgctgccgt cggacgtcac gggcctctcg atcttcaacc
     6061 agaagacgca ggagttcgag ttccgacccg ggccgatcat ggcgcaggtg gtcctggccg
     6121 atgagatcaa ccgggccacg cccaagaccc agagcgcgct gctcgagtgc atggaggagc
     6181 gccaggcgac gatcgatggc gtcagccacc cgatgccgag cccgttcctg gtgatcgcaa
     6241 cccagaaccc gatcgagtac gagggcacct tcgccctgcc ggaggcacag ctggaccgct
     6301 tcatgcttcg cctgcggctc ggctatccga aggcgatgga ggagatcgtc atcctcgacg
     6361 agcagaagcg aaagcacccc atcgaccagt tgcagcaggt cctcgacatc gacgagctgc
     6421 gcgagatgca ggcggcggtc aaggagatct acgtcgacca ggcggtggcc gagtacatcg
     6481 tgcggctgac cggcgcgacc cgggagcacg ctgacgtcta cctgggtgcc tcgccacgcg
     6541 gctccatcaa cctgtacagg gcgggccagg cactggcggc cctcgacggc cgcgactacg
     6601 tgatccccga cgacgtcaag cagctggccg tggcggtgct tgggcatcgg ctgatcgtga
     6661 agagccaggc gtcgctgcgc gaggtcgacc ccgatggcat cgtgcgcgag atcctgggcc
     6721 aggttccgat cgcagaggcc agcagcgcga ccgcaggcac gcgctaggca cggtcatggg
     6781 cctgtcgcgg cagctccggg ccgtcttctt cggcaccgcg ctggtcgtcg ccgcgttcag
     6841 caccgggatc gacgaactct tcttcctggt ctacctgctc gccaccctcc tgctcgcctc
     6901 gcgctggtac gcgcgccgcg gcctgcgcgg cctgcgtgcc ggctaccacg tgctcaaccc
     6961 gcggagccag gtgggcgaca cactcgaggc ggtgtatcgg gtcgacaacg acagcggctg
     7021 gacgaagccg tgggtcgagg ttgccaatga gtccaccctg cccgcgccgc tgccgggccg
     7081 cgtcgtgggc gtgaaggccc gcggcagccg gcagtggctg gccaaggtcg tcctgctgcg
     7141 tcgcggcagc taccggctcg gcgccttgcg ggttcggacc ggtgacccgt tcgggctctt
     7201 ctcgagcgag atggtggtgg gacggccgac ctggatcatg gtcttcccac gcgttcaccc
     7261 gttaccgcac tggcgtctgc ctccctcgcc ggtggacgga tcgacgccct cgcggcgacg
     7321 ctacgaggcg gcgacacctt tggtcagcac ggtgcgtcca tacgtgtatg gggacgcgat
     7381 caaccgcatc cattggctca gctccgtgcg ccacggcgag ctgatcgtga aggagttcga
     7441 ccttgagcag gcggccgacc tgtggctgct gctcgacctg cagcgcgccg cgcacgccgg
     7501 gccggccgag tcgtcctcgg tcgagaccgc ggtcagcgcg gccgcctcca tcgcggtcca
     7561 caccctggcc gagaaccggg ccgtggggat aacggtcagc tcgcgacgcc agcagcatct
     7621 caacccggat cgcggcacgc gggtggagca gaagatcctg aacctgcttg ccaacgtgca
     7681 ggccgatggc tcgcagccgc tcgccgaggt cgtggtctcg acactccccc agctgcggcg
     7741 cggcatgacc ctgtgcatcg tcaccggctc gaccgatcgc gaatgggtgc gcgccctggc
     7801 cgccctgcgg cggcggggga tcggcacgat cgtggtcctg ctcgatcgct tcagcttcgc
     7861 gggacgggac gacgacgaga gtggtgccga gctggcggcg atccggcacg cgctggccga
     7921 gtacgacatt gcgcatcatc tcctgcgagc cggagacgac ctctccagcg tccttgggcg
     7981 ccgggcgatc ggcgcgcgca ccccggccat tcgggagcgc acccgtgcct gagctgttgc
     8041 gtcgcttcct gcgaccgcgc gagggctggg gcagcatggc gctcctgatc gtcatgctcc
     8101 tcacgctggt gtggtccctg cagcgcgcgg cgtgggtcag tcacatggac tacctgctcc
     8161 ctgtcgcggt ggtggccgcg ctcctcgggg cgctgctggg gctgacccgg ctgagcgtga
     8221 cggtcatcct gccactcgcc gcgatcgcag gagccggcct ggtgctgttc acgatcggcg
     8281 ccgagtactt cccggagctg aaccagagca gtcggctgat cctcctgcgc ggtgatgcgc
     8341 tggattggac tcggatcgtg gtcgagggcg ggttcgcccc ccagctctct ccttatgcaa
     8401 tcggactcgg cgtgctgatg tgggtcacct ccttcatggc cgcgtacgcg ctgtaccgcc
     8461 accatcgggt cctcgacagc atcctgctgg tgggcgtggc cctcatcgcg aacatgtccg
     8521 cgatcctcac ggacctcttc ctgtacctcg tcctcttctc gctggccgcg ctgctgctct
     8581 gggtgcggat cgccctcgtc aatcgcgagg agagctggcg catccgccgg ctacgggaga
     8641 acctcgacgt gccgggtcag ataatgcgca gcggcgtcgc cttcatcgtc ggcagtgtcg
     8701 ccctggcctg ggtgctgacc acggtcgccg tggctgcacc cctgaccagc gtctggagca
     8761 acctcgatgg cctgtggtcg gatgtgcgca accagttcga gggggccttc ggcggcctcg
     8821 ccggcaccga ttcccgcatc caggggacat ccttcggctc ttcgttccga gtgcacggca
     8881 gctggtccag ctccgatggg gcggtcatga cagtgggctc gacccgcggt tactacatgc
     8941 gcaccatcac ctacgacacc tacaccgggc acggctggtc gaccagcgac ggcccgaacc
     9001 ggcgcgtcgc agccggcgat cgcatcttcc ccggctacac ccctgagcgt ccgctggcgc
     9061 ccagcgcctt ccagatcgag acagtgactg tcgaggtgca gcgcaacgcc gggcgcaatg
     9121 tcttcacacc cggctatccg acgaccgcat tcgccccgct ggttgtccac gagccacgcg
     9181 gcctgccact gctcggcgca ttgcaatccg cggtggttct ccaggagggg aagggctacc
     9241 agatcaccgc ggcgatctcg actgccaccg aggcgatgct ggcgggcgcc ggcacggatt
     9301 atccgcccga gatcgtggcg acctacaccg gtaccggcgg tgtcacccag cggaccgccg
     9361 acgaagcgcg ccgcgtggtc aacgccgccg gcgccacgga cccgtaccac gaggccaggg
     9421 cactggccga ctacctgcgc accgaccgct tcacctacca gaccgtggcc tctctcccgt
     9481 ccgacccgaa ccgcgacctc gtcgacttct tcctgttcga ccccgcgggg caggtcggct
     9541 actgcgagta cttcgccacg accatggcgg tcatggcgcg gacgctggga ctgccggcgc
     9601 gcgtggccgt cggctacgcc cccggagagc ggctccaacc ggggatctac cagtaccgcg
     9661 aacgcaatgc gcacgcctgg gcggagatct tcttcccggg ctatggctgg cagatcttcg
     9721 aggccaccaa gtcgattgct cccgtgatcc gcgcgcccgg ggcgggggtc gtgccccctg
     9781 ttcccccggc caacggccag atcgacccgg gtcgccgcgt ccccgaggac cagaagactg
     9841 gcaccatctc cggcctgccg tccttcgagc cggtcgaggg cggcttcgcc cccggaggcc
     9901 agcgcccgac cgacgaggcc cgcaccggca acgcctggtt gatcgtcgtg ctgctgatgc
     9961 tgctgctggc ggtcgtcgcg tggcggctgc tgcgcggccg gtatcggttc aggttcctcg
    10021 ccccgggcga tcggcaatgg cagcgcctcg cctttgccgc ggatcgggcg ggggtcgcgc
    10081 agcgaccatc ggagaccgtg tacgagtacg ccggctggct tgaggggcag ctcccggggc
    10141 gccgcgtcga gatccacgac atcgccgacg gcaaggtgtg gcagagctac tcaggccggt
    10201 ccatgtcgtc ggaggtgatc gcgcgcctcg agcgtgcctg gggccggctg cagctgccgc
    10261 tcctgtggct cgcgctgcgc aggcgccttc ggtccttgct ggggagcggg agctagcctc
    10321 agccgaccgg ggcggcctgc ctcgcctcga agacttctcg catgacgcgg accgccgccg
    10381 gcgcatcggc ctggcggccg tgctcggcaa gcgtctcgcc catgatgcgg accgcgtccg
    10441 tcatctcgtc gatgccgacc ccgcccatgt gcccgaatcg caggatccgt cctgcccatt
    10501 gcccctggcc gcccgccacg atcagccccc tggcgcgcat ggcggcgttg aagggcgccc
    10561 attcgaggcc ttccggcagc catgcggcag tgaccgtgct ggaccgaacc gcaggggcag
    10621 ccgccaggcg cagtccaagg gcctcgagcc ccgcctgcac ggcggcggcg atggcctcgt
    10681 gcctggccca ggtccgctct cggccctccg cgacgaggcg gcggacgccg acccgcaggc
    10741 cgaacaggac gctgaccgcc ggggtccagg gagtctgacc cttctcggcc cacatgcggg
    10801 cctccgcgag gtcccagtag gcgcgcggca tgcgcgcggt ctcgttggcg ctccatgccc
    10861 ggtctccgag cgcggcgatg gcgatccccg gcgaccccat ccaggccttc tggctggcgc
    10921 tcaccaccag gtcgatcccc cagccgtcca tctcgaacgg catcgcgccc agcccgctga
    10981 tcccgtcgac gagcaccagc ggatctcccg gcgccttgcg gacggcctcg gccagctcgc
    11041 ggagggggtt ggtcacgccc gtcgacgtct cgttgtgcgt gagcaggacg gcccggtacg
    11101 ctggattcgc cgccaggtgc tgcgccaggg ctcccgggtc agccgcctcg ccccacgcga
    11161 cttcgaagcg ctcgacccct gccccgaacg cagtggcgat cttggcaaag cggtcgccaa
    11221 atgagccgat ggtcacggcg agcacccggt cgcccggcga gagggtgttg acgacggctg
    11281 cctccagcgc ccctgagcca gagccggtca gcagcagcac ctccccgcgc gtgccgatca
    11341 gctcggcgag ccctgtgctg atctcacgga gcagctctcc gaattcggtg ccgcgatggt
    11401 cgatcatcgg cgccgattgg gcctcgagcg cctccggcgg gagcggcgtg gggccaggga
    11461 cgcgcaggtt ctgttccatg gccggcatca taatcggccc atggaaggga ccgatgcccc
    11521 gtagctctcc gccccgcagg cca
//
LOCUS       DATCQR010000140         2653 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_178469, whole genome shotgun sequence.
ACCESSION   DATCQR010000140 DATCQR010000000
VERSION     DATCQR010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2653
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178469"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..958
                     /locus_tag="VIN32_04760"
     CDS             <1..958
                     /locus_tag="VIN32_04760"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEY8921923.1"
                     /translation="PLPLFGLVFYLGAIWLTIRALDTRSIAGLSPEAALVGLAIAGAV
                     ASAVLTALELFVIRAFCSWCLVQAAASFALLVAAILLMHTPTEPVVAHSRRTRQQAAR
                     AREGERSSLLRTGRAGGAVATLAVVALLALGAGGETPTGSPGPSGIAPAGSPRLGSGA
                     IEVVEFADFQCPGCAVLAPIVADLASANEMTLVSRYFPLDAIHPNADRSARAAAAADR
                     QGSFWQMSAALYARQDSWKDLSGNDADAFFTSLAGELGLDTAQWQASYASSEIVAVVD
                     VDRQAAITMGLNSTPTLFIGGTHYTGPLTADGIRAAIAAVSP"
     gene            1035..>2653
                     /locus_tag="VIN32_04765"
     CDS             1035..>2653
                     /locus_tag="VIN32_04765"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEY8921924.1"
                     /translation="MGTAVSRPTDASPLPRYGRGGLSSRVRHLIRPRARRYLFRDPFF
                     LYTAALAPIALTVALISHEPRDVPMVAILSPIFIGLQMVLSLLPTRLRPLTKLGWSFL
                     RLAVSLLYVAALAELVGGPTHPMFALYVPVVVAAAALGTSQAVVIGAAASLIYLGPEL
                     AIPGSRAELALRGISLAGVSILVAIGTRRLVMAVEQTTGQLRAAMVAERRHSRQIAGM
                     EAVSHLLASAGPTPEMLDRALGVLVDQFHYTYVSIYLAEGDLLCLGAQRGYQQPRESF
                     DGTEGVVGRVMRTHEPAFIQDVRLDDDYVAVDDDVVSEICAPLLYDAEFLGILNVEAL
                     APLDRTDRDLIATLAGRVATEVALGRDRQTLAERAAVLRSLSDFTNAVSGELDLARLG
                     AVMVDAVRKVIPADIVTLTVLERETGRYLLRAVTDVDPSILGREIKPGEGLAGRAIRD
                     RTVVVDDHFDRDRFPAAYRDSAEPMPMLGAGVPLLRDGVVVGAISIMRRDRSNRFRPM
                     EHEAMDLLAGHAGLAISNAFLHAEVEQLATH"
BASE COUNT          363 a          915 c          921 g          454 t
ORIGIN      
        1 cccgctgccg ctcttcgggc tggtcttcta cctcggggcg atctggctca ccatccgcgc
       61 cctggacacg cgctcgatcg cgggactgtc gcccgaggca gcactggtgg gccttgcgat
      121 cgccggggcc gttgcctcgg cggttctcac ggcgctggag ttgttcgtga tccgggcatt
      181 ctgcagctgg tgcctggtgc aggccgcggc cagtttcgca ctcctggtgg ccgcgatctt
      241 gctcatgcac accccgactg aaccggtggt ggcacattcc aggcggacgc gccaacaggc
      301 ggcgagggca cgtgaaggcg agcggtcgag cctgcttcgc accgggcgcg cgggtggcgc
      361 agtcgcaacg ctcgccgtgg tcgcgctcct ggcgctgggt gccggcggcg agacgccgac
      421 agggtcaccg ggtccatcgg ggatcgcgcc ggcgggaagc ccccgcctgg ggagtggcgc
      481 gatcgaggtg gtcgagttcg ccgacttcca gtgccccggc tgtgccgtcc tggcgccaat
      541 cgttgcggac ctcgcctcgg cgaacgagat gacgctcgtg tctcgctact tcccgctcga
      601 tgcgatccat cccaatgcgg atcggtccgc ccgagccgcc gccgccgcag atcggcaggg
      661 atcgttctgg cagatgtcgg cggcgctgta cgcccgccaa gactcctgga aggacctgtc
      721 cgggaacgat gccgacgcct tcttcacgag cctcgccggc gagctggggc tcgacaccgc
      781 ccaatggcag gcgtcatacg catcgtctga gatcgtcgcg gtcgtcgatg tcgaccgcca
      841 ggcggcgatc accatggggc tcaactccac tcccaccctg ttcatcggcg gaacgcacta
      901 cacggggccg ctcaccgcgg acggcatccg ggccgcgatc gccgccgtaa gcccctgatc
      961 gaggccgttt cactagtacg ggttacttcc gggcgatggt ggggtctggt ctggatggct
     1021 agggtctgct cccgatggga accgccgtat cgcggccgac cgacgcgagt ccattgccgc
     1081 gctatgggcg aggcggcctg agctcgcgcg tccgccatct cattcgtccg cgggcgcgcc
     1141 gttacctctt cagggacccg ttcttcctct ataccgccgc cctggcgccc atcgcgctga
     1201 ccgtcgccct catctcacac gagcctcgcg acgtgccgat ggtcgccatc ctgagcccga
     1261 tcttcatcgg cctccagatg gtcctgagcc tccttccgac caggctccgg cccttgacga
     1321 agctcgggtg gtccttcctg cgcctcgccg tcagccttct ctacgtcgcc gccctggccg
     1381 agctggtggg cgggccaacc caccccatgt tcgcgctgta cgtgccggtg gttgtcgccg
     1441 ccgcggcgct ggggacgagc caggcggtgg tcatcggcgc agcagcgtcg ctcatctacc
     1501 ttggccccga gctcgcgatt cctggctccc gtgccgagct cgcattgcgc gggatcagcc
     1561 tggcgggggt cagcatcctg gtggcaatcg ggacgcgtcg gctggtgatg gcggtcgagc
     1621 agacgacagg ccagctgcgt gcggcgatgg tcgccgagcg gcggcactct cgccagatcg
     1681 caggcatgga ggcggtcagc catcttctgg cgtcagcggg tccaacgccc gagatgctgg
     1741 atcgtgccct gggcgtgctg gtcgaccagt ttcattacac ctacgtctcc atctacctcg
     1801 ccgaaggcga cctgctctgc ctcggggcgc agcgcggcta ccagcagccg cgagagtcat
     1861 ttgacgggac ggagggggtg gtggggcggg tgatgcgcac ccacgagccg gccttcatcc
     1921 aggacgtgag gctcgacgac gactacgtgg cggtcgacga cgacgtggtg agcgagatct
     1981 gcgcgccgct gctctatgac gccgagttcc tggggatcct caacgtcgag gcgctcgcgc
     2041 ccctggaccg caccgatcgt gacctgatcg cgaccctggc cgggcgagtg gcgacggagg
     2101 tggccctcgg gcgcgaccgt cagacgctcg ccgaacgggc agcggtgctg cgcagcctga
     2161 gcgacttcac caacgcggtg agcggagagc tcgacctggc acggctcggt gccgtgatgg
     2221 tcgacgcggt gcgcaaggtg atccccgcgg acatcgtgac cctcaccgtc ctggagcgcg
     2281 agacggggcg ctacctgctg cgcgcggtga ccgacgtcga cccgtcgatc cttggccgtg
     2341 aaatcaagcc aggcgagggc ctggccggac gcgccatccg agatcggacg gtggtggtcg
     2401 acgaccactt tgaccgcgat cgattccctg ccgcctaccg agactccgcg gagccgatgc
     2461 cgatgttggg cgccggggtg ccgctgctgc gggatggcgt ggtggtgggc gcgatctcga
     2521 tcatgcgccg cgatcggtcg aaccgcttcc ggccgatgga gcacgaggcg atggatctgc
     2581 tggcaggcca tgccgggctg gccatctcga acgcgttcct ccacgccgag gtcgagcagc
     2641 tcgcaacgca cga
//
LOCUS       DATCQR010000141         1748 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_224081, whole genome shotgun sequence.
ACCESSION   DATCQR010000141 DATCQR010000000
VERSION     DATCQR010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1748
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224081"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(50..886)
                     /gene="atpB"
                     /locus_tag="VIN32_04770"
     CDS             complement(50..886)
                     /gene="atpB"
                     /locus_tag="VIN32_04770"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEY8921925.1"
                     /translation="MVHVEIAAEKLFAIGPLIVTNSMVGAVLASVVLLLIARWFTRRS
                     GIVPSRGQSAIELPIEFLAGVVRGTGGKRWRQFAPLILGIFLFVLTANWLSLLPGVGT
                     IGLGEEVGGKQIIVPFVRAGAADLNFTLGLALISFVTFVAFGLRANGARGYLKELLIA
                     EPAYMTPLLTPIHLISELSRVISLSMRLFGNVFAGEVLLATMLALTTATYFTLPLAFV
                     VPGVFIGLELLFGLVQALVFALLSMTYITLAIAEHRQHASADDHSGEAPEQGAAPAPG
                     AA"
     gene            complement(894..1139)
                     /locus_tag="VIN32_04775"
     CDS             complement(894..1139)
                     /locus_tag="VIN32_04775"
                     /inference="COORDINATES: protein motif:HMM:NF021073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HEY8921926.1"
                     /translation="MRAGAMGPDQGKDEGPPTANWAVAFDLGLRLGISVILGVGGGLL
                     VDSWLHTSPIFTLIGMVLGIGAAMYTIWDVARQSMRR"
     gene            complement(1114..1338)
                     /locus_tag="VIN32_04780"
     CDS             complement(1114..1338)
                     /locus_tag="VIN32_04780"
                     /inference="COORDINATES: protein motif:HMM:NF021073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HEY8921927.1"
                     /translation="MAQLTPTGLVKIMGLVTLIMVIPIVGGSVAGLVLDRVFGTSPLL
                     VVIGLAVGSLIAALGIWLLIRAGARRGYGA"
     gene            complement(1409..>1748)
                     /locus_tag="VIN32_04785"
     CDS             complement(1409..>1748)
                     /locus_tag="VIN32_04785"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897903.1"
                     /note="catalyzes the reaction of glycine with
                     5,10-methylenetetrahydrofolate to form L-serine and
                     tetrahydrofolate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEY8921928.1"
                     /translation="LMLVDVRPLGVTGKEADLALGEVRITVNRNTIPYDPNPPMVASG
                     VRVGTPAVTGRGMKEAEMGQIAQLIVDGIAARGDDGAQAAIRDRVAEIVDRFPVPGLP
                     QTLVAGQTTA"
BASE COUNT          307 a          609 c          580 g          252 t
ORIGIN      
        1 ggctgcgagt gtgtgtaggt ccacgagtgg tcttcctcct tgtggttgct tacgccgcgc
       61 cgggtgccgg agcggcgcct tgctcgggcg cctcgcccga atggtcgtca gctgaggcgt
      121 gctggcgatg ctcagcgatc gccagcgtga tgtaggtcat cgagaggagc gcgaagacga
      181 gcgcctgcac cagcccgaag aggagctcga ggccgatgaa gacccccggc acgacgaaag
      241 caagcggcag cgtgaagtac gttgccgtgg tgagcgccag catggtcgcc agcagcacct
      301 ctccggcgaa gacgttgccg aagagccgca tcgagaggct gatcacccga gacagctcgc
      361 tgatcaggtg gatcggggtg aggagcgggg tcatgtaggc gggctcggcg atcagcagct
      421 ccttcaggta cccgcgcgcg ccgttggcac gcagcccgaa ggcgacgaag gtcacgaagg
      481 agatcagcgc caggcccagc gtgaagttca ggtcggcggc tccggcgcgc acgaacggga
      541 cgatgatctg ctttccaccg acctcctcgc caagcccgat cgtcccgacc ccgggcagca
      601 ggctcagcca gttggcggtc agcacgaaca ggaagatgcc caggatgagc ggcgcgaact
      661 ggcgccagcg cttcccaccc gtgccccgca ccacgcccgc gaggaactcg atcggcagct
      721 cgatcgcgct ctgcccgcgg ctcggcacga tcccggagcg gcgcgtgaac cagcgggcaa
      781 tgagcagcag gacgaccgag gcaaggaccg ccccgaccat cgagttggtg acgatcagcg
      841 gcccgatcgc gaacagcttc tcggccgcga tctcgacgtg taccactccg cgcctatctc
      901 ctcatgctct gccgcgccac atcccagatc gtatacatgg cggctccgat ccccagcacc
      961 atcccgatca gcgtgaagat cgggctcgta tgcagccagc tgtccaccag cagcccgccc
     1021 ccgactccca ggatcaccga gatccccaac cgcaggccca ggtcgaaggc caccgcccag
     1081 ttggcggttg gcggaccttc gtccttgccc tggtcaggcc ccatagcccc ggcgcgcacc
     1141 agcgcggatg aggagccaga tgccgagtgc cgcaatcagg cttccgaccg ccagaccgat
     1201 gacgaccagc agcggcgacg tgccgaatac ccggtccagg acgagcccgg cgaccgagcc
     1261 tccgacgatt gggataacca tgatcagggt caccaggccc atgatcttga cgagcccggt
     1321 cggggtcagc tgtgccatct tcagttcgac tcagagcggt cggacggcgc gagtataccg
     1381 gagcccgatc gacccggcga cgcggcgcct aggcggtcgt ctgtcccgcg acgagcgtct
     1441 gcggcagtcc ggggaccggg aatcgatcga cgatctcggc cacgcggtcg cggatggcgg
     1501 cctgcgcccc gtcatcaccc cgggccgcga taccgtccac gatgagctgg gcgatctggc
     1561 ccatctccgc ctccttcatc ccgcggccgg tcaccgcggg cgtgccgacc cggaccccgc
     1621 tcgccaccat cggcgggttg gggtcgtagg ggatcgtgtt tcgattgacg gtgatgcgga
     1681 cctcgccgag cgccaggtca gcctccttgc ccgtcacgcc gaggggtcgc acgtcgacga
     1741 gcatcagg
//
LOCUS       DATCQR010000142         2855 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_147667, whole genome shotgun sequence.
ACCESSION   DATCQR010000142 DATCQR010000000
VERSION     DATCQR010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2855
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147667"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            382..1623
                     /locus_tag="VIN32_04790"
     CDS             382..1623
                     /locus_tag="VIN32_04790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921929.1"
                     /translation="MSRLARRVGALSVLLLSLAIVAVVGLRATAVGLLPSPTPVAATA
                     TPRPSDPVPSQDLVSAFTEIEAQVQQLRGLPAAEIGPPQILTRAELEQVLPALLEPAL
                     DNATLRALGLLTAEQDIVKLTERLYVAQVAGYYDFDAKRMVVVTDAGLTPAAQITYAH
                     EYTHALQDAAFDSSAAQQAVAGQRDRELALLALEEGDATTSMVVWAIGHLSPDEVAGV
                     AQAPVPDMSGIPPWMVRLLEFPYLSGAEFVGQLYASGGWDAVNAAYDKLPASTEQILH
                     PAAYANGEAAVAVAPLDLRSALGADWGQASDTTLGEAWMAIWLEGIGVTRPVADEAAK
                     GWGGDRVTVATNGDGGWALGWRIAWDTSADATEFETAYATAQPKLPFAARLVHVSDLE
                     TLVLQASSADLIETIAILAGG"
     gene            complement(1626..>2855)
                     /gene="argS"
                     /locus_tag="VIN32_04795"
     CDS             complement(1626..>2855)
                     /gene="argS"
                     /locus_tag="VIN32_04795"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEY8921930.1"
                     /translation="RGAFVGDLLCRVLEGVGHEVTREYYFNDFNAQVLNLGLSVQARR
                     EDGPIPEDGYHGEYVAELAAAIPDETWADATAEGSDAGSVLGRWASERVRAGIEESLT
                     RLGVRFDVWTSEGSIHEQGWVTRAVEKLRDAGHIYEADGATWFRSTAFGDDKDRVVIR
                     SNGQPTYFASDLGYIAQKFSRGFGELIYLWGEDHHGTVARNRAAARALGFDAEAVHWM
                     LMAWVRFVRDGVEIGMSKRSGEFISLDELLEEIGPDAARWYFGSRATTTGIDLDIELA
                     KKQSAENPVYYVQYAHARCSSILRRAADEKLAPDASDAALLLVHPAEQALLRRLLAMP
                     DVVADAAERRETHELTHFCLEVAQLFSAFYRDCRVLPEEPSEIPLSQARLALTAAARQ
                     VLANALGLLGISAPDSM"
BASE COUNT          450 a          972 c          989 g          444 t
ORIGIN      
        1 gccagcggtg atcgagcggg tgtagctgtg gtcggcatgg gcgtgcgaga tcgtcagcag
       61 gtcgacgtcg tgcttgcccg ccacgaaccc ggttgcgggc gggcacgggt cggtgatgac
      121 cgtcgcctcg cgccccttca gtcggaagca ggcacgcccg taccaggtga tctccatggg
      181 atggccgatg ctactggaaa cgcacgagcg ccggtgcccg cccaatgaca ccggcgctcg
      241 aaggagggag ggtggaggcg gaaacccgtt ggtttcctac tcgcagggac accctaaaag
      301 ccgacggtga atcccaaagg ggggcttggc ttaagaaacc tgaacctaac gccggatcag
      361 gcgggtgcca caatgcggcc gatgtcccgt cttgccaggc gcgtcggcgc cctcagcgtg
      421 ctcctgctga gcctggccat cgttgcagtc gtcgggcttc gcgccacagc agtcggcctg
      481 ctccccagcc caaccccggt tgcggcaacg gccactcccc ggccatcgga cccggtcccc
      541 tcacaggatc tggtgtccgc cttcaccgag atcgaggcgc aggtccagca gctgcgcggc
      601 ctgccggcgg ctgagatcgg tccaccgcag atcctgaccc gggccgagct ggagcaggtg
      661 ctgcccgccc ttctggagcc ggccctcgac aacgccaccc tgcgcgccct gggcctgctt
      721 accgccgagc aggacatcgt gaagctgacc gagaggctgt acgtcgccca ggtggccggc
      781 tactacgact tcgacgccaa gcggatggtg gtcgtgaccg atgcagggct gacgcccgcg
      841 gcgcagatca cctacgccca cgaatacacg catgccctgc aggacgccgc cttcgacagc
      901 agtgcagccc agcaggcggt cgccgggcag cgagatcgtg agcttgccct gctcgcgctc
      961 gaggaggggg atgcgaccac gtcgatggtg gtgtgggcga tcggccacct ctcgcccgac
     1021 gaggtggccg gggtggcgca ggcccccgtt cccgacatga gcggcatccc gccatggatg
     1081 gtccggctgc tcgagttccc ctacctctcg ggcgccgagt tcgtgggcca gctgtacgcg
     1141 tcgggcgggt gggatgccgt gaacgccgcg tacgacaagc tgcccgcctc cacggagcag
     1201 atcctgcatc ccgcggcata cgccaatggc gaggctgcgg tcgcggttgc acccctcgac
     1261 ctccgaagtg cgctcggagc cgattgggga caagcctccg acacgaccct cggcgaggca
     1321 tggatggcga tctggctgga ggggatcggc gtcacgcggc ccgttgcgga cgaggccgcg
     1381 aaggggtggg gtggcgaccg ggtgacggtg gccacaaacg gggacggtgg ctgggcactt
     1441 ggttggcgga tcgcctggga tacgtccgcc gatgcgaccg agttcgagac cgcctacgcg
     1501 acggcccagc ccaagctccc gtttgcggcg cggctggttc atgtctcaga tctggagacg
     1561 ctcgtcctgc aggcctcgtc ggccgatctg atcgagacga tcgccatcct ggcggggggc
     1621 tgagactaca tcgagtccgg ggcagagatg cccagcaggc cgagcgcgtt ggccagcacc
     1681 tggcgggcag cggccgtgag cgcaaggcgg gcctgcgaga gcggtatctc cgagggctcc
     1741 tcgggcagga cgcggcaatc gcggtagaac gcgctgaaga gctgcgcaac ctccaggcag
     1801 aagtgggtca gctcatgggt ctcccgtcgc tcggcggcat cggccacgac gtccggcatg
     1861 gccagcaggc gtcgaagcag cgcctgctcg gcgggatgca cgagcagcag agccgcgtcg
     1921 ctggcatcgg gcgccagctt ctcgtccgcg gctcgacgca ggatcgagct gcagcgggcg
     1981 tgggcgtact ggacgtagta gacggggttc tccgcgctct gcttcttggc cagctcgatg
     2041 tcgaggtcga ttccggtcgt cgtcgcccgc gagccgaagt accagcgcgc cgcatccggc
     2101 ccgatctcct ccagcagctc gtcgaggctt atgaactcgc cggagcgctt gctcatgccg
     2161 atctcgacgc cgtcccgcac gaagcgtacc cacgccatca gcatccagtg cacggcctcg
     2221 gcgtcgaacc ccagcgcccg ggcggcggcg cggttacggg ccacggtccc gtggtggtcc
     2281 tctccccaca gatagatcag ctccccgaag ccgcggctga acttctgcgc gatgtacccc
     2341 aggtcggagg cgaaataggt gggctggccg ttggagcgga tcaccacccg atccttgtcg
     2401 tcaccgaacg ccgtggagcg gaaccaggtc gccccatcgg cctcatagat gtggccggcg
     2461 tcgcggagct tctcgactgc gcgggtgacc cagccctgct cgtggatcga tccttcgctg
     2521 gtccacacgt cgaagcggac gccgagccgg gtgaggctct cctcgatccc ggcgcgaacg
     2581 cgttccgagg cccaacgacc caggaccgat cccgcgtcac tgccctcggc tgttgcgtct
     2641 gcccatgtct cgtcgggaat cgcggcggcg agctcggcaa cgtattcgcc gtggtagcca
     2701 tcctcgggga tcggcccgtc ttcgcggcga gcctgcaccg agaggccgag gttcagcacc
     2761 tgggcgttga agtcgttgaa gtagtactcg cgcgtcacct cgtggccgac cccctccagg
     2821 acgcgacaca gcaggtcgcc gacaaaggcg ccacg
//
LOCUS       DATCQR010000143         2733 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_54522, whole genome shotgun sequence.
ACCESSION   DATCQR010000143 DATCQR010000000
VERSION     DATCQR010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2733
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54522"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..493)
                     /locus_tag="VIN32_04800"
     CDS             complement(<1..493)
                     /locus_tag="VIN32_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HEY8921931.1"
                     /translation="MSNNLAYTDAYAATTPARVLQADDGAVLLDITVFYPGGGGQPAD
                     MGWLRTDEGAAWRVTGARKSGDDIWHLVEGDGSLPAVDTEVSVELDWDRRHALMRTHS
                     ALHVLCGVVWRDWGASVTGGNMEPLAGRMDFEFETMAGELVAEIERRVNVEIEADREI
                     RVAV"
     gene            531..2264
                     /locus_tag="VIN32_04805"
     CDS             531..2264
                     /locus_tag="VIN32_04805"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEY8921932.1"
                     /translation="MDFSATVDSLLEEYFRLQPADATEFGEHAYDHHWPDLTDAGQVA
                     WSDWLRDAEARVEALDPGALTRDEAIDRRILLENLAAMRFAADELREGEWNPISYVYL
                     FGAAIFTMLAREYAPITERLNALASRLRGLPVALEGAREALSREGGRPVSRFHAEKAV
                     ERMAGVADLAALAVSEAQALDEPALLADVQVAVGVATAAIEEWTKWLRDELLPTVDGD
                     FRLGPELYAAKFRHWLKSAVTPTELEKMAVEEYDQTRAEMTRIARAIWGDWMGDAPPP
                     ESDDEAVRSVLDAIAVDHPRADELLDFCRAENERIEAFVAEHDVVGLTEEPLQIIWTP
                     GFLRNFGGAMLIPPGPLDRGLASFFAITPVNEAWPAERRESYLREDNARMLRLLTIHE
                     AVPGHYLQLAYSNRCPSLVRGIFRSGVFAEGWAVYVTQVMMDLGYGADDPALMLVHWK
                     FYLRSATNALMDIRIQSGSMDEDEAMHMMVEGGFQEDGEATAKWDRARLSSTQLCEYF
                     LGSVEMHGLEREARRRAKAEGREFVYRPFLESVLAHGSPSLPVIRDILFSSDEGSPPA
                     KGSKESPAPST"
     gene            complement(2200..>2733)
                     /locus_tag="VIN32_04810"
     CDS             complement(2200..>2733)
                     /locus_tag="VIN32_04810"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEY8921933.1"
                     /translation="TSWAYSNTNYLIAGMLVERVTGHPFADELERRITGPLKLRGTGP
                     PTPGELPDLIGVSWTSAFFTSAMLDSNAANLVRWGDALYGGSILRADSLAQMLDFNDQ
                     GYGLGAERYRFGGLLGYGHSGLLRGYTALLAHLPDAHLTVSMLATGHLFSVSDLLTYS
                     GPGTPSILSLAGNLRPS"
BASE COUNT          426 a          947 c          942 g          418 t
ORIGIN      
        1 ggaccgcgac gcggatctcg cggtcggcct cgatctccac gttgacccgc cgctcgatct
       61 cggcgaccag ctcgccggcc atcgtctcga actcgaagtc catgcgtccg gctagcggct
      121 ccatgttgcc gccggtgacc gacgcgcccc agtcgcgcca gacgacgccg cacaggacgt
      181 gcagcgcgga atgggtccgc atcagggcgt ggcgccggtc ccagtccagc tcgacgctga
      241 cctcggtgtc aaccgccggc agactgccgt ccccctccac caggtgccag atgtcatcgc
      301 cggacttccg cgccccggtg acacgccacg cggcgccttc atcggtccgc agccagccca
      361 tgtccgccgg ctggccaccg cctcccggat agaagacagt gatgtcgagg agcacggctc
      421 cgtcatcggc ctggagcacg cgggcggggg tggttgccgc gtaggcatcg gtgtaagcga
      481 ggttgttgct cacgccgagc aggctagcag gcggtaccat gccgtgcgcc atggacttct
      541 ctgccaccgt cgacagcctg ctcgaggaat acttccgcct ccagccggcc gatgccaccg
      601 agttcggtga gcatgcctac gaccaccact ggccggacct gaccgatgcg ggccaggtgg
      661 cttggtccga ctggctgcgc gatgccgagg cgcgcgtcga ggctctggac ccgggtgcgc
      721 tgacgcggga tgaggcgatc gaccggcgga tcctgctcga gaacctggcg gccatgcgct
      781 tcgcggccga cgagctgcgc gagggcgagt ggaaccccat ctcctacgtc tacctcttcg
      841 gcgccgcgat cttcacgatg ctggcgcgcg agtacgcgcc gatcaccgag cgactgaacg
      901 cattggcgtc ccgcctgcga ggcctgcccg tcgcactcga aggggctcgc gaggcgctga
      961 gtcgcgaggg cggccggccg gtgagccgct tccacgccga gaaggccgtg gagcgcatgg
     1021 caggcgtcgc cgacttggcc gcgctggccg tgtcggaggc ccaggcgctt gacgagcccg
     1081 ccctgctggc cgacgtgcag gttgctgtcg gtgtcgcgac cgcggccatc gaggagtgga
     1141 cgaagtggct tcgtgacgag ctgctgccga ccgtcgacgg cgactttcgc ctcgggccgg
     1201 agctgtacgc cgccaagttc cgccactggc tgaagagcgc ggtcacgccc accgagctcg
     1261 agaagatggc cgtcgaggag tacgaccaga cccgcgccga gatgacccgc atcgccagag
     1321 ccatatgggg cgactggatg ggcgacgccc cgcctccgga gagcgacgac gaggcggtgc
     1381 gcagcgtcct cgacgcgatc gcggtggacc acccgcgcgc ggacgagctg cttgacttct
     1441 gccgtgcaga gaacgagcgg atcgaggcat tcgtggccga gcacgacgtc gtcggcctga
     1501 ccgaagagcc gctccagatc atctggaccc ccggcttcct gcgcaatttc ggcggcgcca
     1561 tgctgatccc gcccggtccc ctggatcgcg gcctggccag cttcttcgcg atcacccccg
     1621 tcaacgaggc gtggccggcc gagcggcgcg agtcgtacct gcgcgaggac aacgcccgga
     1681 tgctgcggct gctcaccatc cacgaggcgg tccccggcca ctacctgcag ctggcctatt
     1741 cgaaccgatg tccgtcgctg gttcgcggca tcttccgctc cggggtcttc gccgagggat
     1801 gggcggtgta cgtcacccag gtgatgatgg acctgggcta cggcgccgat gatcccgcgc
     1861 tgatgctcgt ccactggaag ttctacctgc gctctgccac caacgcgctg atggacatcc
     1921 gcatccagtc cggctcgatg gacgaggacg aggcgatgca catgatggtc gagggcgggt
     1981 tccaggagga cggcgaggcg accgccaagt gggaccgcgc gcggctctcc tcgacccagc
     2041 tgtgcgagta cttcctgggg agcgtggaga tgcacgggct ggagcgggag gcacgccgcc
     2101 gggccaaggc ggagggtcgc gagttcgtct accggccgtt cctcgagtcg gtcctggccc
     2161 acggctcgcc gtcgctgccc gtgatccgcg acatcctgtt cagctcggac gaaggttccc
     2221 cgccagcgaa aggatcgaag gagtccccgg ccccgagtac gtgagcaggt ccgacacgct
     2281 gaagagatgc ccggtggcca gcatcgacac cgtcaaatgg gcgtccggga ggtgggccag
     2341 cagggcggtg tagcctcgca gcagccccga atgcccgtag ccgagcaggc ccccgaaccg
     2401 ataccgctcc gcacccagcc cgtatccctg gtcgttgaag tcgagcatct gcgccaggct
     2461 gtccgctcgc aggatggacc cgccgtacag cgcatcaccc cagcgcacca ggttggccgc
     2521 attcgagtcg agcatggctg atgtgaagaa cgcgctcgtc caggagacgc caatcaggtc
     2581 gggcagctcg cccggcgtcg gggggcccgt accgcgtagc ttcagcggcc cggtgatgcg
     2641 acgctccagc tcgtcggcga aggggtggcc ggtgacgcgc tcgaccagca tcccggcgat
     2701 caggtagttc gtgttggaat aggcccagct cgt
//
LOCUS       DATCQR010000144         5245 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_39092, whole genome shotgun sequence.
ACCESSION   DATCQR010000144 DATCQR010000000
VERSION     DATCQR010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5245
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39092"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(107..532)
                     /locus_tag="VIN32_04815"
     CDS             complement(107..532)
                     /locus_tag="VIN32_04815"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEY8921934.1"
                     /translation="MTTAANDLRVGDLMSNDPVVIGADAQATEAERLLKTYRVSGLPV
                     VSGNATVGVISQTDLAMARSSAMISGNWARLQVRHLMTTPAVTVRTGTSLKRAAQLMV
                     TRHIHRLVVVDDEEVPIGVISSLDLLRTLLEDPDLRPGA"
     gene            complement(599..1000)
                     /locus_tag="VIN32_04820"
     CDS             complement(599..1000)
                     /locus_tag="VIN32_04820"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEY8921935.1"
                     /translation="MKKILVAYDGTEPAEHALATAIDLAKAFDAAVGVVSVVPIHAGR
                     APIDPWDDRPIHAAQLGQAQQILAKAGIEAQLHEPMGDPARAIERTAAESGYDTIVIG
                     SRGLGRLERVLQGSVSEHVATHATETVIIRR"
     gene            1304..2359
                     /locus_tag="VIN32_04825"
     CDS             1304..2359
                     /locus_tag="VIN32_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEY8921936.1"
                     /translation="MRAAVFHAPGDIRVEKVARPRPGPGEAVIRVTLTTICGTDVHIL
                     KGEYRVEPGLIVGHEPVGIIEELGPGVVGYNVGERVLVGAITPCGQCHACLSGKLSQC
                     GHGTGYEAIGGWRFGNTINGAQAESLLVPSAQANLAKIPDTLTDEQVVLLADIASTGF
                     AGAESGKVRIGDSVVVFAQGPIGLCATAGAKLMGAALVIGVEGDATRHAVARQMGADI
                     VLDPTKVDVVAEVKRLTNGGADVAIEALGLQETFESALRAIRPGGTLSSLGVYSGKLQ
                     MPYDAFAAGLGDHTIVTTLCPGGKERMERLMRVVASGRFDPLPLITHRFSLDEIGEAY
                     RVFSNRLDGVLKVAIRP"
     gene            2441..3619
                     /locus_tag="VIN32_04830"
     CDS             2441..3619
                     /locus_tag="VIN32_04830"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HEY8921937.1"
                     /translation="MASRSIQESDAQALPPALQALDAAVRGISGVLDVEQVLQLIVDK
                     VRELVSAQYAALGIVDEAGLITQFITSGITVEEREAIGDLPRGHGLLGLIIRENRSYR
                     IPNIASHPESYGFPANHPPMASFLGAPATIRGRVVGRLYLTNKQNAAEFSADDQALVE
                     MFALHAAIAIDNARLYEQVQRLAVVDEQDRISRELHDSIIQSIYAVTLSLDDVPELVG
                     EAPAGAGQRVDDAIDALHGVIRDIRNFIFGLRPLLLDSGSLLDGLRTLADELRRNTTT
                     EIDIQGKEPEGISLEIVAELLAISREALSNVARHSGAAHTSLRVTAHDTDVRLEITDD
                     GRGIDANPAPAGGHHGLANMRTRAEALGGTFDVDSSPGAGTRIIVSIPRTGSRQGASQ
                     "
     gene            3616..4293
                     /locus_tag="VIN32_04835"
     CDS             3616..4293
                     /locus_tag="VIN32_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007621541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY8921938.1"
                     /translation="MTSEQPARPLRLLVVDDHEVVRQGLVSLLDRRSGFEVVAQAGSI
                     AESITMAARYEPDLVIMDVRLPDGSGIEACREIRAARPETRVVMLTSYPDEEAVLSAI
                     IAGASGYLLKQIRGRDLVSALEAVGRGESLLDSAVTEKVLQRVRRMASGSATDDLAEL
                     TSQERKILLLVAEGKTNKEIATDVFLSDKTVKNYVSSILAKLNLQRRTQAAAYVAKRH
                     LGSPDDH"
     gene            4377..5093
                     /locus_tag="VIN32_04840"
     CDS             4377..5093
                     /locus_tag="VIN32_04840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921939.1"
                     /translation="MDYRSTRGSQFMISRLRGLGFVLALIGIAFIAAGGYAFIKTQEG
                     YRSLNAFSAAQDVQLTYNDKGELVDRGETEGAQAIMALLTKDWHYPVVGSELDPNDPI
                     VNTASEYMYQMATVSYHTLHGTQTVVLTEPAEYKGETFPAGTYDVPIDGRYFSQLDRQ
                     HPLEGPARDQAWSGTAHALVAELGVGTATASALQLALALAALLGGLGGTLILTGAGLV
                     WATRSETQPVQVLPPPDGPA"
BASE COUNT          889 a         1735 c         1751 g          870 t
ORIGIN      
        1 gccccggagg gttcaattgg agagctgcag acctgccacc tttggggtga tcggtgaggg
       61 gcgcaaaccg gaggtcaccg ttgccgtcgc gtggctccac cgccgctcag gcgcctggtc
      121 ggaggtccgg atcctctaac agggtccgaa gcaggtcgag ggagctgatt acgccgatcg
      181 gcacctcctc gtcatcgacc acgaccaggc ggtggatgtg ccgcgtaacc atcagctggg
      241 ccgcacgttt cagcgacgta ccggtgcgga cggtcacggc aggcgtggtc atcaggtgcc
      301 tgacctgcag tcttgcccag ttcccgctga tcatcgcgct cgatcgcgcc atcgccaggt
      361 ccgtctggct gatgacgccc accgtcgcgt tcccactgac gacgggcagg cccgacacac
      421 ggtaggtctt cagcaggcgc tcggcttcgg tggcctgcgc gtcggcgccg ataacgaccg
      481 ggtcgttgga catcaggtcg ccaacccgaa gatcgttcgc tgccgtagtc atggttgctc
      541 ctccgggcat gccttaggcc ggcgctcgaa gcggaccggc ctgtcggcag ccttcgcctt
      601 accgcctgat gatcaccgtt tcggtggcgt gggtcgcgac gtgctcggac acactgccct
      661 gcagcacgcg ctccagccta ccaagcccac gactcccgat cacgatcgtg tcgtagccgc
      721 tttccgcggc cgttcgctcg atcgcgcgcg cggggtcgcc catcggctca tgcagctgcg
      781 cctcgatgcc ggccttcgcc agaatttgct gggcctgccc cagctgggcg gcgtgaatcg
      841 gtcgatcgtc ccacgggtcg atcggtgctc gacccgcatg gatgggaacc acgctcacga
      901 cgccaacagc ggcgtcaaat gccttcgcca agtcgatcgc agtggccagg gcatgttcgg
      961 cgggctccgt gccgtcatat gcgacgagga tctttttcat ctcccggact cctttccgtc
     1021 gaggggctcg cctaaaccct ggggttacgc ggggttacgc gcgcgagccg gctcggtccc
     1081 gatggtggac tggccatggc ttgcgcgaac agggccatcg gacccgactc aacaggacct
     1141 ccggcagtag ggcgtgcagt tctgccttgc caggatgagg gcgacgacag aacgagccgc
     1201 actaacgacg attcgccgga ggtagttcgt cgtccagaga ggagagatca cgatggtcac
     1261 tgcatcgcga gccagcgtcg caaccgccct ggaggcggcc accatgcggg cggcggtctt
     1321 ccatgcgccg ggcgacatcc gtgtcgagaa ggtcgcgcgc ccccggcccg gcccaggtga
     1381 ggcggttatc cgcgtgacgc tgacgacaat ctgcggcacc gacgtccaca ttctcaaggg
     1441 cgagtatcgc gtggagcctg gtttgatcgt cggccacgag ccggtcggaa tcatcgagga
     1501 gctcggcccg ggtgtggtcg gctacaacgt cggggagcgc gtcctggtcg gggccatcac
     1561 gccgtgtggc caatgccacg cgtgcctgag cggcaagctg agccagtgcg gccatggcac
     1621 cggctatgag gctatcggcg gctggcgttt cggcaatacc atcaacggtg cgcaggccga
     1681 gtccctcctg gtgccgtccg cccaggccaa cctggcaaag atccccgaca ccctgaccga
     1741 cgagcaggtt gtcctgctgg ccgacatcgc ctcgaccggc ttcgcgggcg cggagtccgg
     1801 caaggtgcgc atcggcgact cggtggtcgt cttcgcgcag gggccgatcg gcctgtgcgc
     1861 caccgcgggc gccaagctca tgggggcggc cctggtgatc ggcgtggagg gtgatgcgac
     1921 ccgccatgcg gtggcgcgcc agatgggtgc cgacatcgtc ctcgatccaa cgaaggtcga
     1981 cgtcgtagcc gaggtcaagc gattgacgaa tggcggtgcc gatgtggcga tcgaggcgct
     2041 cgggttgcag gagaccttcg agagcgcgct gcgggccatt cgaccgggtg ggacgctgtc
     2101 gagcctcgga gtgtactcgg gcaagctcca gatgccgtac gacgcgttcg cggcggggct
     2161 cggcgatcac accatcgtca cgactctctg ccccggtggc aaggagcgca tggagcgcct
     2221 catgagggtg gtcgcctccg gccgcttcga cccgctgccg ctgatcaccc accgcttctc
     2281 gctggacgag atcggcgagg cgtaccgcgt cttcagcaat cgactggacg gcgtcctaaa
     2341 ggtcgccatc cgtccttagc gggcgtgtcg ggcgatccta cggcgggcat cgggcctatc
     2401 cgaagcgttc ccggtgcgac gatcggggta gcctatccgc gtggcgagcc gttcgattca
     2461 ggaatccgac gcgcaggccc ttccgcccgc cctccaagcg ctcgatgccg ccgtccgggg
     2521 catcagcggc gtgctggacg tcgagcaggt cctgcaactg atcgttgaca aggtccgaga
     2581 gctggtctcg gcccaatacg cggcgctggg catcgtcgac gaagcgggcc tgatcaccca
     2641 gttcatcacc agcggaatca ccgtcgaaga gcgggaagca atcggcgacc tgccacgcgg
     2701 ccatggcctc ctcggtctga tcatccggga gaatcgctcg taccgaatcc cgaacatcgc
     2761 atcccatccg gagagctacg gcttcccggc caaccatccg ccaatggctt ccttccttgg
     2821 cgcacccgcc accatccgcg gccgggtcgt ggggcggctc tatctgacaa acaagcagaa
     2881 cgccgccgag ttcagcgccg atgaccaggc gttggtcgag atgttcgcgc tgcacgctgc
     2941 catcgccatc gacaacgcgc gcctctacga gcaggtccag cggctggcag ttgtggacga
     3001 gcaggaccgc atcagccgtg agctgcacga cagtatcatc cagagcatct acgccgtgac
     3061 attgtcgctt gacgatgtcc ccgaactggt cggcgaggct cccgccgggg ccggccagcg
     3121 cgtcgacgat gcgatcgatg cgctccacgg ggtgatccgc gacatccgta acttcatctt
     3181 cggcctccga ccgctgctgc tcgattcggg cagcctcctc gatggactcc ggacattggc
     3241 agatgagctg cgccgcaata cgaccaccga gatcgacatc caggggaagg aaccggaagg
     3301 catctcgctg gaaatcgttg cggagctgct tgccatctca cgcgaagcgc tctcgaatgt
     3361 cgcgcgccat tcgggcgcgg cccatacctc gctgcgggtt accgcgcacg acaccgatgt
     3421 gcggctcgag attacggatg acggccgcgg catcgacgcg aaccccgcgc ctgcgggcgg
     3481 ccatcatggg ctggcaaaca tgcgaacgcg ggccgaagcc ctcggtggca ccttcgacgt
     3541 cgacagtagc cccggtgccg ggacccgtat catcgtttca attccgcgca ccggatcgcg
     3601 gcaaggagcc agtcaatgac gtccgagcag ccggcacggc ccctgcggct cctggtcgtc
     3661 gacgaccacg aggtcgtgcg ccagggtctt gtgtccctgc tcgatcgtcg gagcgggttc
     3721 gaggtcgtcg cgcaggccgg ttcgatcgcc gagtcgatca cgatggccgc gcggtacgag
     3781 ccggatctgg tgatcatgga tgtacgcctg cccgacggct cgggcatcga ggcctgccgc
     3841 gagatcagag ccgcccggcc tgaaacgcgg gtcgtgatgc tgaccagcta ccccgacgag
     3901 gaggccgtgc tgtccgcgat catcgcgggc gcaagcggct atctgctcaa gcagatccgc
     3961 gggcgcgacc tggtgagcgc gctcgaggcg gttggacgtg gagagtcgct gctcgattcg
     4021 gccgtgaccg agaaggtgct ccagcgagta aggcgcatgg ccagcgggtc cgccacggat
     4081 gacctcgccg agctgaccag ccaggagcgc aagatcctgc tgctggtggc ggaggggaag
     4141 accaacaagg agatcgcgac ggacgtcttc ctgtccgaca agaccgtgaa gaactacgtc
     4201 agcagcatcc tggcgaagct caacctccag cgccggaccc aggcagcggc ctacgttgcc
     4261 aagcgccatc tcgggtcgcc agacgaccat tagcccggag ccgtagggcc ggctggccga
     4321 caaataggtc cgtatggcac gtgggttgca cccgactgac gaacgagagt tgctgcatgg
     4381 attaccgcag cacaagaggt tcacagttca tgatcagtcg gttgagagga ctcggattcg
     4441 tcctcgcact catcggcatt gcgttcatcg cggccggtgg atatgcattc atcaagacgc
     4501 aggaggggta tcggtccctc aatgccttca gcgctgcgca ggacgtccag ctgacctaca
     4561 acgacaaggg cgagttggtg gaccgcggcg agacagaagg cgcgcaggcc atcatggccc
     4621 tgctgaccaa ggactggcac tacccggtgg tgggctcgga gctcgacccc aatgacccga
     4681 tcgtcaacac cgccagcgag tacatgtacc agatggcgac ggtcagttac cacacgctgc
     4741 acggcacgca gaccgtggta ttgacggagc ccgcggagta caagggcgag acgttccccg
     4801 ccggcaccta cgacgttccg atcgacgggc ggtacttctc ccagcttgat cggcagcatc
     4861 cgctcgaggg cccggcccgc gatcaggcgt ggagcggcac cgcgcatgct ctggtcgcag
     4921 agctgggcgt cggcactgcc accgcatcgg ccctacagct cgcgctggcg ctggccgctc
     4981 tgctcggcgg actgggcggg accctgatcc tgacgggcgc tggcctggtg tgggccaccc
     5041 ggtccgagac gcagccggtc caagtgctgc cgccgcccga cgggccggcc tgacgggacg
     5101 ccgcacatcg cgggtcccgt cacccggccg ttaccccggg aagcctgatc caaacggcga
     5161 gcgaccaact cgccggggtc aggctcctgg ctcttctgcc aagaaacacg aggtgcagcg
     5221 atgcggattg tgatgggggt tgatg
//
LOCUS       DATCQR010000145         3064 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_117067, whole genome shotgun sequence.
ACCESSION   DATCQR010000145 DATCQR010000000
VERSION     DATCQR010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3064
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117067"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..128)
                     /locus_tag="VIN32_04845"
     CDS             complement(<1..128)
                     /locus_tag="VIN32_04845"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007287624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class II"
                     /protein_id="HEY8921940.1"
                     /translation="MDRNLALELLRVTEAGALAAAPLQGRGDKMGADGRAVEAIRNA"
     gene            complement(190..318)
                     /locus_tag="VIN32_04850"
     CDS             complement(190..318)
                     /locus_tag="VIN32_04850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921941.1"
                     /translation="MRSDIPLGEDVYLDGGGDRQLAGNNDLCVEASQLPIRQRGIS"
     gene            complement(373..867)
                     /locus_tag="VIN32_04855"
     CDS             complement(373..867)
                     /locus_tag="VIN32_04855"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEY8921942.1"
                     /translation="METVAISGSNGGPDPYGIEPKSTILGRDDVLSGKLQLKSGGQIL
                     GNFSGQVESDGDLWIGSEAHVEADIRSDRVTIAGFVRGNIVAASRLKITSTGRLEGDA
                     KVGALVVQEGGVHHGVIRVHPEGVPEHEEKIVVDAAARPAVVAVKNVPNPVGRVRKLW
                     GEFF"
     gene            complement(1017..2366)
                     /locus_tag="VIN32_04860"
     CDS             complement(1017..2366)
                     /locus_tag="VIN32_04860"
                     /inference="COORDINATES: protein motif:HMM:NF013871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEY8921943.1"
                     /translation="MGADQPIDDRELLRLLKRAAADRGAKAWVVGGYVRDHLLGRAQP
                     DLDVVVEEGGASQLAERFADLAGAGPPVTFERYGTAQVTLPGHLVEFVSARSESYASD
                     SRKPDVRPATLEEDLRRRDFTINTLLMDFGGEIQDRLGTARADLEARILRTPADPLRT
                     FSDDPLRMLRAVRFAAQLGFELAPDLLPAMRQLSGRLAPPVVSVERITDELCKMLESE
                     RPKLALELLDSGGLLEVVLPEVTACKGVEQGGYHTHDVFGHTLLAVGLAPPDLVVRLA
                     ALLHDVGKPVTATPDGAFTGHEEVGAGIARGALERLRFPQREIDAVTTLVRLHLRPVY
                     YRSEWSDGAVRRLARDAGPLIERLMALARADIGASAYPEPEKLDELAARLATVLAEQP
                     TRMAAPIDGEDIMRVRGLAPGPEVGRIKERLGELIMDGEIEPTREAVLAYLQSHGDL"
     gene            complement(2345..>3064)
                     /locus_tag="VIN32_04865"
     CDS             complement(2345..>3064)
                     /locus_tag="VIN32_04865"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEY8921944.1"
                     /translation="GREKVRSIAIGAPGPIDIKRGVLVNPPNLPGWRNVPLAAMLREA
                     TGAVAHVANDADMAGLGEFHRGAGRDTRNMVYITWSTGVGGGLIVDGKLHRGGHGTAG
                     EVGHMIIDANGPLDQCGQHGCLEVFCGGANLARETGHPAAELFAAAARGDKHAGLVVE
                     RAARYMGKALISLTNVIDPEVFVMGGGVTRSWGLVQPLLLETLRSSPFIRPARRPVLR
                     RARLGDRAGQVGAVEWARINQ"
BASE COUNT          480 a         1097 c          982 g          505 t
ORIGIN      
        1 gcgttgcgga tggcctcgac agcgcggccg tcggcgccca tcttgtcgcc gcggccctgc
       61 aatggagcgg cggcgagggc tccagcctcg gtgacgcgga gcagctcgag ggctaggttt
      121 cggtccatct cccgatccac ctagccgcca gcccccgtcg acatcgcgcc gggtagcgta
      181 tccgaatccc tagctgatgc cgcgctgccg gatcggcagc tggctcgcct ccacgcagag
      241 gtcgttgttg ccggccagct ggcggtcgcc gccgccgtcc aggtacacgt cctcgccgag
      301 aggaatatcg cttcgcatcg gtagcgcgta gcgctggcgc ggttggctct ggatggagtt
      361 ccgggcgcgc gcctagaaga actcacccca caacttcctt acccggccga ccgggttagg
      421 gacgttcttg accgcgacga ccgccggtcg cgcggccgcg tcgacgacga tcttctcctc
      481 atgctcaggc acaccctcgg ggtgaacgcg gatcaccccg tggtggacgc ccccttcctg
      541 gaccaccagc gcgccgacct tggcatcgcc ctcgaggcgg ccggtgcttg tgatcttgag
      601 ccggctcgca gcgacgatgt tgccgcgcac gaacccggcg atcgtcaccc tgtcgctgcg
      661 tatgtcggcc tcgacatggg cttcggagcc gatccacaga tctccatcgg actcgacctg
      721 gccgctgaag ttgcccaata tctggccgcc gctctttagc tgcagcttcc ctgacagcac
      781 gtcatcgcgg ccaagaatcg tcgacttcgg ctcgatgcca taggggtcgg gtcccccgtt
      841 actcccggaa atcgcgaccg tctccacccc aatgtccgtc atctggacct cccttaccca
      901 gccccccggc tgttcccaat ccgcagcata gaggctacgc agtcagggtg aatcgtgcaa
      961 tttcctcccc cttggcggga gggcagggtg ggggggtcgg ggcctgcacg cactgttcag
     1021 aggtcgccat gtgactgcag atacgccagg acggcctcgc gcgtgggctc gatctccccg
     1081 tccatgatca gctcgcccaa acgctccttg atccgcccca cctcgggccc cggcgccagg
     1141 ccacggactc gcatgatgtc ctcgccgtcg attggcgcag ccatccgcgt cggctgttca
     1201 gccagtacgg tcgccaggcg cgccgccagc tcgtcgagct tctccggctc tggataggcg
     1261 gacgccccga tatctgcgcg cgcgagcgcc atcagccttt cgatcagagg gccggcgtcg
     1321 cgggcgagcc gccgaacggc cccgtcgctc cactccgagc ggtagtacac agggcgaagg
     1381 tgcagccgga ccagggtcgt aacggcatcg atctcccttt gagggaatcg aagccgttcg
     1441 agcgcacctc tcgcgattcc ggcgccaacc tcctcgtgac cggtgaaggc cccgtccggg
     1501 gtggcggtga ccggcttccc cacatcgtgc aggagcgcgg ccagcctcac cacgaggtca
     1561 ggcggcgcga gtccaaccgc gagcagcgtg tggccgaaaa cgtcgtgggt gtgataacca
     1621 ccctgctcaa cacccttgca ggcggtcacc tcgggcagca cgacctcgag cagtccaccc
     1681 gagtcaagca gctcgagcgc cagcttgggc cgctcggact cgagcatctt gcagagctca
     1741 tcggtgatgc gctcgaccga aaccaccggc ggcgccagcc ggccgctgag ctgccgcatc
     1801 gccggcagca ggtccggcgc cagctcgaag ccgagctgag cggcgaatcg cacggcacgc
     1861 agcatccgca gggggtcgtc gctaaacgtc cgaagcgggt cggccggagt gcggagaatg
     1921 cgcgcctcga ggtctgcgcg agctgtcccg agccgatcct ggatctcgcc gccgaagtcc
     1981 atcaacagcg tgttgatggt gaaatcgcgc cgccgcaagt cctcctcgag ggtggctggg
     2041 cgcacgtcag gctttcggga gtcgctcgca tacgactccg accgggctga gacaaactcg
     2101 accaggtggc cgggcagcgt cacctgggcg gtgccgtatc gctcaaaggt gacaggcggg
     2161 cccgcgccag ccagatccgc aaagcgctcg gccagctggc tggccccgcc ctcctcgacg
     2221 accacgtcga gatcgggctg cgcgcggccg agcagatggt cgcggacgta gccgccgacg
     2281 acccaggcct ttgctccccg gtcagcggcg gctcgtttga ggagacgcag cagctcccga
     2341 tcgtctattg gttgatccgc gcccactcga cagctcccac ctgcccggcg cgatcaccaa
     2401 gccgggctcg gcgcagcacc ggccttcgtg cgggtctgat gaatggagat gagcgcagcg
     2461 tctccagaag cagcggctgc accaggcccc aggaacgcgt gaccccgccg cccatcacga
     2521 agacctccgg atcgatgacg ttcgtgaggc tgatcaaggc ctttcccatg taacgcgctg
     2581 cccgctcgac gaccagcccg gcgtgcttgt cacctcgcgc ggccgctgca aacagctccg
     2641 cggcagggtg gccggtctct cgcgcgaggt tcgcaccgcc gcagaagacc tcgagacagc
     2701 cgtgctgtcc gcattggtcg agcgggccgt tggcgtcgat gatcatgtgc ccaacctcgc
     2761 ctgccgtccc atgcccgccg cggtgcagct tcccgtcgac tatcaatccg ccacctacgc
     2821 ccgtcgacca ggtgatgtag accatgttcc gggtgtcccg tccggcgccg cgatggaact
     2881 ctccgagccc cgccatgtct gcgtcgttgg ccacgtgcgc gacagcgccg gtggcctctc
     2941 tcagcattgc cgcgagcggg acgtttcgcc aaccaggaag gttgggtggg ttgacgagaa
     3001 cgcctcgctt gatatcgatc gggccagggg cgccgatggc gatgctgcgg accttctcgc
     3061 gacc
//
LOCUS       DATCQR010000146         4394 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_8328, whole genome shotgun sequence.
ACCESSION   DATCQR010000146 DATCQR010000000
VERSION     DATCQR010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4394
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8328"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..975)
                     /locus_tag="VIN32_04870"
     CDS             complement(<1..975)
                     /locus_tag="VIN32_04870"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY8921945.1"
                     /translation="MIGRLIGGRYRVIAPLGEGGMATLWRAMDEQLDREIALKLLRPQ
                     YGNDPGFAARFKQEARSAGSLSHPNIVSVYDYGTDAETGSQFIVMQLVDGEDLAAILH
                     KHGRLETDDAVRIAIGVASALEAAHRRGIVHRDVKPGNILIDQDVKVTDFGIARAVSE
                     ASMTVTGTTLGSVHYFSPEQARGDEVTGQSDVYALGIVLYEMLTGRRPFEGDSAAGVA
                     LKRLSEDPAPPTDHGPVPPGLSAIVMRALERDPAKRYPDAGAFAEALRVWQHDPDAAN
                     AVAAVAVPTTPSAGEPTVYVPPRVTLPSDRAAISGMPANGAGSRGAPPG"
     gene            complement(988..1860)
                     /gene="galU"
                     /locus_tag="VIN32_04875"
     CDS             complement(988..1860)
                     /gene="galU"
                     /locus_tag="VIN32_04875"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008824754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEY8921946.1"
                     /translation="MRVRKAVLPAAGFGTRFLPATKAIPKEMIPLVDRPIIQYAVEEA
                     VASGIEQIIIVIASGRSAVEEHFDSHPGLESWLEERGDIEMLRVVRRISEIGPIAFVH
                     QKEQLGLGHAVLMAKELVGDEPFAVLLSDDVMLNPGGKPVTEQLIEQYQAHRGSMIAV
                     QRVPRDDVDRYGIVAPLHQEGRLVEIDDLVEKPSVADAPSDLAVLGRYILSPKIFDKL
                     ETTQRGAGGEIQLTDAIRALSAEQPVFGYQFEGVRHDAGTRLGWLKANIAMSLESDLA
                     DEVRAHVRSLDLRD"
     gene            2021..2503
                     /locus_tag="VIN32_04880"
     CDS             2021..2503
                     /locus_tag="VIN32_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921947.1"
                     /translation="MINSLIRSLKGPSRDPRMALISLPLPRNLPDEQVLAALEIALAA
                     DPNPDDLVETLPAALRQVTGHDYQVLDRAASDVTGSFRRSQVMIRDGAVGLWPGYAAR
                     AYPLLAPSPHAERTRAAIVAADGSPADAPGPTNPVGKPGWAPPPVRRKRADETDAESA
                     "
     gene            2504..2683
                     /locus_tag="VIN32_04885"
     CDS             2504..2683
                     /locus_tag="VIN32_04885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921948.1"
                     /translation="MGRQNVPRDQWLEIGAECPHCGERVTEEMVYSWRPVPDDPKLLL
                     LWCPDCGDRVEINHV"
     gene            complement(2702..2778)
                     /locus_tag="VIN32_04890"
     tRNA            complement(2702..2778)
                     /locus_tag="VIN32_04890"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2742..2744),aa:Glu,seq:ttc)
     gene            complement(2828..3154)
                     /locus_tag="VIN32_04895"
     CDS             complement(2828..3154)
                     /locus_tag="VIN32_04895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921949.1"
                     /translation="MQASRMHPFLRNVVIGVVGLLIAAGLTAMSVLSADTAFSVAAML
                     VSALIAVVIGVFLFVQGWIWSQRAYRTRSTGMSVAIALGGGFMILLAALALAGAVILV
                     ILFYLP"
     gene            3201..>4394
                     /locus_tag="VIN32_04900"
     CDS             3201..>4394
                     /locus_tag="VIN32_04900"
                     /inference="COORDINATES: protein motif:HMM:NF013252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HEY8921950.1"
                     /translation="MSSSPFAAFAETAEAVADTTSKLRKRDLLAAYLRGLPAGDVPIA
                     ATFFAGRPLPGSADKLGLGWVQQTQALAAASGADEEALHEAYLRHSDFGDAAAELLSG
                     RRPAGTPLTIADVEAAFRSMSEATGAAGRVTLMTELFARASAAEARFVGRIAARELRI
                     GLREGLLEEAIASAFERPIEAVRRGVMLVGEPGEAAVLARDDRLAEAALHLGRPIRFM
                     LATPVADADEVMRRVGDEAWIEDKYDGIRAQLHLDPPAPPRLFSRDLNEIGRAFPEVV
                     AAATEFGHRLVVDGELVPYRSGAVLDFASLQTRLGRVDPSSELLEEVPVVLVAFDLLH
                     LDGRDLLETPLRERRAALEELRLPERSAERFLYSQLATARSADEVEAHFDDARERRNE
                     GLMVKD"
BASE COUNT          653 a         1527 c         1564 g          650 t
ORIGIN      
        1 gcccggtggt gcgccacgcg agccggcgcc gttggccggc atgccggaga tggcggcgcg
       61 atccgacggg agcgtgaccc gaggcgggac gtagacggtc ggctcgccgg ccgatggcgt
      121 ggtggggacg gcgacagcgg cgacggcatt ggcggcatca gggtcgtgct gccagacacg
      181 gagcgcctcg gcgaacgcgc ccgcgtccgg gtaccgcttg gcgggatcgc gttccagcgc
      241 acgcatcacg atcgccgaca ggcccggtgg caccgggcca tggtcggtcg gcggcgccgg
      301 gtcctcgctc agccgcttca gggccacgcc ggcggccgag tcgccctcga acgggcggcg
      361 accggtcagc atctcgtaca ggacgatccc cagggcgtag acgtcggact ggcccgtcac
      421 ctcgtcgcca cgcgcctgct cggggctgaa gtaatgaacc gatccgagcg tggtgccggt
      481 gaccgtcatg gaggcctcgc tcacggcccg ggcgatcccg aagtcggtca ccttcacgtc
      541 ctggtcgatc aggatatttc ccggcttcac gtcgcggtgc acgatcccgc gccgatgcgc
      601 cgcctcgagg gcggacgcga caccgatcgc gatgcgtacc gcgtcatcgg tctccaggcg
      661 gccatgcttg tgcaggattg cggccaggtc ctcgccatcg accagctgca tgacgatgaa
      721 ctggctacct gtctcggcat cggtcccgta gtcgtagacc gacacgatgt tcggatggga
      781 gagcgacccg gccgatcgcg cctcctgctt gaagcgtgcg gcgaagccgg ggtcgttgcc
      841 gtactggggc cggagcagct tgagggcaat ctcgcggtcg agctgctcgt ccatggcgcg
      901 ccacagggtc gccatgccgc cctccccgag gggggcgatg acccgatatc gaccgccgat
      961 cagccgcccg atcatctcca ccgcgggtca gtctcgcagg tcgaggctgc gcacatgcgc
     1021 ccgaacctcg tcggcgaggt cggactcaag ggacatcgcg atgttggcct tcagccagcc
     1081 gagtcgagtg cccgcgtcgt ggcgcacccc ctcgaactgg tagccgaaga cgggctgctc
     1141 ggcggagaga gcgcggatgg cgtcggtgag ctggatctcc ccgcctgccc cgcgctgggt
     1201 cgtctccagc ttgtcgaaga tcttggggct gaggatgtag cgcccgagca ccgcaaggtc
     1261 ggacggagcg tcggcgaccg atggcttctc gaccaggtcg tcgatctcga ccaggcgccc
     1321 ctcctggtgc aggggcgcca cgatcccgta gcggtcgacg tcgtcgcgcg gcacgcgctg
     1381 gaccgcgatc atcgagccgc ggtgcgcctg gtactgctcg atcagctgct cggtgacggg
     1441 cttcccgccg ggattgagca tcacgtcgtc ggagagcagg acggcgaagg gctcgtcgcc
     1501 gaccagctcc ttcgccatca gcaccgcatg ccccaggccg agctgctcct tctggtggac
     1561 gaacgcgatg gggccgatct ccgagatgcg acgaaccacg cgcagcatct cgatgtcgcc
     1621 gcgctcctcg agccagctct ccaggccggg gtgcgagtcg aagtgctcct cgacggcgct
     1681 ccggccgctg gcgatgacga tgatgatctg ctcgatcccc gaggcgaccg cctcctcgac
     1741 cgcgtactgg atgatcggtc ggtcgaccag cgggatcatc tccttgggga tggccttggt
     1801 cgcgggcagg aagcgggtgc cgaagccggc cgccggcagg acggcctttc tgacgcgcac
     1861 ggtgatcagg tctctcctcg ggcgggttgt gtctggcgca gcgcgctggc gtttctgggg
     1921 ctgcgcctac gaagggtgtc tcactgggtg aaggagtata gcaacgccac cggcaccgcc
     1981 cgcgaaggcg gtaggtccac gacgctacaa tcgcaaaccg atgatcaact ccctgatccg
     2041 cagcctgaag gggcccagcc gcgacccgcg catggccctg atcagccttc ctctgccgcg
     2101 caacctgccg gatgagcagg tgctggctgc gctggaaatc gccctcgctg ctgaccccaa
     2161 ccccgacgac ctggtcgaga ccctgcctgc tgcgctgcgc caggtgacgg gccacgacta
     2221 ccaggtcctc gaccgggccg cgagcgacgt gaccggcagc ttccgacgct cgcaggtgat
     2281 gatccgtgat ggtgccgtcg gcctgtggcc gggctatgcc gcgcgggcct acccgctgct
     2341 cgccccgtcg ccgcatgccg agcgcacgcg ggccgcgatc gtggccgcgg acggctcacc
     2401 ggccgatgcg ccgggcccca ccaacccggt cggcaagccc ggctgggcac cgcctccggt
     2461 ccgtcgcaag cgggccgacg agactgacgc ggagagcgcc taggtgggtc gccagaacgt
     2521 cccgcgcgac cagtggctgg agatcggcgc cgaatgtccg cactgcggcg agcgggtcac
     2581 ggaggagatg gtctactcgt ggcgtcccgt gcccgacgat ccgaagctgc tgctgctgtg
     2641 gtgccccgac tgcggagacc gggtcgagat caaccacgtc tgagcgctcg tccgggtcgt
     2701 gtggtgcccc caaccggatt cgaaccggtg atctccacct tgaaagggtg gcgtcctagg
     2761 cctctagacg atgggggcgc tggctctccc atcgtagcaa ggcgcccggc cgtcccattg
     2821 ccgccgccta cgggaggtag aagaggatca ccaggatcac ggccccggca agggccaggg
     2881 ccgccagcag gatcatgaac ccgccgccca gcgcgatcgc caccgacatg ccggtcgagc
     2941 gggtgcggta ggcgcgctgc gaccagatcc agccctgcac gaacagaaag acgccgatca
     3001 cgaccgcgat cagcgccgac accagcatgg ccgccaccga gaacgcggtg tccgcgctga
     3061 gcacggacat ggcggtcagg ccagcggcaa tcagcaaccc gaccacgcca atcaccacgt
     3121 tgcgcaggaa ggggtgcatc cgcgaagcct gcatggggcg atcttcctcg tcaaggcggc
     3181 ccgctcgtag acttgcgggc atgtcctcaa gcccgttcgc cgcgttcgcc gagacggcag
     3241 aggcggtcgc cgacacgacc tccaagctgc gcaagcgcga cctcctggcg gcctacctgc
     3301 gtggccttcc ggccggcgac gtgccgatcg ccgccacctt ctttgccggc cggccgctcc
     3361 ccggcagcgc cgacaagctg gggctcggct gggtgcagca gacccaggcc ctcgccgcag
     3421 cgagcggcgc cgacgaggag gcgctgcacg aggcgtacct gcgccattcc gacttcggcg
     3481 atgccgccgc agagctgctc agcggacgga ggccggccgg cacgccactg acgatcgccg
     3541 acgtcgaggc cgccttccgg agcatgagcg aggcgactgg cgccgcgggg cgcgtgacgc
     3601 tgatgaccga gctgttcgct cgtgccagcg cggccgaggc gcgcttcgtt gggcgcatcg
     3661 cggcgcgcga gctgcgcatc gggctgcgcg agggtctgct cgaggaggcg atcgcctccg
     3721 cattcgagcg gccgatcgag gcagtgcggc gtggcgtgat gctggtcggc gagcccggag
     3781 aggcagccgt gctggcgcgt gacgaccggc tcgccgaggc ggctctgcac ctggggcggc
     3841 ccatccgctt catgctggcg acgccggtcg ccgacgcgga cgaggtgatg cgccgggtgg
     3901 gggacgaggc ctggatcgag gacaagtacg acggcattcg cgcccagctg cacctggacc
     3961 ccccggcgcc gccccgcctc ttctcgcgag acctcaacga gatcggccgc gccttcccgg
     4021 aggtcgtggc cgcggcgacg gaattcgggc atcggctggt ggtcgacggt gaactggtgc
     4081 cgtatcggtc cggcgcggtt ctcgacttcg ccagcctgca gacgcgcctc gggcgcgtgg
     4141 atccgagctc tgagctgctc gaggaggtgc ccgtggtcct tgtggccttc gacctgctgc
     4201 acctcgacgg ccgggacctg ctcgaaacgc cgctgcgcga gcgtcgggcc gcgctggagg
     4261 agctgcgcct gccggagcgt agcgctgagc gcttcttgta ctcgcagctc gccacagcgc
     4321 gctccgccga cgaggtggag gcccatttcg acgacgcgcg ggagcgacgc aatgagggcc
     4381 tgatggtgaa ggac
//
LOCUS       DATCQR010000147         6185 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_193667, whole genome shotgun sequence.
ACCESSION   DATCQR010000147 DATCQR010000000
VERSION     DATCQR010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6185
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193667"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..617)
                     /locus_tag="VIN32_04905"
     CDS             complement(<1..617)
                     /locus_tag="VIN32_04905"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HEY8921951.1"
                     /translation="MSVHAGTADRHEHLDHMGLGEQDPDAARGNMLLAVKLGILSEVM
                     LFGALFAAYFVIRSESPGWPPVAGLERPALFLPGLNMLLLVASSGTMQWTVRSARSGD
                     QSRIRRWTRLTLLLGTIFIVIQGYEFATNGFGLDAGTFGSTFYVLTGFHAAHVLAGLG
                     LMAIVATRRRRGLVSAEHHTIVEATSYYWHFVVAAGVFLFLTLYVL"
     gene            complement(614..2452)
                     /locus_tag="VIN32_04910"
     CDS             complement(614..2452)
                     /locus_tag="VIN32_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011142160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEY8921952.1"
                     /translation="MRALKPGLLRAVLGAAIGAAIGAGSLILAGSLGMEITDGEILGL
                     GYLLALIGFLLGIGAFRFWLIWATGREVNAQEEHAAHGTAGDWRRYFRFTTDHKVIGV
                     QYLATAFALFLVAGTFAMLMRLELAQPGLLLGKEAYNEIFSTHGAMMITVALISIVGG
                     LGNYLVPIMIGARDMAFPKSNAFSYWLLPLPILLVAINPLLGGFDSGWTAYPPLSQQA
                     NLGQQAYLMAFVTVGVASILSSINFLVTVFRMRAPGMSLMRMPIFVWALTVTSVLGLV
                     ATSIVASAFTMLLFDRVLGTSFFRASRGGDVILFQHLFWFYSHPAVYIMGLPGLGAIL
                     EIVPVFARKPLFAYPLAVLSFISIGVMSFMVWAHHMFVSGMATFFPIPIMLTTELISV
                     PTGIVFLSALGTIWMGRLHFKVPMLWVFGFIWAFLIGGITGVFLADVPTDLTLHDTYF
                     VVAHFHYTIVGGAIFGLFAGTYYWFPKITGRMYSERLGKLHFWWFTLAFNATFLPMFW
                     LGIEGMRRRVADYPAQFGGVNLWISFAAFNIFLSVAVFVFNIVRSWRRGPQAASNPWR
                     AQTLEWQTASPPPIQNFDHIPTVTTGPYEYGSGPLIGVPAEGRSTP"
     gene            complement(2449..3204)
                     /gene="coxB"
                     /locus_tag="VIN32_04915"
     CDS             complement(2449..3204)
                     /gene="coxB"
                     /locus_tag="VIN32_04915"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEY8921953.1"
                     /translation="MTSPRRHLWIAAAIWAVLSIVGAVLVSGIQIMPVIASREAVIEN
                     SAFVLLTVLSVPVLMFVVVGLAYSAIWFRADGDEADGPPIHGHRGFQTVWLGISFALV
                     VGLFGYGAVGLIDIRGAQVSDFEVQVRAEQWAWHFTYPSAGVDSQELHIPVGERVHFI
                     INSDDVIHSFWVPAFGIKQDAVPGRTTQIYVTATVAGTYPGLCAELCGLGHTDMRFTT
                     VVNDRAALEAWLRQSTPSPAGSQPPASGSEQPS"
     gene            complement(3221..3682)
                     /locus_tag="VIN32_04920"
     CDS             complement(3221..3682)
                     /locus_tag="VIN32_04920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921954.1"
                     /translation="MTRTVRKALALIAIGSLMAACSTPAGSTGTTVTATLKEWEIKLS
                     SADLKAGDITINFTNNGDKEHEFLVRKTDLKADSLPIVASGDDKGRVDEASSQLTEVG
                     NPSEIAVLPPGTTGLSLRLKLQPGHYVVFCNLHVGDLLHYQKGMHTEFTVT"
     gene            3817..4920
                     /locus_tag="VIN32_04925"
     CDS             3817..4920
                     /locus_tag="VIN32_04925"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEY8921955.1"
                     /translation="MAIPPYTTPIVTDATPYAGQPATNAGPIGVSTGTRAERWASWLH
                     ITRQVAQGVLDAAAIATIALTFTSTVLPADVMFHIVFVLLIVQAFLFGLRGTVLRIGI
                     VSIPLLVYANAQSFGLTEPKFDLAEWPLMFVIALLVAWMADRVDTTAKRYASLFRQAS
                     ERLLTVEEDERRRIALELHDGVGQVLTAMTLTLDAAGGSLGPRARAQRVTAARRMAAT
                     ALAEVRHLAHRMRPARLEERGLTAAIRDLAGQSGFPVRVHVDPQLDPMPALEPTMTVG
                     VYRIVQEALANAARHSGAPMAHVSIALRDRRLLVLVSDEGRGFNPRTAIDPGIGLAGM
                     YERAELLGGSLSIQSAEGRGTSVALDLPLRATD"
     gene            4920..5561
                     /locus_tag="VIN32_04930"
     CDS             4920..5561
                     /locus_tag="VIN32_04930"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY8921956.1"
                     /translation="MEKIRVGIVDDHALVREGLRLILEREPDIEVVGEAADATAAIAL
                     AAGRKPQVMVVDLTLGESDGVALLHDLTGRYPHIRLVTVTMHEHEETVRQAFLAGAAG
                     YVVKGAPSVDLIAAIRAVARNQHYVHPMVASVVVVDSLRWLRQADRLTPREIEVLRLL
                     TAGRTAVETGRALGISAHTVRRHIANVTGKAGVRGRVALTRYALENHLVLDET"
     gene            5607..>6185
                     /locus_tag="VIN32_04935"
     CDS             5607..>6185
                     /locus_tag="VIN32_04935"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEY8921957.1"
                     /translation="MQLETPPRPSRRLAGRRLVVLPLMALAAIALISLATRPLGGEPQ
                     PSFFVIGSAVPGVQIGQAAPGTAQAPASPQLALKDLEGHPIALRDFGGRPLWIIFWKT
                     ACDACEAEAADVAAAYAAHRGAGLVLLGIDVWDTPAVVHDYTQAHAVDYPIAVDTSTS
                     FIDAYGVWGAPTHYFIGSDGVIRDRYFGPMNSE"
BASE COUNT         1138 a         2022 c         2040 g          985 t
ORIGIN      
        1 aggacgtaga gggtcaaaaa gaggaagacc cccgcggcaa cgacgaagtg ccagtagtag
       61 ctcgtcgcct ccacaatggt gtggtgctcg gccgagacga gtccacggcg cctgcgcgtg
      121 gcgacaatcg ccatcagccc aaggccagcc aggacatggg ccgcatgaaa gccggtgagg
      181 acatagaagg tcgacccgaa ggtaccggcg tccaagccga aaccgtttgt cgcaaactca
      241 tagccttgaa tgacgatgaa gatcgtgccc agcaatagcg tcagacgggt ccagcgccgg
      301 atgcgcgatt ggtctccgct gcgagccgag cgcaccgtcc attgcatcgt tccgctcgat
      361 gccaccagaa gcagcatgtt gagccccggc aggaacaacg caggtcgctc gaggcccgcc
      421 accggcggcc acccggggct ctccgagcgg atcacgaagt aggcggcgaa gagggcaccg
      481 aagagcatca cctcggacag gatgccgagc ttgaccgcga gcagcatatt gccccgggcc
      541 gcatccggat cctgttcgcc taagcccatg tggtcgaggt gttcgtgacg atccgcagtc
      601 cccgcatgca cgctcatggt gttgatcgtc cctctgccgg caccccgatc agggggccgc
      661 ttccatactc gtatggcccc gtggtcacgg tgggaatgtg gtcgaagttc tggatcgggg
      721 gcggagacgc agtctgccac tccagggtct gggcgcgcca cgggttggaa gctgcctgcg
      781 ggccgcgccg ccacgaccgg acgatgttga agacgaagac tgccaccgac aggaagatgt
      841 tgaaggcggc aaagctgatc cacaggttga cgccgccgaa ctgcgccgga tagtcggcca
      901 cgcgccggcg cattccctcg atcccgagcc agaacatcgg gaggaaggtc gcattgaagg
      961 cgagcgtaaa ccaccagaag tggagcttcc ccagccgctc gctatacatc cggcccgtga
     1021 tcttggggaa ccagtagtag gtgcctgcga agaggccgaa gatcgctccc cccacgatcg
     1081 tgtaatggaa gtgggcgacc acgaaatagg tgtcgtggag ggtgagatcg gtcgggacgt
     1141 ctgcgaggaa gacgccggtg atcccgccta tcaggaacgc ccagatgaag ccgaagaccc
     1201 acagcatcgg caccttgaag tgaagccgcc ccatccagat cgtccccaag gcggagagga
     1261 agacgatccc ggttggcact gagatcagct cggtggtgag catgatcggg atggggaaga
     1321 acgtagccat gccgctcacg aacatgtgat gagcccagac catgaagctc atgacgccga
     1381 tgctgatgaa cgacaggacg gccagcgggt acgcaaaaag cggcttgcgc gcgaagaccg
     1441 gcacgatctc gaggatcgcg cccaagccgg gcaggcccat gatgtagacg gcggggtgtg
     1501 agtagaacca gaagaggtgc tggaacagga tcacgtctcc gccacgactg gccctgaaga
     1561 acgaggtgcc cagcacgcgg tcgaagagca gcatggtgaa cgcgcttgcc acgatcgagg
     1621 tcgccaccag cccaagcact gacgtcacgg tgagcgccca cacgaagata ggcatacgca
     1681 tcagcgacat gcccggcgcc cgcatccgga agaccgtgac caggaagttg atggagctca
     1741 ggatcgaggc gacgcccacc gtgacgaagg ccatcaggta cgcctgctgg ccaaggttgg
     1801 cctgctggct caggggcgga tatgcggtcc agccgctgtc gaagccgccc agcagcgggt
     1861 tgatggcgac caacaggatc gggagcggca ggagccagta gctgaaggcg ttgctcttgg
     1921 ggaacgccat gtcgcgcgcc ccgatcatga tcggcaccag gtagttcccc aggccaccga
     1981 cgatcgagat cagggcgacg gtgatcatca tcgccccgtg cgtgctgaag atctcgttgt
     2041 aggcttcctt tcccagcagc aggccgggct gtgccagctc gaggcgcatg agcatcgcga
     2101 aggtgcccgc gacgaggaac agggcgaacg ccgtcgctag atattggacg ccgatcacct
     2161 tgtggtcggt ggtgaaccgg aagtagcgcc gccaatcgcc ggccgtgccg tgggcggcat
     2221 gctcctcctg ggcgttcacc tcgcgccctg tggcccatat cagccagaag cggaaggcgc
     2281 cgatcccgag caggaagccg atcagcgcca ggaggtagcc gagcccgagg atctcgccgt
     2341 ccgtgatctc catcccgaga ctgcccgcca ggatcaacga ccccgcgccg atggccgcac
     2401 cgatcgccgc gccgagcacc gcacgcagga gcccgggctt caatgctctc acgacggctg
     2461 ctccgatcca ctggcaggcg gctgactccc tgcagggctg ggcgtcgatt gccgcagcca
     2521 ggcctcgagc gcagcccgat cgttcaccac cgtggtgaag cgcatgtccg tatggccgag
     2581 gccgcacagc tcagcgcaca ggccgggata cgtgcccgcc accgtcgcgg tgacgtagat
     2641 ctgcgtcgtg cgcccgggca cggcgtcctg cttgataccg aaggccggca cccagaagga
     2701 gtggatcacg tcatccgagt tgatgatgaa atggacccgc tccccgaccg ggatgtgcag
     2761 ctcctgcgaa tccacgcctg cagatgggta tgtgaaatgc caggcccact gctcggcgcg
     2821 aacctgaacc tcgaagtccg agacctgggc gccgcggatg tcgatcaggc cgaccgcgcc
     2881 gtagccgaag agacccacaa ccagcgcgaa gctgatgccg agccaaaccg tctggaaccc
     2941 ccgatggccg tggatcggcg gcccgtccgc ctcgtcccca tcggcccgga accagatcgc
     3001 tgagtaggcg agtcccacca ccacgaacat gagcaccggg accgacagga cggtgagcag
     3061 cacaaaggcc gagttttcga tgacggcctc gcgcgaggcg atgaccggca tgatctggat
     3121 gcccgaaacc aggacggccc cgacgatgga gaggaccgcc cagatcgcag cggcgatcca
     3181 gagatgtcgc cgtggactcg tcacgctctg ctagatccga tcaggtgacc gtgaactcgg
     3241 tgtgcatacc tttctggtag tgcaggaggt ctcccacgtg gaggttgcag aagacgacgt
     3301 agtggcccgg ttgaagcttc aatcgcaacg aaaggcccgt ggtgccgggc gggagcacgg
     3361 cgatctcgct gggattgcct acctccgtca gctgtgacga cgcctcgtcg accctgccct
     3421 tgtcatcgcc cgaggcgaca atcggcaggg agtctgcctt gagatccgtc ttgcgaacga
     3481 ggaactcatg ctccttgtcg ccgttgttgg tgaagttgat ggtgatatcg ccggccttga
     3541 ggtctgccga gctgagcttg atctcccact ccttcaaagt cgcggtgacg gtcgtgccgg
     3601 tcgatcctgc cggcgtcgag caggcggcca tgaggctgcc gatcgcaatg agcgccaacg
     3661 cctttcgcac cgttcgggtc atccttctcc tcctcgactt aagatgtgtc gcatggcaga
     3721 gccgcgcgac agtgccaggc gccgccgcag gcacctagca tcgctaccgg ttgcgcccgc
     3781 tcctatactc ggaaccagcc attctgggag tccgtattgg ccatcccgcc ctacaccacg
     3841 ccgatcgtca cagatgcgac tccgtacgcg ggccagccgg cgacaaacgc cgggccgatc
     3901 ggcgtgtcga caggcacacg cgctgagcgg tgggcctctt ggctgcacat cacccgccag
     3961 gtggcacagg gcgtcctcga cgccgcggcc atcgccacca ttgcgctgac ctttacctcc
     4021 accgtcctgc cggcagacgt gatgttccac atcgtctttg tgctcctgat tgtgcaggcc
     4081 ttcctgttcg gcctgcgcgg caccgtcctg cgaatcggca tcgtctcgat cccgctgttg
     4141 gtctacgcca acgcccagtc gttcggcctg actgagccga aattcgactt ggccgaatgg
     4201 ccgctgatgt tcgtgatcgc gttgctggtc gcctggatgg ccgatcgcgt agatacgact
     4261 gcgaagcgct atgccagcct ttttcggcag gccagcgagc ggctgctgac ggtggaggag
     4321 gatgagcgcc ggcggatcgc cctggagctc cacgatggcg tcggccaggt gctgaccgcc
     4381 atgacgctca ccctcgacgc ggctggcggc tcgctgggac cgcgggccag ggcacaacgc
     4441 gtcacagcgg cgcgccgaat ggccgcgacg gccttggccg aggtgcgcca cctggcccac
     4501 cgcatgcgac cggcgcgcct cgaggagcgc gggctcactg cggcgatccg cgatctcgcc
     4561 ggtcagtccg gctttccggt ccgcgtccac gtggacccgc agctcgatcc catgccggcc
     4621 ctggagccga cgatgacagt gggggtctat cgaatcgtcc aagaggcgtt ggccaatgcg
     4681 gcgcgccaca gcggagcgcc gatggctcat gtgtcgatcg ccctaaggga ccggcgcctg
     4741 ctggtcctgg tctccgacga gggtcgcggc ttcaatccgc gcacggccat cgatccgggg
     4801 atcggcctgg ccggcatgta cgagcgggcc gagctgctgg gcggaagcct ctcgatccag
     4861 tcggccgaag gccggggcac cagtgttgcc ctggatctgc cgctccgggc taccgactga
     4921 tggagaagat ccgcgtcggc atcgtcgacg atcacgccct ggtccgcgag gggctccggc
     4981 tgatcctcga gcgagagccg gatatcgagg tcgtcggaga agcagcagac gcgacagctg
     5041 ccatcgccct cgcggcggga cgcaagccgc aggtgatggt ggtcgacctg accctgggcg
     5101 aatccgatgg cgttgcgctg ctgcatgacc tcaccggccg ctatccccat atccggctgg
     5161 tgacggtcac gatgcacgag cacgaggaga ccgtgcgcca ggcctttctg gccggagccg
     5221 ccgggtacgt cgtcaagggc gcgccgagcg tggacctgat cgcggcaatc cgcgccgtcg
     5281 ccaggaacca gcactacgtc cacccgatgg tggcctcggt ggtggtcgtc gactccctgc
     5341 gatggctccg ccaggcggat cggctcaccc cgcgcgagat cgaagtcctg cgacttctca
     5401 ctgcggggcg aacggctgtc gagacggggc gtgcgctcgg catcagcgcg cataccgtgc
     5461 gccgccacat cgcgaacgtg acaggcaaag cgggcgtccg cgggcgggta gcgctgaccc
     5521 gttacgccct tgagaaccac ctcgtgctgg acgagactta gccgctctcc gggacgtcga
     5581 ccgcggctcc gatagaatgc ggccccatgc agctcgagac gccgccccgc ccctcgcgga
     5641 ggctggccgg gcggcggttg gtcgtcctgc cactcatggc gctggcagcc atcgccctca
     5701 tctccctcgc cactcgtccg ctcggtggtg agccgcagcc atcgttcttc gtgatcggca
     5761 gcgcggtgcc gggggtgcag atcggacagg cggccccggg caccgcccag gcacctgcat
     5821 ccccgcagct tgcgctgaag gacctcgagg gacatccgat tgcgttacgc gacttcgggg
     5881 gtaggcctct ctggatcatc ttctggaaga ccgcctgcga tgcgtgcgag gcagaggcgg
     5941 ccgatgtcgc cgccgcctac gcggcgcacc gtggggccgg actcgtgctt ctgggaatcg
     6001 acgtgtggga tacgccagcc gtcgtccacg attacaccca ggcgcatgca gtcgactatc
     6061 cgatcgccgt cgacacctcg acaagcttca ttgatgccta cggcgtgtgg ggggcgccga
     6121 cgcactactt catcggttcg gacggcgtca tccgggatcg ctatttcgga ccgatgaaca
     6181 gcgaa
//
LOCUS       DATCQR010000148         1961 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_8403, whole genome shotgun sequence.
ACCESSION   DATCQR010000148 DATCQR010000000
VERSION     DATCQR010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1961
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8403"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            179..1807
                     /locus_tag="VIN32_04940"
     CDS             179..1807
                     /locus_tag="VIN32_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921958.1"
                     /translation="MAGVVNINEVLDGHVSLQVDCVDRLYLNAYCPILQVGGQVVGFL
                     TGHLGNPVPSPALFHQIGNRFRREVDRFAAGAGVPLLRLKKPDRSRWDDRKLDHVRPY
                     LDRAAAEGRFGVVAIVVAQEFQWVIGARNRSPKPGVVSYDFVKEERRVSAYYFYVHDR
                     EFGPGFIKICSYFPYPAKVWVNGHEWAKRQAEHEGLSYAALANGFAACLEPGRLQAIC
                     DRFGPADAQAFFDRWTAVIPVPFTAADRAAGYWWQLSMRQVEVSRTLVFNDPRRARSF
                     FESLVTDNIGIGRPEQIAAVFAAQVRTRGPRRTKGPFRTRVFSPGTEVKIDFSFRHSR
                     VKQYLKEGRALRIETVINKPADIGVLARLEHLPELVGKARQINDRLLMIERAGQGCAI
                     GSALFERIHQPYIGEGQRTGALRFGDQRAMALAGSICLVVHAATGLTNKSLRGHVAGL
                     LGRDYSSSQMSYDLRRLRLHGLIERTPGTNSYTLTPQGIRVAVFYTKLHARLLRPLLE
                     AHQPPAPIELRRALATIERVLGDYVTNARLGAAA"
BASE COUNT          327 a          699 c          618 g          317 t
ORIGIN      
        1 ggcccgaggg ctgtccgctg ttaaccctgg accgtgactt gcgacatgat cgtgaacact
       61 ctaggaaaga ccattagcgg gaggggggca ggcccccctc ccggccgccc ccccgtgtcg
      121 gctgggcctg gtgaactgcg ggccggcaaa gccggccctc gtcgtgcacg ctggttgcat
      181 ggctggtgtg gtgaacatca acgaggtgct ggacggacat gtcagcttgc aggtcgactg
      241 cgtggatcgg ctgtacctca acgcctactg cccgatcttg caggtcggcg gtcaggtggt
      301 tggtttcctg accgggcacc tgggcaaccc ggtcccctct ccggcactgt tccatcagat
      361 cggcaaccgg ttccggcggg aggtcgatcg cttcgccgcg ggagcgggtg ttccgctgct
      421 gcgcctgaag aagccggacc gctcccgctg ggatgaccgc aagctggatc acgtgcgccc
      481 ctacctggac cgggcggccg ctgagggacg cttcggggtg gtcgccatcg tggtggccca
      541 ggagttccag tgggtgatcg gggcgcgtaa ccgctcgcct aagccggggg tggtcagcta
      601 cgacttcgtc aaggaagagc ggcgggtcag tgcctattac ttttacgtgc acgaccggga
      661 gttcggcccg ggcttcatca agatctgcag ttacttcccc tacccggcca aggtctgggt
      721 caacgggcac gagtgggcca agcggcaggc cgagcacgag ggcctgagct acgcggcgct
      781 ggccaacggg ttcgccgcct gtctcgagcc gggccggctg caggcgatct gcgaccgttt
      841 cggcccggcc gacgcgcagg ctttcttcga ccggtggacc gcggtgatcc cggtaccgtt
      901 cactgccgcc gaccgcgccg ccggctactg gtggcagctg tcgatgcgcc aggtcgaggt
      961 ctcccgcacg ctggtcttca acgacccccg ccgggcccgc agcttcttcg agtcgctggt
     1021 caccgacaac atcggcatcg gaaggcccga gcagatcgcc gcggtcttcg ccgcccaggt
     1081 gcggacccgc gggcctcgcc gcaccaaagg cccgttccgc accagggtgt tctcgcccgg
     1141 caccgaggtg aagatcgact tctccttcag gcacagccgg gtgaagcagt acctcaagga
     1201 agggagggcg cttcgcatcg agaccgtcat caacaagccc gctgacatcg gggtcctcgc
     1261 ccggctcgag cacctgcccg aactcgtcgg caaagcccgc cagatcaacg accgcctgct
     1321 tatgatcgag agagccggcc agggctgtgc catcggctct gcgctcttcg agcgcatcca
     1381 ccagccctac ataggggagg gccaacgaac cggagcactg cgcttcgggg atcaacgcgc
     1441 catggccctg gccggctcga tctgcctcgt cgtccacgcc gccaccggcc tcaccaacaa
     1501 aagccttcgc gggcatgtcg ccggactcct cggcagagac tacagctcct cgcagatgag
     1561 ctacgacctg cgccgcctcc gcctccacgg cctgatcgag cgcacgccgg gcaccaacag
     1621 ctacaccctc accccccagg gcatccgcgt cgccgtcttc tacaccaagc tccacgcacg
     1681 actactccgg ccgcttctgg aggcacacca gcccccggca cccatcgaac tacgccgcgc
     1741 actcgccacc atcgagcgcg tcctcggcga ctacgtcacc aacgcccgac tcggagccgc
     1801 cgcctgaaac ctgtcacaaa atccagagac cgatccacca agaagaccta gcgcgccgtc
     1861 gttcctggcc gagcgccgtc gactcgggcg tgtcgctggt aggcacggga catgcccgtc
     1921 gggcaaaata ggacgaggcc cctccatgta tgagctgtct c
//
LOCUS       DATCQR010000149         7144 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_178821, whole genome shotgun sequence.
ACCESSION   DATCQR010000149 DATCQR010000000
VERSION     DATCQR010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 7144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7144
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178821"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="VIN32_04945"
     CDS             <1..291
                     /locus_tag="VIN32_04945"
                     /inference="COORDINATES: protein motif:HMM:NF015669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HEY8921959.1"
                     /translation="GETASSAVAQATLRLSGDGTLSATTGCRAVTGRYTLSEGQVQVT
                     LDPYDTIACAAPLGDQDAHILGVLSNGFAASIDGDQLTLTAGDKGLSYRAEG"
     gene            complement(288..1004)
                     /locus_tag="VIN32_04950"
     CDS             complement(288..1004)
                     /locus_tag="VIN32_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8921960.1"
                     /translation="MEAATALLEGRNLRKTYRLSRRNQVKALRGVQIRIEPGEMVALM
                     GPSGSGKSTLMHILGLLHSPDEGDGPPAELSIAGVDVTSMSEGTRTRMRADTMGFVFQ
                     SYNLVPTLTAAENVALAGEYAGTGRREAMSAASEALGWVGLEERAGHRPMELSGGEQQ
                     RVAIARSLVNKPKLLLADEPTGNLDSGHSQEVLALLRRFNTERGQTLLLVTHDAEVGA
                     FCDRIIHMRDGLIQDGAASS"
     gene            complement(1004..2173)
                     /locus_tag="VIN32_04955"
     CDS             complement(1004..2173)
                     /locus_tag="VIN32_04955"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8921961.1"
                     /translation="MRLLRQLSRRKLRTTLTILGITIGIWALVVFSSMANKINSLVAG
                     GSEYFAGKILVSDASNLALGIGLAPMKIDVADQIRDLDGVAAAAAEVQMIFDPTANAF
                     GGAQTIVGTVAGSDNGHETFEIRAAQGRLPTAADEGSMVIALGSDMARQQSAGIGDTI
                     EVRGERFEVVGILEPTLTTPDTSVFVPLSAAQLLFHKILPAPVQDAIPADQLISQVVV
                     YPDPGTDPAALAALIKERVKNVKTLTGAEFDKQIGSATAIFNAIIIGVAVISLVVGGL
                     SVINTMAMSVAERTREIGIKRAIGGSRRRIIRELVTEAAVIGFIGGIIGLVLGAIVVV
                     FANEAGRSSGTILFDLTPGTAIFAVAFSTILGMVAGVIPAWSAARLDPVEALRYE"
     gene            complement(2237..2413)
                     /locus_tag="VIN32_04960"
     CDS             complement(2237..2413)
                     /locus_tag="VIN32_04960"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEY8921962.1"
                     /translation="MPRANRLPPSRLPELTALIDGGQLRVPPITAYPLEATPDALTAM
                     AAGHVRGKLVITIR"
     gene            complement(2426..2929)
                     /locus_tag="VIN32_04965"
     CDS             complement(2426..2929)
                     /locus_tag="VIN32_04965"
                     /inference="COORDINATES: protein motif:HMM:NF023144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase N-terminal
                     domain-containing protein"
                     /protein_id="HEY8921963.1"
                     /translation="MGEKAVGDMAVGDRAAAKMNARARDLDGEAEAWQRLHAVMYRIT
                     PEIAEVPGYFREGWTAKDAVAHLGTWMAEGAQMLRRIAAGTYREDELDVDAENERFLA
                     AMRDIPLDIVHLQAASAHAELRRAWAELPEVTPAAEFWLRKAGPDHLAEHLPRLEAWV
                     DEQEAEA"
     gene            complement(2963..3796)
                     /locus_tag="VIN32_04970"
     CDS             complement(2963..3796)
                     /locus_tag="VIN32_04970"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY8921964.1"
                     /translation="MEADEAERIFDEFAEAYRDWWGPIIAPAALQVLDDVEVPSGDPT
                     PFDLLDLGTGTGVLAVAALERWPGVRVVGVDPSSHMLELAAEAARRRSPALVPRFRTL
                     VASADRLPLADRSMDVATSSFVIQLVPSRARALREVLRVLRPGGRFACLTWQEDDLRF
                     APDEAFSDALDELQIIPPPDDRDVHPYTSASAAAAEFRRAGFREVNARDVWLEHRFTP
                     ESYLDLLEHWIDREVFSSLDEDSRRQLRAVALRMMAELRPDAFVWRRPLVRVVGRRPS
                     R"
     gene            complement(3817..4068)
                     /locus_tag="VIN32_04975"
     CDS             complement(3817..4068)
                     /locus_tag="VIN32_04975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921965.1"
                     /translation="MYTVEDETFVQARAIISALASELMGQHESGSSHPVDLDELVRTA
                     ADSLPPGNEAEYARGIADRLEKSFPINELFPRRRRARSS"
     gene            4151..5089
                     /locus_tag="VIN32_04980"
     CDS             4151..5089
                     /locus_tag="VIN32_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008921304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HEY8921966.1"
                     /translation="MDTAQPSVHIGRNQFHLAWDRSIEPIATVASGDVVEIDALDASG
                     GQITNDSTVADLANLDFSRVDQVNGPIAVEDAEPGDTLEIELLEFAPEDWGWTANIAG
                     FGLLADDFTDPALRITRLAGGMAEFLPGVHIPLAPFCGELGVAPPAEGAHSTIPPDVF
                     GGNMDTRHLTAGTKLYLPVFAPGGRFSLGDGHAAQGDGEVCGTAIETPMRARVRLSVR
                     KDLHVTAPEFLTPGPLARGTNIGPYYATDGVGADLMTAAREAVRRMIDYLGREHGLDA
                     IDAYLLCSVACDLKISEIVDAPNWIVTAYCPLSIFG"
     gene            complement(5113..6615)
                     /locus_tag="VIN32_04985"
     CDS             complement(5113..6615)
                     /locus_tag="VIN32_04985"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEY8921967.1"
                     /translation="MTATSVPGTAGEGLQRNAIGLTEVLFQSITHMAPAVAVALSIGA
                     ATGFAGKLTPLAVVFAMVACLFTAYSIGQLALHLPSAGGMYTYVARGLGPFFGWLMAW
                     AFALAEPLVAPILLAAFGLYGANFLTAYLHVGEGFTYLWVVLAIACGLLLWWLIYSGV
                     QLSTRTGVLLGAIEITIFVFISILLIVKAGSRNTVDVFIPGAEGVQPAFQGMIFCLLA
                     FIGFEAAAPLGEEAREPRRTIPKAVIWSTVGVGAFYVLCYYAATVYFGPDRMADFYTF
                     NNGDPWGFLGTDVLGTLGGILVVFAIINSSLANANSGATAATRSIFSMARGGLLPKFF
                     AAVHPKHRTPVNAIHAQVLVAIVVAVAGGLILADDPYPGSGPINIYVFIGTMLGLLFA
                     GMYMAVNVAVIGFYWRERRDEFNVIKHLLIPILGVLAMVPALLSVIGGLTIPLFDVEL
                     PAYTTSLRFTAPIVGAWMLIGIVVFVVLWFVNRAALDRMSEVYGGEGPGE"
     gene            complement(6672..>7144)
                     /locus_tag="VIN32_04990"
     CDS             complement(6672..>7144)
                     /locus_tag="VIN32_04990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921968.1"
                     /translation="PWGTLNDIALLVATLAIPPLMLAFWELGGLTPTPLAVVAQVSGW
                     IAVIVWCVTHAVFIAGALPFDYEQPAAGGLAVEWVALIVIGLWIAGANLLAGPWLNAI
                     RWFGMAAGLGFVMLPLGMLIGGVNHPLIYAGGVGYSIVFPVWAFLMGRHLTAIP"
BASE COUNT         1204 a         2444 c         2422 g         1074 t
ORIGIN      
        1 ggcgagaccg cctcgtcggc ggtcgcccag gcgaccctcc ggctgagcgg cgacggcacg
       61 ctctcggcga cgaccggctg ccgcgcggtc accggcaggt acaccctctc cgagggtcag
      121 gtgcaggtga cgctcgaccc gtacgacacg atcgcatgcg cagcgccgct cggcgaccag
      181 gacgcacata tcctgggggt gctctccaac ggcttcgcgg ccagcatcga cggagaccag
      241 ctgaccctga ccgcgggaga caaggggctc agctaccgcg ccgagggcta ggagctcgcg
      301 gcgccgtcct ggatcagccc gtcgcgcatg tggatgatgc ggtcgcagaa ggcgccgacc
      361 tcggcatcat gggtgacgag cagcagggtc tggccgcgct ccgtattgaa ccgccgcagg
      421 agcgcgagca cctcctggct gtgaccgctg tccaggttgc ctgtcggctc gtccgcgagc
      481 aggagcttcg gcttgttcac cagcgaacgc gcgatggcca cgcgctgctg ctccccgccg
      541 gagagctcca tcggccgatg gccggcacgt tcctccaacc ccacccagcc gagcgcctcc
      601 gaggccgcgc tcatcgcctc gcggcggccg gtgcctgcgt actcgccggc gagggccacg
      661 ttctcggccg cggtcagggt cgggacgagg ttgtaggact ggaagacgaa tcccatcgtg
      721 tcggcgcgca tccgcgtccg cgtcccctcc gacatggagg tcacgtccac cccggcgatg
      781 ctcagctcgg ccggcggccc gtccccttca tcgggcgaat gcagcaggcc caggatgtgc
      841 atcagggtgc tcttgcccga gccggaggga cccatcagcg caaccatctc gccgggttcg
      901 atcctgatct gcacgccgcg cagggccttc acctggttac gacggctgag ccgatacgtc
      961 ttgcgcaggt tgcgcccttc caggagggca gtcgcagcct ccatcattcg tagcgcagcg
     1021 cttcgaccgg gtcgaggcgt gctgcgctcc aggccgggat gactccggcg accatcccca
     1081 ggatggtcga gaaggccacc gcgaagatcg cggttccggg cgtgaggtcg aacaggatcg
     1141 tgcccgacga gcggccggcc tcgttggcga agacgaccac gatggctccc agcaccaggc
     1201 cgatgatgcc gccgatgaag ccgatcaccg ccgcctcagt caccagctcg cggatgatcc
     1261 gccgacgcga gccgccgatg gcgcgcttga tgccgatctc gcgcgttcgc tcagccaccg
     1321 acatggccat ggtgttgatg accgacaggc cgccgaccac gaggctgatg acggcaacgc
     1381 cgatgatgat cgcgttgaag atcgccgtcg ccgatccgat ctgcttgtcg aactcggccc
     1441 cggtcagggt cttgacgttc ttgactcgct ccttgatcag cgccgccaag gcggctggat
     1501 ccgttccggg gtccgggtag acgaccacct ggctgatcag ctggtcggcc gggatcgcat
     1561 cttgcaccgg ggccggcaag atcttgtgga acagcagctg ggcggcgctg agcggcacga
     1621 agaccgaggt atccggcgtg gtgagggtcg gctcaagtat gccaaccacc tcgaaccgct
     1681 cgcctcggac ctcgatggtg tcgccgatcc cagcggactg ctgccgcgcc atgtcgctgc
     1741 cgagggcgat caccatcgag ccctcgtccg cggccgtcgg gagacgcccc tgcgccgctc
     1801 ggatctcgaa cgtctcgtgc ccgttgtccg agcccgccac cgtgccaacg atggtctgcg
     1861 cgccaccaaa ggcattggcc gttgggtcga agatcatctg gacctcggcc gccgctgccg
     1921 ccacgccatc gaggtcgcga atctggtcgg caacgtcgat cttcatcggt gcgagaccga
     1981 ttcccagcgc caggttggag gcgtcggaca ccaggatctt gccggcgaag tactcgctgc
     2041 cgccggcgac cagggagttg atcttgtttg ccatcgagct gaagacgacg agcgcccaga
     2101 tcccgatcgt gatgcccagg atcgtgagcg tcgtccgcag cttgcggcgc gagagttggc
     2161 gcagcaggcg catcggtacc tcaataaggg gatccgccgt caggccggcg gagtggagaa
     2221 gcctactacg cggaggtcaa cggatcgtga tgaccagctt gccgcgaacg tggcccgccg
     2281 ccatcgccgt caacgcgtcg ggagtggcct cgagcgggta ggcggtgatg ggcggcacgc
     2341 gcaactggcc accgtcgatc agggcggtca gctcgggcag gcgcgacggg ggcagacggt
     2401 tcgcgcgagg caacggacgc cctggctagg cttccgcctc ctgctcgtca acccacgctt
     2461 cgagccgcgg caggtgctcg gcgaggtggt ctggcccggc cttgcggagc cagaactcag
     2521 cggcgggagt gacttcggga agctccgccc aggcgcggcg cagctccgcg tgggcagagg
     2581 ccgcctgcag gtgaacgatg tcaagcggga tgtcgcgcat ggcagccagg aagcgctcgt
     2641 tctccgcgtc gacgtccagc tcgtcctcgc ggtaagtgcc cgccgcgatc cggcggagca
     2701 tctgggcacc ctccgccatc caggttccga ggtgtgccac ggcgtccttg gccgtccagc
     2761 cctcgcggaa gtagccgggc acctccgcta tctccggcgt gatgcggtac atcaccgcat
     2821 ggaggcgctg ccaggcttct gcctcaccat cgaggtcccg cgccctcgcg ttcatcttgg
     2881 ctgcggccct gtcgcccact gccatgtcgc ccacggcctt ctcgcccacg aacggatgat
     2941 agtccgcggg tgcgaatcgg ccctagcggg atggccgccg gcccaccacc cgtaccagcg
     3001 gccggcgcca gacgaaggcg tcgggtcgaa gctccgccat catccgcagt gcgaccgccc
     3061 gcagctggcg ccgtgagtcc tcgtccaggc tggaaaagac ctcgcggtcg atccaatgct
     3121 cgaggaggtc caggtagctc tccggcgtga accgatgctc cagccagacg tcccgggcgt
     3181 tcacctcccg gaagccggcg cggcggaact ccgcggcggc cgctgaggcg gaggtgtagg
     3241 ggtgcacgtc gcgatcgtcc ggtggaggaa tgatctggag ctcgtcgagc gcatcggaga
     3301 aggcctcgtc tggtgcgaac cgcaggtcgt cttcctgcca ggtcaggcac gcgaagcggc
     3361 ctccgggccg cagcacgcgc agcacctcgc gcagcgctcg cgcccgactc ggaacgagct
     3421 ggatcacgaa ggacgaggtg gccacgtcca tgctccggtc agccagcggc aagcgatcgg
     3481 cgctggcgac cagggtccgg aaacggggca caagagccgg cgaccgccgc cgagccgcct
     3541 cggcggccag ctcgagcatg tgactcgacg ggtcgacgcc gaccacccga acgcccggcc
     3601 agcgctcgag cgccgcgacc gcgagtacgc cggtgccggt gccaaggtcc agcaggtcga
     3661 acggggttgg gtcgccgctc gggacctcga cgtcgtcgag aacctggagc gccgccgggg
     3721 cgatgatcgg gccccaccag tcgcgatacg cctcggcgaa ctcgtcgaag atgcgctccg
     3781 cctcgtcggc ttccatctgc gcgatctcta cctcggctac gagctgcggg cgcggcggcg
     3841 gcgcgggaac agctcgttga tcgggaatga tttctcgagc ctgtcggcga tgccgcgcgc
     3901 gtactcagct tcattgcctg gcgggaggct gtcagcggcc gtccggacca gctcgtccag
     3961 gtcgacgggg tggctggacc ccgactcgtg ctgccccatg agctccgagg cgagtgcgga
     4021 gatgatggcc ctggcctgca cgaacgtttc gtcttcgacg gtgtacatcg gtggctcctg
     4081 catgccgagc ttggtggatt ggcctgatcc tagcgtctgg gtcagttgac ccggtattaa
     4141 gatcgcggcc atggataccg cgcagccatc cgtgcacatc ggccgcaacc agttccactt
     4201 ggcctgggac cgctccatcg agccgatcgc gaccgtcgcc agcggcgacg tagtggagat
     4261 cgacgcactg gatgcgtcag gcggccagat cacgaacgat tcgacggtgg ccgacctggc
     4321 caacctcgac ttctctcgcg tcgaccaggt caacggcccg atcgcggtcg aggatgccga
     4381 gcctggggac acgctggaga tcgagctcct ggagttcgcc cccgaggact ggggctggac
     4441 ggccaacatc gccggcttcg ggctgctggc cgacgacttc accgatcccg ccctgcgcat
     4501 cacccgcctg gccggtggga tggccgagtt cctgcccggc gtccacatcc ccctcgctcc
     4561 gttctgtggc gagctgggag tcgctccacc tgccgagggc gcccactcga cgatcccgcc
     4621 cgatgtgttc ggcggcaaca tggacacccg ccacctgacg gcggggacga agctctacct
     4681 ccccgtgttc gcccccggcg gtcgcttctc gctgggcgac ggacacgccg cccaggggga
     4741 cggcgaggtg tgcggcacgg caatcgagac cccgatgcgg gcgcgggtgc gcctgtcggt
     4801 ccgcaaggac ctgcacgtca ccgcgccgga gttcctgacc cccggtccgc tggcgcgcgg
     4861 gaccaatatc ggtccgtact acgccaccga cggcgttggc gccgacctga tgaccgccgc
     4921 acgggaggcc gtgcggcgca tgatcgacta cctcggtcgc gagcatggcc tcgatgcgat
     4981 cgacgcctac ctgctgtgca gcgtcgcctg cgacctgaag atcagcgaga tcgtcgacgc
     5041 gccgaactgg atcgtgaccg cctactgccc cctgtcgatc ttcggctagt gccagaacgg
     5101 cgaggctaga ggctactcgc caggtccctc gcctccgtag acctcgctca tcctgtccag
     5161 cgccgcgcgg ttcacgaacc acagcaccac gaagacgacg atgccgatca gcatccaggc
     5221 cccgacgatc ggcgccgtga agcgcagcga agtggtgtac gccggcagct cgacgtcgaa
     5281 gagcgggatc gtcagcccac cgatgacgga gagcagggcg gggaccatgg ccagcacgcc
     5341 gaggatcggg atcagcaggt gcttgatcac gttgaactcg tcgcgccgct cgcgccagta
     5401 gaagccgatc acggccacat tcacggccat gtacatgccg gcgaagagca ggccgagcat
     5461 ggtgccgatg aagacgtaga tgttgatcgg gccgcttccc gggtatggat cgtcggccag
     5521 gatcaggccg ccggcaacgg ccaccacgat cgcgaccagc acctgggcgt ggatcgcgtt
     5581 caccggtgtg cggtgcttag ggtgcacggc cgcgaagaac ttcggcagga gcccgccacg
     5641 ggccatcgag aagatggatc gggtcgccgc ggtcgccccc gagttggcgt tggccagtga
     5701 gctgttgatg atcgcgaaga cgaccaggat gccgccgagc gtgccgagca catcggttcc
     5761 caggaagccc cacgggtctc cgttgttgaa ggtgtagaag tcagccattc gatcgggccc
     5821 gaagtagacg gtcgcggcgt agtagcacag gacgtagaac gcgccgaccc cgaccgtcga
     5881 ccagatcacc gccttgggga tggtgcgacg gggttcgcgc gcctcctccc ccaggggggc
     5941 cgccgcttcg aagccgatga aggcgagcag acagaagatc atcccctgga aggcaggctg
     6001 gaccccctct gcccccggga tgaacacgtc gacggtgttt cgcgagccgg ccttgacgat
     6061 cagcaggatc gagatgaaca cgaagatcgt gatctcgatc gctcccagca gcacgccggt
     6121 gcgagtcgag agctggacgc cgctgtagat gagccaccac aggaggagcc cgcaggcgat
     6181 cgcgagcacc acccacaggt aggtgaaccc ttcgcctacg tgcagatatg cggtgaggaa
     6241 gttggccccg tagaggccga aggccgccaa caggatcggc gcgaccaggg gctcagcaag
     6301 ggcaaaggcc caggccatca accagccgaa gaatgggcca agcccgcgcg caacataggt
     6361 gtacatgcca ccggccgacg gcaggtgcag ggcaagctgg ccgattgagt aggccgtgaa
     6421 caggcaggcc accatcgcaa acaccaccgc cagtggcgtg agctttccgg cgaatccggt
     6481 ggctgccccg atcgacaggg caaccgcaac cgccggcgcc atgtgggtga tcgactgaaa
     6541 gaggacctcc gtgaggccga tggcgttgcg ctggaggcct tccccagccg tgcccgggac
     6601 gctcgttgct gtcatgccgc ctctcctcct cgtgcgccgc tccgatgcgg cctccacatg
     6661 gtatggacct gtcaaggaat cgcggtgagg tgcctcccca tgaggaatgc ccagaccggg
     6721 aagacgatcg aatagccgac tcctcccgcg taaatgagcg ggtggttgac gccgccgatc
     6781 agcatgccga gtggcaacat cacgaagccc aggcccgcgg ccatgccgaa ccagcggatg
     6841 gcgttgagcc aggggccggc caacaggttg gcgcccgcga tccagaggcc aatgacgatc
     6901 agggccaccc actcgaccgc cagaccgccc gccgccggct gttcgtagtc aaacggcagc
     6961 gcacccgcga tgaacacggc gtgggtcacg caccacacga tcaccgcgat ccagcctgag
     7021 acctgtgcca ccacggcgag cggcgtgggg gtcaggccgc ccagctccca gaacgcgagc
     7081 atgaggggcg ggatcgcgag ggtcgcgacc agtagggcga tgtcgttcag ggttccccaa
     7141 ggct
//
LOCUS       DATCQR010000150         3266 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_117309, whole genome shotgun sequence.
ACCESSION   DATCQR010000150 DATCQR010000000
VERSION     DATCQR010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3266
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117309"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..580
                     /locus_tag="VIN32_04995"
     CDS             <1..580
                     /locus_tag="VIN32_04995"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEY8921969.1"
                     /translation="RLVVLPLMALAAIALISLATRPLGGGPQPSFFVIGSAVPGVQIG
                     QIAPGTAQAPASPPLSLIDLEGRPVELRDFAGQPVWIIFWKTACPACEAEAADVAAAY
                     AAHRPSGLVLLGIDVWDSPAVIQDYVAKHDLAYPIAVDTSTSFMDAYGVWGAPTHYFI
                     GSDGVIRDRYFGPMNSELIETSLKAILPATAP"
     gene            639..1076
                     /locus_tag="VIN32_05000"
     CDS             639..1076
                     /locus_tag="VIN32_05000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921970.1"
                     /translation="MPDHSATHRHRPRMPVAAIGVLVLVLAACSASSPTSEPPASQAA
                     VSQAPSVAASTAAGGTTVTLSGLAFSVAEITIPVGPLTFVSEDSVPHLLAEGQNGVEV
                     STPRVQKVSINPNSQGDLVFTVAGDYNITCTIHKTMNMVVHVQ"
     gene            1216..2277
                     /locus_tag="VIN32_05005"
     CDS             1216..2277
                     /locus_tag="VIN32_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012213352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HoxN/HupN/NixA family nickel/cobalt transporter"
                     /protein_id="HEY8921971.1"
                     /translation="MVDMALRLLRTPGPIRNRIVRMYAFLFVFNGVAWAVALLASISY
                     PILLPTAFLAYTFGLRHAVDADHIAAIDNTTRKLMQDGQRPVAVGLFFSLGHSTVVVA
                     LSVVIALFASVLTDIPALQEIGSLIGTSVSAAFLLLIGVINLIVLIDIYRMFRRVARG
                     GAYDEATLEEFLNERGLLARIFRPMLRAIGKSWHMYPLGFLFGLGFDTASEVALLGLA
                     ATSGANHVPIGFILVLPALFAAGMSLVDATDGVLMLGAYGWAYVKPIRKLYYNLNITF
                     VSVLIAFAIGGIEVMSILAERFGLHGGLWEFARNLDFGVIGFGIIAIFVASWTVSTLI
                     YRWKRYDSLPIRSATGTDA"
     gene            2322..2720
                     /locus_tag="VIN32_05010"
     CDS             2322..2720
                     /locus_tag="VIN32_05010"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY8921972.1"
                     /translation="MMDPAPHAALSNGPSPRAPHRSRAERRAAALRSEGAATAALNVL
                     GQKWVMRIVRVLGERTQRFCELQDALGGANSATLSQRLKLLEDEGLVDRTIVSEVPPW
                     VEYSLTAKGSDLRRAIVGIDRWADRWAPNQ"
     gene            2717..3214
                     /locus_tag="VIN32_05015"
     CDS             2717..3214
                     /locus_tag="VIN32_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012876293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEY8921973.1"
                     /translation="MSHPVAEERPVEHGDALNLIRTAIAEAPGTEAAAQRAVELLHDR
                     FAHYDWVGIYWLDGSGTELVLGPWIGPEATEHTRIPVGVGICGAAAASGQTVIVDDVD
                     ADPRYLACFSTTRSEIVVPIFADGQVAGEIDIDGSDLAAYDQTDANFLEEIAALLAPL
                     RPRDA"
BASE COUNT          514 a         1162 c         1039 g          551 t
ORIGIN      
        1 gcggttggtc gtcctgccac tcatggcgct ggcagccatc gccctcatct cccttgcaac
       61 ccgtccgctc ggcggcgggc cgcagccgtc gttcttcgtg atcggcagcg ctgtgccagg
      121 cgtgcagatc ggccaaatcg cgccgggtac ggcccaggcg cccgcgtcgc cgccgctttc
      181 cctgatcgat ctcgagggta ggccggtgga acttcgggac ttcgctggcc aaccggtgtg
      241 gatcatcttc tggaagacgg cctgcccagc atgcgaggcg gaagccgcag acgtcgccgc
      301 cgcctacgcg gcgcaccgtc cgtcgggcct cgtcctcctg gggatcgacg tatgggacag
      361 ccctgccgtc atccaggact acgtcgccaa gcacgacctc gcctacccca tcgccgtcga
      421 cacctcgaca agcttcatgg atgcctacgg cgtgtggggg gcgccgacgc actacttcat
      481 cggttcggac ggcgtcatcc gggatcgcta tttcggaccg atgaacagcg aattgatcga
      541 gacgagcctg aaagcgatcc tccctgcgac tgcaccatga ggaactgcaa cccagctcgt
      601 atgaacaggc agatcacaag ccagcaggag tagaccccat gccagatcac tcggcgaccc
      661 atcgtcatcg gccgcggatg cccgtggcgg caatcggcgt cctcgtcctg gtgctcgctg
      721 catgcagcgc gagcagtccc accagcgagc cccccgccag ccaggcagcg gtcagccagg
      781 cgccgagcgt cgcggccagc acggcagccg gcggcacgac cgtcacgctg agcgggctgg
      841 ccttctcggt cgccgagatc accattccgg tcgggccact gaccttcgtc agtgaggaca
      901 gcgtgcccca cctccttgcc gagggccaga acggggtgga ggtctccacc ccccgggtcc
      961 agaaggtgtc gatcaacccg aactcgcagg gagatctggt cttcacagtc gcgggcgact
     1021 acaacatcac ctgcacgatc cacaagacga tgaacatggt ggtccacgtt cagtagcgat
     1081 cccgcgtcgt cgcgaagggc cggtgcatgc agcgccggcc cttttcgttg gccgcctcgg
     1141 ctttcgaatc atgtcgctaa tgcaaccggt tgcagtaagc ctttgagcct gcctagaatc
     1201 gacccggagg aaccgatggt cgacatggcg ctccgtctcc tgcgaacccc cggcccgatc
     1261 cggaaccgca tcgtgcgaat gtatgcgttc ctcttcgtct tcaacggcgt ggcctgggct
     1321 gtggcgctgc tggcgtcgat ctcctacccg atcctcctcc ccacggcgtt cctggcgtac
     1381 accttcggcc tccgccatgc ggtcgatgca gaccacatcg ccgccatcga caacacgacc
     1441 cgcaagctga tgcaggacgg ccagcggccc gtcgcggtcg gcctcttctt ctcgctcgga
     1501 cactcgaccg tggtggtcgc cctgtcggtc gtgatcgcgc tcttcgcatc ggtgctcacc
     1561 gacatccccg ccctacagga gatcggcagc ctgatcggca cgagcgtgtc ggcggcattc
     1621 ctgctgctca tcggcgtcat caacctgatc gtcctgatcg acatctaccg catgttccgg
     1681 cgcgtggcgc ggggcggcgc ctacgacgag gccaccctcg aggaattcct caacgagcga
     1741 ggcctgctgg cccggatctt ccggccgatg ctgcgggcga tcggcaagag ctggcacatg
     1801 tacccgctgg gcttcctctt cggcctcggg tttgacaccg cgtccgaggt ggcgctgctg
     1861 ggcctggcgg caacctcagg cgccaaccac gtgccgatcg gcttcatcct ggtcctgccc
     1921 gccctcttcg cggccggcat gtcgctggtc gatgcgacgg acggggtcct gatgctgggc
     1981 gcctacggct gggcgtacgt caagccgatc cgcaagctct actacaacct gaacatcacc
     2041 ttcgtgtcgg tgctgatcgc cttcgccatc ggcgggatcg aggtcatgtc gatcctcgcg
     2101 gagcggttcg ggctccacgg tggcctctgg gaattcgcgc gcaaccttga cttcggggtc
     2161 atcggcttcg ggatcatcgc catcttcgtc gccagctgga ccgtgagcac gctcatatac
     2221 cgctggaagc gatacgactc gctccccatc cggagcgcga cggggaccga cgcctagcgc
     2281 gattgcgcgt gccggagggc ggtgctatcg tccgccgcct gatgatggac cccgccccgc
     2341 acgctgcgct gtcgaacggg ccgtcgcctc gggcgccgca ccgctcacga gcggagcgac
     2401 gggcagccgc cctgcgcagc gagggggcgg cgaccgccgc cctcaacgtg ctcggccaga
     2461 agtgggtgat gcgcatcgtg cgggtcctcg gggagcgcac gcagcgcttc tgcgagctgc
     2521 aggacgccct tggtggggcc aactcggcga ccctctcgca gcgcctcaag ctgctcgagg
     2581 acgagggact ggtcgaccgc accatagtct ccgaggtccc gccgtgggtg gaatactcgc
     2641 tcaccgccaa gggatcggac ctgcgtcgcg caatcgtggg gattgaccgc tgggcggacc
     2701 ggtgggcgcc caaccagtga gccaccccgt cgcagaggag cgacccgtcg agcatggcga
     2761 cgccctcaac ctgatccgca ccgccatcgc cgaggccccg ggcaccgagg ccgccgcgca
     2821 gcgcgccgtg gagctgcttc acgaccgctt cgcgcattac gactgggtcg gcatctactg
     2881 gctcgacggc agcggcaccg agctcgtgct cggcccgtgg atcggtccgg aggccaccga
     2941 gcacacccgg atcccggtcg gtgtcgggat ctgcggggcc gccgccgcat cgggccagac
     3001 cgtgatcgtc gacgacgtcg acgccgatcc gcgctacctc gcctgcttca gcaccacgcg
     3061 cagcgagatc gtggtcccaa tcttcgccga cggacaggtg gccggcgaga tcgatatcga
     3121 cggctcggac ctggccgcct acgaccagac cgatgcgaac ttcctggagg agatcgccgc
     3181 gctcctggct ccccttcgac cgagggacgc ctagccgcgt aacccgcggc gtatcggaga
     3241 tgaatggccg attctgaacg cgtcac
//
LOCUS       DATCQR010000151         6123 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_224555, whole genome shotgun sequence.
ACCESSION   DATCQR010000151 DATCQR010000000
VERSION     DATCQR010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6123
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224555"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            47..1519
                     /locus_tag="VIN32_05020"
     CDS             47..1519
                     /locus_tag="VIN32_05020"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY8921974.1"
                     /translation="MTTETSAQPAIGNVKMIIGGEQVDAADGQTFDVMNPATGEVLAR
                     APLGGAEDVDRAVKAAQAAFEGPWSTWSASKRGRTLQKFSSLVKQNLEELAQLESRNV
                     GKPISGARGEALAVSLVFEYYAGAANKLFGETIPVSRPGLDFTLREPIGVVGLIVPWN
                     FPMNMASWKLGPALAAGNTAILKPASYTPLTAIRLGELALEAGFPPGVVNVVTGPGGT
                     AGAALAGHPGVGKVAFTGETTTGQEIMRLAANNVTKVSLELGGKSPNIVFADADLERF
                     AAESPLAVFDNAGQDCCARSRIFVEASVHDKVVELFTEATRKVVVGDPSEDATQMGSL
                     ISERQRERVVDYIGIGREEGAELVYGGEAPTGDPFDIGAYLMPTIFDAASTDMRIVRE
                     EIFGPVVAIIPFSDEAEAIRLANDTPYGLSGSIWSHDIGRALRVARAVKAGVLSVNTN
                     SSVHTEAPFGGYKMSGMGRELGMHALELYTEVKNVFVDLT"
     gene            complement(1491..2240)
                     /locus_tag="VIN32_05025"
     CDS             complement(1491..2240)
                     /locus_tag="VIN32_05025"
                     /inference="COORDINATES: protein motif:HMM:NF019342.4"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEY8921975.1"
                     /translation="MRDRPRIVVTLHGPEQAASEEPTWASFDAYLDAVRRAGGDPVAL
                     DPTAGDADRATALATMDGLLLPGGADLDPALYGEEPHPAVAVEGSRDDLELAAWTAAR
                     ERGVPIFGICRGFQAINVFSGGTLLQHLEGHDSPTQAPEAHPLHLDPASRLASILGET
                     DPLLSTQVNSYHHQAVRPADLARGLVASALAPHDEGELVEGLEAGDPDDWLVGVQFHP
                     ERPEFIGPEFDRLWRAFVDAARIRSGRRRRS"
     gene            2279..2797
                     /locus_tag="VIN32_05030"
     CDS             2279..2797
                     /locus_tag="VIN32_05030"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX pyrophosphatase"
                     /protein_id="HEY8921976.1"
                     /translation="MTESKMRAEGWPAHRVTGVDVWPYRLFEGTAEFLLLHRSGRDGA
                     PFWQGVSGWIEAGEAPHLAALRELREETGLEAVELYSVDAVFDLYAWRRGTVEAIVPF
                     AALIADDAGPQLSEEHDEWRWEPLAEAVELVPYEPQRSALQRISADLLERPDLAHLYR
                     IGPPFEGHEKSR"
     gene            2951..4579
                     /locus_tag="VIN32_05035"
     CDS             2951..4579
                     /locus_tag="VIN32_05035"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEY8921977.1"
                     /translation="MDSSQGDQIARDDAHLRSLGIKPELRRTLGFLSNFAVAFSYISV
                     STGTFTNQAVAFGVGGPAIFWAWPLVILGQTFVALNFAELASHFPVAGSIYQWSKRLS
                     NKTLGWFTGWIYFWAGVVTVTAVAATVPLVLSTIMGFDLASDSPIPGLDMWQFVGLAA
                     LVTTTLINIEGVRLLALINNVGVGAEILGMVVFALILLFFANHQSPAILFDTSFTANL
                     SDGGYLPIFLVGMFMALFVVYGFDTAGTFGEETVDAGRQAPRGVLSAIWLSGLVGGIF
                     LLAVTLSFQDVGAAVATGQAFGFPIADTIKENLTFSFGFITLGELYLVMILIAVYVCT
                     LAIQGATVRLMFSMGRDRRLPFGAAWGHVNHTFRTPANAAVAVGILGAVPLLFMGALA
                     SIYLAIAATGMIYISYFLCNLGVFVARTKGWPHQGAWFSLRGWGTVINFVALLWGGVM
                     VINIGIWTSPIFGVFGNDLRNTWVNPFINTFLAFGKNADGTANVLTGLPAWPVFETLV
                     GLVFVVGLAYYLLVQRGREDRVEADLATGEAMIA"
     gene            4676..5620
                     /locus_tag="VIN32_05040"
     CDS             4676..5620
                     /locus_tag="VIN32_05040"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEY8921978.1"
                     /translation="MNEPEEELRGELRDQLVAALQRVPETAGRQLTLTALSGGITNRN
                     YLITAAGEPERYVIRLAGNDTHLLGISREVEHAATVAAAGVGIGPEVTAFIRPEGYLV
                     TRFIVGEPVSLEQIHQPATIRRVADSIRRIHGGPAIPGLFVPLRIVEAYLALAMERGV
                     PRPAAWEQAHEVGRRIERALLEARIDLRPCHNDLLNANFIDDGERIRIVDWEYAGMGD
                     PFFDLGNFSVNHDLTPDEDAILLEAYEGEVRPSRLARLALMRIVSDFREAMWGVLQQG
                     ISTLDVDFVEYADEHFERLLTNGRGLGFEVQLHEATRG"
     gene            5613..>6123
                     /locus_tag="VIN32_05045"
     CDS             5613..>6123
                     /locus_tag="VIN32_05045"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018225532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY8921979.1"
                     /translation="MADRDGGLPERARCVIIGGGVGGAAIAYHLTLLGWTDVVLLDRS
                     ELTSGSTFHSAGLVGQLRSSPTLTRMMVDSVDTYRGLATETGVDPGWREVGSLRLASS
                     AERMEELRRQAGWAKAYGLPMELVSAEEAQRLFPLMHTDGVLGAAWLPTDGYLDPSGL
                     TQALAAGARS"
BASE COUNT          940 a         2085 c         2104 g          994 t
ORIGIN      
        1 gtacccgcgc agccatcgcc ctcgccctga tgcctagaat tgcgccatga ccaccgaaac
       61 cagcgcccag ccggcaatcg gcaacgtgaa gatgatcatc ggcggcgagc aggtcgatgc
      121 cgctgacggg cagaccttcg acgtcatgaa cccggcgacg ggtgaggtcc tggcccgcgc
      181 gccactcggc ggcgccgagg acgtcgatcg cgccgtcaag gccgcacagg ccgccttcga
      241 gggtccctgg tcgacctggt ccgcctcgaa gcgcgggcgg acgctgcaga agttctccag
      301 cctcgtcaag caaaacctcg aggagctcgc ccagctcgag agcaggaacg tcggaaagcc
      361 gatcagcggc gcccgcggcg aggcgctggc cgtcagcctc gtattcgagt actacgcagg
      421 tgccgccaac aagctcttcg gcgagacgat cccggtcagc aggccggggc tcgacttcac
      481 cctgcgcgag ccgatcgggg tggtcggcct gatcgtcccc tggaacttcc cgatgaacat
      541 ggccagctgg aagcttggcc cggccctggc ggcaggcaac accgcgatcc tcaagccagc
      601 cagctacacc ccgctgaccg ccatccgcct cggcgagctg gcgctcgagg cggggttccc
      661 gccgggcgtc gtcaatgtcg ttaccggtcc cggcggcacg gccggcgcag ccctggcggg
      721 ccatccagga gttggcaagg tggccttcac cggcgagacg acgacggggc aggagatcat
      781 gcggctggcc gccaacaacg tgacgaaggt ctcgctcgag ttgggtggca agagccccaa
      841 catcgtcttc gccgacgcgg acctggagcg cttcgccgcc gagtcgcccc tggccgtctt
      901 cgacaacgcg ggccaggact gttgcgcgcg cagccggatc ttcgtggagg catcggtcca
      961 cgacaaggtc gtcgagctgt tcaccgaggc gacccgcaag gtggtcgtcg gcgacccgtc
     1021 ggaggacgcg acccagatgg gcagcctcat cagcgagcgg cagcgcgagc gcgtggtcga
     1081 ctacatcggg atcgggcgcg aggagggcgc ggagctggtc tacggcggcg aggcgccgac
     1141 cggcgacccg ttcgacatcg gcgcctacct gatgcccacc atcttcgacg ccgccagcac
     1201 ggacatgcgc atcgtgcgcg aggagatctt cggcccggtg gtggcgatca tcccgttcag
     1261 cgacgaggcg gaggcgatcc ggctggcaaa cgacacaccg tacgggctct ccggctcgat
     1321 ctggagccac gacatcgggc gggccctgcg ggtcgccagg gcggtcaagg ccggggtgct
     1381 atcggtcaac accaacagca gcgtccacac cgaggcgccg ttcggcggct acaagatgag
     1441 cggcatggga cgcgagctcg ggatgcatgc gcttgagctg tacaccgagg tcaagaacgt
     1501 cttcgtcgac ctgacctgat ccgcgcggcg tccacgaagg cacgccacag gcggtcgaac
     1561 tcggggccga tgaactcggg gcgctccggg tggaactgga cccccaccag ccagtcatcg
     1621 ggatcgcctg cctccagccc ctcgacaagc tcgccttcgt catggggcgc gagcgctgag
     1681 gcgaccagcc cccgagcgag gtcagcgggt cgaaccgcct ggtggtggta gctgttcacc
     1741 tgcgtgctga ggagcggatc cgtctcgccc aggatcgagg cgaggcgcga ggccggatcg
     1801 agatgcagcg ggtgcgcctc aggcgcctgg gtcggactgt cgtggccctc gaggtgctgc
     1861 agcagcgtcc cgcccgagaa gacgttgatc gcctggaagc cgcggcagat cccgaagatc
     1921 ggcacgccgc gctcgcgcgc ggccgtccat gccgccagtt cgaggtcgtc acgcgacccc
     1981 tccacagcca ccgcagggtg cggctcctcg ccatacaggg cggggtcgag atccgcaccg
     2041 cccggcagga gcagcccgtc catcgtggcc agggcggtcg cgcggtccgc atcgcccgca
     2101 gttgggtcga gagcaaccgg atccccgcca gcgcggcgca cggcgtcgag ataggcgtcg
     2161 aacgaggccc aggtcggctc ctcgctcgct gcctgctccg ggccgtgcag ggtgacgacg
     2221 atccggggac gatccctcat agtccgatga tacgggcgcg cctcctacaa tctgccgcgt
     2281 gaccgagagc aagatgcgcg ccgagggctg gcccgcgcac cgcgtgacgg gggtcgacgt
     2341 gtggccctac cgcctgttcg agggcacggc cgagttcctg ctgctgcatc ggtccggccg
     2401 tgatggcgcc cccttctggc agggagtttc cggctggatc gaggcgggcg aggccccgca
     2461 cctcgccgcg ttgcgggagc tgcgcgagga gaccgggctc gaagccgtgg agctgtactc
     2521 ggtggatgcg gtcttcgacc tgtacgcctg gcgtcggggg acggtcgagg cgatcgtccc
     2581 gttcgcggcg ctgatcgcgg acgacgcagg gccgcagctc tccgaggaac acgacgagtg
     2641 gcgctgggaa ccgctcgcgg aggcggtcga gctcgtcccg tacgagcccc agcgatcggc
     2701 gttgcagcgg atctcggccg acctcctcga gcgcccggac ctggcgcacc tgtaccgcat
     2761 cggcccccca ttcgagggcc acgaaaaaag tcgttgactc cccatctggg cactcctatg
     2821 ctgccgccca gcccggccga tcggtcgtcc aaattgctgc atcaccgatc cgcgcggata
     2881 ccgtcggtcg tttcgcgacg gaggctcacg cccgaccagg tgtgatggag aaggaggacc
     2941 tgtcttgacc atggattcca gtcagggcga ccagatcgcg cgcgacgatg cgcacctgcg
     3001 atcactcggc atcaagcctg agctgcggcg aacactcggg tttctgtcga acttcgccgt
     3061 ggcattcagc tacatcagcg tgtcgaccgg cacgttcacg aaccaggcgg tggcattcgg
     3121 tgtgggaggg ccggccatct tctgggcttg gccgctggtc atcctggggc agacgttcgt
     3181 cgccctgaac ttcgcggagc tggcaagcca cttcccggtg gcggggtcga tctatcaatg
     3241 gtcgaagcgc ctctcgaaca agacgctcgg ttggttcacc ggctggatct acttctgggc
     3301 cggggtggtg accgtcacgg ccgtggctgc gaccgtcccg ctcgtgctgt ccacgatcat
     3361 gggcttcgac ctcgccagtg actcgccgat ccccggcctc gacatgtggc agttcgtagg
     3421 gctcgccgcg ctggtgacca cgacgctcat caacatcgaa ggagtccggc tgctggcgct
     3481 catcaacaac gtcggcgttg gcgcggagat cctggggatg gtcgtcttcg ctctcatcct
     3541 gctcttcttc gccaaccatc agtcgccggc catcctgttc gacacctcgt tcaccgccaa
     3601 cctgagcgac ggcggctacc tgccgatctt cctggtcggc atgttcatgg cgctcttcgt
     3661 ggtctatgga ttcgacacgg caggcacctt cggcgaggag acagtggacg ccggccgcca
     3721 ggcgccgcgc ggcgtgctgt cggccatctg gctctccggc cttgtcggag gcatcttcct
     3781 gctcgctgtg accctgtcct tccaggacgt cggcgccgcg gtggccactg gccaggcgtt
     3841 cggcttccca atcgccgaca cgatcaagga gaacctcacc ttctcgttcg gcttcattac
     3901 gctcggcgag ctctacctgg tgatgatcct gatcgcggtg tacgtgtgca ccctggcaat
     3961 ccagggcgcg accgtgcgcc tgatgttctc gatggggcgt gatcgccggc tcccgttcgg
     4021 cgccgcgtgg ggccatgtga accacacgtt ccggaccccc gccaatgccg ctgttgcagt
     4081 cgggatcctc ggcgccgtac ccctgctgtt catgggagcg ctggcctcca tctacctggc
     4141 gatcgcggcc acggggatga tctacatcag ctacttcctc tgcaacctcg gggtcttcgt
     4201 cgctcgtacc aagggctggc cgcaccaggg agcctggttc agcctgcgtg gctggggaac
     4261 ggtcatcaat ttcgtggcgc tgctgtgggg cggcgtgatg gtcatcaaca tcgggatctg
     4321 gaccagcccg atctttggtg tgtttggcaa cgaccttcga aacacctggg tcaacccgtt
     4381 catcaacacc ttcctggcct tcggcaagaa tgcggacggc acagccaatg tgctgacggg
     4441 gctgcctgcc tggccggtgt ttgagaccct ggtgggtctc gtcttcgtgg ttgggctggc
     4501 ctactacctg ctggtgcagc gcggccgcga agaccgcgtc gaggccgact tggccaccgg
     4561 ggaggcgatg atcgcctagc gtcccggatc cctcgcgagg tgagagcggc tcggcgccct
     4621 ggcgccgggc cgctcgctat cgtgtccggc agcacaggcc cggagagcgc ccaccgtgaa
     4681 cgaacctgag gaagagcttc gcggcgagct gcgtgaccag ctggtcgcgg cactgcagcg
     4741 cgtgccggag accgcaggcc gccagctgac gctgactgcc ttgtcggggg gcattaccaa
     4801 ccgcaactac ctgataacgg ctgccgggga gcccgagcgc tacgtgatcc gccttgcggg
     4861 caacgacacc cacctgctcg gcatcagccg cgaggtggag cacgccgcaa ccgtggcggc
     4921 ggccggggtg ggcatcggcc cggaggtcac cgccttcatc cggcccgagg gctacctcgt
     4981 cacccgcttc atcgtcggtg agccggtcag cctggagcag attcaccagc cggcgactat
     5041 tcgccgcgtg gccgactcga tccgccgcat ccatggcggg ccggccatcc cggggctgtt
     5101 cgttccgctg cgcatcgtgg aggcgtacct ggcgctggcg atggagcgcg gcgtgccgcg
     5161 gccggcagcc tgggagcagg cgcacgaggt tgggcggcgc atcgagcgcg ccctgctcga
     5221 agcgcggatc gacctgcggc cctgccacaa cgacctcctc aacgccaact tcatcgacga
     5281 tggcgagcgg atccggatcg tcgactggga gtacgccggc atgggcgacc ccttcttcga
     5341 cctcggcaac ttcagcgtca accacgacct cacgcccgac gaggacgcca tcctgctcga
     5401 ggcctacgag ggcgaggtgc ggccttcgcg cctcgcgcgc ctggcgctga tgcgcatcgt
     5461 gtccgacttc cgcgaggcga tgtggggcgt cctgcagcag gggatcagca cgctcgacgt
     5521 cgacttcgtt gaatacgcgg acgagcactt cgagcggctt ctcactaacg ggcgcggcct
     5581 cgggttcgag gtgcagctgc acgaggcgac gcgtggctga ccgcgacggg gggctgccgg
     5641 agcgagcccg ctgcgtgatc atcggtggtg gcgtcggagg cgcggcgatc gcctaccacc
     5701 tgaccctttt gggctggacc gatgtcgtgc tgctcgatcg gtccgagctg acatcaggct
     5761 cgaccttcca ctccgcgggg ctggtggggc agctccgctc gagcccgacc ctcacccgca
     5821 tgatggtcga cagcgtcgac acctaccggg ggctggcaac tgagaccggg gtcgatccgg
     5881 gctggcgcga ggtcggctcc ctgcggcttg cctcgtcggc ggagcgcatg gaggagctgc
     5941 gccggcaggc cggctgggcg aaggcctacg gactgccgat ggagctggtg agcgccgagg
     6001 aagcgcagcg cctcttcccg ctgatgcaca cggacggagt cctgggggcg gcgtggctcc
     6061 ccactgacgg ctacctcgac ccgagcggcc tgacccaggc gctggcggca ggcgctcgca
     6121 gcc
//
LOCUS       DATCQR010000152         3197 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_132394, whole genome shotgun sequence.
ACCESSION   DATCQR010000152 DATCQR010000000
VERSION     DATCQR010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3197
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132394"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            208..1173
                     /locus_tag="VIN32_05050"
     CDS             208..1173
                     /locus_tag="VIN32_05050"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY8921980.1"
                     /translation="MALSLVALVGYLAFIGYDGSQGFAAHERSGDCRTPASAFGWAYE
                     AINYDLASDQQLDAFPDRTECARPGEAAGADLTASDGIRIAGWYIPAGKSSPTAPTVV
                     LAHGNGESKSEMLRWAEPLHADYNLVLFDFRNHGQSSGDLTTLGVREVRDLKAIVDWL
                     EEAKSPPSIAVLGVSMGGVTAIDEAADDERVAAVILDSTHATLVGALQRQLESRGLPL
                     ALPGAWSILLGGLIRTGEDLSLADPVQAIERMGSRPMLIVEAGDDDAVGPTDAADLKR
                     AAPDKGAHVELKTCPKAKHGGSVETCPEYAGWVLGFLERSLPHGQ"
     gene            1245..1853
                     /locus_tag="VIN32_05055"
     CDS             1245..1853
                     /locus_tag="VIN32_05055"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011433087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY8921981.1"
                     /translation="MSLAPHDCPTSAPTASEATTLLADAQRAVVTTTLGTFTIELDAT
                     SAPIATANLVALARCDFYAGITFHRVIAGFVAQAGDPGTRTNRGSFDGLGTGGSGYGF
                     EVEFPDASQPYDRYTVAMANALQYDAISGEITSGRDTNGSQFFIDLASLPQLRPYYSV
                     LGKVTQGTDVIDTIGAVSVNNPQDGIPVDPVIIQSITIESGT"
     gene            2117..2192
                     /locus_tag="VIN32_05060"
     tRNA            2117..2192
                     /locus_tag="VIN32_05060"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2150..2152,aa:Arg,seq:ccg)
     gene            2231..>3197
                     /locus_tag="VIN32_05065"
     CDS             2231..>3197
                     /locus_tag="VIN32_05065"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEY8921982.1"
                     /translation="MPGLIRRDRRAREEEEERLSEPAARSTQSRGRVRPEEPDRFRTA
                     YERDRDRIIYSKAFRRLKHKTQVYLNPEGDHFVTRLTHTLQVAQIARALAAGLSLNDP
                     LAEAIALGHDVGHTPFGHTGEEALSPYFPPDGWHHAAQSVRVYEVLEDLNLTWEVRDG
                     IRAHSWKIEPPPSTQEALCVRYADRIAYLAHDALDAIRAGVLEADSFPPAAMARFGEP
                     GRVWIGEMIDAVLDNSLRAGEVRMDDETLDVMHELRAFMFERVYLAPSLLREQRAAID
                     TLGALVDHHLAHPDQIPATYREADAPLVTQVADYVAGMTDRYALAA"
BASE COUNT          529 a         1121 c         1047 g          500 t
ORIGIN      
        1 tgttcgggcg ccagcgagtg gccgcggtgc ctcggcacaa cgttcctggt ggcgggcctg
       61 gtgaccttcg gactaaccac gggccccgga ttgtagggga gccttccacg gggcaccacc
      121 cgtatactcg gccgagtgcc gttcgagccg ccgccagacc ctcgccaggc gcgccgtacc
      181 gcaatctggc ggtgggtctc gtccgcaatg gcgctttcgc ttgtcgccct ggtgggatac
      241 ctcgccttca tcggctacga cggctcgcag gggttcgcgg cccacgagcg ctcaggcgac
      301 tgccggacgc ccgcctccgc attcggctgg gcatacgaag cgatcaacta cgatctcgcc
      361 tccgaccagc agctggacgc gttcccggat cgcactgaat gcgcgcggcc cggtgaagct
      421 gcaggcgccg acctgaccgc aagcgacggg attcgcatcg cgggctggta catccccgcc
      481 gggaaatcga gtcccacggc gccgacggtt gtgctcgccc atggcaatgg ggagtccaag
      541 agcgagatgc tgcgctgggc cgagccgctg cacgccgact acaacctcgt cctcttcgac
      601 ttccgcaacc acgggcagag ctccggcgat ctcacgaccc tcggagtccg cgaggtccgc
      661 gacctcaagg cgatcgtcga ctggctggag gaggccaagt ccccgccctc gatcgcggtc
      721 ctgggggtct cgatgggtgg cgtaactgcc atcgacgagg ccgccgacga cgagcgagtt
      781 gctgcggtga tcctcgactc cacccacgcc accctggtcg gtgccctgca gaggcagctc
      841 gaaagcaggg gcctcccgct ggcattgccc ggcgcatggt cgatcctgct cggcgggctg
      901 atccgtacag gggaagacct cagcctcgct gaccccgtcc aggcgatcga gcggatgggc
      961 agtcgcccca tgttgatcgt agaggccgga gacgacgacg ccgtcggtcc gaccgatgct
     1021 gccgacctga aacgggccgc gcctgacaag ggggctcacg tcgagctgaa gacgtgcccc
     1081 aaggcgaagc acggcggatc ggtggagacc tgcccggagt acgccggctg ggtgctcggc
     1141 ttcctcgagc gctcactgcc ccacgggcaa tgagaggccg agcacggccc tgggctgggg
     1201 cgatcctggc actgggcctc gtggcatgcg gctcgccacc cctggtgagc ctcgcgccgc
     1261 atgactgccc gacctcggcg ccgaccgcgt ccgaggccac gacgctcctg gccgatgcgc
     1321 aacgggcggt ggtgacgacg acgctcggga ccttcacgat cgagctggat gccacatcgg
     1381 ctccgatcgc caccgccaac ctcgtggcgt tggcacgctg cgacttctat gccgggatca
     1441 ccttccatcg ggtcatcgcc ggcttcgtcg cccaggcagg cgaccccggc acaaggacga
     1501 accgcggctc gttcgatgga cttggcactg gtgggtcggg ctacggcttc gaggtcgagt
     1561 tcccggacgc gagccagccg tatgaccgct atacggtcgc catggcgaat gccctgcagt
     1621 acgacgccat cagcggcgag atcaccagcg gcagggacac caatgggagc cagttcttca
     1681 tcgacctcgc cagcctgccg cagctgcggc cctactacag cgtgctcggc aaggtgacgc
     1741 aggggaccga cgtgatcgac accatcggcg cggtgagcgt caacaacccg caggacggaa
     1801 tcccggtcga tccggtcatc atccagtcca tcacgatcga atcgggcacc tgaacggggt
     1861 gaatcggcct gaaaaccgct tcgaaatgta aacgattctt agcccccaac cattgaccca
     1921 tacccgacct caggcgtatc ctttcggggc tgttcgcaca gcgattcacc actgatcgca
     1981 aggccatggg taggtttggg taagggtaga gcgggaaacc gctcgtgaaa gatgcccttg
     2041 cggttagcga atccggcagc aacggaaggc ccctcttcgg aggggccttc tcctttccgt
     2101 tagactcgct ccccgcgcgc cggtagctca gtggatagag cgtcggcctc cggagccgaa
     2161 ggtcgcaggt tcgaatcctg tccggcgcgc caggctcgcc caagtgcgag gcatcggtca
     2221 tcatcgatcc atgccaggcc tgatccggcg tgaccggcgc gcgcgcgagg aagaggagga
     2281 gcgcctctcc gaaccggcgg cccgatcgac gcagagccgt ggccgcgtgc gccccgagga
     2341 gcccgatcga ttccgcaccg catacgagcg ggaccgggat cggatcatct actccaaggc
     2401 cttccggcgc ctgaagcaca agacgcaggt ctacctgaac cccgagggcg atcacttcgt
     2461 gacccgcctg acgcacaccc tgcaggtggc gcagatcgcc cgcgcccttg ccgccggcct
     2521 ctccctgaac gatccgctgg ccgaggcgat tgcgctgggc cacgacgtcg gccacacacc
     2581 cttcgggcac accggcgagg aggcgctgtc gccctacttc ccgcccgatg gctggcacca
     2641 cgccgcgcag agcgttcggg tctacgaggt gctcgaggac ctcaacctga cctgggaggt
     2701 ccgcgacggg atccgggccc attcctggaa gatcgagccg ccgccctcca cccaggaggc
     2761 tctctgcgtc cggtacgcgg atcgcatcgc gtacctggcc cacgacgcgc tggatgcgat
     2821 ccgggcgggc gtcctggagg ccgactcctt tccgcctgcg gcaatggcgc gcttcggcga
     2881 gccggggcgg gtctggatcg gagagatgat cgacgcggtg ctcgacaact cgctgcgggc
     2941 cggcgaggtg cgcatggatg acgagacgct ggacgtgatg cacgagctgc gcgcgttcat
     3001 gttcgagcgc gtctacctgg ccccatccct ccttcgtgag cagcgcgccg ccatcgacac
     3061 gctgggcgca ctggtcgacc accacctggc ccatcccgat cagatcccgg ccacctatcg
     3121 cgaggccgat gctcccctcg tcacgcaggt cgcggactac gtcgccggca tgaccgaccg
     3181 ctatgcgctg gcggccc
//
LOCUS       DATCQR010000153         4365 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_101080, whole genome shotgun sequence.
ACCESSION   DATCQR010000153 DATCQR010000000
VERSION     DATCQR010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4365
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101080"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /locus_tag="VIN32_05070"
     CDS             <1..408
                     /locus_tag="VIN32_05070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921983.1"
                     /translation="GQLGLAQVIVAAAHPSWKVSLEVPAAPGDPRAADLLLVGPTEVI
                     HIEIERALVDLQAQLRSAQLKREALAVREERPIRLVIAVPDTKATRARLAPFSEVLAR
                     TLPAQSRSIWRAIRAGEPLNQDGILFVRGPRRG"
     gene            complement(436..936)
                     /locus_tag="VIN32_05075"
     CDS             complement(436..936)
                     /locus_tag="VIN32_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010229758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HEY8921984.1"
                     /translation="MQVHSNDELGGLVDGLVAHEIPNRRGLEAWTALLRAHATLVRQL
                     ETDLKKETDLALADFDVLAQLALAGGKLRMTELADRALISRSGMTRRVANLIDEGLVA
                     RAGADADARVVVVSLTDAGVERLTETAPVHARGVAKLFVAQLDDDELAALRGALEKVT
                     LDCTFG"
     gene            1120..1659
                     /locus_tag="VIN32_05080"
     CDS             1120..1659
                     /locus_tag="VIN32_05080"
                     /inference="COORDINATES: protein motif:HMM:NF014628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HEY8921985.1"
                     /translation="MTVHRAADAAEAKRIVLGIIPPGSQVHHGASQTLAATGITDTFE
                     ESDDYEFLRPRLYSMDRKTQADEIRRLGAAPDVMLGSVHAVTETGSLITASMGGSQLG
                     PYVSGAGRVILVVGTQKIVSDLEEGLRRIDEYALPLEDARVMAAYGIHSAVNKVLIIN
                     REITPGRITVVLVDEVLGF"
     gene            1683..1868
                     /locus_tag="VIN32_05085"
     CDS             1683..1868
                     /locus_tag="VIN32_05085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921986.1"
                     /translation="MTTQMTARKERVTQVGDARARPMPDIDNGTHTVPDFAHAVRRHE
                     VIAAIERSAASGERVHR"
     gene            1904..3907
                     /gene="acs"
                     /locus_tag="VIN32_05090"
     CDS             1904..3907
                     /gene="acs"
                     /locus_tag="VIN32_05090"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016435970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HEY8921987.1"
                     /translation="MADERGQAPGSEPIEPAAEAATIENLLREERTFAPPAEFIRNAT
                     ANDPEIYRRTATEEGFRDFWTQEAARIDWIEPWTQLLDWQLPYAKWFVGGKLNISVNC
                     LDRHVAAGKGDKVAYYWEGEPGDKRTLTYKDLLDETCRLANALRELGVKKGDKVAIYM
                     PMIPELPIAMLACARIGAPHSVVFGGFSADALAGRIDDAQCTTVITADGGYRKGAAVH
                     LKGNADDAMARSPSVQNCVVVKRTGDPVEWQEGRDHWWHELVADQATEATPEPMDSED
                     LLYLLYTSGTTAKPKGIMHTTAGYLVGTAATHRLIFDIKPEDVYWCMADIGWVTGHSY
                     IVYGPLANATTGIIYEGAIDFPDKDRWAQIAARYHATILYTAPTAIRALMKYGQDAFA
                     GDDLSSLRLLGSVGEPINPEAWAWYWKYIGGERCPVVDTWWQTETGMILITPLPGLTI
                     TKPGSATFPFPGIKADVVDELGNSVPLGGGGYLVLERPWPAMLRGIYGDHDRYVETYW
                     SRYPGRYFAGDGCKRDDEGYYWLLGRVDDVMNISGHRISTTEVESALVSHPAVAEAAV
                     VGRTDPITGQAIFCYVILRAGFEASEELAAQLREHVAEVIGKLARPKQIMFTPDLPKT
                     RSGKIMRRLLRDIAEKRPLGDVTTLADEGIVSSIRDQSGGGED"
     gene            complement(4048..>4365)
                     /locus_tag="VIN32_05095"
     CDS             complement(4048..>4365)
                     /locus_tag="VIN32_05095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921988.1"
                     /translation="VDMVLDGPAGMVGVEVERALLDLQAQLRAAQLKRTAMAERTGRT
                     VRLVIGVLDTRRNRAAVLTHRSLLAAALPVPSKRIWACLRSGAPVLGDGLLWVRMPSR
                     AAP"
BASE COUNT          783 a         1518 c         1427 g          637 t
ORIGIN      
        1 ggtcagctcg ggctggcgca agtgatcgtg gctgcggcgc atccgagctg gaaggtgagc
       61 ctcgaggtgc cggcggcgcc cggcgatccg cgcgccgccg atctgctcct cgtcggccca
      121 accgaggtca ttcacatcga gatcgagcgc gcgctggtcg acctgcaggc gcagttgcgg
      181 tcagcgcagc tcaagcgcga ggcccttgcc gtccgcgaag aaaggccgat acgcctggtc
      241 atcgcggttc cggacacgaa ggcgacgaga gcacggctcg cgccattcag cgaggtcctt
      301 gcgcgaacgc ttccagccca gtcacgctcc atctggcgcg cgattcgagc gggcgagccg
      361 ctcaatcaag acgggatcct cttcgttcga ggaccgcgcc ggggctaacg ccgcgccggg
      421 gctagcgccg cgctctcagc cgaacgtgca gtccagggtg accttttcca gtgcgcccct
      481 gagggccgcg agctcgtcgt catccagctg agcgacgaac agcttggcga ccccgcgagc
      541 atggaccgga gcggtctcgg tcagtcgctc gacccccgca tcggtcagcg agaccaccac
      601 gacacgggcg tcggcgtcgg cgccagctcg cgcgacgagt ccttcgtcaa tcaaattggc
      661 gacccggcgc gtcatgcccg accgggagat gagcgcccgg tcggccagct ccgtcatgcg
      721 cagcttcccg ccagcgagcg cgagttgggc gagcacatcg aagtccgcca gggcgagatc
      781 cgtctccttc ttgaggtccg tctcgagctg gcggacgagg gtggcatgtg cccgaagcag
      841 ggcggtccag gcctccaggc cacgccgatt cgggatctcg tgggcaacca ggccgtcgac
      901 gagcccaccc agttcatcgt tcgaatgtac ttgcatatgc aaccagataa cggtaaggta
      961 cttgcggttg caactatacc agcaaggcaa ccggaaggaa agaacaatga tcaccgaagt
     1021 caagcccctc ttcgaggccg acgtcgcccg ccgtttcggc actgcgacgg atgaggcacg
     1081 agtcaatcga acgggtcgcc gccctccgag gcgaacggca tcaccgtcca ccgagccgcc
     1141 gatgctgccg aggcgaagcg gatcgtgctc ggcatcatcc cgcccggatc ccaggtccat
     1201 cacggtgcct cgcagacgct tgctgcgacc ggcatcaccg acacctttga ggagtcggat
     1261 gactatgaat tccttcggcc gcggctctac agcatggacc gcaagaccca ggccgatgag
     1321 atacgccgcc tgggcgcggc gccggacgtg atgctcggca gcgtgcacgc cgtcaccgag
     1381 accggatcgc tgatcaccgc ctcgatgggc ggcagccagc tcggaccata tgtcagcggc
     1441 gccggtcgag tcatcctggt ggtcgggacg cagaagatcg tctccgacct cgaggagggc
     1501 ctccggcgga tcgatgagta cgcccttccg ctcgaagatg cccgcgtcat ggcggcgtac
     1561 gggatccaca gcgcggtaaa caaggtcctc atcatcaacc gcgagatcac ccccggccgc
     1621 atcaccgtcg tcctggtgga cgaggtgctg ggcttctaag cccgtcgcaa ggagcgcgca
     1681 gcgtgaccac ccagatgacg gctcggaagg aacgggtcac ccaggtcggg gacgctcgcg
     1741 ctcgcccgat gcctgacatc gacaacggca cgcataccgt gccggatttc gcgcatgccg
     1801 tccggcgcca tgaggtgatc gccgccatcg agaggtcggc ggcatccggg gaacgcgtgc
     1861 accgctagcc ggcaacgccg acgccggggc tagaatccgg gcaatggcag acgagcgggg
     1921 ccaagcgccg ggatccgagc cgattgagcc cgccgccgaa gcggcaacca tcgagaacct
     1981 gctgcgcgag gagcgcacct tcgcgccgcc ggcggagttc atcagaaacg ccacggccaa
     2041 cgacccggag atctacaggc gcaccgcgac cgaggaaggg ttccgtgatt tctggacgca
     2101 ggaggccgcg cggatcgact ggatcgagcc ctggacgcag ctcctggact ggcagctccc
     2161 gtacgccaag tggttcgtcg gcggcaagct gaacatcagc gtcaactgcc tcgaccgcca
     2221 cgtcgcagcg ggcaagggag acaaggtcgc ctactactgg gagggcgagc cgggcgacaa
     2281 gcgaacgctg acctacaagg acctcctcga cgagacctgc cgcctcgcca acgccctccg
     2341 cgagctcggc gtgaagaagg gcgacaaggt cgccatctac atgccgatga tccccgagct
     2401 gcccatcgcc atgctcgcct gcgcacgcat cggggcgccc cactccgtcg tcttcggggg
     2461 cttctcggca gacgcgctgg ccggccgcat cgacgacgcg cagtgcacca cggtgatcac
     2521 cgccgacggc ggctatcgca agggcgcggc cgtccacctg aagggcaacg cggacgacgc
     2581 catggcgcgc agtccatcgg tccaaaactg cgtcgtggtc aagcgcaccg gcgaccccgt
     2641 ggaatggcag gaaggccgcg accactggtg gcatgagctg gtcgcggacc aggcaaccga
     2701 agccacccct gagccgatgg actccgagga cctcctctac ctgctgtaca cctcgggcac
     2761 gacggccaag cccaagggga tcatgcatac cacggcgggc tacctggtcg ggaccgcggc
     2821 cacccatcgg ctcatcttcg acatcaagcc cgaggacgtg tactggtgca tggccgacat
     2881 cggctgggtg accggccatt cgtacatcgt gtacgggccg ctcgccaacg cgaccaccgg
     2941 gatcatctac gagggggcga tcgacttccc cgacaaggac cgatgggccc agatcgccgc
     3001 ccggtaccac gccacgatcc tgtacaccgc ccccactgcc atccgcgccc tcatgaagta
     3061 cgggcaggac gccttcgccg gcgacgacct ctcctcgctg cgccttctgg gctcggtggg
     3121 tgagccgatc aaccccgagg cgtgggcctg gtactggaag tacatcggcg gggagcggtg
     3181 ccccgtggtg gacacctggt ggcagacgga gaccggaatg atcctgatca cgccgctgcc
     3241 tgggctcacc atcaccaagc cggggagtgc caccttcccg ttccccggca tcaaggccga
     3301 cgtggtggat gagttgggga actccgtgcc gctgggtggc ggcgggtatc tcgtgctcga
     3361 gcggccgtgg ccggccatgc tgcgcgggat ctacggcgac cacgaccgat acgtcgagac
     3421 ctactggtcg cgctaccccg ggcggtattt cgcgggggac ggctgcaagc gcgacgacga
     3481 gggctactac tggctcctcg gccgcgtcga cgacgtgatg aacatctccg gccaccgcat
     3541 ctcgacgacc gaggtggagt cggcgctcgt ctcgcacccc gctgtggccg aggctgcggt
     3601 ggtcggccgc accgacccga tcaccggcca ggcgatcttc tgctatgtca tcctgcgcgc
     3661 cgggttcgag gcctccgagg agctggcggc ccagctgcgc gagcacgtcg ccgaggtgat
     3721 cgggaagctg gcgcgcccca agcagatcat gttcacgccc gaccttccca agacccgctc
     3781 cggcaagatc atgcgtcgcc tgttgcggga catcgccgag aagcgtccgc tgggcgacgt
     3841 gaccaccctc gccgacgagg ggatcgtctc ctcgatccgc gaccagtcag gtgggggaga
     3901 ggactagccc cccgtcccgc ctgacccggc gggcataagc cagacttatg ctcctgcacc
     3961 gatgagggcc ctccggtgcg cctggataag gtgggcttat gcaacttcac cgatggccga
     4021 ccatcggtgc acgactatta gccaggctta tggcgctgcc cgcgaaggca tgcgtaccca
     4081 cagcagcccg tcgcccagca ctggcgcacc ggagcgaagg caggcccaga tcctcttcga
     4141 tggaacagga agagccgccg ccagcagcga ccgatgcgtg agcaccgcag cccgattgcg
     4201 ccgcgtgtcc agaacgccga tcaccaggcg aacggtccgg cccgtccgct cggccattgc
     4261 cgttcgcttg agctgcgctg cccgcaactg agcctggagg tcgaggagcg cccgctccac
     4321 ctccacgcca accattccag cggggccgtc cagcaccatg tcaac
//
LOCUS       DATCQR010000154         3148 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_179158, whole genome shotgun sequence.
ACCESSION   DATCQR010000154 DATCQR010000000
VERSION     DATCQR010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3148
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179158"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..1336
                     /locus_tag="VIN32_05100"
     CDS             2..1336
                     /locus_tag="VIN32_05100"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019523183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HEY8921989.1"
                     /translation="MPAPNMELIVSLAKRRGFVFPSSEIYGGMGGFWDYGPLGVELKN
                     NVKAAWWRQMVQLRDDIVGLDASIIMNPRVWEVSGHVAGFSDPMVDCRNCKLRFRADD
                     LKGPPSEIACPNCGNRGTLTEPRQFHLMFQTRVGPVEESASVAYLRPETAQGIFVNFA
                     NVATTARKRLPFGIAQIGKSFRNEITPGNFIFRDREFEQMEMEFFVHPSAEDEWFAYW
                     VDERLRWWTDVMGIGAERLRLRPHDPDELSHYSRQTTDIEYEFPMGWSELEGVADRTD
                     FDLKAHAEASGKSLSIFDEASGEHVTPFVIEPAMGVDRALLTVLLDAYEEQQVEGEKR
                     VVLHLRPSLAPIKVAVMPLLRNRPELVELGQRLAGELKLRMPATYDDTASIGKLYRRQ
                     DEIGTPYCVTVDVDTLTDGAATIRERDSMTQERVALDALPDRISELVAGGAA"
     gene            1323..2042
                     /locus_tag="VIN32_05105"
     CDS             1323..2042
                     /locus_tag="VIN32_05105"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY8921990.1"
                     /translation="MERPEVEDLAQVVAAATQRIAVRPGDDAAHEARARALLTLGRLE
                     EAEADAMDAVRLDPEEVRYRELLAEIQSRRGEHADAAAEYARLAQRDPRQVAWTVAEA
                     AERIEAADAGKGVEAARRAVRLDPGNFDAQLALARGLIHLGDPAAALPAAIRATDVRP
                     GDPGAREALADALWLAGQPSAAFEEFRALAEELTARDRERVLAKSRSVYRQGAGWLGR
                     LLAASDGLFAFALRRGWLRVR"
     gene            2039..2398
                     /locus_tag="VIN32_05110"
     CDS             2039..2398
                     /locus_tag="VIN32_05110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY8921991.1"
                     /translation="MKVGDLRERLAAAMASAMRRSEPEAVALTADRAKAMAVAMAGMD
                     PSAEVDPEALMVGTRQAGIILGFHPEHVRRLIRTGRLRAAIVGGDYRVLVSDLWPLIE
                     VRYRQPGRRRLAGRRPG"
     gene            2481..>3148
                     /locus_tag="VIN32_05115"
     CDS             2481..>3148
                     /locus_tag="VIN32_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HEY8921992.1"
                     /translation="MATTATKLIYTFPEGNAAMRALLGGKGAGLAEMTNAGLPVPPGF
                     TITTEACNAYYAAGKQLPPGLWDDVLAHMTALEQETGKKFGDPANPLLVSVRSGAAFS
                     MPGMMDTVLNLGLNPETVAGLIKLTDNERFGYDAWRRFVAMFGRIVLDIPATAFDEPF
                     DALKAKRGVKLDTELTADDLKEAADEYRAIVRTATAEDFPTDPYKQLELAVEAVFDSW
                     FGKRA"
BASE COUNT          512 a         1073 c         1098 g          465 t
ORIGIN      
        1 catgcccgcg ccgaacatgg agctcatcgt cagcctcgcc aagcgccgcg gcttcgtgtt
       61 cccgtcgagc gagatctatg gcggaatggg cggcttctgg gactacgggc cgctcggcgt
      121 cgagctcaag aacaacgtca aggccgcctg gtggcgccag atggtccagc tgcgcgatga
      181 catcgtgggt ctcgacgcgt cgatcatcat gaatccgcgg gtatgggagg tctccggtca
      241 cgtcgcgggc ttcagcgacc cgatggtcga ttgccgcaac tgcaagctcc gcttccgggc
      301 cgacgacctg aaggggccgc cgtcggagat cgcctgtccg aactgcggga atcgcggcac
      361 cctcaccgag ccgcggcagt tccacctgat gttccagacc cgggtcgggc cggtggagga
      421 gtcggcatcg gtagcctacc ttcgtcccga gacggcccag ggcatcttcg tcaacttcgc
      481 gaacgtggcg accacggcgc gcaagaggct gccgttcggg atcgcccaga tcggcaagag
      541 cttccgcaac gagatcacgc caggcaactt catcttccgc gaccgtgagt tcgagcagat
      601 ggagatggag ttcttcgtcc atccctcggc cgaggacgag tggttcgcct actgggtcga
      661 tgagcgcctc cgctggtgga ccgatgtgat gggaatcggc gccgaacgac tccggctgcg
      721 accgcacgat ccggacgagc tgtcgcacta cagccgccag acgaccgaca tcgaatatga
      781 attcccgatg gggtggtccg agctcgaggg cgtcgccgac cgaaccgact tcgacctgaa
      841 ggcgcacgcc gaggcatccg gaaagtcgct gtcgatcttc gacgaggcga gcggcgagca
      901 cgtgacgccg ttcgtgatcg agccggcgat gggggtcgat cgtgcactgc tgacggtcct
      961 gctcgacgcc tacgaggagc agcaggtcga gggcgagaag cgggtggtac tgcacctccg
     1021 cccgtcgctt gcgccaatca aggtcgcggt catgccgctc cttcgcaacc ggcccgagct
     1081 ggtggagctg ggacagcgcc tggcgggcga gctcaagctg cgaatgccgg ctacctacga
     1141 cgacaccgcg agcatcggca agctctaccg ccgccaggac gagatcggga cgccgtattg
     1201 cgtgacggtc gatgtcgaca ccctgaccga tggcgcagcc acgatccggg agcgcgacag
     1261 catgacccag gagcgggtgg cgctcgacgc gcttcctgac cggatcagcg agctggtggc
     1321 gggtggagcg gcctgaggtc gaagacctcg cgcaggtggt cgcagcggcc acccagcgga
     1381 tcgctgtccg cccgggcgac gacgccgcgc acgaggcccg ggcacgggcc ttgctgaccc
     1441 tcggccggct cgaggaggcg gaggccgatg cgatggacgc ggtccgcctc gaccccgaag
     1501 aggttcgcta tcgtgagctg ctggccgaga tccagtcgcg tcgcggggag cacgccgacg
     1561 cggcagccga gtacgcgcgg ctcgcgcagc gcgatccgcg ccaggttgcc tggaccgtag
     1621 ccgaggcagc cgagcgcatc gaggccgcgg acgcgggaaa gggggtcgag gcagcccgcc
     1681 gcgccgtccg gctcgatccg ggcaacttcg acgctcagct cgcgctggct cgcggcctca
     1741 tccatcttgg tgacccggcc gctgcgcttc ccgccgcgat ccgcgcaacg gacgtgaggc
     1801 cgggcgatcc tggcgcacgc gaagcattgg cggatgccct gtggctggcg gggcagccct
     1861 cggcagcctt cgaagaattc cgcgcgctgg ccgaagaact cacggcgcgt gatcgggagc
     1921 gagtcctcgc taagtccagg tctgtgtacc gccagggggc cggctggctc ggacgcctgc
     1981 tggctgcatc ggacgggctc tttgcctttg cccttcgacg cggctggctg cgggtgcgat
     2041 gaaggtcggc gacctgcgcg agcgcctggc cgccgcaatg gcgagcgcga tgcgacgctc
     2101 cgagccagag gctgtcgcgc tgaccgccga tcgagccaag gcgatggccg tcgccatggc
     2161 cggcatggac ccgtcggccg aggtggaccc ggaggcgctg atggtgggca ccaggcaggc
     2221 ggggatcatc ctcggcttcc acccggagca cgtgcggcga ctgattcgca ccggacgcct
     2281 gcgcgcggcg atcgtcggcg gggactaccg ggtcctggtc tcagacctgt ggccgctgat
     2341 cgaggtccgg taccggcagc caggccgccg cagactcgcg ggtcgccggc ccggctgagg
     2401 cccgccgtcg ccggacgagc gatcccggcg ggtagaatca tgctcccgcg accctcaggt
     2461 ggaggatcct cgaccgccac atggccacga ccgcaacgaa gctgatctac accttccccg
     2521 aggggaacgc tgccatgcgt gcgctgctcg gcggtaaggg cgccgggctc gccgagatga
     2581 cgaacgcggg gctccccgtg ccgcccggct tcacgatcac caccgaggcc tgcaacgcct
     2641 actacgcggc cggcaagcag ctcccgccgg gcctgtggga cgacgtcctc gcgcacatga
     2701 ccgcactcga acaagagacc ggcaagaaat tcggcgaccc cgccaacccc ctgctggtca
     2761 gcgtccgctc cggcgccgct ttctcgatgc ccgggatgat ggacaccgtc ctcaacctgg
     2821 gcctgaaccc tgagactgtc gccgggctca tcaagctcac ggacaacgag cgcttcggct
     2881 acgacgcctg gcgccgattc gtggcgatgt tcggccggat cgtgctcgac atcccggcca
     2941 ccgccttcga cgagccgttc gacgcgctca aggccaagcg tggcgtgaag ctcgacaccg
     3001 agctcaccgc ggacgatctc aaggaggcgg ccgacgagta tcgggccatc gtgcggaccg
     3061 ccaccgctga ggacttcccg accgacccgt acaagcagct cgagctggct gtggaggccg
     3121 tcttcgactc ctggttcggg aagcgggc
//
LOCUS       DATCQR010000155         7194 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_24308, whole genome shotgun sequence.
ACCESSION   DATCQR010000155 DATCQR010000000
VERSION     DATCQR010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 7194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7194
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24308"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..373)
                     /locus_tag="VIN32_05120"
     CDS             complement(<1..373)
                     /locus_tag="VIN32_05120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8921993.1"
                     /translation="MTPARTLRLIWFVTWFNVRILLASEFFILTTFIAPLIFATLAFF
                     LFRSGGGVAGQTLLYVALGAGMMGVWSTTLFGCGGAIAWQRWEGTLELLVNAPSRYDY
                     ILVGQTLGAVVFGFYGILTTLV"
     gene            complement(382..1413)
                     /locus_tag="VIN32_05125"
     CDS             complement(382..1413)
                     /locus_tag="VIN32_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020522304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8921994.1"
                     /translation="MAASGGGTLAIEAIDLVRTYQTHTGVIRRRPMTVEAVRGISFEV
                     RPGELFGLLGPNGAGKTTTIKMLITLLLPTSGTARVLGFDVVRDTTAVRQHIGYVFGG
                     DRGLYDRVSGLDNLRYFAELYGVEPSEQKRRIAALLELVGLTGRENEKVEGYSRGMRQ
                     RLHIARGLLHDPEVIFMDEPSIGLDPVGARELRQTVRSLVDSGKTVLLTTHYMFEADA
                     LCDRIAVINAGRIVALGTGADLKDAIAERTIVEIETFGVTDEAVERLRRDDDVSAINM
                     EERDQSQILLVQSERGAHITQRLLSLLGDTRVGKVVAREPTLEDAYVHLIATSGDDEA
                     RAARAASVQ"
     gene            complement(1413..2408)
                     /gene="corA"
                     /locus_tag="VIN32_05130"
     CDS             complement(1413..2408)
                     /gene="corA"
                     /locus_tag="VIN32_05130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HEY8921995.1"
                     /translation="MPTNSNRTCLARLPGGELKELSAKEHDTIDGLRKKADHVVWLDI
                     ASPTADDLKLLRDEFSVHPLALEDIEKRRQRPKLDTYSEQQIIVTYEVLEPSQADQHF
                     DLGELHLFAGPGYVVSVRWHGSPAIEEVDRRFRDRPDAVARTPGSLVYAILDAAVDGY
                     FPLLDRLTDKIEGLEERILAGGRSNDTLRIILGLKRKLLELRRHLSPQRDVANALLRR
                     DLPLVDDTSAPYFQDLYDHTIRVLDQLDLQRDLLASALEANLSVTSNNLNAIMKRLTA
                     FTVILMVPTLIAGVYGMNFHFMPELSWPFGYPLALVVMLATMVGGALFFRARDWF"
     gene            complement(2436..2867)
                     /locus_tag="VIN32_05135"
     CDS             complement(2436..2867)
                     /locus_tag="VIN32_05135"
                     /inference="COORDINATES: protein motif:HMM:NF015917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8921996.1"
                     /translation="MEDWESLSAEVGSRPAAVSWAENEMQVFFIGTDGQLWDTYWDGA
                     VWHDRHPHGGELAGSPAACSWGADRIDIFARGRDGTLQHRWYVPEGWQPWESLEIRID
                     GDPAACSWGRDRIDLFVRSDAGALLHGWWDGQRWSFTQRPT"
     gene            2956..4335
                     /locus_tag="VIN32_05140"
     CDS             2956..4335
                     /locus_tag="VIN32_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HEY8921997.1"
                     /translation="MDQWKTIIEPFRIKSVEPIRMTSVAEREGHLREAGYNLFNLHAA
                     DVIIDLLTDSGTGAMSAEQWAGIQRGDESYAGSPSWFRFLESVQILFPFKHVIPTHQG
                     RAAEKILFSVIGGPGKAIPSNTHFDTTRANVEFSGAEAVDLVIEEGRHPSVIHPFKGN
                     MDLDALDAFIRDRGAQAVPVVMVTVTNNSGGGQPVSLENLRGVREICDRYHLPLFLDA
                     CRFAENAWFIKQREPGQAGRSIPDIVREMASMADGMTMSAKKDAIANIGGWLALNDDR
                     WAEAARNLLILTEGFPTYGGLAGRDLEAIAQGLREVVDEDYLRYRIRSTAYLGEALVE
                     SSVPCVQPIGGHAVYLDAAALLPHIPPLQYPGQALAIALYREGGIRGCEIGTVMFGMH
                     PDGTESPGPMELVRLAIPRRVYTQSHIDYVIEVVRSVAADASALRGYRIVEQPAQLRH
                     FTARFEPLS"
     gene            4352..5377
                     /locus_tag="VIN32_05145"
     CDS             4352..5377
                     /locus_tag="VIN32_05145"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HEY8921998.1"
                     /translation="MTTLARRFTTWRYVMNHANMPVGVEMDWLSKWLIVTRAAVFSMT
                     ATSGLIGGLLAIGTPGLSVNYWYLTLSVVGLVIAHASNNMINDYFDLEGGVDTDDYVR
                     ALYAPHPILSGWLSKAQLRNAILLMNVLDLAILVFLVWVRGPLVATFALSGLFISVFY
                     VAPPIKLKHRGLGEPGVFVVWGPLMIAGTFFVATGVLPGWVWIASLPYAILVTTVLFG
                     KHIDKISADTAKGIHTLPVILGETRARWVAQALMIAFYPIVIGSALIGWIGPFVMLVV
                     LGIPLLLTVLRQFSSPRPESPPHSYVGWPLWFVGGAFRHTRRAGGLLILGLFLNAILP
                     GLRLPWT"
     gene            5396..6124
                     /locus_tag="VIN32_05150"
     CDS             5396..6124
                     /locus_tag="VIN32_05150"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEY8921999.1"
                     /translation="MFPIGDENLPGRGLAWVTLGLIAVNVAVFIFLQGGSGDNTFTYG
                     WSSIAREITTGVDLTHAVPVVIDGTQYLIPQAPGPDPIYLTLLSSMFMHGSWLHLGGN
                     MLFLWIFGDNVEHRAGPLLYLVAYLAAGLVGSLAQILSDTTSQIPSLGASGAISGVLG
                     AYLILFPRNRVTAFVIRFLVQIPALVAIGMWIAIQVISSLADPTGTGGVAYMAHIGGF
                     GAGVLAGLLFRALPDRGAGPAASR"
     gene            6121..6942
                     /locus_tag="VIN32_05155"
     CDS             6121..6942
                     /locus_tag="VIN32_05155"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HEY8922000.1"
                     /translation="MSRTADHGLFGPDSISWRIDREVLVLAGGTCALLMQLAHPAVAA
                     GVAEHSDFRADPFARLRRTLAATHAVVFGSTPRAERTIRRMNAIHASVAGAVPETGEA
                     YSALDPALLLWVHATLVDTAVRVYDRFVAPLSDAEAEAYHTEARQIAIRLGASDASLP
                     DTLTDLRTEMDRLIRSGEVTVSATARSLAPAVLYPTRFPPQPVWDLAHLVSFSVLPPA
                     IRRGYGISWSRRRERGVRLLASASRRLLPLVPDLLRHVPAARSAEARLLRASAAR"
     gene            7028..>7194
                     /locus_tag="VIN32_05160"
     CDS             7028..>7194
                     /locus_tag="VIN32_05160"
                     /inference="COORDINATES: protein motif:HMM:NF014665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0016653 - oxidoreductase activity, acting
                     on NAD(P)H, heme protein as acceptor [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HEY8922001.1"
                     /translation="MNRFAKLAVAASIATYVLIVVGGLVRATDSGLGCPDWPACFGKW
                     VPPAELHAWICL"
BASE COUNT         1106 a         2504 c         2352 g         1232 t
ORIGIN      
        1 acaccagcgt ggtcaggatt ccatagaagc cgaagaccac ggcgccgagg gtctggccga
       61 ccaggatgta gtcatagcgg gagggggcgt tcacaaggag ctccagcgtc ccctcccagc
      121 gctgccaggc gatggcacct ccgcagccga acagggttgt gctccagacg cccatcatgc
      181 ctgccccaag ggcaacgtag agaagcgtct gcccggccac gcctccgcct gaccggaaca
      241 ggaagaaggc cagggtggcg aagatgagtg gcgcgatgaa ggtggtcagg atgaagaact
      301 cgctggccag caggatccgc acgttgaacc aggtgacgaa ccagatcaat cgcagcgttc
      361 tcgccggagt catcgcgtca cctactgaac cgatgcggcc cgcgccgctc gggcctcgtc
      421 gtcgccgctt gtcgcaatga ggtgaacgta ggcgtcctcg agggtcggtt cgcgtgcgac
      481 cactttgccg acccgcgtgt cgccgaggag agagaggaga cgctgggtga tgtgtgcgcc
      541 ccgctcgctc tggaccagga ggatctggct ctggtcgcgc tcctccatgt tgatggcgct
      601 gacatcgtcg tcccggcgca gccgctcgac ggcctcgtcg gtcactccga aggtctcgat
      661 ctcgacgatg gttcgctcgg cgatcgcgtc cttcaggtcc gcgccggtcc cgagcgcgac
      721 gatgcggccc gcgttgatga cagcgatgcg gtcgcaaagt gcatcggcct cgaacatgta
      781 gtgagtggtc aggaggacgg tcttgccgga gtcgacgagc gagcggacgg tctggcgcag
      841 ctcacgcgcc cccaccgggt ccaggccgat cgaaggctca tccatgaaga tcacctctgg
      901 atcgtggagc agcccgcgcg cgatgtggag gcgttgacgc atcccgcggc tgtatccctc
      961 caccttctcg ttctcccgcc ccgtgagccc gacgagctcc agcagggcgg cgatccggcg
     1021 cttctgctca gacggctcga cgccatacag ctcggcgaaa tagcgcaggt tgtcgaggcc
     1081 gctgacccgg tcgtagagcc cgcgatcccc gccgaagacg tagccgatgt gctgacgaac
     1141 cgcggtcgta tcgcgcacca catcgaatcc caggaccctt gccgtcccgg aggtcggtag
     1201 caggagcgtg atcagcattt tgatcgtggt cgtcttgcca gcgccgtttg gccccagcag
     1261 gccgaagagc tcgccgggtc gcacctcgaa gctgatgccg cgcacagcct cgaccgtcat
     1321 cggccggcga cggatgaccc cggtgtgggt ttggtaggtc cgcacgaggt cgatcgcctc
     1381 gatggcgagg gtgccacccc cggaagcagc catcagaacc agtcgcgagc gcggaagaag
     1441 agcgccccac cgaccatggt cgccagcatc accaccagcg ccagcggata gccgaacggc
     1501 cagctcagct cgggcatgaa gtggaagttc atgccgtaga cgcccgcgat cagggtggga
     1561 accatcagga tcacggtgaa ggcggtcagc cgcttcatga tcgcgttcag gttgttgctg
     1621 gtgaccgaca ggttggcctc aagcgccgat gccagcaggt cgcgctgcag gtccagctgg
     1681 tcgagcaccc ggatggtgtg gtcgtacagg tcctggaaat agggagccga tgtgtcgtcg
     1741 accagcggca ggtcccggcg caggagggcg ttggccacgt cccgctgcgg ggagaggtgc
     1801 cgtcgcagct cgagcagctt gcgcttcagg cccaggatga tgcgcagggt gtcgttgctc
     1861 cgcccgcccg ccaggattcg ctcctcgaga ccctcgatct tgtcggtgag tcggtcgagc
     1921 aggggaaagt agccgtccac cgcggcgtcg aggatggcgt acaccagtga gcccggcgtc
     1981 cgggccacgg cgtcgggccg atcgcggaag cgccgatcga cctcctcgat ggcgggcgac
     2041 ccgtgccagc ggacgctgac gacgtagccg ggtcccgcga agaggtggag ctcgccgagg
     2101 tcgaagtgct gatcagcttg ggacggctcg agcacctcgt aggtcacgat gatctgctgc
     2161 tcggagtacg tatcgagctt ggggcgctgc cgtcgcttct cgatgtcctc cagggccaga
     2221 ggatggacgg agaactcgtc gcggagcagc ttcaggtcgt cagcggtggg cgacgcgata
     2281 tcgagccaca cgacatggtc agccttcttc cgcaggccat cgattgtgtc gtgctccttg
     2341 gcgctcagct ccttcagctc accgccgggg agtcgcgcaa ggcaggtgcg gttcgagttc
     2401 gtaggcatcg ggcgtgagtc tacccgcccc ggcattcagg tcggccgctg ggtgaaggac
     2461 cagcgctggc cgtcccacca gccgtgcagc agcgcccctg cgtcgcttcg gacgaagagg
     2521 tcaatccgat cccgacccca gctgcaggcc gccggatccc cgtcgatccg aatctcgagc
     2581 gactcccaag gctgccagcc ttccggcacg taccagcgat gctggagcgt cccgtcccgc
     2641 cctcgagcga agatgtcgat ccgatcagct ccccagctgc aggccgcggg gctgccggcc
     2701 agttccccgc cgtgtgggtg gcgatcgtgc cagaccgcgc cgtcccagta ggtatcccac
     2761 aactggccat cggtcccgat gaagaagacc tgcatctcgt tctcggccca gctcactgcc
     2821 gccgggcgcg acccgacctc ggcgctcaac gactcccagt cctccatcgc ctctcctctc
     2881 cagagctccg cgcgagagcg ccatgatggc ccgcccccgc tccgcacgct caggtccagc
     2941 gcctatgctc ccgtcgtgga tcaatggaag acgatcatcg agccgttccg gatcaagagc
     3001 gtggagccga tccggatgac ctcggtggcg gagcgcgagg ggcacctgcg cgaggcaggc
     3061 tacaacctgt tcaacctcca cgcggccgac gtgatcatcg acctgctgac ggactctggc
     3121 accggagcga tgtcggccga gcagtgggcc ggaatccagc gaggcgacga gtcgtatgcg
     3181 ggctcgccat cctggttccg cttcctggag agcgtgcaga tcctcttccc gttcaagcac
     3241 gtcatcccga cgcaccaggg cagggccgcg gagaagatcc tcttctcggt catcggcggc
     3301 cccggcaagg cgattcccag caacacgcac ttcgacacga cccgggccaa cgtcgagttc
     3361 agcggcgcgg aggccgtcga cctcgtgatc gaggaggggc gccacccatc ggtcatccat
     3421 ccgttcaagg gcaacatgga cctcgacgcc ctggatgcgt tcatccgtga ccgcggagcg
     3481 caagccgtcc cggtggtgat ggtcaccgtc accaacaact cgggcggcgg gcagccggtc
     3541 agcctcgaga acctgcgggg cgtgcgcgag atctgcgacc gctaccacct gccgctcttc
     3601 ctcgacgcct gccgctttgc cgagaacgcc tggttcatca agcagcggga gcctggccag
     3661 gcgggccgat cgatccccga catcgtgcgc gagatggcat cgatggccga tggaatgacg
     3721 atgtccgcca agaaggacgc gatcgccaac atcggtggct ggctggcgct caatgacgat
     3781 cgctgggccg aagcagcgcg caacctgctc atcctgaccg agggcttccc cacctacggc
     3841 ggcctggcgg gtcgcgacct ggaggcgatc gcccaggggc tgcgcgaggt cgtcgacgag
     3901 gactacctgc gctaccgcat ccgctccacc gcctacctcg gcgaggccct tgtcgagtcc
     3961 agcgtcccat gcgtgcagcc gatcgggggg cacgccgtgt acctggacgc cgccgcgctc
     4021 ctaccccaca tccctccgct gcaatacccc ggccaggcgc tggcgatcgc cctctaccgg
     4081 gaggggggca ttcgcggctg cgagatcggg acggtcatgt tcgggatgca ccctgacggg
     4141 accgagagcc ccggaccgat ggagctggtg cgcctcgcca tcccgcggcg ggtctacacc
     4201 cagtcgcaca tcgactacgt gatcgaggtc gtccgctcag ttgcagcgga tgcctccgca
     4261 ctgcggggct atcgaatcgt cgagcagccg gcacagctcc gccacttcac cgcccgcttc
     4321 gagcccctct cctgagccgt acaatctcgc catgaccacg ctcgcgcggc gattcacgac
     4381 ctggcgctac gtcatgaacc acgccaacat gccagtgggc gtggagatgg actggctcag
     4441 caagtggctg atcgtcaccc gcgccgcggt cttcagcatg accgccacct cgggcctgat
     4501 cggtggcctg ctcgcgatcg gcacccccgg cctctcggtg aactactggt acctgacgct
     4561 gtcggtcgtc gggctggtga tcgcccacgc gtcgaacaac atgatcaacg attacttcga
     4621 cctggagggc ggcgtcgaca ccgatgacta cgtgcgcgcc ctgtatgcgc cgcacccgat
     4681 cctctccggc tggctctcga aggcgcagct gcgcaacgcg atcctactga tgaacgtcct
     4741 cgacctcgcg atcctggtgt tcctggtctg ggtgcgtggg cccctggtcg caacctttgc
     4801 cctcagcggc ctcttcatca gcgtcttcta cgtcgccccg ccgatcaagc tcaagcatcg
     4861 cgggctcggc gagccgggtg tgttcgtggt gtggggcccg ctgatgatcg ccgggacatt
     4921 cttcgtggcg acgggcgtgc tccccggctg ggtctggatc gcgtcgctgc cctacgcgat
     4981 cctggtcacg acggtcctct tcgggaagca catcgacaag atctccgcgg acaccgccaa
     5041 ggggatccac accctgcccg tcatcctggg cgagacacga gcccgctggg tggcgcaggc
     5101 cctgatgatc gcgttctacc cgatcgtcat tggctcggcg ctcatcggct ggattggccc
     5161 gttcgtgatg ctcgtggtgc tcggcatccc gctgctgttg acggtgcttc gccagttctc
     5221 ctcgccgcga cccgagtcgc cgccgcacag ctacgtcggc tggccgctct ggttcgtggg
     5281 cggggccttc cgccacacgc gacgtgcggg cggcctcctg atcctcggcc tcttcctgaa
     5341 cgcgatcctg cctgggctcc gcctcccctg gacctagcct ccatactcct gaccgatgtt
     5401 tccgatcggt gacgagaacc ttcccggcag agggctcgcc tgggtcaccc tcggcctgat
     5461 tgccgtcaac gtggcggtgt tcatcttcct ccagggcggc agcggggata acaccttcac
     5521 ctacggctgg agctcgatcg cgcgcgagat caccaccggc gtcgacctga cccatgccgt
     5581 gccggtcgtg atcgatggca cccagtacct catcccgcag gcgcccggcc cggatccgat
     5641 ctacctgacg ctgctgtcga gcatgttcat gcacggcagc tggctgcacc tcggcggcaa
     5701 catgctcttc ctgtggatct tcggcgacaa cgtcgagcac cgcgccggcc cgctcctgta
     5761 cctggtggca tacctggcgg ccggcctggt cgggtccctc gcgcagatcc tgagcgacac
     5821 cacctcgcag atcccctcgc tcggggcgtc gggcgccatc tccggtgtgt tgggggccta
     5881 cctgatcctc ttcccgcgaa accgggtgac cgccttcgtc atccgcttcc tggtgcagat
     5941 tccggcgctg gtcgccatcg gcatgtggat cgccatccag gtgatctcga gcctcgccga
     6001 tccgaccggg accggtgggg tcgcctacat ggcccacatc ggcgggtttg gggccggcgt
     6061 cctggccggc ctcctgttcc gggcactgcc cgatcgcggc gcaggtcccg cggcatccag
     6121 atgagccgga cggccgacca tggcctgttc ggccccgaca gcatctcctg gcggatcgac
     6181 cgggaggtgc tcgtcctggc cggtggcacc tgcgccctcc tgatgcagct cgcgcatccg
     6241 gcggtggcgg cgggcgtggc ggagcactcg gactttcgag ccgatccctt tgcgcggctg
     6301 cgacggaccc tggcggcgac gcatgccgtc gtcttcggca gcacgccccg cgcggagcgc
     6361 acgatccgcc gcatgaacgc cattcacgcc tcggtagccg gcgccgtgcc cgagacgggg
     6421 gaggcctact cggcgctcga tccggcgctg ctgctgtggg tgcatgccac gctggtggac
     6481 accgccgtcc gtgtctacga ccgcttcgtc gcccctctct ccgacgcgga ggcggaggcc
     6541 tatcacaccg aggcgcgtca gatcgccatc cgcctcggcg cctccgacgc gtcgcttccg
     6601 gacacgctga cggacctgcg gacggagatg gaccggctga ttcgcagtgg cgaggtcacg
     6661 gtgagcgcca ccgcccgcag tctcgcaccc gccgtgctgt acccgacccg ctttccgccg
     6721 caaccggtgt gggatctcgc ccacctcgtc tcgttctcgg tgctgccgcc cgccattcgc
     6781 cgcggctacg gcatcagctg gtcccgccgc cgcgagcgag gggtccgcct gctcgcatct
     6841 gccagcaggc ggttgctgcc tctggtgccg gacctgctgc gccacgtccc ggcggcccgt
     6901 tccgcggagg ccaggctgct acgggccagc gcggcacggt gacgcggtcg gttcgggtcg
     6961 gtatgatgcc gccagcccat tccggcactg agatgaaccg ccgccgcggc gtgattcgag
     7021 gacctcgttg aaccgattcg cgaagctcgc cgtagccgcg tcgatcgcaa cctatgtgct
     7081 gatcgtggtc ggcgggctgg tacgtgccac tgattcagga ttgggctgcc cggattggcc
     7141 ggcctgcttc gggaagtggg tcccacctgc ggagctgcac gcgtggatct gtct
//
LOCUS       DATCQR010000156         5290 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_163627, whole genome shotgun sequence.
ACCESSION   DATCQR010000156 DATCQR010000000
VERSION     DATCQR010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5290
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163627"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1125
                     /locus_tag="VIN32_05165"
     CDS             <1..1125
                     /locus_tag="VIN32_05165"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEY8922002.1"
                     /translation="IAPDARLVSVKVADQTGASDVSQVIAGIDWVVQHRHDNGLNIRL
                     INLSFGTDSRQSYINDPLAFAAEQAWRAGITVVVSAGNDGQAATGLADPAYDPLLLAV
                     GAVDTNNSLLRSDHTIAAFSDYGSVSRGPDVVAPGVHIASLLVPGSYIDRRYGAAGMV
                     GDRFLRGSGTSQAAAVTTGAAALLLQENPGATPDQLKKLLTSSAEDLDNAAVASQGSG
                     EINLKVGFSTALSKTVQHFIPSIGIGSLELARGTNHLTAGGVTLTGEKDIFGATFNTK
                     KMAVAEITDSAWTGGVFNGNSWTGNSWTGNSWTGNSWTGNSWTGNSWTGNSWSGNSWT
                     GNSWTGNSWTGNSWTGNSWTGNSWSGNSWSGNSWTSADWS"
     gene            1160..3508
                     /locus_tag="VIN32_05170"
     CDS             1160..3508
                     /locus_tag="VIN32_05170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8922003.1"
                     /translation="MARLLRAGALGRLKETAVRVWLFALVLDAAAIALFLGPLQHLPR
                     FAGHGEISWPLLAGFFLVGELLAVHLEVRGSAHTYSLSEIPTVFALFFASPSEVVICR
                     IIGSAVALVFVRRNSPVKLAFNLGLFTFETSIAVWFFHTIVPASQPVGPAGWSAAVFA
                     MLASSVVAVIVINMVIALHQGRETFLQLGEALSLGVTVTAVNSCLALLFVTVIWHQPQ
                     SAWLILVPVAALYFAYRSAQSERRKRKSITHLFNLGRELQGSVQTDTVIRTLTNHALD
                     MFNADWVEVLVAPEVAGGAWRRAVQAGAEQAEKLDSLRPQRGVEDWFRQIMASTGGAP
                     NRYEASDTPYLESRDQRQVMVTNLRGETSSLGLLLIGRHKLGFRGFDSEEKRLLETLA
                     SQASAALETARLAEQLRAAEQVSSRLGRIIDQSSSDVYLVESQSLQLLQANRSATERL
                     GYTADEMLRLSLADLLFEGDRKCLEEQLAPLRSGIQAEVLIESNQRRRDGTSYPVEAR
                     IQVTQSTEPELLLVISQDVTERNEALEARQESREKSRFLAAMSHELRTPLNSILGFSQ
                     LLLTDRFGSLSQKQAKYVANVESSGQHLLLLINDLLDLSKVQAGKMDIALSAVGLFPL
                     IEESVTKMVELAEAKGLDLEVGDDAGLIVVADPLRLRQILLNLLSNAIKFTDKGLVRI
                     DAVACGEEVRLDITDTGVGIDAEDLERMFEEFTQAKTGLSKSAQGTGLGLPISRRLAE
                     LMGGRIEVASTVGQGTTFSLFLPATGVGEIGKAPRRVISAVG"
     gene            complement(3527..4771)
                     /locus_tag="VIN32_05175"
     CDS             complement(3527..4771)
                     /locus_tag="VIN32_05175"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013375486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEY8922004.1"
                     /translation="MDTLKAALTLVDPASVGFDAARLSRIDDHLRAYVEDGRLPGLQV
                     MVSRRGKVVHLSSFGKRDKEAGLPVEADTIFRIYSMTKPITSVAAMMLFEQGAFELTT
                     PVSRFIPSFADARVYLRGNSTKPLTAPVAEPVRIWHLLTHTAGLTYGFLHAHAVDEAY
                     RAAGYEWGWPEGTDLATACDTWARIPLAFQPGTEWNYSVATDVLGRVIEVASGQSLDS
                     FFADRIFKPLGMEDTHFSVPEADRDRLAALYAVAPGIPGPIRYDVMGEAALKPPSLFS
                     GGGGLVSTTADYHRFTQLLLGRGELDGTRLLAPKTIGLMAQNHLPGGHDLETVGRPLY
                     AETNFTGIGFGLGFSVVLDPIAAKTLSNRGELAWGGAASTAFWVDPVDEVVVILMTQL
                     LPSSTYTIRTELRQLVYQALID"
     gene            5110..>5290
                     /locus_tag="VIN32_05180"
     CDS             5110..>5290
                     /locus_tag="VIN32_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007071904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY8922005.1"
                     /translation="MELKDKVVIVTGGAGGIGSALGRRFAAEGARAVVLADRDGARAE
                     DAAAAIGPAARGADCD"
BASE COUNT          858 a         1841 c         1756 g          835 t
ORIGIN      
        1 atcgccccgg acgcccgcct ggtgtccgtc aaggttgccg atcagaccgg cgcaagcgac
       61 gtctcgcagg tgatcgccgg catcgactgg gtcgttcagc accggcacga caacggcctc
      121 aacatccgct tgatcaacct ctccttcggc accgacagca ggcagagcta catcaacgat
      181 ccgctcgcgt tcgcggcgga gcaggcctgg agggcgggca tcacggtggt cgtctcggcc
      241 ggcaacgacg gccaggcggc gaccggtctc gccgatccgg cctatgaccc actcctgctc
      301 gcggtcggag cggtggacac caacaactcg cttctccgct ccgaccacac catcgcggct
      361 ttctcggact acggctccgt gagccgcggc cctgacgttg tggcgccggg cgttcacatc
      421 gcgagcctgc tcgttcccgg ctcctacatc gaccgccgct acggcgcggc cggcatggtc
      481 ggcgaccgct tcctgcgcgg cagcggtacg tcacaggcgg cggcggtcac gaccggggcg
      541 gcagccctcc tcctccagga gaatcccggg gcgacgccgg atcagctcaa gaagctgctg
      601 acctcgtccg cagaggacct cgacaacgcc gccgtcgcga gccagggcag tggtgagatc
      661 aacctcaagg ttggcttctc caccgcgctc agcaagaccg tgcagcactt catcccgagc
      721 atcggcatcg gctccctcga gctggcccgc ggcaccaatc acctcactgc gggaggcgtc
      781 acgctgaccg gcgagaagga catcttcggc gcgaccttca atacgaagaa gatggcggtt
      841 gccgagatta ccgacagcgc ctggaccggc ggcgtcttca acggcaactc gtggaccggc
      901 aactcctgga ccggcaactc gtggaccggc aactcgtgga ccggcaactc ctggaccggc
      961 aactcctgga ccggcaactc ctggagcggc aactcgtgga ccggcaactc gtggaccggc
     1021 aactcgtgga ccggcaactc gtggaccggc aactcgtgga ctggcaactc ctggagcgga
     1081 aactcctgga gcggaaactc ctggacaagt gccgattgga gttgacccgg taacgtcgag
     1141 gatcggagtc cttacggccg tggcgcgcct gctgagagca ggcgcgctcg gtcgtttaaa
     1201 agagactgcg gtccgggtgt ggctgttcgc cctcgtcctc gacgcggccg cgatcgcact
     1261 cttcctgggg ccgctccagc acctgccccg cttcgcgggc cacggcgaga tcagctggcc
     1321 cctgctggca ggcttcttcc tggtcggcga gctgctcgcg gtgcacctcg aggtgagggg
     1381 cagcgcccac acctattcgc tgagcgagat cccgacggtg ttcgcgctct tcttcgcgag
     1441 tccctcagag gtcgtcatct gccggatcat cggttcggcc gtggcgctcg tgttcgtccg
     1501 ccgcaacagc ccggtgaagc tcgccttcaa cctcggcctg ttcacattcg agacgtcgat
     1561 cgcggtctgg ttcttccaca ccatcgtgcc ggcttcgcag ccagtcggcc cggcgggctg
     1621 gtcggcggcg gtcttcgcga tgctcgccag cagcgtcgtc gcggtgatcg tgatcaacat
     1681 ggtgatcgcc ctacaccagg gccgcgagac gttcctccag ctcggcgagg cactcagcct
     1741 cggcgtcacc gtgaccgccg tcaacagctg tctcgcgctc ctcttcgtca ccgtcatctg
     1801 gcaccaaccg caatccgcct ggctgatcct ggtgcccgtc gcggccctct acttcgccta
     1861 ccggtccgcc cagtcggaac ggcgcaagcg gaagagcatc acccacctct tcaacctcgg
     1921 ccgggagctg caggggtcgg tccagaccga caccgtcatc cggacgctga cgaaccacgc
     1981 gctggacatg ttcaacgccg actgggtcga ggtcctggtc gccccggagg ttgccggcgg
     2041 tgcctggcgc cgcgcggtac aagcgggcgc cgagcaagcc gagaagctgg acagtctgag
     2101 gccgcagcgg ggtgtcgagg actggttccg gcagatcatg gcctcgaccg gtggagcacc
     2161 caaccgatac gaggcgagcg acacgccgta cctcgagtcg cgcgaccagc gccaggtcat
     2221 ggtgaccaac ctgcgcggcg agacgagcag cctcggcctg ctcctgatcg gccgccacaa
     2281 gctcggcttc agggggtttg actcggagga gaagcggttg ttggagacgc ttgcgagcca
     2341 ggccagcgcg gcgctcgaga cggcgcggct ggccgagcag ctgcgcgccg ccgagcaggt
     2401 ctccagccgg ctgggccgga tcatcgacca gtcgtccagc gacgtctacc tcgtcgagag
     2461 ccagtcgctc cagctgctcc aggcgaaccg gagcgcaacc gagcgtctcg gctacacagc
     2521 cgacgagatg ctcaggctgt cccttgccga cctgctcttc gagggcgacc gcaagtgcct
     2581 ggaggagcag ctggcgccgc tccgttcggg aatccaggcc gaggtcctga tcgagtccaa
     2641 ccagcgccgc cgggacggca ccagctaccc cgtcgaggct cgcatccagg tcacgcagtc
     2701 gaccgagccg gagctcctcc ttgtgatctc ccaggacgtc accgagcgga acgaggctct
     2761 cgaggcccgc caggagagcc gcgagaagtc gcgcttcctg gcggccatga gccacgagct
     2821 gcgcaccccg ctcaactcca tcctgggctt ctcccagctg ctcctgaccg accgcttcgg
     2881 ctccctgagc cagaagcagg cgaagtacgt ggccaatgtc gagagcagcg gccagcacct
     2941 cctgctcctg atcaacgacc tgctcgacct ctccaaggtc caggcgggca agatggacat
     3001 cgccctttcc gccgtcggcc tgttccccct gatcgaggag tcggtcacca agatggtcga
     3061 actggcggag gcgaaaggcc tcgacctgga ggtcggcgac gacgccggcc tgatcgtcgt
     3121 cgccgacccg ctgcgcctgc gccagattct cctgaacctg ctctccaacg cgatcaagtt
     3181 caccgacaag ggattggtgc ggatcgacgc cgtggcctgc ggcgaggagg tgcgcctgga
     3241 cataaccgac accggtgtcg gcatcgacgc cgaggacctg gagcggatgt tcgaggagtt
     3301 cacccaggcc aagaccgggc tctcgaagtc agcccagggc accggcctcg gccttcccat
     3361 cagccgccgc ctggccgagc tgatgggcgg ccggatcgag gtcgccagca cggtcggcca
     3421 gggcaccacc ttcagcctct tcctgcccgc caccggcgtg ggcgagatcg gcaaggcccc
     3481 gcgccgggtc atctcggcgg ttggctgagg cccgtaaagc ttcccctcag tcgatgaggg
     3541 cctggtacac cagctgcctc agctcggtcc ggatcgtgta ggtgctcgac gggagaagct
     3601 gcgtcatcag gatcacgacc acctcgtcga ccgggtcgac ccagaacgcc gtgctcgccg
     3661 cgccgcccca ggcgagctcg ccgcggttgc tgagcgtctt ggcggcgatc gggtccagca
     3721 cgaccgagaa gccgaggccg aagccgatgc cggtgaagtt cgtctcggcg tagagcggcc
     3781 ggccgaccgt ctccaggtcg tgaccgccgg ggaggtggtt ctgcgccatg aggccgatcg
     3841 tcttcggcgc cagcaggcgg gtgccgtcca gctcgccgcg ccccagcagc agttgcgtga
     3901 agcgatggta gtcggcggtc gtcgagacca ggccaccacc gcccgagaag agtgagggcg
     3961 gcttgagcgc ggcctcgccc atcacgtcgt agcggatcgg cccggggatc ccgggcgcca
     4021 cggcgtagag cgccgccagc cggtcgcgat ccgcctcggg aaccgagaaa tgggtgtcct
     4081 ccatgcccag cggcttgaag atccggtcgg cgaagaagct gtccagcgac tgacctgagg
     4141 ctacctcgat cacccggccc aggacgtccg tcgcgaccga gtagttccac tccgttccag
     4201 gttggaaggc gagcgggatc cgggcccagg tgtcgcaggc cgtggccagg tcggtgccct
     4261 ccggccagcc ccattcgtag cccgcggcac gatacgcctc gtcgacggcg tgggcgtgga
     4321 ggaagccgta ggtcagcccg gcggtgtggg tgagcaggtg ccagatccgg accggctccg
     4381 cgaccggcgc ggtcagcggc ttcgttgagt tgccccggag gtagacccgc gcgtcggcaa
     4441 aggaggggat gaagcggctg accggcgtcg tcagctcgaa cgcgccctgt tcgaagagca
     4501 tcatcgccgc caccgaggtg atgggcttgg tcatcgagta gatccggaag atggtgtccg
     4561 cctcgacggg gaggcccgcc tccttgtcgc gctttccgaa ggaggagaga tgtacgacct
     4621 tgccgcggcg gctgaccata acctgcaggc cgggaagccg gccgtcctcg acgtaggccc
     4681 gcaggtggtc gtcgatccga gacagccggg cggcatcgaa cccgacgctc gccgggtcga
     4741 ccagggtgag ggcagccttc agcgtgtcca ccgtatccac ctctctgaaa aatgcttccc
     4801 agaaacgaaa gtgaccccgg cgggccgcct ctctggctcc tgttcctgcc ttgcggctct
     4861 caccggctcc ctagcgcgca cccatcgggg ccgtcgccaa cactagttgg tcgccgcggc
     4921 cggatgcaac agggatcatt tttcgagttg ccaggccact gctggtagcc gcgccgaatg
     4981 gcctccgtcc gccatcgagg cgccagtgtc ctgcccctga agttcgttgc aaaatcgccg
     5041 ccggccgtcg cttgcgacgg cccagacatt tgttgcgaac tttgggggcg ggacactagg
     5101 gtttccgata tggagctgaa ggacaaggtc gtcatcgtca ccggcggggc cggcggcatc
     5161 gggtccgctc tcggccggcg cttcgctgcc gaaggcgccc gggcggtcgt cctcgccgac
     5221 cgggacggcg cccgcgccga agatgcggcg gcagccatcg ggccggcggc gcgaggcgcc
     5281 gactgtgacg
//
LOCUS       DATCQR010000157        19363 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_8850, whole genome shotgun sequence.
ACCESSION   DATCQR010000157 DATCQR010000000
VERSION     DATCQR010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 19363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19363
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8850"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2273
                     /locus_tag="VIN32_05185"
     CDS             <1..2273
                     /locus_tag="VIN32_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015748849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEY8922006.1"
                     /translation="SGNLPAEATSFIGRRSELAELRKKLTAARLASLVGPGGVGKTRL
                     AIRAGTDLARGFPDGAWLVQLAELEDSKLVGNAVMAALDLRDQAATEPLALLLSYLRD
                     KELLLVVDNCEHVLGAAAQLVTEVIQAAPGVRVIATSREPLSVPGEHVIPLPPLELPH
                     AAQPLGQLRQNEAVMLFTERAAAASGKFELTASNQAAVVDLCRRLDGLPLAIELAAVR
                     TRVLTAEQILERLTDRFGLLTGGSHGAIPRHQTLRTTIEWSHELLAADERTLLRRLCV
                     FAGRFTLEDVEAVCTSDDVPAADGLDLLSSLVDKSLVMKEDAKGLAFYRLHETMREYA
                     GFKLREAGEDEAVQLRFADYYASRCQGSAAQARYGLVEWLEWMDLEIDNVRSVLRRCL
                     AKADFARGIVLASSLGWYWNTRATTEGVRWLDELLASGGGSPVERAQAYFMRGFLAVL
                     QSDPIAARPAIGRAVEAAREAGQLGLLSEALSMASMAENLAGDRANARRFLAESQVIT
                     AGLDDVPVRLMFLQARAFNGLFEGDLDEVRSASSEGARLSREPRDLYSLDTMLMNLGF
                     AALLEGDLAEAKPLHTEALRIAQQIDHRVAQSYLLGALGYRAAASGEPRLAARLLGAA
                     EAIRTEAGTNVVEFFAPFLAQATESAIAALGASRFEAELSAGRGLSRGAAIGLALGES
                     AQVPATPSDVAGHGLLGKREAEVAQLIAEGLSNKQIGARLFISERTVGSHVRSILNKL
                     GFNSRTQIAGWMASDQ"
     gene            complement(2326..2499)
                     /locus_tag="VIN32_05190"
     CDS             complement(2326..2499)
                     /locus_tag="VIN32_05190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8922007.1"
                     /translation="MSMIMGIDRQAVWSYLFGTSDPPESAAVAQERLEHLHWDRGSRS
                     WVAAEVARDQIAA"
     gene            complement(2536..3987)
                     /locus_tag="VIN32_05195"
     CDS             complement(2536..3987)
                     /locus_tag="VIN32_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8922008.1"
                     /translation="MSDRRRWIALVVVCFGQLMIVLDSTIVNVALPPIQRDLHFSQAD
                     LTWVVNAYLITYGSFVLLAGRAGDLVGRKRVFLGGVLLFTVASVLCGIAQDAALLVAG
                     RFLQGIGGALSAGVIIALIVTGFTDPDERARAMSVFTFTIAGGGALGLLAGGLLTQWI
                     NWHWIFFINLPIGVATLLAGRALIEDHEGIGLRQGVDVWGSILVSAALALGVYSIVTS
                     SEFGWASAHTIGFAGVAIALLAAFIWLEARLENPIMPLRILGIRSLTGASAARALLAT
                     GLFTTFFIGALYLQHVRGYSAFGTGLAFLPIPVALAVMSAGVTARLMRRFGAGRILAS
                     GMLAVTAGLVLLAGASEHADYFPRLFVAYALCGLGAGAAMMPLTIIAMAEVPVVDVGL
                     AAGVSNVTMQVAAAIGLAAMGTISTDHSRALAAQGHTLADALTGGYQLAFAIAASCVV
                     VGIVVVLVLLRTPRSAAPSASEERPDEIRAEAA"
     gene            4126..4572
                     /locus_tag="VIN32_05200"
     CDS             4126..4572
                     /locus_tag="VIN32_05200"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY8922009.1"
                     /translation="METEPGPGLIELVSQLHKTFYKRTSEELLGMRMRQYLALCKVRD
                     HAGISQQELAEMMLLDSNAVVLLLNELEDLGYSVRRRDPLDRRRHIVELTRAGKQALV
                     RAEAGRLSMEDELLAGLTAEEKLMLRKILTRALDGLARVPADSAKA"
     gene            4709..5224
                     /locus_tag="VIN32_05205"
     CDS             4709..5224
                     /locus_tag="VIN32_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009945514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system HicB family antitoxin"
                     /protein_id="HEY8922010.1"
                     /translation="MELGPYVDSLRHELLVAAEAGGEDALALAGRLIAPLDSATRLIL
                     LEAMSAAADEITRDLAPGSVEVRLRGRNPSFIVTPPPTEEAFDETTEPRSSALLARTE
                     GDEGGTSRINLRLPDSLKLRAEEAADKEGLSVNAWLVRAVAAALEPDDRDGQARRRSP
                     RGRQSYTGWVR"
     gene            5230..6120
                     /locus_tag="VIN32_05210"
     CDS             5230..6120
                     /locus_tag="VIN32_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HEY8922011.1"
                     /translation="MPTFDTLEPISVTVAIGVGDVRIAASDRSDTVVMVSPSDSSKGS
                     DVKAAEQTQIEYSDGRLLIKAPRSWRRYTPFGGGDSIEVVIELPAGSRVEGEASMADF
                     RCDGRLEECRLTTGVGNIRLDETGPLHLSTGAGSITVDRAVGRADVTGSGQVRIREID
                     GPAVIKNLNGVTWIGEVTGDLRCNAANGDITVDRACAAVAAKTANGAVRIGEVMRGSV
                     EVGTAYGELEVGIREGTAALLDVRSQFGTVRSSLAASDGPEPSDQTVEVRARTSFGDI
                     VIRRSRPLPAVTESHGRVRR"
     gene            6117..7076
                     /locus_tag="VIN32_05215"
     CDS             6117..7076
                     /locus_tag="VIN32_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004562505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY8922012.1"
                     /translation="MTTASSASAIVATGLRKSYGDKVVLDGIDLHIPEGTIFALLGPN
                     GAGKTTAVQILSTYIGADSGEVRVAGHDLARDPDAVRAAIGVTGQFSAVDKLLTGEEN
                     LILMADLHHLGRREGRVRAAELLRRFDLVEAGPNILATYSGGMRRRLDLAMTLIGDPR
                     IIFLDEPTTGLDPRSRHTMWQIIRDLAAGGVTIFLTTQYLEEADQLANRIAVLDDGKV
                     VAEGTADELKRLIPGGHIRLQFADSARLEAAAGILGEVVRDDDALTLQVPSDGGVRSL
                     RALLDRLDHESIEVDGLTLRTADLDDVFFALTGHPTTEKDAAR"
     gene            7073..7849
                     /locus_tag="VIN32_05220"
     CDS             7073..7849
                     /locus_tag="VIN32_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015103816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8922013.1"
                     /translation="MSIYSLSDSATMLRRDFRHLRRYPVMTISGVVTPTVMLLLFVYV
                     FGGAMSSGLGGAGASYVNYLVPGILMMTLGSGCAATAVGVNMDMTEGIVARFRTMAIS
                     RASVLTGRVAGSMIRTLISVVLVIGVALIAGFRPTAGPVQWLEAIGFITLLTLALTWL
                     AIGLGMAAKSPEGANGSTLLLQFGPFISSGFVRPDTMPAGVRWFAENQPFTSMIETMR
                     GLLLGTPIGNSAIMATAWCIAIASVGYLWARATYKRDPIR"
     gene            complement(8123..8497)
                     /locus_tag="VIN32_05225"
     CDS             complement(8123..8497)
                     /locus_tag="VIN32_05225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8922014.1"
                     /translation="MLLLGILLVVGSTLSLFATAIFPFDSFAGTDTSVAGVAFGVGIA
                     VASFNPGAQIGWVRAAILYMILLVVYRIVFGLFWGTWGQLAPLVIAIVFGVALIILFP
                     RRGELMPHQGSVADGPLPQSVR"
     gene            complement(8599..9777)
                     /locus_tag="VIN32_05230"
     CDS             complement(8599..9777)
                     /locus_tag="VIN32_05230"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HEY8922015.1"
                     /translation="MAAIFAVRDLVLAVGEDSRPDALALHVAAVRYLYEPAALYAEAA
                     QALAGTGTLPVPGHGFLYPATAAWLGIPFAALPASVAIVAWTVADAAAMVAALVILYR
                     LVRPAGLGRPVFWLVAAYFPPLFAEVDAGQVGGVLLLLTAGASWALVRRPALAGALVG
                     FAAAIKYYPAAWLLGVWRWRPWVAAAVTGALALAAGFARLGPRGAVYYVSSVLLPALH
                     STYADCAIVSIHTLSARLFADETFFVPGNAGIRAVNAGLNLPWLAQPVTYLIDAAVVL
                     AVLWATRRSRTHPLYAPLLALSLGALLPSEINPYQMLPLLPITVLVMVRAIEMRRPGL
                     IAMGVLGLALFVRQPCDSPFPNLWTIGALVLFAAAVAAAPLFVEAAESAEDRGTRFAE
                     "
     gene            complement(9874..11100)
                     /locus_tag="VIN32_05235"
     CDS             complement(9874..11100)
                     /locus_tag="VIN32_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012894994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY8922016.1"
                     /translation="MTKHGKQGEPAELELTVLLPCLNEERTIGACIAEARDAIAEYGL
                     AAEVLVADNGSEDASIQVAHAAGARVVKVSGRGYGNVLRIGIAAAHGRYVIMADADGS
                     YDLGQLLPFLERLRLGDQLVMGDRFRGGIAPGAMPWLHRWVGNPILSWLGRLFFSARI
                     ADFHCGMRGFDRRALLSLELHTSGMEFASEMVICAALDGFRVANVPVKLRRDGRDRRP
                     HLRTWRDGWRHLRFMLVLAPSWLFLYPGLLLMAVGGVVGGLLVAGPIYLGRIQLDIDT
                     LLVMAGAVLLGYQLVGFASFAEAFAIGGGIRGGRRRAGPFEWLKLEVGIVLGLGLMVA
                     GTAGIAVEAGGWAALGYGQLNPEQSFRVLIPSLLALSMGFQTFFGSFFLDLLRMPIRV
                     KRRAADAAKQLADLRR"
     gene            complement(11147..11488)
                     /locus_tag="VIN32_05240"
     CDS             complement(11147..11488)
                     /locus_tag="VIN32_05240"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEY8922017.1"
                     /translation="MINRRRNRRQFPRLDPPAGLRPAAAEVFHRFLDISIGGLRVLSR
                     ANWKTGDMIQLEIRFPDSTDLIVTAEVVWIRRLSLAAEITNEAGLRFTDLTDFDRSRI
                     AALLAARSHPQ"
     gene            complement(11591..13018)
                     /locus_tag="VIN32_05245"
     CDS             complement(11591..13018)
                     /locus_tag="VIN32_05245"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY8922018.1"
                     /translation="MGELRDVWYRRNRHYHRYLESQLRAIIPAGSSVLELGCATGNLL
                     NVLKPARGLGIDLSPAMVGAAAHKFPHLEFRVGNAEALVLDERFDYVVVSDVIGELSD
                     VAAMLERIHDLSREGARLVITFHNPALETVIRLAQRLGLALTPARQNWISPLVMSSML
                     ELADYELVSMSAGFLFPFRLPVVAGFVNERLTRIPGLRYLNLLNVAVAKPLRPRPVVA
                     PMSCSVVVPCRNEVANIEAAVERLPDVGVSTEIIFVDGDSSDGTQRRILEMIQFYRGR
                     KNIRLIHQLPDTAGGPAQEAPGSMLKLGKGDAVRKAFDAATGDVLIILDADLTVPPEA
                     LPAFHNALASGKARFVNGSRLVYPMEDRAMRLANYFGNKFFAVLFSWLLDQPVRDTLC
                     GTKALLRSDYELIRAGRTHFGDFDPFGDFDLLFGAARLRLRILEIPVRYRRRLSGVSK
                     VSVLRHGWLLVAMAWVGFWKLKVSR"
     gene            complement(13080..14108)
                     /locus_tag="VIN32_05250"
     CDS             complement(13080..14108)
                     /locus_tag="VIN32_05250"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY8922019.1"
                     /translation="MQQHHRRRRGKQTEQGRQHERPPELTRSKRGQQAGSVSPASQRV
                     SEQALANTAPPDRNQPTCPLCGARVSGRVGRWNELAVWRCEGCQHSFVAPVPDPTQLA
                     AIYEAGYYEGSTQPTGFRNYAGLHDARIRMFGRHLDLIAKGGGAGRLLDVGCANGDFL
                     VEARHRGWDVLGVDPSAARTEVIAAGVPLVGTTVRDAAVAERSLDVITFWDVLEHVSD
                     PVEDLRSAKRLLKVGGLVVATVPDAGNVVANLSGRRWFGFRTTGEHLQFFSRQSLRKA
                     MEEAGFRVISQHGVPWSCTLGFLGDRAGLYLGVPGLLMRRALERPRFKHQIVDVPLIN
                     QLALCVAD"
     gene            14094..15113
                     /locus_tag="VIN32_05255"
     CDS             14094..15113
                     /locus_tag="VIN32_05255"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HEY8922020.1"
                     /translation="MVLLQAVFISNGHSPWGFEAGQAWSIDRDLMRGIRYENSPWSPT
                     YPLPSELFFLPLALLSQEAAQVISIVFTAVLIAGALVLWNQTSHAPWTSLLPLFISVP
                     VLGAVQIDHPYSAVGLAGLGLAAWAYRRQSWLLLGVGAALALIRPQNALAALPVVLIG
                     LSGRAAIRTGMGAAAVLVPLVAASFLWDPGWFGAYQEDVRLYPSSGIYGLLVRSIGVV
                     GISAILLALGVGGLWLRRLGHDPLDAFTLVLAATVLVVPDPGLYCGLFVLPALQRIGA
                     RPEYRAVPWVASASAWAIPLAVSPWLLANPPAIFPLLSALAYWLAIQTYPLLRRGHKP
                     QDGGP"
     gene            complement(15652..16155)
                     /locus_tag="VIN32_05260"
     CDS             complement(15652..16155)
                     /locus_tag="VIN32_05260"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY8922021.1"
                     /translation="MAKDWTDTLLDRWTADRPRQDMGAYQVTARISRIGLHIAQQQEG
                     AFSRFGLNRGEVGVLAALRFAGAKEQLSPTQLFKGLMLSSAGITSRLDRLERRGYVKR
                     SRSPDDRRGVLVELTDAGREVLDQAVDANTGRENEMLASLSKEEQDALAGLLKKLLAG
                     LEPSQGD"
     gene            16433..16798
                     /locus_tag="VIN32_05265"
     CDS             16433..16798
                     /locus_tag="VIN32_05265"
                     /inference="COORDINATES: protein motif:HMM:NF015596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8922022.1"
                     /translation="MPGTTIDVGSTRLGQVLVDGAGLTLYLFLADSGTNSTCNSSACV
                     QYWPPVLTTGAPQAGTGVSASLLGTTARQDGTTEVTYAGHPLYYFISDKKAGDVSGQG
                     INGFGAPWYVVAPSGMRIG"
     gene            17117..17893
                     /gene="fabG"
                     /locus_tag="VIN32_05270"
     CDS             17117..17893
                     /gene="fabG"
                     /locus_tag="VIN32_05270"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009742534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HEY8922023.1"
                     /translation="MLQPIAGRVAVVTGGSRGIGKGIARTLARHGVRVLIASRGEDAA
                     RGVVDEIRSAGGNAEYVTADVSAPDDAPRIAEAALEHFGGIDILCSNAGIFPPRSLRE
                     LTAAAIDEVFNVNLRGTMLVVKACLPALERSGHGRVILTSSITGPVTGYPGWSHYGAS
                     KAGQLGFMRTVAMEVASAGITVNAVLPGNIKTEGLADLGEEYERTMAAAIPLRRLGTV
                     DDVANTVVFLASDEAAYLTGVAIVVDGGQILPESLAALEG"
     gene            17921..18049
                     /locus_tag="VIN32_05275"
     CDS             17921..18049
                     /locus_tag="VIN32_05275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8922024.1"
                     /translation="MPVIRIAAAQFSVSGDKADNPAVVLDPRRGLRSYVEIAARIV"
     gene            18243..>19363
                     /locus_tag="VIN32_05280"
     CDS             18243..>19363
                     /locus_tag="VIN32_05280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8922025.1"
                     /translation="MVESKWRRLRTIPVALLLALSSAGSAFTPVMAATSVTQVDFTQC
                     ANDPVPSAATNCPGGWINGILQGSNSHYAEDLVVPQRIVVDVPGTLTGATTHTLTVRY
                     QARKASTHAYDSIATWNYTQTGADRCQSLATGTCPTGSASTFAIPFDCTEVPAATLPG
                     TAQGGCSSSANDVTARHQLAGQVATMYGGALTDITAPVHDSAAAPGTDDYATVTFSYT
                     VAASTAADNYVELLFGGHLAAGLRSRGWGVGLGASSINGGPYHFKWDLSDGASIGNRD
                     NQISAGGIMAAPTVSTLLSASSVGAGTPVTDSATLIGAASNAGGTVTYNVFAGNVCTG
                     TPVFTSTVTVTGAVVPPSAPFNTALAPAAPGSYQWQASY"
BASE COUNT         3458 a         6386 c         6350 g         3169 t
ORIGIN      
        1 gctcgggcaa cctgccggct gaggcgacca gcttcatcgg gcgacgcagc gagcttgcgg
       61 aactgaggaa gaagctcacc gcggctcgcc tggccagcct cgtcggaccg ggcggcgtcg
      121 gcaaaacccg cctcgccatc cgggccggga cagacctcgc ccgtggcttc ccggacggag
      181 cctggttggt ccagctggct gaactggagg attccaaact ggtgggcaac gccgtgatgg
      241 cggccctgga cctgcgggac caggcggcga ccgagccgct ggccctcctc ctctcctacc
      301 tgcgggacaa ggagctgttg ctggtggtcg ataactgtga gcacgtgctc ggagcagccg
      361 ctcagctcgt caccgaagtc atccaggcgg cgccgggtgt gcgtgtgatc gccaccagcc
      421 gcgagccgct gtcggtgccg ggcgagcatg tcatcccact cccacccctc gagctgccgc
      481 acgcagccca gcccctcggc caactcagac agaacgaagc ggtgatgttg ttcacggaga
      541 gagcggcggc ggcatcgggc aagttcgagc tgaccgcctc gaaccaggcg gccgtcgtcg
      601 atctctgccg ccggctcgat ggtctgccct tggcgatcga gctcgctgcg gtcagaacgc
      661 gagtccttac cgcggagcag atcctcgagc gtctgaccga ccggttcggc ctgctcacgg
      721 gaggaagcca cggtgccatc ccacgtcacc agacgctccg aaccaccatt gaatggagtc
      781 acgagctgct ggcggcagat gagcgaacgc tcctcaggcg gttatgtgtg ttcgcaggcc
      841 gcttcacgct ggaggacgtc gaggcggttt gcacttcgga cgacgtgcct gcagcagatg
      901 ggctcgacct gttgtcatcg ctggtcgaca agtcgctggt gatgaaggag gacgcgaagg
      961 gcctcgcctt ctatcgcctg cacgagacga tgagggagta tgccggcttc aagctgcgcg
     1021 aggccggtga ggacgaagcc gtccagctgc gcttcgccga ctactacgcg tcccgctgtc
     1081 aagggtctgc ggcgcaagct cggtatgggc ttgtcgagtg gctggaatgg atggacctgg
     1141 agatcgacaa cgtccgatca gtccttcggc gctgcctggc taaggccgac tttgcacgcg
     1201 gaatcgtcct cgccagctct ctgggttggt actggaacac tcgcgcaacc acggaaggag
     1261 tgcggtggct ggatgagctg ctcgcctctg ggggtggtag cccggtagag cgtgcccagg
     1321 cttacttcat gcgtggattc ctggccgtgt tgcagtctga tccgatagct gccaggccgg
     1381 ccattggacg agcagtggag gcggcccggg aggcggggca gctcggactg ctgtcggaag
     1441 cgctgtcgat ggcctcgatg gccgagaacc tggccggtga tcgcgctaac gccaggcgtt
     1501 tccttgctga atctcaagtc atcacggctg gtcttgatga cgttcccgtg aggctcatgt
     1561 tcctccaggc ccgggcgttc aacggtctct tcgagggcga tctggacgag gtaagatccg
     1621 cgtcctcaga gggtgcgcgt ctcagccgag aaccgcgtga cctctactcg cttgacacga
     1681 tgctcatgaa cctgggcttc gccgcgctgc tcgagggcga tcttgccgag gcaaagcctc
     1741 tgcacacgga agctctgcgt atcgctcagc agatcgatca ccgcgtcgcg cagtcctact
     1801 tgctcggcgc cctgggctat cgtgcggcgg cgtcgggcga gccgcgactg gcggcgcggc
     1861 tgctcggcgc cgccgaggcc atccgaaccg aggccggcac aaacgtcgtt gaattctttg
     1921 ctccatttct ggcccaggcc acggaatccg cgattgcggc acttggggct tcgaggttcg
     1981 aggcggagct gagcgcagga agaggcctga gtcgaggcgc cgcaatcggc ctcgcgcttg
     2041 gtgaatccgc tcaggtacca gccacgccct cagacgttgc gggacatggg ctgctcggga
     2101 agcgcgaagc ggaggtcgcc caattgatcg ccgagggctt gagcaacaag cagatcggag
     2161 cgcgattgtt catatccgag cgcaccgtgg gcagccacgt ccgcagcatc ctgaacaagc
     2221 tcggcttcaa ctcgcgcacc cagattgccg gctggatggc gtcggatcag tagccgacga
     2281 ggggtccgac gcggccccga agaacacggc gggctgtgga ccgcctcagg ccgcgatctg
     2341 gtcccttgct acctcggcgg cgacccagct gcgggagccg cgatcccagt gcaggtgctc
     2401 cagcctctcc tgcgcgacag ctgcgctctc gggtggatcg ctggttccga agaggtagct
     2461 ccagacagcc tgccgatcga ttcccatgat cattgacatc ggaaacctcc tttgacgatt
     2521 cctggattcc attggctacg cggcctcggc gcggatctca tccggccgct cctcggacgc
     2581 ggatggggcg gcgctgcggg gtgtacgcag gaggaccagc accacgacga tgccgaccac
     2641 gacgcatgac gcggcgattg cgaaggcgag ctggtagccg ccggtgagcg cgtcggcaag
     2701 ggtgtggccc tgcgcggcaa gggcccggct gtggtcggtt gagatggtgc ccatcgctgc
     2761 gaggccgatc gccgcggcca cctgcatggt gacgttgctg acgccggcgg caagtccgac
     2821 atcgacaacc ggaacctcgg ccatggcaat gatcgtgagc ggcatcatgg cggcgccggc
     2881 gccaagcccg cagagcgcat aggcgacgaa cagcctcggg aaatagtcgg cgtgctcgct
     2941 cgctcccgcc agcaacacca gccctgcggt gaccgcgagc atgcccgagg cgaggatccg
     3001 gccggcgccg aagcgcctca tcagccgggc ggtgacaccg gccgacatca cggccagcgc
     3061 caccgggatg ggcaggaagg cgagaccggt gccgaaagca ctgtagccgc ggacatgctg
     3121 caggtagagg gcgccgatga agaaggtcgt gaacaacccg gtcgcgagca gcgcgcgggc
     3181 ggcgctcgcg cccgtgagac tgcggatgcc aaggatgcgc agcggcatga tcgggttttc
     3241 gagccgcgcc tcgagccaga tgaacgccgc cagcagggca atcgctacgc ccgcgaagcc
     3301 gatcgtgtgc gccgaggccc agccgaattc ggacgaggtc acgatcgagt agaccccgag
     3361 cgccagtgcg gcgctgacca ggatcgagcc ccagacatcg actccctggc ggaggccgat
     3421 gccttcgtga tcctcgatca acgcgcgccc cgcgagcaag gtcgccacgc cgatcggcag
     3481 gttgatgaag aagatccagt gccagttgat ccactgggtg agcagcccac cggccaggag
     3541 accgagggcg ccgccgcccg cgatcgtgaa ggtgaagacg ctcatcgccc gcgcgcgttc
     3601 gtcgggatcg gtgaagccgg tgacgatgag ggcgatgatc acaccggcgg agagggcgcc
     3661 gccgatgccc tgcaggaagc ggcccgccac caggagggct gcatcctgcg cgatcccgca
     3721 gagcaccgat gccacggtga acaggaggac cccgcccagg aagaccctct tgcggcccac
     3781 caggtcgccg gcgcggccgg ccagaagcac gaagctgcca taggtgatca ggtacgcgtt
     3841 cacgacccag gtcaggtcgg cctgactgaa gtggaggtcg cgctggatcg gcggcagggc
     3901 gacattcacg atcgtcgagt cgagcacgat catcagctgg ccaaagcaga cgaccacgag
     3961 agcgatccac cgccggcgat cgctcaatct gtcctgtatg gtcaataggt tcgggatcat
     4021 gcctgccctc cattcgctac ggccatcgtg atgatcacta tagcatggat tatcgcaaaa
     4081 tgttaatgat ccgatggacc gtggtaggat ttagttcgaa agcacatgga gaccgagccc
     4141 ggaccgggac tgatcgagct ggtgagccag ctccacaaga ccttttacaa gcgcacctcc
     4201 gaagagctgc tgggcatgcg gatgcgccag tacctggcgc tctgcaaggt ccgcgaccat
     4261 gccggcatct cccagcagga actggccgag atgatgcttc tggactccaa cgcggtggtt
     4321 ctcctcctca acgagctgga ggacctcggc tactcggtgc ggcggcgcga tccgctcgac
     4381 cggcgccggc acatcgtcga gctcacgcgg gccggtaagc aggcgctggt gcgcgccgag
     4441 gccggccgcc tcagcatgga ggatgagctc ctggcgggcc tcaccgcgga ggagaagctg
     4501 atgctgcgga agatcctgac ccgggcgctg gacgggctgg cccgcgtgcc ggcagattcg
     4561 gccaaggcct agcatccagc cgggcggcgg cccgcggagg tccgccacga atataaatcg
     4621 agccttttgg ggttcgcctg tttccctgac ataggctgag ccatcacggc acttgacggg
     4681 gagccattat ggtgccatag tggctcaggt ggaactcggc ccttacgtcg acagccttcg
     4741 gcacgagctg ttggtagccg cagaagccgg cggcgaggac gctctcgcac ttgccgggcg
     4801 gctgatcgca ccgctcgact cggcgacccg gctaatcctg ctggaggcca tgtcggcagc
     4861 cgcggacgag atcacccgcg atctcgctcc tggctcggtc gaggtgcgcc tgcgcggacg
     4921 gaaccccagt ttcatcgtga cgccgccgcc gaccgaggag gcgttcgacg agacgacgga
     4981 accgcggtcg tccgctctct tggcccggac cgagggcgac gagggcggga cgtcgcggat
     5041 caatctccga cttccggatt ccctcaagct ccgagccgag gaggccgccg acaaggaagg
     5101 cctctcggtc aatgcgtggc tcgttcgggc cgtggccgcc gcgctggagc ctgacgaccg
     5161 cgacggccaa gctcggcgcc gcagcccgcg aggccgacag agctacaccg gctgggtgcg
     5221 ctagcggcca tgccaacgtt cgacaccctt gagccgatct ctgtgacagt cgccataggc
     5281 gtcggcgacg tgcggatcgc cgcgagcgac cgcagcgaca ccgtcgtcat ggtcagcccg
     5341 agcgacagct ccaaggggtc cgacgtgaag gccgccgagc agacacagat cgagtactcc
     5401 gacggcaggc tcctgattaa ggcaccaagg tcatggaggc gttacacccc attcggtggt
     5461 ggcgactcca tcgaagtggt gatcgagctg ccggcgggat cgcgcgtcga gggcgaagct
     5521 tcgatggcgg acttccgctg cgatggtcgc ctcgaagagt gccggctaac caccggggtc
     5581 ggcaacatcc ggctcgacga gacgggtccg ctccacttga gcaccggtgc cggcagcatc
     5641 accgtcgacc gtgccgtggg ccgggctgat gtcaccggat ccggccaggt gcgcatccgc
     5701 gagatcgacg gtcctgcggt gatcaagaat ctcaacggcg tcacctggat cggcgaggtc
     5761 accggcgacc tgcgttgcaa cgccgccaac ggcgacatca ccgtcgaccg ggcatgcgcg
     5821 gcggtagccg ccaagaccgc caacggcgcc gtccggatcg gcgaggtaat gcgcggctcg
     5881 gtcgaggtgg ggaccgccta cggtgagctc gaggtaggca tccgggaggg caccgctgcc
     5941 ttgctcgacg tgcgctccca gttcggaacc gtgcgcagct cgctggcggc ctccgacggc
     6001 cccgagccgt ccgaccagac cgtcgaggtg cgcgcgcgca cctccttcgg ggacatcgtg
     6061 atccgccgat cgcgaccgct tcccgcggtg acggagagcc acgggagagt acgacgatga
     6121 ccaccgcctc atccgcgtcg gcgatcgtgg cgacggggct cagaaagtcg tacggcgaca
     6181 aggtcgtgct cgacggcatc gacctgcaca ttcccgaggg aacgatcttt gcgctgctcg
     6241 ggcccaacgg ggccggaaag accaccgcgg tgcagatcct gtcgacgtac atcggcgccg
     6301 acagtggcga ggttcgagtc gccggtcacg atctggcccg ggaccctgat gcggtacggg
     6361 ccgcgatcgg cgtcaccggc cagttctcag cagtcgacaa gctcctcacg ggcgaggaga
     6421 acctgatcct gatggcggat ctgcaccacc tgggccggcg tgaaggccga gtgcgcgccg
     6481 ccgagttgct ccggcgattc gacctggtgg aggcgggccc gaatatcctg gcgacctact
     6541 caggtgggat gcgacgccga ctcgacctcg cgatgaccct gatcggtgac ccgcgcatca
     6601 tcttcctcga cgagccgaca accggcctcg acccgcgcag ccggcacacg atgtggcaga
     6661 tcatccgcga cctggcggcc ggcggggtca ctatcttcct gaccacccag tacctggagg
     6721 aggccgacca gctcgccaac cggatcgcgg tcctcgacga cgggaaggtg gtcgccgagg
     6781 gcaccgcgga cgagctcaag cgcctcattc ccggcggcca tatccggctg caatttgccg
     6841 actccgcgag gttggaggca gccgccggca tcctcgggga ggtggtgcgc gacgacgacg
     6901 cgctgaccct gcaggtccca agcgacggcg gcgtccggtc gcttcgggca ctgctcgacc
     6961 ggctcgacca cgaaagcatc gaggtcgacg gactgaccct acgcacggca gatctcgatg
     7021 acgtcttctt cgccctgacc ggccacccca ccaccgagaa ggacgccgct cgatgagcat
     7081 ctactcattg agtgattccg caaccatgct gcgccgcgat ttccggcacc tgcggcgcta
     7141 ccccgtcatg acgatcagcg gggtcgtgac gccgactgtc atgctgctgc tgttcgtcta
     7201 cgtctttggc ggtgcgatga gctctggcct cggcggcgct ggtgcttcat acgtcaacta
     7261 tctcgtcccg gggatcctca tgatgaccct cggcagtggc tgcgcggcga cggccgtggg
     7321 cgtcaatatg gacatgaccg aaggcatcgt cgcccgcttc cgcaccatgg ctatctctcg
     7381 ggcttcggtt ctgaccgggc gcgtggccgg cagcatgatc aggacgctga tcagcgtggt
     7441 cctcgtcatc ggtgtcgctc tgatcgccgg ctttcgaccc accgccggcc cggttcaatg
     7501 gctggaggcg atcggcttca tcacgctgct caccctcgca ttgacctggc tggccatcgg
     7561 gttgggtatg gcggccaagt cacccgaagg agccaacggc tcgacgcttc tcctccagtt
     7621 cgggcccttc atcagcagtg gcttcgtgcg cccggacacg atgccggcgg gggtgcgctg
     7681 gttcgccgag aaccaaccct tcacctcgat gatcgagacc atgcgaggac tgctgctcgg
     7741 gactccgatc ggcaacagtg caatcatggc caccgcgtgg tgcatcgcga tcgcatcggt
     7801 cggatacctc tgggcgcggg cgacatataa gcgcgacccc atccgctaat cactcggaag
     7861 tcttctacct ggtgaccggt gccaccctcg ccgccggcgg atcccgcatc aactcattct
     7921 gtcagcgctg cggtggccgg atcgccgtcg ggatgtaccc ctagaccgaa acaccgagag
     7981 cgcaaccatc cagccacggt cccttgtaat gcgtcaagca cgacgcgatg ccaaacggcg
     8041 acagcttggc gcggggctgg gtctggatac tcgggcaaat gagggaagaa cccaatggct
     8101 cttccgtcgc cagttacttc cgttaacgca cggattgggg aagtggcccg tcagcgaccg
     8161 acccctggtg aggcatcaac tcgccgcggc gagggaagag gatgataagc gcgacgccga
     8221 agacgatcgc gatgaccagc ggggcgagct ggccccaggt gccccagaac aatccgaaca
     8281 cgatccgata gacgaccaga aggatcatgt agaggatcgc ggccctaacc cagccgatct
     8341 gggcaccagg gttgaaggaa gcgacagcaa tgccgacgcc gaatgccacg ccggccaccg
     8401 aggtatcagt gcccgcgaag ctgtcgaacg gaaaaatagc cgtcgcgaag agcgaaagcg
     8461 tgcttccgac gaccaggaga atgcccagca ggagcattac gacggtcagt agtcgggccc
     8521 tcaacatcaa gctcatcgat cggtgcctcc tttgcttgtc gggatctgcg gccggaagcc
     8581 gcatgaagtc acacgcggtt actccgcgaa ccttgtacca cgatcttcgg cgctttccgc
     8641 cgcttcgaca aacaatggcg cggcggccac tgcagctgca aagagaacca gggctccgat
     8701 tgtccacaaa ttggggaacg ggctatcgca gggctgccgc acgaatagcg ccagaccgag
     8761 cacgcccatg gcgatcagcc caggccgccg catctcgatc gcgcggacca tgacgagcac
     8821 cgttatcggt agcagcggga gcatctggta ggggttgatc tcactcggaa ggagggcgcc
     8881 gaggctcagc gccaacaggg gtgcgtagag cggatgggtg cgagagcgcc gcgtcgccca
     8941 gagcacggct aggacgacgg cggcgtcgat gaggtaggtc accggctggg ccagccaagg
     9001 gaggttcagg ccagcgttca cggcacggat accggcgttg ccaggcacga agaaggtctc
     9061 gtccgcaaac agtcgggcgg acaaggtatg tatggagacg atcgcgcagt cggcgtaggt
     9121 ggagtgcagt gcaggtagca acaccgagct gacgtaatag acggctccac gcggtcccag
     9181 ccgcgcaaat cccgcagcga gcgcaagcgc gcctgtcacc gcggctgcaa cccaggggcg
     9241 ccaccgccaa actcccagca gccaggcggc ggggtagtac ttgatggccg cggcgaatcc
     9301 caccagggcg ccggcaagcg ccggcctgcg taccagcgcc cagctcgcgc ctgccgtcag
     9361 cagcagaagc acgcccccaa cctgccctgc gtctacctct gcgaacagag gtgggaaata
     9421 ggccgccacc aaccagaaga ccgggcggcc caggccggcc gggcgcacca ggcggtagag
     9481 gatgaccagc gccgcgacca tcgccgccgc gtcggccacc gtccaggcga cgatggcaac
     9541 gctggctggg agagcagcaa acgggatgcc cagccaagcg gcagtcgccg ggtacaggaa
     9601 gccgtggccc gggacaggga gcgtgcccgt tcctgctaag gcctgggcgg cttccgcata
     9661 gagcgccgcg ggctcgtaca agtacctgac tgcggcaacg tggagcgcga gcgcgtccgg
     9721 ccgggaatct tcgccgaccg cgagcaccag gtcgcggaca gcgaagatcg cagccattgc
     9781 ccaccagagc cccggctcgc caagccggcg taagagcccc gctgccgaga ccgccggcgt
     9841 tcgggcagtc ccgtggtttt ccggcccggt cacctaccgc cgcaggtcgg cgagttgctt
     9901 ggcggcgtca gcagctcgac gcttgacccg gatcggcatc cggagcagat cgagaaagaa
     9961 gctcccgaaa aacgtctgga agcccattga gagagccagc aggcttggta tcagcacccg
    10021 gaagctctgc tcgggattga gctgcccata cccaagagcc gcccaaccgc cggcttcgac
    10081 cgcgatgccg gcggttccgg cgaccatcag gccaagcccg aggactatgc cgacttcgag
    10141 tttgagccac tcgaaaggcc ccgcacgccg acggccgcca cggatgcctc cgccgatggc
    10201 gaatgcctcg gcgaaactag cgaagcccac caactggtag cccaagagca cggcgccggc
    10261 catgaccagc agggtatcga tgtcgagttg aatccggccc aggtaaatgg ggcccgcgac
    10321 gaggagcccg ccgaccacgc cgccgacagc catcagtagg agtcccgggt agaggaagag
    10381 ccagctcggg gcgagaacca gcatgaagcg gaggtgccgc cagccgtcgc gccatgtccg
    10441 caggtggggt cggcgatcgc gcccatcgcg acgcagcttg accgggacgt tggcgacccg
    10501 gaatccatcc agcgcagcac agatcaccat ctcgctggcg aactccatac cggaggtgtg
    10561 aagctcgagg gacagcagcg cacgcctgtc gaagccgcgc attccacagt ggaagtccgc
    10621 gatccgcgcc gagaagaaaa gccgtccgag ccagctcagg attggattgc ctacccagcg
    10681 gtgcagccat ggcattgcgc ctggcgcgat gccgccccgg aagcggtccc ccatgaccag
    10741 ttggtcgccc aggcggagac gttccaaaaa ggggagtaac tggccgaggt cgtaactccc
    10801 atccgcgtcg gccatgatca cgtacctgcc gtgggcggcc gcgatcccaa ttctgagcac
    10861 gttgccgtac ccgcggcctg agaccttgac caccctggca ccagcggcgt gggcgacctg
    10921 gatcgaggcg tcctcgctgc cgttgtcggc gaccagcacc tcggcggcga ggccgtactc
    10981 cgcgatcgca tcgcgcgctt cggcaatgca ggcaccgatt gtccgctctt cgttcaggca
    11041 aggaaggagg acggtcagct ccagctcagc gggttcgcct tgctttccgt gcttggtcat
    11101 gggggtcgac gtactcgtca atgctcgggc catgcagaat tctggttcat tgcggatggc
    11161 tccgtgcggc cagcagcgcg gcgatgcggg aacggtcgaa gtcggtcagg tcggtgaacc
    11221 ggagtccagc ctcgttggtg atttccgccg caagcgaaag cctgcggatc cataccacct
    11281 ccgcggtcac aatcaagtca gtactgtccg ggaaccggat ctcgagctgg atcatatcgc
    11341 ccgttttcca gtttgcccgg gagagcacgc gcaggccgcc gatgctgatg tcgaggaagc
    11401 gatgaaatac ctcggctgcc gccggcctga gaccggccgg ggggtcaagg cggggaaatt
    11461 ggcgccgatt gcgccgacgg ttaatcatgg ggagaactca cggacctgga tccccgtcgc
    11521 cgcccgcgct tcgatggcgg gcggccgatt ttggtcttgt accgagagcc aggcaccgga
    11581 agtccatggt tcagcggctg accttcagct tccagaagcc cacccacgcc atcgccacca
    11641 gcaaccaacc gtggcgaagc acactgacct tcgaaacccc cgacaaccgg cgccggtagc
    11701 ggacggggat ctcgaggatg cgaagtcgga gacgggccgc gccgaacagc aggtcgaagt
    11761 ctccgaaggg atcgaagtct ccgaagtgtg tgcgtccggc cctaattagc tcgtagtcgc
    11821 tccggagcaa ggcctttgtg ccgcacagcg tgtcgcgcac cggctgatcg agtagccatg
    11881 agaaaaggac agcgaagaac ttgtttccga agtagttggc gagccgcatt gcccggtcct
    11941 ccatcgggta gacaagccga ctgccattga cgaaccgcgc tttgccgctt gccagggcgt
    12001 tgtggaaagc gggcagcgcc tccggcggaa cggtgagatc tgcgtcgagg atgatcagca
    12061 cgtcgcccgt ggccgcatcg aaggccttgc gcacggcgtc tcctttgccg agcttcagca
    12121 tagaaccggg cgcctcctgt gccggcccgc ctgcagtgtc gggtagctgg tggatcaggc
    12181 ggatgttctt ccggccacgg tagaactgaa tcatctcgag gatgcgccgc tgagtaccgt
    12241 cactcgagtc gccgtcgacg aagatgatct cggttgaaac acccacgtcg ggtagccgct
    12301 ccacagccgc ctcgatattg gcgacctcat tccgacaggg caccaccacc gagcaactca
    12361 tcggcgcaac gactggtcgg ggccggaggg gcttggcgac cgcaacgttg aggaggttca
    12421 gatagcgcag ccccggtatt cgtgtaagac gctcgttaac gaacccagcg acgaccggca
    12481 ggcgaaaagg aaacaggaaa cccgccgaca tgctcaccag ctcgtagtcg gcaagctcca
    12541 acatcgacga catcaccagc gggctgatcc agttctggcg cgcgggcgtc agggctaggc
    12601 ccaaccgttg ggccagccgg atcacggttt caagagcggg gttgtggaat gtgattacca
    12661 acctcgcgcc ctcacgactc aagtcatgga tccgctccag catggccgcc acatcgctca
    12721 gctccccgat gacgtcggag acgactacgt agtcgaatct ctcatcaagg accagggcct
    12781 ctgcattgcc gacccggaac tccaggtgtg gaaacttgtg tgcggcggct cccaccatgg
    12841 ccgggctcag gtcgatcccg agcccacggg cgggcttcag cacattcaga agattgccgg
    12901 tggcacagcc gagctcgaga acggagctgc cggccgggat gatcgctcgt agctgagact
    12961 cgaggtagcg atggtaatga cgattgcgtc gataccaaac atcgcgcagc tcgcccattt
    13021 ggttgtagtg ctctcgcaca gcgtccgtga gagggtcacc aatggccgct accacggatt
    13081 cagtccgcga cgcatagcgc caactgattg atcagcggga cgtccacgat ctggtgcttg
    13141 aaccgcggtc gttccagcgc tcggcgcatg agcagccccg gcacgccgag gtacagcccc
    13201 gccctgtcgc ccagaaaacc cagcgtgcag ctccagggaa caccgtgctg cgagatgacc
    13261 cggaaacccg cctcctccat ggccttcctg aggctctggc gcgaaaagaa ctggaggtgc
    13321 tcgccggtgg tgcggaagcc gaaccaacgc cggccgctca ggttcgcaac gacgttgcct
    13381 gcatcgggaa cggtggcgac gaccagtcct cccactttga gcaagcgctt cgccgatcga
    13441 aggtcctcga ccgggtcgga cacgtgctcc agcacgtccc aaaaggtgat cacgtcgagc
    13501 gatcgctccg ccaccgcggc gtccctcaca gttgttccaa cgagcggcac gccggcagcg
    13561 atcacctcgg tccgagcggc tgacgggtcg acgcccagga catcccagcc gcgatgccgc
    13621 gcctccacca ggaaatcgcc gttcgcgcat ccgacatcga gtagccgtcc cgctccgcca
    13681 cccttggcga tgagatccag gtgccgacca aacatccgga tgcgcgcgtc atgcaggccg
    13741 gcgtagtttc gaaagccggt cggctgggtc gaaccctcgt agtagccggc ctcgtagatc
    13801 gcagcgagtt gggtgggatc ggggactggt gcgacgaagc tgtgctggca cccctcgcag
    13861 cgccagacgg cgagttcgtt ccagcgaccg acgcggccgg agactcgtgc gccgcacagc
    13921 ggacaggtcg gctggttgcg atcgggcggc gcggtgttgg cgagcgcctg ttcagacacc
    13981 cgctgcgacg ccgggcttac acttccggct tgctggccgc gcttggatcg ggtcaattca
    14041 ggcggccgct cgtgctggcg gccttgttct gtttgcttgc cgcggcggcg gcgatggtgc
    14101 tgctgcaagc cgtgttcatc tcgaacggcc acagcccctg gggcttcgag gccgggcagg
    14161 cctggagcat cgaccgcgat ctgatgcgcg gcatccgata cgagaactcg ccttggtctc
    14221 caacgtatcc gcttccgtcg gagctgttct tccttccact ggccctcctc agccaggagg
    14281 ccgcacaggt gatctcaatc gtgttcactg cggtcctcat cgccggcgca ctcgtgctct
    14341 ggaatcagac ctctcacgca ccctggacat cgcttctgcc cctctttatt tcggtgccgg
    14401 tgctaggtgc tgtccagatc gaccatccgt acagcgcagt gggactggcc ggcctcgggc
    14461 tcgccgcgtg ggcatatcgg cggcagtcgt ggctcctctt aggtgtcgga gcggcgcttg
    14521 ctctgatccg gccgcaaaat gcgcttgccg cgctacctgt ggtcctcatt ggcctaagcg
    14581 gcagggcggc cattcgtacg gggatgggtg ccgccgccgt tctcgtccca ctggtagccg
    14641 cttcgttcct ctgggatccg ggctggttcg gggcctacca ggaggacgtc cgcctctacc
    14701 cgtcgtccgg aatatatggg ctcctcgtgc gctcgatcgg cgtcgtgggc ataagcgcca
    14761 tcctcctggc actcggcgtg ggcgggctgt ggcttcgccg gctcgggcac gatcccctcg
    14821 acgccttcac gctggtcctc gcggcgacag ttctcgtggt ccccgacccg gggctttact
    14881 gtggcttgtt cgttctgccg gctctgcagc gcataggggc ccggcccgaa tatcgggccg
    14941 tgccatgggt tgcaagcgcg agcgcttggg cgataccgct tgcggtcagc ccttggctgc
    15001 ttgcgaatcc gcccgccatt ttcccgctgc tcagcgcact cgcctactgg ctggccattc
    15061 agacctatcc cctgctgcgc cgcggacaca agcctcaaga cggcggaccc tgatatttct
    15121 gctcgctttg tcggcgtcat ccggccacgc ttaattgatg tcggcctggt tgaaaaacat
    15181 ggcctccgca agtagccggc gagtgccggc taagagtttc atctacaagg cactccatcg
    15241 ttcgccactc cctcccgaat gttcaaaggg tcctgcgcag gaaggaaata gtcctctagc
    15301 cgcgccgcta tgcttcggag actgacgctg aacgcataat aggtgcagca acaaatggtt
    15361 atccgctcga ccgcggcgct cgcttttgtc tgtcggcggg gacgcctggt tactgccagt
    15421 ttcagtttca gagattccca ggaatcgcgc gcgacgtgag gaggtctcgg tgaccatgcc
    15481 aacccaccaa gtgcgacttg tactgacggt aagcgactgc gaccacgcga cgacgttctt
    15541 tcgggatgct ctcggtctga aggaacgtac gactcttagg acccgatggg attcaactga
    15601 cgctattcat cccctccgag gaatcgctac ctggctacgc cgccaggccg ctcagtctcc
    15661 ttgactgggc tcgagtcccg ccagaagttt cttgaggaga ccggcaaggg catcctgctc
    15721 ctccttggac aggctcgcca gcatctcgtt ctcacgaccg gtgttggcgt cgacggcctg
    15781 gtctaggacc tcgcggccgg catcggtgag ctcgaccaac acgccccgac ggtcatcggg
    15841 gctgcggctg cgcttcacat agccgcgccg ctccagccgg tcgaggcggc tggtgatgcc
    15901 cgctgacgag agcatcaggc ccttgaacag ctgagtaggc gacaactgct ccttggcccc
    15961 ggcgaaacgc agcgcggcga ggacgccgac ctcgccccgg ttgagaccga accggctgaa
    16021 ggcaccctcc tgctgctggg cgatgtgcag gccgatccga gagatgcgag ccgtcacctg
    16081 ataggccccc atgtcctggc ggggacggtc agcggtccag cggtccagga gggtgtcggt
    16141 ccagtccttt gccaccgacc aactatacgg aatagctctc tcgcaataaa gattctcttg
    16201 aacatgtatc tcgctggcga gatttatcgc gggtaagaag cgaccacccg ctcatttagg
    16261 aggctttcgc atgtattcga aacacgtctt cagagttggt ctcgtggccg ccgcgctact
    16321 cgccgcggcg tgcggcagct cggcgtccgg cgccgccgac ccatacggag cgccggccag
    16381 taccccgagc caggcgcccg cgcctgcaag cgcctacccg gcgccgactg ctgtgcctgg
    16441 caccaccatc gacgttggga gtacgcggct cggacaggtc ctggtcgacg gcgcaggact
    16501 caccctctac ctgttcctgg ccgattcggg cacgaactcg acctgcaaca gcagtgcctg
    16561 tgtgcagtac tggccgcccg tcctgacgac aggcgctccg caggcgggaa cgggcgtgag
    16621 tgcatccctt ctcggaacta cggcgcggca ggatgggacg accgaagtca cctatgccgg
    16681 gcacccgctg tactacttca tctcggacaa gaaggcgggc gacgtgagcg gccagggcat
    16741 caacggtttc ggggcaccct ggtatgtggt tgcgccgtcg ggaatgcgga tcggctgagc
    16801 gccggccgga attgggacgc caccggttgg gcaaatggtc ggctcatgcc ggtggcttcc
    16861 cccattccag actgtgaaat ccgggcggct ggcatgggga caggggttca cctgaccaga
    16921 ggcccaaggc ctccatcgtg ctgactccct ctttttggtc cccgaactcc tcaaccagca
    16981 ggtcggtggc atcgcagcgc actttcccgt acacgattgt gcaggccgta gaattcggca
    17041 cggcgaccgg cggcgcactc gctggtcagt cgcgcagccg gggacgccag cgggtatagg
    17101 gaggggtttg ccaggaatgc tgcagccgat tgcgggacga gtggccgtcg tcaccggtgg
    17161 cagcagaggc atcggcaagg gcatcgcccg aacgcttgcg cggcatggcg tccgcgtgtt
    17221 gatcgcgtct cgtggcgagg atgccgctcg cggggtggtt gacgagatcc ggagcgcggg
    17281 agggaatgcc gaatacgtca ctgccgacgt cagcgccccc gatgacgcgc cccgcatcgc
    17341 cgaggccgcc ctcgaacact tcgggggcat cgacatcctc tgctcgaacg ccggcatctt
    17401 tccgccaagg tcgctgcgcg agctaacagc cgccgcaatc gacgaggtct tcaacgtcaa
    17461 tctccgcggc accatgcttg tggtcaaagc gtgcctgccg gcactggagc ggagcggcca
    17521 cggcagggtg atcctgactt catcgatcac cggtcccgtc accggctatc cgggatggag
    17581 ccactatggt gcgagcaagg ccggtcagct cggcttcatg cgaacggttg cgatggaggt
    17641 cgcaagcgct ggaatcacgg tcaacgcggt gctcccgggc aacatcaaga ccgagggtct
    17701 ggccgacctg ggcgaggaat acgagcgaac catggccgcc gcgatcccgc tcaggaggct
    17761 gggcaccgtc gacgacgtcg ccaacacggt cgtcttcctc gccagcgacg aggccgccta
    17821 cctgacgggc gtcgcgatcg tggtcgacgg tggccagatc cttcccgagt cgctggcggc
    17881 acttgagggt tagccaatcc ccaaagtcgt cggtgtattc gtgccggtca tcaggatcgc
    17941 cgccgcccag ttctcggtca gcggcgacaa ggctgacaac ccagccgtag tcctcgatcc
    18001 gcgtcgtggc ctaaggagct acgtggagat cgccgccagg atcgtttagg gcgggatcgt
    18061 ccaggcacat aggacaaatg tccccaccag gtcgtgacag gcgacccctt ccgcttgacg
    18121 atcagtttgc ggatactcgg gtcggctatc ggcccagcta cgcctatcgg tgggggtaaa
    18181 ccgcaccttg gcgactgggg cacagccgta ttcgtcagat cgcgagggga gagaagtacc
    18241 agatggttga gtcgaaatgg cgccggctgc ggacgattcc ggtcgcgcta ctgcttgcat
    18301 tgtcctcggc cgggtcggcc ttcacgccgg tcatggccgc cacgagcgtc acccaggtcg
    18361 acttcacgca gtgcgcgaac gacccagtgc ccagtgccgc cacgaactgc ccgggaggtt
    18421 ggatcaacgg catcctgcag ggcagcaact cgcactacgc ggaggacctg gtggtcccgc
    18481 agcggatcgt ggtcgacgtg ccggggacgc tgacgggggc aaccacgcac accctgacgg
    18541 tcaggtacca ggcccgcaag gccagcaccc acgcgtacga ttccatcgcc acctggaact
    18601 acacccagac gggcgctgac cgctgccaga gccttgccac gggcacctgc ccgaccggct
    18661 cggcgagcac cttcgccatc ccctttgact gcaccgaggt gccggcggcc accctccccg
    18721 gcaccgccca gggcggctgt tcgtcatcgg cgaacgatgt gactgcccgc caccagctgg
    18781 ccggccaggt cgccaccatg tacggcggcg ccctgacgga tatcaccgcg ccggtccacg
    18841 actcggctgc ggcgccgggg accgatgact acgcgaccgt cacgttttcc tataccgtcg
    18901 cagcgagcac ggcggccgac aactacgtcg agctgctctt cggcggtcac ctcgccgccg
    18961 gcttgcgcag ccgtggctgg ggcgtcggcc tcggtgcctc gtccatcaac ggcggcccct
    19021 accacttcaa gtgggacctg tccgacggcg cctcgatcgg caaccgggac aaccagatct
    19081 ccgccggcgg catcatggcc gctccgacgg tcagcaccct cctctccgcg agcagcgtcg
    19141 gcgcgggcac gcccgtgacc gactcggcaa ccctgatcgg cgccgcctcc aacgcgggcg
    19201 ggaccgtcac ctacaacgtg ttcgcgggga acgtctgcac gggcaccccc gtcttcacca
    19261 gcacggtgac cgtgaccggt gcggtggtgc cgccttcggc gccgttcaac accgccctgg
    19321 cgccggccgc gccgggcagc taccagtggc aggcctccta cag
//
LOCUS       DATCQR010000158         3684 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_39837, whole genome shotgun sequence.
ACCESSION   DATCQR010000158 DATCQR010000000
VERSION     DATCQR010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3684
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39837"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..658
                     /locus_tag="VIN32_05285"
     CDS             <1..658
                     /locus_tag="VIN32_05285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8922026.1"
                     /translation="LESVLEKGLGTAPPVLLNAFATPTTVPPSELGAYTGGAPPTEGA
                     VSPVTQASVYAAAIGAASCLPNVSGLLLDRLVDDGAAPAPATGLYYASGHAKPSAAAV
                     KGAIRTLARGAVVCPGLATRVTPTTLTFPEALSRSTAAVVVLGCSRDCLYLVTLDSAG
                     GQPVVARRGALNGGDPARTITLPKRPLRPGGYRLDVRLVSRVNPGAVTRQRSALLTVG
                     "
     gene            703..775
                     /locus_tag="VIN32_05290"
     tRNA            703..775
                     /locus_tag="VIN32_05290"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:736..738,aa:Thr,seq:cgt)
     gene            1218..2186
                     /locus_tag="VIN32_05295"
     CDS             1218..2186
                     /locus_tag="VIN32_05295"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEY8922027.1"
                     /translation="MTVVEASTSPPALPRWFGPRASPDDPAVSALIARVARGAGWADI
                     GGAFNLNVRIDAEPPVVLRVHRPWVRRGRVAGLRRLRERLQRTQVRVARPIRISGCDL
                     LRVDGRWAEVEEFIDHVQPRADEDSYVRLFEELGRLHTALKAVWEPSPPEPLDDHRTF
                     GQLRYSVGFTRRRLGPRSEPVVHRMRQLTGELSKLRKEVELPCTPIHGDYKLGNVGEL
                     SDGSWATFDLDFARVRERLYDIAASLALAQGGELLEPRRLLDAYEGTAPEPLTRDEHR
                     WLPGALALVPLHWAATAGLVGDGIHEAESAMTAAEAWWSRRAELSS"
     gene            2338..2640
                     /locus_tag="VIN32_05300"
     CDS             2338..2640
                     /locus_tag="VIN32_05300"
                     /inference="COORDINATES: protein motif:HMM:NF024751.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEY8922028.1"
                     /translation="MSAVSERFGPNLKTHKHARLKAFMAAHNIEPAYTPTYSSWLNAI
                     EAHFTSLKKFAIGGTDDPSHEYRRWRIARYLTWRNKEKGSSKAALAQFSRVRLYRH"
     gene            2798..>3684
                     /locus_tag="VIN32_05305"
     CDS             2798..>3684
                     /locus_tag="VIN32_05305"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEY8922029.1"
                     /translation="MTGIEASTSPPPLPRWFGPRASPDDPAVSALIARVARGAGWADI
                     GGGFNLNVRIDAEPPVVLRVHRPWVRRGRVAGLRRLRERLQRTQVRVARPIRISGCDL
                     LRVADRWAEIEEFIDHAQPRADEDSYVRLFEELGRVHTALKAVWEPSPPEPLDDHRTF
                     GQLRYSVGFTRRRLGPRSEPVVHRMRQLTGELSKLRKEVELPCAPIHGDYKLGNAGEL
                     SDGSWVTFDLDFARVRERLYDIAASLHQVAPSGELIEPRRLLDAYEGTAPEPLTRDEH
                     RWLPGALALVPLHWAATAGL"
BASE COUNT          598 a         1253 c         1229 g          604 t
ORIGIN      
        1 gctcgagtcg gtgctcgaga aagggctggg aacggcgccg ccggtcctgc tcaacgcgtt
       61 cgccacgccg acgacggtgc cgccgtcgga gctgggcgcg tacacgggtg gggcaccgcc
      121 gaccgaaggt gcggtctcgc cggtgacgca ggcttcggtc tatgccgcag cgatcggcgc
      181 cgcgtcctgc ctgccgaacg tcagcgggct gcttctcgac cggctcgtcg acgacggcgc
      241 agcgcccgcg ccggcaaccg ggctctacta cgcgagcggc cacgcgaagc cgtcggccgc
      301 cgccgtcaag ggggcgatcc ggaccctcgc gcgcggggcc gtggtctgcc ccgggctcgc
      361 gacgcgcgtc accccgacca cgctgacgtt cccggaggcc ctctcgaggt ccacggcggc
      421 cgtcgtcgtc ctcggctgca gccgcgactg cctctacctc gtcactctcg acagcgccgg
      481 cggccagccg gtcgtcgcgc gtcgcggcgc gctgaatgga ggcgatcccg cgcggacgat
      541 cacgctgccg aagcggccgc tccgtcctgg cggctaccgc ctcgacgtcc ggctcgtcag
      601 ccgcgtcaac ccgggcgcgg tgacgcgcca gaggagcgcc ttgctcacgg tcggctgacg
      661 acacagggga cgcggtgcgg cctggctaga ctggggctcc ccgccgacgt agctcagctg
      721 gtagagcaca tcactcgtaa tgatggggtc gggagttcga gtctccccgt cggcttcgag
      781 cgaatggcct gctaatgcgg gacttcttgg cccctaggcg gttcgcagcc cggacacccc
      841 ggtggccagt agcaacgaaa aagcaacgct ttggccccta gagactagga gggaatagcg
      901 aaacgtcgta gcctccgtgg cggagcggat ctgcggcggg ccgagatcac tcgcacccca
      961 ccatcgaagc tccttctacc ccatgagatg gctcagtacc cgcctcaggc gtaaggacgc
     1021 tcgcccaccc gagcgttcgg agagtgtcgg gcggcgtaga acggcagata tgtgcagcct
     1081 gatcgtcgag cgagaaggtg cgttgggcgc gttcgcgagc gtccctgaac ggcatatgcg
     1141 tggcacgatc gacccgcgtc gaggaggatg ggttcgctag gcggaccctt cgtcgggtgc
     1201 gctggtgggc cattgccatg accgtagtcg aagcttcgac ctctccccct gccttgccgc
     1261 gctggttcgg acctagagcc agcccagatg acccggccgt ctcggccctg atcgcccgcg
     1321 tcgcgagagg ggccggatgg gcagacattg gcggtgcgtt caatctcaac gtgcggatag
     1381 acgctgagcc gccggttgtc ctccgggttc atcggccctg ggtccgccgc ggccgggtcg
     1441 cgggactgcg gcgcctgcgg gagcggctcc agcggacgca agtccgcgtc gctcggccga
     1501 ttcggatttc cggctgcgac ctcctgaggg tcgatggtcg ttgggctgag gttgaggagt
     1561 tcatcgatca cgtccagcca cgggcagacg aggactccta cgtccgccta ttcgaggagc
     1621 tcggacgcct tcacactgcg ctcaaagcag tctgggagcc cagtccgccc gaacccctcg
     1681 acgaccacag aaccttcggg caattgcgtt actcggtcgg cttcacgcgc cgcaggctcg
     1741 gaccccgcag cgagccggtc gtgcatcgga tgcgccaact gactggcgag ctctccaagc
     1801 tccggaagga ggtcgaactg ccatgcactc cgattcatgg ggactacaaa ttgggcaacg
     1861 tgggagaact gtcagacggc tcatgggcca ccttcgacct ggattttgcg agggtcaggg
     1921 agcggctgta cgacatcgcg gccagcctgg ccctcgcaca gggcggcgaa ttgctcgagc
     1981 cgcgacgact gctcgacgcc tacgaaggca cggctccgga accgctgact cgcgacgagc
     2041 accgttggct ccccggggct ctagcccttg tacccttgca ctgggctgcc accgcagggc
     2101 tcgtgggcga cggcatccac gaggccgaga gcgcaatgac cgccgccgag gcctggtggt
     2161 cgcgccgggc tgaactctcg tcgtagtccg gcggccgcgc tcggggactg gagttctcgg
     2221 ccgagctccg actccactga agcggctggc atgtcgcgct aaccaacctc tgcgatgaat
     2281 gcgtggagga gcgtaccctc gggcgtgcct tagcctccca ccccggccgg cagtcgcata
     2341 agtgccgttt ccgagcgttt cggacccaac ctcaagacgc acaaacacgc tcggctcaag
     2401 gcgttcatgg ctgcgcacaa catcgagccc gcctacacgc cgacctattc gagctggctc
     2461 aacgccatcg aagcgcactt tacgagcctc aagaagttcg cgatcggggg cactgacgat
     2521 ccgagccacg agtaccggcg ctggcgtatc gcccgctacc tgacctggcg gaacaaggag
     2581 aagggcagct ccaaggctgc cctggctcaa ttctcacggg ttaggttgta tcggcactag
     2641 ctagtgccca gtagctcgac cgaccagcta ctcgtctcta cgatggtgat caggcgagct
     2701 gaaagggctg cggggtgccg gccgtcatag cgtcgcggca cgattgcgcc gtgctcgccg
     2761 gcggaccatt cctcgggtac gctggtgggc cattgccatg accggaatcg aagcttcgac
     2821 ctctccccct cccttgccgc gctggttcgg acctagagcc agcccagatg acccggccgt
     2881 ctcggccctg atcgcccgcg tcgcgcgagg ggccggatgg gcagacattg gcggtgggtt
     2941 caacctcaac gtgcggatag acgctgagcc gccggttgtc ctccgggttc ataggccctg
     3001 ggtccgccgc ggccgggtag cgggactgcg gcgcctgcgg gagcggctcc agcggacgca
     3061 agtccgcgtc gctcggccga ttcggatttc cggctgcgac ctcctgaggg tcgctgatcg
     3121 ttgggctgag attgaggagt tcatcgatca cgcccagcca cgggcggacg aggactccta
     3181 cgtccgccta ttcgaggagc tcggacgcgt tcacactgcg ctcaaagcag tctgggagcc
     3241 cagtccgccc gaacccctcg acgaccacag aaccttcggc caactgcgtt actcggtcgg
     3301 cttcacacgc cgcaggctcg gaccccgcag cgagccggtc gtgcatcgga tgcgccaact
     3361 gactggcgag ctctccaagc tccggaagga ggtcgaactg ccatgcgctc cgattcatgg
     3421 ggactacaaa ttgggcaacg cgggagaact gtcagacggc tcatgggtca ccttcgacct
     3481 ggatttcgcg agggtcaggg agcggctgta cgacatcgcg gccagcctgc accaggtcgc
     3541 accgagcggc gaattgatcg agccgcgacg actgctcgac gcctacgaag gcacggctcc
     3601 ggaaccgctg actcgcgacg agcaccgttg gctccccggg gctctagccc ttgtaccctt
     3661 gcactgggct gccaccgcag ggct
//
LOCUS       DATCQR010000159         8538 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_179357, whole genome shotgun sequence.
ACCESSION   DATCQR010000159 DATCQR010000000
VERSION     DATCQR010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 8538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8538
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179357"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(99..869)
                     /gene="paaC"
                     /locus_tag="VIN32_05310"
     CDS             complement(99..869)
                     /gene="paaC"
                     /locus_tag="VIN32_05310"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: protein motif:HMM:NF016993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaC"
                     /protein_id="HEY8922030.1"
                     /translation="MTDLDSETRDALAELLLAMADDEFVLGFWDSEWTGIAPMLEEDV
                     AMSSVSQDEIGHAKALYELLAELTGEDADRIAFGRPVDGYRHAALMNHARTDWAFSIA
                     RRFLYENADAVRLESLAASSYQPLADLVVKMRREETYHLLHFDLWLRRLAEGGEESRG
                     RLTEAIERLWGDAQAVFAPLAGEASLVAARILPESLETLRARWLERVGALLEAAHLPV
                     PAVSAHPDADGRTRRTDEFTWLHGEFTMVSGSEADASW"
     gene            complement(866..1186)
                     /locus_tag="VIN32_05315"
     CDS             complement(866..1186)
                     /locus_tag="VIN32_05315"
                     /inference="COORDINATES: protein motif:HMM:NF018002.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8922031.1"
                     /translation="MPAPHDRVWEVFRQEAEGDPMVHGGSVKAPDEELATHYAREFYG
                     RRQESERLWIVPRDAITELDDRDLLKPPFDRSFKRPGGFLIKHKLEAARERAGAPQAE
                     EVGE"
     gene            complement(1400..2320)
                     /gene="paaA"
                     /locus_tag="VIN32_05320"
     CDS             complement(1400..2320)
                     /gene="paaA"
                     /locus_tag="VIN32_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012810204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaA"
                     /protein_id="HEY8922032.1"
                     /translation="MDDVARADAFYAHVSGGGKVEPDDWMPDDYRAAVLKFVEMHANS
                     ELMGVLPEREWILRAPTLRRKLALTSKVQDEVGHAQLLYRVAEDLGKSREQMLQDLIA
                     GKTKFHNVFHYPTRSWADVGIIAWLVDAAAIVSQQALRDSSYAPYARTMKKICWEESV
                     HIMHGRDVVLTLMNGTDEQRAMVQDALDRWWGPLMQMHGPPTNPATDRDLIWKIKAKG
                     NEQLRQEFLSIYVPRIRELGLVIPDPALAYDEEAGRWAYTEPDWDELRSVVTGHGPKS
                     QERLEFRRDARATTQWVRDAIIGPVGVAAA"
     gene            2421..2828
                     /locus_tag="VIN32_05325"
     CDS             2421..2828
                     /locus_tag="VIN32_05325"
                     /inference="COORDINATES: protein motif:HMM:TIGR03618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03618 family F420-dependent PPOX class
                     oxidoreductase"
                     /protein_id="HEY8922033.1"
                     /translation="MPTAIDEVPAELVDLLTGSTLGHVTVITPKGALATHIMWVDYED
                     GRVLTSSRVGSAKGKALRADPRIAVSVMDRANPWRWVSISGRVVEIRPDTELAFIDKL
                     SRRYTGRDYMMRTMEREVFVIEPDRIRSATGRG"
     gene            complement(2832..3206)
                     /locus_tag="VIN32_05330"
     CDS             complement(2832..3206)
                     /locus_tag="VIN32_05330"
                     /inference="COORDINATES: protein motif:HMM:NF021525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HEY8922034.1"
                     /translation="MSLPFDARGLAMLREEDEIEIETAVGDEGPTHRAIIWVVVDEQD
                     RVLIRSYRGPGARWYREITAKPEARVHVRGRALAVRAIPAADPDRVAACSEGLRRKYA
                     GHGSMPQMLRKHLETTLELLSR"
     gene            complement(3203..3463)
                     /locus_tag="VIN32_05335"
     CDS             complement(3203..3463)
                     /locus_tag="VIN32_05335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8922035.1"
                     /translation="MTEQEPAVEVKPGDRLVSARGRRIGRVEAVFADYLLVRTGFILP
                     VDLYVPREAVSSSGGALRVELERKAAYDAWHRPLKRAPHDEP"
     gene            complement(3460..4413)
                     /locus_tag="VIN32_05340"
     CDS             complement(3460..4413)
                     /locus_tag="VIN32_05340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEY8922036.1"
                     /translation="MPAPTAPLFIVNPVAGSGRAHRIVPRIEAWLAERGIEARLLETR
                     EAGHAERLAAAAADLGHDRVIAVGGDGTIQEVINGLLASGIGTDGGTPALGLIPSGRG
                     NDLARSVHLPIDPMACLPIAVGETTHPFDVGSARGEGGGQRYFGAAGGAGFDAAVAYT
                     MAVRRRFWMRGEAGYFLGTLNELRRYRNSELRVTLIGNGGVRVVSQRFLFIAFANGSY
                     YGGGMQICPDAETDDGLLDVCLVGDLSRLEALRELPGIYQAKHLRNPMVEIVRARSVR
                     IEGDGATRVHLDGEPFGSVPVEISLLPGAISVAVAAAVAVQ"
     gene            complement(4451..6001)
                     /locus_tag="VIN32_05345"
     CDS             complement(4451..6001)
                     /locus_tag="VIN32_05345"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEY8922037.1"
                     /translation="MATTRKPPVPSRAAAAVAGIVTGGVALGATELLAGILPGAPSVV
                     LEIGAFLISLQPAGAKQVVVDLFGTADKLALNLAIVVGALVLAAGLGILARYHRPWTY
                     AGFAAIAVLGLLASLRDSLVDPLLALVAAAVGIVVAYYLLGWLLRLATPAGETTAEMP
                     DWGRRRFIGTAVGVAAGAVVGGTIGRTLLNSRVDAAPTAASIPRAIDPVPPLPAGSEL
                     AAAGISPLVVKNQNFYRIDTNLLTPRIDVAGWMLSVDGMVDHPFTITYAELLAMEMHE
                     QYVTIACVSNEVGGDLVGNALWKGVRLRELLDRAGVQAGATQIVGHAFDGWTAGFPTA
                     WLDDPAREALVAVAMNGDPLPAEHGFPARLIVPGLYGYVSATKWLAKIGLTTLEAFNG
                     YWVPLGWAKEAPILTQSRIDVPKGGGSLNPGEVAVAGVAWAPNRGIAKVEVQVDGKDW
                     APAELSVPISDATWVQWLFRWTATPGTHTLSVRATDGTGEVQTADVTRPAPDGARGHH
                     TIDVSVGA"
     gene            6053..6565
                     /locus_tag="VIN32_05350"
     CDS             6053..6565
                     /locus_tag="VIN32_05350"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY8922038.1"
                     /translation="MAHLYLSHGASGSAAGLAPQIVGLRRRGVEATAVQLPKGSAERA
                     MPVYLDAAAPSADTAIGGHSFGGRVASLLAAEHEYGALVLLSYPLHAPGGAERWEERT
                     AHWPHIGCPVLLLSGDRDPFARIGLLRDAVRRLPSAKLIVYPGLGHGIGPVFEEALDE
                     VAAFLKSTLA"
     gene            6586..>8538
                     /locus_tag="VIN32_05355"
     CDS             6586..>8538
                     /locus_tag="VIN32_05355"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY8922039.1"
                     /translation="MVTCSTCGAESSPRFRVCGQCGAQLAENRPPEEIRRIATVVTSD
                     LKGSTALGEKLDPESLREVLTRYFDEMRLVFESHGGTIEKIIGDAIVAVFGLPTRGDD
                     DALRAVQAAAESMRALAVLNVQLEEAWGVRLVTRTGVASGEVTVGELTAGAHVLTGET
                     MGLAGVMEQNAPPLEVLLADSTYQLVADHLEAEPYGPVFAKGSQEPLAAHRLLTVSGP
                     TAGVEIPEGETDAGTQICGVCGEQNPIEHRFCGTCGTAMTGRVKFQESRKTVTVVFAD
                     PKPSMKDGASPSPEALRDVMSRYFEGMQRVLDRHGATVEKFIGDAVMAVFGLPIRHED
                     DALRAVRAAADMQLALLELNAAFELEWGVSLGNHIGVNTGEVVAGDASLGQRLVSGDT
                     VNVAARLEQAAGPGEVLLGGLTYRLVRGAVLVEPVAPLTLKGKAEPVSAYRLTAVRER
                     AEGFERRSDSPMIGRDAEMAELRAVIAEAIEVHGCRMVTVVGDAGLGKSRLIAEFTTE
                     QIERATVIRGRCLPYGEGITFWPLREAVREAAAIEPNDAPDVALRKLDQLVPDAEVAG
                     RLASAIGLSIEPYPVAEVVWAARRFLEGLAEVRPVILVVDDIQWASPTFLELMGNLIE
                     MVTDASVLLLCTSRPNLLEAHPDWATD"
BASE COUNT         1325 a         2962 c         2891 g         1360 t
ORIGIN      
        1 gatcacgccc aggtcgacca ggctgatggc agggatctcc gggtccgcga tctcgcccag
       61 tgccgaccac acagccgact cgcgggttgg cacggtggct accacgaggc gtccgcctcg
      121 gagccggaca ccatcgtgaa ctcgccgtgg agccaggtga actcatcggt tcgccgcgtc
      181 cggccgtccg cgtcgggatg cgcgctgacc gcgggcacgg gcaggtgcgc cgcctccaac
      241 agcgcgccaa ctcgctccag ccagcgcgcg cggagcgtct ccagcgactc cggcaggatc
      301 cgggcggcga ccaggctcgc ctcgcccgca agtggcgcaa agactgcctg cgcgtcgccc
      361 cataaccgct cgatcgcctc cgtgagcctc ccgcgtgact cttcgccacc ctccgccagg
      421 cggcgcagcc agagatcgaa atggaggaga tggtaagtct cttcgcgtcg catcttgacc
      481 accagatcgg ccagcggctg gtacgaggag gcggccagcg attcgagtcg caccgcatcg
      541 gcgttctcgt acaggaagcg gcgggcgatg ctgaaggccc aatcggtgcg agcgtggttc
      601 atgagcgccg cgtgccggta cccatccacc gggcgcccga aggcgatccg gtccgcatct
      661 tcgcccgtca gctcggccag gagctcgtag agggccttgg catgcccgat ctcgtcctgg
      721 ctgaccgagc tcatcgccac gtcctcctcg agcatcggcg caattccggt ccactcggag
      781 tcccagaagc cgagcacgaa ctcatcgtcg gccatcgcca gcagcagctc ggccagggcg
      841 tcgcgcgtct cgctgtccag gtccgtcact cccccacctc ctcggcctgc ggagcgccgg
      901 cgcgctcgcg cgctgcctcc agcttgtgct tgatcaggaa tccgccggga cgcttgaacg
      961 accgatcgaa cggtggcttg aggaggtcgc ggtcatccag ctctgtgatc gcatcgcgcg
     1021 gcacgatcca gagccgctcg ctctcctgcc ggcggccgta gaactcgcgg gcgtaatggg
     1081 ttgccagctc ctcgtccggt gccttcacgc tgccgccgtg gaccatcggg tcaccctcgg
     1141 cctcctggcg gaagacctcc cagacccggt cgtgcggtgc aggcattctc gaagcgtacc
     1201 gcgcgagtgg ctgttgcgca ctgttgcagg tgagacatct cgccagatcg caaccaggtt
     1261 cacgttaggc ggtcaaacgg tgtgcggcgg cgacagaggc gacccttcgc acgctggagt
     1321 tacgaagcag cggagcaacg gaccgcccgg gaagatgcct taggacaacc tggttgcaat
     1381 atggcgataa cgtgagcagt caggcggcgg cgaccccgac cgggccgatg atcgcgtcgc
     1441 ggacccactg ggtggtggcg cgcgcatcgc gtcggaactc cagccgctcc tgcgacttcg
     1501 gcccatggcc ggtgacgacg gaccgcagct cgtcccagtc gggctcggtg tacgcccagc
     1561 ggccggcctc ctcgtcgtag gcgagggcgg gatcggggat caccaggccg agctcgcgga
     1621 tgcgcggcac gtagatcgac aggaactcct ggcgcagctg ctcgttgccc ttggccttta
     1681 tcttccagat caggtcgcgg tccgtcgccg ggttggtggg cgggccgtgc atctgcatca
     1741 gcgggcccca ccatcggtcg agggcgtcct gcaccatggc acgctgctca tcggtcccgt
     1801 tcatgagggt gagcaccaca tcgcgtccgt gcatgatatg gaccgactcc tcccagcaga
     1861 tcttcttcat cgtccgcgcg tacggggcgt aggaggagtc gcggagcgcc tgctggctga
     1921 cgatcgccgc cgcgtccacc agccaggcga tgatcccgac gtcggcccag ctgcgggtcg
     1981 ggtaatggaa gacgttgtgg aacttggtct tgccggcgat caggtcctgc agcatctgct
     2041 cgcggctctt gccgagatcc tcggcgactc ggtacaggag ctgggcatgc cccacctcat
     2101 cctgcacctt gctggtgagc gccagcttgc ggcgtagagt cggagcgcga aggatccact
     2161 cgcgctcggg gagcacgccc atcagctcgc tgttggcgtg catctccacg aacttgagca
     2221 ctgcggcacg atagtcatcc ggcatccagt cgtctggctc gaccttgccg ccgcccgaga
     2281 catgggcgta gaacgcatcg gctcgtgcta cgtcgtccat gccgcctagg gtagcgcaag
     2341 accgattgag tggcagcccg ccgcacgggg cggtacagtt gccccgtcaa ctacttcgaa
     2401 gaagtcggga gcttcgccca atgcccaccg ccatcgatga ggtgccagcc gagctggtcg
     2461 acctgctcac cgggagcact ctcgggcacg tcaccgtcat cactcccaag ggagccctcg
     2521 ccacccacat catgtgggtc gactacgagg atggccgcgt cctgaccagc tcccgcgtcg
     2581 ggtccgccaa gggcaaggcc ctgcgcgccg atccccgtat cgcggtctcg gtgatggacc
     2641 gcgccaaccc gtggcgctgg gtctcgatca gcgggcgggt cgttgagatt cgcccggaca
     2701 ccgagctcgc cttcatcgac aagctctctc gccgctacac gggtcgcgac tacatgatgc
     2761 ggaccatgga gcgggaggtc ttcgtcatcg agccggatcg aattcgctcg gcgactgggc
     2821 gcggctgacc ctcagcgcga caggagctcc agggtcgttt cgaggtgctt ccgcagcatc
     2881 tggggcatgg acccgtggcc cgcgtacttg cggcgcaggc cctcggagca ggcggcgacc
     2941 cggtcggggt cggcggcggg gatggctcgc accgccagcg ctcgaccacg aacatggacg
     3001 cgggcctccg gcttcgccgt gatctcgcgg taccagcgtg cgccggggcc tcggtagctg
     3061 cggatcagca cccggtcctg ctcatcgacg accacccaga tgatggcccg atgggttggg
     3121 ccctcgtcgc cgacggccgt ctcgatctcg atctcgtcct cttcacgcag catggcgagc
     3181 ccgcgcgcgt cgaagggcaa gctcatggtt cgtcatgggg agcgcgcttc agcggccgat
     3241 gccacgcgtc gtacgccgcc ttcctctcca gctcgacccg cagcgcgcca ccagagctgc
     3301 tcaccgcttc ccgaggcacg tagaggtcta cgggcaggat gaagcccgtc cgcaccagca
     3361 ggtagtccgc aaagacagct tcgaccctgc cgatgcgacg gccgcgcgca ctgacgagcc
     3421 gatcgcccgg cttgacctca acggccggct cctgctccgt cattgcaccg cgactgcggc
     3481 tgcgaccgcg acgctgatgg ctccggggag gaggctgatc tccacgggca cgctgccgaa
     3541 gggctccccg tcgaggtgaa cgcgggtcgc accgtcgccc tcgatccgaa ctgagcgggc
     3601 acgtacgatc tcgaccattg gattcctgag atgcttcgcc tggtagatgc ccggcagctc
     3661 ccgcagagcc tcgagcctgg acaggtcgcc gacgaggcag acgtcgagca ggccgtcgtc
     3721 cgtctcggca tccgggcata tctgcatccc tcctccgtag tacgaaccgt tggcgaaggc
     3781 gatgaacagg aagcgctggc tgaccacgcg cacccctccg ttgccgatga gcgtcacgcg
     3841 cagctcggag ttgcggtatc ggcgcagctc gttaagcgtg ccgaggaagt agcccgcctc
     3901 gccgcgcatc cagaagcggc gtctgacggc catcgtgtac gccacggcgg catcgaagcc
     3961 ggcgcccccg gcggccccga agtagcgctg gcctccgccc tcgcctcgag cgctgcccac
     4021 gtcgaagggg tgcgtggtct cgccgacggc aatggggagg caggccatcg gatcgatcgg
     4081 cagatggacg ctgcgcgcca ggtcgttgcc gcgaccggag gggatgaggc ccagcgccgg
     4141 cgtgccgcca tcggtcccga tcccagaggc cagcaggccg ttgatcacct cttggatggt
     4201 gccgtcgccg ccgaccgcga tgacccggtc gtggccgagg tccgcggcag ccgcagcgag
     4261 acgttctgcg tgccccgctt cccgagtctc gagcagtcgc gcctcgatcc ctcgctctgc
     4321 caaccaggcc tcgattcgcg ggacgatgcg gtgcgcgcga ccgctgccgg cgacagggtt
     4381 gacgatgaag agaggcgccg tgggggctgg cacgcccccg attctgcgct gggcgtcaag
     4441 gcgtttgagg ctaggcgcca accgagacgt cgatggtgtg atggccgcgt gcgccatccg
     4501 gcgcggggcg cgtcacgtcc gcggtctgca cctctcccgt cccatccgtg gcgcgcacgc
     4561 tgagcgtgtg tgtgccgggc gtggcggtcc aacggaagag ccactgcacc caggttgcgt
     4621 cggagatcgg gaccgacagc tctgccggcg cccagtcctt gccgtcgacc tgcacttcga
     4681 ccttggcgat gcccctgttc ggagcccagg cgaccccggc gacggcgacc tcccctggat
     4741 tgagggaccc tccacccttc ggaacgtcga tgcgcgactg ggtcaggatc ggcgcctcct
     4801 tggcccagcc gaggggcacc cagtagccgt tgaaggcctc gagtgtggtg aggccgatct
     4861 tggccagcca cttggtggcc gacacgtagc cgtacagtcc gggcacgatc aggcgggctg
     4921 gaaagccatg ctcggccggc agcggatctc cgttcatggc caccgcgacg agggcctcgc
     4981 gggcgggatc gtcgagccag gcagtcggga agccggctgt ccagccatcg aaggcatgcc
     5041 ccacgatctg cgtggcgccg gcctgcacgc cagctcgatc gaggagctcg cgcagtcgca
     5101 cgcccttcca gagggcgttg cccacgaggt ccccgccgac ctcgttgctg acgcaggcga
     5161 tggtgacgta ctgctcgtgc atctccatgg ccagaagctc ggcgtaggtg atggtgaacg
     5221 ggtggtcgac catgccgtcg acgctcagca tccagccggc aacatcgatc cggggcgtca
     5281 gcaggttcgt gtcaatgcga taaaagttct ggttcttcac cactagtggg ctgatccctg
     5341 ccgccgccaa ctccgatccc gcaggaagcg gcgggaccgg atcgatcgct cgcgggatgg
     5401 acgccgccgt cggagcggcg tcgacgcggc tgttgaggag cgtccgcccg atcgtcccgc
     5461 cgaccaccgc cccggccgcc acgccgaccg ccgtgccgat gaagcgccgg cgtccccagt
     5521 ccggcatctc tgccgtggtt tcgccggcgg gcgtggcaag gcggagcagc cacccgagga
     5581 ggtagtacgc gaccacgatg cccactgctg ctgccaccag tgcgagcagc gggtccacca
     5641 gggagtcgcg cagcgacgcc aggaggccga gcacggcgat cgccgcgaac ccggcatagg
     5701 tccatggacg gtgatagcgc gccaggatcc ccagcccggc agcgagcacc agcgcgccaa
     5761 ccacgatggc gaggttgagc gcgagcttgt cggccgtccc gaacaggtcc accacgacct
     5821 gcttggctcc agcaggctgc agcgagatca ggaaggcgcc gatctcgagc accaccgacg
     5881 gcgcgccggg caggatcccg gccagcagct cggtcgcacc gagcgccacc ccgccggtca
     5941 ctatcccggc gaccgcggcg gcggctcgcg aggggacggg aggcttgcgg gttgtggcca
     6001 tgcgagcgtc atgtgtacca cgctccgggc tccgccgctc taggcttgac ccgtggccca
     6061 cctctacctt tcccacggtg cgtccgggag tgccgccggc cttgcgccac agatcgtcgg
     6121 gctgcggcga cgcggggtcg aagcgaccgc cgtccagctg ccgaagggct ctgcggagcg
     6181 cgccatgccc gtctatctcg acgccgctgc gccatccgcc gacaccgcca tcggtggcca
     6241 ctctttcggg ggccgcgtcg cctccctgct cgccgccgag catgagtacg gcgccctggt
     6301 gctcctctcg tacccgctcc atgcgcccgg cggcgccgaa cgatgggagg agcgcacggc
     6361 gcattggccg cacatcggct gcccggtgct gctcctctcc ggcgaccgcg acccgttcgc
     6421 caggatcggg ttgctgcgcg acgcggtgcg gcgcctgccc tccgccaagt tgatcgtgta
     6481 ccccgggctc ggccacggca tcggtccagt cttcgaagag gcgctggacg aggtagccgc
     6541 cttcctgaag tcgacgctcg catgaaggcc cctacactca atgccatggt cacgtgctcg
     6601 acttgcggcg cggagtcgtc accccgcttc cgggtgtgcg gtcagtgtgg ggcgcagctc
     6661 gcggagaacc gcccacccga ggagatccgg cgcattgcga ccgtcgtcac ctcggacctg
     6721 aagggctcca cggcccttgg ggagaagctc gatcccgagt cgctccgcga ggtcctcacg
     6781 cgctacttcg acgagatgcg cctcgtcttc gaatcgcatg gtgggacgat cgagaagatc
     6841 atcggcgacg ccatcgtggc cgtcttcggg ctccccacac gcggcgacga cgacgcgttg
     6901 cgcgccgtcc aggcagctgc cgagagcatg cgcgcactgg ccgtgctcaa cgtccagctc
     6961 gaggaggcct ggggggttcg gctcgtcact cgcacgggtg tcgccagcgg cgaggtgacc
     7021 gtcggcgagc tgaccgccgg ggcacatgtc ctcacgggcg agacgatggg cctggcgggc
     7081 gtgatggaac agaacgcgcc tccactggag gtcctgctcg cggactcgac ctaccagctg
     7141 gttgccgatc acctcgaggc ggagccgtac ggaccggtct tcgccaaggg ctcccaggag
     7201 ccactcgccg ctcatcgcct gctcacggta tccggcccaa cggcgggcgt cgaaattccc
     7261 gagggagaaa ccgacgcagg aacgcagatc tgcggcgtat gtggcgagca gaacccgatc
     7321 gagcatcgtt tctgcggcac ctgcggcacg gcgatgaccg ggcgggtgaa gttccaggag
     7381 agccgcaaga ccgtaaccgt cgtcttcgcg gatccgaagc cctcgatgaa ggacggcgcc
     7441 tctccgagcc ctgaggcgtt gcgtgacgtc atgtcgcgct atttcgaggg catgcagcgt
     7501 gtgctggatc gccacggagc cacggtcgag aagttcatcg gcgacgcggt catggccgtc
     7561 ttcggcctgc ccatccggca cgaggacgac gcgctccgcg cagtccgtgc cgccgccgat
     7621 atgcagctgg cgctcctcga gctgaacgcc gcattcgaat tggagtgggg cgtcagcctc
     7681 ggcaatcaca tcggcgtcaa caccggtgaa gtcgtggcag gcgatgcgag ccttgggcag
     7741 cgtctggtga gcggtgacac cgtcaacgtc gcggcgcgcc tggaacaggc cgccggtccg
     7801 ggcgaggtgc tgctgggtgg tctcacctat cgcctggtcc gcggcgctgt gctggtcgag
     7861 ccggtggccc cgctgaccct gaaggggaag gcggagcccg tctcagccta tcgactgacc
     7921 gctgtccggg agcgtgcgga aggtttcgag cgacgcagcg actcccccat gattggccgc
     7981 gacgccgaga tggcggagct gcgcgcggtc atcgcggagg cgatcgaggt ccacggctgc
     8041 cgcatggtga ccgtcgtcgg agacgcggga cttggcaagt cgcgactgat cgccgaattc
     8101 accacagaac agatcgagcg cgcgaccgtg atccgtggcc gatgtctgcc gtacggggaa
     8161 gggatcacgt tctggccgct gcgagaggct gtccgcgagg ccgcggccat cgaaccgaat
     8221 gacgcgcctg acgtcgcgct ccgcaagctc gaccaactgg taccggacgc ggaggtggcg
     8281 ggtcgcctcg cctcggcaat cggcctgtcg atcgagccct atcccgttgc agaggtcgtc
     8341 tgggctgcgc gcaggttcct tgaaggactc gcggaggtac gcccggtgat ccttgtggtc
     8401 gacgacatcc agtgggcgtc gcccaccttc ctcgagttga tgggcaacct catcgaaatg
     8461 gtcaccgacg cctcggtcct gctgctgtgc acaagtcggc cgaacctcct ggaggcgcat
     8521 cctgattggg cgactgac
//
LOCUS       DATCQR010000160        14347 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_86697, whole genome shotgun sequence.
ACCESSION   DATCQR010000160 DATCQR010000000
VERSION     DATCQR010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 14347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14347
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86697"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(171..548)
                     /gene="rbfA"
                     /locus_tag="VIN32_05360"
     CDS             complement(171..548)
                     /gene="rbfA"
                     /locus_tag="VIN32_05360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEY8922040.1"
                     /translation="MSQRTDRLDSQIRQELMDLLQREMKDPRLGFATITRVETARDLG
                     HARVWVSVLGSDVERERTMDALRVATPWLRRKLGERLSLRHVPELTVREDDSTASGDR
                     VLRILQELDEGSTNPVPDEAGEP"
     gene            complement(559..2469)
                     /gene="infB"
                     /locus_tag="VIN32_05365"
     CDS             complement(559..2469)
                     /gene="infB"
                     /locus_tag="VIN32_05365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEY8922041.1"
                     /translation="MPPRRYQPRRGPRPPRRRDGGPEGGLAVETQPVVPRGGPPVELP
                     SSVSVTDLAAALTVSHADVIKELIRNGIFATINQSIDFDTASLVAGELGIETVERGVA
                     QRAAAEAASQQEIVAPGSEEEGAGKRVLFTEEEAAELVTRAPIVTIMGHVDHGKTSLL
                     DAIRSTSVATAERGGITQHIGASEVEHNGKRIVFLDTPGHEAFTAMRARGAQVTDIAI
                     IVVAADDGVMPQTKEAIDHVRAARVPMIVAINKIDKPDANPDRVKQELADNKVLIEEF
                     GGDVIAVPVSAKQKTGLDDLMEMILLVADLQDLKANPNRPAIGTIIESKVATGRGNVA
                     TVLVQTGTLKIGDVVTVGRTFGRVRALQNAAGKRVKTAEPATAVEIIGITDLPEAGDI
                     LRVVPDEKTARDMAEAVEQAATSGDGKAAFTLEDISAQIQASEVKELRVVMKTDVQGS
                     LGAIRHALERLNTAEVRINIVSEGAGDINESDVQLASASEAVVIGFNTKLDPGAQRAV
                     DALGVDVRLYDVIYKLTDDLGAALSGMLTPKQVEQIEGHAEVRMVIKAGKAGNVAGSY
                     VVDGRIVRGGQVRLFRGGQVVAEGRVESLKRFKDDVREVATGFECGIGLGNIEIAEGD
                     LIECYQMVAEQA"
     gene            complement(2456..2728)
                     /locus_tag="VIN32_05370"
     CDS             complement(2456..2728)
                     /locus_tag="VIN32_05370"
                     /inference="COORDINATES: protein motif:HMM:NF016204.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEY8922042.1"
                     /translation="MPKRRPEHQPQRTCAVCREVHPKRSMTRVVRRADGAVTVDLGGK
                     APGRGTYLCDQPACRDPHRLAEGIQRALGGTVVPEQILAEVTHASA"
     gene            complement(2709..3842)
                     /gene="nusA"
                     /locus_tag="VIN32_05375"
     CDS             complement(2709..3842)
                     /gene="nusA"
                     /locus_tag="VIN32_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HEY8922043.1"
                     /translation="MNRDFIGALLQLNAEKGVPQEILIETLEQAIESAYRRNADAPEN
                     VVVRVDPESGEISVFREYEVVATPDEVEDPELQMLVSAAQKIDPTTGVGAKVRVPENV
                     TQAQLGRIAAQTAGQVYRQRLREAERDVVYSQYASREGEIMTGVVHRTTDQAVVLDLG
                     KGVEAVLAVSEQLPGEHYRIGQHLKVYVLEVRRTTRGPVLVVSRTHRGFLRRVMEMEI
                     PEIYNGTVVIRGIAREAGSRSKVAVESRQVGVDAKGAAVGQRGARIQAIVAELNGEKV
                     DVVLWNEDPAQFVAEALSPAEVLNVRIDEEHKIANVVVPERQLSLAIGKEGQNARLAA
                     KLTGWRIDIRSDAGVATAAAAHGSAPDDAATPEEAPVDAEAEA"
     gene            complement(4013..5134)
                     /locus_tag="VIN32_05380"
     CDS             complement(4013..5134)
                     /locus_tag="VIN32_05380"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HEY8922044.1"
                     /translation="MFGTILAFIAVLFVLVLVHELGHFIVAKRAGITVQEFGVGFPPR
                     IGSIVWHGTRYSLNWIPLGGFVKMLGEDGDGEAEKMRKRGLSEASIDKAMEGAFNRKP
                     IWLRIVVLVAGVVMNFLLAATLFAVALSLPGTEGRGPLTVTEIQAGSPAIAAGLEVGD
                     VIVAADGRTFPVSRDLTAYVRSRAGHLVELAVRRDGAETLIPVTPRSLSAAEEAKGLG
                     AVGFSYEPDRFVEVPPTVASPAAAAWKGVVSAADLAAKIPGGLASAVAGLLGLAPDAG
                     TAVGPIGIASETGRVLQAPLVSQLVFVGILSVNLAVLNILPFPPLDGGRIAVTVVEAV
                     RRRRLPAEREALVYLTGFMVLIVLVILISIRDVANLIGG"
     gene            complement(5140..6288)
                     /gene="dxr"
                     /locus_tag="VIN32_05385"
     CDS             complement(5140..6288)
                     /gene="dxr"
                     /locus_tag="VIN32_05385"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014281113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEY8922045.1"
                     /translation="MTGVVLLGSTGSIGRQTLEVIAAHRDQFSVVGLAAGRRSDDFAA
                     QLGEWPGARAWCSDGRPADLAGDRWVADGLEELATLDDAEIVVVATTGMTALPAVLAA
                     LRGGRRVALANKETLVTGGHLVDRVLTEMGGDRLDRLRPIDSEHSAIWQCLAGERLDE
                     VARLILTASGGPFRVRLAGELEDVTPAQALAHPNWRMGPKITIDSATLVNKAFEAIEA
                     KWLYRLSYDRIQAVIHPQSVVHSLVEFTDGSFKAQLGVPDMRLPIQYALTYPRRLPSP
                     ARHAAPQEWGTLQFEALESGRFPAYDAVRAAADAGGNRGTILNAADEAAVAAFLAGTI
                     TFPRIAEVIGDAVERWGDSSEPDLEAIVILDGEVRAALSVELGLGGTD"
     gene            complement(6285..7184)
                     /locus_tag="VIN32_05390"
     CDS             complement(6285..7184)
                     /locus_tag="VIN32_05390"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEY8922046.1"
                     /translation="MLATRLISAGILAPIVVVVALVGEPWVSSLVGLLVFLAMVELIA
                     LLDAGGFAPPQVVALAAGLLVAGAGLVAANQALVGGALAEMLRATDPPGLATVAFAAA
                     VLVLGMVGFTRSEPRDGFLTWAITSFGVAYVGLLAPFIVVIAHLAPADGSATTPIGAL
                     NLHAGTAWMLLLLLVVWGYDSGAYLTGRWLGRRRLIDHISPSKTVEGLVGGLLAATVA
                     AGVGAWLIGLDAWQPLLIGPIVGLAAQAGDLAESMIKRAAGRKESGFLVPGHGGVLDR
                     IDSFLFAAPVLVGYALLIAGFSL"
     gene            complement(7192..7884)
                     /gene="uppS"
                     /locus_tag="VIN32_05395"
     CDS             complement(7192..7884)
                     /gene="uppS"
                     /locus_tag="VIN32_05395"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014240154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HEY8922047.1"
                     /translation="MPLREHPRHVAIIMDGNRRWAAARGLPAIAGHVQGVEAIRPIVR
                     VAPDKGIEVLSLYVFSRENWRRPDDEVTGLLGLIDGAVRQHTDELVAQGVRVRVIGRL
                     HEAPADIVRSIREAEERTRGGKKMALNIAFNYAGRAEIIDAARALVAAGVPAHEIDED
                     RFGEQLYTAGQPDPDLIVRTGGEQRTSNFLLWQGAYAELIFSETLWPDFAEADLDAAI
                     EEFARRQRRYGA"
     gene            complement(7890..8447)
                     /gene="frr"
                     /locus_tag="VIN32_05400"
     CDS             complement(7890..8447)
                     /gene="frr"
                     /locus_tag="VIN32_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEY8922048.1"
                     /translation="MTHPEILAAEPKMQRSLEAMERDFAGIRTGRASTALVERIQVEY
                     YGTPTPLNQIAGISTPDPHLIVIQPWDRSVLSAIERALIKSDIGLTPNVDGTVVRLSV
                     PPLTEDRRREMVKQVRKRAEEARVELRVHRREVADHLKKALRDGSLSEDEERRELEAL
                     QRMTDRFVELVEARGERKEAEVMEV"
     gene            complement(8444..9190)
                     /gene="pyrH"
                     /locus_tag="VIN32_05405"
     CDS             complement(8444..9190)
                     /gene="pyrH"
                     /locus_tag="VIN32_05405"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009627161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEY8922049.1"
                     /translation="MTDPSRDITRLPHRVLLKLSGEALMGDRQYGIDPDVVRGIAAQV
                     AEARRDGIEVAVVVGGGNIFRGVAAAASGMDRATADYMGMLATAMNGLALQDALEQAG
                     CATRVMSAIAMNEIAEPYIRRRAVRHLEKGRVVVLVAGTGNPYFTTDTAATLRAVELA
                     AEVILKATRVDGVYDADPEKHPNATRFSHIGYTELLASRLQALDATAVSLAMDNEMPI
                     VVFDMTQPGNILRAVRGETIGTRIGGEERP"
     gene            complement(9192..9809)
                     /gene="tsf"
                     /locus_tag="VIN32_05410"
     CDS             complement(9192..9809)
                     /gene="tsf"
                     /locus_tag="VIN32_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HEY8922050.1"
                     /translation="MIRPENVKRLREQTGAGIMDCKRALEHSGGDVDQAVAWLREKGL
                     STAAKKAGRAAREGVISSYIHHGARLGVLLELNCETDFVARTDDFQQLARELAMQVAG
                     LAPSWVSRDDVPAEVLNEQRQQFAAEAERDGRPADRVAEIVEGKLNKWLESVCLLEMP
                     FRDTEKRVSDLISEKIALLGENIRVARFARMAVGETAEREEQEAA"
     gene            complement(9850..10758)
                     /gene="rpsB"
                     /locus_tag="VIN32_05415"
     CDS             complement(9850..10758)
                     /gene="rpsB"
                     /locus_tag="VIN32_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012192480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEY8922051.1"
                     /translation="MAAVSMKQLLEAGVHFGHQTRRWNPKMRDFIFGERNGIHIIDLA
                     QTVTRLDDALEFVRETIRRGDSILFVGTKKQAQESVSQEAERSGQHYVNNRWLGGMLT
                     NFTTIKRRIQRLEALEARRDAGEFERLTKKEAGKLTEEIAKLNTALGGIRRMRRVPGA
                     LFIVDPHRESIAVLEARRLEIPIVAITDTNCDPDQIDWVIPANDDAIRSVRLICAKVA
                     DAALEAQAERQARLDEEFEQAEALPPVDDAALVADADYSAALASGEALVFEPEPEEDE
                     EEERRARARRAAEASEEETDEDPTLA"
     gene            11066..12142
                     /locus_tag="VIN32_05420"
     CDS             11066..12142
                     /locus_tag="VIN32_05420"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEY8922052.1"
                     /translation="MPHRTNLPLLRTAALVAIIAGAAVAVPVAATDRVVVVQAGDTLS
                     EIALKHGLTVAQLRDLNGIADPDRIYAGQRLRVSGMAASATPAPAARPTEVVHVVARG
                     ETLTGIARRYGSTIAGIVKANGIRNPSFLRVGQRLRIPGAASTHAAKPAAPAARAAAP
                     RMHVVARGETLTGIARHYGSTIAGIVKANGIRNPSFLHVGQRLTIPHAPATAPADKMP
                     ASISSLMAARGAIAAIIRTEAKSQHVPVALALAVAWQESGWRPRVVSAAGAIGVMQLT
                     PPTAEWIAITMLGHRVDLRDARSNVQAGVTLLRHYLDRYHGNRRLTLAAYYQGQTAAD
                     RHGMYRLTRPYIASILALEKLLGR"
     gene            complement(12155..12526)
                     /locus_tag="VIN32_05425"
     CDS             complement(12155..12526)
                     /locus_tag="VIN32_05425"
                     /inference="COORDINATES: protein motif:HMM:NF014119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEY8922053.1"
                     /translation="MPDPRHELGERAEEATAAWLEARGWTILARRWRCGEGELDLVAR
                     DPDRVLVGIEVKVRRTGRAGSPIESINRQRLGRLRAALGSFVSEASFSSENGLRIDLV
                     AMRPDGLGQWRLSHHRGIDAW"
     gene            complement(12569..13237)
                     /locus_tag="VIN32_05430"
     CDS             complement(12569..13237)
                     /locus_tag="VIN32_05430"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF000594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEY8922054.1"
                     /translation="MRHERELRLAGFRRIAGIDEVGRGSLAGPVVAAAVVLPDRHRIR
                     GLRDSKVLSRTRREALYELILDRAEGVGVGCMEVEVIDRINILQATKLAMRQALGHLS
                     PVPDHLVIDALSLREVDLPQRPIIDGDAISASIAAASIVAKVTRDRICDEFDERYPAY
                     GFARNKGYGTRRHVEALINEGPCEWHRRSFAPLRLMLAGEQLSLEMLLDAVVLTDEQV
                     DPGE"
     gene            complement(13311..13661)
                     /gene="rplS"
                     /locus_tag="VIN32_05435"
     CDS             complement(13311..13661)
                     /gene="rplS"
                     /locus_tag="VIN32_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEY8922055.1"
                     /translation="MDPIKELEREQLRSDVPAIAPGDTVKVSVKVVEGSRERIQIFEG
                     TVMRLRGGGLNQRVTVRRIASGVGVERTFMVHAPRVEKIEIVRHGVARRAQLYFLRDR
                     VGKSATLRERRPGR"
     gene            complement(13809..>14347)
                     /gene="trmD"
                     /locus_tag="VIN32_05440"
     CDS             complement(13809..>14347)
                     /gene="trmD"
                     /locus_tag="VIN32_05440"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015942267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEY8922056.1"
                     /translation="PLFAAIEPLRKDGAQVILLDPAGERLTDPLARELAAAGHLVLVC
                     GRYEGIDERARALADREVSIGDYVLTGGELPALVLIDAVGRLVPGVIEADSHESDSFA
                     DGLLEGPHYTRPEHFRDASVPPVLLSGHHGEIARWRRVEALRRTLARRPDLLATAPLT
                     DQDRADLRLLSGEEDPPA"
BASE COUNT         2198 a         5051 c         4792 g         2306 t
ORIGIN      
        1 cgacaggaag ggcggcggtg gatcgacgct caccacctcg gccttcttgc cgagccgctc
       61 ggccgccagg gctatggcga tcgcggcgcc gagcgtgtcg ccatcgggat tctcgtggca
      121 gaaggccgag atgcgctctg caccccgcag gagctcggca acgtcgagca ctagggctcc
      181 cccgcctcgt ccggcacagg gttcgtcgag ccctcatcga gctcctggag gatgcgcagg
      241 acccggtcgc ccgatgcggt cgagtcgtcc tcacggaccg tgagctccgg gacgtggcgc
      301 agcgagagtc gctcgccgag cttgcggcga agccacggag ttgcgacgcg cagtgcgtcc
      361 atggtgcgct cgcgctcgac gtcactccct aacacgctca cccagacccg tgcgtggcca
      421 aggtcgcggg ccgtttcgac ccgggtgatc gtggcgaagc cgaggcgtgg atccttcatc
      481 tcgcgctgca gcagatccat cagctcctgg cggatctggc tgtcgagccg atcggtccgc
      541 tgactcatcg gaggcgggtc acgcctgctc ggcaaccatc tggtagcact cgatcaggtc
      601 gccctcggcg atctcgatgt tcccgaggcc gatcccgcac tcgaaaccgg tcgcaacctc
      661 gcgcacatcg tccttgaagc gcttgagcga ctccacacga ccctcggcga cgacctggcc
      721 gccccggaag aggcgcacct ggccaccgcg gacgatgcgt ccgtcgacga cgtaggagcc
      781 ggcaacattc ccggcctttc cggccttgat gaccatccgg acctcggcgt ggccctcgat
      841 ctgctcgacc tgcttggggg tgagcatgcc gctcaacgcg gcgccaaggt catcggtcag
      901 cttgtagatg acgtcataaa gccgcacgtc aacgccaagc gcatcgaccg cgcgctgggc
      961 acccgggtcc agcttggtgt tgaagccgat gacgaccgct tcggaggcgc tggccagctg
     1021 cacgtcggat tcgttgatgt cgcccgctcc ctcgctgacg atgttgatcc gcacttcagc
     1081 ggtgttgagg cgctcgagtg catggcggat ggctccgagc gacccttgga cgtcggtctt
     1141 catgacgacg cgcagctcct tgacctccga ggcctggatc tgggcgctga tgtcctcgag
     1201 ggtgaaggct gccttcccat cgcctgacgt ggccgcctgc tcgaccgcct cggccatgtc
     1261 gcgcgccgtc ttctcgtcgg gcaccacgcg caggatgtct ccggcctcgg gcaggtcggt
     1321 tatgccgatg atctccaccg cggtagccgg ctccgcggtc ttgacgcgct tgccggcggc
     1381 gttctggagg gccctcacac gtccgaaggt gcgtccgacc gtcaccacgt cgccgatctt
     1441 cagcgtaccc gtctgcacca gcacggtggc gacgttgccg cggcccgtcg ccaccttcga
     1501 ttcgatgatg gtgccgatcg ccggtcgatt cggattggcc ttcaggtcct gcaggtcggc
     1561 gaccagcagg atcatctcca tcaggtcgtc gaggcccgtc ttctgcttgg cggataccgg
     1621 caccgcgatc acgtcgccgc cgaactcctc gatcaagacc ttgttgtccg ccagctcctg
     1681 cttcacgcgg tcgggattgg catcgggctt gtcgatcttg ttgatggcca cgatcatcgg
     1741 cacccgggcg gctcgaacgt ggtcgatcgc ctccttggtc tggggcatga cgccgtcgtc
     1801 ggcggccacc acgatgatgg cgatgtccgt gacctgggcg ccgcgggctc gcatggcggt
     1861 gaacgcctcg tggccgggcg tgtccaggaa gacgatccgc ttgccgttgt gctcgacctc
     1921 ggaggcgccg atgtgctggg tgatgccacc ccgttccgcg gttgcgacgc tcgtgctgcg
     1981 aatcgcgtcc aggaggctgg tcttcccatg gtcgacgtga cccatgatcg tcacgatcgg
     2041 ggcacgggtc acgagctccg cggcttcctc ctcggtgaag aggacgcgct tgccggcacc
     2101 ctcctcttcg ctgcccggcg ccacgatctc ctgctgtgac gccgcttcgg cggcagcgcg
     2161 ctgcgcgacg ccgcgctcca ccgtctcgat ccccagctcg ccggcgacga gcgaggcggt
     2221 gtcgaagtcg atcgactggt tgatcgtggc gaagataccg ttgcggatca gctccttgat
     2281 gacgtccgcg tggctgacgg tgagcgcggc cgcaaggtcc gtgaccgaga ccgacgacgg
     2341 caactcgacg ggcgggcctc cgcgcgggac aaccggctgg gtctcgaccg ccaggccgcc
     2401 ctcgggtccg ccgtcgcggc gccgcggcgg tcgcggacca cgccgcggct ggtatctacg
     2461 cggaggcatg tgtcacctcc gcgaggatct gttccggcac gaccgtgcca cccaacgcgc
     2521 gctggatccc ctctgccaga cggtggggat cgcggcaggc gggctggtcg cacaggtagg
     2581 tcccgcgtcc aggcgccttg ccaccgagat cgaccgtgac cgcgccatcc gcgcgacgga
     2641 cgacccgcgt catcgatcgc ttggggtgca cctcgcgaca gaccgcgcag gtccgctgtg
     2701 gctggtgctc aggcctccgc ttcggcatcg accggcgcct cctcgggggt tgcggcatcg
     2761 tccggggcac tgccatgcgc ggcggccgcg gtggcgacgc cggcgtctga acgaatatcg
     2821 atgcgccagc cggtcagctt ggcggcgagg cgggcgttct ggccctcctt gccgatcgcc
     2881 agcgagagct ggcgctccgg gacgacaaca ttggcgatct tgtgctcctc gtcgatccgc
     2941 acgttgagga cctcggctgg ggagagcgcc tcggccacga actgcgccgg atcctcgttc
     3001 cagagcacga cgtcgacctt ctcgccgttc agctccgcca cgatcgcctg gattcgcgct
     3061 ccgcgctggc ccaccgccgc gcccttggcg tcgacgccga cctggcggct ctcgaccgcg
     3121 accttggagc ggctgcccgc ctcgcgggcg attccgcgga tcacgacggt cccgttgtag
     3181 atctctggaa tctccatctc catcacgcgg cgcaggaatc cgcggtgggt tcggctcacg
     3241 acgagcaccg ggccgcgggt cgtgcgacgc acttccagga cgtagacctt cagatgctgg
     3301 ccgatgcggt agtgctcgcc ggggagctgc tcgctgacgg ccaggaccgc ctcgacgccc
     3361 ttgcccaggt cgaggacgac cgcctggtcc gtggtgcggt gcaccacgcc ggtcatgatc
     3421 tcgccctcgc ggctggcgta ctggctgtac accacgtcgc gctccgcctc gcggaggcgc
     3481 tgccggtaga cctggccggc ggtctgcgcg gcgatccggc cgagctgcgc ctgggtcacg
     3541 ttctcaggga cgcggacctt ggcgccgacg ccggtggttg ggtcgatctt ctgggcggcg
     3601 ctgacgagca tctgcagctc cggatcctcg acctcgtccg gcgtggcgac gacctcgtac
     3661 tcgcgaaaga cgctgatctc gccggattcc gggtcgaccc gcaccacgac gttctcgggg
     3721 gcgtcggcat tgcggcgata ggcggattcg atggcctgct ccagggtctc gatcagaatc
     3781 tcctggggaa cgcccttctc agcgttcagc tggagcaagg ctccgatgaa gtcccggttc
     3841 acatccgcac ctcgcccctg cggggcagaa acaaaaacgt gggactggcg aaccgaccca
     3901 cgttgcactc atgatcagta tcgatttggg accgctcgaa cggtccgaga aaccgcccgg
     3961 agtataccag ccgaggcagg ctcccgccag cccgcgagag gtcacccgac gatcagccgc
     4021 cgatcagatt tgcgacgtcg cggatcgaga tcaggatgac caggacaatc aggaccatga
     4081 agccggtcag gtagacgagc gcctctcgct cggccggcag gcgccggcgc cgcaccgcct
     4141 ccacgaccgt gacggcgatc ctgccgccat caaggggcgg gaagggaagg atgttgagga
     4201 ctgccaggtt gaccgacagg atgccgacga acacgagctg gctgacgagc ggggcctgga
     4261 ggacgcgacc cgtctctgac gcgatcccga tcggcccgac ggcggtgccc gcgtcaggag
     4321 ccagcccaag cagcccagcg acggctgagg ccagtccgcc aggaatcttt gcagcaaggt
     4381 ccgcggccga cacgaccccc ttccaggctg ccgcagctgg acttgcaacc gtgggcggga
     4441 cctcgacgaa tcgatctggc tcgtagctga accccaccgc gcccaggccc tttgcctcct
     4501 cggcggcgct cagcgagcgc ggcgtgacgg gaatcagggt ctcggccccg tcgcggcgga
     4561 cggccagctc cacgagatgg ccggccctcg agcgaacgta ggcggtgagg tcacgagata
     4621 ccgggaacgt gcggccatcg gccgccacga tcacgtcccc cacctcgagc ccggcggcga
     4681 tcgcggggct gccggcctgg atctcggtga cggtcaacgg cccgcgcccc tcggttcccg
     4741 gcaggctgag ggcgaccgcg aacagggtgg cggcgagcag gaagttcatg acgaccccgg
     4801 ccaccaggac cacgatccgc agccagatcg gcttccgatt gaaggccccc tccatcgcct
     4861 tgtcgatgga ggcctcggac aggccgcgct tgcgcatctt ctcggcctcg ccgtcgccat
     4921 cctcgccgag catcttgacg aagccaccga gagggatcca gttgagcgaa tagcgagtgc
     4981 cgtgccagac gatcgacccg attcgtggcg ggaacccgac cccgaactcc tgcaccgtga
     5041 tgcctgcacg ctttgccacg atgaagtggc caagctcgtg gacgaggacg aggacgaaca
     5101 gcaccgcgat gaaggcgagg atcgttccga acacgcccat cagtcggtgc ccccaagacc
     5161 cagctcgacc gagagcgcgg cacgcacctc gccgtcgagg atcacgatcg cttccaggtc
     5221 gggttcggag gagtcgcccc aacgctcgac ggcgtcgccg atgacttcgg cgatgcgcgg
     5281 gaaggtgatg gtgccggcga ggaaggcggc caccgcggcc tcgtcggccg cattcaggat
     5341 cgtcccgcga ttgccacccg cgtcggcagc ggcgcgaacc gcgtcataag ccggaaagcg
     5401 acccgattcg agagcctcga actgcagcgt cccccattcc tgcggcgcgg cgtggcgtgc
     5461 cggcgatggc aggcgccttg gataggtgag cgcgtactgg atcggaaggc gcatgtcggg
     5521 gacaccgagc tgtgccttga acgagccgtc ggtgaactct acgagcgagt gcaccacgct
     5581 ctgggggtgg atcacggcct gaatcctatc gtacgacagc cggtacaacc atttggcctc
     5641 gatcgcctcg aacgccttat tgacgagcgt ggcggagtcg atcgtgatct tggggcccat
     5701 ccgccagttc gggtgcgcca atgcctgggc aggtgtcacg tcctcgagct cgcccgcgag
     5761 tcgaacccga aaggggccgc ccgaggcagt gaggatcaga cgcgccacct catcgagccg
     5821 ttcccctgcg aggcactgcc agatcgccga gtgctcggag tcgattggcc gcagccggtc
     5881 gagccggtcg ccgcccatct ccgtcaggac gcggtcgacg aggtggccgc cggtgacgag
     5941 cgtctcctta ttggcgagcg ccacgcggcg gccgccgcgc aatgccgcaa ggaccgccgg
     6001 cagcgcggtc atgccggtgg tggcgacgac gacgatctcg gcgtcgtcca gggtcgcgag
     6061 ctcctcgagg ccgtcggcca cccatcggtc gcccgcgaga tcggcggggc gaccgtcgct
     6121 gcaccaggcg cgcgcgcctg gccactcgcc gagctgggcc gcgaagtcat cgctcctgcg
     6181 accggccgcc aggccgacca ccgagaactg atcgcggtgc gcagcgatca cctccagcgt
     6241 ctgccttccg atggatccgg tcgacccgag caggacgacc ccggtcacag gctgaagccg
     6301 gcgatcagga gcgcatagcc gaccagcacc ggcgccgcga aaaggaagga gtcgatccgg
     6361 tccaggacgc cgccgtgccc aggcaccagg aagccagact ccttgcggcc ggcggcgcgc
     6421 ttgatcatcg actcggccag gtcaccggct tgggcggcga gcccgacgat cgggccgatc
     6481 agcagcggct gccacgcgtc gagcccgatc agccaggcgc cgacgccggc agcaacggtc
     6541 gccgccagga gccccccgac cagcccctcg accgtctttg acgggctgat gtggtcgata
     6601 aggcgtcgcc gtcccagcca gcggccggtc aggtaggccc ccgagtcgta tccccacacc
     6661 acgagcagga gcaggagcat ccacgctgtc cctgcatgga ggttcagggc cccgatcggg
     6721 gtcgtggcag agccgtcggc cggcgccagg tgagcgatca ccacgatgaa gggagccagc
     6781 agcccgacgt aggcgacgcc gaaggaggtg atcgcccagg tcaggaagcc gtctcgtggc
     6841 tcggaccggg tgaagccaac catcccgagc accaggaccg ctgcggcgaa ggcgacggtc
     6901 gcgaggcccg gcgggtcagt ggcacgcagc atctcggcca gtgccccgcc caccagcgcc
     6961 tggttggcag cgaccaggcc ggcgccggcc acgagcaggc cggccgccag cgcgaccacc
     7021 tggggcgggg cgaagccacc ggcgtcgagc agcgcgatca gctcgaccat ggccaggaag
     7081 acgagcaggc cgaccaggga ggagacccag ggctcgccaa cgagcgcgac gacgacgacg
     7141 atcggcgcca ggatgccggc gctgatcaga cgggtggcga gcatcggctc atcatgcgcc
     7201 gtagcgccgc tgacgtcggg cgaactcctc gatcgcggca tcgagatcgg cctcggcgaa
     7261 atccggccac agggtctcgg agaagatgag ctccgcgtag gcgccctgcc agagcaggaa
     7321 gttgctcgtc cgctgctccc cgccggtgcg gactatcagg tccggatcgg gctgaccggc
     7381 ggtgtacagc tgttcgccga agcgatcctc gtcaatctca tgggcgggaa ctccggccgc
     7441 caccagggcc cgcgcggcgt cgatgatctc cgcgcgtccg gcgtagttga acgcgatgtt
     7501 gagcgccatc ttcttcccac ccctggtgcg ctcctccgcc tcgcggatcg agcgcacgat
     7561 gtcggccggg gcctcgtgca gacgaccgat gactcgaacg cgaacgccct gtgccaccag
     7621 ctcgtcggtg tgctgacgga ctgccccgtc gatcagcccc agcaggcccg tcacctcgtc
     7681 gtcggggcgg cgccagttct cgcggctgaa cacgtagagg gagaggacct cgattccctt
     7741 gtcgggcgcg acccgcacga tggggcggat cgcctcgacc ccctggacat ggccggcgat
     7801 ggccggcagg ccacgcgctg cggcccaacg gcggttgccg tccatgatga tcgcgacgtg
     7861 tcgcggatgc tcacggagcg gcaagcgcct cagacctcca tcacttccgc ttctttgcgc
     7921 tcgccacgcg cctccacgag ctccacgaag cggtcggtca tccgctgcag cgcctcgagc
     7981 tcgcggcgct cctcgtcctc agagaggctg ccgtcacgga gggccttctt gaggtgatcg
     8041 gcaacctcgc ggcggtggac gcgcagctcg acccgcgcct cctcggcgcg cttgcgcacc
     8101 tgcttcacca tctcccggcg acgatcctcg gtcagcggtg gcacgctgag gcgcaccacc
     8161 gttccgtcga cgttcggcgt gaggccgatg tcgctcttga tcagggcgcg ctcgatcgcg
     8221 gacaggaccg atcggtccca gggctggatc acgataaggt gcgggtcggg cgtgctgatc
     8281 ccggcgatct ggttcagcgg agtcggggtg ccgtagtact cgacctggat ccgctccacg
     8341 agagcggtgg acgcgcgtcc agtgcggatt cccgcaaagt cgcgttccat cgcctccaat
     8401 gagcgctgca tctttggctc cgccgccagg atctctggat gggtcacggc cgctcctcgc
     8461 ccccgatgcg ggttccaatc gtctcgccgc gcacggcacg caggatgttc cccggctgcg
     8521 tcatgtcgaa gaccacgatc ggcatctcat tgtccatcgc caggctcacg gcagtggcat
     8581 cgagcgcctg cagccggctg gcgaggagct cggtatagcc gatgtgggag aagcgcgtcg
     8641 cattcgggtg cttctcagga tcggcgtcgt agaccccgtc gacgcgagtt gccttcagga
     8701 tcacttcggc cgcgagctcg acggcccgca gtgttgccgc ggtatcggtc gtgaagtacg
     8761 ggttgccggt gcccgccacc agcaccacca cacggccctt ctcgaggtgc cgcacggcac
     8821 gacgccggat gtagggctcg gcgatctcgt tcatcgcgat ggcgctcatc acgcgtgtcg
     8881 cacagccagc ctgctcgagg gcgtcctgca gcgccaggcc attcatggcg gtcgcgagca
     8941 tgcccatgta gtctgccgtg gcccggtcca tgcccgaggc cgccgcggcg actccccgga
     9001 agatgttgcc gccgccgacc acgaccgcca cctcgatccc gtcgcgacgc gcctcggcaa
     9061 cctgggcggc gatgccacgc accacatcgg ggtcgatccc gtattggcga tcgcccatca
     9121 gcgcctcgcc cgagagcttg aggagcaccc ggtgcggaag cctcgtgatg tcgcgggagg
     9181 gatcggtcaa ttcaggcggc ctcctgctcc tcgcgttcag cggtctcgcc aacggccatg
     9241 cgcgcaaacc gcgcgacccg gatattctcg cccagcaggg caatcttctc gctgatgaga
     9301 tcgctgaccc gcttctccgt gtcgcggaat ggcatctcga gcaggcagac cgactccagc
     9361 cacttgttga gcttgccttc gacgatctcg gccacgcgat cggctggacg cccatcgcgt
     9421 tcggcctcgg cagcaaactg ctgccgctgc tcgttcagca cctccgccgg gacgtcgtcg
     9481 cggctcaccc agctgggggc caggccggca acctgcatcg ccagctctcg ggcgagctgc
     9541 tggaagtcgt cggtgcgcgc cacgaagtcc gtttcgcagt tgagctccag cagcactccc
     9601 agcctcgcac cgtggtggat gtagctggag atgaccccct cgcgcgcagc gcggccagcc
     9661 ttcttggcgg cagtcgagag gcccttctcg cggagccagg cgactgcctg atcgacgtcg
     9721 ccgccgctgt gttcgagtgc gcgcttgcaa tccatgatcc ccgcccccgt ctgctcacgc
     9781 aggcgcttca cgttctcggg tctgatcatc gcgtatcgtt cgttccttcc tgtcaggacc
     9841 gatgctgcct caggcaagcg tcggatcttc gtcggtctct tcctctgagg cctcggcggc
     9901 tcggcgggcg cgggctcgcc gctcctcttc ctcgtcctcc tcgggctcgg gctcgaacac
     9961 gagcgcctcg ccgctggcca gcgcggcact gtagtcggcg tcggccacga gggcggcgtc
    10021 gtccaccggc ggtagcgcct ctgcctgctc gaactcctcg tcgaggcggg cctgtcgctc
    10081 cgcctgcgcc tcgagcgcgg cgtcggccac cttggcgcag atcagccgca cggaccggat
    10141 tgcatcgtca ttggccggaa tcacccagtc gatctgatcg gggtcgcagt tggtgtcggt
    10201 gatggccacg atcgggatct cgaggcggcg cgcctccagc acggcgatcg actcgcgatg
    10261 gggatccacg atgaagaggg cgcccggaac gcgccgcatg cgacgaatcc cacccagggc
    10321 cgtgttcagc ttggcgatct cttcggtgag cttgccggcc tccttcttgg tgaggcgttc
    10381 gaactcgcct gcatcacgcc gcgcctcaag cgcctcgagg cgctggatgc gccgcttgat
    10441 ggtcgtgaag ttggtgagca ttccgcccag ccagcgattg ttgacatagt gctgaccgct
    10501 gcgttcggct tcctgggaaa ccgattcctg ggcctgcttc ttcgttccga cgaagaggat
    10561 cgagtcgccg cggcggatcg tctcgcgcac gaactcgagc gcatcatcca ggcgggtgac
    10621 cgtctgggcg aggtcgatga tgtggatccc gttgcgctct ccgaagatga agtcgcgcat
    10681 cttggggttc cagcgccggg tctggtgtcc gaagtggacg ccagcctcca gcagctgctt
    10741 catgctgacg gcagccaagg gtattcctcc ggttcttcct ccgctcgcct tcaactcccg
    10801 tcggtcgacc cgtcgcctgc gacgggcacc gttgaccgcg ggatcagcgg gcgtgtgtga
    10861 tgggatcttc ggccttcgtg cgtgcctgag cacgtggttg accgggtgac agtatagcag
    10921 cggcggcctc aacctccccg aggcccgtct ggaaacccct gcacacggta ccgaagtacc
    10981 ccaatccgca ccctctcgta ccttgaccgg gtggcgatgc ctgagacgat gcgcctgccc
    11041 gtcttcgccg aggagtcgca gttccatgcc acatcggacc aatctgcccc tgcttcgcac
    11101 cgctgccctg gtggcgatca tcgccggtgc cgccgtcgcc gtgccggtcg ccgccaccga
    11161 ccgcgtggtg gtcgtccagg caggcgacac cctcagcgag atcgcgctga agcacggcct
    11221 gaccgtagcg cagctccgcg acctcaacgg catcgccgat ccggatcgga tctacgcggg
    11281 ccagcgcctg agagtcagtg gaatggccgc cagcgcgacc ccggcgccgg ctgccaggcc
    11341 gacggaggtc gtccacgtgg tggcccgagg cgagacgctg acggggatag cgcgtcgcta
    11401 cgggtcgacg atcgcaggca tcgtcaaggc aaacgggatc cgcaacccct cgttcctgcg
    11461 cgtcggccag cggctccgca tcccgggcgc cgcatcgact cacgccgcaa agcccgccgc
    11521 accagccgcc agggctgccg cgccccggat gcatgtcgtc gcccggggcg agacgctgac
    11581 ggggatagcg cgtcactacg ggtcgacgat cgcgggcatc gtcaaggcaa acgggatccg
    11641 caacccctcg ttcctgcacg tcggccagcg gctcaccatc ccgcatgccc cggccaccgc
    11701 tccggctgac aagatgccgg ccagcatctc gtcgctgatg gcggcgcggg gagcgattgc
    11761 agccatcatc cgtaccgagg cgaagtcaca gcacgttccg gtcgccctgg cgcttgccgt
    11821 ggcctggcag gagtcaggct ggcgcccccg cgtagtcagc gccgcagggg ccattggcgt
    11881 catgcagctc acaccgccga ccgccgagtg gatcgccatc acgatgctcg ggcatcgcgt
    11941 cgacctgcgg gacgcccgct ccaacgtgca ggccggcgtc acattgctgc gtcattacct
    12001 cgaccgatac cacggcaacc gcaggttgac gctggccgcg tactaccagg gacagacggc
    12061 tgccgaccgc catggcatgt accggctcac ccgtccctac atcgcctcga tcctggcgct
    12121 ggagaagctg ctcggccgct gagagcgcct ccgctcacca tgcgtctatg ccccgatgat
    12181 ggctgagacg ccattgaccc agcccatcgg gacgcatcgc caccagatca atgcgtagcc
    12241 cgttctccga tgagaacgac gcctctgaca cgaagctgcc aagggccgcg cgcaggcgcc
    12301 cgaggcgctg ccggttgatg ctctcgatcg gactgccggc tcgacccgtc cggcgcacct
    12361 tcacctcgat cccgaccagg acacggtcgg gatcccgcgc gacgaggtcg agctcgccct
    12421 caccgcagcg ccaccggcgc gcgaggatcg tccagccgcg cgcctcaagc caggcggcgg
    12481 tcgcctcttc ggcacgttca ccaagctcgt gtcgcggatc gggcatcggg ttcgctcctc
    12541 gacgggaggc gagcagcgat ggtttcgcct actccccggg atccacctgc tcatcggtca
    12601 ggacaaccgc gtcgagcagc atctcgagcg agagctgctc gccggcaagc atcaggcgca
    12661 atggcgcgaa cgagcggcga tgccattcac acggcccctc gttgatcaac gcctcgacgt
    12721 ggcgtcgcgt gccgtatccc ttgttgcgtg cgaatccgta ggccgggtag cgctcgtcga
    12781 actcgtcgca gatgcggtcg cgggtcacct tggccacgat gcttgcggcg gcgatcgagg
    12841 cgctgatggc gtcgccgtcg atgattggtc gctggggcag gtcgacctcg cgcaggctca
    12901 gtgcatcgat caccaggtgg tcaggaacag gactcaggtg cccgagcgcc tgccgcatgg
    12961 ccagcttggt ggcctgcagg atgttgatgc ggtcgatcac ctcgacctcc atgcagccga
    13021 ccccgacccc ttccgcccgg tcgaggatca gctcgtacag cgcctcgcgc cgagtccggc
    13081 tgagcacctt gctgtcgcgc aggcccctga tccgatgccg gtccgggagg acgaccgccg
    13141 cggccaccac cggtccggcc agcgacccac ggcccacctc gtcgatcccg gcgatgcggc
    13201 gaaatccggc gagccgcagc tcgcgttcat ggcgcatgtg ggcgtcacga ggcggagatg
    13261 cgtgccgctt cgcacggcgc gttggtgtct ggaagaggcc cgccaccggc tcagcggccg
    13321 ggtcggcgct cgcgcagggt ggctgacttt ccgacccgat cgcgcaggaa gtagagctgg
    13381 gcacggcgag ccacgccgtg ccgcacgatc tcgatcttct cgacgcgcgg cgcatgcacc
    13441 atgaaggtgc gttcgacacc cacgccggat gcgatccggc gcaccgtcac gcgctggttc
    13501 agaccaccgc cgcgcagccg catgaccgtc ccctcgaaga tctgaattcg ctcgcggctc
    13561 ccctcgacca ccttgaccga caccttgacg gtgtcgccag gcgcgatcgc ggggacgtcg
    13621 ctgcggagct gctcgcgctc gagctccttg attggatcca tcctgcggga gacctcgtta
    13681 tcgcgccatt aactgcggat tatcggctcg gtggtgcgcc tgtgtcggcg cgaacgtgac
    13741 aatcgcgacc gaccagcggc cgcgatcggc ggagtatagc agcggtcctg gaaaggcgac
    13801 aaggccgctc aggcgggtgg gtcttcctcc ccggaaagta ggcgcaggtc cgcccgatcc
    13861 tgatccgtga gcggggccgt cgccagcagg tcgggacggc gggcgagcgt ccggcgcagc
    13921 gcctcgaccc gtcgccagcg agctatctcg ccgtgatgcc ccgagagcaa caccggcggg
    13981 accgaggcgt cccggaagtg ctcgggtcgg gtgtagtggg gtccctcgag gaggccgtcc
    14041 gcgaaggagt ccgactcgtg ggaatcggcc tcgatgacgc cggggaccag gcgaccgacg
    14101 gcatcgatca ggaccagcgc cgggagctcg ccgccggtca gcacgtagtc gccgatgctc
    14161 acctcgcggt cggcaagggc acgggcgcgc tcgtcaatcc cctcgtaccg accacatacg
    14221 agaaccaggt gacctgccgc ggcgagctcg cgggcgagcg gatcggtgag gcgctcgccg
    14281 gccgggtcga gcaggatcac ctgcgctccg tccttgcgca ggggctcgat cgccgcgaag
    14341 agcggct
//
LOCUS       DATCQR010000161         6475 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_55179, whole genome shotgun sequence.
ACCESSION   DATCQR010000161 DATCQR010000000
VERSION     DATCQR010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6475
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55179"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1962
                     /locus_tag="VIN32_05445"
     CDS             <1..1962
                     /locus_tag="VIN32_05445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8922057.1"
                     /translation="GELVDELLDLYTPRNLYALQTIANKIDAEFREPSLAAVFRLALA
                     ACLLPGSRLNGYPGRVASLRITGGHVRQPASRHQREVNVWRLFETAVHDVRTAVAALG
                     RDRRPARFAADLDELGGVGAANVLWIRCRPAVVGQYLPPEGVDLVLGSPPPAGSVDEM
                     SFEYLATAWLLGREAAETLRLEPLFGAAHGEGEPAEATAMRHAMASAAGALKPGGWCN
                     LLIEGGELDRVLAAAVAGAAADLELVDVVHRESARSGEGVALHFRKSSAEDRLRRVIE
                     ATPLQLGAEDGHLTYPELATAIDRAVTALLRRRGEPAGLMRVGAAVVVELQASGLLAR
                     LAASRGPAPEVADPGEEEQRTEERPERGPNLLATLLREELARDDHPSLVRIGDADRPL
                     WWLRRPELDDAPLADRVEWATFSILTTAGRLDEHSFLERIYVLFPGTEAPDEELVRAC
                     LAAYASVGEQGVLRTEEDLATRQDDHARLIGTLVDYGHRLGLRAWVGRREHDRSYAGA
                     TLLERLREDERRAYLPLIVRGPADTLAALDAIWYVRGRMAFTFDVEWTAMVGEPILRR
                     GCQIEPTDHQARFCVFPAERTELLRLKLDRSPWLRAEVARQNWHFLKWQHLEGLAARD
                     GASLDWLEPVLGLDPLIERGGEQLTMFGE"
     gene            complement(2028..2366)
                     /locus_tag="VIN32_05450"
     CDS             complement(2028..2366)
                     /locus_tag="VIN32_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013353610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEY8922058.1"
                     /translation="MTSETHATEHFRHAYTKDRGELLNRLRRIEGQVRGIARLVEDEA
                     YCLDILQQVEAMTAAADSVGLLLLEDHIDGCLTHAIETGQGEPYVDEVMTVVRRALGR
                     RSARPTGPRA"
     gene            2456..3184
                     /locus_tag="VIN32_05455"
     CDS             2456..3184
                     /locus_tag="VIN32_05455"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HEY8922059.1"
                     /translation="MPDLEREELIEPLDRALVIFSHPDDAEFSAAATIAHLTAAGARV
                     DYVVTTDGGKGTDDPGVTSAQLTATREAEQRAAADRLGVSEIVHLGYPDGYLTASLDL
                     RRDIVRQIRHFRPDLVIAQNPLRRQDGNPFIGHPDHLATGEATLAAVYPAARDRLNFP
                     ELIDDGLEPWKVRQVLVSGVEQPNLFLDVSETFDIGLQALMCHVSQIADPAAAEERVR
                     ERAAELGEPVGLPLANAFLSIIID"
     gene            3201..5093
                     /locus_tag="VIN32_05460"
     CDS             3201..5093
                     /locus_tag="VIN32_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8922060.1"
                     /translation="MMRGGHWGGGGEPNLRARPGSGPATFRRIVGFFRPYRRRLAWIA
                     LLIVITVSVGVVNPILLKLIIDNLTGPRDLGLLYLQAGLMIVLPIGTSLLGVWQSYLS
                     NVVGQRVMDDLRLALYSHLQWMPLRFFTETRTGEIQSRIGNDVNGVQSVVTDTAASLL
                     ANFATVATTVIAMLILDWRLTILSLGMLPVFAYITYRVGKVRRVVSGLTQQSLAEVSA
                     ITQESLSVSGVLLSKTFGQQQASIEKFGRESRRLGDLQIRQQMIGRWFFAMIGTFFSI
                     APAFVYLLAGSLIIGGDTNVSIGTIVAFTTLQSRLFFPLGQLLNVQVEIQGALALFDR
                     IFEYLEMEPEIRDAPDAIALDPATLRGDVRFRNVTFRYPSPPSAVAVPAEVEQAEAEG
                     RAVPIPVRPFGLDEIDFEAMPGQLVALVGPSGAGKTTTTYLIPRLYDVDEGAVEIDGI
                     DVRRIRLESLGREIGVVTQETYLFHSSVVENLRYAKPDATMEQIEAASRAAAMHDRVM
                     ELPDGYDTIVGERGYKLSGGEKQRVAIARVLLKDPRILLLDEATSALDTVSERLIQGA
                     LAGLMEGRTTIAIAHRLSTILRADLILVFDRGRIVERGTHDELVRHEGLYARLYAEQF
                     ASERPA"
     gene            5151..5699
                     /locus_tag="VIN32_05465"
     CDS             5151..5699
                     /locus_tag="VIN32_05465"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY8922061.1"
                     /translation="MITTTLNARTNVLSAASETDLLRDIVVHLQEERGGELWGLARHL
                     GLSPDEADDAVQETLLRLWSALREGAGIERPDAWAFRSLYRLAMDRHRWHRRVRLLTE
                     RLGAQPERRSGVDHADRMAIWEAVEGLPERQRLAVYLRYRADLPYEEIGAILGIAAPS
                     ARSHVSRALDALRVELGEEASR"
     gene            5696..6391
                     /locus_tag="VIN32_05470"
     CDS             5696..6391
                     /locus_tag="VIN32_05470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8922062.1"
                     /translation="MNDQDLERDLRTQRSPREEGYVPARLPMTLDEAPVASRGSRLPR
                     AVMLVGAGVAGALAVAIAAGLFSGSDPGVGSTESTSPSAAASTPVSGGGACGPQDVSL
                     TAEPWGGAAGSRGTVVTVALASGRAACMLGKGLAAEVADAKGSVLVTTGAAQAGGSVA
                     LVPGDSFTIGVTWSNWCEAAPAAPVTLALRMSGWDSFATVAVANGGLDPVPPCMGSAE
                     PTALSWSGLEPQQ"
BASE COUNT          944 a         2267 c         2256 g         1008 t
ORIGIN      
        1 ggcgagctgg tcgacgagct gctcgacctg tacacgccgc gcaacctgta cgcgctccag
       61 acgatcgcca acaagatcga cgccgagttt cgcgagccgt cgctcgccgc agtcttccgg
      121 cttgcgctgg cggcctgcct gctccccggc agccgcctca acggctaccc cggtcgagtc
      181 gcctcgctgc gcatcaccgg aggacatgtc cggcagcccg cctctcgtca ccagcgcgag
      241 gtgaacgtgt ggcgcctctt tgagacggcg gtgcatgacg tgcggacggc cgtggcagcg
      301 cttggccggg accgacgtcc cgcccgcttt gccgcagacc tcgacgagct gggcggcgtt
      361 ggcgccgcaa acgtgctctg gatccgatgc cggccggccg tggtcggaca gtacctgcca
      421 ccggaaggcg tcgacctggt cctcggctcg cccccgcccg cgggaagcgt tgacgagatg
      481 agcttcgagt atctcgccac ggcctggctg cttgggcggg aggcggcgga gacgctgcga
      541 ctcgagccac tcttcggagc agcgcacgga gagggcgagc cggccgaggc gactgccatg
      601 cggcacgcca tggcgtcggc cgccggggcg ctcaagccgg gtggctggtg caacctgctg
      661 atcgagggcg gcgagcttga tcgcgtgctc gcagccgccg tggccggggc ggccgcagac
      721 ctcgagctgg tcgacgtcgt ccaccgagag tcagcacgat ccggcgaggg ggtggcactc
      781 catttccgga agtcatcggc ggaggatcgc ctgcgccggg tcatcgaggc aacgccgctg
      841 cagctcggag cggaggacgg ccacctcacc tatcccgagc tggcgacggc gatcgaccgc
      901 gcggtgaccg cgctgctcag gcgtcgcggc gaaccggcgg gactgatgcg tgtcggcgcg
      961 gcggtcgtcg tcgagctgca ggcctccggg ctgctggcgc gccttgccgc atcccgcggg
     1021 cctgcgccgg aggtcgccga tccgggcgaa gaggagcagc gcaccgagga acgtcccgaa
     1081 cgcgggccga acctgctcgc caccttgctg cgcgaggagc tggcgcggga cgatcacccc
     1141 tcgctggtcc gcatcggcga cgcggaccgt cccctgtggt ggctgcgccg tcccgagctg
     1201 gacgacgcgc cccttgctga tcgggtcgag tgggcgacct tttccatcct gaccacggcc
     1261 ggccgcctcg acgagcattc cttcctggag cgcatctacg tcctcttccc cgggacggag
     1321 gcgccggacg aggagctggt gcgggcctgc ctggcggcct acgcatcggt cggcgagcag
     1381 ggggtgctgc gcaccgagga agacctggcg acgcgccagg atgaccacgc ccggctcatc
     1441 ggcacgctgg tcgactacgg ccaccgcctg ggcctccgtg cgtgggtcgg tcgccgtgag
     1501 cacgaccgct cctacgccgg cgcgacgctg ctcgaacgcc tccgcgagga cgagcgccgc
     1561 gcgtacctgc cgctgatcgt gcgcggcccg gcggacaccc tggctgcgct ggacgcgatc
     1621 tggtacgtac gcggccggat ggcgttcacg ttcgacgtcg aatggaccgc catggtgggg
     1681 gagccgatcc tgcgtcgcgg atgccagatc gagccgaccg accaccaggc gcgcttctgc
     1741 gtcttcccgg ccgagcggac cgagctgctc cgcctcaagc tggaccgatc accgtggctg
     1801 cgggccgagg ttgcgcgcca gaactggcac ttcctcaagt ggcagcacct cgaggggctc
     1861 gccgctcgcg atggtgcaag cctcgactgg ctggagccgg tgctcgggct agatcccctg
     1921 atcgagcgtg gcggggaaca gctcacgatg ttcggcgagt agttggcgcc ccgtactaga
     1981 ctcgaccgtg ggagtgtcgt ggttcccccc cgcggcactc ccatccttca tgctcgaggc
     2041 cccgtcgggc gcgccgatcg cctgcccagc gcgcgccgca ccacggtcat cacctcgtcc
     2101 acgtagggct ctccctggcc ggtctcgatg gcgtgcgtca ggcagccgtc gatgtggtcc
     2161 tcgaggagca gcaacccgac ggaatcggca gctgccgtca tggcctccac ctgctgcagg
     2221 atgtccaggc aatacgcctc gtcttcgacg agccgggcga tgccgcgcac ctggccctcg
     2281 atgcggcgca gccggttcag cagctcgccg cgatccttgg tataggcgtg gcgaaaatgc
     2341 tcggtggcgt gcgtctcgct ggtcatactc gggggcagta tgcggactgg cagttcgcga
     2401 ggtcaatccg gctcgcggag ccccgcgccg tttccgcgct acgatgcggc cagcaatgcc
     2461 cgaccttgaa cgcgaagagc tgatcgaacc gctcgacaga gcgctggtga tcttcagcca
     2521 tcccgacgac gcggagttca gcgccgccgc gaccatcgcc cacctgaccg ctgccggcgc
     2581 gcgcgtggat tacgtggtga ccactgacgg cggcaagggc accgacgacc ccggggtcac
     2641 ctcggcccag ctgaccgcga cgcgcgaggc ggagcagcgg gcggccgcag atcggctcgg
     2701 cgtgtcggag atcgttcatc tcggctaccc ggacgggtac ctgacggcga gcctcgacct
     2761 gcggcgcgac atcgtgcggc agatccgcca cttccggccc gacctggtga tcgcccagaa
     2821 cccgctgcgg cgacaggatg gaaacccgtt catcggtcac cccgaccacc tcgctaccgg
     2881 agaggcaacc ctcgcggctg tctatcccgc tgcgcgcgat cgcctcaact tccctgagct
     2941 gatcgacgac ggactcgagc cctggaaggt gcgccaggtg ctggtcagcg gggtcgagca
     3001 gccgaatctc ttcctcgatg tctccgagac gttcgatatc ggcctccagg cactgatgtg
     3061 ccacgtcagc cagatcgcgg atccggccgc cgccgaggag cgcgtacgag agcgggcggc
     3121 cgagctcggg gagccggtcg gcctgccgct ggccaacgcc ttcctctcga tcatcatcga
     3181 ctgatgggag ccagacgccg atgatgcgcg gcggtcactg gggaggcggc ggggagccga
     3241 acctccgcgc ccggccgggg agcggcccgg ccaccttccg gcggatcgtc ggcttcttcc
     3301 ggccctaccg ccgtcgactg gcgtggatcg cgctcctgat cgtgatcacg gtcagcgtcg
     3361 gcgtcgtgaa cccgatcctc ctcaagctga tcatcgacaa cctgactggg cccagggacc
     3421 tgggactcct ctacctgcag gccgggctga tgatcgtgct cccaatcggc accagcctgc
     3481 tcggggtctg gcagtcgtac ctgtcgaacg tggtcggcca gcgcgtcatg gacgacctgc
     3541 gactggccct ctacagccac ctgcagtgga tgccgctccg cttcttcacc gagacgcgga
     3601 ccggcgagat ccagagccgc atcggcaacg acgtcaacgg cgtgcagtcg gtggtgaccg
     3661 atacggccgc ctcgctgctc gccaacttcg cgacggtggc caccacggtc atcgcaatgc
     3721 tgatcctcga ctggcggctg acgatcctca gccttggcat gctgcccgtg ttcgcctaca
     3781 tcacctaccg ggtcggcaag gtgcggcgcg tggtgagcgg cctcacgcag cagtcattgg
     3841 ccgaggtgag cgccatcacc caggagtcgc tctccgtctc gggggtcctg ctctcgaaga
     3901 ccttcggtca gcagcaggcg agcatcgaga agttcgggcg ggagtcgcga cgcctcggcg
     3961 acctgcagat ccggcagcag atgatcgggc gctggttctt cgccatgatc ggcaccttct
     4021 tctcgatcgc gcccgcgttc gtctacctgc tggcgggctc cctcatcatc ggcggcgaca
     4081 ccaacgtcag catcggcacc atcgtcgcct tcaccacgct ccagagccgg ctcttcttcc
     4141 cccttgggca gctgctgaac gtgcaggtcg agatccaggg cgccctcgcc ctcttcgacc
     4201 ggatcttcga gtacctcgag atggagccgg agatccgcga cgcccccgat gcgatcgccc
     4261 tcgaccccgc cacgctccgc ggagacgtga ggttccggaa cgtcaccttc cgctatccgt
     4321 caccaccctc ggcagtggcg gtcccggccg aagtcgagca ggccgaggcg gagggccgtg
     4381 ccgttccgat cccggtccga ccgttcgggc tggacgagat cgatttcgag gcgatgcccg
     4441 gccagctggt cgccctggtc ggtccctccg gtgcgggaaa gaccaccacc acctacctga
     4501 tcccgcgact ctatgacgtc gacgaaggtg ccgtcgagat cgatggcatc gacgtgcgca
     4561 ggatccgcct cgagtccctg gggcgcgaga tcggcgtggt gacccaggag acctacctct
     4621 tccattcatc ggtcgtcgag aacctgcgat acgcaaagcc ggacgcgacc atggagcaga
     4681 tcgaggccgc atcccgggcg gcggcgatgc atgaccgcgt gatggagcta cccgatggct
     4741 atgacaccat cgtcggggag cgcggctaca agctctccgg cggcgagaag cagcgcgtgg
     4801 cgatcgcgcg cgtcctgctc aaggaccccc gcatcctgct ccttgacgag gcgaccagcg
     4861 cactcgacac ggtcagcgaa cggctcatcc agggtgcgct ggcgggcctg atggaggggc
     4921 gcaccacgat cgccatcgcg caccgcctgt cgaccatcct gcgggccgac ctgatcctcg
     4981 tcttcgatcg cgggcgcatc gtcgagcgcg ggacccacga cgagctggtg cgccacgagg
     5041 gcctctacgc gcgtctgtac gccgagcagt tcgccagcga gcggcccgcc tgacgcagaa
     5101 ggctgtgccg cggctttcaa cgttccggcc ctgtcgggcg tcggatgggc atgatcacca
     5161 ccaccctgaa cgcgcggacg aacgtgctct cggcagcatc ggagaccgat cttttgcgtg
     5221 acatcgtcgt acacctgcag gaggagaggg gcggcgagct gtgggggctt gcccgccacc
     5281 tcggcctctc gcctgacgag gcggacgacg cggtgcagga gacgctcctg cgcctctggt
     5341 cagcacttcg cgagggggcc ggcatcgagc gcccggacgc ctgggcgttc cgctcgctct
     5401 accgactcgc gatggaccgc catcgctggc atcgccgcgt ccgtctcctg accgagcggc
     5461 tgggcgcgca gcccgagcgt cgctccggcg tcgaccacgc cgatcggatg gcgatctggg
     5521 aggcggttga ggggttgccg gagaggcagc gcctggcggt ctaccttcga tatcgggcag
     5581 atctgccgta cgaggagatc ggggcgatcc tggggatcgc cgcaccctcg gcgcgcagcc
     5641 atgtgagccg tgcgctcgac gcccttcgcg tcgagcttgg cgaggaggca tcgcgatgaa
     5701 cgaccaggac ctggagcggg acctgcgcac ccagcgcagc ccgcgcgaag agggatacgt
     5761 gccggcgcgg ctcccgatga cgctggatga agccccggtt gccagccgag gctcacggct
     5821 gccgcgggcc gtcatgctcg tgggcgcggg cgtggccggt gcgctggcgg tcgccatcgc
     5881 cgcaggcctc ttctccggct ctgacccggg cgttggctcg accgaatcga cgtcgccgtc
     5941 ggccgcggcc tcgacgccgg tatcgggcgg cggcgcatgc gggccgcagg acgtgagcct
     6001 tacggcggag ccgtgggggg gcgcagcggg ctcacgcgga acggtcgtga cggtggcgct
     6061 ggcgagcgga cgcgcggcct gcatgctcgg caaggggctg gcggccgagg ttgccgatgc
     6121 caagggaagc gtgctggtca cgacgggggc ggcccaggcc ggggggtcgg tcgcgcttgt
     6181 gcccggggac tcgttcacca tcggggtcac ctggagcaac tggtgcgaag ccgccccggc
     6241 ggcgccggtg actctcgcgt tgaggatgtc cggctgggat tcgttcgcga cggtagccgt
     6301 cgcgaacggt ggcctcgacc cggtgccacc ctgcatgggc agcgctgagc cgaccgccct
     6361 cagctggtcc ggtctcgagc cgcagcagta gcggtgaagg cggcgggctc gtcgaggtga
     6421 cagacttggt cgcgtgacca accgccttgc ctccgagacg agcccgtacc tcctc
//
LOCUS       DATCQR010000162         2073 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_101439, whole genome shotgun sequence.
ACCESSION   DATCQR010000162 DATCQR010000000
VERSION     DATCQR010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101439"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2073
                     /locus_tag="VIN32_05475"
     CDS             <1..>2073
                     /locus_tag="VIN32_05475"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY8922063.1"
                     /translation="DALAGRAPSLPSEALQADLVRLEQGDVGEAAETWAALLTAGPAA
                     EHIPASAHVVLTDPFELRAIAADLDTQATERRAGLIASGELPDAWPLPYEALATLGVL
                     AARAAEVLDEGGADAGYATAPSLPGRADRIGSWLAQLASGKRLVVTTDQASRVGELLE
                     EEGRAVAPVAELTETPPPGGIGLVHGSLSGGFTHGPSGLQLLTDRELFGATRVRRLTP
                     SKRSMTRDLIGKLAVGDAVVHIDHGIARYAGMTQREYGGATKEYLQLDFAGDDKIFLP
                     ADQIGRITRYSGGPAPTLSRLGGTDWERTKGRVRRAVAELAEDLLAIYAARASAPGFS
                     FSPDTTWQRELEEAFPYTETKDQERTIQEVKADMLRRRPMDRLVCGDVGYGKTEVALR
                     AAFKAVQDGKQVAVLVPTTVLAQQHLGTFRRRLAPFPVRVEMLSRFVGKAEQGRIVDA
                     TAGGEVDILIGTHRILSRDIAFADLGLLVVDEEQRFGVGHKERIKSMRREVDVLTLSA
                     TPIPRTLHLSLVGIRDLSVIETPPEARLPILTRIAEDDDGLVRDAINRELDRGGQVFY
                     VHNRVETIEGAAERVRRLVPRAKVAIGHGQMAEGMLERVMLDYDAGRFDVLVCTTIIE
                     SGLDIPNANTIVIARADTLGLAQLYQLRGRVGRSDRRAYAYLLHRRGQPLTAVARKRL
                     HAIFSASD"
BASE COUNT          305 a          732 c          748 g          288 t
ORIGIN      
        1 gggacgccct ggccggccgg gcgccgagcc tcccctcgga ggcgctgcag gccgacctcg
       61 tgcgactcga gcagggcgac gttggggagg cggccgaaac gtgggcagcc cttctcaccg
      121 ccggtccggc ggccgagcac atccccgcca gcgcacacgt cgtgctcacc gacccgttcg
      181 agctgcgcgc gatcgcggcc gacctggaca cgcaggccac cgagcgtcgc gcgggcctca
      241 ttgcgtcggg cgagcttccg gatgcctggc cccttcccta cgaggctttg gccacgcttg
      301 gcgtcctggc ggcgcgcgcg gccgaggtcc tcgacgaggg cggcgccgat gccggctatg
      361 cgaccgcacc gtcgctgccg ggacgggccg accgcatcgg ctcgtggctg gcccagctgg
      421 cgtcgggaaa gcggctcgtg gtcacgaccg accaggccag tcgcgtcggc gagctgctcg
      481 aggaggaggg ccgtgccgtc gcgccggttg cggagctgac cgagaccccg ccaccaggcg
      541 gcatcggcct tgtccatggc tcgctctccg gcggtttcac gcacggccca tcaggcctgc
      601 agctgctcac cgaccgcgag ctgttcggcg ccacccgggt tcgacgcctg acgccgtcga
      661 agcgctccat gacccgcgac ctgatcggga agctggcggt cggcgacgcg gtggtccaca
      721 tcgaccatgg cattgcccgc tacgcgggga tgacgcagcg cgagtacggc ggcgccacca
      781 aggagtacct ccagctcgac ttcgccggcg acgacaagat cttcttgccg gcggaccaga
      841 tcgggcgcat cacgcgctac agcggcggcc cggcaccgac cctctctcgc ctgggcggga
      901 ccgattggga gcgcaccaag ggacgcgtgc gtcgcgccgt ggcggagctc gcggaggacc
      961 tgctggccat ctatgcggcg cgggcatcgg cgccgggctt cagcttctcg ccggacacga
     1021 cctggcagcg cgagctggag gaggcgttcc cgtacaccga gacgaaggac caggaacgga
     1081 ccatccagga ggtcaaggcc gacatgcttc gccgccggcc gatggaccgg ctggtgtgcg
     1141 gcgacgtcgg ctacggcaag accgaggtgg cgctgcgcgc cgcattcaag gccgtgcagg
     1201 acggcaagca ggtggcggtc ctcgtcccga ccaccgtgct ggcgcagcag cacttgggca
     1261 ccttccgccg ccgcctggcg ccattcccgg tacgggtcga gatgctgagc cggttcgtcg
     1321 gcaaggccga gcagggacgg atcgtggatg ccaccgccgg gggagaggtc gacatcctga
     1381 tcgggacgca tcggatcctc agccgggaca tcgcattcgc cgacctcggg ctgctggtcg
     1441 tcgatgagga gcaacgcttc ggggtcggcc acaaggagcg gatcaagtcg atgcggcgcg
     1501 aggtcgacgt gctcacgctc tcggccacgc cgatcccgcg cacgctgcac ctctccctgg
     1561 tcgggatccg cgacctgtcg gtgatcgaga cgccgccgga ggcgaggctg ccgatcctga
     1621 cgcgcatcgc ggaggacgat gacggcctgg tgcgggacgc catcaaccgc gagctggacc
     1681 gtggcgggca ggtcttctac gtccacaacc gggtcgagac gatcgaaggg gctgccgagc
     1741 gggtgcggcg cctggtgccg cgggccaagg tcgccatcgg ccatggacag atggcggagg
     1801 ggatgctcga acgggtgatg ctcgactacg acgccggccg cttcgacgtc ctggtgtgca
     1861 cgaccatcat cgagtcgggg ctggacatcc cgaacgcgaa cacgatcgtc atcgcccgcg
     1921 ccgatacgct cgggctggcg cagctctacc agctgcgcgg tcgggtcggc cgatcggatc
     1981 gccgtgccta cgcgtacctg ctgcaccggc gggggcagcc gctcaccgcc gtcgcccgca
     2041 agcggctgca cgcgatcttc tccgcctccg acc
//
LOCUS       DATCQR010000163         1807 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_210708, whole genome shotgun sequence.
ACCESSION   DATCQR010000163 DATCQR010000000
VERSION     DATCQR010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1807
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210708"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..540)
                     /locus_tag="VIN32_05480"
     CDS             complement(<1..540)
                     /locus_tag="VIN32_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009657040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HEY8922064.1"
                     /translation="MNRIHRPESYLVPMVVENTGRGERAFDIYSRLLKDRIVFIGTPI
                     DDQVANLVVAQLLFLQSEDPDKEISLYVNSPGGGVTAGLAIYDTMQYIRPAVSTICIG
                     MAYSMAAVLLAGGAHGMRYALPHANILIHQPWGGMQGQATDIQIHAKEILRTREQLNQ
                     ILARHTGQKIEKVTLDTERD"
     gene            complement(689..>1807)
                     /gene="tig"
                     /locus_tag="VIN32_05485"
     CDS             complement(689..>1807)
                     /gene="tig"
                     /locus_tag="VIN32_05485"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEY8922065.1"
                     /translation="PAVVREEVVEVMVPEVMRQVLQEGAIDPIESPDVEVLELERGRP
                     ARLKATVSVMPEVKLGEVPKLEEPINSIEVTDEMVDRRLEDLREPLAEITPVEREARM
                     GDVVVIDVEVDVDGAVVESETRKAMEAELKEGVLLPELLEVIPGSFVGDTRSVTVKFP
                     EDYSEPRLAGKDATIKVTVQGVKEKVLPALDDEMAKQLSNGEHENVESFRKSTRAALE
                     ESANEMAKLDREQALVKGLVESSSVDVPVALVDRELTSQLESMERTLSRQGLKLERYF
                     EYMGKNINQWVEEERPAAEQRLKVDLVLGEYAKRQDLEPSDEDVASFLEEQAGQDDEL
                     KGQVEELKRSESARRYFASRLRRRRVLDRLTAEAPTSK"
BASE COUNT          266 a          653 c          534 g          354 t
ORIGIN      
        1 gtcgcgctcg gtgtcgagcg tcaccttctc gatcttctga cccgtgtgcc gagccaggat
       61 ctggttcagc tgctcgcggg tgcgaaggat ctccttggcg tggatctgga tgtcggtcgc
      121 ctggccctgc atcccgcccc acggctggtg gatgaggatg ttggcgtgag gcagcgcgta
      181 gcgcatgccg tgcgcgccgc ccgccagcag gactgcggcc atcgagtagg ccatgccgat
      241 gcagatcgtc gacaccgccg gccggatgta ctgcatcgtg tcgtagatcg ccagccccgc
      301 ggtgaccccg ccgcccgggc tgttgacgta gagcgagatc tccttgtcgg ggtcctcgga
      361 ctgcaggaac agaagctgcg cgaccaccag gttcgccacc tggtcgtcga tgggcgtacc
      421 gatgaaaacg atgcggtcct tcagcagccg ggagtagatg tcgaaggcgc gctcgccgcg
      481 ccccgtgttc tcgaccacca tcggcaccag gtagctctcg gggcgatgta tccggttcat
      541 atcgtgcaca acgtcattct agacacgcct ccccccggtt ccgtcaagtc gagagggggg
      601 aattcccccc tctggtgcgg cgggcccggc gggagccggc tcccggcgca acccccctgt
      661 cagaacgggt attgctgctt ggggcgcctt acttggaggt cggcgcttca gcggtcaggc
      721 gatccagcac gcgccgccgc cttagccgtg aggcgaaata ccgccgcgcg ctctcgcttc
      781 tcttcagctc ctcgacctgg cccttcagct catcgtcctg gcccgcctgc tcttcgagga
      841 agctcgcgac gtcctcatcc gacggctcga ggtcctgccg cttcgcatac tcgcccagca
      901 cgaggtcgac cttgaggcgc tgctcggcgg cagggcgctc ctcctcgacc cactggttga
      961 tgttcttgcc catatactcg aagtacctct cgagcttcaa cccctggcgg ctcagcgtcc
     1021 gctccatgga ttcgagctgg ctcgtcagct cacgatccac tagcgccacc ggcacgtcga
     1081 cgctagacga ctcgacaaga cccttgacga gggcttgctc gcggtcgagc ttcgccatct
     1141 cgttggcgga ctcctccagc gcagcgcgcg tcgacttgcg gaatgactcc acgttttcgt
     1201 gctcgccgtt gctcagctgc ttggccatct catcgtcgag ggcggggagc accttctcct
     1261 tgacgccctg gactgtgacc ttgatcgtcg cgtccttgcc ggccagccga ggttcgctgt
     1321 agtcctcggg gaatttcacg gtcaccgatc gcgtgtcgcc gacgaatgag ccgggaatca
     1381 cctcgagcag ctcgggcagc aagacgcctt ctttcagctc cgcctccatc gccttgcgcg
     1441 tctccgactc gaccaccgcg ccgtccacat cgacctcgac gtcgatcacc accacgtcgc
     1501 ccatgcgagc ctcacgctcc accggtgtta tctccgccag cggctcgcga aggtcctcga
     1561 ggcggcgatc gaccatctcg tccgtgacct cgatcgaatt gatcggctcc tcaagcttcg
     1621 gcacctcgcc gagcttcacc tcgggcatca cgctgacggt cgccttcagc cgcgccggcc
     1681 tgccgcgctc cagctcgagc acctcgacgt cggggctttc gatcggatcg atcgcgccct
     1741 cctgaagcac ctggcgcatg acctccggca ccatcacctc gaccacctct tccctgacca
     1801 ccgcggg
//
LOCUS       DATCQR010000164         2640 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_86878, whole genome shotgun sequence.
ACCESSION   DATCQR010000164 DATCQR010000000
VERSION     DATCQR010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2640
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86878"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..690)
                     /locus_tag="VIN32_05490"
     CDS             complement(<1..690)
                     /locus_tag="VIN32_05490"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY8922066.1"
                     /translation="MAELRHYRYFVEIAKRGSFTAAAEGLNITQSTLSEQILQLEREC
                     GSTLFNRAHSGITLTTAGDYLLPRAQKLLADAAETREGLAGFRTGYHDRLRMASVLGP
                     LQSWLPDALAEFAQLHPHVQLQVRFADAVNEILTQVAAGERDLGVVSLRPSAPARSRH
                     DALEQTVILDEDLVVLAPAGHSLGQLTHVSQADLRETPLILFPPDYYIRQITEDWTRR
                     GGYAPIVAAELG"
     gene            753..1634
                     /locus_tag="VIN32_05495"
     CDS             753..1634
                     /locus_tag="VIN32_05495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8922067.1"
                     /translation="MSKFRIVPHNRLQEWVAEEKGYFADEGLDYEFVSDVDFRAAGAQ
                     PAVDGGPDEVLYGAFDSMRQGRQCEVSGACHWAINAASATQHGRMWGRAYAVAPGGIY
                     VPPESAVLRPEDLAGVEIGVGLRSGSHFATVQGLEGILGPADVRLRFVGIPADRLRLM
                     VDRRLEAANLFGPPTYVLEQQGFRKILDTTFIVAFLVTGEAIPADVERYVRALERAQR
                     DIDLEPHRYKHYYLRELAPEFHSLVDVRAFGLGERIVVAPYTRDSFESTYRWVAKSGI
                     LPLEDLSELTYKEAVLA"
BASE COUNT          426 a          888 c          889 g          437 t
ORIGIN      
        1 gcccagctcc gccgccacga tcggtgcgta cccgcctcgg cgcgtccagt cctccgtgat
       61 ctgccttatg tagtagtcgg gcgggaacag gatcagcggc gtctcccgga ggtcggcctg
      121 gctgacgtgc gtgagctggc cgagcgagtg gccggccggg gccaggacca ccaggtcctc
      181 atccaggatg accgtctgct cgagagcgtc gtggcgcgac cgggcgggcg ccgaggggcg
      241 caggctgacg acccccaggt cgcgctcacc ggcggcgacc tgggtcagga tctcgttcac
      301 cgcgtcggcg aaccgaacct gcagctggac gtgcgggtgg agctgagcga actcggcgag
      361 cgcgtccggc aaccaggact gcagcgggcc caggacggag gccatccgaa ggcggtcgtg
      421 gtagccggtg cgaaaacccg ccagaccctc acgcgtctcc gccgcgtcgg ccagcagctt
      481 ttgtgcgcgc ggcagcaggt agtcaccggc cgttgtcagc gtgatgccgg agtgggcgcg
      541 gttgaagagc gtggagccgc actcccgctc cagctggagg atctgctcgc tcagcgtcga
      601 ctgggtgatg ttgagtcctt cggccgcggc cgtgaaggag ccgcgctttg cgatctcgac
      661 gaaatagcgg tagtggcgca gctcggcgat ggctcagaac actagctgaa ccaacccgtg
      721 cgaccggtgt catgattcga acggaggtgg acatgtcgaa gttcaggatc gtgccgcaca
      781 accggctgca ggagtgggtg gcggaagaga agggctactt cgcggacgag ggactcgact
      841 atgagttcgt ctccgacgtc gacttccgcg ccgcgggggc gcagcccgcc gtcgacggtg
      901 gtcccgacga agtgctctat ggcgccttcg attcgatgcg tcagggccgc cagtgcgagg
      961 tcagcggcgc gtgccactgg gcgatcaacg ctgcctcggc cacacagcac gggcgcatgt
     1021 ggggccgcgc ctacgcggta gcccccggtg gcatctacgt gcccccggag tcggcggtcc
     1081 tgcgccctga ggacctggcc ggggtcgaga tcggggtcgg cctgcgttcg ggcagccatt
     1141 tcgcgacggt gcagggcctg gagggaatcc tcggtcccgc ggacgtccgg ctgcgcttcg
     1201 tcggcatccc ggccgaccgg ctgcggctga tggttgaccg ccgtctcgag gcggccaacc
     1261 tcttcggccc tcccacctac gtgctcgagc agcagggctt ccgaaagatc ctcgacacca
     1321 cgttcatcgt cgccttcctg gtgaccggtg aagcgatccc ggcggacgtc gagcgctacg
     1381 tgcgtgcgct ggagcgcgcg cagcgcgaca tcgatctcga gccccatcgg tacaagcact
     1441 actacctgcg cgaactcgcg cccgagttcc actcgctggt cgacgtgcgc gccttcgggc
     1501 tgggcgagcg gatcgtcgtc gcgccctaca cccgcgactc attcgaatcg acctaccgct
     1561 gggttgcgaa gagcgggatc ctacccctcg aggacctgag cgagctgacc tacaaagagg
     1621 ccgtcctcgc ctgagtgcga gcgtgagaca ggaaggtccg taggagaagg aagcgcaggc
     1681 cggtggcgac ggcgttggcg gctgtgctca ccgccgcccg gggccttgcg ccggagcacc
     1741 tgtaggaggg caaaactcca tccggcacga tcggcgacgc tcctgcgcgc gatcctgacc
     1801 gagctcgtcc gctactacaa ccacgatcgc ccccatcgca ccctcggcct ccagcctccg
     1861 gctccacggc cgcagggtca ttacggcgcc gtcactgtcc gtcctgtcct cggcggactc
     1921 caccatgtct atcagcgagc agcttgacca ggaccgactt ttgccgccct acagcccgca
     1981 ctgtcacccg ctttacactt cggccgtgca cccatcggac gccctcccgg ccagcaaggc
     2041 catcctgcga cgcggcgagc gctactgggt cagcgtctac cacgagcttg cctgccgcca
     2101 cgagcatctg agctccctac aggctgtgca ctgttgccgc cacaggcggc gcgacgaggc
     2161 atacgaccgg gcggcgagag atccggcggg cccagtcaaa ccgctcgaat tcagggagag
     2221 gcccaaacga tagccgcgcg cccttgaggt gggctctgtt gcctagccac tgacagcaac
     2281 gccgacggac ttgcgcgtct gccggcgaat cttggcggaa ctaaggctcg aaatccgtac
     2341 tccgggcgga cgtcggcggc tctcggcaga cgccgtgagg attcctgtaa aaccgccgcg
     2401 aaagctgtgg gggttcgact ccagcgcagc ctgtccgcag gcacacttga gggtcaacaa
     2461 aggcggtcgg taccgagggt cggtgcccaa ggagccaaac tgggggcgtg tctctaatta
     2521 gtggctatgg cgtcctcgcc ctcggcttca tgatgatcat gtacgcgctc gagcaacgcg
     2581 gccggctatg catcttggcc tttgctctcg gatgccttct ctcgagcgtc tatggctttc
//
LOCUS       DATCQR010000165         3105 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_210798, whole genome shotgun sequence.
ACCESSION   DATCQR010000165 DATCQR010000000
VERSION     DATCQR010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3105
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210798"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2009)
                     /gene="uvrB"
                     /locus_tag="VIN32_05500"
     CDS             complement(<1..2009)
                     /gene="uvrB"
                     /locus_tag="VIN32_05500"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HEY8922068.1"
                     /translation="MPDLKVHAPFEATGDQPTAIAALVDGVQTGLKHQVLLGATGTGK
                     TFTAAKVIEAIGKPTLVMAHNKTLAAQLYQEFREFFPDNSVEYFVSYYDYFQPEAYLP
                     RSDTYIEKDSSRNDEIDRLRHNATRALFERRDVIIVASVSCIYGLGAPVDYGATVINL
                     KVGGHYRRDGILRHLVDLQYQRNDAAMARARFRVRGDVLEVQPPHGEYLVRVDFFGDE
                     VEKIVEVDSLTGEVLAERKEVNFYPASHYVTPRDKLLVAITKIEAEMEERVAWLSSEG
                     RDLEAARLKQRTDFDLEMMREVGYCSGIENYSRHLAGREQGSRPWTLIDYFPTDFLLI
                     VDESHISVPQVHGMYKNDRTRKEILVDFGFRLPSALDNRPLTFEEWEDHINQVIYMSA
                     TPGPYETERAERVAEQFIRPTGVIDPQITVRPTAGQIDDLLAEIRETVERGERALVTT
                     LTKKMAEDLADYLAELGIKVQWLHSEVDTLERVEILRDLRLGVYDVLVGINLLREGLD
                     LPEVTLVAILDADKEGFLRSGGSLIQVIGRAARNIGGRVIMYADRETDSMRAAIEETT
                     RRRERQVAYNTEHGIVPETIIKEIRDINDGLRRVAEERAELDVAGKEPEELAAMMGRL
                     EAQMKEAARNLEFERAAALRDEILQLRRLREELAAAPGSIPADVYA"
     gene            complement(2055..2627)
                     /gene="coaE"
                     /locus_tag="VIN32_05505"
     CDS             complement(2055..2627)
                     /gene="coaE"
                     /locus_tag="VIN32_05505"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008476513.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEY8922069.1"
                     /translation="MKVIGLTGNIGCGKSTVSGMLRDLGVATIDADEVAREVRNNDGP
                     ARDRIERRFGTSDAGKLAAIVFADPEALADLEAILHPPVRDAVRTRLAELMDAGAEVA
                     CIEAIKLLESPLLDRCDEIWVVRCDEADALGRLAARGLDEEAARARLANQTAQEDKVA
                     AANRVIDGSASIDETRRQVEAAYEALTAVR"
     gene            complement(2624..>3105)
                     /locus_tag="VIN32_05510"
     CDS             complement(2624..>3105)
                     /locus_tag="VIN32_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="zinc finger domain-containing protein"
                     /protein_id="HEY8922070.1"
                     /translation="PYRVGDVFARHGPEPLAPSFSSARLAARLKGRSARLKTLLLDQT
                     FIAGVGNIYADEALWRARLHPLRAADTLTPEEMRRLHRAIRAVLRQGIANRGTSFGDY
                     VGADGEPGDNAERLAVYQRTGQPCLRCGRPISRIVVGQRSTHFCPHCQVLPDEAGPG"
BASE COUNT          438 a         1106 c         1032 g          529 t
ORIGIN      
        1 gcgtagacgt cggcggggat gctccccggg gcggccgcca gctcctcgcg caggcggcgc
       61 agctggagga tctcgtcgcg cagcgcagcg gcacgctcga actccaggtt gcgcgccgcc
      121 tccttcatct gagcctcgag ccggcccatc atcgcggcca gctcctcggg ctccttgcct
      181 gccacgtcga gctcggcacg ctcctcggcg acgcggcgca gcccgtcgtt gatgtcgcgg
      241 atctccttga tgatcgtctc ggggacgatc ccgtgctcgg tgttgtaggc gacctgtcgc
      301 tcccgacggc gcgtcgtctc ctcgatcgcg gcgcgcatcg aatcggtctc tcgatcggca
      361 tacatgatca cccgcccacc gatgtttcga gcggcccggc cgatgacctg gatcagggag
      421 ccgcccgaac gcaggaagcc ctccttgtcg gcgtccagga tggcgaccag cgtcacctcc
      481 ggcaggtcca gcccctcgcg cagcaggttg atgccgacca ggacgtcgta cacccccagc
      541 cgcaggtcgc gcaggatctc gacccgctcg agggtgtcga cctcgctgtg cagccactgc
      601 accttgatcc cgagctcggc caggtagtcc gccaggtcct cggccatctt cttggtgagg
      661 gtcgtgacca gcgcccgctc gccgcgctcc accgtctcgc gaatctcggc cagcaggtcg
      721 tcgatctggc cggcggtcgg ccgcaccgtg atctggggat cgatcacccc ggtcggccgg
      781 atgaactgct ccgcgacccg ctccgcccgc tccgtctcgt agggtccggg agtggcgctc
      841 atgtagatca cctggttgat gtggtcctcc cactcctcga aggtaagcgg ccggttgtcg
      901 agcgccgagg gcaggcggaa tccgaagtcg accaggatct ccttgcgggt ccgatcgttc
      961 ttgtacatgc cgtgcacctg cgggaccgag atgtggctct cgtccacgat cagcaggaag
     1021 tcggtcggga agtagtcgat cagggtccac ggccggctgc cctgctcccg gccggccagg
     1081 tgccggctgt agttctcgat cccggagcag tagccaacct cgcgcatcat ctcgaggtcg
     1141 aagtcggtgc gctgcttgag gcgcgcggcc tccagatcgc gaccctcgct ggagagccag
     1201 gcaacccgtt cctccatctc cgcctcgatc ttggtgatcg ccaccaggag cttgtcgcgc
     1261 ggagttacgt agtgggaggc cgggtagaag ttgacctcct tccgctcggc cagcacctcg
     1321 ccggtcagcg agtcgacctc gacgatcttc tccacctcgt cgccgaagaa gtcgacccgc
     1381 acgaggtact cgccgtgcgg cggctgcacc tccagcacgt cgccacgcac ccggaatcgc
     1441 gcgcgcgcca tggccgcatc gttgcgctgg tactggaggt cgacgaggtg ccgcaggatc
     1501 ccgtcgcgcc gatagtggcc gccgaccttc aggttgatga ccgttgcccc gtagtcgact
     1561 ggtgccccca ggccgtagat gcacgagacg gacgccacga tgatcacgtc gcgccgctcg
     1621 aacagggcgc gcgtggcgtt gtgccgcagc cgatcgatct cgtcgtttcg cgacgagtcc
     1681 ttctcgatat aggtgtccga ccgcggcagg taggcctccg gctggaagta gtcgtagtag
     1741 ctgacgaagt actcgaccga gttgtcgggg aagaactcgc ggaactcctg gtagagctgc
     1801 gcggccagcg tcttgttatg cgccatgacc agcgtcggct tgccgatcgc ctcgatcacc
     1861 ttggccgctg taaaggtctt cccggtcccg gtggcgccca gcaggacctg gtgcttcagc
     1921 ccggtctgca ccccatccac cagcgccgcg atggcggtcg gttgatcgcc ggtcgcctcg
     1981 aatggcgcgt ggaccttgag atcgggcatg gaggatgggc ctcgtggcga cggacggagg
     2041 ggcggggctg aattctagcg aaccgccgtc agggcctcat aggcggcctc cacctggcgt
     2101 cgggtctcat cgatcgaggc cgagccatcg atcacccgat tggccgccgc gaccttgtcc
     2161 tcctgcgcgg tctggttcgc cagtcgcgcc cgagcagcct cctcgtcgag gccgcgcgcg
     2221 gccagccggc ccagcgcgtc ggcctcgtcg cagcggacca cccagatctc gtcgcaccgg
     2281 tccaggagcg gcgactccag cagcttgatg gcctcgatgc aggccacctc cgcaccggcg
     2341 tccatcagct ctgccagccg ggtgcgcacc gcgtcccgca ccggcggatg caggatcgcc
     2401 tcgaggtcgg cgagcgcctc ggggtcggcg aacacgatcg cggccagctt gcccgcgtcc
     2461 gacgtgccga agcgccgctc gatgcggtct cgggcgggcc cgtcgttgtt gcggacttcg
     2521 cgggccacct cgtcggcatc gatggtggcg acccccaggt cgcgcagcat gcccgagacg
     2581 gtgctcttgc cgcagccgat gttgccggtc agcccgatga ccttcatccc ggccccgcct
     2641 catcgggcag cacctggcag tggggacaga agtgcgtgct gcgctgcccc accacgatcc
     2701 gggagatcgg gcgcccgcag cgcaggcagg gctgccccgt tcgttggtag accgccagcc
     2761 gctcggcatt gtcgcccggc tcgccatccg cgccgacgta atcgccgaag ctggtcccgc
     2821 ggtttgcgat cccctgccgg agcacggcgc ggatagcacg atgcagccgt cgcatctcct
     2881 ccggcgtcag cgtgtcggcc gcgcggagcg ggtgcagccg tgctcgccac agcgcctcat
     2941 ccgcgtagat gttcccgacc ccggcgatga aggtctggtc cagcaggagc gtcttcagcc
     3001 gcgcggatcg tcccttgagt cgagccgcca ggcgggccga gctgaaggag ggcgcgagtg
     3061 gctccggccc gtggcgcgcg aacacgtcgc ccacccggta cggct
//
LOCUS       DATCQR010000166        10209 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_101736, whole genome shotgun sequence.
ACCESSION   DATCQR010000166 DATCQR010000000
VERSION     DATCQR010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 10209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10209
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101736"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            225..1148
                     /locus_tag="VIN32_05515"
     CDS             225..1148
                     /locus_tag="VIN32_05515"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEY8922071.1"
                     /translation="MGVDGSDASLTALDLVTATVWPTDTKIRLVGAFELPADWIGRAP
                     SPSDPAADDEAQRALFNTLQRLSEPIRRQGYATETVVARGRAADVLLAEADDVSADLI
                     VVGSRGLGVAASALLGSVSATLVDHAVCPVLVARGPRITRILVATDGSQSAEAIPAVV
                     AAWNVFRDAPINVLSVAPPSTQGEGTTLMRGMLGSDPPQLDAWHEVDRHRVMADEMAA
                     RLVAAGWHAESSVRVGEAAREIETAAADLGADVIITGSRGLGGLQRLLMGSVAHHVLL
                     HSRCSVLVMRGHVPARQQRPLRPAQTAPSAR"
     gene            1449..1844
                     /locus_tag="VIN32_05520"
     CDS             1449..1844
                     /locus_tag="VIN32_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012182569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY8922072.1"
                     /translation="MTLATLKVTMPFSEDILARAKRNTFFREVVSTGPHAQVVVMSIP
                     PEGEIGEEVHYDVDQVLVFVGGEGAAVLEGRRSSVTPGRLVHVPAGTRHNFVNDGSVD
                     LKLYTVYAPPQHAPGTIHRTKAEADDQHA"
     gene            1854..3041
                     /locus_tag="VIN32_05525"
     CDS             1854..3041
                     /locus_tag="VIN32_05525"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY8922073.1"
                     /translation="MNDRSTLIRTAFVSTYPPWRCGIATFTHDLAAVSGWREIVALSP
                     PDHQSPYPAEVHHRIRRDEAGDYARVAHALNHCGIDVVSIQHEYGIWGGADGEHVLEF
                     LHALEIPAVATLHTVLRHPTLRQRQILVELIGATEATVVMSRAAAALLTEAYGIDPGR
                     VDVIPHGVHNLPLVDPETMKPGLGLGGHAVILSFGLLGHGKGYEVAIEAMPAVVAAIP
                     TARYVVLGATHPGLLRHEGETYRQRLVDRVDALGLADQVQFVDRFLGRVELSHWLEAA
                     DVFVTPYPNLEQIVSGTLAYAMGAGKAIVSTPYAYAAELLADGRGILVPPGSSTALGA
                     AFIALLRDANWRAELGARAYSHSRTMIWPEVGNAYRRLFARIAGRPAVPKALAATLAA
                     TSV"
     gene            3034..4131
                     /locus_tag="VIN32_05530"
     CDS             3034..4131
                     /locus_tag="VIN32_05530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8922074.1"
                     /translation="MSDRAPLYPVSRRHLEAISDEIAIIQHALGAQPDLTHGYCTDDV
                     ARALLVDLLHAAEIGWAEVAPSAWRAIRFLESAFADDNGRFRNFRGMAGDWLIGPSSE
                     DAHARAMMALGQAAGVGADDRFRSLAIALFQRGLPGIAQLTYLRPQAAALIGCDAAVR
                     SGLGGETEATYRRLAATLWHRVESGTTPGPWPWPETVLTYENGLIPRALIVGGHRLGY
                     PHMVRRGLDLLDWLVDVETSTTGHVSVVGNAGWWARDGVRARYDQQPIEATALLLAAE
                     AALVVTGDEDHRSTMERCYGWFLGRNDGDVVIALPERGACHDGLTPSGVNRNQGAESS
                     LMWLIALERIRQLRRTSDARTCDAVPVHVPL"
     gene            4188..5114
                     /locus_tag="VIN32_05535"
     CDS             4188..5114
                     /locus_tag="VIN32_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosidase"
                     /protein_id="HEY8922075.1"
                     /translation="MEDLPYPANAIFNPGAAQVGAEALLLVRVEDFRGISHLHVARSV
                     DGVTDWRFDPAPLLVAEPDRYPEEIWGCEDPRLTWLPERDEWAISYTAYSRRGPLVSL
                     ATTTDFRSVQRLGPVMPPEDKDAALFSRRLAGRWAMIHRPVPLHGGAHMWVSYSPDLR
                     HWGDHALLLEAREGAWWDAGKIGLGPPPIETPDGWLVMYHGAHLAASGPIYRVGFALL
                     DLEDPTIVLRRSDEWAFGPREPYEIHGDVHHVVFPCGWIVDELRDEVRLYYGAADTRI
                     GLATARLSAVLDYVRACPVGQQRRREDPSEGN"
     gene            complement(5557..7581)
                     /locus_tag="VIN32_05540"
     CDS             complement(5557..7581)
                     /locus_tag="VIN32_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013284025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8922076.1"
                     /translation="MSGPGGNGRQDSGTPPGQDPAGRPAQSPAGGPGARRGPGMMGGM
                     IPTGAKAQNFRASFRRLLERLRPDAPLIGLVFAFAIVSVLFAILGPKILGNATNDIFQ
                     GVIGKQLPVGATKEQVVAALRAKGQNQFADMVQAMNLTPGVGVDFAGLAQILVLLVGL
                     YLVSSVFSWMQAYVMAGVTQRTVYRLRQDVDRKLGRLPLKYFDSHSRGDLLSRVTNDI
                     DNIGQTLQQSLTQLITSVLTVIGILVLMLTIDPILALLSVLAVPLSIVVTIQIAKRSQ
                     KEFVAQWASTGTLNGRVEEMHTGHAIVKAFGRQEEAIATFEEENNRLYGASYRAQFIS
                     GIIQPAMNFIANLNYVAIAVIGGLRVASGTMTLGDVIAFIQYSRQFTFPIVQTASIAN
                     VLQSAVASAERVFELLDEEEEIPDPVAPQRLRQARGQVAFEQISFRYEPDIPLIEDLS
                     LIADAGETVAIVGPTGAGKTTLVNLLMRFYELDGGRITLDGIDTRDMTRDDLRRTFGM
                     VLQDTWLFHGTIRENIAYGRDAVTDEELIAAAEAAHVDHLVRTLPDGYDTVIDDDASN
                     VSAGEKQLLTIARAFLADPPVLILDEATSSVDTRTEVLIQQAMARLMKGRTTFVIAHR
                     LSTIRDADMILVMNRGRIVEQGTHDELLARRGFYFDLYNSQFVEALAEAS"
     gene            complement(7578..9332)
                     /locus_tag="VIN32_05545"
     CDS             complement(7578..9332)
                     /locus_tag="VIN32_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020576732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8922077.1"
                     /translation="MIRLLRTNLRAYTWPLAAVIVLLLVQAIGNLYLPTLNGDIINFG
                     VAKGDNGYILRLGGFMLLVTLAVGVASIIAVYGGARISMGFGRDVRSAIFRKVETFSQ
                     VEVNRFGPASLITRNTNDVQQVQMVVFMALTLMISAPILIVGGIIMAVRQDVPLSGLL
                     VVVLPIMVAFIGLVMWRAIPLFRAMQTKLDRINQVMRETLSGVRVIRAFVRTEHEEQR
                     FDDSNQDLFATSLRVNRLFAVTIPVMTAILNLSTVAVMWFGAIRVNSGDMPIGNLTAF
                     LQYLMQILFAVLMAVFMFILVPRAAVSAGRIQEVLDTEPMVRDPEHPVAFPAGEPGAE
                     RRGATIEFRNVEFRYPGAEEPVLHDISFTARPGETTAIVGSTGSGKSTLVNLIPRLYD
                     ATEGAVLVDGVDVREVSRTDLWARIGLIPQKAFLFSGTVASNLRYGDEQATDDDLWRA
                     LEIAQSREFVSEMDGALEAPVTQGGTNVSGGQRQRLAIARALVKQAPIFIFDDSFSAL
                     DFATDARLRAALARELGWATVIIVAQRVGTIMKADRILVMDAGRIVAIGTHTDLLENN
                     ETYREIVYSQLTQAEAVA"
     gene            complement(9329..9868)
                     /locus_tag="VIN32_05550"
     CDS             complement(9329..9868)
                     /locus_tag="VIN32_05550"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY8922078.1"
                     /translation="MTRNISEALNNPVVGKRAVSRAMRRAILEQAVIDEMTSWNPLEF
                     LGTFKSMHKGSLSLVHLNVLVELATQGPMPMGRLAEALEVSVASTTGIVDRMEKRGFV
                     ERRHDDEDRRVVVVHPTDAAANFFSSLQQRRRDGVARLLDTLSDRQLLGLVSGHRALR
                     RARARLVAEATEGTAERAP"
     gene            10021..>10209
                     /locus_tag="VIN32_05555"
     CDS             10021..>10209
                     /locus_tag="VIN32_05555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8922079.1"
                     /translation="MKLSRNVMALAVVLVVNVLLVSLLTPLGFESRPATDLKTVGYIA
                     IGTIFTGLALDVASFALLF"
BASE COUNT         1638 a         3374 c         3454 g         1743 t
ORIGIN      
        1 ctgatcctga cgggcgcggg cctggtatgg gccacgcggc ctgagtcgca gccggtccca
       61 gttctgcggc cgatgaacgt gccggcctga ggggaccact cgcgggtccc gtcacccggc
      121 cgtaaccccg ggaagcctga tccaaacggc gagcgaccaa ctcgccgggg tcaggctcct
      181 ggctcttctg ccaagaaaca cgaggtgcag cgatgcggat tgtgatgggg gttgatggtt
      241 ccgatgcctc gctgaccgcg ttggatttgg tgacggcaac ggtctggcca accgatacaa
      301 agatccggct ggtgggcgct ttcgagctgc cggccgattg gattggccgc gcgccatcgc
      361 catcggatcc cgccgcagac gacgaggcgc agcgcgcgct cttcaacacg ctccagcgcc
      421 tttcggaacc gatccgccgg cagggctatg ccaccgagac ggtagtcgct cggggtcgcg
      481 ctgccgatgt tcttctggcc gaagcggacg atgtgagtgc cgacctgatt gttgtcggca
      541 gtcgcgggct cggagtggct gcctccgctc tgcttggctc ggtctccgcg acgctcgtcg
      601 accatgccgt ttgcccggtc ttggtggccc gggggccacg tattactcga atcctcgtcg
      661 ccaccgacgg atcgcagagc gccgaagcca tcccggccgt ggtcgcagca tggaacgtct
      721 tccgcgacgc gccgatcaac gtcctgtccg tcgccccgcc ttcgacccag ggcgaaggga
      781 ctacgctcat gcgggggatg ctcggcagtg atccgccgca gctggatgcc tggcatgagg
      841 tcgaccgtca tcgcgtcatg gcggacgaga tggcagcgcg tttggtggct gccggctggc
      901 atgcggagag ctccgtacga gtcggcgagg ccgctcgaga gatcgagacc gccgcagccg
      961 acctgggtgc ggacgtgatc atcaccgggt cgcgtggact gggcggtctt cagcggctcc
     1021 tcatgggcag cgtggctcac cacgtcctgc tgcattcccg gtgctccgtg ttggtcatgc
     1081 gcggacacgt gcctgcgcgc caacaacgtc cgctccggcc cgctcagacc gcaccgtctg
     1141 cacgctgaag actgctgcac tgccacgctg ctggccggtg taagccacag cgtaagccac
     1201 agcctcgaga cggtcgcggc gagcgacgac gatgggacag acgcagcggc cgcccctcgt
     1261 ccacgatcac gacggctggc gcccgtgctc ggtgccgccg gcggactgag ctcccgggag
     1321 gctaagcctc gcgcgcgggg gcgggggcgg caccctggag ccggcggtcc gttgggccga
     1381 ttacaggacg ttcggctcta gcggggtggg gagccgtctc gcacactcgg gagacaaagg
     1441 agattgccat gacgcttgcc accctgaagg taaccatgcc gttcagcgag gacatcctcg
     1501 cccgagcgaa gcgcaacacg ttcttccgcg aggtcgtctc gaccgggccc cacgcccagg
     1561 tcgtggtgat gagcatcccg cccgagggcg agatcggcga ggaggtccac tacgatgtgg
     1621 accaggtcct cgttttcgtc gggggagaag gcgcggccgt cctcgagggc cgacgcagct
     1681 cggtgacgcc cggtcgcctc gtccacgtcc ccgccggcac gcgccacaac ttcgttaacg
     1741 atggctcggt cgacctcaag ctctacactg tctacgcccc gccccagcac gcgcccggca
     1801 cgattcaccg gaccaaggcc gaggccgacg atcagcacgc ctgagccacc cccgtgaatg
     1861 atcggagtac cctcattcga accgcatttg tctcgaccta tcccccgtgg cgctgcggga
     1921 tcgccacctt cacgcatgac ctggccgcag tctcgggctg gcgtgagatt gtggcgctca
     1981 gcccgccgga ccaccagtcg ccgtacccgg ctgaggtcca ccacaggatc cggcgcgacg
     2041 aggcgggaga ctatgcccgc gttgcccatg cgctcaatca ctgcgggatc gacgtcgtgt
     2101 cgatccaaca tgaatacggg atctggggtg gggccgacgg cgaacacgtg ctggagttcc
     2161 tccacgccct ggagatccct gccgtggcaa cgctgcacac ggtgctgcgt catccaacgc
     2221 tccggcaacg acagatcctc gtcgagctga tcggcgcgac cgaagcgacg gtcgtcatgt
     2281 cgcgcgccgc ggccgcgctg ctgaccgaag cctacgggat cgatccgggc cgggtcgacg
     2341 tgatcccgca tggggtgcac aacctgccgc tggtcgatcc ggaaacgatg aagccgggtc
     2401 ttgggcttgg cggccacgcg gtgatcctga gcttcggcct gctcggccac ggcaagggct
     2461 acgaagtcgc gatagaggcg atgccggcgg tggtggctgc catcccgacc gctcggtatg
     2521 tggttcttgg agcaacccac cccggactcc tccgccatga gggcgaaacg taccgccagc
     2581 gcctggtcga tcgggtcgac gcgctcggcc tggccgacca ggttcagttc gtcgatcggt
     2641 tcctgggtcg ggtggagctg agtcattggc tggaggccgc cgatgtcttc gtaacgccgt
     2701 atcccaatct cgagcagatc gtttccggca cactggcgta cgccatgggc gcgggcaagg
     2761 cgatcgtctc gaccccatat gcctacgccg ccgagctgct ggcggacggt cggggcatcc
     2821 tcgtgccgcc cggatcatcc accgcactcg gcgcggcctt catcgcgctc ttgcgagatg
     2881 cgaactggcg cgccgagttg ggagcgcgag cgtacagcca cagccggacg atgatctggc
     2941 cggaggtggg gaacgcatac cgtcgcctct ttgccaggat cgccggcagg cccgccgtcc
     3001 cgaaagccct cgcggcaact ctggccgcga ccagtgtctg accgagcgcc actctatcca
     3061 gtcagccggc gccatcttga agcgatctcc gacgagatcg cgatcattca gcatgcgctg
     3121 ggcgcccagc ccgatctcac gcacggctac tgcaccgacg atgtggcgcg ggcgctcctg
     3181 gtggatctcc tgcacgctgc ggagatcggc tgggcggagg tcgcgcccag cgcctggcgt
     3241 gccatccgct tcctcgagag tgccttcgcg gacgacaacg gacggtttcg caatttccgg
     3301 ggcatggccg gcgactggct gatcgggccg agctcagagg acgcccatgc gcgggcgatg
     3361 atggcgctcg gacaggcggc cggggtcggg gccgacgatc gtttccggag tcttgcgatc
     3421 gcgctcttcc agcggggcct gcctggcatc gcgcagctga cgtacctgcg cccccaggcc
     3481 gctgccctga tcggctgcga cgcggcggtc cgcagcggtc tcggcgggga gacggaggcc
     3541 acctaccggc ggttggcggc gacgctctgg caccgggtcg agtcggggac gacgcccggt
     3601 ccgtggccgt ggccggagac cgtgctcacg tatgagaacg ggctcatccc ccgtgcgctc
     3661 atcgtcggcg ggcaccggct cgggtacccg cacatggtcc gccgcgggct ggacctcctg
     3721 gactggctcg tggacgtgga gacgtcgacc acgggccacg tgtccgtagt gggcaacgcc
     3781 ggttggtggg ctcgcgacgg ggtgcgtgct cgatacgatc agcaaccgat cgaggccacc
     3841 gccttgctct tggccgcgga agctgccctc gtggtgaccg gcgacgagga ccaccgatcg
     3901 accatggagc gctgctatgg ctggttcctt gggcgcaacg acggcgacgt ggtcatcgcc
     3961 ctcccggagc gaggagcgtg ccacgacggc ctcacgccgt ccggcgtcaa tcgaaaccag
     4021 ggtgccgagt ccagcctgat gtggctgatt gccctcgagc gcatccggca gctgcgccgg
     4081 acgagcgacg cccgcacctg cgatgctgtg cccgtgcacg tgccgctgtg acctctccac
     4141 agaccgatgg gctcttcgaa cgcagcccac gcaatcccat cctcacggtg gaggacctgc
     4201 cctacccggc caacgccatc ttcaaccccg gggccgccca ggtcggagcc gaggcgctcc
     4261 tcctggtccg cgtggaagac ttccggggca tctcgcacct tcacgtggcg cgcagcgtcg
     4321 acggtgtcac cgactggcgc ttcgaccccg caccattgct ggtagccgag cccgatcgtt
     4381 atcccgagga gatctggggc tgcgaggatc cccggctcac ctggctgccc gagcgcgatg
     4441 agtgggccat cagttacacg gcctacagcc gacgggggcc gctggtttcc cttgccacca
     4501 cgaccgattt ccggagcgtc cagcgactcg gcccggtcat gcctcccgag gacaaggacg
     4561 ccgcgttgtt ctcgcggcgg cttgccggtc gatgggccat gatccatcga ccggtgccgc
     4621 tgcacggtgg cgcccacatg tgggtctcct actcgccaga cctccgccac tggggcgacc
     4681 acgcgctcct cctggaggcg cgggaaggcg cctggtggga cgcgggcaag atcggcctgg
     4741 gaccgccacc gatcgaaacc ccggacggct ggctcgtcat gtatcacggc gcccatctcg
     4801 ctgccagcgg accgatctat cgtgtcggct tcgcgctcct ggatctcgag gaccccacga
     4861 tcgtacttcg ccgaagcgac gagtgggcct tcggcccgcg tgagccctac gagatccatg
     4921 gtgacgttca ccatgtcgtg ttcccgtgcg gctggatcgt cgacgaactc cgcgacgagg
     4981 ttcgcctgta ctacggagcc gccgacaccc ggatcggtct ggcgacggcc cggttgagcg
     5041 ccgtgctcga ctacgttcgt gcctgtccgg tgggccagca acggcgccgg gaagacccaa
     5101 gcgagggcaa ctagtccccc tcgactcctt ggggttaagg tcccacgcta agattgagtt
     5161 ccggaggggt ggcagagtgg acgaatgcgg tggtcttgaa actggccgct tttgacgttt
     5221 ctgcctcaga ccgcactctc gccacgctgc agactgctgc actgtcacgc tgggggccgg
     5281 tgtatgccac agcgtaagcc acagtggttc ttgagtgttc gccggcgaac gtcccggagc
     5341 caagcgaagt aatcggcgca ggccggggcg tctatgccta cctgatcgag ggcggggcga
     5401 cgttcaacgg cgaggaggtc ccaccggcga tgccgccaag gtgacggatg agccgcagtt
     5461 cgccatccgc gccgttgaac cgagcgagct gatcctcgtg gacgtgccca tgaccttcga
     5521 gccggtcggg atctggcgcg ggcgcatctg agccgctcag gaagcctcgg cgagcgcctc
     5581 cacgaactgg ctgttgtaga ggtcgaagta gaagccacgc cgggcgagga gctcgtcgtg
     5641 agtgccctgc tcgacgattc ggccccggtt catgaccagg atcatgtccg cgtcgcggat
     5701 ggtcgaaagc cgatgggcga tgacgaacgt ggtgcgcccc ttcatgaggc gcgccatcgc
     5761 ctgctggatg aggacctcgg tgcgggtgtc gacggagctc gtggcctcgt cgaggatcag
     5821 caccggcgga tcggcgagga acgcgcgggc gatggtcagc agttgcttct ccccggccga
     5881 gacgttcgat gcgtcgtcgt cgatgacggt gtcgtatccg tcgggcagcg ttcgcaccag
     5941 gtggtcgacg tgcgccgcct cggcggctgc gatgagctct tcatcggtga ccgcgtcgcg
     6001 gccgtaggcg atgttctcgc ggatcgtgcc gtggaagagc caggtgtcct ggaggaccat
     6061 gccgaacgtc cgccgcaggt cgtcgcgggt catgtcgcgg gtgtcgatgc cgtcgagggt
     6121 gatccggccg ccatccagct cgtagaagcg catcaggagg ttgaccaggg tcgtcttgcc
     6181 ggcgccggtc ggccccacga ttgcaaccgt ctcgcccgca tcggcgatga ggctcaggtc
     6241 ctcgatgagg gggatatcgg gctcgtagcg gaacgagatc tgctcgaagg cgacctggcc
     6301 gcgggcctgt cgaagccttt ggggtgcgac cggatcaggg atctcctcct cctcgtcgag
     6361 cagctcgaag acgcgctcgg ccgaagccac cgccgactgc aggacgttgg caatgctcgc
     6421 ggtctgcacg atcgggaagg tgaattggcg cgagtattgg atgaacgcga tcacgtcgcc
     6481 cagggtcatg gtcccagacg ccacccgcag tccgccgatg accgcaatcg ccacgtaatt
     6541 gaggttggcg atgaagttca tcgcgggctg gatgatcccg gagatgaact gggcccgata
     6601 gctggcgccg tagagccggt tgttctcctc ttcgaaggtg gcgatcgcct cctcctgccg
     6661 cccgaaggcc ttgacgatgg cgtggccggt gtgcatctcc tcgacgcggc cgttcagtgt
     6721 cccggtcgag gcccactggg cgacgaactc cttctgcgag cgcttggcga tctggatggt
     6781 gaccacgatc gagagcggga cggcgagcac cgagaggagg gccaggatcg ggtcgatggt
     6841 cagcatgagc accaggatgc cgatgacggt caggaccgag gtgatcagct gggtgaggct
     6901 ctgctggagg gtctgaccga tgttgtcaat gtcgttcgtc acacggctca gcaggtcgcc
     6961 gcgtgaatgg ctgtcgaagt acttaagggg cagcctgccg agcttgcggt caacgtcctg
     7021 ccgcagccga tagaccgtcc gctgtgtgac gccggccatg acgtaggcct gcatccagga
     7081 gaagaccgag ctgacgagat agagccccac caggaggacg aggatctgcg ccagtcccgc
     7141 gaagtcgaca ccgacacccg gcgtcaggtt catcgcctgg accatgtcgg cgaactggtt
     7201 ctggcccttg gcccgcaggg cggcgaccac ctgttccttc gtggcgccaa ccggcagttg
     7261 cttgccgatc accccctgga agatgtcgtt ggtggcgttg cccaggatct ttggccccag
     7321 gatcgcgaac aggacgctga cgatggcgaa ggcgaagacc aggccgatga gtggcgcatc
     7381 aggccggagg cgttcgagca gccggcggaa cgaggcgcgg aagttttgcg ccttggcgcc
     7441 ggtcgggatc atgccgccca tcattcccgg acctcggcgc gcgccaggtc cgccggcggg
     7501 ggattgggca ggccgcccgg ctgggtcctg gcctggcgga gtgccgctgt cctgccggcc
     7561 attcccgccc ggcccgctca tgccaccgcc tccgcctggg tgagctgcga gtagacgatc
     7621 tcgcgatagg tctcgttgtt ctcgaggagg tcggtgtgcg taccgatcgc gacgatccga
     7681 ccggcgtcca tgaccaggat ccggtccgcc ttcatgatcg ttccgacccg ttgcgcaacg
     7741 atgatcaccg tcgcccagcc gagctcgcga gcgagcgccg cccgcaggcg tgcatcggtc
     7801 gcgaagtcga gcgccgagaa gctgtcgtcg aagatgaaga tcggcgcctg cttcacgagc
     7861 gcccgggcga tcgccagccg ctggcgctgg ccgccggaga cgttcgtgcc accctgggtg
     7921 acgggcgcct cgagcgcgcc gtccatctcg gagacgaact ctcggctctg cgcgatctcc
     7981 agggcgcgcc acaggtcgtc gtccgtcgcc tgctcgtcac cgtagcgcag gttgctggcg
     8041 acggtcccac tgaacaggaa ggccttctga gggatgagcc cgatccttgc ccacaggtcg
     8101 gtgcgactca cctcgcgcac gtcgaccccg tcgaccagga cggccccctc ggtcgcgtcg
     8161 taaaggcgcg ggatgaggtt gacgagcgtc gacttcccgc tcccggtgct gcccacgatc
     8221 gccgtggtct cgcctgggcg agccgtgaac gagatatcgt gcaggactgg ctcctctgcg
     8281 cctgggtagc ggaactcgac gttgcggaac tcgatcgtcg ccccgcggcg ctcggcaccg
     8341 ggctctccgg ccgggaaggc gaccggatgc tccggatcgc gaaccatcgg ctccgtgtcg
     8401 agtacctcct ggatccggcc ggccgagacc gcggcgcgcg gcacgaggat gaacatgaag
     8461 accgccatga gcacagcgaa gaggatctgc atcaggtact ggaggaaggc ggtcaggttg
     8521 ccgatcggca tgtcgccgct gttgaccctg attgcgccga accacatgac ggccaccgtc
     8581 gaaaggttga ggatcgcggt catcacgggg atcgtgaccg cgaacaggcg gttgaccctc
     8641 aatgaggtgg cgaacaggtc ctggttcgag tcatcgaagc gctgctcctc gtgctcggtg
     8701 cgcacgaatg cgcggatgac ccgcacccct gagagcgtct cgcgcatgac ctggttgatc
     8761 ctgtcgagct tggtctgcat ggcccgaaag agcgggatgg cgcgccacat cacgaggccg
     8821 atgaaggcga ccatgatcgg cagcaccacg accagcagcc ctgacagggg aacgtcctgg
     8881 cggacggcca tgatgatccc gccgacgatc aggatcgggg ccgagatcat cagtgtcagc
     8941 gccatgaaga cgaccatctg cacctgctgg acgtcgttcg tgttgcgggt gatgagcgag
     9001 gctggcccga aacggttgac ctcgacctgg ctgaaggtct cgaccttgcg gaagatcgcg
     9061 ctgcgcacgt cgcgcccgaa gcccatcgag atcctcgccc cgccatagac cgcgatgatc
     9121 gatgcgaccc caaccgccag ggtcaccagc agcatgaatc cgccaaggcg caggatgtag
     9181 ccgttgtcac ccttcgccac accgaagttg atgatgtccc cgttgagggt cggcaggtac
     9241 aggttgccga tcgcctggac cagaagcagg acgatcaccg ctgcgagtgg ccaggtgtag
     9301 gcgcgaaggt ttgtgcggag cagccggatc acggggcccg ctccgcggtg ccttccgtgg
     9361 cctcggccac gagtcttgcc cgcgctcgcc gcagcgcgcg gtgaccggag accagtccga
     9421 gcagttgccg gtcgctcagg gtatcgagga gcctcgccac tccgtcccga cgtcgctgct
     9481 ggagggagct gaaaaagttg gcggcggcat cggtcgggtg gacgacgacc acgcgacggt
     9541 cctcgtcgtc gtggcgtcgc tcgacgaacc cacgcttctc catgcggtcg acgatgccgg
     9601 tcgtactcgc gactgagacc tcgagcgctt cggccagtct gcccatcggc atcggcccct
     9661 gggttgcaag ctcgaccagc acgttgaggt ggacgaggct gagcgagccc ttgtgcatgc
     9721 tcttgaaggt gcccaggaac tccaggggat tccaagaagt catctcatcg atgaccgcct
     9781 gctcgaggat cgcgcgtcgc atcgctcgag agaccgcccg cttccccacg accgggttat
     9841 tcagcgcctc gctgatattc cgcgtcacat ggacaatcta cggcgtgcgt gacgattctg
     9901 tcaaaccgtg ccgtgctgcc cccgcgctcg gcactgaacc gtcaccgact gccgcactgt
     9961 cacgctgggg gccgatgtac gccacagccg caaaccacag gatgttacgg tctcccggtg
    10021 atgaaactct ccagaaacgt catggcgctt gccgtcgtgt tggtggtgaa cgtcctgctc
    10081 gtgagcctgt tgactccact cggctttgag agccggccag caaccgactt aaagaccgtt
    10141 ggctacattg cgatcggcac gatttttaca ggtctcgcgc tggacgtagc gtcattcgcc
    10201 cttctcttc
//
LOCUS       DATCQR010000167         8298 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_55545, whole genome shotgun sequence.
ACCESSION   DATCQR010000167 DATCQR010000000
VERSION     DATCQR010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 8298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8298
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55545"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="VIN32_05560"
     CDS             complement(<1..181)
                     /locus_tag="VIN32_05560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8922080.1"
                     /translation="MNGVIAWITLRQLLSRRQTLLLLLLGGVLVLVAGIFRLAGEEAR
                     AVPFTAGLLSNLGIGT"
     gene            complement(178..1104)
                     /locus_tag="VIN32_05565"
     CDS             complement(178..1104)
                     /locus_tag="VIN32_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018216942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8922081.1"
                     /translation="MDEGVVVELTNVSRWYGNVVAVNDISFQLAGGVTGLLGPNGAGK
                     STILHMVAGFLAPSSGSVTVGGEPSWRHPEMYRRIGLVPEREAVYPFLTGREFATANA
                     RLQGMAPAEAAEAAARAIGLVELDDAADRPIGTYSKGMRQRAKVAAALVHDPSILLLD
                     EPFNGMDPRQRLHMMDLLHRMAAQGRTILFSSHILEEVERLAGRVLVIVSGRLAASGD
                     FREIRRLMTDRPHTFTLRSSNDRGLASALLAESTTAGAALDGGILTVRTSDFGAFSRT
                     VTGIARRHQISLHELLPTDESLESVFSYLVRR"
     gene            complement(1116..2024)
                     /locus_tag="VIN32_05570"
     CDS             complement(1116..2024)
                     /locus_tag="VIN32_05570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8922082.1"
                     /translation="MTRREAAVAPRESATGAIYDLGYRAYEGPRLGRRAAFATLFWAS
                     LRAAFGLGRSGRSKLVPWGLTAIMIVPAAVAGAIQALAPGGLSPFNYDNYLWDLQPIL
                     ALFVAAQAPELVSSDQRNHVLSLYFSHALARSDYALAKLAAMAAALFILALAPLLVIL
                     FASILASPDVAGAVGDQLGNLPQIVGSPLIYAVPLAALGMAIASFTPRRAYATGAIIA
                     VFLVSGAVGAILSEAGRGRLSLVAPLINPFVLLDGTRNLLVGKSIPDSPVFRADLDLW
                     VFGVLTAACTLIAIAAVLWRYRRIAA"
     gene            complement(2011..2931)
                     /locus_tag="VIN32_05575"
     CDS             complement(2011..2931)
                     /locus_tag="VIN32_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014137163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8922083.1"
                     /translation="MPLISTSSLTKRYGSSVMGLDGLTLNVEPGIIGLVGANGAGKST
                     LLKILLGLLKPTSGSATVMDMDVATRGPEIRQYVGYLPEHDCLPPDVSATEFVSHMGR
                     MAGLPSAAARERTAEVLRHVGLYEERYRSIGGYSTGMKQRVKLAQAIVHDPKLLLLDE
                     PTNGLDPAGRDEMLDLVHRTGTEFGIATVVASHLLGEIERVCTYLLAIDAGKLLQSAP
                     IASFVERTGTLGVEVEEGADALAQRLVAAGLTAVADGRSVLVDCPDERPYDIVRDVID
                     EMGLSLTRLEQRRHRLEDLFRVEGRTDDPA"
     gene            complement(2955..5402)
                     /locus_tag="VIN32_05580"
     CDS             complement(2955..5402)
                     /locus_tag="VIN32_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013164326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEY8922084.1"
                     /translation="MTSPAATPLTELSLPVTGMTCASCVTRIERFLGRADGVEAAVVN
                     LATERATVRFDPRRIDRGGIVAAIEAAGYEVAPTSAGEADTDAAEHVRETERRALLRD
                     AVLATGIGLAMMVVSLWPGGVPWPMERVNVWMLAPATVVQFVFGRRFLAAAARGLRHG
                     ELTMDTLVSLGTLAAYGYSLAITLIGSADPTYFDSAAVIIGLVLLGRWLEARAKSQAA
                     GAIKALLRLRPTTARVLRDGREADLPIDEVRPGDLLRVRPGERVPADGSIVEGASAVD
                     ESMLTGESVPVDKREGDPVTGATMNASGSFVMRVQRVGTDTTLAQIARLVEQAQGSKA
                     PIQRVVDQVTARFVPAVVLVAAAAFGLWLLLGPEPRLPAALTAAVAVLIIACPCAMGL
                     ATPTAIMVGTAKGAEAGILVRDGAALEHAQRITAIVLDKTGTITRGQPSVVELQPMTG
                     TSESQLLRLAAAAERGSEHPLAEAIVRLAAERSVELPPATDFASISGGGVQATVEGRR
                     TIVGSELLLVEQGVDVQPVEALARETATKGHTPVLVAVDEMAIGVLAIADTVKPESAE
                     AVRRLKAAGLEVWMLTGDRAAVAEAIGAEVGIAPDRILAEVRPGEKAAKIRELQAGGA
                     VVAMVGDGINDAPALAQADLGVAIGTGADVAVEASDITLVGDDLRAVPAAIRLSRATM
                     RTIHQNLAWAFGYNLVLIPVAAGILFPLTGWLLSPALAAGAMALSSVSVVTNSLRLRQ
                     FRPAERTNEPMKIPQAIPQAHDPVCGMSVNPEVATAAGLTAEHEGQSYYFCGRGCKLE
                     FLDDPKRFLDPDYAPHM"
     gene            5495..6718
                     /gene="glgA"
                     /locus_tag="VIN32_05585"
     CDS             5495..6718
                     /gene="glgA"
                     /locus_tag="VIN32_05585"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase"
                     /protein_id="HEY8922085.1"
                     /translation="MKALLLTNEYPPNVYGGAGMHVAELSRQLRKLVSLEIRTFGEQH
                     EEAPGWLVRGYAAPDERRSGDERLRPAWDAFARCLAMAADPSDADLVHCHTWYTHLGG
                     LLVQHANSVPLVVTVHSLEPLRPWKREQLGGGYDLSSWVERSALESADAVIAVSNGTR
                     DDVLSHFAVNPERVHVIHNGIDAEFFAPDPATDALERHGIDPGRPYVLFVGRITRQKG
                     IVHLVRAIRRLDPEVGVVLAAGQPDTAELAAEVEAGVTAAQRERQNVVWIPEMLSREE
                     IRQLYSHAAIFVCPSVYEPFGITNLEAMACERPVVATAVGGIPEVVADGETGLLVPVA
                     FSPDEPMSPADPDQLASDLAAAINALMADPARRTRMGAAGRRRAVEQFSWSSIAAQTA
                     ALYGTLQESASMPPP"
     gene            6715..7479
                     /locus_tag="VIN32_05590"
     CDS             6715..7479
                     /locus_tag="VIN32_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8922086.1"
                     /translation="MTGPIVAAHDLRKRYGSLQAVDGVSFEIAEGEVFGILGPNGAGK
                     TTTLEMIEGMRPIDSGSAVVDGIDVTHDPRGVKARIGIQLQASSFFDELNLVELLELF
                     GHLYERTVDAMALLADVELTEKARSQVKTLSGGQKQRFSIAAALVNDPRVLFLDEPTT
                     GLDPQARHHLWGLVRQIRERGHTVVLTTHYMEEAEELCDRVAIMDRGVIVALDTPKRL
                     VDELIGRGFKKARVERLANLEDVFLDMTGHDLREEA"
     gene            7483..>8298
                     /locus_tag="VIN32_05595"
     CDS             7483..>8298
                     /locus_tag="VIN32_05595"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8922087.1"
                     /translation="MRPFGIRLGMFWALFVASIRMFVRNRAAVFFSLFLPLIIMLIFG
                     VLNFEGTTTIQLGIADEARNQASAKLIDGLAAFDYLEPSSGSRDALLTTLKAGDLDFV
                     LAIPPGFSPQVGQESGLVAYAASGDPQQMQVAQGLLQQAVGQALFAASGGNPTGGGFA
                     APVSFESVASRDLGYIDFLVPGIVGMTVMQLGLFGVAFGFVHLKRTGALRRLFATPTS
                     PAYFLGAQVASRLIIAFVQVVILFGIGLWFGLQMFGDYATLAAIVLLGSIIFLA"
BASE COUNT         1280 a         2905 c         2870 g         1243 t
ORIGIN      
        1 gcgttccgat ccccaggttg ctgagcagcc cggccgtgaa tggcacggcg cgggcctcct
       61 ctcccgccag gcggaagatc cccgcgacca gcaccaggac gccgcccagc aggagcagca
      121 gcagcgtctg tcgccgggag aggagctggc gcagcgtgat ccacgcgatg acgccgttca
      181 cctgcgcacc aggtagctaa agactgactc gagcgattcg tcggtcggca gcagctcgtg
      241 caggctgatc tggtgccgcc gcgcgatccc agtgaccgtc cggctgaatg ccccgaagtc
      301 gctcgtccgc acggtgagga tgcccccgtc gagcgcggcg ccagcggtcg tcgactccgc
      361 cagcagggcg gatgcgaggc cccggtcatt cgaggagcgg agggtgaagg tgtgcggtcg
      421 atcggtcatc aggcggcgaa tctcgcggaa gtcgcccgag gccgccaggc gtccggagac
      481 gatcaccagc acccgcccgg ccaggcgctc gacctcttcg aggatgtgcg acgagaagag
      541 gatcgttcga ccctgcgccg ccatcctgtg cagcagatcc atcatgtgca gccgctggcg
      601 tgggtccatc ccgttgaacg gctcatcgag gagcaggatc gatgggtcgt ggacgagcgc
      661 ggcggccacc ttggcgcgct gccgcatccc cttcgaatag gtgccgatcg gccggtccgc
      721 ggcgtcgtcc agctcgacca ggccgatggc gcgcgcggcc gcctcggccg cctccgccgg
      781 cgccatgccc tgcagccggg cgttggccgt cgcgaactcg cgcccggtca gaaagggata
      841 caccgcctcg cgctccggga cgaggccgat ccggcgatac atctccgggt ggcgccacga
      901 cggctctccg ccgacggtca ccgacccgga cgagggagcc aggaacccgg cgaccatgtg
      961 caggatggtc gacttcccgg ccccgttcgg accgagcagc ccggtgacgc caccagccag
     1021 ctggaagctg atgtcgttga ccgcgaccac gttgccgtac cagcgcgaga cgttcgtcag
     1081 ctcgacgacc acgccttcgt ccattgggct cgtgctcacg cggcgatccg ccgatagcgc
     1141 cacagtaccg cggcgatcgc gatcagggtg caggccgccg tcaggacacc gaagacccac
     1201 aggtcgaggt ccgcgcggaa gaccggggag tccgggatgc tcttcccgac gagcaagttg
     1261 cgagtcccgt ccagcaggac aaacgggttg atgaggggcg ccaccaggga gaggcgtccc
     1321 cggcccgcct cggacaggat cgcccccacc gcaccggaca ccaggaagac ggcgatgatc
     1381 gctccggtcg catatgcccg tcgtggcgtg aaggaggcga tcgccatccc cagcgccgcc
     1441 agcggaaccg cgtagatcaa cggcgaaccg acgatctgcg ggaggttgcc gagctggtcg
     1501 ccgacggcgc cggccacatc gggcgacgcc aagatggacg caaagaggat gaccagcagc
     1561 ggtgcaaggg ccaggatgaa gagcgcggcc gccatcgccg cgagcttggc gagggcgtag
     1621 tcgctgcggg caagcgcatg gctgaagtac agcgacagaa cgtggttgcg ctggtcgctg
     1681 ctgaccagct cgggcgcctg cgcggcaacg aagagggcca ggatcggctg caggtcccag
     1741 aggtagttgt cgtagttgaa gggtgacaac ccgcccggcg cgagggcctg gatcgctccg
     1801 gccaccgcgg cggggacgat catgatcgcg gtcagccccc acggcaccag cttcgagcga
     1861 ccggagcgac cgagcccgaa ggcggcccgc aggctcgccc agaagagcgt cgcgaatgcg
     1921 gccctgcggc ccagccgcgg cccttcgtag gcgcgatagc cgaggtcata gatcgccccg
     1981 gtcgcgctct cgcgcggcgc gaccgccgcc tcacgccggg tcatcggtgc gcccctccac
     2041 gcggaagaga tcctcgagcc gatgccgtcg ctgctcgagc cgggtcaggg agagccccat
     2101 ctcgtcgatc acgtcccgaa cgatgtcgta tggcctctcg tccgggcaat cgaccaggac
     2161 actgcgtcca tcggcgacgg cggtcaggcc agcggccacc agtcgctggg ccagggcatc
     2221 ggccccttct tcgacctcca ccccgagggt cccggtgcgc tcgacgaagc tcgcaatcgg
     2281 agccgactgc agcagcttgc ccgcgtcgat ggccagcagg taggtgcaca cccgctcgat
     2341 ctcgccgagc aggtggctgg cgacgacggt ggcgatcccg aattcggtgc cggtgcggtg
     2401 caccaggtcc agcatctcgt cgcgaccggc agggtcgagg ccgttggtcg gctcgtcgag
     2461 gagcagcagc ttcgggtcgt ggacgatcgc ctgcgccagc ttgacccgct gcttcatgcc
     2521 ggtcgagtag ccgccgatcg agcggtagcg ctcctcgtac agcccgacgt gacggaggac
     2581 ttcggcggtc cgctcgcgcg cagccgcgga cggcagcccc gccatgcgac ccatgtggct
     2641 gacgaactcc gtggcggaga catccggggg caggcagtcg tgctccggaa ggtagccgac
     2701 gtactggcgg atctccggcc cgcgcgtggc cacgtccatg tccatgaccg tcgcgctgcc
     2761 gctggtcggc ttcaggaggc cgagcaggat cttgaggagt gtgctcttgc ctgcgccgtt
     2821 ggcgccgacg aggccgatga tccccggctc gacgttgagg gtcagcccgt ccaggcccat
     2881 caccgaggac ccgtagcgct tcgtcagcga gctggtcgag atgaggggca tcgccccgag
     2941 tctagccgcg cgccctacat gtgcggggcg tagtcagggt cgaggaagcg cttcgggtcg
     3001 tcgaggaact cgagcttgca gccgcggcca cagaagtagt agctctgccc ctcgtgctca
     3061 gccgtcagtc cggcggcggt cgccacctcg gggttcacgc tcatgccgca caccgggtcg
     3121 tgcgcctgcg ggattgcctg ggggatcttc atcggttcat tcgtcctttc agccggtctg
     3181 aactgcctga gccggagcga gttggtcacc acgctgacgc tcgacagcgc catggcaccg
     3241 gcggccaggg cgggggagag aagccagccg gtgaggggga agaggatccc ggcggcaacc
     3301 gggatcagga ccaggttgta tccaaaggcc caggccaggt tctggtgaat ggtgcgcatc
     3361 gtcgcgcgag acaggcggat tgccgcgggg accgcgcgca ggtcgtcgcc tacgagtgtg
     3421 atgtccgagg cctcgaccgc gacgtccgcc cccgtgccga tcgccacgcc gaggtcggcc
     3481 tgcgccagtg caggcgcatc gttgatgccg tcgccgacca tcgcgaccac tgcccctccg
     3541 gcttgcagct ctctgatctt ggccgccttc tcgccggggc ggacctccgc caggatccga
     3601 tccggtgcga tcccgacctc tgcgccgatc gcctcggcca ccgccgcgcg atcgcccgtc
     3661 agcatccaca cctcgagtcc ggcggccttc aggcggcgca ccgcctctgc cgattccggc
     3721 ttgaccgtgt ccgcgatggc gagcacgccg atcgccattt cgtcgaccgc caccagcacc
     3781 ggggtgtggc ccttcgtcgc cgtttcgcgc gccagtgcct cgaccggctg gacgtccacg
     3841 ccctgctcga caaggagcag ctcgctgccg acgatcgtcc gccggccctc gaccgtcgcc
     3901 tgcacgccgc cgccggagat cgaggcgaag tccgttgcgg gcggcagttc gacgcttcgc
     3961 tcggctgcca agcggacgat cgcctcggcg aggggatgct cgctcccgcg ctccgcagcc
     4021 gcggccaggc ggagcagctg actctcgctc gtgcccgtca tcggttggag ctcgacaacc
     4081 gagggctgtc cgcgagtgat cgtgccggtc ttgtcgagga cgatggccgt gatccgttgc
     4141 gcgtgctcca gcgcggcgcc gtcgcgcacc aggatcccgg cctcggcgcc cttggccgtc
     4201 ccgaccatga tggcggtggg cgtggccagg cccatcgcgc aaggacaggc gatgatcagg
     4261 acggccaccg ccgcggtcaa cgccgccggc aaccgcggct cggggccgag cagcagccag
     4321 aggccgaatg ccgcagccgc caccagcacg accgccggca cgaagcgtgc cgtgacctgg
     4381 tcgaccaccc gctggatcgg cgcctttgag ccctgggcct gctcgaccag gcgagcgatc
     4441 tgcgcgagcg tggtgtcggt ccccacccgc tgcacccgca tcacgaacga gcccgacgcg
     4501 ttcatagtcg cgcccgtcac cgggtcgccc tcgcgcttgt cgaccgggac cgactccccg
     4561 gtgagcattg actcgtcgac cgccgacgca ccctcgacga tcgagccgtc ggccggcacg
     4621 cgctctcccg gtcggacgcg cagaaggtca cccgggcgca cctcgtcgat cggcagatcc
     4681 gcttcgcgcc cgtcccgcag cacccgtgcg gtggtcggcc gcagcctgag aagggccttg
     4741 atggcgccgg cggcctggct cttggcacgt gcctcgagcc atcggcccag cagtacgagg
     4801 ccgatgatca ccgcggcgct gtcgaagtag gtcgggtccg cggagccgat gagcgtgatc
     4861 gcaagcgaat agccgtaggc cgccagggtc ccgagcgaga cgagggtgtc catcgtcagc
     4921 tcgccgtggc gcaagcccct tgccgcggcg gccaggaagc gacggccgaa gacgaactgc
     4981 acgacagtgg ccggtgcgag catccagaca ttgacccgtt ccatcggcca cggaacgccg
     5041 cccggccaga gggagacgac catcatcgcc aggccgatgc ccgtcgcgag cacggcgtca
     5101 cgcagcagcg cccgacgctc ggtctcgcgc acatgctcgg ccgcgtcggt atccgcctcg
     5161 cccgcggacg tcggggccac ctcgtagccg gccgcctcga tggcggccac gatcccgccg
     5221 cggtcgatgc ggcgcggatc gaatcgcacg gtggcgcgct cggtggccag gttgacaacc
     5281 gcggcctcga cgccgtcggc gcgccccagg aagcgctcga tgcgggtcac gcacgaggcg
     5341 caggtcatgc cggtgacggg gagcgacagc tcggtgaggg gtgtggccgc ggggcttgtc
     5401 atcggctctt caggatacta caggggagta tctcgaccgt cggttggagg agaccgcggt
     5461 acgggtgacg caacccacgc tacgctgcca ccgcatgaag gcgctgctgc tgaccaacga
     5521 gtacccgccc aacgtctatg gcggcgccgg aatgcacgtg gccgagctgt cccgccagct
     5581 gcgaaagctg gtctcgctgg agatccggac cttcggcgag cagcacgagg aggcgcccgg
     5641 ctggctggtc cgaggctacg cggcgccaga cgagcggcga tcaggcgacg aacgcctccg
     5701 cccggcctgg gatgccttcg cacgctgcct ggccatggcc gccgacccca gtgacgccga
     5761 cctcgtccac tgccacacct ggtacaccca cctcggcggc ctgctggtgc agcacgccaa
     5821 ctcggtcccg ctggtcgtca cggtccactc gctcgagccg ctgcgaccct ggaagcgcga
     5881 gcagctgggt ggcggctacg acctctcgag ctgggtggag cggagcgcgc tcgagtcggc
     5941 cgacgccgta atcgccgtta gcaacggaac gcgcgatgac gtcctcagcc acttcgcggt
     6001 aaatccggag cgcgtgcacg tcatccacaa cgggatcgat gccgagttct ttgcgcccga
     6061 tcccgccacc gatgccctgg agcgccacgg catcgacccg ggccggccgt acgtgctctt
     6121 cgtgggacgc atcacgcgcc agaaggggat cgtgcatctc gtgcgcgcaa ttcgccggct
     6181 cgatcccgag gtcggcgtcg tgctggcagc cgggcagccg gacaccgcgg agctcgctgc
     6241 cgaggtcgag gcaggcgtca ccgccgccca gcgcgagcgg cagaacgtgg tctggatccc
     6301 cgagatgctc agccgcgagg agatccggca gctctacagc cacgcggcga tcttcgtgtg
     6361 cccatcggtc tatgagccgt tcgggatcac caacctggag gcgatggcct gcgagcgtcc
     6421 ggtcgtggcg accgcagtgg gcggcatccc ggaggtcgtc gccgacggtg agacggggct
     6481 gctcgtgccg gtggcgttca gccctgatga gccgatgagt cctgctgacc cggatcagct
     6541 tgccagcgac ctggccgctg ccatcaacgc cctcatggcc gacccggccc gtcggactcg
     6601 gatgggagcc gccggtcgac gccgggctgt cgagcagttc agctggtcgt ctatcgcggc
     6661 gcagaccgcc gccctctacg gcacgctgca ggagtcggct agcatgccgc ctccatgaca
     6721 ggaccgatcg tggctgcgca cgaccttcgg aagcgttacg gcagcctgca ggcggtcgac
     6781 ggcgtgagct tcgagatcgc cgagggcgag gtgttcggca tcctcggccc taacggcgca
     6841 ggcaagacca cgacgctcga gatgatcgag gggatgcggc cgatcgattc ggggagcgcc
     6901 gtcgtcgacg gaatcgacgt cacgcacgac ccgcgcgggg tgaaggcgcg gatcgggatt
     6961 cagctccagg cgtcgagctt cttcgacgag ctgaaccttg tcgagctgct cgagctcttc
     7021 ggccacctct acgagcgcac ggtcgatgca atggcgctcc tcgccgacgt ggagctcacc
     7081 gagaaggcgc gctcacaggt gaagaccctc tccggcgggc agaagcagcg attcagcatc
     7141 gccgccgccc tcgtcaacga cccgcgcgtc ctcttcctcg acgagccgac gaccgggctc
     7201 gacccgcagg cccgacatca cctgtggggc ctcgtgcgcc agattcgcga gcgcgggcac
     7261 acggtggtgc tgaccacgca ctacatggag gaggcggagg agctgtgcga ccgggtcgcg
     7321 atcatggatc gcggcgtgat cgtcgcgctc gacacgccta agcggctcgt cgacgagctc
     7381 atcggtcggg gattcaagaa ggcacgcgtc gagcggctcg ccaacctcga ggacgtcttc
     7441 ctcgacatga ccggtcacga tctccgggag gaggcctgac cgatgcgccc cttcgggatc
     7501 cgcctcggga tgttctgggc gctctttgtc gcgagcatcc ggatgttcgt gcgcaaccgt
     7561 gccgccgtct tcttcagcct cttcctgccg ctcatcatca tgctgatctt cggggtcctg
     7621 aacttcgagg gcaccaccac catccagctc ggcatcgccg atgaggcgcg gaatcaggcc
     7681 agcgcgaagc tgatcgacgg cctggcggcg ttcgactacc tcgagcccag cagcggctct
     7741 cgcgacgcgc tgctcacgac gctcaaggcg ggcgacctcg acttcgtgct cgccatcccg
     7801 ccagggttct cgccgcaggt cggccaggag agcggactgg tggcatacgc ggccagtggc
     7861 gatccgcagc agatgcaggt cgcacagggg ctgctgcagc aggcggtcgg acaggccctc
     7921 ttcgcggcga gcggggggaa tccgaccggc ggcgggttcg cggcgcccgt cagcttcgag
     7981 tccgtcgcgt cgcgcgacct gggctacatc gatttcctgg ttcccgggat cgtgggcatg
     8041 acggtgatgc agctcggcct gttcggtgtc gcgtttgggt tcgtacacct gaagcggacc
     8101 ggcgccctgc gccgtctctt cgcgactccc acctcgccgg cctacttcct gggagcgcag
     8161 gtggcgtcgc gcctgatcat cgccttcgtg caggtcgtga tcctgtttgg gatcggcctg
     8221 tggtttggcc tgcagatgtt cggcgactac gcgacgctgg ccgcgatcgt gctcctgggc
     8281 tcgatcatct tcctggcc
//
LOCUS       DATCQR010000168         7456 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_87100, whole genome shotgun sequence.
ACCESSION   DATCQR010000168 DATCQR010000000
VERSION     DATCQR010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 7456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7456
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87100"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(65..211)
                     /locus_tag="VIN32_05600"
     CDS             complement(65..211)
                     /locus_tag="VIN32_05600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8922088.1"
                     /translation="MAGDAPPPRRSPRDYRPDLRPMLILGGLLLAVILGWILVSPLLL
                     PSAR"
     gene            complement(220..939)
                     /locus_tag="VIN32_05605"
     CDS             complement(220..939)
                     /locus_tag="VIN32_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017716442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8922089.1"
                     /translation="MTGTAHALELVDIHTYYGNIHALKGISLTVDPGEVVTLIGSNGA
                     GKSTTLRTISGIARPREGKVIFNGTEIQAEKAHNIVAKGIAQSPEGRHVFAQMTVYEN
                     LLMGAFSRSGKFDADIDRVYDLFPRLKERRTQKAGTLSGGEQQMLAIGRALMAKPSVL
                     LLDEPSMGLSPILTEQIFKIITDINAQGTTVLLVEQNAQMALNVASRGYVLQTGQIIL
                     ADTAANLAANVQVRQAYLGEI"
     gene            complement(936..1757)
                     /locus_tag="VIN32_05610"
     CDS             complement(936..1757)
                     /locus_tag="VIN32_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8922090.1"
                     /translation="MTAADPEVTTDASADERPLLVATDVTKTFEGLVANRDIDFEIPR
                     HSIVAIIGPNGAGKTTFFNQLSGVYHPTSGTIEFEGIDISGMPPHAVAGLGVARTYQN
                     IRLFQNMTVLANVMVGRHVRMHGQWYQAIAGLGGIRQEEEATRRRAFELLELVGISRR
                     SAADLSKNLPYGDQRRLEIARALASDPKLLLLDEPTAGMNPGETRQMTDFIGRLRREL
                     KLTILLIEHDMKVVMGVSERITVLDYGEKIAEGTPAEIRANPRVIEAYLGKQATA"
     gene            complement(1761..3491)
                     /locus_tag="VIN32_05615"
     CDS             complement(1761..3491)
                     /locus_tag="VIN32_05615"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine/isoleucine/valine transporter permease
                     subunit"
                     /protein_id="HEY8922091.1"
                     /translation="MSLGASLRSAVKVGLITGAVMVYLVAVGIASAFAKREVVTGFLT
                     LGHLMLVIPPLAIGYIVAGRPLPTSQRLTHALVAGLATGVLFAAAFLLAVALSPGIRL
                     VLNRVSSSLLDFVGFRSLGLEPGTGALLNLGLAAALALVGGLVQVAPVTVRRAVVGAG
                     TALVVMSMVEPFLRPRLLQFGLPGAAQFVYRSHGLGMVSAALILALGAVLAVGWPRMR
                     STVRSRIAAADPATRGMLRWAAILVGIAFLYTLPSISGSFLTQVLVLVGFYVLLGLGL
                     NIVVGYAGLLDLGYVAFYAVGAYFTALITSPTSALGWGLNWWIALPIVIVLSAMVGLF
                     IGAPVLRLRGDYLAIVTLGFGEIARVLLGSDALKPWLGGPNGILSVPFMIDDGTYFLP
                     IVGKVTGPAATYFPLMGFCILAAFIAYRLKYSRTGRAWNAMREDEDVAQATGISVVNY
                     KLLAFAMGASVGCVGGAIYATHLQGVVPGGFVLLVSITVLAVVVLGGMGSIPGVIVGA
                     FALIGTPELLREFAEYKLLVYGAVLVAVMVLRPEGLIPDASRRAELEEARQPEEGELQ
                     VEYDEISGEA"
     gene            complement(3492..4538)
                     /locus_tag="VIN32_05620"
     CDS             complement(3492..4538)
                     /locus_tag="VIN32_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020388729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY8922092.1"
                     /translation="MRRPLNLSLVDVILWALRVAIVVGVAVGTITTLSTTRLSGDQWR
                     DLLIFGISQGSVYALIALGYTLVYGVLLMINFAHGDVFMFGGMTAFFAADWLSDNGVL
                     NTAPFIAVPIILLVGAVSSTVVALLLERIAYRPLRRAPRLVPLITAIGASLFISNSVR
                     GLYGDGVMAYPRVDAFQGTFNLLGIPILKTHAIVIVSAVVLMTVLYWFVERTRTGRAM
                     RAVSEDKDTAALMGIDVDRIISITFAIGGALAGAAGVLYAIVYNQVIWSMGFLPGIKA
                     FTAAVLGGIGSIVGAMLGGLVLGVLESLAPTLLLAGFGVPSPNQLRDVIAFSVLVLVL
                     IFRPYGLFGRPESK"
     gene            complement(4669..5979)
                     /locus_tag="VIN32_05625"
     CDS             complement(4669..5979)
                     /locus_tag="VIN32_05625"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HEY8922093.1"
                     /translation="MQKFLRWSAVSTAMLIVLAACTPAGSGSPSGSQAAASASLAADP
                     AAACDALPNGCFEVAAGEPLTIASALSITGDTAFLGNDSNFGIQVAQDERGKVLNRDV
                     EVIKEDAGCGDAATGQTAAQSILGNPAILGVIGTTCSRTAVTAMQVLGPAHVVMVSPS
                     NTAPGLTNPDHEQYGGEFFFRTAYNDKVQGAAVAKYACEVLKVDTAATIHDGSPYAEQ
                     LQQVFADQFKSQCSGTVTSQNAIGVGDVEFHSVLTKIAAETPKLIFMPIFDPEGPLIV
                     NQSRDLPALKDTIFFGADGVKDAGFVKAAGKVAQEIGMYFSGPDLNFGTRYTDDFLPK
                     LLALEGTKQPQAPYHAHGYDAYNILMDAIESAHVGDGADGTSFFSRDGIREHFAGLKD
                     YAGLTGTLTCGAAGHDAGDCGSSEVSIAQLVDGVYTEVYTTRAK"
     gene            6171..6641
                     /locus_tag="VIN32_05630"
     CDS             6171..6641
                     /locus_tag="VIN32_05630"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8922094.1"
                     /translation="MEYHVRSARLTDVDAAIRILMRDPADPDQRRQDTDRLRTLLFMP
                     SATVVVAEAERQVIGIGVLSIRPSVHSGPFIGVIDELGVATATRRSGSAAPAADAAQL
                     KGLSDSILEHLVTSARNKGCTRVDVTDPLATAEPALLKRAGFARRGALLSRATG"
     gene            6719..>7456
                     /locus_tag="VIN32_05635"
     CDS             6719..>7456
                     /locus_tag="VIN32_05635"
                     /inference="COORDINATES: protein motif:HMM:NF014045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEY8922095.1"
                     /translation="MSKNVAVFVDVANLYYSARGLEVDVDYVALLKAATKGRDLIRAY
                     AYSGLDPENENQRKFIDFLGKNDYKVVHKDIRKFGDGRVKANLDIELVVDLFRLADRM
                     DIAVIVSGDGDFAPAIRALQDKGVRCEVISFRPNTSSDLIAVADEFIDIMKIIGIGRG
                     KDADKASKVMSAPPMPAKEVVPEFGFREAPAAVAAAALAAIREGGAKPASRGRGRGGA
                     RPEPAIEAMAPGTEEPPEPPTAAEAAAD"
BASE COUNT         1312 a         2428 c         2528 g         1188 t
ORIGIN      
        1 ccgcggtcgg catcggcctg gtcctgtatg gggcgggccg cggattcggc gcacggcgcc
       61 cggcctaacg agcgctcggc aggagcagtg ggctcaccag gatccagccc aggatgaccg
      121 ccagcagcag gccgcccagg atcagcatcg gccgcaggtc gggccggtag tcgcgcggcg
      181 agcggcgcgg tggaggcgcg tcgccggcca tcggctcggc tagatctcgc ccagataggc
      241 ctgccgcacc tggacgttgg ccgccaggtt ggcggcggta tcggccagga tgatctggcc
      301 cgtctgcagg acatagccgc ggctggcaac gttgagggcc atctgcgcgt tctgctcgac
      361 cagcagcacc gtcgtgccct gggcgttgat gtcggtgatg atcttgaaga tctgctcggt
      421 gaggatgggg gagagaccca tcgagggctc gtcgagcagg aggacgctcg gcttggccat
      481 cagggcccgc ccgatggcga gcatctgctg ctcaccgccc gacagggtcc ctgccttctg
      541 cgttcgccgc tccttgaggc gtgggaagag gtcgtagacc cgatcgatgt cggcgtcgaa
      601 cttgccgctg cggctgaacg cccccatcag caggttctcg tagacggtca tctgggcgaa
      661 gacgtgacgc ccctcaggcg actgggcgat ccctttcgcg acgatgttgt gcgccttctc
      721 ggcctggatc tcggtgccgt tgaagatgac cttcccctcg cgcggccggg cgatgccact
      781 gatggtgcgc agcgtcgtcg acttgccggc cccgttggag ccgatgaggg tcaccacctc
      841 gccggggtcg acggtgagcg agatcccctt cagggcgtgg atgttgccgt agtaggtgtg
      901 gatgtcgacc agctcgagag cgtgcgcggt tccggtcatg ccgtcgcctg cttcccgagg
      961 taggcctcga tgacgcgcgg gttggcgcga atctcggccg gcgtcccctc ggcgatcttc
     1021 tcgccatagt cgagcaccgt gatccgctcg ctgaccccca tcacgacctt catgtcgtgc
     1081 tcgatcagca ggatcgtgag cttgagctcg cgacgcaggc gcccgatgaa gtccgtcatc
     1141 tggcgggtct cgccggggtt catgcccgcg gtcggctcgt cgaggagcag cagctttggg
     1201 tcgctggcca gcgctcgtgc gatctcgagg cggcgctggt cgccgtacgg caggttcttg
     1261 gagagatccg cagccgagcg gcgcgagatc cccacgagct ccaagagctc gaaggcgcgg
     1321 cgcctggtcg cctcctcttc ctggcggatc ccgccgagac cggcgatcgc ctgataccac
     1381 tgcccgtgca tgcgcacgtg gcgacccacc atcacattgg cgagcaccgt catgttctgg
     1441 aagagccgga tgttctgata ggtacgcgcc acgccaaggc cggcgacagc gtggggtggc
     1501 atacccgaga tgtcgatccc ctcgaattca atggtcccgg aggtggggtg atagacgccg
     1561 ctgagctggt tgaagaaggt cgtctttccg gcgccgttcg ggccgatgat cgccacgatc
     1621 gagtgccgcg ggatctcgaa gtcgatgtcg cggttggcca ccaggccttc gaatgtcttg
     1681 gtcacgtcgg tcgcgaccag cagcgggcgc tcgtcggcgc tggcgtcggt ggtcacttcc
     1741 gggtcggcgg cggtcatcgg tcaggcctcc ccgctgatct cgtcgtattc aacctggagc
     1801 tcgccttcct cgggttggcg cgcctcctcg agttcggccc ggcgggacgc atccggaatc
     1861 agcccctcgg gcctcagcac catcaccgcc accagcacgg ctccatagac gagcagcttg
     1921 tactcggcga actcgcgcag cagctccggc gttccgataa gcgcgaacgc gccgacgatg
     1981 acgccgggaa tgctgcccat ccctcccagc accaccaccg ccagcacggt gatcgagacg
     2041 agcagcacga acccgccggg caccaccccc tgcagatgag tggcgtagat cgctccgccg
     2101 acgcagccga ccgaggcacc catcgcgaac gccagcagct tgtagttcac cacgctgatg
     2161 ccggtcgcct gggccacgtc ctcgtcctcg cgcatcgcgt tccaggcgcg gccggtacgc
     2221 gagtacttga gccgataggc gatgaaggcg gccaggatgc agaagcccat cagcgggaag
     2281 taggtcgccg ccggccccgt gacctttccg acgatcggca ggaagtaggt tccgtcgtcg
     2341 atcatgaacg gcacgctgag gatgccgttg ggtccgccca gccatggctt gagtgcgtcc
     2401 gagccgagca ggacgcgcgc gatctcgccg aagccgagtg tgacgatggc gaggtagtcg
     2461 ccgcgcagcc gcagcacagg cgcgccgata aagaggccaa ccatggcgga caggacgatc
     2521 acgatcggca gcgcgatcca ccagttgagc ccccagccga gcgccgaggt cggtgaggtg
     2581 atgagcgcgg tgaagtacgc gccgacggca tagaaggcga cgtagccgag atccaggagt
     2641 cccgcgtagc cgaccacgat attcagaccc aggcccagga ggacgtagaa cccgacgagc
     2701 acgaggacct gggtaaggaa ggacccggag atcgacggga gggtatacag gaacgcgatt
     2761 ccgaccagga tcgcagccca gcgaagcatg ccccgcgttg cgggatcggc ggcggctatt
     2821 cggctgcgca ccgtgctgcg catccgaggc cagcccaccg ccaggaccgc gccgagcgcg
     2881 aggatcagcg ctgcactgac cattcccagc ccgtgcgacc gatagacgaa ttgcgccgcg
     2941 ccgggcaggc cgaattgcag gagacgaggc cgcaggaagg gctcgaccat gctcatcacc
     3001 acgagtgcgg tgccggcgcc cacgactgcg cgccgaacgg tcacgggtgc cacttgaacg
     3061 aggccaccca ccagcgcgag cgcggccgcc agccccaggt tgagaagcgc gccggtgcct
     3121 ggttcaagcc ccaggctcct gaagccgacg aagtcgagga gactgctcga gacccgattc
     3181 aggaccaacc ggatcccggg ggagagggcg accgccagca ggaatgccgc cgcgaagagg
     3241 accccggtgg cgagcccagc cacaagggcg tgagtcagcc gctgggaggt cggcaacggt
     3301 cgaccggcga cgatgtagcc gatggccaga ggcggaatga cgagcatcag gtgacccagg
     3361 gtgaggaagc cagtcaccac ctcacgcttt gcgaaggcgg atgcaatgcc gacggccacc
     3421 aggtaaacca tcaccgcacc ggtgatgagg cccaccttga ccgcgcttcg gaggctggcg
     3481 cccaaggaca tctacttgct ctccggccgg ccgaataggc cataggggcg gaagatcagg
     3541 acgagcacca gcaccgagaa ggcgatcaca tcccgcagct ggttcggcga cgggacgccg
     3601 aagcccgcaa gcagcagcgt tggagccagc gattccagca cgccgagtac gagcccgccc
     3661 agcatcgcac cgacgatgct tccgatgccg cccaggactg cggcagtgaa ggccttgatc
     3721 cccggcagga aacccatcga ccagatgacc tggttgtaga cgatcgcata caggactccg
     3781 gcggctccgg ccagggctcc gccgattgcg aaggtgatgg agatgatccg gtccacgtcg
     3841 atccccatca gcgcagccgt gtccttgtcc tcactgactg cccgcatcgc tcgcccggtc
     3901 cgggtcctct ccacgaacca gtacaggacg gtcatcagca ccacggccga gacgatcacg
     3961 atggcgtgcg tcttcaggat cgggatgccc aggaggttga acgttccctg gaatgcgtcg
     4021 acacgcgggt aggccatgac gccgtcgccg tacaggccgc gcacgctgtt gctgatgaac
     4081 agtgacgcac cgatggcggt gatcagtggc acgaggcgag gggcccggcg caacggtcgg
     4141 tacgcgatcc gctccagcag gagggcgacg accgtggagc tcacggcccc caccagcagg
     4201 atgatcggca ccgcgatgaa gggcgcggtg ttgaggacgc cgttgtcgct cagccagtcg
     4261 gccgcgaaga aggccgtcat gccgccgaac atgaacacgt cgccgtgggc gaagttgatc
     4321 atcagcagca cgccgtagac gagggtgtag ccgagggcga tgagcgcata gacgctgccc
     4381 tgcgagatac cgaagatcag caggtcgcgc cactggtcgc cgctcagacg ggtggtcgac
     4441 agggtcgtga tcgtcccgac cgcgacgccg accacgatcg ccaccctcag cgcccagagg
     4501 atgacgtcta ccagggacag gttgagcggc cgtcgcaccg gacccgggtc ctcctgggtg
     4561 gctgccaatc agacgaggtt tggggagtgt acctagtggg tgcaagagag cgggtgggcc
     4621 ggttaccggc ccacccgctc tgggtgctgg ggtcagatgg tcaggtgctt acttggcccg
     4681 ggtcgtgtag acctcggtgt agacgccgtc caccagctga gcgatcgaga cctcactgga
     4741 gccgcagtcg ccggcatcgt gacctgcggc gccgcaggtc agcgttccgg tgagcccggc
     4801 gtagtccttc aggcctgcga agtgctcgcg gattccgtcc ctcgagaaga aggatgtccc
     4861 gtctgcgccg tcgcccacgt gggccgactc gatcgcatcc atcaggatgt tgtacgcgtc
     4921 atacccatgc gcgtggtacg gggcctgagg ttgtttggtt ccctcgagcg caagaagctt
     4981 gggcaggaag tcgtcggtgt agcgggtacc gaagttgagg tcgggaccgg agaagtacat
     5041 cccgatctcc tgcgcaactt tcccggccgc cttgacgaag ccagcgtcct tgacgccatc
     5101 ggcgccgaag aagatggtgt ccttcagggc ggggaggtcc cgcgactggt tgacaatcag
     5161 cggcccctcg gggtcgaaga tcggcatgaa gatcaacttg ggggtctcag ccgcgatctt
     5221 ggtcagcacg ctgtggaact ccacgtcgcc gacgccaatc gcgttctgag aggtaacggt
     5281 tccactgcac tgagacttga actgatcagc aaagacctgc tgcagctgct ccgcgtacgg
     5341 gctcccgtcg tggatggtcg cggcagtatc gaccttgagg acctcgcagg cgtactttgc
     5401 gactgcggct ccctggacct tgtcgttgta ggcggtacgg aagaagaact caccgccgta
     5461 ctgctcgtga tcggggttgg tcaggcctgg cgcggtgttc gatggcgaga ccattacaac
     5521 gtgggccggg ccaagcacct gcatcgctgt gaccgcggtg cgcgagcacg tggtgccgat
     5581 gactccaagg atcgccgggt tgccgaggat cgactgagcg gccgtctggc cggtggccgc
     5641 gtcgccgcag ccagcgtcct ccttgatgac ctcgacgtca cgattgagca ccttgccacg
     5701 ctcgtcctgc gcgacctgga tgccgaagtt cgagtcattc ccgaggaagg cggtgtcacc
     5761 cgtaatggaa agcgcgctgg cgatcgtcag cggctcgccc gcagcgacct cgaagcagcc
     5821 gttaggcagc gcgtcgcacg ctgcggccgg gtctgctgcc agagaagcgc tcgccgcagc
     5881 ctggcttccg ctcggtgacc cgctcccagc gggcgtacac gcggcgagga cgatgagcat
     5941 ggccgtcgag acggcgctcc aacgcaagaa tttctgcatc cagtgatcct ccaatggctc
     6001 gggtgggcgc gagctcgcca gctcagtaaa gagtggggcg catcataagg gcggccttcg
     6061 ccgcagcgca agaggtctgc gagccttcgc tgagctcacg gggcggcgtg gtatgcttct
     6121 ttgggtacga ctgcagccaa cgcgctgcgt ttcgctcccg agaggctccc gtggagtatc
     6181 acgtccgcag cgcccgtctg accgatgtcg acgccgcgat ccgcatcctt atgcgcgacc
     6241 cggccgatcc cgaccagcgg cgccaggaca ccgatcggct acgcaccctg cttttcatgc
     6301 cgtcggccac cgtggtcgtc gccgaggcag agcgccaggt gatcggcatc ggagtcctct
     6361 cgatccgtcc ctcggtccac tcgggtccgt tcatcggcgt catcgatgag ctcggagtgg
     6421 caaccgcaac gcgacggtcg gggagcgccg cgccggcagc agacgccgcg cagctcaagg
     6481 ggttgagtga ctcgattctc gagcacctgg tcacctcggc gcgcaataag ggttgtacgc
     6541 gcgtggatgt caccgatcct ctcgccaccg ccgagccggc gctgctgaag cgcgccgggt
     6601 tcgcgagacg aggggcgctg ctgagccgcg ccaccggctg aacgtttcag acgtggcgcg
     6661 ccgggccggg accccgcagc gtcaaccaca tcggaatgga aacctaggag aagctcactt
     6721 gagcaagaac gtggccgtct tcgttgacgt tgccaacctt tactactcgg cccgcgggct
     6781 ggaggtcgac gtcgattatg tcgcccttct caaagcggcc accaaggggc gcgacctgat
     6841 tcgcgcctat gcctattccg ggctcgaccc ggagaatgag aaccagcgca agttcatcga
     6901 cttcctgggc aagaacgact acaaggtcgt tcacaaggac atccgcaagt tcggcgacgg
     6961 ccgtgtcaag gccaacctgg acatcgagct ggtggtggac ctcttccgcc tcgccgatcg
     7021 gatggacatc gcggtcatcg tctcgggtga cggcgacttc gctcccgcca tccgcgcgct
     7081 ccaggacaag ggggtccgct gcgaggtgat cagcttcagg ccgaacacga gctcggacct
     7141 gatcgctgtc gccgacgagt tcatcgacat catgaagatc atcggcatcg gccgcggcaa
     7201 ggatgccgac aaggccagca aggtgatgag tgccccgccg atgcccgcca aggaggtcgt
     7261 tcccgagttc gggttccgcg aggctcccgc cgcggtggca gcggccgccc tcgccgctat
     7321 tcgcgaaggc ggagccaagc ccgcgtcgcg gggccgcgga cgcggaggcg cgcgcccgga
     7381 gccggccatc gaggcaatgg cgcccggaac ggaggagccg cccgagcctc cgactgcggc
     7441 tgaagcagct gcggat
//
LOCUS       DATCQR010000169         9786 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_164220, whole genome shotgun sequence.
ACCESSION   DATCQR010000169 DATCQR010000000
VERSION     DATCQR010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 9786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9786
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164220"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..659
                     /locus_tag="VIN32_05640"
     CDS             <1..659
                     /locus_tag="VIN32_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013284172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HEY8922096.1"
                     /translation="IEECLNPPVEWDGATNAWITTEPFSAPEAFPFPEGIGPIECVNV
                     EHEEVLLVPRYLKGVRRVTFKYALGSDFIAKLQFLHDIGLDRTEPVRVRGVDVSPRDL
                     VAAVVPDPITMGDKMVGRAVVGTWVIGAKDGKPREVFLYQMADAAETLRTTGSQVVGW
                     QTGFNPVIAMELLASGAWSGSGVHAPEAFDPDPYLALMDGYGIHHAMLEMEPGAHRPT
                     "
     gene            complement(663..938)
                     /locus_tag="VIN32_05645"
     CDS             complement(663..938)
                     /locus_tag="VIN32_05645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8922097.1"
                     /translation="MRRLPAGPAAARLDGLVLLLVAVTAAGGLGLLVGGARPRELLHF
                     VYAVVALAALPISTSVSARWEPRRRGMATVIGALVGLAVIVRLFGTG"
     gene            complement(1028..2182)
                     /locus_tag="VIN32_05650"
     CDS             complement(1028..2182)
                     /locus_tag="VIN32_05650"
                     /inference="COORDINATES: protein motif:HMM:NF012263.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HEY8922098.1"
                     /translation="MTPAGGSLEAEAPQSGRPGVLARLSDQFGFRQLVTDYLIPVESN
                     SIWYLLGGVLAISLGLEIATGFLLSLVYIPDAAKAYGITQHLLATGGWSFVLNFHFFN
                     SFLIFGLVMIHMLRVFITGGYRRGKEGLWLVGVALAGLTFVISLTGEALHWDEVGFGV
                     PWNISEVLSAVGLSGVFNYTSDALLSIPTATEKLVQIYVLHVSIVPIGLGLFIAWHYL
                     LIRFKGISVPFWMRASGRTSGFSEHIRGWLLWGGLIIGIVVLIAIFVPRDAGTAPQLL
                     PSSSLYGVDDDPGGLGFKPTYPISWTRGMNIIAANLGIDPDIWGAMIAMALLLGALLV
                     IPFVDRGPHEPDSSASAFDLRKRGWAFLAMGIFWLVLIAGLIQNAIVAEG"
     gene            2368..2715
                     /locus_tag="VIN32_05655"
     CDS             2368..2715
                     /locus_tag="VIN32_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008479173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein"
                     /protein_id="HEY8922099.1"
                     /translation="MNVDEMLTGARDVMNVKRVYGDPIERDGILVIPAAKVAGGGGGG
                     GDSENNAGGGFGVRATPAGAWIIRDGQVAWEPAVDATRLATLAMVVAIVFLLTVRSVA
                     KANARAREKTARR"
     gene            complement(2770..2844)
                     /locus_tag="VIN32_05660"
     tRNA            complement(2770..2844)
                     /locus_tag="VIN32_05660"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2810..2812),aa:Met,seq:cat)
     gene            2959..4488
                     /locus_tag="VIN32_05665"
     CDS             2959..4488
                     /locus_tag="VIN32_05665"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEY8922100.1"
                     /translation="MAVNPKKLRPYDQPPKVRRPILPNEPDNAATAFLVAALVWFLAA
                     TAIGLLWVAYQLFPDQLTLRLQMPTFSGVLNFELSPATTTSGFWNSLVYGWLGNAGLG
                     AILFITPRLLGRRLASETAASAAAFLWNIGLIAGLVAIYVPQLTGSGALTEFPLPVAG
                     VLLLALLLANAAFWRTVLASAERLPYVSIWYFGVALLALLGLYSLISLAQLLAVVNLF
                     SLSPTGMALANAFYGRALETLCLGGMAIGTLYYVIPRATANPLYSWGLAMLAWLVWLG
                     LSTLAPLARLVDASVPFAITQIGNVATFLLVLPAFLVVANLLLSLSGRWTLVLSPGTV
                     PFALVALTFLVANALLEAIGSLGSVRALVGSTEWGTGVRLFALLGTATFAFLALGDHA
                     FPRLLRRDWGGTLLAEATLWAAFAGAALAGLALIGGGVVHGSLLAQAATPKEIDGFVF
                     WFELVLGAGIGLSALAAAVLALNVFLMYTSARRADYAIVDASADASSAAVEPAAAPAA
                     Q"
     gene            4493..4957
                     /locus_tag="VIN32_05670"
     CDS             4493..4957
                     /locus_tag="VIN32_05670"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEY8922101.1"
                     /translation="MTIHTPIESRTRGIPGWALTLTGVMVLAGALFFATNLKGSNPPI
                     LGTPQPSGSGGGLNAQSIIKAAGCQACHGPDLAGQAAFPTLHGVKNGPISKNLQQLGK
                     DHPDDWANLWIAGTDPALAGIARNGMPVFAGPKTNLSPEEIATVVEYLKTLP"
     gene            4954..5808
                     /locus_tag="VIN32_05675"
     CDS             4954..5808
                     /locus_tag="VIN32_05675"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HEY8922102.1"
                     /translation="MTDPAERPVRGVTVLIGGDEMARIDEAAQGLGLSPDALMESAGA
                     AVTEVALAELVSMSEPVTGPGGPLAPAPLTVVMCGPGNNGGDGFVAARRLASAGRPVT
                     AVLVGDPARISGVAAAHNWAVLQAMASSGSLSLFVAPTPELLLQLRARLSPASLLIDA
                     LLGSGAAGPLREPVSSAVDLLNAIVTHARAAGRPCRVLAIDTPTRIDLTDGTRSDPVV
                     VADLTVTFHRAKAGFAVDPEARRLAGRYFVAPIGIPLEAEAGIVPPDGEFPPARMTQV
                     NWQEPAVS"
     gene            complement(5801..6457)
                     /gene="pyrE"
                     /locus_tag="VIN32_05680"
     CDS             complement(5801..6457)
                     /gene="pyrE"
                     /locus_tag="VIN32_05680"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEY8922103.1"
                     /translation="MEPVPVATRSPEVRLPQGEALTNAGVEELFEVAGALRRGHFLLK
                     SGRHSDRYLEKFAVLQYPALAVEIGRRLAASLMNRDPTLVVGPTTGGALLAAETARQL
                     EAALGRTVRGVFAEPMERGTRALRRGWPVTVADRVVLVDDILTTGGSLAETVRAVRAA
                     GATPLLASVIVDRSTGPVEIGCPILSLGRIEISSWTADECPACQRGEALVKPGSSAAS
                     "
     gene            complement(6462..7283)
                     /locus_tag="VIN32_05685"
     CDS             complement(6462..7283)
                     /locus_tag="VIN32_05685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8922104.1"
                     /translation="MSAPRISALRELAGEAELERRDFVYEATEQLRRFLAANRERLLE
                     IGPIKLVDDEQDYLLFDPIDEVWTTRLTYQDPADSEWYDEEQTVEAVSEVVELYNLAD
                     ILSWFVDAARDPTNHLVSADKLAAHEAVAEEPHDEDWQAGLSTTQQETIAAQRLWQLA
                     DAYRTQIGDQQKQVLREFAANAEEVLKQTGDLVLLDEETDTLVLRADGRFETSLDLSD
                     MPNGTALKEDPHRMGLTVTDARTVADVYDPTDVLQWVTDALTERYPAVDFTAVYE"
     gene            complement(7280..7606)
                     /locus_tag="VIN32_05690"
     CDS             complement(7280..7606)
                     /locus_tag="VIN32_05690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8922105.1"
                     /translation="MPLMVTFFNVRKGRDALFKRGMRHFFPRTVKRYEAEYGIRFIGW
                     FNVTEGSEWNNVLILDLPTYSVLDRLYQDPAARGLGHRVAESIFDKKHIIFLRERMGP
                     DLVYKP"
     gene            complement(7607..7987)
                     /locus_tag="VIN32_05695"
     CDS             complement(7607..7987)
                     /locus_tag="VIN32_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8922106.1"
                     /translation="MWDHADRPPRSERWMSIRTNEELDPNEGPGFGCFVWQAILFAGL
                     LFLIPIGLFTFNWPVWLLVVLLFIDMVLLFFVSMTSVFLLRVFFADRRRGRGRTVGPA
                     APTRVITTQPPEQDEPPTNEGEAE"
     gene            complement(7995..9383)
                     /locus_tag="VIN32_05700"
     CDS             complement(7995..9383)
                     /locus_tag="VIN32_05700"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEY8922107.1"
                     /translation="MSRFGSLRNPAATFLLSGARVIDPSAGEDEVRDLAVVEGQLVEP
                     AALPPDAPRLDGSGLVVAPGLCDLHAHLREPGLERAETIESGARAAAHGGFTTICAMP
                     NTDPPVDDPEGVSRVLERASGAACRVRVIAAATRERGGEQPTDLAALAPLGIAGVSDD
                     GSAVSSERAARDLLARLAPFGLPLIEHAEDPGLATGSVMRAGPTATRLGLAGWPASAE
                     LSIVERDIALAEETGGWVHFTHLSTASGLDAIRRARERGVRVTCDVTPHHLALTDAWV
                     AGWRGFAWEEPTGVAADPFGAPLDATLAYDAACRVNPPLAPRAEALALTAGVADGTID
                     AIATDHAPHPRERKAVEFGAAAPGMIGLETALSLGLAAVEAGRLELVRLLGSLSTGPA
                     SLIGEVRTLRPGSLADLVVFDPAARWRVEASGLASASSNTPLLGMELPGVVRMTVASG
                     RITYRDGLAAVD"
     gene            complement(9380..>9786)
                     /gene="pyrB"
                     /locus_tag="VIN32_05705"
     CDS             complement(9380..>9786)
                     /gene="pyrB"
                     /locus_tag="VIN32_05705"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:NF012412.4"
                     /note="catalyzes the transfer of the carbamoyl moiety from
                     carbamoyl phosphate to L- aspartate in pyrimidine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEY8922108.1"
                     /translation="ARSNLHSLTALGASVWVAGPPAWVAGFEGWPGVTVANRLEEALA
                     DADAVMALRIQLERDAGGGVTSLREYTAEWGLDEARMSLASPGALLLHPGPTNEGVEI
                     TADLAAGKRSLIGTQVANGVPIRMAVLSLLAR"
BASE COUNT         1525 a         3372 c         3368 g         1521 t
ORIGIN      
        1 cgatcgagga gtgcctcaac ccaccggtcg agtgggacgg cgcgacgaac gcctggatca
       61 cgaccgagcc attcagcgct cccgaggcat tcccgttccc cgagggaatc ggcccgatcg
      121 aatgcgtcaa cgtcgagcac gaagaggtgc tcctggtgcc tcgctacctg aagggggtgc
      181 ggcgggtcac cttcaagtac gcgctgggca gcgacttcat cgccaagctg cagttcctcc
      241 acgacatcgg cctcgaccgc accgagccgg ttcgcgtccg cggtgtcgac gtctcgccac
      301 gcgacctggt cgcggcggtc gtgcccgacc cgatcaccat gggcgacaag atggtcggcc
      361 gggcggtggt cgggacctgg gtcatcggcg caaaggatgg caagccgcgc gaggtgttcc
      421 tgtaccagat ggccgatgcc gccgagacgc tgcgcaccac cggctcgcag gtggtcggct
      481 ggcagaccgg cttcaacccg gtgatcgcga tggagcttct tgccagcggg gcctggtctg
      541 gaagcggagt gcatgccccc gaggcgtttg atcccgaccc gtatcttgcg ctgatggacg
      601 gctacgggat ccaccacgcg atgctcgaga tggagcccgg ggcgcatcgc ccgacctgac
      661 ggtcagccgg tcccgaagag ccgcacgatg accgccagcc cgacgagggc cccaatgacc
      721 gtcgccatcc ctcggcgccg cggctcccaa cgcgccgaga ccgatgtcga gatcgggagc
      781 gctgcgagcg cgacgacggc gtagacgaag tggagcagtt cgcggggacg cgctcctccc
      841 acgaggagtc caaggccgcc ggcggcggtt accgcgacga gaaggagcac gagcccgtcg
      901 agccgagccg ctgccggccc agccggcagg cgtcgcaagg cgcgctgggc accctcgaga
      961 ccagcgagca gaaccagcag tccgctgccg atcgccagcc agctatggag cgctgctggg
     1021 attgtcgtca gccctcggcg acgatcgcgt tctggatgag gcctgcgatc aggacgagcc
     1081 agaagatccc catggcgagg aaggcccagc cgcgtttccg taaatcgaag gcggaagcgg
     1141 agctgtccgg ctcgtggggg ccgcgatcga cgaaggggat gacgagcagc gcgccgagca
     1201 acagagccat ggcgatcatg gcgccccaga tgtccgggtc gatccccagg tttgccgcga
     1261 tgatgttcat gccccgggtc cagctgatcg ggtaggtcgg cttgaagccg aggccaccgg
     1321 ggtcatcgtc gaccccatac agcgacgatg acggcagaag ctgcggcgcc gtccctgcat
     1381 cacgcgggac gaagatcgcg atcaggacga cgatcccgat gatcaggccg ccccacagca
     1441 gccagccgcg gatgtgctcg ctgaagcccg aggtacggcc gctggcgcgc atccagaagg
     1501 gcaccgagat gcccttgaac cggatcagca gatagtgcca ggcgatgaac aggccgaggc
     1561 cgatcggcac gattgaaacg tgaaggacgt agatctgtac cagcttctcg gtcgcggtcg
     1621 ggatcgacag cagggcgtcg gaggtgtagt tgaagacacc cgagagcccg acggcgctga
     1681 ggacctcgga gatattccac gggacgccga accccacctc gtcccagtgc agggcctctc
     1741 cagtcagcga gatgacgaag gtcaggcctg ctagggctac gccgaccagc cacaggcctt
     1801 ccttgccgcg ccgataccca ccggtgatga acacgcgcag catgtggatc atcaccagcc
     1861 cgaagatcaa gaacgaattg aagaagtgga agttgaggac gaatgaccat ccgccggtgg
     1921 cgagcaggtg ctgggtgatg ccgtaggcct tcgcggcgtc ggggatgtac acgagagaca
     1981 gcaggaagcc ggtggcgatc tccaggccca ggctgatggc gaggacccct ccaaggaggt
     2041 accagatgct gttcgactcg acggggatca gatagtcagt gaccagttgg cggaacccga
     2101 attggtccga gagcctcgcc aacacgccag gccggccgct ctgtggggcc tctgcttcga
     2161 gagacccgcc ggcgggagtc atgaccttct ccttgcagct gttggcgtcg ctgggcgacg
     2221 tgccaacctg attatctccc cgcgtcaatg gcgagattgg gctcgtcgcg cttctgccaa
     2281 cagtgccgaa tggcccaatc ttcctgcccg catccggagc gatgcgtatc gggccgtaga
     2341 ctggactccc acaccaagga gagacccatg aacgttgacg agatgctgac gggcgcccgc
     2401 gatgtgatga atgtcaagcg ggtctatggc gatccgatcg aacgcgacgg gatcctggtc
     2461 attcccgcgg ccaaggtggc tggcggcggc gggggaggcg gcgacagcga gaacaatgcc
     2521 ggaggcggat tcggtgtgag ggccacgcct gccggagcgt ggatcatccg cgatgggcag
     2581 gtcgcctggg agccggcagt ggatgccacc cgcctggcaa ccctggccat ggtggtggcg
     2641 atcgtcttcc tgctgaccgt ccgctcggtc gccaaggcca acgccagggc acgcgagaag
     2701 accgccaggc gctgagccgg acgcgcgaac gccgcctggc gggaggcggc gttcggttct
     2761 ggaggtcttt ggttgcgggg gacggattcg aaccgacgac cttcgggtta tgagcccgac
     2821 gagctaccac tgctccaccc cgcccggcca tgctaaaggc cgcgtcggga tcggtcaaac
     2881 ctttggcgtc cgctacaatg ccgccgggtt cgcgccgctc tgcgacccgt cccgatcgat
     2941 gcgaggctgc gcacgccgat ggccgtgaac ccgaagaagc tgcgacccta cgaccagcca
     3001 ccaaaggtcc gtcgcccgat ccttccgaac gagccggaca acgctgcgac cgcattcctg
     3061 gtggcggcgc tggtctggtt cctggccgcc accgcgatcg ggctgctgtg ggtggcctat
     3121 cagctcttcc cggaccagct caccctccgc ctccagatgc ccaccttcag tggggtgctg
     3181 aacttcgagc tgagcccggc caccacgacc tcaggcttct ggaactcgct ggtctacggc
     3241 tggctcggca acgccggcct gggcgccatc ctgttcatca ccccacgtct tctcggccgg
     3301 cggctggcga gcgagacggc agcctctgcc gcagcgttcc tctggaacat cggcctgatc
     3361 gccggtctcg tcgcgatcta cgtcccgcaa ctcaccggct cgggggcgct caccgaattc
     3421 cccctgccgg tggccggggt cctgctgctg gctctcctcc tcgccaacgc cgcgttctgg
     3481 cgaaccgtgc tggcgtcggc cgagcggctg ccatacgtga gcatctggta cttcggcgtg
     3541 gcactcctgg cgctgctcgg cctgtacagc ctgatcagcc tcgcgcagct gctcgcggtc
     3601 gtgaacctgt tcagcctctc cccgaccgga atggcgctcg ccaatgcctt ctacggccga
     3661 gcgctggaga ccctgtgcct gggcggcatg gccatcggca cgctgtacta cgtcatcccg
     3721 cgcgccactg cgaacccgct ctactcatgg ggcctggcga tgctcgcctg gctcgtctgg
     3781 ctcgggctga gcaccctggc gcccctcgcc aggctggtgg acgcgtcggt cccgttcgcg
     3841 atcacgcaga tcggcaacgt ggcgaccttc ctgctcgtgc ttcccgcctt cctggtcgtc
     3901 gcgaacctgc tgctcagcct cagcggccgt tggaccctcg tcctctcgcc ggggaccgtc
     3961 ccatttgcgc tggtggcgct cacattcctg gtggccaacg cgctgctgga ggcgatcggc
     4021 tcgctcggct cggtgcgggc actggtcggg agcacggagt gggggaccgg cgtccgcctg
     4081 ttcgccctgc tcggcacggc caccttcgcg ttcctggcgc tcggcgatca cgccttcccg
     4141 cgcctgctgc gacgggattg gggagggacc ctgctcgcgg aggcgacgct gtgggctgct
     4201 ttcgcgggtg ccgcgctggc gggtctggcg ctgatcggcg gtggcgtggt gcacggctcc
     4261 ctgctggcgc aggcggcgac gcccaaagag atcgacggct tcgtgttctg gttcgagctg
     4321 gtgctcgggg cgggaatcgg cctcagcgca ctggctgcag cggtgttggc actgaacgtg
     4381 ttcctgatgt acaccagcgc acgccgcgcc gactacgcca tcgtggacgc gtccgctgac
     4441 gcatcctctg ccgcggtcga acccgctgcc gcgccggcgg cccagtgagc cgatgacgat
     4501 ccataccccg atcgaatcgc ggacgcgcgg catccccggc tgggcgctga ccctgacggg
     4561 ggtcatggtc ctcgccggcg cactcttctt cgccaccaac ctgaaggggt cgaatccacc
     4621 catcctcggc acgccgcagc cgtcgggatc gggtgggggc ctcaatgctc agtccatcat
     4681 caaggcggcc ggctgccagg cctgccacgg cccggacctg gcggggcagg ccgccttccc
     4741 gaccctgcac ggcgtgaaga acggcccaat cagtaagaac ttgcagcagc tgggcaagga
     4801 tcaccctgac gactgggcca acctctggat tgccgggacc gatccggcgc tggccgggat
     4861 cgcccgcaac ggcatgcccg tcttcgccgg cccgaagacg aacctcagtc ccgaggagat
     4921 cgccaccgtg gtcgagtacc tgaagaccct gccatgaccg atccggccga acggccggtg
     4981 cgcggggtga cggtcctcat cggtggcgac gagatggcgc gcatcgatga ggcggcgcag
     5041 ggccttggcc tgtcacccga tgccctgatg gagagcgcag gcgcggccgt cacggaagtg
     5101 gcactcgcgg agctcgtctc gatgagcgag ccggtgaccg gccccggtgg gccgctcgcg
     5161 ccggcgccgc tgaccgtcgt gatgtgcggc ccgggcaaca atggcggcga cggcttcgtg
     5221 gcggctcggc gccttgcgtc ggcgggacgg ccggtgaccg cggtcctggt gggtgatccc
     5281 gcgcggatca gcggagtcgc cgcggcacac aactgggccg tgctgcaggc gatggcatcg
     5341 agcggctcgc tgtccctctt cgtggctccc acgcccgagc tgctgctcca attgcgagcg
     5401 cggctctcgc cggcgagcct gctgatcgat gccctgctcg gcagcggtgc ggccggcccc
     5461 ttgcgcgagc cggtctccag cgcggtcgac ctcctcaacg cgatcgtgac ccatgcccgg
     5521 gccgccggcc ggccgtgtcg ggtactcgcc atcgacacgc cgactcgcat cgacctgacc
     5581 gatggcaccc gctcggaccc ggtggtggtc gccgacctga ccgtcacatt ccaccgggcg
     5641 aaggccgggt tcgcggtcga ccccgaggcg cggcgcctgg ccggccgcta cttcgtggcg
     5701 ccgatcggca tcccactgga ggcagaggca gggatcgtcc cgcccgacgg cgagtttcct
     5761 ccggcgcgca tgacgcaggt gaactggcag gagcccgccg tcagctagcg gcgctgctcc
     5821 ccggcttgac gagtgcctca ccgcgttggc aggcggggca ttcgtcggcg gtccaggacg
     5881 agatctcgat cctccccagc gacaggatcg ggcagccgat ctcgaccgga cccgtggagc
     5941 ggtcgacgat cacgctcgcc agcaggggtg tcgccccggc cgcgcggacc gctcggaccg
     6001 tctcggccag cgatccgccg gtggtcagga tgtcatccac cagcacgacg cggtcggcga
     6061 cggtgacggg ccagccgcga cgcagcgcgc gagttccccg ctccatcggc tcggcgaaga
     6121 cgccacgcac cgtgcggccc agtgcggcct ccagctggcg tgcggtctcg gcggcaagca
     6181 gggccccgcc ggtggtcggt ccgaccacca gggtcgggtc gcgattcatc agggaggcgg
     6241 ccagccgccg cccgatctcg accgccagcg cggggtactg gagcaccgcg aacttctcga
     6301 ggtagcgatc cgagtggcgt ccgctcttga gcaggaagtg cccgcggcgc agtgcgccgg
     6361 cgacctcgaa cagctcctcg actccggcat tggtcagcgc ttcgccctgg ggaaggcgga
     6421 cctctggcga gcgggtggcg accggcacag gctccatcgg gctattcgta gacggcggtg
     6481 aagtcgaccg cgggatagcg ttcggtgagg gcatcggtca cccattgcag gacatcggtc
     6541 gggtcgtaga cgtccgcgac ggtgcgggca tcggtgacgg tcaggcccat gcgatgcggg
     6601 tcctctttga gggcggtccc gttcggcatg tcggagaggt cgaggctcgt ctcgaatcga
     6661 ccatccgcgc gcaggaccag ggtgtcggtc tcctcgtcga gcagcaccag gtcgccggtc
     6721 tgcttcagca cctcctcggc gttggcggcg aattcacgca gcacctgctt ctgctggtcg
     6781 ccgatctggg tccgatacgc gtccgccagc tgccagagac gctgcgcggc gatcgtctcc
     6841 tgctgggtcg tcgacaggcc ggcctgccag tcctcgtcgt gcggctcctc ggcgaccgcc
     6901 tcatgcgctg cgagcttgtc ggccgatacc aggtggttcg tcggatcgcg agcagcgtcc
     6961 acgaaccagg agaggatgtc ggccaggttg tagagctcca caacctcgct caccgcctcg
     7021 accgtctgct cctcgtcata ccactcgctg tccgcggggt cctggtaggt caggcgcgtg
     7081 gtccacactt cgtcgatggg gtcgaacagg aggtagtcct gctcgtcgtc gaccagcttg
     7141 atcggaccga tctccagcag gcgctcccgg ttcgcggcca gaaagcggcg cagctgctcg
     7201 gtggcctcgt acacgaagtc tcggcgctcg agctcggcct cgccggccag ctcgcggagg
     7261 gcggagatgc gcggcgcgct catggcttgt agaccaggtc cggccccatc cgctcgcgca
     7321 ggaagatgat gtgcttcttg tcgaagatcg actccgccac ccgatggccc aggccgcgcg
     7381 cggcgggatc ctggtagagg cggtcgagca cggaatacgt cggcagatcg aggatcagca
     7441 cgttgttcca ttccgaccct tcggtgacat tgaaccagcc gatgaagcgg atcccgtatt
     7501 ccgcctcgta gcgcttcacc gttcgcggaa agaagtggcg catcccgcgc ttgaagagcg
     7561 catcgcggcc cttgcggacg ttgaagaagg tgaccatcag gggcatctac tcggcctccc
     7621 cctcgttcgt cggcggctca tcttgctcag gcggttgcgt ggtgatgacc cgggtcggag
     7681 cggcgggacc gacggtccgt ccgcgacccc gacgacggtc ggcgaagaag acgcgcagca
     7741 ggaagacgct ggtcatgctg acaaagaaga gcaggaccat gtcgatgaag aggagcacga
     7801 ccagcagcca gaccggccag ttgaaggtga acaggccgat ggggatgagg aagaggaggc
     7861 cggcgaacag gatcgcctgc cagacgaagc acccgaagcc tggtccctca ttcggatcga
     7921 gttcctcgtt ggtgcggatc gacatccatc gctcgctgcg gggtgggcgg tccgcatggt
     7981 cccacatccg gcgctcagtc gacggcggcg agtccgtcgc ggtaggtgat tcgcccgctc
     8041 gccaccgtca ttcggaccac gcccggcagc tccattccca gcagcggcgt gttcgacgag
     8101 gccgacgcca ggccggatgc ctcgacccgc catcgggcgg cgggatcgaa gacgaccagg
     8161 tcggcgagcg atcctggccg gagggtccgg acctcgccga tgagcgaggc agggccggtg
     8221 ctcagcgatc ccagcagtcg caccagctcc agcctgcctg cctcgactgc cgccaggccc
     8281 agcgacagcg ccgtttcgag cccgatcatc cccggcgctg cggcgccgaa ctcaaccgcc
     8341 ttccgttcac gcggatgggg ggcgtgatcc gtggcgatgg cgtcgatcgt cccgtccgcc
     8401 acgccggccg tgagggcgag cgcctcggcg cgtggggcga gcggcgggtt cacccggcag
     8461 gcggcgtcgt aggcgagcgt cgcatccagc ggagctccga acggatcggc ggcgacgccg
     8521 gtcggctctt cccatgcgaa gccacgccaa ccggcgaccc aggcatcggt cagggcgagg
     8581 tgatgcgggg tgacgtcgca ggtcacccgg acgccgcgct ccctggcacg acgaatggca
     8641 tccagcccgc tcgccgtgga gagatgcgtg aagtgcaccc atcctccggt ctcctccgcc
     8701 agggcgatgt cgcgctctac gatcgacagt tccgcgctcg ccggccagcc ggcgaggcca
     8761 aggcgtgtgg ctgtcggacc ggcgcgcatc accgaaccgg tcgcgaggcc gggatcctcg
     8821 gcatgctcga tcagcggcag gccaaatggg gcgaggcgcg ccaggaggtc acgcgccgcg
     8881 cgttcggagc tgacggcgct cccgtcgtcg ctcacgcctg cgatgccgag cggggccaac
     8941 gccgcgagat ccgtcggctg ttcaccgcca cgctcgcggg tggctgcggc gatcacgcgc
     9001 acgcggcacg ccgccccgct cgcccgctcc agcacccggc tcacgccttc gggatcgtcg
     9061 acgggcggat cggtattcgg catggcacag atggtcgtga aaccgccgtg agccgcggct
     9121 cgtgcgccgc tctcgatcgt ctcggcccgc tccaggccag gctcgcgcag atgggcgtgc
     9181 aggtcgcaca atcccggcgc aaccaccagg ccgcttccgt cgaggcgcgg tgcgtcgggg
     9241 ggaagcgcgg cgggttcgac cagctgccct tcgaccacag ccaggtcgcg aacctcatcc
     9301 tccccggctg acggatcgat cacccgcgcc ccgctcagca gaaacgtcgc ggccgggttc
     9361 ctgagcgacc cgaatcggct catctggcca gcagggagag gaccgccatg cggatcggga
     9421 cgccgtttgc aacctgcgtc ccgatcaggc tgcgcttgcc cgctgccagg tccgcggtga
     9481 tctcgactcc ctcgttggtc gggccgggat gcaggagcag cgcacccgga ctcgccaggc
     9541 tcatgcgggc ctcgtcgaga ccccactcgg ccgtgtattc ccggagcgag gtgacgccgc
     9601 ctcccgcgtc gcgttcgagc tggatgcgca gcgccatcac cgcatcggca tcggccaggg
     9661 cctcctccag ccggtttgcg acggtgaccc ccggccagcc ctcgaatcct gcgacccacg
     9721 cgggcggccc ggccacccat accgatgcac cgagcgcggt cagggaatgc aggttcgagc
     9781 gtgcca
//
LOCUS       DATCQR010000170         4272 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_55968, whole genome shotgun sequence.
ACCESSION   DATCQR010000170 DATCQR010000000
VERSION     DATCQR010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4272
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55968"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            128..847
                     /locus_tag="VIN32_05710"
     CDS             128..847
                     /locus_tag="VIN32_05710"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY8922109.1"
                     /translation="MAVRTTRHAGLGRARAGQLSHRFGSELRIARTTAGLTQAQLARL
                     AGVSQSVVSRAERATRRIEWSTACALASATGHELSLRLFPADGVRLRDRGQLQAVQAI
                     VTDAHPSWHPSVEQPVGLRDRRAADLVLRGPHESIHIEVERGLVDLQAQLRAAQLKRA
                     ALVEQFGQAVRLVIAVPGTRRSRRLVGDLLLTFRTALPASSRAVWASIRSGSPLGADG
                     ILFLPQNHHRLEPQSADGTGD"
     gene            complement(978..1853)
                     /gene="ispH"
                     /locus_tag="VIN32_05715"
     CDS             complement(978..1853)
                     /gene="ispH"
                     /locus_tag="VIN32_05715"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEY8922110.1"
                     /translation="MSTAVRPEVRIAERAGFCYGVRLAIDKAKHARDEGKEVTTLGQL
                     VHNPGIKADLSDRGIRTADLADQVEGGTLVIRAHGVPANELERVRGKPELEVIDATCT
                     WVLQSQKAARELAAAGYTVCIIGHEDHPEVKGVRSYAGEGHVVVDDMDRSTWERVPHT
                     KKIGVLSQSTILPFKLEEFAAFCLRRCHDLRVVNTVCPVTLTRQADSVVLAAEVDAMI
                     VVGGKNSSNTKELAVKCAEVCPATVHVADADELEAEHGEWVRGKARIGITGGTSTPEV
                     DLEEIRDRIYALTAA"
     gene            complement(1831..2523)
                     /locus_tag="VIN32_05720"
     CDS             complement(1831..2523)
                     /locus_tag="VIN32_05720"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEY8922111.1"
                     /translation="MSTWFYFPGAVMTGWVKWLLGGGRVEGLANAPRSGPFIMVSNHA
                     SNFDPPLIGAAIGRHIGRPIHFMAKEEINGWPLIGWLARGAGVFFVRRGQGDRSAQRL
                     ALEHLAAGRPIGIFPEGTRSRTGVLREPKAGVALLAMRSGAPLLPVGISGSGKIFPGR
                     SRWPHRTRVDIRIGKPFRLPHQPSGRLDRDALTDGTERIMREIAALLPEWQQGRYRPG
                     PIDLGNEHSRST"
     gene            complement(2520..3968)
                     /gene="cmk"
                     /locus_tag="VIN32_05725"
     CDS             complement(2520..3968)
                     /gene="cmk"
                     /locus_tag="VIN32_05725"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEY8922112.1"
                     /translation="MPTERIQKLLARSGVASRRGAELLIAAGRVTVNGRTARLGDAAD
                     PAADRLAVDGAPLPAASQTVHYAVHKPIGLLSSAHDERGRRSVVSLVDVDTDAHLWPA
                     GRLDVDSEGLMVLTNDGDWANRVLHPRYGVEREYAALVDPVLTPDDMDALVAGVELDD
                     GPARLLAIQLAPPPPEVTRSTPERGRWLRLRVGEGRKHEVRRLFAAGGYRVERLVRTR
                     LGSLSLDGLREGEWRALRPAEVEAMAGARPKVRAADPRKPLTVAIDGPSGSGKSTVGY
                     AVAQRIGATFVDTGLMYRALTLAALERGVDPDDGEALGRLAREVRIEVRRPRHEQTDR
                     RETVLLDRRDVTREARTPQVDRVVSSVSRHAAVRDAMLHVQRGAARRRDTVMVGRDIG
                     TVVLPDATLKVFLTAAAAVRAARRAAEMGRPDRLDRYLAEIEERDAADIGREVAPLRK
                     APGALVLDTGDLDVDPCVDAIVGHLPPQQSGP"
     gene            complement(3958..>4272)
                     /gene="scpB"
                     /locus_tag="VIN32_05730"
     CDS             complement(3958..>4272)
                     /gene="scpB"
                     /locus_tag="VIN32_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEY8922113.1"
                     /translation="EEARLTPAALEALAVVAYRQPCTRGEVERVRGVDSDYVLRSLLH
                     RRLLVEMGRRDTPGRPILYGTTFAFLERFGLTSLDDLPPIEDGSAPTLLAAEEQEPVD
                     AD"
BASE COUNT          600 a         1552 c         1477 g          643 t
ORIGIN      
        1 gcgcagcggc acctctcgta caagctgctg cgacgccggc tgcggcgcct cctcgagcca
       61 gccgagtcgc tagcgagcta agcgctgggt catcggctgg taagcgcccg tcgcgcatgg
      121 ttgcgggatg gcggtacgga cgacccggca cgccggcctc ggccgtgccc gggccggtca
      181 actctcccat cgcttcgggt ctgagctgcg cattgcccgc accaccgccg gtctaaccca
      241 ggcgcaactg gcgcgcctcg ccggtgtcag ccagtccgtg gtcagcaggg ccgaacgtgc
      301 gacccggcgg attgagtggt cgaccgcctg cgcgctcgct tcagcgaccg gccacgagct
      361 cagcctcagg ctctttcccg cggacggcgt ccgactccgg gatcgcgggc agctccaggc
      421 cgttcaggcc atcgttactg acgcccatcc ttcgtggcat ccgagcgtcg agcagcccgt
      481 gggccttcga gaccgacgcg cggccgatct cgtcctccgc ggtccccacg aatcgatcca
      541 catcgaggtc gagcgggggc tcgtcgacct gcaggcccag ctgcgcgcgg cacagctcaa
      601 acgggcggcg ctggtcgagc aattcggtca ggctgtgcgc ctcgtcattg cggtgcccgg
      661 tacgcggcgg tcccgccgcc tggtcggtga cctgctgctg accttcagga ccgcgctgcc
      721 ggcaagctct cgagccgtct gggcctcgat ccgcagcggc tcaccgttgg gtgccgacgg
      781 catcctcttc cttccccaga atcatcatcg gcttgaaccc caatcggccg atggcacagg
      841 ggactaggaa agacccattg gcgttgaatg agtcggttga tgcccccagt ggactaggga
      901 cgactggcaa actgccattc cgcgtcgaac gtagtccggg ccgccatagc caggtccgct
      961 ggccggccgt gaagcggtca ggcggcggtc agggcgtaga tccggtcgcg gatctcctcg
     1021 aggtcgactt cgggggtcga ggtcccgccg gtgatcccga tccgcgcctt gccgcgcacc
     1081 cactcgccgt gctcggcctc gagctcgtcg gcgtcggcga cgtggaccgt ggcggggcac
     1141 acctcggcgc acttgacggc gagctccttg gtgttgctgc tgttcttgcc gccgaccacg
     1201 atcatggcat ccacctcggc ggccagcacc accgaatcgg cctggcgcgt gagcgtcacc
     1261 ggacacacgg tgttgacgac gcgcaggtcg tggcagcgtc ggaggcagaa tgcggcgaac
     1321 tcctcgagct tgaaggggag gatcgtcgac tgcgacagga ccccgatctt cttggtatgc
     1381 ggcacccgct cccaggtgga tcgatccatg tcgtccacga ccacgtgtcc ctcgccggca
     1441 tacgagcgca cgcccttcac ctcgggatgg tcctcgtgac cgatgatgca cacggtgtag
     1501 ccggcagcgg cgagctcgcg ggccgccttc tgcgactgca ggacccaggt gcaggtggcg
     1561 tcgatcacct cgagctccgg cttgccgcgc acccgctcca gctcgttggc gggaaccccg
     1621 tgggcgcgga tgacgagggt tccgccctcg acctggtcgg cgaggtctgc cgtgcggatg
     1681 ccgcggtcgg agaggtcggc cttgatgccg gggttgtgca ccagctgtcc gagggtggtg
     1741 acctccttgc cctcgtcgcg cgcgtgcttg gccttgtcga tcgccaggcg aacgccgtag
     1801 cagaagccgg cacgctcggc gatgcggacc tcaggtcgaa cggctgtgct cattccccaa
     1861 gtctatcggg ccgggccgat atcgaccctg ctgccactcg ggcaggagcg ccgcgatctc
     1921 ccgcatgatc cgctccgtcc cgtccgtcag cgcgtcgcgg tcgagtcgcc cgctgggttg
     1981 gtgcgggagc cgaaatggct tcccgatgcg gatgtcgacg cgggtgcggt gcggccatcg
     2041 gctgcgcccg ggaaagatct tgccgctgcc gctgatcccg accggcagga gcggcgcgcc
     2101 actccgcatg gcgagcagcg cgacccccgc tttcggctct cgcaagaccc cggttcgcga
     2161 gcgggtcccc tccgggaaga tcccgatcgg gcggccggcg gccaggtgct cgagggcgag
     2221 ccgctgcgcg ctccggtctc cctgaccacg gcgcacgaag aagacgcccg ccccgcgggc
     2281 cagccagccg atcagcggcc agccattgat ctcctccttg gccatgaagt ggatcgggcg
     2341 accgatgtga cgcccgatcg cggcgccgat caggggcggg tcgaaattgg aggcgtggtt
     2401 gctgaccatg atgaacgggc ccgagcgtgg agcgttcgcg aggccctcga cccggccgcc
     2461 accgagcagc cacttcaccc acccggtcat caccgcgccg gggaagtaga accaggtgct
     2521 catggcccgg actgctgggg cggcaggtgc cccacgatgg cgtccacaca ggggtcgacg
     2581 tccaggtccc cggtgtcgag caccagcgcc ccgggcgcct tgcgcagcgg cgccacctcg
     2641 cggccgatgt ccgccgcgtc gcgctcctcg atctcggcca ggtagcgatc gagacgatcc
     2701 ggccgcccca tctcggcggc gcgccgcgcg gcacgcaccg ccgcggcggc ggtcaggaac
     2761 accttcagcg tagcgtcggg caggacgacg gtgccgatgt cgcggcccac catcaccgtg
     2821 tctcggcgtc gcgccgcgcc tcgctggaca tgcagcatcg cgtcacggac ggctgcgtgg
     2881 cggctgaccg acgagacgac gcggtcgacc tgcggcgtgc gggcctccct ggtcacgtct
     2941 cgccggtcaa gcagcactgt ctcgcggcga tcagtctgct cgtgccgggg gcgccgaacc
     3001 tcgatccgca cctccctggc caggcgaccc agcgcctccc cgtcatcggg gtccacgccg
     3061 cgctcgaggg ctgcgagcgt cagggcccgg tacatgaggc cggtgtccac gaaggttgca
     3121 ccgatgcgct gcgccactgc atacccgacg gtgctcttgc cggagccgga gggcccatcg
     3181 atggcgaccg tgagcggctt gcgcgggtcg gcggcccgga ccttcggtcg agcgcccgcc
     3241 atcgcctcca cctcggccgg gcgcagggcg cgccactcgc cctcgcgcag gccatccagc
     3301 gacagcgacc cgaggcgcgt ccgcaccagc cgttcgacgc gatagccgcc ggcggcgaag
     3361 agacgcctga cctcgtgctt gcgaccctca cccactcgca gccgcagcca gcgaccgcgc
     3421 tcgggggtgg atcgggtcac ctccggcggg ggcggtgcaa gctggatcgc caggagtcgc
     3481 gcaggcccgt cgtcgagctc gacccctgcc acgagcgcat ccatgtcgtc gggcgtcaga
     3541 acgggatcga ccagcgccgc gtactcgcgc tcgaccccgt aacgcgggtg gaggacgcgg
     3601 ttggcccagt cgccgtcgtt cgtcagcacc atcaggccct cggaatccac gtccagcctg
     3661 cccgccggcc agagatgcgc atcagtgtcg acgtcgacca ggctgacgac cgagcgacgg
     3721 ccgcgctcat cgtgcgccga cgagaggagc ccgatcggct tgtgcaccgc gtagtggacg
     3781 gtctgcgacg cggccggcaa gggcgctccg tcgacggcca gccggtccgc tgcgggatcg
     3841 gcggcgtcgc ccagtcgtgc tgtcctccca ttgaccgtga cacggcctgc cgcgatcagg
     3901 agctcggccc cgcgccgcga ggccacgccg ctgcgcgcca gcagcttctg gatccgctca
     3961 gtcggcatcg accggctcct gctcctcggc agccagcagc gtgggtgcgc tgccatcctc
     4021 gatgggaggc aggtcgtcga gggaagtgag cccgaagcgt tccaggaagg cgaaggtggt
     4081 cccgtacagg atcggacggc cgggagtgtc cctccggccc atctcgacca gcagccgccg
     4141 atgcaggagg gatcggagca cgtagtcgga gtcgacgccg cgcactctct ccacctcgcc
     4201 gcgggtgcac ggctggcggt aggccaccac cgccagcgcc tcgagcgccg ccggcgtgag
     4261 gcgcgcctcc tc
//
LOCUS       DATCQR010000171         4264 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_195153, whole genome shotgun sequence.
ACCESSION   DATCQR010000171 DATCQR010000000
VERSION     DATCQR010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4264
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195153"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..4175
                     /locus_tag="VIN32_05735"
     CDS             <1..4175
                     /locus_tag="VIN32_05735"
                     /inference="COORDINATES: protein motif:HMM:NF027518.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HEY8922114.1"
                     /translation="IYLFNPGTIFDSAVWGQVDSVGTLVLLATLYMLARGWTEAAAAG
                     AVLGMLVKFQFGFLIPLVAVVGIKRHVFGRSSDPDLAGRADPIRVLTSLATGLGTLVL
                     LILPFRLAIWAPADPTLSLVDRFIAASNTYKGLTVNAFNLWRNPLTGLGDVYRWGCDS
                     LPPTCADGSGVAFTLGSTPISWQLVGALLFGLAALVALWTVARRDDPAGLLVGALVLA
                     VAFFALPTRVHERYLFPALALAAPLVARSWKWAVIYAVLTLSFFANVYWLYTTDFSFA
                     GGDHPMNPGIGRLPMARDPVVASIVFNDTGIYLLSLMIVIAMAWLLTRAATMALGVRE
                     EGVSQVPAPVEVAPPRPASAIVLAGVPEPRKSRLPAWLRRDPSYREDPPRRLDRLDLV
                     LLVAIVLVAFFFRLWRLEIPRHTHFDEVYHARSATEWLADWQEGWTRDTYEWTHPPLA
                     KYLIAAGIVLADPNKVVGGTDLDAPATAMAVAPQRTAQTRPESIVFTSAGDEVITARE
                     VLTGKKVASWSAEGPVASLAYDEDDNRLLVGLASGGAVSSYDLKAFLGQLGERGPPPG
                     IGQIDTGLAGVTQIVIPDSQTVILFAGPNGIVEVERKTGVELASTELVAAGVGYVPGG
                     SGDVADPRVVAVDQAGSGLIVLDGATLKPATDVAGSDGVIPLPSAPAGPLLVQGSGKS
                     LQIWVPVGPLPADDEHGPVKGGLSVFDESVKLIDTVPLPGAATAIGWQRVANIVYVAG
                     IEERTGQPVVWAVQPLGNGGTQSAGFATFDTTVLPGKALAMGFDISDDSQGDDHELLL
                     VSTDLGDGGRLASIDAGSNAFAWRIAGIVFGAALVGIIYLFAATLFSRRRIAVLAAVF
                     VAFDGMSYVMSRIAMNDIYAATFIVAGYALFWQIWSGRWARSAWWVLPLVGVMIGLAA
                     ATKWVGWYALIGLWVLVLARSEFGRFLLVAGIAFLTIVAGFGAPWPFLVVCILALVLA
                     LLLVWRRPIRLSPQDILALPPIGIVGGGIGLAFAIAYSTVDGREPRSAVEFIFAFLAR
                     GAQAAWPAWIMLAVAGLLLAARAVRSLRTPASDRRWYIPGELGGFAWPWVVACLLVLP
                     LLVYFIAYIPYLELGHGIATQHLGPGYGWSLDEMQSQMFGYHFGLQAGHPAASPWWSW
                     PLDLKPVWFYSHSYDDRLMAVIYNGGNPILFWAGIPAIGWCAVQAWRHRSLALTLIVA
                     AFAFQYLPWTRIERATFHYHYFTAVLIAMIGVAYLVDVGLRSWAYRSLAIAFLVLAVI
                     AGLLIFPLGSALAMPDWYINAARALAPWNYAFKFPDPPQGDRGQLLSADSLKLAIGLV
                     VSLGAAAFALFGRGFFTARRAAARPVGRDSDPSGWAPPDRSEEDGLDDNEPHEPEADE
                     DHPRDPGPAESA"
BASE COUNT          541 a         1528 c         1433 g          762 t
ORIGIN      
        1 tcatctacct cttcaacccc ggaaccatct tcgactccgc cgtctggggc caggtcgact
       61 cggttgggac gctggtactg ctcgcgacgc tgtacatgct cgcccgcggc tggaccgagg
      121 ccgccgcggc gggtgcggtg ctcgggatgc tggtcaagtt ccagttcggc ttcctgatcc
      181 cgctcgtcgc cgtggtcggc atcaagcgcc atgtcttcgg ccgctcgtca gaccccgacc
      241 tggcgggccg ggcggacccg atcagggtcc tcacctcgct cgcgaccggc ctcggcaccc
      301 tggtcctgct gatcctcccg ttccgcctcg ccatctgggc ccctgccgat ccgacgctga
      361 gcctggtcga ccgcttcatc gcggcgagca acacctacaa gggcctgacg gtcaacgcct
      421 tcaacctgtg gcgcaacccc ttgacgggcc tcggcgacgt gtaccgatgg gggtgcgact
      481 ccctcccgcc cacctgcgct gatggatcgg gcgtggcctt caccctcggc tcgaccccga
      541 tcagctggca gctcgtcggc gccctgctgt tcggcctcgc cgccctggtc gcactgtgga
      601 ccgtcgccag gcgcgacgac cccgccggcc tgctggtggg ggcgctggtc ctcgccgtcg
      661 ccttcttcgc gctgccgacc cgcgtccacg agcgctacct cttcccggca ctcgccctgg
      721 cggctcctct ggttgcccgg tcgtggaagt gggcggtcat ctacgccgtg ctgaccctct
      781 cgttcttcgc gaacgtgtac tggctgtaca ccaccgactt ctcgttcgcg ggcggcgacc
      841 atcccatgaa tcccggtatc ggcaggctgc cgatggcgcg cgacccggtc gtcgcctcaa
      901 tcgtcttcaa cgacacgggg atctacctac tgtcgttgat gatcgtgatc gccatggcct
      961 ggctcctgac ccgggcggcg acgatggcac tgggcgtgcg cgaagaaggg gtctcgcagg
     1021 tgcccgctcc agtcgaggtg gcgcccccgc gcccagcgtc cgcgatcgtg ctcgccggcg
     1081 tcccggagcc gcggaagtcg cgccttcccg cctggctgcg ccgcgacccg tcctaccgcg
     1141 aggacccgcc ccgacgcttg gatcggctcg acctggtcct gctggtggcc atcgtccttg
     1201 tcgcgttctt cttccgcctg tggcgcctcg aaatcccacg gcacacccac ttcgacgagg
     1261 tctaccacgc ccgctccgcg accgagtggc tggccgactg gcaggagggc tggacacgcg
     1321 acacctacga atggacccac ccgccgctgg cgaagtacct gatcgccgcc ggcatcgtgc
     1381 tggccgaccc gaacaaggtc gtcggcggaa ccgatctcga cgccccggcc acggccatgg
     1441 cggtggctcc ccagcgaacg gcccagaccc gacccgagtc gatcgtcttc acttcggccg
     1501 gggacgaggt gatcacggca cgcgaggtgc tgaccggcaa gaaggtcgca tcctggagcg
     1561 ccgaaggccc ggttgccagc ctggcgtatg acgaggacga caaccgcctg cttgtgggtc
     1621 tcgcatcggg cggagcggtc tcctcgtacg acctgaaggc gttcctcggc cagcttggcg
     1681 agcgcgggcc accgcccggc atcgggcaga tcgacaccgg cctggccggc gtgactcaga
     1741 tcgtcattcc ggacagccag accgtgatcc tcttcgccgg ccccaacggg atcgtcgagg
     1801 tcgagcgaaa gacaggcgtc gagctggcga gcaccgagct ggtggcggcc ggggttggct
     1861 acgtgcccgg gggatcggga gatgttgccg acccacgggt ggtggccgtc gaccaggcgg
     1921 gttccgggct gatcgtgctc gacggcgcga cgctgaagcc cgcgaccgac gtggcaggca
     1981 gcgatggcgt gattccactt ccgtcggctc cggcgggtcc gctgttggtg caggggagcg
     2041 gcaagagcct gcagatctgg gtgcccgtcg gcccgctgcc cgctgacgac gagcacggac
     2101 cggtcaaggg tgggctcagc gtcttcgacg agagcgtgaa gctgatcgac acggtcccgc
     2161 ttccgggcgc cgcgacggcg atcggctggc agcgggtcgc caacatcgtc tacgtcgcgg
     2221 ggatcgagga gcgaaccggt caaccggtcg tgtgggccgt ccagccgctc ggcaacggcg
     2281 ggacgcagag cgcaggcttc gccaccttcg acaccaccgt gctccccggc aaggccctgg
     2341 cgatgggctt cgacatctcg gacgattccc agggcgacga ccacgagctg ctcctcgtct
     2401 ccaccgacct gggcgatggc ggacgcctgg cgtcgatcga tgccggctcg aatgcgttcg
     2461 cctggcgcat tgcgggcatc gtctttggag cggccctggt cgggatcatc tacctgttcg
     2521 ccgcgaccct cttctcgcgc cgacggatcg ccgtgctggc ggccgtgttc gtggccttcg
     2581 acgggatgag ctacgtcatg agccggatcg ccatgaacga catctacgcg gccaccttca
     2641 tcgtggcggg ctatgcgctc ttctggcaga tctggtcggg gcgctgggcg cgcagcgcct
     2701 ggtgggtgct cccgctggtc ggggtcatga tcgggctggc cgcggcgacc aagtgggtcg
     2761 gctggtatgc cctcatcggc ctgtgggtgc tggtgctggc ccgatccgag ttcggccgct
     2821 tcctgctggt ggcggggatc gccttcctca ccatcgttgc gggcttcgga gcgccctggc
     2881 cattcctggt cgtctgcatc ctggcgctcg tgctggcgct cctgctggtc tggcggcggc
     2941 cgatccggct gtcgccgcag gacatcttgg cgctgccgcc gatcgggata gtgggcggcg
     3001 gcatcggcct tgccttcgcg atcgcctact ccaccgtgga cggccgtgaa ccgcgcagcg
     3061 ccgtcgagtt catcttcgcc ttcctggcgc gcggtgccca ggccgcctgg ccggcctgga
     3121 tcatgcttgc cgttgcgggc ctgttgctgg cggcccgcgc ggtgcgctca ctgcggactc
     3181 cggccagcga ccggcgctgg tacatccccg gcgagctggg tggcttcgcc tggccgtggg
     3241 tcgtcgcctg cctgctggtg ctgccgctgc tggtgtactt catcgcctac atcccatacc
     3301 tggagctggg gcacgggatc gcgacccagc acctgggccc cggctacggc tggtcgctgg
     3361 acgagatgca gtcgcagatg ttcgggtatc acttcggcct gcaggccggg cacccggctg
     3421 cctcaccgtg gtggagctgg ccgctcgacc tgaagccggt ctggttttac agccactcct
     3481 acgacgaccg gctgatggcg gtcatctaca acggcggcaa cccgatcctg ttctgggccg
     3541 gcatcccggc catcgggtgg tgcgcggtgc aggcctggcg tcaccgatcg ctggcgctca
     3601 ccctgatcgt cgccgccttc gcgttccagt acctgccctg gacccgcatc gagcgagcca
     3661 ccttccacta ccactacttc acggcggtcc tgatcgccat gatcggggtg gcctacctgg
     3721 tggatgtggg gctgcggtcg tgggcctacc ggtccctcgc gatcgccttc ctcgtgctgg
     3781 ctgtgatcgc cgggctgctg atattcccgc tcggatcggc gctggccatg ccggactggt
     3841 acatcaacgc cgctcgcgcg cttgccccat ggaactacgc cttcaagttc cccgatccgc
     3901 cgcagggcga ccggggccag ttgctcagcg ccgacagcct caagctggcg atcggcctgg
     3961 tcgtctcgct cggcgccgcg gcctttgccc tcttcgggcg cggcttcttc accgctcggc
     4021 gagcggccgc gcgtcccgtc ggccgggact ccgacccatc aggctgggcg ccgccggatc
     4081 gctccgaaga ggatggtctc gatgacaacg agccccacga gccagaggcg gatgaggatc
     4141 accctcgcga tccaggcccc gcggaatcgg cttgacacgg caggaaggcg ggccgtagac
     4201 tcccgcggcg gccccgacct tgccccactg ctgtgtggtg ccgcccggcc aacctctcaa
     4261 cgag
//
LOCUS       DATCQR010000172         2840 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_164585, whole genome shotgun sequence.
ACCESSION   DATCQR010000172 DATCQR010000000
VERSION     DATCQR010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2840
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164585"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..582)
                     /locus_tag="VIN32_05740"
     CDS             complement(<1..582)
                     /locus_tag="VIN32_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019854262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEY8922115.1"
                     /translation="MDDRASWAELAAQLRVDSIRSSSAAGSGHPTSSMSAADLMSVLL
                     SKYLHYDFDAPEDPRNDHLILSKGHASPLLYAAYKAAGAISDAEMLTFRKAGSRIEGH
                     PTPVLPWVDVATGSLGQGLPIAVGVALAGKQLDRLPYRVWCLCGDSEMAEGSIWEAFE
                     SAAFHGLANLTAIIDVNRLGQTGETMHGWDLDLF"
     gene            complement(683..1018)
                     /locus_tag="VIN32_05745"
     CDS             complement(683..1018)
                     /locus_tag="VIN32_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011309655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain containing protein"
                     /protein_id="HEY8922116.1"
                     /translation="MAYSDQVLSCADCGIEFVFSASEQEFFAQKGFTSAPKRCSSCRA
                     QRRATGGGGSYAASSGGGYNGGGGYSRGPREMFDAVCARCAKDTQVPFRPTGARPVYC
                     SDCFRLMRG"
     gene            1240..1428
                     /locus_tag="VIN32_05750"
     CDS             1240..1428
                     /locus_tag="VIN32_05750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8922117.1"
                     /translation="MRTVAAVLGLLLALGGAVWIAQGLNLPFAPRSFMTADRTWIVLG
                     FVAVLAGVATFTWARRRS"
     gene            complement(1439..2023)
                     /locus_tag="VIN32_05755"
     CDS             complement(1439..2023)
                     /locus_tag="VIN32_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HEY8922118.1"
                     /translation="MPSYDPSTALVVVDVQNDFADPAGSLYVRGGEEVVSRINDEIRL
                     ASSDGACVVYTQDWHPPSTPHFAKDGGVWPVHCVAETWGAELHPGLVVAGPSVRKGSN
                     GEDGYSGFTMRDPDSGEDRPTELAGLLTDQGVTRVVVGGLATDYCVLGTVLDARRLGF
                     ETVVLTGAVAAVEQTPGDGENALAQMAEVGAQLD"
     gene            2061..>2840
                     /locus_tag="VIN32_05760"
     CDS             2061..>2840
                     /locus_tag="VIN32_05760"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY8922119.1"
                     /translation="MAEERRLVTVLFADVVGSTSLGDGMDPEDLRALLSRFYGIASEV
                     TANHGGTLEKFIGDAALAIFGIPQAHDDDVRRAIAAGLELRDRVKADPVLGDRLPIRL
                     GLNTGEVMASRERSDGNFIVTGDAVNVAARLQQAAEPWQLLVTPRSAHAAGQAFSFGE
                     PMELMVKGKVGAVTALPVLGAAKAAVQRIPLVGREADLDQLLLVARRTFAERRPYLVS
                     VIAPAGTGKTRLLEEFLDRLGSVVPGVRVATAQCLPYGQRLT"
BASE COUNT          446 a          987 c          953 g          454 t
ORIGIN      
        1 gaacagatcc aggtcccagc catgcatcgt ctcgccggtc tggcccagcc gattcacatc
       61 gatgatggcg gtcaggttgg ccaggccgtg gaaggccgcc gactcgaacg cctcccagat
      121 cgagccctcc gccatctccg agtcgccgca caggcaccag acccggtatg gcagacgatc
      181 cagctgctta cccgcgagcg ccaccccgac cgcgatcggc agcccctgtc caagcgagcc
      241 ggtggccacg tcgacccacg gcaggaccgg ggtcgggtgg ccctcgattc ggctgccggc
      301 cttgcggaag gtgagcatct cggcgtcaga tatggcgccg gccgccttgt aagcggcgta
      361 cagcagcggc gaggcgtgac ccttgctcag gatcagatgg tcgttgcgcg gatcctcggg
      421 cgcatcgaag tcgtagtgca ggtacttcga cagcagcacg gacatgaggt cggcagccga
      481 catggagctg gtcgggtgcc cggagccggc cgccgagctg gagcggatcg agtcgacgcg
      541 gagctgtgca gcgagctcgg cccaggatgc tcgatcgtcc atgtcgggac catcataggg
      601 accgatcggc gcggcgaaaa atgaagaggc ggcaccccgc aatcgggacg ccgcctcggc
      661 cggccgcaag gccgatgtgg gactacccgc gcatcagccg gaagcaatcg ctgcagtaga
      721 ccgggcgcgc gccggtcggg cggaacggca cctgcgtgtc cttggcgcat cgggcacaga
      781 cagcgtcgaa catctcacgc ggtccacggc tgtagccccc gcccccgtta tacccaccgc
      841 cgctgctcgc ggcgtagctg ccgccgccgc ccgtggcgcg ccgttgggct cggcacgagg
      901 agcagcgctt gggcgcagag gtgaatccct tctgcgcgaa gaattcctgc tcggacgcgg
      961 agaagacgaa ttcgattccg cagtcagcgc agctgagcac ctggtcgctg tacgccacga
     1021 ggaccctccc acatagagtt gccacgtcgg gcgtctacgc ctcgatcagc gccggccacg
     1081 ggtcttgccg ctaccccgtg agccgacgac ggacgtggtg ggagggggag ggaaagctgc
     1141 tggcgccgct ctcatgcggc ccgacttcgg ccgagtatac cgacagattt cccccacgca
     1201 agaggtcaaa ttcggcccga tccggctatc ctgacgggca tgcgaacggt tgctgctgtg
     1261 ctcggcctgc tgctcgccct cggcggtgcg gtgtggatcg cgcaggggct gaacctgccg
     1321 ttcgcgccgc gcagcttcat gaccgcggat cggacctgga tcgtcctcgg attcgtggcg
     1381 gtgctcgcgg gggttgcaac cttcacctgg gcgcggcgcc gttcatgacc acggcccgct
     1441 agtcgagctg cgcacccacc tcggccatct gcgccagggc gttctcccca tcgccggggg
     1501 tctgttcgac agccgccact gcccccgtca gcacgacagt ctcgaagccg agccggcgcg
     1561 cgtcgaggac cgtgcccagc acgcagtagt cggtcgcgag cccgccgacg acaaccctcg
     1621 tcactccctg gtctgtcagc agccccgcga gctcggttgg acgatcctcg cccgagtcag
     1681 ggtcgcgcat cgtgaagccc gagtagccgt cctccccgtt gctccccttg cggacgctgg
     1741 gtccggccac caccaacccg gggtgcagct cggcgcccca cgtctcagcg acgcagtgca
     1801 ccggccagac gccgccatct ttggcgaagt gcggagtcga cggcggatgc cagtcctgcg
     1861 tgtagaccac gcaggcacca tcgctcgacg ccagccgaat ctcgtcgttg atccgcgaca
     1921 ccacctcttc tccgccccgc acgtagaggc tgccggctgg atcggcgaag tcgttctgga
     1981 cgtcgaccac cacgagcgcg gtcgatgggt cgtagcttgg caccccttca tcgtagcggc
     2041 gggtcggtac catggccgcc atggccgagg agcgccgcct ggtgaccgtc ctgttcgccg
     2101 acgtggtcgg atccacctcg ctgggcgacg gaatggaccc cgaggatctg cgcgcgctcc
     2161 tctcgcgctt ctacgggatc gccagtgagg tcaccgccaa tcatggcggg acgctcgaga
     2221 agttcatcgg cgatgcggca ctcgccatct tcggcatccc acaggcccac gacgatgacg
     2281 ttcgacgtgc gatcgccgcc ggcctcgagc ttcgagatcg ggtcaaggct gatcctgtcc
     2341 tcggcgatcg gctccccatc cggcttggcc tgaacaccgg cgaagtgatg gcgagccgcg
     2401 aacggtccga cggcaacttc atcgtgaccg gggatgcggt gaatgttgcc gcccgactgc
     2461 agcaggccgc cgaaccatgg cagctgctcg tcacgccacg cagcgcgcat gccgccggcc
     2521 aggccttctc gttcggcgag ccgatggagc tgatggtcaa ggggaaggtg ggcgcggtta
     2581 ccgcgctccc ggtgctcggc gccgccaagg ccgctgttca gcggattccc ctggttggac
     2641 gcgaggcgga ccttgatcag ttgctgctcg tcgcgcgccg gacgtttgcg gagcgacgcc
     2701 cgtacctcgt gagcgtgatc gcaccggcgg ggaccggcaa gacaaggctg ctcgaggagt
     2761 tccttgatcg cctcggcagc gtcgttccag gcgtgcgcgt ggccacggcg cagtgtctgc
     2821 cgtacgggca gcgattgacc
//
LOCUS       DATCQR010000173         5667 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_164691, whole genome shotgun sequence.
ACCESSION   DATCQR010000173 DATCQR010000000
VERSION     DATCQR010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5667
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164691"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..519)
                     /locus_tag="VIN32_05765"
     CDS             complement(<1..519)
                     /locus_tag="VIN32_05765"
                     /inference="COORDINATES: protein motif:HMM:NF013707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HEY8922120.1"
                     /translation="MTTRIALDGVGGQGVRVIAGVLGALLARMGKQVTVLFDYDSSVR
                     GSMSDAFVIFDDAPIANPVVEEADIMLKLADKGHLRISGRKVVTDLGLGRPGDEQIPF
                     ASLGSEHFGKELFGNMIALGRLLRLAEIDFDDAAIRESLPRRYQDENVAAIRFGYQLT
                     PEEIARLAAAAPP"
     gene            complement(516..1874)
                     /locus_tag="VIN32_05770"
     CDS             complement(516..1874)
                     /locus_tag="VIN32_05770"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEY8922121.1"
                     /translation="MPEAPFAELGFKRHLKVDLFPTIWCPGCGIGTIMIELAMVLDEM
                     GLDETNTILVTGIGCTGRMGSYMKFESVYTLHGRTLPVAEAIKAVRPEMHVVVVAGDG
                     DTASIGGNHLIHAIRRNAPLTVLCNNNEIYGLTGGQTGPTTPTGTKTLSSPAGNPNTP
                     INLQGLVRSSAHALYAKTTVYHQLHMRNAIREAIEHPGFSFVDITSQCIENNGRRIGF
                     ASANDMLNFYRKTYKRAAKDAEHLNPFEIGILAPRPADAVSTNGNGAGTTSTPERPAD
                     LPSQPPGPMASATESPGALESTGAALPTSDDDKARRRAESQERAAVMAQLSAEVKLAV
                     AKKELTLTDALTQAGIPIPEWLRTGASAPSGAPVASGEVQISATGQDPHTERQPTELD
                     AQKAEAAVEVDPREAKRAEAQRKLALMQRLPSDVKLRVAKGEITLADALREAGIDPES
                     IG"
     gene            complement(2127..3263)
                     /locus_tag="VIN32_05775"
     CDS             complement(2127..3263)
                     /locus_tag="VIN32_05775"
                     /EC_number="1.2.7.3"
                     /inference="COORDINATES: protein motif:HMM:NF013974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HEY8922122.1"
                     /translation="MRKLTQGNEAVFRGAIAAGASYYAGYPISPSTEILNVASGWAAE
                     HPEFRFLQAEDEIASANAVIGASLAGAKAFTATSGPGFSLMQEAVGYAQKVGVPCVFV
                     NVQRVGPATGMPTMPGQGDIMQVRYGSTGDYTPIAFYPNGVEEAFRVTVQAFNAAEQS
                     LSPVVVLSDTFVAHLNEVVDLDEVAASVEVAERTLVPLGRHEAGKPRFFAGVVMYEQG
                     PNAGEPATADADEYLRQYYDAKHRHTEVAKRYALYEHGWNADAKVLIVCYGIVSRVAS
                     PLSTEYALFRPIRIHPMLSEELRAIADRYEQVVCIEANDGQYADMVELALHREVKRVP
                     LLGGRISLEAVRDGLDRVLAGGSSEPPIPAAPTEAVERSPLGIG"
     gene            complement(3503..3802)
                     /locus_tag="VIN32_05780"
     CDS             complement(3503..3802)
                     /locus_tag="VIN32_05780"
                     /inference="COORDINATES: protein motif:HMM:NF024585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEY8922123.1"
                     /translation="MTRQGASDPIGFGSGISDDHKDGIRWVDYSNISWNPVFCKRCDI
                     CVEICPKNTLVLRNDAIIEEQDCILCGLCERYCPDLAIEMLPEAVAAHAAREKGT"
     gene            complement(3853..5442)
                     /locus_tag="VIN32_05785"
     CDS             complement(3853..5442)
                     /locus_tag="VIN32_05785"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEY8922124.1"
                     /translation="MNPLATVRCVMAVAVLTIFVGCASPSPSLVPSPSAIPTPSPSPT
                     ASPIPDVTFPLAVVTGLTNLKATITLDELGTLASGGQLVVPCEVTLTQPAITATAPCV
                     AANQVAVDLRHDQTLVALVPPGLVEPATKVLSIAGDGPFGLFGPDLFGDPESRALPYP
                     IMGSAHPWALDSSWTAYDASQVWNLTSIGSLCADRVAAYQAVTLGKGWDWPFNGGTAT
                     YMGGPFLNPNPPAGISRFPIVKPVESANEGVAASISKRSDVALADHKCPILPDAQWVP
                     NQTGPPSLAVPEAVLTRWDNFLGIDAVFLAADHQSDRGVNGISSTLKILDAHGLPHTG
                     LGMNLDQALEPAYVEAAGLKIAFVSWNEVTGPAHAGPTTPGVAWLEQANVDAAVGKAR
                     AAGADLVICDPQWWGGDEYHPDLWASQLAAVHWMDGARCDQIIGGGLHIAGGIFLGVT
                     QYGLRLIDAGPGNYMYGQPWWQQTQEGVILDMTFRGTTLVNVRLRPYVMILNARPALT
                     DPEGDGHYVLERVWKASEVAY"
     gene            complement(5475..>5667)
                     /locus_tag="VIN32_05790"
     CDS             complement(5475..>5667)
                     /locus_tag="VIN32_05790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8922125.1"
                     /translation="VNPVVAVALGVAFRGETLTPRSVLAAALIIGAVVAMVSGRPRKI
                     EEGGPSPETASLESEAKAQ"
BASE COUNT          849 a         1872 c         1996 g          950 t
ORIGIN      
        1 cggtggggcg gcggccgcca gtcgcgcgat ctcctcgggg gtgagctggt agccgaagcg
       61 gatcgcggcc acgttctcgt cctggtagcg acgcgggagc gattcgcgga tcgccgcatc
      121 gtcgaagtcg atctccgcca ggcgaaggag ccggcccaac gcgatcatgt tgccgaacag
      181 ctccttgccg aagtgctccg agccgagtga tgcgaacggg atctgctcgt cgccgggtcg
      241 gcccaggccc aggtcggtca cgaccttgcg gccgctgatg cgcaggtgcc ccttgtcggc
      301 cagcttgagc atgatgtccg cctcctcgac caccggattg gcgatcggtg cgtcgtcgaa
      361 gatgacgaag gcgtccgaca tgctgccgcg cacgctggag tcgtagtcga acagcacggt
      421 gacctgcttg cccatgcggg ccagcagcgc gccgagcacc ccggcgatga cgcgcacgcc
      481 ctgcccacca acgccgtcga gcgcgatgcg cgtcgtcacc cgatcgactc ggggtcgatg
      541 ccggcctccc gcagcgcgtc ggcaagggtg atctctccct tggcgacccg cagcttcacg
      601 tcggagggaa ggcgctgcat aagggccagc ttgcgctgcg cctctgcgcg ctttgcctcg
      661 cggggatcga cctctaccgc cgcctccgcc ttctgcgcat cgagctcggt cggctgccgc
      721 tccgtgtgcg gatcctggcc ggtggcgctg atctgcacct cgcctgacgc cacgggcgcg
      781 ccggaagggg cactcgcgcc ggttcgcagc cactcgggga tcgggatacc cgcctgcgtc
      841 agcgcatcgg tcagggtcag ctccttcttc gcgaccgcca gcttgacctc tgcggacagc
      901 tgagccatga ccgcagcccg ctcctgggat tcggctcgcc gccgcgcctt gtcgtcgtcg
      961 ctcgtcggca gagcggcccc ggtggactcc aacgcgccgg gtgactcggt cgcggacgcc
     1021 atcggtccag ggggctgcga aggcagatcc gccgggcgct cgggcgtgct ggtggttcct
     1081 gcgccattcc cgttggtgct gaccgcatcg gccggtcgcg gggccaggat cccgatctcg
     1141 aaggggttga ggtgctcggc gtccttggcg gcacgcttgt aggtcttgcg gtagaagttg
     1201 agcatgtcgt tggcgcttgc aaagccgatg cgtcgcccgt tgttctcgat gcactggctg
     1261 gtgatatcga cgaagctgaa gcccgggtgc tcgatcgcct cgcggatggc gttgcgcatg
     1321 tggagctggt ggtagaccgt cgtcttggcg tacagcgcgt gcgcggatga tcggaccagg
     1381 ccttgcagat tgatcggtgt gttcgggttg cccgcgggcg aggagagggt cttggtgccg
     1441 gtcggggtgg tcgggccggt ctgcccgccg gtcaggccgt agatctcgtt gttgttgcac
     1501 aggacggtca gcggtgcgtt gcggcggatg gcgtggatca ggtggttgcc gccgatgctg
     1561 gccgtgtcgc cgtcccccgc caccaccacc acgtgcatct ccggccgaac ggccttgatg
     1621 gcctcggcga ccggcagggt gcggccgtgg agcgtgtaga cgctctcgaa cttcatgtag
     1681 ctgcccatcc ggccggtgca gccgatgcct gtgaccagga tcgtgttcgt ctcgtccagg
     1741 cccatctcgt cgaggaccat cgccagctcg atcatgatcg tgccgatgcc gcagcccggg
     1801 caccagatgg tcgggaaaag gtcgaccttc aggtgccgct tgaagccgag ctcggcgaag
     1861 ggcgcctcag gcactggccg atcgctccgc ggccgcccgc agttgccaaa tcgcaacctg
     1921 gctggcccta ggcgtggtcg attgctcttc gcgccggcgc aggtcgctgt gctgagcacg
     1981 aataattggc cgcgacaagg cggacgggca gctcctgtcg cctaagagca gtctcaatgt
     2041 gatctggcga ccaattggca ttggtcgctc ggtagcgaca accaggttgc gatttggcga
     2101 cgacatgcgt gactgaggcg tcggcatcag ccgatcccca gcggcgagcg ctcgaccgcc
     2161 tcggtgggag cagccgggat cggcggctcc gaggaaccgc cagcgagcac gcggtcgagc
     2221 ccgtcgcgca ccgcttccaa gctgatccgc ccgccgagca gggggacgcg cttgacctcg
     2281 cgatgcaatg ccagctcgac catgtcggca tactgcccat cgttcgcctc gatgcacacg
     2341 acctgctcat agcgatcggc aatggcgcgc agctcctcgc tgagcatcgg gtggatccgg
     2401 atcgggcgga acagggcgta ctccgtggag agcggcgagg cgacccggct gacgatgccg
     2461 tagcagacga tcagcacctt cgcgtcagcg ttccagccat gctcgtagag cgcataccgc
     2521 ttggcgacct cggtgtgccg atgcttcgcg tcgtagtact ggcgcaggta ttcatcggcg
     2581 tcggcggtgg ccggctctcc cgcgttcggc ccctgttcgt acatgaccac gcccgcaaag
     2641 aagcgcggct tgcccgcctc gtgcctgccg agcggcacga gcgtgcgctc cgcgacctcg
     2701 acgctggctg cgacctcgtc gaggtccacc acctcgttga ggtgcgccac gaacgtgtcg
     2761 gacaggacca ccaccggtga gagcgactgc tccgcggcgt tgaaggcctg gacggtcacg
     2821 cggaacgcct cctcgacccc gttcgggtag aaggcgatcg gggtgtagtc gccggtgctg
     2881 ccgtaacgca cctgcatgat gtcgccctgg ccgggcatgg tcggcatgcc ggtcgccggg
     2941 ccgacccgct gcacgttcac gaagacgcag ggcacgccca ctttctgggc gtagccgacc
     3001 gcctcctgca tcaggctgaa gccaggcccg ctggtggcgg tgaacgcctt cgccccggca
     3061 aggctcgcgc cgatgacggc gttcgcgctg gcgatctcgt cctccgcctg gaggaaccgg
     3121 aactccggat gctcggccgc ccagccggag gcgacgttga ggatctcggt ggacggggag
     3181 atcgggtacc ccgcgtagta gctggcaccc gccgcgatgg ctccgcggaa gaccgcctcg
     3241 ttgccctggg tcagcttccg catcggcctg aggctgtcgt cttcacgccc ggcaacggga
     3301 ttcgtgccaa attcacgcgt ggcgggcgtg aagcgatggg accgggccgg gctcccgcca
     3361 aatgggcgcc acggtgtgaa tttggcacgt caaacggtct ccgtggtcat ctggtgtgaa
     3421 tctggcacac gtgagggcgg ttcgcccgtg tcacggtcac gcgatgtgaa tctggcaagg
     3481 agctgacaac gagaccgggc ggtcacgtgc ccttctctcg tgccgcgtgg gcagcgaccg
     3541 cctccggcag catctcgatg gcgaggtcgg ggcagtaccg ctcgcagagg ccgcacagga
     3601 tgcagtcctg ctcctcgatg atggcgtcgt tgcgcaggac gagcgtgttc ttggggcaga
     3661 tctcgacgca gatgtcgcag cgcttgcaga agaccgggtt ccagctgatg ttcgaatagt
     3721 cgacccagcg gatcccgtcc ttatggtcgt ccgagatccc ggagccgaag ccgatcgggt
     3781 ccgatgcgcc ttggcgagtc atgggagggc gattctacgc gggtcggggg tgcgcgtctg
     3841 cggcgggtcc ggctagtaag cgacctccga agccttccag actcgctcga ggacgtagtg
     3901 gccgtcgccc tccgggtcgg tgagggccgg gcgggcgttg aggatcatga cgtaggggcg
     3961 aaggcgcaca ttcacgaggg tcgtgccgcg gaaggtcatg tcgaggatga ccccctcctg
     4021 cgtctgctgc caccacggct gcccgtacat gtagttgccc ggtcccgcgt cgatgagcct
     4081 gagaccgtac tgggtcacgc cgaggaagat gccccccgcg atatgcaggc cgccgccgat
     4141 gatctggtcg cagcgggccc cgtccatcca gtgcacggcc gcgagctgcg acgcccacag
     4201 gtccgggtga tactcgtccc cgccccacca ctgggggtcg cagatgacca ggtcggctcc
     4261 ggcggcacgc gccttgccaa cggcggcgtc gacgttcgcc tgctcgagcc aggcgacgcc
     4321 cggcgtggtc ggcccggcgt gggcggggcc cgtcacctcg ttccacgaga cgaacgcgat
     4381 cttcagcccg gcagcctcga cgtacgcggg ctcgagggcc tggtcgaggt tcatgccgag
     4441 gccggtgtgc ggaaggccat gggcgtcgag gatcttgagc gtggagctga tgccgttgac
     4501 gccccgatcg ctctgatggt cggccgcgag gaacacggcg tcgatgccca ggaagttatc
     4561 ccacctggtc aggaccgcct caggcaccgc caggctcggc gggccggtct ggttcggcac
     4621 ccactgcgcg tcaggcagga tcgggcactt gtgatccgcg agggcgacgt cggatcgctt
     4681 gctgatgctc gcggcgaccc cctcgttggc actctcgacc ggcttgacga tcgggaatcg
     4741 cgagataccg gccggcgggt tcgggttgag gaacggcccg cccatgtagg tcgccgtccc
     4801 gccgttaaac ggccaatccc agcccttgcc aagggtgacg gcctggtacg cggcgacacg
     4861 atcagcgcac aggctcccga tcgaggtcag gttccagacc tgcgaggcgt cgtatgcggt
     4921 ccagctcgag tcgagcgccc aagggtgcgc cgaacccatg atcgggtacg gaagcgctcg
     4981 ggactctggg tcgccgaaca ggtccggtcc gaagagcccg aacggcccgt cgcccgcgat
     5041 cgagaggacc ttggtcgccg gctcgacaag gcccggcggc accagcgcaa cgagcgtctg
     5101 gtcatggcga aggtccacgg cgacctgatt cgccgcaacg cagggcgcag tcgccgtgat
     5161 cgccggctga gtcagggtta cctcgcatgg caccacgagc tggccgccgc tggcgagcgt
     5221 gccaagctcg tcgagggtga tcgtcgcctt gaggttcgtg aggccggtca cgacggcgag
     5281 cgggaaggtg acgtcgggga tggggctggc cgtcggagaa ggggacggcg tcgggattgc
     5341 gctcggcgac ggcacgagac tggggctcgg actcgcgcat ccgacgaata tcgtcaagac
     5401 cgcgacggcc atgacgcatc ggaccgtcgc cagggggttc acggcgccat cgtagccacc
     5461 ggcaggcagc cacactactg ggccttggcc tccgactcga gagacgccgt ctcgggtgaa
     5521 gggccgccct cctcgatctt gcgtgggcgt ccgctgacca tcgccaccac ggcaccgatg
     5581 atcagggcgg cggccaacac cgagcgcggg gtgagcgtct cgccacggaa tgccacgccg
     5641 agcgccaccg ctaccaccgg attgacg
//
LOCUS       DATCQR010000174         3611 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_102535, whole genome shotgun sequence.
ACCESSION   DATCQR010000174 DATCQR010000000
VERSION     DATCQR010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3611
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102535"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..472)
                     /locus_tag="VIN32_05795"
     CDS             complement(<1..472)
                     /locus_tag="VIN32_05795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8922126.1"
                     /translation="MEQLLDPLLSGADESMVNAFTSGFRVTQIKQAIAKALPEPAAAA
                     ALESAQVVALAILVREQLYPLQFAAIYRPFATVLPGPGYEPPPSVDRQIAAFLQKLPS
                     LSGEAEAEVAAVAKVLGEPPKAVPAVGAIGAVPAPPTDALDPSGLYAAAWKLAIA"
     gene            complement(589..1599)
                     /gene="glpX"
                     /locus_tag="VIN32_05800"
     CDS             complement(589..1599)
                     /gene="glpX"
                     /locus_tag="VIN32_05800"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003998263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HEY8922127.1"
                     /translation="MDQEMDRNLALELLRVTEAGALAAAPLQGRGDKMGADGRAVEAI
                     RSALASVDMKGTVVIGEGEKDEAPMLYFGEEIGNGLEPELDVAVDPIEGTSLTAAGLP
                     GAISVIALAERGTMFTTHIHYMQKLVVGRRAKGVIDLNMPVEWNLIRIAKAEGLPPQE
                     LTVVVLERPRNDGIIAEIRDVGARIKLIPAGDVGGALEAALEMRYGIHCMMGSGGATE
                     GVLAACAIKAIGGDMQAKLLLRDDDERDLAIKEGHQPDKVLSIDNLCSGNNIFFAATG
                     ITSGELLKGVRYDVVYAYTQSMVLRSASGTMRIVDAYHPIDKLRVKELLPEQVVARPG
                     TA"
     gene            complement(1649..1780)
                     /locus_tag="VIN32_05805"
     CDS             complement(1649..1780)
                     /locus_tag="VIN32_05805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8922128.1"
                     /translation="MRVTAVLERTCVYLDGSGDRRLAKKNDLWVEASRLPIRRRGIS"
     gene            complement(2058..2552)
                     /locus_tag="VIN32_05810"
     CDS             complement(2058..2552)
                     /locus_tag="VIN32_05810"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEY8922129.1"
                     /translation="METVAISGSNGGPDPYGIEPKSTILGRDDALSGKLQLKSGGQIL
                     GNFSGQVESDGDLWIGSEAHVEADIRSDRLTIAGFVRGNIVAASRLKITSTGRLEGDA
                     KVGALVVQEGGVHHGVIRVHPEGVPDGEDKIVVDAAARPAVVAVKNVPNPVGRVRKLW
                     GEFF"
     gene            complement(2645..>3611)
                     /locus_tag="VIN32_05815"
     CDS             complement(2645..>3611)
                     /locus_tag="VIN32_05815"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEY8922130.1"
                     /translation="MDFDGNIQDRLGTARADLEARILRTPADPIQTFSDDPLRMLRAV
                     RFAAQLGFELAPDLLPAMRRLSGRLAPPVVSVERITDELCKMLESERPKVALELLDSG
                     GLLEVVLPEVTACKGVEQGGYHTHDVFGHTLLAVGLTPPDLVVRLAALLHDIGKPVTA
                     TPDGAFTGHEEVGAGLARGALERLRFPQREIDAVTTLVRLHLRPVYYRSEWSDGAVRR
                     LARDAGPLLERLMALARADIAASAYPEPEKLDELAARLAGVLSEQPTRMAAPIDGEDI
                     MRVRGLAPGPEVGRIKDRLGELIMDGEIEPTREAVLAYLQSHSDL"
BASE COUNT          576 a         1263 c         1155 g          617 t
ORIGIN      
        1 tggcgatcgc cagcttccag gccgctgcat aaaggcccga tgggtcgagc gcgtcagtcg
       61 gtggggcggg cacggctcca atcgccccca cggccggcac ggctttcggc ggctctccca
      121 gcaccttggc gacggcggcc acctcagcct ccgcctcccc ggacagcgac ggcagcttct
      181 ggagaaaggc ggcgatctgg cggtcgactg aaggcggagg ctcgtagccc ggccccggca
      241 gcacagtggc gaaaggccgg tagatcgcag cgaactgcaa tggatacagc tgctccctca
      301 ccaggatcgc gagggcgacg acctgtgccg actccagcgc cgcggcggcc gcaggctcag
      361 gcagcgcctt ggcaatcgcc tgcttgatct gagtgaccct gaagccactc gtgaaagcgt
      421 tgaccatgga ctcgtcagca cccgaaagca gcggatccag cagctgctcc atcgcagtat
      481 tggtcggccg ctggcgggaa aggattcgcg ccagcgcgat ccaacgccac tgctcaacgg
      541 tgaaggtctt gccggcggca gccaggaact gcccggcttc ggtggcgctc aggctgtacc
      601 aggacgcgct accacctgct cgggcagcag ctctttgact cgaagcttgt cgatcggatg
      661 gtatgcgtcc acgatgcgca tggttccaga ggcggagcgc aggaccattg actgcgtgta
      721 ggcgtaaacg acgtcgtatc gaacgccttt gagcagctca cctgacgtga ttcctgtggc
      781 ggcgaagaag atgttgttgc ccgaacacag gttgtcgatg gacagcacct tatcgggttg
      841 atgcccctct ttgatcgcga ggtcgcgctc atcgtcgtcg cgtagcagca gcttggcctg
      901 catgtcacca ccgatggcct tgatcgcgca tgcagccagg accccttcgg tggctccgcc
      961 cgaacccatc atgcagtgaa tcccgtaccg catctcgagg gcggcctcga gcgcgccgcc
     1021 gacatcccct gcgggtatca gcttgatgcg cgccccgacg tcccggatct cggcgatgat
     1081 gccatcgttt ctcggccgct cgagcaccac cacggtcagc tcttgcggcg gcaggccctc
     1141 cgccttggcg atcctgatga ggttccattc gactggcatg ttcaggtcga tgacaccctt
     1201 ggcgcggcgg ccgaccacga gcttttgcat gtagtggata tgggtcgtga acatcgtgcc
     1261 gcgctcggcg agggcgatga ccgagatggc tcccggcagg ccggcagcgg tcaggctggt
     1321 cccctcgatc ggatcgaccg ccacatcaag ctcgggttcg aggccgttgc cgatctcttc
     1381 gccgaagtaa agcatcggcg cctcgtcctt ctcaccctct ccgatgacga cggtgccttt
     1441 catgtcgacc gacgcgaggg cgctgcgtat ggcctcgaca gcgcgtccgt cggcgcccat
     1501 cttgtcgccg cgtccttgca gtggagcggc ggccagggcc ccggcctcgg tgaccctgag
     1561 cagctcgagg gctaggttgc ggtccatttc ctgatccatc tagccacgac cccccgtcga
     1621 catagcgccg ggtagcgtat ccgaatccct agctgatgcc gcgccgccgg attggcagcc
     1681 ggctcgcctc cacccagagg tcgttcttct tggccagccg gcgatcgccg ctgccgtcca
     1741 ggtatacgca cgtccgctcg agcactgccg tcacgcgcac ggtgaccccg tccaggatgg
     1801 catcgaaagc gtcgatcagg acctcatcct catttaaagg gacatcgctc aggcgccggg
     1861 atccacgcgg ccgcggcctg gaggaagcca ggggcgcgct cggctgggcc cgcagtctcg
     1921 cgagcgcggg ggatgccttg cgaacgcggg ccgcgccggc tggctcggcc gaacggggac
     1981 cttgggcatc acgcttcgcg cgcataccgc gcgaagcgcc aggttggttg gctcttgacg
     2041 gagtgccgga cgggcctcta gaagaactca ccccacaact tccttacccg gcccaccggg
     2101 ttagggacgt tcttgaccgc gaccaccgcc ggccgcgcgg ccgcgtcgac gacgatcttg
     2161 tcctcaccat cgggcacacc ctccgggtgg actctgatca ccccgtggtg tacgccacct
     2221 tcctgcacca ccagcgcacc gaccttggca tcgccctcga gccgacccgt gctggtgatc
     2281 ttgagacggc tcgcagcgac gatattgccg cgcacgaacc cggcgatcgt caacctgtcg
     2341 ctgcggatgt cggcctcgac atgggcttcg gagccgatcc acaggtctcc atcggactcg
     2401 acctggccgc tgaagttgcc caatatctgg ccgccgctct ttagctggag cttgcctgac
     2461 agcgcgtcgt cgcggcccag aatcgtcgac ttcggctcga tgccataggg gtcggggccc
     2521 ccgttactcc cggaaatcgc gaccgtctcc accccaatgt ccgtcatctg gacctccctt
     2581 acccagcccc ccgctggtac ccattcgcag catagatgct acggagtcag ggtgaatcgt
     2641 aatttcagag gtcggaatgt gactgcaggt acgccagaac cgcctcgcgc gtgggctcga
     2701 tctccccgtc catgatcagc tcgcccaggc ggtcctttat ccggccgacc tcgggccccg
     2761 gcgccaggcc gcggactcgc atgatgtcct cgccgtcgat cggcgcagcc atccgggtcg
     2821 gctgttccga caggacgccc gccaggcgcg ccgccagctc gtcgagcttc tccggttcgg
     2881 gataggcgga cgccgcgata tcggcgcgcg ccagcgccat cagcctttcg agcagagggc
     2941 cggcgtcgcg ggcgagccgc cgaaccgcgc cgtcgctcca ttccgagcgg tagtacacag
     3001 ggcgcaggtg cagccgcacc agggtcgtga cggcgtcgat ctccctctga gggaaccgaa
     3061 gccgctcgag cgcgcctctc gcgaggcccg caccaacctc ctcgtgaccg gtgaaggccc
     3121 cgtccggggt ggcggtgacc ggcttcccga tgtcgtgcag gagcgcggcc agcctcacca
     3181 cgaggtcagg cggcgtgagt ccgaccgcca gcagcgtgtg cccgaagacg tcgtgtgtgt
     3241 ggtacccgcc ctgctcgacc cctttgcagg cggtcacctc gggcagcacg acctcgagca
     3301 gtccacccga gtccagcagc tcgagcgcca ccttcggcct ctcggactcg agcatcttgc
     3361 acagctcatc agtgatgcgc tcgaccgaaa ccactggcgg cgccaggcgg ccgctgagcc
     3421 gccgcatggc cggcagcagg tccggcgcca gctcgaagcc gagctgagcg gcgaaccgca
     3481 ccgctcggag catccggaga gggtcgtcgc tgaaggtctg tatggggtcc gccggagtgc
     3541 ggaggatgcg cgcctcgagg tccgcccgcg ctgtgccgag ccgatcctgg atgttgccgt
     3601 cgaagtccat c
//
LOCUS       DATCQR010000175         3341 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_149683, whole genome shotgun sequence.
ACCESSION   DATCQR010000175 DATCQR010000000
VERSION     DATCQR010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3341
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149683"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(294..1037)
                     /locus_tag="VIN32_05820"
     CDS             complement(294..1037)
                     /locus_tag="VIN32_05820"
                     /inference="COORDINATES: protein motif:HMM:NF024143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEY8922131.1"
                     /translation="MADQVTTTARSMVERLLDPKIQDNVTKRSRALMTAIAEAAQTAS
                     DRAAGGWRDAEPIRRDAARASRDALRWGSRTWRQEMLPSLNQLWNKRTVALGAAGAAV
                     PVARELIDDAAVRMGIRSRHEERHWGSFFVGLLVGAAAGLIAAILTAPKAGREMRDEL
                     AVTAKDAATRAREVASTGAREVATRAREAAESAGDWVPIFQRPETDGELNTGRIAEPT
                     IDTIQTPGVTPPKPARKRAPRPTTVDEVE"
     gene            1260..2372
                     /locus_tag="VIN32_05825"
     CDS             1260..2372
                     /locus_tag="VIN32_05825"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8922132.1"
                     /translation="MTQTTSEAGFLSLGQATSRTIVQRSAARGRLQRTLLVHGPAGSG
                     KGAFVDDLLAMLLCQAVDGERPCNVCSGCRGARARTHPDLVTGSPERWREDRSTGESI
                     VAAARSWLADSAGAPIVGERRVILVEGVDRANEQTQNALLKALEEPTSRHMFVLVADE
                     PTRVLPTIRSRSQSLRIGSVPRADLVAWLVDRERLPADQADALARIAGGMPGTAIGYA
                     RAPRLVDWRRRTQRELLGLLGRGRADRFGSVRDLLDDAARIGVEQPSESEVQVAEEGM
                     RPTGGAQREAALLIVEAWLGLARDLAVAAAGRPQIAAAAALDPELPGIAERIGQPAIR
                     DFIVLLERIRDGLRQNAAPRLALEVAMLAWPTQAPR"
     gene            2369..2671
                     /locus_tag="VIN32_05830"
     CDS             2369..2671
                     /locus_tag="VIN32_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEY8922133.1"
                     /translation="MSGGPAGDVERLTATIIGRVQGVGFRWWVRSAADRMGLTGWVMN
                     GADERSVDVVAEGPAASLDQLAGMLRQGPPGAVVDRVEASRSPASGGYDRFQITRP"
     gene            2668..3015
                     /locus_tag="VIN32_05835"
     CDS             2668..3015
                     /locus_tag="VIN32_05835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8922134.1"
                     /translation="MNWRAVGCGTLAIVVFLAVGLWGMSKAFDRLEGCPDRLQWGDRV
                     YLPTGSHRPQPSFAEGSPVEIGSTFIGLTTRTVWGPPGSASSPSAADRPSRIYLDCDD
                     GSALLFARESLAP"
     gene            complement(3012..>3341)
                     /locus_tag="VIN32_05840"
     CDS             complement(3012..>3341)
                     /locus_tag="VIN32_05840"
                     /inference="COORDINATES: protein motif:HMM:NF024682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HEY8922135.1"
                     /translation="WVGEMIDAVLDNSLRVGEVRMDDETLAVMHELRAFMFERVYLAP
                     SLLREQRAAIDTLSALVDHHLVHPDQIPATYREADAPLVTQVADYVAGMTDRYALAAQ
                     ERIARGR"
BASE COUNT          457 a         1174 c         1209 g          501 t
ORIGIN      
        1 gggccaaagg cgcccaccga tgaagccgca gcgagcgccc cgagcaggac gaggactcgg
       61 cccgccaaga ccgagagtgg cgaggcgggc gacccagccc cgccgaagcc gccaaccagg
      121 tcccgcgcca agcgggccac tgagccggca cctgccgagg gcgcgacgga gggcgccgaa
      181 gagggaatct ggcagcggtt caggggcgct cggaccaagg ctccgtaact ccccggacta
      241 cagagaggcc ggctcgcggg ccggcctctt gcggagatgt tcgaacttgg ctgctactcg
      301 acctcgtcaa cggtggtcgg cctcggagct cgcttgcggg caggcttggg cggcgtgacg
      361 cctggcgtct ggatcgtgtc gatcgtcggc tctgcaatcc gcccagtgtt cagctcgccg
      421 tcagtctccg gtcgctggaa gatcgggacc cagtcgcccg ccgactcggc cgcctcgcgg
      481 gcgcgggtcg ccacctcccg agcgcctgtc gaggccactt cgcgggcgcg ggtcgcggcg
      541 tccttggccg tcactgccag ctcgtcgcgc atctcacgac cggccttcgg cgcggtcagg
      601 atggcggcga tgaggcccgc cgcggcgcca acgagcagcc cgacgaagaa gcttccccag
      661 tgccgctcct cgtggcggct gcgaatgccc atccgaactg cggcatcgtc gatcagctcg
      721 cgcgccacgg gaactgcggc gccggccgca ccgagcgcaa cggtccgctt gttccacagc
      781 tggttgaggc tggggagcat ctcctgcctc cacgtgcggc tcccccagcg cagggcgtcg
      841 cggctcgccc gggcggcatc gcgacggatc ggctcggcgt cgcgccagcc accggcagca
      901 cggtcagacg ccgtctgcgc ggcttcggcg atggcggtca tgagggcgcg gctgcgcttc
      961 gtcacgttgt cctggatctt tgggtcgagc agtcgctcga ccatcgacct ggcagtggtc
     1021 gttacctgat cggccatggc tcacatctcc ttggttctca ggtttggggg cgagccaaag
     1081 tattgcatca ttcccaatct ggcgccccgc cggccgcctt ccggtgcggc ggatccggtt
     1141 gccggccggc cgccagcgcg cgtgcccgag cggttcggcg gggcgacggc atagtggcga
     1201 acagggcctg accggctcct taccgggggc ttatcggctg ccctgcctat actccggcgg
     1261 tgacccagac gacctcggag gcgggctttc tcagcctcgg gcaggcgacc tcccggacga
     1321 tcgtgcagcg ctccgcggcc cgtggtcgcc tgcagcgcac gcttctggtt catggccccg
     1381 ccggcagtgg caagggggcc tttgtcgacg acctgctcgc aatgctcctc tgccaggcgg
     1441 tcgacggcga gcggccctgc aacgtctgtt ccggctgtcg cggggcgcgc gcccgcacgc
     1501 accccgacct ggtgaccgga tcgccggagc gctggcggga ggatcgatcg accggggaga
     1561 gcatcgtcgc tgcggcacgc agctggctcg ccgacagcgc aggtgccccc atcgtcggcg
     1621 agcgccgcgt catcctggtc gagggggtcg atcgggcaaa cgagcagacc cagaatgcgc
     1681 tgctcaaggc actcgaggag ccgaccagcc ggcacatgtt cgtcctggtg gcggacgagc
     1741 cgaccagggt cctgcccacc atccggtccc gaagccagtc actgcggatc gggtcggtgc
     1801 cgcgtgccga tctggtcgca tggctcgtgg atcgagaacg cctgccggcc gaccaggccg
     1861 atgccctggc gcggatcgcg ggcggcatgc ccggcaccgc gatcgggtac gcacgcgcgc
     1921 cgcggctggt ggactggcgt cgccgaacgc agcgcgagct gctggggctg ctgggtcgcg
     1981 gccgggcaga tcggttcggc tcggtccgcg atctcctcga cgacgctgcg cggatcgggg
     2041 tcgagcagcc gagcgagagc gaggtccagg tcgcggagga ggggatgcga ccgaccggtg
     2101 gcgctcagcg cgaggccgcg ctcctcatcg tcgaagcgtg gcttggcctg gcgcgtgatc
     2161 tcgccgtggc ggccgcgggc cgaccgcaga tcgccgcggc cgcggccctc gatccggaac
     2221 tgcccggcat cgcggagcgc atcgggcagc ccgcgatccg cgacttcatc gtgctgctcg
     2281 agcgcatccg cgacggcctg cggcagaacg ccgctccacg cctggccctc gaggtcgcca
     2341 tgctcgcctg gccgacgcag gcgccccggt gagtggcggc cccgcggggg acgtcgagcg
     2401 tctcaccgcg acgatcatcg ggcgagtcca gggggtcgga ttccgttggt gggtccgctc
     2461 ggccgcagat cggatgggcc tgaccggctg ggtcatgaat ggcgccgacg agcggtcggt
     2521 ggacgtggtc gcggagggac ctgcagcctc actcgatcag cttgcaggga tgttgcgcca
     2581 gggccctccc ggggcggtgg tcgaccgcgt tgaggccagc cgatcgccgg cgtccggcgg
     2641 ctacgaccgc tttcagatca cccggccgtg aactggcgcg cagttggctg cgggacgctg
     2701 gccatcgtcg tcttcctggc ggtcgggctg tggggcatgt ccaaggcatt cgaccgtctc
     2761 gagggctgcc ccgaccggct gcaatggggc gaccgcgtgt acctgccgac cggatcgcat
     2821 cggccccagc cgagcttcgc ggagggttcc ccggtcgaga tcggctcaac cttcatcggc
     2881 ctcaccacgc gaacggtatg gggcccgccg ggaagcgcgt cgtcgccatc ggccgctgac
     2941 cggccgagtc gcatctacct cgactgcgac gacggcagcg ccctgctctt cgcaagagag
     3001 agcctcgcgc cctagcggcc ccgcgcgatc cgttcctggg ccgccagcgc atagcggtcg
     3061 gtcatgccgg cgacgtagtc cgcgacctgc gtgacgaggg gagcatcggc ctcgcgatag
     3121 gtggccggga tctgatcggg atggaccagg tggtgatcga ccagtgcgct cagcgtgtcg
     3181 atggcggcgc gctgctcgcg aaggagggat ggcgccaggt agacccgctc gaacatgaac
     3241 gcgcgcagct cgtgcatcac ggccagtgtc tcgtcatcca tgcgcacctc gccgacccgc
     3301 agcgagttgt cgagcaccgc atcgatcatc tccccgaccc a
//
LOCUS       DATCQR010000176         2293 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_41218, whole genome shotgun sequence.
ACCESSION   DATCQR010000176 DATCQR010000000
VERSION     DATCQR010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41218"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2149
                     /locus_tag="VIN32_05845"
     CDS             <1..2149
                     /locus_tag="VIN32_05845"
                     /inference="COORDINATES: protein motif:HMM:NF020083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY8922136.1"
                     /translation="ENAARSLLLPRRRPGQRTPLWQQRMRSASLLKVAGQYPDFPILA
                     ETWRECMADHFDMAALMTLLRGIRSREVRVVAVDTERASPFASSLLFSYVAEFMYAGD
                     SPLAERRAQALTLDRELLAELLGSEDLRELLDPDAIAAVEVELQGLLPERFPRDPDEA
                     HDLLVRLGDLNDTEASARGVTQEWLRTLERERRAVVVRAAGQSRWIAAEDAGRYRGAL
                     GTSLPVGLPEAFLSPGPRPLESLARRWARTHVPFVTSDLATRWGLPVAAVEEALTQLA
                     ARGEIVAGEFRRAAEGREYCHPEVLRMLRRRSLAALRREIESVAPVVLARFLPSWHGI
                     GAKASGVDRLLEVVFQLQGLALPASVIERDVIAGRVANYNPRLLDELVSMGEVVWVGR
                     GALGASDGKVTLYLRGDAPRLVADLADPPAGDLHNRLREHLRARGASFFRDIYGACGG
                     GDEEAMLDALWDLVWSGEVTNDTFAPLRLLGPIARRPARGPRLPRLTQPRASGRWSLV
                     AEMTGAGAAPTERMHALAGVLLQRHGVLTREAVVAEGWPGGFAGLYPVLRAMEESGRI
                     RRGYFVEGLGGSQFALPGAVDRLRSLREPNGAVVALAASDPANAYGAILPWPRSDGRM
                     ARAAGAYCVLDAGELVLYLERGGRSLLTNGEVRPEHVRALMGVAVAAGRVEVQRVDGI
                     GVMESPLAALLRESGFASTHRGLVAYGGRPGRQ"
     gene            2176..2249
                     /locus_tag="VIN32_05850"
     tRNA            2176..2249
                     /locus_tag="VIN32_05850"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2210..2212,aa:Pro,seq:ggg)
BASE COUNT          312 a          750 c          848 g          383 t
ORIGIN      
        1 agagaacgcc gcgcgttctc ttcttctgcc gcgccgccgc cccgggcagc ggacccctct
       61 ctggcagcag cgaatgcgca gcgccagcct cctgaaggtt gccggccagt acccggactt
      121 cccgatcctg gccgagacgt ggcgcgagtg catggccgac cacttcgaca tggccgccct
      181 gatgacgctg ctgcgaggga tccggtcgcg cgaggttcgc gtcgtggccg tcgacaccga
      241 acgggcatcc cccttcgcgt cgtcgctgct gttctcgtac gtcgccgagt tcatgtatgc
      301 gggcgattcg cccctcgccg aacggcgcgc gcaagccctc actctcgatc gggagctgct
      361 ggccgagctg ctcggcagcg aggatctgcg tgagctcctc gaccccgacg cgatcgccgc
      421 agtggaggtt gagctccagg gcctgctgcc cgagcgcttc ccacgcgatc ctgacgaagc
      481 gcacgacctg ctggtccgcc tcggcgacct caacgacacg gaggcttctg ctcgcggcgt
      541 cacgcaggaa tggctgcgga cactggagcg ggagcggcgg gcggtcgtgg tgcgggcggc
      601 gggccagtcg cgctggatcg cggctgaaga tgccggccga tatcgcggag cgctcgggac
      661 gagcctgccc gtgggcttgc ccgaggcgtt tctcagtcca gggccgcgac cgctcgaatc
      721 gctcgcaagg cggtgggcgc gcacccacgt cccattcgtg acctcagacc tcgcgacgcg
      781 gtggggtctg ccggtggcgg cggtcgagga ggcgctgacg cagctggcgg cgcgcggcga
      841 gatagttgcg ggcgagttcc ggcgagcagc cgaaggccgc gagtactgtc accccgaggt
      901 cctgcggatg ctgcggcggc gctcgctggc ggcgctacgt cgtgagatcg agtccgtggc
      961 cccagttgtg ctggcgcgat tcctgccgtc atggcacggc atcggcgcga aggcttcggg
     1021 cgtcgatcgt ctgctcgagg ttgtgttcca gctgcaaggg ctggccctcc cggcatcggt
     1081 catcgaacgc gacgtcatcg caggtcgcgt cgccaactac aacccgcgcc ttcttgacga
     1141 gctcgtttcg atgggagagg tcgtctgggt cggtcgtggc gcgcttggcg ccagcgatgg
     1201 caaggtcacg ctctacctgc gcggcgatgc gccgcgcctg gtggcggatc tggctgaccc
     1261 acctgcgggc gatctgcaca accggctgcg cgagcacctg cgggcgcgcg gcgccagctt
     1321 cttccgagac atctacggcg cctgcggcgg cggcgacgaa gaggcgatgc tcgacgcgtt
     1381 gtgggacctg gtgtggtcgg gagaggtgac caacgacacg tttgcgccgc tgcgcctgct
     1441 cggcccaatc gcccggcggc cggcgcgcgg cccgcggctc ccacggctga cgcaacctcg
     1501 cgcctcaggg cgatggtccc tggtcgcgga gatgacaggg gccggagcgg cgccgacgga
     1561 acggatgcat gcgctggcgg gcgtgctgct gcagcgccac ggtgtgctga cgagggaagc
     1621 ggtcgtcgcc gaaggatggc cgggcggttt cgcgggactt tatccggtgc tgcgcgcaat
     1681 ggaggagtcg ggacggatcc ggcgtggcta cttcgtcgaa ggccttggcg gctcgcagtt
     1741 cgcattgccg ggagcggttg atcgcctgcg ctccctgcgc gaacccaatg gagcagtcgt
     1801 tgcgctcgcg gcgagcgatc ccgcgaacgc gtatggcgcg atcctgccct ggcccagaag
     1861 cgatgggcgc atggcgcgag cggccggcgc atattgcgtc ctcgatgcag gcgagctcgt
     1921 cctctatctg gagcgcggcg ggcgctcgct cctcaccaat ggggaggtca ggccggagca
     1981 cgtgcgagcc ctgatgggcg tcgcggtcgc ggccgggagg gtggaggtgc agcgtgtcga
     2041 tggcatcggc gtcatggaat cgccgcttgc ggcgctgctg cgggagtcag gtttcgcgtc
     2101 gactcaccga ggcctggtgg cgtacggcgg tcggcccgga cgccaatagg gaagccgcta
     2161 cgatagcgag tccatcgggg agtagcgcag ctcggtagcg cacctgactg ggggtcaggt
     2221 ggtcgggggt tcaaatcccc cctccccgac tttctcggac caaagtttcc ttattttgaa
     2281 ctggccctcg agg
//
LOCUS       DATCQR010000177         1720 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_226151, whole genome shotgun sequence.
ACCESSION   DATCQR010000177 DATCQR010000000
VERSION     DATCQR010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1720
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226151"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /locus_tag="VIN32_05855"
     CDS             complement(<1..340)
                     /locus_tag="VIN32_05855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8922137.1"
                     /translation="MQILDAAADQVRQAEDNIIAAFRKSLDEVLRERADPRPLPADDR
                     MIFGLADKAIRDYHAATARSASLPQLSQVQYWEIRQRLYVTHGALGPLGELLAKEDVE
                     DIHIDGLDGGY"
     gene            complement(342..1133)
                     /locus_tag="VIN32_05860"
     CDS             complement(342..1133)
                     /locus_tag="VIN32_05860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8922138.1"
                     /translation="MATIGVFSARGDGATTVAVGLAAVLAATSKTLLIDLNFDNPEIA
                     PLLDVDPEFGVFDLAHNAQLAPVSDADLERRLGRRDGIAVLPGIGREEDVEHISDHFL
                     AGLLQTATRRFDHVVIDLGRLRQGPRASVATGVLLWVATPTPLGMDALERGYWRVEEH
                     GEEWLRRTRLVINRNTGGSLSGVERYAEREYELATAGIIPDAPDYWRRVEFKHSPSAL
                     NIPGTDDPRYRKYHGEQALPVRQALEALAEQVTSAPVELTVSTRT"
     gene            complement(1133..>1720)
                     /locus_tag="VIN32_05865"
     CDS             complement(1133..>1720)
                     /locus_tag="VIN32_05865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8922139.1"
                     /translation="ALVGFGLMVLLGTYAILAFGPGADANQMITVTRAAKAIRAGATI
                     TADELTTGRLRTQDPSLLSTLLLYSDRNRIVGQVADEDIPAGDLVPADVVSAQVTSTL
                     WKLHVAVRGMPADLAPGDHVALLVSETATNGSQVEVVFMQDVRVLSVGQGSVDLWIPA
                     KVVPQVQWFQDHGGIALAAMQPGSGQDKIPAGGGG"
BASE COUNT          271 a          574 c          592 g          283 t
ORIGIN      
        1 aataaccacc gtcgaggcca tcgatgtgga tgtcctcgac gtcttccttg gccagcaact
       61 cgcctagcgg accaagggcg ccgtgggtca cgtacagccg ctggcggatc tcccagtact
      121 gaacctggga gagctgcggc aacgacgcgg atcgcgcggt ggcggcgtgg tagtcgcgga
      181 ttgccttgtc ggcgaggccg aagatcattc ggtcgtcggc cggcagaggc ctcgggtcgg
      241 cccgctcccg cagcacctca tcgaggctct tgcggaatgc cgcgatgatg ttgtcctcgg
      301 cctgccttac ctggtccgcg gcggcgtcga ggatctgcac ctcacgtcct cgtggatact
      361 gtcagctcga ccggcgccga cgtcacctgc tcggccaacg cctccagagc ctgtcgcacc
      421 ggcaacgcct gctcaccgtg gtacttgcgg tagcgcgggt cgtcggtgcc cgggatgttt
      481 agagcggagg gagagtgttt gaactcgacc cggcgccagt agtcaggggc atcggggatg
      541 atgccggcag ttgccagctc gtactcgcgc tcggcgtatc gctcgacgcc agagagggac
      601 ccgccggtat tccgattgat gaccagcctg gtgcgccgaa gccactcctc gccgtgctcc
      661 tcgacccgcc agtagccgcg ctcgagcgcg tccatcccga gtggggtcgg tgtcgcgacc
      721 cacaggagga caccggttgc gaccgaggcg cgaggcccct ggcgcaaccg gccgaggtct
      781 atcaccacgt gatcgaagcg gcgggtcgcg gtctgcagca gccccgccag gaagtgatcg
      841 ctgatgtgtt caacgtcctc ctcgcggccg atacccggca gcaccgcgat gccatcacgc
      901 cttccgagcc gcctctccag gtcggcgtca ctcaccggcg ccagctgcgc gttgtgcgcg
      961 aggtcgaaca cgccgaactc ggggtcgacg tcgagcagcg gggcgatctc ggggttgtcg
     1021 aagttgaggt cgatgagaag cgtcttgctc gtcgccgcga gcaccgcggc cagcccgacc
     1081 gccaccgtgg tcgcgccgtc tccccgcgcc gagaagacgc cgatggtggc catcagccgc
     1141 ctccgccggc cgggatcttg tcttgccctg atcctggctg cattgctgca agggcgatcc
     1201 cgccgtggtc ctggaaccat tgcacctggg gcacgacctt ggccggaatc cagaggtcga
     1261 ccgacccctg gccgaccgag agcactcgca cgtcctgcat gaagacgacc tcgacctgcg
     1321 agccattcgt cgccgtctcg ctgacaagga gcgcgacgtg gtcgccaggc gcaaggtcgg
     1381 ccggcatccc gcggacggcg acgtggagct tccagagcgt ggaggtcacc tgcgccgaga
     1441 cgacgtcggc cggaaccagg tcgccggcgg ggatgtcctc gtccgcgact tggccgacga
     1501 tgcggttgcg gtcggagtag aggagcagcg tggacagcaa cgatgggtcc tgagttcgga
     1561 gccgaccggt ggtcagctcg tcagcggtga tggtcgcgcc cgctcggatg gccttggcgg
     1621 cacgggtgac ggtgatcatc tggttggcgt ctgcgccagg accgaacgcg aggatcgcgt
     1681 atgtgcccaa aaggaccatc agcccgaagc cgaccagggc
//
LOCUS       DATCQR010000178         3061 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_56391, whole genome shotgun sequence.
ACCESSION   DATCQR010000178 DATCQR010000000
VERSION     DATCQR010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3061
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56391"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..910
                     /locus_tag="VIN32_05870"
     CDS             <1..910
                     /locus_tag="VIN32_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HEY8922140.1"
                     /translation="LAFAGSLFGMETKVFMVKVSYEQKPYRRSFIQTFGGTVVPSPST
                     ETNAGRTILAADPDSTGSLGIAISEAVEVAATNDDTKYSLGSVLNHVLLHQTVIGQEA
                     IQQMALAGEEPDVVIGCAGGGSNFSGIAFPFLRDKLAGDSKMRFLAVEPASCPSLTKG
                     EYRYDFGDTVGLTPLVKMHTLGNGFRPAPIHAGGLRYHGMAPMVSHLYELGFIEARAY
                     AQNTCFEAALQFARTEGIIPAPESSHAIRAAIDEALEAKEAGSERVILFNLSGHGYFD
                     MAAYDAYLAGELVDVEYEPQREAVAV"
     gene            990..1274
                     /locus_tag="VIN32_05875"
     CDS             990..1274
                     /locus_tag="VIN32_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002750041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HEY8922141.1"
                     /translation="MPRATWNGTIIAESDTTEIVEGNVYFPPESVKSEFLRPSDTHTT
                     CWWKGEASYYSLEVDGQENPDAAWFYPDASEKAKPIEGYVAFWRGVTVER"
     gene            complement(1271..1933)
                     /locus_tag="VIN32_05880"
     CDS             complement(1271..1933)
                     /locus_tag="VIN32_05880"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006198196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY8922142.1"
                     /translation="MSKGSIGLSDELNAYLIAVGAREPEVLGRLREETAAMPQAGMQI
                     AVEQGALLSMLVKLLNARRILEIGTFTGYSSTAMALALPPDGRIVCCDVSKEFTDVAR
                     RTWAAAGVEDRVDLRLAPALETLEGLLATDGAGSFDLAFIDADKTNYRGYVEGALLLV
                     RQGGLIAIDNVLWSGRIIDPEDTDEDTQAIRDLNADLAMDERVDIAMVPIADGLTLLR
                     VR"
     gene            1975..2847
                     /locus_tag="VIN32_05885"
     CDS             1975..2847
                     /locus_tag="VIN32_05885"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013422032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEY8922143.1"
                     /translation="MKFGLQINSFTWPGGPAAIGPTLARVTRAADDAGFDSIWVMDHF
                     FQIRGLGPPEAPMLEGMTALGFMAANSEKARLGLMVGGIHYRLPGLWIKAVTTLDVLS
                     GGRGWFGIGAAWNEEESTALGFPFPPLGQRFEMLEETLQMAQAMWSGGTGTGEAFHGK
                     HVNATRLLNSPQAISRPRVPIMIGGGGERKTLRLVAQYADACNVFGDASRIAHKYAVL
                     REHCDRLGRPYEEIERSTLQSVDPESQSADQIVAHFGALGEAGAQHILMSVRGVSDVA
                     KLERIGSEVLPQLR"
     gene            complement(2844..>3061)
                     /locus_tag="VIN32_05890"
     CDS             complement(2844..>3061)
                     /locus_tag="VIN32_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014742370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HEY8922144.1"
                     /translation="TDAGVEVLYDDRDESPGVKLTDAELLGMPRIVTISPRSLAAGGA
                     EVTDRASGERSVRPIAEVEAGLLEAVR"
BASE COUNT          499 a         1053 c         1025 g          484 t
ORIGIN      
        1 gctcgccttc gccggcagcc tgttcgggat ggagaccaag gtcttcatgg taaaagtcag
       61 ctacgagcag aagccgtatc ggcgcagctt catccagacc tttggcggca cggtcgttcc
      121 gtcgccctca accgagacga acgccgggcg aaccatcctg gcggccgatc cggactcgac
      181 cggcagcctg ggaatcgcga tcagcgaggc ggtcgaggtg gccgcaacca acgacgacac
      241 caagtactcg ttggggagcg tgctgaacca cgtcctgctg caccagaccg tgatcgggca
      301 ggaggcgatc cagcagatgg ccctggccgg cgaggagccg gacgtcgtga tcggctgcgc
      361 cgggggtggc tccaacttct cggggatcgc cttcccgttc ctgcgcgaca agctggcggg
      421 ggactcgaag atgcgattcc tggccgtcga gcccgcgtca tgcccctccc tgacgaaggg
      481 ggagtatcgg tatgactttg gcgataccgt tggcctgacc ccgctcgtga agatgcacac
      541 gctcgggaac ggcttccgac cggcccccat ccatgccggt gggctgcgct atcacggcat
      601 ggcgccgatg gtgagccatc tatacgagct gggcttcatc gaggccaggg cttatgccca
      661 gaacacctgc ttcgaggctg ctctccagtt cgcccgcacg gaggggatca tcccggcgcc
      721 tgagtcgagt cacgccatcc gggcggcgat cgacgaggcg ctcgaggcga aggaggccgg
      781 cagcgagcgg gtgatcctgt tcaacctctc gggccacggc tacttcgaca tggccgccta
      841 cgacgcgtac ctcgccggcg agctcgtcga cgtggagtac gagccgcagc gagaggcggt
      901 ggcggtctag cccttctccc tctggaccga tggcggcgtc ggactccggc ccggcgccgc
      961 ttttcgcttg cgcggcgtag gatgccgcca tgccacgcgc aacctggaat ggcaccatca
     1021 ttgccgagag cgacaccacc gagatcgtcg aggggaacgt ctacttcccg ccggagagcg
     1081 tgaagtccga gttcctgcgg ccctccgaca cccacaccac ctgctggtgg aagggcgagg
     1141 ccagctacta cagccttgag gtcgacggcc aggagaatcc cgacgccgcc tggttctatc
     1201 ccgatgcctc cgagaaggcc aagccgatcg agggctacgt cgccttctgg cgcggcgtca
     1261 ccgtcgagcg ctagcgaacg cgcaggagcg tcagtccgtc ggcgatcggc accatcgcga
     1321 tgtcgacccg ctcgtccatc gcgaggtcgg cgttcagatc gcggatggcc tgggtgtcct
     1381 cgtcggtgtc ctccggatcg atgatccggc cgctccacaa cacgttgtcg atcgcgatca
     1441 ggccaccctg gcgcacgagg agcagcgcgc cctcgacgta gccccggtaa ttcgtcttgt
     1501 cggcatcgat gaaggccaga tcgaagcttc ccgcgccgtc cgtggccagc agtccctcga
     1561 gcgtctcgag cgccggcgcc agccgcaggt cgacccgatc ctcgaccccc gcggcggccc
     1621 aggtgcgtcg cgcgacgtcg gtgaactcct tcgagacgtc acagcacacg atccggccgt
     1681 cgggcggcag ggcgagcgcc atggcggtcg acgaatagcc cgtgaaggtg ccgatctcga
     1741 ggatccggcg ggcattgagg agcttgacca gcatggagag caaggccccc tgctcgaccg
     1801 cgatctgcat cccggcctgt ggcatcgccg ccgtctcctc acgcaggcga cccagcacct
     1861 ccggctcacg cgccccgacg gcaatcaggt aggcattcag ctcgtcgctc agcccgatcg
     1921 atccctttga catgggtcca tcgtagcgcc cgtagctcag cgtaggatcc gcacgtgaag
     1981 ttcggcctcc agatcaacag cttcacctgg ccaggcggcc ccgccgccat cggtccgacc
     2041 ctggcgcgcg tcacacgcgc cgcggacgac gcggggttcg actcgatctg ggtgatggac
     2101 cacttcttcc agattcgtgg cctgggcccg ccggaggcgc cgatgctcga gggcatgacc
     2161 gcgctcggct tcatggcggc caactcagag aaggcgcgac tgggactgat ggtcggcggc
     2221 attcactacc gcctgccagg cctgtggatc aaggccgtca ccaccctcga cgtgctgtca
     2281 ggcggccggg ggtggttcgg gatcggtgcg gcctggaacg aggaggagtc gaccgcgctc
     2341 gggttcccat tcccccctct cggccagcgc ttcgagatgc tcgaggagac gctgcagatg
     2401 gcgcaggcca tgtggtccgg cgggaccggc accggcgagg ccttccacgg gaagcacgtc
     2461 aacgccaccc ggctcctcaa ctcgccccag gcaatctcgc gcccccgggt gccgatcatg
     2521 atcggcggtg gtggggagcg aaagacgctg cgcctggtgg cgcagtacgc cgatgcgtgc
     2581 aacgtgttcg gggatgcgag ccgcatcgcg cacaagtacg cggtcctgcg cgagcattgc
     2641 gaccgcctgg ggcgcccgta cgaggagatc gagcgaagca ccctgcagag cgtcgatccg
     2701 gaatcgcagt cggcggatca gatcgtcgcc cacttcgggg ctttgggcga ggccggcgcg
     2761 cagcacatcc tgatgagcgt gcgcggcgtg agcgacgtcg cgaagctcga gcggatcggc
     2821 tccgaggtcc tgccgcagct ccgctagcgg acggcctcca ggagccccgc ctccacctcc
     2881 gcgatcgggc gcaccgagcg ctccccgctg gcccgatcgg tcacttccgc gccgcccgcc
     2941 gccagggatc tgggcgagat ggtgacgatg cgcggcatgc cgagcagctc ggcatcggtg
     3001 agcttcactc cgggcgactc gtctcgatcg tcgtagagca cctcgacgcc ggcatcggtc
     3061 a
//
LOCUS       DATCQR010000179        13362 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_226243, whole genome shotgun sequence.
ACCESSION   DATCQR010000179 DATCQR010000000
VERSION     DATCQR010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 13362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13362
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226243"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(82..639)
                     /locus_tag="VIN32_05895"
     CDS             complement(82..639)
                     /locus_tag="VIN32_05895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8922145.1"
                     /translation="MSIRLDPRRLGSLKLLAAEAGIRPGELVTRWVEERLDAARSGAP
                     APSPVDSDAVASLTARVDELARRIDQMAPTPASEPDGEPEAAPVAAPAKRGPGRPRKT
                     GAVARVRKSGPKVALHDEIIAVIRELGPQTAAELASAIVERGRYKAPRSKKPLDAAQV
                     NARVSNPTYRGRFVRREHRIGLATE"
     gene            complement(955..2679)
                     /locus_tag="VIN32_05900"
     CDS             complement(955..2679)
                     /locus_tag="VIN32_05900"
                     /inference="COORDINATES: protein motif:HMM:NF044955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEY8922146.1"
                     /translation="MGVVRFFRVASSVVFFLFWAIVIAVGVFGFPGISSGEHRFPDPV
                     NGQAVYDPAGALDPGIEQALESQVDAIEARSGAELAIYVQVDEFATPESNLAAAQALL
                     DQWGVGRAGFDDGLVILISFEPNLVHGQAHYYAGAGFLRGYLSQGDLQALFDEVILPA
                     AAQGQIGGGLIEAVNLIDAAITPAATARLNFLRQVNGVVGIIGAPLSFLLIVGFAFRA
                     WRREGDDPEVLDSPSILMAGPPTNMTPPLATVVRQGKATQHSINTLLMELASTGRIAF
                     QNLDQAGKVKADDEPNPLLDPAIEIRDDAPTGGRLGSTERDAWDLLRQQALASGVISR
                     ERLWELNALLKAVRGGLEDDVVRLGWFTRPPSQLITRWSFIGIGEMLLGALFVLGGFL
                     IPMSGLTLLGAATGVGGALTWVIGQFMSQRTPNGAYVDAMLKAFRRTLEKTMEQARSM
                     EQVVADPTVKLLANTPDKAVVWGFALGLHDEVAKVLQRTLGDHEKDPSTATGVYYPLW
                     LGSSPASSMQAGMAGGSIFSGSGMPDIGGMFSAVGSIGSSPPSSSSGGGGFGGGGSSG
                     GGGGGGSF"
     gene            complement(2707..3168)
                     /locus_tag="VIN32_05905"
     CDS             complement(2707..3168)
                     /locus_tag="VIN32_05905"
                     /inference="COORDINATES: protein motif:HMM:NF025520.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4307 domain-containing protein"
                     /protein_id="HEY8922147.1"
                     /translation="MTPADPRPLRNCYKCGRQIGPDETICEVCNRAGMATPSATQYHG
                     TIVVAIVAGVALMAIAASLSLRGIGPFHGEAVGWSSDPPDAVVVELRVTNQGTRAGRA
                     KCQVTARDASNLELRIRSIVSPQVQGGATVTFSERIPGLPQAPASLAVTCS"
     gene            complement(3165..3788)
                     /locus_tag="VIN32_05910"
     CDS             complement(3165..3788)
                     /locus_tag="VIN32_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007458046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HEY8922148.1"
                     /translation="MSEHLDLADWRCRVAASYDVLRNDVRAEPARLLGFRAAKDRLFA
                     SHTSSPVPVAARRDFRGLAYWRYAPALAFSAALEPDPDAPPLDVPRSGPGPGMPYRRI
                     GWVSFAVDGTPTRLAVYWLNEYVGGIFIPFRDGTSGTETYGGGRYLWDSGKGADLGSA
                     GDELVIDFNYAYHPSCVYDAVWSCPLAPPENWLTAPIRAGERLTPAS"
     gene            complement(3858..4793)
                     /locus_tag="VIN32_05915"
     CDS             complement(3858..4793)
                     /locus_tag="VIN32_05915"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HEY8922149.1"
                     /translation="MRRYRTLALGAASLVLVLSACTPGGSGGSPSGSAAANKPVVKVG
                     SAGFWEAAVTGEMYAQALEAKGYTVERHLEIGERPAVHSALANGDVNLIPEYLGGLAG
                     QGLGITDLSPDPQKAWDNMQSALADKGWVSFDFSPGTDADGFAVRKETADQYNLKTMS
                     DLAKVAGKLVWGVAEGCPTNPVCGPGLKSTYGIDLAKLQVESLPPCSTDIATALNDSA
                     IGVAQVCTTQPEISSMNFVLLTDDKHLQPAQNLVAIATKELADAAPADFADTLNAVTA
                     KLTTEALTKLGEAIVVNHGEIPDVATQFLKDNGLL"
     gene            complement(4908..5654)
                     /locus_tag="VIN32_05920"
     CDS             complement(4908..5654)
                     /locus_tag="VIN32_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012894560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8922150.1"
                     /translation="MDFLAQVLAWFTDPANWTGNNAIPLRLWQHLSLSVVSLAVGLAI
                     ALPIGLYIGHTGRGARFAVAVSNIGRAVPSLGWLGIAYPISTGLFQRAGIGFLPAFIG
                     LVALAIPPIVTNTYAGLHAVDDELREAARGMGMRELQLLMRVEVPVALTVILAGIRVS
                     AVAVIATAPLAALVGGGTLGAYILQGLALSDEVRVFAAAVMVALLAILTELAFAWLQR
                     RAVSPGLAAPDGGSWVEPSQMPAGRAIAPS"
     gene            complement(5659..6321)
                     /locus_tag="VIN32_05925"
     CDS             complement(5659..6321)
                     /locus_tag="VIN32_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020643021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8922151.1"
                     /translation="MTAGEPIFRLDWVLQHLGMLGQRIGEHLLVTVIAVTVGFVISFA
                     LALAVRRYPRLYSPILAISGVLYAIPSIALFVLFIPITGLSLLTAEIALVSYTLVILV
                     RNIVTGLRGVPPEVIEAARGMGYTDGQRLWRVELPIAMPIIVAGLRIATVTTIGLVTI
                     ATLIGMGGLGYLIVNIGVQRRFPTATITGVVIVVLLSTAVDLGLQLLQRRLTPWARAR
                     EA"
     gene            complement(6318..7457)
                     /locus_tag="VIN32_05930"
     CDS             complement(6318..7457)
                     /locus_tag="VIN32_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8922152.1"
                     /translation="MTSSAAVVTLDHVSKRYGPAGATPAVSDLSLTIPAGEIVVLVGP
                     SGCGKTTTMKMINRLIEPTSGRITIDGEDVMGLPAVELRRRIGYVIQQVGLFPHLTVA
                     ENVAVVPQLLRWPETRVRDRVEELLHLVRLDPSTYRDRYPAALSGGERQRVGVARALA
                     SDPPLMLMDEPFGAVDPILRDRLQNEFLRLQAQVRKTIVFVTHDVDEAIKMADRIAIL
                     QRGGILAQYDTPDAILAEPGGEFVARFVGADRGLKRLSLGRVRDLPLSTPTTARVGEA
                     RADVRQRLDEAGAEYALLLDESDRPVGWIERRELTGEERVTEEDATPGSPTLQPESTL
                     RDALSAMLGSSVQLGVVVDEADRLLGVISVDAISEVLRAPASSAR"
     gene            complement(7454..9520)
                     /locus_tag="VIN32_05935"
     CDS             complement(7454..9520)
                     /locus_tag="VIN32_05935"
                     /inference="COORDINATES: protein motif:HMM:NF021570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2298 domain-containing protein"
                     /protein_id="HEY8922153.1"
                     /translation="MPAEPGGANRLRVVAGIGAALLVLGALVAAWLEPGLAELVVWPL
                     LFVLPGWAIVGWARPRVAATGRLGLAIVLSVALSTHLVYWLSLATGGYRRETIFAVAA
                     MLALPIPIGVMRGGARSVLEQGPAAWHAVRRDWLAFAVAAGCAAFVGAVLQSGLWQAT
                     PNGISAGGSNWSDLGVHLSIAQSLNAGNFPPQVPYFAGEPLVYHWFADFHAAIVARSA
                     GIFAIPSFVVSSAILTGALALLVHGLARAVLRGRGARRAALLAVVLVIFAGGLGWMRL
                     VGDLANGFGDLPNLVTRNSYDNSWLTEWPYFRIPSVMGTGLLVHRATTAGLPMLVGAV
                     LLLVAGLPSARARAAGWRDRPALIFLAGLLGALLAPFHFFFFPAFLALALGWVVVGGR
                     LFDPVAPRNAALLLAPYLLAVPFAIAAVGQAGGSGALHWVAGWESAPTTDGPWAVLFF
                     YLTNLGLPFVLALLALLLPRVPHRGFLAIWLVGLFVVPNVVQVSAIGFDMNKYFQAMW
                     VAVALLAAWMIRRWPTWAVTAVLLLSVPSPLLVSAWTALNREVVLGWSEIEAADWIAA
                     NTPERSVFVTNGGLNSPTDPAGRLRVLTFTPYIANLGYSPDERSAVVDAIYCSGDPQR
                     AAQLTRDLGATYLLDTGPPRACQPTTFEGSPDWELVYDRSGVRIWHVSGGVAAISAPG
                     SVSFGR"
     gene            complement(9510..10076)
                     /locus_tag="VIN32_05940"
     CDS             complement(9510..10076)
                     /locus_tag="VIN32_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8922154.1"
                     /translation="MVIHRPSSPSLRLALPGTGGDRLSGPLGRRLDDRYHYLRLVLAG
                     GKEPIEAVVVGPGGTWALRLRAEPGRYRKRNGHWYRLSAATESWVPWDASPITAARLA
                     GRRLEMYLERATLPSAVTACLVPPRGVEIEWDAAQRPGIQVIRDADALATAIGGEGNL
                     TKAQVDRIVALLDPRQPLPRLTRTARAS"
     gene            10181..10762
                     /gene="folE"
                     /locus_tag="VIN32_05945"
     CDS             10181..10762
                     /gene="folE"
                     /locus_tag="VIN32_05945"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEY8922155.1"
                     /translation="MDERSPGSQISEAVRTLLVELGEDPDRAGLSGTPDRVRRMYAEL
                     TEGYHTDPESVLNSATFEVDYDEMVVVRDIEFFSLCEHHLLPFFGKAHIGYLPRGRII
                     GLSKIPRVVDMYAHRLQVQERLTQQVAGFLMERLNPKGVGCVVEATHLCTVMRGVRKQ
                     GATMVTSSMLGTFRADARTRSEFLTFIGRSSDR"
     gene            10767..11069
                     /locus_tag="VIN32_05950"
     CDS             10767..11069
                     /locus_tag="VIN32_05950"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015889728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HEY8922156.1"
                     /translation="MPYAPLLTEPEIAAALEAIPDWHREEGWLIRTVECPSFRASIAL
                     VSRVADAAEEANHHPDIEINWRHVTFKLTTKASKGLTARDFAMAATIDRLALQVDR"
     gene            11170..11583
                     /locus_tag="VIN32_05955"
     CDS             11170..11583
                     /locus_tag="VIN32_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003867699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HEY8922157.1"
                     /translation="MDDSRSTPIRVLIAKPGLDGHDRGAKVLARGLRDEGFEVIYTGL
                     RQTPEMIVDAAEQEDVDVIGMSILSGAHMALLPRVVELSRGRGMDDVLIAAGGIIPDA
                     DVAKLKAAGIAEIFGPGTSIGQIAGYFREHARRRA"
     gene            11589..12521
                     /gene="meaB"
                     /locus_tag="VIN32_05960"
     CDS             11589..12521
                     /gene="meaB"
                     /locus_tag="VIN32_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HEY8922158.1"
                     /translation="MAGLPGLLSGAVAGERLALARLLTAIENDAPGLRELLPQLFAAG
                     RGGHLVGITGPPGSGKSTLVNALTEEWRRRGRRVGIVAVDPSSPYTGGAIMGDRIRMM
                     GHAADREVFMRSMASRGELGGIAAATWMAAAALDAAGYDPILIETVGAGQSEVEIARL
                     AETTVVVEVPEMGDEVQAIKAGLLEVADLLVVNKGDRPGADLAARHLRAMLSTAGGRV
                     GRKPPPIMLTTATSGEGVAALADAIEAHRSTARSPEQATARARNQVRRALADLATSRG
                     SRHADWEATVASVANRELDPITAAERLLGEEREA"
     gene            complement(12518..13003)
                     /locus_tag="VIN32_05965"
     CDS             complement(12518..13003)
                     /locus_tag="VIN32_05965"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8922159.1"
                     /translation="MTPAEPIVRLRPMTAEEFPAFVAESKAGYAHDIEVHGGQTREAA
                     LAKAEADFPAILPLGLETPSHAIFVIEATGAAVGRLWLADRELGGRRTLFIYDISIDP
                     GQQGRGYGRAAMGLAEEEARSRGIGRIELNVFGGNDVARNLYRSLGYVETSVQMGKDL
                     G"
     gene            13042..>13362
                     /locus_tag="VIN32_05970"
     CDS             13042..>13362
                     /locus_tag="VIN32_05970"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEY8922160.1"
                     /translation="MSLNRVFVIGAGLMGHGIAQVSAAAGKRVTLADRTAELAEKGKA
                     RIAGNLARQVEKDKLSQEDADALLSRIATAEGATGAGGHEILIEAVFEDESVKRETWQ
                     ALSSA"
BASE COUNT         2140 a         4654 c         4712 g         1856 t
ORIGIN      
        1 acacgggcag cgagggcgcg gtggagttcc aggccaacat cctggtcacc ttcatcctcg
       61 agggatgagg cggccggagc cctactctgt ggcgagcccg atgcggtgct cgcgacgcac
      121 gaagcggccg cgataggtcg ggttcgagac ccgcgcgtta acctgcgctg cgtcaagcgg
      181 cttcttgctt cttggcgcct tgtaacggcc gcgctcgacg atcgctgacg ccagctccgc
      241 tgctgtctgc ggtccaagct ccctgatcac cgcgatgatc tcgtcatgca gcgcgacctt
      301 cgggccggac ttcctgacgc gggcaacggc accagtcttc cgtggccgcc ccggtccgcg
      361 cttcgcaggc gctgcgacgg gtgccgcctc gggctcccca tccggctccg aggccggcgt
      421 tggagccatc tgatcgatgc gccgggcgag ctcatccacg cgtgcagtga gcgatgccac
      481 agcgtccgag tcgaccggcg atggtgccgg cgcgccggag cgggcggcat ccaggcgctc
      541 ctcgacccag cgtgtcacca gctcgccagg gcggattccc gcctcggcgg cgagcagctt
      601 cagcgagcca aggcgccgcg ggtcgaggcg gatcgacatg cccccgtcat ccatcggtcg
      661 tggcgcaaag ccggtagggc gaggcggtcg gtcgccgaag ctgcgctccc ggggcgggcc
      721 ggagaagtcg ccgccttctc ggggcggacg gtcggtgtag gcggggcggt cccgctgggc
      781 gcggtcgcca taggctgggc gatcccgctg cgggcggtcg ccgaagccgg ggcggtcgcc
      841 agggggtcgt ccggagccgc cgggtcggcc gccgccgggg cgtcggtcag gtcgtcgatc
      901 gtatggcatc gggctcacca tagccgctgg acagccgggg cgccagcagc gggactagaa
      961 gctgccaccg ccgccaccgc cgccggagct gccaccgcca ccgaagccgc ccccgccgga
     1021 ggacgaagag ggaggcgatg acccgatact gccgacggcg ctgaacatgc caccgatgtc
     1081 cggcatgccg gagcccgaga agatgctccc cccggccatc ccggcctgca tggatgacgc
     1141 aggagagctg ccgagccaga gtgggtagta gacgccggtg gccgtcgacg gatccttctc
     1201 gtggtccccg agcgtgcgct gcaggacctt ggcgacctcg tcgtgcaggc cgagggcgaa
     1261 gccccacacc accgccttgt cgggagtgtt ggcgaggagc ttgacggttg ggtcggcgac
     1321 cacctgctcc atgctgcgcg cctgctccat ggtcttctcg agggtgcgcc gaaacgcctt
     1381 cagcatggcg tcgacgtagg cgccgttggg agtccgctgt gacatgaact gcccgatcac
     1441 ccaggtcagc gccccaccga cccccgtcgc tgcgccgagg agcgtcagac ctgacatcgg
     1501 gatcaggaag ccgcccagca cgaagagcgc gccgagcagc atctcaccga tcccgatgaa
     1561 gctccagcgc gtgatcagct gtgatggtgg acgggtgaac cagcccaggc gcaccacgtc
     1621 gtcctcgagt ccgccgcgca ccgccttgag cagggcgttg agctcccaga gacgctcgcg
     1681 ggagatgacg ccgctcgcaa gcgcctgctg gcgaaggagg tcccacgcat ctcgctcggt
     1741 gcttcccagg cgacccccgg tcggcgcgtc atcgcggatc tcgatggccg ggtcgaggag
     1801 cgggttcggc tcgtcatcgg ccttgacctt gcccgcctga tccaggttct ggaacgcaat
     1861 ccggccggtg ctggccagct ccatcagcag ggtgttgatc gagtgctggg tcgcctttcc
     1921 ctgacggacc accgtggcga ggggcggcgt catgttggtc ggcggacccg ccatcaggat
     1981 cgaaggcgag tcgaggacct cgggatcgtc cccctcgcgc cgccaggcgc ggaatgcgaa
     2041 accgacgatc agcaggaagc tgagcggtgc cccgatgatg cccaccaccc cgttgacctg
     2101 gcgcaggaag ttgagccgcg cggtcgccgc cggagtgatc gccgcatcga tcaggttgac
     2161 cgcctcgatg aggccgccac cgatctgccc ctgcgccgcg gcagggagga tcacttcgtc
     2221 gaagagcgcc tggagatcgc cctgcgagag atagccgcgc aggaagccgg cgccggcgta
     2281 gtagtgggcc tgcccatgga ccaggttcgg ttcgaagctg atgaggatga ccagcccgtc
     2341 gtcgaagcct gcccggccga ccccccattg atccaggagc gcctgggcgg ccgccaggtt
     2401 cgattctggc gtggcgaact cgtcgacctg cacgtagatg gccagctcgg cgccgctgcg
     2461 cgcctcgatc gcatcgacct gtgactccag cgcctgctcg atccccgggt ccagcgcccc
     2521 ggcggggtca tagaccgcct gcccgttgac ggggtccggg aaccggtgtt cgccggagga
     2581 gatcccggga aagccgaata ccccaacggc aatcacgatc gcccagaaca ggaagaagac
     2641 gacgctgctg gcgacccgga agaagcggac cacacccacg cgggcaggat agacgcccgg
     2701 cagcgactag gagcaggtca ccgccaggct cgccggcgcc tgcggcagac cggggatgcg
     2761 ctctgagaag gtgaccgtgg caccgccctg cacctgcggc gatacgatgg agcggatgcg
     2821 cagctccagg ttggatgcgt cgcgcgccgt gacctggcat ttcgcccgac cggcacgcgt
     2881 cccctggttg gtcacccgca gctccaccac cacggcgtcg ggcgggtcgc tgctccagcc
     2941 cacagcctcg ccgtggaagg gcccgatccc gcgcaacgag aggctggcgg cgattgccat
     3001 gagcgcaacc ccggccacga tggcgaccac gatcgtgccg tggtactgcg tggccgaggg
     3061 ggtggccatg ccggcccggt tgcagacctc gcaaatcgtc tcgtccggcc cgatctggcg
     3121 tccgcacttg tagcagttgc gcagggggcg gggatcggct ggcgtcacga cgcgggggtc
     3181 aagcgctcgc cagcgcggat cggggccgtg agccagttct cgggcggtgc cagcggacag
     3241 ctccagaccg cgtcgtagac gcacgagggg tggtaggcgt agttgaagtc gatcaccagc
     3301 tcatccccgg cggagccgag gtccgcgccc ttgccggagt cccacaggta gcggccgcca
     3361 ccgtacgtct cggtgccgct ggtgccgtcg cggaacggga tgaagatccc gccgacgtac
     3421 tcgttcagcc agtagaccgc gagccgcgtc ggcgtcccgt cgaccgcgaa gctgacccag
     3481 ccgatcctcc tgtagggcat gcccggtccg ggtccggagc gggggacgtc gagcggcggc
     3541 gcgtccgggt cgggctcgag ggctgcgctg aaggcgagcg cgggagcgta ccgccagtag
     3601 gccagccccc gaaagtcacg ccgcgccgcg acgggcaccg gcgaggaggt atgagaggca
     3661 aagaggcggt ccttggccgc gcggaacccc agcagccgcg ccggctcggc gcgcacgtcg
     3721 ttgcgcagga cgtcatagga cgccgccacg cggcaacgcc aatcggcaag gtcaaggtgc
     3781 tcggacatcg gtccggaaag tgtacgggcg gcgccgcccg taggctgcgc cgcccgcatc
     3841 agtctcaggg gagggcctta cagcaggccg ttgtccttga ggaattgcgt ggccacgtcg
     3901 gggatctcgc catggttcac cacgatcgcc tcaccgagct tggtgagggc ctcggtcgtg
     3961 agcttggcag taacggcatt cagcgtgtcg gcgaagtccg ccggagccgc atcggccagc
     4021 tccttggtgg caatcgcaac caggttctgg gccggctgca ggtgcttgtc gtccgtgagc
     4081 agcacgaagt tcatgctcga gatctcgggc tgggtggtgc agacctgggc gacaccgatt
     4141 gcgctgtcgt tcagcgcggt ggcgatgtcg gtgctgcatg gcggcaggct ctccacctgc
     4201 agcttggcga ggtcgatccc gtaggtggac ttcaggcccg ggccacagac cgggttggtg
     4261 gggcagccct cagccacccc ccagaccagc ttgccggcga ctttggcaag gtcgctcatg
     4321 gtcttcaggt tgtactggtc agccgtctcc ttgcggacgg caaagccgtc ggcatcggtg
     4381 cccggagaga aatcgaatga gacccagccc ttgtcggcga gcgccgactg catgttgtcc
     4441 cacgccttct gcgggtcggg cgagagatcg gtgataccga gcccctggcc cgcgaggccg
     4501 ccgaggtact cggggatcag gttgacgtcg ccgttcgcca gtgcgctgtg caccgcgggc
     4561 cgctcaccga tctcgaggtg gcgctcgacg gtgtagccct tggcctccag ggcctgggca
     4621 tacatctcgc cggtaacagc cgcctcccag aagccggcgg atccaacctt caccactggc
     4681 ttgttggctg ccgcgcttcc gcttggcgag cccccgctcc cacccggcgt gcaggcgctc
     4741 aggacgagca ccagcgacgc cgccccgaga gcgagcgtgc ggtatctccg cattgggtcc
     4801 tccttgttga atgggactgt gcgcgccgcg ctcctgcgcg accgttcgag tctagcaaag
     4861 ctccctcgac accaccgatc cagcgtttgg tggtgtcgca aagccgatca ggacggtgcg
     4921 atcgcccgcc cggctggcat ctgcgatggc tcgacccagc tcccgccatc aggggccgcc
     4981 agcccgggcg agactgcccg gcgctggagc caggcgaatg ccagctcggt caggatcgcc
     5041 agcagcgcga ccatgaccgc ggccgcgaag acacggacct cgtcgctcag cgccagcccc
     5101 tgcaggatgt acgcgcccag cgtccccccg ccgaccagcg ccgccagcgg ggccgtggcg
     5161 atgaccgcga ccgccgagac acggatccct gccaggatca ccgtcagcgc caccggcacc
     5221 tccacgcgca tcagcagctg gagctcccgc atgcccatcc cgcgcgccgc ttcgcgcagc
     5281 tcgtcgtcca ccgcatgcag gcccgcgtag gtgttcgtga cgatcggcgg gatggccagg
     5341 gcgacgaggc cgatgaaggc tggcaggaac ccgatccccg ctcgctggaa gagcccggtg
     5401 ctgattgggt atgcgatccc cagccagccg agcgatggca ccgcccgccc gatgttgctg
     5461 acggcgaccg cgaaccgtgc gccccgcccg gtgtgcccga tgtagaggcc gatcggcagc
     5521 gcaatggcga gccccacggc caaggacacg acagagaggg aaaggtgctg ccagagccgc
     5581 agcgggatcg cgttgttgcc ggtccagttg gcggggtcgg tgaaccaggc caggacctgg
     5641 gccaggaagt ccatcggctc aggcctcccg cgcccgtgcc cagggcgtca gccggcgctg
     5701 gagcagctgc aaccccaggt cgaccgcggt cgagaggagc accacgatca cgacgccggt
     5761 gatcgtcgcc gtcggaaagc gacgctgcac cccgatgttc acgatcaggt agcccagccc
     5821 gcccatcccg atgagggttg cgattgttac gaggccgatg gtggtcaccg tggcgatccg
     5881 caggccggcc acgatgatcg gcatcgcgat cggcagctcg acgcgccaga gtcgctgccc
     5941 atcggtgtag cccattccgc gcgctgcctc gatcacctcg ggcgggacgc cgcgcaaccc
     6001 ggtgacgatg ttgcgcacca ggatcaccag cgtgtagctg acgagggcga tctcggcggt
     6061 gagcagggag agtccggtaa tcgggatgaa gagcacgaac aacgcgatgc tggggatcgc
     6121 gtacaggacc ccgctgatgg cgaggatcgg gctgtagagg cgggggtagc ggcgcaccgc
     6181 cagggcgagg gcgaaggaga tcacgaagcc gactgttacg gcgatcacgg tcaccagcag
     6241 gtgctcgccg atgcgctgcc cgagcatgcc aaggtgctgc agcacccagt cgaggcggaa
     6301 gatcggctca ccggcggtca tcgcgcgctc gaggccgggg cacgcaggac ctcggagatg
     6361 gcgtcaacgc tgatcacgcc gagcagccga tcagcctcgt ccaccacgac tccgagctgt
     6421 accgatgaac cgagcatggc cgatagcgcg tcgcgcagcg tgctctcggg ctggagcgtc
     6481 ggggagccgg gcgttgcgtc ctcctccgtc acccgctcct cgccggtcag ctcacgacgc
     6541 tcgatccagc cgaccggccg atccgactcg tcgagcagca gcgcgtactc ggcgcccgcc
     6601 tcgtcgaggc gctgacgcac gtcagcacgg gcctctccga ctcgcgcggt ggtcggcgtg
     6661 ctcagcggca ggtcgcggac ccgtcccagc gagagccgct tcaggcctcg atcggccccg
     6721 acgaagcgag cgacgaattc gcccccgggc tcggccagga tggcgtcggg agtgtcgtac
     6781 tgggcgagga tcccgccacg ctgcaggatc gcgatccggt ccgccatctt gatcgcctcg
     6841 tcgacgtcgt gggtgacgaa gacgatcgtc ttgcgcacct gcgcctgcag tcgcaggaac
     6901 tcgttctgca gccggtctcg caggatcggg tccaccgcgc cgaacggctc gtccatcagc
     6961 atcagcggcg gatcggaggc cagggcacgc gccaccccca cccgctgacg ctcaccgccc
     7021 gagagggcgg cggggtaccg atcgcggtag gtcgagggat cgagccgtac gaggtggagc
     7081 agctcctcga cccgatcgcg gacgcgggtc tccggccatc gcagcagctg cggcaccacc
     7141 gccacgttct cggccacggt gaggtgcggg aagagcccga cctgctggat cacgtagccg
     7201 atccgccgcc gcagctcgac cgcaggtaga cccatcacgt cctcgccatc gatcgtgatc
     7261 cgacccgagg tcggctcgat cagccggttg atcatcttca tggtggtggt cttgccgcag
     7321 cctgacgggc cgaccagcac gacgatttcg ccggcgggga tcgtcagcga caggtcggac
     7381 accgccggcg tggcacccgc ggggccgtac cgcttgctga cgtgatcgag ggttacgacc
     7441 gcggcgctgc tggtcaccgt ccgaatgata cggagcccgg cgcgctgatc gcggccaccc
     7501 cgccggagac atgccagatc cgcacgccgg atcggtcgta gacgagctcc cagtccgggc
     7561 ttccctcgaa ggtcgtcggc tggcaggcac gcggaggacc cgtgtccagc agataggtgg
     7621 cgccgaggtc gcgggtcagc tgagcggccc gctgggggtc tcccgagcag tagatcgcgt
     7681 cgaccaccgc tgaccgctca tcgggcgagt agccgaggtt ggcgatgtac ggggtgaagg
     7741 tcagcacccg caggcgaccg gccgggtcgg tcggcgagtt gagcccgcca ttggtcacga
     7801 acaccgagcg ctccggggtg ttggcggcga tccagtccgc ggcctcgatc tcgctccagc
     7861 ccagcaccac ctcgcggttc agcgcggtcc aggccgagac gaggagcggc gacgggacgc
     7921 tcagcagcag gacggcggtc accgcccagg tcggccagcg ccggatcatc caggcggcaa
     7981 ggagcgccac cgcgacccac atcgcctgga agtacttgtt catatcgaag ccgatggcgc
     8041 tgacctggac gacgttgggc accacgaaga ggcccaccag ccagatcgcc aggaagccac
     8101 gatgggggac ccgcggcagg aggagggcca gcagcgccag cacaaagggc agccccaggt
     8161 tcgtcaggta gaagaagagg accgcccacg gcccatcggt cgtcggcgcc gattcccagc
     8221 cggcgaccca gtggagcgca ccactcccgc ctgcctgccc cacggccgcg atcgcgaacg
     8281 gcacggccag caggtacggc gccagcagca gggccgcatt gcgcggtgcc actggatcga
     8341 agaggcggcc gccgacgacg acccagccca gcgccagggc caggaacgcg gggaagaaga
     8401 agaagtggaa gggcgccagc agggcgccca gcaggccggc caggaagatg agcgccggtc
     8461 ggtcacgcca gcccgccgcc cgggcccggg ccgatggcag gcccgcgacc agcaggagga
     8521 cggccccgac gagcatcggc agccccgccg tcgttgcacg gtgcacgagg agccccgttc
     8581 ccatcacgga tgggatgcgg aagtacggcc actcggtcag ccacgagttg tcgtacgagt
     8641 tgcgcgtgac caggttgggc aggtcaccga atccgttggc gaggtccccg accagccgca
     8701 tccatcccag tccgccggcg aagatgacca gcaccaccgc cagcaacgcg gctcgtcgag
     8761 cgccgcggcc ccgcaggacg gcgcgcgcca gcccgtggac gaggagcgcc agcgccccgg
     8821 tgaggatcgc ggacgacacc acgaagctgg ggatggcaaa gatcccggcc gagcgggcga
     8881 cgatcgcggc gtggaaatcc gcgaaccagt ggtagacgag cggctcgccc gcgaagtacg
     8941 gcacctgggg cggaaagttg cccgcgttga gggactgggc gatcgacagg tggacgccga
     9001 ggtcgctcca gttcgagcct ccggccgaga tcccgttcgg ggtcgcctgc cagaggccgg
     9061 actgcaggac cgctcccacg aacgccgcgc agccagcggc cacggcaaac gccagccagt
     9121 cgcggcggac ggcatgccac gccgcgggcc cctgttcgag aacggagcgc gcgccgccgc
     9181 gcatgacgcc gatcgggatc gggagtgcca gcatcgctgc cacggcgaag atggtctcgc
     9241 gccggtagcc gccggtcgcg agcgagagcc agtagaccag gtgcgtgctg agcgccaccg
     9301 acagcacgat cgccagcccg aggcgtcccg ttgccgcgac acgcggccgt gcccagccaa
     9361 cgatcgccca cccgggcagc acgaagagga gcggccagac caccagctcg gccaggcctg
     9421 gctccagcca cgccgccacc agggcgccca ggaccaggag ggcggccccg atcccggcga
     9481 ctaccctcag ccggttggcg cctcccggct cagctggcac gcgcggtccg cgtcaggcgg
     9541 ggcagcggtt ggcgcgggtc cagcagcgcg acgatccgat ccacctgggc cttggtcagg
     9601 ttgccctcgc caccgatcgc cgtcgcgagc gcatccgcat cccggatgac ctggatgccg
     9661 ggccgctggg cggcgtccca ctcgatctcc acgccccgcg gcggcaccag gcaggcggtg
     9721 acggctgaag gcagcgtcgc ccgctcgagg tacatctcca gtcgacggcc ggccagccgc
     9781 gcggcagtta tggggctggc atcccagggg acccaggatt cggtcgcggc gctcagccga
     9841 taccagtggc cgttccgctt tcggtagcgg cccggctcgg cgcggagcct cagcgcccag
     9901 gttccgcccg gcccgaccac gaccgcctcg atcggttcct tgccgcccgc cagcacgagc
     9961 cgcaggtagt ggtagcggtc gtccagcctt cgccccagcg gcccgctcag ccgatcaccg
    10021 ccggtccctg gcagggcgag gcgcagcgac ggcgaggagg gtcggtgaat caccatggcg
    10081 acggggacgg tccgtgcgga ttcggcgggg cgcgtctcga gtctaccgcc gccgatgcgc
    10141 ctcgcctagc ccgcgcgggt gccctgctag catgccggtc atggacgagc gctcaccggg
    10201 cagccagatc tccgaggcgg tcaggaccct cctcgtggag cttggagagg acccggaccg
    10261 cgccggcctg tccggcaccc cggaccgcgt ccggcgcatg tacgccgagc tgaccgaggg
    10321 ctaccacacc gatcccgaat cggtcctgaa cagcgccacc ttcgaggtcg actacgacga
    10381 gatggtggtg gtccgtgaca tcgagttctt ctcgctgtgc gagcaccatc tgctcccctt
    10441 cttcggtaag gcgcatatcg gctacctgcc gcgcggtcgg atcatcggcc tgtccaagat
    10501 tccgcgcgtc gtggacatgt acgcgcaccg cctgcaggtg caggagcgcc tgacgcagca
    10561 ggtggctggc ttcctgatgg agcggctgaa cccgaagggg gtcggctgcg tggtcgaggc
    10621 gacgcacctg tgcacggtca tgcgcggcgt gcgaaagcag ggggcaacca tggtcaccag
    10681 ctccatgctc ggcaccttcc gtgccgatgc ccgcacccga tccgagttcc tgaccttcat
    10741 cggtcgctcg tcagaccgct agcccgatgc cgtacgcccc gctcctgacc gagccggaga
    10801 tcgccgcagc gctcgaggcc attcccgatt ggcatcgcga ggaaggttgg ctgatccgga
    10861 ccgtcgaatg cccgtcattc cgcgcctcga tcgcccttgt cagcagggtc gccgacgccg
    10921 cggaggaggc caaccaccat cccgacatcg agatcaactg gcggcacgtg acgttcaagc
    10981 tgaccaccaa ggcgtcgaag ggtctgacag ccagggactt cgcaatggcc gcgacaatcg
    11041 accgcctggc cctacaggtg gaccgatgaa gatcggcctg atgctgccgc tgcccgcgca
    11101 acgccgcggc ggtcaccgag ctcggccgcg cagccggggt cgcgcaggcg gacgctagaa
    11161 tcggcgcgca tggatgactc ccgcagcaca ccgatccgcg tcctgatcgc caagccgggg
    11221 ctcgacggcc acgaccgcgg cgccaaggtg ctggcccgcg ggctgcgcga cgaaggcttc
    11281 gaggtcatct acaccggcct gcgccaaacg ccggagatga tcgtcgatgc cgcggagcag
    11341 gaggacgtcg acgtgatcgg catgtcgatc ctgtccgggg cgcacatggc actcctgccc
    11401 cgcgtggtcg agctgagtcg gggccgtggc atggacgacg tgctgatcgc agccggcggg
    11461 atcatccccg atgccgacgt cgcgaagctg aaggcggcgg ggatcgccga gatctttggc
    11521 cctggcacca gcatcgggca gatcgcgggc tacttccgcg agcacgcccg ccggcgcgcc
    11581 tgagggtcgt ggccggtctc ccaggactgc tgagcggcgc cgtggcgggt gagcggctgg
    11641 cccttgctcg tctgctgact gcgatcgaga acgacgctcc cggcctgcgc gagctgctgc
    11701 cccaactctt cgccgcgggg cgtggtgggc acctggtcgg gatcaccgga ccgccgggca
    11761 gcggcaagtc aaccctcgtc aacgccctga ccgaggagtg gcgccgccgc ggccggcgag
    11821 tggggatcgt ggcggtggat ccctcttcgc cctacaccgg cggcgcgatc atgggcgacc
    11881 ggatccggat gatgggccat gcggcggacc gcgaggtctt tatgcgctcg atggccagcc
    11941 ggggcgagct gggcggcatc gccgctgcga cctggatggc tgctgcggcc ctcgacgccg
    12001 ccggctacga cccgatcctg atcgaaacgg tcggcgcggg ccagagcgag gtcgagatcg
    12061 cccgcctggc cgagacgacg gtcgtagtcg aggttcccga gatgggggac gaggtgcagg
    12121 cgatcaaggc ggggctgctc gaggtcgccg acctgctcgt cgtcaacaag ggcgatcgtc
    12181 cgggcgccga tctggccgcc cgccacctgc gggcgatgct gtccactgcg ggcggtcgtg
    12241 tcggccgcaa gcccccgccg atcatgctga ccaccgcgac cagcggcgag ggggttgccg
    12301 cgctggcgga tgcgatcgag gcccaccggt ccacggcccg ctcgccggag caggcgaccg
    12361 cccgcgcacg gaaccaggtg cgccgcgcac tggccgacct ggcgacgagc cgcgggtcac
    12421 gccacgccga ctgggaggcg accgtggcat cggttgcgaa ccgcgagctc gacccgatca
    12481 ccgccgcgga gcggctgctg ggcgaggagc gagaggccta gcccaggtcc tttcccatct
    12541 ggaccgatgt ctccacgtag ccgagcgagc ggtacaggtt gcgggcgacg tcgttgccgc
    12601 caaagacatt gagctcgatt cgcccgatgc cccggctccg cgcctcctcc tccgccagcc
    12661 ccatcgcagc acgaccgtag ccgcgcccct gctggcccgg atcgatcgag atgtcgtaga
    12721 tgaacagggt tcgtcgtccg cccagctcgc gatcggccag ccagagcctt ccgacggccg
    12781 ctcctgtggc ctcgatcacg aagatcgcgt ggctgggcgt ctccagcccg agcggcagga
    12841 tcgccgggaa gtcggcctca gccttcgcca gcgcagcctc gcgggtctgg ccgccatgca
    12901 cctcgatgtc gtgggcgtag ccagccttgg actcggccac gaaggcggga aactcctctg
    12961 ccgtcatcgg tcgcagacgg acgattggct cggcgggtgt cacggtcggg gggattatcg
    13021 cggctggtac gctactcagc catgagcctc aaccgggtat tcgtcatcgg cgccggcctc
    13081 atgggacacg gcatcgccca ggtgagcgcg gcagcgggca agcgggtgac cctcgcagat
    13141 cggaccgccg agctggccga gaaggggaag gcacggatcg cgggcaacct cgcccgccag
    13201 gtcgagaagg acaagctatc ccaggaggat gcggacgcgc tcctgtcgcg catcgccacg
    13261 gccgagggtg caacgggggc tggcggacac gagatcctga tcgaggccgt cttcgaggac
    13321 gaatcggtca agcgggagac atggcaggcg ctcagctcgg cc
//
LOCUS       DATCQR010000180        17101 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_25599, whole genome shotgun sequence.
ACCESSION   DATCQR010000180 DATCQR010000000
VERSION     DATCQR010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 17101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17101
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25599"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            7..405
                     /gene="rpsH"
                     /locus_tag="VIN32_05975"
     CDS             7..405
                     /gene="rpsH"
                     /locus_tag="VIN32_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEY8922161.1"
                     /translation="MNVTDPIADMLTRIRNASLARHRELTLPSSRIKRDIARILVEEG
                     FIESYATSQDGVQELVTIQLKYVEGKTPVVSGLKRISKPGLRVYARKTEIPRVLGGLG
                     TAILSTSHGIMTGQSARKLNLGGEVLCYVW"
     gene            418..969
                     /gene="rplF"
                     /locus_tag="VIN32_05980"
     CDS             418..969
                     /gene="rplF"
                     /locus_tag="VIN32_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEY8922162.1"
                     /translation="MSRIGRMPIPLPSGVEVTQQGATLSVKGPLGTLERTIHPEMAVE
                     REEGELRVVRPSDEPRHRALHGLTRTLVNNMVVGVTTGFTKGLEISGVGYRAQLQGTK
                     LVLALGYSHPVEVDPPEGISFQLETPTRLAVVGADKELVGQVAAYIRSRRKPEPYKGK
                     GIRYAGEQILRKAGKAGKVGTGK"
     gene            969..1328
                     /gene="rplR"
                     /locus_tag="VIN32_05985"
     CDS             969..1328
                     /gene="rplR"
                     /locus_tag="VIN32_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011064538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEY8922163.1"
                     /translation="MIKKLSRDRLRRKRHERIRLRITGSSARPRLSIFRSAKFIYAQV
                     IDDSSGRTLASASSREATLAAGPGKVESAKAVGLALAQRAKAAGVGAVVLDRGGYRYH
                     GRVRSLTEGAREGGLDL"
     gene            1339..1836
                     /gene="rpsE"
                     /locus_tag="VIN32_05990"
                     /pseudo
     CDS             1339..1836
                     /gene="rpsE"
                     /locus_tag="VIN32_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018214399.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
     gene            1949..2152
                     /gene="rpmD"
                     /locus_tag="VIN32_05995"
     CDS             1949..2152
                     /gene="rpmD"
                     /locus_tag="VIN32_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEY8922164.1"
                     /translation="MLRVTWVKSTIGHKAGARGTIRALGLHRLHETVEVTDTPVIRGM
                     LRRVAFLVTVDEPSAAAREEGPA"
     gene            2149..2835
                     /gene="rplO"
                     /locus_tag="VIN32_06000"
     CDS             2149..2835
                     /gene="rplO"
                     /locus_tag="VIN32_06000"
                     /inference="COORDINATES: protein motif:HMM:TIGR01071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEY8922165.1"
                     /translation="MKLHDPQPRPGSHQPPRRLGRGHGSGRGKTAGRGTKGQKSRAGG
                     GIPAWFEGGQTPLHIRTPKLHGFKNRFRVEYAPVNLGRLAEAAAGTLITPDVLRHDQL
                     LRDQKTKPMPVKILGGGDAPRGVTIHAHAFTRSALAKLADAGSTAQLISWPDGAPISF
                     ELEPKPEPKGRSKRAAKVAARAAEEDEPVEKEAPADEDAPPAAEAPGAEEGAEEEAET
                     RTADESGSES"
     gene            2837..4120
                     /gene="secY"
                     /locus_tag="VIN32_06005"
     CDS             2837..4120
                     /gene="secY"
                     /locus_tag="VIN32_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEY8922166.1"
                     /translation="MIESLVQAFRAPDIRRKILFTLGILLVFRALTNVPVPNVDQAQL
                     SNLFESNQLLGLLDLFSGGGLATASIIGMGVNPYINASIIMQLMQGVIPSLGELAREG
                     EYGRNRLNQYTRLLTIPMAFAQGYGFSVLLSANKVIPQNPLFSFETLSLLLSFTAGTI
                     LLMWLGELISERGLGNGISFIIFAGIVGRLPHRAAPIVTGPDGLLRIIPFILIAIVVI
                     AGVVFINEGQRRIPVQYASRVRGRKMYQGQTQYLPLKVTMAGVIPIIFAISILLFPAQ
                     IAQYFTASTIDWVKNVASFVVANLSSSNPVVYGILYFVLVVFFTYFYTAFQFRPDQTA
                     DFLRKNGGFIPGIRPGKPTEEFLARITNRITLGGALFLASLAVLPLLVTAAIPGTQGL
                     QLGGTSVLIVVSVVVETMKQLEAQMLMRHYEGFIR"
     gene            4117..4812
                     /locus_tag="VIN32_06010"
     CDS             4117..4812
                     /locus_tag="VIN32_06010"
                     /EC_number="2.7.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050145 - nucleoside monophosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HEY8922167.1"
                     /translation="MTDAGGGHAASPTGRRILLLVGSVGAGKGTQAAALSGELGLAHL
                     ASGNLFRQALRDGSPLGEQARTYMDRGDLVPDDITIQMFMHELAQPAAARGAILDGFP
                     RTVAQARALDATLAERGEQIERVISIEVPVEELVARVAGRRVCPVCGTPYHVDGDPPR
                     VPGRCDRDGAELQQRDDDRPEVVRARLEKQVRPMLEVLDYYDRKGIVQHIDGTQPIPG
                     VTKAILEGLHAGW"
     gene            4806..5573
                     /gene="map"
                     /locus_tag="VIN32_06015"
     CDS             4806..5573
                     /gene="map"
                     /locus_tag="VIN32_06015"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015051599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEY8922168.1"
                     /translation="MVTIKRPEEIARMRHAGTILANVLRVLEGELRPGVATAELDAIA
                     ERMIRDAGATPSFLGYGGSRQTIPFPGAICVSLNDEVVHGIPSTGRRISAGDVVGLDI
                     GCIWQGWHADTARTFAVGPIPERLARLIDATRRGMEAGIAAAVAGNRLGDVGAAIEAI
                     AHEHGYGIVRPFVGHGIGTAMHEDPQVPNYGRPGTGMRIEAGMCFAIEPMFNLGGDDV
                     ALLDDGWTVVTADGTISAHFEDTIAVTPSGPEVLTRA"
     gene            5710..5928
                     /gene="infA"
                     /locus_tag="VIN32_06020"
     CDS             5710..5928
                     /gene="infA"
                     /locus_tag="VIN32_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002614803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEY8922169.1"
                     /translation="MPKKDAIEVEGEVVLPLPNAMFKVRLEDGHEVLAHISGKLRQNF
                     IRILPGDTVRVELSPYDLTRGRITYRMR"
     gene            5925..6038
                     /gene="rpmJ"
                     /locus_tag="VIN32_06025"
     CDS             5925..6038
                     /gene="rpmJ"
                     /locus_tag="VIN32_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEY8922170.1"
                     /translation="MKVSASVKPRCERCKVIKRSGVVMVICVIPKHKQRQG"
     gene            6045..6416
                     /gene="rpsM"
                     /locus_tag="VIN32_06030"
     CDS             6045..6416
                     /gene="rpsM"
                     /locus_tag="VIN32_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEY8922171.1"
                     /translation="MARIAGVDIPREKRVVISLTYIHGIGPSSSQKILTAANVPQATR
                     VRDLTEEEVNRLREVIDKRFKVEGDLRREVSMNIKRLMEIGSYRGVRHRRNLPVRGQR
                     TKTNARQRRGPRKTVGVRRKK"
     gene            6432..6827
                     /gene="rpsK"
                     /locus_tag="VIN32_06035"
     CDS             6432..6827
                     /gene="rpsK"
                     /locus_tag="VIN32_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEY8922172.1"
                     /translation="MAERKKATRTKKREKKNVPTGHAHIQSTFNNTIVTITDMTGNVI
                     SWGSAGLVGFKGSRKSTPYAAAQTADGAAKRAMEHGMRQIEVFVKGPGAGREQAIRSL
                     QTAGLDVTAITDVTPIPHNGCRPPKRRRV"
     gene            6832..7458
                     /gene="rpsD"
                     /locus_tag="VIN32_06040"
     CDS             6832..7458
                     /gene="rpsD"
                     /locus_tag="VIN32_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014344628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEY8922173.1"
                     /translation="MARYTDPVCRICRREGLKLFLKGSRCFSKKCAFERRSNPPGMNT
                     QRRRKVSEFGMQLREKQKVRRSYSVLEKQFRNYFEKAEQRKGMTGENLLRMLEMRLDN
                     VVFRMGFARSRAEARQLVNHRHFAVNGRPTNIPSFGTKIGDRIEVRESRRGREIFKTA
                     AETMKSAAIPEWVSIDAAKLAGSVLSEPAREQMPLEFNEQLVVEYYSR"
     gene            7480..8499
                     /locus_tag="VIN32_06045"
     CDS             7480..8499
                     /locus_tag="VIN32_06045"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006560731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEY8922174.1"
                     /translation="MIELESTPTTRIEEVETRGDLSVFEVNPLADGYGVTLGNALRRV
                     LLNSLEGAAVTSVQVAGVFHEFSTIDNVKEDVTQIVLNIKKLRLRSFARHPVTLKLMK
                     HGAGPVTAADITESADVEIVNPELLLLTLDSDAGSIEMDLTVETGVGYRPAEHAEDLP
                     IGIIPVDAIFTPVRRVNFIVSDTRVGQMTNFDRLSLEIETDGTTTPKAALSSAAEILV
                     QEFSRFAEIGRRPLPGTEDVPALVSANLLDAPIEELDLPMRAYNSLKRNNITKIGQLL
                     ALGDDELLRMRNFGKKSLDEMKERLALRGFIVPESGAGSVDESDLPAVAELDEAVAAE
                     LDRES"
     gene            8515..8856
                     /gene="rplQ"
                     /locus_tag="VIN32_06050"
     CDS             8515..8856
                     /gene="rplQ"
                     /locus_tag="VIN32_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011459109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEY8922175.1"
                     /translation="MAGRRLGRTSNHRLHMLDNLAVSVLRYEKVRTTEAKAKEVRGRV
                     DHMIMLAKRGDLSARRLLISAMPDEPLIIDKLMGELATKYADRASGYTRIVKIGPRLG
                     DAAAIVQIELV"
     gene            8931..9701
                     /gene="truA"
                     /locus_tag="VIN32_06055"
     CDS             8931..9701
                     /gene="truA"
                     /locus_tag="VIN32_06055"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEY8922176.1"
                     /translation="MIEYDGTDFAGSQYQPRMRTVQGELEEALNRLTGERTRVRFAGR
                     TDAGVHATGQVVAFCLARRRRGEGIGWPELRRRLNAALPRDLALRSLQPAAAGFDPRR
                     DARTRVYRYRIRMAGDRRPADRYRTLEIGDRLDVAAMRAVAAELPGERDFGALGSNAE
                     GRTIRHLREVSVVQRGTSIEIRVTANAFLRRMVRSIVALLLEAGRQRLAPGSVAGLLD
                     AGERVLHGRAAPARGLTLERVIYSNTKSATTGSRKKAT"
     gene            9698..10126
                     /gene="rplM"
                     /locus_tag="VIN32_06060"
     CDS             9698..10126
                     /gene="rplM"
                     /locus_tag="VIN32_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012981954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEY8922177.1"
                     /translation="MTTYAVKASEIERSWWVVDAADQTLGRLATRIATLLEGKHKPIY
                     SPHLDTGDHVVVINAAQVKVTGNKLLQKAYYRHSGYPGGLKEESLQALMARKPEIVIE
                     RAVKGMLPQNRLGRAMFKKLHVYRGAEHRHQAQQPTAAEL"
     gene            10141..10533
                     /gene="rpsI"
                     /locus_tag="VIN32_06065"
     CDS             10141..10533
                     /gene="rpsI"
                     /locus_tag="VIN32_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018978338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEY8922178.1"
                     /translation="MSTSYIYGTGRRKEAVARVRLLPGDGSIIVNGKDAADYFGGTPG
                     LATLRLPFRVTETEGRYSASVLVAGGGFSGQAGAIRHGIARALLSAHPDARAALRGAG
                     LLTRDPRAKERKKYGLKRARKAPQYTKR"
     gene            10597..11577
                     /gene="cdaA"
                     /locus_tag="VIN32_06070"
     CDS             10597..11577
                     /gene="cdaA"
                     /locus_tag="VIN32_06070"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HEY8922179.1"
                     /translation="MDAISEFLRLYLLGSWTSVVDILLVAFVIYWVFILIRGTRAVRI
                     VIGLSILYLVYLLAQAAHLRLLSTLLQTGAVVGLFAIVVVFQPELRRALEQIGRFGSF
                     NRFFVSNDVAEAERVAREISRAARLLAGSRHGALIVLERETGLADLAAESGVLLHADL
                     RAELLATLFYHGTALHDGAVIISGDRILAAGVLLPLSQNVLDSERYGTRHRAAIGISE
                     QSDAIVVVVSEETGSISLVMRGRIERNMTEEQLRRRILNLIRPQAPRRQAPLLRRSLE
                     GRWGVAVPPVEAPIDPEARVVKVAGDDKAMIGDATEPQEPAAAAPASKRL"
     gene            11578..12801
                     /locus_tag="VIN32_06075"
     CDS             11578..12801
                     /locus_tag="VIN32_06075"
                     /inference="COORDINATES: protein motif:HMM:NF019561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HEY8922180.1"
                     /translation="MAWLFRNWALKLGAVALATILYTGLVFSGSFSDKTLPGVSVTAL
                     AQPEGTYLLKQQLGTVDIKYRSATNPPASVTAESFAVTIDLSTYDMSQAPQVQSLKIQ
                     VRPLVDGVELLTYEPNTVSVAIDRLAEVQVHVAVDSGQVPPGLEIGTPQLSVQRVTAS
                     GPQSLLDRVDRALATVRIDPSGIDCCGQVDLTPVDADGRRVESVDLNPSTVRVQIDVN
                     TVETSRTVPIRPLLIGAPAAGFEVGTVTADPSVVTLRGAPEVLSAIAEVLTEPISLAG
                     SNSTLRATGTLVLPDGARLADPAAAEPTVVVQIRETVATRTLLLGLVCKGAPPDSACL
                     PHVEQVAGTVRGTVSDLDAIDPGGLTATLDVTGLGPGDHLVQPTVALPKGVTLVTMSP
                     ISVTVTIAPPATPAP"
     gene            12801..14129
                     /gene="glmM"
                     /locus_tag="VIN32_06080"
     CDS             12801..14129
                     /gene="glmM"
                     /locus_tag="VIN32_06080"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEY8922181.1"
                     /translation="MGRLFGTDGIRGVANVDLTPTLAYDLGRAVGHLLEAFGRRVVIG
                     QDTRRSGDMLVAAVSSGLAAVGADAIQLGVVTTPCLAHAAASGEFAAGIMVSASHNPA
                     EDNGLKVLSGGRKIDDEVEEELERLLFQAESLPGMPNGQIGRITRDPRPIDDYRAFLL
                     SEAGDLLRGMRIGIDCANGSASAIAPDLLRELGASVTALSASPDGTNINLDSGSTQPQ
                     ALAAVVLAEGLELGMAFDGDADRLIAVDGRGSIVDGDGVMGICALARLADGTLRNGIL
                     VTTVMSNGGLDRAIDAAGGRVIRTPVGDRHVFEAMERADASLGGEQSGHVIFRDLAVT
                     GDGILTGIQLLRTLRDTGATLAEAAGQIRMLPQVMINSAVRHRDQWEVDPEFAVAVAE
                     ADARLGARGRILVRPSGTEPKIRIMVEGEDADEINQIARELSELARARLN"
     gene            14145..15974
                     /gene="glmS"
                     /locus_tag="VIN32_06085"
     CDS             14145..15974
                     /gene="glmS"
                     /locus_tag="VIN32_06085"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014630348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEY8922182.1"
                     /translation="MCGIVGYVGPRDAAPILLEGLRRLEYRGYDSAGLAVLTDDGEVF
                     VEKKAGKLSNLTDHLNGSAPAGHPGIAHTRWATHGRPNDANAHPHRDCSGRLALIHNG
                     IIENYAEIKERLLAAGHRFTSETDTEVLAHLIEERYQGDLVEAVRDALGEVRGAYAIG
                     VMHLDHPNRIVGARMNVPLIVGLGNGEGFLASDVPAILEHTKNVVILQEGDIADVTSE
                     GVTIVGMDGVQVERPVTRIRWNIAAAEKDGFPHFTLKEIYEQPHAIGEALRGRVDEAG
                     NVKLPELDAIEDKLRRVERVYVVGCGTARYAGEVGAHLIQSWAGLPANGQIGSEMRYS
                     PPPIDDRTLVISVSQSGETADTLAPQKLASERGAVIVVVTNVVGSALTREADAVCYLQ
                     AGPEVAVASTKAFVTQVVVLEMIALHLAQLRGTLSDRRRRSFGLALRDLPGQAAEVLK
                     LAPQVKKLARRWGSVRDVMFVGRALGFPVAMEGALKLKELSYVHAEGYAAGELKHGPI
                     ALLDPQTPLVAIATRSATYEKVVSNVAEVHAREAPVVAVATVGDELIDRYAQDVLYVP
                     ETPEEMSPVIAIVPLQLLAYEVAVARGADVDQPRNLAKSVTVE"
     gene            15994..16374
                     /gene="acpS"
                     /locus_tag="VIN32_06090"
     CDS             15994..16374
                     /gene="acpS"
                     /locus_tag="VIN32_06090"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEY8922183.1"
                     /translation="MSRHEIGVDLIDIDRIIATLGRFPDRFRDRVLTDGEARYCGARA
                     ERIAGRWAAKEAISKVLGLGVRGVGWREIEILPNWAGAPQVRLHARAAARAAAIGLED
                     VTVSISHERHMAVAVAVAHLAPDA"
     gene            16379..>17101
                     /locus_tag="VIN32_06095"
     CDS             16379..>17101
                     /locus_tag="VIN32_06095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEY8922184.1"
                     /translation="MTVVGLSQGWVAEHLPARSAQAHKGTFGKLLIVAGSMEYSGAAL
                     LTGLGAIRAGVGLACLATPEAVGLRLMGLVPELIAMLLVEEAPGLIAPGGWRRLAAEA
                     PAYDALVVGPGIGRQPATLRRARTFITELRHPAVIDADGLVALAEVQRWWQSVRAPLV
                     LTPHPGEFARLMRMSEGLADDDAHRAEAAAEAAARWGQVVVLKGANTVIASPTGDLLR
                     SDVATPALATAGSGDVLAGAIGA"
BASE COUNT         2806 a         5829 c         5915 g         2551 t
ORIGIN      
        1 gccaacatga atgtgactga tccgatcgcc gacatgctga ccaggattcg caatgcgagc
       61 ctggcgcggc atcgtgagct gacgctgccc tcgtcccgca tcaagcgcga catcgcgcgg
      121 atcttggtcg aggaggggtt catcgagtcg tacgccacga gccaggacgg cgtgcaggag
      181 ctcgtgacga tccagctcaa gtacgtcgag ggcaagaccc ccgtcgtcag cggtctgaag
      241 cgaatcagca agccggggtt gcgggtctat gcccgcaaga ccgagatccc gcgcgtcctg
      301 ggcgggctcg gcacggccat cctctcaacc tcccacggga tcatgaccgg gcagagcgct
      361 cggaagctga atctcggcgg cgaggtcctc tgctacgtct ggtaaggagc gaacgcgatg
      421 tcacgcatcg gtcgaatgcc gatcccgctc ccgtcgggcg tggaggtaac ccagcagggc
      481 gcaaccctca gcgtgaaggg tccgctcggc acgctcgagc ggacgatcca ccccgagatg
      541 gcggtcgagc gcgaagaggg cgagctgcgc gtggtgcgcc cgagtgacga gccacgccac
      601 cgggccctgc acggattgac ccggaccctc gtcaacaaca tggtggtcgg cgtgaccacc
      661 gggttcacga aggggctcga gatcagcggg gtcggctatc gggcccagct gcaggggacc
      721 aagctcgtgc tcgcgcttgg ctactcccac cccgtcgagg tcgatccgcc cgaggggatc
      781 agcttccagt tggagacccc gacccggctg gccgtggtcg gggccgacaa ggagctggtc
      841 ggccaggtgg ccgcctacat ccgctcgcgt cgcaagccgg agccctacaa gggcaagggg
      901 atccggtatg ccggcgagca gatcctgcgc aaggccggca aggccggcaa ggtcgggacc
      961 ggcaagtaat gatcaagaag ctttcgcgcg accgactgcg tcgcaagcgt cacgaacgga
     1021 tccggctccg catcaccggc agctcggcgc gcccgcggct ctcgatcttc cgcagcgcca
     1081 agttcatcta tgcccaggtc atcgacgaca gcagcgggcg cacgcttgcc tccgcctcga
     1141 gtcgcgaggc gaccctggcc gccggccccg gcaaggtcga gtcagccaag gcggtcggcc
     1201 tggcgctcgc ccagcgggcg aaggcagcgg gtgtcggcgc cgtggtcctg gaccgcggtg
     1261 gatatcggta tcacgggcgg gttcgctcgc tcaccgaggg cgctcgcgag ggcggcctcg
     1321 acctgtagcg gaggaaccat ggctcgcatc gatccaacca agctcaacct cgaggagaag
     1381 gtcgtccaga tcaaccgcgt cgcgaaggtc gtcaagggcg gccggcgctt cagcttcagc
     1441 gcggtcgtgg tcgtgggcga cggaaacggg atcgtcggcg ccgggctcgg caaggccggc
     1501 gaggtgccgg aggcgatccg caagggggtc gaggatgcaa agaagcacct cgtcaaggtg
     1561 ccgctggtcg gcacctcgat cccgcacatg gtgagcgtcg acttcggcgc cagcaccgtc
     1621 ctcctgcggc cggcctccgc cggcaccggc gtcatcgccg gtggctcggt ccgctccgtc
     1681 gtggaggcgg ccggcattcg cgacatcctc tccaagtcga tgggcagcaa caacccggtc
     1741 aacgtggtgc gcgccacgat cgaggcgctg cggtccctgc gctcggctga cgacatcgcc
     1801 ctgagccggg gcacgaccgc cgagcggctc ctgggcaagc gcggcgcgga agcttcccgt
     1861 cggccacacg agtacgtcga gcgtccggcg tctgcctacc agggaggcgg ccgtggcagg
     1921 taagcgcaag gcgacgagca ctgggccgat gcttcgcgtc acctgggtca agtcgacgat
     1981 cggccacaag gccggtgcgc gcggcaccat ccgggccctc gggctgcatc ggcttcatga
     2041 gaccgtggag gtcaccgaca cccccgtgat ccgcggcatg ctgcgccgcg ttgcattcct
     2101 ggtgaccgtg gatgagccga gcgccgccgc cagggaggag ggcccggcat gaagctgcac
     2161 gatccgcaac cgcgtcccgg ctcgcaccag ccgcctcgcc gcctggggcg tggtcacggc
     2221 tcgggccgag gcaagaccgc gggacgcggc accaagggcc agaagtcgcg cgccggcggc
     2281 ggcattcccg cctggttcga gggtggccag accccgctcc acatccgcac ccccaagctg
     2341 cacggcttca agaaccggtt cagggtcgag tacgccccgg tcaacctggg gcgcctggca
     2401 gaagcggcag ccggaaccct gatcacgcct gacgtcctgc gtcacgacca gctcctgcgc
     2461 gaccagaaga cgaagccgat gccggtcaag atcctgggcg gtggcgacgc cccgcgcggg
     2521 gtcacgattc acgcccacgc cttcacccgc tccgccctcg ccaagctggc ggacgccggc
     2581 agcaccgccc agctcatcag ctggccggat ggcgctccga tctcgttcga gctggagccg
     2641 aagccagagc cgaaggggag gtcgaagcgg gccgccaagg tcgccgcgag ggcggcagag
     2701 gaggacgagc cggtcgagaa ggaagcgccg gccgacgagg acgcgccacc cgccgcggag
     2761 gctccgggcg ccgaagaggg cgccgaggag gaggctgaaa ccaggaccgc tgacgagagc
     2821 ggaagcgagt cttgaggtga tcgagtccct ggtccaggcc ttccgcgccc cggacattcg
     2881 ccgcaagatc ctcttcacgc tcgggatcct gctggtcttc cgggcgctga ccaacgttcc
     2941 ggttccgaac gtggaccagg cgcagctgtc gaacctgttc gagtcgaacc aactgctcgg
     3001 cctgctcgac ctcttctccg gtggcggcct cgcgaccgcg tcgatcatcg ggatgggggt
     3061 caacccctac atcaacgcca gcatcatcat gcagctgatg cagggagtga tcccgagcct
     3121 gggcgagctc gcgcgcgagg gcgagtacgg tcgcaatcgg ctcaaccagt acacccgcct
     3181 gctgacgatc ccgatggcct tcgcccaggg ctacggcttc tcggtgctcc tctcggccaa
     3241 caaagtcatc ccgcagaacc cgctcttcag cttcgagacc ctgtcgctcc tgctctcgtt
     3301 caccgccggc accatcctgc tgatgtggct gggtgagctg atcagcgagc ggggactcgg
     3361 gaacggcatc agcttcatca tcttcgccgg catcgtgggg cggctcccgc accgggccgc
     3421 cccgatcgtc accggtcccg acgggctgct gcgcatcatc ccgttcatcc tgatcgcgat
     3481 cgtggtcatc gccggcgtcg tcttcatcaa cgagggccag cggcggatcc cggtccagta
     3541 cgcgagccgc gtgcggggtc ggaagatgta ccagggacag acccagtacc tgccgctcaa
     3601 ggtgacgatg gccggtgtca tcccgatcat cttcgccatc agcatcctgc tcttcccggc
     3661 gcagatcgcc cagtacttca ccgcctcgac catcgactgg gtcaagaacg tcgcgagctt
     3721 cgtggtcgcg aacctgagct cgagcaaccc ggtcgtgtac ggcatcctct acttcgtgct
     3781 ggtcgtcttc ttcacctact tctacacggc gttccagttc cgtcccgacc agaccgcgga
     3841 cttccttcgc aagaacggcg gcttcatccc cggcatccgg cccgggaagc cgacggagga
     3901 gttcctcgct cgcatcacca accggatcac gctcggcggt gcgctcttcc tggcctcgct
     3961 ggcggtgctg cctctgctgg tcacggccgc gattccgggc acgcagggcc tgcagcttgg
     4021 tggtacgagt gtcctgatcg tggtcagcgt cgtggtcgag acgatgaaac agctcgaggc
     4081 gcagatgctg atgcgccact acgagggctt catccggtga ccgatgcggg cggcggccat
     4141 gcggcgtcgc ccacgggccg ccgaatcctt ctgctggtcg gctcggtcgg cgcgggcaag
     4201 gggacgcagg cggccgctct cagcggcgag ctcggcctgg cgcacctggc ctccggcaac
     4261 ctctttcgcc aggcgcttcg ggatggaagc ccgctcggcg agcaggcgcg cacctacatg
     4321 gaccgcggcg acctcgtccc cgacgacatc accatccaga tgttcatgca cgagctcgcg
     4381 cagccggccg cggctcgcgg tgccatcctc gacggcttcc cccgcacggt ggcgcaggct
     4441 cgcgccctcg acgccaccct cgctgagcgc ggcgagcaga tcgaacgcgt catctccatc
     4501 gaggttccgg tcgaggagct ggtggccagg gtcgcgggac gccgcgtctg cccggtgtgc
     4561 ggaactccgt accacgtcga cggcgacccg ccgcgcgtgc ccggccggtg cgacagggac
     4621 ggcgccgagc tgcagcagcg cgacgatgat cgacccgagg tcgtccgcgc ccggctcgag
     4681 aagcaggtcc gcccgatgct cgaagtgctc gactactacg accggaaggg gatcgtgcag
     4741 cacattgacg gcacccagcc gatccccggc gtgacgaaag cgatcctcga gggcctccac
     4801 gccggatggt gaccatcaag cgccccgagg agatcgcgcg catgcggcat gcggggacga
     4861 tcctggcgaa cgtcctgcgc gtgctcgagg gcgagctgcg cccgggcgtc gcgaccgccg
     4921 agctcgacgc catcgccgag cggatgatcc gcgacgcggg ggcgaccccc tcattcctgg
     4981 gctacggcgg ctcgcgccag accatcccgt tccccggagc gatctgcgtc tcgctcaacg
     5041 acgaggtggt gcacggcatc ccgtccacgg gccgccgtat cagcgccggc gacgtggtcg
     5101 ggctggacat cggctgcata tggcagggct ggcatgccga tacggcgcgc accttcgcgg
     5161 tcgggccgat ccccgagcga ctggctcggc tgatcgatgc cacacggcgt ggcatggagg
     5221 ccgggatcgc ggcggcagtc gcggggaacc gcctgggcga cgtcggcgcc gccatcgagg
     5281 cgatcgctca cgagcacggc tacggcatcg tccgaccgtt cgtgggccat ggcatcggga
     5341 ccgccatgca cgaggatccg caggttccga attacgggcg ccctgggacc gggatgcgga
     5401 tcgaggcggg catgtgcttc gcgatcgagc ccatgttcaa cttgggcggc gacgacgtgg
     5461 cgctgctgga cgatggctgg acggttgtca ccgccgacgg caccatctcg gcccatttcg
     5521 aggacaccat cgcggtcacg ccatccggcc cggaggtgct cacccgtgcc tgatcgccgc
     5581 tctgatcgac gttgtgctcg gcaccctgcc tcgtgtatac tcctccttcc atggtcgccc
     5641 cgagcggcga cctttccgtg tgttcccatt ccatttgcag ctaatcagca gttcgagggt
     5701 gcgtagcgct tgccgaagaa ggatgcgatc gaggtcgagg gcgaggttgt gctccccttg
     5761 cccaacgcga tgttcaaggt gcgactggag gatggtcacg aggttctcgc ccacatctcc
     5821 ggcaagctgc gccagaactt catccgcatc ctgcccggcg ataccgtgcg cgtcgagctg
     5881 agtccctatg acctcaccag gggtcgcatc acatacagga tgagatgaag gtttctgcct
     5941 cggtcaagcc gcgctgtgag cggtgcaagg tcatcaagcg ctcaggcgtg gtgatggtca
     6001 tctgcgtcat ccccaagcac aagcagcgac agggctaggc gcgggtggca cgcatcgccg
     6061 gtgtcgacat tccccgcgag aagcgggtcg tcatctcgct gacctacatc cacggcatcg
     6121 gtccaagctc atcgcagaag atcctgacgg cagccaacgt gccccaggcg acgcgcgtcc
     6181 gcgatctcac cgaggaggag gtcaaccgcc tccgcgaggt gatcgacaag cgcttcaagg
     6241 tggagggcga cctccgacgc gaagtgagca tgaacatcaa gcgcctgatg gagatcggct
     6301 cctaccgggg cgttcgccat cgacgcaatc tcccggtgcg ggggcagcgc accaagacga
     6361 acgcacgtca gcggcgcggt ccgcgcaaga cggtcggcgt tcggcgcaag aagtagcgac
     6421 acaggagttc catggctgag cgaaagaagg cgacccgaac caagaagcgc gagaagaaga
     6481 acgtgccgac cgggcacgcc cacatccagt cgaccttcaa caacaccatc gtgaccatca
     6541 cggacatgac cgggaacgtg atcagctggg ggagcgccgg actggtcggg ttcaagggct
     6601 cgcgcaagag caccccgtac gccgcggccc agaccgccga cggggcggcg aagcgcgcca
     6661 tggagcacgg aatgcgccag atcgaggtct tcgtcaaggg cccgggtgcc ggtcgcgagc
     6721 aggccatccg ctccctgcag accgccggcc tggacgtgac cgccatcacc gatgtcacgc
     6781 cgatcccgca caacggctgc cgccctccca agcggcgcag ggtctgagcc gatggcacgc
     6841 tataccgatc cggtctgccg catctgccgt cgcgaggggc tcaagctctt cctgaagggg
     6901 agccgctgct tcagcaagaa gtgcgccttc gaacggcgca gcaacccacc gggaatgaac
     6961 acccagcgcc gccgcaaggt cagcgagttc gggatgcagc tgcgcgagaa gcagaaggtg
     7021 cgccgcagct actcagtcct ggagaagcag ttccgcaact atttcgagaa ggccgagcag
     7081 cgcaagggga tgacgggcga gaacctgctc cgcatgctcg agatgcgcct cgacaacgtc
     7141 gtattccgca tgggctttgc gcggtctcgg gccgaggcgc gccagctcgt caaccaccgg
     7201 cacttcgccg tgaacggccg gcccaccaac atcccgagct tcgggaccaa gatcggcgac
     7261 cggatcgagg tccgggagag ccgacgcggt cgcgagatct tcaagaccgc ggccgagacg
     7321 atgaagtcgg cagcgatccc cgagtgggtg agcatcgatg cggccaagct ggccgggtcg
     7381 gtgctgtccg agcccgcgag ggagcagatg ccgctcgaat tcaacgagca gctcgtcgtc
     7441 gagtactact cgcgatagca gcaggagtca tacgccttca tgatcgaact tgagtccacg
     7501 ccgaccaccc gcatcgagga ggtcgaaacg cgcggcgacc tgtcagtctt cgaggtcaac
     7561 ccgctggccg atggctacgg ggtgacgctc ggcaacgcgc tgcgccgcgt cctcctgaat
     7621 tcgctcgagg gggccgccgt cacatcggtg caggtggccg gagtcttcca cgaattcagc
     7681 accatcgaca acgtcaagga ggacgtgacc cagatcgtcc tcaacatcaa gaagctgcgc
     7741 ctgcgctcgt tcgcgcgcca cccggtcacg ctcaagctga tgaagcatgg tgccggaccg
     7801 gtgactgccg ccgacatcac cgagtcggcc gacgtggaga tcgtcaaccc ggagctgctg
     7861 ctgctcacgc tcgactcgga cgccgggtcg atcgagatgg acctgaccgt tgagaccggg
     7921 gtcggctatc gacccgccga gcatgccgag gatctgccga tcgggatcat cccggttgac
     7981 gccatcttca ccccggtgcg gcgcgtgaac ttcatcgtca gcgacacccg cgttggtcag
     8041 atgaccaact tcgaccgtct gagcctcgag atcgagaccg atggcacgac gacccccaag
     8101 gcagcgctgt cgagtgcagc cgagatcctg gtgcaggagt tcagccgctt tgccgagatc
     8161 ggtcggcggc cgctgccggg caccgaggac gtcccggccc tggtgagcgc gaacctgctc
     8221 gacgcgccga tcgaggagct cgacctgccg atgcgcgcct acaacagcct gaagcgcaac
     8281 aacatcacca agatcggcca gctcctggcg ctcggtgacg acgagctgct gcggatgcgc
     8341 aacttcggca agaagagcct cgacgagatg aaggagcgcc ttgcgctgcg cggcttcatc
     8401 gtgccggaga gtggcgcggg gagcgtcgac gagtccgacc tgccggccgt cgccgagctg
     8461 gatgaggcgg tcgccgccga gctcgatcga gagagttgag cgatgcccca tcggttggcc
     8521 ggtcggcggc ttggccgcac aagcaaccat cgcctgcaca tgctggacaa cctggcggtg
     8581 tcggtcctgc gctatgagaa ggtccggacc accgaggcca aggccaagga ggttcggggc
     8641 cgcgtggacc acatgatcat gctggcgaag cgcggcgacc tctcggcccg ccgcctcctg
     8701 atctccgcca tgccggatga gcccctcatc atcgacaagc tgatgggcga gctggccacc
     8761 aaatacgccg accgtgcctc cggctacacc cgcatcgtca agatcgggcc gcggctggga
     8821 gatgcggcgg ccatcgtcca gatcgagctg gtctagcgag acccgcggcg actgggcgga
     8881 caatggaaga cgtaccgatg ccacccactg gacgacgatg tgcacgggcg gtgatcgagt
     8941 acgacgggac cgacttcgcc ggctcccagt accagccgcg gatgcggacg gtgcaggggg
     9001 agctggagga ggcgcttaac agactgaccg gcgagcggac gagagtccgg ttcgccgggc
     9061 gaaccgatgc cggggtgcac gccaccggcc aggtcgtggc cttctgcctc gctcgccgcc
     9121 gtcgcggaga ggggatcgga tggccggagc tgcggcgaag gctgaatgcg gccctcccgc
     9181 gggacttggc actgcggtcg ctccaaccgg cggccgcggg attcgacccg cggcgcgacg
     9241 ctcgcacgcg cgtctaccgc taccgaattc ggatggcggg cgaccggcgg ccagcggacc
     9301 ggtatcggac gctcgagatc ggcgatcggc tcgacgtcgc ggccatgcgg gccgtggcgg
     9361 cggagctccc cggggagcga gacttcggag ccctggggag caacgcggag ggtcgaacca
     9421 tccgccacct gcgggaggtg tcggtcgtgc agcgcggaac ctcgatcgag atccgggtca
     9481 cggccaacgc gttcctgagg cgaatggtgc gcagcatcgt ggccctcctc ctggaggcgg
     9541 ggcggcagag gctggcaccg ggatcggtcg ccgggttgct cgatgcgggt gagcgtgtcc
     9601 tgcatggacg cgcggcaccg gcacgcgggc tcacgctcga gcgggtcatc tattcgaata
     9661 caaagtcggc gactacggga tcgaggaaga aggcaacgtg acaacgtacg cagtcaaggc
     9721 gagcgagatc gagcggagct ggtgggtggt tgacgccgcc gaccagacgc tgggtcgcct
     9781 cgccacgcgc atcgccacgc tgctggaggg caagcacaag ccgatctact cgccgcacct
     9841 cgacaccggg gaccacgtgg tggtcatcaa cgcggcgcag gtcaaggtga ccggcaacaa
     9901 gctgctgcag aaggcctatt accggcactc gggctacccc ggcgggctga aggaggagag
     9961 cctgcaggcc ctcatggcgc gcaagcccga gatcgtgatc gagcgggccg tgaaggggat
    10021 gctgccccag aaccgcctcg gtcgggcgat gttcaagaag ctccatgtct atcgcggggc
    10081 ggagcatcgc caccaggcac agcagccgac cgccgcagag ctgtaagagg aacgcattcg
    10141 gtgagcacca gctacatcta cggaaccggc cgacgcaagg aggcggtcgc gcgcgtgcgg
    10201 ctcctgccgg gcgacggcag catcatcgtg aacggcaagg atgccgccga ctacttcggc
    10261 ggcacgcccg ggctggccac cctccgcctt ccattccggg tgaccgagac cgagggtcgc
    10321 tactcggcca gtgtgctggt cgcagggggc ggcttctccg gccaggctgg agcgatccgc
    10381 cacgggatcg cacgggcgct gctcagcgcc caccccgacg cacgggccgc gctgcggggg
    10441 gcgggactcc tgacgcgcga tccgcgagcc aaggagcgca agaagtacgg cctcaagcga
    10501 gcccgcaagg caccgcagta caccaagcgc tgacagcggc gtaccgaccg gtggggacgg
    10561 gcggtcggag gccctttggt atcatcggtg cccttgttgg acgccatctc tgagttcctc
    10621 aggctttacc tgctcggcag ctggacgtcg gtcgtcgaca tcctgctggt ggcattcgtc
    10681 atctactggg tcttcatcct gatccggggc acccgagcgg tgcgcatcgt catcggtctc
    10741 tcgatcctgt acctcgtcta cctgcttgcc caggccgcgc acctgcggct cctgtcgacc
    10801 ctcctgcaga ccggcgctgt ggtcggtctc ttcgccatcg tcgtcgtctt ccagccggag
    10861 ctgcggcgcg cgctggagca gatcggccga ttcgggtcct tcaaccgctt cttcgtgagc
    10921 aacgatgtcg ccgaggcgga gcgggttgcc cgggagatca gccgggccgc gcgcctgctg
    10981 gccggatcgc gacacggtgc cctcatcgtg ctcgagcgcg agaccgggct cgccgacctg
    11041 gccgccgagt ccggggtgct cctgcacgcc gaccttcggg cggagctgct ggcgaccctc
    11101 ttctatcacg ggacggcgct gcatgacggc gcggtgatca tctccggcga ccgcatcctg
    11161 gcggccggag tgctgctgcc gctctcccag aacgtgctcg attcggagcg ctacggcacc
    11221 cgtcaccgcg cagccatcgg gatcagcgag cagtcggatg caatcgtggt ggtcgtctcg
    11281 gaggagacgg ggtcgatctc cctggtgatg cgcgggcgga tcgagcgcaa catgaccgag
    11341 gagcagctgc gaaggaggat cctcaacctc attcgcccgc aggctccccg tcgccaggca
    11401 cccctcctgc ggcgctccct cgagggccgc tggggggtcg ccgtcccacc tgtggaagcg
    11461 ccgattgacc ccgaggcccg cgtcgtcaag gtggcgggcg acgacaaggc gatgatcggc
    11521 gacgcgacgg agccgcagga gcccgcggcc gcagcccccg cctcgaagcg cctctagatg
    11581 gcctggctgt ttcgcaactg ggccctgaag ctgggcgcgg tcgccctggc gacgatcctg
    11641 tacaccggcc tggtcttctc cggctcgttc agcgacaaga ccttaccggg cgtctccgtc
    11701 acggccctcg cgcagcccga ggggacgtac ctgctgaagc agcagctggg cacggtcgac
    11761 atcaagtacc gcagcgcaac caacccaccg gccagcgtga ccgccgagtc cttcgccgtc
    11821 accatcgacc tctccaccta cgacatgtcc caggcgcctc aggtgcagtc gctcaagatt
    11881 caggtccgtc ccctcgttga cggggtcgaa ttgctcacct acgagccgaa cacggtttcc
    11941 gtggcgatcg accgccttgc cgaggtgcag gtccacgtgg ccgtcgattc gggccaggtc
    12001 ccgcccggcc tggagatcgg caccccccag ctcagcgtcc aacgggtcac cgccagcggc
    12061 ccccagagcc tcctggaccg cgttgaccga gcgctggcca cggtacgcat cgacccgtcc
    12121 gggatcgact gctgcgggca ggtcgatctg acaccggtcg acgccgatgg gcgccgcgtc
    12181 gaatcggtgg atctgaaccc gtcgacggtc cgggtccaga tcgacgtcaa tacggtcgag
    12241 accagccgca ccgtgccgat tcgtccgctg ctgatcggtg cgcccgccgc cggcttcgag
    12301 gtcgggacgg tgaccgcaga cccatccgtg gtcacgctgc gcggcgcacc ggaggtcctc
    12361 tcggccatcg ccgaggtgtt gacggagccg atcagcctgg cgggcagcaa ctccaccctg
    12421 cgagcgaccg ggacgctggt gctgcccgac ggggcccggc tcgcggatcc cgcggccgcc
    12481 gagccgacgg tggtggtgca gattcgtgag acggtcgcca ccaggacgct cctgctcggc
    12541 ctggtctgca agggtgcccc gcccgacagc gcctgcctgc cgcacgtcga gcaggtcgcg
    12601 ggcacagtgc gtgggacggt ctcggacctc gacgccatcg accctggggg cctgaccgca
    12661 actctcgatg tgacggggct tggtccaggc gatcacctgg tccagccaac cgtcgcactt
    12721 ccgaagggag tgacgctcgt caccatgtcg ccgatctcgg tcacggtcac gatcgctccg
    12781 ccggcgacgc cggcgccctg atgggtcgcc tcttcggaac cgacggaatt cgcggtgtcg
    12841 ccaatgtcga cctgacgccc accctggcct acgacctggg gcgcgccgtc ggccacctgc
    12901 tggaggcgtt cgggcgtcgc gtggtgatcg gccaggacac gcgccgctcc ggcgacatgc
    12961 tcgtagccgc cgtctcctcg ggcctggccg ccgtgggcgc agacgccatc cagcttggcg
    13021 tggtgaccac gccgtgcctg gcgcatgcgg ccgcgagcgg tgagttcgct gccgggatca
    13081 tggtctcggc ctcccacaac cccgccgagg acaacgggct gaaggtgctc tcgggcgggc
    13141 ggaagatcga cgacgaggtg gaggaggagc tcgagcgcct gctcttccag gccgagtcgc
    13201 tgcccggcat gccaaatggg cagatcgggc ggatcacgcg cgacccgcgg ccgatcgatg
    13261 actaccgcgc cttcctgctt tcggaggcgg gcgatctgct gcgcgggatg cggatcggga
    13321 tcgactgtgc caacggctct gcctccgcga tcgctcccga cctgctgcgc gagctggggg
    13381 cgagcgtcac ggcactgtcg gcgtcccccg acgggaccaa catcaacctc gattccggct
    13441 cgacccagcc gcaggcgctt gccgccgtcg tgctggccga gggcctcgag ctggggatgg
    13501 cattcgacgg tgacgccgac cggctgatcg ccgtcgacgg acgcggctcg atcgtcgatg
    13561 gcgacggggt gatgggcatc tgcgccctgg ctcgcctggc ggacgggacg ctgcgcaacg
    13621 ggatcctggt cacgaccgtg atgagcaacg gcggactcga ccgcgccatc gacgctgccg
    13681 gagggcgcgt gatcaggaca ccggttggcg atcggcatgt gttcgaagcc atggagcggg
    13741 ccgacgcatc actcggcggc gagcaatcgg ggcacgtcat ctttcgcgac ctggccgtga
    13801 ccggcgacgg gatcctcacc ggcatccagc tgctccggac gcttcgcgac acgggcgcca
    13861 cgctggccga ggcggccggc cagatccgga tgcttccgca ggtgatgata aactcggccg
    13921 tccgccatcg ggaccagtgg gaggtcgatc cggagttcgc agtcgcggtg gcagaggccg
    13981 acgcccgtct tggcgcgcgt ggacggatcc tggtgcggcc ctccgggacg gagccgaaga
    14041 tccggatcat ggtcgagggc gaggacgctg acgagatcaa ccagattgcg cgagagcttt
    14101 ccgagctcgc ccgcgcccga ttgaactagg agccaccaga aaccatgtgc ggcatcgtgg
    14161 ggtacgtcgg cccgcgtgac gcggcgccga tcctgctcga ggggcttcgc cgcctcgagt
    14221 accgaggcta cgactcggcc gggctggcag tcctgaccga cgatggggaa gtgttcgtcg
    14281 agaagaaggc tggcaagctc tccaacctga ccgaccacct gaacggcagc gccccggcgg
    14341 gacatcccgg catcgcccac acccgctggg ctacccacgg acgcccgaac gacgccaatg
    14401 cccatcccca tcgcgactgc agcgggcgcc tggcgctgat ccacaacggg atcatcgaga
    14461 actacgccga gatcaaggag cggctgctgg ccgccggcca tcggttcacc tccgagaccg
    14521 ataccgaggt cctggcgcac ctgatcgagg agcgctacca gggggacctc gtcgaggcgg
    14581 tgcgcgatgc cctcggtgag gtgcgcggcg cctacgccat cggcgtgatg cacctcgacc
    14641 accccaatcg gatcgtcggg gcgcgcatga acgtgccact gatcgtcgga ttggggaacg
    14701 gtgagggttt cctggcgagc gacgtgccgg cgatcctgga gcacaccaag aacgtcgtca
    14761 tcctgcagga gggcgacatc gccgatgtga cttcggaagg ggtcaccatc gtcggcatgg
    14821 acggggtcca ggtggagcgg ccggtcacca ggatccggtg gaacatcgct gcggcggaga
    14881 aggacggctt cccgcacttc accctcaagg agatctacga gcagccgcat gccatcggcg
    14941 aggccctgcg cggtcgggtc gacgaggcgg ggaacgtcaa gctccccgag ctggacgcga
    15001 tcgaggacaa gctgcggcgg gtcgagcgcg tgtacgtggt gggctgcggc acggcccgct
    15061 acgcgggcga ggttggggcg cacctgatcc agagctgggc gggtctgccc gcgaacggcc
    15121 agatcggctc cgagatgcgg tattcgccgc cccccatcga cgaccggacc ctcgtcatca
    15181 gcgtcagcca gtcgggcgag acggccgaca cgctggcgcc ccagaagctc gcatccgagc
    15241 gaggtgccgt gatcgtggtc gtcaccaacg tggtcggcag cgccctcacc cgcgaggccg
    15301 atgcggtctg ctacctgcag gccgggcccg aggtggcggt ggcgtcgacc aaggcgttcg
    15361 tcacgcaggt cgtggtgctc gagatgatcg cgctccacct cgcgcaactg cgaggcacgc
    15421 tgagcgaccg gcgccgccgc tcgttcgggc ttgccctgcg cgacctcccc gggcaggcgg
    15481 ccgaggtgct caagctggct ccgcaggtca agaagctggc caggcgctgg ggcagcgtgc
    15541 gcgacgtcat gttcgtcggt cgggcgctcg gattcccggt cgccatggag ggagccctga
    15601 agctcaagga gctctcctac gtccacgccg aggggtatgc cgccggcgag ctgaagcatg
    15661 gaccgattgc gctgctcgac ccgcagaccc cgctggtggc gatcgcgacc aggagcgcga
    15721 catacgagaa ggtggtcagc aacgtcgccg aggtgcatgc ccgcgaggcg ccggtggtgg
    15781 cggtggcgac ggtcggggac gagttgatcg atcgctacgc ccaggacgtc ctgtacgtcc
    15841 ccgagacacc cgaggagatg tcccccgtga tcgcgatcgt gccactgcag ctgctcgcct
    15901 acgaggtcgc cgtggcgcga ggcgcggacg tcgaccagcc ccgcaacctg gcgaagtcgg
    15961 tcacggtcga atagccgtcg ccacactgcc gcgatgagcc gccacgagat cggggtcgac
    16021 ctgatcgaca tcgatcgcat catcgcgacg ctcggccgct ttcccgatcg attccgcgac
    16081 cgcgtcctga ccgacggcga ggctcgctac tgcggtgcgc gggccgagcg cattgccggg
    16141 cgctgggcag ccaaggaggc gatcagcaag gtgcttggcc tgggcgtgcg cggcgtcggt
    16201 tggcgggaga tcgagatcct gcccaactgg gccggtgccc cacaggtccg cctccacgcg
    16261 cgggctgcgg cccgcgcggc cgcgatcggg ctggaagacg tgaccgtcag catctcgcac
    16321 gaacggcaca tggcggtggc ggtggcggtc gcgcacctcg cgccggacgc ataggccgat
    16381 gacggtagtg ggactgtccc aggggtgggt cgccgagcac ctcccggcgc gttctgcgca
    16441 ggcccacaag ggcaccttcg ggaagctgct gatcgtggcg ggctcgatgg agtattcggg
    16501 tgcggcgctg ctgaccgggc tgggtgccat ccgcgcgggg gtcggcctgg cctgcctggc
    16561 cacgccagag gcggtgggcc tccggctgat ggggctcgtc cccgagctga tcgcgatgct
    16621 cctcgtcgag gaggcgcccg gtctcatcgc accgggtggc tggcgtcgcc tggccgccga
    16681 ggctccggcg tatgacgcgc tggtggtcgg tcccgggatc ggccggcagc cggccacgct
    16741 gcgtcgggca cgcaccttca tcactgagct ccgccacccg gcggtgatcg acgccgacgg
    16801 cctggtggcg ctcgcggagg tccagcgctg gtggcagtcc gtccgggcgc ccctggtcct
    16861 caccccgcac cccggcgagt ttgcccgact gatgcggatg tcggaggggc tggccgatga
    16921 cgatgcccat cgagcggagg cggctgccga ggcggcggcc cgctggggcc aggtcgtcgt
    16981 cctcaagggc gcgaacaccg tcatcgcctc gccgaccggc gacctcctcc ggtccgacgt
    17041 cgccacgccg gcgctggcca ccgccggaag cggtgacgtg ctggcgggcg cgatcggagc
    17101 a
//
LOCUS       DATCQR010000181         2489 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_133949, whole genome shotgun sequence.
ACCESSION   DATCQR010000181 DATCQR010000000
VERSION     DATCQR010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133949"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(264..866)
                     /locus_tag="VIN32_06100"
     CDS             complement(264..866)
                     /locus_tag="VIN32_06100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8922185.1"
                     /translation="MRLTILQRGHRLRQRVALFAIWLVGRTEPDPVAKLSLYRPEFFG
                     RSWVRLIESTMRGPSEWSDADREVMGAFVSRINECPFCVGIHEGTAALLTGSSSIAEQ
                     LERWREPDFDPRLGATLGLLEKVTIAPDRVSATDVAAVRAAGVSPPAIADALYVCFLF
                     NTVNRLANAFDFAWATEADRVKLARGLNRIRYHIPEFLLT"
     gene            complement(868..1164)
                     /locus_tag="VIN32_06105"
     CDS             complement(868..1164)
                     /locus_tag="VIN32_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020785305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8922186.1"
                     /translation="MGHADEVAELRRSVLEGRARSDPALRAAAFSGEHVPEPWTAYVT
                     KVRNQSYRVTDEDVARLTAGGATEDSIFELTVAAALGAAALRLKAGLAALSEAD"
     gene            complement(1418..2119)
                     /locus_tag="VIN32_06110"
     CDS             complement(1418..2119)
                     /locus_tag="VIN32_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018635913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8922187.1"
                     /translation="MLDLASTLSRMPEVRRPAPWAGYEYVKGWAVFGLPFDSGHVLAL
                     RVMPESSIAPYRSLYHRDPLGNWALYVDGPKVCACTRYYGAACNLTGHTRIQLEWTAP
                     ATLRVTLDQPPLDWTLTATSDRRLDLINAVSAVLPLSSWRSSLLLRARERLAKAMGLG
                     ELELSGTTPSGDVETLMPEQIYFIEESSATLDGVDLGRPVHLHENPMLGTLPLPARGI
                     LVRGQGMWQIRGTGA"
BASE COUNT          443 a          847 c          790 g          409 t
ORIGIN      
        1 aagccgtacc ccctccccac gcccattgca tgctgagcca ggtcagcgcc gcctgccccg
       61 tatttcctaa aggattacag acgctcgccg aagaagttca attccccacc ctcccaccaa
      121 actcagcttt gagttcggcc cggcatccca gacgacgtgt tggacgcaat tggcaggttc
      181 gtggccgaac gaagtcaacg ctggctcacc caggcgccgc cagccagtca tcaaggcggt
      241 ccggcccgag cgctgctccc tcgtcaggtc agcaggaact ccgggatgtg gtaacggatc
      301 cggttgagac ctcgcgccag cttcacgcgg tcggcctcgg tcgcccacgc gaagtcgaac
      361 gcgttggcga gccggttgac ggtattgaac aggaagcaga cgtagagcgc gtccgcgatc
      421 gccggcggcg acacacccgc ggcacgcacg gccgccacat ctgtagcgct gacgcgatct
      481 ggcgcaatcg tcaccttctc gagcaggcct aatgtcgctc cgaggcgcgg gtcgaagtca
      541 ggttcgcgcc agcgctcgag ttgctcggcg atcgaggatg acccggtcag cagcgcggct
      601 gtgccctcgt ggatcccgac gcagaagggg cactcgttga tccgggacac gaaggcgccc
      661 atgacctccc ggtcggcgtc cgaccactct gacggcccgc gcatcgtcga ttcgatgagg
      721 cggacccaac tccggccgaa gaactcgggc cggtagagcg acagtttcgc cacggggtcc
      781 ggttcagtcc gcccgaccag ccagatggcg aaaagcgcga cccgctgccg aagccggtga
      841 ccgcgctgca ggattgtaag ccgcacctca gtcggcctcg gagagcgcgg ccaatcccgc
      901 cttgagccgt agggcggcgg cgccgagcgc cgcggcgacc gtcagctcga atatcgagtc
      961 ttcggtcgct ccgcccgccg tcagcctggc gacgtcctca tccgtcacgc gataggactg
     1021 attgcgcacc ttggtgacgt aggcggtcca cggctccggc acgtgctccc cggaaaaggc
     1081 agctgcacgc agcgccgggt cgctcctggc gcgaccctcc agcaccgaac gccgaagctc
     1141 cgccacctcg tccgcgtggc ccatctgccg ccgaagggta gccgttcagg ctgcggatcg
     1201 cgtccagtag atcggttttg gtcggacata gttccgcgcg cccgtctccc gctccacttc
     1261 gtggatcctc ccggcacgca gcgcgacgcg cgagtatcgg cccctacctc agctggactt
     1321 gaaccagaag cctccggcgt gccccgaatt ccttaaggtg tacggactca gcgggcccga
     1381 ggtgcacggc cacgccttga cgtgttaatc ggtggtacta cgcgccggtg ccgcggatct
     1441 gccacatccc ttgcccacgc acgagaatgc cgcgagcagg gagaggcaat gtaccgagca
     1501 tgggattctc atgaaggtga acaggacggc cgaggtcgac cccatcgagg gtggcgctgg
     1561 actcctcgat gaaatagatc tgctccggca tcagggtttc gacgtcacca ctgggcgtag
     1621 tgcccgaaag ctccagctcg ccaaggccca tcgctttggc cagacgctca cgagcccgca
     1681 gcagcaggct cgagcgccac gaggaaagag gcaacacggc gctgaccgca ttgattaggt
     1741 cgagccggcg atcagaggtc gcggtcagcg tccagtcgag cggtggctga tccagggtga
     1801 cgcgtaatgt tgccggcgct gtccactcga gctgtattcg cgtgtggccc gtgaggttac
     1861 atgcggctcc gtaatacctg gtacacgcgc agaccttcgg tccgtcgaca tagagcgccc
     1921 agttcccaag cggatctcga tggtagagcg accggtaggg agcgatgctg ctctccggca
     1981 taacgcgcag ggccaggacg tgtccggaat caaacggaag gccgaagacg gcccagccct
     2041 tgacgtattc gtaaccggcc caaggtgccg ggcggcgcac ttccggcatg cgagacaagg
     2101 tcgaggccag gtcgagcatt ctttttcgct tccctccttc ttagggcggt ggcccgcacg
     2161 tgaccgccgg tatggcaccc ggctaggatc gccgactgct cgtcagcgtc agctggtcgg
     2221 tggacgtcat cgattcacag tcgcgccact gttggccgcc acctcgacgt ctcttccggc
     2281 gtcggcccat tctggcaacc cgtcggcgag gtatcgggca gcgtagccgt gctcgcggag
     2341 ggtacgaacc gcttctggcg caaaggcgcg gtagggccgc ggcaataagc cacgacttcg
     2401 cggtccggtg gtagctcccg caacctccgc tccaactccg ccagcggagt cgacaccgcg
     2461 ccggcaacat gcgccgctcg gtacaagcg
//
LOCUS       DATCQR010000182         3886 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_41447, whole genome shotgun sequence.
ACCESSION   DATCQR010000182 DATCQR010000000
VERSION     DATCQR010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3886
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41447"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="VIN32_06115"
     CDS             <1..298
                     /locus_tag="VIN32_06115"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017999457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEY8922188.1"
                     /translation="RFDLPIVAPGSAFQERIWAELQRLAAGTTLSYEELAQRLGRPRT
                     QRAVGTANGANRIAVVIPCHRVVRKTGETGNYGGGRWRKEWLLAHEAGLAPAPA"
     gene            complement(295..1077)
                     /gene="fabI"
                     /locus_tag="VIN32_06120"
     CDS             complement(295..1077)
                     /gene="fabI"
                     /locus_tag="VIN32_06120"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HEY8922189.1"
                     /translation="MLLDGKKLLITGVLTDDSIAFSIAKVAQEAGAEVLLTGVGRGMS
                     LTQRVAKKLPTTPDVLELDVNNPEHIEALSAELMKRWGKVDGVVHAIGFMPSDGLGGN
                     FLNTPWESVAFGFQTSAYSLKALSVGLLEPMKAAGSASVVSLTFDGRFAWPIYDWMGV
                     AKAGLEAVTRYLARDLGEHHIRVNTVSAGPIRTMAGKSIPGFDQIADTWLKRAPLGWD
                     MNDATAVGQMVCFLLSDWAAKTSGELIHVDGGYHAMGTDIRR"
     gene            1124..1585
                     /locus_tag="VIN32_06125"
     CDS             1124..1585
                     /locus_tag="VIN32_06125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8922190.1"
                     /translation="MMVHLYDYEVPVADAATAMPSLREALSALGDEDRKALLGGLFKQ
                     ERMPGTMERLSTLVSDLDASFGDLLTLLDNSARARRFVAIDEAKDVLDRYLPANAVSG
                     THDWDVARLHEPYGDGTYHGKLLKRVRKQLRSVGTREALTTPFLLRREWGR"
     gene            1590..1730
                     /locus_tag="VIN32_06130"
     CDS             1590..1730
                     /locus_tag="VIN32_06130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8922191.1"
                     /translation="MSRRSQRRGRPYQPLPARQVNWGARLVIVFVAFVLVAGFAILSF
                     AR"
     gene            complement(1932..2375)
                     /locus_tag="VIN32_06135"
     CDS             complement(1932..2375)
                     /locus_tag="VIN32_06135"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY8922192.1"
                     /translation="MLRPLRIGHVVLKVRDLDRSLAFYRDLLGFKVVSELSNVMIFLA
                     ADGQNHHDLALLRVGQQAASPVPTAVGLYHVAIQLADLDAVRTAHALLTERGLLRGAV
                     DHGVSRSLYTADPDGNEIELYCDAPRSEWEGRIDEVMTVKPLVID"
     gene            complement(2419..3060)
                     /locus_tag="VIN32_06140"
     CDS             complement(2419..3060)
                     /locus_tag="VIN32_06140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8922193.1"
                     /translation="MPDLLARVAVRTIVFAVVIGLMAPLSARSATPASAESMRLLAVA
                     DQPLSLADRSSLDGRNRSASLSLSGVSQPIGTEDLGLAPAPATPPTIAMEASLYPAPA
                     KKVAPKSVAAPVSGDTVSGEATWYCCSAGWRGQAVVALPGALGGHYDAPPAARFVTVC
                     ADRCAVLPVADYCGCLWGTADQKVADLSPEAWAAISDSSTSRGVITVTVHLGG"
     gene            3216..>3886
                     /locus_tag="VIN32_06145"
     CDS             3216..>3886
                     /locus_tag="VIN32_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015883413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEY8922194.1"
                     /translation="MATKRNQAHRSGAVVMRGDEKRPTEDEQLLQQEGTDASFLKTDT
                     WRALRIMGEFVEGFDALAAVGPAVSVFGSARIRRGDPSYRMARSLSRKLAKHGYAIIT
                     GGGPGIMEAANRGAREAGGVSVGCNIELPFEQGLNEFVTLGMEFRYFFVRKTMFVKYA
                     EAFCIFPGGFGTLDELFESLTLIQTGKVQHFPVVLFGSTYWSGLLDWLRANPLDEGKI
                     SPDDLT"
BASE COUNT          632 a         1311 c         1329 g          614 t
ORIGIN      
        1 ccgcttcgac ctgccgatcg tggcacccgg aagcgcgttc caggagcgga tctgggccga
       61 gctgcaacgg ctcgcggcgg ggacgacgct cagctacgag gagctcgcgc aacgcctcgg
      121 tcggccacgt acgcaacgag cggtgggtac cgccaacggg gcgaaccgga tcgcagtcgt
      181 gatcccgtgc catcgagtgg tgcgcaagac cggcgagacg ggcaactacg gcggcgggcg
      241 ctggcggaag gaatggctcc tggcccacga ggcggggctc gccccggctc cggcctagcg
      301 gcggatgtcg gtccccatgg cgtggtagcc accgtcgacg tggatcagct cgcctgaagt
      361 cttggcggcc cagtcgctga gcaggaagca gaccatctgc ccgaccgcgg tcgcatcgtt
      421 catgtcccat cccagcgggg cgcgcttgag ccacgtgtcg gcgatctggt cgaagccggg
      481 gatgctcttg ccggccatgg tccggatcgg gccggccgag acggtgttca cgcgaatgtg
      541 gtgctcaccc aggtcgcgcg ccaggtagcg ggtgaccgcc tcgaggcccg ccttggcgac
      601 ccccatccag tcgtagatcg gccaggcgaa gcgcccgtcg aaggtgaggc tcaccaccga
      661 cgcgctgccg gccgccttca tcggctcaag caggccaacg ctgagcgcct tgagcgaata
      721 ggccgatgtc tggaagccga acgccacgct ctcccagggg gtgttgagga agttcccgcc
      781 cagcccgtcg ctcggcatga agccgatggc gtggacgacc ccgtcgacct tgccccagcg
      841 tttcatcagc tccgcgctca gcgcctcgat gtgctccggg ttgttgacgt ccagctcgag
      901 cacgtccggc gttgtcggca gcttcttcgc aacccgctgg gtcaggctca tcccgcggcc
      961 gaccccggta aggagcacct cggccccggc ctcctgcgcg accttcgcaa tgctgaacgc
     1021 gatgctgtcg tccgtcagca cgccggtgat gagcagcttc ttgccgtcga gcaacatggc
     1081 ggcgattgta ccggccgttc catcaggcga tactcgccca ctgatgatgg tccacctcta
     1141 cgactacgag gttcccgtgg cggatgctgc caccgccatg ccctccctgc gcgaggcgct
     1201 gtcagctctg ggagacgagg atcgcaaggc gctccttggc gggctcttca agcaggagcg
     1261 catgccgggc accatggaac gcctctccac cctggtcagc gacctcgacg ccagcttcgg
     1321 cgacctgctg accctcctcg acaactcggc tcgcgctcgg cgcttcgtgg cgatcgacga
     1381 ggcgaaggac gtcctggacc gatacctccc ggccaacgcc gtctcgggca cgcatgactg
     1441 ggacgtagcc cgcctgcacg agccgtatgg cgacggcacc taccacggca agctgctgaa
     1501 gcgggtgcgc aagcagctgc gcagcgtggg cacgcgcgag gcactgacca cgccgttcct
     1561 cctccgtcgc gagtggggcc gctgaccgca tgagccgccg atcgcagcgc cgcggacgcc
     1621 cctaccagcc cctgccggcg cgccaggtga actggggcgc gaggctcgtg atcgtcttcg
     1681 tcgccttcgt cctggtcgct ggcttcgcga tcctcagctt cgctcgctga acggcggccc
     1741 gtttgcactc gctgcaaata acggccaacc acggacaatc cccgccgctc cacgaagcgg
     1801 atcgtgcggg agaagcggct tccacggcca ccgcgggctc ccagtggcca cgaaggctcg
     1861 agatcaggag cgaaattggc cgttgtttgc gcccggtgca aacgcattgc gcgattctcg
     1921 cgcttccgcg ctcagtcgat gacgagcggc ttgaccgtca tcacctcgtc gatgcgccct
     1981 tcccattcgg agcgtggcgc gtcgcagtac agctcgatct cattgccgtc cgggtccgcc
     2041 gtgtagaggg atcggctcac cccgtgatcc acggcaccgc gcaggagccc gcgctcggtg
     2101 agcagcgcat gggcggtccg caccgcgtcg aggtcggcga gctggattgc gacgtggtag
     2161 agcccgaccg ccgtgggaac cggcgacgcc gcctgctgtc ccactcgcag cagggccaga
     2221 tcgtggtggt tctgcccgtc cgcggcgagg aagatcatca cgttgctcag ctcgctgacc
     2281 accttgaagc caagcaggtc gcggtagaag gcgagggatc ggtcaaggtc gcgcaccttg
     2341 agcacgacgt gcccgatgcg aaggggccgc agcatcgccg gatgctacca gcggcgcgtg
     2401 gcggcgaggc gaaggatctc agccgccgag gtggacggtg acggtgatca ctccgcgcga
     2461 ggtgctgctg tcgctgatcg cggcccacgc ctcgggcgag aggtcggcga ccttctggtc
     2521 agcggtgccc cacaggcagc cgcaatagtc cgccaccgga agcaccgcgc agcgatcggc
     2581 gcagaccgtc acgaaacggg ccgcaggtgg ggcgtcgtaa tgaccgccga gcgcccccgg
     2641 cagcgccacg acggcctgac cgcgccagcc ggcggagcag caataccagg tcgcctcgcc
     2701 ggagaccgtg tcgccgctga ccggggccgc caccgacttc ggcgcgacct tcttggcggg
     2761 agctgggtag aggctcgcct ccatcgcaat ggttggcggg gtggccggcg ctggggcgag
     2821 gcccaggtcc tcggtgccga tcggctggct gacgcctgag agcgagagcg acgctgatcg
     2881 gttgcgaccg tcgagcgacg agcggtcagc caggctgagc ggctgatccg cgacagcgag
     2941 cagtcgcatc gactcagctg aggcgggggt cgccgatcgc gcgctgagcg gagccatgag
     3001 gccgatgacc accgcaaaga cgatggttcg cactgcgacg cgggccaaga ggtccggcaa
     3061 tagaatcctc ggactagcgg gttgagttcg ggtccgccga ggcgaaagcc gcacgatggc
     3121 tgacgcactt gcccgcgatt ggcacggtcg cgacccgcat gaatcgacgg ctcaccctac
     3181 catccgactt taggcgtgtc gagcaggaga tggtcatggc taccaagcgc aaccaggcgc
     3241 accgcagcgg ggccgtggtc atgcgcggcg acgagaagcg gcctaccgag gacgagcagc
     3301 tcctgcagca ggaaggcact gacgcctctt tcctgaagac cgacacctgg cgtgcacttc
     3361 gcatcatggg tgagttcgtc gagggcttcg atgccctcgc cgccgtcggt ccggcagtga
     3421 gcgtgttcgg gtcggcgcgg atccgacgcg gagatccctc gtaccgcatg gctcgctcgc
     3481 tctcacgcaa gctggccaag cacggctacg cgatcatcac ggggggcggt cccgggatca
     3541 tggaggctgc caaccgggga gcccgtgagg cgggtggcgt atcggtcggc tgcaatatcg
     3601 agctgccgtt cgagcaggga ctgaacgagt tcgtcacgct cgggatggaa tttcgctact
     3661 tcttcgtgcg caagacgatg ttcgtcaagt acgcggaggc gttctgcatc ttccccggcg
     3721 gcttcgggac cctcgacgag ctgttcgagt cgctgaccct gatccagacc ggcaaggtgc
     3781 agcacttccc ggtggtcctg ttcgggtcca cctattggag cggactgctc gattggctga
     3841 gggcgaaccc tctcgacgag ggcaagatca gcccggacga tttgac
//
LOCUS       DATCQR010000183        13525 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_180908, whole genome shotgun sequence.
ACCESSION   DATCQR010000183 DATCQR010000000
VERSION     DATCQR010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 13525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13525
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180908"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..765
                     /locus_tag="VIN32_06150"
     CDS             <1..765
                     /locus_tag="VIN32_06150"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HEY8922195.1"
                     /translation="TADDDAVWGYGLGCNGAIELFVEPTAGALESVGALRTGEGTCLV
                     IPLDGPSAGLHRLETDGAAAAALRDGSPRVEEVGGERIFYEPLLPPLRLLVCGAGHDA
                     IPLVRQAMELGWRVVVVDVREGLLTAQRFGVGVDFANPDPEAAADALAPDERTAAILM
                     SHNYLRDIAYLRSFLDRPLAYLGVLGPRGRTEQMLAEIGRPEAIAWLHAPAGLDIGAE
                     GPEEVAHSIIAEILAVLRQREGGLLRDRHGPIHDLD"
     gene            complement(762..4037)
                     /locus_tag="VIN32_06155"
     CDS             complement(762..4037)
                     /locus_tag="VIN32_06155"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY8922196.1"
                     /translation="MAERKLVSVLFADLVGFTSMSESRDAEAVREQLTEYFGVAQEIV
                     TRYGGAVEKFIGDAVMAVWGTPVAHEDDAERAVRAALELVDAVSHLGHDGEKLSARAG
                     VLTGEAAVTLGASNQGMVAGDLVNTASRLQSVAPPGTVIVGEATRQSAESSIVFEPVG
                     DQELKGKVSPVPAYRAVRVVAQRGGVGRADQLEAPFMGRDTEMRLVKDLFHAAPREKR
                     PRLLSIIGQAGIGKSRLAWEFLKYIDGITLNVLWHQGRSPAYGEGISFWALAEMVRSR
                     VGLLELDDPATTRAKLARALETYVPDDAERRWMAPRIEQLLGMADAEAGGRDDLFPAW
                     RTFFERVAGDNPAVLVFEDLHWADPGQLDFIDHMLEWSRGHAIYIITLARPELLERRP
                     NWGAGQRSFTSLDLEPLSDEVIREILASLVPGLPEPTIEQIVSRAEGIPLYAVETVRM
                     LLQDGRLEQDDGVYRPVGDLSTLAVPATLHALIASRLDALDATDRSLLQYASVLGQTF
                     TLKGLKAVTGQGDDLETRLHALVRRELLTLDSDPKSPERGQYGFVQALVRDVAYGTLT
                     KRDRKVVHLAAGEHFESLQDDEIIDATASHYLDAYRSAPEDPDAEGIRAHAAELLQRA
                     AVRATGLGSHEQAVRFLELALDVTPDPEHQSQLLRQTGDAAGAAGRYEQAVGYLRRAR
                     SAAQEAGHKPSEARALASLGRQLATGAQPAAAIEEITAALPALSGLEEDASIVSVWGA
                     LSRAYMLHSELAPAVEWADRALPLAERLDMVPEIADILNTRATSLGFGGRIRESVAGL
                     RGVLEMSSSYELPNAIFRARINLSSVLSLEDPVASWTIAAQGFEDAKRAGAVEAMATM
                     GGNAAEAALHVGEWDSAESILRELQEVELAPSDRMVVDSFGALSDVLRGRSSDAGLAR
                     LEAFAATSDEPVVIFQLSTTKGWIAFMEGRFADAHALYATALDALKGGISADEPMLAR
                     TALWAGMPEQARAAAEAQRQSGSHGRAINAAARATEAGVAALTGHSDEAVAAYRDAIR
                     QWRDLGCWFDLALCQLDFVKFVGGESPEVEAAATEARSTFTRLGAPALLERLDEAVGL
                     PNA"
     gene            complement(4536..5561)
                     /gene="moaA"
                     /locus_tag="VIN32_06160"
     CDS             complement(4536..5561)
                     /gene="moaA"
                     /locus_tag="VIN32_06160"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017885376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HEY8922197.1"
                     /translation="MPELLPMLPRDTRGRPLHDLRISVTDRCNFRCVYCMPRAVFGRD
                     HAFLPRAELLTFDEIARLVGILTRLGVQKVRLTGGEPLVRRELPTLVAMLAATPGVKD
                     LTLTTNGVLLPEHAPALREAGLHRVTVSLDADDDATFMRMNDAGVPVSRVLAGIEAAE
                     AAGLGPIKLNMVVKRGWNEHAVLPMARRFRGTGRILRFIEYMDVGHSNGWRLDEVISA
                     DEILSTISAEFPLEPMAPTKPGEVAERYRYADGAGEIGIIASVSRPFCGDCNRARLSA
                     DGQLYTCLFATSGHDLRGLLRGGATDADLEATVSAIWEARDDRYSEIRSAETVALPKV
                     EMSYIGG"
     gene            complement(5573..6241)
                     /locus_tag="VIN32_06165"
     CDS             complement(5573..6241)
                     /locus_tag="VIN32_06165"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEY8922198.1"
                     /translation="MSDRWATFDCYGTLIDWMSGIRGSLARLWPEADADSLLTLYHQL
                     EPAVQAGRGTAYRDVMAETLAEVCALARLEVPAGEEGALGASLPRWPVFAEVPVALAE
                     LRSRGWRLAILSNTDADLLDASLQAIGVPVDLRIVASEIGSYKPAVRHWEAFFHQTGA
                     ARARHVHVAASLFHDIEPGARLGLSCVWINRLAERSDVPRAAELNDLSALPETLEALV
                     PDRG"
     gene            6298..7686
                     /locus_tag="VIN32_06170"
     CDS             6298..7686
                     /locus_tag="VIN32_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013426732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY8922199.1"
                     /translation="MTPHDAFLDTLRAAVGGSHVLTDPDLRASYETDWTRRWRGEALA
                     VVRPATTDEVISVLLACAAAGAAVIPQGGNTGLVGGSVPRAVADAPQVVVSTLRLRDL
                     EPVDALGGEVMVGAGVTLAALQAHLRPAGFTFGVDLGARDSATIGGMIATNAGGIHVL
                     RHGPMRAQLLGLEAVLADGSVVRRLPGLVKDNTGYHLPSLMAGSEGTLAVITRAHLRV
                     VPIRPRRAVALLALAEVADAVGLAGDLRRSLPELAAAELFFDEGMELVMHHVTADRPF
                     RDRHPAYLLLEADGDDDPTDLLAGAVANAIGVDDAVIASDEAGRARLWNLRERHTEAI
                     SAEGVAHKLDVTLPLARLAEFIGRVPELVTSTRPGSVTYQYGHVCDGNMHVGIIGPAP
                     DDEAVDDAVLRLTIEMGGSVSAEHGIGAAKVAWLEADRGVADVSAMRAIKRALDPRWM
                     LNPGVLLREAEA"
     gene            complement(7683..8420)
                     /locus_tag="VIN32_06175"
     CDS             complement(7683..8420)
                     /locus_tag="VIN32_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015722687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEY8922200.1"
                     /translation="MDPTLILALLFLVAALLYGSVGHAGASAYLASMALLGVAPEVMK
                     PTALVLNILVASIVTLRFARAGYVRPWALLPFLLGSIPAAFIGGGLTLPTTIYRPLVG
                     AVLLVAAARFGWTASRAGEDFPPRAPWPAAAASGAGIGLLAGLTGTGGGIFLTPLLLA
                     AGWAGTRFAAGTSAAFILANSISGLAGNLASVGSLPPAIPLWLGAVAVGGAVGSQLGS
                     RRMPAPWIRRALAVVLLVAGLKLIFLP"
     gene            complement(8442..9797)
                     /locus_tag="VIN32_06180"
     CDS             complement(8442..9797)
                     /locus_tag="VIN32_06180"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY8922201.1"
                     /translation="MERFDLAIIGAGPAGEAAAFKARLLGASVAIIDRDLFGGACPFF
                     ACMPSKSLLHSAAVHAAGGDYPWPKASDRRDWMISREGIDYPDDTRRFTALQKAGAVP
                     MRGSARLDGPGRVVVNHGDVDHELEAGGVIVAVGSTAKVPELPGLADIPYWTNREATS
                     TRELPESLLILGAGPTGVELSQVYARYGVPVTLVVPSPRILPRDHERSSAAVARALAR
                     DGVTIHTGARAVAAHAGAGTNGRHRFDLDDGTTVEGAAVLLAIGRTLPLAGLGLESVG
                     VDAAAARLQPDAQLRIAPRVFVAGDPAGPEMHTHVSHYEGEMAARIALGEDVTPDFRA
                     LPRAIYTDPEAASVGLLVEQAKEGGIDAEELTSDLASTAKGEAAEAEGHVTIVVDRAA
                     SALAGVFMAGPGVSEAIHEAVLAVKLQTPLSVLADTIHAFPTVARVMGLLFAKAATTR
                     R"
     gene            complement(9849..11036)
                     /gene="hutI"
                     /locus_tag="VIN32_06185"
     CDS             complement(9849..11036)
                     /gene="hutI"
                     /locus_tag="VIN32_06185"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HEY8922202.1"
                     /translation="MPPESFLLTGRGRLLRPEPYDGTLPWAIVVRNGRIAWLGARADA
                     PDADTVSDLADALVTPGLVNGHTHPAFLGDRSDEAAARLAGAPYDGGGILRTVATTRA
                     ASDEELRLAIEGRLRAELAAGTTTVECKSGYGLSTAEELRALRLIGEAARSVSGHVVR
                     TFLGAHAVPPEALSMDAYATLVAEEMLPAVADAGAAEFCDVFCDRDFFSAEATERILR
                     VAASLGLGTRLHADQLTPSGGAELAARLGATSADHLEQLDAAGVAALAGSRTVATLLP
                     GPAIVMGGGLPPARALLDAGATVALASDANAGTFAGWGAMPLVIGLGATLLGMTVEEA
                     LTAATAGGAAALRLAGRKGVLQVGADADLVAWDAEHEGAFALRLGAVRPAQVFVSGRV
                     ATT"
     gene            complement(11054..11986)
                     /locus_tag="VIN32_06190"
     CDS             complement(11054..11986)
                     /locus_tag="VIN32_06190"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY8922203.1"
                     /translation="MIAYPFGALARRPVMTAVLGAMSIAFSGILVRLAHVEPATAAIF
                     RCAYALPALGLIAYLERRAHGPRPAAQVRLAMLAGVFLALDLVIWHHTIGLVGAGLAT
                     VLGNTQVVIVAVLAWWILRERPPRRTLLALPVVLFGVVLISGVIGSGAYGANPGLGVI
                     LGLVVGFVYAAFLLVLRRGNADLRRPAGPLFEATLVGAVASLTIGLPLGEVNLVPSWP
                     AHGWLLLLALTSQVVGWLIISVSLPRLPAALTSVILTLQPVGSVLLAMLLLGETPSVV
                     QLLGAGTILVGLVLATLRFGGEPRRQPIAEPEIG"
     gene            12065..>13525
                     /locus_tag="VIN32_06195"
     CDS             12065..>13525
                     /locus_tag="VIN32_06195"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY8922204.1"
                     /translation="MTEIPTGTVTFLFTDIEGSTRLAQDLGDAWPPLLERHRQIARAA
                     WAEQRGVEIGTEGDSFFVVFTSAPQAVAAAVAAQRGLAAEAWPADGQVRSRMGLHTGE
                     GVLSGGSYVGLDVHRAARIRSAGHGGQVLLSATTRALVEGALPDGVTLREMGEHRLKD
                     LSRPERIWDLVIDGVRSDFPALSTLNAVPNNLPMQLTSFLGRQREIAEGRQLLIDGRL
                     LTLTGPGGTGKTRLSLQIAADATDRFPDGIYFVPLGTISQSDLVLPTIAQAVGLVDPG
                     PQALDRLVEHIGAKCFLLVLDNFEQVNDAAPQIAELLVRAPKISVLVTSRSPLRVSGE
                     REYPVPPLGVPDPRHLPDLEQFSTYESVALFVERAMAVRPDFAVTSANAPAVAEICVR
                     LDGLPLAIELAAARVRVLTPQAIMDRLGDRLKLLSGGSRDLPERQQTLRGAIAWSHDL
                     LDEADQRVFARFAVFAGGATLDAVERVVFDADDSADP"
BASE COUNT         2065 a         4700 c         4827 g         1933 t
ORIGIN      
        1 accgccgacg acgacgcggt gtggggctac gggcttggct gcaacggggc gatcgagctc
       61 ttcgtcgagc cgaccgccgg tgccctcgaa tcggttggcg cgctgcgcac cggagagggc
      121 acgtgcctcg tcatcccgct cgacggccca tccgcgggcc tacaccgcct tgagaccgat
      181 ggcgccgctg ccgcggcgct gcgtgacggc agcccgcgcg tcgaggaggt cggcggcgag
      241 cggatcttct acgagccgct gctgccgccg ctgcgcctgc tcgtgtgcgg cgccgggcac
      301 gatgccatcc cgctggtgcg acaggcgatg gagcttggct ggcgggtggt cgtggtcgat
      361 gtccgcgaag ggctgctcac tgcgcagcgc ttcggggttg gggtcgactt cgccaatccg
      421 gacccggagg cggctgccga cgcgcttgcc ccggacgagc ggaccgcggc catcctgatg
      481 agccacaact acctgcggga catcgcctac ctgcgttcct tcctggaccg gccgcttgca
      541 tatctgggcg tgctcggtcc ccgcggccgc accgagcaga tgctggccga gattggtcgg
      601 ccggaggcga tcgcctggct ccacgcaccg gcgggcctgg acatcggggc ggaggggccg
      661 gaggaggtgg cgcactcgat catcgcggag atcctggctg tcctccgcca gcgcgaaggc
      721 ggactgcttc gggatcggca tggtccgatc cacgatctgg actaggcgtt cggcagcccg
      781 acagcctcgt ccaggcgctc caggagagcg ggtgctccca atcgggtgaa ggtggaccgg
      841 gcctcggttg cggcggcctc aacttcggga ctctcgccgc ctacgaattt cacgaagtcc
      901 agttggcaga gggcaaggtc gaaccagcac ccgagatcgc gccattggcg gatggcatcg
      961 cgataggcag ctaccgcctc atccgagtgg ccggtgagcg ccgcgacgcc cgcctccgtg
     1021 gcacgcgccg ccgcattgat ggcgcgacca tgtgatccag attggcgctg cgcttccgct
     1081 gcggctcgcg cctgctccgg catgcccgcc cacagggcag tccgcgcgag catcggctca
     1141 tcggctgaga tgccgccctt gagcgcgtcg agggcagtcg cgtacaacgc atgggcatcg
     1201 gcgaaccgac cctccatgaa cgcaatccat cccttcgttg tgctcaattg aaagatcact
     1261 accggctcgt cggaggttgc cgcgaaggcc tcgaggcgag ccagcccagc gtcagacgag
     1321 cgaccgcgaa gcacgtctga gagtgccccg aaggaatcga ccaccattcg atccgatgga
     1381 gcgagctcga cctcctgcag ctcccggagg atggattccg cgctatccca ctcgcccacg
     1441 tggagcgctg cctctgccgc attgccgccc atcgtcgcca tggcctcgac cgcgccggct
     1501 cgcttcgcgt cctcgaagcc ctgcgccgca attgtccagc tagcgacggg gtcttccagc
     1561 gagagcacgc tgctgaggtt gatgcgggcc cgaaagatgg cattggggag ctcatacgac
     1621 gagctcatct cgagcacgcc gcgaaggccg gcaaccgatt cccgaatacg tccaccgaag
     1681 ccgagagagg tcgcccgcgt attcaggata tccgctatct ccggcaccat gtccagccgc
     1741 tccgccagcg gcaacgcgcg atccgcccac tccaccgcag gcgcaagttc gctgtgcagc
     1801 atgtacgcgc gcgagagggc accccacacc gagacgatgc tggcgtcctc ctccagcccc
     1861 gacagcgccg gaagggcggc agtgatctct tcgatcgcgg cggctggctg cgcgccggtg
     1921 gccagctggc ggccgaggct ggcgagggcg cgggcctcgc tgggcttgtg ccctgcctcc
     1981 tgcgcagcgg atctcgcccg acgcagatag cccacggcct gctcgtacct cccggcggcg
     2041 cccgctgcat ccccggtctg gcgaagcagc tgcgactggt gctcgggatc gggtgtcacg
     2101 tccagcgcca gctcgaggaa gcggaccgcc tgctcgtgcg agccgagccc cgtggctcgc
     2161 acggccgcgc gctggagcag ctccgcggca tgcgcccgaa tcccttcagc gtcggggtcc
     2221 tcaggcgcgc tgcggtatgc gtcgaggtag tgcgaggcag tggcgtcgat gatctcgtca
     2281 tcctggagcg actcgaagtg ctcgccggcc gcaaggtgca ccaccttgcg atcacgcttc
     2341 gtgagcgtgc cataagccac gtcccggacc agcgcctgca cgaagccgta ctggccgcgc
     2401 tccggcgact tgggatcgga gtcgagcgtg agcagctcac gccgaaccag cgcgtgcagg
     2461 cgcgtttcga gatcgtcccc ctggccggtg accgctttca ggcccttgag cgtgaaggtc
     2521 tggccaagga ccgatgcgta ctggagcagg gaacgatcgg tggcgtcaag ggcgtccagc
     2581 ctggaagcga tcagggcgtg cagcgtcgcc ggaaccgcca gcgtgctcag gtcgcccaca
     2641 ggccgataga cgccgtcgtc ctgctcgagc cggccgtcct gcaacagcat ccgcaccgtc
     2701 tcgacggcgt agagcgggat tccctcggcg cgcgacacga tctgttcgat cgtcggttct
     2761 ggcaggccgg gtaccaggct ggccaggatc tcccggatca cctcgtcgga gaggggttcg
     2821 aggtcgaggc tggtgaagct gcgctgcccc gcgccccagt tgggacgacg ctcgagcagc
     2881 tcggggcgcg ccagcgtgat gatgtagatc gcgtgaccac ggctccattc gagcatgtgg
     2941 tcgatgaagt cgagctggcc gggatcggcc cagtggaggt cctcgaagac aaggaccgcg
     3001 ggattgtcgc ccgcgacgcg ctcgaagaag gtccgccacg cgggaaagag gtcgtcgcgc
     3061 cccccggcct cggcatcggc catgcccagc agctgctcaa tgcggggtgc catccagcgc
     3121 cgctcggcgt catcgggcac ataggtctca agcgccctgg cgagcttggc gcgcgtggtg
     3181 gcggggtcgt ccagctcgag caggccgacc ctgctgcgca ccatctccgc gagggcccag
     3241 aagctgatcc cctcgccgta ggccggcgag cggccctgat gccagagcac gttcagcgtg
     3301 atgccgtcga tgtacttcag gaactcccag gccagccggc tcttcccgat cccggcctgg
     3361 ccgatgatcg agagcagccg cgggcgcttt tcgcgaggcg ccgcgtggaa gaggtccttc
     3421 acgaggcgca tctcggtgtc gcgccccatg aagggggcct cgagctggtc ggctcggccg
     3481 acaccgccac gctgggccac cacccgcaca gcacgatagg ccggcaccgg actgaccttg
     3541 cccttcagct cctgatcgcc gacgggctcg aagacgatgg agctctcggc gctctgtcgg
     3601 gtcgcctctc cgacgatcac cgttccgggc ggcgccaccg actggaggcg cgaggccgtg
     3661 ttgaccaggt ctccagcgac catcccctgg ttcgacgccc ccagcgtgac ggccgcctca
     3721 ccggtcagga cgccggcgcg ggcgctgagc ttctcgccat cgtgaccgag gtggctgacc
     3781 gcgtccacca gctccagtgc tgcacgcacg gcccgctccg cgtcgtcttc gtgcgccacg
     3841 ggggtgcccc ataccgccat caccgcgtcg ccgatgaact tctcgacggc ccctccgtat
     3901 cgcgtcacga tctcctgcgc gaccccgaag tactcggtca gctgctcgcg cacggcctcg
     3961 gcatcgcggg actcggacat cgaggtgaag ccgaccaggt cggcgaagag gacggagacg
     4021 agcttgcgct cggccaccgt gctcgcggag ccagcgtcgg gtgcaatagc gggagacacc
     4081 gcgggctgcg gtgcgacggc ggagccattg ttcgttgccg aggtctgcgt tgccggcgcg
     4141 cctccaagtc caaatccgca ctccggacag aatttcgggc tcccctcgta gggcgtcgag
     4201 cacgatgggc acgcctgttg caggcgagtg ccgcattcgg cacagaactt ctggttcggg
     4261 gacgcggcag cgccgcatga cgggcagagc attgggccga caggataccg gcgaccggcg
     4321 gcggtgccta gcgccgtttg cgcccagcgc aaacaacggg caacttcgca tcgcggatct
     4381 gccaccgaag ccccctcgcg acgattccgc tgcgcctgct tggtcgccgt ttgccctcag
     4441 tgcaaacctg ggcgaactga cggtaggtcg agggtctgct tggtccttat ttgcgcggtg
     4501 tgcaaatgca cggggcgacg aaggccgaaa agggcctagc cgccgatgta gctcatctcc
     4561 accttgggca gggccaccgt ctcggctgag cggatctcgg agtagcggtc gtcgcgggcc
     4621 tcccagatcg cactgaccgt ggcttcgaga tccgcatcgg tcgcgccgcc gcgcaggagc
     4681 ccccgcagat cgtggccgct ggtcgcgaag aggcaggtgt acagctgccc gtcggcggag
     4741 agtcgggcgc gattgcagtc gccgcagaac ggccggctga ccgacgcgat gatcccgatc
     4801 tcgccggccc catccgcgta gcggtagcgc tccgccacct cgccgggctt cgtgggggcc
     4861 atcggctcaa ggggaaattc cgcgctgatg gtcgacagga tctcgtcagc cgagatgacc
     4921 tcgtccaggc gccagccgtt ggagtgcccg acgtccatgt actcgatgaa acgcaggatg
     4981 cggccggtcc cccggaagcg gcgggccatg ggcagcaccg cgtgctcgtt ccaaccacgc
     5041 ttcacgacca tgttcagctt gatcgggccg aggccggccg cctccgcggc ctcgatcccg
     5101 gccagcactc gcgagaccgg cacgccggcg tcgttcatgc gcatgaacgt ggcgtcgtca
     5161 tcggcgtcga ggctgacggt gacccgatgc agccctgcct ccctcagcgc gggcgcgtgc
     5221 tcggggagca ataccccgtt ggtggtcagc gtgaggtcct tgacgccggg cgttgcggcc
     5281 agcatcgcca ccagcgttgg cagctcgcgg cggacgagtg gctcgccacc ggtcaggcgc
     5341 accttctgca cgccgagacg ggtgaggatc ccgaccagcc gggcgatctc gtcgaaggtg
     5401 agcagctctg cgcggggaag gaaggcgtgg tcgcggccaa agaccgcccg aggcatgcag
     5461 tagacgcatc ggaagttgca gcggtcagtg acgctgatgc ggagatcgtg gagcggccgt
     5521 ccgcgggtgt cccggggcag catggggagg agctcgggca tgggcgcaga tcctaccccc
     5581 ggtcggggac cagcgcctcc agggtttcgg gcagggcgct gaggtcgttc agctcagcgg
     5641 cccgcggcac gtcgcttcgc tcggccaggc ggttgatcca cacgcagctc aagccgagcc
     5701 gtgcgcccgg ttcgatgtcg tggaagagcg aggctgccac gtgcacgtgg cgcgcgcgag
     5761 cggcgccggt ctggtggaag aaggcctccc agtgacgtac ggccggcttg taggagccga
     5821 tctcggaggc cacgattcgc aggtcgaccg gcacgccgat tgcctggagc gaagcatcga
     5881 gcaggtcggc gtcggtgttc gagaggatgg cgagtcgcca gccacggctc cgcagctccg
     5941 cgagcgccac gggcacctcg gcgaagaccg gccaccgggg cagcgacgcg cccagcgcgc
     6001 cctcctctcc ggccggcacc tccaggcggg cgagcgcgca gacctcggcg agggtctcgg
     6061 ccatgacgtc gcggtacgcc gtgccgcggc ccgcctggac cgccggctcg agctggtggt
     6121 agagggtcag gagggaatcg gcgtccgcct cgggccagag gcgggcaagc gagccccgga
     6181 tgccggacat ccagtcgatg agcgttccgt agcagtcgaa cgtggcccat cggtcgctca
     6241 tcgggcgagt ctacgagcca gaccggaacg ccagcaggcg tggcaggatg ttcgccgatg
     6301 acgccccacg acgccttcct cgacacgctg cgcgccgcgg tcgggggcag ccacgtcctg
     6361 accgatcctg acctgcgcgc ctcgtacgag acggactgga cccgccgttg gcgtggggag
     6421 gcgcttgccg tcgtgcgtcc cgcaacgacc gacgaggtga tctcggtgct gctggcctgc
     6481 gcagccgccg gcgcggcggt gattccgcag ggtggcaata ccggcctggt gggcggctcc
     6541 gtgccgcgtg ccgtggccga tgccccgcag gtcgtcgtct ccaccctgcg cttgcgggac
     6601 ctcgagcccg tggacgccct ggggggtgaa gtgatggttg gtgcgggcgt gacactcgcc
     6661 gcactgcagg ctcatctgcg tcccgccgga ttcaccttcg gggtcgacct gggggcaagg
     6721 gactcggcga ccatcggcgg catgatcgcc accaacgcgg gcggaatcca cgtgctgcgt
     6781 catgggccga tgcgcgcgca gctgcttggc ctggaagcgg tcctggccga tggaagcgtg
     6841 gtgcgccgcc tgccgggatt ggtcaaggac aacactggct accaccttcc ctcgctcatg
     6901 gcgggcagcg agggaaccct ggccgtgatc acgcgcgcgc acctgcgcgt cgttccgatc
     6961 aggccgcggc gcgcggtcgc cctgctggcc ctggcagagg tggccgatgc agttgggctc
     7021 gccggagacc tgcgccgatc cctgccggag cttgccgcgg ccgagctctt cttcgacgag
     7081 gggatggagc tcgtcatgca ccacgtcacg gccgatcggc cgtttcgcga ccgccacccg
     7141 gcctacctgc tcctcgaggc cgatggtgac gacgacccga cggacctgct tgccggcgcc
     7201 gtcgcgaacg ccatcggtgt cgacgacgcg gtcatcgcgt ccgacgaggc cggccgcgcc
     7261 aggctgtgga acctccggga acggcacacc gaggcgatct ccgccgaggg ggtcgcgcac
     7321 aagctcgacg tgacgctgcc gctcgcgcgc ctcgccgaat tcatcgggcg cgtgccggag
     7381 ctggtgacat cgaccaggcc gggctccgtg acctaccagt acggacacgt gtgcgacggg
     7441 aacatgcacg tggggatcat cggcccggcg cctgatgacg aggcggtgga cgatgccgtc
     7501 ctgcggctca cgatcgagat gggagggtcg gtgagtgccg agcatgggat cggcgccgcc
     7561 aaggtggcct ggctggaggc agatcgagga gttgccgacg tctccgccat gcgggccatc
     7621 aagcgcgcgc tcgacccgcg ctggatgctg aatccgggcg tcctgctgcg cgaagccgag
     7681 gcctagggca ggaagatcag cttcagcccg gcgaccagca ggacaactgc caacgcgcgg
     7741 cggatccagg gggccggcat gcgccggctg ccgagctgcg acccgacagc cccgccgaca
     7801 gccacggctc ccagccagag cgggatggcc gggggcaggc tgccgaccga cgccaggttg
     7861 cccgccaggc ccgagatgga gttggccagg atgaacgcgg ccgaggtgcc tgccgcgaat
     7921 cgcgtccccg cccaccccgc ggcgaggagc agcggcgtca ggaagatgcc gccgcccgtt
     7981 ccggtcaggc ccgcgagcag gccgatccca gcgccggagg cggccgctgc aggccagggg
     8041 gcccgcggtg ggaagtcttc acccgcgcga gaggcggtcc acccgaagcg tgcggctgcc
     8101 accaggagga cggcgccgac cagtggccga tagatggtgg tcggcagcgt gagcccgccg
     8161 ccgatgaagg cagcggggat cgagccgagc aggaatggga ggagcgccca gggtcggaca
     8221 tagcccgcgc gagcgaagcg cagggtcacg atgctggcca ccaggatgtt gaggaccagg
     8281 gcggtcggct tcatgacctc cggcgcgacc ccgagcagcg ccatgctggc gaggtaggcg
     8341 gaggcgccgg cgtgcccgac ggacccgtac aggagcgcgg caaccaggaa gagaagagcc
     8401 aggatcaggg tcggatccac gccgcgatcc tacgggctac gctagcggcg cgtggtggct
     8461 gccttggcga acaggaggcc catcacccgg gccaccgtgg ggaaggcgtg gatcgtatcc
     8521 gccaggacgg agagcggcgt ctgcagcttc acggccagga ccgcctcgtg gattgcctcg
     8581 ctgacccccg gtccggccat gaagacgccg gcgagggcgc tcgccgcgcg atcgaccacg
     8641 atcgtgacgt ggccctccgc ctcggccgcc tcgcccttgg cggtcgacgc cagatcgctg
     8701 gtcagctcct cggcgtcgat gccaccctcc ttcgcctgct cgaccagcag gccgaccgag
     8761 gcggcctccg gatcggtgta gatggcgcgc gggagggcgc ggaagtccgg cgtgacgtcc
     8821 tcacccagtg caatccgcgc cgccatctcg ccttcgtagt ggctgacgtg ggtgtgcatc
     8881 tccgggcccg ccgggtcgcc ggccacgaag acccggggcg cgatgcgcag ctgtgcatct
     8941 ggctgcagcc tggcggccgc ggcgtcgacg ccaacgctct ccagccccag tccggccagt
     9001 ggcaatgtgc ggcctatggc gagcaggacc gccgcgccct cgacggtcgt gccatcgtcg
     9061 aggtcgaacc gatgccgccc attcgtgccc gcccccgcat gcgccgcgac cgcgcgcgcc
     9121 cccgtgtgga tggtgacccc gtcgcgcgcg agagccctgg cgaccgcggc cgagctgcgt
     9181 tcgtggtcac gcggcaggat ccgcgggctg ggcacgacca gggtgaccgg gacgccatac
     9241 cgcgcgtaga cctgcgacag ctccacgccc gtggggccgg cacccaggat cagcaggctc
     9301 tccggcagct cccgggtcga ggtggcttcg cggttcgtcc agtacgggat gtccgccagg
     9361 cccggcagct ctggcacttt ggcggtggag ccgacagcga cgatcacgcc tccggcctcg
     9421 agctcgtggt cgacatcgcc gtggttgacg accacccggc ccggcccgtc gaggcgtgcg
     9481 ctgccccgca tcggcaccgc gccggccttc tgcagggcag tgaagcggcg ggtgtcgtcg
     9541 gggtagtcga tcccttcgcg gctgatcatc caatcgcgac gatccgacgc ctttggccac
     9601 gggtagtcgc cgccggcggc atgcaccgcc gccgaatgga gcagggactt ggagggcatg
     9661 cacgcgaaga acgggcaggc gccgccgaac aggtcgcgat ctatgatcgc caccgaggcg
     9721 ccgagcaagc gcgccttgaa tgccgcggcc tcgcccgccg ggccggcccc gatgatcgcc
     9781 aggtcgaatc gttccattgg cgcagcatca tagggcggcg gtcgcgcgat ccggcgcgag
     9841 gcgccgtcct aggtggtcgc gacccgaccc gagacgaata cctgcgcggg acgcaccgcc
     9901 ccaaggcgca gggcgaaggc accttcatgc tcggcgtccc atgccacgag gtcggcgtcg
     9961 gcgccgacct gtagcacccc ttttcgcccg gcgagccgca gggctgctgc gcctccagcc
    10021 gtcgcagcag tgagggcctc ctccacggtc atcccgagca gcgttgcgcc caggccgatc
    10081 acaaggggca ttgcacccca acccgcgaag gtgccggcgt tggcgtcgct ggcaagggcg
    10141 acggtcgcgc cggcgtcgag gagggcacgc gccggaggaa ggccgccgcc catgacgatc
    10201 gcggggcccg gtagcagcgt cgccacggtc ctcgaaccgg ccagggcagc cacgccggcg
    10261 gcatccagct gctcgaggtg atccgcactg gtcgcgccga gccgcgctgc cagctcagct
    10321 ccaccggagg gggtcagctg gtccgcgtgc aggcgcgtcc cgaggcccag gctggcagcc
    10381 acgcggagaa ttcgctcggt ggcttcagcg gagaagaagt cacgatcgca gaagacgtcg
    10441 cagaattcgg cggctccagc gtctgccacg gcggggagca tctcctcggc aaccagcgtg
    10501 gcgtacgcgt ccatggagag ggcctcgggc gggacggcat gggcaccaag gaaggtgcgc
    10561 acgacgtggc cggagacgga ccgagccgcc tcgccgatca ggcgcagcgc ccggagctcc
    10621 tccgccgtcg agaggccgta gccggacttg cactcgaccg tggtggtgcc ggcagccagc
    10681 tcggcgcgca gccggccctc gatcgcaagg cgcagctcct cgtcggaagc cgctcgagtg
    10741 gtcgccaccg tgcgcaggat cccgccgccg tcgtatgggg ctcccgccag ccgggcggcg
    10801 gcttcatcgg accggtctcc aaggaatgcc gggtgcgtat gtccgttgac gagtcccggg
    10861 gtcaccagtg catcggcgag gtcggagacg gtgtcggcat ccggcgcgtc ggccctggcg
    10921 cccagccacg cgatccggcc attgcgcacc acgatcgccc acggcagggt tccgtcgtat
    10981 ggctcgggcc tgaggagccg gccgcgcccg gtgagcagga acgattccgg aggcatcggg
    11041 ccaaccatag cccctagccg atctctggct cggcgatcgg ctggcgtcgt ggctcgcccc
    11101 caaagcggag ggtggcaagc accagcccga ccaggatcgt gccagcgccc agcagctgga
    11161 ccaccgatgg cgtctcgccc agcaggagca tcgccagtag caccgagccg accggctgga
    11221 gggtcaggat gacgctggtc agggcggcgg gcaggcgcgg gagcgagacg ctgatgatca
    11281 gccacccaac gacctgtgag gtgagggcca gcagcagcag ccagccgtgc gccggccagc
    11341 tcgggacgag gttgacctcg cccagcggca gcccgattgt gagcgacgcg acggcgccca
    11401 ccagggtggc ctcgaagagt gggccggccg ggcgccgcag gtcggcattg ccccgtcgca
    11461 gcacgagcag gaaggcggcg tacacgaatc ccaccacgag gccgaggatc acgccgagcc
    11521 ccgggttggc gccgtacgcc cccgaaccga tgacgcccga gatgagcacg acgccgaaca
    11581 ggaccaccgg cagggcaagc agcgtgcgtc gaggcgggcg ctcgcgcagg atccaccagg
    11641 ccaggaccgc cacgatgacc acctgcgtgt tgccgagcac cgtggccagc cccgcgccga
    11701 cgaggccgat ggtgtggtgc cagataacga ggtccagagc caggaagacg ccggccagca
    11761 tcgcgagccg gacctgggcc gccggccgcg gaccgtgggc gcgtcgctcg agatacgcga
    11821 tcagtcccag cgcgggcagg gcgtacgcgc agcgaaagat ggccgcggtc gccggctcga
    11881 cgtgcgcgag tcgcaccagg atgccggaga aggcgatcga catcgccccg agcaccgccg
    11941 tcattacggg ccgtcgagca agggcgccga agggatacgc gatcatcgcc gggcagggta
    12001 gccgagcgat gtggcttgcg ctggatggct tgccgtcagt ggagcggccg acgacacttg
    12061 tctcgtgacc gagattccga ccgggacagt taccttcctg ttcaccgaca tcgagggctc
    12121 gacgcgcctt gcccaggacc tgggcgacgc gtggccacct ctgctggaac ggcatcgcca
    12181 gatcgcccga gcggcctggg cggagcagcg cggcgtcgag atcgggacgg agggcgactc
    12241 cttcttcgtg gtcttcacca gcgctccgca ggcggtcgcg gccgcggtcg cggcgcagcg
    12301 cggcctggcg gcggaggcat ggcccgcgga cgggcaggtt cgcagccgga tggggctgca
    12361 caccggcgag ggcgtgctga gcggcggctc atacgtgggg ctcgacgtgc atcgcgcggc
    12421 acggatccgc agcgcggggc acggtggcca ggtactcctg tcggcaacca cccgagcgct
    12481 ggtggagggc gcgctgcccg atggggtcac actgcgcgag atgggggagc atcggctcaa
    12541 ggacctctca cgaccggaga ggatctggga cctggtgatc gacggagtgc gcagcgactt
    12601 tccggcgctg agcaccctga acgcggttcc caacaacctg ccgatgcagc tgacctcgtt
    12661 cctcgggcgc cagcgcgaga tcgcagaggg ccggcagctg ctgatcgacg gtcggctgct
    12721 gaccctgacc ggcccgggcg gaacggggaa gacccgcctc tcgctccaga tcgccgccga
    12781 cgcgaccgat cggtttcccg acggcatcta cttcgtgccg ctcggcacca tcagccagtc
    12841 cgacctggtg ctgcccacga tcgcgcaggc ggtcggcctg gtggacccgg ggccccaggc
    12901 cctcgatcgc ctggtcgagc acatcggcgc caagtgcttc cttctcgtcc ttgacaactt
    12961 cgaacaggtc aacgacgcgg cgccgcagat cgccgagctc ctagtgcgcg cgccgaagat
    13021 ctcggtgctg gtcaccagcc gcagtccgct gcgcgtctcc ggcgagcgcg agtacccggt
    13081 cccgcccctc ggcgtccctg atccgcggca tctgcccgac ctggagcagt tttccacgta
    13141 cgagtcggtg gcgctcttcg tcgagcgcgc catggccgtg cgtcccgatt tcgcggtaac
    13201 cagcgcgaat gccccggcgg tggccgagat ctgcgtgcgc ctcgatggac tgccgctggc
    13261 gatcgagctg gccgcggcac gggttcgagt cctcacgccg caggcgatca tggatcggct
    13321 gggagaccgg ctgaagctcc tctcgggcgg gtcgcgtgac ctcccggaac gccagcagac
    13381 gctccgcggc gcgatcgcct ggagccacga tctcctggat gaggccgacc agcgtgtctt
    13441 cgcccgcttc gccgtcttcg ccgggggagc caccctggat gccgtcgagc gggtcgtctt
    13501 cgacgccgat gattccgcgg atccg
//
LOCUS       DATCQR010000184         3216 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_103012, whole genome shotgun sequence.
ACCESSION   DATCQR010000184 DATCQR010000000
VERSION     DATCQR010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3216
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103012"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(86..1117)
                     /locus_tag="VIN32_06200"
     CDS             complement(86..1117)
                     /locus_tag="VIN32_06200"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018086079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEY8922205.1"
                     /translation="MPEMTYSEALNLALREEMRRDPAVFVMGEDVAVWGDRGGVFGVT
                     RGLAEEFGPERVRDTPINEEAIVGVAVGAALTGMRPVVEIMYSDFLTLTMEPLVNQAA
                     KLRYMFGGQARVPLVVRTNLGASGGKAAQHSQSLETWMMHVPGLKVAMPSTPADARAL
                     LRTAIRDDNPVVFFEHKLLYFQRGEVPEQQEELVFGRAAVRREGRHATVVAAQAMLGK
                     VLAVAGRLASEGINLEVIDPRTLVPFDIETVVESVRKTNRLLICHEATERGGWAAEVA
                     MQVGELAFDHLDAPVARVCGANVPIPYSTPLETAVVPGEAAIEKGLRVLLAGATVDSS
                     RDRPSTLTS"
     gene            complement(1110..2027)
                     /locus_tag="VIN32_06205"
     CDS             complement(1110..2027)
                     /locus_tag="VIN32_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEY8922206.1"
                     /translation="MMLIRAFDSLLPALYAKELIRGSSHAAIGQEAVAVGACAALAPD
                     DYITSTHRGHGHTIAKGGDVNRMMAELMGREDGYCRGKGGSMHIADFSIGMLGANGIV
                     GGGFGIAAGAALSAQMRRSGQVALCFFGDGAINQSSFHGVANLAGIWKLPLILLCEDN
                     RFAMSGRAELMIAGGDPVKRAQAYGFPGVSVDGMDVLAVKAAVAEARARACAGDGPTL
                     VVAACYRFQGHFSGDTQSYRTKEEMAPWLERDPIPAFRSRLLAEGGLSEAEVDAMEEE
                     VATAIAEALEFAQASPWPEAQEAWEDVDA"
     gene            complement(2053..2802)
                     /locus_tag="VIN32_06210"
     CDS             complement(2053..2802)
                     /locus_tag="VIN32_06210"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY8922207.1"
                     /translation="MALLDRIALVTGAGSERGLGNAIARALARAGAEVILSDIDVDGA
                     RRNAAAIGARARALALDVTSEESVAEAVREAGRVDVLVNNAGITQRRAIWELSIDEFD
                     RMLAINLRGGFLCLSAVVKGMMERRWGRVIWISSVAGKQGGGIIGTAHYAASKAAVIG
                     LCQAAARELGPYGITSNAIAPGFILTGIATRAVDAQAEREMDEKISAEAPMRRSGLPD
                     DVAAAAAFLASEEAGYITGEVMDVNGGIHFD"
     gene            3013..>3216
                     /locus_tag="VIN32_06215"
     CDS             3013..>3216
                     /locus_tag="VIN32_06215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8922208.1"
                     /translation="MLFFFVYWALRRVLELIVVLGRRGPANEIELLVLRHEVVVLRRQ
                     LPRPQLWPSDRALFGALAQVLPRD"
BASE COUNT          518 a         1163 c         1009 g          526 t
ORIGIN      
        1 gctttaatct gcctcagccc caagtggcac cgcacaactt cgcggactcg cccatcaggg
       61 ttcgcagtgg gatttgcggt agacgtcagc tggtgagcgt cgatggccga tctctgctgg
      121 aatccacggt ggcacccgcc aagagcacac gtaggccttt ctcgatagca gcctcaccgg
      181 gcacgacggc cgtctcgagc ggcgtgctgt acggaattgg cacgttggca ccgcagaccc
      241 gtgccacggg tgcgtcgagg tggtcgaagg cgagttcgcc cacctgcatc gcgacctccg
      301 ccgcccagcc gccgcgctcg gtcgcctcgt ggcagatgag cagccggttg gtcttgcgca
      361 ccgactccac caccgtctcg atgtcgaagg gcaccagcgt gcgggggtcg ataacctcga
      421 ggttgatgcc ctccgacgcc agccttccgg ccaccgccag caccttgccg agcatggcct
      481 gggcggcgac gacggtggcg tggcgacctt cccgccgaac cgccgcccgg ccgaacacaa
      541 gctcctcctg ctgctcgggg acctccccgc gttggaagta gagcagcttg tgctcgaaga
      601 agacgaccgg gttatcgtct cgaatcgccg tcctgagcag cgcacgggcg tcggccggcg
      661 tactcggcat cgccaccttg aggccgggga cgtgcatcat ccaggtctcc agcgactgcg
      721 agtgctgggc ggccttgccg cccgacgcac ccaggttggt gcgcacgacc agcggcaccc
      781 gggcctgacc gccgaacatg tagcggagct tggccgcctg gttgaccagc ggctccatgg
      841 tcagggtcag aaagtcggag tacatgatct cgaccaccgg ccgcatgccg gtgagcgccg
      901 cacccaccgc cacgccgacg atcgcctctt cgttgatcgg ggtgtcgcgg acacgctccg
      961 gcccgaactc ttcggccagc ccgcgggtca ccccgaagac gccgccgcgg tcaccccaca
     1021 ctgcaacgtc ctcacccatg acgaagaccg caggatcgcg ccgcatctcc tcgcgcaggg
     1081 ctagattgag tgcttccgaa tacgtcatct caggcatcga cgtcttccca ggcctcctgc
     1141 gcctccggcc aggggctggc ctgtgcgaac tccagcgcct cggcaatcgc cgtcgccacc
     1201 tcctcctcca tggcgtcgac ctcggcctca ctcagcccgc cctcggcgag caggcgggaa
     1261 cggaaggcgg ggatcgggtc gcgctccagc cagggagcca tctcctcttt ggtgcggtag
     1321 ctctgcgtgt cccccgagaa gtgcccctgg aagcgatagc aggcggcaac caccagcgtc
     1381 gggccatctc cggcgcacgc ccgcgcgcga gcctcggcca cggcggcctt gaccgccagt
     1441 acgtccatgc cgtccaccga gacaccgggg aagccatagg cctgcgcccg cttgaccggg
     1501 tcgccacccg cgatcatcag ctccgcccgg cccgacatcg cgaagcggtt gtcctcgcac
     1561 agcaggatca gcggcagctt ccagatgccg gccaggttgg ccactccatg gaacgagctc
     1621 tggttgatcg cgccgtcacc gaagaagcag agcgccacct gaccggagcg acgcatctgc
     1681 gcgctgagcg cagctccggc cgcgatgccg aacccgccgc ccacgatgcc gttggccccc
     1741 agcatcccga tcgaaaaatc ggcgatgtgc atcgaaccgc ccttgccgcg gcagtagccg
     1801 tcctcccggc ccatcaactc ggccatcatc cggttgacgt cgccgccctt ggcaatcgtg
     1861 tggccgtggc cccgatgggt gctggtgatg taatcgtccg gagccagcgc agcgcaggcc
     1921 ccaacagcca ccgcctcctg cccgatcgcg gcgtggctgg aaccgcggat cagttccttg
     1981 gcgtagaggg caggcaggag ggagtcgaag gcgcgaatca gcatcattcg gcgcagcatc
     2041 tcaacgctgg tgtcagtcga agtggatgcc gccattgacg tccatcacct cccctgtgat
     2101 gtagcccgcc tcctcggagg ccagaaaggc cgctgcggcg gcgacgtcat cgggcaagcc
     2161 gctacggcgc atcggtgcct ccgcgctgat cttctcatcc atctcgcgct cggcctgcgc
     2221 gtcgaccgcc cgggtcgcga tgccggtgag gatgaagccg ggggcgatcg cgttgctcgt
     2281 gatcccatac ggcccgagct ctcttgcagc cgcctggcag aggccgatga cggcggcctt
     2341 gctggccgcg taatgggcgg tgccgatgat ccccccgcct tgtttgccgg ccacgcttga
     2401 gatccagatc acccgacccc agcgacgctc catcatgccc tttacgacag cactcaggca
     2461 caggaacccg ccccgcagat tgatggccag catccgatcg aactcgtcaa tcgagagctc
     2521 ccagatcgcc cgtcgctgag tgatgccggc attgttcacg aggacgtcca cgcggcccgc
     2581 ctctctcacg gcttcggcca ccgactcctc gctggtcaca tcgagggcta gcgcgcgggc
     2641 gcgcgcgccg atcgccgccg cattgcgacg cgccccgtca acgtcgatgt cggagagaat
     2701 gacctccgca cccgccctgg ccagcgcccg cgcaatcgcg ttgcccaggc cccgctccga
     2761 gccggcgccg gtaaccaacg cgatgcgatc caggagcgcc acctcagcag cctagcggct
     2821 cggcgcgaga ctgacggtcg gcgaataatc tcagaagagt cattacgggc cgatacccgc
     2881 tatatcccgt gaaagtgttc cctatctgga cgagccagca gcctacgcag tcgtgtgggt
     2941 gccgagaact cggaacgcgg gcgattcgta gcggtaggct ccgatctcgg ccgtgtccgg
     3001 caggatgagt tcgtgctttt cttcttcgtc tactgggcgc tgcgccgtgt ccttgagcta
     3061 atcgtcgtac tcggtcggcg tggaccggcc aacgagatcg aactgctggt cctccgccac
     3121 gaggtcgtgg tcctgcggcg gcagctgccc cgtccacaat tgtggccgtc cgaccgggcg
     3181 ctgttcggcg cgttggccca ggtgctgccc cgggac
//
LOCUS       DATCQR010000185        11404 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_103031, whole genome shotgun sequence.
ACCESSION   DATCQR010000185 DATCQR010000000
VERSION     DATCQR010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 11404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11404
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103031"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1020)
                     /locus_tag="VIN32_06220"
     CDS             complement(<1..1020)
                     /locus_tag="VIN32_06220"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY8922209.1"
                     /translation="MAQTADAIIVGAGVQGASLAFHLAERGMRPLVLERATVAAGATG
                     RSSGLVRMHYDVEAEARLAWRSHAYFCDWEKRVGGESGFTVVGFLQMVPPDEIPALLA
                     NTAMHQRIGIRADLVDADEMRRILPGIEIAEDQVAAFEPLSGYADPTQTTSSLLAAAV
                     AREARLIQGCAVTGIRTDVGQVIGVDTNRGPFDAPIVVDAAGAWAGRIAAMVGLEVPI
                     TVWRHDVAYVQRPPDVPRHVAFIDFENAFYCRPEGESLSLVALEDGNPLTGDPDATVD
                     AAAPGFIEKAAERLVRRLPDFARAGMHSAHSGQDGITPDQHPIIGPAGPDGFWLDCGF
                     SGTGFK"
     gene            1103..2038
                     /locus_tag="VIN32_06225"
     CDS             1103..2038
                     /locus_tag="VIN32_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014375365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8922210.1"
                     /translation="MPPTDEILVYARGLTKRFVEFTAVNGVDFDVRTAEAFGFLGPNG
                     AGKTSTMRMIACSSPVSEGELTVIGMDPRTQAAQIKARLGVVPQADNLDAELTVEENL
                     LMYARYFDIPMSVARPRAAELLEFMQLTERARDQVEPLSGGMKRRLTIARALINDPEL
                     IILDEPTTGLDPQARHLLWERLYRLKQRGATLIITTHYMDEAEQLCDRLVVMDKAAIV
                     AAGTPRELIEKYATKEVLELRIPDDARGSLDGRLDGLAERIEELPDRFLLYADDGEKA
                     LEGVHGREIPIDSALVRRATLEDVFLRLTGRSLVE"
     gene            2038..2868
                     /locus_tag="VIN32_06230"
     CDS             2038..2868
                     /locus_tag="VIN32_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014375366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8922211.1"
                     /translation="MAVLTDSRPSPTPPANALTGAIAGAARILQRNLLVYRRTWRGSL
                     FGSFLTPLLYLTAMGIGLGQLMARGGSDLLGGVPYLDFLGPGILASSCMQTASFESTY
                     PIMGKISWRRNYEAILATPIEVHHLLIGELSWIAFRLTTMATVFMIVLTLFGIPRSPL
                     ALLAIPAAVLTGVAFAAPIIAFSATRHNDSGFAALFRFIINPLFLFSGTFFPVSRLPD
                     AVEWVAAATPLYHGVALVRGAVLDNMPSTWPLHVTYLVLFIAITGYIAYRLLRRRLVK
                     "
     gene            2865..3689
                     /locus_tag="VIN32_06235"
     CDS             2865..3689
                     /locus_tag="VIN32_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020385506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8922212.1"
                     /translation="MTAIARRAPAPGPANGLVASLLRSRRLVQRNLLVYKRGWMVIFS
                     GFFEPLFYLLGIGYGLGTLVGGVQLSDGRTITYAAFVAPALLATASMNGAIAESIFNV
                     FFKLNFSKTYDAILATPLGIREIAVGEMLWSLFRGTLYVIAFVLVMIGMGLVISPLLF
                     LVIPASILIGASFSAACLAASAYLRTVQDFDLPMGLVVMPMFLFSGTFFPISLYPQPI
                     QLAMELTPLFHAVGLLRGLATGLLGWHELWDLAYLVVFGAIAMWIALNRLEQRLIK"
     gene            3764..4213
                     /locus_tag="VIN32_06240"
     CDS             3764..4213
                     /locus_tag="VIN32_06240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8922213.1"
                     /translation="MTLTSEDPQPIRLILEDADLTGWVYTHGQRMTDLLQNGEPFSFL
                     PAGGDASQWIETFPDETLLVVPPPHVSEPTLRVHGHQTQVQIRAGAYRIAGTAHMRPG
                     EQDDPIMRATRRFLPLTSATFGRDEGPDEQVDVLIVNMRRTSEFHVA"
     gene            complement(4268..4873)
                     /gene="pgsA"
                     /locus_tag="VIN32_06245"
     CDS             complement(4268..4873)
                     /gene="pgsA"
                     /locus_tag="VIN32_06245"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEY8922214.1"
                     /translation="MTDSRPPSRLTTLPNMLGLARIAATPIVVLLLLLPFPAAGLIGF
                     VVYAAAATTDYLDGRIARARDQVSPLGVFLDLTADKVLVAGVLVAMVEVNILPTWIVA
                     TLLIRELVVQGVRQVAAEASVVIAARQLGKWKAFATNAAIGVLLLAFDAETGGPVGKL
                     IDAGAVHAIGFWLAILATVLAVVSGVAYLRGALPMLLGREG"
     gene            complement(4870..5496)
                     /gene="plsY"
                     /locus_tag="VIN32_06250"
     CDS             complement(4870..5496)
                     /gene="plsY"
                     /locus_tag="VIN32_06250"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEY8922215.1"
                     /translation="MTGIPGAAVLVLLGYLIGALPFGLLVGRLAGGVDLREHGSGRIG
                     ATNALRTVGLAGAVLTFLLDVGKGVVAVLMVGWLYVAGPPGSPLWVAAAVGIAAVAGH
                     IRSVFIGFRGGRGVATFAGTMLATVPWVAAIVLPLGALIVWRSRIVSLGSVSGALLAP
                     VVTAALALVGQATWAAVALALGGSVLVTIAHRDNIARLRAGTERRLGQ"
     gene            complement(5525..5863)
                     /locus_tag="VIN32_06255"
     CDS             complement(5525..5863)
                     /locus_tag="VIN32_06255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8922216.1"
                     /translation="MSREERRAYQRMTKKQDPYAIPATGAARARQQQLERRKAQRAAT
                     VSAPPGAISRRFLAWAIGGALVSGLVAFSLAWPSGMPFAAYVGAGVAIAWVAVAFGAR
                     ALGRRNPVRP"
     gene            5908..8592
                     /gene="aceE"
                     /locus_tag="VIN32_06260"
     CDS             5908..8592
                     /gene="aceE"
                     /locus_tag="VIN32_06260"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HEY8922217.1"
                     /translation="MHEIFDQFKQQLPDIDPAETQEWLDSLDGVVSQAGPERARFIIY
                     KLLKRARQLQVGLPPLVQTRYVNTISTEQEPNFPGDEKMEHRIRQMIRWNALAMVLRA
                     NSSFEGIGGHLSTYASAATLYEVGFNHFFRGRDDGAAGDQIFYQGHAAPGIYARAFLE
                     GRLSEEQLDHFRRETGGPGTGLASYPHPRTMPDFWQFPTVSMGLGPISAVYQARFNRY
                     LAARGISDTSASRVWAFVGDGEMDEPESLGALGLAAREGLDNLIFVVNCNLQRLDGPV
                     RGNGKIIQELEATFRGAGWHVIKVIWAREWDDLLARDRDGVLLTKMNETLDGDYQKLS
                     VADGAYIREHFFGPDPRLRALVEHLPDEALAKLRRGGHDYRKVYAAYAAAVEHTGAPV
                     VILAKTVKGWTLGPGIEGRNVTHQAKKLNQEELKVFRDRLELPIPDEQLKEAPYYHPG
                     PESPEIAYMLERRHHLGGLVPRRVVQAKPLPAAADAAYAELFAGADRPASTTMAFSRL
                     LRNLVRDPQLGKRIVPIIPDEARTFGMDPLFKELGIYNPLGQRYTAVDKELLLSYVEK
                     TDGQILEEGITEAGAMADFTAAATSYATHGEPMIPFYTFYSMFGFQRTGDQIWAMGDA
                     RGRGFMMGATAGRTTLTGEGLQHDDGHSHILASTVPNVLAYDPAFAYELAIIVREGIR
                     RMYGEPGEDVFYYLTLYNENWAMPPKPDGVEEGVLRGLYRFKAAERGSRRVQLLGSGS
                     ILHQALRAQEVLAERYDVAADVWSVPSFQQLRNDALSAERWNRLHPEAEARVPYVVQQ
                     LGQTKGPIIAATDYLKSLPDMVARWIDRPFTVLGTDGFGRSDTREALRTHFEVSPEQI
                     AYAALHGLCLTGSADRRELVKAIGELGIDPDRTDPREA"
     gene            complement(8596..9096)
                     /locus_tag="VIN32_06265"
     CDS             complement(8596..9096)
                     /locus_tag="VIN32_06265"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEY8922218.1"
                     /translation="MDLRLLPYQPDRDVYRLLGVLPSAGRAEIVDACRRLARAFHPDR
                     NASGRATEEMQVVNAVRSLLTDPRSRAEYDYARLRFLQPSAGASPAPANAQRRWRPAL
                     MPASAVGLGHAARAAMFALVAAMRALPRARCDDCNAWVEPEYRYCPACGARLRGATPL
                     IAGGRG"
     gene            9304..9459
                     /locus_tag="VIN32_06270"
     CDS             9304..9459
                     /locus_tag="VIN32_06270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8922219.1"
                     /translation="MYNQFQLMIVNTENEIRGVRSRKTVRSHVATGDNGIKRVFRLNP
                     DRQGGRR"
     gene            9460..9900
                     /locus_tag="VIN32_06275"
     CDS             9460..9900
                     /locus_tag="VIN32_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011194309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2089 domain-containing protein"
                     /protein_id="HEY8922220.1"
                     /translation="MARDVISTCPVCEGELAITRLHCRSCGTALEGEFGVGRFGRLSR
                     EQLTLLESFLRSRGNLKDLERELGISYPTVRGRIEALLRALGLADGDEAAPEEPAEEP
                     AEADAPNPSRDDDAAKRRAILDLLARHEMSAEHAAEALRALGGA"
     gene            9904..10878
                     /locus_tag="VIN32_06280"
     CDS             9904..10878
                     /locus_tag="VIN32_06280"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HEY8922221.1"
                     /translation="MGTFVRTQSIDHSVGERGRVSLKVTSSDVQVRGIPGDEAHIRAT
                     FKISASSDADADRIFEAVQLRVNRGSGELSVEEHEGNYSVGSVIGRLFGANGHYELAV
                     EAEVPTAAEVQLAAVSSDVDVTDLHGEQRYQTVSGDLSVSNGGGSVRLQSVSGDATIR
                     AEEPLSIQSQGVSGDLNVTAPILRSLRASTVSGDVELEAQLDEGGDFRVETVSGDLSV
                     GLVGDATFEIRGLSTDVSSELDHRLEGQVDRRRLIVGNGGARLVFNSMSGDVDVRRPR
                     RLSAEPRRTEPVPEPTTEEKQSELDVLRALERGEIDVDEATRRLAGGR"
     gene            10879..11256
                     /locus_tag="VIN32_06285"
     CDS             10879..11256
                     /locus_tag="VIN32_06285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8922222.1"
                     /translation="MGDELATVLRLVAEGKLSADEAAPIIEALGRHSPPRPPEPPAAV
                     PPLTAGKARGRRVRIQVTERGRRVVDVRVPLAFAAMAARMVPGIPDSYAALIERAVED
                     DMVGPIVDLAEDEDGDGVLITVE"
BASE COUNT         1890 a         4042 c         3773 g         1699 t
ORIGIN      
        1 cttgaagccc gtgcccgaga agccgcaatc gagccagaaa ccatcgggtc ctgccggacc
       61 gatgatcggg tgctggtcgg gagtgatccc atcctgcccg gaatgagccg agtgcattcc
      121 ggcgcgcgcg aagtcgggca ggcgccgcac cagccgctcg gctgccttct cgatgaaccc
      181 gggcgcagcg gcatcgaccg ttgcatcggg atcaccggtc agcggattcc cgtcctccag
      241 tgcgacgagg ctgagcgact ccccctcggg gcggcaatag aaggcgttct cgaagtcgat
      301 aaaggcgacg tggcgcggca cgtccggcgg gcgctgcacg taggcgacgt catgacgcca
      361 cacagtgatc ggcacctcga gcccgaccat cgccgcgatg cggccggccc atgcccccgc
      421 ggcgtcgacg acgatcggcg cgtcgaacgg acctcggttc gtgtcgacgc cgatcacctg
      481 gccgacatcg gtccggatcc cggtcacggc gcagccctgg atcagtcgcg cctcgcgggc
      541 caccgccgcg gccagcaggg agctggtggt ctgggtcgga tcggcgtagc cggagagcgg
      601 ctcgaaggcc gccacctggt cctcggcgat ctcgatcccc ggcagtatgc ggcgcatctc
      661 atcggcgtcc actaggtcgg cccggatgcc gatgcgctgg tgcatggcgg tgttcgccag
      721 cagcgccggg atctcgtcag gcggcaccat ctggaggaag ccgaccaccg tgaatcccga
      781 ctcgccgccg acccgcttct cccagtcgca gaagtaggcg tgcgaacgcc acgcgaggcg
      841 cgcctcggct tcgacgtcgt agtgcatccg caccaggccg ctcgagcgac cggtcgcgcc
      901 agccgccacg gtcgcgcgct cgagcaccaa cgggcgcatc ccccgctccg ccaggtggaa
      961 ggccagcgag gctccctgga cgcccgcccc gacgatgatc gcgtctgccg tctgcgccat
     1021 ggcgaggacg gtagtggcgc tggctaaggc gccaccagtg ccgaatggct cctctccgac
     1081 gcatcggtag aatggtccgc ccatgccccc gaccgacgag atccttgtct atgcccgcgg
     1141 actgacgaag cgattcgtcg agttcaccgc ggtgaacggc gttgacttcg acgtgcgcac
     1201 ggctgaggca ttcggattcc ttggtcccaa cggcgcgggc aagaccagca ccatgcgcat
     1261 gatcgcgtgc tcctcgccgg tgagcgaggg ggagctgacg gtcatcggca tggacccgcg
     1321 cacgcaggcg gcgcagatca aggcacgcct gggcgtcgtc ccgcaggccg acaacctcga
     1381 cgccgagctg accgtcgagg agaacctgct gatgtacgcg cgctacttcg acatcccaat
     1441 gtcggtggcg cgcccgcggg cagccgagct gcttgagttc atgcagctca ccgagcgggc
     1501 ccgcgaccag gtcgaaccac tttccggggg gatgaagcgg cggctcacca tcgcccgggc
     1561 actcatcaac gacccggagc tgatcatcct ggacgagccg acgacgggcc tcgacccgca
     1621 ggcgcgccac ctcctgtggg agcgcctcta ccggctcaag cagcgcggcg ccacgctcat
     1681 catcaccacg cactacatgg acgaggccga gcagctgtgc gaccgcctgg tggtgatgga
     1741 caaggccgcg atcgtcgcag caggcacacc cagggagctg atcgagaagt acgcgaccaa
     1801 agaagtgctt gagctgcgca tccccgacga tgcccgcggc agcctcgatg gccgcctgga
     1861 tgggctcgcc gaacgaatcg aggagctgcc agatcggttc ctcctctacg ccgacgacgg
     1921 cgagaaggcg ctcgagggcg tgcacggccg cgagatcccg atcgactcgg cgctcgtccg
     1981 gcgtgccacg ctcgaggacg tcttcctgcg gctcaccggc cgctccctgg tggagtgatg
     2041 gcggtcctca cggacagccg ccccagccca acgccgcccg cgaatgccct gacgggcgcg
     2101 atcgcgggtg cggcacgcat cctgcaacgc aacctgctgg tctatcggcg cacctggcgc
     2161 ggcagcctct tcggctcgtt cctcaccccg ctgctgtacc tgacggcgat ggggatcggc
     2221 ctcggccagc tcatggcccg cggtggctcc gacctgctgg gcggcgtccc gtacctcgac
     2281 ttcctgggcc ccggcatcct ggccagcagc tgcatgcaga ccgccagctt cgaatcgacc
     2341 tacccgatca tgggcaagat cagctggcgc cggaactacg aggcgatcct ggccacgccg
     2401 atcgaggtcc accacctgct catcggcgag ctgagctgga tcgccttccg gctgaccacg
     2461 atggctaccg tcttcatgat cgtgctcacc ctgttcggga tcccgcgcag cccgctggcg
     2521 ctgctggcca tccccgccgc ggtgctcacc ggggtcgcct tcgcggcgcc gatcatcgcc
     2581 ttctccgcca cgcgccacaa cgactccggc ttcgcggcgc tcttccgctt catcatcaat
     2641 cccctcttcc tgttcagcgg caccttcttc ccggtctccc ggctgcccga tgcggtggag
     2701 tgggtcgccg ccgccacgcc cctctaccac ggggtcgcgc tggtgcgtgg cgcggtcctc
     2761 gacaacatgc cgtcaacctg gccgctgcac gttacctacc tcgtcctctt catcgccatc
     2821 accgggtaca tcgcctaccg cctgttgcgc cggaggctgg tcaagtgacc gccatagcac
     2881 gacgagctcc ggcaccgggc cccgcgaacg gcctggtcgc ctcgctgctc cgatcgcgcc
     2941 gcctggtcca gcgcaacctg ctcgtctaca agcgcggctg gatggtcatc ttcagcggct
     3001 tcttcgagcc gctcttctac ctgttgggga tcggctacgg cctcggcacg ctggtgggag
     3061 gcgtccagct gtccgacggt cgtacgatca cctatgccgc gttcgttgcg ccggcgctgc
     3121 tcgccacggc gagcatgaac ggggcgatcg ccgagtcgat cttcaacgtc ttcttcaagc
     3181 tcaacttcag caagacctac gacgcgatcc tggccacgcc gcttgggatc cgcgagatcg
     3241 cggtgggcga gatgctctgg tcgctcttcc gcgggacgct gtacgtgatc gccttcgtgc
     3301 tggtcatgat cggcatgggc ctggtcattt cgcccctcct cttcctggtg atccccgcct
     3361 cgatcctcat cggcgcctcc ttcagcgcag cctgccttgc cgcctctgcc tacctgcgca
     3421 cggtgcagga cttcgacctg ccgatgggcc tggtggtgat gccgatgttc ctgttcagcg
     3481 gcacgttctt cccgatcagc ctttacccgc agccgatcca gctggcgatg gagctcacgc
     3541 cgctcttcca cgcagtgggc ctgctgcgcg gcctggccac cgggctgctg ggctggcatg
     3601 agctgtggga cctggcttac ctggtcgtct tcggtgcgat cgcgatgtgg atcgcgctca
     3661 atcgcctgga gcagcggctg atcaagtagc tgcccgtgag tcctgacggc tgtgaccggt
     3721 tggtaccgga ccttcggctc cgctccggga gtaaggtctg cccatgacct tgacaagtga
     3781 ggatcctcag ccgatccgtc tcatcctcga ggacgcggac ctcaccggat gggtctacac
     3841 ccacgggcag cgaatgaccg atctcctgca gaacggagag cccttctcgt tccttcccgc
     3901 gggcggcgac gcgagccaat ggatcgagac ctttcccgac gagaccttgc tggtcgtgcc
     3961 gcccccgcat gtcagcgagc cgaccctccg cgtgcacggg caccagacgc aggtccagat
     4021 ccgggccggc gcctaccgca tcgccggcac cgcccacatg cgcccgggcg agcaggacga
     4081 tccgatcatg cgagcgacac gacggttcct gccgctgacg tccgcgactt tcggccggga
     4141 cgagggtccg gatgagcaag tggacgtgtt gatcgtgaac atgcggcgga ccagcgagtt
     4201 ccacgtcgcc tgaccggtaa accagccggc ccttggcagc agggaactgc cgccgaccgt
     4261 gctcccttca gccttcccgc ccgagcagca tcggaagggc accgcgcagg taggcaaccc
     4321 ccgaaaccac cgccaggacg gtcgccagga tcgcaagcca gaagccgatg gcgtggacgg
     4381 caccggcgtc gatcagcttc cccaccggcc cgccggtctc cgcatcgaag gccagcagca
     4441 gcaccccgat cgccgcgttc gtggcgaacg ccttccactt gcccagctga cgggccgcga
     4501 tgacgacact cgcctccgct gccacctgcc ggaccccctg gaccacgagc tcccggatca
     4561 gcagggtcgc cacgatccag gtcggcagga tattcacctc gaccatcgcc accaggacac
     4621 cggcgacgag caccttgtcc gcggtgagat cgaggaacac gccgagcggc gacacctggt
     4681 cgcgtgcgcg ggcaatccgc ccgtcgaggt agtcggtggt ggccgccgcg gcgtagacca
     4741 cgaagccgat cagccccgca gcggggaacg gcaggaggag gagcaggacc acgatcgggg
     4801 tggcggcgat gcgcgccagg ccgagcatat tcggcagcgt ggtgaggcgc gacggcgggc
     4861 ggctgtcggt cattggccca gcctgcgttc ggtcccggcc cgcaaccgcg cgatgttgtc
     4921 ccggtgcgca atggtcacca ggaccgaacc gcccagggcg agcgcaaccg ccgcccaggt
     4981 ggcctggccc acgagtgcca acgccgccgt gacgaccggc gccagcagcg cgccgctcac
     5041 cgagccgagc gagacgatcc gcgaccgcca gacgatcagg gcgccgagtg gcagcacgat
     5101 cgcagcgacc cagggaacgg ttgcgagcat ggtgccggca aaggtcgcga caccgcgccc
     5161 tccccggaag ccgatgaaga ccgaccggat gtgaccggcc acggccgcga tcccgaccgc
     5221 cgccgcaacc catagcggcg agccaggcgg accggcgaca tacagccatc ccaccatcag
     5281 gacggcgacg actcccttgc cgacgtcgag caggaaggtc agcacggccc cggccagtcc
     5341 caccgtgcgc agcgcattcg tggcgccaat gcgcccggat ccgtgctcgc gcagatcgac
     5401 gccgcccgcc agccggccga ccagcagccc gaatggcagc gcgccgatga gatagccgag
     5461 caggaccagg accgccgccc caggaatgcc ggtcatcgtc gatcccccgc tcgtcgcacc
     5521 ggcgctaggg ccgaacgggg ttgcggcggc cgagtgcccg cgcgccgaag gcgacggcaa
     5581 cccacgcgat ggccacgccc gcgccgacgt acgccgcgaa cggcatcccg ctcggccagg
     5641 ccagggagaa ggcgacgagg ccgctcacca gggcgccgcc aatggcccag gcgaggaatc
     5701 ggcgcgaaat ggcgcccgga ggcgcgctga cggtggcagc acgctgcgcc ttgcgacgct
     5761 ccagctgttg ctgacgtgcc cgagcagcac ccgtcgccgg gatggcatac ggatcctgct
     5821 tcttggtcat gcgctgatag gcgcgccgct cttcgcgaga catcggctga gtctagtcgg
     5881 tcatccggtc tgtaccataa gaacccgatg cacgagatct tcgaccagtt caagcagcag
     5941 ctgcccgata tcgaccctgc cgagacgcag gagtggctcg actccctcga cggggtcgtt
     6001 tcgcaggcgg gtcccgagcg cgcccgattc atcatctaca agctgctcaa gcgcgcccgc
     6061 cagctccagg tcggcctgcc gccgctggtc cagacccgct atgtcaacac catctcgacc
     6121 gagcaggagc cgaacttccc cggcgacgag aagatggagc accggatccg gcagatgatc
     6181 cgatggaacg cgctggccat ggtgctgcgc gcgaactcga gcttcgaggg aatcggcggc
     6241 cacctctcga cctacgccag cgctgccacc ctgtacgagg tcggcttcaa ccacttcttc
     6301 cgcggccggg acgatggcgc ggccggcgac cagatcttct accagggaca cgcggcgccg
     6361 ggaatctacg cccgcgcctt cctggagggg cgtctgagcg aggagcagct cgaccacttc
     6421 cgtcgcgaga cgggcggccc gggaaccggc ctggcctcgt acccgcatcc gcgcaccatg
     6481 cccgacttct ggcagttccc caccgtcagc atgggcctcg gcccgatcag cgccgtctac
     6541 caggcccgat tcaaccgcta cctcgccgca cgaggcatct cggacaccag cgccagccgg
     6601 gtgtgggcct tcgtcggcga cggggagatg gacgagccgg agtcgctcgg agcgctgggc
     6661 ctcgccgcgc gcgagggcct cgacaacctg atcttcgtcg tcaactgcaa cctgcagcgg
     6721 ctcgacgggc cggtgcgcgg caacggcaag atcatccagg agctggaggc gaccttccgt
     6781 ggcgccggtt ggcacgtgat caaggtcatc tgggcgcgcg agtgggacga cctgctggcg
     6841 cgcgaccgtg acggcgtgct gctgaccaag atgaacgaga cgctcgacgg cgactaccag
     6901 aagctctcgg tcgccgacgg cgcctatatc cgcgagcact tcttcggccc agatccacgc
     6961 ctcagggcgc tggtcgagca ccttcccgat gaggcactgg caaagcttcg gcgcggtggc
     7021 cacgactacc gcaaggtgta cgccgcctat gcggcagcgg tggagcacac cggagcgccg
     7081 gtggtgatcc tggccaagac ggtcaagggc tggacgctcg gcccggggat cgaggggcgc
     7141 aacgtcacgc accaggccaa gaagctgaac caggaggagc tcaaggtctt ccgcgatcgc
     7201 ctcgagctgc cgatccctga tgagcagctc aaggaggcgc cctactacca cccggggccg
     7261 gagtcgcctg agatcgccta catgctcgag cggcgtcacc acctgggcgg tctcgttccg
     7321 cgccgcgtgg tacaggccaa gccgctcccc gcggcagcgg acgccgccta cgccgagctg
     7381 ttcgcggggg ccgaccgtcc ggcgagcacg accatggcgt tcagccgcct gttgcgcaac
     7441 ctcgtccgcg acccgcagct cggcaagcgg atcgtgccga tcatccccga cgaggcgcgc
     7501 acattcggca tggatcccct cttcaaggag ctcggcatct acaacccact cggccagcgc
     7561 tacacggcgg tcgacaagga gctcctcctc tcgtacgtcg agaagaccga cgggcagatc
     7621 ctggaggagg ggatcaccga ggccggcgcg atggccgact tcaccgccgc ggcgaccagc
     7681 tacgccaccc atggcgagcc gatgatcccg ttctacacct tctattcaat gttcggcttc
     7741 cagcgcaccg gcgaccagat ctgggcgatg ggcgacgccc gcggacgcgg gttcatgatg
     7801 ggcgccaccg ccggccgcac cacgctcacc ggcgaggggc tgcagcacga cgacggacac
     7861 agccacatcc tggccagcac cgtccccaac gtgctcgcct acgacccggc ctttgcctac
     7921 gagctggcga tcatcgtgcg cgaggggatc cggcgcatgt acggcgagcc gggcgaggac
     7981 gtcttctact acctgaccct ctacaacgag aactgggcga tgccgcccaa gcccgatggc
     8041 gtcgaggagg gcgtgctgcg cggcctctat cgcttcaagg ccgccgagcg agggtcacgg
     8101 cgcgtgcagt tgctcggatc aggctcgatc ctgcaccagg cactccgggc gcaggaagtt
     8161 ctcgccgagc gatacgacgt ggcagccgac gtgtggagcg tcccgtcgtt ccagcagctg
     8221 cgcaacgacg ccctctccgc cgagcgctgg aaccggctgc accctgaggc cgaggcgcgc
     8281 gtcccgtacg ttgtgcagca gctgggccag accaagggac cgatcatcgc cgccaccgac
     8341 tacctcaagt cgctgcccga catggtggcg cgctggatcg atcggccctt cacggtgctg
     8401 gggaccgatg gcttcggccg cagcgatacg cgcgaagcgc tgcgcacgca cttcgaggtg
     8461 agccccgagc agatcgccta cgccgcgctc cacgggctgt gcctgactgg gtccgcagac
     8521 cgcagggaac tcgtgaaggc tatcggcgag ttggggatcg atcccgaccg aacggacccg
     8581 cgcgaggcgt agccgctatc cgcgcccgcc ggcgatgagc ggcgttgctc cgcgcaggcg
     8641 cgcgccgcag gcggggcagt agcggtactc cggctcaacc caggcgttgc agtcgtcaca
     8701 tcgggcccgt ggcagcgcgc gcatggctgc cacgagagcg aacatcgccg cgcgcgccgc
     8761 gtgtcccagg ccgaccgcac tcgccggcat gagcgctggt cgccatcgac gttgcgcgtt
     8821 cgccggtgcc ggagaagcgc cggcgctcgg ctgcagaaag cgaagccgcg cgtagtcgta
     8881 ttcggcgcgt gatcgcgggt cggtgagcag cgatcgcacc gcattcacga cctgcatctc
     8941 ctcggtcgcg cggccggagg cgttgcgatc ggggtggaag gctcgagcca gccggcggca
     9001 ggcgtccacg atttcggcgc gaccggcgct cggaagcact cccagcagcc ggtagacgtc
     9061 gcgatccggt tggtacggca ggagacgcag gtccatcggg gctgggcgga tggtagccga
     9121 cctctgccgg tggtcgacgg ctgctcctgc acgctcgatt tgctaggagc ggccacatat
     9181 tgataagtcg tcatgccaaa atgcaagatc gcgcttgaca attcacaagc acgtggctaa
     9241 tgtgccgcta gcgactacaa gaacaagcaa ccgacctacc aaaacgcaag gagcgaagca
     9301 acaatgtaca accagtttca actgatgatc gtcaacaccg agaacgagat ccgcggcgtc
     9361 cgctctcgca agacggttcg ctcgcacgtg gccaccggcg acaacggaat caagcgcgtc
     9421 tttcgcctga accctgaccg ccagggcggg cgccggtaga tggcacgcga cgtcatctcc
     9481 acctgtcccg tctgcgaggg cgagctggcc atcacccgcc tgcactgccg cagctgcggg
     9541 accgcgctgg aaggcgagtt cggggtcggc cgcttcggtc gcctgtcacg tgagcagctc
     9601 acactgctcg agtccttcct tcgctcccgc ggcaacctga aggacctcga gcgcgagctc
     9661 ggcatctcct accccaccgt acgcggccgg atcgaagccc tcctgcgcgc gcttggcctg
     9721 gccgatggcg acgaggcagc acccgaggag cctgccgagg agcctgccga agccgatgcg
     9781 ccgaacccat ccagggacga cgatgcggcc aagcgccgcg cgatccttga tctcctcgcc
     9841 cgccacgaga tgtcggccga gcacgccgcc gaggcgctcc gcgcccttgg gggtgcgtag
     9901 gccatgggca ccttcgtccg cacccagtcg atcgaccaca gcgtcggaga gcgcggcagg
     9961 gtctcgctga aggtcacctc gagcgacgtc caggtccgcg gcattcccgg agacgaggct
    10021 cacattcgag ccaccttcaa gatctctgcc tcgtccgatg ccgatgcgga tcgcatcttc
    10081 gaggccgtgc agctgcgcgt gaatcgcggc agcggcgagc tgagcgtcga ggagcatgaa
    10141 ggcaactatt ccgtcggctc cgtgatcggc cgcctcttcg gcgcgaacgg tcattacgag
    10201 ctcgcggtcg aggccgaggt gccaaccgcc gccgaggtcc agctggccgc cgtctcctcc
    10261 gacgtcgatg tgacggacct gcacggtgag cagcggtacc agaccgtttc gggcgacctg
    10321 agcgtgagca atggcggagg gtcggtgcgc ctccagagcg tgtccggcga tgcgaccatt
    10381 cgcgcggaag agcctctgtc gatccagtca cagggtgtct cgggggacct gaacgtcacg
    10441 gcgccaatcc tgcgttccct gcgcgcaagc accgtgtccg gggacgtcga gctggaggcg
    10501 cagctcgacg agggcggcga cttccgggtc gagacagtga gcggggacct gagcgtgggc
    10561 ctggtcggcg acgcgacgtt cgagatccgt ggactctcca ccgacgtcag cagcgagctg
    10621 gatcatcggc tcgaaggaca ggtcgatcgg cggcgactga tcgtcggcaa cggcggtgcg
    10681 cggctggtct tcaactcgat gtccggcgac gtggacgtgc gacggccgcg acgactcagc
    10741 gccgaaccaa ggcggacgga gccggttccg gagccgacga cggaagagaa gcaatccgag
    10801 ctcgacgtcc tccgcgcgct cgagcgcggt gagatcgacg tggacgaggc gacccgtcgc
    10861 ctcgccgggg gccgctagat gggcgacgag ctggcgaccg tcctgcgcct ggtggccgag
    10921 gggaagctga gcgccgacga ggctgctccg atcatcgagg cgctgggacg ccacagccct
    10981 ccacgacctc ctgagccgcc tgcagcggtc ccgccattga ccgcgggcaa ggctcgagga
    11041 cgacgcgtcc ggatccaggt caccgagcgc gggcgacgcg tggtcgacgt gcgcgttccg
    11101 ctcgcattcg ccgcgatggc cgcgcgcatg gtgcccggca tcccggacag ctacgccgcg
    11161 ctcatcgagc gggccgtgga ggacgacatg gtcggaccca tcgtcgacct tgccgaggac
    11221 gaggacggcg acggcgtcct gatcaccgtc gaatgacggc caacctgagg cgaacggccg
    11281 atgacatgcg cggccgggtg atgagcatgg tgctgctcgg ctcaattgga ctggcgccgc
    11341 tcagctacgg catcgcgggc gcgatcgtcg atctgggagc ggtcacgatc atgttcgcca
    11401 tcgc
//
LOCUS       DATCQR010000186         3221 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_41634, whole genome shotgun sequence.
ACCESSION   DATCQR010000186 DATCQR010000000
VERSION     DATCQR010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41634"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..842)
                     /gene="truB"
                     /locus_tag="VIN32_06290"
     CDS             complement(<1..842)
                     /gene="truB"
                     /locus_tag="VIN32_06290"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEY8922223.1"
                     /translation="MSWLAAMLGVVNLDKPVGPTSHDMVDLVRRLTAMRRIGHAGTLD
                     PLASGVLPILIGAATRFSDDLTGGPKRYEAVIRLGIRSVTDDAQGPLEPVGPPPDLAT
                     VTAALASFVGTFEQRPPAFSARKTGGVIAHRAARAGNPVEAPPRTVTVESIDMLGSEQ
                     GDGWLDLRCDIRCGAGTYIRSIARDLGNRLGCGGHLHALRRTEAAGLLIADAIAPVLL
                     EELAAAGRLTTAILPVSSLLHLPEVRLEAEAAGRFVHGSAVQVPEATGTGRHAVFGDG
                     ELLG"
     gene            complement(808..1785)
                     /locus_tag="VIN32_06295"
     CDS             complement(808..1785)
                     /locus_tag="VIN32_06295"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEY8922224.1"
                     /translation="MLDVAEVLRGAQRITAFCHENPDGDTLGAGIAIAIAAERLGKQA
                     EVVCVDPPPPFLAFLPGVERVRRAPGLEPDVAVVVDAGELSRIGSVVTEQAEWFARAR
                     IVNIDHHVSNPGYGEAVWIDPEAAATCELVTLLLAELGVPLDAQLATALMAGIVQDTH
                     TFAHPNATARTLRVAAELVEAGAPLAAINRAVYADKPFSTLALWGLMLAGIGQRAGGR
                     IVHASMTAAMLASTAELPTASEGFVDLLASTKLADVTILFKEVGPDETRVSVRTSGRA
                     DAVVITSAFGGGGHARAAGCTVAAPLEAAREPVLAEAERELARGDARGR"
     gene            complement(1785..2138)
                     /gene="rbfA"
                     /locus_tag="VIN32_06300"
     CDS             complement(1785..2138)
                     /gene="rbfA"
                     /locus_tag="VIN32_06300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEY8922225.1"
                     /translation="MSQRTDRLDSQIRQELMGLLQREMKDPRLGFATITRVETARDLG
                     HARVWVSVLGSDAERERTMNALRVATPWLRRRLGELLSLRHVPELSVREDESIASGDR
                     ILQILQELDDEAGEP"
     gene            complement(2149..>3221)
                     /locus_tag="VIN32_06305"
     CDS             complement(2149..>3221)
                     /locus_tag="VIN32_06305"
                     /inference="COORDINATES: protein motif:HMM:NF023413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEY8922226.1"
                     /translation="IAVPVSAKQKIGLDDLMEMILLVADLQDLKANPKRPAIGTIIES
                     KVAKGRGNVATVLVQTGTLKIGDVVTVGRTFGRVRALQNAAGKRVKEAGPATAVEIIG
                     VTDLPEAGDILRVVADEKTARDMAEAVEQAATSGDGKTAFTLEDISAQIQASEVKELR
                     VVLKTDVQGSLGAIRHALERLNMAEVRINIVSEGAGDINESDVQLASASEAVVIGFNT
                     RLDPGAQRAVDALGVDVRLYDVIYKLTDDLGAALSGMLTPKQVEQIEGHAEVRMVIKA
                     GKAGNVAGSYVVDGRIVRGGQVRLFRGGQVVAEGRVESLKRFKDDVREVATGFECGIG
                     LGSIEIAEGDLIECYQMVAEQA"
BASE COUNT          460 a         1181 c         1081 g          499 t
ORIGIN      
        1 atgccgagga gctcaccgtc gccaaatacc gcgtggcgac cggtgccggt cgcctccggc
       61 acctggaccg ccgatccgtg cacgaagcgc cctgccgcct cggcttcaag gcgcacctcg
      121 ggcaggtgca ggagcgaact cactggcagg atcgcagtcg tcagccgccc ggccgccgcc
      181 agttcctcga ggagcacggg cgcgatcgcg tcggcgatca gcaggccggc cgcctcggtc
      241 cgccgcagcg cgtgcaggtg tcctccgcat ccgagccgat tgccgaggtc gcgggcgatc
      301 gaccggatat aggtgcccgc accacagcga atgtcgcacc tcaggtcgag ccacccgtcg
      361 ccctgctccg accccagcat gtcgatcgac tcaacggtca cggtgcgcgg cggcgcctcc
      421 acggggttcc ctgcgcgagc cgctcggtga gcgatcacgc cacctgtctt gcgcgccgag
      481 aacgccggcg gacgctgctc gaaggttccg acgaagctcg ccagcgcggc tgtcacagtg
      541 gccaggtccg gtggcggacc gacgggctcg agcggcccct gtgcgtcatc ggtgacggac
      601 ctgatcccca ggcgaatgac cgcctcatag cgcttcgggc ccccggtcag gtcgtcgctg
      661 aagcgggtcg ccgcaccgat caggatcggc agcacgcccg acgccagcgg gtcgagggtg
      721 ccggcatgcc cgatgcggcg catcgctgtc agccgacgca ccaggtcgac catgtcgtgc
      781 gatgtcggcc cgacgggctt gtcgaggtta acgaccccga gcatcgccgc gagccagctc
      841 acgctcggcc tccgccagga ccggctcgcg ggcggcctca agcggcgccg ccacggtgca
      901 gcccgcggcc cgtgcatgac ctcccccgcc gaaggcggac gtgatgacca ccgcgtccgc
      961 acggccgctg gtgcgaacgc tgacccgcgt ctcgtccggt ccgacctcct tgaagaggat
     1021 cgtcacgtcg gccagcttgg tggatgcgag caggtcgacg aatccctctg atgcggtggg
     1081 caactcagcg gtgctggcca gcatcgccgc cgtcattgac gcgtggacga tgcgtccgcc
     1141 ggcgcgctgc ccgatgcccg ccagcatcag gccccacagg gcaagcgtgc tgaacggctt
     1201 gtcggcgtag acggcccggt tgattgccgc cagcggcgcc ccggcttcga ccagctccgc
     1261 ggcgacgcgc agggtccggg cggttgcatt cggatgggcg aaggtgtgcg tgtcctgcac
     1321 gatgcccgcc atcagcgcgg tcgcgagctg cgcgtcaagc gggacgccga gctcggccag
     1381 gaggagggta accagctcac aggtggcagc cgcctccgga tcgatccaga ccgcctcgcc
     1441 atagccgggg ttgctcacgt gatggtcgat gttcacgatc cgggcccgcg cgaaccactc
     1501 ggcctgctcg gtgaccaccg agccgatccg gctcagctcg ccggcatcga ccaccaccgc
     1561 cacgtccggc tcaagtccgg gggcgcggcg aacgcgctcg actcctggaa ggaaggcgag
     1621 gaaggggggc ggcgggtcga cgcacaccac ctcggcctgc ttgccgagcc gctccgcggc
     1681 gatggcgatc gcgatcccgg cacccagggt gtctccatcg gggttctcat ggcagaaggc
     1741 ggtgatgcgc tgcgcgcccc gcagcacctc ggcgacgtcg agcactatgg ctcccccgcc
     1801 tcgtcgtcga gctcctggag gatctgcagg atgcggtcgc ccgaggcgat cgactcgtcc
     1861 tcgcggacgc tgagctccgg gacatgccgc agggagagca gctccccgag ccggcggcgc
     1921 agccatggcg tcgccactcg cagcgcgttc atcgtgcgct cgcgctccgc atcgctgccc
     1981 aggacgctca cccagacccg agcatggccg aggtcgcgtg ccgtctcgac gcgggtgatc
     2041 gtggcgaagc caaggcgcgg atccttcatc tcacgctgca gcaggcccat cagttcctgg
     2101 cggatctggc tgtcgagtcg atcggtccgc tggctcatcc gcagcgggtc acgcctgctc
     2161 ggccaccatc tggtagcact cgatcaggtc gccctcagcg atctcgatgc tgccgaggcc
     2221 gatcccgcac tcgaagccgg tcgccacctc gcggacgtca tccttgaagc gcttgagcga
     2281 ctcgacccgg ccctccgcca cgacctggcc gccgcggaag agacggacct gccccccgcg
     2341 cacgatccgc ccgtcgacga cgtacgagcc ggcgacgttg ccggccttgc cggccttgat
     2401 caccatccgc acctcggcat ggccctcgat ctgctcgacc tgcttggggg tcagcatgcc
     2461 gctgagtgcc gcgcccaggt catcggtcag cttgtagatg acgtcataca ggcggacgtc
     2521 cacgccgagg gcgtcgacgg cgcgctgcgc gcccgggtcg agccgggtgt tgaagccgat
     2581 gacgaccgcc tccgacgcgc tcgccagctg gacgtccgac tcgttgatgt cgccggcccc
     2641 ttcgctgacg atgttgatcc ggacctccgc catgttgagc cgctcgagtg cgtgccggat
     2701 cgccccgagc gatccctgca cgtcggtctt gagcacgacc cgcagctcct tgacttcgga
     2761 ggcctggatc tgggcgctga tgtcctcgag cgtgaaggcc gtcttgccgt cgccggatgt
     2821 cgccgcctgc tcgaccgcct cggccatgtc gcgggcggtc ttctcgtcgg cgacgacgcg
     2881 caggatgtca ccggcctccg gcaggtcggt gacgccgatg atctccaccg ctgtcgcggg
     2941 ccccgcctcc ttgacgcgct tgccggcggc gttctggaga gcgcgcaccc gtccgaaggt
     3001 cctcccgacc gtgaccacgt cgccgatctt cagcgtcccg gtctgcacca gcacggttgc
     3061 cacgttgccg cgacccttgg caaccttcga ctcgatgatg gtgccgatgg cggggcgctt
     3121 cgggttcgcc ttgaggtcct gcaggtcggc gaccagcagg atcatctcca tcaggtcgtc
     3181 gagcccgatc ttctgcttgg ccgagaccgg caccgcgatg a
//
LOCUS       DATCQR010000187         2606 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_165332, whole genome shotgun sequence.
ACCESSION   DATCQR010000187 DATCQR010000000
VERSION     DATCQR010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2606
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165332"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..936)
                     /gene="gap"
                     /locus_tag="VIN32_06310"
     CDS             complement(<1..936)
                     /gene="gap"
                     /locus_tag="VIN32_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEY8922227.1"
                     /translation="MTRIGINGFGRIGRQTLKAILERHPDTLEVVAVNDLAPTATNAH
                     LFKYDSTYGRYSGEVSHTDSDIVIDGYPIRAFSERDPAALPWKDLGVEIVVESTGIFT
                     DATKAHAHLDAGARKVIISAPAKNEDVTLVLGVNEGVYDPEKHHIVSNASCTTNGLAL
                     PAKVVWDSFGIERGLMTTVHSYTNDQNVLDVFHSDLRRARSAGQNIIPTSTGAAKALA
                     LVIPELKGRFDGFSLRVPTATVSVIDFVAITSRPVTAETANAALKTAADGPMQGLLGF
                     TAEPLVSMDFKGDERSSIVDSLSTMVVGDNLLKVIA"
     gene            complement(933..1934)
                     /locus_tag="VIN32_06315"
     CDS             complement(933..1934)
                     /locus_tag="VIN32_06315"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEY8922228.1"
                     /translation="MDEVADRSILAIDLGGTQIRAAHVSPDLTVSCRRALETHDEEGV
                     EAVIDRICAAARAVREDAQRAGLPDPVGIGISSPGPLDPWRGVILATPNLAGWRNIPL
                     AARVEDAVGLPTYLERDTNAAVMGEWRYGAAKGADDVIYVTVSTGVGGGLVLDGRPLI
                     GKDGTAGEIGHMTVDIDGPLCGDGQPGHAEAIGSGRAIAREGRSLLKNGQAPGLAALA
                     ADAEVDAELVARAADAGDAACQRVLDRAWVAIGAMCASLVNALNPEVIVLGGSIGCNR
                     PELPSVIRAEIDRRAFSAPARRVRLEMTQFAGDVSLIGLLPIVNERMADPSYAKEHA"
     gene            complement(2084..2227)
                     /locus_tag="VIN32_06320"
     CDS             complement(2084..2227)
                     /locus_tag="VIN32_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5679 domain-containing protein"
                     /protein_id="HEY8922229.1"
                     /translation="MATNAYCVKCKAKRDMRDESKITMKNGKPATQGTCPVCGTKMFK
                     IGG"
     gene            complement(2271..>2606)
                     /locus_tag="VIN32_06325"
     CDS             complement(2271..>2606)
                     /locus_tag="VIN32_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8922230.1"
                     /translation="PQGRAMRTLPVRYPDLAEELRRQRVANLEMESSALFVLAGLAGL
                     RAGTVCAAYAQRVDGTFLEGAAKEAAEARCIDVGLAGIHLLWVIDNGHEGDPHARWSH
                     LIGGQGGER"
BASE COUNT          388 a          905 c          873 g          440 t
ORIGIN      
        1 ggcgatgacc ttcagcaggt tgtcgcccac caccatggtc gagaggctgt cgacgatgct
       61 ggatcgctcg tcacccttga agtccatgct caccagcggc tcggcggtga agccgagcag
      121 cccctgcatc ggtccgtccg ccgcagtctt gagtgccgcg ttggcggtct cggcggtgac
      181 ggggcgcgag gtgatcgcca cgaagtcgat gacgctgacc gtggcggtcg gcacgcgcag
      241 cgagaagccg tcgaagcggc ctttcagctc ggggatcacg agagcgagcg ccttggccgc
      301 gccggtcgag gtggggatga tgttctgccc cgccgagcgc gcacgacgca ggtccgagtg
      361 aaacacgtcg agcacgttct ggtcgttggt gtaggagtgg accgtcgtca tcaggccccg
      421 ctcgatcccg aacgagtccc acacgacctt ggccggcagc gccagcccgt tggtggtgca
      481 cgacgcgttg ctcacgatgt ggtgcttctc cgggtcgtag acgccctcgt tcacgccgag
      541 caccagcgtc acgtcctcgt tcttggccgg ggcgctgatg atcaccttgc gagctcccgc
      601 gtcgaggtgg gcgtgcgcct tggtggcatc cgtgaagatg ccggtcgact ccaccacgat
      661 ctcgacgcca aggtccttcc acggcagcgc cgccggatcc ctctccgaga aggcacggat
      721 gggatagccg tcgatgacga tgtcgctatc ggtgtgcgac acctcgcccg aatagcgccc
      781 gtaggtcgag tcgtacttga acaggtgcgc gttggtggcg gtcggcgcca ggtcgttgac
      841 ggccaccacc tcgagggtgt ccgggtggcg ctccaggatg gccttgaggg tctggcggcc
      901 gatccgcccg aagccattga tgccgatgcg ggtcacgcat gctccttcgc ataggacggg
      961 tctgccattc gctcattcac gatcggcagc aggccgatca acgagacgtc gccggcgaac
     1021 tgcgtcatct cgagccgcac gcggcgcgcc ggagccgaaa aggcacggcg gtcgatctcg
     1081 gcccggatca cgctcggcag ctctggccga ttgcagccga tgctgccgcc gagcacgatc
     1141 acctcgggat tcagggcgtt caccaggctc gcacacatgg cgccgatcgc aacccaggcg
     1201 cggtcgagaa cgcgctgaca ggcggcgtcg cccgcgtcgg cggctcgcgc caccagctcg
     1261 gcgtcgacct cagcgtcggc cgcgagagcc gccaggccgg gcgcctggcc gttcttcagc
     1321 agcgagcgac cttcccgtgc gatggcccgg ccggagccga tcgcctcggc gtgaccgggc
     1381 tggccgtcac cgcacagcgg accgtcgatg tcgaccgtca tgtgcccgat ctcgccggcg
     1441 gtcccgtcct tgccgatcag cggccggccg tcgagcacca ggccgccacc aacgccagtt
     1501 gagaccgtca cgtagatcac gtcatcggca cccttggccg cgccgtagcg ccactcgccc
     1561 atgacggctg cgttcgtgtc gcgctcgagg taggtgggca acccgaccgc atcctccacg
     1621 cgcgcggcga gcgggatgtt gcgccagcca gcgaggttgg gggtcgccag gatcactccc
     1681 cgccagggat ccaacggacc gggcgacgag attccgatcc cgaccgggtc ggggaggccg
     1741 gcgcgctgcg cgtcctcccg cacggcccga gcagcggcgc agatacgatc gatcactgct
     1801 tccacgccct cctcgtcatg ggtctccagg gcacggcggc acgagacggt caggtcggga
     1861 ctgacgtgcg cagcgcggat ctgggtcccg ccgaggtcga tcgccaggat cgaccggtcg
     1921 gctacctcat ccacaggccg ctcattgtag cggccgatac ggcatcggcc gctcggcggc
     1981 ggccatcggg actcccgacg gcgcgcgacc gaacggccga cgcgcttggt attgagcgtc
     2041 gcgggctgaa cccgcgagct ggatcagatc tgcaggccct ggttcagccg ccgatcttga
     2101 acatcttggt gccgcacacc gggcaggtgc cctgggtggc cggcttgccg ttcttcatgg
     2161 tgatcttgct ttcgtcgcgc atgtcgcgct ttgccttgca cttgacgcag taggcattgg
     2221 tcgccatcgg tcagttgcct ccttgtgtgc cggcctggtg ccggcgttcc ctaacgctcc
     2281 ccgccttgcc cgccgatcaa gtgtgaccag cgtgcgtggg gatcgccttc gtgtccgttg
     2341 tcgatcaccc agagcaggtg gatgccggcc agtccgacat cgatacagcg tgcctctgcg
     2401 gcctccttcg ccgcgccttc cagaaacgtc ccatcgacgc gctgtgcgta ggccgcacac
     2461 accgtccccg cgcgcaaccc cgcgagtcct gccaacacga agagtgccga cgactccatt
     2521 tcgaggttgg cgacgcgctg gcgtcgcagc tcctccgcga gatcaggata gcgaaccggg
     2581 agcgtgcgca tcgcccttcc ctgcgg
//
LOCUS       DATCQR010000188         6780 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_226582, whole genome shotgun sequence.
ACCESSION   DATCQR010000188 DATCQR010000000
VERSION     DATCQR010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6780
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226582"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..456)
                     /locus_tag="VIN32_06330"
     CDS             complement(<1..456)
                     /locus_tag="VIN32_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018568680.1"
                     /note="catalyzes the thiolytic cleavage of
                     beta-ketoadipyl-CoA to succinate and acetyl-CoA; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipyl-CoA thiolase"
                     /protein_id="HEY8922231.1"
                     /translation="MRQHDPARTPVVVDGLRTPFGRYGGALREVRPDDMAAHVIRALL
                     ERTGVDPGAVEDVILGAANQAGEDNRNVGRMAALLAGMPIEVAGQTVNRLCGSGMQAV
                     IDAAHAITAGDGDLFVAGGVESMTRAPFVMAKASAAFPRGEQTLYDTTLG"
     gene            495..1931
                     /locus_tag="VIN32_06335"
     CDS             495..1931
                     /locus_tag="VIN32_06335"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY8922232.1"
                     /translation="MTAQAPPELPIPGQRRAWWLREALATEGPSLLAPSLFGEVEADV
                     AIVGGGYTGMWTAYFLGEVAPETRIVLLEADICGGGPSGRNGGFLHGWWEYLPYLATR
                     YGPAEALRIAEASDEVVTGIGEWCRRHAVDAWFTPHGYLRVNAFPSLPNDWGDTIAEM
                     DRLGAPGKLVHMDRLAVQGMCASPAFGEGLFMPSAATVQPARLARGLRRVLLERGVQI
                     HEGTGVERIESSAGGVHLTTARGSVRAERAVLAVNAWAAGWPGFRTRVLAWGSYMVLT
                     EPIPDRLTELGWTGGEAIADSRFTISYFRTTRDGRIAFGAGVGGAGFDGRIGPVFTHD
                     RQAIERVVANFRHLFPMLSDVRFDDAWGGPIDITADRFPEIGSQDGGRVLYAHGFAGN
                     GVGPARLAGRILAARIAGAGDPVGSLPLFGRRQRFLPPEPFRFVGARAIREALIRQDD
                     ALDAGRRPGLLTRLVALAPSLLGYRFKH"
     gene            complement(1945..2166)
                     /locus_tag="VIN32_06340"
     CDS             complement(1945..2166)
                     /locus_tag="VIN32_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008872625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 domain-containing protein"
                     /protein_id="HEY8922233.1"
                     /translation="MCRSIKTLRTLDEPAPDEEVRAAALQYVRKVSGYRKPSPANEHA
                     FNHAVEEIAIASSRLLQAVTATHPARRAS"
     gene            complement(2177..2824)
                     /locus_tag="VIN32_06345"
     CDS             complement(2177..2824)
                     /locus_tag="VIN32_06345"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEY8922234.1"
                     /translation="MSELLHRYKAVIFDMDGLLLDTETLWQEAEEALFAAHGGVFSRE
                     DKLRVIGTSFDFTARYFAERLGEPPEHGAELVEEMIETMYAALQREVAGRPGAVELVA
                     RLRGRMPLGLASNSPRRLVDAALATAGLTDVFAAIVTSEDVAHSKPAPDIYLEACRRL
                     GASPTETLALEDSASGVAAAKAAGLTCIAVPQYAETDVTAADQVIDSLEALLAEA"
     gene            complement(2821..4038)
                     /locus_tag="VIN32_06350"
     CDS             complement(2821..4038)
                     /locus_tag="VIN32_06350"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEY8922235.1"
                     /translation="MGADAAPRALERIGVLGAGTMGAGIAQLAAEAGIEVIVHDPIER
                     AYDHALERTAGFLARKVEKGQLSAASREAALARIAPAATVEALAAADLVIEAIPEDLH
                     LKRDAFRRLDAAAAATTILASNTSSLSIGAIAAATGRPERVVGMHFFNPVPLMALVEV
                     IAGASTGQEVAEATMAAARRLGKTPVLAADTPGFIVNRVARPFYLEAMRIAGVGSASI
                     EEIDVAARGVGFRMGPFELVDTIGADVNLAVSQSVYEAFGRDPRYRPHEMQHAVVDAG
                     RLGRKTGGGFYDYSPDGARGPAWSGLAPVPTGERLTESQIAERLLATIVNEAASAVAD
                     SVSPPESIDTAMRLGANYPSGPLEWGERIGLIQVVRALDTLGARDPNGSYRVVPLLRS
                     LAESDGSFFAPRP"
     gene            complement(4028..4813)
                     /locus_tag="VIN32_06355"
     CDS             complement(4028..4813)
                     /locus_tag="VIN32_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEY8922236.1"
                     /translation="MPEASYETILVDVADGVATITLNRPDSLNALNGAMRRELLAAFK
                     GAGRDEAIRAVVLTGAGRGFCSGADLRGGDEERAFRRVLTDEYNPLILAIRELPKPVI
                     AAVNGVAAGAGVSIALACDLVYASEEARFIQAFVRIGLVPDSGSTRTLVRALGRHRAA
                     ALIFSGEPLGATEAHAIGLVNEVVAADQLTVTVQAAAAKLAAAPTRAIGLAKRAINHA
                     EDALMAESLAMEASLQELAGRTEDHAEGAAAFGEKREPRFVGR"
     gene            complement(4837..6090)
                     /locus_tag="VIN32_06360"
     CDS             complement(4837..6090)
                     /locus_tag="VIN32_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020387209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8922237.1"
                     /translation="MRNLPAASLWRDQGFRTFWSAQAVSELGDRITELALPLIAVTLL
                     NASPTQVGLLTAAVWLPNLASLFIGTWVDQRRDKRPLMIAADLSRMVVLLSLPAAYWL
                     DLLTLGQLYAIAILAGTAHVVFNTAYASFFVRLVTREQFLEANSKLSATRSISFMAGP
                     AAGGLLIQWLTAPVAIVVDAASFLFSAIQVSRLKTPVLEPEPATESLLRRARAGMGYL
                     LRHPYLRSSLGCATTLNFFNLIGSALLILYASRNLALSAGTIGLAFGIGASGGLLGAL
                     AAAPLTRAIGAGRLIAIGAVVFPAAIGIAALASGPVLLRATALAAAEFVGAFAVMCFD
                     IPLNAIQASVTHEGMRSRVAGAFSSINYGVRPLGAVVGGLLGTWLGVRETLLISSAGG
                     LLAVLWLIRSPIIHVRSIEGLQPPD"
     gene            6145..6522
                     /locus_tag="VIN32_06365"
     CDS             6145..6522
                     /locus_tag="VIN32_06365"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY8922238.1"
                     /translation="MDRSDIERWLGAYLRAWRTDDPQDIAALFTEDATYAPRPFGKAW
                     EGRDAIVANWIELGDSKSSWQFESEIVAVDGDTGVVRGLTTYPAHDNEPEEVYSNIWV
                     VKLAPDGRAIWFAEWWVELPKPG"
     gene            complement(6527..>6780)
                     /locus_tag="VIN32_06370"
     CDS             complement(6527..>6780)
                     /locus_tag="VIN32_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEY8922239.1"
                     /translation="LVARVVPNELLVEDALAVAARIATKSPLALRMAKEAVNAAYEMS
                     LTDALAHERRLFYLLFASDDQKEGMAAFLEKRDPDFKGR"
BASE COUNT         1057 a         2333 c         2373 g         1017 t
ORIGIN      
        1 gccgagggtc gtgtcgtata gcgtctgctc gccacgcggg aatgccgcgg acgccttggc
       61 catcacgaac ggggcacgag tcatcgactc gacgcccccc gccacgaaca ggtccccgtc
      121 gccggcggtg atcgcgtgag cagcgtcgat gaccgcctgc attcccgatc cgcacagacg
      181 gttgacggtc tggcctgcca cctcgatcgg catcccggca agcagtgccg ccatgcggcc
      241 gacgttgcgg ttgtcctcgc cggcctggtt ggcggccccc aggatcacgt cctcgacggc
      301 accggggtct acacccgtgc gttcgagcag tgcgcggatg acatgcgcgg ccatgtcatc
      361 aggcctcacc tcgcgcaggg cgccgccata gcggccgaag ggcgtccgca gcccgtcgac
      421 gaccaccgga gtccgagctg gatcgtgctg gcgcatcagg cgtggatgct agcaccgggt
      481 taggatcgcg cgacgtgacc gcccaagcgc cacctgagct gccgatcccc ggccaacgcc
      541 gtgcatggtg gctgcgcgag gccctggcca ccgaagggcc gagcctgctc gccccctcgc
      601 ttttcggcga ggtggaggcc gacgtcgcga tcgtgggtgg cggctacacc ggcatgtgga
      661 ccgcctactt cctgggtgag gtggcgccgg agacccggat cgtgctgctc gaggcggaca
      721 tctgcggcgg cggcccgagc ggtcggaacg gcgggttcct gcacggctgg tgggagtacc
      781 tcccctacct ggccacgcgc tacggcccgg ccgaggccct gcgcatcgcc gaggcatccg
      841 acgaggtggt gaccggcatc ggtgaatggt gccggcgtca cgcggtcgat gcctggttca
      901 cgccgcacgg ctacctgcgc gtcaacgcct tcccgagcct gcccaatgac tggggcgaca
      961 cgatcgccga gatggatcgg ctcggcgctc cggggaagct ggtgcacatg gaccgactgg
     1021 ccgtgcaggg gatgtgcgcc tcgcccgcgt tcggcgaggg tctcttcatg ccgagcgccg
     1081 ccaccgtcca accggcccgc ctggcacggg ggctgcgccg tgtcctgctc gagcgcggag
     1141 tccagatcca cgagggaacg ggcgtcgaga ggatcgaatc cagcgccggc ggcgtgcacc
     1201 tgacgacggc tcgcgggagc gtccgcgccg agcgggcggt cctggccgtg aacgcctggg
     1261 ccgctggctg gccgggcttc cggacccggg tcctggcctg gggcagctac atggtcctga
     1321 ccgagccgat ccccgaccgg ctgaccgagc tcggctggac cggcggcgaa gcgatcgccg
     1381 actcgcgctt cacgatcagc tacttccgaa ccacgcgtga tgggcgcatc gccttcggcg
     1441 caggagtcgg cggggccggc ttcgacgggc gcatcggccc ggtcttcacg cacgatcgcc
     1501 aggcgatcga gcgcgttgtc gcgaacttcc gccacctctt cccgatgctc agcgacgttc
     1561 ggttcgacga cgcctggggt ggaccgatcg acatcaccgc ggaccggttc cccgagattg
     1621 gctcgcagga tggcggtcgc gtcctgtatg cccatggctt tgccggaaac ggggtgggac
     1681 cggcccgcct ggcgggacgc atcctcgccg cgcgcatcgc cggtgctggc gatccggtcg
     1741 gctccctgcc gctcttcggc cgacgccagc ggttcctgcc tcctgagcca ttccggttcg
     1801 tcggggcgcg agccatccga gaggcgctca ttcgccagga cgacgcgctc gatgccgggc
     1861 gtcgaccggg gctccttacc cggcttgtgg cgctcgcgcc gagcctgctg ggctatcggt
     1921 tcaagcattg acgccgatga gggtctagct cgcgcgacgg gcaggatgcg tcgcggtcac
     1981 ggcctgcagc agccggctcg acgctatggc gatctcctcg acggcatggt tgaaggcatg
     2041 ctcattggca ggcgagggct tgcgatagcc actcaccttg cgaacgtact gcagggcggc
     2101 ggcgcggacc tcttcatcgg gggccggctc gtcgagggtt cgcagggtct tgatgcttcg
     2161 gcacatgctc gcaagtctac gcctcggcca ggagcgcctc gagcgagtcg atgacctgat
     2221 cggctgccgt gacatcggtc tcggcgtact gcggcacggc gatgcaggtc agcccggcgg
     2281 ccttggctgc ggccacgcct gacgccgagt cctcgagtgc aagggtctcc gtcggactgg
     2341 ccccgagccg gcggcacgcc tcgagataga tgtcgggggc cggcttggag tgggcaacgt
     2401 cctcggacgt cacgatcgcg gcgaagacat cggtcaggcc ggcggtggcc agagcagcgt
     2461 cgaccaggcg gcggggcgag ttggaggcga ggccgagcgg catccgccca cgcagccggg
     2521 ccaccagctc gacggctccc gggcgtcccg cgacctcacg ctgaagggcg gcgtacatcg
     2581 tctcgatcat ctcttccacg agctcggcgc catgctcggg aggctcaccg agccgctcgg
     2641 cgaagtagcg ggcggtgaag tcgaacgagg tcccgatcac ccgcagcttg tcctcccggc
     2701 tgaagactcc gccgtgcgcc gcgaagagtg cctcctcggc ctcctgccag agcgtctcgg
     2761 tatcgagcag caggccatcc atgtcgaaga tgaccgcctt gtaccgatgc agcagctcgc
     2821 tcacgggcgt ggcgcgaaga aggatccatc gctctcggcg agggagcgca ggagcggcac
     2881 gacccggtag ctcccattcg gatcccgggc gcccagcgtg tccagggcgc gcaccacctg
     2941 gatcaagccg attcgctcgc cccattccag cggtccggag gggtagttgg cacccagccg
     3001 catggcagta tcgatcgatt ccggaggcga aacgctgtcg gccaccgccg acgccgcctc
     3061 attcacgatc gtggccagca gccgttccgc gatctgcgac tcggtcaggc gctccccggt
     3121 tggcacgggt gccagtccgg accaggcagg gccgcgcgcg ccatcgggtg agtagtcgta
     3181 gaagccgcct ccggtcttac gccccaggcg cccggcatcg acgacggcat gctgcatctc
     3241 atgcgggcgg tagcgcggat cgcgaccgaa cgcctcgtac acgctctggc tgaccgccag
     3301 gttgacgtcg gcgccgatgg tgtccaccag ctcgaacggt cccatccgga agccgacccc
     3361 gcgcgccgcc acgtcgatct cctcaatgga ggccgagcca acgccggcga tccgcatcgc
     3421 ctcgaggtag aagggacgcg ccacgcgatt gacgatgaag cccggcgtat ccgccgccag
     3481 cacgggcgtc ttgcccagcc tgcgggcagc cgccatggtc gcctccgcaa cctcctggcc
     3541 ggtgctcgcg ccggcgatca cctcaaccag cgccatcagc gggacggggt tgaagaagtg
     3601 catgccaacc acccgctccg gccggcccgt ggcggcggcg atggcgccga tcgagaggct
     3661 gctggtattg ctcgccagga tggtggtcgc ggcagccgcc gcatccaggc gacggaaggc
     3721 gtcgcgcttg aggtgcaggt cctcggggat cgcctcgatc acgaggtccg cggctgccag
     3781 cgcctccacc gtggcggcgg gcgcgatgcg ggccagcgcg gcctcgcggg acgcggcgct
     3841 gagctgcccc ttctcgacct tgcgggcgag gaagccggcc gtgcgctcga gcgcgtggtc
     3901 gtacgctctc tcgatcgggt cgtggacgat gacctcgatc cccgcctccg cagccagctg
     3961 ggcgatgccc gcccccatcg tgccggcacc caggaccccg attcgctcga gggcacgagg
     4021 cgcggcgtca gcgcccaacg aagcgcggct cccgcttctc gccgaacgcg gccgccccct
     4081 cggcgtggtc ctcggtgcga cccgccagct cctggagcga tgcctccatg gccaagctct
     4141 cggccatcaa cgcgtcttcg gcatggttga tcgcccgctt tgccaggccg atcgcacgcg
     4201 ttggcgccgc ggccagcttc gccgccgcgg cctgcacggt aacggtcagc tggtccgccg
     4261 ccacgacctc gttgaccagg ccgatggcgt gggcttcggt ggctcccaac ggttccccgc
     4321 tgaagatcag tgcagccgcc cgatgccggc ccagcgcgcg gaccagggtt cgggtcgagc
     4381 ccgaatcggg caccaggccg atccgcacga aggcctggat gaaccgcgcc tcctcgctgg
     4441 cgtagacgag gtcacaggcc agggcgatgg agaccccggc tccggccgcg acgccgttga
     4501 ccgcggcgat cacgggcttc gggagctccc ggatggccag gatcagcggg ttgtactcat
     4561 cggtcagaac gcgccgaaag gcacgctcct cgtcaccgcc acgaagatcg gctccactgc
     4621 agaatccacg gcccgcgccg gtgagaacca ccgctcggat cgcctcgtcg cgcccggcgc
     4681 ccttgaatgc ggccagcagc tcgcgccgca tggcgccgtt gagtgcgttg agcgagtcgg
     4741 ggcggttcag ggtgatggtg gccacgccat cggccacgtc gaccaggatc gtttcgtagc
     4801 tggcctctgg catgtggcgg attctatcgc caccgatcag tccggcggct gcagcccctc
     4861 gattgagcgg acgtggatga taggcgagcg gatcagccac agaaccgcga gcaggccgcc
     4921 cgccgaggag atcagcagcg tctcgcggac cccgagccag gtcccgagca gcccgccgac
     4981 gacggctccc aacggccgca cgccgtagtt gatgctgctg aacgcacctg ccacgcggct
     5041 tcgcattcct tcgtgggtga ccgatgcctg gatcgcgttc agcgggatgt cgaagcacat
     5101 caccgcgaag gccccgacga attcggcggc cgcgagtgcg gtcgcgcgca agaggacagg
     5161 gcccgatgcg agggcggcaa tcccgatagc ggccgggaag accaccgcac cgatggcgat
     5221 cagccggccg gcaccgatcg cgcgggtcag cggggcggcg gcgagcgcgc cgagcaggcc
     5281 accggatgcc ccgatgccga acgccagccc gatcgtcccc gccgacagcg cgaggttcct
     5341 gctcgcatac aggatcagca gtgccgatcc gatcaggttg aagaagttca gcgtcgtcgc
     5401 gcagcccagg ctggaccgca ggtacggatg ccgaagcagg tatcccatcc ccgcccgcgc
     5461 tcgccgaagc aacgactcgg tggccggctc tggctcgagg accggggtct tcagccggct
     5521 gacctggatc gccgagaaca ggaaggaggc ggcgtcgacc acgatcgcga cgggtgcggt
     5581 cagccattgg atcagaaggc cgccggccgc tggaccggcc atgaaggaga tcgacctcgt
     5641 cgccgagagc ttgctgttgg cttcgaggaa ctgctccctg gtgaccagtc gcacgaagaa
     5701 cgatgcgtag gcggtgttga acacgacatg ggcggtccca gccaggatcg caatggcgta
     5761 aagctgtccg agcgtcagca gatccagcca gtaggccgcg gggagggaga gcagcaccac
     5821 catccggctg aggtcggccg cgatcatcaa tggccgcttg tcccggcgct ggtcgaccca
     5881 ggtgccgatg aacagcgagg ccaggttcgg cagccagact gccgcggtga gcaggccgac
     5941 ctgggtgggc gaggcgttca gcagcgtgac cgcgatcagc ggcagggcaa gctcggtgat
     6001 ccgatcgccc aattccgaga ccgcctgcgc ggaccagaac gttcggaacc cttgatcgcg
     6061 ccacaggctg gcggcaggca gattcctcat cgttgaatat tgaccggaga cgctcgacga
     6121 atggcgcgtg aggaggttga tgacatggat cggagcgaca tcgaacgctg gctgggcgcc
     6181 tacctgcgtg cctggaggac cgatgaccca caggacatcg ccgctctctt caccgaggac
     6241 gcgacctatg cgccacggcc cttcggcaag gcatgggagg ggcgggatgc gatcgtggcg
     6301 aactggatcg agctgggaga ttcgaagagt tcgtggcagt tcgagtcgga gatcgtggcg
     6361 gtggatggcg acacgggcgt ggtgcgtggc ctgaccacct accccgccca cgacaacgag
     6421 ccggaagagg tctacagcaa catctgggtg gtcaagctgg cgccggatgg ccgggcgatc
     6481 tggttcgccg agtggtgggt ggagctccca aagccagggt gagcggctag cggcccttga
     6541 agtcggggtc ccgcttctcc aggaatgccg ccatcccctc cttctggtca tcggaggcga
     6601 agagcaggta gaagaggcgg cgctcgtgcg ccagcgcatc ggtcaggctc atctcgtagg
     6661 ccgcgttgac cgcttccttg gccatccgca gcgcgagcgg cgacttggtc gcgatcctgg
     6721 ccgccactgc cagcgcatct tcgacgagca gctcgttcgg aacgactcgg gcgaccaacc
//
LOCUS       DATCQR010000189         3686 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_165371, whole genome shotgun sequence.
ACCESSION   DATCQR010000189 DATCQR010000000
VERSION     DATCQR010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3686
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165371"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..894
                     /locus_tag="VIN32_06375"
     CDS             <1..894
                     /locus_tag="VIN32_06375"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY8922240.1"
                     /translation="IMFYAMLALINEGDEVIYPNPGFPIYESMVRYVGGTAVPAPLRE
                     ANDFRMDVAEVASLVTDRTRMIVTNSPHNPTGSILTQDDVREIARIAVEHDLVVLADE
                     IYGRLQYEGEPLSIATLPGMAERTITLDGFSKTFAMTGWRLGYGIVPTWLMPSFSALV
                     INSVSGTNAFAQAGAVAALTGPQDEADAMRAEFIARRSLIVEGFNAIPGVSCVMPHGA
                     FYAFPNMTSFGRSSNEIADHLLYDAGVCGLSGTAFGSYGEGYLRFSYANSREKITAAL
                     ERVGESLAKLEKTGELVGASA"
     gene            903..3272
                     /locus_tag="VIN32_06380"
     CDS             903..3272
                     /locus_tag="VIN32_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013291347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEY8922241.1"
                     /translation="MTTIQQRTADRLYDEAVPSFERWDAVREIIDEAIDLALNYRQSG
                     HPGGSRSKVHMFLSLLLSGAMRWDIRTPWRPFADRFVLSAGHTIPLVYATLAVLNETL
                     RARAERDPDPAFAFPDDGRWALTWEDLLKLRRRGGLPGHAEMEGKTLFIKFNTGPSGH
                     GQPPSAGEAVALKLAGAGEVKVFTVEGEGGLTPGASHETRNSAWGLGLNNLVYLLDWN
                     DYGIDDPAISSVVPGTPESWFNAYSWRVTGTMEGSEWGPVTRAVLEAARGENPDRVPS
                     VAWFKTRKGRGYGKYDNKSHGTPHLMNSPEFWAVRKEFMARHGVEYAGVDEPAPADAK
                     EQQAQARDNFGVAMGVLRANTELVDAISDRLVEVAGLVPDQVEGFNLGGRGAEIFSDK
                     RLTDAHAYPAEMWKAPGEKAPNRAALATWGSWVNAYAKQEYGRPLFIVCSADLAESTN
                     IAGFAKDFGDMPGWGWYQRDTNPRGALLPQQITEFTNSGVTVGIATVNLADDPMTSFN
                     GFWAACSTYGSFSYLKYGPMRLFSQLAQDCELKVGKVIWVAGHSGPETAEDSRTHFGI
                     YETGVTQLFPEGHVIDLHPWEYNEVPVVLAAALATDVPIIALHLTRPAVDIPDRAVLG
                     MASHFEAARGAYLIRDVAAGTPSAGTVYVQGTVTTASVVKALPQLDERGINVRIVACI
                     SPQLFRRQDEAYRDGICSASDRWSGMAITNRAFKLMTDFVEGPIAKEYSLSSDWDNRW
                     RTGGTVDEVMDEAHLGPGHILEAIERYASEREERHARLREIVASLERLG"
     gene            3281..3487
                     /locus_tag="VIN32_06385"
     CDS             3281..3487
                     /locus_tag="VIN32_06385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8922242.1"
                     /translation="MSETHPHTYEHQRPRRPTHLARRTTPPPERPAPPNLRYLLYVGL
                     ALTAVVVALAVPYVIGYLLQLVTG"
     gene            complement(3484..>3686)
                     /locus_tag="VIN32_06390"
     CDS             complement(3484..>3686)
                     /locus_tag="VIN32_06390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8922243.1"
                     /translation="EWKDLRCPCHGASFNLAGQLANGSEGWSSGSGYRGDEQAYPIDL
                     PPLVRPEIKVSGETVLVWTAKA"
BASE COUNT          601 a         1275 c         1241 g          569 t
ORIGIN      
        1 atcatgttct acgcgatgct cgccctcatc aacgaaggcg acgaggtcat ctacccgaac
       61 ccgggcttcc cgatctacga gagcatggtc cgatacgtcg gcgggaccgc cgtgccggcg
      121 ccgctgcgcg aggccaacga cttccgcatg gacgtcgccg aggtggcatc cctggtgacc
      181 gatcggaccc ggatgatcgt caccaactcg ccgcacaacc cgacggggtc gatcctgacc
      241 caggacgacg tgcgcgagat cgcccggatt gcggtcgagc acgacctggt cgtcctggcc
      301 gatgagatct acggccgcct ccagtacgag ggcgagccgc tctccatcgc cacgctgccc
      361 ggcatggcgg agcgcacgat cacgctcgac ggcttcagca agaccttcgc gatgacggga
      421 tggcggctcg gctacgggat cgtgccgacc tggctcatgc cgtccttctc ggcactcgtc
      481 atcaactcgg tgagcggcac caatgccttc gcccaggccg gggcggtggc ggccctgacg
      541 gggccgcagg acgaggccga tgccatgcgc gccgagttca tcgcccgtcg atcgctgatc
      601 gtcgagggct tcaacgccat cccgggggtg agctgcgtca tgccgcacgg ggccttctac
      661 gccttcccga acatgacgtc cttcggccga tccagcaacg agatcgccga tcacctgctc
      721 tacgacgcgg gcgtctgcgg cctctccggc accgccttcg gcagctacgg cgagggctac
      781 ctgcgcttca gctacgccaa cagccgcgag aagatcaccg ccgcgctgga gcgcgtcggc
      841 gagtcgctgg ccaagctcga gaagaccggc gagctcgtcg gcgcatcggc ctagcaagca
      901 cgatgacaac catccagcag cgcacggctg atcgcctcta cgacgaggcg gtcccctcgt
      961 tcgagcggtg ggacgcggtg cgggagatca tcgacgaggc gatcgacctg gcgctgaact
     1021 accgccagag cgggcacccc ggaggttcgc gctccaaggt ccatatgttc ctgtcgctgc
     1081 tcctgtcggg agcgatgcgc tgggatatcc gcacgccgtg gcgacccttt gcggaccgct
     1141 tcgtgctgtc ggccggtcat acgatcccac tggtctacgc caccctggcg gtcctgaacg
     1201 agacgctgcg cgcccgcgcc gaacgggatc cggatcccgc cttcgccttc cccgatgacg
     1261 gtcgctgggc gctcacctgg gaggacctgc tcaagctgcg ccggcgcggc gggctgcccg
     1321 gccacgccga gatggagggc aagaccctct tcatcaagtt caacaccggg ccgtccggtc
     1381 acggccagcc gccttccgcc ggtgaggcgg tggcgctcaa gctcgccggc gccggggagg
     1441 tcaaggtctt cacggtcgag ggtgagggtg gcctgacccc cggcgccagc cacgagaccc
     1501 gcaactcggc ctgggggctg ggactcaaca acctcgtcta cctgctcgac tggaacgact
     1561 acgggatcga cgacccggcg atctcgagcg tggtgcccgg cacccccgag tcctggttca
     1621 acgcctattc ctggcgcgtg acggggacca tggagggctc ggaatggggt cccgtgacgc
     1681 gcgcggtgct ggaggcggcg cggggcgaga acccggatcg cgttccatcg gtcgcctggt
     1741 tcaagacccg caaggggcgt ggctacggca agtacgacaa caagagccac ggcactccgc
     1801 acctcatgaa ctcgcccgag ttctgggcgg tgcgcaagga gttcatggcc cgccacgggg
     1861 tcgagtacgc cggcgtggac gagccggcac ccgctgacgc gaaggagcag caggcgcagg
     1921 cccgcgacaa cttcggggtg gcgatgggcg tcctgcgagc caacaccgag ctggtggatg
     1981 ccatcagcga ccggctggtc gaagtggcgg ggctcgtgcc ggaccaggtc gagggtttca
     2041 acctgggcgg gcgtggcgcc gagatcttca gcgacaagcg gctcaccgac gcgcacgcct
     2101 atcccgccga gatgtggaag gcgcccggcg agaaggctcc gaaccgggct gcgctggcca
     2161 cctggggctc atgggtcaat gcctacgcga agcaggagta cgggcggccg ctgtttatcg
     2221 tttgctcggc ggacctggcg gagtcgacca acattgccgg cttcgccaag gacttcggcg
     2281 acatgcccgg ctggggctgg taccagcgcg acacgaaccc gcgcggcgcg ctgctgccgc
     2341 agcagatcac cgagttcacg aactccggcg tgacggtcgg gatcgccact gtcaacctgg
     2401 cggatgaccc catgacctcc ttcaacggtt tctgggctgc ctgctcgacc tatggctcgt
     2461 tctcgtacct caagtacgga ccgatgcgcc tcttcagcca gctcgcgcag gactgtgagc
     2521 tgaaggtcgg caaggtgatc tgggtggcgg ggcactccgg gcccgagacg gccgaggact
     2581 cgcgcacgca cttcgggatc tacgagacgg gcgtcacgca gctcttcccg gaggggcacg
     2641 tcatcgacct gcacccctgg gagtacaacg aggtgccggt cgtcctggcg gctgccctgg
     2701 cgaccgatgt gccgatcatc gccctgcacc tgacccggcc agcggtcgac attcccgacc
     2761 gcgcagtgct gggcatggcg tcgcacttcg aggcggctcg cggggcgtat ctgatccggg
     2821 atgtggcggc tggcacgccg tccgccggca cggtctacgt gcagggcacc gtcacgaccg
     2881 ccagcgtggt caaggcgctg ccgcagctgg atgagcgcgg catcaacgtg cggatcgtgg
     2941 cctgcatctc gccgcagctc ttccgacggc aggacgaggc ataccgggac ggcatctgct
     3001 ccgcctcgga caggtggagc ggcatggcca tcaccaaccg cgccttcaag ctcatgaccg
     3061 atttcgtcga agggccgatc gcgaaggagt actcgctgtc atcggattgg gacaatcgat
     3121 ggcgtaccgg cggcaccgtc gacgaggtca tggacgaggc ccacctgggg ccgggccaca
     3181 tcctcgaggc gatcgagcga tacgcctccg agcgcgagga gcggcatgcg cgacttcgag
     3241 agatcgtggc tagcctcgag cggctaggct gaagcaccac atgagcgaga cgcatccgca
     3301 cacgtacgag caccagcgtc ctcggcgccc gacgcacctc gcccggcgca ccaccccgcc
     3361 accggagcgc cctgcaccgc cgaacctgcg ctacctgctc tacgttgggc tggcattgac
     3421 cgccgtggtc gtcgcgctgg cggtgccgta cgtcatcggc tacctgctcc agctggtgac
     3481 cggctaggcc ttcgccgtcc agaccagcac ggtctcgccg gagaccttta tctcggggcg
     3541 caccagcggc ggcaggtcaa tcggataggc ctgctcgtcg ccccggtagc cgctgccgga
     3601 cgaccagccc tcgcttccat tggcgagctg accagccagg ttgaagctgg ccccgtggca
     3661 cgggcagcgc aggtccttcc actccg
//
LOCUS       DATCQR010000190         2907 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_134500, whole genome shotgun sequence.
ACCESSION   DATCQR010000190 DATCQR010000000
VERSION     DATCQR010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2907)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2907)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2907
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134500"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..747)
                     /locus_tag="VIN32_06395"
     CDS             complement(<1..747)
                     /locus_tag="VIN32_06395"
                     /inference="COORDINATES: protein motif:HMM:NF014090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HEY8922244.1"
                     /translation="MTPIDDAAARADMAAADFDSGWVRDHLADERRKASLLGEIREAI
                     FGAQDGLVSTLAVVSTVAGASADRVPVLIAGIATGLAGVFSMAAGEYMSSKSQREIFV
                     AQIAGEREEVSERPGEAEAEMAYMFEEDGLPRHEASSVAGVIAAHPDVLLKTMVEKEL
                     GLTGEHAEGSPFQGALVMGTSFGLGSIVPVLAYLILPIEAATWAAVVASAAVLFGIGV
                     LKSRWTRQPWLRSGLEILVLGAFAGIAGFLF"
     gene            902..1786
                     /locus_tag="VIN32_06400"
     CDS             902..1786
                     /locus_tag="VIN32_06400"
                     /inference="COORDINATES: protein motif:HMM:NF025616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HEY8922245.1"
                     /translation="MAILSLLVLAFAVAACSAASTAFQNVGEDLKGGGAVPGGQPADG
                     RDVGGLPAPGTVTGADGKQVASLADQKIVKTGEITIEVPDVAAALGRVRALAVELGGY
                     VGGSQSGTKDESATLTLRIPAARFDDTLSRLHKLGGVVVEATREDDVTTAVVDLEARL
                     ENLQASETQYRALIAKAVKVEDILAVQLRLDEVRGQIEQLQAQLKELNGLADLSTLTV
                     TLAPSALQQTAGSWDPGKTVSDAFAALVGAAQSVGNGVIWFAIVWLPALIVLGVVLLL
                     AWRLVPGLRNRTRSASTE"
     gene            complement(1791..2408)
                     /locus_tag="VIN32_06405"
     CDS             complement(1791..2408)
                     /locus_tag="VIN32_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013017613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEY8922246.1"
                     /translation="MTSRAATLADVAVPAIGSTEASRWLRAALLVGAGAALTAVSAQI
                     VWFAPWNPLVPYTFQTAAVLLVGTALGSRLGLAAMLLYVALGAAGLGVFADGSSGLAA
                     DGALRASVGYLGGFILAGALVGWLAERGWDRSPASTIGLMVFGNLVIYAVGVPVLIAI
                     TGLDLATALQVGALDFVPWDLIKVAAAAALLPLAWRALGSGDRRG"
     gene            2540..>2907
                     /locus_tag="VIN32_06410"
     CDS             2540..>2907
                     /locus_tag="VIN32_06410"
                     /inference="COORDINATES: protein motif:HMM:NF013178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase domain-containing
                     protein"
                     /protein_id="HEY8922247.1"
                     /translation="MRTSLTRESTTPTATERPASFLSSGVLALEGGTALRGRLRGAGG
                     PAEGDLIFSTAATGWGEILTDPSYAGQIVILTHPMVGNYRIDPNELESDRVHPRAVIV
                     TRLVTPPAGPGRTFEELLIEA"
BASE COUNT          483 a         1039 c          978 g          407 t
ORIGIN      
        1 gaacaagaat ccggcgatgc cggcgaaggc acccagcacc aggatctcga gccccgaacg
       61 cagccacggc tgccgcgtcc atcggctctt cagcacaccg attccgaaca gcaccgcggc
      121 gcttgcgacc accgcagccc aggttgcggc ctcgatcggc aggatcagat atgccagcac
      181 gggcacgatc gagccaaggc caaagctggt gcccatcacc agggccccct ggaacgggct
      241 cccctcagcg tgctcgccgg tgagcccaag ctccttctcg accatcgtct taaggagcac
      301 gtcgggatgc gccgcgatga cgcccgcgac cgatgacgcc tcgtgtcgtg gcaggccgtc
      361 ctcctcgaac atgtaggcca tctccgcctc ggcctcgccc gggcgctcag aaacctcttc
      421 acgttcgccc gcgatctgcg ccacgaagat ctcgcgctgg ctcttgctgc tcatgtactc
      481 gccggcggcc atgctgaaga cgcccgccag gccggtggcg atgccggcga tcagcacggg
      541 aacccggtcc gccgaggcgc cggcgacggt gcttaccacc gccagcgtgc tgaccagccc
      601 gtcctgcgcc ccgaagattg cctcgcggat ctcgcccaac aggctcgcct tgcgccgctc
      661 atcggccagg tggtctcgca cccagccgga atcgaagtca gcggccgcca tatcggctcg
      721 cgccgcggcg tcatcaatgg gtgtcatgac gtcgatcgta gccgaggagg ggaacgagcg
      781 agtcaccgcc cgcgtccgag tggtggacac ataacttcag cacctggagg acggaccgat
      841 gcccagcccc gctcggcgct ccccgcgacg ccaatccccg cgacgccact ccgcgcgatc
      901 attggcgatc ctgagcctgc ttgtcctggc gtttgcggtc gccgcgtgct cagcggcaag
      961 cactgccttc cagaacgtgg gcgaagacct caagggtggc ggtgccgtgc ccggcgggca
     1021 gcccgcggac ggccgcgacg tcgggggcct gccggcaccg gggaccgtga ccggggctga
     1081 cggaaagcag gtcgccagcc tcgccgacca gaagatcgtc aagaccgggg agatcaccat
     1141 cgaggtgccc gacgtcgccg ctgcgctggg tcgcgtgcgg gcgttggcgg tggagctcgg
     1201 cggctatgtg ggcggatcgc agtccggaac gaaggacgag tcagcgacgc tgaccctgcg
     1261 catccccgct gcacgattcg acgacaccct gtcgcgcctc cacaagctcg gcggcgtcgt
     1321 cgtcgaggcg acgcgggagg acgacgtgac caccgcggtg gtcgatctcg aggcgcgtct
     1381 ggagaacctc caggcatccg agacgcagta ccgagccctg atcgccaagg cggtcaaggt
     1441 cgaggacatc cttgccgtgc agcttcgcct ggacgaggtg cggggccaga tcgagcagct
     1501 gcaggctcag ctcaaggagc tgaacgggtt ggccgatctg tccaccctga ccgtcaccct
     1561 ggcgccgagc gcacttcagc agaccgccgg cagctgggat cccggcaaga ccgtgtctga
     1621 cgccttcgcg gcgctggtcg gtgctgccca gtcggtcggg aacggtgtga tctggttcgc
     1681 gatcgtctgg ctgccggccc tcatcgtgct gggggtcgtc ctgctgctgg cctggcggct
     1741 ggtgcccggg ctgcgaaacc ggacgcgatc ggcgtcgacc gagtaactgg ctagcctcgt
     1801 ctgtcgcccg acccaagtgc ccgccaggcc agtggcagca gcgctgccgc cgcggcgacc
     1861 ttgatcaggt cccacggcac gaagtcgagc gcacctacct gcaaagcggt ggccaggtcc
     1921 agcccggtga tggcaatcag gaccggcacg ccaacggcgt agatgacgag gttgccgaac
     1981 accatgagcc cgatcgtgga ggccggtgac cgatcccagc cgcgctccgc gagccagccg
     2041 acgagtgccc cggccaggat gaagccgccc aggtatccga cgctcgccct caacgccccg
     2101 tcggcggcaa gccccgacga cccatcggcg aagacgccga gtcccgcggc ccccagggcg
     2161 acataaagca gcatcgccgc caggccgagg cgcgacccga gggcggttcc caccaacagc
     2221 accgcggccg tctggaaggt atacggcacg agcgggttcc agggcgcgaa ccacacgatc
     2281 tgggcagaaa cggcggtgag cgcggcaccg gcgccgacaa ggagcgccgc gcgcagccaa
     2341 cgggacgcct cggttgagcc gatagccggg acagcgacat cggccagggt tgcggcgcgg
     2401 ctggtcatgg gcgggactcc tgctgatggt gtcgaggctg gcgatggtac cgcgccgatg
     2461 gccctcggat gcgccacgcc tgcgctacac tgcgaccgtc ctttaagccc ggcactgcga
     2521 ggccggaaag gagcgatcct tgcgcaccag cctgacccgc gaatccacga cgccaaccgc
     2581 caccgagcgg ccggcgtcgt tcttgtcgtc aggggtgctt gcgctcgagg gcggcacggc
     2641 cctccgcggt cgcctccgag gtgcgggagg ccccgccgag ggcgacctga tcttctcgac
     2701 cgccgccaca ggctggggcg agatcctgac cgatccctcg tacgccggcc agatcgtgat
     2761 tctcactcac ccgatggtgg gcaactaccg gatcgatccg aacgagctcg agtccgatcg
     2821 agtgcatccc agggccgtca tcgtcacccg gctggtgacg cccccggccg gcccgggccg
     2881 caccttcgag gagctgctga tcgaggc
//
LOCUS       DATCQR010000191         4701 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_181330, whole genome shotgun sequence.
ACCESSION   DATCQR010000191 DATCQR010000000
VERSION     DATCQR010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4701
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181330"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1171)
                     /locus_tag="VIN32_06415"
     CDS             complement(<1..1171)
                     /locus_tag="VIN32_06415"
                     /inference="COORDINATES: protein motif:HMM:NF025806.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division FtsA domain-containing protein"
                     /protein_id="HEY8922248.1"
                     /translation="MQDRLRSLVERLTRVTGGSAGGPPALVALDVGTEFAKALVLAIT
                     PDEEGRLVGSVRGSGRQRQGLTHMQSGTVSDIDAVVANCSRALNEAREMAGLHATSAV
                     IGIAGELVKGTTSNGAVRREAPHVPMTEKELQQIVEQVQIEALADAERRIAWESGVER
                     MDVRLVHAAVVELKIDGYPVSNPIGFTGAQLELSVFNAFAPMVHLGALQSVASQLGLK
                     LLGVIAEPYAVATCLDPGELGDAGAVFVDIGGGTTDVALVRHGGVSGTKMLALGGRAF
                     TKGLAERFALSFSRAESLKLAAAEGELPEQVDRATLAEALAEDAAVWLGGVELMISDL
                     AEGELLPGRVYLCGGGAELPQIAAALDGDEWWHRLPFARKPQVRPLSPADVVGLHD"
     gene            complement(1174..3801)
                     /gene="alaS"
                     /locus_tag="VIN32_06420"
     CDS             complement(1174..3801)
                     /gene="alaS"
                     /locus_tag="VIN32_06420"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEY8922249.1"
                     /translation="MPELQAAEIRERFQRFFEARGHTRVPPAPLLARDDPTLLFVNSG
                     MVQFKRVLTGEERRDYTRAVDAQPCLRVAGKHNDFEEVGRTPRHQTLFEMLGNWSFGD
                     YFKREAIHWAWEFLTVELGIPAERIAATTYTDDDEARRIWSDEIGLPPERMAQWGNIA
                     VGDDHNFWSMGETGPCGPCSELHYDRGAELSEGPECIPDHSETCPRWLEIWNLVFMQF
                     DRAADGTLTPLPFPSVDTGMGLERMASAVQGVDSNYRTDLFTPIIDRLAQHLGHDPQN
                     VESERFSYQVVADHSRAMTFLIGEGLKPSNEGPGYVLRRIMRRAVRHLRLMGIRQPVL
                     AETCGSVIEVMGGAYPYLVERRDEILAAVDVEERKFARTLEAGSERLAALVEAAGPEG
                     AISGDDAFRLHDTFGFPIDLTVEIAAERGVTVDREGFEAAMEEQRERSRGSRAGRFAP
                     QPGVEGLRSEFIGYPNETSADGIAVLLVVEGDGSAQVVLEKTPFYAEGGGQIGDRGEL
                     VGERGRLLVEDTQRAGEAIVHVGKLEGSLAAGDRVNARVDEERRSGAARNHTATHLLH
                     RALREVLGEQAKQAGSWVGPDGLRFDFPADAATPREAQRDVERRVNAQIRRNAVVTPT
                     EMSLAEAQGIGADMFFGEKYAPERVRVVRVDGYSAELCGGTHVAATGQIGSFRITGEA
                     SIGTGLRRIEAVSGAAADELVATRLDALHEAAQLLGAPEDQVASRIEALQARLREAEK
                     GGTKATGSRLDAAAALANAQVAGEAKVIVERYPDADGAALRVLADDLRAATGRFVAVV
                     AGDRGGPSILVGASRDLVGEGFDASAIVREVAPMIAGGGGGRAELAQAGGKDLAGLDE
                     ALREGARLALEALQRIENG"
     gene            complement(3891..4151)
                     /locus_tag="VIN32_06425"
     CDS             complement(3891..4151)
                     /locus_tag="VIN32_06425"
                     /inference="COORDINATES: protein motif:HMM:NF024143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEY8922250.1"
                     /translation="MKDSGFGSFLAGVVIGGLIGAAIGLLLAPRTGDETREQVGEFVG
                     EKRAAIGEAINEGRAAAEKARAEMMSAYDAGGGEPAPEGAAS"
     gene            complement(4190..4507)
                     /locus_tag="VIN32_06430"
     CDS             complement(4190..4507)
                     /locus_tag="VIN32_06430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8922251.1"
                     /translation="MTPWMVLTVFVAAINLMIFIALRGRWDRRLAILALASLLGTATG
                     NAIGARTGLELVRIGDFHLLAACLLAQLAMLATDLLAQLGPSRPPPERQARGSRDGER
                     TDR"
     gene            complement(4504..>4701)
                     /locus_tag="VIN32_06435"
     CDS             complement(4504..>4701)
                     /locus_tag="VIN32_06435"
                     /inference="COORDINATES: protein motif:HMM:NF042466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase beta-barrel
                     domain-containing protein"
                     /protein_id="HEY8922252.1"
                     /translation="PADRFEASVRIRHRGAEVPCEVTVVGDERLLVETSEPVWAPAPG
                     QAAVLYDGEECLGGGRIARAT"
BASE COUNT          693 a         1784 c         1513 g          711 t
ORIGIN      
        1 catcgtggag tcccaccacg tccgccgggg agagcggccg aacctggggc tttcgcgcaa
       61 agggaagccg atgccaccat tcgtcgccat ccagggcagc cgcgatctgg ggcagctccg
      121 cacccccgcc gcacaggtag acgcggccgg gcagcagctc accctccgcc aggtcgctga
      181 tcatcagctc cacgccaccc agccatacgg ccgcatcttc ggccagggcc tcggccagcg
      241 tcgcgcgatc gacctgctcg ggcagctcgc cctccgcggc cgccagcttc agtgactcgg
      301 cacgcgagaa ggagagcgca aagcgctcgg caagtccctt ggtgaatgcc ctgccgccga
      361 gcgccagcat cttcgtcccc gagacgcccc cgtggcggac cagcgccacg tcggtcgtcc
      421 ccccgccgat gtcgacgaag actgcgccgg catcgcccag ctcgccgggg tcgaggcagg
      481 tggccaccgc gtacggctcg gcgatcacgc cgagcagttt caggccgagc tggctcgcca
      541 cggattgcag cgctcccagg tgcaccatcg gcgcgaaggc attgaagacc gacagctcga
      601 gctgcgcccc ggtgaagccg atcgggttgc tgaccgggta gccgtcgatc ttgagctcga
      661 cgacggcggc atgcaccagc cgcacgtcca tgcgctcgac cccgctctcc caggcgatcc
      721 ggcgctccgc atcggccagc gcctcgatct gcacctgctc gacgatctgc tgcagctcct
      781 tctctgtcat gggcacgtgc ggagcttccc ggcgcaccgc tccattcgag gtcgtgccct
      841 tgaccagctc cccggcgatg ccgatcaccg ccgatgtcgc atgcaggccc gccatctctc
      901 gtgcctcatt gagggcgcgc gagcagttgg ccacgaccgc gtcgatgtca ctgaccgtcc
      961 ctgactgcat gtgagtcagt ccctggcgct gccttcccga gccgcggacc gatccgacca
     1021 gtcgcccctc ctcgtcgggc gtgatggcca gcacgagggc cttggcgaac tccgtcccga
     1081 catcgagcgc gaccagcgcc ggcggaccgc cagccgagcc cccagtgacc ctggtcaggc
     1141 gctcgacgag gcttctcagc cggtcctgca ccgtcagccg ttctcgatcc gctggagcgc
     1201 ctcgagcgca aggcgcgctc cctcgcgcag cgcctcgtca agtccggcga gatccttgcc
     1261 tccagcctgg gccagctccg cgcgaccgcc acccccacca gcgatcatcg gtgccacttc
     1321 gcggacgatc gccgaggcgt cgaagccttc cccgacgagg tcgcggctgg cgccgaccag
     1381 gatcgacggg ccgccgcgat cgccggcgac cactgccacg aagcgtccgg tcgccgcccg
     1441 caggtcgtcg gccaggacgc ggagcgcggc gccatccgcg tccggatagc gctcgacaat
     1501 caccttcgcc tcaccggcca cctgtgcatt ggccagggcc gccgcagcgt cgagccgcga
     1561 tccggtggcc ttggtgccgc ccttctccgc ctcccgcagg cgcgcctgca gcgcctcgat
     1621 ccgactcgca acctggtcct cgggcgcacc gagcagctgc gccgcctcgt gcagcgcatc
     1681 gagccgagtg gcgaccagct catcggccgc cgcgccgctg acggcctcga tgcgccgcaa
     1741 tccggtgccg atgctcgcct cgccggtgat ccggaaggag ccgatctgcc cggtggccgc
     1801 aacgtgggtg ccaccgcaca gctccgcgct gtagccgtcg acacggacca cgcgaacccg
     1861 ctcaggcgcg tacttctcgc cgaagaacat gtcggcgccg atcccctgcg cctcagccaa
     1921 tgacatctcg gtcggcgtca ctaccgcgtt gcgccggatc tgggcgttca cgcgacgctc
     1981 gacgtcgcgt tgcgcctcac ggggagtggc cgcatccgca ggaaagtcga accgaagccc
     2041 atcgggcccg acccagctgc ctgcctgctt ggcctgctcc ccgagcacct cgcggagcgc
     2101 acggtgcagc agatgcgtgg cggtgtggtt gcgcgccgcg cccgaacgcc gctcctcgtc
     2161 aacgcgggca ttgacacggt cgcccgccgc gagcgacccc tccagcttcc cgacatgcac
     2221 gatcgcctcg cccgcacgct gggtgtcctc gaccaggagc cggcctcgct caccgacgag
     2281 ttccccgcgg tcgccgatct ggccgccgcc ctccgcgtag aacggtgtct tctcgaggac
     2341 gacctgggcg gacccgtcgc cctccacaac cagcagcacg gcgatgccat ctgccgatgt
     2401 ctcattggga tagccgatga actcgctgcg caggccttcg accccgggct gcggcgcgaa
     2461 ccggccggcg cgggagcccc gcgaacgttc gcgctgttcc tccatggctg cctcgaaccc
     2521 ttcacggtcg acggtcacgc cacgctccgc ggcgatctcc acggtcaggt cgatcggaaa
     2581 gccgaaggtg tcgtggaggc gaaacgcatc gtcgccggag atcgcgccct ccggcccggc
     2641 ggcctcgacc agggcggcca ggcgctcgct ccccgcctcg agggtccgcg cgaacttgcg
     2701 ctcctccaca tcgaccgcgg ccaggatctc atcacgcctc tcgacgaggt aggggtaggc
     2761 gccgcccatc acctcgatga cgctcccgca tgtctcggcg agcaccggct gccggattcc
     2821 catcaggcgc aggtgccgca cggcgcgacg catgatccgg cgcaggacgt agcccggccc
     2881 ctcgttcgac ggcttcaggc cttcaccgat caggaatgtc atcgcccggc tgtggtcggc
     2941 gaccacctgg tagctgaagc gctcgctctc cacgttctgc ggatcgtggc ccaggtgctg
     3001 cgccagccgg tcgatgatcg gcgtgaacag gtcggtgcga tagttgctgt cgaccccctg
     3061 gacggcgctg gccatccgtt cgaggcccat cccggtgtcg acgctgggga acgggagggg
     3121 cgtcagcgtc ccgtcggccg ctcggtcgaa ctgcatgaag accaggttcc agatctcaag
     3181 ccagcggggg caggtctccg agtggtcagg gatgcactcc ggcccctccg aaagctccgc
     3241 gccgcggtcg tagtgcagct cgctgcacgg cccgcagggg ccggtctcgc ccatcgacca
     3301 gaagttatgg tcgtcgccca ctgcgatgtt gccccactgc gccatccgct cgggcgggag
     3361 cccgatctcg tcgctccaga tgcgccgtgc ctcgtcgtca tcggtatagg tggtggcagc
     3421 gattcgctcg gcggggatcc ccagctccac cgtcaggaac tcccacgccc agtggatcgc
     3481 ctcgcgcttg aaatagtcgc cgaagctcca gttgccgagc atctcgaaga gggtctggtg
     3541 gcgcggcgtg cggccgacct cttcgaagtc gttgtgcttg cccgccaccc gcaggcaggg
     3601 ctgggcgtcc acggcgcgcg tgtagtcgcg cctctcctcg ccggtcagca cgcgcttgaa
     3661 ctggaccatg cccgagttga cgaagagcag cgtggggtca tcgcgggcaa ggagcggggc
     3721 cggcggcaca cgggtgtggc cgcgcgcctc gaagaagcgc tggaagcgct cgcggatctc
     3781 ggcggcctga agctccggca tcggtcctgc cctgtgaccc ctaactcacc acgagagccg
     3841 gctacgaccg gctctcgact cctcgtgcgt gacttcgcgc gaatggccgg tcaggaggca
     3901 gcgccctccg gcgcaggctc cccgccgccg gcatcgtacg cggacatcat ctcggcgcgt
     3961 gccttctcgg ccgccgcgcg accctcgttg atggcctcgc cgatggctgc ccgcttctcg
     4021 ccaacgaact caccgacctg ttcgcgagtc tcgtcacccg ttcgaggggc aagcaggagc
     4081 ccgatcgccg cgccgatgag cccaccgatc acgacccctg cgaggaagct gccgaacccg
     4141 gaatccttca tggctagttg cccgtcccta gcgtttgcgt ccgctgattc taccgatccg
     4201 ttcgctcccc gtcccgacta ccgcgggcct gacgttctgg aggcggccgc gagggcccga
     4261 gctgcgccag cagatcggtg gccagcatgg cgagctgcgc gagcaggcag gcggcaagca
     4321 gatggaagtc gccgatgcgc accagctcga ggccggttcg cgccccgatc gcgtttcctg
     4381 tcgccgtgcc cagcagcgac gcaagggcca ggatcgccag ccgtctgtcc cagcgaccgc
     4441 ggagcgcaat gaagatcatc aggttgatcg ccgccacgaa gacggtgagc accatccagg
     4501 gcgtcatgtc gcgcgggcga tacgaccgcc gccgaggcac tcctctccgt cgtacaggac
     4561 cgcggcctgc cccggcgccg gtgcccacac cggctcggag gtctcgacca gcagccgctc
     4621 gtccccgacc acggtgacct cgcatggcac ctcagcgccg cggtgccgaa tgcggaccga
     4681 tgcctcgaag cgatcggcgg g
//
LOCUS       DATCQR010000192         1711 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_226982, whole genome shotgun sequence.
ACCESSION   DATCQR010000192 DATCQR010000000
VERSION     DATCQR010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1711
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226982"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="VIN32_06440"
     CDS             complement(<1..272)
                     /locus_tag="VIN32_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016471326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8922253.1"
                     /translation="MVEVAPGTIVVFSDIGCPWAHVAVWRLHDARRRLGLDGRVAFDH
                     RVFPLELFNSQPTPRDELAVEWEACAALTPRAGWQAWSAPDWAWPAT"
     gene            complement(277..1239)
                     /gene="deoC"
                     /locus_tag="VIN32_06445"
     CDS             complement(277..1239)
                     /gene="deoC"
                     /locus_tag="VIN32_06445"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010352563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEY8922254.1"
                     /translation="MATTTPARRRADRDLSAPRHQALGSLLGRVWAVDQVGVEERAAS
                     LAKRSIKRESKLWALDLAIRMVDLTTLEGKDTPGKIRALAGKARRPDPTDPSIPSVAA
                     LCVYPNMIATAKAALAGSSVKVVSVATYFPSGQAALAVKLDDVRQAVTAGADEIDMVI
                     DRGAFLSGHYAQVFDEIVAVKEATGDHHLKVILETGELETYDNVRRASVLAMAAGADF
                     IKTSTGKVQPAATLPVTLVMLEAIRDFYAQTGRAVGMKPAGGIRTAKEAISYLTVLYE
                     TLGPDWMTPDRFRFGASTLLNDLLMQVRKERTGRYSGGDYFTLD"
     gene            1308..1697
                     /locus_tag="VIN32_06450"
     CDS             1308..1697
                     /locus_tag="VIN32_06450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8922255.1"
                     /translation="MDFRPDPSHEILLYCLTRDHDLADAVEGRVRRALAFFYNDAARL
                     HQAVVLRSPDLVLVDTGAIRQEYGDAGLGPVVAFLRERAPAARIAVRPVPGTEWLIQA
                     EAGERVDLLPADREACVDAVGVLCGAA"
BASE COUNT          247 a          584 c          613 g          267 t
ORIGIN      
        1 gtcgccggcc acgcccaatc cggcgcgctc caggcctgcc agccggcgcg aggcgtgagc
       61 gcggcgcagg cctcccactc cacggccagc tcgtcgcggg gggtcggctg cgagttgaag
      121 agctccagcg ggaagacacg atggtcgaag gcgacgcgac cgtcgagtcc caggcgccgc
      181 cgcgcgtcat gcaggcgcca cacggcgacg tgcgcccaag ggcagccgat gtcgctgaag
      241 accacgatgg tgccgggagc aacctcgacc atcggctcag tcgagggtga aatagtcacc
      301 ccccgagtag cggccggtac gttccttgcg aacctgcatc agcaggtcgt tgaggagggt
      361 cgaggcgccg aagcggaacc tgtccggagt catccagtcc ggcccgagcg tctcgtacag
      421 caccgtcagg tacgagatgg cctccttggc ggtccggatg ccgccggcgg gcttcatgcc
      481 gactgcgcgc ccggtctgtg cgtagaagtc gcggatcgcc tcgagcatga ccagcgtcac
      541 cggcagggtc gccgcgggct gcaccttgcc ggtcgaggtc ttgatgaagt ccgctccagc
      601 ggccatggcc aggaccgatg cacgccgcac gttgtcgtag gtctccagct ccccggtctc
      661 gagaatcacc ttcaagtggt ggtcgccggt cgcctccttg acggccacga tctcgtcgaa
      721 gacctgggcg tagtggccgg acaggaaggc gccgcggtcg atcaccatgt cgatctcgtc
      781 cgcgcccgcc gtcaccgcct ggcgaacgtc atccagcttg accgcgagcg cagcctgtcc
      841 ggacgggaag taggtggcca ccgacacgac cttgacgctc gatccggcca gcgcggcctt
      901 ggcggtggcg atcatgttcg ggtagacgca cagggcagcc acgctgggga tgctcggatc
      961 agtgggatcg gggcggcggg ccttgccggc cagggcgcgg atcttgcccg gcgtgtcctt
     1021 cccctccagc gtggtcaggt cgaccatccg gatcgccagg tcgagcgccc acagcttcga
     1081 ctcgcgcttg atgctgcgct tggcgaggga cgcggcgcgc tcctcgaccc caacctgatc
     1141 caccgcccag acacgtccca ggaggctgcc gagcgcttgg tggcgcgggg cgctcaggtc
     1201 gcggtccgcg cgtcgccgcg ctggcgtggt ggtggccatg ggcccggaat tgtagcgccc
     1261 ggccccgatc aggccgatgc gcggtcgggt gataggcttc ggagacgatg gatttcaggc
     1321 cagacccgtc gcacgagatc ctgttgtact gcctgacccg ggaccacgac ctggcagacg
     1381 cggtcgaggg acgggtcagg cgtgcccttg ccttcttcta caacgatgcc gcgcgactgc
     1441 accaggcggt cgtgctgcgc tcccctgacc ttgtcctcgt cgacaccggc gcgatccggc
     1501 aggaatacgg cgatgcggga ctgggtccgg tggtcgcctt cctgcgcgag cgggcgccgg
     1561 cggcgcgcat cgccgtccgg cccgtgccgg ggaccgagtg gctgatccag gccgaggcgg
     1621 gcgagcgggt cgacctgctc cccgccgatc gggaggcgtg cgtcgatgcg gtgggcgtcc
     1681 tgtgcggcgc agcctgaggg cttactgact c
//
LOCUS       DATCQR010000193         4101 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_165797, whole genome shotgun sequence.
ACCESSION   DATCQR010000193 DATCQR010000000
VERSION     DATCQR010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4101
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165797"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            139..513
                     /locus_tag="VIN32_06455"
     CDS             139..513
                     /locus_tag="VIN32_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY8922256.1"
                     /translation="MAVRVLICDDQEAFRAAAREVVNATQGFEVVGESDTGEESVLAA
                     GLLHPDLVLMDVHLPGIDGLEASRRIREAYPKVAVLLLSTYDREEFAPRIAESGAIAF
                     ISKAAFGPDGLTEMWTRAAVVN"
     gene            756..1895
                     /locus_tag="VIN32_06460"
     CDS             756..1895
                     /locus_tag="VIN32_06460"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEY8922257.1"
                     /translation="MKGTRAIVAGAGIGGLAAGIALRRIGFEVTVLERSPAPREVGSG
                     IVIQPNGVRALEALGVPALAMSGLQGINLRTSGGRHLSETRIGSYRERYGSEVMVVAR
                     SDLHSLLIETLGRDRVTAASEVTGYEIGTGQVTVKLRDGRRLEADLLIGADGLRSAVR
                     AQMLEDGEPRYLGCTAWRATTDLAGSGLQGGQGLNWWGPGGEFGVLPMGRSVYWFATA
                     NAALGGVDPPGAARIQALLDRFGGWEAPIADVIASTPPDAILRNDLYDRPPARRWLDG
                     PVALLGDAAHPMAPNAAQGACQALEDAAALGAALAEGRPIDAGLAAYQRLRLNRANEL
                     VRLSRQISATVQTESRLLRNVRNLAARLMPERLLLSQMDRTLRGS"
     gene            complement(1931..2581)
                     /locus_tag="VIN32_06465"
     CDS             complement(1931..2581)
                     /locus_tag="VIN32_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016641679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY8922258.1"
                     /translation="MIRVLIADDHPLVRSGLKALLASVEGIEVVGEAADGGATVELAL
                     RERPDVVVMDIQMPGQDGIEATRRLAAEAPQVGVLVLTMHEDDESVFAAMRAGARGYV
                     LKGAEQEEIVGAIHAVSRGEAIFGPGVAQRVLRLFSQERPPAPFPELTARERETLDLM
                     AAGASNREIARKLFLSPKTVSNNVSYILGKLQVADRSKAIVRARRSGLGRDGSEAG"
     gene            complement(2578..>4101)
                     /locus_tag="VIN32_06470"
     CDS             complement(2578..>4101)
                     /locus_tag="VIN32_06470"
                     /inference="COORDINATES: protein motif:HMM:NF019350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEY8922259.1"
                     /translation="TTTFAASGPPPWAASLHNPAELWFGEQLAQVGLVVAGAGVAAAA
                     VSMLLRFMRSRGEERQQIKWLALAAVPVLASFGGFGNVWVSTVAVCIFVLPITLAILK
                     YRLYDLDLVVNRALVYGALSASVVAVYVAVVGAAGLLASGQRLNIVSVLATLAAALLV
                     LPLRQRLQRLVDRLLYGRRREPLSVVTTLARRLESAGTPEDLLRGAVSELADALKLDG
                     LEARLADGQIVASHGHTGADAVRTPLIHQGLTIGELVATPRRGESLSPRDQRFLQDLA
                     PQLAVALHALRLTVDLQRSRERLVFVQEEERRRIRRDLHDSLGPTLTAVAFQADAARN
                     LVERDPQTAIQLLGELRTEVSSAIAEIRRLVDGLRPPALDEVGLVEALRQQAGRFAEA
                     RQDGSLAVEVSAPGGVDGLPAAIEVAAYRIAAEAINNAARHAHASRCRVRLSAGEALE
                     VEVVDDGSGWQAGARAGVGLASMRERAEELGGTLVLSAAPGGGSRVLASIPLKARRP"
BASE COUNT          647 a         1468 c         1388 g          598 t
ORIGIN      
        1 tcccggacga tgaagctgtc gtccgcgagc acgaccctga tcggcatcgc acacaggata
       61 gggctggccc caccaattga aatggtgcag gcaccattgg gcgcgcgcag cctcagggct
      121 acgctgcgga aagagaacat ggccgtacga gtcttgatct gcgacgacca ggaagccttt
      181 cgcgccgcgg cgcgagaggt cgtcaacgcg acccagggct tcgaagtcgt gggtgagtca
      241 gacaccggcg aggagtcggt cctggctgcc ggcctgctgc accccgacct ggtcctgatg
      301 gacgtccatc tgcctggcat cgacgggctg gaggcaagcc gccgcatccg ggaggcttac
      361 ccgaaagtcg ccgttctgct cctctccacc tacgaccgcg aggaattcgc accacggatc
      421 gccgagtccg gcgccatcgc attcatctcg aaggccgcct ttggcccaga cggcctcacg
      481 gagatgtgga cccgcgccgc cgtcgtcaac tgagatggac cgggttacgc catgaaaaag
      541 gagcggaagg atgtgtcgtc acagtgcttt cggagcgcat cgacctagtg ctcattcgca
      601 acggcgtcac cgtgtcggat gccacccgga tcgggaacac gacagccgac cggacgcggt
      661 cgggattgtg gccctccgac catgctggtg tcgcagcgac gttgctgatg cccgacacac
      721 cttgagccgg agcacctgcg gctgagccga cagcaatgaa ggggacgcgg gcaatcgtgg
      781 ccggggctgg gatcggtggg cttgccgcgg gcatcgcgct gcgccggatc ggcttcgagg
      841 tcaccgtcct cgagcgttca ccggcgccgc gagaggtcgg ctccgggatc gtgatccagc
      901 ccaacggggt tcgcgccctg gaagccctcg gcgttcccgc cctggcgatg agcggcctgc
      961 agggcatcaa ccttcgcacc tcgggcggtc gccacctcag cgaaacgcgg atcggcagct
     1021 accgcgagcg ctacggctcg gaagtcatgg tcgtcgcgcg gagcgacttg cactcgctcc
     1081 tcatcgagac cctcggccga gatcgggtga ccgcggcgtc ggaggtgacc gggtacgaga
     1141 tcggcaccgg ccaggtgacg gtgaagcttc gcgacggcag gagactggag gcggacctgc
     1201 tgatcggggc ggatgggctg cgctcggccg tgcgcgccca gatgctcgag gatggggaac
     1261 cgcgctatct cggctgcacc gcgtggcgcg cgaccaccga cctggccggc agcggcttgc
     1321 agggtggcca gggactcaac tggtgggggc ccggtgggga gttcggcgtc ctgccgatgg
     1381 ggcgcagcgt ctactggttt gcgacggcca acgcggccct cggcggcgtt gatccgcctg
     1441 gtgctgcacg catccaggct ttgctcgacc gtttcggcgg ctgggaagcg ccgatcgccg
     1501 acgtcatcgc gagcaccccg cccgatgcca tcctccgcaa cgacctctac gatcgcccac
     1561 cggctcgccg ctggctcgat gggccggtgg cactgctggg cgatgccgct cacccgatgg
     1621 cccccaacgc tgcccaggga gcatgccagg cgctggagga cgcagccgcg cttggcgcgg
     1681 cgctggccga ggggcggccg atcgacgcgg gcctggccgc ctaccagcgc ttgcggttga
     1741 accgggccaa cgagctcgtc cgcctctccc gccagatctc ggcgaccgtc cagacggaga
     1801 gccggctctt gagaaacgtc cgcaacctgg cggcccgtct catgcccgag cgcctgctcc
     1861 tgagccagat ggaccgaacc ctgcgtggat cctgatcgat cgaaggttca gggttttcga
     1921 tgatttccgg tcagccggcc tcgctgccgt cgcgaccgag ccccgaccgt cgcgctcgca
     1981 cgatcgcttt cgatcggtct gccacctgga gcttgcccag gatgtaggag acgttgttgc
     2041 tcaccgtctt ggggctgaga aagagcttgc gagcgatctc ccggttgctg gccccagcgg
     2101 ccatcaggtc gagggtctcc cgctctcgag cggtcagttc ggggaagggc gcgggcggcc
     2161 tctcctggct gaacagccgg agcacccgct gggcgactcc cggaccaaag atcgcctccc
     2221 cgcggctcac cgcgtggatg gcccccacga tctcctcctg ctcagctccc ttgaggacgt
     2281 agccgcgagc tcccgcccgc atggccgcaa agaccgactc gtcgtcctcg tgcatggtca
     2341 ggacgagcac tcccacctga ggcgcctcgg cagccagccg gcgggtggct tcgatcccgt
     2401 cctggccggg catctggatg tccatcacga ccacgtccgg cctctcgcga agcgcgagct
     2461 cgaccgtcgc ccctccatcg gcggcttcgc ccacgacctc gatgccttcc accgaggcga
     2521 gcagcgcctt gagcccgctc cgcacgagag ggtggtcgtc agcgatgagg acgcggatca
     2581 cggccggcgc gccttcagcg ggatcgacgc cagcacccgg ctgccgccgc cgggagcggc
     2641 gctcaacaca agggtgccgc ccagctcctc ggctcgctcg cgcatcgagg ccaggcccac
     2701 gccggcccga gcgccggcct gccaacccga accatcgtcg accacctcga cctccagggc
     2761 ttcgccggcg ctcaaccgaa cgcggcagcg tgacgcatgt gcgtggcggg ccgcgttgtt
     2821 gatggcctcg gcggcgatgc ggtaggccgc cacctcgatc gccgccggca ggccatccac
     2881 accgcccggc gcactgacct caaccgcgag gctgccatcc tgccgtgcct cggcaaaccg
     2941 tccggcttgc tggcggaggg cctcaaccag gccgacctcg tcgagcgccg gcgggcgcag
     3001 cccatcgacg agcctccgga tctccgcgat ggccgagctc acctccgtcc gcagctcgcc
     3061 cagcagctgg atggcggtct gggggtccct ttcgaccagg ttgcgagccg cgtccgcctg
     3121 gaacgcgacc gcggtcaggg tgggcccgag gctgtcgtgc aggtcacgtc ggatgcggcg
     3181 ccgctcctcc tcctgcacga acaccagccg ttctcgcgac cgctgcaggt cgaccgtgag
     3241 ccggagagcg tggagcgcga ccgcaagctg cggcgccagg tcttgaagga atcgctggtc
     3301 gcggggggag agactttcgc cccggcgcgg tgtcgcgacc agctcgccga tcgtcaagcc
     3361 ctggtggatg agcggggttc gcaccgcgtc agcacccgta tgtccatggc tggcgacaat
     3421 ctgcccgtcg gccaaccggg cctcgaggcc atccagcttg agcgcgtccg ccaactcgct
     3481 caccgcgccg cgaaggaggt cttccggcgt gccggccgac tccagccggc gtgccagcgt
     3541 ggtcaccacg ctcagcggct cccgccgccg accgtagagc aggcggtcga ccaggcgctg
     3601 cagccgctgc cgcagcggca gcacgagcag ggcagccgcc agcgttgcca ggaccgacac
     3661 gatgttcagc cgctggccgg aggcgagcag cccggcggct ccgaccaccg ccacgtagac
     3721 ggccaccacc gatgcgctca aggcgccata gacgagcgcg cggttgacaa ccaggtccag
     3781 gtcatacagc cggtacttca ggatcgcgag cgtgatcggc aggacgaata tgcacaccgc
     3841 gacagtcgaa acccagacgt tgccgaagcc gccaaaactg gcaagcaccg gaaccgccgc
     3901 gagggccagc cacttgatct gctggcgctc ctccccacga gagcgcatga agcgcagcag
     3961 catcgagact gcagccgctg ccaccccggc cccggcgacc accaggccga cctgggccag
     4021 ctgctcgccg aaccacagct ccgccgggtt gtgcaggctc gccgcccacg gcggcggacc
     4081 ggaggccgcg aaggtcgtgg t
//
LOCUS       DATCQR010000194         6525 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_181527, whole genome shotgun sequence.
ACCESSION   DATCQR010000194 DATCQR010000000
VERSION     DATCQR010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6525
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181527"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="VIN32_06475"
     CDS             <1..399
                     /locus_tag="VIN32_06475"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEY8922260.1"
                     /translation="EIVLVRRRQPPLWALPKGTPNSGETLAETALRETSEEAGLLVEI
                     EEPISAITYFFVHGRTRFHKTVHFFLMRPIGGRLEDHDHEFDEVGWFQIDEALKLMTH
                     ATEREVVQRAAELLTARGGMAELSASGAPS"
     gene            396..1520
                     /gene="gcvT"
                     /locus_tag="VIN32_06480"
     CDS             396..1520
                     /gene="gcvT"
                     /locus_tag="VIN32_06480"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006346429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEY8922261.1"
                     /translation="MTEAPALLRHTPLHASHLALHARMVGYAGYEMPVQYTSIIDEHR
                     TVRSAVGLFDLSHMGEIHLSGPEALAFARYAVVSDPGLLEPGQAQYSMLCAPDGGIID
                     DVIVYRVEDGYLIVCNAANHDAVLAQLESLGAQGDFDVQVADRSDETALIAPQGPRAA
                     ELLGRLTDLDLSALGNYRSMQGRVAGTECLVARTGYTGEDGFELFCAARRAQRLWDAL
                     VSTGRDIGLQPCGLGSRDTLRLEAGMPLYGNELDRSVNPYEANLGRVVKLEKGEFVGR
                     GALAAVQQTGPQRKLVGLVMRDDAIARHGYPVLVDGAQAGVVTSGTLSPTLGERIAMA
                     YVPAADAAVGAALEVLVRERRHPAEQVKLPFYRRPKPDAA"
     gene            1554..1940
                     /gene="gcvH"
                     /locus_tag="VIN32_06485"
     CDS             1554..1940
                     /gene="gcvH"
                     /locus_tag="VIN32_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014397795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEY8922262.1"
                     /translation="MSTTPPDDRRYSREHEWVRMEGSVAVIGITGFAADELGDIVYVE
                     LPEVGATLAQFSSFGVVESVKAVSDLFAPVSGEVAEVNADLRTSPELLNTDPFGSGWI
                     AKIALGDPAEMDKLLDAAAYAELTAG"
     gene            1940..3283
                     /gene="gcvPA"
                     /locus_tag="VIN32_06490"
     CDS             1940..3283
                     /gene="gcvPA"
                     /locus_tag="VIN32_06490"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HEY8922263.1"
                     /translation="MSYSPHTAADRERMLAAIGVESVDDLFADIPAAVRSTRFDLPAP
                     LAEQEVRAELARLAGQNRIPRVSFLGAGVYRHLVPAVVSEVIGRAEFSTSYTPYQPEV
                     SQGTLQSIYEFQSLICELTGMEVATASHYDGATATAEAALMACRLTGRHKVAVSTAVN
                     PQVRRVLATYCAGPGVEVVEVPADLSEGGTGVTAPEVAAGALTDDCACLIAQQPNMFG
                     GIEAMGQLADAAHQVGALMVAVVEPTSLALLAAPGSYGADIVAAEGQPLGIPPSYGGP
                     YVGLMASRMDTVRQMPGRLVGATRDGQGRKGYVLTLQAREQHIRREKAASNICTNQAL
                     CALAAATYLSAVGPQGLREVADMSAVQARHAAAAIESAGLGQRRFSEPYFAEVAIRIS
                     DAARRHAALAEQGIVAGYLLQPDYPELADTLLLAATELTTDADVEALVAGLESTR"
     gene            3280..4959
                     /gene="gcvPB"
                     /locus_tag="VIN32_06495"
     CDS             3280..4959
                     /gene="gcvPB"
                     /locus_tag="VIN32_06495"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013074765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HEY8922264.1"
                     /translation="MSVLERPAAGDASGRGAAFTSPQAGDELTIVEPLIFDSSRPGRR
                     AVRYPSPSSAARAAAAGQPPIPDAAARATPPRLPEVAELDLLRHFNRLSHLNHAIELG
                     FYPLGSCTMKYNPKVNEWAARLPGLADSHPLDPDPLAQGSLELEWLLAELLKEISGFA
                     AISLQPAAGAHGELTGLLMTRAYHRSRGEGEQRTKVLVPDSSHGTNPATATMVGYTAV
                     TIPSNPRGGIDLEALRAALGPDTAALMITNPSTLGIFEDQIDQVVAAVKEAGAIAYMD
                     GANLNAILGRFRPGEAGFDVMHFNLHKTFSTPHGGGGPGAGPIGVSERLAPFLPTPLP
                     LLVAGEPAEVRRNAWTGRPTPGASFALEESGARPTSIGKVRAFTGNFGMFVRAYTYIR
                     SNGGDGLREVSNDAVLAANYLRVRLADAFDLPYDRICKHEVVFSGRRQKRQHGVTTLD
                     IAKAILDHRIHPPTIYFPLIVEEALMIEPTETESLETLDEFVEIMHGIAALAETAPDE
                     LKQAPRTTPVGRLDEAAAARRPDLKHDFAALSTPPSVQQEVGARPQAAGKG"
     gene            4998..5585
                     /locus_tag="VIN32_06500"
     CDS             4998..5585
                     /locus_tag="VIN32_06500"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEY8922265.1"
                     /translation="MVTEQPDGLVARMRLGDPDAFEEFFRTYERPVYITALAITRDPF
                     LAEEILQDCFVRAYRARHRLRTDRSPLPWLHRVTTNLCYSRIARRRMILEPITALISN
                     RLADLTSRPDQVVESREIIEVLQRAIDTLPPKQQTAVILYYLHGYSLAKSAEMAGCNV
                     GTMKSRVHYALKALRSRLPKERRTAAGARVSHSEL"
     gene            6311..>6525
                     /locus_tag="VIN32_06505"
     CDS             6311..>6525
                     /locus_tag="VIN32_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017604849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8922266.1"
                     /translation="MTAVQETAAGFQGAALTGGRSLRQIIWARLRRDKVAMICLVVLV
                     LFYLIGIFGPVVFGAFGLNPYTFDSAA"
BASE COUNT         1009 a         2300 c         2222 g          994 t
ORIGIN      
        1 gagatcgtcc tggttcgccg tcggcagccg ccactttggg cccttccaaa gggaacgccc
       61 aactcaggcg agaccctggc cgagacggca cttcgcgaga cgagcgagga ggccggcctc
      121 ctggtcgaga tcgaggagcc gatcagtgcc atcacctact tcttcgtcca tggccggacg
      181 cggttccaca agaccgtcca cttcttcctg atgcgtccga tcggcggccg cctggaggac
      241 cacgaccacg aattcgacga ggtcggctgg ttccagatcg acgaggcgct gaagctgatg
      301 acccacgcca cggagcgtga ggtcgtgcag cgcgcggccg agctgctcac ggcgcgcgga
      361 gggatggcgg agctgtcggc cagcggggca ccgtcatgac cgaggccccc gcgctcctgc
      421 gccacacgcc gctgcatgcc agccacctgg cgctgcacgc acggatggtt ggctacgccg
      481 gctacgagat gccggtccag tacacctcga tcatcgacga gcaccgcacc gtgcgcagtg
      541 ccgtcggcct cttcgacctg tcgcacatgg gagagatcca cctgtcggga cccgaggcgc
      601 tcgcctttgc gcgctatgcg gtcgtcagcg accccggcct gctcgagccg ggccaggccc
      661 agtactcgat gctgtgcgct cccgacggcg ggatcatcga cgacgtgatc gtgtaccggg
      721 tcgaggacgg ctacctgatc gtgtgcaacg ccgccaatca cgacgcggtg ctggcgcagc
      781 tggagtccct cggcgcccag ggcgacttcg atgtgcaggt ggcggaccgc tcggacgaga
      841 ccgcactgat cgcaccccag ggaccgaggg ccgcggagct gctgggccgg ctcaccgatc
      901 tcgatctctc cgcgcttggt aactatcgct cgatgcaggg cagggtcgcc ggcaccgagt
      961 gcctggtggc ccgcacgggc tacaccgggg aggacggctt cgagctgttc tgcgccgcgc
     1021 gccgcgccca gcgcctgtgg gacgcgctcg tctcgacggg cagggacatc gggctgcaac
     1081 cctgtgggct tggcagccgc gacacgctcc gcctggaggc ggggatgccg ctgtacggca
     1141 acgagctgga ccgatcggtc aacccgtacg aggccaacct cggccgggtg gtcaagctgg
     1201 agaagggcga attcgtcggg cgcggagccc tggccgcggt gcagcagacc ggtccacagc
     1261 ggaagctggt cgggcttgtg atgcgggacg acgccatcgc ccgtcacggc tacccggtgc
     1321 tggtcgacgg ggcgcaagcc ggggtggtga cgagcggaac gctctcgccg accctcggcg
     1381 agcggatcgc catggcctac gtccccgccg cggacgcagc cgtcggtgcc gcgctcgagg
     1441 tgctcgtccg tgagcgccgg cacccagccg agcaggtaaa gttgccgttc taccgccgcc
     1501 caaagccgga cgctgcctga cgcgcgacgg ccgcacaacc tcgaaggagc ccgatgtcca
     1561 cgaccccgcc agacgaccgg cgctactccc gtgagcacga gtgggtgcgc atggagggct
     1621 ccgtggccgt catcggcatc accggcttcg cggccgacga gctcggcgac atcgtctatg
     1681 tcgagctgcc ggaggtcggg gcaaccctgg cgcaattctc gagctttggc gtggtcgaga
     1741 gcgtcaaggc ggtgagcgac ctgttcgcac cggtctcggg cgaggtggcc gaggtcaacg
     1801 ccgaccttcg caccagcccc gagctgctca acactgatcc gttcgggagc ggctggatcg
     1861 ctaagatcgc cctcggtgac cccgcggaga tggacaagct cctggacgcg gccgcctatg
     1921 cggagctcac cgccggctga tgtcctactc cccgcacacc gctgccgacc gcgagcggat
     1981 gcttgcggcg atcggggtcg agtcggtcga cgacctcttc gcggacattc ccgccgccgt
     2041 gcgctccacc cgcttcgacc tgcccgcgcc attggccgag caggaggttc gtgccgagct
     2101 ggcgcgcctg gccggccaga accgaatccc gcgcgtcagc ttcctgggcg ccggcgtcta
     2161 ccgccacctg gtcccggcgg tggtcagcga agtaatcgga cgcgctgagt tctccacctc
     2221 gtatacgccg tatcagccgg aggtcagcca ggggaccctt cagagcatct acgagttcca
     2281 gtcactgatc tgcgagctga ccgggatgga ggtcgcaacc gcttcgcatt acgatggcgc
     2341 gaccgccacg gccgaggcgg cgctgatggc ctgccgcctg accggtcgcc acaaggttgc
     2401 cgtctcgact gcggtgaacc ctcaggtgcg ccgcgtgctc gccacctact gcgccgggcc
     2461 cggtgtcgag gtcgtggaag tgcccgccga cctgtcggag ggcgggaccg gcgtgactgc
     2521 gccggaggtg gccgctggcg cgctgaccga cgactgcgca tgcctgatcg cgcagcagcc
     2581 caacatgttc ggcgggatcg aggcgatggg gcagctggcc gacgcagcgc accaggtcgg
     2641 cgccctgatg gtggcggtcg tggagccgac gtcgctggca ctgctcgccg caccgggttc
     2701 ctacggcgcc gacatcgtcg ccgcagaggg gcagccgctc ggtatcccgc catcgtatgg
     2761 cgggccatac gtagggctca tggcctcccg catggatacc gtcaggcaga tgcccggccg
     2821 cctggtgggc gcaacccgag acgggcaggg gcgcaagggc tacgtgctca ccctgcaggc
     2881 ccgcgagcag cacatccgcc gtgagaaggc ggcgagcaac atctgcacca accaggcgct
     2941 ctgcgcactg gccgcggcga cctacctgtc ggcggttggc ccccaggggc tgcgcgaggt
     3001 ggcggacatg tcggcggtcc aggcgcgcca cgcggcagcg gcgatcgagt cggccggcct
     3061 cggccagcgg cgcttcagcg agccgtactt cgccgaggtg gcgatccgca tttccgacgc
     3121 cgctcggcgg cacgccgccc tcgccgagca ggggatcgtg gcgggctatc tcctccagcc
     3181 ggactacccc gagctggccg atacgctgct gctggccgcc accgagctga ccaccgatgc
     3241 cgatgtcgaa gccctcgtcg cggggctgga gtccacccga tgagcgtgct tgagcggccg
     3301 gcagcgggcg acgcctcggg tcgcggcgcg gccttcacct cgccccaggc gggggatgag
     3361 ctgacgatcg tcgagccgct catctttgac tcctcgcgac cgggccgccg cgccgtccgc
     3421 tatccgtccc cgtccagcgc cgcgcgggcc gccgcggccg ggcagccgcc gatccccgat
     3481 gccgccgccc gtgccacgcc cccacgcctg ccggaagtcg ccgagcttga cctgctgcgc
     3541 cacttcaatc ggctcagcca cctgaaccac gccatcgagc tgggcttcta tccgctcggc
     3601 tcatgcacca tgaagtacaa ccccaaggtc aacgaatggg cggcccgtct ccccggcctg
     3661 gccgattcgc acccgctcga ccccgatccg ctggcgcagg gcagcctgga gctcgagtgg
     3721 ctgctggccg agctcctcaa ggagatcagc ggcttcgcgg ccatcagcct gcagcccgct
     3781 gccggcgccc acggtgagct gaccgggctc ctgatgacgc gggcctacca tcggtcgcgc
     3841 ggggaaggcg agcagcgcac caaggtgctc gtccccgaca gctcgcatgg cacgaacccg
     3901 gccacggcca cgatggtcgg ctacacggcc gtcacgatcc cgtccaaccc ccgtggaggg
     3961 atcgatctcg aggcgctgcg cgcggcgctc ggcccggaca ccgcggcgct gatgatcacc
     4021 aacccctcga ccctgggcat cttcgaggac cagatcgacc aggtggtggc ggccgtcaag
     4081 gaggctggcg ccatcgccta catggatggg gccaacctga acgccatcct cggccgcttc
     4141 cggcccggcg aggccggctt cgacgtgatg cacttcaacc tgcacaagac cttctcgacg
     4201 ccccacggcg gcggcggtcc gggggccgga ccgatcgggg tcagcgagcg cctggcgcca
     4261 ttcctgccca ccccgctgcc gctgctggtg gccggtgagc ccgcggaggt ccgccgcaac
     4321 gcctggaccg gccgtccgac cccgggggcg agcttcgcgc tggaggagtc cggagccagg
     4381 cccaccagca tcggcaaggt gcgcgccttc accggcaact tcggcatgtt cgtgcgtgcc
     4441 tatacctata tccgcagcaa cggcggtgac gggctgcgcg aggtgagcaa cgatgcggtg
     4501 ctggcggcca actacctgcg cgtccgactg gccgatgcat tcgacctgcc ctacgaccgt
     4561 atctgcaagc atgaggtcgt cttctccggg cggcgccaga agcggcagca tggggtcacg
     4621 accctggaca tcgccaaggc gatcctcgat caccggatcc atccacccac gatctatttc
     4681 ccattgatcg tggaggaggc gctcatgatc gagccgaccg agacggagtc gcttgagacg
     4741 ctggatgaat tcgtcgagat catgcacggc atcgcggcgc tggctgagac ggcccccgac
     4801 gagctcaagc aggcgccgcg caccacgccg gtcggccgcc tcgacgaggc agccgcggcg
     4861 cgccggcccg atctgaagca cgacttcgcc gccctgtcga cccctccatc ggtccagcag
     4921 gaggttggtg ctcgacccca ggcggcggga aaggggtagg attccgcacc agtcggcgca
     4981 tcgtacgcgg agtacaggtg gtcactgagc agccggacgg gctagtcgcg cgcatgaggt
     5041 tgggggatcc cgacgcgttc gaggagttct tccgcacgta tgagcggccg gtctacatca
     5101 ccgcgctggc gatcacccgc gacccgttcc tggccgagga gatcctgcag gactgcttcg
     5161 tcagggccta ccgtgccagg catcggctgc gaaccgatcg gtctccgctg ccgtggctcc
     5221 atcgcgtgac caccaacctg tgctacagcc gtatcgctcg ccgcaggatg atcctggagc
     5281 cgataaccgc gctgatcagc aaccggctgg cggacctgac ctcgagaccg gaccaggtgg
     5341 tcgagtcccg cgagatcatc gaggtgctcc agcgcgccat cgacacgctg cccccgaagc
     5401 agcagaccgc cgtcatcctc tactacctgc atggctactc gcttgccaaa tcggctgaga
     5461 tggcgggttg caacgtgggc accatgaagt cgcgcgtgca ctacgcgctc aaggcgctgc
     5521 gatcgcggct cccgaaggag cggcgcacgg cggccggtgc aagggtgtcc cacagtgagc
     5581 tgtagccagg ctcgtcgcca gctgctggag cagttcgccc tgggcgagga gctcacgcct
     5641 cgctctgggc cgcaccttgc ccacctggag tcctgcgcgg actgtcgacg ggagatgggc
     5701 atcgatcgcg agctggtcga gagcctgcgt cgtgcactcg gcgagcgggt ggagggcctc
     5761 gctccgtccg aggccagctg ggggctggtc cgccgtcgga cggttgaccg cccggtgcgg
     5821 tcctgggcgg tccgggtgat gcactggaga ggaatggtgt ccgccgccgc agccgccggc
     5881 ttcatgattt tcgccgttgc cacggccccg gataccaggc tcctgcccat gagtcagtcg
     5941 ccgttcgtcg ccgctgcggc aaggcgggcc gtcccgcccc tcgaggacgc gagagactgg
     6001 cctccggagc tctcgagcgc agacgttgcg ccccaggcgt accccccgct gccgggctgg
     6061 cccatgcgga cctcgacaga gacagcgacg cgcgacgccg aaccgcagat ccccggacgt
     6121 atgcgctgag ggccgcgctt cgggaatgga gttgaacctg gttggctacg gtgaagtaat
     6181 agagatgagg gtcggtcagc gcgatcaaat gcgtacttga ccgaatcgtg cctcgcgctc
     6241 gaagatgcgc aagcaccagc agatacggat tcgtctccat agccgttcta cgagggatac
     6301 tctcccaacc atgacagcgg ttcaggaaac ggccgccggc ttccagggcg ctgccctcac
     6361 gggcggccgg agcctgcgcc agatcatctg ggcgcgtctc cgtcgcgaca aggtcgcgat
     6421 gatctgcctg gtggtcctgg tgctcttcta cctcatcggg atcttcggtc ccgtcgtctt
     6481 cggtgcgttc ggcctcaatc cgtacacgtt cgacagcgcc gcgat
//
LOCUS       DATCQR010000195        14678 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_26440, whole genome shotgun sequence.
ACCESSION   DATCQR010000195 DATCQR010000000
VERSION     DATCQR010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 14678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14678
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26440"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="VIN32_06510"
     CDS             <1..213
                     /locus_tag="VIN32_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEY8922267.1"
                     /translation="ADGPMQGLLGFTAEPLVSMDFKGDERSSIVDSLSTMVVGDNLLK
                     VIAWYDNEWGYSCRVADLASFMAARL"
     gene            219..1424
                     /locus_tag="VIN32_06515"
     CDS             219..1424
                     /locus_tag="VIN32_06515"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012615945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEY8922268.1"
                     /translation="MNKQTVRDIDVRGKRVFLRADLNVPLDDGRITDDTRIRASLPTI
                     RYLLERGAAVILASHLGRPKGKVNDALRLKPVADRLGQLLGRNVRMTGDALGPGVQVA
                     IGKLRPGDLLLLENLRFHGEEEANDPGFAKALADMAEIYVDDAFGSAHRAHASTEGIT
                     HFLPSVAGLLLEQEVTALSNLLAKPPKPFHTIIGGAKISGKLEVLEVLLARCQAVLVG
                     GGMANTFLAAKGYAMGKSLVETEQLGNAERIMSEARRKRVRLMLPTDLVIAAQIHPRS
                     QRQVVPLNGVPKDWTVVDIGPETVKTYTTHLAKARTIFWNGPMGIFEIAPFAEGTDAI
                     ANYVASRTADGVITVVGGGDSVSAVDKLGLTNQMSHVSTGGGASLEFVEGKKLPGVEA
                     LPDRDAPAK"
     gene            1421..2707
                     /gene="eno"
                     /locus_tag="VIN32_06520"
     CDS             1421..2707
                     /gene="eno"
                     /locus_tag="VIN32_06520"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015942420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEY8922269.1"
                     /translation="MTTGIAGVQGREVLDSRGNPTIEVEVRLDGGVRGRAIVPSGAST
                     GVHEAVELRDGDSARYGGKGVLRAVGNVNGEIAQAVAGRDALDQPGLDRALIDLDGSP
                     NKGRLGANALLGVSLATAHAAAAAQGQPLYRYLGDDAHVLPVPLVNILNGGKHAQDST
                     DFQEFMIAPLGAPTFAEALRWAAETFHALGELLHQRGFATTVGDEGGYAPSLATNEDA
                     IAVVLEAIQRAGYRPGEQIAIALDPAATEVYSEGRYVLTREKRTLTSDDLITFWADWV
                     GRYPIVSLEDGLAEDDWAGWTALTERLGDRIQVMGDDLLVTNTERLARAIREKAANSI
                     LIKLNQIGTLTETREAVEMAQAAGWRAIVSHRSGETEDTTIADLVVALGTGQIKTGSV
                     SRGERIAKYNRLLRIEEELGEAATYAGRGAFAALAV"
     gene            2707..3483
                     /locus_tag="VIN32_06525"
     CDS             2707..3483
                     /locus_tag="VIN32_06525"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020462430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY8922270.1"
                     /translation="MDGLTDHAQRNRAFWNELADTYQADHGPDLARHGAAWGVWQIPE
                     DEIHALGDVAGKDVLELGCGAAQWSIVLAQRGARPVGLDLSERQLGHARELMAAAGLD
                     FPLVHASAEAVPYPDASFDVIFCDHGAMVFADPRRTVPETARLLRDDGLLVFSMHTPI
                     ADICWPPDSEQVTDQLRLNYYELNVVDVGESEHVEFQLPYGEWIRLFRANGFEIEDLI
                     ELRPEPDAVSTYRTEEDREWARKWPMEHIWKVRRRREVAS"
     gene            complement(3480..3953)
                     /locus_tag="VIN32_06530"
     CDS             complement(3480..3953)
                     /locus_tag="VIN32_06530"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HEY8922271.1"
                     /translation="MDEPQAGRVVSVNVNQGGVPKLPVDEQWVGAMGLADDRHIEPEP
                     SHGGVDQAVCLYSMEAIQRLVAEGHNAFPGAFGENLTLEGIEMESLAGGDRLVIGEGG
                     LEIEMTWNAAPCTKQAQWFSDGRFARISHKTHPNEARWYARVVSEGPVRPGDAVS"
     gene            complement(4230..4505)
                     /gene="rpsT"
                     /locus_tag="VIN32_06535"
                     /pseudo
     CDS             complement(4230..4505)
                     /gene="rpsT"
                     /locus_tag="VIN32_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941688.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
     gene            4867..6678
                     /gene="lepA"
                     /locus_tag="VIN32_06540"
     CDS             4867..6678
                     /gene="lepA"
                     /locus_tag="VIN32_06540"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HEY8922272.1"
                     /translation="MIPPERTRNFSIVAHIDHGKSTLADRLLEATHTIDPRQMTSQVL
                     DSMDLEREKGITIKARAVRLQYDAKDGQTYALNLIDTPGHVDFTYEVSRSLQACEGAI
                     LVVDASQGIEAQTLANMHLALEQHLVIIPVINKIDLASADPEMVMAELEETLAIPREE
                     VILASAKEGKGIDDILEAIVTRIPPPSGEASAPLQALVFDSHYDPYKGVVAYVRVANG
                     TLHDHERIRFMATGIETDILELGYFRPQPVPTRRLTVGEVGYVASGLKSIADARVGDT
                     LTDADRPAAEPLPGYKQALPLVFAGIYPMRGDEYPLLRDPLEKLHLNDASFVYEPESS
                     VALGFGYRCGFLGLLHMEIVQERLEREFGLELIASAPSVEYRVKRLNRADELVVDNPA
                     RFPPAGEVEAISEPWVSATVITPTDYIGPIMELATTRRGDFINMEYLDPKRVNLHFEM
                     PLAELIIDFYDQLKSRSSGYASLDYAYLGYRPGELVKLDIMVSGEPVDALSLIVHRSN
                     AFRVGKSLVEKLKELIPRQMFEVPVQAAIGSNIISRETIRAMRKNVLAKCYGGDITRK
                     RKLLEKQKEGKQRMKRIGSVEIPQEAFLAILRMNEGQ"
     gene            6680..7348
                     /locus_tag="VIN32_06545"
     CDS             6680..7348
                     /locus_tag="VIN32_06545"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEY8922273.1"
                     /translation="MNFQDSLRLSIALGLTIFLVLLRFDSDRIMRSDYFRSRSGWVGP
                     LSYYGLVVLFAIGIAIILPRGRAQLFLAPGDPGSMLPVMLLFSAVAILNGLVLAFVRY
                     RAILPLPNQLLVSRLAGAAANAFAEELQFRSIVLGMLLFAGLPGGVGTALVVQALVYG
                     LAHRRLWKERDWYFLAGSVILGYAAGLATVTTGSVVPAMVGHFAVTASLFAFAGGRLR
                     QRPI"
     gene            7750..8091
                     /locus_tag="VIN32_06550"
     CDS             7750..8091
                     /locus_tag="VIN32_06550"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY8922274.1"
                     /translation="MAAAKVIHVEITGKDVPALQKFYSDIFDWKLETDNPEGYGMNRD
                     ESGFTAGIGGTQDGGAGQVTFYVHSDDPAGVLRRVEERGGKVIMPLTEVANDTTIALF
                     ADPEGHVVGIM"
     gene            8149..9303
                     /gene="hemW"
                     /locus_tag="VIN32_06555"
     CDS             8149..9303
                     /gene="hemW"
                     /locus_tag="VIN32_06555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HEY8922275.1"
                     /translation="MSLPTHHLYVHVPFCRLVCAYCDFVTVGGRSAEIARYPDLLLAE
                     LALRPASGQLATIYFGGGTPSLLPADGVALVVGAAIDRWGTAPTEVTLEANPSSRETP
                     DWSALGAAGVNRISLGIQSLRDPELLSLARGQTVAEGLAAYAAVRKAGFENVSIDLIY
                     GIPGQSLADWRHGLRLALALAPEHLSLYALQLAMAPDEWAAPPRPGALRWRQRMVARQ
                     DDRLAAAQYQVAEEVLEVAGFRHYELSSWARPGFESRHNSAYWERRPYTGIGAGAHSF
                     DGRTRSWNERDLDRYTARTSAGERAVAGSEELDEPTRAFEAVALGLRRVDGFSRSTFA
                     AEFGADPLVRFASAVAEGTAAELLEVTDDAIRLTARGRLFANEALVAFAP"
     gene            9623..10729
                     /gene="hrcA"
                     /locus_tag="VIN32_06560"
     CDS             9623..10729
                     /gene="hrcA"
                     /locus_tag="VIN32_06560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEY8922276.1"
                     /translation="MVEGMARDEERAPSRSVLDLTERQRIVLRAVVEDYVLNAIPVGS
                     QALVERYGLPVSSATVRSAMAELEALGLLSHPHTSAGRVPSDLGYRTYVESLMRESAL
                     DRADQLMIRHQFSQVQLTSNEWLRLAASILATSTRSAAVVTPARARRARFGHLQLVEL
                     ADRARLAVLVFGDGNVVQRRLHRTSLEKATPGEPAGQAELDTAAATLNRELAGLSAPQ
                     VRRKLGKLTPLAAHVAEVVAQMLEESDAVAVEEVFTDGIVNVLEQPEFAGGAKLRGVL
                     EVLQRSDFLEQLVPVLSRRGGVTVIIGHENVNDAMHEVSLVFAPYGTEDSALGLLGVL
                     GPTRMPYPRAIPTVRYLSTLMNELITNHPTENHD"
     gene            10722..11276
                     /locus_tag="VIN32_06565"
     CDS             10722..11276
                     /locus_tag="VIN32_06565"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEY8922277.1"
                     /translation="MTERPTNGAPEDTRHARGPSRAALIEKLEDMQRVLEETREQSDE
                     HLRGLQRTAADFANYRRRMDEEREGLSQFSNALLIGKLLAVLDDFDRALDTVPPGTNE
                     GWVDGVRLVERKLRGLLEAEGVREIDALGQPFDPNLHEAVVYEETADHPDNQVIGELQ
                     RGYRLGDRVLRPSLVRVANNPKER"
     gene            11281..13236
                     /gene="dnaK"
                     /locus_tag="VIN32_06570"
     CDS             11281..13236
                     /gene="dnaK"
                     /locus_tag="VIN32_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEY8922278.1"
                     /translation="MGKIIGIDLGTTNSVVAVMEGGEPQVITNAEGGRITPSVVAFAK
                     SGERLVGQVAKRQAITNPENTIFSIKRFMGRRFEDPEVAHSKDLVPYQVEKDPKSDGV
                     VVTLANGKTFSPPEISAMILQKLKSDAEAYLGEKVTEAVITVPAYFDDTQRQATKDAG
                     RIAGLDVKRIVNEPTASALAYGLDKTKEEKIAVYDLGGGTFDISILELSEGVFEVRST
                     NGDTHLGGDDFDQRVIDWLSEEFKKDQGVDLSKDRIALQRLKEAAEKAKIELSSTTTT
                     EINLPFISADASGPKHLVISLTRAKLEDLVADLVSKTSGPVKQALADASLKPTDIDEV
                     ILVGGMTRMPAVIEAVKEMFAGKEPHKGVNPDEVVAVGAAIQAGVLGGEVKDVLLLDV
                     TPLSLGIETLGGVATKMINRNTTIPTSHTQIFSTASDSQPSVDIVVLQGEREMASDNK
                     KLATFRLDGIPPAPRGVPQIEVTFDIDANGILNVAAKDKATNKEQKVTITASSMLAKE
                     DVDKMVRDAAEHAEEDRKRRAEIESRNEADALTYQAERLIKDMGDKLSDDDRKEINDR
                     IEAVREALKGTDAGVVDSTKQQLAEVLQKIGTRAYEAAGTPEPGGEDGSGGEGGEGGE
                     GGEGGGPTDGAAAEEEGETIEGEYKEV"
     gene            complement(13485..14261)
                     /locus_tag="VIN32_06575"
     CDS             complement(13485..14261)
                     /locus_tag="VIN32_06575"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY8922279.1"
                     /translation="MNDGELGGIVRALRHRRGWRQSDLARKADVSASLVGVLERGHAE
                     TLSVRSLRRIGSALDLRLGWDAGYRGAELARLRDAGHAQLAEWLTRQLEAFAWTVAPE
                     ASFNHYGDRGRIDLLALHPATRSLVVVEVKTVIAEIQDLLGTLNTKERVAPTVAQSLG
                     WRAAGAVPFLLVADGTTNRRRLADHSRLFARFGLRGRGALAWIRQPSGTPSGLLLLTK
                     LPDRSGVGLRRAGRQRVRVQQPAKSVNRSDNRGANGLETT"
     gene            14485..>14678
                     /locus_tag="VIN32_06580"
     CDS             14485..>14678
                     /locus_tag="VIN32_06580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8922280.1"
                     /translation="MIKIWSELRVARLKEQVADLATLVWVVFWGNLVWQLFQLLTGFA
                     QAGRTIHSGGKAMIQSGVDIG"
BASE COUNT         2480 a         5000 c         4930 g         2268 t
ORIGIN      
        1 gcggacggac cgatgcaggg gctgctcggc ttcaccgccg agccgctggt gagcatggac
       61 ttcaagggtg acgagcgatc cagcatcgtc gacagcctct cgaccatggt ggtgggcgac
      121 aacctgctga aggtcatcgc ctggtacgac aacgagtggg gctactcctg ccgcgtggcg
      181 gacctggcca gcttcatggc cgcccggctg tagcctgcat gaacaagcag acggttcggg
      241 atatcgacgt tcggggcaag cgcgtcttcc tgcgcgccga tctcaacgtc ccgctcgacg
      301 acgggcggat caccgacgac acccgcatcc gcgccagcct gcccactatc cgctacctcc
      361 ttgaacgggg cgccgcggtg atcctggcca gccaccttgg ccggccgaag ggcaaggtca
      421 acgacgcgct gcgcctcaag cccgtggcgg atcggctggg ccagctgctg ggccgcaacg
      481 tgcgcatgac cggcgacgcg cttggtccgg gcgtccaggt ggcgatcggc aagctgcgcc
      541 ctggcgacct gctgctgctc gagaacctgc gcttccatgg cgaggaggag gcgaacgacc
      601 ctgggttcgc caaggcgctc gccgacatgg ccgagatcta cgtcgacgac gcctttggct
      661 cggcgcatcg ggcccatgcc tcgacagagg ggatcaccca cttcctgccg agcgtcgccg
      721 ggctgctgct ggagcaggag gtgaccgccc tctccaacct gctcgccaag ccacccaagc
      781 cgttccacac catcatcggc ggggccaaga tcagcggaaa gctggaggtg ctcgaagtgc
      841 tgctggctcg gtgccaggcg gtcctggtgg gcggtggcat ggccaacacc ttcctggccg
      901 ccaagggtta cgccatgggc aagagcctgg tcgagaccga gcagctgggc aacgccgaac
      961 ggatcatgtc ggaggcgcgg cgcaagcggg tgcgcctgat gctcccgacc gacctggtca
     1021 tcgcagccca gatccacccg cgctcgcagc gccaggtcgt cccgctcaac ggcgtgccga
     1081 aggactggac cgtggtcgac atcgggcctg agacggtgaa gacctacacg acccacctgg
     1141 ccaaggcgcg gacgatcttc tggaatggac cgatgggcat cttcgagatc gccccattcg
     1201 ctgagggcac tgacgcgatt gccaactacg tggcctcgcg aacggccgat ggggtgatca
     1261 ccgtggtggg cggcggcgat tcggtatcgg ccgtcgacaa gctcggcctg accaaccaga
     1321 tgagccacgt ctcgaccggc ggaggcgcat cgctcgagtt cgtcgaggga aagaagcttc
     1381 ccggggtgga ggccctgccc gaccgcgacg cgccggcgaa atgaccaccg gcatcgcggg
     1441 ggtccagggg cgcgaggtgc tcgactcccg cggcaacccg acgatcgagg tcgaggtgcg
     1501 gctcgatggc ggcgtccggg gccgcgccat cgtcccatcg ggagcctcga ccggcgtcca
     1561 cgaggcggtg gagctgcgcg acggcgactc cgcccgctac ggcggcaagg gcgtgctgcg
     1621 cgcggtcggc aacgtcaacg gcgagatcgc ccaggcggtg gctggtcggg acgcgctcga
     1681 ccagcccggc ctcgaccgcg ccctgatcga cctcgatggc tcacctaaca aggggcggct
     1741 gggcgccaat gccctgctcg gcgtgtcgct ggccacggct cacgcggcag ccgccgcaca
     1801 gggtcagccg ctgtatcggt accttggcga cgacgcccac gtcctgcccg tgccgctggt
     1861 gaacatcctg aacggcggca agcacgccca ggactcgacc gatttccagg aattcatgat
     1921 cgcgccgctc ggcgcgccga ccttcgccga ggcgctgcgt tgggcggcag agaccttcca
     1981 cgcgctcggc gagctcctcc accagcgcgg cttcgccacg accgtgggcg acgagggcgg
     2041 ctatgccccc agcctggcga cgaacgaaga cgccatcgcc gtggtactgg aggccatcca
     2101 acgggccggc taccgaccgg gcgagcagat cgcgatcgcc ctcgaccctg ccgcgaccga
     2161 ggtctacagc gaggggcgct acgtcctcac ccgcgagaag cgcaccctca cgagtgacga
     2221 cctgatcacc ttctgggccg actgggtcgg ccgctatccg atcgtcagcc tggaggacgg
     2281 cctggccgag gacgactggg ccggttggac agcgctgacg gagcggctcg gggatcgcat
     2341 ccaggtcatg ggcgacgatc tgctggtgac caacaccgag cgcctggcgc gtgccatccg
     2401 ggagaaggcc gcgaactcga tcctgatcaa gctcaaccag atcggcacgc tgaccgagac
     2461 ccgcgaggcg gtcgagatgg cgcaggccgc cggctggcgg gcgatcgtca gccaccgctc
     2521 cggcgagacc gaggacacca ccatcgccga cctggtggtc gccctgggaa cggggcagat
     2581 caagaccggc agcgtgagtc gcggcgagcg gatcgcgaag tacaaccgcc tgctgcggat
     2641 cgaggaggag ctcggcgagg ctgccaccta cgccggccgc ggagccttcg cggcgcttgc
     2701 cgtctgatgg acggcctgac cgatcacgcg cagcgcaatc gggccttctg gaacgagcta
     2761 gccgacacct accaggccga ccacggtccc gacctggctc gccacggcgc cgcctggggc
     2821 gtctggcaga tccccgagga cgagattcac gccctcggcg atgtggccgg caaggacgtg
     2881 ctggagctcg gctgcggggc ggcccaatgg tccatcgtgc ttgctcaacg aggggcccgg
     2941 ccggttgggc tcgacctttc ggaacgccag ctcgggcatg cccgcgagtt gatggcggca
     3001 gcggggctcg acttcccgct ggtccacgcc agcgccgagg cggtgccata ccccgatgcc
     3061 agcttcgacg tcatcttctg cgaccacggc gccatggtct tcgccgatcc ccgtcgcacc
     3121 gtcccggaga cagcgcgcct gctgcgcgac gacgggctgc tcgtcttcag catgcatacc
     3181 ccgatcgcgg acatctgctg gccgcccgac tcggagcagg tcaccgacca gctgcggctc
     3241 aactactacg agctcaacgt cgtggacgtc ggagagtcgg agcacgtcga gttccagctg
     3301 ccgtacggtg agtggatccg gctgttccgg gccaatgggt tcgagatcga ggacctgatc
     3361 gagctccgac cggagcccga cgcggtcagc acctaccgca ccgaagagga ccgcgaatgg
     3421 gcgcggaaat ggccgatgga gcacatctgg aaggttcgcc gtcgacgcga ggtcgcttcc
     3481 tagctgactg catctccagg gcggaccggt ccctcgctca ccacccgcgc gtaccagcgc
     3541 gcctcgttcg ggtgcgtctt gtggctgatg cgcgcgaaac ggccgtcgct gaaccactgg
     3601 gcctgcttgg tgcagggggc ggcattccag gtcatctcga tctccagccc gccttcgccg
     3661 atgacgagcc gatcgccccc ggcgagcgac tccatctcga tcccctcaag ggtcaggttc
     3721 tccccgaagg cgccgggaaa ggcgttgtgt ccctccgcga ccagtcgttg gatcgcttcc
     3781 atcgagtaca ggcagaccgc ctggtccacg ccgccgtggc ttggctcggg ctcgatgtgg
     3841 cggtcatcgg ccaggcccat cgcgcccacc cactgctcat ccaccggcag cttggggacg
     3901 cctccctggt tgacgttgac gctgaccacg cgaccggctt ggggctcatc catgcgtcgg
     3961 gtctagccct cggcgggagc ctcgggctcg gcgtcgccac cggccttggc acgcttgcgc
     4021 gcggcggcgg tctcgcggga cgcacggacg acggcaacct tcgccttgcc ggccgcggtg
     4081 cgcggccgat cggcagcctt ggcggcggca gccttggatg ccttcgaagc ggctaccttc
     4141 ttgcgctgcg cggtcttggt ggcggtcgac ttcttcttcg gcgccgacgg agcctcgctc
     4201 gcggccccgt ccagcaggtg agcggcgttg gccttggcca tcagccggct cttgcggcga
     4261 gcggcgttcc tggggtggat gatccccttc tcggcggcct tgtcgagcgc gctgagggcg
     4321 gcggtcacgg cagcggcgcc atcgccctcg gagcggccca acgcgatgtt gctggcctgc
     4381 acgacgaggg tcttggcatg gctccgcgtg cgctggttga cggtgcgacg gcggagcgac
     4441 tggcggacgc gcttcagcgc ctgcggcgtg cgtgcgccct tccaggttcg agcttttctg
     4501 gccacgagca ggaatcctca ccgatgcgga aatggggcaa tgaaatgcgc cgtccggagg
     4561 gcggcgccac gggttgggag tatagcaccg ggccgctcgc ggcccctcga cgaggaggct
     4621 gtcgccaatt tgcagccagg ttcgcgttag ccacgctgag agacaatgga gacgccctcg
     4681 cggatcaatg gtgagcgtgg atgggcaatc ggcatcggcc ctggaggctc atttcccgcc
     4741 taagagcaac cagattgtga tctggcgacg aaacgatcga tccggccgca taagagcggc
     4801 cagactgcga gatggcaact tcgtgcagtc tccgcgcgac catgctccac cgctagactc
     4861 gcgctcgtga ttcccccgga gcgcacgcgc aacttcagca tcgtcgcgca catcgaccac
     4921 ggcaagagca cgctggcgga tcggctcctg gaggcgaccc acaccatcga tccccgccag
     4981 atgacgagcc aggtgctcga ctcgatggac ctcgagcgcg agaaggggat cacgatcaag
     5041 gcccgggccg tgcgactcca gtacgacgcg aaggacgggc agacctacgc gctcaacctg
     5101 atcgacacgc ccggccacgt cgacttcacc tacgaggtga gccggtccct ccaggcctgc
     5161 gagggcgcga tcctggtggt ggatgccagc caggggatcg aggcccagac cctcgccaac
     5221 atgcacctgg cgctggagca gcacctggtc atcatcccgg tcatcaacaa gatcgacctc
     5281 gcctcggcag acccggagat ggtgatggcc gagctcgagg agaccctcgc catcccccgc
     5341 gaggaggtga tcctggcctc cgccaaggag gggaagggga tcgacgacat cctcgaggcg
     5401 atcgtgactc gcatcccgcc gccgagcggc gaggcatcgg ctcccctcca ggcgctcgtc
     5461 ttcgacagcc actacgaccc gtacaagggc gtggtcgcct acgtgcgcgt cgccaacggc
     5521 acgctgcacg accacgagcg gatccgcttc atggccaccg gcatcgagac cgacatcctg
     5581 gagctcggct acttccggcc gcagccggtc ccgacccgtc gcctgacggt cggcgaggtc
     5641 ggctacgtgg cctccggtct gaagagcatt gccgacgccc gcgttggcga caccctgacc
     5701 gatgccgacc gacccgccgc cgagcccctg cccggctaca agcaggcgct gccgctcgtc
     5761 ttcgccggca tctacccgat gcgcggcgac gagtatccgc tcctgcgcga tccgctcgag
     5821 aagctgcacc tcaacgacgc ctccttcgtc tacgagcccg agtcgagcgt ggcgctggga
     5881 ttcggctatc gctgcgggtt cctgggtctc ctccacatgg agatcgtgca ggagcgcctc
     5941 gagcgcgagt tcgggctcga gctgatcgca tcggcgccat cggtcgaata ccgcgtcaag
     6001 cgcttgaaca gggccgatga gctggtcgtc gacaaccccg ccaggtttcc gccggcaggc
     6061 gaggtggaag cgatcagcga gccgtgggtg tcggccacgg tcatcacccc gaccgactac
     6121 atcggcccga tcatggagct ggccaccacc cgccgtggtg acttcatcaa catggagtac
     6181 ctcgacccca agcgcgtcaa cctccatttc gagatgccgc tggccgagct gatcatcgac
     6241 ttctacgacc agctgaagag ccgctcatcg ggctatgcca gcctcgacta cgcgtacctg
     6301 ggctatcggc ccggcgagct ggtcaagctc gacatcatgg tcagcggcga gccggtcgac
     6361 gcgctctccc tgatcgtgca tcggtccaat gcctttcggg tcgggaagtc actggtcgag
     6421 aaattgaagg agctgatccc tcgccagatg ttcgaagtgc cggtccaggc ggccattggt
     6481 tccaatatca tcagccgcga gacgatccgg gcgatgcgga agaacgtgct ggccaagtgc
     6541 tacggcggcg acatcacccg caagcgcaag ctcctggaaa agcagaagga aggcaagcag
     6601 cggatgaagc ggatcggctc cgtcgagatc ccgcaggaag ccttcctggc tatcctccgc
     6661 atgaacgagg ggcaatagcg tgaacttcca ggactcgttg cgcctctcga tcgccctggg
     6721 gctgacgatc ttcctggtgc tgctgcgctt cgactcggac cgcatcatgc gctccgacta
     6781 cttccgctcc cgttccggct gggtcgggcc gctctcgtac tacggcctgg tggtcctctt
     6841 cgcgatcggg attgccatca tcctgccgcg cggtcgtgcg cagctcttcc tggcgcccgg
     6901 cgaccccggt tcgatgctgc cggtgatgct cctcttctct gccgtggcga tcctcaacgg
     6961 gctggtgctc gccttcgtcc gctaccgcgc catcctgccg ctgccgaacc agctgctcgt
     7021 gtcccggctg gctggggcgg cggccaacgc gtttgccgag gagctccagt tccggtccat
     7081 cgtgctgggc atgctcctct tcgcgggttt gcccggaggc gtggggaccg ccctggtcgt
     7141 gcaggcgctg gtctacggcc tcgcgcatcg gcggctgtgg aaggagcgtg actggtactt
     7201 cctggcaggc tcggtgatcc tgggctatgc cgccgggctc gcgacggtga ccaccgggtc
     7261 ggtggtgccg gccatggtcg ggcacttcgc ggtgaccgcc tcgctcttcg cgttcgcggg
     7321 gggacggctg cgccagcggc cgatctagcc acgcggttcg tcgctgatta ccacgtgggg
     7381 tggtgcatcg gcaccggggg ctgccgtcct cggtccacga acgaccaaaa tgcgtccccg
     7441 taccactcat cgtggtgagc cgacgccaaa tgcagacacc ccataggcca tcggtgctgt
     7501 tgcaccacgt aggctggtaa acgggccgat catccccgct gacgtgggca tatcacgcgc
     7561 ggcgccagat agtcccgatg gtccatacgt gagtccccgc cagctccatg cagagagcgg
     7621 cagcacccac gtcgcgcgcc acccggcgcc ttacccagaa gggtgtgtcg atcctgggcc
     7681 taccggagcg tcgtacaggt agaaggcgat ccacagcgcc gatagcaatc cgagaaggag
     7741 atctcactca tggcagcagc caaggtcatt cacgtcgaaa tcacgggcaa ggatgtgccc
     7801 gcccttcaga agttctactc cgacatcttc gactggaagc tcgaaaccga caatccagag
     7861 ggctacggca tgaaccggga cgagtccggg ttcaccgccg gcatcggcgg cacccaggac
     7921 gggggagcgg gccaggtgac cttctatgtc cactccgacg acccggccgg cgtcctgcgc
     7981 cgcgtcgagg agcgcggcgg caaggtgatc atgccgctga ccgaagtggc caacgacacc
     8041 accatcgccc tcttcgccga tccggagggt cacgtcgtcg ggatcatgta gtcccgccgt
     8101 cgcacaccgc gaacgccgcc ccgagcccgc tccatgtagg ctcggggcgt gtcgttgccc
     8161 acgcaccacc tgtacgttca cgtccccttc tgccggttgg tgtgcgccta ctgcgacttc
     8221 gtgacagtcg gcgggcgaag cgccgagatc gcgcgctacc cggatctgct cctcgccgag
     8281 ctggcgctgc ggccggcatc gggccagctg gcgaccatct atttcggggg cggaacccca
     8341 tcgctgctcc cggccgatgg cgtagctctg gtggttggtg cggccattga ccgatggggg
     8401 accgccccaa ccgaggtgac cctggaggcc aacccaagct cccgcgagac gcccgattgg
     8461 tcggcgctgg gggcggccgg cgtcaatcgg atctcgcttg gcatccagtc gctccgcgac
     8521 cccgagctgc tctcgctggc ccgtggccag accgtggcgg aggggcttgc cgcatacgct
     8581 gcggtccgga aggcaggatt cgagaatgtc tcgatcgacc tcatctacgg cattcccgga
     8641 cagtccctgg ccgattggcg tcacgggttg cgcctcgccc tggcgctggc acctgagcac
     8701 ctctcgctgt acgcgctgca gctcgccatg gctcccgacg agtgggccgc gccgccgcgc
     8761 ccgggcgccc tgcgctggcg gcagcggatg gtggcgcgcc aggacgaccg cctggccgcg
     8821 gcccaatacc aggtcgccga agaggtgctc gaggtcgcgg gattccgcca ctacgagctc
     8881 tcctcatggg cgcgcccagg cttcgagtcg cgccacaact cggcgtattg ggagcggcgg
     8941 ccgtacaccg gaatcggcgc cggcgctcat tcattcgatg ggcgcacgcg ctcctggaac
     9001 gagcgagacc tcgaccgcta caccgctcgc accagcgctg gagaacgagc tgtggccggc
     9061 agcgaggagc tggatgagcc aaccagggcc ttcgaggcgg tcgcgctggg tctgcggcgc
     9121 gtcgacgggt tctcgcgttc gacgtttgcc gccgaattcg gcgccgatcc gctggttcgc
     9181 ttcgccagcg cagtggctga ggggaccgcg gccgaactgc tcgaggtcac cgacgacgcg
     9241 atccgcctta ccgcccgtgg acgcctgttc gccaacgagg cgctggtagc cttcgcgcca
     9301 tagccgcagg gatgctcagc atgtgagcat gtcaacaggg attcgtcccc aacgggccgg
     9361 aattggtccg attcccggct gttcggcgca gatactgagc atccggccgg aattcgagga
     9421 catcgtggtc cgtgtgctca gaggccgagc atctcgcccg gcggtgctcc gcgcgggcgg
     9481 gcgcgctgag ggcggctcgc gtcgcgacct ggcccgcgtt gacaggggtc cggcgaggtt
     9541 ggtacgatgc cagccgaacc attggcactc tcgttgttag agtgctaacg gacccgcaaa
     9601 ggcaccacaa cggaggtcag tcttggtcga ggggatggcc cgcgacgagg agagagcccc
     9661 gtctcggtcg gtgcttgacc tgaccgagcg gcagcgcatc gtgctgcgcg ccgtcgtcga
     9721 ggactacgtc ctgaatgcga taccggtcgg ttcacaggca ctcgtcgagc gctacgggct
     9781 gcccgtcagc tccgccaccg tgcgcagcgc catggccgag ctcgaggcgc tgggactcct
     9841 gagccacccg cacaccagcg cggggcgtgt cccgtctgac ctgggatatc ggacctacgt
     9901 ggagtccctc atgcgcgaat cggccctgga tcgcgccgat cagctgatga tccgtcacca
     9961 gttcagccag gttcagctga cgagcaacga gtggctgcgg cttgccgcgt cgatcctggc
    10021 caccagcacc cgctcggccg ccgtcgtgac gccggcccga gcgcgtcggg cgcgcttcgg
    10081 gcacctgcag ctggtcgagc tcgccgaccg cgcccgcctg gcggtcctcg tcttcggtga
    10141 cggcaacgtc gtccagcgcc ggctccaccg cacgagcctg gagaaggcga cccccggcga
    10201 gccggccggg caggcggagc tcgacacggc cgcggccacc ctcaacaggg agctggccgg
    10261 gctgtcggcg ccacaggtgc gacgaaagct ggggaagctg accccgctgg ccgcgcacgt
    10321 cgctgaggtg gtcgcccaga tgctcgagga gtccgacgcg gtcgcggtcg aggaggtctt
    10381 caccgacggg atcgtcaacg tgctcgagca gccggagttc gctggcggcg ccaagctgcg
    10441 cggggtgctc gaggtgctcc agcgctctga cttccttgag cagctggtgc cggtgctgag
    10501 ccgccgcggc ggcgtgacag tgatcatcgg ccacgagaac gtcaacgacg ccatgcacga
    10561 ggtgagcctc gtcttcgcgc catacgggac cgaggactcg gcgctcggcc tgctgggcgt
    10621 gctcggaccg acccgaatgc catatccccg cgccatcccg acggtgcgct atctctccac
    10681 cctcatgaac gagctgatca caaatcaccc gacggagaac catgactgag cgacccacca
    10741 acggcgcccc cgaagacacg cgccacgcgc gcggtccgtc gcgcgccgcg ctgatcgaga
    10801 agctggagga catgcagcga gtcctcgagg agacgcgtga gcagtccgac gagcacctgc
    10861 gcggcctgca gcgcaccgcc gccgacttcg ccaactaccg gcgccggatg gacgaggagc
    10921 gcgaagggct gtcgcagttc agcaacgcgt tgctgatcgg caagctgctg gccgtcctcg
    10981 acgacttcga ccgcgcgctc gacacggtgc cgcccgggac gaacgagggc tgggtcgacg
    11041 gcgtgcgcct cgtcgagcga aagctgcgtg gcctgctgga ggccgaaggc gtccgggaga
    11101 tcgacgcgct cggccagccc ttcgatccga acctgcacga agcggtcgtc tacgaggaga
    11161 ccgcagacca tcccgacaac caggtgatcg gtgagctaca gcgcggatac cggctcggtg
    11221 accgggtcct gcgcccatcg ctcgtccgcg tcgcgaacaa cccaaaggag cgctgaacat
    11281 atgggaaaga tcatcggcat cgaccttggc accaccaact cggtggtcgc cgtcatggag
    11341 ggcggcgagc cccaggtgat caccaacgcc gaaggtgggc gcataacgcc atcggtcgtg
    11401 gccttcgcca agagcggcga gcggctggtc ggccaggtcg ccaagcgcca ggcgatcacg
    11461 aaccccgaga acacgatctt cagcatcaag cgtttcatgg ggcgccgctt cgaggatccc
    11521 gaggtcgctc attccaagga cctggtgccg taccaggtgg agaaggatcc caaatccgat
    11581 ggcgtggtcg tgacgctggc caatggcaag accttcagcc cccctgagat cagcgccatg
    11641 atcctgcaga agctgaagtc cgatgccgag gcctacctgg gtgagaaggt gaccgaggcg
    11701 gtcatcaccg tgccggccta cttcgacgac acccagcgcc aggccaccaa ggatgccggg
    11761 cgcatcgcgg ggctggacgt caagcgcatc gtcaacgagc cgaccgcatc ggccctggcg
    11821 tacgggctgg acaagaccaa ggaagagaag attgccgtct acgacctggg cggcggcacc
    11881 ttcgacatct ccatcctgga gctgtccgag ggcgtcttcg aggtgcgcag caccaacggc
    11941 gacacgcacc tgggcggcga tgacttcgac cagcgggtga tcgactggct gtcggaggag
    12001 ttcaagaagg accagggggt cgatctgtcg aaggaccgca tcgcgctcca gcgcctgaag
    12061 gaggctgccg agaaggcgaa gatcgagctc tcgagcacga ccaccaccga gatcaatctg
    12121 cccttcatca gcgccgatgc ctcggggccg aagcacctgg tcatcagcct gacgcgcgca
    12181 aagctcgagg acctggttgc cgacctggtc agcaagacgt cggggccggt caagcaggcg
    12241 ctcgccgacg cgtcgctcaa gccgaccgac atcgacgagg tgatcctggt cggtggcatg
    12301 acccgcatgc cggccgtgat cgaggcggtc aaggagatgt tcgccggcaa ggagccgcac
    12361 aagggggtca accccgacga ggtggttgcc gtcggcgccg ccatccaggc cggtgtcctc
    12421 ggcggcgagg tcaaggacgt cctgctgctg gacgtgaccc cgctctcgct ggggatcgag
    12481 accctgggcg gcgtggcgac caagatgatc aaccgcaaca ccaccatccc gacgtcgcac
    12541 acccagatct tctcgaccgc atccgactcg cagccatcgg tggacatcgt ggtcctgcag
    12601 ggcgagcgcg agatggcctc ggacaacaag aagctggcca ccttccggct ggatggcatc
    12661 ccgccggctc cccggggcgt cccgcagatc gaggtcacct tcgacatcga cgccaacggc
    12721 atcctgaacg tggcagccaa ggacaaggcc accaacaagg agcagaaggt cactatcacg
    12781 gcctcctcca tgctcgccaa ggaggacgtc gacaagatgg tccgcgatgc tgccgagcat
    12841 gcggaggagg accgcaagcg ccgcgccgag atcgagtcgc gcaacgaggc ggacgctctc
    12901 acctaccagg cggagcgcct gatcaaggac atgggcgaca agctgtcgga tgacgaccgc
    12961 aaggagatca acgaccggat cgaggcggtc cgcgaagccc tcaaggggac cgatgcgggc
    13021 gtcgtcgact ccacgaagca gcagcttgcc gaggtcctgc agaagatcgg gacgcgcgcc
    13081 tacgaggcgg ctggaacccc cgagcccggt ggcgaggatg gctccggcgg cgagggcggc
    13141 gagggcggcg aagggggaga aggcggcgga ccgaccgacg gagccgcggc cgaagaggaa
    13201 ggcgagacga tcgaggggga gtacaaggaa gtgtgaatgg agcgcagggg ccaatccttg
    13261 gctggctatc gcaacagcct gcggaccggc ccccgccccc ctcaccgctc cttctctcta
    13321 agggtcctcg cgtccggctc cttgccgcgg gcaggagcgg ccatgtaagg cccgccggcc
    13381 cctttcgcac gtcgggaggt gtcacccctt ccccagatca caaccggatt gctcttacga
    13441 ggtcgcaacc cctttttcgt cgccggatct caacctggct gctcttaggt agtttcgagg
    13501 ccgttcgcac cccgattgtc cgaccggttc acgctcttcg ctggttgctg gacccgcaca
    13561 cgctgtcttc cagcacgtct aaggccaact ccactgcgat ctggcagttt ggtgagcagc
    13621 agcaggccgc tcggagtgcc ggacggctgc ctaatccagg cgagggcacc cctaccgcgg
    13681 agtccaaagc gcgcgaacag ccgtgagtgg tcagccaggc gccggcggtt ggtggtgcca
    13741 tcggcaacga ggaggaatgg aaccgcgcct gccgctcgcc agccgagcga ctgcgccacg
    13801 gtgggtgcga cccgctcttt cgtattgagc gttccaagca gatcctggat ctccgcgatg
    13861 accgtcttga cctcgacaac gacaagcgac cgcgtcgccg gatgaagcgc cagcagatca
    13921 atgcggccgc gatcgccgta gtggttgaag ctcgcctcgg gagcgaccgt ccaggcgaag
    13981 gcctccagtt gccgggtcag ccactcggcc agctgggcgt ggccggcgtc gcgcagccgc
    14041 gccagctccg cgcctcgata gccagcgtcc cagcccaaac ggaggtcaag cgccgaaccg
    14101 atgcgccgga gggaccggac agacagcgtc tccgcgtgtc cgcgctcgag cacaccgacc
    14161 agcgatgcgc tcacatcggc tttccgtgcc agatcgcttt ggcgccagcc ccggcgatgt
    14221 cggagcgcac gcacgatccc gcccaactct ccatcgttca tggctagatg atctggcccg
    14281 caacgtactg cggtgttatc aggccgttag gcagtcactc gatcggctcg actagcacag
    14341 cagccaggcg ccgaccagga gcgctccgta gaacaacggc agcaagactc accagcctgg
    14401 cggaggccat tttggtcgtg cgtagaatcg gccatgccag gcgggagcac cgagacgagg
    14461 aagcggagag cgcgaggacc gaccatgatc aagatctgga gcgagttgcg ggtcgcgagg
    14521 ttgaaggagc aggtcgcgga cctcgcgacg ctggtgtggg tcgtgttctg ggggaacctc
    14581 gtctggcagc tgttccagct cctgaccggc ttcgcgcagg ccggacgcac gatccactcg
    14641 ggcggcaagg ccatgatcca gagcggagtc gacatcgg
//
LOCUS       DATCQR010000196         5288 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_42342, whole genome shotgun sequence.
ACCESSION   DATCQR010000196 DATCQR010000000
VERSION     DATCQR010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5288
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42342"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            75..1349
                     /locus_tag="VIN32_06585"
     CDS             75..1349
                     /locus_tag="VIN32_06585"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012566.4"
                     /note="Catalyzes the transfer of electrons from NADH to
                     quinone; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HEY8922281.1"
                     /translation="MTSEVKIPDPLDLDRDEEARLRRVAGLPAAKTPAGEYAGFESVG
                     LPERPPQTEREREVYTLEEGEMLVNMGPQHPSTHGVMRLVVKVRGEVVTDVDPVLGYL
                     HRGIEKLCEEATYTTVLTYVDPMDYLSTMHNEHAPAIAFEKLFGVTAPKRAEYIRALV
                     VELNRVFSHSLMMGFLALDMGGITPILYGFINRDEIVDMLSAISGQRMLFNFFRVGGV
                     NWDTNDEFLSRLGTWRGHVLKNVEMSERVLTDNEVFRSRTIGMGTLSGKDAIALGVSG
                     PNLRASGVPFDVRKAHPYSIYPELEFEVITQSGGDSNARYLQRIAEIKQSIHLIDQIV
                     DKMPHGAVQAQVPGIIRPKPGRAYAAIESPRGLYSVYAISDGGPNPYRFRMRDPSFIS
                     LQAIPKLMPGRLIADTMAVMASFDPVMGGVDK"
     gene            1346..2356
                     /gene="nuoH"
                     /locus_tag="VIN32_06590"
     CDS             1346..2356
                     /gene="nuoH"
                     /locus_tag="VIN32_06590"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HEY8922282.1"
                     /translation="MNIDLLIGVLRFLLATTLILLLTIPTAFVVIQMELKIIAGAQLR
                     YGPNRVGPWGMFQSTIHGFKVLAKEDQIPDQADRATFTLAPWMVYMAAAMSMLVIPFA
                     PGAIAADFNIGIVYFFAVLSLSVVGLLIAGWASYNKYSLLGGLRSAAQLVSYEIPLTL
                     SVVGAVVLAGTLNFRELIEWQRAHGWMLLWQPVGLVIFYIASLAEINRTPFDMVEADS
                     ELVAGPFTEYSGMRFGFFFFAEYVALFIMSGIMISLFFGGWLAPWPFPDQLDGFVGTL
                     YGVLWFFIKTYLFVALAVLLRATLVRVRIDQLMGMAWKVLLPLAMVNLLVTAAAVLVF
                     KL"
     gene            2353..3195
                     /locus_tag="VIN32_06595"
     CDS             2353..3195
                     /locus_tag="VIN32_06595"
                     /inference="COORDINATES: protein motif:HMM:NF013426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HEY8922283.1"
                     /translation="MTIPGLGILKGLRQTARNYWAPKVTNQYPESRPTMPDRWRGRLD
                     LIYDPFGEHKCEVCFQCAQVCPVEAIDMSGFDSTGQRIRYGLPEIYDERKDPNAYRRA
                     GLPAAPMRNAARWDEEIDTPWLHGLIDGFGGKPEALVAIFSAVEEHYGYLPEQALREA
                     SERMSIHWAQIFGAAGLGGFRLLPVDGHLVTVCTCAACRFAGGEALLNALRTDLGISP
                     GETTADGAITLATSDEVAAGALSPALRIDTMVYGPLTVEQARHVVEERRKAPSRGRAA
                     QSVS"
     gene            3192..4547
                     /locus_tag="VIN32_06600"
     CDS             3192..4547
                     /locus_tag="VIN32_06600"
                     /inference="COORDINATES: protein motif:HMM:NF013662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HEY8922284.1"
                     /translation="MTDASPGLSPQIRILPGGDQRLLAEIGVVDPTSLDSYRAAGGYA
                     GLKRAVEKLGPEGTIDEIAAAGLLGRGGGGYPTADKWHAARAATGDSKVVVANLIGAD
                     PTALGDRALAEGNPHLLLEGLLIAAFATGASDAIIAVRRDWTVAIERLRAAIAEAETA
                     HLAGYLVMGTDFSCVVRVWEGAVALVAGEETALLAALAGDRGMPVIRPPYPTERGLGG
                     APTTVQNGETLAHAAWIASHGAKKFRAIGSKEAPGTKLVSIYGRVAQPGLVEVALGTP
                     LSEIVALAGGGTGTTKAVFVGGPGGGALRADQLDTPYDFVPLHAAGAGIGLGQLLVAD
                     SDTCMVDSARFFLDYSAREACGKAVPCRIGTKRLVETLDRILAASPRPNDLLLLRELS
                     RKMTDTALCHLEARAPGPMLTTLDRFPDEYRAHAERGECLAGSCSTSRLPPLLTPLAG
                     L"
     gene            4560..>5288
                     /locus_tag="VIN32_06605"
     CDS             4560..>5288
                     /locus_tag="VIN32_06605"
                     /inference="COORDINATES: protein motif:HMM:NF026042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEY8922285.1"
                     /translation="MSFSTNGNGNGKGEPLRPEDYVPIWPMVPGTRKISVTINGQEVQ
                     AEEGQTILQATRVLGIDVPTLCYEPKLAPYGACRICVVEVEGQENTPISCGTQIEEGM
                     VVTTHSDRLVENRRMVLELIFSDHDAYCLPPCQFKCPTRVDIPGYLKQNTLGNWEEAT
                     RILKRSLPFPAILGRVCPAPCETHCRREEVDTAIAIRDSHRYCADRVLEVDAPAPTPW
                     PKEPDSGRRVAVIGSGPAGMSAAYY"
BASE COUNT          828 a         1866 c         1778 g          816 t
ORIGIN      
        1 gacctgagcc gcctctccga cgagctggcg cccggcgtcg tcgcccatgc cgaggccgag
       61 gcagaggcca agccatgacc agcgaggtga agatccccga cccgctcgac ctggaccggg
      121 acgaggaggc gcggcttcgt cgggtggccg ggcttcccgc ggcgaagacg cccgccggcg
      181 agtacgccgg cttcgaatcg gtgggcctgc cagagcggcc gccgcagacc gagcgcgagc
      241 gcgaggtcta caccctcgag gagggggaga tgctggtcaa catgggcccg cagcacccct
      301 caacccacgg cgtcatgcgc ctggtggtca aggtccgcgg cgaggtggtg accgatgtcg
      361 atccggtcct cggctacctt catcgcggca ttgagaagct gtgcgaggag gcgacctaca
      421 ccaccgtcct gacctatgtc gacccgatgg actatctctc gacgatgcac aacgagcacg
      481 ctcccgcgat cgccttcgag aagctgttcg gggtcaccgc tccgaagcgg gccgaataca
      541 tccgggcgct ggtggtggaa ctgaaccgtg tcttcagcca cagcctgatg atgggcttcc
      601 tggccctgga catgggtggc atcaccccga tcctgtacgg cttcatcaac cgcgacgaga
      661 tcgtcgacat gctctcggcg atcagcggcc agcgcatgct cttcaacttc ttccgggttg
      721 gtggcgtcaa ctgggacacg aacgacgagt tcctctcgcg cctcggcacc tggcgcggcc
      781 atgtcctgaa gaacgtcgag atgagcgagc gcgtgctcac cgacaacgag gtcttccgct
      841 cgcggacgat cgggatggga acgctctcgg ggaaggacgc gatcgccctc ggcgtttcgg
      901 ggccgaacct gcgcgcttct ggggtgccgt tcgacgtccg caaggcgcat ccctactcga
      961 tctatcccga gctcgagttc gaggtgatca cccagtccgg cggcgactcg aacgcccgct
     1021 acctgcagcg catcgccgag atcaagcaga gcatccacct gatcgaccag atcgtggaca
     1081 agatgccgca cggcgcggtg caggcgcagg tgccggggat cattcgcccc aagccgggcc
     1141 gggcctacgc cgcgatcgag tcgccgcgcg gcctgtacag cgtctacgcc atcagcgacg
     1201 gcgggccgaa cccgtatcgg ttccggatgc gcgacccgag cttcatcagc ctgcaggcga
     1261 tccccaagct gatgcccgga cggcttatcg ccgacacgat ggcggtgatg gccagcttcg
     1321 acccggtgat gggcggcgtc gacaagtgaa catcgacctg ctgatcgggg tcctgcgctt
     1381 cctgctggcc accaccctga tcctgctgct gaccatcccg accgccttcg tcgtgatcca
     1441 gatggagctc aagatcatcg ccggcgccca gctccgctac ggcccgaacc gggtcggccc
     1501 gtgggggatg ttccagagca cgatccacgg cttcaaggtg ctcgccaagg aggaccagat
     1561 ccccgaccag gccgatcggg caaccttcac cctggccccg tggatggtct acatggcggc
     1621 cgccatgagc atgctggtga tcccgttcgc gcccggcgcc atcgccgccg acttcaacat
     1681 cgggatcgtc tacttcttcg cggtgcttag cttatcggtg gttgggctgc tgatcgccgg
     1741 ctgggcgagc tacaacaagt actcgctgct gggaggcctg cgcagcgcgg cccaactggt
     1801 gagctacgag atcccgctca cgctgtcggt ggtgggtgcg gtggtcctgg caggcacgct
     1861 caacttccgc gagctgatcg agtggcagcg cgcccacggc tggatgctgc tctggcagcc
     1921 ggtcggactg gtgatcttct acatcgcctc gctggccgag atcaaccgca ccccgttcga
     1981 catggtcgag gccgacagcg agctggtggc agggcccttc accgagtact ccggcatgcg
     2041 tttcggcttc ttcttcttcg ccgagtacgt ggcgctcttc atcatgagcg ggatcatgat
     2101 ctcgctcttc ttcggcggct ggctggcgcc gtggcccttc cccgaccagc tcgacggttt
     2161 cgtcggcacc ctgtacggcg tcctctggtt cttcatcaag acctacctct tcgtggcgct
     2221 ggcggtgctg ctgcgcgcaa ccctggtgcg ggtgcggatc gaccagctga tggggatggc
     2281 ctggaaggtg ctgctgccgc tggccatggt caacctgctc gtcaccgccg cggcagtgct
     2341 ggtcttcaag ctgtgacaat cccgggcctc ggcatcctca aggggctgcg ccagaccgcg
     2401 cgcaactact gggcgcccaa ggtcaccaac cagtacccgg agtcccggcc gaccatgccg
     2461 gaccgatggc gtggccgact cgacctgatc tacgacccgt tcggcgagca caagtgcgag
     2521 gtctgcttcc agtgcgcgca ggtttgcccg gtggaggcga tcgacatgag cggcttcgat
     2581 tcgaccgggc agcgcatccg ctacgggctg ccggagatct acgacgagcg caaggatccc
     2641 aacgcctacc gccgagccgg ccttccggcc gcgccgatgc gaaacgccgc gcgctgggac
     2701 gaggagatcg acacgccctg gctgcatggc ttgatcgacg gcttcggcgg caagcccgag
     2761 gcgctggtcg cgatcttctc ggccgtcgag gagcactacg gctacctccc cgagcaggcg
     2821 ctgcgcgagg cgagcgagcg gatgtcgatc cactgggccc agatcttcgg ggcggcgggc
     2881 ctgggcggct tccgcctgct ccccgttgac gggcacctgg tcaccgtctg cacctgcgct
     2941 gcctgccgct tcgccggcgg cgaggccctc ctcaacgcac tgcgaacgga tctcgggatc
     3001 tctccggggg agacgacggc cgacggtgcc atcaccctcg ccaccagcga cgaggtcgcg
     3061 gccggggccc tctcgccggc cctccgcatc gacaccatgg tctacggacc cctgaccgtg
     3121 gagcaagccc gccacgtcgt ggaagagcga cgcaaggcac cctcacgcgg gcgcgccgct
     3181 cagtccgtct catgaccgac gcgagccccg gcctctcccc gcagatccgg atcctgcccg
     3241 gcggcgacca gcggctgctg gcggagatcg gggtggtgga ccccaccagc ctcgacagct
     3301 accgcgcagc gggtggctat gccgggctga agcgcgccgt cgagaagctg ggtcccgagg
     3361 gcacgatcga cgagatcgcc gccgcgggcc tgctcggccg gggtggcggc ggctacccga
     3421 ccgccgacaa gtggcatgcc gcgcgggcag cgacgggtga cagcaaggtc gtcgtcgcca
     3481 acctgatcgg cgccgacccg actgcgctcg gcgaccgggc gctggccgag ggcaatccgc
     3541 atctcctgct ggaggggctg ctgatcgccg ccttcgccac cggcgcctcg gacgcgatca
     3601 tcgcggtccg gcgtgactgg acggtcgcca tcgagcggct gcgcgcagcg atcgcggagg
     3661 ccgaaacggc gcacctggcc ggctacctgg tcatgggcac cgacttctcc tgcgtggtgc
     3721 gcgtctggga gggcgcggtc gcgctggtcg cgggcgagga gacggccctg ctggccgccc
     3781 tggccgggga tcggggcatg ccggtcatcc ggcccccgta ccccaccgaa cgaggcctgg
     3841 gcggcgcccc gaccacggtg cagaacggcg aaacgctggc gcacgcggcg tggatcgcga
     3901 gccacggtgc caagaagttc cgcgccatcg gctccaagga ggcaccgggc accaagctgg
     3961 tcagcatcta cggccgcgtg gcgcagccag ggctggtcga ggtggcgctc ggcacaccgc
     4021 tctccgagat cgtggcgctg gctggcggcg ggaccggaac gaccaaggcc gtcttcgttg
     4081 gcggtccagg cggcggcgcc ctgagagcgg accagctaga cacgccatat gacttcgtcc
     4141 cgctgcacgc cgccggtgcc ggcatcggcc ttgggcagct cctggtggca gacagcgaca
     4201 cctgcatggt cgactcggcc cgcttcttcc tcgactactc cgcccgcgag gcgtgcggca
     4261 aggcggtccc gtgccggatc ggcaccaagc ggctggtcga gacgctagac cgcatcctgg
     4321 cggcgagccc acggcccaac gacctgctgc tcctgcgcga gctgtcccgc aagatgaccg
     4381 acacggccct ctgccacctc gaggcgcgcg cgcccggacc gatgctcacc acgctcgacc
     4441 gcttccccga cgagtaccgc gcccacgccg agcgcggcga gtgcctggcc ggctcgtgca
     4501 gcacgtcgcg gctcccgccc ctgcttacgc cactggcggg cctctaagat gcaccggcca
     4561 tgagcttctc gaccaacggc aacgggaacg gcaagggcga gccgctgcgc ccggaggact
     4621 acgtccccat ctggccgatg gtgccgggga cccgcaagat cagtgtcacg atcaacggcc
     4681 aggaggtcca ggccgaagag gggcagacca tcctgcaggc cacgcgggtg ctcgggatcg
     4741 acgtcccgac gctgtgctac gagccgaagc tggcgcccta cggggcctgc cgcatctgcg
     4801 tggtcgaggt cgagggccag gagaacaccc cgatcagctg cggaacgcag attgaggaag
     4861 ggatggtcgt caccacccac tccgatcggc tggtcgagaa ccgccgcatg gtgctggagc
     4921 tgatcttcag cgaccacgac gcctactgcc tgccgccctg ccagttcaag tgcccgacca
     4981 gggtcgatat ccccggctac ctgaagcaga acacgctggg caactgggaa gaggcgacgc
     5041 gcatcctgaa gcgctcgctg cccttcccgg ccatcctggg ccgcgtctgc cccgcaccat
     5101 gcgagaccca ctgccggcgc gaggaggtgg acaccgccat cgccatccgc gactcgcacc
     5161 gctactgcgc ggaccgggtc ctcgaggtgg atgcgccggc gccaaccccg tggcccaagg
     5221 agcctgactc gggccgtcgc gtggcggtca tcgggtcggg cccggccggc atgtcggccg
     5281 cctattac
//
LOCUS       DATCQR010000197         7123 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_227173, whole genome shotgun sequence.
ACCESSION   DATCQR010000197 DATCQR010000000
VERSION     DATCQR010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 7123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7123
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227173"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            85..567
                     /locus_tag="VIN32_06610"
     CDS             85..567
                     /locus_tag="VIN32_06610"
                     /inference="COORDINATES: protein motif:HMM:NF039927.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5946 family protein"
                     /protein_id="HEY8922286.1"
                     /translation="MPGLSGSGSRRRGDAASTLHRVGAGLLVVVQRGAGSEYGDLRYT
                     PAHQLTVDAYAVQHPGLPERRTAQSIAIHLVGLCLSLERGRGPMELPRLRQRLAPPRR
                     IFPWLPPPGSLGGVTVADVHATQTPEAHRETVGRWARSAWEAWSPHHSTVRAWADELG
                     "
     gene            complement(570..1640)
                     /locus_tag="VIN32_06615"
     CDS             complement(570..1640)
                     /locus_tag="VIN32_06615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8922287.1"
                     /translation="MVAQLGLRRPVRREGAEQYLFLTLVSFAATVMATRVYLELTGYP
                     RIGGGELHIAHALFGGFFLFAAALLPIVLAGRRVYRTTAVLGGIGIGLFIDEVGKFIT
                     TQNDYFYPAAAPLIYATFLLAVLVYLRVRRRTQADPRSLLLTSLQLMEEAVDNDLQDD
                     ERDALMSRLGSAALEAPLQEQRRLAAALLAFVQSQDLDIAPDEVSRLQPLFDWWAAHR
                     EDWIGGRGARAVLMAALFISGVRAVIALGGAVSQIGGVDPETSTRLFSLDMLHLVVEG
                     VAGALLLTGSLLMALRGRDRLGSLVAEFGLLVSLCLADLASFYLRQFATVEIALLHGL
                     LLSGVVAYRRQLHAEVPALNPP"
     gene            1731..3404
                     /locus_tag="VIN32_06620"
     CDS             1731..3404
                     /locus_tag="VIN32_06620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HEY8922288.1"
                     /translation="MTIATPAAAPEWDRAPLELPQRTRIEIFIVVLLSIFLAALDQTI
                     VGTALPVIVTDLKGNDVYIWAFTAYLLTATVSGPLYGKLSDLFGRRIIIMIGVSVFLI
                     GSVLCAISGEMWQFIIFRGIQGLGAGALFPVSLAIIADIFAPSERGKYQGFFGAMFGI
                     ASLIGPAIGGILTDTVGWHWIFLVNLPLGLVVLVVIWRTLPTRIESGVERTIDYLGAA
                     VFVAALVPILIGLTNKQFGEWSDPEVGGLIALGLGLAAIFVWVESRAREPILPLGLFR
                     IRAFTTSVFAMFFAMMGFFAAIVFMPRWYQVVQGASATESGYQILPLLGGLIVSAIVS
                     GQVVARTGRYKPIIFVALLFLALGLFLLTNLRTETDAPLVWLWMALTGIGIGPAFAVF
                     TLAVQNNVPPVELGTATSSLTLFQQVGGTVGLALTGTIFGSALLEEMPKQISAAGVPP
                     ELAGQFAQGGASALNNLSGVGDLGAKILAGLPPDAQAQIKPVIPLIVQGIHEAFSIAT
                     AATFVIGIGAALFAALLVLVGLPGGRIGEAAEANATAPVPGELSREVGA"
     gene            complement(3421..3996)
                     /locus_tag="VIN32_06625"
     CDS             complement(3421..3996)
                     /locus_tag="VIN32_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013772704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HEY8922289.1"
                     /translation="MQYRGRPLIHPKTIAAWREWLTRHHHDTDGVWLARWKKASGKTP
                     LDYAQIVEEALCFGWIDGVTNTLDDGRQAQLLTPRRRGSGWSPSNKERVQRLIADGRM
                     TESGRRVIEAAKADGSWSMQDAAEALIEPDELAGALDANPDARRHWDAFPRSPRRALI
                     WWVMSAKRPETRQRRVRTIVEEASQGRRANF"
     gene            complement(4023..4880)
                     /locus_tag="VIN32_06630"
     CDS             complement(4023..4880)
                     /locus_tag="VIN32_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016828037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HEY8922290.1"
                     /translation="MTFRPGTRLDPGQVRDMRGRGGALAVGGGLGGVVVLVLVMLLGG
                     SPQDAATLTGILDKTTVGNQTPNDLSQECQTAADANSRDDCRIIGYVNSVQAYWEKVV
                     DGYTLAPTTFYTDATNTGCGQATSAVGPFYCPEDDTVYIDLGFFDTLQTQFGAEGGPF
                     AEAYVIAHEYGHHVQDLLGTLQAGGGGTGAQSQSVRTELQADCFSGVWASHAVETGFL
                     NPLTPDQIGQALDAASAVGDDRIQEKTQGQVNPETWTHGSSAERQQWFKTGLDTGDPT
                     ACDTFKGNL"
     gene            4976..5392
                     /locus_tag="VIN32_06635"
     CDS             4976..5392
                     /locus_tag="VIN32_06635"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEY8922291.1"
                     /translation="MPEDAVELISSLSLFADLGGPQLEAISHIFEETTFASGQRILRQ
                     GITGSNFYVVVDGEASVRIDGEERANIGRGDFFGEMSLLLGSAPTADVTARTPLRCLV
                     LAGPELHGFLDKYPTVMYRMLVAMARRLSAANQHSG"
     gene            5397..6914
                     /locus_tag="VIN32_06640"
     CDS             5397..6914
                     /locus_tag="VIN32_06640"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEY8922292.1"
                     /translation="MARAPAKATAADRPFPPGAYPVVVVGSGPGAIQVSHELDRLGIG
                     HATISADPSPGGMFRSLPIYQRLLSWTKPYALGDPAGRAGEWYDWNSIVSDDPALRAI
                     MPGLMDGTSSFPSRAKMEENLATFVTRANLQIRYGCPWQSTSRADDGRFVLTTPDGDY
                     VCEVAIFAVGMAEPWKPPTPGLELVPHYVEAGPAESYAGKRVFIAGKQNSAFEIATAF
                     LPWARQLVLASPRPATLSVVEHSLAGVRARYVQPYEDAMLHNGVFLLDVKIERIERAD
                     DGFRVRLSRTDGGRELVCEADAAIATTGFAVPMRDLPALGVATFAQGKMPALTPLWES
                     ATVPGIFFAGTITQAAAGLKKHGIPANSGALHGYRYNARVLVRHLAATRFGIETPSPA
                     IEADAVSSFLLEQLTLAPELWNQRSYLVQVASVSDDGWRDDGIWPLADFVDASGPDAV
                     AVTLEPNPDSSIYPVAYVRISGAVEEHLLDPDAMNEYRGSPHREQMAAILAPLMR"
     gene            6943..>7123
                     /locus_tag="VIN32_06645"
     CDS             6943..>7123
                     /locus_tag="VIN32_06645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8922293.1"
                     /translation="MIRRLTAAADALRRVLANPDMRRAQLAWLLGYAGEWSWLVALWV
                     YAYQTSGVVAVGALGL"
BASE COUNT         1086 a         2437 c         2406 g         1194 t
ORIGIN      
        1 acgagacagt cgcaacgatc gactacgggg cccccgaggg atccctggac cccggcgacc
       61 tgaaccgatg agcgcctccg tcacatgcca gggctgtcgg gcagcggttc ccgacgtcga
      121 ggagatgcag catcgaccct acatcgggtc ggcgccgggc tgctggttgt tgtacagcga
      181 ggtgctggct cggaatatgg agacttgcgc tacacgccag cgcatcagct gaccgtggat
      241 gcgtacgccg ttcagcaccc gggcctgccc gagcggcgca cggcccagtc gatcgccatt
      301 cacctcgtgg ggctctgcct cagcctggaa cgtggacgcg ggcccatgga gttgccacgc
      361 ctgcgacagc ggctggcacc gccgaggcgc atctttcctt ggctgccccc gcccggctcg
      421 ctgggtgggg tcacggttgc cgacgtccat gccacgcaga cgccggaggc tcaccgagag
      481 accgtgggcc gctgggcgcg atcggcgtgg gaggcgtggt cgccacacca cagcacggtg
      541 cgtgcctggg cggatgagct gggctgaccc tatggcggat tgagggccgg gacctcggcg
      601 tgcaactgcc ggcgatacgc caccacgccg gagaggagca gcccgtgcag cagggcaatc
      661 tcgacggtcg cgaactgccg caggtagaag gatgcaaggt cggccaggca gagggagacc
      721 agcaggccga attcggccac gagggatccc aggcggtccc gcccacggag cgccatcagc
      781 agcgatccgg tgagcagcag tgcgcccgcc acgccctcga ccaccaggtg cagcatgtca
      841 aggctgaaga gccgggtgct cgtctcggga tccacgccgc cgatctgcga taccgcgcca
      901 ccaagggcga tcactgcacg gacgcctgag ataaagaggg cggccatcag gacggctcgc
      961 gccccccgac cgccgatcca gtcctcccgg tgcgcggccc accagtcgaa gagcggctgc
     1021 aggcggctga cctcgtccgg cgcaatgtcc aggtcctgcg actggacgaa ggcaaggagc
     1081 gcagccgcga gccggcgctg ctcctggagc ggtgcctcga gggccgcgga tccgagccga
     1141 gacatgagcg cgtcgcgctc gtcgtcctgc aggtcgttgt cgaccgcctc ctccatcagc
     1201 tgcagcgagg tgagcagcag gctgcgtgga tctgcctggg tccgtcgccg cactcggagg
     1261 tagaccagca cggccagcag gaaggtcgcg tagatcagcg gcgccgcggc ggggtaaaag
     1321 tagtcgttct gggtggtgat gaatttgccg acttcgtcga tgaagaggcc gataccgatc
     1381 ccgcccagga ccgcggtcgt gcgatagacc cgccggccgg cgagcacgat tggcaacagg
     1441 gcggctgcga acaggaagaa gccaccgaac agggcatggg cgatgtgcag ctctccgccg
     1501 ccgatccgcg ggtagccagt cagctccagg tagacgcggg ttgccatcac cgtggcagcg
     1561 aaggagacga gcgtcaggaa caggtactgc tcagctccct cgcgacggac ggggcgacgg
     1621 aggccgagct gcgcgaccat ggcggggccg tcgctggctg gagatccgcc gtcctgcctc
     1681 tagggcatca ttgggtcgcc aacccagagc ttttggagag accttcccga atgaccatcg
     1741 ccacgcccgc cgctgccccc gaatgggacc gagcccccct cgagctcccg cagcgaaccc
     1801 ggatcgagat tttcatcgtc gtgctgctga gcatcttcct ggccgccctg gaccagacga
     1861 tcgtcggcac cgccttgccc gtcatcgtca cggatctcaa gggcaacgac gtctacattt
     1921 gggccttcac ggcatacctg ctgaccgcga cagtcagcgg cccgctgtac ggaaagctgt
     1981 cggacctgtt cggacggcgg atcatcatca tgatcggcgt cagcgtcttc ctgatcggct
     2041 cggtcctgtg cgccatctcg ggcgagatgt ggcagttcat catcttccgg gggatccagg
     2101 ggctcggcgc cggcgcgctc ttccctgtct cgctggcgat catcgccgac atcttcgcgc
     2161 cgtccgagcg gggtaagtac cagggcttct tcggcgcgat gttcggcatc gcgtcgctca
     2221 tcggcccggc catcggcggg atcctgaccg acaccgtcgg ctggcactgg atcttcctgg
     2281 tgaacctgcc gctcgggctg gtcgtcctcg tcgtcatctg gcgcaccctc ccgaccagga
     2341 tcgagagcgg cgttgagcgg acgatcgact acctgggggc ggccgtcttc gtggcggccc
     2401 tggtgccgat cctgatcggc cttaccaaca agcagttcgg cgaatggtcc gatccggagg
     2461 tcggcggact gatagccctt gggcttggat tggcggcgat cttcgtctgg gtcgagtcac
     2521 gggccaggga gccgatcctg ccgctgggcc tgttccgcat ccgagctttc actaccagtg
     2581 tgttcgcgat gttcttcgcg atgatgggct tctttgccgc catcgtcttc atgccgcgct
     2641 ggtaccaggt ggtgcagggc gcctcggcaa ccgaatcggg ctaccagatc ctgcccctcc
     2701 tcggtggcct gatcgtctct gccatcgtct ccggccaggt ggtcgcccgt accggccgat
     2761 acaagccgat catcttcgtc gcgctgctct tcctggcgct cggcctcttc ctgctgacca
     2821 acctccgaac cgaaaccgac gccccactgg tctggctctg gatggctctg acagggatcg
     2881 gcatcggccc agcctttgcc gtcttcaccc tggcggtgca gaacaacgtg ccgccggtcg
     2941 agctcggcac cgcgaccagc agcctgacgc tcttccagca ggtgggcggg acggtggggc
     3001 tggcgctcac cggcacgatc ttcggatcgg ccttgctcga agagatgccg aagcagatct
     3061 ccgctgccgg tgtcccaccc gagctcgccg gccagtttgc gcagggtggc gcgtcagccc
     3121 tcaacaacct ctccggagtg ggggacctcg gcgccaagat cctcgccggc ctgccacccg
     3181 acgcccaggc gcagatcaag ccggtcatcc cgctgatcgt ccaggggatc cacgaggcat
     3241 tctcgatcgc caccgccgcg accttcgtga tcggcatcgg cgctgcgctg ttcgccgccc
     3301 tcctggtcct ggtaggcctc cccggtggcc ggatagggga ggctgccgag gccaacgcca
     3361 cggccccggt cccgggcgag ctgagccgcg aggtgggtgc ctaagaggct tggggccttt
     3421 tcagaagttc gcgcgccgcc cttgcgaagc ctcttcgacg atggtcctca cccggcgctg
     3481 acgcgtctcc gggcgcttgg ccgacatgac ccaccagatg agggcgcggc gtggtgacct
     3541 cgggaacgcg tcccagtggc gacgagcatc ggggttcgcg tccagcgcgc cagccagctc
     3601 atccggctcg atgagggcct cagcggcatc ctgcatcgac cacgagccgt cggccttggc
     3661 ggcctcgatg acgcgccggc cagactcggt catgcgtccg tcggcgatga ggcgctggac
     3721 gcgctccttg ttcgagggag accagccgct gccgcgacgg cgcggcgtca ggagctgcgc
     3781 ctggcgcccg tcgtcgagcg tgttggtgac gccgtcgatc caaccgaagc agagcgcctc
     3841 ctcgacgatc tgggcgtagt caagcggggt cttgccgctg gccttcttcc aacgcgccag
     3901 ccagacgccg tcagtgtcat ggtgatgcct ggtcagccac tcacgccacg ccgcgatcgt
     3961 cttcgggtgg atgagcgggc ggcctcgata ctgcatcgag cagcgatcct agcgcgccgc
     4021 tcctagaggt tgcccttgaa cgtgtcgcag gcggtgggat cgcccgtgtc gagaccggtc
     4081 ttgaaccatt gctggcgctc tgccgaagag ccatgggtcc aggtctcggg attcacctgg
     4141 ccctgggtct tctcctggat cctgtcgtca ccgacggccg acgcggcgtc gagggcctgg
     4201 ccaatctggt ccggcgtcag cgggttcagg aagcccgtct cgactgcgtg gctggcccac
     4261 acgcccgaga agcagtcggc ctgcagctcc gtccggaccg actggctctg cgctccggtg
     4321 ccgcccccgc cggcctgtag cgtccccagc aggtcctgga cgtggtggcc gtactcgtgg
     4381 gcgatgacat aggcctcggc gaagggcccg ccctccgcgc cgaactgcgt ctgcagcgtg
     4441 tcgaagaagc caaggtcgat gtagacggtg tcgtcctccg ggcagtagaa cggcccgacg
     4501 gcgctcgtcg cctggccgca gccggtgttg gtggcatcgg tgtagaaggt ggtgggcgcc
     4561 agcgtgtaac cgtcgaccac cttctcccag taggcctgga cgctgttgac gtacccgatg
     4621 atccggcaat cgtcgcggga gttggcgtcg gcagcggtct ggcattcctg gctgagatcg
     4681 ttgggcgtct ggttgccaac cgtcgtcttg tccaggatgc cggtaagggt ggcggcgtcc
     4741 tgcggcgagc cccccagcaa catcaccagc acgagcacga cgactccgcc caggccgcca
     4801 ccgacggcga gcgcgccgcc tcggcctcgc atgtcccgga cctggccggg atcgaggcgc
     4861 gtgcctggtc ggaaggtcat gcctgaacga taggaaagca acattgcagc ggcgcgtcgc
     4921 gaagcgctga cgccattaca ggcgccctca acacgcgccg actaccatgg cctccatgcc
     4981 cgaggacgcc gtcgaactga tctcgtccct gagcctcttc gccgacctcg gcggcccgca
     5041 gctcgaggcg atcagccaca tcttcgagga gacgaccttc gcgtcaggtc agcgcatcct
     5101 gcgccagggg atcaccggct cgaacttcta cgtggtcgtg gacggcgagg catcggtcag
     5161 gatcgatggg gaggagcgag ccaacatcgg gcgaggcgat ttcttcggtg agatgtcgct
     5221 cctcctcggc agcgccccga ccgccgacgt taccgcccgc accccgctcc gctgtctcgt
     5281 cctggctgga ccggagctgc atggattcct ggacaagtac ccgaccgtca tgtaccgcat
     5341 gctggtggcc atggctcggc ggctgagcgc cgccaaccag cactccggat agctgcgtgg
     5401 cccgagcgcc tgcgaaggcc actgccgctg atcggccgtt cccgcccgga gcgtacccgg
     5461 tggtggtggt gggcagcggc cccggcgcca tccaggtcag ccacgagctc gatcggcttg
     5521 ggatcgggca tgccacgatc agcgccgacc catcgccagg cgggatgttc cgcagcctgc
     5581 cgatctacca gagactgctg agctggacga agccgtacgc gctcggggat ccggccgggc
     5641 gggccggcga gtggtacgac tggaacagca tcgtctccga cgacccggcg ttgcgcgcga
     5701 tcatgcccgg gctgatggat ggcacctcct ccttcccgag ccgcgccaag atggaggaga
     5761 acctcgccac cttcgtgacg cgggccaacc tgcagattcg ctacggatgc ccctggcagt
     5821 ccaccagccg agcggatgac ggccgcttcg tcctcaccac gccagatggt gactatgtct
     5881 gcgaggtcgc gatcttcgcc gtgggcatgg ctgagccatg gaagccgccg actccggggc
     5941 tcgagctggt gccccactac gtcgaggcag ggccagccga gtcctatgcg gggaagcggg
     6001 tcttcatcgc cggcaagcag aactccgcct tcgagatcgc cacggccttc ctgccgtggg
     6061 ccaggcagct ggtgctggcg tcaccgcgtc cggccacgct gtcagtggtg gagcactcgc
     6121 tggcgggtgt gcgcgcccgc tacgtgcagc cctacgagga cgccatgctg cacaacggcg
     6181 tcttcctgct ggacgtcaag atcgagcgga tcgagcgagc cgacgacggg ttccgggttc
     6241 gcctcagccg gaccgatggc ggccgcgagc tcgtgtgcga ggcggatgcc gcgatcgcca
     6301 ctacgggctt cgcagtgccg atgcgcgacc tgccggccct tggcgtggcc accttcgcgc
     6361 agggcaagat gccggccctc acgccgctgt gggagtcggc cacggtgccg gggatcttct
     6421 tcgccggcac gatcacgcag gccgcagccg ggctcaagaa gcacgggatc ccggcaaact
     6481 ccggggccct gcatggctac cgctataacg cgcgcgtcct ggtgcggcac ctggccgcga
     6541 cccgtttcgg gattgagacc cccagccctg cgatcgaggc cgatgcggtc tcatccttcc
     6601 tgctggaaca gctgaccttg gcccccgagt tgtggaacca gcggtcgtac ctggtgcaag
     6661 tggcatcggt ctccgacgat ggctggcggg acgacgggat ctggcccctg gccgactttg
     6721 tcgacgcctc cggccccgat gccgtcgctg tcaccctcga gccaaatcct gattcgagca
     6781 tctaccccgt cgcctacgtg cggatctcgg gcgccgtcga ggagcacctg ctcgatcccg
     6841 atgccatgaa cgagtaccgc ggctcgcccc atcgggagca gatggccgcg atcctggctc
     6901 ccctcatgcg gtgatgagcg ctgccccagg gcgcatgggt cgatgattcg ccgcctgacc
     6961 gcagccgctg acgcgcttcg gcgcgtcctc gccaaccctg acatgcggcg tgcgcagctg
     7021 gcgtggttgc tcggttacgc cggggagtgg tcgtggctgg tggcgctgtg ggtgtacgcc
     7081 taccagacga gcggcgtcgt tgcggttgga gccctgggtc tcg
//
LOCUS       DATCQR010000198         1542 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_73570, whole genome shotgun sequence.
ACCESSION   DATCQR010000198 DATCQR010000000
VERSION     DATCQR010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73570"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..178
                     /locus_tag="VIN32_06650"
                     /pseudo
     CDS             1..178
                     /locus_tag="VIN32_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611735.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
     gene            complement(185..1333)
                     /locus_tag="VIN32_06655"
     CDS             complement(185..1333)
                     /locus_tag="VIN32_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015780200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEY8922294.1"
                     /translation="MAGILTHEQKQLAFRLRARGWRLVDIAREIGCSAPMVGLMVRAG
                     RFTTGVVDDWQPRQGCLGITEREDILVGLRSGESLAEIARGLGRATSTISREVSGNGG
                     RADYSAWGAHQRARRRARRPKTCKLQHGRLHDLVAHGLQELWSPDEIARRLPLDFPDD
                     PEMRVSHETIYQSLYVQGRGELRRELARCLRSGRTTRKKRGTPDGRGRIPGMVMISER
                     PAEVADRAVAGHWEGDLILGENGRSAVGTLVERTTRLVLLLHLEGGRAAVSVEAAMRK
                     TIATLPEELRCSITWDQGAEMATHANFTTATGIPIYFCDPHSPWQRGSNENTNGLLRQ
                     YLPKGTDLSVHSAADLQRIQRSLNDRPRKTLGYLTPSEAYAQVVAATG"
     gene            1403..1542
                     /locus_tag="VIN32_06660"
                     /pseudo
     CDS             1403..1542
                     /locus_tag="VIN32_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611735.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          236 a          505 c          540 g          261 t
ORIGIN      
        1 tcacgggacc gtgtaccggg tcgcgcatca gctggggtac ggggtcgagt cggtccgggc
       61 gtgggtgcgt caggccgaca tcgacgaggg ccacgtctcg ggggtgagca cgagcgaggc
      121 ggcgaagatg aaggcgctgg agcaggagaa tcgtgagctg cgccgggcca acgagatggc
      181 ggattcaacc ggtggccgca acgacctgtg cgtaggcctc tgatggtgtc aggtatccca
      241 gggtcttgcg gggacggtcg ttgaggctgc gctggattcg ctgcaggtcc gcggcggagt
      301 ggaccgacag gtcggtgccc ttggggaggt actggcgcag cagtccgttg gtgttctcgt
      361 tcgagccccg ttgccacggc gagtgtgggt cgcagaagta gatggggatt ccggtggcgg
      421 tcgtgaagtt ggcgtgtgtt gccatctcgg ctccttgatc ccaggtgatc gagcatcgga
      481 gctcctcggg caacgtagcg atcgtcttgc gcatagccgc ctcgacgctc accgcagcgc
      541 ggccgccctc caggtggagg agcagcacca gccgggtggt gcgctcgacc agggtgccga
      601 cggcgctgcg gccgttctca ccgaggatga gatcgccttc ccagtggccg gctaccgcac
      661 gatcagcaac ctcggctgga cgttcggaga tcatcaccat gccggggatc cgaccgcgcc
      721 cgtcgggggt gccgcgcttc ttgcgggtag tgcgtcctga gcgcaggcag cgggccagtt
      781 cccggcgcag ctcgccccgg ccctggacgt agagcgactg gtagatcgtc tcgtggctca
      841 cccgcatctc cggatcgtcg gggaagtcca acggtaggcg gcgagcgatc tcatcgggcg
      901 accagagctc ctgcaggcca tgggccacca ggtcatgcag acgcccgtgc tggagcttgc
      961 aggtcttggg ccggcgtgcc cggcgcctcg cccgctgatg ggcaccccag gccgagtagt
     1021 ccgcgcggcc gccgttgccg ctcacctctc ggctgatcgt cgaggtcgcc ctccccaggc
     1081 cccgcgcgat ctcggccagc gactcaccgc tgcgaagccc aaccaggatg tcctcgcgct
     1141 cagtgatgcc cagacatccc tgtcggggct gccagtcatc cacgacgccg gtggtgaatc
     1201 ggccagcacg gaccatcaaa cccaccatcg gcgcgctgca gccaatctcg cgagctatgt
     1261 ccaccagccg ccacccccgg gcccggagtc ggaacgccaa ctgcttctgc tcgtgggtaa
     1321 ggatccctgc catggcgctg ctcctgtgat cggcgactcc gaagtgtcac aggaccagtg
     1381 cgctcaccgg ttgaatccgc cgatcctcaa gcgggctgcg tctttcttcg gggcggagct
     1441 cgaccgccaa cacaagaggt agtcgccttc atcgacgcca accgcgacga cgtcgtggag
     1501 ggtcgtcggc tcggggtcga gcccatctgc agcgtgctgc ag
//
LOCUS       DATCQR010000199         1910 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_196631, whole genome shotgun sequence.
ACCESSION   DATCQR010000199 DATCQR010000000
VERSION     DATCQR010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1910
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196631"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(383..1204)
                     /locus_tag="VIN32_06665"
     CDS             complement(383..1204)
                     /locus_tag="VIN32_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014740546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY8922295.1"
                     /translation="MESAKVNGVELEYEVVGSGEPVLLISPVLADGFLPLLSEPTLAD
                     RYQLIRYHKRGWVGSTHTPPPVSIADHAADAAALLDHLGVPRAHIAGHSTGAAVAAQL
                     ALHHPEAVRTLILLELALLSVPSGEAFLQQAGPAFEAYGSGDHEEALAIFMSAASGLD
                     WATCRAVLEERLPGAVAQAIKDADTFFGIELPALTEWAFGPEQAAAIHQPVLSVLGTQ
                     TQPLWVEVADFLRSSLPHVEDCTIDGVGHLLHIQRPEPVARGMAKFLGSNSMAGD"
     gene            1357..>1910
                     /locus_tag="VIN32_06670"
     CDS             1357..>1910
                     /locus_tag="VIN32_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014740545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEY8922296.1"
                     /translation="MSDGDGLGLVERARDAATRGDWQQAFDLLMKADADDLLAPNDLP
                     VLGQVAYAAGHLDVTIEAWERAHAACMQAGDQVAAAGAAVRVAMHLLFDTSLMAPVRG
                     WLARAERLLEGRGETPAHAWFAVVRTYERMLTGDMPDARQWARRAIEVGSKCDPAACA
                     IGRVAEARLLILDGDVQQGLALLD"
BASE COUNT          305 a          594 c          680 g          331 t
ORIGIN      
        1 accctgaagg acaacaccgt ccaggtctgg catatcaagg acggcaaggt gaccgaatcg
       61 tggttgcatc ccggggatgc ctacgcatcc gacgagttct ggtcgtaggc gcggctgcgt
      121 tctccccaac catcgtcaaa cctcgcggag gcgaaggtta cagcgtacga accggggtga
      181 gcctggccca gcgctctggg tgagcagcct ttcctggggc cgagtggcct gagaggtgct
      241 cgttccgcct aagggttaag cgggttgagc ggctccatcg cttttccatc aggcctgatg
      301 gactttcttt gaatgccttc tcgcgacggg gctctgcaaa catctgcgaa cgcgcacgga
      361 tgagggtgga tcagacccgg cgtcagtcgc cggccatgga gttgctcccc aggaacttgg
      421 ccatccctcg tgcgacgggc tcgggacgct ggatgtgcag gagatggccg acgccatcga
      481 tcgtgcagtc ctcgacgtgc gggagtgaag agcgcaggaa gtcggcgacc tccacccaca
      541 ggggctgggt ctgggtgccg agcacggaca ggacgggctg gtggatagcg gctgcctgct
      601 cgggaccgaa cgcccactcg gtgagggcgg ggagctcgat gccgaagaac gtgtcggcgt
      661 ccttgatcgc ctgcgccacc gcgcccggaa gacgctcttc gagcacggct cggcacgttg
      721 cccagtcgag gccgctcgcg gcgctcatga agatggccaa ggcttcctcg tggtcgccgc
      781 ttccgtacgc ctcgaacgca ggtccggcct gctgcaagaa ggcctcgccg ctgggcaccg
      841 agagcagcgc tagctccagc aggatcagcg tgcgcacggc ctcgggatgg tgaagagcca
      901 gctgggcagc gaccgccgcg ccggtggagt ggcctgcgat gtgggcgcgc ggcactccca
      961 gatggtcaag gagcgccgcg gcgtccgcgg cgtggtccgc gatgctcacc ggcggcgggg
     1021 tgtgggtgct gccgacccaa ccccgcttgt ggtatcggat cagctggtag cggtcagcca
     1081 gcgtcggttc tgacaggagc ggcaggaacc cgtcggcgag gaccgggctg atcagcagca
     1141 cgggttcgcc cgatccgacc acctcgtatt caagctctac gccgttgacc tttgcgctct
     1201 ccatgagtcc tccttcggga ttgcctcgtt ggctctggga ctcgtggtcc cctaccgaga
     1261 gtgcgtgagc aatactgtta cggcggtaac cctcacggtt acgttcggcg ccaatggacc
     1321 gcgggctacg atcggccacg gatcgggagg tgtgcgatga gcgatggcga tggtcttggc
     1381 ctcgtcgagc gcgcacgtga cgcggcaacc cggggagact ggcagcaggc attcgacctc
     1441 ctcatgaagg cagacgcgga cgacctcctg gcccccaacg atctaccggt gcttggccaa
     1501 gtcgcctacg cggccggcca tctcgatgtg accatcgagg cgtgggagcg cgcccacgcg
     1561 gcgtgcatgc aggccggtga ccaggtcgcg gcagcaggtg cggccgttcg cgtcgcgatg
     1621 cacctgctct tcgacacttc gctcatggca ccggtgcgcg gctggttggc tcgagcggag
     1681 cggcttctcg aaggtcgggg tgagacgccg gcgcacgcgt ggtttgcggt cgtccgcacc
     1741 tacgagcgga tgctgactgg cgacatgccc gatgctcggc agtgggccag gcgtgccatc
     1801 gaggtgggtt cgaagtgtga cccggcggcg tgcgccatcg gacgggtggc agaggcacgg
     1861 ctgctcattt tggacggcga tgtccagcaa ggtctggcgc tgctcgacga
//
LOCUS       DATCQR010000200         7319 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_42587, whole genome shotgun sequence.
ACCESSION   DATCQR010000200 DATCQR010000000
VERSION     DATCQR010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 7319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7319
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42587"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..459)
                     /gene="ybeY"
                     /locus_tag="VIN32_06675"
     CDS             complement(<1..459)
                     /gene="ybeY"
                     /locus_tag="VIN32_06675"
                     /inference="COORDINATES: protein motif:HMM:NF014216.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEY8922297.1"
                     /translation="MEPQTSEAFHALLESALAETAEAAMGSREVEIELTLCGDEEMTH
                     LNFDRMGERGPTDVLAFPLHEWSVDMGHSHLADDDAGTPPGGLLLGDVVIDVDQAVRQ
                     AVDGGWSPAQEIALLAVHGTLHLVGHDHVEIEDEDAMRAIERRVLAHLHKR"
     gene            complement(548..2656)
                     /locus_tag="VIN32_06680"
     CDS             complement(548..2656)
                     /locus_tag="VIN32_06680"
                     /inference="COORDINATES: protein motif:HMM:NF019317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing metalloprotein"
                     /protein_id="HEY8922298.1"
                     /translation="MFIPRTLSQTESTGRGAWARAAIVGGFLAVALFGVLSINVGTGR
                     VNLSAGDVAQADITAPSTVSFVSDSQTEAARAAAASAVAPVYEQIAPPADIRDRQLVA
                     YDKITRAVRNILLQRDAGTITADEVRQQISSAVPSLADDQVALLGSLPVDNWTLISGA
                     GREVLETTLSGAVRDDQLADARQQVRTNITNDLPQPQRELAGDLAAAQVVPNSQLSAS
                     KTAAAREEARNAVVNVIVKVREGEIVVRTGDPITALDVEKLEQLGLTRPRLEAATLAG
                     HALVAILLALLLVAFLWRFEPTIWFRNRSLLLFMLALLGTAVALRIAADRTLWAYVVP
                     TGATVLLTAILLDGAAGAAMALALATLAGVMNQDAFGPAAYTLAGGLAAVITIVRAER
                     LNVFVRAGVAIGLTNIVVLTAFNLMEQRDVAAILQGLAAGTVNAGLAVFLAVGSFAVL
                     GNLFGIMTVFQLLELANPSSRLLRRLLMETPGTYHHSVMVGNLAERAAETIGADPLLA
                     RVAAYYHDIGKMKNPLAFIENQAGAHNIHDDLTPESSARIIAGHVRDGIDLGYEFGLP
                     VQIIGFIPQHHGTSVMSYFYGKALRELGGDEEVAARDRYRYPGPKPQSREAAILMLAD
                     GVEASVRSLDEKDEVSIRAMVNRIVDARVEDGQLDEAELTLRNLSEIKEAFVQQLLGM
                     YHTRIQYPDNVVPLEHEREA"
     gene            complement(2660..3106)
                     /locus_tag="VIN32_06685"
     CDS             complement(2660..3106)
                     /locus_tag="VIN32_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEY8922299.1"
                     /translation="MTLQDRIETAMRDSMRARDKRRTQTLRMAMSAAHNREIELGKSL
                     SDEDYVEILGRQVKQRRESIEAFRAGGRDAMAANEEAEAAILAEFLPEPLSTEEVEAL
                     VRAAIAETGASSPADLGKVMGKVVPQTRGRADGKAVSDLVRRLLSA"
     gene            complement(3183..3359)
                     /gene="rpsU"
                     /locus_tag="VIN32_06690"
     CDS             complement(3183..3359)
                     /gene="rpsU"
                     /locus_tag="VIN32_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009455158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEY8922300.1"
                     /translation="MAEVRVGENESFESALRRFNKKIQQSGILAEARRREHYEKPSVK
                     RKRKEAKKRKVTRE"
     gene            3598..4152
                     /locus_tag="VIN32_06695"
     CDS             3598..4152
                     /locus_tag="VIN32_06695"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEY8922301.1"
                     /translation="MTMQAGLGVEPVSLNTIIADQAAFQDWYELALPRVYRYLLARCG
                     GDADLAEELTQQTFVEGVRQRASFDGRSDAVTWLCGIGRHKLVDHYRKSRRDTDRQLR
                     IVSEWSAGQSQAWSQHELRSGIETALVKLPGEQRIVMILRYLDQMPVREIASTIGRSE
                     KATESLLSRARDAFRRAYGVQTDE"
     gene            4145..5251
                     /locus_tag="VIN32_06700"
     CDS             4145..5251
                     /locus_tag="VIN32_06700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8922302.1"
                     /translation="MNDEQLTRLFRSLDEQAEPDAAFADTLFADLERMTSGAMARRQT
                     SVRWLLVAATLLLVVSLGAALAVGSGLIKLPFVVDVVSPTPSASATTLGSGTPAPSQS
                     ASTTPTPSPSPAASAIVIDGLAKSTVDGLRVRATPGTAGALLGTIDTGQLGFVVAGPV
                     SADGYAWYQLAALGLPPNAGCEPPFLTTPFNCPDWLGWVAAGQPGSQPWLAATTLPCP
                     ASPMNLEALINEPAPLGPRTALERLACSGSTSIRFRGWWPQNPGGLGGLCGPVVKPNP
                     DWLVCQNINYNGLVISESAGFEGVGMKISIDPATGVTMPPRGQWVEVVGHLDDPAARD
                     CAPLAVGEQDPVKVVLTCRSELVAESVTPVGGPY"
     gene            5276..5710
                     /locus_tag="VIN32_06705"
     CDS             5276..5710
                     /locus_tag="VIN32_06705"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HEY8922303.1"
                     /translation="MSSEDAKLARDIAYGLQAADLGVEIDEELLAAGDSLIDRVAEAT
                     AFVEFVVAVVSTHSIRSNWCKYELALAMTDETLMPRVKLLRVKVGTVEMPPTVRHKVY
                     RSVEQLGAQGVIDALVRDINRHRLRAKDGADATGSAADGPHG"
     gene            complement(5796..6758)
                     /locus_tag="VIN32_06710"
     CDS             complement(5796..6758)
                     /locus_tag="VIN32_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017461343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline iminopeptidase-family hydrolase"
                     /protein_id="HEY8922304.1"
                     /translation="MQGPDRYFDNSGRDDVLSGGVKLVPIHTPKGDFRVWTKRTGNNP
                     RVKVLLLHGGPGATHGYFEACDSYFPAAGVEYYYYDQLGSAWSDQPDDPDLWEIPRFV
                     DEVEQVRQALGLDRDNFYLLGHSWGGMLAIEYALEHQRHLKALVISNMMSSGPAYNAY
                     AERELMPAMDQDALAEIKRLEAANATDDPRYMELLMQHHYVHHILRMPAEAWPDPVNR
                     SFAAINQAIYVPMQGPSELGAGGVLRTWDRTDDLARISVPTLVIGARHDTMDPEFMEM
                     MAGKLQRGTYLFCPNGSHMAMYDDQLTYFNGILDFIRSVEDPRR"
     gene            complement(6779..7099)
                     /locus_tag="VIN32_06715"
     CDS             complement(6779..7099)
                     /locus_tag="VIN32_06715"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEY8922305.1"
                     /translation="MQLKNLDRYGSDSIPWSRALDQLPAKDAPGEDARPRTTWLSTVG
                     PDGRPHTTGVGALWVDDCIYFTIGAKTQKSRNLARDPNCVISLALPDLDLVVEGISAT
                     RWSF"
     gene            complement(7100..>7319)
                     /locus_tag="VIN32_06720"
     CDS             complement(7100..>7319)
                     /locus_tag="VIN32_06720"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HEY8922306.1"
                     /translation="VTVAAGTTVTWKNGDTVPHTATEGSNGVKAPNARFDVQLPVGAS
                     GSFTFTAPGSYQVTCTLHPTMNMTVTVQ"
BASE COUNT         1147 a         2448 c         2457 g         1267 t
ORIGIN      
        1 gcgcttatgg aggtgggcca ggacccgacg ctcgatggcc cgcatcgcat cctcgtcctc
       61 gatctccacg tggtcgtggc cgaccaggtg cagcgtgccg tgcaccgcca gcagggcgat
      121 ctcctgcgcc gggctccacc cgccatcgac cgcctggcga accgcctggt cgacgtcgat
      181 caccacgtcg ccaagcaaca ggccgccggg cggagtgccg gcgtcgtcat cggccaggtg
      241 cgaatgcccc atgtcgaccg accactcatg gaggggaaag gccaggacgt cggtaggacc
      301 gcgctcgccc atgcggtcga agttgaggtg ggtcatttcc tcgtcgccgc acagcgtcaa
      361 ctcgatctcg acttcccggg agcccatcgc cgcctcggcg gtctcggcca gtgccgattc
      421 gaggagcgcg tgaaacgcct cggaggtctg gggctccatc gcagccgtca ggcgatcctg
      481 gctaatccgg tgatggaagg tgacgacgca gcccatggcg ccatcctagg cgcagccggg
      541 gcgaggctca agcctctcgt tcatgctcca gcggcacgac gttatcgggg tactggatgc
      601 gggtgtggta catgcccagc agctgctgga cgaaggcttc cttgatctcc gaaaggttgc
      661 gcagcgtcag ctcggcctcg tcgagctgcc catcctcgac ccgcgcgtcc acgatgcgat
      721 tgaccatggc ccggatcgag acctcgtcct tctcgtccag cgacctgacc gatgcctcga
      781 cgccgtcggc cagcatcagg attgccgcct cgcggctctg cggcttcggc cccgggtagc
      841 gataccggtc ccgcgcggcg acttcctcgt cgccgccgag ctcgcgcagc gccttgccgt
      901 agaagtagct catcacgctc gtgccgtggt gctgcgggat gaagccgatg atctgcaccg
      961 ggaggccgaa ctcgtaaccg agatcgatcc cgtctcgcac gtggccggcg atgatccggg
     1021 cgctcgactc gggggtcagg tcgtcgtgga tgttgtgcgc acccgcctgg ttctcgatga
     1081 aggccagcgg gttcttcatc ttgccgatgt cgtggtagta ggccgccacg cgcgcgagga
     1141 gcgggtccgc gccgatcgtc tcggccgccc gctcggccag gttgccgacc atgacgctgt
     1201 ggtggtaggt ccccggggtc tccatcagca gcctgcggag gagccgcgat gaggggttcg
     1261 ccagctcgag gagctggaag acggtcatga tcccgaacag gttgccgagc accgcgaacg
     1321 agcccaccgc caggaagacg gccagccccg cattcaccgt gccggcggcc aggccctgca
     1381 ggatggccgc cacgtcgcgc tgctccatca ggttgaaggc ggtcagcacg acgatgttgg
     1441 tgaggccgat ggcgaccccc gcccgcacga acacgttcag ccgctcggcc cgcacgatcg
     1501 tgatcacggc agccaggccg ccggcgagcg tatatgcggc tggtccaaat gcgtcctggt
     1561 tcatgacccc ggccagcgtc gccagggcca gcgccatggc cgctccggcg gcgccgtcaa
     1621 gcaggatggc ggtcagcagg accgtcgccc cggtggggac gacgtaggcc cacagggttc
     1681 ggtccgccgc gatgcgcagc gccaccgcgg tccccagcag cgccagcatg aagagcagca
     1741 gggagcggtt gcggaaccag atcgtcggct cgaagcgcca caggaaggcg accagcagca
     1801 aggcgagcag gatcgccacc agcgcgtggc cggcgagggt ggcggcctcc aggcgcggcc
     1861 gggtcaggcc gagctgctcg agcttctcga cgtcgagcgc agtgatcggg tcgcccgttc
     1921 gcaccacgat ctcgccctcg cgcaccttga cgatgacgtt gacgaccgcg ttgcgtgcct
     1981 cttcgcgagc ggccgccgtc ttcgacgcgg acagctgcga gttgggcacc acctgggcgg
     2041 cagccaggtc accggccagc tcgcgctgcg gctggggaag gtcgttggtg atgttggtgc
     2101 gcacctgctg cctggcatcg gccagctggt cgtcgcgcac cgcgcccgag agcgtcgtct
     2161 ccagcacctc gcgccctgca cccgagatga gcgtccagtt gtcgaccggc agcgatccca
     2221 agagcgccac ctggtcgtcg gcaagggagg gaactgccga tgagatctgc tggcgaacct
     2281 cgtccgcggt gatcgtgcca gcgtcgcgct ggagcaggat gttgcggacc gctcgcgtga
     2341 tcttgtcgta cgccacgagc tggcggtcgc ggatgtcggc aggcggggca atctgctcgt
     2401 agacgggtgc gacggcgctg gccgccgctg cacgggccgc ctcggtctgc gagtcgctga
     2461 cgaaactgac cgtgcttggg gcggtgatat cggcctgtgc gacgtcacca gccgacaggt
     2521 tcacccggcc ggtgccgacg ttgatgctga ggacgccaaa gagggcaacg gccaggaacc
     2581 ctccgacgat cgcggcgcgg gcccaggcgc ctcgccccgt ggattccgtc tgcgacagcg
     2641 tcctgggaat gaacatcggt caggcgctca ggaggcgccg cacgaggtca gagactgcct
     2701 tcccgtcggc gcgccccctg gtctgcggga cgaccttgcc catcaccttg ccgaggtcgg
     2761 caggcgagga ggcgcccgtc tcggcgatgg cggcgcgcac gagcgcttcc acttcttcgg
     2821 tggagagggg ctcgggcagg aactcggcca ggatcgcggc ctcggcctcc tcgttggcgg
     2881 ccatcgcgtc gcgaccaccg gcccggaacg cctcgatgct ctcgcgccgc tgcttgacct
     2941 gcctgccaag gatctcgacg tagtcctcgt cggaaagcga tttgccgagc tcgatctcac
     3001 ggttgtgggc agcggacatc gccatgcgca gcgtctgggt gcggcgcttg tcgcgggcgc
     3061 gcatcgagtc gcgcatggcg gtctcgatcc ggtcctggag ggtcatcggc ggatggctgc
     3121 gggcgtcatc ggtctggtcg actcgttgac cggcgcccgc aaattgaggg agcgtctggc
     3181 ggttattcgc gcgttacctt gcgcttcttg gcttccttgc gcttccgctt cacgctgggc
     3241 ttctcatagt gctcgcggcg tcgcgcctcg gccaggatcc ccgattgctg gatcttcttg
     3301 ttgaagcggc gcaatgcgct ctcgaacgac tcgttctcgc ccacgcggac ctctgccaaa
     3361 cgtctcgcct cctctccgct catgaactcg cgcgaacctg cgcgaaagcc tgcggagtct
     3421 atcagcgagc ttgtagaggg tcaatcaacg acggaggtag tagatagagt cgtacgaagt
     3481 tgccgttcgg tcgtgcgtct gcgaaatggg catgcagccc gtccgcggtc acatggcccg
     3541 cgcctatcat gggcaggcga ccgaaggatt tgccgctcgc cgagcgacta attgccaatg
     3601 accatgcaag cgggtctggg cgtggagccg gtgagcctca acaccatcat tgcggaccag
     3661 gccgcgttcc aggactggta tgagctggct cttccgcgcg tctaccgata tctgctggca
     3721 cgctgcggcg gtgacgcgga tctcgccgag gagctgacgc agcagacctt cgtcgaaggg
     3781 gtccggcaac gggcgagctt cgacggccga tccgacgccg tgacctggct gtgcggaatc
     3841 gggcgccaca agctggtcga ccactaccgc aaatcccgtc gcgataccga ccgccagctg
     3901 cgcatcgttt ccgaatggtc ggccgggcag tctcaggcgt ggtcgcagca cgagctgcgc
     3961 tccggcattg agacggcact cgtgaagctc ccgggcgagc aacggatcgt catgatcctg
     4021 cgctacctgg accagatgcc ggtgcgcgag atcgcctcca ccatcggccg atctgaaaag
     4081 gccaccgaat ccctcctctc gcgagcgcgt gacgcgttcc gtcgcgccta tggagtccag
     4141 accgatgaat gatgagcagc tgacccgtct cttccgttcg ctcgacgagc aggcggagcc
     4201 cgacgcggcc ttcgccgata ccctcttcgc cgacctcgag cgcatgacct cgggggccat
     4261 ggctcgccgg cagacatcgg tgcgatggct gcttgtcgcc gccaccctgc tgcttgtcgt
     4321 cagcttgggc gcggcattgg cggtgggcag cggcctgatc aagctgccgt tcgtcgtgga
     4381 cgtcgtctcg ccgaccccgt cggcaagcgc caccacactg ggcagcggca ctcccgcccc
     4441 cagccaaagc gcatcgacaa cgccgacccc cagcccgtca cccgccgcca gcgcgattgt
     4501 gatcgacggc ctcgccaagt cgaccgtcga cggtctgagg gtccgcgcca cgccgggtac
     4561 cgcgggtgcc ctcctcggga cgatcgacac gggtcagctg gggttcgtag tggctggacc
     4621 ggtcagcgcc gatgggtacg cgtggtacca gctggcggct cttgggctgc cgccgaacgc
     4681 gggttgtgag ccacccttcc tgaccacccc gttcaactgc cctgactggt tgggctgggt
     4741 ggctgccggc cagcccggca gtcagccctg gctcgcggca acgacgctgc cctgtccggc
     4801 atcgccgatg aatctcgaag ccctcatcaa tgagcccgcg ccgctgggac cccgcaccgc
     4861 gctcgaacgg ctggcatgct ctgggtccac ctcgatccgc ttccgcgggt ggtggcccca
     4921 gaaccctggg gggcttggcg ggttgtgtgg gcccgtggtg aaacccaacc ccgattggct
     4981 tgtctgccag aacatcaact acaacggact ggtcatctcg gagagcgctg ggttcgaggg
     5041 tgtcggaatg aagatcagca tcgatcccgc caccggtgtg acgatgccgc ctcgcggaca
     5101 atgggtcgag gtggttggcc acctggacga tccggccgct cgcgactgcg ccccgctcgc
     5161 agtcggagag caagatccgg tcaaggtggt gctgacctgc cgcagtgagc tcgtcgcgga
     5221 gtccgttacg ccggtcggcg gaccgtactg atcggccggc acctgacctg agccgatcag
     5281 ctccgaggac gccaaactcg cccgcgacat tgcctacggc ctgcaagcgg ccgacctagg
     5341 cgtcgaaatc gatgaggagt tgttggcggc cggcgactcg ctcatcgacc gcgtagccga
     5401 agccactgct ttcgtcgaat tcgtggtggc cgtcgtttcc acacactcca ttcggtcgaa
     5461 ttggtgtaag tacgagctgg cgcttgcaat gaccgatgag acgctcatgc cacgtgtaaa
     5521 gctgctccgc gtcaaggtcg ggactgtgga aatgccgcca acggtgcggc acaaggtcta
     5581 ccggagcgtc gagcagctcg gcgcgcaagg cgtgatcgat gctttggttc gcgacatcaa
     5641 caggcaccgc ctgcgggcga aggacggtgc tgacgcgacg ggatctgccg cagacgggcc
     5701 tcatggatga gcacttacaa ggtcattgct gatccctggg attgccctcc ccccagaccg
     5761 gcaacttcag cgggctctat ctactacccc gtcaatcaac gacgggggtc ctcgacgctg
     5821 cgaatgaagt cgaggatgcc gttgaagtag gtgagctggt cgtcgtacat cgccatgtgg
     5881 ctgccgttgg ggcagaacag gtacgtgcca cgctgcaact tgcccgccat catttccatg
     5941 aattcgggat ccatcgtgtc gtggcgagcg ccgatcacca gcgtcgggac cgagatgcgg
     6001 gcgaggtcgt cggtccgatc ccaggtgcgc aggacgccgc cagcaccgag ctcgctcggg
     6061 ccctgcatcg gcacgtagat ggcctggttg atggcggcga agctgcggtt caccgggtcc
     6121 ggccatgcct cggccggcat gcgcaggatg tggtgcacgt agtggtgctg catgagcagc
     6181 tccatgtagc gcgggtcatc ggtcgcattc gccgcctcga ggcgcttgat ctccgccagc
     6241 gcgtcctggt ccatcgccgg catcagctcg cgctcggcat aggcgttgta cgccgggccg
     6301 ctgctcatca tgttggagat cacgagcgcc ttgagatggc gctggtgctc gagcgcgtac
     6361 tcgatcgcca gcatgccgcc ccacgagtgc ccgagcaggt agaagttgtc gcggtcgagg
     6421 ccgagcgcct ggcggacctg ctccacctcg tcgacgaacc gggggatctc ccacagatcc
     6481 gggtcgtcgg gctggtcact ccatgccgag ccgagctggt cgtagtagta gtactcgacc
     6541 ccggcggcag gaaagtacga gtcgcacgcc tcgaagtacc cgtgcgtggc accggggcct
     6601 ccgtgaagca ggaggacctt cactcgcggg ttgttgccgg tccgcttggt ccacacccgg
     6661 aaatcgccct tcggcgtgtg gatcgggacc agcttcacac cgcccgagag gacgtcgtcc
     6721 cggccagagt tgtcgaagta gcggtccggt ccctgcacct cgatccttcg ccgggggctc
     6781 agaagctcca gcgcgtggcg ctgatccctt ccaccaccag gtccagatca ggcagagcga
     6841 gcgagatcac gcaattcggg tcgcgcgcca ggttccgact cttttgcgtc ttcgcgccga
     6901 tcgtgaagta gatgcagtcg tcaacccaca gggcgccgac accggtcgtg tgcggccgcc
     6961 cgtcgggccc cacggtgctc agccaggtcg tcctcgggcg tgcatcctcc cccggcgcat
     7021 ccttcgcggg gagctggtca agtgcgcgcg accacggaat cgagtcggat ccgtaccgat
     7081 cgaggttctt cagctgcatc tattgcaccg tgacggtcat gttcatggtg gggtgcaacg
     7141 tgcaggtcac ctggtatgag cccggggccg tgaacgtgaa gcttcccgac gccccgaccg
     7201 gcagctggac gtcgaagcgc gcattgggcg ccttgacccc gttcgacccc tcggtggcgg
     7261 tgtgcgggac ggtgtcgcca ttcttccacg tgacggtcgt gccggccgct accgtgaca
//
LOCUS       DATCQR010000201         9109 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_42715, whole genome shotgun sequence.
ACCESSION   DATCQR010000201 DATCQR010000000
VERSION     DATCQR010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 9109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9109
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42715"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(165..902)
                     /locus_tag="VIN32_06725"
     CDS             complement(165..902)
                     /locus_tag="VIN32_06725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8922307.1"
                     /translation="MSSAIGFDPTRLLAAWEDAGRRRPPWRQLAMAAAFLGEPVETLR
                     EITPGRLEAALLDFYEAAFGPTLDGTVRCEACGEWLEVSVEIPAIRIGSAAGPPELLA
                     KLGRYEVCCRLPQVADLAQLAAATDVAEGRRLLLQRCLRWARRDGRAVPEGALPERVV
                     RLVSARMAEADPQADLALEVLCAACGSRGQALLDVGLLAWHKLNAWAQRLLLQVHILA
                     AAYGWREADILELSPARRRTYLELAQA"
     gene            complement(899..1423)
                     /locus_tag="VIN32_06730"
     CDS             complement(899..1423)
                     /locus_tag="VIN32_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018078747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEY8922308.1"
                     /translation="MAEFTVNSQRFDPYKNFKFRVKWDGRYVAGISKVGGLKRSTEVV
                     KHRTGGDPSTTRKSPGRTEYDPITLERGVTHDTEFEKWANKVWNFGSGLGAEVSLKDF
                     RKDVILELYNEAGQVAIAYQIYRCWVSEWQSLPDLDANANAVAIQHIRLENEGWERDY
                     AVTEPQEPTFTEPA"
     gene            complement(1448..3838)
                     /locus_tag="VIN32_06735"
     CDS             complement(1448..3838)
                     /locus_tag="VIN32_06735"
                     /inference="COORDINATES: protein motif:HMM:NF016845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath C-terminal domain-containing
                     protein"
                     /protein_id="HEY8922309.1"
                     /translation="MPVTPTFPGVYIEEIPSGVRTIVGVATSIGLFVGRARQGPLNQP
                     VLCLNFSDFERAFSSDYARSDLARSVRLFFQNGGSQCYVVRIASGATQSTVTLENTAG
                     NPVLVATSKSAGAVGDTMRLAVTYSGPRPEATFNLEVFRWTQTPQGVQVKADRELYQN
                     LDMDPLSPRYAPDYVTQNSALITLAAQGGLTPAANGFSQSGRAVPARTNAIFQTQWKA
                     LIGTTSTPNRFRISVDGGAFTEVDLSTLDFTQAPLSTVANIATSLGTAIKQLIEAKIQ
                     SGAQVTVSFPNGPAGTAGQDNEQTVTMRIASANGDVLIQPASVNDTAIALLLGTDQGG
                     IEVGRFAQNRPAPTGTVFNPGNNLVNLVNFMAQKQTAFNTINVAGTAIALAGFQTVVG
                     PEARMYQDGFTTAVTGNSDGVREKLSMIVAAVNAQQAADPTFKWSAEAWGDRLALLPG
                     AGGDNSQGAVTTSGGDGVNIATSFTPNVRYYSLGPSGTGTWQVPAGAPASDGSAPAMS
                     DYRAAYGVVDSQVDLFNLLVLPSDEDHLPATRASLWGPASDFCVKRRAFLMMDAPAEW
                     KTVTQATAGITALRVGLAKDHAAIYHPRIVINENGRITSVGAGGAIAGLMSRIDGSRG
                     VWKAPAGMEADLRGIVDVDIKYSNDENGILNPRAINTIRSFPAGVVSWGARTMDGDDG
                     FGSEYRYVPIRRLALFIEESLYRGTQWVVFEPNDEPLWSQIRLNVGAFMHNLFRQGAF
                     EGKTPLQAYFVKCDGETTTANDRNLGIVNILVGFAPLKPAEFVIIQIQQIAGQIQT"
     gene            complement(4095..4286)
                     /locus_tag="VIN32_06740"
     CDS             complement(4095..4286)
                     /locus_tag="VIN32_06740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8922310.1"
                     /translation="MEQPEEDFVVIVIRAWLQEGHSDALRARITAWRADGHQRVRVAS
                     TPEAVCEAVTTTLDELLPA"
     gene            4534..5208
                     /locus_tag="VIN32_06745"
     CDS             4534..5208
                     /locus_tag="VIN32_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005159998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HEY8922311.1"
                     /translation="MKGFELGCGGRPISLPLGSQRLLAFLALHERPLVRSYVAGTLWQ
                     DATEDRSNACLRSTLWRLRNRGYPLVEATGDHVCLADQVRVDSREAATQARQVIQGTD
                     DYRPSEVQRMLMVGDLLPDWYDDWLVIERERLRQLRLHALEALAVRLTAMGRFAEAVE
                     AALGAVMAEPLRESAHMALIAAHLAEGNRGEALRQFRACRDLLRRELGLEPSPRLQEA
                     VRGLGI"
     gene            complement(5264..7312)
                     /locus_tag="VIN32_06750"
     CDS             complement(5264..7312)
                     /locus_tag="VIN32_06750"
                     /inference="COORDINATES: protein motif:HMM:NF013713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine synthase"
                     /protein_id="HEY8922312.1"
                     /translation="MSSTAAGEATVLRTVATRTARWLPRSVRLFLLSFLMLFLELALI
                     RWTGSKVLYLSYFSNFVLLASFLGIGAGFLRAHSSRDLFQFGPLLLAVLVGFVLVFPV
                     QIERSGTEIIFFGALQQSRSGLPIWVTLPAIFVVVAAVMMTVAEGVGRTFVEFEPLQA
                     YRLDIMGSIAGIAAFSLLSFADVPPIGWALVVGSLMLLLLAPVIRRFQVISVAVLLLT
                     LGIESVLPLESWSPYYRITLGPQLSGAISISVNGIPHQTIESVEQRRQSVPLYFLPYS
                     RLGARPSNVLIVGAGTGNDVAIALAQGVQHVDAVEIDPSLHDLGVRLHPDHPYQDPRV
                     TSYIDDGRAFLQQTDHRYDLILFALPDSLTLVAGQSSLRLESYLFTVEAMKQARAHLK
                     PGGMFGEYNYYREQWLIDRLAGTLQSVYGHPPCLDSTGVTGKLALLSASVDPSSIDCP
                     AAWNPASRVVPAPATDDYPFLYLQQRGVPSFYLLMLGLIVLAAFALVRMAGGPLQGMA
                     GYGDLFFMGVAFLLLETKSVVQFALLFGTTWFVNALVFTGILLAVLAAVTLAQRVRRQ
                     SMWLLYGALLAALVVAWLVPPESLLRLSLLPRFLAASGLAFVPIFVANLVFAQRFSAV
                     ASSNVAFAANLLGAMIGGVLEYSSLALGYRALLPVIAGLYIVALVLWLRGRPQAGLQT
                     "
     gene            complement(7365..7679)
                     /locus_tag="VIN32_06755"
     CDS             complement(7365..7679)
                     /locus_tag="VIN32_06755"
                     /inference="COORDINATES: protein motif:HMM:NF024934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY8922313.1"
                     /translation="MALAEEVTNRLVDAYRTLVSTAFATVRSRVARDLLERAGRTGRT
                     RKSDHLRVTQQALADATGSVREVVARALRELRQQGAIETDQAGVTILNVDALIAEAGH
                     SL"
     gene            complement(7772..8080)
                     /locus_tag="VIN32_06760"
     CDS             complement(7772..8080)
                     /locus_tag="VIN32_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8922314.1"
                     /translation="MAHMPSRDHLAGSHADALAAIETNGFLARLSPELAGNLIRSANL
                     VYYPGGSISDPARDAAWAAVVVSGVVRQYLPMQDGRQVTVGYAEAGDLVGARTEVVVG
                     "
     gene            complement(8339..>9109)
                     /locus_tag="VIN32_06765"
     CDS             complement(8339..>9109)
                     /locus_tag="VIN32_06765"
                     /inference="COORDINATES: protein motif:HMM:NF037653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY8922315.1"
                     /translation="EAELRLKRDLVDALLDGTDDETLQSRAQGLGYDLGRPHRVVVAE
                     SRGTTRADDTFFQAVRRAARDLSIGTLIVARRGAVVVLASRDVPWEDFRQAILRGLED
                     GRCRLGIGGRCSTPSEFARSHHEAQLALRMERSSGLDQVSTFDDLGVYRLLSGIEDPA
                     DVERFVRERIGKLLDHDAQRNSELVRTLTHYLECGGSYERTSAAIFVHRSTLKYRLQQ
                     IRDITGYDLNDPDTRFNLQLSTRAWGTLRALREGSPSP"
BASE COUNT         1487 a         2961 c         3242 g         1419 t
ORIGIN      
        1 ccggccggca ggcgcagcct cgcggacatg ccgccactcc gccgcatctc gtccgatgct
       61 gacggcaagg gcgggccccg gcggcggttc gtagatggaa ccggcgcgag gcagcacccg
      121 gccgcggccg agcgcgcggg cggccaggtt ggccaggtag tcgctcatgc ctgggccagc
      181 tccaggtaag tccggcggcg ggcagggctc aactcgagaa tgtccgcttc tcgccagcca
      241 taggcggcgg ccaggatgtg gacctggaga aggagccgct gcgcccaggc gttgagcttg
      301 tgccacgcca gcagccccac gtcgaggagc gcctgcccgc ggctgccgca agcggcgcag
      361 agcacctcaa gggcgaggtc cgcctggggg tcagcctccg ccatgcgggc cgagaccagc
      421 ctcacaaccc gttcggggag cgcgccctcc ggaacggccc gaccgtcccg ccgcgcccaa
      481 cgcaggcacc gctggaggag gaggcggcgg ccttcggcca cgtcggttgc ggctgccagc
      541 tgcgccaggt cggccacctg cggcagccgg caacagactt catagcggcc cagtttggcc
      601 agcagctcag gcggaccggc agccgatccg atccggatgg cggggatctc gacgctgacc
      661 tccagccact cgccgcaggc ctcgcagcgg accgtgccgt cgagggtggg cccgaaggcg
      721 gcctcgtaga aatcgagcag ggcggcctcg aggcggccgg gcgtgatctc gcgcagcgtc
      781 tccaccggtt cacccaggaa ggccgcagcc atggccagct ggcgccacgg gggacgccgg
      841 cgcccggcat cctcccaggc ggccagcagc cgcgtcgggt cgaaaccgat ggcactgctc
      901 atgccggttc ggtgaaggtc ggctcctgcg gctcggtcac cgcgtagtcg cgctcccagc
      961 cctcgttctc gagcctgatg tgctggatcg cgacggcgtt cgcgttggcg tcgaggtcgg
     1021 gcagcgactg ccactcggag acccagcagc ggtagatctg gtaggcgatc gccacctgcc
     1081 cggcctcgtt gtagagctcc aggatgacgt ccttgcggaa gtccttcaga gatacctcgg
     1141 cgcccagccc ggaaccgaag ttccagacct tgttggccca cttctcgaac tcggtgtcgt
     1201 gggtcacgcc gcgctcgagg gtgatggggt cgtactcggt ccggcccggc gacttgcggg
     1261 tcgtgctcgg atcgccgccg gtgcggtgct tgacgacctc agtgctgcgc ttgaggccgc
     1321 cgaccttgct gatcccggcg acgtaacggc cgtcccactt gactcggaac ttgaagttct
     1381 tgtagggatc gaagcgctgg ctgttgacgg tgaattcggc cacggctctc ttcctttcct
     1441 ccccgaccta cgtctggatc tggccggcga tctgctggat ctggatgatc acgaactccg
     1501 ccggcttcaa cggcgcgaag ccgaccagga tgttgacgat gcccaggttc ctgtcgttgg
     1561 cggtggtcgt ctcgccgtcg cacttcacga aataggcctg gagcggggtc ttgccttcga
     1621 atgcgccctg ccggaacagg ttgtgcatga aagcgccgac gttgagccgg atctgcgacc
     1681 agagcggttc gtcgttgggc tcgaacacga cccactgcgt gccgcggtag aggctctcct
     1741 cgatgaagag cgccagccgg cggatgggga cgtacctgta ctcgctgccg aagccgtcgt
     1801 cgccgtccat cgtgcgggca ccccagctga cgacgccggc cgggaagctg cggatggtgt
     1861 ttatggcccg tgggttgagg atgccgttct cgtcgttcga gtacttgatg tcgacgtcga
     1921 cgatcccgcg caggtcggcc tccatccccg ccggcgcctt ccagacgccg cggctgccgt
     1981 cgatccgtga catcaggccg gcgatcgcac cgccggcgcc gacactggtg atgcggccgt
     2041 tctcgttgat cacgatgcgt gggtggtaga tggccgcatg gtccttggcg aggccgacgc
     2101 ggagcgccgt tatgccggcg gtggcctgcg tcacggtctt ccactcggcc ggcgcgtcca
     2161 tcatcaggaa ggcccgacgc ttgacgcaga agtcgcttgc cgggccccag agcgaggcgc
     2221 gggtggcggg cagatggtcc tcgtcggacg gaagcacgag gaggttgaag aggtcgacct
     2281 ggctgtcgac gacaccgtac gcggcgcggt agtcgctcat ggcgggcgcc gaaccgtcgc
     2341 tggccggtgc gccggcgggc acctgccagg tgcccgttcc ggacggcccc aggctgtagt
     2401 agcggacgtt gggcgtgaag gaggtggcga tgttgacgcc gtcgccgcca ctggtcgtga
     2461 cggcgccctg gctgttgtcg ccgccggcgc cggggagaag ggcgaggcgg tcgccccagg
     2521 cctcagccga ccacttgaag gtcgggtccg ccgcctgctg ggcattgacc gctgcaacga
     2581 tcatgctcag cttctcgcga acgccatcgc tgttgccggt caccgcggtc gtgaaaccgt
     2641 cctggtacat gcgggcctcc ggccccacca ccgtctggaa cccggccagc gctatcgccg
     2701 tgcccgcgac gttgatggtg ttgaaggcgg tctgcttctg agccatgaag ttgacgaggt
     2761 tgaccaggtt gttgccgggg ttgaagacgg tcccggtcgg agcgggtcgg ttctgggcga
     2821 agcggccgac ctcgatgcca ccctggtcag tccccaagag cagggcgatc gctgtgtcgt
     2881 tgacggatgc gggctggatg agcacgtccc cgttcgccga agcgatacgc atcgtcaccg
     2941 tctgctcgtt gtcctggccg gctgttccgg cgggtccgtt ggggaagctc acggtgacct
     3001 gggcgccgct ctggatcttc gcctcgataa gctgcttgat cgcggtgccg aggctggtgg
     3061 ctatgttcgc cacggtgctg agcggggcct gggtgaagtc gagggtcgag aggtcgacct
     3121 cggtgaaggc gccgccgtcg acgctgatgc ggaagcggtt gggggtgctg gtcgtgccga
     3181 tcagcgcctt ccactgggtc tggaagatgg cgttggtgcg ggctggaacc gcacggccgg
     3241 attgcgagaa gccgttcgcg gccggtgtca gcccgccttg ggcggcgagc gttatcagcg
     3301 ccgagttctg cgtcacgtag tccggcgcgt agcgggggct gagggggtcc atgtcgaggt
     3361 tctgataaag ctcgcggtcg gccttgacct gcacgccctg gggagtctgc gtccaccgga
     3421 agacctcgag gttgaaggtt gcctccggcc ggggaccgga gtaggtgacg gccagccgca
     3481 tggtgtcgcc gacggcgccc gccgacttgg aagtggcgac caggaccggg ttgcccgcgg
     3541 tgttctcgag cgtgaccgtc gactgggtcg cgccgctcgc gatgcggacg acgtagcact
     3601 gggagccgcc gttctggaag aagaggcgca ccgagcgagc gaggtcgctg cgtgcgtaat
     3661 cggaggagaa ggcgcgttcg aagtcgctga agttgaggca tagcacgggc tggttgagtg
     3721 ggccctggcg ggcacggccc acgaagagtc cgatcgaggt ggcgacgccg actatggtcc
     3781 ggacgccgct cggtatctcc tcgatataga cgccggggaa ggtcggcgtg actggcatgg
     3841 acgctcctcc tatatccgtg cggcggctgc gggcttgcgg aaacaggtcg ccccgaagcc
     3901 ggctcgtcgg accatggccg acagggtgaa caccgaccat cactaggccg tcacgcccac
     3961 atcacgccca catcacgccc gcgtcacagc cgcctcgacg cggagacgcg gcggtggcgg
     4021 cccctgtcac cttcctcaac cgtggctacc ggcggcgacc gcgcggttcc gggagctgcc
     4081 ctggtcccct cagcctaggc ggggagcaac tcgtccagcg tggtggtcac cgcctcgcac
     4141 accgcctcgg gcgtcgaggc gacgcgcact cgctggtggc cgtcggcgcg ccaggccgtg
     4201 atccgagctc gcagtgcatc gctgtggcct tcctgcagcc aggcccggat gacgatgacg
     4261 acgaagtcct cctcaggctg ctccacgact caggcgcaaa gtgccgatga ggctgcgctg
     4321 acgtccattc ggcgcacgtt agagtggcgc ccgtcaccac cgcatcacca tttccggacg
     4381 ctgtcaactc aacgacttga caccgctgga agaacgacca taatcggctt ctcaaagcca
     4441 gtcgccatag gccgggggga catgggtatg gtcggtagct caccgacgac ggggagaccg
     4501 tggtcggcgg agccgcgtcc agagctcagg ctaatgaagg gtttcgagct cgggtgtggg
     4561 ggccgcccca tctccctgcc gctgggcagc cagaggctgc tcgcattcct tgccctgcac
     4621 gagcgcccgc tcgtccgctc ctacgtggcc ggcaccctct ggcaggacgc gaccgaggat
     4681 cgctccaacg cctgtctgcg ttcgaccctc tggcggctcc gcaaccgggg atacccgctg
     4741 gtggaggcga ctggcgacca cgtctgcctc gccgaccagg tgcgggtgga cagtcgagag
     4801 gcggcgaccc aagctcgcca ggtgatccag ggaaccgacg attaccggcc ctcggaggtc
     4861 cagcggatgc tgatggtcgg cgaccttctg cccgactggt acgacgactg gctcgtcatc
     4921 gagcgcgaga ggttgcgcca gctgcggctc cacgccctcg aggcgctcgc cgtccgcctc
     4981 accgcgatgg gccgcttcgc ggaggcggtc gaggcggccc tcggcgcggt aatggcggag
     5041 cccctccgcg aaagcgccca catggcgctg atcgcagccc acctcgccga ggggaaccgg
     5101 ggcgaagccc tacgccaatt ccgcgcctgc cgggacctgc tcagacgcga gctcggcctg
     5161 gagccgtcgc cgcgcctgca ggaggcggtg cgcggcctcg ggatctgacc ctgcatacgt
     5221 gcgacagagg ccagccccca accgaagccc ttctgctgtc tcttcatgtt tgcagtccag
     5281 cctgcggccg gcctcgtagc cacagcacca gcgccacgat gtagagcccg gcgatgaccg
     5341 gcaacagcgc ccggtagccg agcgcgaggg agctgtattc gaggacgccg ccgatcatcg
     5401 ccccaagcag gttggcggcg aacgcgacgt tcgaggaggc gacggcgctg aaccgctgcg
     5461 cgaagaccag gttggcgacg aagatgggaa cgaaggccaa cccgctggcg gcgaggaatc
     5521 gcggcagcag cgaaagccgc agcagcgact ccggcggtac cagccaggcg acgacgaggg
     5581 ccgcgagcag cgcgccgtag agcagccaca tgctctggcg acgaacgcgc tgcgccaacg
     5641 tcaccgcggc cagaacggca agcaggatgc cggtgaagac gagggcgttc acgaaccagg
     5701 tggtgccgaa gaggagcgcg aactgcacga cgctcttcgt ctccagcagc aggaaggcga
     5761 cgcccatgaa gaataggtcg ccatacccgg ccatgccctg gagtgggccg cccgccatgc
     5821 gcaccagcgc gaaggccgcg agcacgatca ggcccagcat cagcaggtag aagctgggga
     5881 ctccgcgctg ttgcaggtag aggaaggggt agtcgtccgt cgccggcgcc ggcacgacgc
     5941 gggaggcggg attccaggcc gcggggcagt cgatggaaga cggatcgacg cttgcgctga
     6001 ggagcgccag cttgccggta acgccggttg aatccaggca gggcggatgg ccgtaaaccg
     6061 actggagggt gccggccagc cggtcgatga gccactgctc gcggtagtag ttgtattcgc
     6121 cgaacattcc gcccggcttc agatgcgcac gtgcctgctt catcgcctcg accgtgaaca
     6181 ggtagctctc gagccggagg gaggactgcc ccgccaccag ggtcaaggag tcgggcaggg
     6241 cgaagaggat caggtcgtag cggtggtcgg tctgctgcag aaacgcgcgc ccgtcgtcga
     6301 tataggaagt gacgcgaggg tcctggtagg ggtggtcggg atgcagtctg acgccgaggt
     6361 cgtggagtga gggatcgatt tcgaccgcgt cgacgtgctg aacgccctgg gcgagcgcga
     6421 tcgcgacgtc gttgccggtg ccggcaccga cgatcagcac gttgcttggt cgggctccca
     6481 ggcgggagta aggcaggaag tagagaggaa ccgactgccg ccgctgctcg accgattcga
     6541 tcgtctggtg ggggatgccg tttacggaga tcgagatggc gccgctgagc tgaggaccga
     6601 gggtgatccg gtagtagggc gaccacgact cgaggggcaa caccgactcg atcccaagcg
     6661 tcagcaacag cacggccacc gagatgacct ggaagcgcct gatcaccggc gcgagcagga
     6721 gcagcatcaa gcttcccacg accagcgccc atccgatggg cggcacgtcg gcaaaggaga
     6781 gcagggagaa cgcggcgatg cccgcgatgc tgcccatgat gtcgagccgg tatgcctgga
     6841 gtggctcgaa ctcgacaaag gttcggccga ctccctcggc gaccgtcatc atcaccgcag
     6901 cgacgacaac gaagatcgcc ggcagggtga cccagatcgg gagtccgctg cggctctgct
     6961 gcagcgcacc gaagaagatg atctcggtgc ccgaccgctc gatctggact ggaaagacga
     7021 ggacgaatcc gaccagaacg gccagcagca gcggcccgaa ctgaaagagg tcgcgcgagg
     7081 aatgggcgcg caggaagccg gcgccgatgc cgaggaagct cgcgagcagc acgaagttcg
     7141 agaagtagga gagatagagg accttggaac ccgtccagcg gatgagcgca agctcgagga
     7201 agagcatcag gaagctcagg agaaaaagcc ggacgctcct cggcagccac ctcgccgttc
     7261 gcgtggccac cgtccgcagg acggttgcct cgcccgccgc cgtcgaactc actccctgga
     7321 gggtagtggc ttgcgccggc gaagcgatgg tccgcgacgg gacctcagag actgtggccg
     7381 gcctcagcga tcagtgcgtc gacgttgagt atggtgacgc ctgcctggtc ggtctcgatg
     7441 gcaccctgct gccggagctc gcggagggcg cgcgccacca cctcgcggac cgaaccggtg
     7501 gcgtcagcca gggcctgctg agtgacccgc aggtggtcgc tcttccgtgt tctccccgtc
     7561 cttcccgctc gctcgaggag gtcgcgggcc acgcgtgacc ggaccgtggc gaaggccgtg
     7621 ctgaccaacg tccggtaggc gtcgacaagg cggttggtca cctcctcggc cagggccatc
     7681 gacaggtccg gctcacgctg ggcagttcgc tccaggagag ccgcgtcgag gtggaggagg
     7741 taggcgggct caatcgcctc gatctcgggc ctcagccgac gactacctct gtacgggccc
     7801 ccaccaggtc gccggcttcc gcatagccaa cggtgacctg acggccgtcc tgcatcggaa
     7861 ggtactggcg gaccaccccg gagaccacga ccgctgccca ggcagcatcg cgtgcagggt
     7921 cactgatact gcctccgggg taatagacga gattggccga gcggatgagg ttgccggcga
     7981 gctcaggtga caggcgcgcc aggaagccat tggtttcgat ggccgcgagt gcgtccgcgt
     8041 gactgccggc caggtgatcg cgcgacggca tgtgcgccac ggccaatcgc ctagtgcccc
     8101 gacgacggcg gtcgatcgcc gtttgacgga tgcacttcga gcgggcggtc gcgccggatt
     8161 cggggaggct ggcgcatggt ggccggatcg tggcaccggc ggttcgaaac cgtcatgtcc
     8221 cgccaggacg aagtgatggg ccgctgtcgt cacggcgtga ctacaccgct gatgcccatc
     8281 tcgcctcgat gggtcgaacg agaaggcggg cgagggctga ccgcgaccag ccgtctcgtc
     8341 aggggctcgg gctgccctct cgaagcgcac gcaacgtgcc ccaggccctg gtgctcagct
     8401 gcaggttgaa gcgcgtgtcg gggtcgttga gatcgtagcc ggtgatgtcg cggatctgct
     8461 gcaggcggta cttcagcgtg ctgcggtgca cgaagatggc cgcgctggtg cgctcgtagc
     8521 ttccaccaca ttccaggtag tgcgtgaggg tacggaccaa ttcgctgttt ctctgggcgt
     8581 cgtggtcgag cagcttgccg atccgctcgc ggacgaagcg ctccacgtcc gcgggatcct
     8641 cgataccgga gagcagtcga tacacaccga gatcgtcgaa cgtcgacacc tggtcgaggc
     8701 ccgaggagcg ctccattcgg agcgcgagct gggcctcgtg atgcgatcgc gcgaactcgg
     8761 acggcgtgct gcaccgcccg ccgatgccca gccgacagcg gccatcttcg agcccgcgga
     8821 ggatggcttg gcggaagtcc tcccagggca cgtcgcgact ggcgagcacg accacggcgc
     8881 cccggcgagc gacgatcagg gtgccgatcg acaagtctcg ggcggcgcgg cgcaccgcct
     8941 ggaagaaggt gtcatccgcc cgcgtcgtgc cacggctctc cgcgacgacc acgcggtgcg
     9001 gacgtccgag gtcatagccg aggccctggg cccggctctg caacgtctcg tcatcggtgc
     9061 cgtcgagaag cgcgtcgacc aggtcccgct tcagtcgcag ctcggcctc
//
LOCUS       DATCQR010000202         1892 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_73925, whole genome shotgun sequence.
ACCESSION   DATCQR010000202 DATCQR010000000
VERSION     DATCQR010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1892
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73925"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1167
                     /locus_tag="VIN32_06770"
     CDS             <1..1167
                     /locus_tag="VIN32_06770"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY8922316.1"
                     /translation="ALERETAVGSVLQSISRSTFRLDEVLQTVIDSATRLSHADEGNI
                     LREESGRFRLAAYTAGVPEDFRRILNRLVFQPDRGTIVGRVLMERGPVHIVDIVADAE
                     YRLKEAQEASGFRTVLGVPLVRDGAPIGVLVVWRLQVQPFTDPEIALLTTFADQAALA
                     IASVTLFETIDRQRTELARYAPQAAELLSSVEGEQLLAGHRREITALFADLRGFTAFA
                     EQAEPEEVLGVLRQYHEAVGELAVANGGTVEHFAGDGLMVFFNDPQPVADHQLAAVRT
                     ACDMRERFGVLSSAWRKRGYELGLGIGIASGFATLGRIGFEGRYDYAAIGNSVILASR
                     LSDAASAGQILISQRVLAVIEEAVVAEPVEDLTLKGFSHALKAFAVESMRVPAA"
     gene            complement(1164..>1892)
                     /locus_tag="VIN32_06775"
     CDS             complement(1164..>1892)
                     /locus_tag="VIN32_06775"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: protein motif:HMM:NF013461.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HEY8922317.1"
                     /translation="IHGAVVKPDAYLENVSQKEPDGPVDFFDTSYTKNGRATFPMASL
                     GIWRDPREIGPVNHLLILNRNDNVIPAVARLSRVQAAAYFMLGETQGTSAGGAAEEGR
                     ALRVPGTNPFYPYRDEQQANRFLELMASSDFDVFLLNTGRVGGVEDNPSSKKVAIEHS
                     GAIVKAIAEGTIEWERDPDFGYLVASVLPGVDDLELLQPRLLYERTGRADEYRRWVER
                     LKRERIEFLEGYSGLAPEIVAAVR"
BASE COUNT          299 a          635 c          635 g          323 t
ORIGIN      
        1 gcgctcgaac gggagacggc ggttggcagt gtcctgcagt cgatcagccg ctcgaccttc
       61 cgtctcgacg aggtccttca gaccgtcatc gacagcgcca cgcgcctgtc gcacgcggac
      121 gaaggcaata tcctccgcga ggaaagcggc cggtttcggc tcgccgcata caccgccggc
      181 gtccccgagg acttccgccg catcctaaac cggctggtct ttcagccgga tcggggcacc
      241 atcgtcggac gcgttctgat ggaacgtggt ccggtgcata tcgtggacat cgtggccgat
      301 gccgagtacc gcctcaagga ggcgcaggag gcatccgggt tccgcacagt gctgggtgtt
      361 ccactcgtgc gtgacggcgc cccgattggc gtactcgtcg tctggcggct ccaggtccag
      421 ccattcacgg atccggagat tgcgctcctg acgacctttg ccgaccaggc ggcgctggcc
      481 atcgccagcg tcaccctgtt cgagaccatt gaccgccagc gcacggagct ggcccgatat
      541 gccccgcaag ccgctgagct gttgtcgagc gtcgagggcg agcagctgct ggccggtcac
      601 cggcgcgaga tcaccgcgct ctttgccgac ctgcgcggct tcacggcctt cgctgagcag
      661 gcagagcccg aggaggtgct cggcgttctg cgccaatacc acgaagccgt cggcgaactg
      721 gcggtcgcga atggcggaac ggtggagcat tttgcaggcg atgggctgat ggtcttcttc
      781 aacgacccgc agccagtcgc cgatcaccag ctcgcggcgg tacgcaccgc ctgcgacatg
      841 cgcgagcgat tcggcgtcct gtccagtgcc tggcgcaagc gcggctacga gctcggcctt
      901 ggcatcggga tcgcgtccgg attcgcgacc ctcgggcgca tcggcttcga ggggagatac
      961 gactacgccg cgatagggaa ctcggtgatc ctcgcctcgc gactgtcaga cgcggcgagt
     1021 gccgggcaga tcctgatcag ccagcgggtc ctcgccgtga tcgaggaagc ggtagttgcg
     1081 gaaccggtcg aagacctgac gctgaagggc ttcagccacg ctctgaaggc attcgccgtc
     1141 gagagcatgc gcgtacccgc tgcctagcgg accgcggcga cgatctccgg cgccagcccg
     1201 gagtagccct ccaggaattc gatgcgctcc cgcttgagcc gctcgaccca cctgcggtac
     1261 tcgtccgccc gcccggtgcg ctcgtacagc aggcgcggct ggagcagctc caggtcatcg
     1321 acgccgggaa ggaccgaggc gacgaggtag ccgaagtcgg ggtcgcgctc ccactcgatc
     1381 gtcccctcgg cgatcgcctt gacgatcgcg ccggagtgct cgatggccac cttcttggac
     1441 gacggattgt cctcgacgcc ccccacccgg cccgtgttca gcaggaagac gtcgaagtcg
     1501 ctgctcgcca tgagttccag gaaccgattc gcctgctgct cgtcgcggta ggggtagaaa
     1561 ggattggtgc ccggcacgcg cagtgcgcga ccctcttcgg ccgctccgcc ggccgacgtg
     1621 ccctgcgtct cgccgagcat gaagtaggcc gccgcctgca ctcgcgacag gcgcgccaca
     1681 gccgggatga cgttgtcgtt gcggttcagg atcaggaggt ggttgaccgg gccgatctcg
     1741 cgcggatcac gccagatccc cagcgacgcc atggggaagg tcgctcgccc gttcttggtg
     1801 tacgaggtgt cgaagaagtc gaccggtcca tcgggctcct tctgggagac gttctccagg
     1861 taggcgtcgg gcttgacgac cgcgccgtgg at
//
LOCUS       DATCQR010000203         7275 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_27007, whole genome shotgun sequence.
ACCESSION   DATCQR010000203 DATCQR010000000
VERSION     DATCQR010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 7275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7275
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27007"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            101..1042
                     /gene="cofD"
                     /locus_tag="VIN32_06780"
     CDS             101..1042
                     /gene="cofD"
                     /locus_tag="VIN32_06780"
                     /EC_number="2.7.8.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HEY8922318.1"
                     /translation="MKVTLLAGGTGGAKLAHGFQLVLPPGDLTVIGNVADDAEPQGLL
                     VCPDLDTIMYTLAGIADRKQGWGVAGDTTTAMAQLERYGAQTWFRIGDADLATHVQRA
                     QLMAGGASLTDATATMATALGVPSHLVPATDDRLRTRLTTDAGELEFQAYFVQRGQRD
                     EVRAVRFEGADAARPSPAALVALATAELVVIGPSNPIVSIGPILAVPGMREALLASPA
                     PRVGVSGIVRGKALRGPADRMLTSLGHESSALGVARLYEGLLDRFVIDEQDEALAPQI
                     VALGMEVSVLPTVMRSDDDRASLARALLELGEGMLRG"
     gene            1039..1635
                     /gene="cofC"
                     /locus_tag="VIN32_06785"
     CDS             1039..1635
                     /gene="cofC"
                     /locus_tag="VIN32_06785"
                     /EC_number="2.7.7.68"
                     /inference="COORDINATES: protein motif:HMM:NF014085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043814 - phospholactate
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HEY8922319.1"
                     /translation="MTVRIIVPHRGLAAAKTRLAPVLDDGEREALARRLLEQVLRVAH
                     EACGDVVVITPSEALQPLVIAAGARLVVQRGMGLNAGLEQARREAIEEGITTLLILHG
                     DLPNLVADDVAALLDAIPGTPGVTIAPDRAGSGTNGLALRPPDAIGLRFGVGSFAAHL
                     AEAEVAGVPLAEVHRPGLAFDLDTPADLARWIELGDAA"
     gene            1632..2381
                     /gene="cofE"
                     /locus_tag="VIN32_06790"
     CDS             1632..2381
                     /gene="cofE"
                     /locus_tag="VIN32_06790"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012615676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HEY8922320.1"
                     /translation="MISLVAVDGIPEVKPGDDLAGMIAAALAAAGIGLEAVDVLVVTQ
                     KVVSKAEGRVVELASITPRPEAESWAAQWGKDARQVELVLQESAEVLRMAPGGLIISR
                     TRHGFVCANAGVDLSNVGGGDVATLLPEDPDASARTLRKRLGELAGASPAVVISDSFG
                     RPWRNGIVNVAIGSAGLEPLLDLRGTPDAQGRVMHSTVVAVADELASAADLAGGKVAQ
                     RPVVVVRGYEYQPGQGAASDLVMERELDLFP"
     gene            2483..3760
                     /locus_tag="VIN32_06795"
     CDS             2483..3760
                     /locus_tag="VIN32_06795"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY8922321.1"
                     /translation="MTETSLPARIDGRYLLEERIARGGSADVWRAHDEELDRPVAVKL
                     LHPHLVPDERARLRLAAEGRLVASLDHPAIVKMYDVLTEVDPPALIMELIEGESLNLK
                     LARDGALPPRAAATIGAEVAEALAEAHRHGIVHRDVKPSNILLDTAGHAHLADFGIAH
                     SLEQGAERLTQTGMVVGTPAYIAPEQLAGTEVGPRTDIFGLGSVLFEMLAGVPPFAAM
                     APLPLAQAHAAGPPPMPGIDPELVALTRACLSNSPSQRPADAGLVAATLRSYASMDPA
                     GRDAETRAIPVVAAPPAAATVPPSAPTLEERLASWWRRGMPFAAGAAALFVAVLLAAA
                     IFGPGAPAAGAPQLPSSTPTPDWVAKLMADYADACGGTLDPAVIDGLTQPDAEAQVSG
                     LIDACGNAASAPGGGGGGKGGGGSGKGKGNGKP"
     gene            complement(3786..4121)
                     /locus_tag="VIN32_06800"
     CDS             complement(3786..4121)
                     /locus_tag="VIN32_06800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8922322.1"
                     /translation="MGATETVDLFFELINDDQREDAVALFAEHAVLGISVPGSDERTN
                     LRGRDKVGGWFIRAGDGFRMYANDGRSMGASYIADVTVMRPGADSMHVEANFRVEAAH
                     IVSLFLQPI"
     gene            4276..5910
                     /locus_tag="VIN32_06805"
     CDS             4276..5910
                     /locus_tag="VIN32_06805"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEY8922323.1"
                     /translation="MSVFSDLAAEFLAEYHAEHPVQASALGLAAYDDLLDDLSADAFE
                     RRRRSDDEWLTRFGAADQSDLTFDEAIDRDLLVASLTERAIYDEWEDWRRNPDAYMNP
                     GLDGVFILFLHRLRPEAELVRSAVGRLRGIPDQLAAGRANLRPELVPALLLKRAVNQA
                     RAGARYTRELVLTQVEAANRPELAAAGAVAGDALEAYATFLEEMEPNATGDWAFGEDR
                     YNGILQRAELFDFDARALRERARGQIEEISADLKRGAQELAGHEDWHTVLLELNKDRP
                     LTPEAMRVGYEEWTAKARAFLVERGLVSFPPGEECIVEPSPPFQRPVLAVASYQDPPP
                     FSSSMRGHFFVPFPPDEASEAEIAKRLESNSYPSIPSTAVHEAYPGHHWHLVMAKLNP
                     SAVRQVFGTSYFAEGWALYAERMMRENGFYDDLRHIMFQYEAALFRAARIVVDTSLHL
                     GEMTHEEAVSFMLEHSSMTEPTAIAEVTRYCSWPTQASSYLTGCLEILRIRDRFLEAR
                     GSSLTDVDALRDFHDAITGAGTLPIALAERAVMATA"
     gene            5921..>7275
                     /locus_tag="VIN32_06810"
     CDS             5921..>7275
                     /locus_tag="VIN32_06810"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEY8922324.1"
                     /translation="MTAELDRLAEEFWDAVLAADPAQGTAIGEHRYGDRLSDISPAGR
                     AALAQRFEEIQNAVASLPPESDPEAALTQAALSQAVGDRLAILAADPHAHTVDAMSGP
                     QSSFMSVPSYQPLRNVADGESMLARWWAMGPWIDQQIAQLRRGSADGRVPVAGSVRRV
                     ITQLDELLARPLEDWPLLSPLQAGHDGWSDGAWRAFEVGLAGAVREGMRPAFARYRAY
                     LADEVLAGTRNEAHVGILHLPGGAETYASLARAHTTTDLTPQEIHDTGLAETARIDAE
                     IAELGGRVLGTPDLAGALAALRGDASVHFKSAEQIVAVAEASLARAIEAIPAWFGRLP
                     RAACEVVRMEPHEEQHSTIAYYREPPDDGSRPGRYYVNTYAPETRPRYEAEALAFHEA
                     VPGHHLQVAIGQELEQLPAFRRHAETTAFVEGWGLYSERLADEMGLYSGDLDRIGMLS
                     Y"
BASE COUNT         1065 a         2547 c         2609 g         1054 t
ORIGIN      
        1 ccaccacgct gagtggtacg gagcaccaaa aggcagtctc gagcgccgtt gcaccaccat
       61 acgtggtttc cggggctagc aggcaggcta ccctccccgc atgaaggtca cgctcctggc
      121 tggcggaacc ggcggcgcca agctggcgca tgggttccag ctggtgctcc cgcccggcga
      181 cctgacggtg atcgggaacg tggcggacga tgcggagccg caggggctcc tcgtctgccc
      241 ggacctcgac acgatcatgt acaccctcgc cggcatcgcc gaccgcaagc aggggtgggg
      301 cgtggcgggg gacacgacca ccgccatggc gcagctggag cggtacgggg cccagacctg
      361 gttccggatc ggggacgccg acctcgcgac ccacgtgcag cgtgcgcagt tgatggcggg
      421 cggagcctcc ctgaccgatg ccaccgcgac catggccact gcgctgggcg tgccgagcca
      481 cctggtcccc gccaccgacg atcggctgcg aacccgcctg acaaccgatg cgggggagct
      541 cgaattccag gcctacttcg tgcaacgcgg ccagcgcgac gaagtgcgcg ccgtgcgctt
      601 cgagggtgcc gatgccgccc gaccgagccc cgccgcgctg gtggcgctgg cgaccgctga
      661 gctggtggtc atcggtccgt ccaacccgat cgtcagcatc ggtccgatcc tggcggtccc
      721 cggcatgcgt gaggcgcttc tcgcgtcgcc cgccccgcgg gtcggcgtca gcgggatcgt
      781 gcgcggcaag gccttgcgcg gaccggccga ccggatgctc acctcgctcg gtcacgagtc
      841 gagcgcgctg ggagtggccc gcctttacga ggggctgctg gaccgattcg tgatcgatga
      901 gcaggacgag gcgctggcgc cgcagatcgt ggcgctcgga atggaggtca gcgtcctgcc
      961 cacggtcatg cgcagcgacg acgatcgggc cagcctggcg cgggcgctgc tggagctggg
     1021 cgagggtatg ctccgcgggt gaccgtccgg atcatcgtgc cgcaccgtgg ccttgccgcg
     1081 gccaagacac gcctggcccc ggtcctggat gacggtgagc gagaggcgct cgcccggcgg
     1141 ctgctcgagc aggtccttcg cgtcgcgcat gaagcctgtg gtgacgtggt ggtgatcacg
     1201 ccttccgagg cgctccaacc gctggtcatc gccgccggcg cgaggctggt ggtgcagcgc
     1261 gggatggggc tgaacgcagg cctggagcag gcgcgcaggg aggcgatcga agagggcatc
     1321 acgacgctcc tgatcctgca cggcgacctc cccaacctgg tggccgatga cgtggctgcc
     1381 ctgctggatg cgattccggg gaccccgggc gtgacgatcg ccccggaccg ggcggggtcc
     1441 ggcacgaatg ggctggcgct ccggccgccg gatgccatcg gcctgcggtt cggagtcggc
     1501 tcgtttgctg ctcaccttgc ggaggcggag gtggcaggcg tcccgctcgc agaggtgcat
     1561 cggccggggc tggccttcga cctggacacg cccgccgacc tggcgcggtg gatcgagctg
     1621 ggcgacgcgg cgtgatctcg ctcgtcgcgg tcgacggcat cccggaggtc aagccgggcg
     1681 acgacctggc ggggatgatc gcggcggcgc tcgccgccgc aggcatcggg ctggaggctg
     1741 tagacgtcct ggtcgtgacg cagaaggtcg tcagcaaggc agagggtcgc gtggtcgagc
     1801 tggcctcgat caccccgcgg ccggaagcgg agtcgtgggc ggcgcagtgg gggaaggacg
     1861 cgcggcaggt cgagctggtc ctgcaggaga gcgccgaggt gctccgcatg gcgcccggtg
     1921 ggctcatcat ctcccgtacg cggcacgggt tcgtgtgcgc caacgcggga gtcgacctct
     1981 ccaacgtcgg tggcggagat gtcgcgacgc tgcttcccga ggaccccgat gcctccgccc
     2041 gcacacttcg caagcgactg ggcgagctgg ccggggcgag cccggcggtc gtcatcagcg
     2101 actccttcgg gcgaccgtgg cggaacggga tcgtcaacgt ggcgatcggg tccgccggat
     2161 tggagcccct gctcgacctg cgcggcactc ccgatgccca gggccgcgtg atgcactcga
     2221 cggtggtggc ggtggcggac gagcttgcgt cggcggcgga ccttgcgggc gggaaggtgg
     2281 cgcagcggcc ggtggtggtg gtgcgcggat acgagtacca gcccggccag ggcgcggcat
     2341 ccgacctcgt gatggagagg gagctggacc tcttcccgta gttggcgttg acagccggca
     2401 ccggtgttag cgcgctgcaa gattcggcgc gcgccgtggc acgcgtgaag caaccacgta
     2461 ccatcaccat tcagtgtctg agatgaccga aacgagtctg cccgctcgga tcgatggccg
     2521 ctatctgctt gaggagcgga tcgctcgggg tggcagcgcg gatgtgtggc gcgcacacga
     2581 tgaggagctg gaccgccccg tcgctgtgaa gctcctgcac ccgcacctgg tgcccgacga
     2641 gcgcgcccgg ctgcgcctcg ccgccgaggg ccgcttggtg gcatccctcg atcacccggc
     2701 gatcgtgaag atgtacgacg tcctgaccga ggtcgacccg ccggcgctga tcatggagct
     2761 gatcgagggc gagtcgctca acctgaagct ggcgcgtgat ggggcgctgc cgcctcgtgc
     2821 ggccgcgacc atcggcgccg aggtcgcgga ggcactggcc gaggcgcatc gccacgggat
     2881 cgtccaccgc gacgtgaagc cgtcgaacat cctgctcgac acagccgggc acgcgcacct
     2941 tgccgacttc gggatcgcgc actcgctgga gcagggcgcc gagcggctga cccagaccgg
     3001 catggtggtc gggacccccg cctacatagc cccagagcag ctcgccggaa ccgaggtcgg
     3061 gccgcgcacc gacatcttcg ggctcggctc cgtcctgttc gagatgctcg ccggcgtacc
     3121 gccctttgcc gcgatggcgc cgctccccct cgctcaggcg cacgccgcgg ggccgccgcc
     3181 gatgcccggc atcgaccctg agctcgtggc gctgacgcgc gcctgcctct cgaattcccc
     3241 ctcgcaacgg cccgccgacg cgggcctcgt cgccgctacc ctgcgctcct acgcctccat
     3301 ggaccccgcg gggcgcgacg ccgagacgcg ggcgatcccg gtcgttgccg cgccacctgc
     3361 cgctgcaacc gtgccacctt cagcccccac ccttgaggag aggctcgcgt cgtggtggcg
     3421 tcgcggaatg ccgttcgccg ccggtgcggc cgcgctcttc gtggcggtcc tgctggcggc
     3481 cgcgatattc gggcccgggg cgccggctgc cggcgctcca cagctgccca gctccacgcc
     3541 gaccccggac tgggtcgcca agctgatggc cgactacgcc gatgcctgcg gcggaacgct
     3601 tgaccccgcc gtgatcgacg ggctcacaca gcccgatgcc gaggcgcagg tgtcaggcct
     3661 gatcgatgca tgcggcaacg ccgcgtccgc acctggcggc gggggcggag gaaagggcgg
     3721 cgggggaagc gggaagggca aggggaacgg caagccctag ccgcagctgg atcggttggc
     3781 cgcggttata tcggttgcag gaagaggctc acgatatggg ccgcctcgac ccggaagttg
     3841 gcctcgacgt gcatcgagtc ggcgcccgga cgcatgaccg tcacatcggc gatgtagctg
     3901 gcgcccatgc tgcgcccgtc gttcgcgtac atgcggaacc cgtctcccgc gcggatgaac
     3961 cagccgccga ccttgtcgcg gcctcgcagg ttagtccgct cgtcggagcc ggggacggag
     4021 atgcccagga ccgcgtgctc ggcgaacagg gcgaccgcgt cctcgcgctg gtcgtcgttg
     4081 atcagctcga agaagaggtc aacggtctcg gtggctccca tcggccgaga aggatagccg
     4141 agggaggcgg atgccacccc aaaggcggtg atacgcttcg cccgaccggc agagcgggct
     4201 ccgcggatgc cggtccaagt tgtccaaccg atgcgcgcgg tctcgcgccg cccgcgaatt
     4261 gaggggtaca cgactttgtc cgtcttctcc gacctcgctg ccgagttcct ggccgagtac
     4321 cacgccgagc accccgtgca ggccagcgcc ctgggactcg ccgcctacga cgacctactc
     4381 gatgacttgt cagccgatgc cttcgagcgc cgccggcgct ccgacgacga gtggctcact
     4441 cgcttcggcg cagccgacca gtccgatctg accttcgacg aggcgatcga ccgcgacctc
     4501 ctcgtcgcca gcctcaccga acgggcgatc tacgacgagt gggaggactg gcggcgcaac
     4561 cccgacgcct acatgaaccc ggggctggat ggcgtcttca tcctgttcct gcatcggctc
     4621 cgtccagagg ccgagctggt gcgcagcgcc gttggtcgcc tgcgcgggat cccggatcag
     4681 ctggccgcgg gacgcgccaa cctgcgtccc gagctggtcc ccgccctgct cctgaagcgt
     4741 gccgtcaacc aggcccgcgc cggggcgcgc tacacgcggg agctggtcct gacccaggtg
     4801 gaggcagcca accgtccgga gctggcagcg gccggagcag tggccggcga tgcgctcgag
     4861 gcgtacgcca ccttcctcga ggagatggag cccaacgcaa ccggggactg ggccttcgga
     4921 gaggatcgct acaacggcat cctgcagcgg gccgagctct tcgacttcga cgcccgtgcc
     4981 cttcgcgagc gagcgcgcgg ccagatcgag gagatcagcg cagacctcaa gcgcggtgcg
     5041 caggagctcg cgggccacga ggactggcac accgtcctgc tcgagctgaa caaggaccgg
     5101 ccgctcaccc ccgaggccat gcgggtcggc tacgaggagt ggacggcgaa ggcccgcgcc
     5161 ttcctggtag agcgcggcct ggtgagcttc ccgccgggcg aggagtgcat cgtggagccg
     5221 tcgccaccgt tccagcgccc cgtcctggcg gtggcctcct accaggatcc gccccccttc
     5281 tcatcgtcca tgcgaggaca cttcttcgtc ccgtttccgc cggacgaggc gagcgaggcg
     5341 gagatcgcca aacgcctgga gtccaactcg tatccgagca tcccatccac cgcggtgcac
     5401 gaggcgtatc cggggcacca ctggcacctg gtcatggcca agctcaaccc gtcggccgtg
     5461 cggcaggtct tcgggaccag ctacttcgcg gagggctggg ccctctacgc ggagcggatg
     5521 atgcgcgaga acggcttcta cgacgacctg cgccacatca tgttccagta cgaggcggca
     5581 ctcttccgcg cggcgcggat cgtggtcgac accagcctgc acctgggcga gatgacgcac
     5641 gaggaggcgg tgagcttcat gctcgagcac tcctccatga ccgagccgac cgccatcgcc
     5701 gaggtgacgc gctactgcag ctggccgacg caggccagct cgtacctgac cggctgcctc
     5761 gagatcctgc gcatccggga ccgattcctg gaagcgcgcg gctcatccct gaccgatgtc
     5821 gacgcccttc gcgacttcca cgacgcgatc accggcgccg gcacgctgcc gatcgccctg
     5881 gcagagcgcg cggtgatggc caccgcgtga gggttccagc atgacggccg agctggatcg
     5941 gctggcggag gagttctggg acgccgtcct ggcggccgac ccggcgcagg ggacggcgat
     6001 cggggagcat cgctacgggg accggctgag cgacatctcg ccggccgggc gcgcagcgct
     6061 ggcgcagcgc ttcgaagaga tccagaatgc ggtggcgtcg ctcccgcccg agtccgaccc
     6121 ggaggcagcg ctgacacagg ccgcgctgtc gcaggcggtc ggcgatcggc tcgccatcct
     6181 ggccgccgat ccccatgcgc acaccgttga cgccatgagc ggtccgcagt cgtccttcat
     6241 gagcgtcccg tcctaccagc cgctgcgcaa cgtggccgac ggcgagtcga tgctcgcccg
     6301 ctggtgggcg atgggaccct ggatcgatca gcagatcgcc cagctgcgcc gcgggtcggc
     6361 cgacggcagg gtcccggtcg ctggatccgt gcgacgggtg atcacccagc tggacgagct
     6421 tctggcgcga ccgctcgagg actggccgct cctgtcgccc ctccaagccg ggcacgacgg
     6481 ctggagcgac ggcgcctggc gcgccttcga ggtaggcctg gccggggccg tgcgggaggg
     6541 gatgcgtccc gccttcgccc gctatcgcgc ctatctcgcc gacgaggtcc tggccggtac
     6601 ccgcaacgag gcgcacgtcg ggatcctcca tctgccgggc ggggcggaga cctacgccag
     6661 cctggcccgc gctcatacaa ccacggatct gacgccgcag gagatccacg acaccggcct
     6721 ggcggagacc gcgcgcatcg acgcggagat tgccgagctg ggcggccgcg tgctggggac
     6781 cccggacctg gccggcgcgc tggccgcgct gcgcggtgat gcatcggtcc atttcaagtc
     6841 ggccgagcag atcgtggcgg tcgccgaggc gtcgctggcc cgcgcgatcg aggcgatccc
     6901 tgcatggttc gggcgcctgc cacgggccgc ctgcgaggtg gtccgcatgg agccgcacga
     6961 ggagcagcac tcgaccatcg cctactaccg cgagccgccg gatgatggca gccggccggg
     7021 ccgctactac gtcaacacct acgcaccgga gacgcggcca cgctacgagg cggaggcgct
     7081 ggccttccac gaggcggtgc cgggacacca cctgcaggtg gcgatcggcc aggagctcga
     7141 acaactgccc gccttccggc gccatgccga gacgaccgcc ttcgtcgagg gctggggcct
     7201 gtactcggaa cgcctcgccg acgagatggg gctctacagc ggcgacctcg accgcatcgg
     7261 gatgctctcg tacga
//
LOCUS       DATCQR010000204         7439 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_104217, whole genome shotgun sequence.
ACCESSION   DATCQR010000204 DATCQR010000000
VERSION     DATCQR010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 7439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7439
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104217"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..959
                     /locus_tag="VIN32_06815"
     CDS             <1..959
                     /locus_tag="VIN32_06815"
                     /inference="COORDINATES: protein motif:HMM:NF016736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HEY8922325.1"
                     /translation="VGTRSYPVQVDIAGHLRDQLHATKAGNATGQKPELVPATGVITF
                     FNRSIGSVEIPEGTQVSVGGTIAFTTTKKVNVPGGAFFGDPGQKSVAVVAVEGGTSGN
                     VAAGAIDTIDDPNLRFNRRVTNADPTGGGSEIPHSVIQQSDIDAVVAAIQTDLRQQLS
                     DALVADPDRLYAGPPTSEVPTIQIPDGLLGKEDTPTFELSGTLAYDRPYASRADAEAA
                     ARSALLADPDAAPPGTAIVGDSITLDLGEPVVVGEQLKVDASVSASAAAEIDQAKVRD
                     RIAGLTVPEAKAELAPLGKVEIDLWPGWVDRLPRLTFRIDIR"
     gene            956..1384
                     /gene="ruvX"
                     /locus_tag="VIN32_06820"
     CDS             956..1384
                     /gene="ruvX"
                     /locus_tag="VIN32_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013458446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEY8922326.1"
                     /translation="MSAVIGLDHGSKRIGVAVANEETGLAFPRPALLRRKVKADIEQV
                     AALAHAEGATRIVVGLPRNMDGSEGPQAAQARAFGEQLTAIGLEVVFVDERLTSWQAG
                     EQLAAAGRRPERTSGEKDSAAACLILQEWLDRRRPDDPTA"
     gene            1368..2696
                     /gene="mltG"
                     /locus_tag="VIN32_06825"
     CDS             1368..2696
                     /gene="mltG"
                     /locus_tag="VIN32_06825"
                     /inference="COORDINATES: protein motif:HMM:NF014656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEY8922327.1"
                     /translation="MTRQPDPRWNKNEQRNARIRELRDQREGPVRRRRTFQPLTLAAW
                     FAGVIALLGVLIFVGLLALSPRLFAWVEQHPGSIEHGVVRDFVEWYRPNALADLPAAE
                     SGKRITVEVVAGTNDAEIGQLLFDQGIISSQLAFQSAVLQLGRSGNLQAGVYDLSPSL
                     KPSEIVAALKQERGVEVTIKLTEGWRLEQVVGYLGTTKLTMNLNDFAVLAKAPPADLI
                     AEFDFLADLPKGRSLEGYLYPDTYRIDANSTARQVLEKLLGTFGERLTKKIRDGIAAQ
                     GLTIDEAVNLASIVEREAVLDAERPLIAGVYLNRINNPSAGTVGLLNADPTLQYGLAT
                     AKFGSKPIFEWQGIEWWAPLKTQGGDVPIPKELFGYQSYLVKGLPPSPIASPRIASIA
                     GVAAPDTAKGYYYFVAACPGGVRDGSHYFAKNLVGQEANIAKANAECPGQ"
     gene            2710..3270
                     /gene="efp"
                     /locus_tag="VIN32_06830"
     CDS             2710..3270
                     /gene="efp"
                     /locus_tag="VIN32_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEY8922328.1"
                     /translation="MIDTGDIKKGVIIELDGQLMKVLDWTHIKMARGSAQVRMKLQNV
                     RRGDIVERTFQAGTRWPRARVEQRKVQYLYGDGDAFHFMDNETYDQFAISAKQIGDDS
                     RFLKEGTEVFVSMYDAEVLGVDLPVAVDLRVIQTDPGFAGDTATGAKKSATLETGLVA
                     QVPLFVNEGDLLRIDTRTGDYMTRLQ"
     gene            3275..4537
                     /gene="xseA"
                     /locus_tag="VIN32_06835"
     CDS             3275..4537
                     /gene="xseA"
                     /locus_tag="VIN32_06835"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEY8922329.1"
                     /translation="MTLDSDQATLWDAPRPAPRILRVSDLNHRVRVLLDGDPALADVW
                     VEGEVSQPSYPPSGHCFFVLKDAASQVKAVLFREELARATVRPEHGMHVICHGKVRAY
                     EPQGSYQVYVESITPAGAGDLHQQYEALRTKLATEGLFDAARKRSLPRWPRRIGVVTS
                     PMGAVWRDIGNVMRRRYPIGELILSPTIVQGPTASGAIVRALHRLYTDPAIDLVILAR
                     GGGSLEDLWSFNDERVVRAVIEAPMPVVVGVGHESDVTLADFAADLRAPTPSAAAEQA
                     VPDLAQFPAILDRLRDRASAAMIGGLADRGAFLGQEGRALTRLLPDTAAARQRAAELV
                     DRGHRALSARAAREQAALDGVADALRALSPTATLERGYAVARLADGTIVRDPAQATVG
                     EPLEVVVARGTIATRVETTAGSDEEVLA"
     gene            4534..4791
                     /gene="xseB"
                     /locus_tag="VIN32_06840"
     CDS             4534..4791
                     /gene="xseB"
                     /locus_tag="VIN32_06840"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEY8922330.1"
                     /translation="MSEPEAPADLAARPFDELVAELQRIVQALEAGNLPLEESIARYR
                     EALRLHAAAELRLREAELTISELGRGMGAAPDETAPTDEEG"
     gene            4903..6873
                     /gene="dxs"
                     /locus_tag="VIN32_06845"
     CDS             4903..6873
                     /gene="dxs"
                     /locus_tag="VIN32_06845"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF003933.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEY8922331.1"
                     /translation="MPILDTLNDPAQLRGLSHAELDALAGELREKMIRTVQQTGGHLA
                     SSLGAVELTLALHRVMSSPDDRIVWDTGHQAYAHKLLTGRRDRFHTLRQLNGVGGFPR
                     RSESDHDVFDGGHAGTGVSIAVGLAAARDLLPDGDPARQQRIAVCVGDAAMQSGLTLE
                     ALNHLGDERHRLLIVLNDNEMSISPSVGGLSTRLNKLRLTRAYQETKSVTQRVLPRIP
                     IVGRLLFNALAFTKEGFKRSWAKVGFFEDMGLTYIGVLDGHNLAELEQSFERAFALPG
                     PVLVHVKTIKGKGYGPAEMDSISFHGASLPPIDLCLIDPTEEPLPPEGEPGRRPKTYT
                     ATFAEELVRLATDDDRICAITAGMPTGTGLSRFGARFPSRLFDVGIAEQHAVTMAAGL
                     ALGGKRPVVALYSTFSQRAYDQLVHDICQNDLPVLLAIDRAGLVGEDGTSHQGMFTLS
                     AQRALPNLVIGSPKDEQELRDMVVTAFAHAGPISLHYPRDPGEDLPDRDGSALEIGRG
                     EVLRDGGDLLLVGFGPIVQRLLRVAETLEKDHGMATAVINARWAKPLDEDLIAAHAVG
                     RRLVVTAEESAAMGGFGDGVLDGLNRAGIHVPLLKIALAEGFVHHGAVEDLRHQQRID
                     VPGILGQIREALALEAAPTASTSGEPSSASAA"
     gene            6879..>7439
                     /locus_tag="VIN32_06850"
     CDS             6879..>7439
                     /locus_tag="VIN32_06850"
                     /inference="COORDINATES: protein motif:HMM:NF013856.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEY8922332.1"
                     /translation="MTRVRLDQLLVDRALAPSRAAAQALLLAGEVELAGAGSRTLKPG
                     QLVDREAQLSVVARRRWASRAGEKLEAALDAFEVDPTGLACLDAGASTGGFTDVLLAR
                     AARIVYAVDVGRGQLLDRLARDPRVVSMERTNLRALRHLPEPIDLATLDLSFISLSRV
                     LPVVGVLLRAEGQVIALVKPQFEAGRD"
BASE COUNT         1198 a         2600 c         2561 g         1080 t
ORIGIN      
        1 cagtgggcac gcgctcctac ccggtacagg tcgacattgc ggggcacttg cgcgaccagc
       61 tgcacgccac caaggctggc aacgccaccg gccagaagcc cgagctggtg cctgccaccg
      121 gcgtgatcac cttcttcaac cgcagcatcg ggtcggtcga gattccagag ggcacgcagg
      181 tttcggttgg cggcacgatc gctttcacca cgaccaagaa ggtcaacgtc cctgggggag
      241 cgttcttcgg agatccaggc cagaagagcg ttgcagttgt tgcggtcgag ggcggcacca
      301 gcgggaacgt ggccgccggg gcgatcgaca ccatcgatga tcccaacctc cgcttcaacc
      361 gtcgcgtcac gaacgccgat ccaaccggcg gcggctcgga gattccgcat tcggtcatcc
      421 aacagtcgga cattgacgcg gtggtggctg cgatccagac agacctgcgg cagcagctga
      481 gcgacgcgct ggtggcagat cctgatcggc tctacgcggg accccccacc tcggaggtgc
      541 cgaccatcca gataccggac ggcctcttgg ggaaggagga cacgccgacg ttcgaactct
      601 cggggaccct tgcctacgat cgtccgtatg cgtcgcgagc ggacgccgag gcagccgctc
      661 gctccgcgct cctggccgac cccgatgcgg ccccgccagg caccgcgatc gtgggcgact
      721 cgatcacgct ggacctgggg gagcccgtgg tggtcggcga gcagctcaaa gtcgacgcat
      781 cggtgagcgc atcggcggct gccgagatcg atcaggccaa ggtgcgcgac cgcatcgccg
      841 ggctgaccgt ccccgaggcc aaggctgagc tcgccccact cggcaaggtc gagattgacc
      901 tctggccggg ctgggtcgat cgactcccgc ggctgacatt ccggatcgac atcaggtgag
      961 cgccgtgatc gggctggacc acggcagcaa gcggatcggc gtggcagttg cgaacgagga
     1021 gaccggcctc gcctttcccc gtccggcact gctgcgccgc aaggtcaagg cagacatcga
     1081 gcaggttgca gcgctggcgc atgccgaagg cgcgacgcgg atcgtggtgg gcctgccgcg
     1141 gaatatggac ggcagcgagg ggccgcaggc ggctcaggcg cgagcgttcg gggagcagct
     1201 gaccgccatc ggcctcgagg tcgtcttcgt cgacgagcgg ctgaccagct ggcaggcggg
     1261 ggagcagctg gccgctgcgg ggcgccggcc ggagaggacg tcgggcgaaa aggattcggc
     1321 ggcggcctgc ctcatcctgc aagaatggct cgaccgcagg agaccagatg acccgacagc
     1381 ctgatccgcg ctggaacaag aacgagcagc gcaacgcccg gatccgggaa ttgcgcgacc
     1441 agcgtgaggg accggtgcgc cgtcgtcgca ccttccagcc attgaccctg gcggcctggt
     1501 ttgcgggtgt catcgcgctg ctcggcgtgc tcatcttcgt cgggctgctg gcgctgtcgc
     1561 cgcgcctctt cgcgtgggtg gagcagcatc caggatcgat cgagcacggc gtggtgcgcg
     1621 acttcgtgga gtggtaccgc cccaacgccc tggcggactt gcccgctgcc gagtccggga
     1681 aacggatcac ggtcgaggtg gtggcgggga caaacgacgc cgagatcggc cagctcctgt
     1741 tcgaccaggg catcatcagc agccagctcg cattccagtc cgccgtgctc cagttggggc
     1801 gctccggcaa cctgcaggcc ggggtctacg acctctcgcc gtccctgaaa ccctcggaga
     1861 tcgtggctgc cttgaagcag gagcggggcg tcgaggtcac gataaagctc acagaaggct
     1921 ggcgcctgga gcaggtcgtc ggctacctgg gcacgaccaa gctgaccatg aacctcaacg
     1981 acttcgcggt cctcgccaag gcgccgccgg ccgacctgat cgccgaattc gacttccttg
     2041 ccgatctgcc gaagggccgc agcctcgagg gctacctcta cccggacacc tatcggatcg
     2101 acgccaattc caccgcgcgg caggtgctcg agaagctgct cggcaccttt ggtgagcggc
     2161 tgacgaagaa gatacgggac ggcatcgccg cccaggggct gacgatcgac gaggcggtta
     2221 atctggccag catcgtggag cgcgaggcgg tcctcgatgc cgagcggcca ctcatcgccg
     2281 gcgtctacct caaccgcatc aacaacccct ccgccggcac ggtggggctt ctgaatgccg
     2341 acccgaccct ccagtacggg ctggccaccg ccaagttcgg atcgaagccg atcttcgagt
     2401 ggcaggggat cgaatggtgg gcgccgctca agacgcaggg aggcgacgtg ccgattccca
     2461 aggagctgtt cgggtaccag tcgtacctgg tcaaaggact ccccccttcg ccgattgcct
     2521 cgccgcgcat cgcctcgatc gccggcgtgg cagcgcccga cacggcaaag ggctactact
     2581 acttcgtggc agcctgcccc ggcggcgtgc gcgacggatc gcactacttc gccaagaacc
     2641 tggtcgggca ggaggccaac atcgcgaagg ccaatgccga gtgccccggg cagtgacgga
     2701 gagaacggca tgatcgatac cggcgacatc aagaagggcg tcatcatcga gctggacggg
     2761 cagttgatga aggtcctgga ctggacgcac atcaagatgg cgcgtggatc ggcgcaggtc
     2821 cgcatgaagc tccagaacgt ccgacgcggc gacatcgtgg agcgcacctt ccaggccggg
     2881 acgcgctggc cccgagcgcg ggtggagcag cgcaaggttc agtacctgta cggcgacggc
     2941 gacgcgttcc acttcatgga caacgagacc tacgaccagt tcgccatcag cgcaaagcag
     3001 atcggcgacg actcccgctt cctgaaggag gggaccgagg tcttcgtgag catgtacgac
     3061 gccgaggtgc tgggggtcga cctgccggtc gcggtcgacc tgcgcgtgat ccagaccgat
     3121 ccgggcttcg ccggcgacac cgccaccggc gccaagaagt cagccacgct cgagaccggc
     3181 ctggtggcgc aggtgccgct cttcgtcaac gagggcgacc tgctgcgcat cgacaccagg
     3241 accggcgact acatgacccg cctgcaatag gccgatgacg ctcgattccg accaggccac
     3301 gctctgggac gcgccgcgcc cggcgccgcg catcctccgc gtctcggacc tgaaccaccg
     3361 ggtgcgcgtc ctgctcgacg gcgatcccgc gctggccgac gtgtgggtcg agggcgaggt
     3421 cagccagccc tcatacccgc catcgggtca ttgcttcttc gtcctgaagg acgccgccag
     3481 ccaggtcaag gcggtcctgt tccgcgagga gcttgcccgc gccaccgtcc gtcccgagca
     3541 cggcatgcac gtgatctgcc acggcaaggt gcgcgcatat gagccgcagg gcagctacca
     3601 ggtctacgtc gagtcgatca cgccggccgg cgcgggggac ctgcaccagc agtacgaggc
     3661 gctgcgcacc aagctggcca cggaggggct cttcgacgcg gcacgcaagc gctccctgcc
     3721 gcgctggccg cggcggatcg gggtggtcac ctcgccgatg ggggccgtgt ggcgcgacat
     3781 cggcaacgtc atgcgccggc gctacccgat cggggagctg atcctgtccc cgacgatcgt
     3841 ccagggaccg accgcctcgg gcgcgatcgt acgggcgctg catcgcctct acacggaccc
     3901 ggcgatcgac ctcgtcatcc tggcccgcgg cggcggctcg ctcgaggacc tgtggtcgtt
     3961 caacgacgag cgcgtggtgc gcgccgtgat cgaggcgccg atgccggtag tggtcggcgt
     4021 cgggcacgag tcggacgtga ccctagccga cttcgccgcc gacctccgcg caccgacccc
     4081 atcggcagcg gccgagcagg cggtccctga cctcgcccag ttcccggcca tcctcgatcg
     4141 ccttcgggat cgcgcctcgg cggcgatgat cggcggcctc gccgatcgcg gcgccttcct
     4201 gggccaggag gggcgtgccc tgacccgcct gctgcccgat accgcggcgg cccgtcagcg
     4261 cgccgcggag ctcgtcgatc gcggccatcg cgcgctgagc gcccgcgccg cccgcgagca
     4321 ggctgcgctg gatggcgtgg ccgacgccct gcgtgccctg tcgccgactg ccaccctgga
     4381 gcgtggctat gccgtggcac ggctcgccga cggcacgatc gtgcgcgacc ccgcccaagc
     4441 caccgttggc gagccgctgg aggtggtggt ggcacgcggt acgatcgcca cccgcgtgga
     4501 gacgacggcc gggtcggacg aggaggtgct ggcatgagcg aacccgaggc gcccgccgac
     4561 ctcgcggcgc gacccttcga cgagctcgtc gccgagctgc agcgcatcgt ccaggcgctg
     4621 gaggcgggca acctgcccct cgaggagagc atcgcccgct accgagaggc gctgcgcctc
     4681 cacgccgctg ccgagctgcg gctgcgcgag gccgagctga ccatcagcga gctcgggcgc
     4741 ggaatggggg cggcgcccga cgagacagcc cccaccgacg aggagggcta gccgggtgga
     4801 tatgaagtct ccaagagctc gtacttcctg acccaggcgc tcgactgacc tgacccctgc
     4861 cctgacctat actttcgcgc gccaccgcca cgcggagaca ccttgccgat cctcgacaca
     4921 ctgaacgacc ccgcacagct gcgcgggctg agccacgcgg agctggacgc gctcgccggc
     4981 gagctccgcg agaagatgat ccggaccgtc cagcagaccg gcggccacct ggccagctcg
     5041 ctcggtgcgg tcgagctcac ccttgccctg caccgtgtga tgagctcgcc cgatgaccgc
     5101 atcgtatggg acaccggaca ccaggcctat gcccacaagc tgctgacggg acgccgcgat
     5161 cggttccaca cccttcgcca gctgaacggg gtcggcggct ttccgcggcg cagcgagagc
     5221 gaccacgacg tcttcgacgg gggccacgcc gggacggggg tcagcatcgc ggtcgggctg
     5281 gccgcggcgc gcgacctgct gcccgacggc gatccggcgc ggcagcagcg catcgccgtc
     5341 tgcgtcgggg acgcggcgat gcagagcggc ctcacgcttg aggcgctcaa ccacctcggc
     5401 gacgagcggc atcgcctcct gatcgtgctc aacgacaacg agatgagcat cagcccctcg
     5461 gtcggcggcc tctcaacacg cctcaacaag ctgcgcctga cccgtgccta ccaggagacg
     5521 aagtcggtga cgcagcgggt cctgccccgc atcccgatcg tcggccgcct cctgttcaat
     5581 gcgctggcct tcaccaagga gggcttcaag cgctcgtggg ccaaggtcgg cttcttcgag
     5641 gacatggggc tcacctacat cggcgtcctc gacggccaca acctggccga gctggagcag
     5701 agcttcgagc gcgccttcgc cctgcccggg ccggtgctgg tccacgtcaa gacgatcaag
     5761 ggcaagggct atggcccggc cgagatggac agcatcagct tccatggagc cagcctgccg
     5821 ccgatcgacc tgtgcctgat cgacccgacc gaggagccgc tgccgcccga gggcgagccg
     5881 gggcgacgcc cgaagaccta caccgccacc ttcgccgagg agctggtgcg gctggcgacc
     5941 gatgacgatc ggatctgtgc catcactgcg gggatgccga ccgggacggg gctgtcgcgc
     6001 ttcggtgcgc gcttcccgtc gcgcctcttc gacgtcggga tcgccgagca gcatgccgtc
     6061 actatggccg cgggcctggc gctgggtggc aagcgcccgg tggtcgcgct ctactcgacc
     6121 ttcagccagc gggcgtatga ccagctggtg cacgacatct gccagaacga cctgccggtg
     6181 ctgctggcga tcgaccgcgc cggcctcgtc ggcgaggacg gcaccagcca tcagggcatg
     6241 ttcaccctct ccgcgcaacg cgccctgccg aacctggtca tcggctcacc gaaggacgag
     6301 caggagctgc gggacatggt ggtgacggca tttgcacacg caggaccgat cagcctgcac
     6361 tatccccgag accccggcga agacctcccg gaccgtgatg gatcggccct cgagatcggc
     6421 cgtggcgagg tgctgcgcga tggcggcgac ctgctgctgg tcggcttcgg accgatcgtg
     6481 cagcggctgc tgcgggtcgc cgagacgctg gagaaggacc acggcatggc gacggccgtc
     6541 atcaatgcac gctgggcgaa gccgctcgac gaggacctga tcgcagcgca cgcggtgggc
     6601 cgccgcctgg tcgtgaccgc tgaggagagc gccgccatgg gcgggttcgg cgacggcgtg
     6661 ctcgacgggc tcaaccgggc cgggatccat gtgccgctcc tcaagatcgc cctggccgag
     6721 ggattcgtgc atcacggtgc cgtcgaggac ctgcggcacc agcagcggat cgacgtgccg
     6781 ggaatcctgg gccagatccg ggaggcgctc gccctcgagg cagctcccac cgcgtcgacc
     6841 tccggcgagc cgtcgagcgc aagcgccgcc tgaccggcgt gacccgggtc cgcctcgacc
     6901 agctcctcgt cgatcgcgcg ctggcaccga gccgcgccgc agcccaggct ctcctccttg
     6961 ccggcgaggt cgagctggcc ggtgccggca gtcgcaccct caagcccggc cagctggtcg
     7021 accgcgaggc gcagctgtcg gtggtcgcac gccgtcgctg ggcatcccgc gccggtgaga
     7081 agctggaggc ggcgctggat gcgttcgagg tcgatccaac gggtctcgcc tgcctggacg
     7141 ccggcgcatc caccggcggg ttcaccgacg tgctcctcgc ccgggccgcg cggatcgtgt
     7201 atgcggtgga cgtcggtcgt gggcagctgc tcgaccggct ggctcgcgat cctcgcgtgg
     7261 tcagcatgga gcgcaccaac ctgcgagcgc tgcgccacct gccggagccg atcgacctcg
     7321 caacgctcga tctatccttc atatcgctca gccgcgtcct tcctgtcgtg ggcgtgctgc
     7381 tgcgagccga gggacaggta atcgccctgg tcaagccaca gttcgaggcc ggccgggac
//
LOCUS       DATCQR010000205         2308 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_196943, whole genome shotgun sequence.
ACCESSION   DATCQR010000205 DATCQR010000000
VERSION     DATCQR010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2308
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196943"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..700
                     /locus_tag="VIN32_06855"
     CDS             <1..700
                     /locus_tag="VIN32_06855"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HEY8922333.1"
                     /translation="PGDYPVILEEYAVATVLEYLSWMGFSALAVEEGRSFMDLGQQIM
                     GANVSIWDDGLDPLGLPTAIDYEGVPKQRVDLIRNGVAHAVVHDTATASRAGVASTGH
                     GLPAPNAFGPMAWNLFMSPGSSAKEVMLSGIERGVWVTRFHYVNIVHAKRGILTGMTK
                     DGTFLIEDGRITRPIRNLRFTQSIPEAFSAIEAIASETRLVAAEYSGINARVPALRLA
                     KFAFTGATGAEAQG"
     gene            703..909
                     /locus_tag="VIN32_06860"
     CDS             703..909
                     /locus_tag="VIN32_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008475827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HEY8922334.1"
                     /translation="MAAVDLRIGDRIGLRKAHPCGSREWRVARIGADIGLVCEGCGHR
                     ILMDRLDVERRFTGHLERGPQAAP"
     gene            913..>2308
                     /locus_tag="VIN32_06865"
     CDS             913..>2308
                     /locus_tag="VIN32_06865"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8922335.1"
                     /translation="MAHMELPPHVELPPDLPVSEPSGSVFANRSFVLLWVAQVLSQLA
                     SNMVLAGLMATVVLATRSNTANAVLILTFLVPAVAFSAIAGVLVERSDARLIMLASNL
                     LRGGGIVLFLLVGSNVGLIFVINLLVATVTAFFVPAELTAIPRLVDRRQLMAANSTFV
                     LTINATFAIGFGFLGPLLLTTLGVNAVFIVVAVMFGLAALAIVPLPAVPPEKHAEIGV
                     KETRQAFTVVFDQLKEGIAFVRSHRPIAWSLAYLGIAASLIAVMGAIGPGFATDILLL
                     RPQDFFFVMGPAGMGAVLGILILNAYGANLPRRLLIDCGLVAMGVTLIGLALVKPITV
                     FLSPALQPIENNLPAALSPLLSLIALVVVIAITAGVEYAFVAIPAQTALQEDLPVEVR
                     GRIFGILNTLLSVASFLPVLVAPAAADLVNLLVKGAGIPVVMAILGLVTLVLGILSWR
                     HNKAGGLHRTNLALS"
BASE COUNT          323 a          804 c          758 g          423 t
ORIGIN      
        1 gcccggcgac tacccggtca tcctcgagga gtacgccgtg gcgaccgtcc tcgaatacct
       61 gtcgtggatg ggcttctcgg cactggcggt ggaggaggga cggtcgttca tggacctcgg
      121 ccagcagatc atgggcgcga acgtctcgat ctgggacgac gggctggatc cgctcggcct
      181 gccgacggcg atcgactacg agggggtccc aaagcagcgc gtcgacctga tcaggaatgg
      241 cgtcgcccac gccgtggtcc acgacaccgc gaccgcgtcg cgggctggcg tcgcgtcgac
      301 cggtcacggg ctccccgcgc caaatgcctt cggcccgatg gcctggaacc tgttcatgtc
      361 ccccgggtcc agcgccaagg aggtgatgct gtccggcatt gagcgcggcg tgtgggtgac
      421 tcgcttccat tacgtcaaca tcgtgcatgc caagcgaggc atcctcacgg ggatgaccaa
      481 ggacggcacc ttcctgatcg aggatggccg catcacccgc ccgattcgca acctgcgctt
      541 cacccagtcg atccccgagg ccttcagcgc gatcgaggcg atcgcctccg agacgcgcct
      601 tgtcgccgcg gagtactccg gcatcaacgc gcgggtgcct gcgctgcgac tggcgaagtt
      661 cgccttcacc ggggccaccg gggcggaggc ccagggctag cgatggccgc ggtcgacctg
      721 cgcatcggcg acaggatcgg gctgcgcaag gcccacccct gcggcagccg cgagtggcga
      781 gtggcgcgca tcggcgccga catcggcctg gtatgtgagg gttgtgggca tcggatcctg
      841 atggaccgcc tcgatgtcga gcgccgtttc accgggcacc tcgagcgcgg tccccaggcg
      901 gcaccctgac cgatggccca catggagctg cctccccacg ttgagctgcc gccggacctg
      961 ccggtctccg agccgtccgg ctccgtattc gcgaaccgct ccttcgttct gctgtgggtg
     1021 gcccaggtgc tcagccagct ggccagcaac atggtgcttg ccggcctcat ggccacggtc
     1081 gtgctggcca cccggtcgaa cacggccaac gccgtcctga tcctgacctt cctggtgccg
     1141 gccgtcgcct tcagcgccat cgccggggta ctcgtcgaac ggagcgacgc gcgcctgatc
     1201 atgctggcct cgaacctgct gcgcggcggt gggatcgtgc tcttcctgct ggtcggcagc
     1261 aacgtcggcc tgatcttcgt gatcaacctg ctggtcgcca cggtgaccgc cttcttcgtg
     1321 cccgccgagc tgacggcgat ccctcgactg gtcgatcggc ggcagctgat ggcggccaac
     1381 tcgaccttcg tgctgacgat caacgcgacc ttcgccatcg gctttggctt cctgggaccg
     1441 ctgctgctga cgacgctggg cgtgaacgcc gtcttcatcg tggtggcggt gatgttcggc
     1501 ctcgcggcgc tcgcgatcgt gccgctgccc gccgtcccgc cggagaagca tgcggagatc
     1561 ggagtgaagg agaccaggca ggcgttcacc gtggtcttcg accagctcaa ggaggggatt
     1621 gccttcgtgc gcagccatcg accgatcgcc tggtcgctgg cctacctcgg gatcgcagcc
     1681 agcctgatcg cggtgatggg tgccatcggg ccgggctttg cgaccgacat cctcctgctg
     1741 cgtccacagg acttcttctt tgtcatgggt ccggcgggga tgggcgccgt cctcggcatc
     1801 ctcatcctga acgcgtatgg cgccaacctc ccgcggcggc tcctgattga ctgtgggctg
     1861 gtggcgatgg gggtgacgct catcggcctt gcactggtga agccgatcac cgtcttcctg
     1921 tcgcccgccc tgcagccgat cgagaacaac ctgccggccg cactcagccc gctcctgtcg
     1981 ctgatcgcac tggtggtggt gatcgccatc acggccggtg tcgaatacgc gttcgtcgcc
     2041 attccggccc agactgccct ccaagaggac ctgccggtgg aggtccgagg ccgcatcttc
     2101 ggcatactca acaccctgct gtcggtcgca tccttcctgc cggtgctcgt cgcgccggcc
     2161 gccgccgacc tggtgaacct cctcgtcaag ggcgccggga tcccggtggt gatggcgatc
     2221 ctcggactcg tcacgctggt gctcgggatc ctctcgtggc gacacaacaa ggccggcggc
     2281 ctgcatcgca ccaatctcgc cctgtctc
//
LOCUS       DATCQR010000206         1731 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_182154, whole genome shotgun sequence.
ACCESSION   DATCQR010000206 DATCQR010000000
VERSION     DATCQR010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182154"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(109..1032)
                     /locus_tag="VIN32_06870"
     CDS             complement(109..1032)
                     /locus_tag="VIN32_06870"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY8922336.1"
                     /translation="MPNRPWLVPAAFILLGLIWGSSFLWIKIGVDEMDPATLVAYRVS
                     LGAVAMLAYARLTGERLLRTPRELVPMAALGMVATALPFFLITWGEQYIDSGTAAVLN
                     SLTPIFSLVLAGAILHTESVTLVRVIGLVTGFAGAALLASREFALRGDPLALIGVGAV
                     TVAAISYALGVSVSKAMVHGTPRNVVAAGNLTFGAIYIWIFALVSGADPALPRQGDSI
                     IAVLWLGLLGSFLAFVLLYYLISNLDATVATMVTYLFPVVGVTLGVVFLGEVLDARLL
                     IGTALVLAGIVVVSLRYDQVVGLVTGLRGRA"
     gene            1062..>1731
                     /locus_tag="VIN32_06875"
     CDS             1062..>1731
                     /locus_tag="VIN32_06875"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEY8922337.1"
                     /translation="MPTLRVAACQIDPQLGEVEGNLDRIGRAVAEAAVAGAVLAVLPE
                     AAVTGYAFGSLEEARPVARRAGVVAQDVIAGLAEVHRMTVICGTLEAQGDEVFNAALV
                     VTPDGRRYTYRKMHLPFLGVDRFTTPGPDAPSVIEVNGLRIGVLICYDLRFPEAARMC
                     ALDGADLIALPTNWPVGVDFHPGIFAPSRAAENHCYLLACDRVGTERGTTFIGRSVLI
                     DYDGK"
BASE COUNT          295 a          590 c          619 g          227 t
ORIGIN      
        1 gctgaggctc tggctcggcg ggcggctcgt tcgggtcgta ccagcgggga cgccacggct
       61 cggccggggg ctcctggtcc ggccagcgcg gcccttcgtc gaagccgctc atgcgcggcc
      121 tctcaagccg gtgaccaatc ccacgacctg atcatagcga aggctcacca ccacgatccc
      181 ggccagcacc agggcggtgc cgatcaggag gcgtgcgtcg agcacctcgc caaggaacac
      241 gacgcccagc gtgaccccca ccaccgggaa gaggtaggtg accatggtgg ccaccgtggc
      301 gtccaggttt gagatgaggt aatagaggag cacgaaggct aggaacgagc cgagcagccc
      361 cagccacagc accgcgatga tgctgtcgcc ctggcgcggg agcgccggat cggctccgct
      421 gaccagggcg aagatccaga tgtagatggc gccgaaggtc aggtttccgg cagccaccac
      481 gttgcgcggg gtcccgtgga ccatcgcctt cgacacggag accccgagcg cgtaggagat
      541 ggccgccacg gtgaccgcgc cgacgccgat cagcgccagc ggatcgccac gcagcgcgaa
      601 ctcccgggag gcgagcagtg cggcgcccgc gaagcccgtc accaggccga tcacgcgcac
      661 cagcgtcacc gactccgtat gcaggatggc ccctgccagg acgaggctga agatcggggt
      721 cagcgagttg agcaccgcgg cagtgcccga gtcgatgtac tgctcccccc aggtgatcag
      781 gaagaacggg agggcggtgg cgaccatccc cagggcggcc atcgggacca gctcgcgggg
      841 cgtccggagc agccgctcgc cggtgagtcg cgcgtaggcg agcatggcca ccgcacccag
      901 gctgacccga taggcgacca gcgtggcggg gtccatctcg tccacgccga tcttgatcca
      961 gaggaaggag gagccccaga tcagcccgag taagatgaag gcagccggga ccagccaggg
     1021 gcgattgggc atcggcgaag ggtagcggga aggaagggca gatgccgacg cttcgggtgg
     1081 ccgcctgcca gatcgatcca cagctcgggg aggtcgaggg gaatctcgac cggatcgggc
     1141 gcgccgtcgc agaggccgcc gtcgcaggag ccgtgctcgc cgtcctgccc gaggccgccg
     1201 tcaccggata cgccttcggc tcgctggaag aggcgcggcc ggttgcccga cgggcggggg
     1261 tcgtggccca ggacgtgatc gccggcctcg ccgaggtgca ccgcatgacg gtcatctgcg
     1321 gcacgctcga ggcgcagggc gacgaggtct tcaatgccgc gctggtcgtc accccggatg
     1381 gccggcgcta cacctaccgc aagatgcacc tgcccttcct tggggtggac cgattcacca
     1441 cgcccgggcc cgatgcgccg agcgtgatcg aggtcaacgg cctgcgcatc ggcgtcctga
     1501 tctgctacga cctgcgcttc ccggaggcag ctcggatgtg cgccctggac ggcgccgatc
     1561 tgatcgcgct gccgaccaac tggccggtgg gcgtcgactt ccaccccggc atcttcgccc
     1621 cgtcccgcgc ggcggagaac cactgctacc tgctggcctg cgaccgggtc gggacggagc
     1681 gcgggacgac cttcatcggt cgctcggtgc tgatcgacta cgacgggaag c
//
LOCUS       DATCQR010000207         2841 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_27232, whole genome shotgun sequence.
ACCESSION   DATCQR010000207 DATCQR010000000
VERSION     DATCQR010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2841
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27232"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..424
                     /locus_tag="VIN32_06880"
     CDS             <1..424
                     /locus_tag="VIN32_06880"
                     /inference="COORDINATES: protein motif:HMM:NF024835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY8922338.1"
                     /translation="RRRMTLQTPRSFNSWLRGQLKEKKMSQRQLALQSGVDHSTISRL
                     IKGDRMPSLGTATKLARGLREIREESDGPAYFATQGARQLQPTARVEYALRGDDLLSE
                     ADVRGLMQTYIALRGRRLRDETNGEHELAAGNGNGYRT"
     gene            complement(421..1401)
                     /locus_tag="VIN32_06885"
     CDS             complement(421..1401)
                     /locus_tag="VIN32_06885"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016888 - endodeoxyribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HEY8922339.1"
                     /translation="MAGTVARRQPRRRLTWPAGAPRVGMHLGVASGLLKAGRRARQIG
                     ATALQIFSDNPTAWRRRPEPPPDAAEFVAFCAREGIAPVAIHASYLINLAGSAEPFAR
                     QSLEGLAYEMQRAPAYGATLVNTHIGSHRGDGRARGIARLAANISAVLAGSPEGVRLV
                     LENSAGGGDTLGGTLEELAAILEAVPPGDGDQLAFCLDTAHLWGAGYDISSAEGATAV
                     LDRFDELIGLDRLALVHLNDSRSLLGSRGDRHEHIGAGKIGAAGLAAFLRDPRLPGRT
                     ALVMETPGVDEGFDAVNMRRSWLLQGGADTLPVLPPRAFRTSRASTRRSA"
     gene            1400..1522
                     /locus_tag="VIN32_06890"
     CDS             1400..1522
                     /locus_tag="VIN32_06890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8922340.1"
                     /translation="MEPEPRTPRRLDLLSIALLVFFVSLIVLVAALLLLPTLAA"
     gene            complement(1529..2110)
                     /gene="raiA"
                     /locus_tag="VIN32_06895"
     CDS             complement(1529..2110)
                     /gene="raiA"
                     /locus_tag="VIN32_06895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEY8922341.1"
                     /translation="MRTTVNSRHLELTEAMRAQIDRKLHLLERITHPDADATVELIVH
                     ASRAAETSHVAEVSLVNNGTVIRSVGTGPTMTAALDNMVDRLERQVVRSRERPRSVRE
                     RAEEGLQSAAPENLDSEADAEESPVPRVVEIKRYDMTPMFEEDAILRMDELGHAFFVF
                     LNAETDRIGVVYRRGDGSYGMIDPVVERSGRSS"
     gene            complement(2168..>2841)
                     /locus_tag="VIN32_06900"
     CDS             complement(2168..>2841)
                     /locus_tag="VIN32_06900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEY8922342.1"
                     /translation="PSAQAGRMRVLDLLLPPACAGCGRFGHLVCRDCVTGFRAASAPD
                     DRFMAADPGTTVGDALELAMAAFVHAGTLRRALQRLKYGGSGRIAVPLARAAAPALEA
                     LTLVCGPLPLVPVPVHLARRRQRGYNQAALLADALATEAGLRVCDILERRRATARQHG
                     LGKAARLHNLRGAFALRDGARTPPGVILVDDILTTSATLESCARVLRDAGAAAVYGFA
                     IAREV"
BASE COUNT          440 a          993 c          976 g          432 t
ORIGIN      
        1 acgaaggcgc atgacgctcc agactccgcg gtcgttcaac agctggctgc gaggccagct
       61 gaaggaaaag aagatgagcc agcgccagct ggccctccag tcgggtgtcg atcactcgac
      121 catctcccgc ttgatcaagg gcgaccggat gccgtcactc ggcacggcca ccaagctggc
      181 ccgaggcctg cgcgagatcc gcgaggagtc cgacggccct gcctacttcg ccacacaggg
      241 cgcccggcag ctgcagccga cggcaagggt cgaatatgcg ctccgcggcg acgatctcct
      301 ctcagaggcc gatgttcgcg gtctgatgca gacctacatc gcgctgcgag gtcggcggct
      361 ccgagacgaa acgaacggcg agcacgagct cgccgcaggc aacgggaacg gctaccgcac
      421 ctaggccgac cgtcgcgttg atgcccgaga ggtcctgaaa gcccgaggtg gaaggaccgg
      481 gagcgtgtcg gctcccccct gcaggagcca gctgcggcgc atgttgacgg cgtcgaagcc
      541 ctcgtccaca ccgggggtct ccatcacgag ggcagtgcgc ccgggaagtc gcggatccct
      601 caggaaggcg gccaggccgg ccgccccgat ctttcccgca ccgatgtgct cgtgccggtc
      661 ccctcgcgac cccaacagcg agcgcgaatc gttcaggtga accagggcca gccgatcgag
      721 cccgatcagc tcgtcgaacc ggtcgaggac tgcggtggcc ccctccgcgc tcgagatgtc
      781 gtacccggcg ccccacaggt gcgctgtgtc gaggcagaag gccagctggt cgccgtcgcc
      841 gggtggcacg gcctcgagga tggcggccag ctcctcgagc gttcctccca gcgtgtcgcc
      901 gccgccagcg gagttctcca gtaccagccg caccccctca ggcgagccgg ccagcaccgc
      961 cgagatgttg gcggccagcc gcgcgatgcc gcgcgctcgc ccgtcgccgc gatgcgatcc
     1021 gatgtgcgtg ttgaccagcg tcgcgccgta tgccggggcg cgttgcatct cgtaggccag
     1081 gccttcaagc gactgacgag cgaacggctc cgccgatcca gccaggttga tgaggtagct
     1141 ggcgtgaatc gcgaccggcg cgattccctc ccgagcgcag aaggcgacga attcggcggc
     1201 gtcgggtggc ggctcggggc ggcgccgcca cgcggtcggg ttgtcgctga agatctggag
     1261 ggcggtcgcc ccgatctgcc gtgcccgccg cccggccttg aggagcccgc tcgccactcc
     1321 aaggtgcatt ccgacccggg gcgcgccggc cggccaggtg agacggcggc gcggctggcg
     1381 tcgtgctacg gtgccggcca tggagcctga gcccagaacg ccgcgacgcc tcgacctgct
     1441 gtcgatcgcg ctgctcgtct tcttcgtctc gttgatcgtg ctggttgcag ccctgctgct
     1501 cctgccgacc ctggcggcct gaggccgctc agctcgacct cccggaccgt tcgacgaccg
     1561 gatcgatcat cccgtaggaa ccgtcgccac ggcggtagac aaccccgatg cgatcggtct
     1621 cggcattgag gaagacgaag aaggcgtggc cgagctcgtc catgcgcagg atggcgtcct
     1681 cctcgaacat cggcgtcatg tcatagcgct tgatctcgac cacgcggggg acaggcgact
     1741 cctctgcgtc tgcctcgctg tccaggttct ctggcgctgc gctctgcagc ccctcctccg
     1801 cgcgctcccg cacacttcga ggccgttcgc gcgaacgaac cacctgtcgc tcaagccgat
     1861 cgaccatgtt gtcgagcgcg gccgtcatgg tcggtcctgt gccgacggag cggatgactg
     1921 tgccgttgtt cacgaggctc acctcggcca cgtgcgaggt ctccgcggca cgagaggcgt
     1981 ggacgatgag ctcaacggtg gcatcggcat cggggtgggt gattcgctcc aggagatgca
     2041 gcttgcggtc aatctgggca cgcattgcct cggtcagctc gaggtggcgc gagttcacgg
     2101 tggtcctcat ttcgggtcct ccgctgcgag gcccctacgg tagcaccgga gccccgacga
     2161 ctccgcgcta gacctcgcgc gcgatggcga agccatagac ggccgccgcg cctgcgtcac
     2221 ggaggacccg ggcgcatgat tcgagggtcg ccgatgtggt caggatgtcg tcgaccagga
     2281 tgacgccggg cggggtccga gccccatcgc gcaatgcgaa ggcgcctcgc aggttgtgca
     2341 gtcgagctgc cttgcccagg ccgtgctggc gggccgttgc cctgcgccgt tcgaggatgt
     2401 cgcacacccg cagaccggcc tccgtggcca gcgcatccgc caggagtgcc gcctggttgt
     2461 agccgcgctg tcgccggcgg gccagatgta cgggcacggg cacgagcgga agcggtccgc
     2521 agaccagcgt cagagcctcc agggcgggcg ctgcggcccg tgcgagggga acggctattc
     2581 ggccactgcc gccgtacttg agccgttgca gggctcgtcg caacgttccg gcgtgcacaa
     2641 aggctgccat tgccaactcg agcgcatcgc cgaccgtcgt gcccgggtcg gctgccatga
     2701 accggtcgtc aggcgcagat gccgctcgaa accccgtcac gcagtcgcgg cagacgagat
     2761 gaccgaatcg gccacagccc gcgcaggccg gcggcaggag gagatccagc acgcgcatcc
     2821 ggccagcctg cgcgctcgga c
//
LOCUS       DATCQR010000208         6927 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_43045, whole genome shotgun sequence.
ACCESSION   DATCQR010000208 DATCQR010000000
VERSION     DATCQR010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6927
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43045"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /gene="ffs"
                     /locus_tag="VIN32_06905"
     ncRNA           complement(<1..181)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VIN32_06905"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            complement(228..315)
                     /locus_tag="VIN32_06910"
     tRNA            complement(228..315)
                     /locus_tag="VIN32_06910"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(279..281),aa:Ser,seq:gga)
     gene            complement(330..416)
                     /locus_tag="VIN32_06915"
     tRNA            complement(330..416)
                     /locus_tag="VIN32_06915"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(380..382),aa:Ser,seq:cga)
     gene            complement(442..885)
                     /locus_tag="VIN32_06920"
     CDS             complement(442..885)
                     /locus_tag="VIN32_06920"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: protein motif:HMM:NF025793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEY8922343.1"
                     /translation="MNYELYMAEAIAEARRGAEEGETPIGAVAVLDDAMIARDHRRIL
                     LQRDPTAHAVLLTLQAAARKLDAKRLPELTVFTTHEPCSMCVGALLEAQIRTLVYAVP
                     DEKRGAAGGAVQLARAPGMPHQISVISGVREDEARRLTASAPAPA"
     gene            1030..2481
                     /locus_tag="VIN32_06925"
     CDS             1030..2481
                     /locus_tag="VIN32_06925"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HEY8922344.1"
                     /translation="MAIVTVGAAVVRLAGLTTPGGLVFDEIFYARNACRFVIDTAQCG
                     IDDLVSGAHPPLGNWLIGIGIRLFGYDEFGWRIASAAAGTLSVALLYVLVRRLLAGHV
                     PATAATGGATAAAALLATDFLDLVQSRVAMLDSFVTMLVIAVVLCVVLDGGRRRGPRG
                     EDLGAPRWLRLLSLGRPWRLAAGLALGAAVAVKWSGGYVAFGLIALLVAWEVAASASR
                     PGDDQRPGWRAAVGDAFRREAIPSLVLLGVVPVIVYLASYTGRVDGDLLALPWRAGSV
                     WRGILEHQLAMLRFHLGLAGDHPYESAPWSWILLERPVAYSFASSSNAYREVLAIGNP
                     LTWWPGAVALVAVGVRWVRSGASLARPEVVLLVAALSTYLPWLLLSGSRSQVFIWYLL
                     PTIPFLYAALGLYTALAWRSVTGRIATGLAAVAIAISFAFFYPILTAAPLTPAEWRSH
                     IWFADCRRPGAPTLVIPDDEINRGPPPSGWCWI"
     gene            complement(2478..3824)
                     /locus_tag="VIN32_06930"
     CDS             complement(2478..3824)
                     /locus_tag="VIN32_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018008838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY8922345.1"
                     /translation="MSFSTLGLAPGLLRAVSAQGYTEPTPVQREAIPFVLAGRDLLAG
                     AQTGTGKTAAFVLPMLQLLATTPPRSGGRPRIRALILVPTRELAIQVEESVRVYGSNS
                     RRSTTIYGGVGFPGQLRALRNGPEIVVATPGRLLDHSRQRTIDLGAVEILVLDEADRM
                     LDMGFIRDIRSIIALLPPRRQNLLFSATFSNEIRALAEGLLDRPASVQVTPRNTPTDL
                     VRQVVHPVDRARKRELLSHLVRTHAVDQALVFTRTKHGADRLAKQLLEDGIATAAIHG
                     NKSQPQRVRALNDFKAGRVTLLIATEVAARGLDIEQLPHVVNYELPMVPSDYLHRIGR
                     TGRAGLEGDAISLVCIDEAPMLREIERLLGRPIPSEVVPGFAVDPSIRPEAIRLRSAG
                     GGRPQQARGPRRPGAAASANGWRPRPGRRPQGRPSGPASGQRGGSWVSLPGERTHR"
     gene            4011..4343
                     /locus_tag="VIN32_06935"
     CDS             4011..4343
                     /locus_tag="VIN32_06935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8922346.1"
                     /translation="MTAPCIHGFRTEECTSCRTCPHGLVTSRCGRCIRASSTPAGRKA
                     LLVARGEHPPEERAGFEIVYVPELSGWQVRSDDAPISDESYRSIFLARKAADDLAAAA
                     SAKESRAD"
     gene            complement(4396..5613)
                     /locus_tag="VIN32_06940"
     CDS             complement(4396..5613)
                     /locus_tag="VIN32_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HEY8922347.1"
                     /translation="MNLHSATVTRRRFLAALGGGVAAVVLAACGIERSAQPTPSASAP
                     PSATASPTDTPSPQPSPTPGPSLRDRIAQMLLVGFRGLTVEAAGEIAADIHDRNLGGI
                     VLFDTDQPTHSKVRNVESAAQLTALIDGLQALAPTPLLVAIDEEGGLVARLDQRHGFP
                     ATISAADLGARGDVTFTRQAGERIAETLASVGVNLNLAPVVDLNVNPTNPIIGALGRS
                     FSADPSVVTTQAEAFVEGHRSVGVRTTLKHFPGHGSSTSDSHLGVVDVTNTWSPIELD
                     PFRNLVHDRLADAVLTAHVFNSKLDPEHPATLSAPTITGILRQELGWDGVVISDDMQM
                     GAIRDAYGYADAVRLAILAGVDLLTIAQQQVYEPGIVASTIDLIEGFVNDGSLTEARI
                     DESYQRIQALKLR"
     gene            complement(5804..6760)
                     /locus_tag="VIN32_06945"
     CDS             complement(5804..6760)
                     /locus_tag="VIN32_06945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8922348.1"
                     /translation="MHRFSRPAALIAALSMLVTSAGLASGHPSSGPLSSGTEVLPIFR
                     GHVEHAKPNRPGRINNLSSRGGAIQATPKVYISWWGPEWGSGFSTGGHSSGQAQAYNT
                     DFFSSVGGSAWNNVVKQYCQNVPSGTQDCASVSGAVHITNPAGQFAGAWNDTTAVPSN
                     PTQSDIFAAAQRLRAHFTYSANATYMVYTPHLKSMSGFGTQWCAWHSSGSTASGQLAY
                     AYIPYMPDAGASCGANFVNTSSNTYGNGWFDGFSVVAGHEYSEAETDPFPSSGWVDRQ
                     GAENADKCAWSASSGNISLGSHQYAVQPTWSNATGGCAMSAR"
BASE COUNT          994 a         2302 c         2488 g         1143 t
ORIGIN      
        1 tcgcttaccg ctgcttcctt ccggacctga cggggttcac ggactctcgc tgcgtgggac
       61 ccgactctca acgtcggtcg atgttcgtag gggacgaagg cggatcggcc tcggccgggg
      121 attcagtccc gctgtagcgg attgcgggtg cagggcaccg ctagttcccc acctagcacg
      181 gccgccgcga tggtaggcgc gctcggcgag atcggtcaat cgagtcgtgg cggagagggg
      241 gggattcgaa cccccgacgg gttgccccga accgcatttc caatgcggcg ccataggcca
      301 ctaggcgacc tctccggggg cgtggcgcgt ggcggagagg gtgggattcg aacccacggt
      361 gctcatcgca caccgctttt cgagagcggc accttaaacc actcggacac ctctccggct
      421 ggggagtcta gcagcggagg gtcaggccgg agccggggcg ctggctgtca ggcggcgagc
      481 ctcgtcttcg cgcacgccgg agatgacgga gatctgatgc ggcatgccgg gggcgcgcgc
      541 cagctgcacg gcaccgccgg cggcgcctcg cttctcatcc ggaacggcat acaccaacgt
      601 gcggatctgg gcctcgagga gcgcgccgac gcacatcgag cagggctcgt gggtcgtgaa
      661 gacggtcagt tcggggaggc gcttggcgtc cagcttgcgc gccgccgcct gcagggtgag
      721 gagcacggcg tgagcggtcg ggtcgcgctg gaggaggatc cgacggtggt cgcgagcgat
      781 catggcgtcg tccaggaccg ccaccgcgcc gatcggcgtc tcgccctcct cggcgccgcg
      841 ccgagcctcg gcgatcgcct cggccatgta cagctcgtag ttcaccgttc cgagtctacc
      901 agcccctcca gggacctctg cgctcgctag catggaccgc ggccaccgac tggccgctct
      961 cctgggcagc acgaaagggg gcaagccatc caccaactgc cggcctgggc gcgccgcgat
     1021 tggattgccg tggcgatcgt caccgtcggt gctgccgtgg tccgcctggc aggcctgacg
     1081 acccccggcg gtctcgtctt cgacgagatc ttctatgcgc gcaatgcctg ccgcttcgtc
     1141 atcgacacgg cgcaatgcgg aatcgacgac ctcgtgagcg gcgcccaccc gccactcggc
     1201 aactggctga tcgggatcgg catcaggctc ttcggctacg acgagttcgg ctggcgcatc
     1261 gcgtcggcgg cggcgggcac gctgagcgtg gcgttgctct acgtgctcgt gcgcaggctg
     1321 ctggccgggc acgtgccggc cacggccgcg acgggcggcg ccacggcagc cgccgccctg
     1381 ctggccacgg acttcctgga cctcgtgcag tcgcgcgtcg cgatgctcga ctcgttcgtg
     1441 accatgctcg tcatcgccgt ggtcctgtgc gtggtcctgg acggcggtcg ccgccgtggg
     1501 ccgcgcggcg aggacctcgg cgcaccgcgc tggctgcgac tcctgtcgct gggccggccc
     1561 tggaggctgg cagccggcct cgcgctcggg gcggcggtgg ccgtgaagtg gtccggcggc
     1621 tacgtggcgt tcggcctcat cgcgctgctc gtggcctggg aagtcgcggc ctccgcgtcg
     1681 cggccgggcg acgatcagcg ccctggctgg cgggcagccg tcggtgacgc gttccggcgc
     1741 gaggccatcc cgtcgctggt cctgctgggc gttgtgccgg tcatcgtcta cctcgcctcg
     1801 tacaccgggc gtgtggacgg cgacctgctc gcgctcccct ggcgcgcggg atctgtctgg
     1861 cgcgggatcc tggagcacca gctggcgatg cttcgcttcc acctcggcct ggccggcgat
     1921 catccgtacg agtcggcgcc gtggtcgtgg atcctgctcg agcgcccggt cgcgtactcg
     1981 tttgcatcgt cttcgaacgc ctaccgcgag gtcctcgcca tcggcaaccc gctcacctgg
     2041 tggcccggag ctgtcgcgct cgtggcggtc ggggtgcgct gggttcgatc gggtgccagc
     2101 ctggctcgtc cagaggtcgt gctgctggtg gcggcgctct cgacgtacct gccctggctg
     2161 ctcctgtcag ggtcacgctc gcaggtcttc atctggtacc tgctcccaac gatcccattc
     2221 ctgtacgcgg cgctcggcct gtacacggcg ctggcgtgga ggtcggtgac ggggcggatc
     2281 gcgaccggcc tggcggcggt tgccatcgcc atctcattcg ccttcttcta cccgatcctg
     2341 acggccgctc cactgactcc agccgagtgg cgttcgcaca tctggttcgc cgattgccgc
     2401 cgtccaggcg cgccgaccct ggtgattccg gacgacgaga tcaaccgcgg gccacccccg
     2461 tcggggtggt gctggatcta gcggtgcgtc cgctcgccgg ggaggctgac ccacgaaccg
     2521 ccgcgctggc cggaagccgg cccgctcggt cggccctgtg gacgacggcc gggacgcggc
     2581 cgccagccgt tcgccgatgc ggcggcgcca ggacggcgcg gtccgcgcgc ctgctgcggt
     2641 cgtccaccgc cggcgctccg cagacggatc gcctcgggac ggatggacgg gtcgaccgcg
     2701 aagcccggga cgacctcgga tgggatgggc cggccgagca ggcgctcgat ctcgcgcagc
     2761 atcggcgcct catcgatgca gacgagcgag atggcgtcgc cctcgagccc cgcgcgtccg
     2821 gtgcgcccga tgcgatgcag gtagtcggag gggaccatcg gcagctcgta gttgacgacg
     2881 tgcggcagct gttcgatgtc cagcccgcgc gccgccactt cggtggcgat cagcagcgtc
     2941 acgcgtccgg ccttgaagtc gttgagggcg cgcactcgct gcggctgtga cttgttgccg
     3001 tggatcgccg cggtggcgat gccatcctcg agcagctgct tggcaagccg atcggcgccg
     3061 tgcttggtgc gggtgaagac gagtgcctgg tcgaccgcgt gcgtgcgaac caggtgcgag
     3121 aggagctcgc gcttgcgagc tcggtcgacg gggtgcacga cctggcgcac caggtcggtg
     3181 ggcgtgttgc gcggcgtgac ctggaccgat gccggacgat cgagcaggcc ttcggccagg
     3241 gctcggatct cgttcgagaa ggtggcgctg aagaggaggt tctgtcggcg tgggggcagc
     3301 agggcgatga tcgagcggat gtcgcggatg aagcccatgt cgagcatgcg atcggcctcg
     3361 tccagcacca ggatctcgac agcgccgaga tcgatcgtcc gctgccgtga gtggtcgagc
     3421 agccgaccgg gggtcgcgac gacgatctcc gggccgttgc gcagcgcgcg cagctgaccg
     3481 gggaagccga cgccgccgta gatggtggtc gagcgacggc tgttcgagcc gtagacgcgg
     3541 acgctctcct cgacctggat cgccagctcc cgggtgggga caagaatcag cgcacgaatg
     3601 cgcggccgcc cgccgctgcg cggtggcgtc gtggcgagca gctgcagcat cggcagcacg
     3661 aaggccgccg tcttgccggt gccggtctgg gccccggcca gcaggtcgcg cccggcaagg
     3721 acgaatggga tcgcctcgcg ctggacggga gtaggttcgg tatagccctg cgccgacacg
     3781 gcgcggagca gtccgggcgc aagcccgagc gttgagaagg acatgagaac tcctgacgag
     3841 gccctcccgc tgcaatgcgg ggcccggtca gggcgaagga ttggatcggg atcgggacgg
     3901 ggttgaagcc gcggcgcgga ccggagcgcc tatcgaacac gttttcgaaa tgatacacga
     3961 tcggcgtcga agggcggaag tggtaccctt ggggccccac gaaggacgcc atgacggccc
     4021 cctgcatcca cggattccgc actgaagagt gcacctcctg ccgaacttgc ccccacgggc
     4081 ttgtcacctc ccgctgcggc cgatgcatcc gagcgtccag cacgccggcc ggccgcaagg
     4141 cgctcctcgt cgcacggggc gagcatcccc ccgaggagcg cgccggcttc gagatcgtgt
     4201 acgtgcccga gctcagcgga tggcaggtgc gctccgacga cgctcccatc tcggacgagt
     4261 cctatcggtc catcttcctc gcccgcaagg ccgccgacga cctggctgcg gctgcatccg
     4321 cgaaggaatc tcgcgccgac tgaacctccg ctgcgcggac aagtacctct acttcctcga
     4381 ggcacacctg caggactagc gcagcttcag cgcctgaatc ctctggtacg actcgtctat
     4441 gcgcgcctcg gtcaggctgc cgtcattgac gaagccctcg atcaggtcga tcgtcgatgc
     4501 cacgatgccc ggctcataga cctgctgctg ggcgatggtc agcagatcga ctcccgccag
     4561 gatcgccagg cgcaccgcgt ccgcataccc atacgcgtcg cgtatggcgc ccatctgcat
     4621 gtcgtctgag atcaccaccc cgtcccagcc gagctcctgt ctcaggatcc ccgtgatcgt
     4681 gggtgcggaa agggtggcgg gatgctcggg atcaagcttg gagttgaaga cgtgcgcggt
     4741 cagcacggca tcggccagcc ggtcgtggac caggttgcgg aacgggtcga gctcgatcgg
     4801 cgaccaggtg ttggtgacat cgacaacccc gaggtgagag tcagaggtgg agctgccgtg
     4861 accggggaag tgcttgagcg tcgtccgcac ccccaccgag cgatgtccct ccacgaacgc
     4921 ctccgcctgc gtcgtgacga ctgacgggtc cgccgagaag gagcgaccca gcgcaccgat
     4981 gatcgggttg gtcgggttca cgttgaggtc gaccaccggg gcgaggttga ggttgacgcc
     5041 gaccgatgcg agcgtctcgg cgatccgctc ccccgcctgc cgtgtgaagg tcacgtctcc
     5101 ccgtgcgccg aggtctgcgg cggagatggt cgcggggaag ccgtggcgct ggtcgaggcg
     5161 cgccaccagg ccgccctcct cgtcgatcgc aaccaggagc ggcgtggggg cgagggcttg
     5221 gaggccgtca atgagcgccg tcagctgggc cgccgactcg acgttgcgga ccttgctgtg
     5281 ggtcggctga tcggtgtcga agaggacgat cccaccgaga ttccgatcgt ggatgtccgc
     5341 cgcgatctcg cctgccgctt cgacggtcag cccgcggaag ccgacgagca gcatctgcgc
     5401 aatccggtcg cgcaggctcg gacccggcgt gggcgatggc tgcggcgacg gggtgtcggt
     5461 ggggctcgcc gtcgcggagg gaggggcgga ggcggatggg gtcggctggg ccgatcgctc
     5521 gatcccgcag gcggccagga cgaccgcggc cactcccccg ccgagcgcgg caaggaaacg
     5581 gcgccgggtg accgtggccg aatggagatt cacgctatca gcctagccgc gcgtcggtga
     5641 atatcaggcg aacgtagaac gaagagatgg agacgcacga cggagctggg caccgcgcgg
     5701 gacgcccggc tcccgtcgat ggtctgggcc aatccccatc tcgagatcgc tgtgaaagcg
     5761 aaagaggccc cggagggcct ctcttcgcgg gggtgggttg cgactacctt gctgacatcg
     5821 cgcagccgcc ggtggcattg ctccacgtcg gctgaacggc gtattggtgg gatccgagac
     5881 tgatgttccc cgacgaggcg ctccacgcgc acttgtccgc gttctcggct ccctgccggt
     5941 cgacccaccc gctgctcggg aagggatcgg tctcggcctc cgagtattcg tggccggcga
     6001 cgacgctgaa cccgtcgaac cagccgttgc cgtacgtgtt gctgctcgtg ttgacgaagt
     6061 tcgcgccgca cgacgcaccg gcatcgggca tataggggat gtacgcgtac gcgagctggc
     6121 cgctggccgt gctgccggat gagtgccagg cgcaccactg cgtcccgaag ccgctcatcg
     6181 acttgaggtg cggtgtgtag accatgtagg tggcgttggc gctgtaggtg aagtgcgccc
     6241 tcaacctttg cgcggccgcg aagatgtcgg actgggtcgg gttcgacggc accgcagtgg
     6301 tgtcattcca cgctccagca aactggccgg cggggttggt gatatgcacc gcgccactca
     6361 cggacgcgca gtcctgcgtt ccggatggaa cgttctggca gtactgcttc accacgttgt
     6421 tccaggcgct gccgccgacg ctgctgaaga agtcggtgtt gtaggcctgg gcctgaccgc
     6481 tggagtggcc gccggtgctg aagccgctgc cccattccgg gccccaccag ctgatgtaga
     6541 ccttgggcgt cgcctggatc gcgccgccgc ggctggagag gttgttgatg cgtcccggcc
     6601 tgttcggctt cgcgtgctcc acgtggccgc ggaagatcgg cagcacctcg gtacccgaac
     6661 tgagcggccc agacgacgga tgtccacttg ccaggccggc gctggtgacc agcatcgata
     6721 gggcggcgat cagtgcggca ggacgcgaga agcgatgcat cgacgactct ctctcttctt
     6781 acgggaggtg cgcgatcggg ggcggggcgc gaacggaaga gtacagcccg aaggtgccga
     6841 tgcgccagat ctccagacac cactcgaccg caccatggtg cagctggtcg ccgcggagtc
     6901 gtgcgcgatt gcctcgattc acgccgc
//
LOCUS       DATCQR010000209         2282 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_89654, whole genome shotgun sequence.
ACCESSION   DATCQR010000209 DATCQR010000000
VERSION     DATCQR010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89654"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..152)
                     /locus_tag="VIN32_06950"
     CDS             complement(<1..152)
                     /locus_tag="VIN32_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011647250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HEY8922349.1"
                     /translation="MGQAFRSHTCGELRASHVGERVKLSGWVHRRRDHGHLAFFDLRD
                     RYGITQV"
     gene            complement(181..1449)
                     /gene="hisS"
                     /locus_tag="VIN32_06955"
     CDS             complement(181..1449)
                     /gene="hisS"
                     /locus_tag="VIN32_06955"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEY8922350.1"
                     /translation="MSRPVSAPRGTRDLLPEDGPAWSHVEGIARDLSSRYAFDRIETP
                     LFERIELFSRGLGESSDAVEKEMFLVGGAAGSEEERAEWALRPEPTAGIVRAYLEHGM
                     HVRPGPQRLWMIGPMFRYDRPQAGRFRQFVQWDVEVIGDPGPMVDAEIIELGHRFFAE
                     AGVSDVVAYVNSIGDATCRPGYRAALVEYFGAHEDRLTDDSRRRLRDNPLRILDGKDL
                     DPELAAAAPRSVDHLCDPCRAHFAAVQALLDGLGVRYEVDNRIVRGLDYYTRTTFEFY
                     VAGRRGQQQALGGGGRYDGLVELLGGPLTPGIGFGIGIDRTVMAVAEQGAVPPAEDPL
                     VAVVGADPDALRQRLAVAGTLRAAGLRVRADGSSRKLGKQLESAAKVGAHYAVLVDPA
                     LPTGRVILRDLEASEQREILLDEVPAAIAT"
     gene            1488..>2282
                     /locus_tag="VIN32_06960"
     CDS             1488..>2282
                     /locus_tag="VIN32_06960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8922351.1"
                     /translation="MRTDRARRLLPILVLIVLLAAGLWIRLAVLDAKGHYGDALVNGR
                     WADHMARYGPWDFYRHDGAIYPALLYAFWPIGVLLDGAAQARAIKGLSIPFDLAIGVV
                     AYLAARRMVGPARALVAPAIYLFNPAVLLAGPVWGQVDAAGALAYLLALLALAGRRFA
                     IAGASAVVALLIKPQFGLVLLPVALMAILTWRATRSRAPVVRALLGGAAAYLVVALPL
                     RMDPISYVGRIIGAGSFKEMSSANAANIWGLIAGYKHPDGGLVYVGG"
BASE COUNT          327 a          801 c          786 g          368 t
ORIGIN      
        1 acctgggtga tcccgtaccg gtcgcgcaag tcgaagaagg cgagatggcc gtgatcgcgg
       61 cgccgatgca cccagccgga gagcttgacc cgctccccca catgggaggc gcgcagctcg
      121 ccgcaggtat ggctccgaaa ggcttggccc atggtcggca gatggtagcc gaccctccga
      181 tcacgtcgcg atggccgcgg gcacctcgtc gaggaggatc tcgcgctgct cgctggcttc
      241 gaggtcccgc aggatcacgc gcccggtcgg cagggcggga tcgacgagca cggcatagtg
      301 agcgccgacc ttggcggcgg actcgagctg cttgccgagc ttgcgggagc tgccatcggc
      361 gcgaacgcgg aggccggctg cgcgcaacgt gcccgcgacc gccaggcgct gtcggagcgc
      421 atctggatcg gcccccacga cagcaaccag cggatcttca gcgggtggca cggcgccctg
      481 ctcggccact gccatgacgg tccggtcgat gccgatgccg aagccgatcc ctggcgtcaa
      541 cgggccgccc aggagctcga ccagcccgtc gtagcggcca ccgccaccca gcgcctgctg
      601 ctggccacgc cgcccggcta cgtagaactc gaaggtggtg cgcgtgtagt agtcgaggcc
      661 acgcacgatg cggttgtcga cctcgtagcg cactcccagc ccgtcgagca gtgcctggac
      721 cgccgcaaag tgcgctcggc atgggtcgca caggtggtcc acggagcggg gggctgcggc
      781 ggcgagctcc ggatcgaggt ccttgccgtc gagaatgcgc agcgggttgt cgcgcagcct
      841 ccgtcgcgag tcatcggtca ggcggtcttc gtgcgctcca aagtattcga ccagcgcggc
      901 tcggtagccg ggacggcacg tcgcgtcgcc gatcgaattg acgtaggcga ccacgtcgga
      961 cacccctgcc tcggcgaaga agcggtggcc gagctcgatg atctcagcgt cgaccatcgg
     1021 gcccggatcg ccgatcacct cgacatccca ctgcacgaac tgcctgaagc ggccggcctg
     1081 ggggcggtca tagcggaaca tggggccgat catccacagg cgctgcgggc cgggccgcac
     1141 gtgcatgccg tgctcgaggt aggcccgtac gatcccggcg gtcggctccg gtcggagcgc
     1201 ccactcggcc cgctcctcct cgctgccggc tgccccgccg acgaggaaca tctctttctc
     1261 gaccgcatcc gacgactcgc ccagtccccg gctaaagagc tcgatccgct cgaagagcgg
     1321 cgtctcgatg cgatcgaatg catatcgact gctcaggtca cgcgcgatcc cctcgacatg
     1381 gctccaggcg gggccgtcct cgggcagcag gtcgcgcgtg ccgcgcggcg cggagacggg
     1441 gcgggacatg gcgcgagtct acgggcctgc gctagcctgc gccggtgatg aggacagatc
     1501 gcgctcgaag gctgctcccg atcctcgtcc tgatcgtgct tctggcagcc ggcctttgga
     1561 tccgcctggc cgtcctcgac gcgaagggcc actacggcga cgcgctcgtc aacggtcgct
     1621 gggcagacca catggcccga tacggcccgt gggacttcta ccgacacgac ggggccatct
     1681 atccggccct cctctatgcg ttctggccca tcggcgtgct gctggatgga gccgcgcagg
     1741 ctcgcgccat caagggcctc tcgatcccct tcgacctggc cattggcgtg gtcgcctacc
     1801 tggcggcgcg tcgcatggtc ggaccggctc gtgccctcgt cgctccggcg atctacctct
     1861 tcaatcctgc cgtgctcttg gcgggaccgg tatggggcca ggtcgacgcc gccggagcgc
     1921 tggcctatct cctcgccctg ctggcgctgg cgggtcgtcg cttcgccatc gcgggcgcga
     1981 gcgcggtcgt ggcgctgctg atcaagccgc agttcgggct cgtgctgctg cccgtcgccc
     2041 tgatggcgat cctgacctgg cgggcaaccc gcagtcgcgc ccctgtagtg cgcgccctcc
     2101 tgggaggagc cgccgcctac ctggtcgtgg cgctgccact gcgcatggac ccgatctcgt
     2161 acgttggccg gatcatcggt gcggggagct tcaaggagat gtcgtcggcc aacgctgcga
     2221 acatctgggg cctgatcgct ggttacaagc accccgatgg cggactcgta tacgtgggcg
     2281 gc
//
LOCUS       DATCQR010000210         6070 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_89657, whole genome shotgun sequence.
ACCESSION   DATCQR010000210 DATCQR010000000
VERSION     DATCQR010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6070
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89657"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..357)
                     /locus_tag="VIN32_06965"
     CDS             complement(<1..357)
                     /locus_tag="VIN32_06965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8922352.1"
                     /translation="MAWLTDTDRRLMAVTWAVKLGVLLVGAITYVSFRNGVIDSPGDI
                     LHMWSHWDAPHYLDLVVFGYRAGDSSALVGPHGYRSIYPDDLALYIVFYPLFPWLASI
                     VNAIVGDPLVSVFVVTT"
     gene            431..859
                     /locus_tag="VIN32_06970"
     CDS             431..859
                     /locus_tag="VIN32_06970"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY8922353.1"
                     /translation="MPSAKHSVTVRRPVAEVFAFVADGESAMRWRPAVLDVSHQSGEG
                     LGAIYLQGVKGSGGRRIAADYEITAVELGRRIAFRAIAGLVRPTGEYQFAADGEGTTV
                     SLALDATLTGWKRLLMGRAVQGTMESEVHNLEALKTILEA"
     gene            863..1192
                     /locus_tag="VIN32_06975"
     CDS             863..1192
                     /locus_tag="VIN32_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018657483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8922354.1"
                     /translation="MDRRPVSSATQRRYDEIVAGLEDAGVKASSMFGMPTLKRNGKAI
                     GGLWGDAMTFKLAPEALAEALRIEGAQQFDPSGMGRPMKAWVVVPVAQSGEWKKLAQL
                     ALAASDT"
     gene            1240..1316
                     /locus_tag="VIN32_06980"
     tRNA            1240..1316
                     /locus_tag="VIN32_06980"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1274..1276,aa:Pro,seq:cgg)
     gene            complement(1340..1891)
                     /locus_tag="VIN32_06985"
     CDS             complement(1340..1891)
                     /locus_tag="VIN32_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013568732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEY8922355.1"
                     /translation="METGFIAETTAVLRRTPDVLRSLLVGLPEAWTDTPDAADGWRPR
                     DVVGHLITGEETDWITRTRRILEQGTSVPFDRFDRVAMLERDAGVPLDDLVERFASLR
                     AENLAALDDLVTDADLDRRGLHPSLGEVTLRELLATWAVHDLDHVGQIHAGLAGSHDA
                     EVGPWKAYLGILLRREDPAAVPG"
     gene            1959..2498
                     /locus_tag="VIN32_06990"
     CDS             1959..2498
                     /locus_tag="VIN32_06990"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8922356.1"
                     /translation="MQADAAIRSERLELPLLSLDQLDRLGAGDGASVGADLHAILPIP
                     WLDEVRWLAGMRAQQLRLQPDDAPWLLRPILLRTGDGGLQAIGYLNFHAGPDERGMVE
                     VGYTLLPEARGHGYAIEAVRAAFDWATSVHGVHLFRASVAPDNERSLNLIIKLGFRQT
                     GEQWDEVDGLELVFELERP"
     gene            complement(2637..3527)
                     /gene="panB"
                     /locus_tag="VIN32_06995"
     CDS             complement(2637..3527)
                     /gene="panB"
                     /locus_tag="VIN32_06995"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007575303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEY8922357.1"
                     /translation="MTTSHTNVGESQVPAKLAITELAEMKRQGAKIAMVTAYDAAGAR
                     LAEAAGIDVVLVGDSAAMVVLGHDSTVPVTMDEMLFMTRAVASAVQRPLVVGDMPFGS
                     YQVSDREAVRNAIRFVKEGRADTVKLEGAGRMLPRVRAIVESGIPVMGHLGLTPQSAT
                     ALGGYKAQGRTAARARRMLADALALQEAGCYSLVLEAVPTPVAARITEALEIPTIGIG
                     AGPQVDGQVLVYHDLLGLTEGHTARFVKRYADLATVIREALASFAADVRSGAYPEERH
                     TYGMPADEQAAFEAEISQEQ"
     gene            complement(3531..4340)
                     /gene="panC"
                     /locus_tag="VIN32_07000"
     CDS             complement(3531..4340)
                     /gene="panC"
                     /locus_tag="VIN32_07000"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018171287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEY8922358.1"
                     /translation="MKVVRGVADLRGALDPGPGRAPSIGLVPTMGALHEGHASLIAAA
                     REGSELVVVSLFVNPAQFGRGEDLEQYPRDETRDIATAEAAGVDILFAPDAEQMYPDG
                     FDTWIDPGKVGSYLEGSSRPGHFRGVATICTKLFLLVKPKRAYFGRKDAQQVAVVKQL
                     VRDLELPVEIVACPTVRDADGLALSSRNVFLSPEERQVALALPRALQAGLGAHRSGTD
                     AVRAAREVLDAEPELRVDYVAEADLDGPTLAAAVVVGSTRLIDNVLLEGEA"
     gene            complement(4337..5011)
                     /locus_tag="VIN32_07005"
     CDS             complement(4337..5011)
                     /locus_tag="VIN32_07005"
                     /inference="COORDINATES: protein motif:HMM:NF022188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HEY8922359.1"
                     /translation="MISTVRVVGTGRAGSAIASRLAERGLIVRAGREHLADADLVLLA
                     VPDEAIAEVARQVPIGPWLAHVSGATSLQALEPHTLRFSVHPLQTLSRERGPEQLDGA
                     FAAVTGETEDAREVARWLAETLGLLPFDLADADKPLYHAGAAMASNFLVTLHRAAARL
                     MDESHAPPEALLPLMTRTIENGFVLTGPIARGDWSTVEAHLHALEERAPDLVPLYRAL
                     AEATRP"
     gene            complement(5043..5312)
                     /locus_tag="VIN32_07010"
     CDS             complement(5043..5312)
                     /locus_tag="VIN32_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018335385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UBP-type zinc finger domain-containing protein"
                     /protein_id="HEY8922360.1"
                     /translation="MSPEPICRHLHGVYPVAARGQGCEECLATGGRWLHLRRCLHCGH
                     VGCCDQSPGRHASGHAREAGHPIVESMAPNEDWRWCFVDEVYLEG"
     gene            complement(5309..>6070)
                     /locus_tag="VIN32_07015"
     CDS             complement(5309..>6070)
                     /locus_tag="VIN32_07015"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEY8922361.1"
                     /translation="VLTNAHNLHGDEVHVFFADGRDAEGKVLGADGDGDLAVIGVDTG
                     DAPAIAWGDGSALSIGSPLVALGNPAGNGLRVTVGFVSGIGREFRGPRGRRVAGAVEH
                     TAPLLPGSSGGPIVDGDGKLLGINTNRLGSGFYQAISADAALRDRVAALGRGEEPKRR
                     RLGLGLAPAHVARQLRRAVGLDERDGLLVREVEDGSPAAKAGIAEGDLIVAAAGKPVA
                     SADDLFEAMGSVAADAALPLTVLRGSEERTVTIAA"
BASE COUNT          951 a         2123 c         2105 g          891 t
ORIGIN      
        1 ggtcgtcacc acgaacacgc tgaccagcgg atcgccgacg attgcgttca cgatgctcgc
       61 cagccacggg aagagcgggt agaagacgat gtacagcgcc aggtcgtcgg ggtagatgga
      121 acggtaacca tgcggcccga cgagggcgct ggaatccccg gcccgatatc cgaagacgac
      181 gaggtccagg taatgcggag catcccagtg gctccacatg tgcaggatgt cccccggcga
      241 gtcgatcacc ccgttgcgga acgacacgta ggtgatcgcc cccaccagca gcacgcccag
      301 cttgaccgcc caggtgacgg ccatcagccg gcgatcggtg tcggtgagcc acgccatcgc
      361 ggcagtgtag gggcgagcca tcggcggccg cgacgcgcta ccctcaacgc acacgaggac
      421 tggagcgacg atgccatcgg cgaagcattc ggtgacggtc aggcggccgg ttgccgaggt
      481 attcgcgttc gttgccgacg gagagagcgc catgcgctgg cgtcccgccg tcctcgatgt
      541 ctcacatcag tccggtgagg gactcggcgc catctacctc cagggagtca agggatcggg
      601 cggacgacgc atagccgccg actacgagat caccgccgtt gagcttggtc ggcggatcgc
      661 gttccgggcg atcgccggtc tagtcagacc gaccggggag tatcagttcg cagcggacgg
      721 ggaaggcacc accgtgagcc tcgccctcga cgcaacgctg accgggtgga agcgactcct
      781 gatgggtcgc gcggtgcagg gcaccatgga aagcgaggtc cacaacctcg aggcgctcaa
      841 gacgatcctt gaggcctgag ccatggatcg acggcccgtc tcgtccgcga cgcagcggcg
      901 ctacgacgag atcgtggctg ggctcgagga cgccggggtc aaggcaagca gcatgttcgg
      961 gatgccaacg ctcaagcgca acggcaaggc gatcggcggc ttgtggggcg atgcgatgac
     1021 cttcaagctc gcccccgagg cgctcgccga ggcgctccgc atcgagggcg cgcagcagtt
     1081 cgatccttcg ggcatgggcc ggccgatgaa ggcctgggtg gtcgtccccg tcgcacagtc
     1141 gggggagtgg aagaagctcg cccagctcgc cctggccgcc agcgacacct agcaaggcgg
     1201 ctggtagact gcgcctccca tcggaacccg gtggacggtc ggggcgtggc gcagttaggt
     1261 agcgcgcatc gttcgggacg atgaggtcgg aggttcaaat cctctcgccc cgaccagctc
     1321 cagcccgcag cagagcccgc tagccgggga ccgccgccgg atcctctcgt cgcaacagga
     1381 tccccaggta cgccttccag ggccccacct cggcgtcgtg cgagccggcg aggccggcat
     1441 ggatctggcc gacgtggtcg aggtcgtgga cggcccatgt cgcgagcagc tctcgcagcg
     1501 tcacctcgcc gagggacgga tgcaggccgc gccgatcgag atcggcgtcg gtgaccaggt
     1561 cgtcgagtgc ggcaaggttc tccgcgcgaa gcgacgcgaa tcgctccacc aggtcgtcaa
     1621 gtgggactcc tgcgtcgcgc tcgagcatcg cgacgcgatc gaagcggtcg aacgggaccg
     1681 atgtcccctg ctccaggatc cgccgcgtgc gggtgatcca gtcggtctct tcgccggtga
     1741 tcaggtgccc gacgacatcg cgcgggcgcc agccatctgc ggcatcgggc gtatcggtcc
     1801 aggcctccgg caggccgacc agcaggctgc gcaggacatc gggggttctc ctcagcaccg
     1861 cggtcgtttc ggcgatgaac ccggtctcca ccggccgagt tagccaccac cggcgggtcg
     1921 cgtcaagcgg cattgtgccc tcctgtaggc tggccgccat gcaggcggac gcggcgatcc
     1981 gatcagagcg cctggagctg ccgctcctct cgctggatca gctggatcgc ctgggcgccg
     2041 gagacggggc gagtgtcgga gccgacctgc acgcgatcct gccgattccg tggctcgacg
     2101 aggttcgctg gctggcgggc atgcgcgccc agcagctcag gctgcagcca gacgacgcac
     2161 cgtggctgct gcgaccgatc ctgctgcgca ctggggatgg tggtcttcag gcgatcggct
     2221 atctgaactt ccatgccggc ccggacgagc gcggcatggt ggaggtcggc tacaccctcc
     2281 ttcccgaggc gcgcgggcat ggatacgcca tcgaggcggt gcgagctgcg tttgactggg
     2341 cgaccagcgt ccacggcgtc cacctgttcc gcgccagcgt ggcgcctgac aacgagcgat
     2401 cgctcaacct gatcatcaag ctcggcttcc gccagaccgg cgagcagtgg gacgaggtgg
     2461 acgggcttga gctcgtcttc gagctggagc gtccgtgatg ctgagcattc gcgacgtccc
     2521 cggcatctcg gtgggacatg cgaccgatgc cctcggcctc accggatgca ccgtcgtcct
     2581 ggccatatgc tcggatgcgg agcagcccgc gccggactcg cggaaggcag cgggggctat
     2641 tgctcttggg agatttccgc ctcgaaggcg gcctgctcat cggccggcat gccgtacgtg
     2701 tggcgctcct ctgggtaggc gccagatcgg acatcggcgg cgaaggaggc cagcgcctcg
     2761 cgaatgaccg tcgccaagtc cgcgtagcgc ttcacgaacc gcgcggtgtg cccctcggtc
     2821 aggcccagca ggtcgtgata gacgaggacc tgcccatcga cctgcggccc cgcgccgatc
     2881 ccgatggtcg ggatctcgag tgcctccgtg atccgcgcgg cgacgggcgt cgggaccgcc
     2941 tcgagcacga gcgaatagca gccggcctcc tggagagcca gcgcatcggc cagcatcctg
     3001 cgtgcccgcg cggccgtgcg tccctgcgcc ttgtagccgc ccagcgccgt ggccgactgt
     3061 ggcgtcaggc ccaggtgacc catcaccggg atcccggact cgacgatggc ccggacgcga
     3121 ggcagcattc gccccgcccc ttcgagcttg acggtgtctg cgcgtccctc cttgacgaag
     3181 cgaatggcat tccgcaccgc ctcacggtcg gacacctggt aggagccgaa gggcatgtcg
     3241 ccgacaacca gcggccgctg cactgcggac gcgacggcgc gggtcatgaa cagcatctcg
     3301 tccatggtca ccgggaccgt cgagtcgtgg cccaggacca ccatcgcggc actgtcgccg
     3361 accagcacga cgtcgatgcc ggccgcctcg gccaaccgcg ctcccgcagc gtcgtaggcg
     3421 gtcaccatcg cgatcttggc gccctggcgc ttcatctccg ccagttcggt gatggcgagc
     3481 ttggctggga cctgggattc gccgacgttg gtgtggctgg tggtcatgcg ctatgcctct
     3541 ccctcaagaa ggacattgtc tatcagacgc gtcgatccga ctacgaccgc ggcggccagg
     3601 gttgggccgt ccaggtccgc ctcggccacg tagtcgacgc gcagctccgg ctcggcgtca
     3661 agcacctcac gggcggcgcg caccgcatcc gtcccggacc gatgcgctcc gagccccgcc
     3721 tgcagcgccc gtggcagtgc aagcgcaacc tgccgctcct ccggtgagag gaagacgttg
     3781 cggctcgaca gagccaggcc gtccgcatcg cgcacggtcg gacaggcaac gatctcgacc
     3841 ggcagctcga ggtcccggac gagctgcttg acgactgcca cctgctgcgc gtccttgcgg
     3901 ccgaagtagg cacgcttggg cttcaccagc aggaagagct tggtgcagat ggtcgccacg
     3961 ccgcggaaat gtcccggccg cgaggagcct tccaggtagc tgccgacctt gccggggtcg
     4021 atccaggtat cgaagccgtc cggatacatc tgctccgcgt ccggcgcaaa caggatgtcg
     4081 accccggccg cctcggcggt ggcgatgtcg cgcgtctcgt cccgcgggta ttgctcgagg
     4141 tcctcgcctc gaccgaactg ggccgggttc acgaacaggc tgaccaccac cagctcggat
     4201 ccttcgcgcg cggccgcaat gagcgaagcg tgaccctcat gcagggcacc catcgtgggc
     4261 acgaggccga tgctgggcgc gcgcccgggt cccggatcca acgccccgcg caggtccgcc
     4321 accccgcgga cgaccttcac gggcgggtcg cctcggcgag ggcccgatat agcggcacga
     4381 ggtccggtgc gcgctcctcg agggcatgca ggtgcgcctc aaccgtcgac caatcgccgc
     4441 gggcgatcgg cccggtcagg acgaagccat tctcgatggt gcgggtcatg agcgggagca
     4501 gcgcctcggg cggcgcgtgg ctctcgtcca tcagccgcgc ggcagcgcga tgcagggtca
     4561 ccaggaagtt gctcgccatg gcggcgccgg cgtgatacag cggcttgtcg gcatcggcca
     4621 ggtcgaaggg gaggaggcca agggtctcag ccagccagcg agccacctcg cgagcatcct
     4681 cggtctcgcc tgtgacggcg gcgaaggctc cgtcgagctg ctcgggcccg cgctcgcggg
     4741 agagcgtctg cagcggatgg acgctgaagc ggagcgtgtg cggctccagc gcctggaggg
     4801 aggtggcgcc cgagacatgc gccagccatg ggccgatcgg cacctgccgc gcgacctcgg
     4861 cgatggcctc gtcgggcacc gccagcaaca cgaggtccgc atcggcgaga tgctcgcgac
     4921 cggcgcggac gatcaggccg cgctcagcca ggcgtgatgc gatggcgctg ccggcgcgcc
     4981 ccgtgccgac cacccggacc gtgctgatca tgcgggggag tctgccacac tgcccggctc
     5041 agctagccct cgaggtagac ctcgtcgacg aagcaccagc gccagtcctc gttcggcgcc
     5101 atcgactcga cgatcgggtg gcccgcttca cgggcgtgcc ccgacgcgtg tcggccgggc
     5161 gactgatcgc agcagcccac gtgcccgcag tgcaggcatc ggcgcagatg cagccatctg
     5221 ccgccggtcg ccaggcattc ctcgcagcct tggccgcggg ccgcaaccgg ataaacgcca
     5281 tggaggtggc ggcagatcgg ttcggggctc acgcggcgat ggtaacggtc cgctcctccg
     5341 agccccgcag gacggtgagt gggagggcgg catcggcggc caccgagccc atcgcctcga
     5401 acaggtcgtc ggcgctcgcc acgggcttcc cggcggcggc gacgatcagg tcgccctcgg
     5461 cgatcccggc cttcgcggcc ggcgagccat cctcgacctc acgcaccaac agcccatcgc
     5521 gctcgtcgag tcccaccgct cgccgcagct gccgcgccac gtgcgccggg gcaagcccca
     5581 gcccgagccg acgccgcttc ggctcctcgc cgcgccctag ggccgcgacc cggtcgcgca
     5641 gcgccgcatc cgcagagatc gcctggtaga agccgctgcc cagtcggttg gtgttgatcc
     5701 cgagcagctt cccgtcgcca tcgacgatgg gaccgccgga tgagccgggc agcaggggag
     5761 cggtgtgctc caccgcgcct gccacgcgac gccctcgcgg gccgcggaac tcccggccga
     5821 tgccggagac gaagccaacc gtgacccgca ggccgtttcc cgcagggttg ccgagcgcga
     5881 ccagcggcga gccaatgctc agcgcagacc cgtctcccca cgcgatggcc ggcgcatcgc
     5941 cggtatcgac gccgatcacc gccaggtcgc cgtcgccatc ggctccgagg acctttccct
     6001 ctgcgtcgcg tccatcggcg aagaagacat gcacctcgtc gccgtgcagg ttgtgcgcgt
     6061 tggtgagcac
//
LOCUS       DATCQR010000211         4223 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_166527, whole genome shotgun sequence.
ACCESSION   DATCQR010000211 DATCQR010000000
VERSION     DATCQR010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4223
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166527"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..890)
                     /locus_tag="VIN32_07020"
     CDS             complement(<1..890)
                     /locus_tag="VIN32_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY8922362.1"
                     /translation="MDLVVRNARVHPTGGKHPEPVDIGIEDGRIGAIRERGELASAAT
                     ELDAEGSLLSPPLVDPHVHMDAVLTVGEPRYNESGTLIEGILTWAERKPSLTHEDVKR
                     RALEAIRWEVAQGTGLIRSHVDVCDPNLVALKALLELRDEVRDIVDLKLIAFPQDGIL
                     SFPDGKELMREAMRLGCDVIGGIPHYEWTRDDGVEEVHFIFDLAKETGKPIDLHCDET
                     DDEQSRFLEVVAARAIRDGMQGRVVAGHTTAMASYNDNYAFKLIQILKRAGVTIVANP
                     LDNIVLQGRFDTYPKRRGMTR"
     gene            1240..1316
                     /locus_tag="VIN32_07025"
     tRNA            1240..1316
                     /locus_tag="VIN32_07025"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1274..1276,aa:Pro,seq:ggg)
     gene            1622..2266
                     /locus_tag="VIN32_07030"
     CDS             1622..2266
                     /locus_tag="VIN32_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8922363.1"
                     /translation="MNPFNRSALTILSLGLLVLSPSLASAATDRVTVENMRGPLVNAW
                     FSSTDQSGCIETDTFVTAQRTTDQLLPGRGTTAGIAAVSISQYDACTDTSLLQAVGQT
                     DSLGAAELQFSNQVDWASLHTTITVTNIETGDAFDVSVNVAWAGTSDITRDHSNTNDL
                     YGGGCHVLNRWKGSGRTADASGLVSDGATNFTPTTSQYAEIGVVIDGFEVIDCA"
     gene            complement(2357..2560)
                     /locus_tag="VIN32_07035"
     CDS             complement(2357..2560)
                     /locus_tag="VIN32_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013629052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HEY8922364.1"
                     /translation="MTTGTIKKVVSDRGFGFIAAEDGKEYFFHRDGLDRSLEFDRLVG
                     GEKVNFEVESSPRGPRAGKVSAA"
     gene            2764..4092
                     /locus_tag="VIN32_07040"
     CDS             2764..4092
                     /locus_tag="VIN32_07040"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY8922365.1"
                     /translation="MDPAPALPAGPPFTIRARLLTTLGSGGLRYEPDARVEVDGAGRL
                     ARVERWSAEADPGPSIDLRPWVMMPGLIDLHAHLPQLPNAGVGAGLDLLTWLERYIFP
                     LERNFDVPTAELIAPAAFRAFARGGTTTVVAYGALWADSLDACFRAAEAHGIRAVIGK
                     VMMDRLSYDTDHDPSEMLELSLRQSTDLCATWNGRDDGRLMYAFTPRFAVSCSAEMLR
                     ASALAANEAGAYWQTHLSEDREEISEVRGLFPDAIDYTDVYDRAGGLTPRAILAHAIH
                     LSDREVARLAESRAVVAHCPSSNLFLTSGAMPLTRYLAAGIRIGLGSDVAAGPDVQLF
                     SVMRAGAYTQNALRAAGLSDAPQLSPADWLRLGTLGGAEALGLQDRIGSIEVGKEADM
                     IVVDPSVTLPLEGVGAHDDGGPDEMLSRLVFRAHPSMVRGAWVRGRLLPA"
BASE COUNT          690 a         1414 c         1379 g          740 t
ORIGIN      
        1 cgggtcatcc cacgccgctt ggggtaggtg tcgaatcggc cctgcagcac gatgttgtcg
       61 agcgggttgg ccacgatggt gactccggcg cgcttgagaa tctggatcag cttgaaggca
      121 tagttgtcgt tgtaggacgc catcgcggtg gtatggcccg ccaccacccg accctgcatg
      181 ccatcgcgga tggccctggc cgccacgacc tccaggaagc ggctctgctc gtcatcggtc
      241 tcgtcacaat gcaggtcgat cggcttcccc gtttccttcg ccaggtcgaa gatgaaatgc
      301 acctcctcga cgccgtcgtc gcgggtccac tcgtagtggg ggatgccacc gatcacgtcg
      361 cagccgagcc gcatcgcctc ccgcatcagc tccttgccgt cgggaaagga gaggatcccg
      421 tcctgcggaa aggcgatgag cttcagatcg acgatatcgc ggacctcgtc gcgcagctcg
      481 agcagggcct tgagcgccac caggttcggg tcacagacgt cgacgtgcga tcgaatcagg
      541 ccggtgccct gagccacttc ccatcgaatc gcctcgagcg cgcgccgctt gacatcctcg
      601 tgggtcagcg acggcttgcg ctcggcccag gtgaggatgc cctcgatcag ggtgccggac
      661 tcgttgtagc ggggctcgcc gacggtcagc accgcgtcca tgtggacatg cgggtcgacc
      721 agtgggggag agaggagcga gccctccgcg tcgagttcgg tggccgctga cgcgagttcc
      781 ccgcgctcgc gaatggcgcc gatccgccca tcctcgatgc cgatgtcgac cggttcggga
      841 tgcttcccgc cggtcgggtg cacgcgtgcg ttgcgaacga caaggtccaa ttgccacccg
      901 ctctccagag gggcgtcagc ggtgcgacgc gacgctgcat gatggagccg cagcgagcca
      961 gtctgcatgc tcttttcgca caggcaaatg tgaatctgcc ggctgccaat cggtcgccga
     1021 ttcgacgcag cgcacgcctc aatggcgggc gggactcgaa acgacacgcc gagtgacgaa
     1081 tcccgtcgaa atatgcggcc agtggcctgc cattgactgg tttcgcgtgg tagcaggtca
     1141 ttgcgagtcc atgccggcat agcgcctggt cagccgggtt ctagccatcc ctgcctcgga
     1201 ctgaccgtgc ttaccgatgc gatagactct ggcgcgggac ggggcgtagc gcagtccggt
     1261 agcgcacttg aatggggttc aagaggtcgc tggttcgaat ccagtcgccc cgaccactct
     1321 actgagcacg aaacggcccc tccggcattg tcaggagggg cctcttgttt ggtgtttggc
     1381 caccaccttg accactgctg gcccgtgctg aggatcccat ccaggcgctc gccagcccga
     1441 tagagcatcg ccggtgtgag agcgcggaca ggtcccccat gtgcgagtcg gtagcgcccc
     1501 ggcgtgggcc ggatgttcag ctcgttggac gggtgcagat ttcgccgcaa cgtcttgctc
     1561 ctgcccggcg tcggtttctt ggggacaaga aaccttgcag gccgcagagg agtgactgaa
     1621 cgtgaaccca ttcaaccgga gcgccctcac catattgagc ctgggcctgt tggtccttag
     1681 cccttcactc gcgagcgcag cgactgacag ggtcacggtt gagaacatgc gcgggcctct
     1741 cgtcaatgcc tggttctctt ccaccgatca gtctggctgc atcgagacgg acaccttcgt
     1801 caccgcccag cgcacgaccg accaactgct tcctggacgc ggcacgacgg ccggcatcgc
     1861 cgcggtcagc atctcccaat acgacgcgtg cacggatacg agcctgcttc aagcggtcgg
     1921 ccagacggac agcctcggag ccgcggagct ccagttctcc aatcaggtgg actgggcctc
     1981 gttgcacacc accatcacgg tcacgaatat cgaaacgggc gatgccttcg acgtcagcgt
     2041 gaacgttgcc tgggcgggca ccagcgacat cacgcgcgac cacagcaaca caaacgacct
     2101 ctacggcggc ggctgccacg tcctcaaccg ctggaagggc tctggccgca ccgcggacgc
     2161 gtcgggcctt gtctccgatg gcgcgaccaa cttcacgccc accacgtcac agtatgccga
     2221 gatcggcgtc gtgatcgacg gatttgaagt gattgactgc gcctgacctc ccggacagca
     2281 agacgcccga cccttgcggg ccgggcgctt gtcgaatcgc taccgggacg cttgcgcgcc
     2341 ccggcgggaa tcggtgctag gcggcagaga ccttgccggc gcggggaccg cgggggctgg
     2401 actcaacttc gaagttcacc ttctcgccac cgacgaggcg atcgaactca agggagcgat
     2461 cgagcccgtc acggtggaag aagtactcct tgccgtcctc ggccgcaatg aagccgaagc
     2521 cgcggtcgga tacgaccttc ttgatggtac ctgtggtcat gttgacctcg ttctcttctc
     2581 gaagtgagcc gacgacgcac ggccggctcg agaaggaacc tgacgaccac gcgggagact
     2641 cccaaggggg gagagtgcct ccagtctacc gtggatggcc gatctgtgct ccttgggcca
     2701 ctgtcgggga tgatcctggt ggcgcgccac ttccgtgcat tggagccgat gtagcatccc
     2761 gcggtggatc ccgcacccgc ccttcccgct gggccgccgt tcaccattcg agctcgcctg
     2821 ctcaccacgc tgggttccgg cgggctccgc tacgagcctg acgcccgggt cgaggtggat
     2881 ggcgcgggac ggcttgccag ggtcgagcgc tggtctgccg aggccgaccc aggcccgtcc
     2941 atcgacctgc gcccgtgggt catgatgccc ggcctgatcg acctgcatgc ccacctgcca
     3001 cagctgccga acgctggggt cggagccggg ctcgatcttc tgacctggct cgagcgatac
     3061 atctttccac tcgagcgcaa tttcgacgtc cccaccgcgg agctgatcgc gcctgccgcc
     3121 tttcgcgcct tcgcgcgggg cggcaccacc accgtggtcg cctatggcgc cctctgggcc
     3181 gacagcctgg acgcatgctt ccgcgctgcc gaggcgcatg gcatacgcgc cgtgatcggc
     3241 aaggtgatga tggatcggct cagctacgac acggaccacg atccgagcga gatgctcgag
     3301 ctgtcactgc gccagtccac ggacctgtgc gcgacatgga acgggcgaga tgacgggagg
     3361 ctcatgtacg ccttcacgcc acgcttcgcg gtgagctgct cggcggagat gctgcgcgcc
     3421 tccgccctgg ccgccaacga ggcaggcgcc tactggcaga cccatctctc ggaggaccgc
     3481 gaggagatct cggaggtccg gggcctcttc cccgatgcga tcgactacac cgatgtctac
     3541 gaccgcgccg gtgggctcac gccccgcgcg atcctggcgc acgccatcca cctgtcggac
     3601 cgggaggtgg cccgcctggc cgagagccgc gccgtcgtcg cccactgccc cagctcaaac
     3661 ctgttcctga cgagcggcgc gatgccgttg acgcgctacc tggcagcggg gatccgcatt
     3721 gggctcggct ccgatgttgc ggcgggaccc gacgtgcagc tcttcagcgt catgcgcgcc
     3781 ggcgcctaca cccagaacgc gctgcgggct gccggcctga gcgacgcacc acagttgagt
     3841 cccgccgact ggctgcgcct cggaacgctg ggtggcgccg aggcgctcgg gctgcaggac
     3901 cggatcggct cgatcgaggt gggaaaggag gcggatatga tcgttgtcga cccctctgtg
     3961 acgctaccgc tggagggggt tggcgcacac gatgatggag ggcccgacga gatgctcagc
     4021 cgtctcgtct tccgggcgca tccgtccatg gtccgcggcg cctgggtgcg tgggcgcctg
     4081 ctgcctgcct gacctgaccg gcttcggcta ggctagcggc catgggcatg gaccgggtca
     4141 tccagatcct catcaacgcc ggggcgctgt acgccgccga ttacctgatc agcggaatcc
     4201 ggttcagcgg cgaatggtgg aag
//
LOCUS       DATCQR010000212        13512 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_43122, whole genome shotgun sequence.
ACCESSION   DATCQR010000212 DATCQR010000000
VERSION     DATCQR010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 13512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13512
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43122"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(11..424)
                     /gene="sodX"
                     /locus_tag="VIN32_07045"
     CDS             complement(11..424)
                     /gene="sodX"
                     /locus_tag="VIN32_07045"
                     /inference="COORDINATES: protein motif:HMM:TIGR02754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0016485 - protein processing [Evidence
                     IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-type superoxide dismutase maturation
                     protease"
                     /protein_id="HEY8922366.1"
                     /translation="MARRQRLGGPLFLALGVAAGMTIGRRWLDVVEVRGGSMAPFLLP
                     GDRLLVESHTYQARAPRPGELVLAADPRTPERELIKRVFSVDDASSSAELRGDAPEAS
                     TDSRAFGAIPVSAIRWRAVFRYWPPRRVGRLAPSQ"
     gene            complement(429..860)
                     /gene="sodN"
                     /locus_tag="VIN32_07050"
     CDS             complement(429..860)
                     /gene="sodN"
                     /locus_tag="VIN32_07050"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012894678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase, Ni"
                     /protein_id="HEY8922367.1"
                     /translation="MLTIRSLLRPSRIVDAHCDIPCGVYDPEQARIEAESCLRIIEKH
                     DASEDKLFRARCIHVKEERAELVKHHLDVLWHDYFKPEHLEKYPDLHDTFWKATKQAS
                     KVKQSLDAAAAKELLAMIDTIDEMWKATGGPEKTRVNGRPS"
     gene            complement(946..1701)
                     /gene="fabG"
                     /locus_tag="VIN32_07055"
     CDS             complement(946..1701)
                     /gene="fabG"
                     /locus_tag="VIN32_07055"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019003700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEY8922368.1"
                     /translation="MYDLTDKVALVTGGSRGLGRAIALALARQGADVGVNYRGNAEAA
                     AEVVTEIRSMGRAAIAIQGDTSAGRESCEGIVKAAIDEFGKVDILVNNAGITRDNLLM
                     RMDADEWESVINTNLSGPFWMTRAIARPMLKAKSGRIINMSSVAGRMGNVGQANYASA
                     KAGLIGLTKSVARELASRGITCNAIAPGLIETELTADVPQAARDFVINSTPLGYIGSV
                     DDVAAAAVYFASDESRYVTGQVLGVDGGMMMGS"
     gene            complement(1701..2777)
                     /locus_tag="VIN32_07060"
     CDS             complement(1701..2777)
                     /locus_tag="VIN32_07060"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HEY8922369.1"
                     /translation="MALNAVITGWGMYAPSRVMTNEDLAKLVDTSDEWIESRTGIRER
                     RIAADDENTTSLSVRAARDALAVAGLDPSEVDLVIVATCSPDYPLPATSVTVATALGA
                     TRAAAFDLQAACSGFLYALATANGFIRSGMYRNVLVIGVEVLSRFLNWKDRSTCVLFG
                     DGAGAVLVSASEQPGGLMGWDLFSDGTGCEGIIVRAGGSVQPASAETVAGNLHTIQMA
                     GTEVYKYATRQMGDSAIAALRDAGLGVDDVDQFVFHQANLRIIKSVEKQFDIPEEKVF
                     VNIEKYGNTSAASVPMALVEAIAAGRIKPGDKVLMVAFGAGYTAGAAVMEWTADPARA
                     FLAPGEQTRLGMPQVVEPELAVAG"
     gene            complement(2803..3270)
                     /locus_tag="VIN32_07065"
     CDS             complement(2803..3270)
                     /locus_tag="VIN32_07065"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEY8922370.1"
                     /translation="MSADDAPSKQVLAAIDLLAPGFEASGLDELEVEAGELVVRLARP
                     RAPVAAPAATGSAPALDAAAGPLTPYGEPAPGMRFVTAPLTGVWYASPSPGARPYVQE
                     GDEIGAGAVVGLIEAMKLFNEIKSDVAGKVVRVLVERGTLVKRQQPIVEIDPR"
     gene            complement(3267..4556)
                     /gene="fabF"
                     /locus_tag="VIN32_07070"
     CDS             complement(3267..4556)
                     /gene="fabF"
                     /locus_tag="VIN32_07070"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017319976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEY8922371.1"
                     /translation="MTDRWQRPSSAERGERRVVVTGMGAITPIGNDVPSYWESLVAGR
                     SGIECITSFDPSRLDVQIAGEVKDFQPELVMSRKEVRRHDRYVHLAWAASVEALADAG
                     MPNPIVDDSLAEGTGVIIGSGIGGINTMIRDVIEAHDLGVERVGPFLVTSLIPDMGAG
                     FVAINANARGPNYATVSACSSSNHAIGDALNIIRRGDAEVMLAGGAESGIGEIPVAAF
                     AAMRALSTRNDAPQKASRPFDAERDGFIMADGAGILVLEALDHALARGARVHGELVGY
                     AATDDASHITLPAPGGRGAVASMSLAMADAGLKTDEIGYINAHGTSTVPNDKAETAAI
                     KTVFGEGAYQVPVSSTKSMTGHLLGAGGGIEAIACILAMKNGILPPTINYENPDPDCD
                     LDYVPNVARKVAIDTAMSNSFGFGGHNATLIFKRYVA"
     gene            complement(4553..5074)
                     /gene="fabZ"
                     /locus_tag="VIN32_07075"
     CDS             complement(4553..5074)
                     /gene="fabZ"
                     /locus_tag="VIN32_07075"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014829430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEY8922372.1"
                     /translation="MEDEAPMKDDAAATATAVDLALPLEAADIMRIIPHRYPFLLIDR
                     VIELEPGKRVVAIKNVTADEPQFTGHFPGRPIMPGVLMVEALAQAGAVAVLSLPEYAG
                     KLALFAGIDECRFRRTVLPGDQLVLKVELEKLRGMFGRARAVASVDGEVAVEATISFV
                     IPRDQSIAGGEPK"
     gene            complement(5080..6444)
                     /gene="accC"
                     /locus_tag="VIN32_07080"
     CDS             complement(5080..6444)
                     /gene="accC"
                     /locus_tag="VIN32_07080"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006521748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEY8922373.1"
                     /translation="MFSKIMIANRGEIAVRILRACRDLGVPAVVAFSEADRDTLAVRM
                     ADEAICIGPAEARKSYLNQPAVVSAAIIAGCDAVHPGYGFLSEDAGFAEVCAAHDLHF
                     IGPRADVLEKFGSKYAVRRMLAANGLPTVPGSTGILSDLRDALDQASAAGYPVLLKPS
                     AGGGGRGMRLVRSPREMETSLPLARSEAQAAFGDDSVYFEKWIEESRHVEVQVLIDQH
                     GNGIHLGERDCSVQRRHQKIIEETPSPAMSDDARERLRDLALRSVLAAGYENAGTLEF
                     LLDGDGNFYFIEINCRIQVEHPITEMLTGIDLIAEQIRLASGEKLALRQEQVQFRGHA
                     IEFRINAEDPGDNFAPQTGTVEELQLPGGPGVRVDTHLYAGYEVPPYYDSLLAKLIVW
                     GETREIALARSRRALAEFQVAGIKTNLPFHRGIIDNAAFLEGAVSTNLLDRVGPAAFV
                     AGSG"
     gene            complement(6444..7409)
                     /gene="fabD"
                     /locus_tag="VIN32_07085"
     CDS             complement(6444..7409)
                     /gene="fabD"
                     /locus_tag="VIN32_07085"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015255005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEY8922374.1"
                     /translation="MTVALLFSPQGSQSVGMGRELAVSSPAAAAVFREADEALGRAVS
                     TLAWEGPEDELNDTRQTQPCLVTTSIACLRALEEKLYGMGETVSPAFVAGHSVGEYAA
                     LVAAGALSLAGAVRLVARRGDLMADARVSGGMVAVIGLDREAVATIVSGLGLGADLVV
                     ANDNAPGQVVISGTPDALTAAAAPLRAAGARRSIPLNVSGPFHSPHMAGLGAELARAL
                     ATADWHDAEPPVVSNVSGEPVRDAAEIRDLLARQVHSPVEWVRSVQRMAADGVDTFVE
                     CGPGKALTGMVRRIVPAVHTLNVFDRASLAASADALVAAALRVPA"
     gene            complement(7406..7870)
                     /locus_tag="VIN32_07090"
     CDS             complement(7406..7870)
                     /locus_tag="VIN32_07090"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEY8922375.1"
                     /translation="MTDGEDPLQQIERAAAELIPQLTERLSQHGLGEIEVRHGDLRIR
                     VARAIAQPQAQTPGRAAPAHNALPSDRVGVVPSGPAAQGVTSPAVGFFVYADGLGPGL
                     PVDKGDELGHVEMLGVRHDVRAPRRGLVRNLVTETGEAVEYGQLLIEMEPAE"
     gene            complement(7867..8859)
                     /locus_tag="VIN32_07095"
     CDS             complement(7867..8859)
                     /locus_tag="VIN32_07095"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HEY8922376.1"
                     /translation="MSELLLSAEPPSAEAIESAWRRVQLARHPQRPRTLDLIALIFDD
                     FAELHGDRSFRDDPAIVGGPAMLDGRPVMVIGHQKGKDTESNIFRNFGSPYPEGFRKA
                     QRLMRLADKLGIPLVTFLDTAGAFPGPAAEERGQAEAIASSIKLMTGLQVPIVVVIIG
                     EGGSGGALAIGVGDEVLALENATYSVISPEGCAAILWRAPEAAPAAAAAMRMTAADQL
                     ELGVIDGVIEEPGEGAHADHAGTAAAVKLAVLGALGRITRLETEALLTSRYARLRGIG
                     LVRETEVTSAQLPQEPSLRRRIGRLLRLPGVPRRPRWSEIWPSGEDADSSEEGA"
     gene            complement(8856..9860)
                     /gene="accD"
                     /locus_tag="VIN32_07100"
     CDS             complement(8856..9860)
                     /gene="accD"
                     /locus_tag="VIN32_07100"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014683641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HEY8922377.1"
                     /translation="MQIKLPFSRRREVTPETWTVCPSCESQIFNRQLERNLRVCPTCG
                     HHFRMGIGQRIELLLDDGSFVEHDAGLESGDPLGFHDQKPYVDRLEAARIKTGLREAA
                     VWGIGTIEGQRVAIGLFDFHFMGGSMGSVVGEKLARCFESALLERIPAIVVSASGGAR
                     MQEGTLSLLQLAKTTAPLVRLDAAGIPFISILTDPTTGGVLASFASLGDVILAEPKAL
                     IGFAGARVASGTVGEELPEGFQSAESLLEHGFVDDIVPRSELRATLSRLLRLLPVAPA
                     EDGWQTTGDPRGWGPIGVLSGMAERLGTAVSETIGIEVEQPAADESGPGGRRAREEER
                     "
     gene            complement(9870..10133)
                     /locus_tag="VIN32_07105"
     CDS             complement(9870..10133)
                     /locus_tag="VIN32_07105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8922378.1"
                     /translation="MLRSALVPLPAGSRFEARLASRLAGHGSLERFVAGIGDATRREL
                     RHPARILLTGAVSSAAVGVGVTVLAMRRVARRHAATGHHPAHR"
     gene            complement(10308..10880)
                     /locus_tag="VIN32_07110"
     CDS             complement(10308..10880)
                     /locus_tag="VIN32_07110"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEY8922379.1"
                     /translation="MSEPSLRRNLAPAPDGDADRADVLAAQSDRAAFEVLYRRYLDRV
                     YGYAFYQLRDHHDAEDATERTFLAALKALPEYRDEGATFRAWLFRIAHNTIINARRSR
                     FRRHTEPLPDGLDRIAPNSDPAGLVVEADEARRVLRAVAELPDDRRQVVLLRFVDGLS
                     SREIGQVMDRSEGAARVLLHRALRDLADRL"
     gene            complement(10877..11689)
                     /locus_tag="VIN32_07115"
     CDS             complement(10877..11689)
                     /locus_tag="VIN32_07115"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEY8922380.1"
                     /translation="MTDAADWSRAASPARRIGQAVEFHAEVASTNDRARAALSEPGGE
                     GLVVVADLQTAGRGRRGRTWISPAGANLLFSVAWRPTIAPSLASLLGGSTALAVRDAC
                     AAFVPDAGLAIRWPNDVVDRNGRKVAGLLVETALESGELAEAVIGIGINVNWLPSEMP
                     PEIGGSATSLQALVGAPIDRVQLLDGVLNALDAEVRALERGDTPLPRLRASSWLDGRQ
                     VEVDTGSATITGRAAGIADDGSLLLDAESGRLALSVGEVARVHEAIPATVAQ"
     gene            11772..12773
                     /gene="trpS"
                     /locus_tag="VIN32_07120"
     CDS             11772..12773
                     /gene="trpS"
                     /locus_tag="VIN32_07120"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004095957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEY8922381.1"
                     /translation="MTETTVRERVFSGIQPSGASHLGNYLGAQANYVALQDQYETIYA
                     IVDYHAMTTVHDGQKMRELTHEMVLDLLAVGLDPERCALIRQSDFPEHAELAWIFNTV
                     APVSWVERTPTYKEKRDEGVENSMGLLDYPVLQAADIVIYKASRVPIGKDQAAHLELS
                     REIVRAFNRRYGPIFPEPQAVFTDAPVVLGTDGVRKMSKSAGNTIPIFAEPDEIRRIV
                     QKMVTDPQRIKRTDAGRPEVCNVCQLHRFFGEDYLQIQDGERTARTGCVETKELLAER
                     IIERFRPMRARRQELAADPGHVERVLAAGAAKVRPILEATMQEVRAAIGIGPPVGGG"
     gene            12773..>13512
                     /locus_tag="VIN32_07125"
     CDS             12773..>13512
                     /locus_tag="VIN32_07125"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEY8922382.1"
                     /translation="MSEERTTPQERNGGIGPRERRWLLAFLILGSAYFAVLLIEILLH
                     FLGGFSQILLILFLAWLLAFVMSPVVRFFEGLRISRPIAVAVAYLFALVTMGFVLFTT
                     GAAITQQLAQANDDFPHTAATIEATLTRYQSTLGLDRLSIDLVGLFHSAQDQVGILAG
                     QIFDQAQGIAGATLATLGSLFLILILSLYMLIDSERLLAKINRAVPRQYSDEMQILES
                     SVARAFGGFLRAQLILAVIQAVLVAIVG"
BASE COUNT         2032 a         4746 c         4632 g         2102 t
ORIGIN      
        1 gcctgagggc ttactgactc ggcgcgaggc ggcctacccg ccgcgggggc caatagcgga
       61 acacggcgcg ccagcggatc gccgacacgg gaattgcacc aaacgcgcga gagtcggtgc
      121 tggcctcggg agcatcgccg cgcagctcgg ctgacgacga ggcatcgtca accgagaaga
      181 cgcgcttgat gagctcgcgc tcgggcgtgc gtggatcggc agccaatacc agctcaccgg
      241 gacgcggcgc gcgcgcctgg taggtatgcg actcgaccag caggcgatcc ccggggagga
      301 ggaacggggc catcgagccg ccccggacct cgacgacatc gagccagcgg cggccgatcg
      361 tcatgccggc ggccacgccg agtgctagga agaggggacc gccgagcctc tgccggcggg
      421 ccatcggcct aggagggtcg gccgttgacg cgggtcttct cggggcctcc cgtcgccttc
      481 cacatctcgt cgatcgtgtc gatcatcgcc agcagctcct tcgctgcggc ggcgtcgagg
      541 gactgcttga ccttgctcgc ctgcttggtc gccttccaga aggtgtcgtg caggtcgggg
      601 tacttctcga ggtgctccgg cttgaagtag tcgtgccaca ggacgtcgag gtggtgcttc
      661 accagctcgg cacgctcctc cttcacatgg atgcagcgag cgcggaagag cttgtcctcg
      721 ctggcatcgt gcttctcgat gatgcgcagg caggactcgg cctctatgcg cgcctgttcg
      781 gggtcataga cgccgcacgg gatgtcgcag tgggcatcca cgatgcggga aggacgcagc
      841 agggaacgga tcgtcagcat caggctcctc atgtatcagt cttgggtgca aggagaagtt
      901 tagccagccg acgagcatgg gtcatttcgc cgacccgact gcgggtcagc tgcccatcat
      961 catgccgccg tcgacgccga gcacctgccc cgtgacgtag cgcgactcgt ccgatgcgaa
     1021 gtagaccgcg gcggcggcca cgtcgtcgac ggagccgatg tacccaagcg gcgtgctgtt
     1081 gatcacgaag tcgcgggcag cctgcgggac gtcggccgtc agctcggtct cgatcaggcc
     1141 gggggcgatg gcgttgcagg tgatcccgcg gctggccagc tcacgggcca ccgacttggt
     1201 gaggccgatc agccccgcct tggcgctcgc gtagttggcc tggccgacgt tccccatccg
     1261 gccggccacg ctgctcatgt tgatgatgcg gcccgacttc gccttgagca tcggcctggc
     1321 gatggcgcgc gtcatccaga acgggccgga caggttggtg ttgatgaccg actcccactc
     1381 gtcggcgtcc atccgcatca gcaggttgtc gcgcgtgatc cccgcgttgt tgaccaggat
     1441 gtcgaccttg ccgaactcgt cgatcgccgc cttgacgatc ccctcgcagg attcgcggcc
     1501 ggcgctggtg tcgccctgga tggcgatcgc ggcgcggccc atggaccgga tctcggtgac
     1561 cacctcggcc gctgcctcag cattgccgcg gtagttgacc ccgacgtcgg ccccctgcct
     1621 ggccagggcc agcgcgatgg cgcgaccgag cccgcggctc ccgccggtga ccagggcgac
     1681 cttgtcggtc aggtcgtaca tcagccggcg accgcgagct cgggctccac gacctgtggc
     1741 atgccaaggc gcgtctgctc gccgggtgcc aggaaggcac gcgccgggtc cgccgtccat
     1801 tccatcaccg ccgcgccggc cgtgtagccg gcgccgaagg cgaccatcag gaccttgtcg
     1861 ccaggcttga tgcgcccggc cgcgatcgcc tcgaccaggg ccatcggcac cgaagccgcc
     1921 gatgtgttgc cgtacttctc gatgttgacg aagaccttct cctcggggat gtcgaactgc
     1981 ttctccacgc tcttgatgat gcgcaggttg gcctggtgga agacgaactg gtcgacgtcg
     2041 tcgacgccca gccccgcatc tcgcagcgcc gcgatcgccg agtcgcccat ctggcgggtg
     2101 gcgtacttgt agacctcggt gcctgccatc tggatggtgt gcaggttgcc cgcaacggtc
     2161 tccgccgatg ccggctggac gctgccgccg gcccgtacga tgatgccctc gcagccggtg
     2221 ccatcgctga acaggtccca ccccatcagg ccgccgggct gctcggaggc ggacacgagc
     2281 accgcgccgg caccgtcgcc gaagaggacg caggtgctgc gatccttcca gttgaggaag
     2341 cggctcagca cctcgacccc gatcacgagc acgttgcgat acatcccgct ccggatgaag
     2401 ccgttcgccg tggcgagggc gtaaaggaac ccggagcagg ccgcctgcag gtcgaacgcc
     2461 gcggcccggg tggcgccgag cgcggtcgcc acggtgacgg aggtcgccgg cagcggatag
     2521 tcgggcgagc aggtcgccac gatcaccagg tccacctccg acggatccag gccggcgacg
     2581 gccagggcgt cacgcgcggc gcggacgctg aggctggtgg tgttctcgtc gtcggcggcg
     2641 atccgccgtt cgcggatccc ggtccgcgac tcgatccact cgtcggaggt gtcgacgagc
     2701 ttggcgagat cctcgttggt catcacgcgc gatggcgcat acatccccca ccctgtgatc
     2761 acggcgttca gcgccatcgg tcctccttct gcgatcgcat ggtcagcgcg ggtcgatctc
     2821 gacgattggc tgctgtcgct tcaccagcgt cccgcgctcg accaggacac ggacaacctt
     2881 gccggccacg tccgacttga tctcgttgaa cagcttcatt gcctcgatca ggccaaccac
     2941 cgcgccggca ccgatctcgt ccccctcctg gacgtacggg cgcgcgcccg gcgacggaga
     3001 ggcgtaccag acaccggtca gcggggcggt cacgaaccgc atccccggcg ccggctcacc
     3061 atacggggtg agcggaccag ctgcggcatc gagcgccggt gcgctgccgg ttgccgctgg
     3121 ggctgccacc ggcgctcgtg gccgggcgag tcggacgacc agctccccag cctccacctc
     3181 gagctcgtcg aggccgctcg cctcgaagcc gggcgccagc aggtcgatgg ctgccaggac
     3241 ctgcttcgag ggggcgtcat cggcgctcac gccacgtacc gcttgaagat gagcgtggcg
     3301 ttgtgcccgc cgaagccgaa cgagttgctc atggccgtgt cgatcgccac cttgcgtgcc
     3361 acgttcggga cgtagtcgag gtcgcagtcg gggtccgggt tctcgtagtt gatggtgggc
     3421 ggcaggatcc cgttcttcat ggccaggatg caggcgatcg cctcgatccc gcctccggcg
     3481 ccgagcaggt gcccggtcat gctcttggtg ctgctgaccg ggacctggta ggcgccctcg
     3541 ccgaagacgg tcttgatcgc cgcggtctcc gccttgtcgt tgggcaccgt gctggtgcca
     3601 tgagcgttga tgtagccgat ctcgtcggtc ttcaggcctg catcggccat ggccaggctc
     3661 atcgacgcga ctgcgcctcg accgccgggt gccgggagcg tgatgtggga tgcatcgtcg
     3721 gtggcggcgt agccgaccag ctctccgtgg acgcgagcgc ctctggcgag ggcgtgatcc
     3781 agcgcctcga ggacgagaat gccggcgcca tcggccatga tgaagccgtc gcgctcagcg
     3841 tcgaaggggc gcgaggcctt ctgaggtgcg tcgttgcgcg tcgagagggc gcgcatggcg
     3901 gcgaacgcgg cgaccggaat ctcgccgatg cccgattcgg cgccacccgc gagcatcacc
     3961 tccgcatccc cgcgacggat gatgttgagc gcgtcgccga tagcatggtt gctgctgctg
     4021 caggcgctga ccgtcgcgta gttcggcccg cgggcgttcg cgttgatggc cacgaacccc
     4081 gcgcccatgt ccgggatgag tgaggtcacc aggaacggtc cgacccgctc gaccccgagg
     4141 tcgtgcgcct cgatcacgtc gcggatcatg gtgttgatcc cgccgatccc cgaaccgatg
     4201 atgacaccgg tcccctcggc cagcgagtcg tccacgatcg ggttgggcat cccggcatct
     4261 gccaacgcct cgaccgaggc ggcccacgcg aggtggacgt agcgatcgtg ccgtcggacc
     4321 tctttcctgg acatcaccag ctcgggctgg aagtccttca cctcgccggc gatctgcacg
     4381 tcgaggcgcg aggggtcgaa ggaggtgatg cactcgatcc cgctgcgacc ggcgaccagc
     4441 gactcccagt acgacggcac gtcgttgccg atgggggtga tggctcccat gccggtcacc
     4501 accacgcgcc gctcgccgcg ctccgcggag ctgggtctct gccagcggtc ggtcatttgg
     4561 gctcgccccc cgcgatcgac tggtcgcgcg gaatcacgaa gctgatggtc gcctcgacgg
     4621 ctacttcgcc gtccacgctg gccaccgcgc gtgcccgtcc gaacatgccg cgcagcttct
     4681 cgagctcgac cttgaggacg agctggtccc ctggcaggac ggtgcgccgg aagcggcact
     4741 cgtcgatccc ggcaaacagc gccagcttgc cggcgtactc gggaagggaa agcacggcca
     4801 ctgctccagc ctgggccagc gcctcgacca tcaggacacc gggcatgatc gggcggcccg
     4861 ggaaatggcc ggtgaactgc ggctcgtcgg cggtcacatt cttgatcgca accacgcgct
     4921 tgccgggctc gagctcgatc acccggtcga tgaggaggaa cgggtaacgg tgcgggatga
     4981 tccgcatgat gtcggcagct tcgagcggca gcgcgaggtc gactgcagtc gcggtggcgg
     5041 cagcgtcgtc cttcatcggc gcttcgtcct ccatttctgt catccgctgc ccgccacgaa
     5101 ggcggcggga ccgacccggt cgagcaggtt cgtgctcacc gcgccctcca ggaaggctgc
     5161 gttgtcgatg atcccacgat ggaaggggag gttcgtcttg atccccgcca cctggaactc
     5221 ggcaagggcc ctccgtgagc gagccagggc gatctcgcgg gtctcgcccc acacgatcag
     5281 cttggccagc agcgagtcgt agtagggcgg aacctcgtag ccggcgtaga ggtgcgtgtc
     5341 gacgcggacc ccggggccgc ccggtagctg cagctcctcg accgtccccg tctgcggggc
     5401 gaagttgtcg ccgggatcct cggcgttgat gcggaactcg atggcatggc ccctgaactg
     5461 gacctgctcc tgccgcagcg ccagcttctc gcctgacgcc agccggatct gctccgcgat
     5521 caggtcgatg ccggtcagca tctcggtgat gggatgctcg acctggatgc ggcagttgat
     5581 ctcgatgaag tagaagttgc cgtcgccatc cagcaggaac tcgagggtgc cggcattctc
     5641 gtatccggcc gccagcaccg agcggagggc gaggtcacgc agccgctccc tcgcgtcgtc
     5701 gctcatcgcg ggcgagggcg tctcctcgat gatcttctgg tgacgccgct gcacgctgca
     5761 gtcgcgctcc ccaaggtgaa tcccgttgcc atgctggtcg atgagcacct ggacctcgac
     5821 gtgccggctc tcctcgatcc acttctcgaa gtacaccgag tcgtcgccga aggccgcctg
     5881 cgcctccgac cgggccagcg gcagggaggt ctccatctcg cgcggtgaac gcaccaggcg
     5941 catgccccga ccgccgcccc cggccgacgg cttgagcagc accggatagc cggccgcgga
     6001 tgcctggtcg agcgcatcgc gcaggtcgct caggatcccg gtgctgcccg gcaccgtggg
     6061 gaggccgttg gcggccagca tgcgtcgcac cgcgtacttg ctgccgaact tctcgagcac
     6121 gtccgctcga gggccgatga agtgcaggtc gtgcgccgcg cacacttccg cgaagccggc
     6181 atcctccgag aggaacccgt agccggggtg gacagcgtcg cacccggcga tgatcgcggc
     6241 ggagacgacg gctggctgat taaggtagct cttgcgagct tcagccggac cgatgcagat
     6301 cgcctcgtcg gccatgcgca cggcaagcgt gtcacgatcc gcctcgctga acgcgaccac
     6361 ggccggcacc cccaggtcgc ggcaggcgcg caggatgcgc acggcgatct cgccccggtt
     6421 ggcgatcatg atcttgctga acatcaggcc ggcactcgta gcgccgccgc gaccagcgca
     6481 tccgccgacg ccgccaggct ggcgcgatcg aatacgttca gcgtgtgcac cgccggcacg
     6541 atccgacgca ccatgcccgt cagggccttg cccggaccgc attcaacgaa ggtgtcgacc
     6601 ccgtcggcag ccatgcgttg caccgacctg acccactcga ccggcgagtg cacctggcgg
     6661 gcgagcaggt cccggatctc ggcggcgtcg cgcaccggct cgccgctgac gttgctgacc
     6721 accggtggct cggcatcgtg ccagtccgcc gtcgccaggg cgcgcgccag ctctgcgccg
     6781 agacccgcca tgtgcgggga atggaaaggc ccggacacgt tgagtggaat ggaccgccga
     6841 gctccggccg cacgcaaggg tgcggcggca gcggtcagcg cgtccggcgt gccgctgatg
     6901 acgacctggc ccggggcatt gtcgttggcg accacgaggt ctgcccccag ccccaggccc
     6961 gagacgatgg tcgcgaccgc ctcccgatcc aggccgatca ccgccaccat gccgccgctc
     7021 acccgcgcat cggccatcag gtcgccgcgt cgcgcgacga ggcgcacggc gccggccagg
     7081 gagagcgcgc cggccgcgac cagcgctgcg tactcgccca ccgaatgacc cgcgacgaag
     7141 gccggggaga ccgtctcgcc catcccgtac agcttctctt cgagggcgcg gaggcaggcg
     7201 atcgaggtcg tcacgaggca gggctgggtc tgcctcgtgt cgttcagctc gtcttccggt
     7261 ccctcccatg cgagggtcga aacggccctg ccgagcgcct cgtcagcctc gcgaaagacc
     7321 gccgccgccg ccggagagct gaccgccagc tcgcgtccca tcccgacgga ctgcgacccc
     7381 tgcggcgaaa agaggagcgc gacggtcact cggcaggttc catctcgatc aggagctggc
     7441 catattcgac tgcttccccg gtctccgtga cgaggttgcg gacgaggccc cggcgcggcg
     7501 cgcgaacgtc gtgccggacg ccgagcatct cgacgtggcc cagctcatcc cccttgtcga
     7561 ccgggagccc aggccccagc ccatcggcat acacgaagaa gccgacggcg ggcgacgtca
     7621 cgccctgggc cgccgggccg ctgggcacga cgccgacccg gtcgctcggc agcgcattgt
     7681 gcgcgggcgc ggcacgaccg ggagtctgcg cctggggctg ggcaatggcg cgcgcaacgc
     7741 ggatgcgcag gtcgccgtgg cgcacctcga tctcgccgag gccgtgctgg ctcagccgct
     7801 cggtgagctg tggaatcagc tcggccgcgg ctcgctcgat ctgctggagc gggtcctctc
     7861 cgtcggtcat gcaccctcct cgctgctatc ggcatcctca ccgctcggcc agatctcgct
     7921 ccagcgcggc cggcgcggaa cccccggcaa tcgcagcagc cggccgatcc ggcggcgaag
     7981 ggatggctcc tgcggcagct gcgccgaggt gacctccgtc tcccggacca gcccgatccc
     8041 gcgcagacga gcatagcggc tcgtcaggag ggcctcggtt tcgagcctgg tgatcctgcc
     8101 caacgccccc aggacggcca gcttgaccgc cgcggcagtg cccgcatggt cggcatgggc
     8161 gccctcgccg ggctcctcga tcacgccgtc gatcacgccc agctcgagct ggtcggccgc
     8221 ggtcatgcgc attgccgcag cggccgctgg tgccgcctcg ggggcgcgcc acaggatggc
     8281 tgcgcagccc tccggcgaga tcaccgagta cgtggcgttc tccagcgcga gcacctcgtc
     8341 tcccacgccg atcgccagcg cgccgccaga cccaccctcg ccgatgatga ccaccacgat
     8401 cggcacctgg aggccggtca tgagcttgat cgacgaggcg atcgcctcag cctgtccgcg
     8461 ctcctcggcc gcggggccgg ggaaggcacc ggccgtgtcg aggaaggtga cgagcgggat
     8521 ccccagcttg tccgccaggc gcatcaggcg ctgggccttg cggaacccct cggggtatgg
     8581 cgagccgaag ttgcggaaga tgttggattc ggtgtccttt cccttctggt ggccgatcac
     8641 catcaccggc cgaccgtcga gcatcgcggg cccgcccacg atcgcaggat cgtcgcgaaa
     8701 ggatcggtcg ccgtgcagct cggcgaagtc gtcaaagatg agcgcgatca ggtccagggt
     8761 ccgtggccgc tgcgggtggc gggccagctg gacgcggcgc caggccgact cgatcgcctc
     8821 agcggacggc ggttcggcgc tgagcaagag ctcgctcatc gctcctcctc gcgagctcgg
     8881 cggccgccgg ggccggattc atcggcggcc ggctgctcga cctcgattcc gatcgtctcg
     8941 ctgacggccg tccccaggcg ctccgccatg cccgagagca ccccgatcgg cccccagccc
     9001 cgcggatcgc cggtcgtctg ccagccatcc tcggcaggtg caaccggcag gaggcgcagc
     9061 aggcgcgaca gagtggcccg cagctcggag cgcggcacga tgtcgtcgac gaacccgtgc
     9121 tcgagcagtg attccgcgct ctggaagccc tccggcagct cctcccccac cgtgccgctc
     9181 gccacgcgag ccccggcgaa gccgatgagc gccttcggct cggccaggat cacgtcgccc
     9241 agcgaggcga agctggccag cacgccgcct gtggtcgggt cagtgaggat gctgatgaac
     9301 gggattccgg cagcgtcgag gcgaacgagc ggggcggtcg tcttggcgag ctgcaggagc
     9361 gagagggtcc cctcctgcat gcgcgcgccg cccgaggcgc tgaccacgat ggcggggatc
     9421 cgctcgagga gggcagattc gaagcagcga gccagcttct cgccgaccac gctgcccatg
     9481 ctgccgccca tgaagtggaa gtcgaagagg ccgatcgcga cccgctgccc ctcgatggtg
     9541 ccgattcccc acaccgccgc ctcgcgcagc ccggtcttga tgcgcgccgc ctcgaggcga
     9601 tccacgtagg gcttctggtc gtggaagccg agggggtcgc ccgactccag gccggcgtca
     9661 tgctcgacga agctcccgtc gtccagcagc agctcgatgc gctgcccgat ccccatccgg
     9721 aagtggtgcc cacaggtcgg gcagacgcgc aggttgcgct ccagctggcg attgaagatc
     9781 tgcgactcgc agctgggaca cacggtccat gtttcgggcg tcacctcgcg gcgacgagag
     9841 aacgggagct tgatctgcat ctcgcctaac taccggtgcg ccgggtggtg gccggtggcg
     9901 gcgtggcgcc gtgccacgcg gcgcatggcg aggacggtga cccccacccc aacggcggcc
     9961 gacgagacgg cgccggtgag caggatccgc gcggggtggc gcagttcgcg ccgggtcgcg
    10021 tcgccaatgc ccgcgacgaa acgctcaagc gacccatggc ccgccagcct cgaggcgaga
    10081 cgggcctcga agcgggaccc ggccggaagc gggacgagcg ccgatcgcag catcgaggcg
    10141 gcgtcgacca gcggcgccag ctccggccca accgcgagat ggcgtccggt cagaaggtta
    10201 tcgacggcct catccaggcg ttcggccggc gtgcgggttg gcaggctggg tctcctcgat
    10261 cggtgcgggc attccccatc ctagccaaca ccgccggacg actttcgtta caggcgatcc
    10321 gccaggtcgc gcagggcgcg gtgcaggagc accctggcgg cgccctcgga tcggtccatc
    10381 acctgcccga tctcgcgcga gctgagccca tcgacgaagc gcagcaggac gacctgcctg
    10441 cgatcgtcgg gaagctcggc aacggcccgc aggactcgac gcgcctcatc ggcctccacg
    10501 accagtccgg cgggatccga gttcggggca atccgatcga ggccgtcggg gagcggctcg
    10561 gtgtggcggc ggaaccggct gcgtcgggcg ttgatgatcg tgttgtgggc gatcctgaag
    10621 agccaggctc ggaaggtcgc cccctcgtcg cggtactccg gcagggcttt cagcgcggcc
    10681 aggaaggttc gctctgttgc gtcctcggcg tcgtggtggt cgcgcagctg gtagaaggcg
    10741 tagccgtaca cgcgatccag gtagcgccgg tagagcacct cgaaggcggc gcggtcgctc
    10801 tgcgccgcca gcacgtcggc gcggtcggcg tcaccgtccg gggccggcgc gaggtttcgt
    10861 cggaggctcg gttcgctcat tgcgccaccg tggccggaat cgcctcgtgc acccgcgcca
    10921 cctcgccgac gctgagggcg agccgtccgg actcggcatc gaggaggagt gagccgtcgt
    10981 ccgcgatgcc ggcggcgcgc ccggtgatgg tggcgctgcc ggtgtcgacc tcgacctgtc
    11041 gcccatcgag ccacgagctg gctcgcaggc gtggcagggg cgtgtcacct cgctcgaggg
    11101 cccgcacttc ggcgtcgagg gcgttgagca ccccgtcgag caactggacg cgatcgatcg
    11161 gtgcgcccac cagcgcctgc agcgaggtcg cgctgccccc gatctctggc ggcatctcgc
    11221 tcggcagcca gttgacgttg atcccgatcc cgatcaccgc ctcggccagc tccccgctct
    11281 cgagagccgt ctcgaccagc aggcccgcca ccttgcgacc attgcggtcg acgacgtcgt
    11341 tcggccaccg gatggcgagc ccggcatcgg gcacgaacgc ggcgcacgca tcgcggaccg
    11401 cgagcgctgt cgagccgccg aggaggctgg ccagcgacgg cgcgatggtg ggccgccacg
    11461 cgacgctgaa caggagattc gcaccggcag gcgagatcca ggtccggcca cggcggccac
    11521 gaccggcggt ctgcaggtcc gccacaacca cgagtccctc gccaccgggc tcggaaaggg
    11581 cggcccgggc gcgatcgttg gtcgatgcga cctctgcgtg gaactcgacc gcctgcccga
    11641 tcctgcgggc aggactggcg gcgcgcgacc agtcggctgc gtcggtcatt gcgccgcatt
    11701 atgccgcctc gggtcgaacg accgccccgg aggggcccag cccagcgcgt gcacgctaca
    11761 ctcgccgtcc gatgaccgag acgaccgtcc gcgagcgtgt cttcagcgga atccagccgt
    11821 cgggggcctc gcacctgggc aactacctcg gcgcgcaggc caactatgtc gcgctccagg
    11881 accagtacga aacgatctac gcgatcgtcg actaccacgc catgaccacc gtccacgacg
    11941 gacagaagat gcgcgagctg acccacgaga tggtgctcga cctcctcgcc gtgggactcg
    12001 acccggagcg ctgcgccctg atccgccagt cggactttcc cgaacacgcc gagctggcct
    12061 ggatcttcaa caccgtcgcg ccggtgagct gggtcgagcg gacccccacc tacaaggaga
    12121 agcgcgacga gggcgtcgag aacagcatgg ggctgcttga ctaccccgtc ctgcaggccg
    12181 ccgacatcgt catctacaag gccagccggg tgccgatcgg caaggaccag gcggcgcacc
    12241 tggagctgtc gcgcgagatc gttcgggcgt tcaaccgccg ctatggaccg atcttccccg
    12301 agcctcaggc ggtattcacc gacgcaccgg tggtgctcgg cacggatggc gttcgcaaga
    12361 tgagcaagtc ggccggcaac accatcccga ttttcgccga gccagacgag atccggcgga
    12421 tcgtgcagaa gatggtcacc gacccgcagc gcatcaagcg caccgatgcg ggccgtccag
    12481 aggtctgcaa tgtctgtcaa ttgcaccgct tcttcggcga ggactacctg cagatccagg
    12541 atggcgagcg caccgcgcga accggctgcg tcgagaccaa ggagctgctg gcggagcgca
    12601 tcatcgagcg cttccgccct atgcgggcgc gtcgccagga gctggcagcg gatcccggcc
    12661 acgtcgagcg cgtcctcgcc gcaggggcag ccaaggtccg tcccatcctg gaagcgacga
    12721 tgcaagaggt ccgtgccgcg atcggcatcg gccctcccgt gggaggaggc tgatgagcga
    12781 ggagcggacg acgccgcagg agcgaaacgg tgggatcggc ccccgcgagc gccgctggct
    12841 gctggcgttc ctgatcctgg gctcggcgta cttcgcggtg ctgctgatcg agatcctgct
    12901 gcacttcctg ggcggcttca gccagatcct gctgatcctg ttcctggcct ggctgctcgc
    12961 cttcgtgatg tcgccggtgg tccgcttctt cgagggcttg cggatctcgc ggcccatcgc
    13021 ggtcgcagtc gcctacctct tcgcgctggt caccatgggg ttcgtgcttt tcacgacggg
    13081 agcggcgatc acccagcagc tggcccaggc gaacgatgac ttcccgcaca cggcggccac
    13141 catcgaagcg acgctgaccc gctaccagtc aacccttggc ctcgatcgcc tgtcgatcga
    13201 cctggtcgga ctcttccaca gcgcgcagga ccaggtcggc atcctggccg ggcagatctt
    13261 cgaccaggcg caggggattg cgggggccac cctggcgacg cttggctccc tcttcctgat
    13321 cctgatcctg tcgctctaca tgctgatcga cagcgagcgc ctgctggcca agatcaaccg
    13381 cgcggtgccg cgacagtact ccgacgagat gcagatcctg gagagcagcg ttgcgcgcgc
    13441 ctttggcggc ttcctgcggg ctcagctgat tctggcggtg atccaggcgg tcctggtcgc
    13501 gatcgtcggc ag
//
LOCUS       DATCQR010000213         6244 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_12098, whole genome shotgun sequence.
ACCESSION   DATCQR010000213 DATCQR010000000
VERSION     DATCQR010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6244
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12098"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(24..1298)
                     /gene="purD"
                     /locus_tag="VIN32_07130"
     CDS             complement(24..1298)
                     /gene="purD"
                     /locus_tag="VIN32_07130"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018217915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEY8922383.1"
                     /translation="MRVLVLGGGGREHALAWRLSRDESVERVWIAPGNGGTAGVAASI
                     PDLPIADPATVARHAARERYDLVVIGPEAPLAAGVADALERASIPAFGPTRGAARLEW
                     SKVFAKEQLRLAGVATAASAVFDDVESAVAHAHASDRPPVVKADWPATGKGVIVPDTA
                     QAAEAAIRELLRDVPPGAQLLLEEQLAGREVSAFALVSGEMVVPLGAACDYKRLLDGD
                     QGPNTGGMGAYAPVPWFGAGQMEAAVASVFEPIAWRLARDGTPYRGILYAGLMLTDAG
                     PMVLEFNARFGDPEAQVLLPLLDGDLAAALLGVATGDRGSMEGSLALKQGASVGVVVA
                     AEGYPSEPVTGRRIEGAEPAAADDGGPLLCFHAGTSPMADGSHASKGGRVVTFVGLDG
                     DLADAREIAYRGVASCQLEGGQHRTDIALREL"
     gene            complement(1298..4225)
                     /locus_tag="VIN32_07135"
     CDS             complement(1298..4225)
                     /locus_tag="VIN32_07135"
                     /inference="COORDINATES: protein motif:HMM:NF015644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HEY8922384.1"
                     /translation="MSSWFDKLLEELQRRQTEQDARREGRPIPPFGPRRGAGNGHPDE
                     HGDDQPPPLRQRVLRPRDPGRQPRWGLIVGAILVLLLVLGVLGGLINLVTDLMWYDAL
                     GLRSVLTTRLWSQVALFAVGFVAFALPALASIWLARRIAPQVPIRRIGGLELPDVSRA
                     VTLALGGVVLLLALISAAAWSSSWQTVLLFINGGSFGSADPNFGHDIGFYIFDLPFWR
                     FLQGWGVTSLVVIILLTLGTYAVGALRWQLRLTAPVRAHLSILGALLLVAIATGYQFD
                     IAELSYSTRGVSGTIQAATYADIHAQVPAYVILTVVALASAALLLANIWFKTLWAIAL
                     AAGAWIGITILVGGVYPGIVQRLNVDPNELSLEQPYIQQHIKMTRAAYALDAIDVQPY
                     SGEQALSRQLITANQATIDNMRLWDWHPLLQTFGQQQILRQYYEFRDVDIDRYVIDGQ
                     QRQIMLSAREIKGPPEDAQSWTNERLAYTHGYGITAVPVNAVTPEGQPDYLVSGINQE
                     PKLPVGEPRIYFGEEPSSYVVVDTKASEFDYPLDDQNNAVTTWKGSNGVGVGNVASRI
                     LFAMRFGDLNLLISNQLTGDSQILFRREIGERVRELAPFLVYDKDPYLVSADGRLEWI
                     WDAYTVSDRYPDAQPLADIFPGQNYVRNSVKVVIDAYDGSVRFFISDPSDPIINAYAR
                     IFPDLLKPLDAMPATLRAHLRYPEDLFTAQNEAYLLYHLDATPQGAAALYNRADRWSI
                     PTQQTGVEGPGTPIAPHYVIMKLPAETKSEFVMVQPMVAAARPNMISWIAARMDPGHY
                     GERISFRFPSNSTTLGPSQIQARINQDSTISAQFTLWSRAGSEVVRGDLLVLPMGDSI
                     LYVEPIFLRSTESSFPEFKRVILASQNKLAFADSLAQGISQLLGEATIPPPDGGGGQL
                     PGDVASLVADAQRLYDAAQVALAAGDLGTYQARIDDLARVIEALGKLVGAPSPSPSAA
                     PSG"
     gene            complement(4286..5812)
                     /gene="guaA"
                     /locus_tag="VIN32_07140"
     CDS             complement(4286..5812)
                     /gene="guaA"
                     /locus_tag="VIN32_07140"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEY8922385.1"
                     /translation="MVILDFGSQFAQLIARRVRELNVYSELLPHDTPWAEIERRRPKA
                     IILSGGPSSVYDDGAPHPDPAIWAGGIPVLGICYGLQLMAHQLGGEVTPSTRREYGPA
                     SVQITTEDGLFRGLERTQPVWMSHGDRIVRPPTGFITTAETETSAFAGLADPTRGLYG
                     IQFHPEVVHTPAGRDILRNFVVGIAGARQTWTPASFVETTVAGIRERVGDGKVICALS
                     GGVDSAVAARLVHQAIGDQLTCIYVDHGLMRKRESELLRATFAENLGMKLVMVDARER
                     FLRRLAGVEDPEEKRRIIGDEFIRVFEEEAAKLGQIDFLTQGTLYPDVIESKTAETKT
                     AAKIKTHHNVGGLPADLRFSLIEPLRYLFKDEVRNVGVELGLPEAMVLRQPFPGPGLA
                     IRIIGEVTADRLDTLREADWIVIDEIKAAGLYRSLWQSFAILTPVRSVGVMGDGRTYA
                     NVVAVRAVTSEDGMTADWARLPYDLLARISSRIVNEVSGVNRVVYDISSKPPATIEWE
                     "
     gene            complement(6011..>6244)
                     /locus_tag="VIN32_07145"
     CDS             complement(6011..>6244)
                     /locus_tag="VIN32_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015406932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GuaB3 family IMP dehydrogenase-related protein"
                     /protein_id="HEY8922386.1"
                     /translation="PTLPRGTRIKIGERLPLEQILFGPAHTTHGTQNLVGALRQSMAS
                     LGARTIREMHRVEMVIAPSIATEGKSWQLAARE"
BASE COUNT          974 a         2147 c         2154 g          969 t
ORIGIN      
        1 atggcggctc tcaagcccgc cggttagagc tcgcggaggg cgatgtcggt tcgatgctga
       61 ccgccctcga gctgacagga ggcgactccg cggtatgcga tctctcgtgc gtcggcaagg
      121 tcgccgtcca ggccaacgaa ggtgaccacc cgcccgccct tcgatgcatg cgaaccatcc
      181 gccatggggc tcgtgccggc gtggaagcac agcagcggac cgccatcgtc agccgcggcg
      241 ggctccgcac cctcgatgcg ccgccctgtg accggctctg acggataacc ctcggcggcc
      301 accaccacac cgaccgatgc gccctgtttc agcgccagcg agccctccat cgacccgcgg
      361 tcgccggtcg ccacgccgag cagagcggcg gccaggtctc cgtcgagcag gggcagcagg
      421 acctgcgcct ctgggtcccc gaagcgcgcg ttgaattcga gcaccatcgg tcctgcgtcg
      481 gtcagcatca gtccggcgta gaggatcccg cgatacggcg tcccatcgcg cgccaggcgc
      541 cacgcaatcg gctcgaagac cgacgcgacc gcggcctcca tctggcccgc gccgaaccac
      601 gggaccggcg cataggcgcc catcccgcca gtgttcggcc cctggtcacc gtcgagcagc
      661 cgcttgtagt cgcaggctgc gccgagcggc accaccatct cgccgctgac gagggcgaag
      721 gccgacacct cccgtccggc cagttgctcc tcgagcagca gctgggcgcc cggcggcacg
      781 tcgcgcagca gctcccggat cgccgcctcg gccgcctggg ccgtatcggg cacgatcacg
      841 cccttgcccg tggccggcca atcggccttg acgaccggcg gtcggtcgct cgcgtgagca
      901 tgagcaactg cgctctcaac gtcgtcgaag acggcgctgg ccgcggtggc cacgccagcc
      961 agccgcagct gctccttggc aaagaccttg ctccactcga gccgggccgc ccctcttgtc
     1021 ggcccgaagg cgggaatcga ggcacgctcc agggcatcgg ccacgcccgc cgccagcggc
     1081 gcctcgggcc cgatcacgac gaggtcgtat cgttcgcggg ccgcatgccg cgcgacggtc
     1141 gccgggtcag caatcgggag atcggggatg gaggccgcca cccccgcggt gccgccgttg
     1201 cccggagcga tccagacccg ctcgaccgat tcgtcgcgcg acaggcgcca ggcgagtgcg
     1261 tgctcgcgcc cgcccccgcc caggaccagg acccgcatca gccgctgggc gcggctgaag
     1321 gagacgggga cggagcgccg acgagcttcc cgagggcttc gatgacacgt gcgaggtcgt
     1381 caatgcgcgc ctggtaggtg ccgaggtcgc ccgcggccaa cgccacctgg gcggcgtcat
     1441 agaggcgctg cgcgtccgcc accagcgagg ccacgtcgcc aggcagctgg ccgcctcccc
     1501 cgtccggcgg gggaatcgtc gcctcgccga gcagctggct gatcccctgc gcgagtgaat
     1561 cggcgaaggc gagcttgttc tggctggcca ggatcacacg cttgaactcg ggaaaggacg
     1621 actcggtgga gcgcaggaag atcggctcga cgtagaggat cgagtcgccc atcggcagga
     1681 ccagcaggtc accgcgaacg acctccgagc cggccctcga ccaaagggtg aactgcgcgc
     1741 tgatcgtcga gtcctggttg atccgggcct ggatctgcga tggacccaag gtcgtcgagt
     1801 tcgaggggaa gcggaagctg atccgctcgc cgtaatggcc cgggtccatc ctggctgcga
     1861 tccagctgat catgttcggc ctcgcggccg cgaccatcgg ctggaccatg acgaactctg
     1921 acttcgtctc ggccggcagc ttcatgatca cgtagtgcgg cgcgattggg gtgcccggcc
     1981 cctcgacacc agtctgttgg gtggggatcg accagcggtc agctctgttg tagagcgccg
     2041 ccgccccctg gggcgtcgcg tcgaggtggt agagcaggta ggcctcgttc tgcgcggtga
     2101 agagatcctc ggggtagcgc agatgtgcgc gcagggtcgc cggcatcgcg tcgagcggct
     2161 tgagcaggtc cgggaagatg cgcgcgtagg cattgatgat cgggtcgctc ggatcgctga
     2221 tgaagaagcg aaccgatccg tcgtaggcgt cgatgacgac cttcaccgag ttccgcacgt
     2281 agttctggcc agggaagatg tccgctaacg gctgcgcatc cgggtaccga tccgaaaccg
     2341 tgtaggcgtc ccagatccac tccaggcgtc catcggcgct gaccaggtat gggtccttgt
     2401 catagaccag gaagggagcc agctcccgca cccgctcccc gatctcgcgc cggaagagga
     2461 tctgcgaatc gccggtcagc tggttgctga tgagcaggtt gaggtcgccg aagcgcatgg
     2521 cgaagaggat gcggctggcc acgttgccga ccccgacccc gttgctgccc ttccacgtgg
     2581 tgacggcatt gttctggtcg tcgagcggat agtcgaattc gctcgccttg gtgtcgacca
     2641 cgacgtacga ggatggctcc tcgccgaagt agatccgcgg ctcgccgacc ggcagcttcg
     2701 gctcctggtt gatgccgctc acgaggtagt cgggctggcc ctccggcgtg accgcgttga
     2761 ccggcactgc cgtgatcccg tacccatgcg tgtacgccag ccgctcgttc gtccaggact
     2821 gcgcatcctc gggtggcccc ttgatctccc gcgcgctgag catgatctga cgctgctgcc
     2881 cgtcgatgac gtagcggtcg atatcaacgt cgcggaattc gtagtactgg cgcaggatct
     2941 gctgctggcc gaaggtctgg agcagcgggt gccagtccca gagccgcatg ttgtcgatcg
     3001 tcgcctggtt cgccgtgatc agctgccggc tgagcgcctg ctccccgctg tacggctgga
     3061 cgtcgatggc gtcgagcgca taggcggctc gggtcatctt gatgtgctgc tggatgtacg
     3121 gctgctcgag cgagagctcg ttcggatcga cattcagccg ctgcacgatg ccggggtaga
     3181 cgccaccgac caggatcgtg atcccgatcc acgccccggc ggccagcgcg atcgcccaca
     3241 gggtcttgaa ccagatgttt gccagcagca gcgcagcgga ggcgagcgcc accacggtga
     3301 ggatcacgta ggccggcacc tgcgcgtgga tgtcggcgta ggttgcggcc tggatcgtcc
     3361 cgctcacgcc acgggtcgag tacgacagct cggcgatgtc gaactggtag ccggtcgcga
     3421 tcgccaccag caagagcgcc cccaggatcg agagatgggc ccgcaccggc gccgtcaggc
     3481 gcagctgcca gcgcagcgca cccaccgcgt acgtccccag ggtgagcagg atgatcacca
     3541 ccagagaggt cacgccccag ccctggagga agcgccagaa gggcaggtcg aagatgtaaa
     3601 agccgatgtc gtggccgaag ttggggtcgg ccgagccgaa actgccgcca ttgatgaaga
     3661 ggagcaccgt ctgccagctg ctgctccagg ccgccgcgga gatgagcgcg agcagcagca
     3721 caacgccacc cagcgccagc gtcacagccc ggctgacatc cggcagctcg agcccgccga
     3781 tgcgacgaat cgggacctgc ggggcgatgc ggcgagcgag ccagatgctc gccagcgccg
     3841 gcagcgcgaa cgccacgaag cccacggcga agagcgcaac ctgcgaccag agccgcgtgg
     3901 tgagcacgct gcggaggcca agggcgtcgt accacatcag gtcggtgacc aggttgatca
     3961 ggccaccgag cactccgagg accaggagca gcacgaggat cgcgccgacg atgaggcccc
     4021 agcgcggctg ccgtcccggg tcgcgcgggc gcagcacccg ttgtctcaag ggaggcggct
     4081 ggtcgtcgcc gtgctcgtca gggtggccgt tgccggcgcc ccgccgcggt ccaaagggtg
     4141 ggatcggccg accttcgcgg cgggcgtcct gctcagtctg gcggcgctgc agctcctcga
     4201 gaagcttgtc gaaccagctg gacatgtgcg tcgcatccta gcggtgtgga cccgcgaccg
     4261 tatccgggag cccagctagc tgggatcact cccactcgat ggtcgccggg ggcttgctgc
     4321 tgatgtcgta gacgacgcgg ttgaccccgc tcacctcgtt cacgatccgt gagctgatcc
     4381 tcgccagcag gtcgtagggg agccgcgccc aatcggcggt catgccgtcc tctgaggtca
     4441 ccgcgcgcac tgcgacgacg ttggcatagg tccgcccgtc gcccatcacg cccacggatc
     4501 ggaccggcgt caggatcgcg aaggactgcc acagggaccg atacaggccg gcggccttga
     4561 tctcgtcgat cacgatccag tcggcctccc gcagcgtgtc gaggcggtcg gcggtcacct
     4621 cgccgatgat gcggatcgcg aggccgggcc ccgggaaggg ctggcgcagg accatcgcct
     4681 ctggcaggcc gagctccacc cccacgttcc gcacctcgtc cttgaagagg tagcggagcg
     4741 gctcgatcag gctgaagcgc aggtcggccg gcaggccgcc gacgttgtgg tgagtcttga
     4801 tctttgccgc cgtcttcgtc tcggcggtct tcgactcgat gacatcggga tagagcgtgc
     4861 cctgggtcag gaagtcgatc tgccccagct tggccgcctc ctcctcgaag acccggatga
     4921 actcgtcgcc gatgatgcgg cgcttctctt cggggtcctc gaccccggcg agccgccgca
     4981 ggaagcgctc gcgcgcgtcg accatgacca gcttcatacc caggttctcg gcgaatgtcg
     5041 cgcgcagcag ctccgactcg cgcttgcgca tcaggccgtg atcgacgtag atgcaggtca
     5101 gttggtcccc gatcgcctgg tgcacgagcc tggccgcgac cgccgaatcg accccaccgg
     5161 agagcgcgca gatcaccttg ccgtcgccga cccgctcgcg aatcccggcc acggtcgtct
     5221 ccacgaagct cgctggcgtc caggtctggc gcgcgccggc gatgccgacg acgaagttgc
     5281 gcaggatgtc gcggccggcc ggggtgtgga ctacctcggg gtggaactgg atcccgtaca
     5341 ggccgcgcgt cggatcggcg agcccggcga aggcgctcgt ctccgtctcg gcggtggtga
     5401 tgaatccagt cggcggacgc acgatccggt cgccgtggct catccagacc ggctgggtcc
     5461 gctccaggcc acggaagagc ccatcctcgg tggtgatctg caccgatgcc ggtccgtact
     5521 cgcgccgtgt gctcggcgtc acctcgccgc cgagctggtg ggccatcagc tgcaggccgt
     5581 agcagatgcc caggaccggg atcccgccgg cccagatggc ggggtcgggg tgcggcgcgc
     5641 cgtcgtcgta gaccgaggag gggccgccgg agaggatgat cgccttcggc cggcgccgct
     5701 cgatctcggc ccacggggtg tcatggggga ggagctcact gtagacgttc agctctcgca
     5761 cgcgacgggc gatcagctgc gcgaactggc tcccgaagtc gaggatgacg atcgtatcgg
     5821 gctcggccgg aacctcctcg gcgcgctccg gcggcggtgc gtggagcgcc tgctcgaggt
     5881 acgcctcgac ctcggcgtcg tcaggggcga ggacccggtg accggcattc gccgcctcgc
     5941 cgtggaccgt gcgtccccgg gtcggcggaa atgggtcgtc cggcaacgtt ccctccctgc
     6001 tcgcgtgccg ctattccctg gcggccagct gccaggactt gccctcggtc gcgatcgaag
     6061 gggcgatcac catctcgacc cgatgcatct cgcggatggt gcgcgccccc agcgaggcca
     6121 tcgactggcg gagcgcaccc accaggttct gcgtcccatg cgtggtgtgg gcagggccga
     6181 agaggatctg ctccagcggc agccgctccc cgatcttgat tcgcgtcccg cgcggcaggg
     6241 tcgg
//
LOCUS       DATCQR010000214         5668 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_74404, whole genome shotgun sequence.
ACCESSION   DATCQR010000214 DATCQR010000000
VERSION     DATCQR010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5668
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74404"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="VIN32_07150"
     CDS             complement(<1..242)
                     /locus_tag="VIN32_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEY8922387.1"
                     /translation="MGAPLRILVAPDSFGGALDSVAVARAIGAGWLRARPDDEVIQAP
                     MADGGEGTLAAVATALGERAVRRSVAAHDALGRPISV"
     gene            complement(242..2191)
                     /locus_tag="VIN32_07155"
     CDS             complement(242..2191)
                     /locus_tag="VIN32_07155"
                     /EC_number="2.4.1.266"
                     /inference="COORDINATES: protein motif:HMM:NF010496.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyl-3-phosphoglycerate synthase"
                     /protein_id="HEY8922388.1"
                     /translation="MARRRQPYRILLPLANPRTARDLVRIGAGIGQGRLTEITALGIV
                     EVPEGVSLSEGATHARTARRLLQRVLDFGDEEEVEIRTMVRIGRRAADGVIEAVGEES
                     ADLVIFGWGGPPSAAAAKRPTTTRGAQTEPAVFSPTIDAVVRESPSDIAVVKQRGLDK
                     VSSILVPVRGGPHAELAMRLARDLGKRFGAQVVVMHVVPKGIGERALAREQVALDRFV
                     AEHRAGRRVIGILREAASVRAAILREASNHQLVVMGASANPSTMTPDGRFLFGVVAEA
                     VASRAKPTVIVVKTKQELAVSLFDDLRAAEGTLAAADAFVEDGRALPTVVDRWFAENT
                     FHASEFRDVAKLVELKEKQGVTISLGLPALNEEKTIGLVIKRIKGALMDRVPLLDQLV
                     LIDSDSTDRTREIAAELGVPVVRHSQILPEMGSFVGKGEALWKSLHVLDGDIVAWIDT
                     DIRNIQPRFVHGLLGPLLREPRIQYVKGFYRRPIRQGLTLFAEGGGRVTELMARPLIN
                     LFFPELSGFIQPLSGEYAGRRALLDSVPFFTGYGVEIGLLIDIAETAGLSAIGQVDLE
                     RRIHRNQPLSNLSQMSFTILQAAITKLEERHRIELLTEVGRGMKVIGQEKDRFNLEVR
                     EVADSVRPPMRSVPAYQERRRSLKR"
     gene            complement(2191..3414)
                     /locus_tag="VIN32_07160"
     CDS             complement(2191..3414)
                     /locus_tag="VIN32_07160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HEY8922389.1"
                     /translation="MVGIPSFGNAETIGFVVRAATAGMVQYFPDLKPILVNSDGGSPD
                     HTPRVAITAESPEYIEKIILVSPRHKLQRITFTYHGLSGKGTAVRALFEVARELGVKA
                     MVMVDSDLRSIAPEWIELLAGPILKGGYDFVAPLYARYKYDGTITNAIAYPLTRALYG
                     ARIRQPIGGEFAVSGDLVQRYLELDSFDELTARFGIDVWMTHSAINEGFAVCQTRLGA
                     KIHDAKDPAGDLGPMFRQVVGTLFALAGRYQDRWLKVRGSHSIPEYGFERVIAPEVVE
                     VNHAKLVDNFETARLSQVDAWAQILSPDQLDRVMSVEIPSEPGAFHFPADLWIRCLYD
                     ALVAYNREGTDRERLLAALTPLYFGRTAGFIAETIEMTTDQAERVIEAVAREFEELKP
                     YLVTRWREMRAQAAG"
     gene            complement(3560..4714)
                     /locus_tag="VIN32_07165"
     CDS             complement(3560..4714)
                     /locus_tag="VIN32_07165"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY8922390.1"
                     /translation="MDAVLAAQRSHRFGEIELPVTIARAIEKMGFTAPTEVQARAIGP
                     LRAGRDLIGQAQTGTGKTAAFGIPIVEKVDPSGRFVQALVLTPTRELCVQVTEEIRRI
                     GVFRNVAAVAIYGGSSMDRQVTALKRGVPVVVGTPGRVLDLIRRGELRLDRVHTVILD
                     EADRMLDMGFIVDVETILSRCPRNRQTALFSATMPYAILRICDRFMDKQAEHIIVRPE
                     MRTVETVRQLVYFVAEQDKVAALTELTDQFGFDRLLVFRHTQIGVDRLTAILARKGRN
                     VAALHGGLSQRERDKTLADFRSGKIQFLVATNVASRGLDITDLPFVLNYDIPEDADTY
                     VHRVGRTARAGREGTAITLVGEWDLDAWEQIRKEVGVEVEEGELKLYHRS"
     gene            complement(4888..>5668)
                     /gene="galE"
                     /locus_tag="VIN32_07170"
     CDS             complement(4888..>5668)
                     /gene="galE"
                     /locus_tag="VIN32_07170"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015253901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEY8922391.1"
                     /translation="LEAMRLTGVNRLVFSSTAAVYGEPRRVPIAESDRTEPINPYGAS
                     KLAFEGAMRWFAASHDFQAISLRYFNVAGATERNGEDHEPETHLMPLMLRVAAGEATH
                     VQIFGQDYPTPDGTCIRDFIHVRDLAAAHLRALEVTGEGDPSFEVYNLGSAAGFSVRE
                     VVEAARKVTGRAIPARVVKRRTGDPPVLVASSRRARLALGWHPEHSKLEQMLADAWAW
                     KLTHPNGYARGPRTDGAALDRQPSDVRGDRQISRPGAPASV"
BASE COUNT          872 a         1930 c         1989 g          877 t
ORIGIN      
        1 acagagatcg gcctgcccaa ggcgtcatga gccgcaaccg accgccgcac agcgcgctcg
       61 ccaagcgccg tggcgaccgc cgcgagcgtg ccttcgccac cgtcggccat gggggcctgg
      121 atcacctcgt cgtccggtcg agcccgcagc cagcccgccc cgatggcgcg ggccaccgcc
      181 acggagtcga gggcaccgcc gaacgaatcg ggagccacca ggatgcgcag gggggcgccc
      241 atcaccgctt cagggagcga cgacgttcct ggtaggcggg gacgctgcgc attggcggac
      301 gaaccgagtc ggcgacctcg cggacctcca ggttgaatcg gtccttctcc tgcccgatca
      361 ccttcatgcc gcgcccgacc tcggtcagca gctcgatccg gtgccgctcc tcgagcttgg
      421 tgatggcggc ctgaaggatc gtgaagctca tctgcgagag gttggagagc ggctggttgc
      481 gatggatccg ccgctcgagg tcgacctggc cgatcgccga cagcccggcc gtctcggcga
      541 tgtcgatcag cagcccgatc tcgaccccgt agccggtgaa gaacgggacc gaatcgagga
      601 gggcgcgacg accggcgtac tcacccgaga gcggctggat gaatccggag agctcgggga
      661 agaagaggtt gatcaagggc cgtgccatca gctcggtcac gcgtccgcca ccctcggcga
      721 agagggtcag gccctggcgg atcgggcgcc ggtagaagcc cttcacgtac tggatgcgcg
      781 gctcgcgcag gagcgggccg agcagcccgt gcacgaagcg tggctggatg ttgcggatgt
      841 cggtgtcgat ccaggccacg atgtcgccgt cgagcacgtg gaggctcttc cacagtgcct
      901 cgcccttccc gacgaacgat cccatctccg gcaggatctg tgaatgccgc acgacgggga
      961 caccgagctc agcggcgatc tcgcgggtgc ggtcggtgct gtcgctgtcg atcaggacca
     1021 gctggtccag cagcggcacg cgatccatca gcgcgccctt gatgcgcttg atgacgaggc
     1081 cgatcgtctt ctcctcgttg agggccggaa gcccgaggct gatcgtgacg ccctgcttct
     1141 ccttgagctc gacgagcttg gccacgtcgc ggaactcgga ggcgtggaac gtgttctcgg
     1201 cgaaccagcg gtcgacgacg gtgggcagcg cccggccgtc ctcgacgaac gcatcggcgg
     1261 cggcaagcgt cccctcggcc gcacgcaggt cgtcgaagag cgagaccgcc agctcctgct
     1321 tggtcttgac cacgatcacc gtcggcttgg cgcgtgatgc gaccgcctcg gccaccaccc
     1381 cgaacaggaa ccgtccgtcc ggggtcatgg tcgacggatt ggccgatgcg cccatcacca
     1441 ccagctggtg attgctcgcc tcgcgaagga tcgctgcgcg caccgatgct gcctcccgca
     1501 ggatgccgat caccctgcgc ccggcgcggt gctccgccac gaaccggtcg agcgccacct
     1561 gctcgcgggc cagggctcgc tccccgatcc ccttgggcac cacgtgcatg accactacct
     1621 gggctccgaa gcgcttgccc aggtcgcgcg ccagtcgcat cgccagctcg gcatgcggac
     1681 cgccacgcac cgggaccagg atcgagctga ctttgtccag gccgcgctgc ttgacgaccg
     1741 cgatgtccga cggagactca cgcaccacgg catcgatcgt cggcgagaag acggccggct
     1801 cggtttgagc gccccgagtg gtggtcggtc gcttggcggc ggcagcgctc gggggtccgc
     1861 cccacccgaa gatcacgagg tccgcgctct cctcaccgac cgcctcgatc acaccgtcgg
     1921 ccgcccggcg cccgatcctg accatggtcc ggatctcgac ctcctcttcg tcgccgaagt
     1981 cgaggacgcg ctgcagcagc cggcgggccg tgcgcgcgtg ggtcgccccc tcggacagcg
     2041 agactccctc cggcacctcc acgatcccga gcgcagtgat ctcggtcagc cggccctgcc
     2101 cgatccccgc gccgatgcga accaggtcac gggcagtgcg cgggttggcg agcggcagca
     2161 ggatccggta cggctggcgc cggcgggcca tcagcctgcc gcctgggcgc gcatctcgcg
     2221 ccagcgggtg acgagatacg gcttcagctc ctcgaactcg cgcgcgaccg cctcgatcac
     2281 ccgctcggcc tggtcggtcg tcatctcgat cgtctcggcg atgaacccgg cggtccgccc
     2341 gaagtagagc ggggtgagcg cggccagcag ccgctcgcgg tcagtcccct cccggttgta
     2401 ggcgaccagg gcgtcgtaca ggcagcggat ccacaggtcg gccgggaagt ggaaggcgcc
     2461 cggctccgaa gggatctcaa ccgacatgac gcggtcgagc tggtctggcg agaggatctg
     2521 cgcccaggca tcgacctgcg agaggcgcgc cgtctcgaag ttgtcgacca gcttcgcgtg
     2581 gttgacctcg accacctccg gggcgatcac ccgctcgaac ccgtactcgg ggatggaatg
     2641 tgagccgcgg accttgagcc atcggtcctg gtagcggccg gcaagcgcga acagggtccc
     2701 gaccacctgg cggaacatcg gcccaaggtc gccggcgggg tccttggcgt cgtggatctt
     2761 ggcgcccagc cgcgtctggc agacggcgaa tccctcgttg atggccgagt gcgtcatcca
     2821 cacgtcgatc ccgaagcgcg ccgtcaactc gtcgaagctg tcgagctcca ggtagcgctg
     2881 caccaggtcg ccgctcacgg cgaactcgcc gccgatcggc tggcggatgc gcgccccgta
     2941 cagcgcccgg gtgagcgggt aggcgatcgc gttggtgatc gtgccgtcgt acttgtagcg
     3001 agcgtagagc ggcgccacga agtcgtagcc gcccttgagg atcggtccgg ccagcagctc
     3061 gatccattcc ggcgcgatgc tgcgcaggtc cgagtcgacc atcaccatcg ccttcacccc
     3121 aagctcgcgc gcgacctcga agagggcccg caccgccgtc cccttgccac tcaaaccgtg
     3181 gtaggtgaag gtgatccgct gcagcttgtg gcgcgggctg accaggatga tcttctcgat
     3241 gtactccggg ctttcggccg tgattgcgac ccgtggcgtg tggtcgggag agcctccgtc
     3301 ggagttgacc aggatcggct tgaggtcggg gaagtactgc accatcccgg ccgtggcggc
     3361 acgcaccacg aagccgatcg tctcggcgtt gccgaagctg gggatgccga ccatcaggtc
     3421 cgcctggccg atgcggcgga cctcggcgag ccctctcgcg tcgagcgcag aagggattgg
     3481 cgaagtggaa cggttggtct tcagcgctga gacctcgtca tgcgcagcaa gggtagcgcg
     3541 cccggcgagg ccggtcaggt caggatcggt ggtagagctt cagctcgcct tcttcgacct
     3601 cgaccccgac ctccttgcgg atctgctccc aggcgtcgag gtcccactcg ccgaccaggg
     3661 tgatggccgt cccctcgcgt ccagcgcgcg cggtgcggcc gacgcggtgc acgtaggtgt
     3721 cggcgtcctc cgggatgtcg tagttgagga cgaacggcag gtcggtgatg tccagcccgc
     3781 gggacgcaac gttggtcgcg accaggaact ggatcttgcc cgagcggaag tcggcgagcg
     3841 tcttgtcgcg ctcgcgctgc gagaggccgc cgtgcagcgc ggccacgttg cgccccttgc
     3901 gagcgaggat ggcggtcagg cgatcgaccc cgatctgggt gtgtcggaag accaggagcc
     3961 gatcgaaccc gaactggtcg gtcagctcgg tgagcgccgc aaccttgtcc tgctcggcca
     4021 cgaagtagac cagctggcgc accgtctcca cggtccgcat ctccggccgc acgatgatgt
     4081 gctcggcctg cttgtccatg aaccgatcgc agatgcgcag gatcgcgtac ggcatcgtgg
     4141 ccgagaagag cgccgtctgg cgattgcggg gacagcgcga caggatcgtc tcgacgtcaa
     4201 cgatgaaccc catgtcgagc atccggtcgg cctcgtcgag gattacggtg tgcacccggt
     4261 ccaggcgcag ctctccacgg cggatcaggt ccaggactcg gcccggcgtc ccgaccacga
     4321 cggggacgcc ccgcttcagg gcggtcacct ggcggtccat gctggagccg ccgtagatgg
     4381 cgacggcggc aacgttgcgg aagacgccga tgcgccggat ctcctccgtc acctgcacgc
     4441 atagctcgcg ggtcggggtg aggaccaggg cctgcacgaa gcggcccgat ggatccacct
     4501 tctcgacgat ggggatgccg aacgcggcgg tcttgccggt tccggtctgc gcctggccga
     4561 tcaggtccct gccggcgcgg agcgggccga tggcgcgggc ctggacctcg gtcggggcgg
     4621 tgaagcccat cttctctatg gccctggcaa tcgtgaccgg cagctcgatc tcgccgaagc
     4681 gatgggagcg ctgcgccgcc agtacggcgt ccatcagcgg ggatgattcg aaatcgtcag
     4741 gcgagggatt gccgttgggt gctggcgatg gcggcggcgc cgctcggcgg ggcccacggg
     4801 cgcgtcgcgg aggggcggga gaggtggttt caggcaaacg gggctccggg ggagggtaac
     4861 ttgatctcga tcgtaccacg cggcgcccta gaccgaggca ggggcacctg gccgcgagat
     4921 ctggcggtcg ccgcggacat ccgacggctg gcggtcgagc gccgccccat cagttcgcgg
     4981 acccctggcg tagccgttcg gatgcgtgag cttccacgcc catgcatcgg ccagcatctg
     5041 ctcgagcttg gaatgctcgg ggtgccagcc aagggcgagc cgcgctcggc gcgacgaggc
     5101 caccagcacg ggcgggtcgc cggtgcgacg cttcacgacg cgggccggga ttgcgcggcc
     5161 ggtcaccttg cgcgccgcct cgaccacctc gcggacgctg aagcccgcgg cggagccgag
     5221 gttgtagacc tcgaacgacg ggtcaccctc accggtcacc tccagcgcgc gcaggtgcgc
     5281 ggcggccagg tcacggacgt ggatgaagtc gcgaatgcag gtcccgtcgg gggtcgggta
     5341 gtcctgtccg aagatctgga cgtgcgtcgc ctcgcccgca gccacccgca gcatgagggg
     5401 catgaggtgc gtctcgggct cgtgatcctc gccattgcgc tcggtggcgc cagcgacgtt
     5461 gaagtaccgg aggctgatcg cctggaagtc atggctggcg gcgaaccagc gcatcgcgcc
     5521 ctcgaaggcg agcttcgagg ccccgtatgg gttgatcggc tcggtccggt ccgactcggc
     5581 gatgggaacc ctgcgcggct cgccgtagac cgctgcggtc gaggagaaga ccagccggtt
     5641 gacgccggtg agacgcatgg cctccaac
//
LOCUS       DATCQR010000215         3552 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_89828, whole genome shotgun sequence.
ACCESSION   DATCQR010000215 DATCQR010000000
VERSION     DATCQR010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3552
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89828"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..337)
                     /locus_tag="VIN32_07175"
     CDS             complement(<1..337)
                     /locus_tag="VIN32_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018132816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEY8922392.1"
                     /translation="MATTIKMPQLGESVTEGTIERWLVKEGDKVEQYDPLFEVVTDKV
                     NAEVPAEVAGTVTKIIVGDGQTVKVGTPLAEIETDGATAEAPAAAPPAAAATAPPEVE
                     PEPVPEPTPT"
     gene            complement(620..1606)
                     /locus_tag="VIN32_07180"
     CDS             complement(620..1606)
                     /locus_tag="VIN32_07180"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017248037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEY8922393.1"
                     /translation="MAELNILEAIRTAMDEELERDPNVMVLGEDVGKKGGVFGATEGL
                     WAKYGGKRVLDTPLSEAMIAGAAAGAAIYGLRPIAEMQFADFSYPAFNQIVSEISRMR
                     YRSNNAFGLPMVIRMPYGGGVHGALYHSQSNEAYFTSTVGLKVVAPGTPADAKGLLKA
                     SVRDPDPVMFFEHKKTYRLFKEEVPDGDHVVPIGVAEVARPGKHLSIFCYGYMRFCAL
                     EAAETLAREDGVEAEVVDLRTLRPLDTEAVLASVAKTGKALVVHEANRFGGFGAEVAA
                     IIAEQAFEHLDGPVTRLAGPEVPGVPFHHSLEDWFMLNPAKIVEAARGLAAY"
     gene            complement(1606..2622)
                     /locus_tag="VIN32_07185"
     CDS             complement(1606..2622)
                     /locus_tag="VIN32_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEY8922394.1"
                     /translation="MANTKVAELGHQALGLTDADVLAMYRQMLLARAVDERMWLMQRA
                     GKIAFIISGQGHEAAQIGTTWPMRPNQDWMAPYYRSIASAITFGMSPEDIVTAHLAKG
                     DDVSSGGRQMPGHYGAARYHIVSLSSPVGTQVLHAVGIAMGAWVKGEDVVAITSFGEG
                     TSNQGEIHEAMNFAGVHKLPVIFVCENNGYAISVPLDQQVGGLSVAARAQGYGFPGVT
                     VDGTDPLACYAAAKEAHERATRGEGPTLIEAVVHRLTSHSSDDDQRRYRDAAEVEELK
                     QHDPIPLFATRLVSAGLLTAELEEQYRVEVKAEINEASKRAEARPEPDIATAERWVYA
                     EEGG"
     gene            complement(2636..>3552)
                     /locus_tag="VIN32_07190"
     CDS             complement(2636..>3552)
                     /locus_tag="VIN32_07190"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY8922395.1"
                     /translation="ALLRETVPARVAIVGAGAIGVEWASLYSDLGAEVHLIEYLERIV
                     PLEDEDVSKELTRIFKKRNVKIYAASSIDPKSIERTKRGVHFAVEARDGKTRVEMETD
                     VILVAVGRRPVTEGIGLEDIPGVEMERGFVKVNGFMQTGVPHLYAIGDIVPGYALAHV
                     ASHEAVVAVEKIAGHDPDPVRMELMPRVTFCRPEIASVGMTEAQATDAGHTVKVGSFP
                     FRALGKATIVGEVDGFAKLVADADTGLLLGAHLIGAHAGDLLAEPTLALLLEGTAGEI
                     AMSVHAHPTLTEVLVEAALAVDGAAIHV"
BASE COUNT          495 a         1248 c         1211 g          598 t
ORIGIN      
        1 gcgtcggggt gggctccggg acgggctccg gctcaacttc cgggggtgcg gttgcggcgg
       61 cggccggggg agcagcggct ggggcctcgg ccgtggcgcc atcggtctcg atctcggcca
      121 gcggcgtacc aaccttcacc gtctgaccgt cgcccacgat gatcttggtg acggtgcctg
      181 cgacctcggc ggggacctcg gcgttgacct tgtcggtgac cacctcgaag agcgggtcgt
      241 actgctcgac cttgtcgccc tccttgacca gccagcgctc gatcgtgccc tcggtgactg
      301 actcgccgag ctgcggcatc ttgatggtgg tcgccatggt ctctccgctc tcctaggctg
      361 gcgcgcggcc ggccggcgcg cttgcagttc tcatgttgcg ctcacgctgg cgcatcgcca
      421 aatcgcagtc tggttgacct tagccgctct gaaccaggca tgcgcggtgt ccttcaagct
      481 tcgtcgagcg tgaaaagggc cgatgcgtac cgccccgcgg gctaaaacgc gcctaatggc
      541 aatcgggttg tgatctggcg acatgacgtc tcccgaggac cgccgcgacc gccgcgaccg
      601 ccgtgaccgc cgcatcggct cagtaggcgg caagcccacg ggccgcctcg acgatcttgg
      661 cggggttgag catgaaccag tcctcgaggc tgtggtggaa cgggacgccg ggcacctccg
      721 gcccggcgag gcgcgtcacc gggccgtcga ggtgctcaaa cgcctgctcg gcgatgatgg
      781 ccgccacctc cgccccgaag ccaccgaagc ggttcgcctc gtggacgacc agggcctttc
      841 cggtcttggc caccgatgcc agcaccgcct ctgtgtccag cggacgcagc gtgcgcaggt
      901 ccaccacctc ggcctcgacg ccgtcctcgc gggcgagcgt ctccgccgcc tccagcgcgc
      961 agaagcgcat gtagccgtaa cagaagatcg agaggtgctt gccgggtcgg gccacctcgg
     1021 ccacgccgat cggcaccacg tgatcaccat cggggacctc ctccttgaag aggcggtacg
     1081 tcttcttgtg ctcgaagaac atcaccggat cggggtcgcg gaccgaggcc ttgagcaggc
     1141 ccttggcatc ggccggggtg cccggggcca ccaccttgag gccgacggtg gaggtgaagt
     1201 aggcctcgtt gctctgcgag tgatagagcg cgccgtgcac gccgccgccg tacggcatgc
     1261 ggatcaccat cggcaggcca aaggcgttgt tggaccgata ccgcatccgg ctgatctcgg
     1321 acacgatctg attgaaggcg gggtaggaga agtcggcgaa ctgcatctcg gcgatggggc
     1381 gcaggccata gatcgcagcc cccgcggccg cgcccgcgat catcgcctcg ctgaggggcg
     1441 tgtcgagcac ccgcttgcct ccgtacttgg cccacagccc ctcggtggcg ccgaagacgc
     1501 cgcccttctt gccgacgtcc tcgcccagga ccatcacgtt cgggtcgcgc tccagctcct
     1561 cgtccatggc ggtgcggatc gcctcgagga tgttcagctc ggccatcagc cgccctcctc
     1621 ggcgtagacc cagcgctcgg ccgtggcgat gtccggctcg gggcgcgcct cggcgcgctt
     1681 ggaggcctcg ttgatctccg ccttgacctc cacgcggtac tgctcctcga gctcggccgt
     1741 caacaggcca gcgcttacca gccgggtggc gaagagcggg atcgggtcgt gctgcttgag
     1801 ctcctcgacc tcggctgcat cgcggtagcg gcgctgatcg tcgtccgacg agtggctggt
     1861 gaggcggtgc acgacagcct cgatcagggt cggcccctcg ccccgtgtcg cccgctcgtg
     1921 cgcctctttg gcggcggcgt agcaggccag cgggtcggtg ccatccaccg tgacgccggg
     1981 gaagccgtag ccctgtgcgc gggccgcgac cgacaggccg ccgacctgct ggtcgagcgg
     2041 cacgctgatg gcgtagccgt tgttctcgca cacgaagatg accggcagct tgtgcacgcc
     2101 ggcgaagttc atcgcctcgt ggatctcgcc ctggttgctc gtcccctcgc cgaacgaggt
     2161 gatcgccacc acgtcctcgc ccttgaccca ggcgcccatg gcgatcccca ccgcgtgcag
     2221 cacctgggtg ccgaccgggg agctgagcga gacgatgtgg tagcgcgcgg cgccgtagtg
     2281 gcccggcatc tgacgcccgc cggacgacac gtcgtctccc ttggccaggt gcgcggtgac
     2341 gatgtcctcg gggctcatgc cgaaggtgat cgcggacgcg atcgagcggt agtagggagc
     2401 catccagtcc tggttcgggc gcatcggcca ggtggtgccg atctgtgccg cctcgtggcc
     2461 ctggccggag atgatgaagg cgatcttgcc ggcgcgctgc atcagccaca tccgctcgtc
     2521 gaccgcgcgg gcgagcagca tctggcggta catggccaga acgtcggcgt cggtcaggcc
     2581 gagcgcctgg tgtccaagct ccgcgacctt ggtattcgcc atcacgccca ttgtctcaga
     2641 cgtggatcgc cgcgccatcc acggcgagcg cggcctcgac cagcacctcg gtgagggtcg
     2701 ggtgcgcgtg gaccgacatc gcgatctccc cggcggtccc ctccagcagc agggccagcg
     2761 tcggctcggc gaggaggtcg cccgcgtggg caccgatcag gtgcgcgccg agcaggaggc
     2821 cggtgtcggc atcggcgacg agcttggcga agccgtcgac ctcgcccacg atggtggcct
     2881 tgcccagcgc gcggaagggg aatgagccga ccttgacggt gtggcccgca tcggtcgcct
     2941 gggcttcggt cattccgacc gaggcgatct ccggccggca gaaggtgacc cgcggcatga
     3001 gctccatgcg caccgggtcg gggtcgtggc ccgcgatctt ctcgaccgcg acgaccgcct
     3061 cgtggctggc cacgtgcgcc agggcatagc ccggcacgat gtcgccgatc gcgtacaggt
     3121 gcgggacccc ggtctgcatg aagccgttga ccttgacgaa gccgcgctcc atctcgacgc
     3181 cgggaatgtc ctccaggccg atcccctccg tcacggggcg cctgccgacc gccaccagga
     3241 tgacgtccgt ctccatctcg acccgcgtct tgccgtcccg cgcctcgacc gcgaaatgca
     3301 ccccgcgctt ggtgcgctcg atgctcttgg ggtcgatgct cgacgctgcg tagatcttga
     3361 cgttccgctt cttgaagatg cgggtcagct ccttgctgac gtcctcgtcc tcgagcggca
     3421 cgatgcgctc gaggtattcg atcaggtgca cctccgcacc caggtcgctg tacaggctgg
     3481 cccattcgac gccgatggcg ccggcgccca cgatggccac gcgcgccggg accgtctcgc
     3541 gcagcagcgc ct
//
LOCUS       DATCQR010000216         2413 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_27505, whole genome shotgun sequence.
ACCESSION   DATCQR010000216 DATCQR010000000
VERSION     DATCQR010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2413
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27505"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(132..344)
                     /locus_tag="VIN32_07195"
     CDS             complement(132..344)
                     /locus_tag="VIN32_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8922396.1"
                     /translation="MTDLAPNVLYYGDNLDILRRYLPDAAVDLVSLDPPFNSNRDYNV
                     IFRDESGNATDAQLLAFEDTARRTGR"
     gene            complement(341..733)
                     /locus_tag="VIN32_07200"
     CDS             complement(341..733)
                     /locus_tag="VIN32_07200"
                     /inference="COORDINATES: protein motif:HMM:NF024297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY8922397.1"
                     /translation="MTGNEGAISPEDRQRIVASATDYIESWIAGDADRMAACMHPELI
                     KRSVEPDPQTGGTIVRTLTRARMVALTAAGEGAEDAGPYQVSVLDAYGDIATVSVLSA
                     AYMDYLQVARFGDAWLILNVLWQPRIGR"
     gene            complement(735..1088)
                     /locus_tag="VIN32_07205"
     CDS             complement(735..1088)
                     /locus_tag="VIN32_07205"
                     /inference="COORDINATES: protein motif:HMM:NF017690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY8922398.1"
                     /translation="MTEPEPGVFMTSLSTAEWEPDPDVGGLMHILCDVPGVWAGFTRF
                     DTTPEPVPWTPLQRETFMILEGAVRIEIANGATLEMHSGDIASLPAGTETTWHITAPF
                     REFWVLSGTQVEEEA"
     gene            complement(1085..1264)
                     /locus_tag="VIN32_07210"
     CDS             complement(1085..1264)
                     /locus_tag="VIN32_07210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8922399.1"
                     /translation="MTNLATNVLCSGDNLDILRRYLPDAAVDLVYPDPPDATDAQLLA
                     FEDTWHCGPSAEATQ"
     gene            1457..1708
                     /locus_tag="VIN32_07215"
     CDS             1457..1708
                     /locus_tag="VIN32_07215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEY8922400.1"
                     /translation="MATVGERYQVVIERDARRELGISPGDRAVETVENGRLVITFLPA
                     RHRRSLLGSLAGTGRVVDASVERDAMADTLAREDAERGR"
     gene            1705..2109
                     /locus_tag="VIN32_07220"
     CDS             1705..2109
                     /locus_tag="VIN32_07220"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEY8922401.1"
                     /translation="MIALDSSALLRYLLADDRAMARRVERALAGREQVGISTPVLVEV
                     VHVLRGDPYRRENPAVADAVIELLGHQNLVLTDLDPDLAAAAITAVRHLSPRHIADAL
                     ISAAARQAGARQLLTADRTFASQLVPLVQLGD"
     gene            complement(2076..2237)
                     /locus_tag="VIN32_07225"
     CDS             complement(2076..2237)
                     /locus_tag="VIN32_07225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8922402.1"
                     /translation="MSCEFTTSWIVRDNLDILRRYLPAAAVDLVYLDPPFNSDRQKAQ
                     SPSWTNGTS"
     gene            2221..>2413
                     /locus_tag="VIN32_07230"
     CDS             2221..>2413
                     /locus_tag="VIN32_07230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8922403.1"
                     /translation="MNSQDINLDEIPEFDFSNAVRGTYAEWAKRAKGHFYEVTDEEDA
                     KRFAAERRPKGLLKKLSRPK"
BASE COUNT          388 a          748 c          870 g          407 t
ORIGIN      
        1 gcctaaaggt atccctcagg gatgggccaa cgaccgtcgt tcgtctctcc gcccgctgcc
       61 ttcaccgtca tcgcgcagtt gcccaggtcg atggagcggt cctcgccggg tagcaggatg
      121 agcggtcgct ctcacctccc ggttctcctg gctgtgtcct cgaaggccag cagctgggca
      181 tcggtggcat tgcccgactc gtcgcggaag atgacgttgt agtcgcggtt ggagttgaag
      241 ggcgggtcga gggagaccag gtcgacggcg gcgtcgggca ggtagcgacg caggatgtcg
      301 aggttgtcgc cgtagtagag gacgttgggg gcgaggtcgg tcatcggcct atgcgtggct
      361 gccagagcac gttgaggatg agccaggcgt cgccgaaccg ggcgacctgg aggtagtcca
      421 tgtaggccgc cgacaggacg ctgacggtgg cgatgtcacc gtacgcatcc aggaccgaga
      481 cctgatacgg gcctgcatcc tccgcccctt cgcccgcggc ggtcagcgcg accatcctgg
      541 cgcgggtcag ggttcgcacg atcgtgccgc cggtctgcgg atccggttca accgaccgct
      601 tgatgagctc ggggtgcatg caggcggcca tccgatcggc gtcgccagcg atccacgatt
      661 cgatgtaatc cgtggcggac gcgacaatcc tttggcggtc ttcgggactg attgcccctt
      721 cgtttccggt caactcaagc ctcctcttcg acctgcgtgc cgctgagcac ccagaattcg
      781 cgaaacggtg cggtgatgtg ccacgtggtc tccgtgcccg ctggcaggga ggcgatgtcg
      841 ccactgtgca tctccagcgt cgcgccattg gcgatctcga tcctgaccgc gccctccagg
      901 atcatgaagg tctcgcgttg gagtggagtc cagggcaccg gctcgggcgt ggtgtcgaaa
      961 cgtgtgaagc cggcccagac ccccggcacg tcgcaaagga tgtgcatcag gccgccgaca
     1021 tcggggtcgg gctcccattc ggccgtggag aggctcgtca tgaacacccc gggctccggt
     1081 tccgtcactg ggtcgcctcg gccgagggac cgcagtgcca ggtgtcctcg aaggccagca
     1141 gctgggcatc ggtggcatca ggcgggtcgg ggtagaccag gtcgacggcg gcatcgggca
     1201 ggtagcgacg caggatgtcg aggttgtcgc cggagcagag gacgttggtg gcgaggttgg
     1261 tcatcggtcc cccgcgaggc gtggctcagg tgggcgccgg agcctgtgga gagggtagca
     1321 gcgagggcaa gcgatcggcg gtcggtaagc gggcggtaag ccgtcatcgc gaacattgga
     1381 tggggtgagg ggcgagcatc gagtatgcct ttctgatgct tgacatacat atgcggacgt
     1441 tggcatactc ggtgccatgg caactgtggg agagcgatac caggtggtga tcgagcggga
     1501 tgcccggcgc gagttgggca tcagcccggg ggatcgcgcg gtggagacgg tcgagaacgg
     1561 ccggctggtc atcaccttct tgccggcccg ccaccggcga tccctgctgg gaagcctcgc
     1621 cgggactggg cgcgtggtcg acgcatcggt cgagcgtgac gccatggcgg acaccctggc
     1681 gcgggaggac gcggagcgcg ggcggtgatc gccctcgaca gcagcgcgct ccttcgctat
     1741 ctgctggccg acgaccgggc catggcgcgg cgcgtggagc gtgcgcttgc tggccgcgag
     1801 caggtcggaa tctccacgcc ggtactcgtc gaagtggtgc atgtgctccg cggcgacccc
     1861 taccggcgcg agaacccggc cgtggccgat gccgtcatcg agctcctcgg gcaccagaac
     1921 ctggtcctca ccgacctgga cccggacctg gccgccgccg ccatcaccgc cgtccggcac
     1981 ctctcgccgc gccacatcgc cgacgcgctg atcagcgctg ccgcccggca ggccggtgct
     2041 cggcagctcc tcacggctga ccgcaccttc gccagtcagc tcgtgccgtt ggtccagctg
     2101 ggggattgag ccttctggcg atcggagttg aagggcgggt cgaggtagac caggtcgacg
     2161 gcggcagcgg gcaggtagcg acgcaggatg tcgaggttgt cgcggacgat ccatgacgtc
     2221 gtgaattcac aggacatcaa cctcgacgag atcccggagt tcgacttctc gaacgcggtc
     2281 cgaggaacgt acgccgagtg ggccaagcgc gccaagggtc acttctacga ggtgaccgat
     2341 gaagaggacg ccaagcgctt cgcggccgag cgccgaccga aggggcttct caagaaactg
     2401 agtcgcccga aac
//
LOCUS       DATCQR010000217         8367 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_43277, whole genome shotgun sequence.
ACCESSION   DATCQR010000217 DATCQR010000000
VERSION     DATCQR010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 8367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8367
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43277"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..394)
                     /locus_tag="VIN32_07235"
     CDS             complement(<1..394)
                     /locus_tag="VIN32_07235"
                     /inference="COORDINATES: protein motif:HMM:NF012799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEY8922404.1"
                     /translation="MAGRLLVVGTPIGNLGDLTPRAAEALRTADLVVAEDTRMARRLL
                     AHLGAKRPTVSYNDHSPETRKSELLDRLAGGATLAFTTDAGMPGVSDPGARLVEAARA
                     AGASVEVLPGPSAVTAAIAAAGVESLGFV"
     gene            complement(402..1256)
                     /locus_tag="VIN32_07240"
     CDS             complement(402..1256)
                     /locus_tag="VIN32_07240"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008337609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HEY8922405.1"
                     /translation="MTAPLPEIDAPSALEVITAFIRSQAEQTGFRRLVVGLSGGVDSA
                     TVAFLAARAIGADNLLAVRMPYRSSSPDSETDAMRVVAALGCRTERVDITPMVEPMLA
                     LISGDDDAALRVRRGNVMARQRMIVLYDRSAAFDALVVGTSNKTEALLGYGTLHGDMA
                     SALAPIGDLYKSQLRAVAAALGVPAEILAKPPSADLWPDQTDEAELGASYDDLDRILF
                     ALVDRRWRVERCVAAGMDARLVAWVAERVARNEFKRQTPPVAKVSLRTPGIDHLYPRR
                     RPGSRRGS"
     gene            complement(1253..2140)
                     /locus_tag="VIN32_07245"
     CDS             complement(1253..2140)
                     /locus_tag="VIN32_07245"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEY8922406.1"
                     /translation="MEYRIGLAQYAPRLGDVGANLELAAGWSRRAMTEGAELVAFPEL
                     ALTGYMLSDLVPDVAMRADDPRLAALSREAPGVVLAIGFVEETDAHRFCNSAALLRDG
                     ELIGLHRKVYLPTYGMFDEGRFTRAGDRIRTHAVGEPLGSIGLSICEDFWHASLPMLQ
                     ALDGASLLVNLAAGPARAPGSSAGLAAIAGWHKMQDTYALLGTVAVAFCNRVGNEEGL
                     TFWGGSRLLGADGSTIAQGPLYEEALVVGSIDSDDLRTQRYGLPLLSDERLELVRREL
                     DRIIAGRAGLPSPPDQDDA"
     gene            complement(2149..3348)
                     /locus_tag="VIN32_07250"
     CDS             complement(2149..3348)
                     /locus_tag="VIN32_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019058538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HEY8922407.1"
                     /translation="MTTRTSDVLDVTDLRTYFPVKSRGLISRTVGAVKAVDGVSLSLD
                     AGETLGLVGESGSGKTTAARSILMLVRPTGGSVKIDGEEITDLSARNLMPIRRKAQMI
                     FQDPYNALNPRHTVGAIISAPFKVQKVTPPNGVKQGVLELMERVGLNPEHYNRYPNEF
                     SGGQRQRIGIARAIALRPKLIVCDEPVSALDVSIQAQILNLLRDLQKEFGIAYLFVAH
                     DLAVVRQISHRVAVMYLGKIMETAPRELIYSRPLHPYTHSLLSAVPVPDPKRQAHRKT
                     RIVLHGDLPSPIDPPSGCVFRTRCFQAQERCAVEVPPLVELAPGHHVACHFPIAVEDL
                     PARAEVQAVAADAPHEAAPDVTTVSREEAADGAAGAAGKGRGAQEEALDEGGAAEEGV
                     SLNKPRS"
     gene            complement(3345..4433)
                     /locus_tag="VIN32_07255"
     CDS             complement(3345..4433)
                     /locus_tag="VIN32_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018851077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8922408.1"
                     /translation="MTATSELPVSGMPIAGSLDRPFLSVNDLHVAFPTEDGLVQAVDG
                     VSLSVERGKTLAIVGESGSGKSVTAQAIMGLVNRKSANVTGEIWLDGQELLHMPQGEV
                     QELMGMEIAMIFQDPMSSLHPFYRIGDQIVEAIRAHRSIGKAAARRETLEMLHHVGIP
                     AAERRIDAYPHQLSGGMRQRVMIAMALINSPQLLIADEPTTALDVTVQAQILELIARL
                     QGEFGTTVILITHDLGIVADVADDVAVMYGGRIVELASTTTLYDLPEMPYTLGLLSSV
                     PRMDQKLPERLDPIRGNPPSPIRLPKGCVFQPRCDYWQKVPGEACLTVRPELLESDPD
                     HWVRCHLKAEERKQIAHDVKQILAGGPE"
     gene            complement(4430..5422)
                     /locus_tag="VIN32_07260"
     CDS             complement(4430..5422)
                     /locus_tag="VIN32_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006592889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8922409.1"
                     /translation="MFAYLVRRVLLMLMLLFLLSVSVFLLFSILPGDPARLTCGKACT
                     PVIIEANRHRLGLDEPPLTQYVKFVSAIFVGRTFSPDSPEPIKCDAPCLGYSFNRHEQ
                     VMSLITRAAPVTFFLAIGAFVIWIFVGLSLGIVAALNRGRLGDRLIMGISLIGYSMPA
                     FFLGLLLVFFVIIKWQFLPFPQYISPWENPFQFFQTMLLPWILLATLYAAFYIRLTRN
                     QVLEVLSDDYVRTARAKGLPERTVVVKHALRAGLTPIVTAAGLDLAGLLGGAIISENI
                     FNLPGLGSLAVSSITDSDLPLITGITLLTAAIIIFANLVVDLLYAVIDPRVRVT"
     gene            complement(5526..7334)
                     /locus_tag="VIN32_07265"
     CDS             complement(5526..7334)
                     /locus_tag="VIN32_07265"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8922410.1"
                     /translation="MARLDVRVLRASIIGLAAAITLVACGSGNTTSPGGSGASAGPSG
                     SGAAGQKGGTIYILQSGDEWNQVDPQRAYTGEDLAFFGATIYRALVGYTVSPNDTEAS
                     KLVPDLATDTGTPNADASSWKFTLKDGVKWQDGSPITCDDVKYGVSRTFANDIINQGP
                     TYAVNYLDIPANPVTDAKDPKSVFLSAYYGPYDGTGQELFDKAVTCDGATITFNLGGP
                     HPDFNYTTTLGFSPVPKAADKGETYGVKAPPMSSGPYKVDSYTTGNGGKMILSRNDNW
                     DPKTDTYRTALPDSWEVDFGLDPKVIDQRLIASSGNDVTAIEYGGIQPENLAVVFSDP
                     KTPQSQFAGRAVSGYDIYALYYWINVQKVPNVKIRQAMAVALDRDAIRKNAGGDFVGD
                     FADGVIKPNIGPDYAATGMWTDMFGAAVPDTGDPELAKQLIAASGVAAPKLSYNFADT
                     PTGQKNAAIVISSLGKAGITVTPAPLERGKYYSVVFDPKSAGDFGTGGWGADWPNAST
                     VVPPLFTQAGGWDLSQVDDPAFNKKVDAAIAETDRTAQNALWQALNKEAMQNVYVIPT
                     FFELTQRLAGNKINSDGVFIWGPYGSWPYGALSVNP"
     gene            complement(7464..>8367)
                     /locus_tag="VIN32_07270"
     CDS             complement(7464..>8367)
                     /locus_tag="VIN32_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020384904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8922411.1"
                     /translation="IWARLRRDKVAMICLVVLVLFYLIGIFGPVVFGAFGLNPYTFDS
                     AAIGNSGGKPVADWGGITAAHPLGVEWGTGRDIMAQLIYGLRISLVIATSATMVTVTI
                     GTVVGIVAGYSGGWTDTIIGRLMDLVLAFPFLLIVLALSGVLTQRLTQLGVPDGNPSR
                     ILYLILVLSVFGWPYLARIVRGQVLSLREREFVESAVAMGSSKRRILFTEILPNLWAP
                     ILVYATLTLPAYIGTEAALSFLGVGVLPPETTFGAMLANSVTYFNVVPTYLFIPGTAL
                     VILVVSFNLLGDSLRDALDPKAIH"
BASE COUNT         1446 a         2684 c         2874 g         1363 t
ORIGIN      
        1 agacgaaccc gagcgactcg accccagccg ccgcgatcgc cgcggtcacc gccgacggcc
       61 cgggcagcac ctcgaccgag gccccggccg cccttgccgc ctcgacgagc cgcgcaccgg
      121 gatccgagac gccgggcatc ccggcatcgg tcgtgaacgc gagcgtggct cccccggcca
      181 ggcgatccag gagctcgctc ttccgcgtct cgggcgaatg gtcgttgtag ctgaccgtcg
      241 gccgcttcgc accaaggtgc gccaggaggc ggcgtgccat ccgggtgtcc tccgcgacca
      301 cgaggtcggc cgtgcgcagt gcctctgccg cacgcggggt gaggtccccc aggttgccga
      361 tcggggtccc taccaccagg aggcgtccag ccacgatcct ctcagctccc ccgccgcgag
      421 ccgggccggc ggcgcgggta gagatggtcg atcccggggg ttcgcaggct gaccttggcg
      481 accggcgggg tctggcgctt gaactcgttg cgcgccacgc gctcagccac ccaggccacc
      541 agccgtgcat ccatccccgc cgcgacgcag cgctcgaccc tccatcgccg gtcgacgagc
      601 gcgaacagga ttcgatccag gtcgtcgtag ctggcaccca gctcggcctc atccgtctgg
      661 tccggccaca ggtcggctga cggtggcttg gcaaggatct cggccggcac cccaagcgca
      721 gcggccacgg cgcgcagctg gctcttgtac aggtcgccga tcggcgccag ggcgctggcc
      781 atgtcgccgt gcagcgtgcc gtagccgagc agtgcctcgg tcttgttgct cgtccccacc
      841 accagggcgt cgaaggcggc ggatcggtcg tacaacacga tcatccgctg ccgggccatc
      901 acattccccc tgcgcacccg gagcgcagcg tcgtcatcgc cgctgataag ggcgagcatc
      961 ggctcgacca tgggcgtgat gtcgacccgc tcggtgcgac agcccagggc agccaccacg
     1021 cgcatggcat cggtctccga gtcgggtgaa gagctgcggt agggcatgcg gacggccagc
     1081 aagttgtcgg cgccgatcgc acgcgcggcg aggaaggcga cggtcgccga atcgacccct
     1141 ccggaaagcc cgaccaccaa ccgccggaag ccggtctgct cggcctggga acggatgaag
     1201 gcggtgatca cctccagcgc ggagggcgcg tcgatctccg ggagaggagc cgtcacgcgt
     1261 catcctggtc gggcggtgac ggcagcccgg cacgccccgc gatgatccgg tccagctcac
     1321 gacggacgag ctcgaggcgc tcgtcggaga ggagcggaag gccatagcgc tgcgtgcgca
     1381 ggtcgtcgct gtcgatcgag ccaaccacca gagcctcctc gtacaacggg ccctgggcga
     1441 tggtcgagcc atcggctccg agcaggcgtg agccgcccca gaaggtcagc ccctcctcgt
     1501 tgccgacccg gttgcagaac gcgaccgcca ccgtgccgag cagcgcatag gtgtcctgca
     1561 tcttgtgcca gccggcaatc gccgccagcc cagccgagct gccgggggcg cgcgccggac
     1621 ccgcggccag gttcacgagc aggctggcac cgtcgagcgc ctggagcatc ggcaggctgg
     1681 catgccagaa gtcctcgcag atgctgaggc cgatgctgcc gagtggctcc ccgactgcat
     1741 gcgtccggat ccgatccccc gcccgcgtga agcgcccctc gtcgaacatg ccgtaggtcg
     1801 gcaggtagac cttgcggtgc agcccgatca gctcgccatc tctcagcagc gcggcgctgt
     1861 tgcagaagcg gtgcgcatcg gtctcctcga cgaagccgat ggccagcacc accccgggcg
     1921 cctcgcggct cagcgcggcg aggcgcgggt cgtccgcccg catggcgaca tcaggcacca
     1981 ggtcggagag catgtagccg gtcagtgcga gctcgggaaa ggcgacgagc tccgccccct
     2041 cggtcatggc tcggcgcgac cagcccgcag ccaattcgag atttgcaccc acgtcgccca
     2101 ggcgcggggc gtattgcgcc agcccgatgc ggtactccac gcgagggctc aggaccgggg
     2161 cttgttcaga ctgacgccct cttcggcggc gcccccctca tcgagagcct cttcctgagc
     2221 gcccctcccc ttgccggccg cccctgcagc tccgtcggcg gcttcttccc gtgaaaccgt
     2281 cgtgacatcg ggggcggcct cgtgcggagc gtcggcagcc accgcctgaa cctcggccct
     2341 ggccggcagg tcctcgacgg cgatcgggaa gtgacacgcc acatggtggc cgggtgccag
     2401 ctcgacgagc ggaggaacct cgacggcgca gcgctcctgc gcctggaagc agcgcgtccg
     2461 gaagacacag ccgctcggcg gatcgatcgg actcggcagg tcgccgtgca gcacgatccg
     2521 cgtcttgcga tgtgcctgtc gttttggatc cggaaccggc acggccgaca ggagcgagtg
     2581 ggtgtagggg tgaagcggcc tggagtagat cagctcgcgg ggtgccgtct ccatgatctt
     2641 gccgaggtac atgacggcga ctcggtgcga gatctgcctg acgaccgcga ggtcgtgggc
     2701 cacgaacagg tacgcgatcc cgaactcctt ctggaggtcc ctcaggaggt tgaggatctg
     2761 ggcctggatc gagacgtcga gcgccgagac gggctcgtcg cagacgatca gcttcggccg
     2821 gagcgcaatc gccctggcga tgccgatgcg ctggcgctgg ccgcctgaaa actcattcgg
     2881 atagcggttg tagtgctcgg ggttcagccc tacccgctcc atcagctcca ggaccccctg
     2941 cttgacgcca ttcggaggcg tcaccttctg gaccttgaac ggggcgctga tgatggcgcc
     3001 gacggtgtgg cgcgggttca gggcgttgta cggatcctgg aagatcatct gcgccttgcg
     3061 ccggatcggc atgaggttcc gcgcggacag atccgtgatc tcctcgccat cgatcttgac
     3121 gctgccgccg gtgggacgaa cgagcatcag gatcgacctg gccgcggtcg tcttccccga
     3181 gccgctctcg ccgacgagcc cgagggtctc cccggcgtcg aggctcaggc tgaccccgtc
     3241 gaccgccttc accgcgccga ccgtgcgtga gatgagtccc cgtgacttga cgggaaagta
     3301 ggtccgaagg tcggtgacgt cgaggacatc gctcgtccgg gtcgtcactc cgggcccccg
     3361 gccaggatct gcttcacgtc atgcgcgatc tgcttgcgct cctcggcctt caggtgacag
     3421 cgcacccagt ggtcaggatc ggactcgagg agctcgggtc gaacggtcag acaggcttcg
     3481 ccggggacct tctgccaata gtcacagcgc ggctggaaga cgcagccctt ggggagcctg
     3541 atcggcgacg gtgggtttcc gcgaatcggg tcgagccgct cgggaagctt ctgatccatt
     3601 cggggaacag atgagaggag ccccagcgtg tagggcatct cgggcagatc gtagagcgtc
     3661 gtggtcgagg ccagctcgac gatccggccg ccgtacatca ccgcgacgtc gtcggcaacg
     3721 tccgcgacga tcccgaggtc atgggtgatc aggatgacgg tggtcccgaa ctcgccctgc
     3781 aggcgcgcga tgagctcgag gatctgggcc tggacggtga catcaagggc cgtcgtcggc
     3841 tcgtcggcga tcaggagctg cggggagttg atcagagcca tggcgatcat gacgcgctgg
     3901 cgcatcccac ccgagagctg gtgcgggtag gcgtcgatgc gccgctcggc cgccgggatc
     3961 ccgacgtggt gcagcatctc cagcgtttcg cgacgcgccg ccgcctttcc gatcgaccgg
     4021 tgggcacgga tcgcctcgac gatctggtca ccgatccgat agaacggatg gaggctcgac
     4081 atcgggtcct ggaagatcat ggcgatctcc atgcccatca gctcctggac ctcgccctgc
     4141 ggcatgtgga ggagctcctg tccgtcgagc cagatctcgc cggtgacatt tgcggacttc
     4201 cggttgacga ggcccatgat cgcctgcgcg gtgacgctct tgcccgagcc cgactcgccg
     4261 acgatcgcaa gcgtcttgcc gcgctccacg gagagggaga cgccgtcgac cgcctggacg
     4321 agtccgtcct cggtcgggaa ggcgacgtgc aggtcgttga cggagaggaa cggccggtcg
     4381 agcgagccgg cgatcggcat gccgctgaca ggcagttcgc tggtcgctgt catgtgacgc
     4441 gcacccgcgg gtcgatcacc gcgtagagca ggtcaacgac caggttggcg aagatgatga
     4501 tcgcagccgt caggagtgtg atcccggtga tcaggggcag gtctgaatcg gtgatcgaac
     4561 tgacggcgag cgaaccgagc cccggaaggt tgaagatgtt ctccgagatg atggcgccgc
     4621 cgaggaggcc ggcgaggtcg agcccggccg ccgtgacgat cggcgtcagt cccgctcgca
     4681 acgcgtgctt gaccacgacc gtgcgctcgg gcaggccctt tgcgcgcgcg gtgcgtacgt
     4741 agtcgtcgct caggacctcg agcacctggt tgcgagtgag acggatgtag aaggctgcgt
     4801 acagggtcgc cagcaggatc cagggcagga gcatggtctg gaagaactgg aacgggttct
     4861 cccagggaga tatgtactgg gggaacggca ggaattgcca cttgatgatg acgaagaaga
     4921 ccaggaggag cccgaggaag aacgccggca tcgaatagcc gatgagcgag atgcccatga
     4981 tcagccggtc gcccaggcgt ccacggttga gggcggcaac gatgcccagg ctcaggccca
     5041 cgaagatcca gatgacgaag gcgccgatcg cgaggaagaa ggtgactggc gccgctcgcg
     5101 ttatcagcga catgacctgc tcatgccggt tgaaggagta gccgagacag ggggcatcgc
     5161 acttgatcgg ctcgggcgag tcgggggaga aggtgcggcc gacgaagatc gccgacacga
     5221 acttgacgta ctgggtcagg ggaggctcgt ccaggccaag gcggtgccgg ttcgcctcaa
     5281 tgatgaccgg ggtgcacgcc ttgccacagg tcagacgagc agggtcgccc ggcaggatgc
     5341 tgaagagcag gaagacgctg acgctcagca ggaatagcag catgagcatc aacaggactc
     5401 ggcgaaccaa gtatgcgaac acgcagcctg ccctccgtca gcgaaaggtt gcgtccccgg
     5461 aggagcggca tcgccactcc cccggggacc tgagcgctag ttcctaaact gacgctccgt
     5521 cgtgcctacg ggttgacgga gagggcaccg tagggccacg agccgtacgg gccccagatg
     5581 aagacaccat ctgagttgat cttgtttcca gcaaggcgct gggtcaactc gaagaacgtc
     5641 gggatgacgt agacgttctg catggcttcc ttgttgagcg cctgccacag tgcgttctgc
     5701 gcagtgcgat cggtctcggc aatggccgca tcgaccttct tgttgaaggc cgggtcgtca
     5761 acctgggaga gatcccagcc accggcctgc gtgaagagcg gcgggacgac ggtggaagcg
     5821 ttcggccagt ccgcgcccca accgcctgtt ccgaagtcac cggcactctt ggggtcgaag
     5881 acgacgctgt agtacttgcc gcgctcgagt ggggccggcg tcacggtgat gcctgccttg
     5941 cccagcgagg agatgacgat tgcagcgttc ttctgaccag tcggggtatc ggcgaagttg
     6001 tagctgagct tcggagccgc cacgcctgaa gcggcaatca gctgcttcgc aagctccggg
     6061 tcaccggtgt ccggcaccgc tgccccgaac atgtcggtcc acatcccggt cgcggcatag
     6121 tcaggaccga tgttcggctt gatcacgcca tcggcaaagt cgccgacgaa gtcaccacct
     6181 gcattcttgc ggatggcatc gcggtcaagg gcgaccgcca tggcctgacg gatcttgacg
     6241 ttcggaacct tctggacatt gatccagtag tagagggcgt agatgtcgta gccgctgact
     6301 gcgcggcctg cgaactgaga ctgcggagtc ttcgggtccg agaacactac ggccaggttc
     6361 tcaggctgga tgccgccgta ctcgatggcg gtcacgtcgt tgcccgagct cgcgatgagc
     6421 cgctggtcga tgaccttcgg gtcgagaccg aagtcaacct cccaactgtc ggggagggca
     6481 gtgcggtacg tgtcagtctt cgggtcccaa ttgtcgttgc gggaaaggat catcttgcca
     6541 ccgttgccgg tggtgtagct gtcgaccttg tagggtcccg aggacatcgg cggcgcctta
     6601 acaccatagg tctcgccctt gtcggcggcc ttggggaccg gcgagaaacc gagcgtggtg
     6661 gtgtagttga agtcggggtg cggcccgccc aggttgaacg tgatggtggc cccgtcacag
     6721 gtgaccgcct tgtcgaacag ctcctgtccg gtgccgtcgt acggaccgta gtaggccgac
     6781 aggaacactg actttggatc cttcgcgtca gtgaccgggt tggccgggat gtcgaggtaa
     6841 ttgactgcgt atgtcgggcc ctggttgatg atgtcgttcg cgaaggttcg cgagacaccg
     6901 tacttgacgt catcgcacgt gatcggcgag ccgtcctgcc acttgacgcc atccttgagg
     6961 gtgaacttcc acgacgaagc gtcggcgttc ggcgttccgg tgtcggtcgc cagatccgga
     7021 acgagcttgg atgcttcggt gtcgttcggt gagaccgtgt agcccacgag cgcccgatag
     7081 atcgtcgcgc cgaagaaggc gaggtcctca ccggtgtatg cgcgctgcgg atctacctgg
     7141 ttccattcgt cgcctgactg caggatatag atcgtgccgc ccttctgacc ggcggccccc
     7201 gatccgctcg ggccggcact cgcgccggac cctccggggc tcgtcgtgtt gcctgaaccg
     7261 caggcaacca gcgtgatcgc ggctgccagg ccaatgatcg aggcacggag cacccgtaca
     7321 tctaatctcg ccatgttcca tcctccaacg agttgtgccc gggaaggttc cccggcgcaa
     7381 gttgatgccc gatcatacac gctgtcgagc agtgggttcg aaagtgcggg cggctcctac
     7441 ctcgccggca ccttgacggg gattcagtga atggcctttg gatcgagggc gtcgcgaagc
     7501 gaatccccca gcaggttgaa cgagacaacc aggatgacga gggccgtgcc cgggatgaac
     7561 aggtaggtcg ggacgacatt gaaataggtc acggagttgg cgagcatcgc accgaaggtc
     7621 gtctcgggcg ggagtacgcc aacgccgagg aatgagagcg cagcctcggt cccgatgtac
     7681 gccggcagcg tgagcgtcgc atacaccagg atcggcgccc acaggttcgg gaggatctcg
     7741 gtgaacagga tcctgcgctt gctcgacccc atcgcgactg ccgattcgac gaactcgcgc
     7801 tcccgcaggc tgaggacctg cccgcgcacg atgcgggcca ggtacggcca gccgaagacg
     7861 ctcagaacga gaatcaggta gaggatgcgc gatgggtttc cgtcagggac gcccagctgg
     7921 gtgaggcgct gggtgaggac cccggagagg gccagcacga tgagcaggaa tgggaaggcg
     7981 agcaccaggt ccatcaggcg cccgatgatg gtgtcggtcc agcccccgct gtagccggcg
     8041 acgatgccga cgacggtccc gatcgtgacc gtgaccatgg tcgcgctggt cgcgatgacc
     8101 agggagatcc gcaggccata gatcagctgg gccatgatgt ctcgcccggt cccccactcc
     8161 accccgagtg ggtgggcggc ggtgatgccg ccccagtccg cgacgggctt accccccgag
     8221 ttgccgatcg cggcgctgtc gaacgtgtac ggattgaggc cgaacgcacc gaagacgacg
     8281 ggaccgaaga tcccgatgag gtagaagagc accaggacca ccaggcagat catcgcgacc
     8341 ttgtcgcgac ggagacgcgc ccagatg
//
LOCUS       DATCQR010000218         6285 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_27513, whole genome shotgun sequence.
ACCESSION   DATCQR010000218 DATCQR010000000
VERSION     DATCQR010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6285
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27513"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..468)
                     /locus_tag="VIN32_07275"
     CDS             complement(<1..468)
                     /locus_tag="VIN32_07275"
                     /inference="COORDINATES: protein motif:HMM:NF013918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HEY8922412.1"
                     /translation="MAPGHLAVTIDHTLSWEIARVGGLLAYILTTVSVVLGLLLSLKA
                     YSPRWPRFITNELHRYVTVLSLVFIAVHSLAIWVDPFTGFNLGEVLLPMASHYRPLWI
                     AFGIVGAYLAVAIWLSEYVRSSVGYAWWHRFHMLAFVVFLLGTVHGLASGSDST"
     gene            complement(444..1367)
                     /locus_tag="VIN32_07280"
     CDS             complement(444..1367)
                     /locus_tag="VIN32_07280"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEY8922413.1"
                     /translation="MGPEAVVAGRRAGMVEHRFRSMGCEVAVLAPSSRPDAEQLTQEV
                     FARWDARFSRFRPDSELEGLNNAGGRPFRASAPMLTVLGAALRAARATDGLFDPLLGG
                     RMAELGYDRTYEQLPAKRSASTLRDWRAGAWREIVVDAEHETVQLPLGYRVDLGGIAK
                     GMAVDAAIEAMVIAGVPDVAVSAGGDLAVSGLPPGQESWMVAIEDTPAETVVAIRQGA
                     LATSSVRRRRWVVNDAERHHLLDPRTGLPATGPIVQASVAAATCGQAEVAAKMAILSD
                     LPGAIGRLEQHRLAALLVMSNGEAWRLGTWQ"
     gene            complement(1357..1626)
                     /locus_tag="VIN32_07285"
     CDS             complement(1357..1626)
                     /locus_tag="VIN32_07285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8922414.1"
                     /translation="MIRSQDVRGDMRRTALEQRVYRLKVAALAVAAGASLALWALVSG
                     AVAGTAAAPSMPAASGEQSQATDLFGNGSTLGVGSGTPVIRSHGS"
     gene            complement(1701..2624)
                     /locus_tag="VIN32_07290"
     CDS             complement(1701..2624)
                     /locus_tag="VIN32_07290"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HEY8922415.1"
                     /translation="MSNPTFLDLPSTALEAIGAARADVAILGIPFGVPYPAPGMTAGC
                     ADAPAAIRTRSRRLANFADHHDFDLDAPMLVGSPPMRIVDAGDVPGLADDGAGNSSRA
                     ESAVRAVLGAGAVPIVLGGDDSVPIPVLRAYAGGTPLTILQIDAHLDFRDEVGGIRDG
                     YSSPMRRASEMDHVERIVQVGLRGVGSARPGDVADARAAGNVLVTARELREGGVPWLL
                     EQLPQGASLFIVVDLDGLDPSVAPAVSGSSPGGLSYDEVGDLLGGATARCRVVGAAFT
                     ELVPALDVDGRSALVVVRMVMRLLAGLDRNR"
     gene            complement(2621..3082)
                     /locus_tag="VIN32_07295"
     CDS             complement(2621..3082)
                     /locus_tag="VIN32_07295"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HEY8922416.1"
                     /translation="MSSSTPLPEPWASVRQRLRERGLRWTTQRRAVVEALAGSDGHIT
                     GTELIERCRTIDPATIPSTVYRTLDVLEELGMVQHGHGADGREEFHVGPEGVHGHLHC
                     ATCGGRWEIRAGAARSIVDALRSSDGFEVDLSHVTVVGRCADCRASAAAAQ"
     gene            3081..3548
                     /locus_tag="VIN32_07300"
     CDS             3081..3548
                     /locus_tag="VIN32_07300"
                     /inference="COORDINATES: protein motif:HMM:NF040028.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6220 domain-containing protein"
                     /protein_id="HEY8922417.1"
                     /translation="MGTVLSAGAVRALIAVLGGWFEMVSGARYVFVTLVWIYLAGILV
                     QVFLAGIGLFGAAKDFEPHVGLGWILHLVPVLLLIAAAVGRVGRGLLWWTAALLVVQF
                     VQPILATLRNDLPLVAAFHPVLALIIFWLAVMIGLKALRLVREAAPTLARPEP"
     gene            3558..4049
                     /locus_tag="VIN32_07305"
     CDS             3558..4049
                     /locus_tag="VIN32_07305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8922418.1"
                     /translation="MDWQPWLIFAHILGAFTFVLAHGVSIFAALRLRSERDQARVGAL
                     LDLSKSAVTIAAIAVAFLLLTGIAAGFAGNYWGHRWIWASIAILVLLWGYMGFRGTMY
                     HDALRRAVGSVGIYDHKAAEAPPPDPAALTVLLASSRALELAAVGGIGLVVILWLMVF
                     KPF"
     gene            complement(4139..4918)
                     /locus_tag="VIN32_07310"
     CDS             complement(4139..4918)
                     /locus_tag="VIN32_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005268066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEY8922419.1"
                     /translation="MDEEVFRALADTSRRALLDALRERDGQALSELEKALPGMTRFGV
                     MKHLRVLADAGLLTTRRDGRRKIHYLNAVPIRLIHDRWISRYAEPWAAALSDLKTRLE
                     SPMEKVFEIYIKTTPERLWHAITDPEIRSKYHFGNRISSDWKPGGRFEMGHQNAPGLL
                     GEGENLEVDPPRRLVQSMVALWGEDVKSEGTSRITWEIEPVGDSCRLVVTHDQLHEGA
                     NDQLYGGWPMILSGLKTWLETGELLTTPGSLMYATPSESAR"
     gene            5030..5419
                     /locus_tag="VIN32_07315"
     CDS             5030..5419
                     /locus_tag="VIN32_07315"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY8922420.1"
                     /translation="MSDDENRATLERTITAMFAGDIDASTASMADDAVVEWPQSGERI
                     VGRQACSLVYKNYPGGSPSYELRRISGSGDLFVVEAVGNYGADTSYMTSIVEFRDGQI
                     VKQTDYFASPFEAPVWRSQCVERMEPA"
     gene            complement(5426..5845)
                     /locus_tag="VIN32_07320"
     CDS             complement(5426..5845)
                     /locus_tag="VIN32_07320"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY8922421.1"
                     /translation="MASHDSPLAAALERVGDRWSLLLVEALLPGPRRFNELGEAVLGI
                     APNILADRLRRLESERIVSATPYSERPPRFAYALTDEGQELAGALRLLADWGSRVSRE
                     SEPLRHAACGTPVEARWYCPTCDRPVEGKEAEQVRRV"
     gene            5938..>6285
                     /locus_tag="VIN32_07325"
     CDS             5938..>6285
                     /locus_tag="VIN32_07325"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEY8922422.1"
                     /translation="MGPMDELGKAIASVASTAGASTVGIGNRWRGGSGVVVESGKVLT
                     NAHNLHGDEVHVFFADGRDAEGKVLGADGDGDLAVIGVDTGDAPAIEWGNGSALSIGS
                     PLVALGNPAGNGLR"
BASE COUNT         1018 a         2129 c         2169 g          969 t
ORIGIN      
        1 ggttgaatcg ctgccgctcg ccagtccgtg aaccgtcccg agcaggaaca cgacgaaggc
       61 gagcatgtgg aagcggtgcc accaggcata cccgacggag ctgcgcacgt actccgacag
      121 ccagatcgcg accgccaggt aagcgccgac gatgccgaac gcgatccaga ggggccgata
      181 gtgcgaggcc atcggcagga gcacctcgcc caggttgaag ccggtgaacg gatcgaccca
      241 gatcgccagc gagtggaccg caatgaacac gagggagagc accgtgacgt agcgatgcag
      301 ctcgttggtg atgaagcgcg gccatcgggg cgaatacgcc ttcagcgaga ggaggagccc
      361 gagcaccacc gagacggttg tcaggatgta cgccagcagg ccaccaacgc gggcaatctc
      421 ccatgacagg gtgtgatcga tggtcactgc caggtgccca ggcgccacgc ctcgccattc
      481 gacatgacca acagcgccgc gagccgatgc tgctcgagtc gaccgatcgc gccgggcagg
      541 tcggacagga tcgccatctt ggcggcaacc tccgcctggc cgcaggtcgc cgcggcgacg
      601 gacgcctgca cgatcggtcc agtcgcgggc aaaccggtcc gcgggtcgag gaggtgatgc
      661 cgttcggcgt cgttcaccac ccacctgcgc cgccggaccg atgacgtggc cagggcgccc
      721 tgacggatgg caaccaccgt ctccgccggg gtgtcctcga tggcgaccat ccaggactcc
      781 tgtccgggcg gaaggccaga gaccgccagg tcaccacccg cgctgactgc gacatccggg
      841 acgccggcaa tcaccatcgc ttcaatcgcg gcatccacgg ccatcccctt ggcgatgccc
      901 cccagatcga cccgataccc gagcggcagc tggacggtct cgtgctcggc gtccaccacg
      961 atctcgcgcc acgccccggc tcgccaatcg cgaagcgtgg acgcggaccg cttcgccggc
     1021 aactgctcgt aggtgcggtc gtacccgagc tcggccatgc ggccgccgag gagcggatcg
     1081 aacagcccgt ccgtcgctcg cgctgcccgc agcgcggcgc cgaggaccgt cagcatcggc
     1141 gctgaagcgc ggaagggtcg gccgcccgcg ttgttgagtc cctccagctc ggaatcgggt
     1201 cggaagcggc tgaagcgcgc atcccagcgc gcaaagacct cctgggtcaa ttgctccgcg
     1261 tcaggacggc tggacggcgc cagcacggcc acttcgcagc ccatcgaccg gaagcgatgc
     1321 tcgaccatgc cggcgcgccg gccggcgaca accgcctcag gacccatgcg aacggatgac
     1381 cggcgtaccc gagccgacgc cgagggtcga gccatttccg aacaggtcgg tcgcctggct
     1441 ctgctcaccg ctggctgccg gcatcgatgg tgccgccgcc gtgccggcca cggccccgct
     1501 gaccagggcc cacagcgcaa gcgacgcgcc tgcagcgacg gccaaggcag cgaccttcag
     1561 gcggtaaacg cgctgctcca gcgcggtgcg acgcatgtca ccgcggacat cctgggatcg
     1621 gatcatgccg ggaggttacc ggcaaggtgt tgcgatctgc ccgcccgcag ggataaggct
     1681 cgttagcctc ctgtcggcga tcagcggttg cggtcgaggc ccgccaacag ccgcatcacc
     1741 atccgaacca ccaccagcgc cgaccggccg tcgacgtcga gcgcgggcac gagctcggtg
     1801 aaggccgcgc cgaccacacg gcatcgtgcc gtcgcgccac ccaggaggtc ccccacctcg
     1861 tcgtaggaca ggccaccggg cgagctgccg ctcaccgcgg gtgcgacgga ggggtcgagc
     1921 ccatcgaggt cgacgacgat gaagagagac gccccctgcg gcagctgctc cagcagccac
     1981 ggcactcccc cttctcgaag ctcgcgggcg gtgacgagca cgttgccggc tgcccgggca
     2041 tcggccacat ctccagggcg ggcgctgccg acgccgcgca ggccaacctg cacgatccgc
     2101 tcgacgtggt ccatctcgga cgcccgccgc atcggcgacg agtagccgtc tcgaatccca
     2161 ccgacctcgt cacggaagtc aaggtgcgcg tcaatctgca ggatggtgag gggagtccca
     2221 ccggcatagg cacgcaggac ggggatcggc accgaatcgt cgccgccgag cacgatcggg
     2281 acggcgcctg cccccaggac cgcccgaaca gcggattccg cgcgagacga gttgcccgcg
     2341 ccgtcatcag cgaggccggg aacgtcgccg gcgtcgacga tgcgcattgg tggcgaaccg
     2401 accagcatcg gtgcgtcgag gtcgaagtcg tgatggtcgg caaaattcgc gagccgacgg
     2461 gagcgcgtcc ggatggccgc cggcgcgtcg gcgcagccgg ccgtcatgcc cggcgcggga
     2521 tagggcacgc cgaaggggat ccccaggatt gcgacgtcgg cgcgcgcggc gccaatcgcc
     2581 tccagcgccg tggacggtag gtccaggaat gtggggttgc tcattgggcg gctgcggccg
     2641 aggcgcggca gtccgcgcag cggcctacca cggtcacgtg cgagaggtcc acctcgaagc
     2701 cgtccgacga gcgcagggca tcgacgatcg agcgagccgc tccggcgcga atctcccagc
     2761 gcccgccgca ggttgcgcag tgcaggtggc cgtgcacccc ttcggggccg acgtgaaact
     2821 cttcgcgccc atcggcgccg tgcccgtgct gcaccatgcc gagctcctcg agcacgtcga
     2881 gcgtgcgata gacggtcgag gggatcgtgg ccggatcgat cgtccgacag cgttcgatca
     2941 gctcggtgcc ggtgatgtgc ccgtcgctgc cggccagcgc ctcgaccacg gcccgtcgct
     3001 gcgtggtcca gcgcaagccg cgctcgcgga gccgctgccg gacgctggcc cagggctcgg
     3061 ggaggggcgt ggagctcgac atgggcacag tattatctgc cggcgctgtg cgggccctga
     3121 tcgcagtgct tggagggtgg ttcgagatgg tgtccggagc ccgatacgtc ttcgttaccc
     3181 tggtctggat ctacctggcc gggatcctgg tgcaggtctt cctggcgggg atcggcctgt
     3241 tcggggccgc gaaggatttc gagccgcacg tcggcctggg ctggatcctg cacctcgtgc
     3301 cggtcctact gctgatcgcg gctgctgttg gtcgtgtcgg acgcggcctc ctgtggtgga
     3361 ccgccgcgct actggtggtg cagttcgtcc agccgatcct ggcaaccctg cgcaatgacc
     3421 tgccgctggt cgcggccttc caccccgtgc tggcgctgat catcttctgg ctcgcagtca
     3481 tgatcggcct caaggcacta cggctcgtcc gagaggcggc gccgactctc gcacggccag
     3541 aaccgtagga ggggccgatg gactggcagc cgtggctcat cttcgcccac atcctcggtg
     3601 ccttcacctt cgtcctggcg catggggtct ccatcttcgc ggcgcttcgg cttcgcagcg
     3661 agcgtgacca ggcccgcgtc ggggcactcc tcgacctctc gaagtcggcc gtgacgatcg
     3721 cggccatcgc ggtcgccttc ctcctgctca ccggcatcgc cgccggcttc gcgggcaact
     3781 actgggggca tcgctggata tgggcatcga ttgcgatcct ggtcctgctg tggggctaca
     3841 tgggcttccg cgggaccatg taccacgacg cattgcgccg cgcagtcggc agcgtcggca
     3901 tctacgacca caaggcagcc gaggcgccgc cgcccgaccc ggcggcgctg actgtgctcc
     3961 tggcatcatc gcgagccctg gaactcgcgg cggtcggagg catcggcctg gtcgtgatcc
     4021 tttggctgat ggtcttcaag ccgttctagg cggcagccgc catctcttca agtccgaacg
     4081 agtcgttggg gcggcctggt gctgatgcgc cgcagctcgc ctcgcgccgc gttgatcgtc
     4141 atcgagccga ttccgagggc gtggcataca tcagcgaacc tggggtcgtg agcagctcgc
     4201 cggtctcgag ccatgtcttc aggccggaga ggatcatcgg ccagccgccg tacagctggt
     4261 cgttcgcgcc ctcgtgcagc tggtcatggg tcacgaccag gcggcatgag tcaccgaccg
     4321 gctcgatctc ccaggtgatc cgcgaggttc cctcgctctt cacgtcctcg ccccagagcg
     4381 cgaccatgct ctgcaccagg cgtcgcggcg ggtcgacttc gaggttctcg ccctcgccca
     4441 gcaggccagg ggcgttctga tggcccatct cgaaccggcc tccgggcttc cagtcggagc
     4501 tgatccggtt gccgaagtgg tacttgctgc ggatctcggg atcggtgatc gcatgccaca
     4561 ggcgctccgg cgtcgtcttg atgtagatct cgaacacctt ttccatgggg ctctccagtc
     4621 ttgtcttgag gtcgctcaac gcagccgccc agggctcggc gtagcggctg atccatcggt
     4681 cgtggatgag ccggatcggg acggcgttca ggtagtggat cttccgacgc ccatcgcgtc
     4741 gggtggtgag caggcccgcg tctgcgagca cgcgcaggtg cttcatgacg ccgaaccgag
     4801 tcatacccgg cagcgccttc tccagctcgg agagtgcctg tccgtcgcgt tcgcgaagcg
     4861 cgtcgagcag ggcacggcgg ctggtatcgg cgagggctcg gaagacttcc tcgtccatgc
     4921 ccgcgactat atgtgaccga ttagtcacct gtcaagcagg acacgcctcg acaggctcga
     4981 gtgggaggca cagcgtgatg gtgttcagcg agcgaaggag agggagccaa tgagcgacga
     5041 cgaaaacagg gcgacgctgg agcgcacgat caccgcgatg ttcgccgggg acatcgacgc
     5101 atcgacggct tcgatggccg acgacgcggt ggtggagtgg ccgcagtcgg gcgagcggat
     5161 cgtcggcagg caggcctgca gcctggtcta caagaactac ccggggggat cgccctcgta
     5221 cgagctgcgg aggatcagcg ggtctgggga cctgttcgtg gtcgaagctg tcggcaacta
     5281 cggcgccgat acgagctaca tgaccagcat cgtcgagttc cgcgacggcc agatcgtcaa
     5341 gcagacggac tacttcgcca gcccgttcga ggcccctgtc tggcgttcgc agtgcgtcga
     5401 gcggatggag cccgcctagc cagcgctaga cccggcggac ctgctcagct tccttgccct
     5461 caacggggcg atcacaggtc gggcaatacc agcgggcctc gaccggcgtg ccgcaggccg
     5521 cgtgtcgcag cggctcggat tcgcgggaga cgcgtgagcc ccagtcggcg agaaggcgca
     5581 gtgcaccggc cagctcctgg ccctcgtcgg tgagcgcgta ggcgaagcgc ggcggacgct
     5641 ccgagtaggg agtcgcggac acgatccgct ccgactccag gcgccgcagc cggtcggcca
     5701 ggatgttcgg cgcgatcccg aggacggcct cgcccagctc attgaagcgg cgtgggcctg
     5761 gcaggagcgc ctcgaccagc agcaggctcc atcggtcacc cacccgttcc agcgcggcgg
     5821 cgaggggtga gtcgtgggag gccatgacgg gatcgtaagg catcggcctt gacaaacctt
     5881 ccacggggag taacttgcgc attgcaagtc acacgctacc agatggagaa cagaccgatg
     5941 ggtcctatgg atgagcttgg caaggcgatc gcttcggtcg catcgaccgc gggcgcttcg
     6001 acggtcggga tcggcaaccg atggcgcggc gggtccggcg ttgtggtcga atccggcaag
     6061 gtgctcacca acgcgcacaa cctgcacggc gacgaggtgc atgtcttctt cgccgatgga
     6121 cgcgacgccg aggggaaggt cctcggagcc gatggcgacg gcgacctggc ggtgatcggc
     6181 gtcgataccg gagacgcacc agcgatcgag tggggaaacg ggtctgcgct gagcattggc
     6241 tcgccgcttg tcgcgctcgg taacccggcg ggcaacggcc ttcgc
//
LOCUS       DATCQR010000219         7719 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_12329, whole genome shotgun sequence.
ACCESSION   DATCQR010000219 DATCQR010000000
VERSION     DATCQR010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 7719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7719
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12329"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(19..414)
                     /locus_tag="VIN32_07330"
     CDS             complement(19..414)
                     /locus_tag="VIN32_07330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8922423.1"
                     /translation="MKRIVGAVLAGLMTFGLVFGMAASLNLTSDSLGSATTVVAACQA
                     GAMNATYASTYTAAAPGPGYTVGTVTITGLAATCYTKPYKVTLATAAGASLGEATGTT
                     PGAGTTIVVTFAPVVAAASVTAISVVISG"
     gene            complement(539..1744)
                     /locus_tag="VIN32_07335"
     CDS             complement(539..1744)
                     /locus_tag="VIN32_07335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8922424.1"
                     /translation="MDEIALRYLLLGLRLERHLPRLVASYHGPAELSEAVGGEPLTPA
                     AELHDEAMQLAGMAAELQADTAARRRRATWLIAQVGAMGALARWVGGEEIGYVDLVED
                     LYDIEVQLEPDATFDTARRMLDSALPGGATLRERLAEHDARSRVPPERAIALASELSA
                     RLRTRTRAQLWLPEGESLRIEAAHGVDWAIDAHYLGAGISVVRLNVDRPLTFATLAET
                     VAQEGYPGHHAEASVKDDLLASAGHEELTLTTTLSPQAVVSEGMAGIGREVVMSDQEL
                     GLELQRLARSVDRRLDVEAELMVQRARRLLSPALGNAAVALHRDGEPVEDVRSYLAEE
                     GLVGDESLDEVIWRLQDPLLRTQPLAQVEGRRLVSEWLEVHGQSHGFSRLLSEQQTPH
                     ALRSELNPT"
     gene            1967..2911
                     /gene="rsgA"
                     /locus_tag="VIN32_07340"
     CDS             1967..2911
                     /gene="rsgA"
                     /locus_tag="VIN32_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEY8922425.1"
                     /translation="MPTPVRAAAEAALTGIVLRARSGFYTVRLDDGTLLECRLRGRVK
                     QDRGNSDLVVIGDRVAVAPLEDGDGMIESVEPRRTRFSRRQPGPRGSWKEDMIVANLD
                     QVLVVFACADPMPHLRMVDRFLVVAEHNQVEAVLVANKVDLVGLPAARSLFAGYQEIG
                     YPVHYVSAREGIGLEELADRLVGRVSVVTGPSGVGKSTLLNAIQPGLRIETGAVSETV
                     HKGRHTTTSAELHPLTAAGGGYVADTPGLRELGLWQVPPEELAWCFPEMRDYIGKCGF
                     NDCRHISEPRCAVLAAVAAGRISTARHDSYRRLLLNEE"
     gene            2939..3640
                     /locus_tag="VIN32_07345"
     CDS             2939..3640
                     /locus_tag="VIN32_07345"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY8922426.1"
                     /translation="MEIAPGVHAVRLLSVYAFLIDEPQLTLIDAGLIGSAGRVQRHVE
                     RIGRSLDDLVRIICTHAHPDHIGGVRELAGERDVEVLMHTADLDGLSVGLRDAVANRN
                     RGQLIAYFTRHPGEATPIEDGQVLPILGGLRVVHTPGHTPGSICLYAERHKLLFVGDT
                     LQVIRGKVTYASRVFSEDLALARASVARMAELDVETIAFSHYPPLREGANEVLRGLAE
                     RAAAMSDRKRRAASL"
     gene            3793..6279
                     /locus_tag="VIN32_07350"
     CDS             3793..6279
                     /locus_tag="VIN32_07350"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY8922427.1"
                     /translation="MRESARRFADRPALLIKPGFRTRIWTYGDLADLAPKVASVLADQ
                     GLERGDRVLIWGVNRPEWSLGFFGALFAGLVLVPLDVRSQPEFVAKVAQRTRARLVLA
                     STQTASLAEGLNLPLLLIESLPDRARNVTPLPTPDIVASDVAEVVFTSGTTGEPKGAM
                     LTHGNLVANATALQDVFPFKPDERLLSILPLSHMFEQTCGLIAPFLVGASIVYPVSRQ
                     PAVLIRTFREFRVTMLLIVPAGLKLLDSAIQRKVDASGRHDTFERLHRIARRLPRFAK
                     RLLFRPVIGQFGGRFRTLAIGAAALEEDLATRWTDMGFDVLQGYGATELSPAVAFTRP
                     SRNRSGTVGEAVPGVEIRIAEDGEIQVRGPNVFAGYWEDRDATRAVLDGEGFYHTGDI
                     GQLDGEGFLTLRGRKKDMLAMPDGTKVYPEDIEAVLGRDTRLRDATVVGWQPPRGELR
                     VQAVLLLDDPALADAVVRDANAQLGAHQQIRGVTIWPDEDLPRTHTLKVKKPIVLARL
                     AELESGATTTHSATGPAAPIAVSSLEGLVASVANVPIASVQPESRLSTDLNMDSLARI
                     ELLSVIEEEMGTFVDDGDLDPDTTVAQLSSLVDAAKEQKHETGIFGWPLSPIARAAGI
                     LIQILVLVPVFFSMYRVRVTGLERLKGLKGPALFTPNHCLHNDVGIILTRIPLRWRWH
                     LSVAAAADDIFGNPVRGFGSALIGNAFPLAREGAVRRSLELLGARLDRGFSILIFPEG
                     KLTVGGPTQPFKSGTGLVAVEGATPVVPMRLKVTRMSRLDARREPALAGERLPWRGEV
                     ELVFGDPIHFAWDTDHLAATQQLEAAVAAL"
     gene            complement(6329..7366)
                     /locus_tag="VIN32_07355"
     CDS             complement(6329..7366)
                     /locus_tag="VIN32_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017239380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M36 family metallopeptidase"
                     /protein_id="HEY8922428.1"
                     /translation="MSRSRRSRVLTSLVAVAILALAPANASAAPFNGGGGTASALVFF
                     PNPVVTSGNLALTDEKDADYPALNNERVTVLLTNLDGSGNLCGAWACVTNSTKSGAFE
                     TDGTYYYTRHDERFEQVMAYYWITQSQLYLQSLGFGAGGPYRGINDNPQSVRIDQWGV
                     DNSFASDAPKDQMVFGKGGVDDAEDAEVIVHEYGHQIHFSQSPTFYSSVEARSIGEGF
                     SDYWAATVTEVIAGVQPDPECIAEWDSVSYTAGPVHCLRRLDANLTYPADLNGRVHHD
                     GTIWSQALWQIRASLGNVRADTLILMAQFDWTGTTMPDLANRTVAAAQSLYGNGAAVK
                     VRQAFEARGIL"
     gene            7535..>7719
                     /locus_tag="VIN32_07360"
     CDS             7535..>7719
                     /locus_tag="VIN32_07360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEY8922429.1"
                     /translation="MARDEKLDLLHSIPLFDRFDRKHLERLGMLTEEVDVPAGKVLIR
                     QGELGDDLMVIVTGTVDV"
BASE COUNT         1209 a         2666 c         2625 g         1219 t
ORIGIN      
        1 ccgacccgtc atgtcgcgct agccgctgat cacgacactg atagctgtga ctgaggccgc
       61 ggcaacgacc ggagcgaacg tgaccacgat cgtggtaccg gcgccggggg tcgtgcccgt
      121 ggcctcaccc aacgacgcgc cggcggcggt cgcgagggtg accttgtacg gcttggtgta
      181 gcacgtggcc gccaggccgg tgatcgttac ggtgccgacc gtatagcccg ggccgggagc
      241 ggccgcggtg taggttgatg cgtaggtggc attcatcgcg ccagcctggc aggcagcgac
      301 gaccgtggtc gccgagccga ggctgtcgct ggtgaggttg aggctcgcag ccataccgaa
      361 aacgagccca aaggtcatga gacccgcgag gacggcgccc acaatgcgtt tcatcaaaca
      421 agctccgaat atgtacatcg cccccgacca cggcatttga tcgctggaac aagttccgca
      481 acattcccca aaaggaccag cgcgggggag tactagcgga tggggtccgg ggcgatcgct
      541 acgtcggatt gagctccgag cgcagcgcgt gcggggtctg ctgctctgac agcagccggc
      601 tgaaaccgtg gctctgacca tgcacctcga gccactcgga gacgaggcgc cgaccctcga
      661 cctgggcaag cggctgcgtg cgcaggaggg gatcctgcag ccgccagatc acctcgtcga
      721 ggctctcgtc gcccaccagc ccctcctccg caaggtagga gcggacatct tcgacaggct
      781 cgccgtcgcg gtggagggcc accgccgcgt tcccaagggc cgggctcaat agacgccgcg
      841 cgcgctgcac catgagctcc gcctcgacat ccaggcgcct gtcgaccgag cgtgccagcc
      901 gctggagctc cagccccagc tcctggtcgc tcatcacgac ctcccggccg atccccgcca
      961 tcccctcgct gaccaccgcc tgcggggaga gcgtcgtcgt cagcgtgagc tcttcgtgcc
     1021 cggctgacgc cagcaggtcg tccttgaccg atgcctccgc gtggtggccc ggatagccct
     1081 cttgagcgac ggtctccgca agagttgcga aggtcagcgg ccggtcgaca ttcaagcgga
     1141 ccacggagat ccctgcgccg aggtagtgag cgtcgatcgc ccagtcgacc ccatgtgccg
     1201 cttcgatgcg gagcgattca ccttcgggca gccagagctg cgctcgcgtc ctcgtcctca
     1261 gtcgggccga gagctccgaa gcgagggcaa tggctcgctc gggagggacg cgactgcgcg
     1321 cgtcgtgctc cgcgagccgc tctcgcaggg tcgcaccccc agggagcgcg gagtccagca
     1381 tgcggcgcgc ggtatcgaag gtggcatcgg gctcgagctg cacttcgatg tcgtagaggt
     1441 cctcgacaag gtcgacgtag ccgatctcct ctccgccgac ccagcgtgcc agcgctccca
     1501 tcgccccgac ctgggcaatg agccaggtgg cgcggcgacg gcgcgcagcc gtatcggcct
     1561 ggagctcagc ggccattccc gccagctgca tggcttcgtc gtgcagctcg gcggccgggg
     1621 tgagtggctc gccccccacc gcctcagaga gctcggccgg gccgtggtac gaagcgacca
     1681 gcctgggaag gtgccgttca aggcgaagcc ccagcagcag gtagcgcagc gcgatctcgt
     1741 ccacgatcca gatgctagcg tcgttgacag ggttcgctgc cgtgggtacc ttcgcgaggc
     1801 acgcaagcaa gcacgcagac ggaaaggaga cgcccgatga ccaccgttgc cgtcgcgcgc
     1861 cctcgtctgc atgggatcgc ctcgcggccc tgatcatgcg ttgggcgcgc tccgccgccc
     1921 acctgcgacc cgatcgagtg gagaacaacc cgtgcctctg acgtccgtgc ctacgcctgt
     1981 ccgtgctgcg gctgaagccg ccctgaccgg aatcgtgctg cgcgcccgca gcggcttcta
     2041 caccgtccgc ctcgatgacg gaaccctgct ggaatgccgc ctccgcgggc gggtcaagca
     2101 ggaccgaggg aactccgacc tggtcgtcat cggcgaccgc gtggccgtcg cgccgctcga
     2161 ggatggagac gggatgatcg agtccgtgga gccgcgccga acgcgcttct cgcgccgcca
     2221 gccgggtcct cgcggcagct ggaaggagga catgatcgtc gccaacctcg accaggtgtt
     2281 ggtcgtgttc gcctgcgccg acccgatgcc acacctgcgc atggtcgacc gcttcctggt
     2341 ggtggccgag cacaaccagg tcgaggcggt gctcgtggcc aacaaggtcg acctggtcgg
     2401 gctgcccgcg gctcggagcc tgttcgccgg gtaccaggag atcggctatc cggtgcacta
     2461 cgtctcggcg cgcgagggga tcgggctcga ggagctggcc gatcggctgg tcggtcgcgt
     2521 cagcgtcgtc acagggccat ccggagtcgg caagtcgacg ctcctcaacg ccatccagcc
     2581 cgggctccgg atcgagaccg gcgcggtcag cgagacggtg cacaagggac gccacaccac
     2641 gacctcggcc gagctgcatc cgctgaccgc ggccggtgga gggtacgtgg ccgacacgcc
     2701 cggcctccgg gagctcggcc tctggcaggt tccccccgag gagctggcgt ggtgcttccc
     2761 ggagatgcgc gactacatcg gcaagtgcgg cttcaacgat tgccgccaca tcagcgagcc
     2821 gcgatgcgca gtgctcgcag ccgtggcggc cgggcggatc agcacggccc gtcacgactc
     2881 ctaccgccgc ctcctcctga acgaggaata ggccgaggcg gccctatgat ggccgcccat
     2941 ggagatcgcc cccggcgtcc acgccgtcag gctgctgagc gtctacgcct ttctcatcga
     3001 cgagccgcag ctgacgctga tcgacgctgg cctgatcggt tcggccggga gggtccagcg
     3061 ccatgtcgag cggatcgggc gctccctgga cgacctggtc cgcatcatct gcacgcacgc
     3121 tcacccggac cacatcggcg gcgttcgaga gctggccggc gagcgggatg tcgaggtgct
     3181 gatgcatacg gccgatctgg atgggttgag cgtcgggctg cgcgacgccg tcgcgaaccg
     3241 caatcgcggg cagctgatcg cctacttcac gcggcatccc ggcgaggcga cgccgatcga
     3301 ggatggccag gtgctgccga tcctcggagg gctgcgggtg gtccacaccc caggccacac
     3361 gccgggcagc atctgcctgt acgccgagcg ccacaagctc ctcttcgtcg gcgacaccct
     3421 ccaggtgatc cggggcaagg tcacctatgc cagcagggtg ttcagcgagg acctcgcgct
     3481 ggcccgcgcc agcgtggctc gcatggccga gctggacgtc gagacgatcg ccttcagcca
     3541 ctacccgccg ttgcgtgagg gggcgaacga ggtgctgcgc gggctcgcag agcgggccgc
     3601 cgccatgtcg gaccgtaagc ggagggccgc aagcctctag ccactgccgg acgccggtcg
     3661 taaggtaggc ggtgagggag aggttccatc gccacactca ctccgatcgc acccgccgcg
     3721 cgcgaaccga gcccgccgac agcggtcgtc gccgaccgcg cgaggcccgg tggagcgacc
     3781 cttatcgacc tgctgcgcga atcggcgcgc cgcttcgcgg atcgcccggc actgctgatc
     3841 aagcccggct ttcgtactcg catatggacc tatggggacc ttgccgacct cgccccgaag
     3901 gtcgccagcg tgctcgcaga tcaggggctg gagcgcggtg atcgggtcct gatctgggga
     3961 gtcaaccggc ccgagtggtc gctcggcttc ttcggggcgc tcttcgcggg cctcgtgctg
     4021 gtgccgctcg acgtccgcag ccagccggaa ttcgtcgcca aggttgcgca gcgcacccgc
     4081 gccaggctgg tgctggcctc gacccaaacg gcgtcgctgg cagaggggct gaacctgcca
     4141 ctgctcctga tcgagtcgct ccccgaccgc gcccgcaacg tgacgccgct gcccacgccc
     4201 gacatcgttg cgagcgacgt ggcggaggtg gtcttcacct cgggcaccac cggcgagccg
     4261 aagggggcga tgctgacgca cggcaacctg gtcgccaacg ccaccgcact gcaggacgtc
     4321 ttccccttca agccggacga gcgactcctt tcgatcttgc ccctctcgca catgttcgag
     4381 cagacctgcg ggctgatcgc gccgttcctt gtcggggcct cgatcgtcta tcccgtcagt
     4441 cgtcagccgg cggtgctgat ccggaccttc cgggagttcc gcgtgaccat gctgctgatc
     4501 gtgcccgccg gcctgaagct gctggactcg gcgatccagc gcaaggtgga cgcctccggg
     4561 aggcatgaca ccttcgagcg acttcaccgc atcgcgcgtc gcctgccgcg cttcgcgaaa
     4621 cggctcctct tccgtccggt catcgggcaa ttcggcgggc gcttccggac cctggccatc
     4681 ggggcggcag cgctcgaaga ggacctcgcc acgcgctgga ccgatatggg attcgacgtc
     4741 ctgcagggct acggcgctac cgagctgtcc cccgccgtcg ccttcacgcg gccctcccgc
     4801 aaccgaagcg gaacggtcgg cgaggcagtg cccggagtcg agatccgtat cgcggaagac
     4861 ggcgagatcc aggtccgcgg cccgaacgtc ttcgctggct actgggagga tcgcgatgcg
     4921 accagggcgg tgctcgatgg cgagggcttc taccacactg gggacatcgg ccagctcgac
     4981 ggcgagggat tcctgacgct gcgcggccgg aagaaggaca tgctggcgat gcccgacggg
     5041 accaaggtct accccgagga catcgaggcc gtgctggggc gcgacacgcg cctgcgcgat
     5101 gcgacggtcg tcggctggca gccgccgcga ggcgagcttc gggtccaggc cgtgctcctt
     5161 ctcgatgacc cagccctggc ggatgccgtg gtacgcgacg ccaatgcgca gctcggcgct
     5221 catcagcaga tccgcggagt gacgatctgg ccagatgagg acctcccccg aacgcacacc
     5281 ctcaaggtca agaagccgat cgtgctcgct cgcctggcgg agctcgagag cggtgcaacg
     5341 acgacccaca gcgcaaccgg gccggctgcg ccgatcgccg tctcaagcct cgagggactg
     5401 gtggcatcgg tggcgaacgt gccgatcgca tcggtccagc cggagtcccg cctgtcgacc
     5461 gacctgaaca tggactcgct ggcgcggatc gagctgctga gtgtcatcga ggaggagatg
     5521 gggaccttcg tcgacgacgg cgacctcgac ccggacacga cggtggcgca gctctcctcg
     5581 ctggtcgacg cggcgaagga gcagaagcat gagaccggca tcttcggctg gcccctcagc
     5641 ccgatcgcgc gagccgcagg gatcctgatc cagatcctgg tcctggtgcc ggtcttcttc
     5701 agcatgtacc gcgtccgcgt gacgggcctc gagcgactca agggcctgaa gggccctgcc
     5761 ctcttcaccc cgaaccactg cctgcacaac gacgtgggga tcatcctcac gcgcattccg
     5821 ctccgctggc gctggcacct gtcggtcgct gccgcggccg acgacatctt cggcaacccg
     5881 gttcgtggct tcgggtcggc actcatcggc aacgcgttcc cactggcccg ggagggggcg
     5941 gtgagacgct ccctcgagct gctcggcgcc aggctcgacc gcggcttctc gatcctgatc
     6001 ttcccggagg ggaagctgac cgtcggcggc ccgacccagc ccttcaagtc ggggaccggc
     6061 ctcgtcgccg tcgaaggagc caccccggtg gtgccgatga ggctgaaggt gacccgcatg
     6121 tcgcgcctcg acgcccgccg cgagcccgcg ctcgccggtg agcgcctccc gtggcgggga
     6181 gaggtggagc tggtcttcgg cgatccgatc cacttcgcgt gggacactga ccacctggct
     6241 gcaacgcagc agctggaggc cgctgttgcc gcgctctgac gcgccccacg cgctgccccg
     6301 acctctgctc ttcctgcgct gaggtccgct acaggattcc gcgtgcctcg aacgcctggc
     6361 gcaccttgac cgccgcaccg ttgccataga gggactgcgc cgccgcgacc gtgcggttcg
     6421 ccagatccgg catggtcgtg ccggtccagt cgaactgcgc catcaggatc agggtgtcgg
     6481 ccctgacgtt tccgagcgag gcgcggatct gccacagggc ctgcgaccag atcgtgccgt
     6541 cgtgatggac tcgcccgttg aggtcggcag ggtaggtcag gttggcgtcc aggcggcgca
     6601 ggcaatgcac cgggcctgcc gtgtaactga cggagtccca ttcggcgatg cactccgggt
     6661 cgggctgcac gccggcgatc acttcggtga ccgtcgcagc ccagtagtcg ctgaagccct
     6721 cgccgatcga gcgcgcctca accgagctgt agaaggtcgg tgactggctg aagtggatct
     6781 ggtgcccgta ctcgtgcacg ataacctccg cgtcctcggc atcgtcgacg ccacccttgc
     6841 cgaagaccat ctggtccttg ggcgcgtcgg atgcaaagga gttgtcgacg ccccactggt
     6901 cgatgcggac cgactgcggg ttgtcgttga tgccgcgata ggggccgccg gcgccaaagc
     6961 cgagcgactg gaggtagagc tgggactggg tgatccagta gtaggccatc acctgctcaa
     7021 agcgctcgtc gtgtcgcgtg tagtagtagg tgccatccgt ctcgaaggcg cccgacttcg
     7081 tcgagttcgt gacgcacgcc caggcgccgc acaggttgcc cgagccgtcc agattggtga
     7141 gcagcacggt cacccgctcg ttgttcagcg ccgggtagtc ggcatccttc tcatcggtca
     7201 gtgccaggtt gccgctggtc accacagggt tgggaaagaa gaccagcgcc gatgcggtgc
     7261 ccccgccgcc gttgaacggc gcggccgagg cattcgcagg cgccaaggcc aggatggcca
     7321 ctgcgacgag gctggtcagc acgcgtgatc gtcgggatcg ggacatcctg gctcctccgg
     7381 gtcgtgacga tggtcgtctc aaggtacgcg ggcccgcgcg agaccgtcaa accggctcat
     7441 gccgatagtg gtgcccgacg gcaccgcgtt tgggggtctt cgcaatcagg gcgcgaggcc
     7501 gtatgctccg cccctagcga cggaggaggc gccagtggca cgcgacgaga agctcgacct
     7561 gctgcacagc atcccgctct tcgaccgttt cgatcggaag cacctcgagc gactcggcat
     7621 gctgaccgag gaggttgacg tgccggccgg caaggtcctg atccgccagg gcgagctagg
     7681 cgacgacctg atggtgatcg tcaccggcac cgtcgacgt
//
LOCUS       DATCQR010000220        10954 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_213740, whole genome shotgun sequence.
ACCESSION   DATCQR010000220 DATCQR010000000
VERSION     DATCQR010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 10954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10954
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213740"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..518)
                     /locus_tag="VIN32_07365"
     CDS             complement(<1..518)
                     /locus_tag="VIN32_07365"
                     /inference="COORDINATES: protein motif:HMM:NF012964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEY8922430.1"
                     /translation="MTYGSPDDLDDMPRYLTAVRGGRPPSPEVIAEFRRRYEAIGGSP
                     LIPITQAQAAAVEERLRADGLDALAVAGMRFSEPSIAAGLAALAEAGCGEVVGIIMSP
                     QFSGVLMSGYQIALDEAGRALGAMAPEVRLAPSWYRNEHFTEAVASRIAEGLDRLPAA
                     ERERAPVLLTAHS"
     gene            complement(551..1603)
                     /gene="hemE"
                     /locus_tag="VIN32_07370"
     CDS             complement(551..1603)
                     /gene="hemE"
                     /locus_tag="VIN32_07370"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012876510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HEY8922431.1"
                     /translation="MLDAPTRPMTGEDRFLAALRHEQPDATPVWFMRQAGRALADYRA
                     LRERYPILTLAKTPELCAEVTLMPVLTFGVDAAVLFADIMVPLEPMGVSLEIQPDVGP
                     IIHHPLRSRADVDRLRLIEPAEDVAFVMDAIRLVRGELAGKQAVIGFSGSPFTLACYL
                     IEGRPSRDYAIAKAFMYGEPAAWHALMEKLAEVVIRYLNAQVAAGAQVVQLFDSWVGA
                     LAPGDYVTYVQPHVARIFAAVDGAPTIHFGTGTAALLELMADAGGDVIGIDARQSLDV
                     AWDRIGFQRGIQGNLDATRLLAGWDATREGALDVLTRAANRPGHIFNLGHGVLPETDP
                     ALLTRLVELVHAESAR"
     gene            complement(1596..2852)
                     /gene="hemL"
                     /locus_tag="VIN32_07375"
     CDS             complement(1596..2852)
                     /gene="hemL"
                     /locus_tag="VIN32_07375"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HEY8922432.1"
                     /translation="MTAVTATTRSGELMARAERLFPGGVNSPVRAFRAVGGVPRVIER
                     ASGARLWDVDGNELIDYIGSWGPMILGHAHPTVTAAVTEAAARGTSFGAPNSGEVVLA
                     EEIVAAMPSIEQLRFVNSGTEAAMSAVRVARAFTGRELIIKMAGGYHGHADALLVRAG
                     SGATGLPASAGVTAGAAADTRVIDYNDLDAAAEMLGHGNVAAVIVEPVAANMGVVLPE
                     PGYLEGLRRLTAQHDTLLIFDEVITGFRLARGGAQQFYGVVPDLTVLGKIIGGGLPVG
                     AYGGSANTMGLVAPLGPVYQAGTLSGNPLAMAAGLATLQALTPSVYAQLEKAGALLEA
                     GLAEAAAAASVPARVVRAGSLLTVFFGDDARFARFFHAMLDAGVMLPPSQHEAWFISA
                     AHGQAELDATLEAARRVFATLGDRDA"
     gene            complement(2849..3535)
                     /locus_tag="VIN32_07380"
     CDS             complement(2849..3535)
                     /locus_tag="VIN32_07380"
                     /inference="COORDINATES: protein motif:HMM:NF018484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="HEY8922433.1"
                     /translation="MSDERFVHAWLLRLDPAWRRRPAAEREAEIAAFCAAAERSAARL
                     VQHAYSTIGLRADGDLLLWRMAERIEEIEETAADLLTSGIGRWLTPAISMIGLTRPSQ
                     YVKRPSAQEQSLFSGERSPYLVVYPFVKSVEWYLTPAEERQEVMKGHMRVGHRFPQVR
                     QLLAYSFGLDDQEFIVAYETDDLVAFQDLVRELRETESRRSTVRDTPIITGIYRPLGE
                     ILRHLSGAAA"
     gene            complement(3532..4557)
                     /gene="hemB"
                     /locus_tag="VIN32_07385"
     CDS             complement(3532..4557)
                     /gene="hemB"
                     /locus_tag="VIN32_07385"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEY8922434.1"
                     /translation="MSLTFETGTELKIGLRGRRLRRTPALRGLVRETRLHPQMLVQPL
                     FIQAGAQGREPIGAMPGQDRLGLDALPEEAARLRAAGVRTVLLFGLPGSKDVIGSGAW
                     AADGIVQEAIRVLRTADHELVIIADVCLCEYTSHGHCGVLARSGDDATVDNDASIELL
                     ARTAVSLAEAGADIVAPSAMMDGQVAAVRSALDEAGHGQTAILAYAAKHASAFYGPFR
                     EAADSAPAFGDRRSYQMDPANGREARREIDADLAEGADMVMIKPAMPSLDLVAAARAR
                     TDVPIAAYQVSGEYAAICAAAANGWLDRRAVALESLVAIARAGADIIVTYFAAEAASW
                     LRDEATR"
     gene            complement(4554..5309)
                     /locus_tag="VIN32_07390"
     CDS             complement(4554..5309)
                     /locus_tag="VIN32_07390"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEY8922435.1"
                     /translation="MTTLLVTRPAGQAAELVALLAERGIDAISVPTVGIAGPSVGGPL
                     DDAIRSLDGAAWLVITSVNGAASLLERMAALGRSLPAGVRVAAVGPATAAALESGGVR
                     VDHVPSRYRTVAIAEGLGDVARRRVVLARADAATRDLRDALLERGALVEEVVAYRTVE
                     GPPESRDRVRAVLMQPLDGVTFTSGSTVRGLHALLSAPEALRATALPAYCIGPVTARV
                     ARRVGFSVPVVAARHTVAALADAIHAHISKEIA"
     gene            complement(5306..6202)
                     /gene="hemC"
                     /locus_tag="VIN32_07395"
     CDS             complement(5306..6202)
                     /gene="hemC"
                     /locus_tag="VIN32_07395"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEY8922436.1"
                     /translation="MSTLASTLRLGTRGSRLAMAQAHEAASALTEMLGLDSVEMVEIH
                     TKGDAISQRRPAGLLMVGDGQFTGELEQALLEDRIDIAVHSLKDLPTTSNPALRLGAI
                     LERGDPRDCLLSGHAGGLDGLPFGARIGTSSVRRAAQLAAARVDLVARPIRGNVDTRL
                     RRLAAGEYDALLLAAAGLDRLGVPFSEDDRLPLELMLPAPGQGALALQVRADDVPLIA
                     PLDHEPTRVAVTAERALLRALGGGCLAPLGALAEVMDGRIRLRAAYEGRNGFTRVDLS
                     GPADRSGELIAAAATQIRQATA"
     gene            complement(6199..7413)
                     /locus_tag="VIN32_07400"
     CDS             complement(6199..7413)
                     /locus_tag="VIN32_07400"
                     /inference="COORDINATES: protein motif:HMM:NF013641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8922437.1"
                     /translation="MPPALQDMGAAPADRGTQLDRLLVASATVRDFDTDARETLAAAL
                     ETGSGDAGERVVLHTCHRVELIAFPGATDDLADIPEAMQRTSGLAAAQRVMLVAGGLD
                     STVLAEEQVLGQVRDAYQSALNRGHTGAVTNELLRRAIRFGKRVQSFAQPTGDRSLAD
                     RSARWVEERLGPRPSGRPLRALVIGTGEMGRALAGHFSALDAWVTVASRSGDRAEDSI
                     AALPHGERHRAALISQVLAGPLAYDVVAIAVRGGTHRLDSRHVVGPRPLVVDLSSPSA
                     VTVDAAALLGDRLLDLDRLGVASQARRLTGAVERRLRDEATVEANAFAAWLEIRASGD
                     AIAMLRAHGDDVRRRHLERLRLKSSLDKEQAAAVEAMTVAMLGELLHGPTLRLRHDPD
                     AAARVREVFGIE"
     gene            complement(7599..9236)
                     /locus_tag="VIN32_07405"
     CDS             complement(7599..9236)
                     /locus_tag="VIN32_07405"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013133556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEY8922438.1"
                     /translation="MSPRDPATTVYANGRILTCDPGQPSAAAVAVRDGRIMAVGEAAV
                     VEPHLDAGATTIDLGGRTMVPGFIDAHNHMACTAETFFAVDASPRSVSSIAELVAAVA
                     EAAARTPEGAWIRGFGMDWTRFAEGRRPTRWDLDEAGSEHPLVILHVSGHYALVNSRA
                     LADAGLSETAADPEGGSLERDESGRLTGLLLDSATNLVLQSSVDICGHGPNIHTAVET
                     DELARMIGEASRRYLAAGLTTICDPQVTSREMTAYQHARAQGDLPIRTVMLPLSHQLD
                     EYLAIGLVGPMGDDHLRIGAMKFYTDGAITGGTAMFSQPMGSRGQYPGSLYHQPGELA
                     DLLRRAAGAGWQLAIHTMGDRAMGIMLDAVEAAMNAAPDGDIRHRIEHSTWPTPEQLA
                     RIARLGMVPVTQPGSIAELGDIWHDHLGERVHRAMPLRDQLELGIRPAISSDAFVQSY
                     RPLDTIAAAMHRVTPSGFQVGPDQELTIEEALAAHTINAARALHMEDRIGSIEVGKLA
                     DFAVLDGDLLATPAEAIRQIPIWMTVISGEVLHSTAV"
     gene            9302..9409
                     /gene="glnQ"
                     /locus_tag="VIN32_07410"
                     /pseudo
     CDS             9302..9409
                     /gene="glnQ"
                     /locus_tag="VIN32_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727773.1"
                     /note="similar to ATP-binding component of ABC
                     transporters; incomplete; partial in the middle of a
                     contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
     gene            complement(9416..10177)
                     /locus_tag="VIN32_07415"
     CDS             complement(9416..10177)
                     /locus_tag="VIN32_07415"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY8922439.1"
                     /translation="MLRDEVPAPLRNDFEHSSLPIHEVIQRSLRRRIEDGELTEGRQL
                     PSEAALQREFQASRTPVRQALRRLQTDGLIIRAQGKGSFVASAKIGVALRQMESFGSE
                     LRRRGHAVEARTLGVEWAAAPTEVARSLGLKDDHATHLRRLLVVDQIPLTFFEHWIAP
                     TIAHSVFVAAGDFPSLYELLTEHGREPWYGTETISASVATEEMARLLDYPEGGPLVTM
                     VRLTRDFERLPIEYTTYVVRADRYAYEINLMRHRR"
     gene            10270..10950
                     /locus_tag="VIN32_07420"
     CDS             10270..10950
                     /locus_tag="VIN32_07420"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEY8922440.1"
                     /translation="MTTAHVPEPRGVVLDLDGTLVDTVGTRIDAWLRTFAEEGITPGR
                     SIVAELIGSDGRQLARIVFDAAGIELDDERAERIDARSGAIYSELNVDPQPLPSVTGF
                     LDALDKLKIPWAIATSSRREQVGRSVAALHRHEPPLIADGTAVRLAKPHPDLLLAAAG
                     ALEVHPAECWAVGDSTWDMVAAEAAGMVAIGITAGSAMSPDQLRAAGAAVVFPTMARL
                     IPHLSSGT"
BASE COUNT         1684 a         3886 c         3765 g         1619 t
ORIGIN      
        1 gagtgggcgg tcagcagcac cggtgcgcgt tcccgctcgg ccgccggtag ccggtcgagc
       61 ccctcggcaa tccggctggc taccgcctca gtgaagtgct cgtttcgata ccacgacggc
      121 gccaggcgga cctcgggcgc catcgctccg agcgcacgcc ccgcctcgtc cagcgcgatc
      181 tggtagccgc tcatcagcac cccggagaac tgcgggctca tgatgatccc gaccacctcg
      241 ccgcagcccg cctccgccag ggcagccagg ccggccgcga tgcttggctc cgagaagcgc
      301 atcccggcaa cggcgagcgc gtccagccca tcggcccgca gccgctcctc gacggcggcc
      361 gcctgtgcct gggtgatggg gatcagcggc gagccgccga tcgcctcata ccgtcggcgg
      421 aactccgcga tcacctccgg cgacggcggt cgccctccac gcaccgcagt caggtagcgg
      481 ggcatgtcgt cgaggtcgtc cggcgagccg tacgtcatca gcagaacgcc cagccggcgc
      541 ggctccgacg tcatcgggca ctctctgcat ggaccagctc gaccaggcgg gtcagcaggg
      601 ctggatccgt ttcgggcagg acgccgtggc ccaggttgaa gatgtgcccc ggccggttgg
      661 cagcccgagt cagcacgtcc agcgccccct cacgcgtggc atcccagccg gccagcagcc
      721 gggtggcgtc caggttgccc tggatgcctc gttggaagcc gatccggtcc caggcgacgt
      781 cgagcgactg gcgtgcgtca atcccgatca cgtcgccgcc ggcgtcggcc atcagctcca
      841 gcagcgccgc ggtcccggtc ccgaagtgga tcgtgggagc gccgtccacg gccgcgaata
      901 tgcgcgccac gtgcggctgg acatacgtca catagtcgcc aggcgcgagg gcaccaaccc
      961 aggagtcgaa gagctgcacc acctgtgccc cggctgcgac ctgtgcgttg aggtagcgga
     1021 tgaccacctc ggcgagcttc tccatcaggg catgccaggc cgccggctcg ccgtacatga
     1081 aggccttggc gatcgcgtag tcgcgcgacg gccgcccctc gatcaggtag caggccagcg
     1141 tgaacggcga ccctgagaag ccgatgaccg cctgctttcc ggccagctcg ccgcgtacga
     1201 ggcggatcgc gtccatgacg aacgcgacgt cctcggccgg ctcgatcagc cgcaggcggt
     1261 ccacgtcggc gcgggagcgg agcgggtggt ggatgatcgg cccgacgtcg ggctggatct
     1321 cgagcgagac gcccatcggc tcaagcggaa ccatgatgtc cgcgaagagg accgccgcgt
     1381 cgacaccgaa ggtgaggacc ggcatcaggg tgacctcggc gcacagctcc ggggtcttgg
     1441 ccagcgtcag gatcgggtag cgctcccgca gagcgcggta atctgccagg gcgcgtcccg
     1501 cctggcgcat gaaccagacc ggcgtggcgt cgggctgctc gtggcgcagg gcggccagga
     1561 accggtcctc gccggtcatc ggtcgcgtgg gggcatcaag catcccgatc tccaagcgtg
     1621 gcgaagacgc ggcgtgccgc ttccagagtc gcatcgagct cagcttgccc gtgtgcggcg
     1681 gagatgaacc aggcctcgtg ctgggagggc ggcagcatca cgccggcatc gagcatggcg
     1741 tggaagaagc gggcgaaccg cgcatcgtca ccgaagaaga ccgtcagcag cgagcccgcc
     1801 cggacgacgc gcgcaggcac gctggccgcc gccgccgctt cggcgaggcc tgcctccaga
     1861 agggcgcctg ccttctccag ctgcgcgtag accgatggcg tgagcgcctg cagcgtcgcc
     1921 agcccggctg ccatggccag cggattgccg gacagggtgc cggcctggta gaccggcccg
     1981 agtggggcga ccagccccat cgtattggcc gatccaccgt aggcgcccac cggcaggcca
     2041 ccgccgatga tcttgcccag caccgtcagg tcggggacga ctccatagaa ctgctgggca
     2101 ccgccccgcg ccaggcggaa gcccgtgatc acctcgtcga agatcagcag ggtgtcgtgc
     2161 tgagcggtca ggcggcgaag cccctcgagg tagcccggtt cgggcagaac gacgcccatg
     2221 ttggccgcga ccggctcgac gatgaccgcc gccacgttgc cgtgtccgag catttcggcc
     2281 gccgcgtcga ggtcgttgta gtcgatcacc cgggtgtccg cggcggcgcc ggcggtcacg
     2341 cccgcgctgg cgggcaaccc cgttgcaccg gatccggcgc gcaccagcag ggcatcggcg
     2401 tgcccgtggt agccgccggc catcttgatg atcagctctc gaccggtgaa cgcccgcgcc
     2461 acccgcacgg cggacatggc ggcctccgtc ccgctgttga cgaagcgcag ctgttcgatc
     2521 gacggcatgg ccgccacgat ctcctcggcc agcacaacct cgccggagtt cggcgctccg
     2581 aacgacgtgc cccgcgcagc cgcctccgtg acggcggcgg tcaccgtcgg gtgggcgtgg
     2641 cccaggatca tcggtcccca tgagccgatg tagtcgatca gctcgttgcc gtccacgtcc
     2701 cacaggcgcg cgcctgatgc tcgctcgatc acgcgcggga cgccgcccac cgcgcggaag
     2761 gcgcgcaccg gggagttcac gccaccgggg aatagccgct ccgcgcgggc catcagctcg
     2821 ccggaccggg tggtggccgt cacggcagtc atgcggcggc tcccgacagg tgccgcagga
     2881 tctcgcccag cgggcggtag atgccggtga tgatcggcgt gtcgcgcacc gttgagcggc
     2941 ggctctccgt ctcgcgcagc tcgcggacca ggtcctggaa ggcgacgagg tcatcggtct
     3001 cgtaggcgac gatgaactcc tggtcgtcga ggccgaacga gtaggcgagg agctggcgca
     3061 cctgcgggaa ccgatgtccg acgcgcatgt ggcccttcat cacctcctgc cgctcctctg
     3121 ccggggtcag gtaccactcc acgctcttca cgaacgggta gaccaccagg tacggggacc
     3181 gctcgccgga gaagaggctc tgctcctggg cgctcggccg cttgacgtac tggctcggcc
     3241 gcgtcaggcc gatcatggag atggcgggtg tcagccagcg accgatgccg ctggtcagca
     3301 ggtcggccgc cgtctcctcg atctcctcga tccgctcggc catcctccac agcagcaggt
     3361 ctccatccgc ccgcaggccg atggtcgagt aggcgtgctg caccagccga gccgcggagc
     3421 gttcggcggc ggcgcagaag gctgcgatct ccgcctccct ctcagcagcc ggccgtcgtc
     3481 gccacgccgg atcgaggcgc agcagccagg cgtggacgaa gcgctcatcg ctcatcgcgt
     3541 cgcttcgtcg cgcagccagc tggcggcctc ggcggcgaag taggtgacga tgatgtcagc
     3601 gccggcccgc gcgatcgcca ccaggctctc gagcgcgaca gctcgacgat caagccatcc
     3661 gttggccgcg gcggcgcaga tggccgcgta ctccccgctc acctggtagg cggcgatggg
     3721 cacgtcagta cgagcgcggg cggcggccac caggtcgagc gacggcatcg ccggcttgat
     3781 catgaccatg tccgccccct cggccaggtc ggcatcgatc tcgcgtcgcg cctcccgccc
     3841 gttggccgga tccatctggt agctgcgccg gtcgccaaat gccggtgcgg agtcggcggc
     3901 ttcgcggaac gggccgtaga aggcggaggc atgcttggcc gcatacgcca ggatcgccgt
     3961 ctggccgtgc ccagcctcgt ccagcgcgct gcgaacagcg gccacctggc cgtccatcat
     4021 ggcgctgggc gcgacaatgt cggccccggc ctccgccaac gagacggccg tgcgggccag
     4081 cagctcgatc gaggcgtcgt tgtcgacggt ggcatcgtcc ccgctcctgg ccaggacgcc
     4141 gcaatgaccg tggctggtgt actcgcacag gcagacgtcg gcgatgatga ccagctcgtg
     4201 gtcagcagtc cgcagcacgc ggatcgcctc ctggacgatc ccatccgcgg cccacgcgcc
     4261 cgagccgatg acgtccttgc tccccggcag gccaaagagg agcaccgttc tgacgccggc
     4321 cgcccgcagc cgggctgcct cttcgggcag tgcgtcgagg cccagccgat cctgtcccgg
     4381 catggccccg atcggctcgc gtccctgggc gccggcctgg atgaagagcg gctggacgag
     4441 catctgggga tgcaggcggg tctcgcggac caggccacgg agtgccggcg tccggcgcag
     4501 ccgtcggccg cgcaagccaa tcttcaactc ggtaccggtt tcgaaggtga ggctcatgcg
     4561 atctccttgc tgatgtgggc atggatcgcg tccgccagcg cggcgaccgt gtgccgggca
     4621 gcgaccaccg ggaccgagaa gcccacccgg cgcgcaaccc tggcagtgac cggaccgatg
     4681 cagtaggccg gcagcgcggt cgcccggagt gcctccggcg ccgagagcag cgcatgcagt
     4741 ccgcgcaccg tcgagccgct ggtgaaggtg accccatcca gcggctgcat cagtaccgcc
     4801 cggacccgat cccgcgattc cggcggccct tcgaccgtgc ggtaggcgac cacctcttcg
     4861 accagcgccc cgcgctcgag cagagcatcg cgtaggtcgc gggtcgccgc gtcggcgcga
     4921 gccagcacca cgcgcctgcg cgccacgtcg ccaagcccct ctgcaatcgc cacggttcgg
     4981 tagcgggacg gcacgtggtc gacacgcacg ccgccagact ccagggccgc tgcggtcgcc
     5041 ggcccgacgg ccgccacgcg gacacctgcc ggcagggagc ggcccagggc cgccatgcgt
     5101 tccaacaacg aggcagctcc attcacgctg gtgatgacca gccaggcggc accgtcgagg
     5161 gatcggatcg catcgtcgag cgggccaccc accgaagggc cagcgatccc cacagtcggg
     5221 acgctgatcg cgtcgatgcc ccgctccgcg agtaacgcga ccagctcggc cgcctggccg
     5281 gcaggccggg tgaccaggag cgtggtcacg ccgttgcctg cctgatctgt gtcgctgctg
     5341 ccgcgatgag ctcgccggag cgatcggccg ggccgctgag gtcgactcgc gtgaacccat
     5401 tgcgaccctc gtatgccgcg cgcaggcgga tccgcccgtc catcacctcc gcgagcgcac
     5461 caaggggagc caggcaccca ccgccgagcg cgcggagcag cgcacgctcg gcggtgaccg
     5521 caacccgggt cggttcatgg tcgagcggag cgatcaacgg cacatcatcg gctcggacct
     5581 ggagcgcaag cgccccctgg ccgggtgctg gaagcatgag ctccagcggg agccgatcgt
     5641 cctcgctgaa cggcactccc aggcggtcca gccccgcggc agcaagcagc agtgcgtcgt
     5701 actcgccggc cgcgagacgc cgcagccggg tatcgacgtt tccacggatg ggacgggcca
     5761 cgaggtcaac cctggccgct gccagctgcg ccgcgcggcg cacgctgctg gtcccgatcc
     5821 gcgccccgaa cggcaggcca tcgaggccgc cggcgtggcc gctcagcagg caatcccttg
     5881 gatcgccgcg ttccaggatc gcgcccaatc gcaaggccgg attcgacgtc gtggggaggt
     5941 ccttgagtga atgaaccgca atgtcgattc ggtcctcgag caacgcctgt tccagctccc
     6001 cggtgaactg cccgtctccc accatcagca gtccggctgg ccgtcgttgg ctgatcgcgt
     6061 cccccttggt gtggatctcg accatctcga ccgagtcaag gccgagcatt tcggtcagtg
     6121 cggatgcggc ctcgtgtgcc tgcgccatcg ccaggcggct gccacgagtg ccgagtcgca
     6181 gggtgctggc gagggtgctc attcgatccc gaacacttcc cgcacccgtg ccgcagcgtc
     6241 agggtcgtgg cgcagacgca gggtgggtcc gtggaggagc tcaccgagca tggcgacggt
     6301 catggcttcg acggctgccg cctgctcctt gtccaggctg ctcttcaggc gcagccgctc
     6361 aaggtgccgc cgcctgacgt cgtcgccgtg ggctcgcaac atggcgatgg catcgccgct
     6421 ggcgcggatc tccagccacg cggcgaatgc attggcctcg accgtggcct cgtcgcgcag
     6481 gcgacgctcg acagccccgg tgagccggcg tgcctgactg gcgacgccta accgatcgag
     6541 atccaggagt cggtcgccca gcagagcagc cgcgtccacc gtgacggcgc tgggcgagga
     6601 gagatcgacc accagcgggc gcggaccgac cacgtgccgg gaatcgaggc gatgcgtgcc
     6661 gccgcggacc gcgatcgcca cgacgtcgta tgccagcggc cccgccagga cctgcgagat
     6721 gagggctgcc ctgtggcgct cgccatgcgg cagagcggcg atggagtctt ccgcgcgatc
     6781 cccgcttcgc gacgcaaccg tgacccaggc gtcaagagcg ctgaagtgcc cggcgagggc
     6841 ccgtcccatc tcgccggtgc cgataacgag ggcacggagt gggcgccccg acggcctggg
     6901 ccccagtcgt tcctctaccc agcgcgcgct gcggtcagcc agcgagcggt cgccggtagg
     6961 ctgcgcgaag gactgcaccc gcttgccgaa gcggatggct cggcgcagga gctcattggt
     7021 gacagccccg gtatgtccac gattgagtgc ggactggtag gcgtcccgca cctgcccgag
     7081 cacttgctcc tctgcgagca cggtcgaatc gagtcctccc gcgacgagca tcacccgctg
     7141 cgctgcagcc agccccgacg tccgctgcat ggcctcaggg atatcagcca ggtcgtccgt
     7201 tgccccgggg aacgcgatca gctcgacccg atggcaggta tgaagcacca cccgttcgcc
     7261 ggcatccccc gaccccgtct cgagtgctgc cgcgagcgtc tcgcgggcgt ccgtgtcaaa
     7321 gtcgcgaacc gtggcgctgg cgacgagcag gcggtccagc tgcgtaccac ggtctgcggg
     7381 cgccgcgccc atgtcttgaa gagccggagg caagcgaaga tcctcgttgc caggggaagg
     7441 gagcgggcga acgctaggcg gcatcgtgct cggttgtcaa acagaaacat aggttttcct
     7501 atgtttccgc ccattcggcc cgcggctcag gggccatcca aagaagtact gctcgcccac
     7561 tgggctgcga tgggcctcac cggcgacttc tggaagctct agacggcggt cgagtggagg
     7621 acctccccgc tgatcaccgt catccagatc gggatctggc gaatcgcctc ggccggggtc
     7681 gcgagcaggt cgccatccag gaccgcgaag tcggccagct ttcccacctc gatcgagccg
     7741 atccgatcct ccatgtggag cgcccgggcc gcgttgatcg tgtgcgcggc aagggcctct
     7801 tcgatcgtca gctcctggtc cgggccaacc tggaagccgc tcggcgtgac ccgatgcatc
     7861 gcggccgcga tggtgtcgag cggccggtag ctctgcacga aagcatcgct gctgatcgcc
     7921 ggacggatgc ccagctcgag ctggtctcgc agcggcatgg cccgatggac gcgctcgcca
     7981 aggtggtcgt gccagatgtc accgagctcg gcgatcgagc cgggctgcgt gaccggcacc
     8041 atccccagcc gcgcgatgcg cgcgagctgc tcgggggtcg gccatgtcga atgctcgatg
     8101 cggtggcgga tgtcgccatc gggtgcggcg ttcatggccg cctccacggc gtcgagcatg
     8161 atccccatcg cgcggtcgcc catggtgtgg atggccagct gccagccggc cccggccgct
     8221 cgacgaagca ggtcggccag ctcgccgggc tggtggtaca gcgagccggg gtactggccg
     8281 cgcgagccca tcggctggct gaacatggcg gtcccgccgg tgatggcgcc atcggtgtag
     8341 aacttcatgg cgccgatgcg aaggtggtca tctcccatcg ggcccaccag gccgatggcc
     8401 aggtactcgt cgagctgatg cgacagcgga agcatcaccg tcctgatggg caggtcgccc
     8461 tgtgcgcgcg catgctggta tgccgtcatc tcacggctgg tgacctgcgg atcgcagatg
     8521 gtggtcaggc cggcagccag gtagcgccgc gatgcctcgc cgatcatccg cgccagctca
     8581 tcggtctcga cggcagtgtg gatgttgggg ccgtgcccgc agatgtcgac cgagctctgc
     8641 aggaccaggt tggtcgcgga atcgagcagc agcccggtga gccgtccgga ctcgtcccgc
     8701 tccagcgatc cgccctccgg gtccgcggct gtctcgctca ggccggcatc cgccagcgcc
     8761 cgggagttga ccagggcgta gtggcccgag acgtggagga tcacgagggg gtgctcactg
     8821 cccgcctcgt cgaggtccca gcgagtgggt cgccggccct cggcaaatcg ggtccaatcc
     8881 atcccgaagc cgcggatcca ggcaccttcc ggcgtccgcg ccgcggcctc ggcgaccgca
     8941 gctaccagct cggcgatgga gctgaccgat cgcgggctgg cgtcaaccgc gaagaaggtc
     9001 tccgcggtgc aggccatgtg gttgtgcgcg tcgatgaagc cggggaccat ggtgcgaccg
     9061 ccgaggtcaa tggtcgtcgc gccggcatcg agatgcggct ctacaacggc agcctccccg
     9121 accgccatga ttcgtccgtc ccgcacggca actgccgcag ccgagggctg cccggggtcg
     9181 caggtcagga tccgcccgtt ggcgtagacc gtcgtcgccg ggtcgcgagg gctcacgccg
     9241 gcatggtacg tctcttgtcc cgccaccccc agcgtggtac aaacccccga cggcgttcag
     9301 cgtgatgctg atggacgagg ggcgcatcat cgaggatgcc ccgcctgacg agttcttcgg
     9361 cagcccgcgc gaggagcgaa cccaaaggtt cctggaggcg gtccgctagg ttcgcctagc
     9421 gccgatgccg catgaggttg atctcgtacg cataccgatc ggcgcgcacg acgtaggtcg
     9481 tgtattcgat cggcaggcgt tcgaagtcgc gtgtcagccg gaccatggtg accagcgggc
     9541 cgccctcggg gtagtcgagg agcctcgcca tctcctcggt ggccaccgag gcactgatcg
     9601 tctccgtccc ataccagggc tcgcgtccat gctcggtgag aagctcgtag agcgagggga
     9661 agtcgccggc ggcgacgaag acggaatgcg cgatcgtggg cgcgatccaa tgctcgaaga
     9721 acgttagcgg gatctgatcg acgaccagga gacggcgaag gtgcgtggcg tggtcgtcct
     9781 tgagccccag ggagcgcgcc acctccgtcg gcgcagctgc ccactcgacg cccaaggttc
     9841 gcgcctcgac ggcatgcccc cgccgccgca gctcgctgcc gaagctctcc atctgccgga
     9901 gcgcgacccc gatcttcgcc gaggcgacga aggagccctt cccctgcgcc cggatgatca
     9961 ggccatcggt ctggagccgc cgcagcgcct gccgaacggg cgttcgactg gcctggaact
    10021 cgcgctggag ggcagcctcg gatggcaact ggcgtccctc ggtgagctcg ccgtcctcga
    10081 ttcggcgccg caggctgcgc tgaatcacct cgtgaatcgg cagtgaggaa tgctcgaaat
    10141 cattgcgcaa tggggccggt acctcatcgc gcagcatcgg ctaaggatac ttgggacagg
    10201 tagatactcg tcacgaaact tgttccaggg ctcggcagac gcgtcgccgc gtggtatcaa
    10261 ccggccagca tgacgaccgc gcacgttccc gagcccaggg gcgtcgtgct cgacctcgac
    10321 ggcacgctgg tcgacaccgt cgggacgcgg atcgatgctt ggctgcgaac gttcgctgag
    10381 gagggcatca cccccggtcg atccatcgtt gccgagctca tcggctcaga cgggcggcag
    10441 ctggcccgga ttgtgtttga cgcggccggc attgaactcg acgacgaacg cgcagagcgc
    10501 atcgacgccc gctccggcgc catctactcg gagctcaacg tcgacccgca accacttccg
    10561 agcgtcacgg gcttcctcga cgcgctggac aagctgaaga tcccctgggc gatcgcaaca
    10621 tcaagccggc gcgagcaagt cggccgatcg gttgcggcgc tccatcgtca cgagccgcct
    10681 ctcatcgccg atggcaccgc cgttcgcctg gcgaagcctc atcccgacct gctgctggca
    10741 gccgccggcg ccctcgaggt gcatcccgcc gaatgctggg cggtcggcga ctccacctgg
    10801 gacatggtcg cggcagaggc cgccggcatg gtggccatcg gcatcacggc cggctcagcc
    10861 atgagtccag accagctccg ggccgcgggc gccgcggtcg tcttccccac catggcccgc
    10921 ctcatccccc acctgagcag cggaacctag agtt
//
LOCUS       DATCQR010000221         2107 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_228288, whole genome shotgun sequence.
ACCESSION   DATCQR010000221 DATCQR010000000
VERSION     DATCQR010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228288"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            326..568
                     /locus_tag="VIN32_07425"
     CDS             326..568
                     /locus_tag="VIN32_07425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8922441.1"
                     /translation="MPGLLIVAATGPEDPTRASIPFHIACNGATPAGTQCAVAFAGDA
                     AELLKPDVIANVFGLGIPPLRDLLDKCVVQGIKFYV"
     gene            complement(883..>2107)
                     /locus_tag="VIN32_07430"
     CDS             complement(883..>2107)
                     /locus_tag="VIN32_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY8922442.1"
                     /translation="VWEVLLEDEPRHAPTHELDPEDPLMIIYTSGTTGKPKGTVHVHG
                     GFPVKTAQDMAHGFDVGAEDTIFWYTDIGWMMGPWLIFGSLALGATMVLYEGTPDHPA
                     PDRLWRMVDDHKVTVLGVSPTLVRGLMTHGDELPARHDLSSLRILGGTGEPWNPEPFK
                     WFFEKIGGGRLPIINYTGGTEISGGILCGNVITHLRPCSFAGPLPGMAADVVDAEGNS
                     VRGEVGELVIRNPWPGMTRGFWGDKQRYLDTYWSRFDGVWVHGDWAYADPQDDLWYVL
                     GRSDDTIKVAGKRLGPAEVESVLVAHSSVAEAAAIGVPDELKGEVLVCFVILRKGKAP
                     SEELAHELSDLVATALGKPLRPKAVHFVTDLPRTRNAKILRRVVRSVYTGKDPGDLSS
                     LENPSALAAIGATRD"
BASE COUNT          339 a          711 c          703 g          354 t
ORIGIN      
        1 aaaacggtca ccttgaccat tgcgtcacct cctggtgatc accccgcctg gggctcttac
       61 agaatcaacc agaccatgtc acttatcggt accgccgtca atccgggcca gagctgtgtc
      121 aggcggtgtg gaaggaggcc aaccgtgacc ccagattgag ccggcggctt aggaaggacg
      181 cgacgtgaag cgcgtcgtgt tctcggcggc accgccagca aggtcaggcg caggtcgatg
      241 cccccgagcc agagcaggag gaggcggacg aggtccttgc gggtgagctt cactctggcc
      301 tcatgaagtg cgcatactgg cacctgtgcc aggcctgctg atcgtcgccg ccaccggacc
      361 cgaggacccg acgcgcgcca gcatcccgtt ccacatcgcc tgcaacggcg ccacgcccgc
      421 tggtacccag tgcgcggtcg cgttcgccgg cgatgcagcg gagcttctca agcccgacgt
      481 cattgccaat gttttcgggc tcgggattcc gccgctgcgc gatctcctgg acaaatgcgt
      541 ggtccagggc atcaagttct acgtttgaaa aggctgtgct gaggcccgtg gggtcttgcc
      601 ggagatgctc accgagaatg catcgttcat cacgccgccc gatctggttc gcctgaccat
      661 ggaggcggac aaggtcctca gcttctgagg tgccccggcg ccggaggtcc gcgcatcttc
      721 gcggcctagt cggtcgggcg cctgccgcgc tgaagcctgc ggaaaggggc ccgacgccgc
      781 gtcgtcacgc atctggcgat gcgctcctag ctgcgccacc ctttcctttg gctccgaccg
      841 ccgatgccac gaatctgccg cggacgtcgg cgccggggca cctcagtcgc gggtggcacc
      901 gatcgctgcc agtgcgctgg ggttctcgag cgaagacagg tcgccaggat ccttgcccgt
      961 gtagaccgag cgcaccacgc gacgcaggat cttggcgttg cgggtcctgg gcaggtcggt
     1021 cacgaagtgg actgccttcg gccgcagcgg cttgccaagc gcggtggcga cgaggtcaga
     1081 gagctcgtgc gctagctcct cgcttggggc tttgcctttt cggagaatga cgaagcagac
     1141 gagcacctcg cccttgagct catcgggcac gccgatcgcc gccgcctccg ccaccgaaga
     1201 gtgcgcgacc agcaccgact cgacctcagc cgggcccagc cgcttgcccg cgaccttgat
     1261 ggtgtcgtcg gagcggccga ggacgtacca gaggtcgtcc tgggggtcgg cgtacgccca
     1321 gtcgccgtgg acccacacgc catcgaagcg gctccagtag gtgtcgaggt agcgctgctt
     1381 gtcgccccag aagccgcgag tcatccccgg ccatgggttg cggatcacga gctcaccgac
     1441 ctcgccgcgc accgagttgc cctcggcgtc gaccacgtcg gccgccatcc ccggcaaggg
     1501 tcccgcgaac gagcacggcc gcaggtgcgt gatgacgttg ccgcacagga tgccgcccga
     1561 gatctcggtg ccgccggtgt aattgatgat cgggagccgg ccgccgccga tcttctcgaa
     1621 gaaccatttg aacggctcag ggttccatgg ctcgccggtg ccgccgagga tgcgcaggct
     1681 cgacaggtcg tggcgcgccg gcagctcatc gccgtgagtc atcaggccac gaacgagagt
     1741 gggggagacg ccgagcactg tcaccttgtg gtcgtcgacc atgcgccaga ggcggtccgg
     1801 cgcggggtgg tcgggggtgc cttcgtacag gaccatcgtc gcgcccagcg cgagcgaccc
     1861 gaagatgagc caggggccca tcatccagcc gatgtcggtg taccagaaga tcgtgtcctc
     1921 ggcgccaacg tcgaaaccgt gagccatgtc ctgcgcggtc ttgacgggga agccgccgtg
     1981 cacgtgcacc gtgcccttcg gcttgccggt ggtccccgac gtgtagatga tcatcagcgg
     2041 gtcctcgggg tcgagctcgt gcgtcggcgc gtgacgcggc tcgtcctcca gcagcacctc
     2101 ccacacc
//
LOCUS       DATCQR010000222        13115 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_90062, whole genome shotgun sequence.
ACCESSION   DATCQR010000222 DATCQR010000000
VERSION     DATCQR010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 13115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13115
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90062"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..690)
                     /locus_tag="VIN32_07435"
     CDS             complement(<1..690)
                     /locus_tag="VIN32_07435"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF014611.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEY8922443.1"
                     /translation="MQVLRGVSELRAAILERRLAGDSVGFVPTMGVLHEGQLSLVRRA
                     SAENGCVVVSSFVNAGEFPTLDESSPMRRDEERDVALLERDGASLVFLPQVSTLYPPD
                     DVTRVSVEGLTRQLEGASRPGHLDGLTTVMLKMLNLVGPASVYLGQRHAQQIVILRRM
                     MRDLFMNIELVICPTIREADGLAVSSANASLSIEERHAATCLFAALNAARAAYDGGER
                     SAEVLRARMTEL"
     gene            complement(693..1961)
                     /gene="coaBC"
                     /locus_tag="VIN32_07440"
     CDS             complement(693..1961)
                     /gene="coaBC"
                     /locus_tag="VIN32_07440"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEY8922444.1"
                     /translation="MTEDRGSLAGRRVVVGVSGSIAAYKAVSLVRLLAERGAIVDVAM
                     TPAAAEFVTPLTFASLTHRRVVDDVMALDADSQIAHVELAEGADAIVIAPATANLLAE
                     LAAGNAGNAVTAIALASRAPLVVAPAMDAGMWTHPATQRNIETLRGFGHRIVEPEVGA
                     LASGLTGVGRLAEPDTIVEAVERLFARAGDLEGLQVVVTAGGTREPIDPVRFIGNRSS
                     GKMGVAIAEAARDRGASVTLIAGVMSVAPPRGVSVVDATTAAAMRQAVLRSASGADVL
                     VMAAAVADFAPKRASSSKIKRGGGELQIDLVANPDIIAEVGEMADGTRPFLVGFAAES
                     DELEANARSKLRDKGLDLIVGNKVGGPFDAIGSDENKVIVFGAEGELSDWPMLPKRQV
                     AERLWDLIVDRYRKAHAPERPERPARSRRR"
     gene            complement(1958..2803)
                     /locus_tag="VIN32_07445"
     CDS             complement(1958..2803)
                     /locus_tag="VIN32_07445"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF009855.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HEY8922445.1"
                     /translation="MKLLAADVGNTNITLGLFTDGELIGSWRSTAHATATPDEVATMT
                     AGMLALDGHGLDELQAVALASVVPPLTDTFERFVRTRIGLEPIMVDAASLAGTLKIEI
                     DRPAEAGADRLCNALAARTEFGGPAIVIDLGTSTNFDLLSAEGAYIGGAIAPGLGLSL
                     EALVGYASKLPRIELRRPPHAIGTNTINAMQSGTVLGYIGLVSGLLTALRGELLERSP
                     ADCRVTVIATGGYTHEPWLRDVPGIDAIEPDLTLRGIRYAYDAIREASTKASGAAAAG
                     TSGDR"
     gene            2904..3371
                     /locus_tag="VIN32_07450"
     CDS             2904..3371
                     /locus_tag="VIN32_07450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8922446.1"
                     /translation="MTDARAKLYINTGVVTDKSDIYNILRPLLKQTVTYTYRRGMQVT
                     GSGTGWVERIVVDHPEIASYFTPLAICLNLDSFDYLQFDTTSDQLLVYTLVIGEERVV
                     LEFATSAPRADVTDETDEAHPLPRSAGELARDLRFIQMELLMSPEASDDDEEA"
     gene            3373..4698
                     /locus_tag="VIN32_07455"
     CDS             3373..4698
                     /locus_tag="VIN32_07455"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEY8922447.1"
                     /translation="MRSGATAGRVRARASLPYQRSVLPNGLRVVTQQMPAARSVAVGL
                     FVGVGSRSEDEAHAGLSHLLEHLAFKGTRRYPEPGGLSEAVEGSGGSVNAATDRELTI
                     YTARVPAEAASRALEVVADLVLAPLLRTRDLIAEKPVIVDEIRMYVDSPGDHVFSLFD
                     ELLFGAHPLGREIAGTPRSVRRARHDTVVDHWQRWYRPSNLVLTAAGSIRHDQVVKAA
                     ERWFEADGPETVPVHLHPALERPRPAPAGSVRVAFRRLAQGNLCIGMPGVARDDPDRW
                     ALDLLGAVLGDGMSSRLFVELRERRSLSYDVSTFGSTYADCGTFGVHAGFDPEQAPAL
                     IAAIMEQLERVAQEPVSATELDRARAYTRGRLELRMEETGAVASWLGTGESLLPRILT
                     VDEVIERLEAVTVDDLLRVARTYVRPDLARLAVLGPFRSRLRFERMLQA"
     gene            4695..5486
                     /locus_tag="VIN32_07460"
     CDS             4695..5486
                     /locus_tag="VIN32_07460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8922448.1"
                     /translation="MTRRSPAATTGWDAPLEVVDLIPDELALARAQLESGLYGLAEGV
                     LLRLIAGLEAAGKGGLEELDGARALLAEALWRQGRPIAAGTAIEAIRSTSLERRRPML
                     MLIEAETVAAAGDPDRAARLAERVVSTVGVDEAWKLRGGVASRITWPAPSSFRLPARR
                     KDGAGSLGEAPPPAPTPERTAAAHTRLEAARRSFASGELDDGDRQLAAALRLDHRIAP
                     EGAVILEPTLGEEALAERLLLYGDLLRAAGREADATIAFDRAARS"
     gene            5511..5978
                     /gene="ndk"
                     /locus_tag="VIN32_07465"
     CDS             5511..5978
                     /gene="ndk"
                     /locus_tag="VIN32_07465"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEY8922449.1"
                     /translation="MERTLVLIKPDGVQRALVGEIVGRFERKGLKLVGLRMLDVSRAL
                     AEQHYQVHAGKHFYPGLVDFITAGPVAAVALEGPDAIATVRRMVGSTMPNEAEPGTIR
                     GDLGISGLRNLVHASDAVETAAAELALWFDPEDLVDYGRAIDSWIVAEEPARS"
     gene            complement(5924..6643)
                     /gene="lepB"
                     /locus_tag="VIN32_07470"
     CDS             complement(5924..6643)
                     /gene="lepB"
                     /locus_tag="VIN32_07470"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEY8922450.1"
                     /translation="MTYTPPPTAGPDLPAGGEPAAPRRRMGCALEIVETLVLTLVIYL
                     VIHNFVAQPFEVEQESMMPTVNPGEYVLIDKISPRFNDYQRGDIVVFQPPEGFGQGGV
                     PFIKRVIGMPGDTISLENGRVFVQQPGGSPVRIEEPYVRRTIDGGVAPTLPKDAEGTS
                     SWTVPAGTYFVMGDNRPASQDSRFFGVVDRELIVGRAWLRYFPLDRVGFVGHPTYAGL
                     TAAVYERAGSSATIQLSMARP"
     gene            6696..8777
                     /locus_tag="VIN32_07475"
     CDS             6696..8777
                     /locus_tag="VIN32_07475"
                     /inference="COORDINATES: protein motif:HMM:NF024603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEY8922451.1"
                     /translation="MTKRAVPLRNMAVLGLGFAVLAAILYYASTVDGRGPTVVGISLT
                     QHLSGDAEQALTTTSIEVAFSEPVQHASAEGSFTITPAVRGAFSWSAASLTFTPAARL
                     PLRTEFEVSIGPGVRDRAGNAMSGAPERFGFATVGNPTVIGSDPADAATDLPLDAPIV
                     VDFSTLMDTASVEAAISVSPDAEMTLRWSRERLTVVPVAGWEANRSYSLTISDGAQDQ
                     AGTPLEDPFRLSFQTVAAGLTVETIVPSDAVAGVSVTTPIAIIFDQALDPQSVNGDLL
                     TVTPTVAGSLDVVAPSGATGLSDTGRRILRFQPSDQLDPNTTYQVTLGPGLLGADGTG
                     MPAGMSWSFTTGAPTETLSNQVVFLADRSGIANLWAMNPDGSNQRQLSAELSPVTSYA
                     IAPDGRAFLTGDGATVVWQRSDGTARRVLTDPGVVEFDAAYSPDGTLITFGRADPALG
                     SSLGLWMRDADGSDPRPIELPSGQLASPSASQSAPVPLLRAPRVSPDGTALAFVDEAG
                     TVDILDLELQQLATAPFVALSEPIWLADGSGVLVAGLPAASGTGPSPYRPHSAVAVLD
                     PASAELAANHMAALHVVRMDRIATSVRATAFGAGASRPAVDASGRYAFIRLEDSETGG
                     GSLWMASALNDLGEAIEVPGTGTGSAAFAPEPGSMVIGEQGRAGVWLLRLGSRQVQRL
                     TADGWQPSWLP"
     gene            8942..11131
                     /locus_tag="VIN32_07480"
     CDS             8942..11131
                     /locus_tag="VIN32_07480"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HEY8922452.1"
                     /translation="MQVHPIVWVIPVAGLAAIAFALYLAWDVLRSDIGTEAMQEIAGT
                     IFEGAVAFIRRQYRTIGLLALGGAVLIGLVIALVEGKEVADTSIFGTELGIRTGVAFL
                     VGAACSMASGIIGMYISVKSNLRTAAAARSSLVRAVQVAMRGGAVSGFLVVALSLLGV
                     WSIFALYGGFTHPEQAPFLIVGFGFGASFVALFAQLGGGIYTKAADVGADLVGKVEAG
                     IPEDDPRNAAVVADLVGDNVGDCAGRGADLFESTAAENIGAMILGVAVFRIAQSAGWP
                     HPEAWIFFPLVVRAFGLLATIVAIFFVRGREDEDPMNMLNRGYWVTTILSVGGLALTT
                     SIMMSTPGTSGANGIAPWVWFFGAGLVGLATSVAFVYITQYYTAGAWRPVKEIAEASK
                     TGPATNIISGMAVGFETTAVTAITIAIALFASHWLGTQALPDVPNGGIFGTAVATMGM
                     LMTTAYILAMDTFGPITDNAGGIAQFAHAEGKAREITDHLDAVGNTTKALTKGYAIAS
                     ASLAAFLLFSAYIDKINLIQTNRGGPLLESVNLADVNVFIAALIGAMLVYFFSSLAIR
                     AVGKTAQAIIFEVRRQFREMPGIMDYTQRPDYARVVDITTRSALRQMILPGVVAVATP
                     IVVGLLLRQEAVAGLLMVGTIAGVLLATVLNNGGGAWDNAKKFIEAGHLTDDEGNVLG
                     KGTDAHAAAVVGDTVGDPFKDTAGPSLHVLVKLLATISLVLAPLFIR"
     gene            11156..12001
                     /locus_tag="VIN32_07485"
     CDS             11156..12001
                     /locus_tag="VIN32_07485"
                     /inference="COORDINATES: protein motif:HMM:NF014703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEY8922453.1"
                     /translation="MDLVLQAALIGILQGLTEFIPVSSSAHLELAPWIAGWPANGLLS
                     SLSFDVFLHLGTLLALLGYFGRDWLRLLGAAVASVRQRRIGDDPDRRLAWLLIAATIP
                     AALIGFVGEDLINEVLHADNHGVRLAIAGFVVLGAVALWLADRLGSRRREIGALDAPS
                     ALAIGLSQALALLPGISRSGATISAGLALGLTRESAARFSFLLATPITLGAGLYGSRH
                     LFEVSHTGTEWLAIATAFVAAAVSGLLAIGFLLSWLRTRSVTIFSVYRLLFAGLIVVL
                     VIAGR"
     gene            12190..12651
                     /gene="greA"
                     /locus_tag="VIN32_07490"
     CDS             12190..12651
                     /gene="greA"
                     /locus_tag="VIN32_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEY8922454.1"
                     /translation="MNNKPVYLTVEGLAKLKAELNHLITVERPRVAARIHDAKLDGDI
                     TENAEYEDAKQEQSFLEGRIGTLEGQLKNAEVIEKTNGDKVGIGSKVVIRSEDGQDTF
                     TIVGSAEASPREGRISNESPVGAALMGHKKGDTVVVQAPAGASTFTVVSIH"
     gene            12666..>13115
                     /locus_tag="VIN32_07495"
     CDS             12666..>13115
                     /locus_tag="VIN32_07495"
                     /inference="COORDINATES: protein motif:HMM:NF014031.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEY8922455.1"
                     /translation="MFWADELAARLTGPQVVNDSKTPSGTVHVGSLRGPVILDVIVRA
                     LRARNLPTTLLYGVDDLDPMDAQAMLTPDATERWMGAPLANVPDPVGECHASYARHFA
                     ALFIDTFAGLGFHPDRYYWMSEIYARGEVDPFIRTALDRAEVVRDIYR"
BASE COUNT         1928 a         4586 c         4506 g         2095 t
ORIGIN      
        1 cagctcggtc atccgtgccc gcagcacctc ggccgagcgc tcgccgccgt cgtaggcggc
       61 tcgagcggcg ttgagggcgg cgaagaggca ggtcgccgca tgacgctcct cgatcgagag
      121 ggaggcgttg gcgctcgaca cggccaggcc atcggcctcg cggatggtgg ggcagatcac
      181 cagctcgatg ttcatgaaca ggtcgcgcat catccggcgc aggatcacga tctgctgggc
      241 gtggcgctgc cccaggtaca cgctggccgg acccaccagg ttcagcatct tgagcatgac
      301 cgtggtcagc ccgtccaggt ggccgggtcg tgaggctccc tccagctgtc tcgtgagccc
      361 ctcgacgctg acccgggtca cgtcgtcagg cgggtagagg gtcgagacct gcggcaggaa
      421 gacgaggctt gccccgtccc gctccagcag cgcgacgtcg cgttcctcgt cgcgccgcat
      481 cgggctcgac tcgtccaggg tcgggaactc gcccgcgttg acgaaggagc tgaccaccac
      541 gcacccgttc tcagcactgg cgcgtcgcac cagcgagagc tgcccctcgt ggaggacacc
      601 catggtcggc acgaagccga cggagtcacc ggcaaggcgt cgctcgagga tcgcggcgcg
      661 cagctcggag acgccgcgca gcacctgcac gatcagcgtc gacgcgagcg agccgggcgc
      721 tcggggcgct ccggggcgtg agccttgcga tatcggtcca cgatcaggtc ccagagccgc
      781 tcggcgacct gacgcttggg aagcatcggc cagtcgctga gctcgccctc ggcgccgaag
      841 acgatcacct tgttctcgtc ggagccgatg gcgtcgaacg gtccaccgac cttgttgccg
      901 acgatcaggt ccaggccctt gtcgcgcagc ttgctgcgcg cgttggcctc cagctcgtca
      961 ctctcggcgg cgaagccgac gaggaagggg cgcgtcccgt cggccatctc gccgacctcg
     1021 gcgatgatgt caggattcgc caccaggtcg atctgcagct cgccgccacc gcgcttgatc
     1081 ttgctgctgc tggctcgctt tggggcgaag tcggccacgg ccgcggccat caccaggaca
     1141 tcggcgcccg atgccgagcg aagcacggcc tgacgcatgg cggcggcagt ggtggcatcc
     1201 accacgctca cgccgcgtgg gggcgcgaca ctcatcactc ccgcgatcag cgtgacgctc
     1261 gcgccacgat cgcgagccgc ctcggcaatg gccacgccca tcttgccgct gctgcggttg
     1321 ccgatgaatc gcaccgggtc gatcggctcg cgcgtgccgc cggcggtcac cacgacctgc
     1381 agcccctcca ggtcgccggc ccgcgcgaag agccgctcga ccgcctcgac gatcgtatcg
     1441 ggctccgcca ggcggccgac gccggtcagc ccgctggcca gcgcgccgac ctccggctcg
     1501 acgatccggt gcccgaagcc tcgcagcgtc tcgatgttgc gctgggtcgc cgggtgggtc
     1561 cacatccccg cgtccatggc gggtgcgacc accagcggtg cgcggctggc aagagcgatg
     1621 gcggtcaccg cattgccggc gttgccggcc gccagctcgg ccagcaggtt ggccgtggcc
     1681 ggcgcgatca cgatggcatc ggccccctcc gccagctcga cgtgggcgat ctgcgaatca
     1741 gcgtcgagcg ccatcacgtc gtcgaccacg cggcggtggg tgagcgacgc aaaggtcaac
     1801 ggggtgacga actcggctgc ggcgggcgtc atggccacgt cgacgatcgc cccgcgctcg
     1861 gccagcagcc ggaccagtga gaccgccttg taggcggcga tgctgcccga cacccccacc
     1921 accacccgcc tcccggcgag ggagccccga tcctccgtca tcggtcgccg ctcgtaccgg
     1981 cggcggcagc gccgcttgcc ttcgtgctgg cctcgcggat cgcgtcgtat gcgtagcgga
     2041 tgccacgcag cgtgaggtcg ggctcgatgg cgtcgatccc gggaacgtca cgcagccatg
     2101 gctcgtgggt gtatccgccg gtcgcgatga cggtcacccg gcagtcggcc ggagagcgct
     2161 cgagcaactc gcccctcagg gccgtcagca gcccactgac caggccgatg tagcccagca
     2221 ccgtgccgct ctgcatggcg ttgatcgtgt tcgtgccgat cgcatgcggc ggccggcgca
     2281 gctcgatcct cggcagcttg ctcgcgtacc ccaccagcgc ctcgagcgag agcccgagcc
     2341 ccggcgcgat cgcgcctccg atgtacgccc cctcggcgct gagcaggtcg aagttggtgc
     2401 tcgtgcccag gtcgataacg atcgccggcc ccccgaactc ggtgcgcgcg gcgagggcgt
     2461 tgcagagacg atcggccccg gcttccgccg gccggtcgat ctcgatcttc aatgtcccgg
     2521 ccagggaagc ggcgtcgacc atgatcggct ccaggccgat gcgggtgcgg acgaagcgct
     2581 cgaaggtgtc cgtcagcggc ggcaccaccg acgcgagcgc cacggcctgc agctcgtcca
     2641 gcccgtggcc gtcgagcgcc agcatcccgg cggtcatggt ggcgacctcg tcgggcgtgg
     2701 cggtcgcgtg agcggttgag cgccacgagc cgatcagctc gccgtccgtg aagaggccga
     2761 gcgtgatgtt cgtgttgccg acatcggcgg cgagcagctt cacgcgccac atcctaccag
     2821 ccgttcggcg ccctcccgcg ccgtgagtcc ggtgctagac tcgcccgcga tccccaccgc
     2881 aaggcgcagc ccgtgagcac ccgatgaccg acgcgcgagc gaagctctac atcaacaccg
     2941 gcgtcgtcac cgacaagagc gacatctaca acatcctgcg cccgttgctg aagcagaccg
     3001 tcacctacac ctatcggcgc ggcatgcagg tcaccggcag cgggaccggc tgggtggagc
     3061 ggatcgtggt cgaccacccg gagatcgcca gctacttcac gccgctcgcc atctgcctca
     3121 acctcgattc gttcgactac ctgcagttcg acaccacctc ggaccagctc ctggtctaca
     3181 ccctcgtcat cggcgaggag cgggtggtgc tcgaattcgc cacctccgcg ccccgcgccg
     3241 acgtgaccga cgagacggac gaggcgcatc cgctgccccg ctccgccggt gagcttgcgc
     3301 gggacctgcg cttcatccag atggagctgc tgatgtcgcc agaggcgagc gacgacgacg
     3361 aggaggcctg agatgcggtc cggcgcgacc gccggacgtg tccgcgcccg ggcgagcctt
     3421 ccctaccagc gcagcgtgct gccgaatggg ctccgggtgg tgacccagca gatgccggcc
     3481 gctcgctccg tcgcggtggg gctcttcgtg ggggtcggct cgcgtagcga ggatgaggcg
     3541 cacgccggac tctcgcacct gctggagcat ctcgccttca agggaacacg gcgctacccc
     3601 gagcccggcg gcctgtcgga ggcggtcgag ggatccggcg gcagcgtcaa cgccgccacc
     3661 gatcgagagc tgacgatcta taccgcccgc gtcccggccg aggccgcatc ccgtgccctg
     3721 gaggtggtcg ccgatctcgt tctggcgccc cttcttcgca cgcgagacct gatcgccgag
     3781 aagccggtca tcgtggacga gatccgcatg tacgtcgact ctccgggcga ccatgtcttc
     3841 agcctcttcg atgagctgct cttcggcgcg cacccgctcg ggcgcgagat cgcgggcacc
     3901 ccgcgcagcg tgcgtcgggc gcggcatgac acggtggtgg atcactggca gcgctggtat
     3961 cggccgtcga acctggtgct gacggcagcc gggtccatcc gccatgacca ggtggtgaag
     4021 gctgcggagc ggtggttcga ggccgatggg cccgaaacgg tgccggtcca cctccatccc
     4081 gcgctcgagc ggccaagacc cgcgcccgct ggctcggtgc gggtcgcatt tcgccgtctc
     4141 gcacagggga acctgtgcat cggcatgccg ggggtggctc gcgacgatcc ggaccggtgg
     4201 gcgctcgacc tgctgggtgc ggtcctcggc gacgggatga gcagccgcct gttcgtggag
     4261 ctgcgcgagc ggcgttccct gtcctacgac gtgtcgacct tcggctccac atatgcggat
     4321 tgcggaacgt tcggcgtgca tgccggcttc gacccggagc aggcgccggc gctgatcgcg
     4381 gcgatcatgg agcagctcga gcgggtggcc caggagccgg tcagcgcgac cgagctcgat
     4441 cgggcacggg cctacacccg cggacggctc gagctgcgca tggaggagac cggcgcggtg
     4501 gcctcctggc tcgggacggg ggagagcctg ctgccgcgaa tcctgaccgt cgacgaggtg
     4561 atcgagcggc tcgaggcggt gacggtcgac gacctcctgc gcgtggcgcg cacgtacgtt
     4621 cggcccgatc tcgcccgcct cgcggtgctg ggcccattcc gttcgcggct ccgcttcgag
     4681 cggatgctgc aggcatgacg cgccgctcac cggctgccac cacgggctgg gacgctccgc
     4741 tcgaggtggt ggacctcatc ccggatgagc tggcactggc gcgtgcccag ctggagtccg
     4801 ggctgtacgg cctggcggag ggggtcctgc tgcgcctcat cgccggcctc gaggcggcgg
     4861 gcaagggcgg cctcgaggag ctcgacggcg cgcgcgccct gctggccgaa gcgctgtggc
     4921 gccagggacg gccgatcgcc gccggcaccg ccatcgaggc gatccgcagc accagcctcg
     4981 aacgacgccg gccgatgctc atgctgatcg aggcggaaac ggtggcggcc gccggagacc
     5041 ccgatcgcgc cgcccggctc gccgagcggg tcgtttcgac ggtcggcgtc gacgaggcat
     5101 ggaagctgcg cggtggcgtc gcgagccgga tcacctggcc agctccatcc tcgttccgcc
     5161 tgccggctcg acgcaaggat ggggcgggaa gcctgggaga ggcgcccccg cccgcaccga
     5221 ctcccgagcg aacggcggcg gctcataccc ggctcgaggc tgcccgccga tccttcgcga
     5281 gcggggagct cgatgacgga gatcgccagc tggcggcggc actccggctc gaccatcgga
     5341 tcgcgccgga gggagcggtg atcctggagc cgacccttgg ggaggaggcg ctcgcggagc
     5401 gtctgctact ctacggcgac ctcctgcgcg ccgccggtcg cgaggcagat gccaccatcg
     5461 ccttcgaccg ggcagcacgc tcctgaccct cgcacggagg cgacttccgc atggaacgca
     5521 cccttgtcct gatcaagccc gatggagtcc agcgggccct cgtgggcgag atcgtcggcc
     5581 gattcgagcg gaaggggctc aaactggtcg ggctgcgcat gctcgacgtg tcacgggcgc
     5641 tggccgagca gcactaccag gtccatgccg gcaagcattt ctaccccggc ctggtcgact
     5701 tcatcaccgc gggtccggtg gcggcggtgg cgctcgaagg gcccgatgcc attgcgaccg
     5761 tgcgaaggat ggtgggcagc accatgccca atgaggccga gcccggcacc atccgcggtg
     5821 acctcgggat cagcggcctg cgaaacctgg tccacgcctc cgatgcggtc gagacggcgg
     5881 ccgcggagct cgccctgtgg ttcgacccgg aggacctggt cgactacggg cgcgccatcg
     5941 acagctggat cgtggcggag gagcccgccc gctcgtagac ggctgctgtc aggccggcgt
     6001 aggtcgggtg ccccacgaag cctacccggt ccagcgggaa gtagcggagc caggcgcggc
     6061 ccacgatcag ctcgcggtcg accaccccga agaaccgaga gtcctggctg gctgggcggt
     6121 tgtcgcccat cacgaagtag gtcccggcgg ggaccgtcca cgagctcgtc ccctcggcgt
     6181 ccttgggcag cgtcggcgcg actccaccgt cgatcgtgcg cctcacgtag ggctcctcga
     6241 tgcgcaccgg gctgccaccg ggctgctgta cgaagacgcg accgttctcc aggctgatcg
     6301 tgtcgccggg catgccgatg acgcgcttga tgaacggcac gcctccctgc ccgaatccct
     6361 cgggtggctg gaagacgacg atgtcgcctc gctggtagtc gttgaaccgc ggcgagatct
     6421 tgtcgatcag gacgtactcg ccgggattga cggtcggcat catcgactcc tgctcaacct
     6481 cgaatggctg ggcgacgaag ttgtggatga ccaggtagat caccagcgtc agcaccagcg
     6541 tctcgacgat ctcgagggcg catcccatcc tgcgacgcgg ggctgctggc tcgccgcccg
     6601 ccggcaggtc ggggccggcg gtcggcggag gggtgtaggt catcgggctc cgtcaggtga
     6661 gggcgtggca ggcggcgcgt agcatagcgg gctcagtgac gaagcgcgcc gtgccactca
     6721 ggaacatggc ggtccttggc ctcgggttcg cggtgctcgc cgccatcctg tactacgcct
     6781 cgaccgtcga tggccgaggc cccacggtcg tcggaatctc cctgacgcag cacctcagtg
     6841 gcgacgcgga gcaggcgctg acgaccacca gcatcgaggt cgccttctcc gagccggtcc
     6901 agcacgcctc ggccgagggg tcgttcacga tcacgccggc ggtccgcggc gcattcagct
     6961 ggagtgcggc cagcctcacc ttcacgccag ccgctcggtt gccgctgcga accgagttcg
     7021 aggtctccat cgggcctggc gtgcgtgacc gcgccggcaa tgccatgtcg ggcgctcccg
     7081 agcgattcgg gttcgccacg gtcggcaacc ccaccgttat tggatcggac ccggccgatg
     7141 ccgccaccga cctgcccctg gacgctccga tcgtggtgga cttctcgacc ctcatggaca
     7201 cggcatcggt cgaggcggcg atcagcgttt cgcccgacgc ggagatgacg ctgcgctgga
     7261 gccgcgagcg cctcacggtc gtacccgtcg ccggctggga ggcgaatcgg tcgtattcgc
     7321 tgacgatcag cgacggtgcg caggaccagg cggggacgcc gctcgaggac ccgttccgac
     7381 tttcattcca aaccgtcgcg gcgggtctga cggtcgagac gatcgtcccc tccgacgcgg
     7441 ttgccggcgt ctccgtcacc acccccatcg ccatcatctt cgaccaggcg cttgacccgc
     7501 agagcgtgaa cggcgacctg ctgaccgtca cgccgacggt ggcaggctcg ctggacgtcg
     7561 ttgcgccctc gggggcgacc ggcctgtcgg acacaggccg gcggatcctc cgcttccagc
     7621 cgtccgatca gctcgacccc aacacgacct atcaggtcac gctcggaccc ggtctactcg
     7681 gcgccgatgg aacggggatg cctgctggca tgtcatggag cttcaccacc ggggctccca
     7741 ccgagaccct ctcgaaccag gtggtcttcc tcgcggaccg ctcggggatc gcgaacctgt
     7801 gggccatgaa tcccgatggg tcgaaccaac ggcagctgtc ggcggagcta tcgccggtca
     7861 ccagctatgc catcgcgccg gatgggcgtg ccttcctgac tggcgacggg gcgacggtcg
     7921 tctggcagcg ctcggacggc acggcccgtc gggtcctgac cgaccccggc gtcgtagagt
     7981 tcgatgctgc ctactcgccc gatgggacgc tgatcacctt cggccgtgca gaccccgcgc
     8041 tgggatccag ccttggcctc tggatgcggg atgcagatgg gagcgatccg cggccgatcg
     8101 agctgccatc ggggcagctc gcgtccccgt cggcgagtca gtcggctccg gtgccgctgc
     8161 tgcgtgcccc gcgcgtgtcc cccgatggga cggcgctcgc gttcgtcgac gaggcgggga
     8221 cggtcgacat cctggacctc gagcttcagc agctggcgac ggccccgttc gtggcgctgt
     8281 ccgagccgat ctggctcgcc gacgggagtg gggtgctcgt tgcgggcctg cccgccgcct
     8341 cggggaccgg gccgagcccc taccgtccgc attcggccgt cgcggtcctc gatccagcat
     8401 ccgctgagct cgcggcgaac catatggccg cactgcatgt cgtgcgcatg gaccgcatcg
     8461 caacctcggt cagggcgacg gcattcgggg caggtgcctc tcgcccggct gtcgatgcct
     8521 ccggtcgcta cgccttcatt cggctggagg actcagagac aggcggcgga agcctgtgga
     8581 tggcatcggc gctgaatgac ctcggtgagg cgattgaggt gccgggcacg ggaaccggct
     8641 cggccgcctt tgcgcccgag ccggggtcga tggtgatcgg cgagcagggc cgagcagggg
     8701 tttggctgct caggctgggg agccggcagg tccaacggct caccgccgac gggtggcagc
     8761 cgagctggct gccctgaaag ttgacccgtc gcgagacgcc gggctaccat accggcgctc
     8821 cgctggcgga cgcgctgacg cgaccctccg ccggcgtttc tgtgtgcgag atgacgcgat
     8881 acgcgagccg ggcgcgccct gaatacggcg accgaaccgc tcaacccaat tcggaggttt
     8941 catgcaggtc catcccatag tctgggtgat tccggtcgcc ggcctggccg cgattgcgtt
     9001 cgcgctgtat ctcgcctggg acgtcctgcg ctcggacatc ggaaccgaag cgatgcagga
     9061 gatcgccggc acgatcttcg agggagcggt ggcgttcatc cgacggcaat accgcaccat
     9121 cggtctgctg gcgctgggcg gggccgtgct gatcggcctg gtgatcgcac tggtcgaagg
     9181 caaggaggtc gccgacacca gcatcttcgg caccgagctc gggatccgga ccggagtcgc
     9241 cttcctggtc ggcgccgcct gctcgatggc gtcggggatc atcggcatgt acatcagcgt
     9301 caagtcgaac ctgcggaccg ccgccgccgc gcgcagcagc ctggtgcgag cggtgcaggt
     9361 cgcgatgcgt gggggcgccg tctcaggctt cctggtggtg gctctttcgc tcctcggcgt
     9421 gtggtcgata ttcgccctgt acggtggctt cacgcatccg gagcaggcgc cgttcctgat
     9481 cgtcggcttc ggcttcggcg ccagcttcgt ggccctcttc gcccagctgg gcggcgggat
     9541 ctacaccaag gcggcggatg tcggcgccga cctggtgggc aaggtcgagg ccgggattcc
     9601 cgaggacgac ccacgcaatg ccgccgtggt ggccgacctg gtgggggaca acgtcggaga
     9661 ctgcgccggt cgcggggcgg acctgttcga gtcgaccgct gccgagaaca tcggcgccat
     9721 gatccttggg gtcgcggtct tccgcatcgc gcagagcgcc ggctggccgc atcccgaggc
     9781 ctggatcttc ttccccctgg tggtccgcgc cttcgggttg ctggccacca tcgtcgccat
     9841 cttcttcgtc cgtggtcgcg aggacgagga cccgatgaac atgctgaacc gtggctactg
     9901 ggtaaccacc atcctgtcgg tcggcggcct ggcgctcacg acgtcgatca tgatgagcac
     9961 tcccggcaca agcggcgcga acgggatcgc gccatgggtc tggttcttcg gagccgggct
    10021 ggtcggcctg gccaccagcg tcgccttcgt ctacatcacg cagtactaca cggccggggc
    10081 ctggcggccg gtcaaggaga tcgccgaggc cagtaagacc ggtccggcca ccaacatcat
    10141 ctcgggcatg gccgtcggct tcgagacgac cgccgtgacc gccatcacca tcgcgattgc
    10201 cctcttcgcc agccattggc tgggcacgca ggccctaccg gacgtcccga acggcggaat
    10261 tttcggcacc gccgtggcca ccatggggat gctcatgacc actgcctaca tcctggcgat
    10321 ggacaccttc ggcccgatca ccgacaacgc gggtgggatc gcgcaattcg cgcatgccga
    10381 gggcaaggcc cgcgagatca cggaccacct tgatgcggtc ggcaacacca ccaaggcgtt
    10441 gaccaaggga tatgcgattg cctccgcatc gctggcggcc ttcctcctct tctccgccta
    10501 catcgacaag atcaacctga ttcagaccaa ccgcggcggt ccgctgctgg agtcggtcaa
    10561 cctggccgat gtgaacgtct tcattgccgc cctgatcggc gcgatgctcg tctacttctt
    10621 cagcagcctc gccattcgtg ccgtcgggaa gactgcacag gcgatcatct ttgaggtccg
    10681 gcgccagttt cgcgagatgc cggggatcat ggattacacc cagcgaccgg actacgcgcg
    10741 cgtcgtggac atcaccaccc gctcggccct gcgccagatg atcctgcccg gcgtcgtggc
    10801 ggtggcgacc ccgatcgtgg tcggcctgct cctccggcag gaggccgtgg ccgggctgct
    10861 gatggtcggc accattgcag gagtgctgct ggcgaccgtg ctgaacaacg gcggtggcgc
    10921 atgggacaac gccaagaagt tcatcgaggc gggccatctc accgatgacg agggcaacgt
    10981 ccttggcaag ggaaccgatg cccatgccgc ggcggtggtg ggcgacacgg tcggggatcc
    11041 gttcaaggac accgccggcc catcgctgca cgtgctcgtc aagctgctgg caaccatcag
    11101 cctcgttctc gcgcctctct tcatccgtta gcaaccgctc gagcacggga gtgccgtgga
    11161 cctcgtcctc caggccgcgc tgatcgggat cctgcagggg ctgaccgagt tcatcccggt
    11221 cagctccagc gcgcatctcg agctggcgcc gtggattgcc ggctggccgg cgaacgggct
    11281 gctgagcagc ctgtcgttcg atgtctttct ccacctgggc acgctgctgg ccctgctcgg
    11341 ctacttcggc cgtgactggc tgcgcctgct gggagcggcc gttgcaagcg tccgccagcg
    11401 ccgcatcggc gacgatccgg atcgccgtct cgcctggctc ctcatcgccg ccacgattcc
    11461 cgccgcgttg atcggcttcg tgggggagga cctgatcaac gaggtgctgc acgccgacaa
    11521 ccacggcgtc cggcttgcga tcgctgggtt cgtcgtgctg ggagccgtgg cgctgtggct
    11581 ggccgatcgg ctcggttcgc gcaggcgcga gatcggtgcg ctcgacgccc ccagcgcgct
    11641 ggcgatcggc ctcagccagg cgctcgccct gctgcccggc atcagccgat cgggagcgac
    11701 catcagcgcc ggcctggcgc tcggcctcac acgcgagtcg gccgcgcgct tcagcttcct
    11761 gctggccacg ccgatcaccc tgggcgccgg cctgtacggc tcccgccacc tgttcgaggt
    11821 gagccacacc gggaccgagt ggctggcgat cgccaccgcg ttcgttgccg ctgccgtgtc
    11881 ggggctgctg gccatcggct tcctgctgtc gtggctgcgc acccggtccg tcaccatctt
    11941 cagcgtgtat cggctcctgt ttgccgggct catcgtcgtg ctcgtgatcg cgggtcgctg
    12001 aggtcgaatc gagcctgcca gcagggcctc cagacctttg acagcgcccg gtggccgccc
    12061 tacaatccgg cgacgccgag cccatccgac gcagggaggg ccgcaagcga acctggccca
    12121 gggccagctt caggaacgcc ccgcgaaagc catggcggga cctggagcat tttttgttag
    12181 tggatgacca tgaacaacaa gcctgtctac ctgactgtcg aagggctggc caagctcaag
    12241 gcggagctga atcacctgat caccgtcgag cggccgcgcg ttgctgcgcg gatccatgac
    12301 gcgaagctgg atggcgacat caccgagaac gccgagtacg aggatgccaa gcaggagcag
    12361 tcattcctgg agggccggat cggcacgctc gagggtcagc tcaagaacgc cgaggtcatc
    12421 gagaagacca atggcgacaa ggtcggcatc ggctcgaagg tggtcattcg cagtgaggac
    12481 ggccaggaca cgttcaccat cgtcggctcg gccgaggcct ccccacgcga gggccggatc
    12541 agcaacgagt cgccggtcgg cgccgccctg atggggcaca agaagggcga taccgtcgtc
    12601 gtccaggccc cggctggagc atccaccttc acggtcgtca gcatccacta gccgcgcccg
    12661 atcaggtgtt ctgggccgac gagctggcag cgcgcctcac cgggccgcag gtcgtcaacg
    12721 actcgaaaac cccctcgggg accgtgcacg tcgggagcct gcgtggaccg gtcatcctcg
    12781 acgtcatcgt gcgggctctg cgtgcgcgca acctgccgac gaccctcctg tacggcgtcg
    12841 acgacctgga tccaatggac gcgcaggcga tgctcacccc cgatgccacc gagcgctgga
    12901 tgggggcgcc gctcgccaac gtaccggacc cggtggggga gtgccatgcg tcctatgccc
    12961 ggcacttcgc cgcgctcttc attgatacct tcgccgggct cggcttccat cccgaccgct
    13021 actactggat gagcgagatc tacgcgcgcg gggaggtcga ccccttcatc cgcaccgcgc
    13081 tcgatcgggc cgaggtggtg cgcgacatct accgc
//
LOCUS       DATCQR010000223         8698 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_121232, whole genome shotgun sequence.
ACCESSION   DATCQR010000223 DATCQR010000000
VERSION     DATCQR010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 8698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8698
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121232"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..681)
                     /locus_tag="VIN32_07500"
     CDS             complement(<1..681)
                     /locus_tag="VIN32_07500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8922456.1"
                     /translation="MSFLRRRNKEVPPPPPPTPFIEEITAQEYSLKLAYLARSSDGLR
                     LPADPRMKAMLPSVVEPLAEGPVEVLEPVSHELAEAAPTIERLPELQQWIEARSTVSP
                     IALHALYVLESTDAVDMTVDSLAVALLQGETDTSGYPDYAAIVGGLASHWDEISGDLI
                     VRAVVGWGGKGQRGDTERTARRLLSSLYQQVRATGHSLGVAAATRAATGPEHGGSVCR
                     HCGFESGTD"
     gene            complement(726..3401)
                     /locus_tag="VIN32_07505"
     CDS             complement(726..3401)
                     /locus_tag="VIN32_07505"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF004349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEY8922457.1"
                     /translation="MTTPRLELPPRYNAAEVEPAIYERWLSSGAFTPSAEQPPGAERF
                     VITMPPPNVTGSLHVGHALFVTLEDMLIRYHRMRGDDTLWLPGVDHAGIAAQFVLDKV
                     IAEEGETRESLGRERYLERMWRFIDETRDVIGLQMRRLGASPDWTRNQFTMDPMSARA
                     VRTAFKRLHDADLVYRGEALVNWCPRCRTTISDLEKVPKEETGTLWTIRYHLAREDGS
                     PDPDRWISIATTRPETLLGDVAVAVHPEDDRYRELVGREAILPFLARRLPIIADEVVE
                     RDFGTGAVKITPAHDPDDYELGRRHALPAINVLDEGAHVNEQGGPFTGLDRYEARSRI
                     VEQLREMGDLEDERPHAMAVGHCERCGTVIEPRLSVQWFVRTTPLAKRALASVREGRT
                     RIVPEHFTKVYAHWMENIRDWAVGRQLWWGHRIPAWYCPDGHITVSDLEAGPDACATC
                     GRPAAELTQETDIFDTWFSSGLWPFSTLGWPDDTPDMRRFYPTSVMETGYDIIFFWVA
                     RMMMLGLFLTDTEPFHTVYLHGLVRATGGIKMSKTKGNTADPLELIEEIGADALRFAL
                     TVGTSPGADQRLTEGKLEGARNFTNKLWNAARFVLGSRPEPTAPSEGDPTPAERWIRS
                     RLAEATERATRQLDDLELSAYAASAHEFAWTDYCDWYLEMAKVDLRRPGASDGEKARA
                     WRTASGALATMLQLLHPIVPFVTEEIWSRLVASDSDAAVRPELLITASWPGTEGRDRE
                     AESEMAVLIDLIRGVRNLRTESGVSAGARLPLTVVPADAAARAGIERGLDYLAALARV
                     GPIDLRAPGDDRGRPELVASTAGAAAWLGGEPSDSADGPSRQAANEAHLRRGIERLQV
                     LLRGDFATRAPAEVVERERSRLADLEAQLRLLTGG"
     gene            complement(3663..4811)
                     /locus_tag="VIN32_07510"
     CDS             complement(3663..4811)
                     /locus_tag="VIN32_07510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8922458.1"
                     /translation="MSAEETAFAGLAERTETSPGAEMVNGTSPNSSDGAEAPVVAHSS
                     PAMNDSGQEPAVPFLQSLVDAMRKIAEQTRDDAMAQLRAGVAERSAGVRSETEQRAAE
                     LHELANADLKSIEDWERGETQRIRDEAAVKVSARNATLEAQLAANTAAGEGATTAVEA
                     RVDAFEAEMSSFFAQLNDIHDPAALASRLKRIPQAPSLNDVAVAAAGDTAPSPSPAAA
                     EPNSAKAPDVMGVPKQAPAWTAARGNAAPQPASDAIEVPEAKPASETASAEATDGATT
                     DGATADAAEVASVEPEAVEPPVAESTTQVVVTGLTSFGAITSFKQSLEGVNGINRVSL
                     GLGTSGEFIFTAAHREGFDLSAAIQSFEDSAQFVTSNGQLRVTVGAKA"
     gene            complement(4817..6055)
                     /locus_tag="VIN32_07515"
     CDS             complement(4817..6055)
                     /locus_tag="VIN32_07515"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY8922459.1"
                     /translation="MNRPSVLLALDGWSSSYEDAMTDAGFEAVAADREEALAWLSGDG
                     QIDLAVIDCDRGQPGDEELFAFLHSTQRVPTIVLFTNDIPEWAVDANPGATRDEYARK
                     PIPPEALVYRLQAMLIHSGREVPGALPTTTPDGAEVETIGEGTVVSLFAPKGGVGKTM
                     IAVNLAVAIRQQTGDSVCLMDADVGVGNVTAVLNAPYHGGLAHLADSPPEEWTNDAFE
                     QTVTTHEASGVRVLTWGTEPSESERISTDLLVAAVRWARTHHSWVIIDCHPSYDDRTM
                     AMLAASREIFLVVTPEVGPLRNASQFLGLAREVGLGDMVKVIVNRSNHGISVDDIAKT
                     LGTRISATVVSNGPKAIAAANEGTPIIIKFPKEQMSTDLYNVARLLTRPVALEAPARR
                     RSWWSALVSPSNEAGKAVGR"
     gene            complement(6171..7466)
                     /gene="clpX"
                     /locus_tag="VIN32_07520"
     CDS             complement(6171..7466)
                     /gene="clpX"
                     /locus_tag="VIN32_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011194493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEY8922460.1"
                     /translation="MTGKTKVPYRCSFCGKSQEQVRKLIAGQGVYICDECINLCQEII
                     EEEMLEAPRAKTSPAALPSPRRIKEKLDSYVISQDQAKKVLSVAVYNHYKRVNAGSSV
                     DEVELGKSNVLLVGPTGSGKTLLAQTLAKVLDVPFCIADATSLTEAGYVGEDVENILL
                     RLIQAADFDVQRAQRGIIYIDEIDKIARKTDNPSITRDVSGEGVQQALLKILEGTVAN
                     VPPQGGRKHPHQDFIQIDTTNVLFICGGAFEGLEKIVEERVGKRGIGFGSQAQRSKEE
                     RRRLMDQLMPEDLLKYGLIPEFVGRLPVVVGLTALAKEDLIKVLTEPKNAVTRQFAKF
                     LSLDRVELVFTDEALEATARLAIDRKTGARALRTIVEEALLEVMYDIPERTDVKKVVI
                     TGETIEHGQPPMLVTETDDAEPRRRTPRPRPAERAEESA"
     gene            7537..8028
                     /locus_tag="VIN32_07525"
     CDS             7537..8028
                     /locus_tag="VIN32_07525"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005328612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEY8922461.1"
                     /translation="MDRSAVSHAPLDSPIGRLWVAVSARGLVSVTRGENPSPDTLAVN
                     LDPDAVAPVLTELERYFAGELRTFSTPLDLRGAGAFDAAVWSAARQIPYGETVSYGEL
                     AGMAGHPRAARAAGNAMARCPFSPVVPCHRVIHADGSIGGWGADTWVKRWLLDLERGA
                     SIV"
BASE COUNT         1279 a         2934 c         3006 g         1479 t
ORIGIN      
        1 gtcggtcccc gactcgaagc cgcagtgcct gcagaccgag ccgccgtgct caggaccagt
       61 cgctgcgcgg gtggcggccg ccacgccaag gctgtggccc gtggctcgaa cctgctgata
      121 gagagagctg agcaggcggc gggcagttcg ctcggtgtcc ccgcgctgcc ccttgccgcc
      181 ccagccgacc acagcccgca cgatcaggtc gccggagatc tcgtcccagt ggctggccag
      241 cccgccgacg atcgctgcgt agtctgggta gccgctggtg tccgtctcgc cctgcagcag
      301 ggcgacggcg aggctgtcga ccgtcatgtc gacggcatcg gtcgattcga gcacgtagag
      361 cgcatgcagt gcgatcgggc tgaccgtcga ccgcgcttcg atccactgct gcagctccgg
      421 aagtcgctcg atggtgggcg cagcctcagc cagctcgtgg ctgaccggct cgagcacctc
      481 gaccgggccc tccgccagcg gctcgacgac cgatggcagc atggccttca tgcgcgggtc
      541 cgccggcagg cgcagcccat cgctgctccg cgccaggtag gccagcttga ggctgtactc
      601 ctgggccgtg atctcctcga tgaagggggt cggcggaggc ggaggcggga cctccttgtt
      661 gcgtcggcgg aggaaggaca tcgaccgatt ctagcagcgg agccggcgaa ctcccgctgc
      721 atgcgtcagc cgccggtcag cagccggagc tgcgcctcca gatcggcgag tcgcgatcgc
      781 tcgcgctcga ccacctcggc cggggcacgc gtcgcaaagt ccccgcgtag caggacctgc
      841 aggcgctcga tcccgcgccg gagatgcgcc tcgttcgcgg cctgccggga agggccgtcc
      901 gccgagtccg agggctcccc tccgagccac gctgccgctc ccgcggtgga ggcgaccagc
      961 tcgggccggc ctcggtcgtc gccgggcgcg cgcaggtcga tcggtcccac ccgagccagg
     1021 gcggcaaggt agtcgagccc gcgctcgatg ccagctcgcg cggcggcatc tgccggcacc
     1081 acggtcagcg gcagccgagc gccggcgctc acgccggact cggtgcggag gttgcggacg
     1141 ccacggatca ggtcgatcag caccgccatc tccgattccg cctccctgtc gcgcccctcg
     1201 gtccccggcc agctggccgt gatgagcagc tccggccgca ccgccgcatc gctgtcggaa
     1261 gccaccaggc gcgaccagat ctcctccgtc acgaacggga cgatcggatg gaggagctgc
     1321 agcatcgtcg ccagcgcccc actcgccgta cgccacgccc gggccttctc gccgtcgctg
     1381 gcgccggggc ggcgcaggtc gaccttggcc atctccaggt accagtcgca gtagtcggtc
     1441 caggcgaact cgtgcgcgga ggctgcgtac gccgagagct cgagatcgtc gagctgtcga
     1501 gtggcacgct ccgtcgcctc ggccagtcgt gaacggatcc atcgctcggc cggcgtggga
     1561 tcgccttctg atggagccgt cggctcagga cgggagccga ggacgaagcg tgccgcgttc
     1621 cacagcttgt tggtgaagtt gcgcgccccc tccagcttcc cctccgtcag ccgctggtcc
     1681 gcgccgggcg aggtgccgac cgtcagggcg aagcgcagcg catcggcccc gatctcctcg
     1741 atcagctcga gcggatctgc cgtgttcccc ttggtcttgc tcatcttgat gccgccggtg
     1801 gcgcgcacca acccgtgcag gtagaccgtg tggaacggtt cggtatcggt caggaagagg
     1861 ccgagcatca tcatgcgggc gacccagaag aagatgatgt cgtagccggt ctccatcacc
     1921 gatgtcggat agaagcgccg catgtcgggc gtgtcgtccg gccagcccag ggtgctgaaa
     1981 ggccacaggc cgctgctgaa ccaggtgtcg aagatgtccg tctcctgggt cagctcagct
     2041 gccggccggc cacacgtggc acaggcgtcc gggcccgcct cgaggtcgct gaccgtgatg
     2101 tgcccgtcgg ggcaatacca ggccgggatc cgatgccccc accacagctg ccgccccacc
     2161 gcccagtcgc ggatgttctc catccagtgc gcgtagacct tggtgaagtg ctccggcacg
     2221 atccgcgtcc gaccctcgcg caccgaggca agtgcgcgct tggcaagtgg cgtggtgcgc
     2281 acgaaccact gcaccgacag gcgcggctcg atgaccgtcc cgcagcgctc gcagtgtccg
     2341 accgccatgg catgtggtcg ctcgtcctcc aggtcgccca tctcgcggag ctgctcgacg
     2401 atccgtgacc gcgcctcgta gcgatccagg cccgtgaacg gcccgccctg ctcgttgacg
     2461 tgcgctccct cgtccagcac gttgatcgcc ggcagcgcgt ggcggcggcc cagctcgtag
     2521 tcgtcggggt cgtgggcggg cgtgatcttg accgccccgg tcccgaagtc gcgctcgacc
     2581 acctcgtcgg cgatgatcgg caggcggcgt gccaggaacg gcaggatcgc ctcgcgaccc
     2641 accagctcgc ggtagcggtc gtcttcggga tgaactgcca ccgccacgtc acccagcagc
     2701 gtctccgggc gggtggtggc gatcgagatc catcggtccg gatcgggtga gccatcctcg
     2761 cgggccaggt gatagcggat cgtccacagc gtgccggtct cttccttcgg caccttctcg
     2821 aggtcggaga tggtcgtccg gcagcgcggg caccagttga cgagtgcctc gccgcggtac
     2881 accaggtcgg cgtcgtggag ccgcttgaac gcggtccgca cggcgcgggc gctcatcggg
     2941 tccatggtga actggttgcg ggtccagtcg ggggaggcgc caaggcgacg catctggagg
     3001 ccgatgacgt cgcgcgtctc gtcgatgaag cgccacatgc gctccaggta gcgctcgcga
     3061 ccaagtgact cgcgcgtctc cccttcctcg gcgatgacct tgtcgagcac gaactgggcc
     3121 gcaatgccgg cgtgatcgac cccaggcagc cacagcgtgt cgtcgccgcg catgcggtga
     3181 tagcggatca gcatgtcctc gagggtcacg aacagcgcgt ggcccacgtg cagcgagccg
     3241 gtcacgttcg ggggcggcat cgtgatcacg aagcgctctg ccccgggcgg ctgctcggcg
     3301 gatggcgtga aggcgccgct cgacagccat cgctcataga tcgcgggctc gacctcggcc
     3361 gcgttgtagc gcggcggaag ctcgagtcgc ggcgtcgtca tcggtcatgc ctccgtggca
     3421 acaacaaacc cgttcgtcga aggacgaacg ggtcgtggta ccaccttcat tcggcgacat
     3481 cggccgatcc gatggcgccc tcgttaccgc ggtatcgggc ggtacccggc cggctcgcgt
     3541 gcgaccttcg ccctctcgtg ccgaggcggg cttccagcct gtggcccacc atctctcacg
     3601 gtcgtcggag gggtactcct cacggtcgtc gccgtgcttc ggagtctacc acgaggccga
     3661 tctcaggcct tggcgcccac cgtgacgcgc agctgaccgt tggaggtgac gaactgcgcc
     3721 gaatcctcga agctctggat cgcggccgac aggtcgaatc cctcccggtg ggcggcggtg
     3781 aagatgaact cgccggaggt cccaaggccc agcgacactc gattgatgcc gttcacccct
     3841 tcgagggact gcttgaacga ggtgatggca ccgaagctgg tcaatccggt gacgaccacc
     3901 tgggtggtgc tctctgcgac aggcggctca accgcctcgg gctccacgga cgcaacctcg
     3961 gcagcgtcag ccgtggcacc gtcagtcgtg gcaccgtcag tcgcctcggc cgatgctgtc
     4021 tcggacgccg gcttcgcttc ggggacctcg atggcgtcgc tcgcaggctg gggggccgcg
     4081 ttgccacgag cggcggtcca ggccggggcc tgcttgggca ctcccatgac atcgggagcc
     4141 ttggctgaat tgggttccgc tgccgcgggc gacggcgacg gggccgtgtc tcccgcggct
     4201 gccaccgcaa catcgttcag cgatggcgcc tggggaatgc gcttcagcct cgaagcgagg
     4261 gccgcggggt cgtggatgtc gttcagctgc gcgaagaagg agctcatctc cgcctcaaag
     4321 gcgtcgacgc gtgcctcaac cgcggtggtg gcgccttcgc cggcggcagt gttggcggcg
     4381 agctgcgcct cgagggtcgc attgcgagcc gagaccttga cggcagcctc atcgcggatc
     4441 cgctgagtct cgccgcgctc ccagtcttcg atgctcttca ggtcggcatt ggcgagctcg
     4501 tggagttcgg cagcgcgctg ctcggtctcg gagcggacgc ccgctgaacg ctcggcgacg
     4561 cctgcgcgga gctgagccat cgcgtcgtcg cgcgtctgct cggcgatctt gcgcatcgcg
     4621 tccaccaggc tctgcaggaa gggcacggct ggctcttggc ccgagtcatt catcgctggc
     4681 gaggagtgtg ccacgacggg agcctccgca ccatccgaag agttaggcga cgtgccgttg
     4741 accatctcag cgccggggct cgtctctgtc cgctcggcca gcccggcgaa ggcggtctcc
     4801 tccgctgaca tctcgctcac cgcccgactg cctttccggc ctcattcgac ggcgagacca
     4861 gtgcggacca ccacgagcgg cggcgcgccg gcgcctcgag ggccacggga cgggtcagca
     4921 ggcgggcaac gttgtacagg tcggttgaca tctgctcctt ggggaacttg atgatgatcg
     4981 gcgtcccctc gttcgccgcg gcgatcgcct tggggccatt cgagaccacg gtggcgctga
     5041 tgcgcgtccc cagggtcttg gcgatgtcgt ccacggagat gccgtggttg gagcggttga
     5101 cgatcacctt gaccatgtcc ccgagcccga cctcgcgggc aaggcccagg aactgcgagg
     5161 cgttgcgcaa cgggccaacc tcgggggtga ccaccaggaa gatctcgcgc gacgcggcca
     5221 gcatggccat ggtgcggtcg tcatagctgg gatggcagtc gatgatcacc cagctgtggt
     5281 gggttcgcgc ccagcgcacg gcggcgacca gcaggtcggt gctgatccgc tcggactcac
     5341 tcggctcggt tccccaggtc aggacccgga cgccgctggc ctcgtgggtc gtgacggtct
     5401 gctcgaaggc atcgttggtc cactcctcgg ggggcgagtc ggccaggtgc gccagtccac
     5461 cgtggtacgg cgcgttgagg acggcggtca cgttgccaac gccgacgtcg gcgtccatca
     5521 gacagacgct gtcgccggtc tgctgccgga tcgcgacagc gaggttgacg gcgatcatcg
     5581 tcttgccgac gccgcccttg ggggcgaaga ggctcacgac ggttccctca ccgatcgtct
     5641 cgacttccgc accatcgggg gtggtggtgg gcaaggcgcc gggcacctcg cgcccgctgt
     5701 ggatcagcat cgcctggagc cgatagacga gcgcctccgg cggaatcggc ttgcgagcgt
     5761 attcgtcgcg ggttgcgccc gggttcgcgt cgaccgccca ttcggggatg tcgttggtga
     5821 agagcacgat ggtgggaacg cgctgcgtcg agtgcaggaa ggcgaacagc tcctcgtcgc
     5881 ctggctgacc acggtcgcag tcgatgacgg cgagatcgat ctggccgtcg ccggagagcc
     5941 aggcaagggc ctcctcgcga tcggcggcga ccgcctcgaa tccggcgtcg gtcatcgcgt
     6001 cctcgtagga ggagctccac ccatccaggg cgagcaggac gctgggtcgg ttcatgtgca
     6061 gtgcctctgg gtgtggtgcg gtgacgagtg gcaccacaat ccggcgagcg ggcacttcgg
     6121 gcaatgggac ctcggcacca tcggtgcccg ccgaaggtac ggggaggtcc ctatgcggac
     6181 tcttcggcgc gctccgctgg ccgcgggcgg ggggtccgcc ggcgaggctc ggcgtcatcg
     6241 gtctcagtga ccagcatcgg cggttggccg tgctcgatcg tctcgccggt gatcaccacc
     6301 ttcttcacgt cggtccgctc ggggatgtcg tacatgacct cgagcagcgc ctcctcgacg
     6361 atcgtccgca gggctcgcgc cccggtcttg cgatcgatcg ccaggcgcgc ggtggcctcg
     6421 agtgcctcat cggtgaagac aagctccacg cggtcgagcg acaggaactt ggcgaactgg
     6481 cgggtgacgg cattcttcgg ctcggtcagg accttgatca ggtcctcctt ggccagggcg
     6541 gtcagaccga ccacgactgg gaggcgaccg acgaactccg ggatgagccc gtacttgagc
     6601 aggtcctcgg gcatcagctg gtccatcagc cggcgccgct cttccttcga gcgctgcgcc
     6661 tgcgagccga agccgatccc ccgcttgccg acccgctcct cgacgatctt ctcgaggccc
     6721 tcgaacgcgc caccacagat gaacaggaca ttggtggtgt cgatctggat gaagtcctgg
     6781 tgcgggtgct tgcggccgcc ctgcggcggg acgttggcga ccgttccttc caggatcttg
     6841 aggagcgcct gctgcacgcc ctcgcccgag acgtcgcggg tgatggaggg gttgtcggtc
     6901 ttgcgggcga tcttgtcgat ctcgtcgatg tagatgatcc cgcgctgcgc ccgctgcacg
     6961 tcgaagtcgg cggcctggat caggcgcagc aggatgttct caacatcctc gccgacgtag
     7021 ccggcctccg tcaggctggt ggcatcggca atgcagaagg gcacgtccag gaccttggcc
     7081 agcgtctggg ccaggagggt cttcccgctg ccggtggggc cgacgagcag gacgttgctc
     7141 ttgccgagct cgacctcgtc gaccgaggag cccgcgttga cccgcttgta gtggttgtag
     7201 accgcgacgg agaggacctt cttggcctgg tcctggctga tgacgtagct gtccagcttt
     7261 tccttgatcc ggcgcgggga cgggagggcg gcgggggagg tcttggcgcg aggcgcctcg
     7321 agcatctcct cctcgatgat ctcctggcag aggttgatgc actcgtcgca gatgtagacg
     7381 ccctggcccg cgatgagctt gcgcacctgc tcctggctct tcccgcagaa ggagcagcga
     7441 tagggaacct tggtcttgcc ggtcattgct tcgagcatag cacgcgcttt cgagcgtgcc
     7501 ggctatagac cgatggtccc cggtagcatc cccccggtgg atcgctccgc cgtctcccat
     7561 gcgcctctcg actcaccgat tgggcggctg tgggttgccg tctcggcccg cggcctggtc
     7621 tcggtgacgc ggggcgagaa cccctccccg gataccctcg ctgtgaacct cgatccggac
     7681 gcggtggccc ctgtcctgac ggagctggag cggtacttcg cgggagaact gcggaccttc
     7741 tcgactccgc tcgacctgcg gggcgccggc gcgttcgacg ccgccgtctg gagcgccgct
     7801 cgacagatcc cttatggcga gacagtctcc tatggggagt tggccggcat ggctggccat
     7861 cctcgcgcgg cccgcgccgc cggcaacgcc atggcccgct gcccgttctc gccggtggtg
     7921 ccatgccata gggtcatcca cgccgacggc tcaatcggtg gctggggcgc cgatacctgg
     7981 gtcaagcgct ggctgctcga cctggagcgc ggcgctagca tcgtttgatg gctacccagg
     8041 ctgaggtcaa ccttgcgggt ttgcacggta gtgcatcagt ttgagccgca ggagtaggct
     8101 ggtggcctag ggatcagtag tcgcagaagg gagcggtcag accatgcggg gagtcgaatg
     8161 tgcagcgccg aactgcgcgc acctgcacgc tgaggatgac gaggagctag tgcagctagc
     8221 gatgcgtcac gcggaggaag tgcatccaga catgcctctc ccagagtcgg cggctcgtga
     8281 gttcgtgcaa aacggtgcct atgacgatac tcagcacgcg ggcggcagca ctagcggcta
     8341 gttgagcagg tccgcgatct cctctgatgt cctcggcctg gcggccgcca gaggtcaacc
     8401 tatcgggttt gcaggtagct gagctatggc gagcgcacca gaacccaagt cctaagagcc
     8461 catgcggcga atgcgccgga cccgatagag aggcctatcc aaagctcggg gcttgatggg
     8521 cccgagccaa tcggatgtca ggaacatgac gccgccgccg accgcgccaa acgtggcgat
     8581 caccgcgagt ccccaaagct cgttttgcac ggcgcgaatc agtctggact tcatgctcct
     8641 aggcaagcag gtccgcgatc tcctcgcagg gcccttgacc tgccgacgaa gggcgcgc
//
LOCUS       DATCQR010000224         1999 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_90303, whole genome shotgun sequence.
ACCESSION   DATCQR010000224 DATCQR010000000
VERSION     DATCQR010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1999
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90303"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..452
                     /locus_tag="VIN32_07530"
     CDS             <1..452
                     /locus_tag="VIN32_07530"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF012441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="orotidine 5'-phosphate decarboxylase / HUMPS
                     family protein"
                     /protein_id="HEY8922462.1"
                     /translation="LGEDSVEPFLAYPDRLVYLVARTTNPSAGTLQDLPVGGTPLHEH
                     VATWAAGRWTDGRVGLVVGATAPAELARLRRLVPALGFLVPGVGAQDGDLAAALENCH
                     GTAAPGLVSISRGIAGASQGDGWRAAAAASAQSWLGRMQEAGATLPA"
     gene            504..719
                     /gene="tatA"
                     /locus_tag="VIN32_07535"
     CDS             504..719
                     /gene="tatA"
                     /locus_tag="VIN32_07535"
                     /inference="COORDINATES: protein motif:HMM:NF011430.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEY8922463.1"
                     /translation="MGPIGWPELIILLVVVLIVFGPGKLPDIGNAIGRGVKEFRKASN
                     DLESSIRGEPSKPAPPPPATTDDKPQA"
     gene            774..1484
                     /gene="tatC"
                     /locus_tag="VIN32_07540"
     CDS             774..1484
                     /gene="tatC"
                     /locus_tag="VIN32_07540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEY8922464.1"
                     /translation="MIDEGPTMSLIEHLEELRRRLIIVVIAILAAAVIGFIFSGSVLD
                     LLRAPLPSAYDTIYFTGPADAFGVQVKIAGFIGIALAMPVILFHVWRFVTPGLTKRER
                     RFVWPVMVAAILLFALGIIVGYAIIPYALNFLLGFARPGLQPLLTIDGYVGFVTTMLL
                     AFGIVLEFPIVLVGLARVGILSYRRLAAQRRLAVVGITVFAIVITPGGDPISPIILGS
                     VMYLLFEGSLLVIRLIRR"
     gene            1489..>1999
                     /locus_tag="VIN32_07545"
     CDS             1489..>1999
                     /locus_tag="VIN32_07545"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HEY8922465.1"
                     /translation="MTGTARVLLFSDPAMAGHHPPGHPERPERVEAVISGIADAAASS
                     GASLERPRVESADPDALERIHPAPYLALLDDAAEQGGGWIDGDTFIGPDSMVAARLAA
                     GATLQAALAAVRGEAEVAFAAVRPPGHHARRERAAGFCLLNNVAVAAMALRAAGLAQR
                     IAILDWDVHH"
BASE COUNT          273 a          719 c          682 g          325 t
ORIGIN      
        1 acctggggga ggacagcgtt gagccgttcc tcgcctatcc cgaccgactc gtctacctcg
       61 tcgcgcgcac caccaatccg agcgcgggga cgctccagga cctgccggtg ggtggcactc
      121 cgctgcacga gcacgttgca acgtgggcgg ccgggagatg gaccgatgga cgggtcgggc
      181 tggtcgtcgg ggccaccgcc ccagccgagc tggcgcgcct gcggcgcctg gtgccggcgc
      241 tcggcttcct ggtccccggc gtcggtgccc aggacggcga cctggccgcg gccttggaga
      301 attgccatgg cacggcggcc cccgggctgg tcagcatctc gcgcggcatc gcgggggcgt
      361 cgcagggcga cggatggcgc gccgcggcgg ccgcatcggc ccagtcgtgg ctcggccgaa
      421 tgcaggaggc cggtgctaca ctgccggcct agcgccctgc caaccggcgc gcgccgcgcc
      481 gccaatgagg aatcggtaag cacatgggac ccatcggttg gcctgagctc atcatcctgc
      541 tcgtggtcgt gctgatcgtc ttcggcccgg gcaagctgcc tgacatcggc aatgccatcg
      601 gccgcggcgt gaaggaattc cgcaaggcgt cgaacgacct cgagagctcc attcgcggtg
      661 agccgagcaa gcccgcgccg ccgccgcccg cgaccacgga cgacaagcca caggcctgac
      721 ccacccccgg gtctgagcga tgggggaggc gacgacctgc acatgaggca tccgtgatcg
      781 acgaaggccc gacgatgagc ctcatcgagc atctcgagga gctgcgccgg cgactgatca
      841 tcgtcgtcat cgccatcctc gccgccgccg tcatcggctt catcttctcc gggtcggtgc
      901 tcgacctgct ccgggctccc ctgccgagcg cctacgacac gatctacttc accggtccgg
      961 cggatgcctt tggggtccag gtcaagatcg ccggattcat cggcattgcg ctggccatgc
     1021 cggtgatcct gttccacgtc tggcgcttcg tgacgcccgg gctgaccaaa cgagagcgcc
     1081 gcttcgtctg gccggtgatg gtggccgcga tccttctgtt cgcgctggga atcatcgtgg
     1141 gctacgcgat catcccgtac gcgctgaact tcctgctcgg cttcgcccgg ccgggtctgc
     1201 agccgctgct gacgatcgat ggctatgtgg gcttcgtcac caccatgctg ctggccttcg
     1261 ggatcgtcct cgagttcccg atcgtcctgg ttggcctcgc gcgggtcggc atcctgagct
     1321 atcggcgcct ggccgcccag cggcgcctgg ccgtcgtggg gatcacggtc ttcgccatcg
     1381 tcatcacccc cggcggagac ccgatctcgc cgatcatcct cggcagcgtg atgtacctgc
     1441 tcttcgaagg gtcgctcctg gtcatccgct tgatccggcg ctagaccgat gaccggcacg
     1501 gcgcgtgtgc tgctcttcag cgaccccgcc atggccggcc accatccacc gggccacccc
     1561 gagcgaccgg agcgcgtgga agccgtcatc tccggcatcg ccgatgcggc cgcgtcgagc
     1621 ggagcaagcc tcgagcggcc gcgcgtcgaa tccgccgacc cagacgcgct ggagcggatc
     1681 catcccgccc cgtacctcgc gctgctcgat gacgcggccg agcagggcgg cggctggatc
     1741 gacggcgaca cgttcatcgg tccggactcg atggtggccg cgcgactggc ggccggagcc
     1801 acgctccagg cagctcttgc cgcagtcagg ggagaggcag aggtggcctt cgcggcggtc
     1861 cgtccgccgg ggcaccacgc tcgccgcgag cgcgccgcgg gcttctgcct gttgaacaac
     1921 gtggcggtgg cggcaatggc gcttcgcgcc gccggcctgg cccagcggat cgcgatcctc
     1981 gactgggacg tgcaccacg
//
LOCUS       DATCQR010000225         7285 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_121424, whole genome shotgun sequence.
ACCESSION   DATCQR010000225 DATCQR010000000
VERSION     DATCQR010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 7285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7285
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121424"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /locus_tag="VIN32_07550"
     CDS             complement(<1..350)
                     /locus_tag="VIN32_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015801881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY8922466.1"
                     /translation="MTLETTPSAAARMTAPHIVTELPGPRAQALIARDHAVVSPSLTR
                     AYPLVAETGDGYVVTDVDGNRFLDFAAGIAVCSTGHRHPKVVEAIKAQADRLIHIAAT
                     DFYEPRYLELTEHLA"
     gene            complement(429..1481)
                     /locus_tag="VIN32_07555"
     CDS             complement(429..1481)
                     /locus_tag="VIN32_07555"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013588552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HEY8922467.1"
                     /translation="MPLRRIAYRAIRPLLFRFDSEEIHRFSLRALRMAGASRPGRAMA
                     GLAGGASLEGAEAEVAGLRFRSRVGLGAGFDKDAVALRGWAALGLGFAEIGTVTPLPQ
                     RGNPRPRLFRLPADGALINRMGFNNAGAKAVASNLAAARRRLPQDFVVGVNIGRNAKT
                     TADDTIRDYLAVQQRLAPHADYFAVNVSSPNTPGLRDLQTPKHLRALLDALADAGQRL
                     GPARPIFVKLAPDMLPGEMEAVLEAVLATRAKGLILANTSLSRDRLAAPGELASEAGG
                     LSGAPLLTGTRGLVRRARSLVGDRLAIIASGGIGSGQDAATLIAAGADLVQLWSGLVY
                     AGPGLIGEATNAVRPA"
     gene            complement(1481..1876)
                     /locus_tag="VIN32_07560"
     CDS             complement(1481..1876)
                     /locus_tag="VIN32_07560"
                     /inference="COORDINATES: protein motif:HMM:NF025484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4288 domain-containing protein"
                     /protein_id="HEY8922468.1"
                     /translation="MTTEELPLEPVVDPSGWFSVQLRYAYLVDGGPIMVEDRVVLIDG
                     VDEETALGRAIEVAEEYEDEFETDTGDSGVIRFEGIVALKELDDPPAAGAEVWHEFMG
                     APGIEPEADEEPLPDLRPVEMAFPRAHDD"
     gene            complement(1873..3468)
                     /locus_tag="VIN32_07565"
     CDS             complement(1873..3468)
                     /locus_tag="VIN32_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY8922469.1"
                     /translation="MATTTPRMKITYATLSADNEELQSAFDAAVERVRGELGRSYPML
                     IGAEQRNGAEEFEDRSPIDSSIVVSRFPIGTRQDVRDAIAAARAAFPAWRDLGWRKRL
                     ELMRRAADLISERQFEYAALMSFEVGKTRLEALGDVEETADLLRYYSDGMEKANGFVQ
                     PMGSLSPAEHTRSILKPHGVWAVISPFNFPMALCGGPAAGALIAGNTVVIKPSSDAPL
                     MAYKFCEALRDAGVEPGVFNLVTGPGETVGAELEENPDLDGMVFTGSYEVGMRLYTGF
                     TKDYPRPIITEMGGKNPSIVTASADLDEAAEGVMRSAFGYDGQKCSANSRVYVERSVA
                     RPFLNLLVEKVKEIRVGDPTRRESWMGPVINERALGKFTAAVEEAKADGGKIEVGGEV
                     LRDDATERGYFPMPTVVSGLPTSHRLFRDELFVPFIVVGEVDSLDEALNLSNATAYGL
                     TAGIFSHDDEQIRSFLDTIQAGVVYVNRRAGATTGAWPGIQSFGGWKGSGSSGKSGLG
                     PYYVQQFLREQSQTVMGQEPPPA"
     gene            complement(3613..4308)
                     /locus_tag="VIN32_07570"
     CDS             complement(3613..4308)
                     /locus_tag="VIN32_07570"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY8922470.1"
                     /translation="MTRAILPAWTDLDPALHARLGEVIDPDRKVLAALERIVPLAGKR
                     IADVGTGIGHYPMLLAKRTGRTYGIESDPVLLDVARQRASSAHQPNLRIVEGGLEALP
                     LRDEAVDIVLCGLIEPNDASLPGIAELLRVLRPGGRLVVIGYYGRDQVAGLLEPEVVA
                     HALDATQRRTGWWLRHGFKIKVVHSRLDISDPVLAMELLPRLYGDRGRAFLMAPHPPF
                     LTLKLGLFHLLKR"
     gene            complement(4305..4928)
                     /locus_tag="VIN32_07575"
     CDS             complement(4305..4928)
                     /locus_tag="VIN32_07575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8922471.1"
                     /translation="MRLASVAAALMLASCSLQTSPGPSARSSTGTSAGPSPTESIRPS
                     ATPQPSPSDSGRLTLPAPTGTDPNPITATIGVEVAAAASGQLVITVTNLGKEMVQELV
                     LRWPTAVRDTIFLAPFEPSAQRIREGGDPLVQDWTKWVDGPGERGEPAGTTSVGWGPL
                     LVGGTLTIPLLATRVTPGPITFDLQILSGEKVLQIGGAPAWLQVTVP"
     gene            5117..5509
                     /locus_tag="VIN32_07580"
     CDS             5117..5509
                     /locus_tag="VIN32_07580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8922472.1"
                     /translation="MPIGVRLWFIWAFVLLAGTGILLPRIIEFVDFSERAPFSLLGLF
                     MMLELAALLFGITVAMQRKRIGHRFAIGIAILAAPMLAGLPPILLDFPPEAVAGWQAL
                     FIPIGLLISVVLVFMLLRPGSRAYFSED"
     gene            5538..6593
                     /gene="tdh"
                     /locus_tag="VIN32_07585"
     CDS             5538..6593
                     /gene="tdh"
                     /locus_tag="VIN32_07585"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009708713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HEY8922473.1"
                     /translation="MTTAAAPPRTMRALAKTRPEPGLELVQRPIPVPGPGEVLLKMEA
                     ASICGTDLHLFRWDPWASEIVKPPTILGHELAGRVVAMGAGVTRVAEGDLVGVESHIV
                     DGTCAQCRRGDRHLCRNLKVIGAHVDGGFAEFVVIPEANAIESNGLDPAVVALQEPMG
                     NAVHAAFVEPIEGRTVLVTGCGPIGLCAVGIAKAAGAALVVATDTEEYRLELARGMGA
                     DLALDAGDPGTVDRIRAATDGDGVEVVLEMSGAQPALDQALAAITRGGRISLLGIFPE
                     PVRVDLSDFVIQKGLRIYGIYGRRIYDTWERTQALLRSGALDVTPIITHRFDLADWEQ
                     AFDLIASRHAGKVVLLP"
     gene            6590..>7285
                     /locus_tag="VIN32_07590"
     CDS             6590..>7285
                     /locus_tag="VIN32_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY8922474.1"
                     /translation="MTDTVSARSRNPLAFLDAEVEDLKAKGLYRRLRVVQSEQRSRCV
                     IDGREVITLSSNNYLGLNTHPRLREAAIEAVRRFGAGSGAVRTIAGTMSLHEELERRL
                     AEFKQVPAVLTFQSGFTANTGVIPIVAPEGAVIVSDALNHASIIDGIRLTKAERKVFA
                     HADIDALRAVLTEVRAAPGGADRTVLVITDGVFSMDGDIARLPEIVDAAEEADAIVYV
                     DDAHASGVLGRNGR"
BASE COUNT         1076 a         2520 c         2560 g         1129 t
ORIGIN      
        1 gccaggtgct cggtcagctc gaggtagcgc ggctcgtaga agtcggtcgc ggcgatgtgg
       61 atgagccgat ccgcctgggc cttgatcgcc tcgaccacct tggggtgccg atggccggtc
      121 gagcagacgg cgatgccggc cgcaaagtcg aggaagcgat tgccgtcgac atcggtgacg
      181 acgtacccgt cgccggtctc ggcgaccagc ggatacgcgc gcgtcaggct gggactgacg
      241 acggcgtggt cccgtgcgat gagcgcctgg gcacgcggtc ccggcagctc ggtgacgatg
      301 tgcggcgccg tcatgcgggc agccgccgag ggagtggtct cgagggtcat cggtaccaaa
      361 actccgaccg gagcggcgcg tcctgcccgc gcccctggct agatgggtcc gtatcgtatc
      421 agcccgcctc aggcgggccg taccgcattc gtcgcctcgc cgatcagtcc cggcccagcg
      481 tagaccagcc cgctccacag ctggaccagg tcggcaccgg cggcgatgag cgttgcggcg
      541 tcctggccgg agccgatgcc acccgaggcg atgatcgcca accgatctcc gaccaggctg
      601 cgggctcggc gcacgagccc ccgggtcccc gtcaggagcg gggcgccgga gaggccgccc
      661 gcctccgaag cgagctcgcc gggtgccgcc aggcgatccc tggagaggga cgtgttggcg
      721 aggatcagcc ccttcgcgcg agtcgcgagc acggcttcca ggaccgcctc catctcaccg
      781 gggagcatgt ccggcgcgag cttgacgaag atcggccgcg cagggccgag ccgctggccg
      841 gcgtccgcca gcgcgtcgag cagcgcccgc aggtgcttcg gggtctggag gtcgcgcagg
      901 cccggcgtgt tgggggagga cacattgacc gcaaagtagt cggcatgggg tgcgagccgt
      961 tgctggacgg cgaggtagtc gcgaatcgtg tcgtcggcgg tggtcttcgc gttgcgcccg
     1021 atgttgaccc ccaccacgaa gtcctgcggc aaccgtcgac gggccgctgc caggttgctg
     1081 gcaaccgcct tcgcgccggc gttgttgaag cccatccggt tgatgagcgc cccgtcggcg
     1141 ggaagccgaa agaggcgcgg ccggggattc ccgcgctgtg ggagcggcgt gacggtgccg
     1201 atctcggcga agccgaggcc gagggcggcc cagccacgca acgcgacggc gtccttgtcg
     1261 aagccggcgc ccaggccgac gcggctgcgg aagcgaagcc cggcgacctc ggcctcggct
     1321 ccctcgagcg aggcgccacc cgccaggccg gccatcgctc gcccgggtct gctggcgccg
     1381 gccatgcgca gcgcccgcag gctgaagcgg tggatctcct cagaatcgaa ccggaagagg
     1441 agcggacgga tcgcgcgata ggcgatgcgc cgcaagggca tcagtcgtcg tgcgcgcgcg
     1501 gaaatgccat ctcgaccgga cgcaggtccg ggagcggttc ctcatcggct tcgggctcga
     1561 tccctggcgc ccccatgaac tcgtgccaga cctccgcgcc cgcggctggc gggtcgtcga
     1621 gctccttgag cgccacgatc ccctcgaagc ggatcacgcc agagtcgccg gtatcggtct
     1681 cgaactcgtc ctcgtattcc tcggcgacct cgatggcacg ccccagggcc gtctcctcgt
     1741 cgaccccgtc gatcagcacg acgcggtcct ccaccatgat cggccccccg tcgacgagat
     1801 aggcgtaccg gagctggacg ctgaaccagc cacttggatc gacgaccggc tccagcggga
     1861 gctcctcggt cgtcatgccg ggggcggctc ctggcccatc acggtctgcg actgctcgcg
     1921 caggaactgc tgcacgtagt agggacccag cccgctcttg cccgatgagc ccgagccctt
     1981 ccagccgccg aatgactgga tccccggcca ggccccagtc gtcgccccgg cgcgacggtt
     2041 gacgtacacc acgcccgcct ggatcgtgtc caggaagctg cggatctgct cgtcgtcgtg
     2101 gctgaagatt ccagccgtca gcccgtaggc ggtcgcgttc gagaggttca gcgcctcgtc
     2161 cagcgagtcg acctcgccga ccacgatgaa cgggacgaac agctcgtcgc ggaagaggcg
     2221 gtggctggtc ggcaggccgc tgaccacggt tggcatcggg aagtagccgc gctcggttgc
     2281 atcgtcgcgc aggacctcgc cacccacctc gatcttgccg ccatcggcct tggcctcctc
     2341 cacggctgcc gtgaacttgc cgagcgcccg ctcgttgatg acggggccca tccagctctc
     2401 gcgccgggtc ggatcgccga cccggatctc cttcaccttc tcgaccagca ggttgaggaa
     2461 cggccgcgcc accgagcgtt ccacgtagac ccggctgttg gcgctgcact tctggccgtc
     2521 gtagccgaat gccgagcgca tcacgccctc cgccgcctcg tcgaggtcgg ccgaggcggt
     2581 cacgatcgac gggttcttgc cccccatctc ggtgatgatc ggcctcgggt agtccttggt
     2641 gaagccggtg tagagccgca tgccgacctc gtagctgccg gtgaagacca tgccgtcgag
     2701 gtccgggttc tcctccagct cagcgccgac cgtctcaccc gggccggtca ccaggttgaa
     2761 gacgcctggc tcgacgccgg catcgcgcag cgcctcgcag aacttgtagg ccatcagcgg
     2821 tgcgtccgag ctcggcttga tgacgacggt gttgcccgcg ataagggctc ccgcggcggg
     2881 ccctccacac agcgccatcg ggaagttgaa cgggctgatc accgcccaga cgccgtgcgg
     2941 cttcaggatc gagcgggtgt gctcggccgg ggagagcgag cccatcggct gcacgaatcc
     3001 gttcgccttc tccatcccgt cgctgtagta gcgcagcagg tcggccgtct cctcgacgtc
     3061 gccgagcgcc tcgagccggg tcttgccgac ctcgaacgac atgagagcgg cgtattcgaa
     3121 ctgccgctcg ctgatcaggt cggcggcgcg acgcatgagc tccaggcgct tccgccagcc
     3181 gaggtctcgc caggcgggga aggctgcgcg tgccgcggcg atggcgtcgc gcacgtcctg
     3241 ccgcgtgcct atcgggaagc gcgagacgac gatgctcgag tcgatcggcg agcggtcctc
     3301 gaactcctcg gctccgtttc gctgctccgc gccgatgagc atcgggtacg agcggccgag
     3361 ctcaccgcgg acacgctcca cggcggcgtc gaaggcggac tgcagctcct cgttgtccgc
     3421 ggacagcgtg gcgtaggtga tcttcatccg gggggtcgtg gtggccatct cgcctctcgg
     3481 gcctcggggg gtcgccatta tcgcatccgc ttcacggcga caggaatgcc aaaggccagg
     3541 ctccgggggg agaagagcct ggccttcgac gggtcgaagc atagatgccg ccttgggagg
     3601 ctgcaacgag tatcagcgct tgagaaggtg aaacaggccg agcttcaagg tcaggaaggg
     3661 gggatggggg gccatcagga acgcccgtcc gcggtctccg tagaggcgtg gcaggagctc
     3721 catggcaagg accgggtccg agatgtcgag gcggctgtgg acgaccttga tcttgaagcc
     3781 gtggcgcagc caccagccag tccgtcgctg ggtcgcgtcg agggcgtgcg cgaccacctc
     3841 gggctcgagc aaaccggcga cctggtcgcg gccgtagtag ccgatgacga ccaggcgacc
     3901 ccctgggcgc aacacccgca gcagctcggc gatgccgggc agcgaggcat cgttcggctc
     3961 aatcagcccg caaaggacga tgtcgaccgc ctcgtcccgc aggggcagcg cctcgagccc
     4021 accctccacg atgcgcaggt tgggctgatg cgccgagctg gcccgctggc gcgcaacgtc
     4081 cagcaggaca ggatccgatt cgatgccata tgttcggcca gtgcgcttcg ccagcagcat
     4141 cgggtagtga ccgattcccg tccccacgtc cgcgatccgc ttcccggcca gcggcacgat
     4201 ccgctcgagc gcggccagca ccttgcggtc cgggtcgatg acctctccca accgggcatg
     4261 cagggcggga tcgagatcgg tccatgccgg caggatggcg cgggtcacgg cacggtcacc
     4321 tgcagccagg ccggtgcgcc accgatctgc aagaccttct cgccgctcag gatctggagg
     4381 tcgaaggtga tcggcccggg ggtgacccgc gtcgccagca gcgggatggt gagggtgcct
     4441 ccgaccagga gcggtcccca gcccaccgag gtcgtgcccg ccggctcgcc gcgctcgccc
     4501 ggcccgtcca cccatttcgt ccaatcctgc acgagggggt cgccgccctc gcggatgcgc
     4561 tgcgccgatg gctcgaacgg tgccaggaag atcgtgtcgc gaaccgcggt cggccagcgc
     4621 aggaccagct cctgcaccat ctccttgccg aggttggtga ccgtgatgac cagctgcccc
     4681 gaagctgcgg ccgcgacctc caccccgatc gtagcggtga tcgggttcgg atcggttccg
     4741 gtcggcgccg gcagggtcag ccgcccgctg tcgctcgggc tcggctgtgg ggtcgcgctc
     4801 ggtcttattg attccgtcgg cgaagggccc gcggaggtgc ccgtggacga tctcgcgctt
     4861 gggccgggcg aggtctgcag cgagcaggag gccagcatca gggcggcggc caccgaagcg
     4921 aggcgcatcg gccaggaagc ggattggaga cggatcggtt gtgcgacgct cacggtggcc
     4981 tcgcctgcgg actcatcggc gcgtggccgg cggccaggca ctggaatggt atgcgagccg
     5041 cacctccacc gctgctagga tcgatgggat gtccgacacg tccgaacccg atcaggagcc
     5101 cggctcgcct ccgccgatgc ccatcggcgt ccggctctgg ttcatctggg ccttcgtgct
     5161 cctggcgggc accggcatcc tgctgcctcg catcatcgag ttcgtcgact tcagcgagcg
     5221 ggcgcccttc agcctgctcg gcctcttcat gatgctggag ctggccgcgc tcctgttcgg
     5281 aatcacggtg gcgatgcagc gcaagcggat cgggcatcgt ttcgcgatcg ggatcgccat
     5341 cctcgccgcc ccgatgctgg ccggcctgcc gccgatcctg ctcgactttc cgccggaggc
     5401 ggtcgcgggc tggcaggcgc tcttcatccc gatcgggctg ctcatcagcg ttgtgctcgt
     5461 cttcatgctg ctacggccgg gttcgcgggc ctacttcagc gaggattgag gcgcggggcg
     5521 ctccctatac tggctccgtg accaccgccg cagcgccgcc gcgcacgatg cgtgcgctcg
     5581 ccaagacgcg acccgagcct ggcctggagc tggtccagcg gcccatcccg gtgccagggc
     5641 ccggcgaggt gctgctgaag atggaggcgg cctcgatctg cggcactgac ctgcacctct
     5701 tccgctggga tccctgggcc agcgagatcg tcaagccgcc gaccatcctg gggcacgagc
     5761 ttgccggccg cgtggtggcg atgggagcgg gcgtgacccg ggtcgctgag ggggacctgg
     5821 tcggggtcga gagccacatc gtggacggca cctgcgcgca gtgccgtcgc ggcgatcggc
     5881 acctgtgccg caacctcaag gtgatcggcg cgcacgtgga cggcggattc gcagagttcg
     5941 tggtgattcc cgaggcgaac gcgatcgagt cgaacggtct cgaccctgcg gtggtggctc
     6001 tccaggagcc gatgggcaac gccgtgcacg ccgccttcgt cgagccgatc gagggacgga
     6061 cggtgctggt caccggctgc ggaccgatcg gcctctgcgc ggtcgggatc gccaaagccg
     6121 ctggggcagc cttggtggtc gccaccgaca cggaggagta ccggctcgag ctggcacgcg
     6181 gcatgggcgc cgacctggcg ctcgacgcgg gcgacccggg caccgttgac cgcatccgcg
     6241 ccgccaccga cggcgatggg gtcgaggtgg tgctcgagat gagcggggcg cagcctgcgc
     6301 tcgaccaggc cctggcggcg atcacccgtg gcgggcgaat cagcctgctc ggcatcttcc
     6361 ccgagcctgt tcgcgtcgac ctgtccgact ttgtgatcca gaaggggctg cgcatctacg
     6421 gcatctacgg ccgccgcatc tacgacacgt gggagcggac ccaggcgctg ctgcgctccg
     6481 gtgcgctgga cgtcacgccg atcatcaccc atcgcttcga cctcgccgac tgggagcagg
     6541 ccttcgacct gatcgcttcc cgacacgccg gaaaggtggt gctcctgcca tgaccgatac
     6601 cgtcagcgcg cgctcccgca acccgcttgc gttcctcgac gcagaggtcg aggacctgaa
     6661 ggccaagggc ctgtaccggc gcctgcgggt cgtgcagagc gagcagcgca gccgctgcgt
     6721 gatcgacggg cgcgaggtga tcacgctgtc gagcaacaac tacctcggcc tgaacacgca
     6781 cccccgcctg cgggaggctg cgatcgaggc ggtccgacgc tttggggcgg ggtcgggagc
     6841 cgtccgcacc atcgcgggca cgatgagcct ccacgaggag ctggagcgcc gcctggccga
     6901 gttcaagcag gtgcccgccg tgctgacctt ccagtcgggc ttcacagcca ataccggcgt
     6961 gatcccgatc gtggcgccgg agggtgcggt catcgtctcc gacgcgctga accacgccag
     7021 catcatcgac ggcatccggc tcaccaaggc cgagcgcaag gtctttgccc acgccgacat
     7081 cgacgcgctg agggcggtgc tgaccgaggt ccgcgcggcg ccgggaggcg cggaccggac
     7141 cgttctcgtc atcaccgatg gcgtcttcag catggacggc gacatcgcgc gactgcccga
     7201 gatcgtcgac gccgccgagg aggccgatgc gatcgtctac gtcgacgacg cgcatgcctc
     7261 cggcgtgctg ggccggaacg ggcgg
//
LOCUS       DATCQR010000226         2801 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_228855, whole genome shotgun sequence.
ACCESSION   DATCQR010000226 DATCQR010000000
VERSION     DATCQR010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2801
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228855"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /locus_tag="VIN32_07595"
     CDS             complement(<1..410)
                     /locus_tag="VIN32_07595"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEY8922475.1"
                     /translation="MRLAVLSDIHANLAALDAVCDELGSFDELWVLGDIVGYGPQPNE
                     VIRRLQELGARAVTGNHDGAAIGTVDASWFNPDAQAAIEWTTQVLDENARAYLGSLPE
                     VRRDGDLTAVHGSPREPIWEYITDAAIATANLAA"
     gene            complement(433..1191)
                     /gene="rsmG"
                     /locus_tag="VIN32_07600"
     CDS             complement(433..1191)
                     /gene="rsmG"
                     /locus_tag="VIN32_07600"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF014575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEY8922476.1"
                     /translation="MPSTDRGEAAQARSALADLLADVPAIADILPQGFMDAAERFVDL
                     LLAANRRVNLTRVVAPAEVARLHLLDSLAALPEIDRMGPAEAIDLGSGGGLPGLPLAM
                     ARPAMRWTLVDSVGKKATILEEFVAALRLHNVTVIADRAETVGRSAGQRERYDLVTAR
                     ACAALPVLTELAMPLLVVGGSLLAWKGPLSEADEEVRRGQAAAAQLGAGALRIVDPGV
                     AALGGHRLVVVPKERATEARFPRRPGQPGRRPLG"
     gene            1456..1848
                     /locus_tag="VIN32_07605"
     CDS             1456..1848
                     /locus_tag="VIN32_07605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8922477.1"
                     /translation="MAATLTEGSPRFAHQSEAELARILDFYGVAWRYEPDLFPISWNA
                     DGVVVESFAPDFYLPEIDIYLELTTLKQTLVRKKNRKLRWLRQLYPEVRVKLFYARDF
                     RALMVKYGRLDFMADVAAMNGNGPLNAR"
     gene            1848..2399
                     /gene="hpt"
                     /locus_tag="VIN32_07610"
     CDS             1848..2399
                     /gene="hpt"
                     /locus_tag="VIN32_07610"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEY8922478.1"
                     /translation="MHADVEEILLSGEEVQARVAELGAQLAADYESRDPVLVSVLKGS
                     IIFLADLVRAMPIPLSIDLMEVSSYGASTESSGQVRILKDLSTSIEGREVIVVEDIID
                     TGLTLNYLLRYLHDKGPASIRICCLLDKPARRLAPIEIDYRGFTIADRFVIGYGLDYG
                     ERYRNLPYIGVLRPSVYGAAPPA"
     gene            2406..>2801
                     /locus_tag="VIN32_07615"
     CDS             2406..>2801
                     /locus_tag="VIN32_07615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8922479.1"
                     /translation="MPDAGPGGRYRVPILLGSLLMVAAGFLTWWQAGGESIEGVPIPS
                     SAGIGLEGPGIVVYGAALATLVLLDIGYMRGRWGFVLDGPWVYLALGLVAAGALAYRI
                     WELWSVGFLPLPQRSPGLAAAAVGIGLVLY"
BASE COUNT          418 a          988 c          952 g          443 t
ORIGIN      
        1 aacgcggcga ggttggccgt ggcgatggcc gcgtcggtga tgtactccca gatcggctcg
       61 cgtggcgatc catgcactgc cgtcaggtcg ccgtcgcgac gcacctccgg cagcgagccc
      121 aggtaggcac gcgcgttctc atcgagcacc tgcgttgtcc attcgattgc agcttgggcg
      181 tcggggttga accacgaagc gtcgaccgtg ccgatcgcgg cgccatcgtg gttgcccgtg
      241 actgcgcgcg ccccgagctc ctgcaggcgg cggatcacct cgtttggctg cggcccgtag
      301 cccacgatgt cgccgagcac ccagagctca tcgaacgagc cgagctcgtc gcacacggcg
      361 tcgagggcgg caagattggc gtggatgtcc gacaggacag cgaggcgcat ggggctcgga
      421 gtataccgag ggtcaaccaa gcggccggcg gccaggctgc cccggtcggc gggggaagcg
      481 agcctccgtg gcacgctcct tcggcacgac cacgagccga tgtccgccga gtgcggccac
      541 ccctggatct acgatccgga gcgcgccagc tccaagctgc gcagcggccg cctggccgcg
      601 ccgcacctcc tcgtccgcct cggaaagcgg gcccttccac gccagcagcg agcccccaac
      661 gaccagcagt ggcatggcca gctcggtcag caccggcagg gcggcgcagg cccgggcggt
      721 gacgaggtcg tagcgctccc gttgcccggc ggatcggccg accgtctccg cgcggtccgc
      781 gatgaccgtg acgttgtgca ggcggagtgc cgcgacgaac tcctccagga tggttgcctt
      841 cttgccgacc gagtcgacca gcgtccagcg catcgccggc ctcgccatgg ccagcggcag
      901 ccccgggagc ccgccgcccg agccgaggtc aatcgcctcg gccggcccca tccggtcgat
      961 ctcgggcagg gcagcgaggg agtcgagcag gtgcaggcgc gcaacctccg cgggtgccac
     1021 gacccgggtc aggttgactc ggcggttggc cgcgagcagc aagtccacga accgctcagc
     1081 ggcatccatg aagccttgcg gcaggatgtc ggcgatggcc ggcacatcgg cgagcaggtc
     1141 cgcgagggcg ctgcgcgcct gggctgcctc gccgcgatca gtgctcggca aaggaaaatg
     1201 cctctccggt ggcgtttcgg tcttcccggt cacgagcggt tggtcggccg gctttcgcat
     1261 cggccccgcg catgcccggt taccgtactc ggccgccggg gaagcaggac ttctaccgta
     1321 ccagcccgcg ccatgccgga caaccggagt tatccaccaa ccaccgcagt cgaagccgtt
     1381 ctgtggataa gtcgccgggc tatactcgcc ctcgatgacg ccaggggccc ggcgggccgg
     1441 tacgatcggg gaccagtggc ggcgacgctg accgaaggct cgccgcgctt cgctcaccag
     1501 agcgaagcgg aattggccag gatcctcgac ttctacggtg tcgcatggcg atacgaaccc
     1561 gacctcttcc ccatctcatg gaacgccgac ggcgtggtcg tcgagtcctt cgcgccggac
     1621 ttctacctgc ctgagatcga catctacctc gagctgacga ctctcaagca gaccctcgtg
     1681 cgcaagaaga accgcaagct gcgctggctg cgccagctct accccgaggt ccgcgtgaag
     1741 ctgttctacg ctcgcgactt ccgtgcgctg atggtcaagt acggtcggct tgacttcatg
     1801 gcggatgtgg ccgccatgaa cggcaacggc ccgctgaacg cccgatgatg cacgccgacg
     1861 tcgaggagat cctgctgagc ggcgaggagg tccaggctcg ggtggcagag ctcggggcgc
     1921 agctggcggc ggactacgag agccgcgacc ccgtcctggt gagcgtcctg aagggctcga
     1981 tcatctttct ggcagacctc gtccgcgcca tgccgatccc gctctcgatc gacctcatgg
     2041 aggtgagcag ctacggcgcc tccaccgagt cgagcggcca ggtgcgcatc ctgaaggacc
     2101 tctcgacctc gatcgagggc cgcgaggtga tcgtggtcga ggacatcatc gacaccggcc
     2161 tgaccctgaa ctacctgctg cgctacctgc acgacaaggg tccggcatcc atccgcatct
     2221 gctgcctgct cgacaagccc gcgcgccgcc tggcaccgat cgagatcgac taccgcggct
     2281 tcacgatcgc cgatcgcttc gtgatcggct atggcctgga ctacggcgag cggtaccgca
     2341 acctccccta catcggggtc ctgcgcccat cggtctacgg cgccgccccg cccgcgtagc
     2401 ccgcgatgcc cgacgcaggc ccaggcggcc gctaccgcgt cccgatcctg ctcggcagcc
     2461 tcctgatggt ggccgccggg ttcctgacct ggtggcaggc cggcggcgag agcatcgagg
     2521 gcgtcccgat cccctccagc gccgggatcg gcctcgaagg cccggggatc gtggtctatg
     2581 gggccgcgct ggcgacgctg gtgctgctcg acatcggcta catgcgcggc cgctggggct
     2641 tcgtcctcga cggtccgtgg gtctacctgg cgcttgggct ggtggcggcg ggggcgctcg
     2701 catatcggat ctgggagctg tggtcggtgg gcttcctgcc cctgccacag cgctctcccg
     2761 ggctcgccgc tgccgcggtc gggatcggcc tggtcctgta c
//
LOCUS       DATCQR010000227         5456 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_90556, whole genome shotgun sequence.
ACCESSION   DATCQR010000227 DATCQR010000000
VERSION     DATCQR010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5456
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90556"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1670
                     /gene="sdhA"
                     /locus_tag="VIN32_07620"
     CDS             <1..1670
                     /gene="sdhA"
                     /locus_tag="VIN32_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HEY8922480.1"
                     /translation="PEARIGVISKLYPSRSHTGAAQGGVCAALGNTEEDNWEWHWFDT
                     VKGGDYLVDQDAAEVMCHDAVETVINLEHLGLPFNRTPEGLIDQRRFGGHTRNYGEGP
                     VRRACYAADRTGHHILQTLYEQCIRHDVLFYDEYQVLDLAMPAGADGPVNGVIALQLS
                     SGEVHSFRAKAVLFATGGFGKMFKVTSNAHTLTGDGVAIAYRRGLPVEDMEFYQFHPT
                     GMYKLGFLLSEAMRGEGGFLVNGKGERFMERYAPTMKDLASRDVVSRSIYQEIAAGRG
                     VDGKDFVHLDVRHLGAKVLNEKLPDMSGFIRTYFGIDPLTELVPIQPTAHYAMGGIPT
                     DIDGRVLGDETGRVVPGFFAAGECACVSVHGANRLGTNSLLDILVFGRRAGIAMAEEV
                     RRAPLPEPDPAAEASVTAGLEELLARTDGERPAAIREELQETMFAKCGVYRSATLLMQ
                     AWGEVDDLRSRARQLVVEDKGKRYNTDLGEALELLFLLDCAEATVASAVARTESRGAH
                     AREDYPDRDDVDWLKHTFAFRWSDGAPPRLAYRPATITRFQPKPRTY"
     gene            1674..2375
                     /locus_tag="VIN32_07625"
     CDS             1674..2375
                     /locus_tag="VIN32_07625"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HEY8922481.1"
                     /translation="MDVQLRILRFDPERDDLPHWEEYAVPAEPSDRVLDLLHQVKWYQ
                     DGTLTFRRSCAHGVCGSDAMLINGRNRLACEYLVKDGGREISVEPVPGSRVVKDLLID
                     LDGFWEKYRSVMPYLVNDEVPEDGKERRQSPEEREQFEDTSKCILCGACTTACPSFWA
                     NPDYVGPAAIVNAHRFIFDSRDHATEERLEIMADADGVWRCRTIFNCVEACPRGINIT
                     RAIMEVTRQIETRRQ"
     gene            2372..2797
                     /locus_tag="VIN32_07630"
     CDS             2372..2797
                     /locus_tag="VIN32_07630"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF024848.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HEY8922482.1"
                     /translation="MSDGASPRRVLIYTDGAARGNPGPAGLGAILRDAETGKVLAEMA
                     RFLGVRTNNYAEWTAVEDALREALLMGADHVDLRMDSELVARQITGRYRVKHPDLKPI
                     HARVMAMLGQLKGYTVGHVPRELNKDADHLSNVAIDERR"
     gene            2811..3126
                     /gene="rnpB"
                     /locus_tag="VIN32_07635"
     ncRNA           2811..3126
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VIN32_07635"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(3244..3480)
                     /locus_tag="VIN32_07640"
     CDS             complement(3244..3480)
                     /locus_tag="VIN32_07640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8922483.1"
                     /translation="MAVEKLDSGTRIPIPRQGRIRRCRVRHVSILPAGDGPRVQVDCL
                     LGGLEHPLPLGSMDEARSICQACTATTIWRADED"
     gene            3676..4599
                     /locus_tag="VIN32_07645"
     CDS             3676..4599
                     /locus_tag="VIN32_07645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8922484.1"
                     /translation="MFGRRDRSRESREAAEREQSRRALFEELAKRPANICPFLGLASS
                     QTEYHDGFTREHRCYAFGDPVELSAEQQERVCLGRGYGNCPRYLRGVLVIPTEELEAL
                     RRPLPPVRPPAPPDTAPPPPPEPDGSGRRPVFLLLGLVLLLAVVGAGGAFFYFNGQGV
                     AVRPTPTPQPSVSSQPSVTIVPTPTQEPSPTPEPTPFPETPKPDPTPLPGDTFQGYEV
                     TVLEGENTLFEVDDAGKIVSSQVAHYSRFSKAPVDRIVAGNGLLHWRTSEGFFTGLSY
                     IRDRSGAFLIRAVYQGADGEKRYIVLKQEDI"
     gene            4735..5181
                     /gene="mraZ"
                     /locus_tag="VIN32_07650"
     CDS             4735..5181
                     /gene="mraZ"
                     /locus_tag="VIN32_07650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEY8922485.1"
                     /translation="MQSSFLTGEFRHTLDDRSRVAVPVRFRQRLAQGATLARWLDNCL
                     GLFPADAWDELAGKISALPLTNPRAREFARFMSSGAVEVELDKQGRILVPGYLREYAG
                     LSDGEVVVVGALNRLEIWAPAAWLPYRSKIEDEPEALAEHLADLGI"
     gene            5273..>5456
                     /gene="mraW"
                     /locus_tag="VIN32_07655"
     CDS             5273..>5456
                     /gene="mraW"
                     /locus_tag="VIN32_07655"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014435080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytosine(1402)-N(4))-methyltransferase"
                     /protein_id="HEY8922486.1"
                     /translation="MGEGHLPVLVDEVLASLAVGPGSSIADCTVGGGGHAERLLEASS
                     PDGRLLGLDADRAAILE"
BASE COUNT          904 a         1835 c         1892 g          825 t
ORIGIN      
        1 ggccggaggc ccgcatcggc gtcatcagca agctgtatcc ctcccgctcg cacaccgggg
       61 ccgcgcaggg gggcgtgtgc gccgcgctcg gcaacaccga ggaggacaac tgggagtggc
      121 actggttcga cacggtcaag ggaggcgact acctggtcga ccaggacgcc gccgaggtga
      181 tgtgccacga cgcggtcgag acggtcatca acctggagca cctgggcctg ccgttcaacc
      241 gcacccctga gggactgatc gaccagcgcc ggtttggcgg ccacacccgc aactacggag
      301 agggacccgt gcgccgagcg tgctatgccg ccgaccgcac cgggcaccac atcctgcaga
      361 ccctctacga gcagtgcatc cggcacgacg tcctcttcta tgacgagtac caggtgctcg
      421 acctggccat gccggccggg gcggacggac cggtcaacgg ggtcatcgcc ctccagctct
      481 cgagcggcga ggtccactcg ttccgggcga aggcggtgct cttcgcgacc ggcggcttcg
      541 gcaagatgtt caaggtgacg agcaacgcgc acacgctgac cggggacggg gttgcgatcg
      601 cctaccggcg cggcctgccg gtcgaggaca tggagttcta ccagttccac ccgaccggga
      661 tgtacaagct cggcttcctg ctctccgagg cgatgcgtgg cgaaggtggc ttcctggtca
      721 acggcaaggg ggagcgcttc atggagcgct acgctcccac catgaaggac ctggccagcc
      781 gtgacgtggt gagccgcagc atctaccagg agatcgccgc cgggcgcggg gtcgatggca
      841 aggacttcgt ccacctcgac gtccgccacc tcggcgccaa ggtgctgaac gagaagctgc
      901 cggacatgag cgggttcatc cgaacgtact tcggcatcga cccgctgact gagctggtgc
      961 cgatccagcc gacggcgcac tacgccatgg gcgggatccc caccgacatc gatgggcggg
     1021 tcctgggcga cgagacgggc cgcgtggtgc ccggcttctt tgcagcgggg gagtgcgcct
     1081 gcgtgagcgt gcacggcgcg aaccggcttg ggaccaactc gctgctcgac atcctcgtgt
     1141 tcgggcgccg ggcggggatc gccatggccg aggaggtgcg ccgggcgccg ctgccagagc
     1201 ccgatcccgc cgccgaggca tcggtcacgg ctgggctcga ggagctcctg gcccgcactg
     1261 atggtgagcg cccggcggcg attcgcgagg agctgcagga gacgatgttc gcgaagtgcg
     1321 gcgtgtatcg atccgcgacc ctgctgatgc aggcgtgggg cgaggtagac gatctccgca
     1381 gccgtgcgcg gcagctcgtg gtcgaggaca aggggaagcg gtacaacacg gacctgggcg
     1441 aagccctcga gctcctcttc ctgctcgact gcgcggaggc gaccgtggcg tcggccgtcg
     1501 cccgcaccga gagtcgaggc gcgcacgcgc gcgaggacta ccccgatcga gacgatgtcg
     1561 actggctgaa gcacaccttc gccttccgct ggtccgacgg agcgccaccg cggctcgcct
     1621 accggccggc cacgatcacc cgcttccaac cgaagccgcg aacctactga ccgatggacg
     1681 tccagctgcg gatccttcgc ttcgaccccg agcgcgatga cctcccgcac tgggaggagt
     1741 acgccgtgcc ggccgagccc tcggaccggg tgctcgacct gctgcaccag gtgaagtggt
     1801 accaggacgg gacgctcacc ttccggcgct cgtgcgcgca tggcgtctgc ggctccgacg
     1861 cgatgctgat caacggccgg aatcggctgg catgtgaata cctcgtcaag gacggggggc
     1921 gcgagatcag cgtcgagccg gtgccgggct cgcgggtcgt caaggacctg ctgatcgacc
     1981 tcgacgggtt ctgggagaag taccgctcgg tgatgccgta tctcgtcaac gacgaggtgc
     2041 cggaggatgg caaggaacgg cgccagtcgc ccgaagagcg ggagcagttc gaggacacga
     2101 gcaagtgcat cctgtgcggc gcctgcacga ccgcgtgtcc atccttctgg gccaaccccg
     2161 actacgttgg tccggcggcg atcgtcaacg cccatcggtt catcttcgac agccgcgacc
     2221 atgcgaccga ggagcggctg gagatcatgg ccgacgccga cggggtgtgg cgctgccgga
     2281 cgatcttcaa ctgtgtcgag gcgtgccccc ggggcatcaa catcacccgc gcgatcatgg
     2341 aggtcacccg ccagatcgag acgcggcgtc agtgagtgac ggggcctccc ctcgacgagt
     2401 cctgatctac accgatggcg ctgcccgtgg caatcccggt ccggccggtc tgggtgcgat
     2461 cctgcgcgat gccgagacgg gcaaggtcct tgccgagatg gcgcgcttcc tgggggtacg
     2521 caccaacaac tatgccgagt ggactgcggt cgaggatgcg ctgcgtgagg cgctcctgat
     2581 gggcgccgat catgtcgacc tgcggatgga ctcggagctg gtggcgcggc agatcaccgg
     2641 ccgctaccgg gtcaagcacc ccgacctcaa gccgatccat gcgcgcgtga tggcgatgct
     2701 cggccaactc aagggctaca cggtcggcca cgtcccgcgt gagctcaaca aggatgccga
     2761 tcatctctcg aacgtggcaa tcgacgagcg gcgatagaat gctcgtccct ggaggcggcc
     2821 ggacggccgc gggcgcgatc gtcgaaaggc ggcgctcgag gaaagtccga gctccataga
     2881 gcaggttcgc gggtaacgcc cgtccgccgt gaggcgagga ccagcgccac agtgacgatg
     2941 cccgcgcaag cgggagtgaa acgcggcaag ctcgagccgg agcaagaccg aataggaggg
     3001 cgcaaggcgg cgttcgcgcc gcagcaggct gctcgctcag ccctcgggta ggtcgctcag
     3061 atggatggcc gtcgcggcgc cgcagggcgc cggcacagaa ctcggcttac aggccgctct
     3121 ccagggacat aacggatgca gagagcgcag ccctgattgg ggtgagggtg gacgggctgg
     3181 gccaggcgcg aggatgttgc gaagccggcc gagggcctgg cccagtgcgg ccgacccatc
     3241 ggcctagtcc tcgtctgccc gccagatcgt cgtcgccgtg caggcctggc agatggatcg
     3301 cgcctcgtcc atggaaccca gcgggagtgg atgctccaga ccgccgagca ggcagtcgac
     3361 ctggacgcgc ggcccatcgc cggcaggcag gatcgaaacg tggcgcacgc ggcaacgacg
     3421 gatgcgtccc tgccggggga tcgggatccg cgtgccggaa tcgagctttt cgaccgccat
     3481 gcttcgacct tcccctcgtt ggccggggtg gcgttgaacc ggccgtcccg cagcctatgt
     3541 tccctgcacc gtggagctca atggggcgtt ggtaccatcc agacccctcc tgcaacgcgc
     3601 tgggcgtttc ggccgcgcta cactcgatcg ggggttgggt cgcgcagcgc tgaacgagac
     3661 gaggtcgcca aggcattgtt cggtcgtcga gatagaagcc gggagagccg tgaggcggcc
     3721 gagcgtgagc agagccggcg ggctctcttc gaggagcttg ccaagcggcc cgccaacatc
     3781 tgcccgttcc tgggcctggc cagctcccag accgaatatc acgatgggtt cacccgcgag
     3841 caccgctgct acgcgttcgg tgacccggtc gagctctcgg ccgagcaaca ggaacgcgtc
     3901 tgcctggggc gcggctatgg gaattgcccc cgctacctgc gcggcgtact ggtgatcccg
     3961 accgaggagc tggaggcgct gcgtcgcccg ctgccccccg tccgtccgcc ggcgcccccc
     4021 gacacggcgc ctcctccgcc accggagcca gacgggagcg gccggcgacc cgtcttcctg
     4081 ctgctcggac tggtgctcct gctggcagta gtcggggccg gcggggcctt cttctacttc
     4141 aacggccagg gagtggccgt gcgaccgacc ccgacccctc agccatcggt gagcagccag
     4201 ccgagcgtca ccatcgtgcc gacaccaacg caggagccct cgccgactcc ggagccgaca
     4261 ccattcccgg agacccccaa gcccgatccg acccccttgc ccggcgacac gttccaggga
     4321 tacgaggtca ccgtgctgga gggagagaac acgctcttcg aggtcgatga cgccggcaag
     4381 atcgtgagct cacaggtcgc ccactacagc cgattctcca aggctccggt ggaccgcatc
     4441 gtggcgggga acggcctcct ccactggcgg accagcgagg gcttcttcac cggcctctcc
     4501 tacatccgcg accggtcggg cgcgttcctc attcgcgccg tgtaccaggg cgccgacggg
     4561 gagaagcgct acatcgtcct caagcaagag gacatctaga accggaccgt ctcccaggac
     4621 catcgtccgg tgcaattcat ggcagaaatc ccttgacggc gtactgctgc cctgtacaat
     4681 ggcgcccggt ggcccaaaat ggggaccaat ggggagtagt ggtagcaggc tcgcatgcag
     4741 tcctcgttcc tgacaggtga attccggcac acgctcgacg accgcagtcg cgtcgccgtc
     4801 cccgtgcgct ttcggcagcg cctggcacag ggggccaccc tcgctcgctg gctcgacaac
     4861 tgcctgggcc tctttccagc cgacgcgtgg gatgagctcg ccgggaagat ctcggcgctg
     4921 ccgctgacca atccgcgcgc tcgggagttc gcgcgcttca tgtcgtcggg tgcggtggag
     4981 gtcgagctcg acaagcaggg tcgaatcctg gtgccggggt acctgcgcga gtacgccggc
     5041 ctttccgacg gagaagtggt cgtggtgggg gcgctcaacc ggctcgagat ctgggcaccg
     5101 gcggcctggc tgccgtatcg ctcgaagatc gaagacgagc ccgaggcgct cgccgaacac
     5161 ctggccgatc tcgggatctg aggacgcgac gaatgacgca cgccgcgaac cctccattca
     5221 tgcgtctccg ggccgatcgg cccgccgtcc gacacgaaag gtgcgaccgc gaatgggaga
     5281 agggcacctg ccggtcctcg tcgacgaggt tcttgcctcg ctggcggtgg gtcccggcag
     5341 ctccatcgca gactgcacgg tcggcggcgg tgggcacgcc gagcggctct tggaggcctc
     5401 gtccccggat ggccgcctgc tcggcctcga cgccgatcgg gcggccatcc tggaga
//
LOCUS       DATCQR010000228         2632 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_59187, whole genome shotgun sequence.
ACCESSION   DATCQR010000228 DATCQR010000000
VERSION     DATCQR010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2632
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59187"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /locus_tag="VIN32_07660"
     CDS             complement(<1..243)
                     /locus_tag="VIN32_07660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8922487.1"
                     /translation="MSAAPGSAPLASAVASRLSRWADERVAERLWSRDGSLWAASGKS
                     PAEVASWLGWLDLPVAMQERIGEFERIAREVRDDGYT"
     gene            complement(240..1343)
                     /gene="tal"
                     /locus_tag="VIN32_07665"
     CDS             complement(240..1343)
                     /gene="tal"
                     /locus_tag="VIN32_07665"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HEY8922488.1"
                     /translation="MNALQRLHAEQDQSPWIDFIDRQLIDSGKLAEWVGMGIRGLTSN
                     PTIFAKAVASGQYDELIRREIAAGHDAREIFEEIEIADCGDAADILRPVYEESDGADG
                     FVSIEVEPDLAEDSDATLERARHLWGRMARPNVFIKIPATPAGLPAIESAIAEGINVN
                     ITLMFSVDVYRDVARAYITGLQRRRDAGGEIARVASVASFFVSRVDTKVDKALDAIGT
                     QAAIEARGQAAIANAKLAYHAYKEIFEGGAFDDLAAAGAREQRCLWASTSTKNPTYRD
                     VMYADELIGPKTVDTLPTETIEAFLDHGVVSRTLDVGLDEALAAIRAVESLGIRMETV
                     TDELIVEGVASFTKSFEDLMGVIDAQRKALAPA"
     gene            complement(1343..>2632)
                     /locus_tag="VIN32_07670"
     CDS             complement(1343..>2632)
                     /locus_tag="VIN32_07670"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011873997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEY8922489.1"
                     /translation="MHGWDLDLFAARARAFGWHAIEIDGHDLGAIDGAFAEATGIEDR
                     PTVVLARTEKGHGVAAVANKNGFHGKPLADPDAAIAELGGIRNLSVAVARPSGPADPH
                     QFAASGDLQLPAYEVGSKEATRKAYGDALVALGAVRGDVVALDGEVGNSTYSEEFAAA
                     YPERFIQCYIAEQQMVAIAVGLQVRGWRPFAATFAAFLARAYDFVRMAAVSQADIVLV
                     GSHAGVSIGEDGPSQMALEDIASLRAVFGSTVLYPSDPNQTAALLAEIVDRKGIVFLR
                     TTREKLPVIYPAGEAFEIGGSRVVRRTDADRVTLIGAGITLHEAISAAALLADEGIAA
                     RVIDCYSVKPIDETTLRTAAAETGGIVTVEDHWPEGGLGDAVLEVFAADSVRPRIVKL
                     AVRHMPGSATPAEQLAMAGIDAAHIADAARELVQEIR"
BASE COUNT          392 a          942 c          878 g          420 t
ORIGIN      
        1 ggtgtacccg tcgtcccgaa cctcgcgggc gatccgctcg aactccccga ttcgctcctg
       61 catcgccacc ggaagatcca gccagccgag ccacgaggcg acctcggccg gcgacttgcc
      121 ggatgccgcc cacaggcttc cgtcgcgcga ccacagacgc tcggcgaccc gctcatccgc
      181 ccagcgggag aggcgtgaag ccaccgcgga cgccagcggg gcggagcctg gagccgcgct
      241 cacgcgggag cgagcgcctt gcgctgcgcg tcgatgacgc ccatcaggtc ctcgaatgac
      301 ttcgtgaagg aagccacccc ctccacgatc agctcgtcgg tgacggtctc catccggatc
      361 ccgagcgact ccaccgcgcg aatcgcggcg agcgcctcat ccagccccac gtccagcgtg
      421 cggctcacga ccccgtggtc gaggaacgcc tcgatcgtct cggtcgggag cgtgtcgacc
      481 gtcttgggac cgatcagctc atcggcatac atgacgtcgc gataggtggg gttcttggtc
      541 gaggtgctcg cccacaggca gcgctgctct cgggcccccg cagccgccag gtcgtcgaac
      601 gctcccccct cgaagatctc cttgtaggcg tgataggcga gcttcgcatt ggcgatggcc
      661 gcctggccgc gcgcctcgat ggcggcctgc gtgccgatcg cgtcgagcgc cttgtcgacc
      721 ttggtgtcca cacggctcac gaagaacgag gccaccgagg ccactcttgc gatctcgcct
      781 cccgcatcgc gccgacgctg gagccccgtg atgtaggcgc gcgccacgtc ccggtagacg
      841 tccacgctga acatcagcgt gatgttgacg ttgatcccct cggcgatcgc cgactcgatg
      901 gccggcaggc ccgccggcgt ggctggaatc ttgatgaaga cgttggggcg cgccatgcgg
      961 ccccacaggt gccgcgcccg ttccagcgtt gcgtccgaat cctcggccag gtcgggctcg
     1021 acctcgatcg agacgaagcc atcggcgccg tcggactcct cgtagacggg ccgcaggatg
     1081 tcggccgcgt cgccgcagtc ggcgatctcg atctcctcga agatctcgcg cgcgtcgtgg
     1141 ccggcggcga tctcgcggcg aatcagctcg tcgtactgac cgctggcgac ggccttggcg
     1201 aagatggtcg ggttgctggt gaggccgcgg atccccatcc ccacccattc ggccagcttc
     1261 ccgctgtcga tcagctggcg gtcgatgaag tcgatccatg ggctctggtc ctgctcggcg
     1321 tgcagccgtt gcagcgcgtt catcatcgga tctcctgtac gagttcacgg gcggcgtctg
     1381 caatatgggc ggcgtcgatc ccggccatgg cgagctgctc ggccggcgtc gccgagccgg
     1441 gcatgtgacg aaccgccagc ttgacgatcc gtggacggac ggaatcggcg gcgaagacct
     1501 cgagcacggc gtcgccgagc ccgccctccg gccagtggtc ctcgacggtg acgatgccgc
     1561 cggtctccgc agcagctgtg cgcagggtgg tctcgtcgat cggcttcacc gaatagcagt
     1621 cgatcacccg agcggcaatc ccctcgtcgg caaggagcgc tgccgccgag atggcctcgt
     1681 gcagcgtgat ccctgccccg atcagggtga cccggtcggc atcggtccgg cgcacgaccc
     1741 ggctgccccc gatctcgaac gcctcgccgg cgggatagat gaccggcagc ttctcgcgcg
     1801 tggtgcgcag gaagacgatc cccttgcgat cgacgatctc tgccagcagc gcggcggtct
     1861 ggttgggatc cgatggatac aggaccgtgc tcccgaacac ggcgcgcaac gaggcaatgt
     1921 cctcgagcgc catctgcgag ggaccgtcct cgccgatgct gactccggca tgcgagccga
     1981 ccagcacgat atcggcctga ctgacggctg ccatccgcac gaagtcgtag gcgcgtgcca
     2041 ggaacgcggc gaaggtcgcg gcgaagggtc gccagccgcg cacctggagt ccgacggcga
     2101 tggccaccat ctgctgctcg gcgatatagc actggatgaa gcgctccggg tacgccgccg
     2161 caaactcctc ggagtaggtc gagttgccca cctcgccatc cagggcgacg acatctccgc
     2221 gcaccgctcc gagcgccacg agggcgtccc cgtacgcctt gcgggtcgcc tccttgctgc
     2281 cgacctcgta ggcgggcagc tgcaggtcgc cgctagccgc gaattggtgc gggtcggccg
     2341 gtccgctggg ccgggcgacg gccacgctca ggttgcggat gccgcccagc tcggcgatgg
     2401 cggcgtcggg gtccgcgagg ggcttgccgt ggaatccatt cttgttggcg accgcggcca
     2461 ccccatgccc cttctcggtg cgcgccagca cgacggtcgg ccgatcctcg atcccggtcg
     2521 cctcggcaaa cgccccgtcg atggcgccca ggtcgtggcc gtcgatctcg atggcatgcc
     2581 agccgaaggc gcgcgcccgc gcggcgaaca gatccaggtc ccagccatgc at
//
LOCUS       DATCQR010000229        10758 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_28276, whole genome shotgun sequence.
ACCESSION   DATCQR010000229 DATCQR010000000
VERSION     DATCQR010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 10758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10758
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28276"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1149)
                     /locus_tag="VIN32_07675"
     CDS             complement(<1..1149)
                     /locus_tag="VIN32_07675"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEY8922490.1"
                     /translation="MAHTPPPANGHVRELHPTGIFAAVGRFDYRFRRWLPIVGLAIVI
                     GTNAWAATSGGTLIQGGWVVAGSEEQRAAALIADRFGSEATTMLVVYRAPDGDAASPE
                     FQATVKASLARVADDPAVTKIQRYGDTGAPALLSNDGTMTMAAVYLDKGVEEAVNDAG
                     RLADEVERPAGVEMWVTGIPQVYHEFNAKIETDLFQAEAISLPIALLILLAVFGTLVG
                     AALPLVIAGFAMPTAFAAISLLANVTEMSIFVTNIATMIGLALAIDYSLFMVSRFREE
                     LRHHDVEIALERMMGSVGKAVAVSGIAVAVGLSSLVVFDAAALRSMGLGGILTVLSTL
                     IFGLTVLPALLALLGPRVNRLRVPLPRFLSLIEDDAAAADRRQGHGVWA"
     gene            1275..1496
                     /locus_tag="VIN32_07680"
     CDS             1275..1496
                     /locus_tag="VIN32_07680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8922491.1"
                     /translation="MAWLGPLAYGAIIGLLQPSLGRDAYRIAILSLLVLMVVGFLIVR
                     RVPEGRESDGESPLASPLEPAIVLPGEAP"
     gene            1493..2251
                     /locus_tag="VIN32_07685"
     CDS             1493..2251
                     /locus_tag="VIN32_07685"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HEY8922492.1"
                     /translation="MTLVVAHRGAATVAPENTMEAYRRAVEMGADAIKLDVQLTADGE
                     LALMHDETVERTTDLTGPIATMTLKQLRAADAGFRFEAPDGTFPFRGKGLTVPTLREV
                     LDWLPDGTGLVVEAKARAATAPTIEALRGSRVRAAGAVSLISFEEAIIDEARGLDPEI
                     PTGYLLVPSQPIEAALTYAVEHGHAAVHPYEGDLGLDPGPILSLATAYGRLMGCYVVN
                     DPARMQQLAAYGLWGFVTDVPEVGRQALGARVSQ"
     gene            complement(2268..2645)
                     /locus_tag="VIN32_07690"
     CDS             complement(2268..2645)
                     /locus_tag="VIN32_07690"
                     /inference="COORDINATES: protein motif:HMM:NF013962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEY8922493.1"
                     /translation="MNAFIVELPNRPGSLAMISEAIAERGINISGIAGATSGELGSVA
                     FITDDESATRNALGEKGWVYREVPVVTASLDDQPGTLAAAARRLADAGINVETMFVTG
                     KDGDKVKIAFGVDTPEAAQRALG"
     gene            2738..3634
                     /locus_tag="VIN32_07695"
     CDS             2738..3634
                     /locus_tag="VIN32_07695"
                     /inference="COORDINATES: protein motif:HMM:NF018024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HEY8922494.1"
                     /translation="MTYSIVARDPATGRLGVAVQSHYLGVGPVVPWLEAGVGAIATQA
                     SVNVSFGPMGLEMLRAGQAADAVVAALVAGDEQAQVRQLGVVDSQGRAAAHTGADCIP
                     ACGHLVREGFTVQGNLLERDTCWPAMAAAYEGALAEEAPFVERLLRAMEAAEAEGGDV
                     RGRQSAAIMLASAELQPAAWRGRLLDLRIEDHPDPVPELRRIVTMQLAYNLLDEEGDE
                     AKAGRSTAERYELARQMSPDAYELVFWRGVELATSGDVEAGRRELQVAFAADSRWRTT
                     LQHLADANREGVTPELAEQLLD"
     gene            complement(3651..4655)
                     /gene="trxB"
                     /locus_tag="VIN32_07700"
     CDS             complement(3651..4655)
                     /gene="trxB"
                     /locus_tag="VIN32_07700"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEY8922495.1"
                     /translation="MTATVPPATDSADTSSTDTRHHKLAIIGSGPAGLTAAIYAARAN
                     LEPMVFAGNTPLGQLMITSDVENYPGFPGGIQGPELMQLFRDQAERFGSKLLDKDVTR
                     VDFSQRPHRLWVGDDEYQADAVIVATGASALWLGLESEEQFRGRGVSACATCDGFFFR
                     DREIAVVGGGDTALEEALFLTRFASKVTMLVRREAFRGSKIMQDRAFAHPKVEVRWNS
                     EVTDVRGEMTVNELGLRDTVTGEVSDLPVQGLFIAIGYKPNTDLFAGQLDLDERGYCV
                     VEGGTKTKVEGVFVAGDVHDHLYRQAVTAAGDGCRAAIDAERWLGSVGEIEASTATNW
                     "
     gene            4736..5965
                     /gene="ispG"
                     /locus_tag="VIN32_07705"
                     /gene_synonym="gcpE"
     CDS             4736..5965
                     /gene="ispG"
                     /locus_tag="VIN32_07705"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010887031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEY8922496.1"
                     /translation="MIDRRPTVSVDVSGVLIGSGHPIVVQSMTNTDTADADATAIQVA
                     QLAHAGSELVRVTVNNDAAAEAVPIIRQKLDGLGVGVPLIGDFHYNGHLLLTKYPGCA
                     AALAKYRINPGNVGTKRHDERFRAIVEVAIANGKPVRIGVNWGSLDQQLLTELMDANA
                     ELSEPRESRDVMIDAMLESALRSAQLAEETGLGHDRIIISAKVSGVQDLVEVYTRLAE
                     RCDYPLHLGLTEAGMASRGIISSTAGLAILLQRGIGDTIRVSLTPSPGGDRTEEVQIA
                     QQVLQSLGLRSFTPQVSACPGCGRTTSTFFQEMARDIGAYLRDQMPVWSRTHPGVEEL
                     RVAVMGCVVNGPGESKHADIGISLPGTFEEPVAPVFVDGQLRTTLRGDDIVPRFLEIL
                     EDYVARRYPQALAASPS"
     gene            complement(5980..6189)
                     /locus_tag="VIN32_07710"
     CDS             complement(5980..6189)
                     /locus_tag="VIN32_07710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8922497.1"
                     /translation="MEPNLPATAEETALVLDTTHDLHGDYVDPQVQVVRQNVRVALLP
                     LAIIAFTVIAFIAAAWTFLTALPPA"
     gene            complement(6195..6779)
                     /locus_tag="VIN32_07715"
     CDS             complement(6195..6779)
                     /locus_tag="VIN32_07715"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEY8922498.1"
                     /translation="MTATTGEALRGAAARGGAGRLPRWYYLAAALAPFLLLALWGAPR
                     LVGPAVPVLAQVGGPAPAFELADLDGKPVSLADLRGRPVVVNFWASWCGHCVEEFPLL
                     KSAAATYRDKGLAVVGIVFRDGSGPARDFMTRLGATWPALMDPGEAVASRFGIIWPPD
                     TYFIDRGGMVMSRQIGQLSAADLERGLAGILGEE"
     gene            complement(6787..7134)
                     /locus_tag="VIN32_07720"
     CDS             complement(6787..7134)
                     /locus_tag="VIN32_07720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8922499.1"
                     /translation="MTDVAFGYLLLAIGITFVLGVVYAITSHVSVSTARPRPPRGVHL
                     PNPSALPVILAVGGALLGAGLAFRADNQLANPFLAIPGLAIFLYAVVAWVRAAGREWD
                     EVERGPHDDAAGH"
     gene            complement(7147..7770)
                     /locus_tag="VIN32_07725"
     CDS             complement(7147..7770)
                     /locus_tag="VIN32_07725"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HEY8922500.1"
                     /translation="MTVQVPTSVSADITTHTDLSHREAGMPTPLVGMLLFIASEVMFF
                     GGLFASYFNARAVHIGEWGPPSGAPGLEVFPIALPITIILVASSFTMQLGVWAIRRGD
                     QRAMRNWTLVTLLLGVTFLIGQIYDYTQLGFGISDGAFGTVFYTLTGFHGAHVFGGAV
                     GLTILAARASQGQFSKQNHVAVEAISFYWHFVDVVWIALFTTLYFLR"
     gene            complement(7767..9419)
                     /gene="ctaD"
                     /locus_tag="VIN32_07730"
     CDS             complement(7767..9419)
                     /gene="ctaD"
                     /locus_tag="VIN32_07730"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005517859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HEY8922501.1"
                     /translation="MATTALTPTAESYRRSWVIEWLTTVDHKKIGILYIVNSFAFFFA
                     AGILALLVRSELAVPGLQFLDERTYNQAFTMHGTIMIFLFVVPVLSGFANYIVPLQLG
                     APDMAFPRINALSFWFLPLGGLLIFSGYLFGGAAAEGWTLYTPLSGGKYSTGVGTDLW
                     LIGLLLVGTASILGSVNFIATIFKMRAPGMTLFRMPILVWTVLVTATLILLATPVLAA
                     GMIALFIDRNYGGSFFDPNTGGNPILWQHLFWFFGHPEVYIVILPAMGVVSEILPVFT
                     RRPLFGYRAFVFATIAIGLLSFSVWAHHMFTTGQVFLPFFSFMTALIAIPTGVKMFNW
                     LATLWRGSIVFATPMLFALGFLSMFLIGGISGVMLSAPAVDFHLQDTYFVVAHLHYVF
                     FGGSVFGVFAATYYWFPKMTGRRLNEPLGKVHFWMHFVGFNIAFFPQHILGIQGMPRR
                     IADYSPDAGWTILNLVSTAGAFLIAASILPFLVNVVATFINGEKVGDDPWEGNTLEWA
                     TSSPPPPYNFDKLPPIRSERPVFDLRHRHDAEHVSVAKVEDQ"
     gene            complement(9441..10529)
                     /gene="coxB"
                     /locus_tag="VIN32_07735"
     CDS             complement(9441..10529)
                     /gene="coxB"
                     /locus_tag="VIN32_07735"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEY8922502.1"
                     /translation="MRSLETELMSRPMARPRIRALLRVGALLAPVLLATACGMVPPEP
                     HTTQAKQVFWLYNVILVMGGIVFVAVEGFIVYSVFRYRRRDDRLPTQIHGNNLVELIW
                     TAIPTVIVLILFVLSTATLNSVSQASTHPGVTIEVEGKQWSWVFHYLDDDGNPANDVI
                     VQGTALDGAVMAVPVGEPVKLILISNDVIHSFYVPAFLVKRDVVPLPEGKAPNELEFT
                     VSEKGTYSGQCAEFCGDLHARMTFSVQAMSRADFDKWTTAIRAGETPAPSVPAGGGVL
                     KVSAKNTAFDTKALEAVAGQPFKIEFTNDDSFEHNVGIWNGSDELFRGEIFPGPATKT
                     YDVKGLPAGQYTFVCDVHPAMTGTLTVN"
BASE COUNT         1773 a         3576 c         3780 g         1629 t
ORIGIN      
        1 ggcccacact ccgtgtccct gcctgcggtc ggccgcagcg gcgtcgtcct cgatcaggct
       61 gaggaagcgc ggcagtggca cgcgcagtcg gttgacgcgc gggccgagca gggccagcag
      121 cgcggggagc accgtcagcc cgaagatcag cgtcgaaagc accgtcagga tcccgcccag
      181 ccccatgctg cgcagtgccg cggcgtcgaa gacgacgagc gacgagagcc cgaccgccac
      241 cgcgatgccg gataccgcca ccgccttgcc gacgctgccc atcatccgct cgagcgcgat
      301 ctcgacatcg tggtggcgaa gctcctcgcg gaatcggctc accatgaaga gcgagtagtc
      361 gatcgccagc gccaggccga tcatcgtcgc gatgttcgtg acgaagatgc tcatctcggt
      421 gacgttcgcc agcaggctga tcgccgcgaa ggcggtcggc atcgcgaatc cggcaatcac
      481 gagtgggagg gcagcgccga ccagtgtgcc gaagacggcg agcaggatca gcagcgcgat
      541 cggcaggctg atcgcctccg cctggaagag gtccgtctcg atcttggcgt tgaactcgtg
      601 atagacctgc gggatgccgg tgacccacat ctcgacgccg gcgggtcgct ccacctcgtc
      661 ggccagccgc ccggcgtcgt tgaccgcctc ctcgactccc ttgtcgaggt agaccgccgc
      721 catggtcatg gttccgtcgt tgctcagcag cgccggcgcg ccggtgtcgc cgtacctctg
      781 gatcttcgtc acggcggggt cgtccgccac acgggcaagg ctcgccttga ccgtggcctg
      841 gaactcgggc gacgcggcgt cgccgtcggg ggcgcggtag acgacgagca tggtggtcgc
      901 ctcgctgccg aagcggtcgg cgatcagcgc ggcggcgcgc tgctcctccg agccggcgac
      961 cacccagcca ccctggatca gggtcccgcc cgaggtggcc gcccaggcat tcgtgccgat
     1021 cacgatcgcc aggccgacga tcggcagcca gcgccggaac cgatagtcga agcgcccgac
     1081 cgcggcgaag atgccagtcg gatgcagctc ccgcacgtgc ccgttcgcgg gcggcggggt
     1141 gtgggccacg gacaacgatt cacctcgagt gcgcagggtt cgggattcgc tcgggcgaag
     1201 ggtactgggt cggtatgatc cgcgcgtgat ttgcccagcc cgcgcccggt gaccagcctc
     1261 gccccgaccg cctggtggcg tggcttgggc cgctcgcgta tggggcgatc atcgggctcc
     1321 tccagccctc gcttggtcgt gatgcctacc ggatcgcgat cctgagcctg ctggtgttga
     1381 tggtggttgg ctttttgatc gtgcggagag tccccgaggg tcgggagtcg gatggcgagt
     1441 cgccgctggc ctcgccgctc gagccggcca tcgtgctgcc gggggaggcg ccatgacact
     1501 ggtcgtcgcg caccgtggcg ccgccacggt cgcccccgag aacacgatgg aggcctatcg
     1561 ccgtgcggtc gagatgggtg ccgacgccat caagctggac gtccagctga ccgccgacgg
     1621 tgagcttgcg ctgatgcacg acgagacggt ggagcgcacc accgacctca ccgggccgat
     1681 cgccacgatg acgctcaagc agctgcgcgc ggccgacgcc ggcttccgct tcgaggcgcc
     1741 cgacggcacc ttccccttcc gcggcaaggg cctcaccgtc ccgaccctgc gggaggtgct
     1801 cgactggctg cccgacggca ccgggctggt ggtcgaggcc aaggcgcgcg ccgcgacggc
     1861 tcccacgatc gaggccctcc gcggctcgcg tgtccgcgcc gccggggccg tgagcctgat
     1921 cagcttcgag gaggcgatca tcgacgaggc ccgcgggctc gaccccgaga tcccgaccgg
     1981 ctacctgctg gtcccgtcgc agccgatcga ggcggctctg acctacgccg tggagcacgg
     2041 tcatgcagcg gtccatccgt acgaggggga tctcgggctc gatcctgggc cgatcctctc
     2101 ccttgccacc gcgtacggcc gcctgatggg ctgctatgtc gtgaacgacc cggcccgcat
     2161 gcagcagctt gccgcctacg ggctgtgggg cttcgtcacc gacgttccgg aggtgggcag
     2221 gcaggcactt ggtgcgaggg tcagccagtg attcgggagc gccggcccta gccgagggcg
     2281 cgctgggcag cctcaggcgt gtcgactccg aatgcgatct tcaccttgtc gccgtccttg
     2341 ccggtgacga acatggtctc gacgttgatg cccgcatctg ccaggcggcg tgcagccgca
     2401 gccagcgttc ccggctgatc gtcgaggctg gcggtcacga ccggaacctc gcggtagacc
     2461 catcccttct cgcccagcgc gttcctggtg gcggactcgt cgtcggtgat gaaggcgacc
     2521 gaccccagct cgccggaggt ggcacccgcg atgccgctga tgttgatccc gcgctctgcg
     2581 atcgcctccg agatcatggc caggctcccc ggccggttcg gcagctcgac gatgaatgcg
     2641 ttcatgcgct tctccatgcc gggtcgattc ggaatcggtc gagtgtacag gtggatgtca
     2701 tcatccctgc cttcgcagag gcagctacgc ttcgcccatg acctacagca tcgtcgcccg
     2761 cgaccccgcg accggacgcc tcggcgtcgc cgtccagtcg cactacctgg gcgtcggccc
     2821 ggtggtgcca tggctcgagg ccggcgtggg cgcgatcgcc acgcaggcat cggtcaacgt
     2881 cagctttgga ccgatggggc tcgagatgct gcgcgccggc caggcggccg atgccgtggt
     2941 cgcagcgctg gtggcgggag acgagcaggc gcaggtccgc caactcggcg tcgtcgacag
     3001 ccaggggagg gcggccgcgc ataccggcgc cgactgcatc ccggcctgcg gacacctcgt
     3061 tcgggagggc ttcaccgtgc agggcaacct gctcgagcgc gacacctgct ggccggcgat
     3121 ggctgccgca tacgagggcg cgctggccga agaggcgccc ttcgtcgagc ggctgctgcg
     3181 agcgatggag gcggccgagg cggagggcgg cgacgttcgc ggtcgtcagt cggcggcgat
     3241 catgctcgcc tcggccgagc tgcagcccgc ggcctggcgg gggcggctgt tggacctgcg
     3301 catcgaggat catcccgacc cggtaccgga gctgcgccgg atcgtgacca tgcagctggc
     3361 ctacaacctg ctcgacgaag agggagatga ggcgaaggcc ggccgcagca cagccgagcg
     3421 ctacgagctc gcccggcaga tgtcgccgga cgcctatgag ctcgtcttct ggcgcggagt
     3481 cgagctggcc acgagtggcg acgtggaagc cggccgccgc gagctgcagg tcgccttcgc
     3541 agcagactca cgatggcgca ccacgctgca gcacctcgcc gatgcgaacc gcgagggggt
     3601 cacgccggag ttggcggaac agctgctcga ctagcgtcgg ggtgcggcag tcaccagttg
     3661 gtggcggtcg aggcctcgat ctcgccgacg gaccccagcc agcgctcggc atcgatcgcc
     3721 gcgcggcagc cgtcgcccgc ggcggtgacc gcctggcggt acaggtggtc gtggacgtcc
     3781 cccgccacga agacgccctc gaccttggtc ttcgtgccgc cctcgacgac gcagtagccg
     3841 cgctcatcga ggtcgagctg gccggcgaac aggtcagtgt tcggcttgta gccgatggcg
     3901 atgaagagcc cctggacggg caggtccgag acctcgcccg tgaccgtgtc ccgcaggccg
     3961 agctcgttca ccgtcatctc gccgcggacg tcggtgacct cggagttcca gcgcacctcg
     4021 accttcgggt gtgcgaaggc gcggtcctgc atgatcttgc tgccgcggaa cgcctcgcgc
     4081 cgaaccagca tcgtcacctt cgaggcgaag cgggtcagga agagcgcctc ctcaagggcc
     4141 gtatcgccgc cgcccaccac cgcgatctcc cgatcccgga agaagaagcc gtcacaggtg
     4201 gcgcacgcgc tcaccccccg accgcggaac tgctcctcgg actcgaggcc gagccataga
     4261 gccgaggcgc cggtggcgac gatgacggca tcggcctggt actcgtcgtc gcccacccac
     4321 agccgatgcg gacgctggct gaagtcgacg cgggtcacgt ccttgtccag gagcttgctg
     4381 ccgaagcgct cggcctggtc gcggaagagc tgcatcagct cgggcccctg gatcccgccg
     4441 ggaaagccgg ggtagttctc aacgtcggag gtgatcatca gctggcccag cggcgtgttg
     4501 ccggcgaaga ccatcggctc caggttcgcg cgggcggcat agatggcggc ggtcagtcca
     4561 gcgggcccgg acccgataat cgcgagcttg tggtggcggg tatcggtgct gctggtgtct
     4621 gcgctgtctg tagcgggcgg aacggtcgcg gtcatgaatt cagtctagcg aagtcgtcga
     4681 acggcccgat gtcgccgtcg gggcgtctcc gcctcgatcc ccgtacactc gagccatgat
     4741 cgatcgccgc cccacggtaa gcgtggacgt cagcggggtc ctgatcggct ccggacatcc
     4801 gatcgtggtc cagtcgatga cgaacaccga caccgccgat gcggatgcca ccgccatcca
     4861 ggtcgcccag ctggcccacg ccgggtcgga gctggtgcgt gtcacggtca acaacgacgc
     4921 cgcggcagag gccgtgccga tcatccgcca gaagctggac ggcctgggcg tgggcgtgcc
     4981 cctgatcggg gacttccatt acaacggcca cctgctgctt acgaagtacc ccggctgcgc
     5041 ggcggcgctg gccaagtacc gcatcaaccc gggcaacgtc ggcaccaagc gccacgacga
     5101 gcggttccgt gcgatcgtcg aggtggctat cgccaacggc aagccggtgc gcatcggcgt
     5161 gaactggggc tcgctcgacc agcagctgct gaccgagctg atggacgcca acgccgagct
     5221 gtcggagcca cgcgagtcgc gagacgtgat gatcgacgcc atgctcgagt cggcactccg
     5281 ctcggcgcag ctggccgagg agaccgggct ggggcatgac cgcatcatca tctcggccaa
     5341 ggtctcgggc gtccaggacc tggtcgaggt ctacacccga ctcgccgagc gctgcgacta
     5401 tccgctgcac ctggggctga ccgaggcggg gatggcatcg cgcgggatca tctcctccac
     5461 cgccgggctg gcgatcctcc tgcagcgagg gatcggcgac acgatccggg tgagcctcac
     5521 cccatccccc ggcggcgacc ggaccgagga ggtccagatc gcccagcagg tgctgcaatc
     5581 gctcgggctt cgcagcttca cgccgcaggt cagcgcatgc cctggctgcg gccggaccac
     5641 gtccaccttc ttccaggaga tggcccggga catcggcgcc tacctgcgcg accagatgcc
     5701 cgtctggagc cgcacgcatc ccggcgtgga ggagctgcgc gtggcggtca tgggctgcgt
     5761 cgtgaacggc cctggcgaga gcaagcacgc ggacatcggg atcagcctgc cggggacctt
     5821 cgaggagccg gtggcccctg tcttcgtcga cgggcagctg cgaacgacgc tgcgcggcga
     5881 cgacatcgtg ccgcgcttcc tggagatcct ggaggactac gtcgcgcggc gctatccgca
     5941 ggctctcgcc gcctcgccct cctgaggacg gtcggggggt caggccggcg gcagggcggt
     6001 caggaaggtc cacgccgcgg cgatgaacgc gatcaccgtg aaggcgatga tcgcgagggg
     6061 caggagcgcg acgcggacgt tctgacgcac gacctggacc tgcgggtcga cgtagtcacc
     6121 gtgcagatcg tgggtcgtgt cgagcaccag cgcggtctcc tcggccgtgg ccgggaggtt
     6181 tggttccatg accatcactc ctctcccagg atgcctgcca gcccgcgctc caggtcggcg
     6241 gcactgagct ggccgatctg gcgactcatc accatgccgc cgcgatcgat gaagtaggtg
     6301 tccggcggcc agatgatgcc gaatcgtgac gcgaccgcct cgccggggtc catcagggcg
     6361 ggccaggtcg ctcccaggcg tgtcatgaag tcgcgggcgg gccccgagcc atcgcggaag
     6421 acgatcccga ccactgcaag ccccttgtcc cggtacgtgg ccgccgcgga cttcagcagc
     6481 gggaactcct cgacgcagtg gccgcaccag ctggcccaga agttgacgac caccgggcgt
     6541 ccgcgcaggt ctgcaaggct caccggcttc ccgtcgaggt cggccagctc gaacgccggt
     6601 gccggcccgc cgacctgggc gagcacgggg accgccggcc caaccagccg gggtgcgccc
     6661 cacagggcga ggagcaggaa cggggcgagc gccgcggcaa ggtagtacca gcgcggcagg
     6721 cgacccgcgc ccccccgcgc cgccgctccg cggagcgcct caccggtggt ggcggtcatg
     6781 caggtatcag tgccccgccg cgtcgtcgtg cggcccgcgc tcgacctcgt cccattcgcg
     6841 acccgccgct cgaacccagg ccacaaccgc gtacaggaag atggccaggc cgggaatcgc
     6901 caggaacggg ttggcgagct ggttgtcggc gcggaacgcg agtccggccc ccagcagcgc
     6961 gccgcccacc gccaggatga ctggcagggc gctcggattc ggcaggtgca cgccacgagg
     7021 cggcctgggg cgcgcggtcg agacgctgac gtggctggtg atggcgtaca cgacgcccag
     7081 cacgaaggtg atgccgatcg ccaggagcag gtagccgaag gcaacgtcgg tcattcgatc
     7141 agcctcctag cgcaggaagt acagcgtggt gaagagggca atccagacca cgtccacgaa
     7201 atgccagtag aagctgatcg cctcaaccgc cacgtggttc tgcttgctga actgaccctg
     7261 ggatgctcgt gccgcgagga tggtgagccc caccgcacca ccgaagacat gggccccgtg
     7321 gaagccggtg agcgtgtaga agaccgtccc gaaggccccg tcgctgatcc cgaagccgag
     7381 ctgggtgtag tcgtagatct gaccgatcag gaaggtcacg cccaggagca gcgtgaccag
     7441 cgtccagttg cgcatcgcgc gctggtcgcc acgccggatg gcccacaccc cgagctgcat
     7501 cgtgaagctg gacgcgacca ggatgatcgt gatgggcagg gcgatgggga agacctccag
     7561 ccccggtgct cctgacggcg gtccccactc cccgatgtga acggcgcggg cattgaagta
     7621 ggaggcgaag aggccgccga agaacatcac ctcgctggcg atgaagagga gcatcccgac
     7681 cagtggggtc ggcatcccgg cctcgcggtg agagaggtcg gtgtgcgtgg tgatgtccgc
     7741 gctcacgctg gtcgggacct ggacggtcac tggtcttcca ccttcgcgac cgagacatgc
     7801 tcggcgtcgt gccggtggcg caggtcgaag acgggccgct cactgcgaat cggcggcagc
     7861 ttgtcgaagt tgtagggcgg cggcggagag ctggtggccc actcgagcgt gttcccctcc
     7921 catggatcgt cgccgacctt ctcgccgttg atgaaggtgg cgaccacgtt cacgaggaat
     7981 ggcaggatgc tggcggcgat caggaaggcc cccgctgtcg agacgaggtt caggatcgtc
     8041 cagcccgcat cgggcgagta gtcggcgatg cgacgcggca tcccctggat gccgaggatg
     8101 tgctgcggga agaaggcgat gttgaagccg acgaagtgca tccagaagtg gaccttgccc
     8161 aggggctcgt tcaggcgtcg gccggtcatc ttggggaacc agtagtaggt cgccgcgaag
     8221 acgccgaaga cgctcccgcc gaagaagacg tagtgcaggt gggcgaccac gaagtaggtg
     8281 tcctgcaggt ggaagtcgac ggccggggcg gagagcatca cgccgctgat gccgccgatg
     8341 aggaacatcg acaggaagcc gagggcgaag agcatcggcg tggcgaacac gatcgagccg
     8401 cgccacaggg tggccagcca gttgaacatc ttcacccccg tcgggatcgc gatcagcgcg
     8461 gtcatgaagc tgaagaacgg caggaagacc tgcccggtcg tgaacatgtg gtgggcccac
     8521 acgctgaagc tgagaaggcc gatggcgatg gtggcgaaga cgaaggcccg gtagccgaag
     8581 agcgggcgcc gggtgaagac cggcaggatc tcgctcacca cgcccatcgc cggcaggatg
     8641 acgatgtaga cctcgggatg gccgaagaac cagaagaggt gctgccaaag gatcgggttg
     8701 ccgccggtgt tcgggtcgaa gaagctgccg ccgtagttgc gatcgatgaa gagcgcgatc
     8761 atgccggccg ccagcacggg cgtggcgagc aggatcaggg tggcggtgac gaggaccgtc
     8821 cagaccagga tcggcatccg gaagagggtc atccccggcg cccgcatctt gaagatggtg
     8881 gcgatgaagt tgaccgaccc caggatcgag gcggtgccca ccagcagcag cccgatcagc
     8941 cacaggtcgg tcccaacccc ggttgaatac ttcccgccgc tgagcggcgt gtagagcgtc
     9001 cagccctcgg ccgcggcgcc accgaacagg tagccggaga agatcagcag gccgccgagc
     9061 ggcaggaacc agaagctgag ggcgttgatg cgcgggaacg ccatgtccgg cgcgcccagc
     9121 tggagtggca cgatgtagtt cgcgaacccg gagaggaccg gcaccacgaa caggaagatc
     9181 atgatcgtgc cgtgcatagt gaacgcctgg ttgtaggtcc tctcgtccag gaactggagt
     9241 cccggaacgg ccagctctga gcgcaccagc agggccagga tgcccgccgc gaagaagaag
     9301 gcgaacgagt tgacgatgta caggatcccg atcttcttgt ggtcgaccgt cgtcagccac
     9361 tcgatcaccc acgaacggcg gtagctctcg gcggttgggg tcagggccgt ggttgccatt
     9421 ggttcctcgc tcgcctcgcg ctagttgacc gtcagcgtgc cggtcatcgc cgggtggaca
     9481 tcgcagacga aggtgtattg gcctgctgga aggcccttga cgtcataggt cttcgtcgca
     9541 ggccccggga agatctcccc gcggaagagc tcgtcgctcc cgttccagat tcccacattg
     9601 tgctcaaagg aatcgtcgtt ggtgaactcg atcttaaacg gctggcccgc caccgcctcg
     9661 agcgccttcg tgtcgaaggc ggtgttcttg gcgctgacct tcaggacccc gccgccggcc
     9721 gggacggagg gcgctggcgt ctcgccggcg cgaatggcag tggtccactt gtcgaagtca
     9781 gcccgcgaca tggcctgcac gctgaacgtc atgcgcgcat gcagatctcc gcagaactcc
     9841 gcgcactgac cgctgtaggt ccccttctcg ctgaccgtga attcgagctc gttgggcgcc
     9901 ttgccctccg gtagcggcac gacgtcgcgc ttcaccagga atgcaggcac gtagaaggaa
     9961 tggatcacgt cgtttgagat caggatgagc ttcacgggct ccccgacggg aacggccatc
    10021 accgccccgt cgagcgccgt cccctgcacg atcacgtcat tcgccggatt gccgtcgtcg
    10081 tcgaggtaat ggaagaccca tgaccactgc ttgccctcga cctcgatggt gacgccggga
    10141 tgcgtggatg cctgcgagac cgagttgagc gtcgccgtcg atagcacgaa caggatgagc
    10201 acgatcacgg tcgggatggc ggtccagatc agctcgacca ggttgttgcc gtggatctgg
    10261 gtcgggagcc gatcgtcgcg gcggcgatag cggaagacgg aatagacgat gaagccctcg
    10321 accgcgacga acacgatgcc gcccatcacc aggatcacgt tgtaaagcca gaagacctgc
    10381 ttggcctgcg tggtatgcgg ctcggggggg accatgccgc aggcggttgc gaggaggaca
    10441 ggcgcgagca gggctccgac gcgaaggagg gcccgaatgc gcggccgggc catcggtcgt
    10501 gacatcagct ccgtctcgag gctcctcatc ggcgacgagg acgcggcttg tctcgaggct
    10561 ccgcgtccga ctcggtccca tccgggggca cgtccaccgg ggcggcggca cgcgcggcgt
    10621 attgtaccga tgcgtcctgg gcggcgcacc cctcggccgg ttgcgcggct cggagtcggc
    10681 agcggcctag acagccgggc tggggcactc cagtggctcg tccggcagca ggaatcgagc
    10741 cgtcacctgg taccgatg
//
LOCUS       DATCQR010000230         4107 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_121643, whole genome shotgun sequence.
ACCESSION   DATCQR010000230 DATCQR010000000
VERSION     DATCQR010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4107
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121643"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1253)
                     /gene="radA"
                     /locus_tag="VIN32_07740"
     CDS             complement(<1..1253)
                     /gene="radA"
                     /locus_tag="VIN32_07740"
                     /inference="COORDINATES: protein motif:HMM:TIGR00416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEY8922503.1"
                     /translation="MPATRSVYVCQQCGASSPRWSGQCASCEAWNTLVETVVSRAASG
                     GAGSARARERAASAGSAAISPIGGVSSAEASRLPSGIGELDRVLGGGLVPGSIVLFGG
                     DPGIGKSTLLLQLAGRLAAAGHATLYLTGEESASQVRGRAERVGAVVDGVGLVASTDL
                     ATAVGAIEASAPALAVVDSVQTFASAELGGPAGSVGQVREVATRLAEVAKAGTTCVAL
                     IGHVTKEGTVAGPRTLEHLVDAVIYLEGERMGTTRLLRAAKNRYGSTDEVGVMEMRSD
                     GLVEVADASRFFLESEQPPVAGSAITIALEGTRPLAVEIQALVAPAGYGSPRRATTGI
                     DVNRVLMLIAVLARHAGLNLTGHDVYVNVAGGLRIDEPAADLAVATALASSLRERPVR
                     EGTVLAGELSLSGRLRPAARAERRMA"
     gene            complement(1304..3781)
                     /locus_tag="VIN32_07745"
     CDS             complement(1304..3781)
                     /locus_tag="VIN32_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEY8922504.1"
                     /translation="MDRFDKFTDRARKVLTLAQDEAQRFNHNYIGTEHLLLGLVREGE
                     GVAARVLENMNVELSKVRTAVEFIIGRGDRPVVGEVGLTPRAKRVIELAIDEARRLGH
                     NYIGTEHLLLGLVREGEGIAAGVLESLGVNLDKVRHQVIHVLSQSTSVSPAQETKRPS
                     KTPTLDQLGINLTEAARSGKLDPVIGREKEIERVIQILSRRTKNNPALIGEPGVGKTA
                     IAEGLAHRIVSGDVPETLADKRVLTLDIGSLVAGTKYRGEFEERLKKIIEELRSSSDS
                     VLFIDELHTLVGAGAAEGAIDAANILKPPLSRGELQCIGATTLDEYRKYIEKDAALER
                     RFQPVMVEEPTVDEAVAILFGIRSRYEEHHHVKISDEAVRAAVDLSVRYVADRALPDK
                     AIDLMDEAGSRVRLRESKAPPKIKEAQKDLEDVTRQKDEAIAAQDYEAAARLRDEEAQ
                     GKEAIEALRTAWREEAAKDVPVVTEEEIAQVVSMWTGIPVIRISAEESERLLHMEDAI
                     HKRVIGQQEAIDIISKAVRRARAGLKDPKRPIGSFIFMGPTGVGKTELARALAEFMFG
                     SEDALVKIDMSEFMERHNVSRLVGAPPGYVGYDEGGQLTEAVRRKSYSVVLLDEIEKA
                     HPEVFNILLQILEDGHLTDAKGRRVDFRNTIIIMTSNVGAQLLQKDSRIGFRAEGDAG
                     SVAEATADYERMKEKVLGELKNVFRPEFLNRIDATVVFRELTRAEIREIVDLLLERVK
                     SQLAGQQMELVVTDAAKDAIIVKGYDLSFGARPLRREIQHQIEDPLAERMLQAAFEAG
                     DTIVVDATPDGLVIERKTEKRQPVKAR"
BASE COUNT          577 a         1459 c         1365 g          706 t
ORIGIN      
        1 gccatgcgac gctcggcacg ggcggcgggc cgcagccgtc ccgagagcga cagctcaccg
       61 gccaggacgg tcccttcccg cactgggcgc tcgcgcagcg acgaggcgag cgcggttgcc
      121 accgccaggt ccgcggccgg ctcgtcgatg cgcagcccgc cggcgacgtt cacgtacacg
      181 tcgtggccgg tgaggttcag cccggcgtgc cgggccagca ccgcgatcag catgagcacc
      241 cggttcacgt cgatcccggt cgtcgcgcgg cgtggcgaac cgtagccggc gggcgctacc
      301 agtgcctgga tctcgacggc cagcgggcgg gtcccctcca gggcgatggt gatggcgctg
      361 cctgcgacag gcggctgttc ggattccagg aagaagcggc tggcatcggc aacctcgacc
      421 aggccgtcgc tgcgcatctc catgacaccg acctcatcgg tcgagccgta tcggttcttt
      481 gccgcccgaa ggagccgggt cgtgcccatc cgctccccct ccaggtagat gaccgcgtcc
      541 accaggtgtt ccagggttcg cggacccgcg accgtcccct ccttggtgac gtgaccgatc
      601 agtgccacgc aggtggtgcc agccttcgcc acctccgcca ggcgcgtggc gacctcgcgg
      661 acctggccca cgctgccggc cgggccgccc agctctgcac tggcgaaggt ctggacgctg
      721 tcgaccacgg cgagcgccgg ggccgaggct tcgatcgcac ccaccgcggt cgccaggtcg
      781 gtgctggcga ccagcccgac gccgtcgacc accgccccga ctcgctcggc tcgaccacga
      841 acctgggagg ccgattcctc gcccgtcaga tagagggtgg catgacctgc ggccgccagc
      901 cgccccgcca actgcaggag caacgtgctc ttgccgatcc ccggatcacc gccgaagagg
      961 acgatcgacc ccggaaccaa tcccccgccc agcacgcggt cgagctcacc aatgccgctc
     1021 ggcaggcgcg aggcctcggc actgctgacg cccccgatgg gcgagatcgc tgccgaaccg
     1081 gcagacgccg cccgctcgcg cgcccgcgcg gaacctgccc ccccgctcgc ggctcgactc
     1141 acgaccgttt cgaccagggt gttccacgcc tcgcacgagg cgcattggcc ggaccagcgc
     1201 ggcgagctgg cgccgcattg ctggcagaca tagacggagc gggtagctgg cacgggtcgc
     1261 gccatcttgc cacgccccca cgtactgcgg ggttatcggg cggtcagcgc gccttgacgg
     1321 gctgccgctt ctccgtcttg cgctcgatca ccaggccgtc gggcgtggcg tcgacgacga
     1381 tcgtgtcgcc tgcctcgaag gccgcctgca gcatccgctc cgcaagcggg tcctcgatct
     1441 ggtgctggat ctcgcgacgc agcggacgcg ctccgaatga aaggtcgtac cccttcacga
     1501 tgatcgcgtc cttggcggca tcggtcacca ccagctccat ctgctgcccc gccagctggg
     1561 acttcacgcg ctccagcagc aggtccacga tctcgcggat ctcggcgcgg gtcagctcgc
     1621 ggaagacgac cgtcgcgtcg atgcggttga ggaactcggg ccggaagacg ttcttcagct
     1681 cgccgaggac cttctccttc atgcgctcgt agtccgccgt cgcctcggcg acggaaccgg
     1741 cgtcgccctc tgcgcggaag ccgatgcgcg aatccttctg cagcagctgg gcgccgacgt
     1801 tgctggtcat gatgatgatc gtgttgcgga agtcgacgcg gcggcccttg gcatcggtaa
     1861 ggtgcccgtc ctcgaggatc tggaggagga tgttgaagac ctccgggtgt gccttctcga
     1921 tctcgtcgag caggaccacg ctgtagctct tgcgacggac cgcctcggtc agctggccac
     1981 cttcgtcgta gccgacgtag ccgggaggcg cgccgaccag ccgcgaaacg ttatggcgct
     2041 ccatgaactc gctcatgtcg atcttcacca aggcgtcctc ggagccgaac atgaactcgg
     2101 ccagtgcccg ggcgagctct gtcttgccga cgccggtggg gcccatgaag atgaatgagc
     2161 cgatcggccg cttcggatcc ttcagcccgg cgcgtgcacg gcggaccgcc ttggagatga
     2221 tatcgatcgc ctcctgctgc ccgatcacgc gcttgtggat cgcgtcttcc atgtggagga
     2281 gccgctccga ctcctcggcg ctgatgcgga tcaccgggat gccggtccac atgctgacga
     2341 cctgggcgat ctcctcctcg gtgaccaccg gcacgtcctt ggcggcctcc tcgcgccagg
     2401 cggtacgcag cgcctcgata gcctccttgc cctgcgcctc ctcatcgcgg agtcgtgcag
     2461 ccgcctcgta gtcctgggcg gcgattgcct cgtccttctg gcgcgtcacg tcctcgaggt
     2521 ccttctgcgc ctccttgatc ttgggcgggg ccttcgactc gcgcaggcgc acgcgggacc
     2581 cggcctcgtc catcaggtcg atcgccttgt ccggcagcgc gcggtcggcc acgtagcgaa
     2641 ccgagaggtc cacggccgca cgcaccgcct cgtcgctgat cttgacgtgg tgatgctcct
     2701 cgtagcggga gcggatgccg aagaggatcg cgaccgcctc gtcgaccgtg ggctcctcga
     2761 ccatcaccgg ctggaagcga cgctccaggg cggcgtcctt ctcgatgtac ttgcggtact
     2821 cgtcgagggt ggtggcgccg atgcactgca gctcgccgcg cgacagcggc ggcttcagga
     2881 tgttggcggc gtcgatcgcg ccctccgcgg cgcctgcccc caccagcgtg tgcagctcat
     2941 cgatgaagag gacgctgtcg ctgctgctgc gcagctcctc gatgatcttc ttgaggcgct
     3001 cctcgaactc gccgcggtac ttggttccgg ccaccaatga gccgatgtcg agcgtcagca
     3061 cccgcttgtc ggcgagcgtc tcggggacgt cgccgctcac gatgcggtgc gccagcccct
     3121 ccgcgatcgc ggtcttgccg actcccggct cgccgatcag ggcagggttg ttcttggtgc
     3181 gccgactcag aatctggatg acgcgctcga tctccttttc gcgcccaatc accgggtcga
     3241 gcttgccgga gcgggcggcc tcggtgaggt tgatgcccag ctggtcgagg gtcggggtct
     3301 tcgatggccg cttcgtctcc tgcgccggcg agacgctggt gctctggctc aggacgtgga
     3361 tcacctggtg gcgcaccttg tcgaggttga cgcccaggct ctcgaggacg ccggcggcga
     3421 tcccctcgcc ctcgcggacg aggccgagca gcaggtgctc cgtgccgatg tagttatggc
     3481 cgaggcggcg tgcctcgtcg atggccagct cgatgacgcg cttggcgcgt ggcgtcaggc
     3541 ccacttcgcc gaccaccggc cgatctcccc ggccgatgat gaattcgacc gcggttcgta
     3601 ccttcgagag ctcgacgttc atgttctcga ggacgcgcgc ggcgactccc tcgccctccc
     3661 ggaccaggcc gagaagcagg tgctcggtgc cgatgtagtt gtggttgaag cgctgcgcct
     3721 cgtcctgggc aagggtcagc accttgcggg cccgatccgt gaacttgtcg aagcgatcca
     3781 tttgcgcatc catccgtgac tgcccggagg cccaggctgg accgccgaac gtggccagag
     3841 tgtagagcac cactcccgcg cgatgccttc aataggtact acggactacg gtgacggttt
     3901 gctcgtggaa atgcccggcc agcgcgtgtg agcgaggtgc gggtcaggct cgagcgggtg
     3961 cggactccaa accagagcga cgttcgatct acgaagcctc ggagtctgct tcggccgtga
     4021 cggcctccgg ctcttcgccg acagcaaccg cgaccggctc ttcgggcgcg gcctcggtgg
     4081 ccggctcctc gggagcggcc tcggcta
//
LOCUS       DATCQR010000231         2678 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_214440, whole genome shotgun sequence.
ACCESSION   DATCQR010000231 DATCQR010000000
VERSION     DATCQR010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2678
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214440"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="VIN32_07750"
     CDS             complement(<1..186)
                     /locus_tag="VIN32_07750"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEY8922505.1"
                     /translation="MAAPGFEAVLVDLDGEVATVTLNQPEKRNPLSTAMVRDLGAALA
                     WCRGEPAVRVVVLTGAGD"
     gene            complement(198..728)
                     /locus_tag="VIN32_07755"
     CDS             complement(198..728)
                     /locus_tag="VIN32_07755"
                     /inference="COORDINATES: protein motif:HMM:NF014689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump"
                     /protein_id="HEY8922506.1"
                     /translation="MSLSVGLSNFAGSIGIGLSGVDARTRLRVGIAFGLFEGLMPVLG
                     LFIGQAVAGTIGSYGRYLGAGLLVLTGFYTIWQSFRTGKVERDEEKGILDTRQLLIIG
                     AALSLDNLIVGFALGVYNVSIVLAAVVMAIVSVGLSLLGLELGSRLGSRVGEYSEMAG
                     GGVLVLVGIALGVGLI"
     gene            complement(755..1693)
                     /locus_tag="VIN32_07760"
     CDS             complement(755..1693)
                     /locus_tag="VIN32_07760"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY8922507.1"
                     /translation="MALRLGVSFDAGSSLASAIERARLADHLGYDSLWASQLPPARDT
                     PLVLATYAAATSRIGLATAVLPIYTRHPTAMAQMALTLDEISGGRFRLGIGVSHQVTV
                     ESMWGLRLIHPVEAMREYAGIVRSLVTTGSVNVDGEQFTAHTAYTAPRRADLPIIISA
                     LSPRMLELAGEIADGVSLWMCAPDYIRDVVVPTVRKGREKAGKSLDGFEVEAAVPVCL
                     TTDRGAGLAAFRKTVERYASLPFYRKVLDRTFPDMSDSPGDPILQALAGIGDSAEVRA
                     TIERYREAGTTLPVLGPFAGHEGAAGFEATLEALAA"
     gene            1840..>2678
                     /locus_tag="VIN32_07765"
     CDS             1840..>2678
                     /locus_tag="VIN32_07765"
                     /inference="COORDINATES: protein motif:HMM:NF013106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEY8922508.1"
                     /translation="MGLARRGALSWEGGQKVAEAAVTLAAAALRGTGAFFHSMRLQLV
                     QGSAPAMRPQHLRWPDGRRFRASAVGRRRGYSTGGRPATQTAGTAAVVSTLLVIAVAV
                     GSFVSASALWVKYASDLPDAHSIALASLPQDSILYDVSGTVTLADIHRQGYRHYEQHL
                     TDMGTLLPQATIAVEDAKFYSEPGVDVGSIIRAAWVDYHDHAAVQGASTITQQLVKLR
                     LLGNQPSVERKAKEAVLALQVEQDFSKKQILEMYLNTAFYGNNAYGIQAAAQSYFHVP
                     TAKL"
BASE COUNT          456 a          917 c          916 g          389 t
ORIGIN      
        1 gtcgcccgcg ccggtcagga cgacgacccg gacggccggc tctccccggc accaggcgag
       61 ggccgccccg aggtcgcgga ccatcgccgt cgagagcggg ttgcgcttct cgggctggtt
      121 gagggtgacc gtcgccacct cgccgtcgag gtcgaccagc accgcttcga agccgggagc
      181 agccacttcg gaacctatta gatcagcccc acgccgagcg cgatgccgac cagcaccagc
      241 actccgcccc cggccatctc gctgtactcg ccgacccgcg agccgagccg gctcccgagc
      301 tcgagcccga gcagggagag gccgacgctg acgatcgcca tgacgaccgc cgccagcacg
      361 atcgagacgt tgtagacgcc gagcgcgaac ccgacgatca ggttgtcaag ggacagggct
      421 gcgccgatga tcaagagctg gcgggtgtcg aggatgccct tctcctcatc gcgctccacc
      481 ttcccggtcc ggaagctctg ccagatcgtg tagaaaccgg tcagcacgag gaggccggcg
      541 cccaggtagc ggccgtagga gccgatcgtg ccggcgaccg cctggccgat gaaaaggccg
      601 agcaccggca tcaggccttc gaaaaggccg aaggcgatgc cgactcggag ccgggtgcgg
      661 gcgtcgacac cggagaggcc gatgccgatc gagccggcga agttgctgag gccgaccgaa
      721 aggctcacca gcagcagtcc gagcaggttt ccgctcaggc ggccagcgcc tccagcgtgg
      781 cctcgaagcc ggccgccccc tcgtggccgg cgaagggccc gaggaccggc agggtggtgc
      841 ccgcttcccg ataacgctcg atggtcgccc tgacctcggc actgtcgccg atgccggcaa
      901 gcgcctggag gatcggatcg cccggcgagt cggacatgtc ggggaaggtc ctgtcgagca
      961 ccttccggta gaagggcagc gaagcgtagc gctcgacggt cttgcgaaag gcggcaagcc
     1021 cggcgccacg atcggtcgtc aggcagacgg gcaccgccgc ctcgacctcg aagccatcca
     1081 gcgacttgcc cgccttctcc cttcccttgc gcacggtcgg gacgacgacg tcccggatgt
     1141 agtcgggcgc gcacatccag agggagacgc cgtcggcgat ctcgccggcc agctcgagca
     1201 tccgcgggct gagcgccgag atgatgatcg ggaggtcggc gcggcggggc gcggtgtaag
     1261 ccgtgtgggc cgtgaattgc tccccgtcca cgttcaccga gcctgtcgtg acgagtgagc
     1321 ggacgatgcc tgcgtactcc cgcatcgcct ccaccggatg gatgagccgc aggccccaca
     1381 tcgactccac ggtgacctgg tggctaaccc cgatgccgag ccggaagcgg ccgccagaga
     1441 tctcgtcgag ggtcagcgcc atctgcgcca tcgccgtcgg atggcgggtg tagatcggca
     1501 gcacggcggt cgcgaggcct atcctcgagg tcgccgcggc gtacgtcgcc agcaccagcg
     1561 gggtgtctcg agcggggggc agctggctgg cccagagaga gtcgtaacca aggtgatcgg
     1621 ccagcctggc gcgttctatt gctgaggcga gcgagctccc ggcgtcaaag ctgacaccga
     1681 gccggagggc cattcgcgaa ggatagccgt tgaccgtaac ccgaccggcc agcagagcgt
     1741 tttagcttca ttcgttgaca ctagacctga tagcgttagc atcaccttta gtttttaaag
     1801 cgattacagt attgtcgtgg ggggctccag ggaaccgaca tgggactagc tagacgcggg
     1861 gcgctcagct gggagggcgg ccagaaggtc gccgaggcag cggtgacgtt ggctgcggcc
     1921 gcgcttcggg gcaccggcgc gttctttcac tcgatgcgcc tgcagctcgt ccaggggagc
     1981 gcgccggcga tgcggccgca gcacctgcgc tggccggacg ggcgccgttt ccgagcttcc
     2041 gcggtcgggc ggcgccgggg ctactcgacc ggcggccggc ccgcgacgca gacggccgga
     2101 acggccgccg tggtgagcac gctgctggtc attgcggtcg cggtgggctc cttcgtcagt
     2161 gcttccgcgc tgtgggtcaa atacgcttcc gacctgcccg acgcccactc gatcgcgctc
     2221 gcctcgctgc cccaggactc gatcctctac gacgtctcgg gcacggtgac gctggccgac
     2281 atccaccgcc agggctaccg ccactacgag cagcacctga ccgatatggg cacgctcctg
     2341 ccccaggcga cgatcgcggt cgaggacgcc aagttctaca gcgaacccgg ggtcgacgtc
     2401 ggctccatca tccgcgccgc gtgggtcgac taccacgacc acgccgcggt gcaaggcgct
     2461 tcgacgatca cccagcagct ggtcaagctg cggctcctgg gcaaccagcc gagcgtcgaa
     2521 cggaaggcga aggaggccgt gctggccctc caggtggagc aggacttcag caagaagcag
     2581 atcctcgaga tgtacctgaa caccgccttc tacgggaaca atgcctacgg cattcaggcc
     2641 gccgcccaga gctacttcca cgtcccgacc gcgaagct
//
LOCUS       DATCQR010000232         2335 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_198197, whole genome shotgun sequence.
ACCESSION   DATCQR010000232 DATCQR010000000
VERSION     DATCQR010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2335
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198197"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..956)
                     /locus_tag="VIN32_07770"
     CDS             complement(<1..956)
                     /locus_tag="VIN32_07770"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY8922509.1"
                     /translation="MSDAQQFDILILGGGMSYVGAIRAAQLGLKVGLVERDRMGGTCL
                     NRGCIPTKAMLETADLLHRVTEQGAEFGLAGGEGVTLDYPALGKRRDAVVEKHVKGVE
                     FLMKKNNVTILRGSGTLTSPTSVHVSGGESGEVDASGTDLILATGSAPRSLPGLDVDG
                     ERIITSDEALLRETVPARVAIVGAGAIGVEWASLYSDLGAEVHLIEYLERIVPLEDAD
                     VSKELTRIFKKRNVKIYAASSIDSKSIERTKRGVHFAVEARDGKTRVEMETDVILVAV
                     GRRPVTDGIGLEDIPGVEMERGFVKVNGFMQTGVPHLYAIGD"
     gene            complement(953..1393)
                     /locus_tag="VIN32_07775"
     CDS             complement(953..1393)
                     /locus_tag="VIN32_07775"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEY8922510.1"
                     /translation="MIDEFRHRTRLEVRLGDLDPFGHVNNAVIATYVEQGRVLYLRDV
                     LRTGVDPVSMPFILARIEIDYLSQVMFSDAVEVGSRVDWIGRTSIGMSHLLINQEARE
                     LARSEAVLVAFDYDVEKPIPVADEWRAIMVAYEGRPLDRPVTPA"
     gene            complement(1418..>2335)
                     /locus_tag="VIN32_07780"
     CDS             complement(1418..>2335)
                     /locus_tag="VIN32_07780"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEY8922511.1"
                     /translation="RLLLIGHMDTVFEPGTAAERPFKVDGDRATGPGVSDMKGGLLAG
                     LHGLAALRSLDRRPSVTFVANPDEEIGSPFSTAHIRGLVAEHDAALVLECARANGDIV
                     SARKGIADYEVTITGRAAHAGVEPEKGRSAILEAAHQILALHALNGRWPSATVNAGVI
                     EGGTRPNVVAASCRMEVDLRAASASEFEMAAAEMEGIVTHPTLEGVSATLRKAAFHPP
                     MERTAGNARLAELAISIAGELGFELRDALTGGASDANTTTAAGLPTLDGLGPVGGDDH
                     SADEWLDLASVVPRTTLLAALIDRVGEAI"
BASE COUNT          339 a          810 c          796 g          390 t
ORIGIN      
        1 atgtcgccga tcgcgtacag gtgcgggaca ccggtctgca tgaagccgtt gaccttgacg
       61 aagccgcgct ccatctcgac gccgggaatg tcctccaggc cgatcccatc cgtcacgggg
      121 cgcctgccga ccgccaccag gatgacgtcc gtctccatct cgacgcgcgt cttgccgtcg
      181 cgcgcctcga ccgcgaaatg gaccccgcgc ttggtgcgct cgatgctctt ggagtcgatg
      241 ctcgacgctg cgtagatctt gacgttccgc ttcttgaaga tgcgggtcag ctccttgctg
      301 acgtccgcgt cctcgagcgg cacgatgcgc tcgaggtatt cgatcaggtg cacctccgca
      361 ccgaggtcgc tgtacaggct ggcccattcg acgccgatgg cgccggcgcc cacgatggcc
      421 acgcgcgccg ggaccgtctc gcgcagcagc gcctcgtccg aggtgatgat ccgctcgccg
      481 tcgacgtcga gccccggcag gctgcgcggc gcagagccgg tggccaggat caggtcggtg
      541 ccggaggcgt caacctcacc agactcgccg ccgctgacgt gcacgctggt cgggctggtc
      601 agcgtcccgc tgccgcgcag gatggtgacg ttgttcttct tcatcaggaa ctcgacgccc
      661 ttgacgtgct tctcgacgac ggcgtcgcgc cgcttgccga gggcggggta gtcgagggtg
      721 acgccctcgc cgccggccag gccgaactcc gcgccctgct cggtcacccg atgcagcagg
      781 tcggcggtct cgagcatcgc cttggtgggg atgcagccgc ggttcaggca ggtgccgccc
      841 atcctgtcgc gctccaccag gccgaccttg agtcccagct gcgcggcccg gatggcgccc
      901 acgtagctca tccctccgcc caggatcagg atgtcgaact gttgcgcgtc gctcatgcag
      961 gggtcaccgg tcgatcgagg ggccgtccct cataggccac catgatggcg cgccactcgt
     1021 cggctactgg gatcggcttc tcgacgtcgt agtcaaaggc gaccagcact gcttcggatc
     1081 gggccaattc gcgtgcctcc tggttgatga gcaggtgcga catgccgatg ctggtgcgtc
     1141 cgatccagtc gacccgggag ccgacctcca ccgcatcgct gaacatcacc tgcgacaggt
     1201 agtcgatctc gatcctggcc aggatgaacg gcatgctgac cggatcgacg ccggtcctca
     1261 gcacgtcccg caggtagagg acgcgtccct gctcgacgta cgtggcgatg accgcattgt
     1321 tgacgtggcc gaaagggtca aggtcgccaa ggcgcacctc gaggcgcgtg cggtgacgga
     1381 actcatcgat cacgggaacc cgattctaca cgggcggtca gatcgcttcg ccgacccgat
     1441 cgatcagcgc ggcgagcagg gtggtgcgcg gcacgacgct cgccaggtcg agccattcgt
     1501 cggccgaatg gtcgtcgcca ccaacggggc cgagcccgtc cagggtgggc agtcccgccg
     1561 ccgtggtggt gttcgcgtcg ctcgccccgc cggtcagcgc atcgcgcagc tcgaagccaa
     1621 gctccccggc gatggagatg gcgagctcag ccaggcgggc attgcccgcg gttcgctcca
     1681 tcggcggatg gaaggctgcc ttgcggaggg tcgcagagac gccctcaaga gtgggatgcg
     1741 tgacgatgcc ctccatctcg gccgcagcca tctcgaactc cgaggcgctc gccgcccgca
     1801 ggtcgacctc catccggcag cttgccgcga ccacgttcgg acgggtcccg ccctcgatca
     1861 cgccggcgtt gaccgtcgcg ctgggccagc gcccgttcag cgcgtggagg gcgaggatct
     1921 ggtgagctgc ctcgaggatg gcgctgcggc ccttctctgg ctcgacgccg gcgtgtgccg
     1981 cacggcccgt gatggtgacc tcgtagtcgg cgatcccctt gcgggcgctg acgatgtcgc
     2041 cgttggcgcg ggcgcactcc agcaccaggg ccgcgtcgtg ttcggcgacg agcccgcgaa
     2101 tatgcgcagt gctgaagggg gagccgatct cctcgtcggg attggcgacg aaggtgaccg
     2161 atggtcgacg gtcgaggctg cgcagcgcgg caagcccgtg caggcccgcc agcaggccgc
     2221 ccttcatgtc gctgacgcca ggccccgtgg cgcggtcgcc gtccaccttg aacggccgct
     2281 cggcggcggt ccccggctcg aagaccgtgt ccatgtggcc gatcaggagc aggcg
//
LOCUS       DATCQR010000233         4790 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_44446, whole genome shotgun sequence.
ACCESSION   DATCQR010000233 DATCQR010000000
VERSION     DATCQR010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4790
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44446"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1819)
                     /locus_tag="VIN32_07785"
     CDS             complement(<1..1819)
                     /locus_tag="VIN32_07785"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEY8922512.1"
                     /translation="MPPATHRDLSTEDLDRPIRSVLPDLARGAATFGRLGIATPRQAL
                     FYLPFRYDDFSDLRPLGELEADEKQSARVRVTDLKIEPGFGRRPQRVIAQLADESGTA
                     EAIWFGRRYVERRLEKGTEVVVSGKVTFRGWRPQFTSPDFSPVGHESIHTARVVPIYR
                     LVGGVTQRRVRELLARVLERALPAIEDPLQPAERGELPVLADALRAAHFPEEAADVPA
                     ALDRLAFDELLALQLSMAQAREARAGERAASVDVDDRELAALLEALPFALTGDQRRAV
                     DEIVADLASDEPMRRLLQGDVGSGKTAVAAVALAAVVRAGWQGALMAPTEILARQHHL
                     GLAPLLEALGVRAEFLSGSLGTAVKGRIHDAIAAGEAEVVIGTHAVIAEAVSFDRLAL
                     AVVDEQHRFGVAQRAALQAKGAGREPHLLALTATPIPRTLALTFYADMAISTIREMPP
                     GRRSIRTEVRNRHAMPRIEAFLASEAAAGRQSFVVVPLVAESEALSAASAETEFERLQ
                     AALPAMRIGLVHGQQRADVRDATMAAFSAGELDLLVATTVVEVGIDVPNASVMLIEDA
                     DRFGLAQLHQLRGRVGRGEQQSFCILLTDAVDDRSRERLE"
     gene            complement(1825..2400)
                     /locus_tag="VIN32_07790"
     CDS             complement(1825..2400)
                     /locus_tag="VIN32_07790"
                     /inference="COORDINATES: protein motif:HMM:NF025052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY8922513.1"
                     /translation="MQSSTAALVIVSGAPGSGKSTLARRLSADLHLPLISRDQLKEAL
                     ADAMGSPSDVPASMLLGAGSVAVLHLVARQLLEASVGLIIESNFRRGLSETELLPLLA
                     WSDPALIHCTASPKLIQRRYAERHARGERHAAHLDADRAAGLAVDLADGRFEPLNLAI
                     PMLVVDTTDGWQPAYEEIRDFATLPRAALAR"
     gene            2487..2678
                     /gene="rpmB"
                     /locus_tag="VIN32_07795"
     CDS             2487..2678
                     /gene="rpmB"
                     /locus_tag="VIN32_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020675729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEY8922514.1"
                     /translation="MARACDICGKRPITGWNPQSVGMNRKRALRRWLPNLQPMVVQRN
                     GSPVRIKACSRCIRTAEKV"
     gene            complement(2680..3129)
                     /gene="dtd"
                     /locus_tag="VIN32_07800"
     CDS             complement(2680..3129)
                     /gene="dtd"
                     /locus_tag="VIN32_07800"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002605210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HEY8922515.1"
                     /translation="MRLVVQRVSRAEVRADRVVLGAIGRGAAVLVGVGVGDTPELADR
                     LADRLIGLRYFEDADGRTNLAIDDVGGSFLVVSQFTLLTDLRRGRRPSFAPAAAAAVA
                     EPIVDRFVERLREAGRDVATGRFGAEMELELVNDGPFTLVVEAGAEA"
     gene            complement(3134..4711)
                     /locus_tag="VIN32_07805"
     CDS             complement(3134..4711)
                     /locus_tag="VIN32_07805"
                     /inference="COORDINATES: protein motif:HMM:NF024598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HEY8922516.1"
                     /translation="MTLFLRGRPLVIALLTAMIVIGCSAPGPEPSPTPRPPRDVTVTV
                     VDRTNQAPVPNATVHSGSATAITGADGTAILRTVGGTSVAASADGFDAGTGTVPDEKG
                     LTIGLRPNVVSGAVTDGDGKPIAGVRVFVEGAAQPVVTDAKGRYELAEVPEQGTLIYK
                     MPGYRLGVIPIDAQMTKDVALTPFAARALYSPSAIFEAAGRLDAQLDLIDRTEANAMV
                     IDVKETAGTLYWATDLPAATAVGAVVDHPLFQLDELLPKLKERGIYTIARLVVMKDNT
                     LGKARPELAVNNVATGKPWRDYRGGIWLDPYNAGVAEYIAALAGDLADKGFDEVQLDY
                     VRFFSDGDYATADTNLPNTQSFRLPAIRRLFRIVSDALLTKRAFLSADVFPISFIATD
                     DQGIGQRPEVIMPYVDYFSPMVYPSHYGPFTFHFENPNDHPYQVIDETLKIMNQERAG
                     LRMVIRPWIQDFGYGPFPPYTAAQVLQEMKAANDNGAQGWMIWNAQARFTEAALAPPR
                     EGESSGPITSPLPSPGG"
BASE COUNT          722 a         1678 c         1669 g          721 t
ORIGIN      
        1 cctcgagccg ctcgcgcgat cgatcgtcga cggcgtcggt gagcaggatg cagaacgatt
       61 gctgctctcc gcgtcccacc cgtccgcgca actggtggag ctgggcgagg ccaaagcggt
      121 cggcgtcctc gatcagcatc accgacgcgt tcggcacgtc gatcccgacc tcgaccacgg
      181 tggtcgccac gagcaggtcc agctccccgg ccgagaaggc ggccatggtc gcgtcgcgca
      241 cgtctgcgcg ctgctgcccg tggacgaggc cgatccgcat cgccggcaac gccgcctgga
      301 gacgttcgaa ctccgtctcg gcggaggcag cgctgagcgc ctcgctctcg gcgacgagcg
      361 ggacgacgac gaacgactgc cggccggcgg cggcctctga ggcgaggaac gcctcgatgc
      421 gcggcatcgc gtgccgattg cgcacctcgg tccggatcga gcggcgtccg ggtggcatct
      481 cgcggatcgt gctgatcgcc atgtcggcgt agaaggtcag cgccagcgtg cgcgggatcg
      541 gcgtggcggt cagcgccagc aggtgcggct ctcggccggc acccttggcc tggagcgccg
      601 cccgctgtgc caccccgaat cggtgctgct cgtcgaccac cgccaacgcc agccggtcga
      661 atgagaccgc ctccgcaatg acggcgtgcg tcccgatcac gacctccgcc tcccccgccg
      721 cgatcgcgtc gtggatgcga cccttgacgg cggtcccgag cgatcccgac aggaactcgg
      781 cccgcacgcc gagcgcctcg agcagaggcg ccaaccccag gtggtgctgc cgcgcgagga
      841 tctccgtcgg cgccatcagc gcgccctgcc agcccgctcg caccacggcc gcgagggcga
      901 ccgcggccac cgccgtcttc ccgctcccca cgtcgccctg caggaggcgc cgcatgggct
      961 cgtcggatgc gaggtcggcg acgatctcgt cgacggctcg ccgctggtcg ccggtaagcg
     1021 cgaatggcag cgcctccagc agcgcagcca gctcgcggtc gtcgacatcg acgctggctg
     1081 ctcgctcgcc ggcccgtgcc tcgcgggcct gcgccatgct gagctggagg gcgaggagct
     1141 cgtcgaaggc cagccgatcg agtgcggccg gcacgtccgc cgcctcctcg ggaaagtggg
     1201 cggcgcgcag ggcatcggca agcaccggaa gctcgccacg ctccgccggc tgcagcggat
     1261 cctcgatggc gggcagcgcc cgctcgagca ccctggccag gagctctcgg acccggcgct
     1321 gggtgacgcc tccgacgagc cggtagatgg ggacgacgcg cgcggtgtgg atcgactcgt
     1381 gccctaccgg tgagaagtcg ggcgacgtga actgcggccg ccagccgcgg aaggtgacct
     1441 tgccgctgac caccacctcg gttcccttct ccagccggcg ctcgacatag cgcctcccga
     1501 accagatcgc ttcggccgtc ccgctctcat cggccagttg ggcgatgact cgttgcggtc
     1561 gcctgccgaa gccgggttcg atcttgaggt cggtcaccct cacccgcgcc gactgcttct
     1621 cgtcggcttc cagctccccc agtggccgca ggtcgctgaa gtcgtcgtat cggaagggga
     1681 ggtagaagag tgcctggcgc ggggtcgcga tgcccagccg tccaaaggtg gctgcaccgc
     1741 gggccaggtc gggcaggacc gagcggatcg gccgatccag gtcctccgtc gacaggtctc
     1801 ggtgggtcgc gggaggcacc agcgtcatcg ggccagagcg gcgcgcggga gagtggcgaa
     1861 gtcgcgaatc tcctcgtagg ccggctgcca gccatcggtc gtgtcgacga ccagcatcgg
     1921 gatcgcgaga ttgagcggtt cgaagcgccc atccgcgaga tccacggcga gcccggcggc
     1981 gcggtcggcg tcgaggtgcg ccgcgtggcg ctcccctcgg gcgtgccgct ccgcgtaccg
     2041 ccgttggatg agcttgggcg atgcggtgca gtggatcagg gctgggtcac tccaggcgag
     2101 caggggaagg agctctgtct cggacaaccc gcggcgaaag ttgctctcga tgatgagtcc
     2161 aacgctggct tccagcagct ggcgagcaac gaggtggagc actgcgaccg aaccggcacc
     2221 gagcagcatc gatgcaggga cgtccgatgg agatcccatg gcgtcggcga gcgcctcctt
     2281 gagctgatcc ctcgagatga ggggcaggtg caggtcagct gacagcctcc gcgccagcgt
     2341 cgacttgccg ctcccgggag caccggaaac gatcaccagc gccgccgtgc tcgattgcat
     2401 ggcccgatcg tagcgtgcta ggatcgggcc gttccggcgg cgccgaggca ggtgcgccgg
     2461 ttcgttcatg aaacggagac tgacccatgg ctcgagcctg cgatatctgc ggcaagcgac
     2521 cgatcaccgg ctggaatccc cagtcggtcg gcatgaaccg caagcgcgcg ctgcgccgtt
     2581 ggctgcccaa cctgcagccg atggtggtcc agcgcaacgg ctcgcctgtc cgcatcaagg
     2641 cctgctcccg ctgcattcgg accgccgaga aggtctgact caggcttctg cgcctgcctc
     2701 gacaaccagc gtgaacggtc catcgttcac gagctcgagc tccatctccg ccccgaaccg
     2761 cccggtcgcg acgtcccgac cagcctcgcg cagccgctcc acgaagcgat ccacgatcgg
     2821 ctcggccacc gcagcagcgg cggctggcgc gaagctgggt cgacggccgc gccgcaggtc
     2881 cgtcaggagc gtgaactggc tcaccaccag gaacgagccg ccgacatcgt cgatggcgag
     2941 gttcgtccgc ccatccgcgt cctcgaagta gcggagtccg atgagccgat ccgccaggcg
     3001 gtcggccagc tctggcgtat cccccactcc aacccccacc agcacggcgg caccacgacc
     3061 gatcgccccc aggacgactc tgtcggcacg aacctcggcc cggctgaccc gctgcacgac
     3121 caggcgcacg gacctagccg cccggcgaag gcagcgggct ggtgatcggc cccgaggact
     3181 cgccctcgcg gggtggcgcg agcgccgcct ccgtgaatcg ggcctgcgca ttccagatca
     3241 tccagccctg ggcgccgttg tcgttggcag ccttcatctc ctggagcacc tgggcagcgg
     3301 tgtacggcgg gaacgggccg tagccgaagt cctgtatcca ggggcggatc accatccgca
     3361 ggccggcccg ctcctggttc atgatcttga gggtctcgtc gatcacctgg tatggatggt
     3421 cgttcgggtt ctcgaagtgg aaggtgaatg ggccgtagtg gctcgggtag accatcggcg
     3481 agaagtagtc gacgtacggc atgatgacct ccggtcgctg gccgatcccc tggtcgtcgg
     3541 tggcgatgaa gctgatcggg aagacgtcgg ccgagaggaa ggcgcgcttg gtgaggagcg
     3601 cgtcggagac gatccggaag aggcggcgaa tggccgggag acggaaggac tgcgtgttcg
     3661 gcaggttcgt atcggccgtg gcgtagtcgc cgtcgctgaa gaagcgcacg tagtcgagct
     3721 gcacctcgtc aaagcccttg tcggcgaggt cgccggccag cgccgcgatg tactcggcca
     3781 cgcccgcgtt gtagggatcc agccagatgc cgccgcggta gtcacgccat ggcttgcccg
     3841 tcgcgacgtt gttgacggcc agctccggac gggccttgcc cagggtgttg tccttcatca
     3901 ccaccagccg ggcgatggta tagatgccgc gctccttcag cttcggcagc agctcgtcga
     3961 gctggaacag tggatggtcc acaaccgcgc cgacggccgt cgcggccggc aggtcggtgg
     4021 cccagtacag ggtgccggcg gtctccttca cgtcgatcac catggcgttg gcctcggtcc
     4081 ggtcgatcag gtcgagctgt gcgtccagtc ggccggcagc ctcgaagatg gccgagggcg
     4141 agtagagcgc ccgcgccgcg aacggtgtca acgcaacgtc cttggtcatc tgcgcatcga
     4201 tcgggatcac tcccagccga tacccgggca tcttgtagat gagggtcccc tgctccggga
     4261 cctcggccag ctcatagcgg cccttggcgt cggtcacgac cggctgcgcg gccccttcca
     4321 cgaagacgcg caccccggcg atcggcttcc cgtcgccgtc ggtgaccgcc ccgctcacga
     4381 cattcggacg gagcccgatg gtgagcccct tctcgtcggg gacggtgccg gtccctgcgt
     4441 cgaacccatc ggcgctcgcc gcgacgctcg tgccgccgac cgtgcggagg attgcggtcc
     4501 catcggcgcc ggtgatggcc gtggcgcttc cgctatgcac ggtggcgttc ggcacaggtg
     4561 cctggttggt ccgatccacc accgtgacgg tgacgtcacg cggcggccgc ggggtcgggc
     4621 ttggctcggg gcccggcgcg gagcaaccga tcacgatcat ggcagtcagc agcgcgatga
     4681 ccagaggccg gccgcgcagg aacagggtca cgccaccacc gcggctccgg cggcgcgaag
     4741 cgcctggacg gtcggcgcca ggtcgccctc ctcgccaaac agcgccgatc
//
LOCUS       DATCQR010000234         1872 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_136886, whole genome shotgun sequence.
ACCESSION   DATCQR010000234 DATCQR010000000
VERSION     DATCQR010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1872
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136886"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..779)
                     /locus_tag="VIN32_07810"
     CDS             complement(<1..779)
                     /locus_tag="VIN32_07810"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEY8922517.1"
                     /translation="MANLRTLLIVAGLTIAFACLAAMAADDLSAASRRVRRRVGRLVR
                     PESVRVALAQAGLTSIPVPAWILSRTGLAVAAAAIAWAWFGLPVLGLIAFLVVYHLIG
                     TALEFRRRGFEARHQEALLEAVRHGIAVMSRAGNATSMLEALADGGPFEVRPLFKEIV
                     LVGGSGPAGETFVRELERIRDRVADPLFDDVVLALTLHWRQGGKLVPALEAVVNDWSE
                     TLRLQREAKALRSGVEASVVLLALLPFVFLVTLQLLAPTLLA"
     gene            complement(766..>1872)
                     /locus_tag="VIN32_07815"
     CDS             complement(766..>1872)
                     /locus_tag="VIN32_07815"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEY8922518.1"
                     /translation="YIQHGALGPLGELLAKEGVEDIHMNGLDHGYLEYGDHREPLPAS
                     FESEEDLISIVRFYAEQSGRRFDVSSPIVTVTMRDGSRLNAVLPPIAKPLVITIRKQQ
                     LRRFLSLSDLVRSGTIPARAVPLLEAAVRARLNIILSGPTGTGKTTLARVLALMIPEG
                     ERTCVLETETELWLHELRPQFFSLEERDANVEGVGRITMQDLFQRAALRQRPKRIIVG
                     EVRGTEALDMIHAMSSGHDGSLTTLHASNPQTALDRLEVLAMSADRNLAPHVVRRMIG
                     AGVDLIVHLSTYHRGDQELRRLGSLAFVAENRENDLGSPVVSGLASYRILDDSWDWRR
                     EALVHMPDKVWKKLLAAGVNPEDVVREVLGNGES"
BASE COUNT          352 a          597 c          609 g          314 t
ORIGIN      
        1 gcgagcagtg tgggcgccag cagttgcagc gtgaccaaga acacgaatgg gagcagggcg
       61 agcagcacca ccgacgcttc gacgcccgac cgcagcgcct tggcctcgcg ctgaaggcgc
      121 agtgtctctg accagtcgtt aacaaccgcc tcgagcgccg ggacgagctt gccgccctgt
      181 cgccagtgca gagtcagcgc aagtacaaca tcgtcgaaga gcgggtcggc tacgcggtcg
      241 cggatcctct ccagctcgcg gacgaacgtc tcgcctgcgg gaccgctgcc accaacgagg
      301 acgatttctt tgaagagcgg ccgaacctcg aacggaccgc cgtcggcgag cgcttcaagc
      361 atcgaggtcg cgttgcccgc acgcgacatg accgcaatcc cgtggcgcac ggcctcgagg
      421 agcgcctcct ggtgtcgagc ctcgaagccg cgacgtctga actcaagcgc ggttccgatc
      481 aggtggtaca cgacaagaaa tgcgatcagg ccgaggactg ggagcccgaa ccacgcccag
      541 gcgatggccg ctgcggcgac cgcgagcccg gtccgagaca gaatccacgc aggcacgggt
      601 atcgatgtga gacctgcctg ggcaagcgcg acgcggaccg attccgggcg taccagccgt
      661 ccaacgcgac gacgcacccg tcgggaagca gcactcagat catccgccgc catggcggcc
      721 agacaggcga aggcgatcgt caggccggca acgataagaa gggtcctaag attcgccatt
      781 cccgagcacc tctcggacca cgtcctcggg attgactcca gctgcgagca gctttttcca
      841 caccttgtcc ggcatgtgca ccagggcctc tcgacgccag tcccaggagt catccaggat
      901 gcgatagctg gcgaggcctg agactacggg cgagccgaga tcgttctctc ggttctcggc
      961 tacgaacgcg agtgagccga ggcggcgaag ttcctggtcg ccgcgatggt aagtactcag
     1021 atgcacgatg aggtcgacgc cagctccgat catccgtcgc acgacatgag gcgcaaggtt
     1081 tcggtcggcc gacattgcaa gcacctcaag gcggtccagt gctgtctgcg ggttgctcgc
     1141 atgcagcgtg gtaaggctgc catcgtgtcc gctggacatc gcatggatca tgtcgagcgc
     1201 ctctgtccct cggacttcgc cgacgatgat ccgcttggga cgctgacgca aagcggcgcg
     1261 ctgaaataga tcctgcatgg tgattcggcc gacgccctca acgtttgcgt cgcgctcttc
     1321 gaggctgaaa aactgcggac gcagttcgtg cagccagagt tcggtctctg tctcgaggac
     1381 gcaagtgcgc tcgccctcgg ggatcatcag cgccagcact cgcgccagcg tcgtcttgcc
     1441 ggtgccagtg ggtccgctga gaatgatgtt cagccgagcc ctcactgcag cttcgaggag
     1501 cgggacggcg cgggccggaa tcgttcccga cctaaccagg tcgctgaggc tcaggaaccg
     1561 gcgcagctgc tgcttgcgaa tggtgattac gaggggcttg gcgatcggcg ggagcactgc
     1621 gttgagccgg ctgccgtcgc gcatcgtgac cgtaacaatc gggctcgaaa cgtcgaacct
     1681 gcgtcctgac tgctcggcgt agaaccggac gatgctgatg aggtcctcct ccgactcgaa
     1741 ggacgccggc aatggctcgc ggtggtcgcc gtactcgagg tagccatggt cgaggccgtt
     1801 catgtggatg tcctcaaccc cttccttggc caggagctcg ccgagcggtc caagcgcgcc
     1861 gtgttggatg ta
//
LOCUS       DATCQR010000235         4011 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_122273, whole genome shotgun sequence.
ACCESSION   DATCQR010000235 DATCQR010000000
VERSION     DATCQR010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4011
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122273"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..949)
                     /gene="galE"
                     /locus_tag="VIN32_07820"
     CDS             complement(<1..949)
                     /gene="galE"
                     /locus_tag="VIN32_07820"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009675919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEY8922519.1"
                     /translation="MRILVVGGAGFVGSTSVETFVEAGHDVVVYDDLSSGHASAVVRP
                     ARMVRGQIEDQGHLEHVLREERIDAVLHCAAKSLVGESMADPGLYYRKNVGGGVALLE
                     AMRVTGVNRLVFSSTAAVYGEPRRVPIAESDRTEPINPYGASKLAFEGAMRWFAASHD
                     FQAISLRYFNVAGATERNGEDHEPETHLMPLMLRVAAGEATHVQIFGQDYPTPDGTCI
                     RDFIHVRDLAAAHLRALEVTGEGDPSFEVYNLGSAAGFSVREVVEAARKVTGRAIPAR
                     VVKRRTGDPPVLVASSRRARLALGWHPEHSKLEQMLADAW"
     gene            complement(975..1703)
                     /gene="deoC"
                     /locus_tag="VIN32_07825"
     CDS             complement(975..1703)
                     /gene="deoC"
                     /locus_tag="VIN32_07825"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEY8922520.1"
                     /translation="MTALARTARLEHRLTRPTASLADLHVAAGLALEHQLATLLVSPW
                     LVKPAARLLSRTPVAVGTVIGFPHGGQVAAVKAFEASTALEHGATQLDVMLNAGALVS
                     GDDEAVLNDMLAVVEMAQSAMATCGVIVRAGPVSEELLRRACRLSARSAAAYVVTSDG
                     REQADATVEQARFLHGVVGGHLQVKAAGEFTEMSQLVEAVAAGADRISSTFSPQLAAE
                     FMAWQQQPAPKAAGSPADVGAAAR"
     gene            complement(1779..3137)
                     /locus_tag="VIN32_07830"
     CDS             complement(1779..3137)
                     /locus_tag="VIN32_07830"
                     /inference="COORDINATES: protein motif:HMM:NF025112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEY8922521.1"
                     /translation="MRDGDPLRIDLPPAVASVLDALRQGGAESALVGGSVRDLVRGDQ
                     PGDWDVATSAPPESVAARFPGATWENPFGTVTVRDPAGGRGIEVTTYRVEGGYRDRRR
                     PDEVRWGQSLADDLARRDFTINAMAWLPDAAADGSGRLVDPHGGAADLEAGLLRAVGD
                     PDERFDEDALRLLRAVRLANRFQLRVDPPTEAAILANAPNAAGLSGERVRDELMRILA
                     GAAPPSRAFELMERLGLLDVLLAELAALRGVPQTKRLAGDALDHSLRTADALPAVDPV
                     LRLAGLLHDLGKATTLADGHFLHHDRDGAVLAEQVLQRLRAPRAEVTRVGRLVRHHMF
                     AYTPDWTDAAVRRFVRRVGVDLLPDLFALREADDVASGVDEPPGGWKELRDRVASVAN
                     DPLEARQLAISGDDLVTQLGIAPGPAIGRMLAALLDAVVEDPTLNSREQLLTLARAMA
                     SG"
     gene            complement(3161..3694)
                     /locus_tag="VIN32_07835"
     CDS             complement(3161..3694)
                     /locus_tag="VIN32_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008474551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEY8922522.1"
                     /translation="MLNAPVLVLNQNYEPLNVCDIRRAFNLLGSEKAELLERNHQVIH
                     TPTLAIPAPSVIRLVYLVRRPRPRVKLSRREVFARDAHTCQYCGASTRDLTIDHVVPK
                     HRGGRHEWENLVAACRSCNHRKGSKTLTEARMLLRREPRAPRADVRYLYGTYLADEQN
                     DAWRSYLFLSDAPGSSA"
     gene            3877..>4011
                     /locus_tag="VIN32_07840"
     CDS             3877..>4011
                     /locus_tag="VIN32_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HEY8922523.1"
                     /translation="METSTWTTKVGLARMLSGGVIMDVVTADHARIAQEAGAVAVMAL
                     E"
BASE COUNT          597 a         1422 c         1421 g          571 t
ORIGIN      
        1 cccatgcatc ggccagcatc tgctcgagct tggaatgctc ggggtgccag ccaagggcga
       61 gccgcgctcg gcgcgacgag gccaccagca cgggcgggtc gccggtgcga cgcttcacga
      121 cgcgggccgg gattgcgcga ccggtcacct tgcgcgccgc ctcgactacc tcgcggacgc
      181 tgaagccggc cgcggaaccg aggttgtaga cctcgaacga ggggtcaccc tcaccggtca
      241 cctcgagcgc gcgcaggtgc gcggcggcga ggtcgcggac gtggatgaag tcgcgaatgc
      301 aggtcccgtc cggggtcggg tagtcctgcc cgaagatctg gacgtgcgtc gcctcgcccg
      361 cggccacccg tagcatgagg ggcatgaggt gcgtctcggg ctcgtggtcc tcgccattgc
      421 gctccgtggc accggcgacg ttgaagtacc ggaggctgat cgcctggaag tcgtggctgg
      481 cggcgaacca gcgcatcgcg ccctcgaagg cgagcttcga ggccccgtat gggttgatcg
      541 gctcggtccg gtccgactcg gcgatcggga ctcggcgcgg ctcgccgtag accgctgcgg
      601 tcgaggagaa gaccagccga ttgacgccgg tgacgcgcat cgcctccagc agcgcaaccc
      661 caccgcccac gttcttgcgg tagtagaggc ccgggtcagc catgctctcg ccgaccagcg
      721 acttggcggc gcagtggagg accgcgtcga tccgctcctc gcgcaggacg tgctccaggt
      781 gcccctggtc ctcgatctgc ccccgcacca tgcgtgcggg tcgcacgacg gcggaggcgt
      841 gcccgctcga caagtcgtca tagaccacga cgtcgtgccc ggcctccacg aacgtctcaa
      901 cgctggtcga gcccacgaac ccggccccac ccacgaccag gatgcgcaat ctgctgcctc
      961 ctcgccgatc cggctcagcg ggccgccgcg ccgacgtcgg cgggcgatcc tgcggccttg
     1021 ggcgccggct gctgctgcca cgccatgaac tcggcggcca gctgcgggct gaacgtgctc
     1081 gagatgcggt ctgctcccgc cgcgacagcc tcgaccaatt gcgacatctc ggtgaactcg
     1141 ccggcggcct tcacctgaag gtggcccccc accacgccgt gcaggaagcg cgcctgttcg
     1201 acggtggcat cggcctgctc ccgcccatcc gaggtgacca cgtacgcggc cgccgagcga
     1261 gccgacagcc ggcaggcgcg ccgaagcagc tcctcgctga cgggccccgc ccgcacgatc
     1321 acgccgcagg tggccatggc ggattgagcc atctcgacca ccgccagcat gtcattgagc
     1381 accgcttcgt cgtccccgga gacgagcgcg cctgcgttga gcatcacgtc gagctgggtt
     1441 gctccgtgct cgagcgcggt ggatgcctcg aacgccttga cggcggcaac ctggccgcca
     1501 tggggaaagc cgatcaccgt gcccaccgcc accggcgtcc gcgagaggag ccgcgcggcc
     1561 ggcttcacca gccaggggct gaccaggagc gtcgccagct ggtgctccag ggcgagaccg
     1621 gcagcgacat gcagatccgc cagggatgcc gtcggccggg tcagccgatg ctcaagccgc
     1681 gccgtgcgcg ccagggcagt catcgcgggg atgggaacct ccgctccagg tcatcggcgc
     1741 gtgggcacgc gctccggatc gccgattgta gcagcgccct atccgctagc catggctcgg
     1801 gcaagtgtca gcagctgctc tcgcgagttg agggtcgggt cctccaccac ggcgtccagc
     1861 agcgcggcca gcattcgccc gatggcggga ccgggcgcga tcccgagctg ggtgaccagg
     1921 tcgtcgccgc tgatcgccag ctgccgggcc tccagcggat cgttggcgac ggaggcaact
     1981 cggtcgcgga gctccttcca tcctccgggc ggctcgtcga cgccggaggc cacgtcatcg
     2041 gcctcgcgca gggcgaagag gtcgggcagc aggtcaacgc ccacgcggcg aacgaagcgc
     2101 cgcacggcag catcggtcca gtcgggggtg taggcgaaca tgtggtggcg caccaggcga
     2161 cccacgcggg tgacctcggc acggggtgcc cgcagccgct gcaggacctg ctcggcgagg
     2221 actgcgccgt cgcggtcgtg atgcaggaaa tgcccgtccg ccagcgtcgt ggccttgccc
     2281 aggtcatgga gcaggccggc caggcgcagc acggggtcca cggccggcag ggcgtcggcc
     2341 gtgcgcaggg aatggtcgag ggcgtccccg gccagtcgct tcgtctgcgg gacgccgcgc
     2401 agggccgcca gctcggcgag cagcacgtcg agcagcccca gccgctccat cagctcgaag
     2461 gcgcgggatg gcggagcggc gcccgccagg atccgcatga gctcgtcacg gacccgctca
     2521 ccggacaggc cggccgcgtt cggcgcgttc gcaaggatcg ccgcctcggt cggcggatcg
     2581 acccggagct ggaagcggtt ggcgagccgg acggcacgga ggagccgcag cgcgtcctcg
     2641 tcgaatcgct catcggggtc gccgacggct cgcagcagcc ctgcctcgag gtccgccgct
     2701 ccaccgtggg ggtcgaccag ccgcccgctg ccatcggctg cggcgtcggg cagccacgcc
     2761 atggcgttga tggtgaaatc gcgccgggcc aggtcgtcgg ccagcgactg gccccaacgc
     2821 acctcgtcgg gccggcgccg gtcccggtag cccccctcga cccggtaggt ggtgacctcg
     2881 atcccccgtc cgccagccgg atcgcggacc gtcaccgtgc caaatgggtt ctcccaggtg
     2941 gcccctggga agcgggcagc caccgactcc gggggtgccg aggttgccac gtcccagtcg
     3001 cccggctggt cgccacgcac caggtcgcga acgctgccac ccaccagcgc cgactccgcg
     3061 cctccctggc gcaacgcgtc gagcacgctg gccacggctg gtggcaggtc gatccgcaga
     3121 ggatccccat cgcgcatcgg ttcctccccg ccggtttggc ctaggcggac gaacccggag
     3181 cgtcgctcag gaagaggtag cttcgccagg cgtcgttctg ctcgtcggcg agataggtgc
     3241 catacaggta gcgcacgtcc gcccgcgggg cacgcggctc gcggcgcagc agcatccgcg
     3301 cctcggtcag cgtcttgctc cccttgcgat ggttgcagga gcggcatgca gcgaccaggt
     3361 tctcccattc gtgccgaccg ccacggtgct tggggacgac gtggtcgatg gtcaggtctc
     3421 gcgtcgacgc gccgcagtac tggcaggtgt gcgcgtcgcg tgcgaacacc tcgcgccggc
     3481 tgagcttgac ccgcgggcga gggcgacgga cgagatagac caggcgaatg accgatggcg
     3541 cggggatcgc cagcgtcggc gtatggatca cctggtggtt gcgctccagc agctcggcct
     3601 tctccgagcc gagcaggttg aaggcccgcc tgatgtcgca gacgttgagg ggttcgtaat
     3661 tctggttaag gaccaggacc ggtgcgttga gcaatccgtg cctccgtggc gcggatgata
     3721 gcaatcgcgt gcggattcgc ttccgagcgt caatgacccg gtggcagatg ggagctcggc
     3781 acgctcgcga gtacaatgca tggccgagcg cggcgcggca gcgccgccat cggtctcaag
     3841 cacgaagttc gctaacgaag aggctgcatg gccgccattg aaaccagcac ctggacaacc
     3901 aaggtcgggc tcgcccgcat gctctccggc ggcgtcatca tggacgtcgt cacggccgat
     3961 cacgcccgga tcgcgcagga ggccggggcg gtcgccgtca tggccctcga g
//
LOCUS       DATCQR010000236         8479 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_91018, whole genome shotgun sequence.
ACCESSION   DATCQR010000236 DATCQR010000000
VERSION     DATCQR010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 8479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8479
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91018"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2335)
                     /locus_tag="VIN32_07845"
     CDS             complement(<1..2335)
                     /locus_tag="VIN32_07845"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEY8922524.1"
                     /translation="MRLATLAWRGLTARPLRTALTIIGVALGVALVSGTLLANQAASE
                     AVERAAREILGKAELRVRAFDPNGFSAREVEIIRRISPAVLEAAAVAERRINTSTVGG
                     PDEKVFAPLLALGVDPDIEQSVRTYDLEAGTFLTPGHPEDILLNAAWASARGLAVGDE
                     LVFTGADPAMPHPRIVGLMGDLGVGALEQGNVLVIDRAFLDDAFKAHAPVTFVDLVVA
                     DGRGDDVEAALNSGMTEPFIVERLADTQIQLSRAQQGFAGIAFLFGLVALVVGSFLVA
                     NTLAMTLSEQTREIGLLRAAGLTGRQVMGIFLRQGAVVAVVGSVVGLAVGIGFAALII
                     GFLRSTRAVLVTGLPINPLALLVAFVMGIAVTLAASAFPAAAAARVSPMDALRPSRQP
                     GRTLWGRLRWIVILELVVVLLGIAAYPVDRGDFGVPGVALALAILLAGTALTALLLQP
                     LSRVVGRPFEWFFGAEGLLGRANLGRDRARTGLTVSALIIALSAVVTLGVVANSARAT
                     AERWVGSVLPGGYAIRYTLPLKIDTQQPELENVAGVAAVTPIADFPAVIREGDKQREA
                     SVAGIDPGVFSATGSLIFVHGARDAAFQALEDGGAILVSEPVASRDNLGIGSTVELAE
                     PGAESRIFTVAGIVAYTLPGRSADGALLISLADARASFGADSAEVWAFVKQPDVPDAA
                     FRSALEQRARDDSAELLTAAGLSSDLERSLDRIIGLFDVLALLAVVIAGLGIVNTLGV
                     SVLERAREIAILRSHGMTTGQVQAMVVAEASIMGAIGA"
     gene            complement(2340..3086)
                     /locus_tag="VIN32_07850"
     CDS             complement(2340..3086)
                     /locus_tag="VIN32_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8922525.1"
                     /translation="MSVILEAHDLVKRYPLGDGSVDALRGVSLSVAKGEFVAIMGSSG
                     SGKSTMLHLLGGLDRPSEGNVLIDGVNISQLNDEEATLTRREKTGFVFQFFNLISLLN
                     VAENVALPFLIAGDSLATHRERVDALLDLVGLSNKAKHRPDQLSAGEQQRVALARALA
                     NEPAILLADEPTGNLDFVTGTEILDLIWDSCDRLGQTIVLVTHDARAAAYADRVLIVR
                     DGTIRDEIDLGRRRDHEARPLITRLAALGL"
     gene            complement(3130..4017)
                     /locus_tag="VIN32_07855"
     CDS             complement(3130..4017)
                     /locus_tag="VIN32_07855"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEY8922526.1"
                     /translation="MFTATPRRQPRRIMAKAIALSGTALLAAALMAWTPQPATGWDQS
                     SAEATLWQLLNGDRTNNGLPPLAKHSTLVSLARWRSKDMVDRDYFNHTILGTAYQIYH
                     WYDSNGLSWSSGGENIGYNNGFSDADSPVKINEGFMASSGHRANILNSAWSHGGVGAY
                     GKDGVMWGGKVRNIRMYTELFIKLKSAAPPPPPPATPPPPPPPPPPTPKPVVATPKPT
                     PVATPRQVPVETPVPTPEPTPKPKVARLLATVHSTVGDDAPGSNASVAHSMRVETAAA
                     PDRGIFETVIGSLLSFFLG"
     gene            complement(4158..4233)
                     /locus_tag="VIN32_07860"
     tRNA            complement(4158..4233)
                     /locus_tag="VIN32_07860"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4198..4200),aa:His,seq:gtg)
     gene            complement(4297..4566)
                     /locus_tag="VIN32_07865"
     CDS             complement(4297..4566)
                     /locus_tag="VIN32_07865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEY8922527.1"
                     /translation="MPTGLDPLGWIAIGFIAGAVAGWFVPDHGRMGCLGTTLLGIVGG
                     LVGGWAWTNLLHQDRAGGFLGALLIAIVGSAFVLLLVRGMSRRQV"
     gene            complement(4577..5044)
                     /locus_tag="VIN32_07870"
     CDS             complement(4577..5044)
                     /locus_tag="VIN32_07870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8922528.1"
                     /translation="MRRSIVAGLLAAAAVGGLLLLVRPFIFSFAPPLSDANYTVASAA
                     AIRNGPALVEIVLNESHDLPGEVHRNEAVGLTVVVSAVGTEAFAVVDAWSPTNDCALT
                     LAADRVVDCAGDTWTFDGVPIDPADPPLLAFPTSVNNGAVVTDFTRPNSPPAS"
     gene            complement(5072..5863)
                     /locus_tag="VIN32_07875"
     CDS             complement(5072..5863)
                     /locus_tag="VIN32_07875"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY8922529.1"
                     /translation="MRPGYVWAVFAKEWRDLLSNRLLLGAVAFPALVFAAIPTGLVAF
                     IQARDLDPNQLGQIQQYIVNFPNVPAKLAAQAFIVTNFMAYFLLIPAMVPMAIATQSV
                     IGEKVARSLEPQLATPLEVSELLTGKTLAAALPAVTATWLVFIGYGLVNGLIADPRLT
                     RFVFSDVWITAMLTLVPLICLLSVLLGIVISSRVNDARTAQQIGGFVVIPIIGIAVAQ
                     FFGGNATFSMQQVLIGDLMVAGLVGVTLLIGSWAFDRESILTRLT"
     gene            complement(5860..6801)
                     /locus_tag="VIN32_07880"
     CDS             complement(5860..6801)
                     /locus_tag="VIN32_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007918259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8922530.1"
                     /translation="MIVVEGLRKDFRTLTAVHDMSFTVGDGEIFGLLGPNGAGKTTTV
                     RMLAGLISPTAGTAIVNGHPLGETTQQIRAFTGILTESPGLHEKLTARQNLAYYGRLY
                     GLTGARLREAVDRYLGVVEMSEHADRRVGGFSKGMRQKVAIARALLHEPEVIYLDEPT
                     SGLDPSAAKTVRDFIATLRGLGRSIVVCTHNLDEAERLCDRIGIMRGTLLRVDTPAGL
                     RRQGRTATVRVELNGARGPESFVGRLAELPYVNGVQARENTLVVELADPPHETPDLVA
                     ELVSAGARITSVVEDAQTLEEAYLALVGEIGERDTDR"
     gene            6888..7424
                     /locus_tag="VIN32_07885"
     CDS             6888..7424
                     /locus_tag="VIN32_07885"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEY8922531.1"
                     /translation="MPERTVSSRRAFTGRLLSLRVDEVELESGRRATREVVEHPGAVA
                     ILAWDGERLAAVRQWRQAAAADLLEIPAGTREPAESPLDSAQRELAEECGLSAARWEE
                     GPSFYTAPGFSTELLALFLARDLEAVDVPPAEDEALERSWLTLADALTAIDDGRIRDA
                     KSLVGILWLSRHLPAERH"
     gene            7427..7942
                     /locus_tag="VIN32_07890"
     CDS             7427..7942
                     /locus_tag="VIN32_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012876273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YetF domain-containing protein"
                     /protein_id="HEY8922532.1"
                     /translation="MWDLQVPAWELAVRATGIYVAVLLGLRLFGKREVGQFTLIDLVL
                     ILLLANAVQPAMTGPDTSLTGGLVIIATLIAVNRAIALARARLPFVQWLVESPPTTLA
                     RNGKWVKGALKREDLSAEDLSMALHEHGLTDVSEVKLAVLEGDGMISVVPLDPAKAGH
                     RRPRTRIVRRL"
     gene            7968..>8479
                     /locus_tag="VIN32_07895"
     CDS             7968..>8479
                     /locus_tag="VIN32_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IB"
                     /protein_id="HEY8922533.1"
                     /translation="MLDPADSAEDAGLRYVSDAAPGIERRRAGRGFTYIGLDGKRLTD
                     RAKLAWIRSLAIPPAWTDVWICSSQNGHLQATGRDARGRKQYRYHPRWRSQRDEVKYD
                     RLLAFGKALPRMRRRVARDLRRSGLPRERVLAAVVWLLEKTGIRVGNEEYARDNKSYG
                     LSTLRGRHAQV"
BASE COUNT         1388 a         2845 c         2993 g         1253 t
ORIGIN      
        1 cagcaccgat ggcgcccatg atcgatgcct ccgccaccac cattgcctgc acctggccgg
       61 tggtcatgcc atgcgagcgc aggatcgcga tctctcgcgc ccgctccagc acgctgaccc
      121 ccagcgtatt gacgatcccc aacccggcga tcaccaccgc cagcagcgca agcacgtcga
      181 agaggccgat gatccggtcc agcgagcgct ccaggtcact cgaaaggccc gccgcggtga
      241 gcagctccgc cgaatcgtcc ctcgcccgtt gttcgagggc agagcggaac gcggcatcgg
      301 ggacgtccgg ctgctttacg aatgcccaaa cctccgcgct gtccgctccg aagcttgctc
      361 gcgcgtcggc caggctgatc aggagggcgc cgtccgccga tcgacccggg agcgtgtagg
      421 cgacgatccc cgcgacggtg aagatccggc tctccgcacc cggctctgcc agctcaacgg
      481 tcgatccgat gccgaggttg tctcgtgagg cgacgggctc cgagacgaga attgcgccgc
      541 catcctccag ggcctggaat gcggcatccc gcgcaccgtg gacgaagatc agcgagccgg
      601 tggcggagaa gacgcccgga tcgatcccgg cgaccgatgc ctcccgctgc ttgtcaccct
      661 cgcgaatcac ggccggaaag tcggcgatcg gggtgacggc tgccaccccg gccacgttct
      721 cgagctccgg ctgctgagtg tcgatcttga gcggcaaggt gtagcgaatc gcatagccgc
      781 cggggagcac cgagccgacc catcgttcgg ccgtggcgcg ggcgctgttg gcgacgacgc
      841 ccagcgtcac caccgcgctc aacgcgatga tcagtgcgct gaccgtcaac cccgtgcgag
      901 ccctgtcgcg tcccaggttc gcccgaccaa gcagcccttc ggcgccgaag aaccattcga
      961 acggcctgcc caccacgcgg ctgagcggct gaaggaggag ggcggtgagc gccgtcccgg
     1021 cgagcaggat cgcgagcgcc agtgcgacgc cgggaacgcc gaagtcgcct cgatcgaccg
     1081 ggtatgccgc aattcccagc aggaccacca ccagctccag gatcacgatc cagcgcaggc
     1141 gaccccacag ggttcggccc ggctgccgcg agggacgcag ggcgtccatg ggcgagacgc
     1201 gcgcggccgc tgcagcgggg aacgccgaag ccgcgagcgt gaccgcgatg cccatcacga
     1261 aggcgaccag gagggcgagc gggttgatcg gcaggcccgt caccagcacc gcccgggtgc
     1321 tgcgcaggaa cccgatgatc aaggcggcga agccgatgcc gacggccagg cccaccacgc
     1381 tgccgacgac cgcaaccacg gccccctgcc gcaggaagat gcccatcacc tggcgaccag
     1441 tcagcccggc ggcccgcaac aggccgatct cgcgcgtctg ctcgctgagc gtcatggcca
     1501 gggtgttggc gaccaggaac gagccgacca ccagcgccac cagcccgaac aggaatgcga
     1561 tcccggcgaa gccctgctgg gcccggctca gctggatctg ggtatccgcc agccgctcga
     1621 cgatgaacgg ctcggtcatg ccgctgttca gggccgcctc gacgtcgtcg ccacggccgt
     1681 ccgccacgac caggtccacg aaggtcaccg gcgcatgtgc cttgaacgcg tcgtcaagga
     1741 aggcacggtc gataacgagc acgttcccct gctccagtgc ccctaccccc aggtcgccca
     1801 tcaaccccac gatgcgaggg tgcggcatgg ccggatccgc cccggtgaag accagctcat
     1861 ccccgaccgc caggccgcgc gcgctcgccc aggcggcgtt caggagaatg tcctcggggt
     1921 ggccgggggt gaggaaggtg cctgcttcaa ggtcgtaggt tcggacagac tgctcaatgt
     1981 ctggatcaac accgagggcg agaagggggg cgaagacctt ctcgtcgggg ccgccgacgg
     2041 ttgaggtgtt gatgcgccgc tcggcgactg ctgccgcctc gaggacggcc ggggaaatgc
     2101 gccggatgat ctcgacctcg cgggcactga agccgttggg gtcgaacgcg cgcacccgca
     2161 gctcggcctt gcccaggatc tcccgggcgg ctcgctcgac ggcctcgctg gcggcctggt
     2221 tggcgaggag cgtccccgac acgagggcca ctccaagggc gaccccgatg atggtcagcg
     2281 cggtccgcag gggccgcgcg gtcaggccac gccaggcgag ggtcgccagg cgcatcggcc
     2341 tagaggccca gcgcggccag acgggtgatc agcggccgtg cctcatggtc ccgacgccgg
     2401 cccaggtcga tctcgtcgcg aatcgtgccg tcccgcacga tcaggaccct gtcggcgtag
     2461 gccgcggccc tcgcgtcgtg cgtgaccagc acgatcgtct gccccagacg gtcgcacgag
     2521 tcccagatca ggtcgaggat ctcggtgccg gtcacgaagt cgaggttgcc ggtgggctca
     2581 tcggccagga ggatcgccgg ttcgttggcg agcgcccgag ccagtgccac gcgctgctgc
     2641 tcgccggcgg agagctggtc cggtcggtgc ttcgccttgt tggagagccc caccaggtcc
     2701 agcagcgcat ccacgcgctc gcgatgcgtc gccagcgagt ctccggcgat caggaatggc
     2761 agcgctacgt tctcggcgac gttcaggagc gagatgaggt tgaagaactg aaagacgaac
     2821 ccggtcttct cgcgacgggt gagggtcgcc tcttcgtcgt tcagctggct gatgttcacg
     2881 ccgtcgatca ggacgttccc ttcggagggc cgatcgaggc cgccgagcag gtgaagcatc
     2941 gtcgacttgc cggagcccga cgatcccatg atcgccacga actcgccctt ggcgaccgag
     3001 agggagacgc ctcgcagagc gtcgaccgag ccatcgccga gcggatagcg cttgacgagg
     3061 tcgtgcgctt cgagaatcac cgacatcgcc cgcagtctag cccggcggcc ggagccgctg
     3121 caggcaccct cagcccagga agaagctgag caatgagccg atcacggtct cgaagatgcc
     3181 gcgatcgggg gctgcggctg tctcgacccg catgctgtgg gccacgcttg cattggatcc
     3241 cggcgcgtcg tcaccgaccg tggagtgcac ggtggccaac aggcgggcca ccttcggctt
     3301 cggcgtcggt tccggggttg ggaccggcgt ctcgaccggc acctgtcgcg gcgtggcgac
     3361 cggagtcggc ttcggggtcg cgacgactgg cttgggggtc ggcgggggtg gcggtggtgg
     3421 cggaggcggc gtcgcgggtg gcggcggagg cggcgctgcc gacttgagct tgatgaagag
     3481 ctcggtgtac atccggatgt tgcggacctt gccgccccac atcacgccgt ccttgccgta
     3541 ggcgccaacg cctccgtggc tccaggcgga gttcaggatg ttggcccggt gtcccgagga
     3601 ggccatgaac ccttcgttga tcttcaccgg cgagtcggca tcggagaagc cgttgttgta
     3661 gccgatgttc tcgccgcccg atgaccagct caggccgttg ctgtcgtacc agtgatagat
     3721 ctggtaggcg gtaccgagga tagtgtggtt gaagtagtca cgatcgacca tgtccttgct
     3781 gcgccagcgc gccaggctca ccagcgtgct gtgcttcgcg agcggcggaa ggccgttgtt
     3841 ggtgcggtca ccattcagca gctgccacag cgtggcctcg gctgaggact ggtcccaacc
     3901 ggtggcgggc tggggggtcc aggccatcag cgcagctgcc aggagggcgg ttccggagag
     3961 ggcgatggcc ttcgccatga tgcggcgtgg ctgtcgtcgc ggcgtcgcgg tgaacatgca
     4021 aggagcctag gcggcgagct gggggagggt ctgtagcgca catgtcccgg gggcgtggca
     4081 tgacctatgg gccgcccggg tacccccgca cgtaggacca tgggtggtgc ggacgggcaa
     4141 cgcccccgtt tatcgggtgg ggtggcctac gggggtcgaa cccgtaacct tcggatccac
     4201 aatccgatgc tctgccggtt gagctaaggc caccacggaa cgcacgcgaa atggggcggg
     4261 agcgggccgg attctcccac gcactcgaga ggagcgtcaa acctggcggc ggctcattcc
     4321 ccggaccagg aggagcacga aggccgagcc cacgatggcg atcaggagag cgccgaggaa
     4381 gccgccggcc cgatcctggt gcaggaggtt ggtccacgcc caacccccga ccaggccgcc
     4441 aacgatcccg agcagcgtcg tgccgagaca gcccatccgg ccgtgatcgg ggacgaacca
     4501 gcccgccacg gcgccggcga tgaatccaat cgcgatccat ccgagggggt cgagtccggt
     4561 gggcatgtcg cgaatcctac gatgccggag gcgagttcgg gcgggtgaaa tccgtgacga
     4621 ctgcgccgtt gttgacgctg gtggggaagg caagcagcgg tggatcggct gggtcgatcg
     4681 ggacgccgtc gaaggtccag gtgtcccccg cacagtcgac cacccggtca gcggcgagcg
     4741 tcagcgcaca gtcgttggtg gggctccagg catcgaccac ggcgaacgcc tccgtcccga
     4801 cggccgagac cacgacggtc aggcccacgg cctcgtttcg gtgcacctcg ccggggaggt
     4861 cgtgggactc gttcaggacg atctcgacca gcgccgggcc atttcgaatt gcggccgcgg
     4921 atgccacggt gtagttggcg tcgctcagcg gcggcgcgaa gctgaagatg aacgggcgca
     4981 ccaatagcag caggccgccc acggcggctg cggccagcag gccggccacg atggaccgac
     5041 gcacgggaag cggatcgcgg cgatccatcc ctcaggtgag gcgggtcagg attgactcgc
     5101 gatcgaacgc ccatgaaccg atcagcaggg tcactcccac caggcccgcg accatcaggt
     5161 cgccgatcag gacctgctgc atcgaaaagg tcgcgttccc gccgaagaac tgggccacgg
     5221 cgatgccgat gatcggaatc accacgaatc ctccgatctg ctgggcggtc cgggcgtcgt
     5281 tgacgcggct gctgatcacg atccccagca ggacgctgag gaggcagatg agcggcacca
     5341 gcgtgagcat ggcggtgatc cacacgtcgc tgaacacgaa ccgcgtcagc cgcgggtcgg
     5401 cgatgaggcc gttgaccagg ccatagccga tgaagaccag ccaggtggcg gtaaccgccg
     5461 gcagcgcggc ggcaagcgtc ttcccggtga gcagctccga cacctcgagc ggcgtcgcca
     5521 gctgtggctc gagcgagcgc gccaccttct cgccgatcac cgactgggtc gcgatcgcca
     5581 tcggcaccat ggccgggatg agcaggaagt aggccatgaa gttggtgacg atgaaggcct
     5641 gggcggccag cttggcgggg acgttcggga agttgacgat gtactgctgg atctgaccca
     5701 gctggttcgg gtccaggtca cgagcctgga tgaaggcgac cagcccggtg gggatggcgg
     5761 cgaagacgag ggccgggaag gcgacggcgc cgaggagcag ccgattgctg agcaggtcgc
     5821 gccactcctt ggcgaacacg gcccagacgt agccaggcct catcggtcgg tgtcccgttc
     5881 gccgatctcg cccacgagtg cgaggtaggc ctcttccagc gtctgcgcgt cctcgacgac
     5941 agaggtgatg cgggcaccgg cgctcaccag ctcggcgacc aggtcaggcg tctcgtgggg
     6001 cgggtcggcc agctccacga ccagcgtgtt ctccctggcc tgcacgccgt tgacgtacgg
     6061 cagctcggcc aggcggccga cgaacgattc cggtccacgg gccccattca gctcgacgcg
     6121 gaccgtcgcc gtccgccctt gccgccgcag gccggccggg gtgtccacgc gcagcagggt
     6181 gccgcgcatg atcccgatcc gatcgcagag ccgctccgcc tcgtcgaggt tgtgggtgca
     6241 caccacgatc gagcggccca ggccacggag cgtggcgatg aagtcgcgaa ctgtcttggc
     6301 cgccgacggg tcgaggccac ttgtcggctc gtcgaggtag atcacctccg gctcgtgcag
     6361 cagcgcccgg gcgatggcca ccttctggcg cattcccttg ctgaagccgc cgaccctgcg
     6421 gtcggcatgc tcgctcatct cgaccacgcc gaggtatcgg tccaccgcct cgcgcagtcg
     6481 ggccccagtc aggccataca ggcggccgta gtacgccagg ttctggcgcg ccgtcagctt
     6541 ctcgtggagg ccggggctct cggtcaggat gccggtgaag gcgcggatct gctgggtcgt
     6601 ctccccgagc ggatggccgt tgacgatggc cgtgccggcg gtcggtgaga tcagccccgc
     6661 cagcatgcgg acggtggtcg tcttgccggc gccgttcggg ccgagcaggc cgaagatctc
     6721 gccatcgccc acggtgaagg acatgtcgtg caccgcggtg agcgtgcgga aatccttgcg
     6781 aagcccctcg acgacgatca tcggcgctga tgctagcggc tggaggctcc ctccactgcc
     6841 ggacgaatga tgagtctccc gcctgaccaa cgggtaggat gcgggagatg ccagagcgaa
     6901 ccgtgagcag ccgtcgcgca ttcaccgggc gcctcctgtc gctgcgcgtg gacgaggtcg
     6961 agctggaatc gggtaggcgc gcaacccggg aggtggtcga gcatcccgga gcggtggcga
     7021 tcctggcgtg ggacggcgaa cggctggcgg cggtgcgcca gtggcgccag gcggcggcgg
     7081 ccgacctgct cgagatcccg gccggcaccc gcgagccggc cgagtcaccc cttgactctg
     7141 cgcaacgcga gctggccgag gagtgtggcc tgagcgccgc gcgctgggag gaggggcctt
     7201 ccttctatac cgccccgggc ttctccaccg agctgctcgc cctcttcctg gcaagagatc
     7261 tcgaggccgt ggacgtgcca ccggccgagg acgaggcgct cgagcgatcg tggctgacgc
     7321 tggccgacgc gctgaccgcg atcgacgacg ggcgcatccg cgacgcgaag tcgctggttg
     7381 gcatcctctg gctctccagg cacctccccg cggagcggca ctgacgatgt gggacctgca
     7441 ggtcccggcc tgggagctgg ccgtccgcgc cactggcatc tatgttgccg tcctgctcgg
     7501 cctgcgcctc tttgggaagc gagaggtggg ccagttcacc ctgatcgacc tcgtcctgat
     7561 cctgctgctg gcgaacgcgg tgcagccggc gatgaccgga cccgacacct ccctgaccgg
     7621 cgggctggtc atcatcgcga ccctcatcgc ggtcaaccgc gccattgcgc tggcgcgggc
     7681 caggctgcca ttcgtgcagt ggctcgtcga gtcgcctccg accacgctgg cgcggaacgg
     7741 caagtgggtc aaaggggcgt tgaagcgcga ggacctctcg gcagaagacc tgagcatggc
     7801 cctgcacgag cacggattga cagacgtgtc cgaagtcaag ctggcggtgc tcgaagggga
     7861 cgggatgatc agcgtcgtgc cactggatcc tgccaaggcg gggcatcggc gcccccgcac
     7921 gcgaatcgtg cgccggctct agcgggagct cggcgagcag cgcggccatg ctggatccag
     7981 ccgactcggc ggaggacgcc ggcctgcgtt acgtctcgga cgccgcgccg ggcatcgaac
     8041 ggaggcgagc gggtcgcgga ttcacctata tcggtctgga tggcaagcga ctgacggacc
     8101 gtgccaagct ggcgtggatt cgaagtctgg ccattccccc agcgtggacc gacgtctgga
     8161 tctgcagctc gcaaaacggg cacctgcagg ccaccggcag ggacgcgcgg ggccggaagc
     8221 agtaccgcta ccatccccgc tggcggtccc agcgcgacga ggtcaagtac gaccgcctgc
     8281 tcgccttcgg caaggctctg ccccgcatgc gcaggcgggt cgctcgggac ctgcgccgat
     8341 ccggactgcc cagggagcga gtcctcgccg ccgtcgtgtg gttgctggag aagacgggaa
     8401 ttcgcgtcgg caacgaggag tacgcccgcg acaacaagtc gtatggcctg agcaccctcc
     8461 gcggccgcca cgcccaggt
//
LOCUS       DATCQR010000237         2417 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_214977, whole genome shotgun sequence.
ACCESSION   DATCQR010000237 DATCQR010000000
VERSION     DATCQR010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2417
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214977"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..856)
                     /locus_tag="VIN32_07900"
     CDS             complement(<1..856)
                     /locus_tag="VIN32_07900"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF013296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HEY8922534.1"
                     /translation="MSVASAHDLLAGLDGIARIQGTRLLIDDESRLRGEAMDGLVEDA
                     VFNADEAVRDAARWLIWSASQALGCGSASIHELYMARGRGEFAATRFTVPAINVRAVT
                     YLTARQAFSAAMERDAGAVILEIAKSEMAYTDQRPAEYAAVILGAAIRSGWVGPVFLQ
                     GDHFQFNAAKWAADPGAEMSGLKQLTSEAVAAGFCNIDIDSSTLVDLSQPTAAAEQLV
                     NVANTVELTALIRAIEPDGVTISIGGEIGEVGKSNSTEEELRAYLDGYFGMLHGTGMP
                     AISKVSIQT"
     gene            complement(866..1132)
                     /locus_tag="VIN32_07905"
     CDS             complement(866..1132)
                     /locus_tag="VIN32_07905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8922535.1"
                     /translation="MELASPCPPGGPLALGDCVALRPFTLGVLGLGAALYVGGLSAVL
                     AWVSGLRRRGVADGIAARDWYLLAAAVGLPIASLLAFTLLSALR"
     gene            complement(1218..1799)
                     /locus_tag="VIN32_07910"
     CDS             complement(1218..1799)
                     /locus_tag="VIN32_07910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8922536.1"
                     /translation="MSTAYSVPPRRDLISRATHEIDDFMSWLLYGSETWLVALLKGVP
                     LFLFVYFVLGYIPNYANTITTLYLGFNKDVGFLVAVVLIGGPTFTLLLILALWTQAAR
                     GRRGFAWSLIRFLDFLQYLALVLLIIPFMLFNLAGGSLIPLVFPFQALALGAIAAGGG
                     AMSLAYLYFEYRRITRREAEAAAAAAAAWRSGG"
     gene            complement(1796..>2417)
                     /locus_tag="VIN32_07915"
     CDS             complement(1796..>2417)
                     /locus_tag="VIN32_07915"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEY8922537.1"
                     /translation="GDTGRLAPNLHSKSIYDKLKAQNGGSFTDVRKAGDPPDYVNLVI
                     RFGGVVVSGDVNSPMPAWSTEVSGPLTVNQIDALTALVETWALEAGSQPDQAVPDTVE
                     AGQKVFVDAACGGCHGADLSGAIGPSLLNIGNAPVTDLPTPISQLDKLKADYAADSRT
                     FLERWIRDSAVNYNDGTATGMPVHPVGTISDSAMQALITFLLSQKQ"
BASE COUNT          415 a          784 c          856 g          362 t
ORIGIN      
        1 cggtctggat gctcaccttg ctgatcgctg gcattcccgt gccgtgcagc atcccgaagt
       61 agccatccag gtaggcccgc agctcctcct cggtggagtt gctcttcccc acctcgccga
      121 tctcgccgcc gatgctgatc gtcacgccgt ctggctcgat ggcgcgaatg agcgccgtca
      181 gttccaccgt gttggcaacg ttgacgagct gctcggcggc ggcagtcggc tgggagaggt
      241 cgacgagggt gctgctgtcg atgtcgatgt tgcagaagcc ggccgccacc gcctcgctgg
      301 tgagctgctt gaggccggac atctcggcgc ctgggtcagc cgcccacttg gcggcgttga
      361 actggaagtg gtcgccctgc aggaagacgg gaccgaccca gccagaccga atggcggcgc
      421 cgaggatgac ggcggcgtac tcggcgggac gctggtcggt gtatgccatc tcggacttgg
      481 cgatctcgag gatgaccgcc ccagcgtcgc gctccatggc ggccgagaac gcctgccgcg
      541 ccgtgaggta tgtaacggcc cgaacgttga tcgccggcac cgtgaacctg gtggcggcga
      601 actccccgcg cccgcgagcc atgtacagct cgtggatgct ggccgatcca cagcccaggg
      661 cctgcgacgc gctccagatc agccagcgcg ccgcgtcgcg aacagcctca tcggcattga
      721 agaccgcatc ctccaccagt ccgtccatcg cctcgccgcg gaggcgcgac tcgtcgtcga
      781 taagcagacg ggtcccctgg atgcgggcga tcccgtccag gcccgcaagc aggtcgtgcg
      841 cgctggccac gctcatgcgg ccatgctagc ggagcgccga gaggagggtg aacgcgagga
      901 gggacgcgat cgggaggccc actgcggcgg ccagcaggta ccagtctcga gcggcgatcc
      961 cgtccgccac cccgcgtcgc cgaaggccgc taacccaggc gaggaccgcc gacaggccac
     1021 cgacgtacag ggcggcgccc aggcccagca ccccgagcgt gaagggccga agggcgacgc
     1081 agtcccccag ggccagcggt ccgccgggtg ggcagggcga cgcgagctcc atggcaatca
     1141 gcgcaccggc cccgccaagg atgaccagcg accaggtgag atgagcgagg acgagccgcg
     1201 agatgacgtt gggcggacta gccgccggat ctccaggcgg cagcggctgc cgcggcggcc
     1261 tcggcctcgc ggcgggtgat gcggcggtat tcgaagtaca ggtaggccag gctcattgct
     1321 cctcccccgg cggcgatggc gcccagcgcc agggcctgga acgggaagac gagcgggatc
     1381 aggctcccac cggcgaggtt gaagagcatg aaggggatga tcagcagcac cagcgcgagg
     1441 tactgcagga agtccaggaa tcggatcagc gaccacgcga agccgcgacg gccgcgagcg
     1501 gcctgggtcc acagggccag gatcaggagc agcgtgaagg ttggcccgcc gatcagcacc
     1561 accgcgacca ggaatccaac gtccttattg aagcccaggt acagggtggt gatggtgttg
     1621 gcgtaattgg ggatgtagcc gagcacgaag tagacgaaga ggaagagcgg cacgcccttg
     1681 agcagggcga cgagccaggt ttcgctgccg tagagcagcc agctcatgaa gtcatcgatc
     1741 tcgtgcgtgg cgcgggagat caggtcgcgc cgcggcggga ccgagtaggc ggtgctcatt
     1801 gcttctggct cagcaggaag gtgatcagcg cctgcatcgc ggagtcgctg attgttccca
     1861 ccggatgcac tggcatgccg gtcgcggtcc cgtcgttgta gttcaccgcc gagtcgcgga
     1921 tccagcgctc gaggaaggtg cgcgaatcag ccgcgtagtc cgccttcagc ttgtcaagct
     1981 gactgatcgg ggtcggcagg tcggtgaccg gcgcgttgcc gatattcagc aggctcgggc
     2041 cgatggcacc agagagatcg gcgccgtggc atccgccaca cgcagcgtcg acgaagacct
     2101 tctggcccgc ctcgaccgtg tcgggaacag cctggtccgg ctgggagcct gcctccagcg
     2161 cccacgtctc gaccagcgcg gtcagggcat cgatctggtt gacggtcagc ggcccgctga
     2221 cctcggtgct ccaggcgggc atcggcgaat tcacgtcgcc ggagacgacc accccaccga
     2281 agcggatcac gaggttcacg tagtcaggtg ggtcgcctgc cttgcgaacg tcggtgaacg
     2341 agcccccgtt ctgggccttg agcttgtcgt agatgctctt cgagtggagg ttgggggcaa
     2401 gccggccggt gtccccc
//
LOCUS       DATCQR010000238         3888 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_105783, whole genome shotgun sequence.
ACCESSION   DATCQR010000238 DATCQR010000000
VERSION     DATCQR010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3888
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105783"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="VIN32_07920"
     CDS             complement(<1..210)
                     /locus_tag="VIN32_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEY8922538.1"
                     /translation="MAGERSDALLQIDVVTLFPELFDVPLHTSVIGRAVERGILSVAV
                     HDLRAHGIGRHRVVDDAPYGGGAGMV"
     gene            complement(210..719)
                     /gene="rimM"
                     /locus_tag="VIN32_07925"
     CDS             complement(210..719)
                     /gene="rimM"
                     /locus_tag="VIN32_07925"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEY8922539.1"
                     /translation="MSWRSSATTERLAVARVLGAKGLAGSLRIEALTDRPERLAAGAS
                     IWIEDETASRTILEAGRGGRVPVLRLEGISDRAAAERLAGRYLEAPAEPLPPDTYYWH
                     QLIGLAAFDESGTELGHVVEIFRAGENEVYRIEGPAGELLVPALRDVVRSIDLGSGRM
                     VVRYDAEEV"
     gene            complement(691..927)
                     /locus_tag="VIN32_07930"
     CDS             complement(691..927)
                     /locus_tag="VIN32_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007923523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEY8922540.1"
                     /translation="MKAFLEYVARSLVDDPDAVTVEMSEADDEVTLTLKVGEGDMGRI
                     IGRDGRIANAIRSLLRVMGARDGRHVELEIVGDD"
     gene            complement(924..1445)
                     /gene="rpsP"
                     /locus_tag="VIN32_07935"
     CDS             complement(924..1445)
                     /gene="rpsP"
                     /locus_tag="VIN32_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011281575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEY8922541.1"
                     /translation="MAVRIRLSRVGATKRPSYRVVVIDSRRPRDGRALEILGYYDPLT
                     EPATVKIDAERLAAWVGKGALPSDTVAKLIKLNGSAPSAAGTPAADSPAPRPKRSRKK
                     AVAAAKAEAGPAEEAPAVDEAPAAEEAPAVDEAPAPDEPAPTGDADTASEAEAAAEPA
                     PAAEAAPAEEASK"
     gene            complement(1506..3059)
                     /locus_tag="VIN32_07940"
     CDS             complement(1506..3059)
                     /locus_tag="VIN32_07940"
                     /inference="COORDINATES: protein motif:HMM:NF023260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3352 domain-containing protein"
                     /protein_id="HEY8922542.1"
                     /translation="MRRVVVAITALLVVLGGAVVVGYLLLFSAVADRAARATPADTAL
                     YVNAYLQPSAGQQMNLFGLVGHLRGFGDPASLEAKIDEVAGRLLGQAAIDYTADVRPW
                     LGTQIAVGFAPGEGGSPPTLLLFAAVKDSAEARRAVPRLFGAMDITFMAETFRGRKVM
                     LSDSTSYALLDDLLVVASTPERLRASLEADADIAPSLADSAAFIAGMGTVASDHLASV
                     YLDMPRAASLPEGQLLGGFGTAVLALTADADALHLHGTAAFTASGASKEARAAFALGT
                     QSSTLAGWMPRTSAAEVVLFGAAQSFEDLEASLAGNSGFGRAIDALNQLRAIAALGLG
                     ISFNHDLLPLFDGDGAVALQRLDADGFHGPVLLRPSDGSAAQASLDRMRSALADRGSK
                     VTTRSVAGTTLTSVSVPQIGTVAYALLDGVVIVAVDAADVAAALEAHAAGETLASDDR
                     YRSAFELTGAHAGNQLWADIPTLVDVLAGFFDPGSELRDILHQIGELAIIATATDDRL
                     EIDGALTVK"
     gene            complement(3065..>3888)
                     /locus_tag="VIN32_07945"
     CDS             complement(3065..>3888)
                     /locus_tag="VIN32_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEY8922543.1"
                     /translation="GMEQARQRGLDVVLLDTAGRLHVDEPMMDELVRVAGAMTPSETL
                     LVVDAMTGQDAVKVAEAFHHRLPLTALVLTKMDGDARGGAALSIRSVTGLPIAFIGTG
                     ERTDGLEPFHPDRLAQRILGMGDVLSLVERAQETVDQHEAERLAQRLMESRFTLDDFR
                     SQLAQVKKMGPIGQLVGMIPGAGQLAGAAQQAVDDGQLKRIEAIIDSMTPGERRHPEL
                     IKASRRRRIATGSGTTPADVNRLLKQFGEMQRLMRQLGSGRLPRTGSGLRGILGG"
BASE COUNT          585 a         1374 c         1328 g          601 t
ORIGIN      
        1 caccatcccc gctcccccgc cgtagggagc gtcgtcgacg actcggtggc gcccgatgcc
       61 gtgcgcgcga aggtcgtgca ccgccactga caggatgccg cgctcgaccg cccgcccgat
      121 caccgatgta tggaggggca cgtcaaacag ctcggggaag agcgtgacaa catcgatctg
      181 gaggagggcg tcggaccgct cgccggccat cagacctcct ctgcgtcgta tcgaacgacc
      241 atccggccgg acccgagatc gatagaccga accacgtcgc ggagcgcggg cacgagcagc
      301 tcgcccgcag gtccctcgat gcggtacacc tcgttctcgc ccgccctgaa gatctcgacc
      361 acgtggccca gctcggtgcc tgactcgtcg aacgccgcca gcccgatcag ctgatgccaa
      421 tagtaggtgt ccggcgggag gggctcagcc ggcgcctcca ggtagcggcc cgccagtcgt
      481 tctgctgcgg cgcgatccga gatgccctcg agccgaagca ccgggacgcg gccgccccgg
      541 ccggcctcca ggatcgtgcg cgaggcggtc tcgtcctcga tccagatcga cgctccggcc
      601 gccaggcgct caggacgatc tgtgagagct tcgatgcgaa gcgagccggc aaggcccttg
      661 gcgccgagca cgcgggcaac cgcgaggcgc tcagtcgtcg ccgacgatct ccagctcaac
      721 gtgtcgcccg tcgcgcgcgc ccatcactcg cagcaggctt cgaatcgcat tcgcgatgcg
      781 gccgtcgcgg ccgatgatgc ggcccatgtc gccctccccc accttgaggg tcagcgtgac
      841 ctcgtcatcg gcctcgctca tctcgacggt gacggcgtct gggtcgtcga caagggagcg
      901 agcgacatac tcgagaaagg ccttcacttt gaggcctctt cggccggtgc agcttcggcc
      961 gctggagcgg gctcggccgc agcctccgct tcagacgctg tgtcagcgtc gcccgtcggg
     1021 gctggctcgt caggcgcggg cgcttcgtcc accgcaggag cctcttccgc tgcgggcgct
     1081 tcgtccaccg caggagcctc ttccgccggc ccagcctcgg ccttggccgc agcgactgcc
     1141 ttcttgcgag aacgctttgg ccggggggct ggtgaatcgg ctgcgggcgt tccggcggct
     1201 gacggcgcac tgccgttcag cttgatcagc ttcgccacgg tgtcggaggg cagtgcccct
     1261 ttgccgaccc acgcggccag cctctcggcg tcgatcttga cggtggccgg ctcggtcaac
     1321 gggtcgtagt acccgaggat ttcgagcgcc cggccatctc gcgggcgacg gctgtcgatg
     1381 accaccaccc gatagctcgg ccgcttggtt gctcccacgc gtgagagcct aatgcgtact
     1441 gccaaaggtt ctctacctct ctgtcgttgc gacggcagtc ggctgccgat gcgaagtctc
     1501 tgatctcatt tgacggtcag cgcgccatcg atctccagcc gatcgtcggt ggcggttgcg
     1561 atgattgcaa gctcgccgat ctggtggagg atatcgcgaa gttcgcttcc tgggtcgaag
     1621 aagccggcta acacatcgac cagggtcgga atgtcggccc acaattggtt gccggcgtgc
     1681 gcgcccgtga gctcaaacgc cgagcggtag cgatcgtcgc tggccagcgt ctcgccggcg
     1741 gcatgcgctt ccagcgctgc cgcgacgtca gccgcatcga cggccacgat gacgacgcca
     1801 tccagcaggg cgtaggccac ggtgccgatc tgcgggaccg agacgctggt gagcgtggtg
     1861 cccgccacac tgcgggtggt caccttcgac ccgcgatcgg cgagcgcaga tcgcatccga
     1921 tccagggacg cctgggcggc cgagccatcg gacgggcgaa gcaggaccgg cccgtggaag
     1981 ccgtcggcgt cgaggcgctg cagggccacc gccccgtcgc cgtcgaagag cggcagcagg
     2041 tcgtggttga agctgatccc gagtccgaga gctgcgatgg cgcgcagctg gttcagcgcg
     2101 tcgattgccc ggccgaagcc cgagttgccg gccaggctcg cctccaggtc ctcgaagctc
     2161 tgcgcggcgc cgaagaggac cacctcggcg gccgaggtgc gcggcatcca tccggcgagc
     2221 gtagacgact gcgtgccgag cgcgaatgcg gcacgcgcct ccttgctcgc gccgctcgcc
     2281 gtgaacgcgg ccgtcccgtg caggtgcagg gcgtcggcat cggccgtgag cgccaggact
     2341 gccgtgccga agccacccag cagttgcccc tccggcaggc ttgcagctcg gggcatgtcg
     2401 aggtagaccg aggcaaggtg atcgctggca acggtgccca tgccggcgat gaacgccgcg
     2461 gagtcggcca gggatggagc gatgtccgca tcggcctcga gggaggcgcg gagccgctcc
     2521 ggcgtgctgg ccaccaccag caggtcgtca agcagggcgt agctcgtcga gtcgctcaac
     2581 atcaccttcc ggccacggaa cgtctccgcc atgaaggtga tgtccatcgc gccgaacagg
     2641 cgggggacgg ccctgcgcgc ttcagcggaa tccttcaccg cggcaaacag cagcagagtt
     2701 ggtggtgagc cgccctcgcc aggcgcgaag cccaccgcga tctgcgtccc cagccagggc
     2761 cgcacgtcgg ccgtgtagtc gatcgccgcc tggccgagca gccggccggc gacctcgtcg
     2821 atcttggctt cgagggaggc cgggtcgcca aagccgcgga ggtgaccgac gagcccgaac
     2881 aggttcatct gctggccggc cgatggctgg aggtaggcgt tgacgtacag cgccgtgtcg
     2941 gccggagtgg cccgcgcagc gcggtccgcg accgctgaga acaggagcag atagccgacc
     3001 accacggccc caccaagcac aacgagcagc gcggtgatcg cgacgacgac tcgccgcatg
     3061 ttcgctagcc gcccaggatg ccgcgcagcc cgctcccggt ccgcggcagt cgacccgatc
     3121 cgagctggcg catcaggcgc tgcatctccc cgaattgctt gagcagccgg ttgacgtcgg
     3181 cgggggtcgt cccgctgccc gtggcgatcc ggcggcggcg gctcgctttg atcagctccg
     3241 gatgccggcg ttccccgggc gtcatcgagt cgatgatggc ctcgatgcgt ttgagctgac
     3301 cgtcgtcgac cgcctgctgg gccgcaccgg ccagctggcc agcgccgggg atcatgccga
     3361 ccagctgacc gatcggtccc atcttcttca cctgcgccag ctgcgaccgg aagtcgtcga
     3421 gcgtgaatcg agactccatc aggcgctgcg ccagccgctc cgcctcgtgc tggtcgaccg
     3481 tctcctgtgc acgctcgacg agcgagagga cgtcgcccat ccccaggatg cgctgcgcga
     3541 ggcggtccgg gtggaagggc tcgaggccgt cggtgcgctc cccggtcccg atgaaggcga
     3601 ttggcaggcc ggtgacgctc cggatgctca gcgcggcacc accccgggcg tcgccatcca
     3661 tcttggtcag caccagcgcg gtcagcggaa gccgatggtg gaaggcctcg gccaccttca
     3721 ccgcgtcctg gccggtcatc gcgtcgacca ccagcagcgt ctccgatggc gtcatggcgc
     3781 cggcgactcg caccagctcg tccatcatcg gctcgtccac gtgcaggcgg cccgcggtgt
     3841 cgagcaggac gacgtccagg ccccgttggc gagcctgctc catccccg
//
LOCUS       DATCQR010000239         8297 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_105785, whole genome shotgun sequence.
ACCESSION   DATCQR010000239 DATCQR010000000
VERSION     DATCQR010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 8297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8297
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105785"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1555
                     /locus_tag="VIN32_07950"
     CDS             <1..1555
                     /locus_tag="VIN32_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HEY8922544.1"
                     /translation="SRSDLPSLRKDGPPFPRLLVANRGEIAVRIIRACRDLGIESIAA
                     YSDADADALHTRMADRTERIGPASAAESYLSIDAVMAAAVRSGAAAIHPGYGFLSENA
                     VFARAVEDAGLVFVGPPPETLASLGDKLAARRSVAAAGVPIVPGLLVPLVADGDGSLA
                     KVDLAGVSFPALLKAAAGGGGRGMRQVADRRELEAALPAAGREALAAFGDGTLYLEQL
                     VSPARHVEVQLLGDRHGNLAVLGERECSVQRRHQKLVEESPSPAVGPALRAELAESAR
                     RVAGTVAFHNAATVEFLVDTDGAHYFLEMNTRLQVEHGVTELVTGLDLVAWQIRVAAG
                     EQLSESVLASTPRGHAIEVRIYAEDPHRRFAPVSGTVTTWRMPAGPGVRLDAGVTGDT
                     PLPLEYDPLLAKLLVQGEDRPAAVARLRRALDEIAIGGVQTDLSFLRWLVDEPAFVTG
                     AYDTGLIPNDWGDGPALTDLERSLAAEVARAARASSSPGPAAVAPVEAESAWQQVARR
                     EAVGRRRPR"
     gene            1552..2016
                     /locus_tag="VIN32_07955"
     CDS             1552..2016
                     /locus_tag="VIN32_07955"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEY8922545.1"
                     /translation="MSDYELSVDGNPVQPATGWALTWLDAEGGVGRLTDGSRSELCLV
                     EGSGTGWVVTLRGRRIPVAVRTWRERVLADAESASSRHDGPIEVRATLPGLVVAIAVK
                     EGDEVSSGATLLTIEAMKMQNEVRAPQAGRVAAIAVVAGQAVAAGAPLLRIE"
     gene            2056..3645
                     /locus_tag="VIN32_07960"
     CDS             2056..3645
                     /locus_tag="VIN32_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011984605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEY8922546.1"
                     /translation="MSDPERAARPPHRSTSGIQIRSVYRAADREAAHADEEPGAFPFT
                     RGIRPDGYLGRPWTMRQYAGFGSAAETNRRFRYLLASGQTGLSVAFDLPTQMGYDSDD
                     PIAVGEVGRVGVPIDTIADMEALFDGIGLDAVSTSMTINATAAILLTLYETVARRQGI
                     DPAILRGTIQNDILKEYIARGTYIYPPGPSMRLVTDTFAYCRAELPAWNTISISGYHM
                     REAGATAVQEVAFTIADAIAYSEAAVGAGMAFDEFAPRLSFFFAAHNDLFEEAAKFRA
                     ARAAWARVARDRFGASDPRSMAMRFHVQTGGSTLTAQQPETNVVRTTVQALSAILGGA
                     QSLHTNASDEALGLPTPATARLALRTQQVLAAESGVSSPVDALGGSYYVEALTDEIEA
                     RVHAELAEIDRRGGTLAALADGYQQAAIADAAYEAQRAIETGERIVVGVNAFAADGDE
                     QRPQAQVIDPQAEAEQVARTRAVRASRDAARAAETTAALEAAATGTENVLPRIRACVE
                     SQVTLGEISDALRRVWGEYRP"
     gene            3642..4076
                     /locus_tag="VIN32_07965"
     CDS             3642..4076
                     /locus_tag="VIN32_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008478685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY8922547.1"
                     /translation="MTIGPIDHIAVVVRSIDEALPRYRTLLGWEPIDAPRVFASQHVR
                     LCFLEPGEGYGAKVELVEPTDDESGVARFLATRGEGLHHVCLTSDDLASDLGALAAAE
                     AELIDREPRPGAHGEVAFIHPRTLNGVLWELLSTVGAEEPSA"
     gene            complement(4073..4951)
                     /gene="sucD"
                     /locus_tag="VIN32_07970"
     CDS             complement(4073..4951)
                     /gene="sucD"
                     /locus_tag="VIN32_07970"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEY8922548.1"
                     /translation="MSILVGAQTRLLVQGITGREGEFHSRAMLEYGTQIVAGVTPGKG
                     GQAAVDGRVPVFDTVADAVAQTAADAACIFVPAAFAPDAMLEAADAGIPLVICITEGI
                     PALDMLRVHHVLRSQGVRLIGPNCPGATTVGGAKVGIIPGDIHRPGPVGLVSRSGTLT
                     YEVVQAMTDAGVGQTTCVGIGGDPIVGTTFIDVLEMLNADPETEAIVLIGEIGGDEEE
                     RAADYLAREVRKPVAAFIAGRTAPAGRRMGHAGAIISGGAGTAESKRQALQAAGVRVA
                     DSPSEIPALLRDAGVR"
     gene            complement(4948..6087)
                     /gene="sucC"
                     /locus_tag="VIN32_07975"
     CDS             complement(4948..6087)
                     /gene="sucC"
                     /locus_tag="VIN32_07975"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008478179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HEY8922549.1"
                     /translation="MKLQEYRSKEILARYGVPIPGGETATSPDEARDAAARIGGPVVV
                     KAQVLVGGRGKAGGVKLAATPDEAAARTEEIIGLQIKGVTVRTVLVAQAARIAKEYYL
                     GLILDRGGQAVTIIASAEGGVEIEETAKANPEAILRLPLHPLIGLQEYQVRRVGFFLG
                     LPAELRKDFGATLRGLYAAFMESDADLAEINPLVVTDEGRLEALDAKIVLDDSALFRH
                     PDLESLRDLREEEPSEVAAREAGINFIKLDGSIGCMVNGAGLAMTTMDLVKLAGGEPA
                     NFLDIGGGAKADRVAAAFAIILDDPSVRAILINIFGGITRGDEVAHGIVEARASLGRQ
                     VPMVVRIVGTNATEAAQILRAANLITAESLDDAARKAVAAAGSHA"
     gene            6186..7196
                     /locus_tag="VIN32_07980"
     CDS             6186..7196
                     /locus_tag="VIN32_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HEY8922550.1"
                     /translation="MTYTVTLIPGDGIGPELTEATVSVLEATGIGFAWEVQQAGEATI
                     ASEGTPLPERVLESIRRNGVAIKGPITTPVGTGFRSVNVGLRLALELYANVRPARSMA
                     GVESRYTDVDLIIVRENTEDLYAGVEHRVGPDAAESIKIITRAASQRIARYAFEYALR
                     NGRRKVTAVHKANIMKLSDGLFLESCGQVAAEYAGRVEFEDRIVDNMCMQLVQKPELY
                     DVLVLPNLYGDIVSDLAAGLVGGLGVAPGSNIGETAAVFEAVHGSAPKYAGQNRANPT
                     ALILSSALMLRHLGETPAADRVESAVRAVIAEGRVTTHDLGGSAGTREFAAAVAERVR
                     GA"
     gene            7205..7573
                     /gene="sdhC"
                     /locus_tag="VIN32_07985"
     CDS             7205..7573
                     /gene="sdhC"
                     /locus_tag="VIN32_07985"
                     /inference="COORDINATES: protein motif:HMM:TIGR02970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HEY8922551.1"
                     /translation="MFRYRISWSQLAWLGHRLSGIGVLVYLFMHIVENATLAFGPGVY
                     DTTQTFFRNLPVRLGEIVLMAALVYHSLNGLRVVAMDFWPATTRWYRPVTYAVIIGTI
                     VAMIPLGLIMITPYAPWNGR"
     gene            7573..8034
                     /locus_tag="VIN32_07990"
     CDS             7573..8034
                     /locus_tag="VIN32_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase"
                     /protein_id="HEY8922552.1"
                     /translation="MAIGRRIWPVQGDPLRRNPSRAARPRPTEPGFERWMWYFTRISG
                     LLLVVLAMGHMLIMHVLVQLTGQEISFAFVTSRWGTPFWRIYDLLLLVLALVHGVNGA
                     RIVIGDYVERGGLRSLLIGMLLALTFVLLLLGIFVIATFDPNGAPAIGPFS"
     gene            8094..>8297
                     /locus_tag="VIN32_07995"
     CDS             8094..>8297
                     /locus_tag="VIN32_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006244272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEY8922553.1"
                     /translation="MVVGAGGAGLYAALELKRDLGPEARIGVISKLYPSRSHTGAAQG
                     GVCAALGNTEEDNWEWHWFDTVKG"
BASE COUNT         1186 a         2942 c         2899 g         1270 t
ORIGIN      
        1 atcccgctct gacctcccgt ctttgcgcaa agacgggccg ccgtttcctc gactcctcgt
       61 tgccaaccgc ggcgagattg cggtccgcat cattcgtgcc tgccgcgacc tggggatcga
      121 gtccatcgcc gcctacagcg acgccgacgc ggatgcgctg cacacgcgca tggcagatcg
      181 cactgagcgg atcggccccg catcggctgc cgagtcatac ctttcgatcg acgccgtgat
      241 ggcggccgct gtccgctccg gcgccgcggc aatacacccc gggtatgggt tcctgtcgga
      301 gaacgctgtc ttcgcccgcg ccgtcgagga tgcggggctc gtcttcgttg gaccgccgcc
      361 ggagacgctg gcatcgctcg gcgacaagct ggcagcccgc cggtccgttg ccgcggcggg
      421 ggtccccatc gtcccgggcc tgttggtccc gcttgtcgcc gacggcgacg gttccctcgc
      481 gaaggtggac ctcgcggggg tcagctttcc cgcattgctc aaagccgctg ccggcggcgg
      541 cgggcgcggg atgcgccagg tggctgatcg cagggagctc gaggcggcgc tgccggcagc
      601 cggtcgtgag gcgctcgcag cattcgggga tggaaccctc tacctcgagc agctcgtctc
      661 ccccgcacgg cacgtcgagg tccagctgct cggcgatcga catggcaacc tggcggtcct
      721 gggcgagcgg gaatgcagcg tccagcggcg tcatcagaaa ctggtcgagg agtcgccctc
      781 gccggcggtc ggccccgccc tgcgcgccga gctggccgag agcgcccgcc gcgtggccgg
      841 aacagtggcc ttccataacg ccgccaccgt cgagttcctg gtcgacaccg atggcgctca
      901 ctacttcctg gagatgaaca ctcgactgca ggtcgagcac ggggtgaccg agctggtgac
      961 cggcctcgat ctcgtcgcgt ggcagatccg ggtggcggcc ggcgagcaac tgtccgaatc
     1021 cgtcctggcg tcgacgccac ggggacatgc catcgaagtg cgcatctacg cggaggatcc
     1081 gcaccgtcgc ttcgccccgg tctcgggcac ggtcacgacc tggcggatgc cggctggacc
     1141 aggtgtcagg ctcgacgccg gcgtgaccgg tgacacgccc ctgccgctgg aatacgaccc
     1201 cctgctcgcc aagctcctgg tgcaggggga ggaccggcca gccgccgtgg cgcggttgcg
     1261 cagggccctc gacgagatcg cgatcggtgg cgtccagact gacctctcgt tcctgcgctg
     1321 gctggtcgac gaaccggcct tcgtgaccgg cgcctatgac accgggctca tccccaacga
     1381 ctggggtgat ggaccggcat tgaccgatct ggagcgctcc ctggcggctg aagttgcccg
     1441 agcggcgcgc gccagctcct ccccggggcc tgcagccgtc gcgccggtcg aggcggagag
     1501 cgcgtggcag caggtggcaa gacgcgaggc ggtcggacgg cggcgccccc ggtgagtgac
     1561 tacgagctga gcgtcgacgg caatcctgtc cagccggcca ccggctgggc tctcacctgg
     1621 ctcgacgccg agggcggggt cggccggctg accgacgggt cgcgctccga gctctgtctg
     1681 gtcgagggct cggggacggg ctgggtggtg accctgcgcg gccggcgcat cccggttgcg
     1741 gtgcgcacct ggcgggagcg tgtcctggcc gatgccgagt ccgcctccag ccggcacgac
     1801 ggcccgatcg aggtgcgagc cacccttccc gggctggtgg tggcgattgc cgtgaaggag
     1861 ggggacgagg tgtcatcggg cgcgaccctg ctcaccatcg aggcaatgaa gatgcagaac
     1921 gaggtccggg cgccgcaggc ggggcgggtt gcggcgattg ccgtggtcgc cggccaggcg
     1981 gttgcggccg gagcgcccct gctgcgcatc gagtagggcg ggtcggagtc gcccgcctcg
     2041 tacaatccga cggtgatgag cgaccccgag cgcgccgcgc ggcctccgca tcggtccacc
     2101 tcggggatcc agatccgatc ggtctaccga gccgccgacc gcgaggcggc ccacgcggac
     2161 gaagagcccg gggcctttcc cttcacccgc ggcattcgcc cggacgggta cctcgggcga
     2221 ccgtggacga tgcggcagta cgcgggcttc ggctccgccg ccgagacgaa tcgccgcttc
     2281 cgctacctgc tggcgagcgg ccagacgggc ctctcggtcg ccttcgacct gccgacccag
     2341 atggggtacg acagcgacga cccgatcgcg gttggtgagg tcggccgggt gggggtgccc
     2401 atcgacacca tcgccgacat ggaggctctc ttcgacggca tcgggctcga cgcggtgagc
     2461 accagcatga cgatcaacgc caccgccgcc atcctgctga ccctgtatga gacggtcgcg
     2521 cgccggcagg ggatcgaccc cgccatcctg cgtggcacca tccagaacga catcctcaag
     2581 gagtacatcg cgcgcgggac ctatatctac ccgcccgggc cctcgatgcg gctggtgacc
     2641 gacaccttcg cctattgccg agccgagctg cccgcgtgga acacgatcag catcagtggc
     2701 taccacatgc gcgaggcggg agccacggcc gtgcaggagg tcgccttcac catcgccgat
     2761 gccatcgcct actccgaggc cgcggtcggc gcggggatgg ccttcgacga gttcgccccg
     2821 cggctctcct tcttcttcgc ggcccacaac gacctgttcg aggaggcggc caagttccgc
     2881 gccgcccgcg ctgcctgggc gcgcgtcgcg cgcgaccggt tcggcgccag cgacccgcgc
     2941 agcatggcga tgcgcttcca cgtgcagacc ggcggcagca cgctcacggc ccagcagcca
     3001 gagacgaacg tggtgcgcac cacggtgcag gcgctgtccg ccatcctggg cggcgcgcag
     3061 agcctgcaca ccaacgcctc ggacgaagca cttgggctgc cgacgccggc cacggcccgg
     3121 ctcgccctgc ggacccagca ggtgctggcg gcggagtccg gcgttagcag tccggtggac
     3181 gccctgggcg ggtcctatta cgtggaggcc ctcaccgacg agatcgaggc gcgcgtacat
     3241 gccgagctgg cggagatcga tcgtcgcggc gggacgctgg cggccctggc agatggctac
     3301 cagcaggccg cgatcgccga cgcggcatac gaggcgcagc gcgcgatcga gacgggggag
     3361 cggatcgtcg tcggcgtcaa cgccttcgcc gcggatggcg acgagcagcg gccccaggcc
     3421 caggtgatcg acccgcaggc cgaggcggag caggtggcgc ggacgcgtgc cgtgcgggca
     3481 agccgcgatg cagcccgagc cgccgagacg accgctgccc tcgaggcggc cgccaccggc
     3541 acggagaacg tcctgccccg catccgggcc tgcgtcgagt cgcaggtcac gctgggtgag
     3601 atcagcgacg cgctccggcg cgtctgggga gaatatcggc cgtgaccatc ggcccgatcg
     3661 accacatcgc cgtcgtggtg cgctccattg acgaagcgct gccgcgctat cgcaccctcc
     3721 tcggctggga gccgatcgat gccccacgcg tcttcgcctc gcagcatgtc cgcctgtgct
     3781 tccttgagcc cggagaaggc tacggagcga aggtcgagct ggttgagccg accgatgatg
     3841 agagtggggt cgcgcgcttc ctggccacgc gcggcgaggg cctgcaccac gtctgcctga
     3901 ccagtgacga ccttgccagc gacctcgggg ctctggcggc ggcggaggcc gagctgatcg
     3961 accgcgagcc acgccccggc gcgcacgggg aggtggcctt catccatccg cgcaccctga
     4021 atggggtcct gtgggagctc ctgtcgacgg tcggcgcgga ggagccgagc gcctagcgga
     4081 ccccggcgtc gcgcagcagg gccgggatct cggacggtga gtcggcgacg cgaacgcccg
     4141 ccgcctgcag cgcctgccgt ttcgattccg cggtgccggc gccccccgag atgatcgccc
     4201 cggcgtggcc catgcgacgg ccagccggcg ccgtccgtcc ggcgatgaag gccgcgacgg
     4261 gcttccgcac ctcgcgagcc aggtaatccg ccgctcgctc ttcctcgtcg ccaccgatct
     4321 cgccgatcag cacgatcgcc tccgtctcgg ggtccgcatt gagcatctcc agcacgtcga
     4381 tgaaggtcgt tccaacgatc gggtcgccgc cgatcccgac gcacgtcgtc tggccaacgc
     4441 cggcatcggt catggcctgc accacctcgt aggtcagcgt accggagcgg ctgaccaggc
     4501 cgacgggccc ggggcggtgg atgtcgccgg ggatgatccc caccttcgca ccgccaacgg
     4561 tcgtggcgcc tgggcagttg gggccgatca ggcgcactcc ctgggagcgc agcacatggt
     4621 gcacgcgcag catgtcgagg gcggggatcc cctcggtgat gcagatcacc agcgggatcc
     4681 cggcgtctgc cgcctcgagc atggcatctg gcgcgaacgc agcggggacg aagatgcagg
     4741 ccgcatcggc ggcagtctgg gccaccgcgt cggcgacggt gtcgaagacc ggaactcgcc
     4801 cgtcgaccgc ggcctggccg cccttgccgg gggtcacccc tgccacgatc tgcgtcccgt
     4861 actccagcat cgccctcgag tggaactcgc cctcgcgtcc ggtgatgccc tgaaccagca
     4921 ggcgcgtctg cgccccgacc aggatgctca tgcgtgcgag cccgcggcgg cgaccgcctt
     4981 gcgggccgcg tcgtcaaggc tctcggcggt gatgaggttt gcggcgcgca ggatctgcgc
     5041 cgcctccgtc gcgttggtgc cgacgatgcg cacgaccatc ggcacctggc ggcccaggga
     5101 tgcccgggcc tcgacgattc cgtgcgccac ctcgtcgccc cgggtgatgc caccgaagat
     5161 gttgatgagg attgctcgca cgcttggatc gtcgaggatg atcgcgaagg cggccgccac
     5221 acggtccgcc ttggcgccgc cgccgatgtc caggaagttc gccggctcgc cccccgccag
     5281 cttgacgagg tccatcgtcg tcatcgccag tccggcgccg ttcaccatgc agccgatgct
     5341 gccgtcgagc ttgatgaagt tgatccccgc ctcgcgggcg gcgacctcag acggctcctc
     5401 ctcgcgcagg tcgcgcagcg actcgaggtc ggggtgacgg aagagggcgg agtcatcgag
     5461 gacgatcttg gcgtcgagcg cctccagtcg tccttcatcg gtcaccacca gcgggttgat
     5521 ctccgccagg tccgcatccg actccatgaa ggccgcatac agtccgcgca gggtcgcgcc
     5581 gaagtccttc cggagctctg ccggcaggcc caggaagaac cccactcggc ggacctggta
     5641 ctcctggagg ccgatcagcg ggtggagcgg caggcgcagg atcgcctcgg ggttggcctt
     5701 cgcggtctcc tcgatctcca cgcccccctc ggcgctggcg atgatggtga ccgcctgccc
     5761 gccgcggtcg aggatgaggc ccaggtagta ctccttcgcg atccgggcgg cctgcgcgac
     5821 cagcacggtc cggaccgtaa cgcccttgat ctggaggccg atgatctcct cggtgcgagc
     5881 ggcagcctca tccggggtgg ctgccagctt gacgccgccg gccttgccac gaccacccac
     5941 cagcacctgg gccttcacca ccaccggccc gccgatccgg gccgcggcgt cgcgcgcctc
     6001 gtcggggctt gtggctgtct cgccgccggg aatcggcacg ccgtagcggg ccaggatctc
     6061 cttggaacga tattcctgga gcttcacttc gccctcctgc gggcagaacg ggtggtcagc
     6121 cttcagccta cacttgccat ccctaccgcc ggcgctgtga ccggcgcgca ccggagacca
     6181 cgtccgtgac ctacaccgtc actctgatcc ccggcgatgg gatcggcccc gagctcaccg
     6241 aggccactgt cagtgtcctg gaggcgaccg ggatcggctt cgcctgggag gtgcagcagg
     6301 ccggcgaggc gaccatcgcg tcggagggga cgccgctccc ggagcgcgtc ctcgaaagca
     6361 ttcgtcgcaa cggggtggcg atcaagggac cgatcacgac cccggtcggc accggctttc
     6421 gcagcgtgaa cgtggggctg cggctggctc tggagctcta tgccaacgtg cgcccggccc
     6481 gcagcatggc cggcgtcgag tcgcgctaca ccgacgtcga cctgatcatc gtccgcgaga
     6541 acaccgagga cctgtacgcc ggcgtcgagc accgggtggg gcccgatgcg gcggagagca
     6601 tcaagatcat cacccgcgcc gcgtcgcagc ggattgcgcg ctacgcgttc gagtacgcgc
     6661 ttcgcaatgg gcggcgcaag gtcactgccg tccacaaggc caacatcatg aagctgtccg
     6721 acgggctctt cctggagagc tgcggacagg tggcggccga gtacgccggc cgcgtcgagt
     6781 tcgaggaccg gatcgtcgac aacatgtgca tgcagctggt ccagaagccg gagctgtacg
     6841 acgtgctcgt gctcccgaac ctgtatgggg acatcgtgag cgacctggcc gccggtctgg
     6901 tcggcgggct cggcgtggcc ccggggtcga acatcggcga gacggcagcc gtcttcgagg
     6961 cggtgcatgg gtcggcgccc aagtacgccg gccagaaccg cgccaacccg accgccctga
     7021 tcctctcgtc ggctctgatg ctccgccacc tgggcgagac gccggccgcg gaccgcgtcg
     7081 agtccgccgt gcgcgccgtc atcgcggagg gccgcgtcac cacccacgac ctggggggca
     7141 gcgcggggac ccgcgagttc gctgcggccg tggccgagcg cgtgcggggc gcctgactta
     7201 cttcatgttc cgctatcgga tcagctggtc gcagctggcc tggctggggc accgtctgtc
     7261 ggggatcggc gtcctcgtct atctcttcat gcacatcgtc gagaacgcga ccctggcctt
     7321 cggtccgggc gtgtacgaca cgacccagac cttcttccgc aacctgccgg tgcgcctcgg
     7381 cgagatcgtg ctgatggccg cgctggtcta ccactcgctc aacggcctgc gcgtcgtcgc
     7441 gatggacttc tggcccgcca ccacccggtg gtatcggccg gtgacctacg cggtgatcat
     7501 cggcaccatc gttgccatga tcccgctcgg gctgatcatg atcactccct acgcgccctg
     7561 gaacggccgc tgatggccat cggacgcagg atctggccgg tccagggcga cccgctccgc
     7621 cgcaacccgt cgcgcgctgc ccggccaagg ccgaccgagc ctggattcga gcgctggatg
     7681 tggtacttca cccgcatcag cgggctgctg ctggtggtgc tggcgatggg gcatatgctg
     7741 atcatgcacg tgctcgttca gctgaccggc caggagatca gcttcgcctt cgtcaccagc
     7801 cgatggggga ctccgttctg gcggatctac gacctgctcc tgctggtgct cgcgctggtg
     7861 catggcgtga acggggctcg catcgtcatc ggcgactacg tcgagcgagg cgggctgcgc
     7921 agcctgctga tcggcatgct gctggcgctg acgttcgtat tgctgctgct cgggatcttc
     7981 gtgatcgcca ccttcgatcc caacggcgcg cccgccatcg ggccattctc atgagcctcg
     8041 tgcgacaggg cgaaggcagc cctccacggc cgcggaccca cgacctcgat gcgatggtgg
     8101 tcggcgccgg gggggccggg ctgtacgccg ccctggagct gaagcgcgac ctcgggcccg
     8161 aggcccgcat cggcgtcatc agcaagctgt atccctcccg ctcccacacc ggggccgcgc
     8221 aggggggcgt gtgcgccgcg ctcggcaaca ccgaggagga caactgggag tggcactggt
     8281 tcgacacggt caaggga
//
LOCUS       DATCQR010000240        12695 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_44834, whole genome shotgun sequence.
ACCESSION   DATCQR010000240 DATCQR010000000
VERSION     DATCQR010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 12695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12695
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44834"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..715
                     /locus_tag="VIN32_08000"
     CDS             <1..715
                     /locus_tag="VIN32_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8922554.1"
                     /translation="LETPLRERRAALEELHLPERSAERFLYSQLATARSAEEVEAHFD
                     DARERRNEGLMVKDPASAYQPGRRGLGWLKLKKALATLDCVVVGVEWGHGKRRGVLSD
                     YTFAVRGPDGEALLTIGKAYTGLTDAEIATMTQHFKEITLRDFGRYRTVVPEVVVEIA
                     FDRLMRGMRHKSGFAMRFPRIVRIRDDKTPADIDDLATVEKLFAAQASGRILLARGAS
                     GVTERIATTGELADETAKG"
     gene            complement(712..1473)
                     /locus_tag="VIN32_08005"
     CDS             complement(712..1473)
                     /locus_tag="VIN32_08005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8922555.1"
                     /translation="MRLIHPVVVRGMRTNALAVALGMLVVGGVAVVLIALMPASRVAP
                     IALLGLSAALGLGVGGAMLWREARASHRRRNEIGDDIARLLAPAFDDTYTLVLAPRLP
                     GVPADLAALLVGPAGVRALIARRWHGRYRVRGRGWELDTRSRAGWIPTFTNPSFDADA
                     VADAVTRWSRTAINDPSILITPAIAFPRPYSTVVLEEPAGEIVTTDNAPWWAQGIGRV
                     QRLDAPRAARFVEAVLSASEAEAMAAAQRATPRVA"
     gene            1503..1994
                     /locus_tag="VIN32_08010"
     CDS             1503..1994
                     /locus_tag="VIN32_08010"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEY8922556.1"
                     /translation="MPTTLTALATVVDPGQGMRVDVVTGTGFRLIFDTADAAADRAGA
                     SPTESVLAALAACTSMDVGSILRKKRQPAASYRIAVTGEMSDEHPQVYTHIVVEHQVT
                     GDVDPQALRRAVELSATTYCPVSAMLSRSVPIEHRYRLSQGDRDEVSALVAITGPEGP
                     QVL"
     gene            2117..3055
                     /locus_tag="VIN32_08015"
     CDS             2117..3055
                     /locus_tag="VIN32_08015"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HEY8922557.1"
                     /translation="MARSMWRGAIQFGLVTIPVKLYLATETGGIGFNLLHKTDLSRIQ
                     MKVYCPADDEVISRSDTVRGYEWAKGRYVVVTDEDIESVPLKTVRAIEIEMFVDAHRE
                     GQGVEFVKQAYYLEPDPIGRKAFELLRQVLAEQGKTAICKIVLKDREQLAALNPYTKT
                     MLLTTLHWPDEIRSLEELDIPDEEIEVRPAERKMAEQLVASMTGEFKPEEYKDNYREA
                     LMAVIEAKVAGEAPAPAQKVEPTRIGDLMAALEASVASAREARREGATAKGSEAPAAK
                     AAKAAAKPARPRPKAARPRKAAAVDEAARPRQRKSA"
     gene            3064..3756
                     /locus_tag="VIN32_08020"
     CDS             3064..3756
                     /locus_tag="VIN32_08020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8922558.1"
                     /translation="MTAEQLPLPVPPSSDSPARRLPSRISLMQAMVVDAPFDDPGYLF
                     EPWWPGARAIAFCEHGSLRLQVAGMADALAAFPELIPMPEQLAEDGVVLDGTLLVLDD
                     AGRPDPELLRARLAGDGRPGHPAYVAGDLLWSGGQSVVARSFGVRRRWLEAILTPGDR
                     VTVGRAYVGDGTLVAEALAALGIDGLSARQLSARHRAGPAGDGWLRASLLAPEPRPRP
                     TLALILRLPLGD"
     gene            complement(3763..4407)
                     /locus_tag="VIN32_08025"
     CDS             complement(3763..4407)
                     /locus_tag="VIN32_08025"
                     /inference="COORDINATES: protein motif:HMM:NF014038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEY8922559.1"
                     /translation="MTSSQVEAELKFTARSERPLEELERATTLGPAHLGLARTIAEVD
                     RYLDTADLRLAAAHWACRLRSRDGRTIVSLKGPAQHAAGDMLHLRPEAEGPADSALDA
                     RAWQPSAARDQLLAMTGSAALVERFSLEQERTERSVSVDGTPVGELSLDRVGVLHDGI
                     QIGRMTVVELELDPGALARGFDHAPLSLALSAVDGLVADPSSKLERALAMLSGV"
     gene            complement(4404..5429)
                     /locus_tag="VIN32_08030"
     CDS             complement(4404..5429)
                     /locus_tag="VIN32_08030"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEY8922560.1"
                     /translation="MTDRGAWDDDARDDRLSRKRDRLARLLSVASILYARGSGETGVP
                     VAEIADLTGMTTRTVYRDIKALEEELAMPIFQAGKGRYGIEKKYFLPPLHLSLPEAVV
                     LFLASRLIARWSDAYDQAVVSAFTKLADLLPQPIARHVAATMLTVGQAEPNDQFVRNF
                     AHVAQAWADGRVVEFEYEPGEGERRSARVRPYFLEPDAAGRSVYLIGFDETVNAMRTY
                     KVERISSSTLTADSYQIPDDFDPDRWLAHSWGIWSSDTTATANVRLRFDASVAHRVRE
                     AVWHRSQRLTELPDGGVELAVTVAGIVEIRPWILSWGDGVEVLEPPELRDAVARALRG
                     AAARYQG"
     gene            complement(5426..6151)
                     /locus_tag="VIN32_08035"
     CDS             complement(5426..6151)
                     /locus_tag="VIN32_08035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8922561.1"
                     /translation="MPTTPSDAARLRRSTPKRHLVAMSALLLIVGGCATPSPTPTSSP
                     SPIPTAVPTPTPEPTPQPTPIFSNVPDPALVALIPTEAAGRAVQIPAITEFGITPGDI
                     GEVYGEIGTRFRSLQLAYISRPRLSLYAMRLNPPTVRTVDLEPYLAAAGEYVGIAGLH
                     REPWTLQIVAGHVVWGRPEDNATLPGTRVYSWAVDDLVFLMIGTDEAQNLSMLAGLPG
                     EAPPTPTPAPSTSGSPNAGPSAS"
     gene            complement(6192..9587)
                     /locus_tag="VIN32_08040"
     CDS             complement(6192..9587)
                     /locus_tag="VIN32_08040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEY8922562.1"
                     /translation="MAHTARDNAEGREALVSVAQAAALLGVHPNTVRSWTDAGRLPAY
                     RINARGDRRYRSGDVERLLAEGTASDAPPLVERRADAELGVLARLTGSGVSANATAVC
                     RIAVEALRSHLRIARVAAYLIREGGDGLLQLETHAGYQVSPPDTLDPAGGDQGEEGNP
                     VADIVVGPLRRQLALRAGGEIVGALVLEDDPDGPLAAVALTFLRTVATAVAANVLTAR
                     ALGRAKREVTRSRALRHVAQELAGQLDLTTVLDDIVDRTRTLFDADKAGLWLVEEGEF
                     PFHVAAGRGLGDDFLATTRRLTWDSAAVGVQAARERRSIVVRNADSRSAVGALQEDYQ
                     REGIKTACLVPLVSHDQSLGVIGLYHTRDREWPDDEVALAQSFANQAAVAISNARLYR
                     SVADQAARIRSIQDLSSRLNRLTDVQAIADAIVAEASTLAAYHDIRVYVVNWEEGFCE
                     PVAYTDRLLGEGDFHERLKVAIGDGSFTGWVAEHGEPLLINDALNDMRGHTIEGTDDI
                     EESMLVVPMVFEGHSVGVVALSKLGKDQFSNDDLQTMLIFAGYAAQAIANASAYERME
                     LQSTELARQLQSQRRLLEINERLLSSLDQQHVLDTIADGLRSVVHYDNLSIYRADHQN
                     RVMVPVLTRERHAEQVMRYLAPFGRGLMGWAVEHAEPVIANDALNDPRAMQIPGTPPD
                     PEALAIVPLVSDGEVIGCMNISRIGGEENYFSEQDFELVKLFAGQASIALRNADTHHA
                     VSERADTDALTGLGNHGSFQRTLGALAQDFAAEHGKGRGKGARAKQRPISLLMMDLDS
                     FKGYNDRLGHPAGDALLHAVGTAIYGAARSDDRVYRYGGDEFALVLPAVDAVAAAAIA
                     ERARQAVSRLTADDASPVTISIGVATLPGDATDKNELIAAADVALYHGKQSGGDRVTR
                     ASEVPGEMRDLRDTLDRSARTALLRPDQGTGAEAAHARRGSGTPIISTEEGVIDALLA
                     LAHSMDMRDPAGRGHADRVALLSGRLANQLGIHAQQRTDIELAARLHALDLVGTAEFE
                     SIRSLRPAAVLVRQHRAGMNPNEALIGSQIISVADAYDSFLSGVEGKRRGRATALAAL
                     RSAIGTRYRAEVVEALGALVAARTDGGQQRRQGDRQARERGAA"
     gene            complement(9742..10503)
                     /locus_tag="VIN32_08045"
     CDS             complement(9742..10503)
                     /locus_tag="VIN32_08045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8922563.1"
                     /translation="MPPSLMLPMWLGLGGLLLLALGLALIRRSGARLAIGRRLAGARQ
                     LRVGELLDLAPSDPLPRRPVRVLGRIRCAEPIVTSQDDRLVAFHRDVEVAMPRGGWRS
                     IERLRETRSFELWDHDGALQVDPAQAAEPLIVLPHVWSGSVEELDETYADGLARVAAE
                     QGSPGLARSTTRMVSVVDRLLLLAAVTRDAEGHVALAPPRGGYVVSALELDDAMRLLG
                     GPHPRLMLAGTASVALSVLLLAAAVLVAVAGLAAA"
     gene            10645..11649
                     /locus_tag="VIN32_08050"
     CDS             10645..11649
                     /locus_tag="VIN32_08050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8922564.1"
                     /translation="MSASHGAIARRNPAGNPRIAVYLAVPLILALAAIGVASAEVPPG
                     VTRVSAADAERAGSAIHLTQRPVGGSTLVEVKASTTGIRLLAVSADGTEVALADQLGE
                     ASGSLTLAEIDGEQLRIAMPGLLAASFAADGTWLAVIDGRGALWRVEAPSGRSALLAD
                     GPFIGSPVIEGDGSLLVLAVPSVEAPYQSRLVRLQPATGSVMRLSEEELVYGAFPLDD
                     GDLAIVAHETDGTVVHRLTGAGERLLVDLGLGAVNVAVAGNGRIAFERTGEGIYVVDL
                     PGPAARRIGAGSQPCFGPDGSSVLIRRAEQALALDLDGSVLAVSDEPAGFAASAECMP
                     "
     gene            11646..12641
                     /locus_tag="VIN32_08055"
     CDS             11646..12641
                     /locus_tag="VIN32_08055"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEY8922565.1"
                     /translation="MNGLVRRAAASVLLQLAILAIGGGSVLATDDYTLPFYDPNVTMS
                     YGVDRDRTLNRQLDWTGQVWNDGQPHWGRVYDQHTGVDYPMALRSEVAAAKDGTVVDF
                     EAGFGTTQFGSFGNFVKIKHADGRQTLYYHLASAADGGTSVWVGEAVYAGQRIGRSGC
                     SGLCYGAHLHFELLVWNSTAKAYKPADPLAERRWTTWPGRIPFDAGYVRESNGGTEVI
                     RRGWTIIHWVEFRNTGGRTWRSSGTVGRTALTTWNPASHASPFQAGDWPYSWMPTLVD
                     AASVPPDGVGRFTFGLKGSPAPGSYTETFNLQSYGIRWFDHQRLGGYYIPIVVTQ"
BASE COUNT         1909 a         4300 c         4539 g         1947 t
ORIGIN      
        1 gctcgaaacg ccgctgcgcg agcgtcgggc cgcgctggag gagctgcacc tgccggagcg
       61 tagcgctgag cgcttcctgt actcgcagct cgccacagcg cgctcggccg aagaggttga
      121 ggcccatttc gacgacgcgc gggagcgacg caatgagggc ctgatggtga aggaccccgc
      181 atcggcctat cagccggggc ggcgcgggct gggctggctg aagctcaaga aggcgcttgc
      241 caccttggat tgcgtggtgg tgggggtgga gtggggacac ggcaagcgtc gcggcgtcct
      301 gtccgactac acgttcgccg tgcgaggccc cgacggtgag gcgctgctca ccatcggcaa
      361 ggcctacacc ggcctgaccg atgcggagat cgcgaccatg acccagcact tcaaggagat
      421 cacgctgcgc gacttcggcc gctaccggac ggtcgtgccc gaggtggtgg tcgagatcgc
      481 gttcgatcgg ctcatgcgcg gcatgcgcca caagagtggt ttcgcgatgc gcttcccacg
      541 gatcgtccgg atccgcgacg acaagactcc cgcggatatc gatgacctgg cgacggtcga
      601 gaagctcttc gcggcccagg cgagcggtcg catcctgctg gcccgcggcg caagcggtgt
      661 gaccgaacgg atcgccacga ccggcgagct ggccgacgag acggcgaagg gctaggcgac
      721 ccggggcgtt gcccgctggg cggccgccat ggcttcggcc tcggaggcgc tgaggaccgc
      781 ctccacgaag cgcgccgcgc gcggggcatc cagacgctgg acgcgcccga tgccctgcgc
      841 ccaccacggg gcgttgtcgg tggtcacgat ctcgccggcc ggctcctcca gcaccaccgt
      901 ggaatagggt cgcgggaagg cgatcgctgg cgtgatgagg atgctggggt cgttgatcgc
      961 ggtccgtgac catcgcgtca ccgcgtccgc cacggcatcg gcgtcgaagg agggattcgt
     1021 gaaggtgggg atccagcccg cgcgtgagcg cgtgtcgagc tcccatcctc gtccgcggac
     1081 ccggtaccga ccgtgccagc gccgcgcaat cagggctcgc actccagccg gtccgacgag
     1141 cagagcggca aggtcagcgg gaacgcctgg caggcgcggg gccagcacca gcgtgtacgt
     1201 gtcgtcgaag gccggggcga gcagccgtgc aatgtcgtcg ccgatctcgt tgcggcggcg
     1261 gtggctcgcc ctcgcctccc gccagagcat ggctccccca accccgaggc caagggccgc
     1321 ggacaggccg agcagcgcga tcggggcgac tcggctggcc ggcatgagcg cgatgagcac
     1381 gaccgcgact ccccccacga ccagcatgcc aagcgcgacg gcaagggcgt tggtccgcat
     1441 gccacgaacg accaccgggt ggatcaggcg caccgggatt ctccggctgg taggctcacg
     1501 gcatgcccac cacgctcacc gcgttggcca ccgtggtgga tccggggcag gggatgcgcg
     1561 tcgacgtggt gaccgggacc ggcttccggc tgatcttcga caccgctgac gcagccgccg
     1621 atcgtgccgg tgcgagcccg accgaatcgg tgctggccgc cctcgcggcg tgcacctcga
     1681 tggacgtcgg cagcatcctg cgcaagaagc ggcagccggc ggcgagctac cggatcgcgg
     1741 ttaccggcga gatgtccgac gagcacccgc aggtctacac gcacatcgtg gtcgagcacc
     1801 aggtgacggg cgatgtcgac ccgcaggcgc tgcgaagagc ggtcgagctc tcggcgacga
     1861 cctattgccc ggtgagcgcg atgctgtcgc gctcggtgcc gatcgagcac cgctaccggc
     1921 tcagccaggg agaccgcgac gaggtcagtg cgctggtggc gatcacgggg ccggaggggc
     1981 cgcaggtcct ctaggggcat cattcgggga gtctcgcggt cgggcgatgg ccggccaatg
     2041 gcgtgatggt cctgccggag ccgcgtcctt tcgtcgaggt cggggctcgt tgcccgtccg
     2101 ttgtaggatc ttgcgcatgg cacgttcaat gtggcgcggc gcgatccagt ttgggctggt
     2161 gacgattccg gtcaagctct acctggcgac cgagacgggc ggcatcggct tcaacctgct
     2221 gcacaagacc gacctgagcc gcatccagat gaaggtctac tgccccgccg atgacgaggt
     2281 catctcgcgc tccgacacgg tgcgcgggta tgaatgggca aaggggcgct acgtggtcgt
     2341 gaccgatgag gacatcgagt cggtcccgct caagacggtc cgcgccatcg agatcgagat
     2401 gttcgtcgac gcacaccgcg aggggcaggg ggtcgagttc gtcaagcagg cctactacct
     2461 ggagccggac ccgatcggtc gcaaggcgtt cgagctgctg cgccaggtcc tcgccgagca
     2521 gggcaagacc gccatctgca agatcgtcct caaggatcgc gagcagctcg ccgccctgaa
     2581 cccgtatacc aagacgatgc tgctgacgac gctccactgg cccgacgaga tccgttccct
     2641 cgaggagctg gacatccccg acgaggagat cgaggtcagg ccagccgagc gcaagatggc
     2701 cgagcagctg gtcgcctcga tgaccgggga gttcaagccg gaggagtaca aggacaacta
     2761 ccgggaggcg ctgatggccg tgatcgaggc caaggttgcg ggagaggccc cggcgccggc
     2821 tcagaaagtc gagccgactc gcatcggcga cctgatggca gctctcgagg cgagcgtggc
     2881 cagcgcgcgc gaagctcggc gtgagggtgc cactgccaag ggcagcgagg ctcccgcggc
     2941 gaaggctgcg aaggctgccg ccaagcccgc caggcctcgc ccgaaggcag ccaggccccg
     3001 gaaggctgcg gccgtcgacg aggccgcgcg gccgcgacaa cgcaagagcg cctagccgtt
     3061 cgcgtgacgg cagagcagct tccgctcccg gtcccgccaa gcagcgactc gcccgctcga
     3121 cgtctgccgt cccgcatctc cctgatgcag gcgatggtgg tcgacgcccc gttcgacgac
     3181 cccggctatc tcttcgagcc gtggtggccg ggcgctcgtg ccatcgcgtt ctgcgagcac
     3241 ggcagccttc gtctccaggt cgcaggcatg gccgacgccc tcgccgcctt cccggagctg
     3301 atcccgatgc ccgagcagct ggccgaggat ggggtcgtgc tcgacgggac gctcctggtc
     3361 ctggatgacg ccggacgtcc ggatcctgag ctgctgcgag caaggctggc gggagacggc
     3421 aggcctggac accccgccta tgtcgccggc gacctgctgt ggagcggggg ccagtccgtg
     3481 gtggcgaggt cgttcggcgt tcgtcgacgc tggctcgagg cgatcctgac ccccggagac
     3541 cgcgtcaccg tggggcgtgc ctacgtcggc gatgggacgc tggtggccga ggcgctggca
     3601 gccctcggga tcgatggcct ctcggcgcga cagctgagcg cccggcatcg ggcaggcccg
     3661 gcgggcgatg ggtggctgcg cgcctccctg ctcgccccgg agccccgtcc gcgcccgacg
     3721 ctggcgctca tcctccgcct gccgctgggt gactgaggcc tctcagaccc cggacagcat
     3781 cgccagcgct cgttcgagct tgctggacgg gtccgcgacg aggccgtcaa ccgcagacag
     3841 cgccagcgac agcggcgcat ggtcgaagcc cctggccagc gcgccggggt ccagctcgag
     3901 ctcgaccacc gtcatgcggc cgatctggat cccgtcatgc aggaccccca cccggtcgag
     3961 cgacaactca ccgaccggtg ttccgtccac gctcacggat cgttccgtgc gctcctgctc
     4021 gaggctgaac cgctccacga gggcagcgct gccggtcatg gcgagcaact ggtcacgcgc
     4081 cgctgatggc tgccaggcgc gcgcatcgag agcggaatcc gccggccctt cggcctcggg
     4141 tcggaggtga agcatgtcgc cggccgcatg ctgagcgggc cccttcagcg agacgatcgt
     4201 gcgcccgtca cgcgaccgca gccggcatgc ccaatgggcg gcagccaggc gcaggtcggc
     4261 cgtgtccagg tatcggtcca cttcggcgat ggtccgcgcg agaccgaggt gtgctgggcc
     4321 aagcgttgtc gctcgctcca gctcctccag tgggcgctcg gagcgggcgg tgaacttgag
     4381 ctcggcttcg acctgcgacg aggtcatccc tggtagcgcg cggcggcgcc gcggagcgcc
     4441 cgcgcgacag catcgcgcag ctcaggcggc tcgagcacct cgacgccatc gccccacgac
     4501 aatatccagg gtcgaatctc gacgatccca gccaccgtca ccgccagctc gacgccgcca
     4561 tcgggcagct cggtgaggcg ctgggagcgg tgccagaccg cctcccgcac gcggtgcgcg
     4621 acggaggcat cgaaccgcag gcgcacattc gccgtggcgg tcgtgtccga cgaccagatc
     4681 ccccacgagt gggcgagcca acggtcgggg tcgaagtcgt cggggatctg gtagctatcc
     4741 gccgtcaggg tcgagctgct gatccgctcc accttgtagg tccgcatcgc gttcaccgtc
     4801 tcatcgaagc cgatcaggta gacgctgcgc ccggcggcat cgggctccag gaaatacggg
     4861 cgcacgcgcg cgctgcgacg ctcgccctcg ccgggctcgt actcgaactc gaccacccgc
     4921 ccatcagccc acgcctgggc aacgtgggcg aagttgcgca cgaactggtc gttcggctcc
     4981 gcctggccga cagtcagcat cgtggccgcg acgtgccgcg cgatcggctg tggaagaagg
     5041 tccgccagct tggtgaaggc gctgaccacc gcctggtcgt acgcatcgga ccagcgggcg
     5101 atcaggcgcg acgcaaggaa gaggacgacc gcctccggca gggagaggtg caggggcggg
     5161 aggaagtact tcttctcgat cccgtagcgt cccttgccgg cctggaagat cggcatcgcc
     5221 agctcctcct ccagggcctt gatgtcgcga tacaccgtgc gtgtggtcat gccggtcagg
     5281 tcggcgatct cggcgaccgg caccccggtc tcaccgctgc cgcgggcgta gaggatcgag
     5341 gcgaccgaga ggagccgggc gagccggtcg cgcttgcgcg agaggcggtc gtcgcgagcg
     5401 tcgtcgtccc acgctccgcg atcggtcatg aggcggacgg ccccgcgttc gggctcccgc
     5461 tggtcgaggg cgccggcgtt ggcgtgggcg gcgcctcccc gggcaggccg gcaagcatgg
     5521 agagattctg agcctcgtcg gtcccgatca tcaggaagac gaggtcgtcc accgcccagc
     5581 tgtagacgcg cgtgccgggg agcgtggcat tgtcctcggg gcgtccccag accacgtgcc
     5641 cggccacgat ctggagggtc catggctccc gatgcagccc ggcgatcccg acgtactcgc
     5701 cggcggcggc caggtagggc tcgaggtcca cggttcgcac cgtcggcggg ttcaggcgca
     5761 tggcatacag cgagagacgc ggtcgcgaga tgtacgcgag ctggagggag cggaaccggg
     5821 tgccgatctc gccgtacacc tcgccgatgt caccgggcgt gatcccgaac tcggtgatgg
     5881 ccgggatctg gaccgcgcgt ccggccgcct cggtcgggat cagcgcgacc agcgcaggat
     5941 cgggaacgtt gctgaagatc ggggtcggct gcggggttgg ttccggggtg ggagttggga
     6001 ccgctgtcgg gatcgggctt gggctggagg tcggtgtagg tgacggcgtt gcgcagccgc
     6061 cgacgatcag gagtagggcc gacatcgcga cgagatgccg cttcggggtg gaccgccgca
     6121 ggcgggcagc gtccgatggg gtggtcggca tgggaggcgc aatgatagcg cccggcgcgg
     6181 acctgggcgg gtcaggcagc tccccgttcg cgagcctgcc ggtcgccctg ccgccgttgc
     6241 tgaccaccgt ccgtgcgagc cgctaccagc gccccgagcg cctcaaccac ctcggcccga
     6301 tagcgggtcc cgatcgcgga gcgcagcgcg gccagggctg tggcgcggcc tcgccgcttc
     6361 ccctccacgc cgctcaggaa cgagtcgtag gcatcggcga ccgagatgat ctgggaaccg
     6421 atcagggctt cgttcgggtt catcccggcg cggtgctggc ggacgagtac cgccgcgggg
     6481 cgcagcgagc ggatcgactc gaactcggcg gtgccgacca ggtcgagcgc gtgcagtcga
     6541 gctgcgagct cgatgtcggt gcgctgctgg gcatggatgc cgagctggtt tgcgagcctg
     6601 cccgacagga gggccacccg gtcggcatgg ccccgtcccg ccggatcccg catgtccatg
     6661 gaatgggcaa gggccaggag ggcgtcgatc acgccttcct cggtcgagat gatcggcgtc
     6721 cccgagccgc gacgcgcgtg cgcggcctct gccccggtcc cctggtcggg acggaggagc
     6781 gcggtgcgcg ccgaccggtc gagggtgtcg cggaggtcgc gcatctcgcc tggaacctcc
     6841 gaggcgcgcg tgacccggtc gccgcccgac tgcttgccat ggtagagggc gacgtcggct
     6901 gcggcgatca gctcattctt gtcagtggcg tcgccgggca gcgtcgcgac gccgatcgag
     6961 atggtgaccg gcgaagcgtc gtcagcggtg agtcgcgaga ccgcctgacg cgcccgctcg
     7021 gcgattgctg cggccgcgac ggcatcgacc gccggaagaa ccagggcgaa ctcatcgccg
     7081 ccgtatcggt acacccggtc gtcgctccgc gctgcgccgt agatggcggt ccccaccgcg
     7141 tgcagcagcg cgtcgccggc cgggtggccg agccggtcgt tgtacccctt gaaggagtcg
     7201 aggtccatca tgaggagcga gatggggcgc tgcttggcgc gcgctccctt tccgcggccc
     7261 tttccgtgct cggcggcgaa gtcctgggcc agggcgccca atgtccgctg gaaggatccg
     7321 tggttcccca gcccggtcag tgcatcggtg tcggctcgct cgctgacggc gtggtgcgta
     7381 tcggcattgc gcagggcgat cgacgcctgg ccggcgaaga gcttgaccag ctcgaagtcc
     7441 tgctcgctga agtaattctc ctcgccgccg atgcgggaga tgttcatgca cccgatcacc
     7501 tcgccgtcgc tcaccagcgg cacgatcgcc agcgcctccg ggtcgggggg cgtgccaggg
     7561 atctgcatcg cccgtgggtc gttcagggcg tcattggcaa tcaccggctc ggcgtgctcg
     7621 accgcccagc ccatcaggcc gcggccgaac ggtgcgaggt agcgcatcac ctgctctgca
     7681 tgccgctccc gcgtcaggac gggcaccatg acgcggttct ggtggtcggc gcggtagatc
     7741 gacagattgt cgtagtggac caccgagcgg aggccgtcgg cgatcgtgtc aagcacgtgc
     7801 tgctggtcga ggctcgacag caggcgctcg ttgatctcaa gcaggcggcg ctgtgactga
     7861 agctggcggg cgagctcggt cgactgcagc tccatgcgct cataggccga tgcgttggcg
     7921 attgcctgcg ccgcgtagcc ggcgaagatc agcatcgtct gcaggtcgtc gttgctgaac
     7981 tggtccttgc cgagcttcga gagggcgacc acgccgacgg agtgaccctc gaacaccatc
     8041 ggcacaacca gcatcgactc ctcgatgtcg tcggtcccct cgatggtgtg gccgcgcatg
     8101 tcattgagcg cgtcgttgat cagcagcggc tcgccgtgct cggctaccca gccggtgaac
     8161 gaaccgtcgc cgatggcgac cttcaggcgc tcatggaagt cgccctcacc cagcagccga
     8221 tccgtgtagg cgaccggctc gcagaagccc tcctcccagt tcacgacgta gacccggatg
     8281 tcgtggtagg cggcaagggt gctcgcctcc gcgacgatcg catcggcgat cgcctgtaca
     8341 tcggtcaggc gattgagcct ggatgagagg tcctggatcg atcgaatgcg ggcggcctgg
     8401 tcggccaccg agcggtacag gcgcgcgttg ctgatggcca ccgcggcctg gttggcgaat
     8461 gactgcgcca gggcgacctc gtcatctggc cattcccggt cgcgggtgtg gtagaggccg
     8521 atgacgccca gtgactggtc atggctgaca aggggcacga gacaggctgt cttgattccc
     8581 tcccgctgat agtcctcctg cagagcgccg acggcggacc gactgtcggc gttgcggacg
     8641 acgatgctgc ggcgctcccg agccgcctgg acgcctaccg cggcgctgtc ccaggtgagc
     8701 cggcgagtgg tcgccaggaa gtcgtcgcca aggccccgcc cggctgcgac gtggaacggg
     8761 aactcaccct cctcgaccag ccacagccca gccttgtcgg cgtcgaagag ggttcgggtg
     8821 cggtcgacga tgtcatccag aacggtggtc agatccagct gaccggcgag ctcctgggcg
     8881 acgtgacgga gggcccgcga tcgcgtgacc tcgcgcttgg cgcgaccaag agcgcgcgcc
     8941 gtgaggacat tggcagccac tgcggtcgcg acggttcgca ggaaggtaag cgccacggcg
     9001 gccaggggac catcggggtc gtcctcgagg acgagcgcac ccacgatctc accgccggcc
     9061 cgcagcgcga gctggcggcg gaggggtccg accacgatgt cggcaaccgg gttgccttcc
     9121 tctccttggt cgccgcccgc gggatcgagg gtgtcgggag gcgagacctg gtatccggcg
     9181 tgggtctcga gctgaaggag gccgtcgcct ccttcccgga tgagatatgc ggcaacccgc
     9241 gcgatgcgaa ggtgtgaacg cagggcctcg accgcgatcc gacagaccgc ggtggcgttg
     9301 gcggagactc cggatcccgt cagtcgggcg agcaccccca gctcggcgtc ggcacgccgt
     9361 tcaacgagtg gcggcgcatc cgacgcagtg ccctcggcca ggagccgctc gacgtcgccg
     9421 ctccggtagc gccggtcgcc gcgtgcgttg atccggtatg cggggagcct ccccgcgtcg
     9481 gtccaggagc ggaccgtatt ggggtgcacg cccagcaggg cagcggcctg ggcgacgctg
     9541 accagcgcct cccggccctc cgcattgtcg cgagctgtgt gcgccaactc tccgctcctg
     9601 ctctggagtc attggaactt ctggtaagtc ccgtgcagcg gatggtagaa ctgggtagca
     9661 gcggcctcga tgccccgttc gtaccccgtc gcccccgtac tcgccggccg gcaatgcctg
     9721 gccaggaacg cgtctgcccg gtcaggcggc ggcgagcccg gcgaccgcca cgagtactgc
     9781 ggctgccagc agcagcaccg agagggccac cgatgctgtt ccagccagca tcagccgcgg
     9841 gtgggggccg ccaagaagcc gcatcgcgtc gtccagctcc agcgcgctga cgacgtatcc
     9901 gcctcgcggt ggtgccaggg caacgtgccc ttccgcgtcc cgcgtcaccg ccgccaacag
     9961 gagcaatcga tccacgacgc tgaccatccg ggtcgtggat cgggcaaggc cgggagaacc
    10021 ctgctcggca gccacccgcg ccaggccatc ggcgtaggtc tcgtccagct cctcgaccga
    10081 ccctgaccac acgtgtggca gcacgatcag cggctccgct gcctgggcag gatcgacctg
    10141 gagagctccg tcgtggtccc acagctcgaa tgagcgcgtc tcgcgcagcc gttcgatgct
    10201 cctccagccg ccgcgtggca ttgcgacttc gacgtcgcga tggaaggcga cgagccggtc
    10261 gtcctgcgag gtgacaatcg gctccgcaca ccgaatgcgg ccaaggacgc gcaccggtcg
    10321 acgcggcagg ggatcgctcg gcgcaaggtc gagcagctct ccgacgcgca gctgtcgagc
    10381 gcctgccagc cgccggccga tggcgagtcg ggcccctgag cgacggatga gcgcgaggcc
    10441 gagtgccagc agcaggagcc cgccaaggcc gagccacatc ggaagcatca gggatggggg
    10501 catatgacca cgttcacctc gcgataagtc tgcggtaagc ggccgtgact aggctcgcag
    10561 gcgtcgtcat ctcatcactg gaggtctgtc gccaccggag cgtttgtcgt cattggtcgt
    10621 ttttcaacgc tacgggaggt ctccatgtcc gcgtcccatg gcgccatcgc gcgccgcaac
    10681 ccggctggca atccgcgcat tgccgtctac ctcgccgttc ccctcatcct cgccctcgcg
    10741 gccatcgggg tcgcctcggc cgaggtgccg cccggcgtga cgcgcgtgag cgcggcggac
    10801 gccgagcggg cgggatcggc gatccacctg acccagcggc ccgtcggtgg ctcaacgctg
    10861 gtagaggtca aggcatccac caccggcatc cgcctgctgg ccgtctcggc agatggcacc
    10921 gaggtcgcgc ttgccgacca gctgggcgag gcgtccggat cgctcacgct ggcggagatc
    10981 gacggcgagc agctccgcat cgcgatgccc ggcctgctgg cggcatcgtt cgccgccgat
    11041 ggcacctggc tggcggtgat cgacggtcgt ggcgcactgt ggcgggtcga ggctccttcc
    11101 ggccggagcg cgctcctggc cgatggcccc ttcattggct cccccgtcat cgagggcgat
    11161 ggatcgctcc tggtgctcgc cgttccgtct gtcgaggcgc cgtaccagtc gcgcctcgtg
    11221 cgcctgcagc cggccaccgg cagcgtcatg cgactgtcgg aggaggagct ggtctatggg
    11281 gccttcccac tcgacgacgg cgacctggcc atcgtggcgc acgaaacgga tgggaccgtg
    11341 gtccatcggc tcacgggcgc gggcgagcgg cttctggtcg atctcggcct cggggctgtc
    11401 aatgtcgcgg ttgccgggaa tggccgtatc gcgttcgagc gaaccggcga ggggatctat
    11461 gtcgtcgacc tgcccggtcc cgctgcccgg cgtatcggcg ccgggtcgca gccgtgcttt
    11521 ggaccggatg gatcctcggt gctcatccgg cgcgcggagc aagcgctggc gctggacctt
    11581 gacgggtcgg tgctggccgt ctcggacgag ccggccggct ttgccgcttc ggcggagtgc
    11641 atgccatgaa cggcctggta cgccgcgcgg cggcatcggt cctccttcaa ctcgccatcc
    11701 tggcaatcgg cggcggatcg gtcctcgcca ccgacgacta caccctcccg ttctacgacc
    11761 cgaacgtgac gatgagctac ggggtcgacc gcgatcggac cctgaaccgt cagctcgact
    11821 ggaccgggca ggtctggaat gacgggcagc cgcactgggg gcgggtgtac gaccagcaca
    11881 ccggggtcga ctatccgatg gcattgcgca gcgaggtcgc ggcagccaag gacgggacgg
    11941 tcgtggactt cgaggccggc ttcggaacga cgcaattcgg ctcattcggg aatttcgtga
    12001 agatcaagca cgccgatggc cggcagaccc tctactacca cttggccagc gccgccgatg
    12061 gcgggacctc ggtctgggtc ggggaggccg tctacgccgg ccagcgcatc gggcgatccg
    12121 gctgcagtgg cctctgctac ggagcgcatc tgcacttcga gctgctcgtc tggaattcca
    12181 cggccaaggc gtacaagccg gccgacccgc tcgccgagcg ccgatggacg acctggccag
    12241 ggcgtatccc gttcgatgcg ggctacgttc gcgagagcaa cggcggcacc gaggtgatcc
    12301 gccggggatg gacgatcatc cattgggtcg agttccgcaa caccggcggc cggacctggc
    12361 gatccagcgg gacggttggt cggactgcgc tgaccacctg gaaccccgcg tcccacgcct
    12421 ccccgttcca ggccggcgac tggccgtaca gctggatgcc gacgctggtg gatgccgcca
    12481 gcgtgcctcc cgatggggtc ggtcgcttca ccttcggcct gaagggatcg cccgcgcccg
    12541 gcagctacac cgagaccttc aacctccagt cgtacggcat tcgctggttc gatcaccaga
    12601 ggttgggtgg ctactacatc ccgatagtgg tgacgcagtg aaggctctgg tggcggtgct
    12661 cgcgatcttc gtcggcgggt gttcggtggc ggggg
//
LOCUS       DATCQR010000241         2851 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_198783, whole genome shotgun sequence.
ACCESSION   DATCQR010000241 DATCQR010000000
VERSION     DATCQR010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2851
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198783"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /locus_tag="VIN32_08060"
     CDS             <1..257
                     /locus_tag="VIN32_08060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8922566.1"
                     /translation="VRAEVVHAQLGDHGGGAGTRPRRTHPAAIRPAVAGRRDEIDPLD
                     EWVRGLDGRAAYVDRLGAARVDGLRPEPRPSGSVDYGSYR"
     gene            254..994
                     /locus_tag="VIN32_08065"
     CDS             254..994
                     /locus_tag="VIN32_08065"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013133068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HEY8922567.1"
                     /translation="MSAATPDELMIAAAAAELAGVHTVFVGIGLPNAAANLARLSVAP
                     DLELIYESGVVGARPTRLPDSIGDPALVSGAAATMSMRDLFGGFLQGGRIEVGMLGAA
                     QIDRWGNLNTTVIGSYDRPSVRLPGSGGACEIALNAQRVVVVMIQSRRTFVERVDFVT
                     SPGHRRPGGSGVRPSWAGAGPTAVVTQLGVYHFGPDGEMELAGLHGGVDDQTVDAGTG
                     WAMRHADTVTRLPEPSAQTLEAIRRLVA"
     gene            complement(991..2112)
                     /locus_tag="VIN32_08070"
     CDS             complement(991..2112)
                     /locus_tag="VIN32_08070"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEY8922568.1"
                     /translation="MTPRRLAAGLAVVLLVAACAPAVSPSPSASVRSSKQPAPIETPF
                     GTVLPSEVESSAPSATPASAGTAYTVKPGDTLSLIARTWGTTVQQLQAWNAASHPSLV
                     TDPNTLQTGWVLIVAGDPGTTPLPTVAPTTRPTPAPSGCRAGNRLAAGGQQTFTTVPN
                     AGHGVALTLDMGGRLDPGLDILNLLISNHVCATIFPTGAMAQTTQGRQVMALIKAHPE
                     LFEIGNHTMHHCDLVRGGAGSPTTAPCAGVTPTADFIRRELTDAAAILAQLSGQQPVP
                     YWRPPYGSVNQAVRDAAASVGYTKTFLWDIDTIDWKPIADGGPTAQQIATKVIGNARD
                     GSNVLMHLGGYETLAALRIMIPGLRDRGFLLTSLSDLLN"
     gene            complement(2118..>2851)
                     /locus_tag="VIN32_08075"
     CDS             complement(2118..>2851)
                     /locus_tag="VIN32_08075"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HEY8922569.1"
                     /translation="TCACHHRPAMRLPFFPLHVVLFPHLPLPLHIFEERYRAMTRDLL
                     ADGSPYAGRFVIGSGGDEDVPQGSVGTVAEVRRAEQFADGRWALVVVGAERVALGAVD
                     RSGPYALIEVQPLPESVGDTDTAGRLLPEVQAALDAYLDTVKRFVTSAASVGRESQEI
                     TDVAASLDEVLKPIRLPDDPLAASYAVAGVLQIELNRKQQLLELPDGASRLEAELNLL
                     RREARLLADGAMPTIRREDLRYHPN"
BASE COUNT          411 a          956 c         1064 g          420 t
ORIGIN      
        1 ccgtccgggc cgaagtggta cacgcccagc tgggtgacca cggcggtggg gccggcaccc
       61 gcccacgacg gacgcacccc gctgccatca ggccggcggt ggccgggcga cgtgacgaaa
      121 tcgacccgct cgacgaatgg gtccgcggcc tggatgggcg ggccgcctat gtcgaccggc
      181 tgggggccgc gagggtcgat gggctgcgcc ccgagccgcg cccctcggga agcgtcgact
      241 atgggagcta ccggtgagcg ccgccacgcc ggacgagctg atgatcgccg ccgcggccgc
      301 ggagctggca ggggtgcaca cggtcttcgt cgggatcggc ctgccgaatg cggccgccaa
      361 cctggcacgc ctgtcggtgg cacccgacct ggagctgatc tacgagtccg gggttgtcgg
      421 agcacggccg acccgcctgc ccgacagcat cggcgacccg gcgctcgtgt cgggggcggc
      481 tgccaccatg agcatgcgcg acctgttcgg cggcttcctg cagggcgggc ggatcgaggt
      541 gggaatgctg ggcgcggccc agatcgaccg ctggggcaac ctgaacacga ccgtcatcgg
      601 ctcctatgac cgccccagcg tgcggctgcc gggttcgggt ggagcctgcg agatcgccct
      661 caatgcgcaa cgagtggtgg tcgtcatgat ccagtcgagg cgcacattcg tcgagcgggt
      721 cgatttcgtc acgtcgcccg gccaccgccg gcctggtggc agcggggtgc gtccgtcgtg
      781 ggcgggtgcc ggccccaccg ccgtggtcac ccagctgggc gtgtaccact tcggcccgga
      841 cggcgagatg gagctggccg ggctgcatgg cggcgtcgac gatcagacgg tcgacgcagg
      901 cacgggctgg gcgatgcgac acgcggacac cgtcacgagg ctgccggagc catcggctca
      961 gaccctggag gcgattcgcc ggctggtcgc ctagttcagc aggtcggaga ggcttgtcag
     1021 caggaagccg cggtcgcgca ggccggggat catgatccgc agggcagcga gcgtctcgta
     1081 gccgcccagg tgcatcagga cgttcgaccc gtcgcgggcg ttgccgatca ccttggtggc
     1141 gatctgctgg gctgtcgggc ccccgtcggc gatcggcttc cagtcgatcg tgtcgatgtc
     1201 ccacaggaag gtcttcgtgt acccgaccga tgccgcggcg tcgcgcaccg cctggttcac
     1261 cgatccgtac ggcgggcgcc agtacgggac cggctgctgg cccgagagct gcgccaggat
     1321 ggccgccgca tcggtcagct ccctgcggat gaagtcagcg gtcggagtca ccccggcaca
     1381 gggcgcggtg gtcggcgagc ctgccccacc tcgcaccagg tcacagtggt gcatggtgtg
     1441 gttgccgatc tcgaacagct ccgggtgggc cttgatgagc gccatcacct gcctgccctg
     1501 ggtggtctgc gccatggccc cggtcgggaa gatggtcgcg cagacgtggt tcgagatcag
     1561 caggttgagg atgtcgagcc ccgggtcgag gcggccaccc atgtccaggg tgagggccac
     1621 cccgtgcccg gcgttcggga ccgtggtgaa ggtctgctgc ccgcccgccg ccaggcggtt
     1681 gccggcccgg catccgctgg gtgcaggcgt gggcctggtg gtaggagcga cggtgggaag
     1741 cggcgtcgtc cccgggtcgc cggcgacgat caggacccag ccggtctgga gcgtgttcgg
     1801 gtcggtgacg agcgatggat gggacgcggc attccaggcc tgaagctgct gcacggtcgt
     1861 cccccaggtg cgcgcgatca gggagagcgt atcgcccggt tttacggtgt aggccgtgcc
     1921 cgccgaggcg ggcgtcgcgc tcggtgcgga cgattcgacc tcgctcggca ggaccgtccc
     1981 gaatggcgtc tcgatcggcg cgggctgttt gcttgagcga acgctcgcgc tgggcgatgg
     2041 actgacggcg ggggcgcagg cagccaccag cagcacgacc gccaggccag cggcgaggcg
     2101 gcgaggagtc atggcgatca attggggtgg tagcgaaggt cttcgcggcg gatcgtcggc
     2161 atggccccgt cggccaggag gcgcgcctca cggcgcagca ggttcagctc ggcttccagg
     2221 cggcttgctc catcaggcag ctccaggagc tgctgcttgc ggttcagctc gatctgcagc
     2281 acccccgcca ccgcatagct ggcagccagc ggatcgtcgg ggagacggat cggcttgagc
     2341 acctcgtcga gggacgctgc cacgtcggtg atctcctgcg attctcgccc cacagacgca
     2401 gccgacgtca cgaagcgttt caccgtgtcg agatacgcat ccagcgcggc ctgcacctcc
     2461 gggagcaggc gcccagcggt gtcggtgtct cctaccgact ccggcagggg ctgcacctcg
     2521 atcagcgcat atggcccgga ccgatccacc gcacccagcg ccacccgctc cgcgccgacc
     2581 acgaccagcg cccagcggcc gtcggcgaac tgctccgcgc ggcgcacctc tgcgacggtt
     2641 ccgacagagc cctgcggcac gtcctcgtcg ccgccgctgc cgatcacgaa ccggcccgca
     2701 tacggagagc catcggccag gaggtcgcgg gtcatggccc gatagcgctc ctcgaagatg
     2761 tggagcggca gcggcaggtg cgggaagagg acgacgtgga gcgggaagaa cggaagccgc
     2821 atggccggcc gatggtggca cgcgcacgtt g
//
LOCUS       DATCQR010000242         1540 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_184248, whole genome shotgun sequence.
ACCESSION   DATCQR010000242 DATCQR010000000
VERSION     DATCQR010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184248"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /locus_tag="VIN32_08080"
     CDS             complement(<1..391)
                     /locus_tag="VIN32_08080"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY8922570.1"
                     /translation="MSEERRIVTVLFADVTGSTAMGEELDPEEVRALLGRYYEIAKVI
                     IAEHGGTLEKFIGDAVMAVFGLPAAHGDDAERAVAAALALRAGVRADGRLRDRLAIRF
                     GVSTGDVVATRDPTAHDFLVTGDAVNVA"
     gene            complement(396..1145)
                     /locus_tag="VIN32_08085"
     CDS             complement(396..1145)
                     /locus_tag="VIN32_08085"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HEY8922571.1"
                     /translation="MQGPYTMRQRLEAARAEGRIMLGGHRGNPAEHPENTLASFRSAI
                     DAGCDLVECDVHLTADGKLAVIHDHLLERTTNGTGLVGAHTLAELRELDAGKGQKIPL
                     LEELFELVRDRVGLVIETKQNPVPYPGLEEKLAAVLRENDMVERVSVISFHHRCIRHL
                     KEVAPELDLGIIDASRPIDPVAMLRASGADLFSSYYATLDPEMAEEIRAAGGAVGVWT
                     VDDQLSTMWARACHPDSVFTNRPAEIGPLLN"
     gene            1173..>1540
                     /locus_tag="VIN32_08090"
     CDS             1173..>1540
                     /locus_tag="VIN32_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007923295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase"
                     /protein_id="HEY8922572.1"
                     /translation="MSSTEPRTIAELRASGHQRETVKQEMRRNLLARVRERRPIFPGI
                     VGYEETVIPQVSNAVISGHDLIFLGERGQAKSRLMRSMTELLDSEVPVMAGCEINDHP
                     FAPICKRCRDEVEASGEEAA"
BASE COUNT          248 a          551 c          506 g          235 t
ORIGIN      
        1 ccgccacgtt gaccgcgtcg ccggtgacca ggaaatcgtg ggccgtcgga tcccgcgtcg
       61 ccaccacgtc gcccgtggac acaccgaagc ggatcgccag ccgatctcgc agccgcccgt
      121 ccgcgcgaac cccggcgcgc agcgccagcg ccgcggcaac cgcccgctcg gcgtcgtcgc
      181 cgtgcgcggc cggcaggccg aagaccgcca tcaccgcatc accgatgaac ttctcgagcg
      241 tcccgccatg ctcggcgatg atgactttgg cgatctcgta atagcgcccg aggagggcgc
      301 ggacctcctc gggatcgagc tcctcgccca tggcggtaga gccggtcaca tcggcgaaga
      361 gcacggtcac gatccgccgt tcctcgctca cgccttcagt tcaggagggg cccgatctcg
      421 gccggccggt tggtgaagac ggaatcggga tggcaggcgc gcgcccacat cgtgctcagc
      481 tggtcgtcca ccgtccagac cccgaccgcc ccgccggcgg cgcggatctc ctccgccatc
      541 tcggggtcga gcgtcgcgta gtaggaggag aagaggtcgg cgccggatgc ccgcagcatc
      601 gccaccgggt cgatcggccg ggaggcgtcg atgatcccga ggtcgagctc gggcgccacc
      661 tccttcaaat ggcggatgca gcgatggtgg aaggagatca cgctcacccg ctcgaccatg
      721 tcgttctcgc gcagcacggc tgccagcttc tcctccaggc ccgggtaggg aaccgggttc
      781 tgcttggtct cgatcaccag cccgacccgg tcgcggacca gctcgaagag ctcctccagc
      841 agggggatct tctgaccctt gcccgcatca agctcgcgca gctcggccag cgtgtgggcg
      901 ccgaccaatc cggttccgtt ggtggtccgc tcgagcaggt gatcgtggat caccgccagc
      961 ttgccgtcgg ccgtcaggtg cacgtcgcac tcgacgaggt cgcagccggc gtcgatcgcc
     1021 gaccggaagg aggccagcgt gttctcggga tgctcggccg ggttgccacg atgaccgccc
     1081 agcatgatcc gcccctctgc cctggcggcc tccaaacgct gccgcatcgt gtaggggccc
     1141 tgcatcgaat ggtgagtcta aactcctgct caatgtcctc caccgagcct cgaacgatcg
     1201 ccgagctgcg cgcgtccggc caccagcgag agaccgtcaa gcaggagatg cgccgcaacc
     1261 tgctcgcccg ggtgcgcgag cgccggccga tctttcccgg catcgtcgga tacgaggaga
     1321 cggtaatccc gcaggtatcc aacgccgtaa tctccggcca cgacctgatc ttcctgggcg
     1381 agcgcggcca ggccaagagc cggctgatgc ggtcgatgac cgagctcctc gactccgagg
     1441 tgccggtgat ggccggctgc gagatcaacg accatccctt cgccccgatc tgcaagcgct
     1501 gccgagacga ggtcgaggcc agtggcgagg aggcggcgat
//
LOCUS       DATCQR010000243         1593 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_199022, whole genome shotgun sequence.
ACCESSION   DATCQR010000243 DATCQR010000000
VERSION     DATCQR010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1593
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199022"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1593
                     /locus_tag="VIN32_08095"
     CDS             <1..>1593
                     /locus_tag="VIN32_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HEY8922573.1"
                     /translation="IKIRLTERQRVIPAVAASAALLAMEYVDLWATSTDSAQPHRRRS
                     DVVEAAARQGRDLTKLANDFRDRLALIRDVGLTSGDTLAFSRAVRTHLDRFVIEEGIN
                     LNSAEKSALADIILRAAVGYGPLDSLLHDRSVSEIMVNGPDQVFIERRGVLTKADIRF
                     EDENQLLETIRRMVATTGRHIDGLNPMVDARLPDGSRVNAIIRPAAIHGAALTIRKFK
                     DAVLGIDDLTREGSLSPAMAEFLKAAVLGRMNVLVSGGTGSGKTTTLNVIARFIPHNQ
                     RVITIEDAAELQIDHPHVIALEHRPANVEGKGELTIRQLLRNSLRMRPDRILVGEVRG
                     SEALDMLQAMNTGHDGSMSTIHSNSARDALSRLETMVMMASIDIPFEAVRAQIASAVN
                     LIVHQARMPDGRRKVAQIAEVVGYDSNGAILRDIFLLGMGADLRLEYNATGYIPTSLD
                     KAAFYGVQVNQDLFDPVKSRFVPAGSDSMMPVVNDPLMSGQRGGGENVVRQVVVVPFS
                     SDRPGDGRQQQHSVQSTPSTPAT"
BASE COUNT          289 a          567 c          475 g          262 t
ORIGIN      
        1 atcaagatcc ggctgaccga gcgccagcgc gtgatccccg ccgtagccgc ctcggctgcg
       61 ttgctcgcca tggagtatgt cgacctgtgg gccacttcca ccgactcggc tcagcctcac
      121 cgccgccgct ccgacgtcgt cgaggctgca gcgcggcagg ggcgcgacct gaccaagctc
      181 gccaacgatt tccgcgatcg gctggcgctg atccgcgacg tcggactgac cagcggcgac
      241 accctcgcgt tctcgcgtgc cgtgcgcacg catctcgacc gtttcgtcat cgaagagggc
      301 atcaacctga actcggcaga aaaatccgcg ctcgccgaca tcatcctgcg agccgccgtc
      361 gggtacggcc cactcgacag ccttctccac gaccgctccg tgagcgagat catggtcaac
      421 ggccccgacc aggtattcat cgaacgccgc ggcgttttga ccaaggccga catccgcttc
      481 gaggatgaga accagctgct ggagacgatc cggcgcatgg tcgcgaccac cggccgccac
      541 atcgacggcc tcaacccgat ggtcgacgca cggctccccg atggcagccg cgtgaacgcg
      601 atcatcagac ccgctgcaat ccatggcgcc gcgctgacga tcaggaagtt caaggatgcg
      661 gtcctcggta tcgacgacct cacgcgggag ggcagcctca gccctgcgat ggccgagttc
      721 ctgaaagcgg cggtgctcgg ccgcatgaac gtgcttgtct ccggtggcac agggtctggc
      781 aagaccacga ccctcaacgt catcgcgcgc ttcatccctc acaaccagcg cgtgatcacg
      841 atcgaggacg ccgccgagct gcagatcgac caccctcacg tcatcgcgct cgagcatcgt
      901 cctgccaacg tcgaaggcaa gggcgagttg accatccgcc agctccttcg caacagcctc
      961 cggatgcggc ccgatcgcat ccttgtcgga gaggttcgtg gctccgaggc cctcgacatg
     1021 cttcaggcga tgaacaccgg ccacgacggc agcatgtcga cgattcactc gaactccgcg
     1081 cgcgacgccc tgtcccgcct cgagaccatg gtgatgatgg cctcgatcga catccccttc
     1141 gaggcggtgc gggcacagat tgcatcggca gtcaacctca tcgtgcacca ggctcgaatg
     1201 cctgacggcc gccgcaaggt cgcgcagatc gctgaggttg tcggttacga ctccaacggc
     1261 gcgatccttc gggacatctt cctgctcggc atgggcgcgg acctccgcct cgagtacaac
     1321 gcgacgggat acatcccgac ctcgctcgac aaggcagcct tctatggagt ccaggtcaac
     1381 caggaccttt tcgatcctgt caagtcccgc ttcgtcccgg cagggtcgga cagcatgatg
     1441 ccagtcgtca atgacccgct gatgagcggg cagcgcggcg gcggcgagaa tgtcgtccgc
     1501 caggtggtgg tggtgccctt cagctccgac cgccccggcg acgggaggca gcagcagcat
     1561 tcggtgcaat ccacgccctc gactccggcc acc
//
LOCUS       DATCQR010000244        10982 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_137283, whole genome shotgun sequence.
ACCESSION   DATCQR010000244 DATCQR010000000
VERSION     DATCQR010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 10982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10982
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137283"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1073)
                     /gene="tgt"
                     /locus_tag="VIN32_08100"
     CDS             complement(<1..1073)
                     /gene="tgt"
                     /locus_tag="VIN32_08100"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018086390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEY8922574.1"
                     /translation="MSAISFTSRATPIGRARLGSLTTPHGVIETPQFMPVGTQATVKA
                     LSPGDLHAAGVQVILANTYHLSLRPGHERIARLGGLHRFMGWDGPILTDSGGFQVFSL
                     AHLGKVDDDGVTFASHLDGSPQRLTPERAVEIQEALGSDIAMAFDQLVDATLPAAEIA
                     IAMQRTHRWAERCIDAHTRVDQALFGIVQGGVEADLRRESVASISALPFDGIAIGGLS
                     VGESKVQMKHTLEVVAEALADDPRVRYLMGVGSPPDFFIAVERGVDLFDCVLPTRVAR
                     TGQLWTSTGRLNLRNARFLDDPDPPDSDCRCEACRNHSRAYLAHLFRAEELLAYRLSS
                     LHNVTYTQDLMRRIRQALDDGSYG"
     gene            complement(1070..2167)
                     /gene="queA"
                     /locus_tag="VIN32_08105"
     CDS             complement(1070..2167)
                     /gene="queA"
                     /locus_tag="VIN32_08105"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009348744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEY8922575.1"
                     /translation="MTRSEPAARLRIADFDYELPPSAIAQVPIEPRDAARLLVLDPGA
                     SSPGRPALSHRVFGALGELLRPGDLLVVNDSRVMRARLSATRPGGGAAEVLLLRLLNG
                     TANRWEALVRPSRRIAPGAVITLRNGDLVEVGERLADGTRAVHFARDAHVVMAAVGET
                     PLPPYIRDRSSSPGRYQTVYADPPGSAAAPTAGLHFTAELLRSLEAAGVGHAHVTLHV
                     GLDTFRPLEGTFVDEHHIHREWYEIPHATEVAIARARREGGRVVAVGTTSVRVLESAG
                     RGRRSSGWTDLYITPPHEFSEVDALVTNFHLPRSSLLVLITAFVQAGMHAANPLEARD
                     TLLAAYGTALAQGYRFFSFGDAMFIGRTPAE"
     gene            complement(2232..3617)
                     /locus_tag="VIN32_08110"
     CDS             complement(2232..3617)
                     /locus_tag="VIN32_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEY8922576.1"
                     /translation="MDATAQPSTRRDEPPETGLAGYFHFRERGTNFGTEVRAGVTTFM
                     VMAYIIFLNPGILSAAGLDPVAGAAATALVAGIMTIAMGIVGNYPLALAAGLGINAVV
                     AFTLVLGKGLTLPGAMGVIVLEGIVVTLLVLVGLREAIMTAVPLALKRAIGVGIGLFI
                     LFIGFVNGGAIASPEGGVPPVVPVFPTEPAQFVFWAGLGLTIFLWVLKIRGALIISII
                     ATTVLALVVGVAKIPADFVLAPGANTFSTIGKFDLGDVFSKLGLLTALLTIFAIMLTD
                     FFDTMGTVTGVAAEAGLANEDGSVPGIGRVLLVDSVAAIAGGAAGISSNTTYIESAAG
                     VAEGGRTGVTSIVTGLLFLAAIFLSPLAGIVPAQATAPALVIVGYLMFTLISDINVRD
                     MEDGIPALLTMILMPLTYDITVGIGAGFISWVAIKVVKGKMGEVHLLMWLVAAAFVVF
                     YAKDWIATFIK"
     gene            complement(3734..3949)
                     /locus_tag="VIN32_08115"
     CDS             complement(3734..3949)
                     /locus_tag="VIN32_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020564284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HEY8922577.1"
                     /translation="MSPELGRFLVVIGILLVVVGGLAMAGIQIPFGRLPGDISITGEH
                     GGLFVPLGTMLVVSIVLSLLFNLFVRR"
     gene            complement(3954..4988)
                     /gene="ruvB"
                     /locus_tag="VIN32_08120"
     CDS             complement(3954..4988)
                     /gene="ruvB"
                     /locus_tag="VIN32_08120"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEY8922578.1"
                     /translation="MTIERIASAVVAEDEIALESTVRPRRLDEFAGQDRIKENLAILI
                     DAARHRDEPVDHILLYGPPGLGKTTLANIVAAEMGAQIRTTSGPAIERAGDLAAVLTA
                     LNKGDVLFIDEIHRLSHVVEEILYPAMEDFALDVVLGKGPGARTVRLQIENITVIGAT
                     TRVGSITGPLRDRFGVTYRLDYYTTDDLERILVRSAHILDVALDPAAAAIIAGRSRGT
                     PRIANRLLKRTRDFAQVRGDGIVTAELATDALDLLEIDDRGLDALDRQLLRAIAEHHG
                     GGPVGLQTMAATISEPVETLEDVIEPYLMQIGLLARTARGRQLTPGAWEHLGMTPPAR
                     SPEAQPGLFD"
     gene            complement(4999..5580)
                     /locus_tag="VIN32_08125"
     CDS             complement(4999..5580)
                     /locus_tag="VIN32_08125"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEY8922579.1"
                     /translation="MQGDRSFGAVVLAAGTSRRFGSPKQLAVVDGRTLLEHVIGAARA
                     AHLEPVVVVVPVWLSPPAGLDDKSVHWVRNPFPERGMSLSLRLGLGALGPDTSAAVIL
                     LGDQPGMRAATIMAVTRARGNRPMVAASAGGVLAPPVLIERTHFHLASGLTGDVGLRD
                     LLRASRDLVTTVPVTEHAVDIDTPEDLGRMGAP"
     gene            5602..6069
                     /locus_tag="VIN32_08130"
     CDS             5602..6069
                     /locus_tag="VIN32_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009179683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY8922580.1"
                     /translation="MAPMKARYGHTNLVARDWRQLAGFYIDLFGCEPVPPERDYRGPV
                     AESASGVPGSGWRGVHLRLPGHGDEGPTLEIYAYDREEPALPPAANRPGWGHIAFAVE
                     DVPAAQREVMTAGGSAVGEVITVETADGRQVTWCYVTDPEGNIVELQAWSDGG"
     gene            6066..7670
                     /locus_tag="VIN32_08135"
     CDS             6066..7670
                     /locus_tag="VIN32_08135"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY8922581.1"
                     /translation="MIDPPAVDLLVTGGSILDGSGAPAHQGTVASIDGRLHVADAEWR
                     PGHAARTVDASGMVVAPGFIDLHSHGGLVILADPRHEPKVRQGVTTEVIGVDGNGYAP
                     FADHEDLAAFVDLNAGLDGRPEIAYDWDMVASYIGRYDRGQAVNVAVVIGNSALRIGA
                     IGWDDTPADDRTLARMRAMLREGLEEGAFGVSSGLDYPPGAFASTDELAALTAEAASA
                     GGFYHTHVRYALGDRFLDPFREAIEIGRRGDGPVHLTHFYHRQAHPGGPEQLLALVDD
                     ARDAGMDVTFDTYPYEWASTRLLIQLPQWVQAGGPIALKERIADHAVRDRVRAELASR
                     GTAYTGSAGWADLRLGAFTQPDNLQWESRTLAEVMHETGSDPVDAICDLLLSENLGVA
                     QVTSGPWSETLPHFVRHPVGMVGTDSTFLGDKPSPRTYGSFPRILGQFVRDEQLLSLE
                     EAVRKMTSAPAARLGLTERGLLRDGFWADLVVFDPARVRSNATYDQPRQFPDGIEFVA
                     VNGELVVDGGVHTGALPGRALRRGRS"
     gene            7705..10023
                     /locus_tag="VIN32_08140"
     CDS             7705..10023
                     /locus_tag="VIN32_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEY8922582.1"
                     /translation="MTRILPPGTREIGAPDALVVPPGSATPGRIATRGPLRREGPAKL
                     TGLAQYADDLVFPGAWYGATIRSIEPHATLLSLDLDDAFDWGRVVVVTAADIPGDNVV
                     SLIRDDQPILVPVGGEIRHQAEALCLLAAADRETLRAAKRHMRVRTERLPATFDPLES
                     TEEFSHLEVRQGDPGAGFAEAELVIEGTYRVGHQEQLYIENQAMIAVPQPDGGVTVHG
                     SCQCPYYIHRALKRALQLTDTEAIVIQAETGGGFGGKEEYPSMIALHAALLARKLGKP
                     VRMIYDRHEDLSATTKRHPAVIHYRTGVMRDGRLVVQEIDVVMDGGAYCTLTPVVLSR
                     GVLHAGGPYRCPNVRIKGRAMATNTPPNGAFRGFGAPQTEFAAEMQVNRIAEALAMSP
                     LELRRLWAYRDGDTTPTGQVLRESVAAIEVLEAAAKASDYARHSEQTRRAREARLPGA
                     RLGSGIGMAMGWHGAGFTGSGEVHIAGTASVELTDAGTVRVLTAQTEMGQGTKTIFPQ
                     LVAGELGLEPEAVETAPQDTSIVPDSGPTVASRTTMVVGGLLVNASRRLRAQVEAVTD
                     QPFADSYRDFALEHGSLRIDERFEPYPNVQFDDETYTGDAYPAFGWACAVARVDVDLD
                     TGEVTVRDVVSADDAGRIVHPVLAEGQVEGGTLQAIGYATIEEIKLVDGRYLNDRLAT
                     YLIPTALDAPRIEAILVEKPFSGAPHGAKGIGELPMDVGAPAVVAAIHDATGVWIHDL
                     PATPERILAALTGAPTPGSPGVSEPAPERGAT"
     gene            10020..10502
                     /locus_tag="VIN32_08145"
     CDS             10020..10502
                     /locus_tag="VIN32_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEY8922583.1"
                     /translation="MSYRFLVNGDPVEVEVPGMRRLLDVLREDLALTGTKEGCGEGEC
                     GACSVLLDGAVVDACLVPICQVDGTSVRTVESLGTQEQLNDLQAAFLETGGAQCGICT
                     PGMLMAAEAFLATGGPATDDAIREAIAGNLCRCTGYTKIVEAIAQAADRRRTTGFGPA
                     "
     gene            10507..>10982
                     /locus_tag="VIN32_08150"
     CDS             10507..>10982
                     /locus_tag="VIN32_08150"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HEY8922584.1"
                     /translation="MPIEPPVASPHSLTEAYALLAEEPHRPVAGGTDLLVQITGELGE
                     PPERVLDIWHLDELRGIRMESNALMLGALTTYSEMRRSPLIAEFLPAFAEAAATIGAA
                     QIQNRGTIGGNAMNASPAGDMLPLLLACDAQFVAGAAHGERWIPVRDFWPSYRHTAL"
BASE COUNT         1755 a         3736 c         3882 g         1609 t
ORIGIN      
        1 ccgtacgacc cgtcgtcgag ggcctggcgg atgcgccgca tgaggtcctg agtataggtg
       61 acgttatgga gcgaggagag ccgataggcg agcagctcct cggcgcggaa caggtgggcg
      121 aggtaggccc gcgaatgatt gcggcatgcc tcgcagcggc aatcgctgtc cggcggatcg
      181 gggtcgtcga ggaagcgggc attgcgcagg ttgagcctgc cagtgcttgt ccagagctgt
      241 ccggtgcggg ccacgcgggt cggcaggacg caatcgaaga ggtcgacccc gcgttcgact
      301 gcgatgaaga agtccggcgg cgagcccacc cccatcaggt agcgcacgcg cgggtcgtcg
      361 gcgagcgcct cggccaccac ctcgagcgtg tgcttcatct gcaccttgct ctccccgacc
      421 gacaggccgc cgatcgcgat gccgtcgaac ggcagcgcgc tgatcgaggc gacactctcc
      481 cgacggagat cggcttccac cccgccctgc acgatcccga acaaggcctg gtccacccgc
      541 gtatgggcat cgatgcatcg ttcggcccac cggtgtgtgc gttgcatggc gatggcgatc
      601 tcggcggccg gcagcgtcgc gtccaccaac tggtcgaagg ccatggcgat atcgctcccc
      661 agcgcctcct ggatctcgac cgcacgttcg ggcgtcaggc gttgagggga tccgtcgagg
      721 tggctggcga aggtcacgcc gtcgtcgtcc accttgccga ggtgagcgag gctgaacacc
      781 tggaacccgc ctgaatcggt gaggatcggc ccatcccacc ccatgaaccg atgcagcccg
      841 ccaaggcgcg cgatccgctc gtggccgggc cgcagcgaga ggtggtaggt gttggccagg
      901 atgacctgga cgcctgccgc gtgaaggtcc cccggcgaca gcgccttgac cgtggcctgg
      961 gtaccgaccg gcatgaactg cggggtctcg atgacgccgt gcggggtggt cagcgaaccc
     1021 agccgcgccc gtccgatcgg cgtcgcacgc gaggtgaatg agatcgcgct cactcggctg
     1081 gcgtccggcc gatgaacatg gcgtcgccga agctgaagaa ccgataaccc tgtgccaacg
     1141 cggtgccata ggcggccagg agggtgtcac gggcctcgag cggatttgca gcgtgcatgc
     1201 cagcctggac gaacgcagtg atgagcacca gcagtgagct ccgcggcagg tggaagttgg
     1261 tgaccagggc gtcgacctcg ctgaactcgt ggggaggcgt gatgtagagg tcggtccaac
     1321 ccgagctgcg ccggccacgc ccggcactct ccaggacacg cacgctggtc gttcccaccg
     1381 cgaccactcg gccgccttcc cgtctggccc gggcgatggc gacttccgtt gcgtgcggga
     1441 tctcgtacca ctctcgatgg atgtgatgct cgtcgacgaa tgtcccctcg agtggtcgaa
     1501 aggtatccag ccccacgtgc agcgtgacgt gtgcgtggcc cacccccgcg gcctccagcg
     1561 atcggagcag ctcggctgtg aagtggagcc cggcggttgg ggcggccgcg gagccgggag
     1621 gatcggcgta caccgtctgg tagcggcccg gggagctgga tcggtcgcgg atgtagggcg
     1681 gcagtggcgt ctccccgacc gcggccatca ccacgtgcgc gtcgcgggcg aagtgcaccg
     1741 cccgcgttcc gtctgcaaga cgctccccca cctcgacaag gtcaccgttg cgcagcgtga
     1801 tcaccgcgcc gggtgcgatc cgtcgtgacg ggcggacgag tgcctcccac cggttcgcgg
     1861 tcccgttcag caggcgcagc aacaggacct cggctgcgcc gccgccgggc cgcgttgcgg
     1921 acagccgcgc ccgcatgacg cgcgaatcgt tgaccaccag gaggtcgccg gggcgtagga
     1981 gctctccgag cgcgccgaag acgcggtgcg agagcgccgg acggcccggc gacgatgccc
     2041 ccggatccag caccagcagg cgtgcggcgt cgcgcggctc gatcgggacc tgcgcgatcg
     2101 ccgatggcgg cagctcgtag tcgaagtcgg cgatccgcag tcgcgcggca ggttcggaac
     2161 gggtcatcgg cccggacagc gtagcgcccg gccgggttgc ccggccgggc gccatcggac
     2221 tcgacaggac gctacttgat gaaggtcgcg atccagtcct tcgcatagaa gaccacgaag
     2281 gccgcggcga cgagccacat caggagatgg acctcgccca tcttgccctt gacgaccttg
     2341 atcgccaccc agctgatgaa gccggcgccg atgccgacgg tgatgtcgta ggtgagcggc
     2401 atcaggatca tcgtgaggag ggcgggaatg ccgtcctcca tgtcgcgcac attgatgtcg
     2461 ctgatcagcg tgaacatcag gtaaccgacg atcaccaggg cgggagccgt cgcctgggcc
     2521 ggcacaatgc cagccaacgg cgagaggaag atcgccgcca gaaagaggag gccggtcacg
     2581 atcgacgtga cgcctgttcg gccgccctcg gcgacgcccg ctgcgctttc gatgtaggtg
     2641 gtgttcgagg agatgcccgc ggcacctccg gcgatggccg ccacgctgtc aacgaggagg
     2701 acccgcccga tccccggcac cgagccatcc tcgttcgcaa ggccagcctc ggccgccacg
     2761 ccggtgacgg tgcccatcgt gtcgaagaag tcggtgagca tgatcgcgaa aatggtgagg
     2821 agtgccgtca gcaggccgag cttgctgaag acgtcgccca ggtcgaactt gccgatcgtg
     2881 ctgaaggtgt tcgcgcccgg agcgagtacg aagtcggcgg ggatcttcgc gacgcccacc
     2941 accagggcca gcacggtcgt ggcgatgatg ctgatgatca gcgcgccgcg gatcttcagc
     3001 acccacagga agatggtgag gccgaggccg gcccagaaga cgaactgcgc aggctcggtc
     3061 gggaacaccg gcacgactgg cggcacgccg ccctccggcg acgcgatcgc cccgccgttc
     3121 acgaagccga tgaagaggat gaagagtccg atgccgaccc cgatcgcccg cttcagggcg
     3181 agcggcactg cggtcatgat cgcttcacgg aggccgacca ggacaagcag cgtgacgacg
     3241 atgccctcga gcacgatcac gcccatggcg cctggaaggg tgagtccctt cccgaggaca
     3301 agggtgaagg cgaccaccgc attgatgccg aggccagcgg ccagggccag cgggtagttg
     3361 ccgacgatcc ccatggcgat ggtcatgatc ccggcgacca gtgccgtggc tgcggctccc
     3421 gcgaccgggt cgagtcccgc agccgacaga atcccggggt tcaggaagat gatgtaggcc
     3481 atcaccatga aggtggtgac gccggcacgt acctccgtcc cgaaattcgt cccccgttcc
     3541 ctgaagtgga agtagccggc gagccccgtc tctggtggct cgtcacggcg ggtgctgggc
     3601 tgggcggtcg cgtccactct gtgaacctcc tccgtacggg cccaccgggc ggagccggcg
     3661 gtggccagcg cggatcgtag tcctggctgc gccgccgcga ggggcatttc cgcgtgtgct
     3721 gacggccggc tggtcagcgc cgcacgaaga ggttgaagag aaggctcagg acgatcgaga
     3781 cgacgagcat cgtgccgagc ggaacgaaga gtccgccgtg ctcgccggtg atcgagatgt
     3841 ctcccggcag gcgtccgaat gggatttgaa tgccggccat cgctaggccg ccgactacga
     3901 ccagcaggat gccgatcacg accaggaagc ggccgagctc gggggacatc ggcctagtcg
     3961 aagagccctg gctgcgcctc gggcgagcgc gccggcggcg tcatgccgag gtgctcccac
     4021 gccccgggcg tcagctgccg gccgcgcgcg gtgcgcgcca gcagcccgat ctgcatcagg
     4081 tacggctcga tcacgtcctc gagcgtctcg accggctcgg agatggtggc ggccatcgtc
     4141 tgcaggccga ccggcccgcc gccgtgatgc tcggcgatgg cgcgcaggag ctgccggtcg
     4201 agcgcgtcga gcccacgatc gtcgatctcg agcaggtcga gggcatcggt cgccagctcg
     4261 gccgtgacga tgccatcgcc tcgcacctgg gcgaagtcgc gtgtccgctt caggagtcga
     4321 ttggcgatgc gcggcgtgcc gcggctgcgg ccggcgatga tcgccgcggc ggcaggatcg
     4381 agtgccacgt ccaagatgtg ggcgctccgg acgaggatcc gctccaggtc gtccgtggtg
     4441 tagtagtcga ggcgataggt caccccgaag cgatcgcgca gcggcccggt gatgctcccc
     4501 acccgtgtcg tggccccgat cacggtgatg ttctcgatct ggagccggac ggtgcgggcg
     4561 ccggggccct tgccaaggac cacgtccagg gcgaagtcct ccatcgccgg gtacaggatc
     4621 tcctcgacca cgtgggagag gcgatggatc tcgtcgatga agagcacgtc gcccttgttc
     4681 agcgcggtga gcaccgcggc caggtcgccg gcgcgctcga tggccggacc ggaggtagtc
     4741 cggatctggg cgcccatctc ggccgccacg atgttggcga gggtcgtctt gcccaggccg
     4801 ggcggtccgt agaggaggat gtgatcgacc ggctcatcgc ggtggcgtgc ggcgtcgatc
     4861 aggatcgcca ggttctcctt gatgcggtcc tgtccggcga actcgtcgag ccgccttggc
     4921 cgcaccgtcg actcgagcgc gatctcatcc tcggcgacga cagcgctggc gatccgctcg
     4981 atggtcacgt cggccattct agggggcgcc catgcgacca aggtcctccg gggtgtcgat
     5041 gtccaccgcg tgctcggtga ccggcactgt cgtgacgagg tcccggctcg cgcgcagcag
     5101 gtctcggagc ccgacatcgc cggtcaggcc agaggcgagg tggaagtgcg tccgctcgat
     5161 gagcaccggt ggcgcaagga cgccgcccgc ggacgcggcg accatcgggc gattacccct
     5221 cgcacgcgtc accgccatga tcgtggccgc ccgcatgccc ggctggtcgc ccagcaggat
     5281 cactgccgcc gaggtgtccg gcccaagcgc cccgaggcca aggcgcagcg aaaggctcat
     5341 gccgcgctcc gggaaggggt tccggaccca gtgcacggac ttgtcgtcga gaccggcggg
     5401 aggcgacagc cacaccggca caaccacgac cacgggctcc aggtgagcag cccttgcggc
     5461 gccgatgaca tgctcgagca gggtgcgacc gtcgacgacc gccagctgct tcgggctgcc
     5521 aaagcgtcgt gacgtgccgg ccgccaggac cactgcgccg aatgatcggt ctccctgcat
     5581 ccggctatta gactgcccca catggccccg atgaaggcgc gatacgggca caccaacctg
     5641 gtggcacgag attggcggca gcttgcgggc ttctacatcg acctcttcgg gtgcgaaccg
     5701 gtcccacccg agcgcgacta ccgcggtccg gtcgccgagt cggcgtccgg cgttccgggg
     5761 tcgggatggc ggggcgtgca cctccgcctg ccaggtcacg gcgacgaagg accgacgctc
     5821 gagatctacg cctacgaccg ggaggagccc gcgctccctc ccgctgccaa tcgccccggc
     5881 tggggccata tcgccttcgc ggtcgaggat gtccccgccg cacagcgaga ggtcatgaca
     5941 gccggcgggt ccgccgtcgg cgaggtgatc accgtcgaga cggccgatgg gaggcaggtg
     6001 acgtggtgct acgtcaccga tccggagggg aacatcgtcg agctccaggc ctggagcgac
     6061 ggcgggtgat cgacccgccg gccgtcgacc tgctggtcac gggaggctcc attctcgacg
     6121 gcagcggggc tccggctcac caagggacag tggcgagcat cgacgggcgc ctgcacgtgg
     6181 ccgatgccga atggcgcccc ggccacgcgg cgcggacggt ggatgccagc gggatggtcg
     6241 tcgcgcccgg gttcatcgac ctgcactcgc acggcggcct ggtgatcctg gccgatccgc
     6301 gccacgagcc caaggtgcgg cagggcgtca ccaccgaggt gataggggtc gacggcaatg
     6361 gctacgcccc gttcgccgac cacgaggacc tggccgcgtt cgtcgacctc aacgccggcc
     6421 tcgacggccg cccggagatc gcctacgact gggacatggt cgcgagctac ataggtcgct
     6481 acgaccgggg ccaggccgtg aacgtggccg tcgtgatcgg gaacagcgcg ctacgtatcg
     6541 gcgccatcgg ctgggacgac actccggccg atgaccgcac cctcgcccgc atgcgcgcca
     6601 tgcttcgcga aggcctggaa gagggagcct tcggggtctc gagcgggctc gactacccac
     6661 ccggcgcgtt cgcttcgacc gatgagctgg ccgcactcac cgccgaggcg gcatcggcgg
     6721 gcgggttcta tcacacgcac gttcgctatg cgctcggcga ccgcttcctc gacccgttcc
     6781 gcgaggcgat cgagatcggg cgcaggggcg acgggccggt tcacctgacc catttctatc
     6841 accggcaagc tcaccccggg ggtcccgagc agctgcttgc cctggtcgac gacgcccgcg
     6901 acgctggaat ggacgtcacc ttcgacacgt acccatacga gtgggcctcg acccggctgc
     6961 tgatccagct tccacagtgg gtccaggcgg gagggccgat cgcgctcaag gagcgcatcg
     7021 ccgatcacgc ggtgcgcgat cgcgtgcggg cagagctcgc atcgcgcggc acggcctaca
     7081 cagggtcggc cggctgggcc gatctgcgcc tcggcgcctt cacccagccc gacaacctgc
     7141 aatgggagag ccgcacccta gccgaggtca tgcacgagac cggcagcgat ccggtcgacg
     7201 cgatctgcga cctgctgctg tccgagaatc tcggtgtagc gcaggtcacc agcgggccgt
     7261 ggagcgagac gctcccccac tttgtgcgcc acccggtcgg catggtcggc accgactcca
     7321 ccttcctggg cgataagcct tcgccacgga cgtacggctc cttcccgagg atccttggac
     7381 aattcgtgcg tgacgagcag ctcctctcgc tggaagaggc ggtgcgcaag atgacgagcg
     7441 cgcccgcggc gcggctgggg ctcacagagc gaggcctgct gcgcgacggc ttctgggccg
     7501 acctggtggt cttcgacccc gcccgcgtcc gctcgaacgc gacctatgac caaccgcgcc
     7561 agtttcccga cgggattgaa ttcgtagcgg tcaatgggga gctcgtggtg gatggcggtg
     7621 tgcatacggg cgcattgccc gggcgcgcgc tgcgccgggg ccgctcgtga cggtgctatc
     7681 gtccgcccag gaggagcgag ccggatgacg cgcattctgc cccccggaac ccgcgagatc
     7741 ggcgccccgg acgcgctcgt cgtgccgccg ggaagtgcga cgccggggcg catcgcgact
     7801 cggggcccgc tccgtcgcga ggggccggcc aagctcaccg ggctcgcgca atacgctgac
     7861 gatctcgtct tccccggcgc ctggtacgga gccaccatcc gctcgatcga gccgcacgcg
     7921 acgctgctct cgctggacct tgacgatgcg ttcgactggg gccgggttgt cgtcgtcacc
     7981 gcggcggaca tccccggcga caacgtcgtg agcctgatcc gtgacgacca gccgatcctg
     8041 gtaccagtcg gcggtgagat ccgccaccag gcggaggcgc tctgcctgct cgctgctgcc
     8101 gaccgcgaaa cgctgcgagc cgcgaagcgc catatgcgcg tgcggacgga acggctgccg
     8161 gcgaccttcg acccgcttga gtcaaccgag gagttctccc acctcgaggt gcggcagggc
     8221 gacccgggtg cgggcttcgc cgaggcggag ctcgtgatcg agggcacgta ccgggtcggc
     8281 caccaggagc agctctacat cgagaaccag gcgatgatcg ccgttcccca gccggacgga
     8341 ggggtgacgg tgcacggctc atgccagtgc ccgtactaca tccaccgcgc cctgaagcga
     8401 gcgctccagc tgaccgatac ggaggcgatc gtgatccagg ccgagacggg cggcggcttc
     8461 ggtggcaagg aggagtaccc gtcgatgatc gccctgcatg cggcgttgct cgcgcgcaag
     8521 cttggcaagc ccgtgcgcat gatctacgac cgccacgagg acctgagcgc gacaaccaag
     8581 cgtcacccag cggtcatcca ctaccgcact ggggtgatgc gcgacggtcg gctggtcgtc
     8641 caggagatcg atgtcgtgat ggacggcggc gcgtactgca ccctcacgcc cgtcgtgctg
     8701 tcgcgcggcg tcctgcacgc gggcggtccg tatcggtgcc cgaacgtccg gatcaagggt
     8761 cgtgccatgg ccaccaacac cccgccgaac ggagccttcc gcggctttgg ggctccacag
     8821 accgagttcg ccgccgagat gcaggtcaac cggatcgcag aggcgctcgc aatgagcccg
     8881 ctggagctgc gacgcctgtg ggcgtaccgc gacggcgaca ccacgccgac cggccaggtc
     8941 ctgcgcgaaa gcgtggccgc catcgaggtg ctcgaggcag ccgccaaggc cagcgactac
     9001 gcgcggcaca gcgagcagac gcgccgggcg cgggaggccc ggctgccagg cgcgcgcctc
     9061 ggatccggga tcgggatggc aatgggctgg cacggcgcgg ggttcaccgg gtctggcgaa
     9121 gtgcatatcg ccgggaccgc ctcggtcgag ctgacggacg ccggcacggt tcgcgtgctt
     9181 accgcccaga ccgagatggg ccaggggacc aagaccatct tcccgcagct ggttgcgggc
     9241 gagctgggcc tcgagcccga ggcggtggag actgcgccgc aggacaccag catcgtcccc
     9301 gactcaggcc cgaccgttgc ctcacgcacg acgatggtgg tcggcgggct gctggtgaat
     9361 gcttcacgcc gccttcgagc acaggtggag gccgtcaccg accaaccatt cgccgattcg
     9421 tatcgcgact tcgcgctcga gcacggctcc ctgcggatcg atgagcgctt cgagccatac
     9481 ccgaacgtgc agttcgatga cgagacctac acgggcgatg cctacccggc gttcgggtgg
     9541 gcctgcgcgg ttgctcgggt cgacgtggac ctggacaccg gcgaggtgac cgtccgcgac
     9601 gtcgtctcgg ccgatgacgc gggccggatc gtccaccccg tgctcgccga gggccaggtg
     9661 gagggcggga cgctgcaggc gatcggctac gcgactatcg aggagatcaa gctggtcgac
     9721 ggccgctacc tgaatgaccg gctggctacc tacctgatcc cgaccgcgct ggatgcgccc
     9781 cggatcgagg cgatcctggt cgagaagccc ttctcggggg ctccccacgg tgccaagggg
     9841 atcggcgagc tgccgatgga cgtgggcgcg ccggccgtgg tggctgccat ccacgacgcc
     9901 acgggcgtct ggatccacga cctgccggca accccggagc gcatcctggc ggcactgacc
     9961 ggcgcgccga cgccaggctc acccggcgtc agcgagccgg cgccggagcg aggcgcgacg
    10021 tgagctatcg gttcctggtc aacggcgacc cggtcgaggt cgaggtcccc ggaatgcgtc
    10081 gcctgctcga cgtgctgcgc gaggacctgg cgctgaccgg caccaaggaa ggctgcggcg
    10141 agggcgaatg cggagcgtgc agcgtgctgc tcgatggggc cgtggtcgac gcatgcctgg
    10201 tgccgatctg ccaggtcgac ggaacgagcg ttcggactgt ggagagcctt ggcacccagg
    10261 agcagctgaa cgacctgcag gctgccttcc tggaaactgg cggtgcccag tgcgggatct
    10321 gcacgcccgg gatgctgatg gccgccgagg cgttcctcgc gactggcggc ccggcaacgg
    10381 acgatgccat ccgcgaggcg attgcgggca acctgtgccg ctgcaccggg tacacgaaga
    10441 tcgtcgaggc catcgcgcag gccgcggacc gcaggcgcac aactggcttc ggtccggcct
    10501 gagccgatgc ccatcgagcc gccggtcgct tcgccgcaca gcctgaccga ggcctatgcc
    10561 ctgctcgccg aggaaccgca tcgaccggtc gcgggcggca ccgacctgct ggtccagatc
    10621 accggcgagc ttggagagcc gcccgagcgg gtgctcgaca tctggcacct tgacgagctg
    10681 cgcgggatcc ggatggagtc gaacgcccta atgcttggcg ccctgaccac ctactcggag
    10741 atgcggcgat cgccgctcat cgccgagttc ctgcctgcct ttgccgaggc cgccgcgacg
    10801 atcggggcgg cgcagatcca gaaccgcggc acgatcggcg gcaacgcgat gaacgcctcg
    10861 cccgcggggg acatgctgcc gctcctgctc gcctgcgacg cccaattcgt cgccggtgct
    10921 gcgcacggcg agcgctggat ccccgtccgc gacttctggc cgagctaccg gcataccgcg
    10981 ct
//
LOCUS       DATCQR010000245         4589 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_45182, whole genome shotgun sequence.
ACCESSION   DATCQR010000245 DATCQR010000000
VERSION     DATCQR010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4589
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45182"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(145..891)
                     /gene="pgl"
                     /locus_tag="VIN32_08155"
     CDS             complement(145..891)
                     /gene="pgl"
                     /locus_tag="VIN32_08155"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HEY8922585.1"
                     /translation="MTVRFRILPTPAALAEAAADRLAAAARNAIRRRGRCLLALSGGS
                     TPRLIYPLLVSRPRIDLVDWSRVEFFWGDERAVPPGSQDSNFGLARTLLLGHLPGVRP
                     AAVHRMPADAPDSVAAAERYEAEVGHAFGMQPGTSRRPRFDLIWLGMGPDGHTASLFP
                     GASTLAERRRWVLPATAPATSAVPGRMTFTLPLINAARAVMFVVGGADKARAIRSIRS
                     GSRDLPAARVRATSTLWLLDALAAGEAAPK"
     gene            complement(888..2183)
                     /locus_tag="VIN32_08160"
     CDS             complement(888..2183)
                     /locus_tag="VIN32_08160"
                     /inference="COORDINATES: protein motif:HMM:NF021632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase assembly
                     protein OpcA"
                     /protein_id="HEY8922586.1"
                     /translation="MATAVGRSTAARRALAVHRRLMRFGEAESTVAEPWVDRGTTVQA
                     IAGHLAQLWTSPPSSGDSTVTEKGMAHARASVLNLIVMVADEEAASRVVETMVGLGVR
                     HPSRAIVLSADPKAAGPTLNASITAHCNPGPEGAEPICFEVVVLSVHGDAADHLAGIV
                     APLLIHDLPTHIWWPGDPPFADPIFDQLVEIGDRVLVDTDEFSDLLHGLRRLTTLRRR
                     SGVGDLAWHRLAWWQELTAEFFDAPRFRRYLPNLNRLTIRYAVPPAHKPRRARKPRGD
                     LHAASDVASPLAGPMLYAGWIASRIEWRRYATTLPLKDGTLRLKLEGRHEMVDLQVEA
                     VETDALPPGELIAVRLRAFGEMGAAEFIIERSTDEAVVASNADGMTALLRRMPMQLPS
                     ESELLAADLVMDQHDPVYEAAVRAAAVFLASARQVDLAG"
     gene            complement(2168..3763)
                     /gene="zwf"
                     /locus_tag="VIN32_08165"
     CDS             complement(2168..3763)
                     /gene="zwf"
                     /locus_tag="VIN32_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEY8922587.1"
                     /translation="MTISSDIDEKAPSRPATARNPLRAGLRLQRMPEPCTMVIIGATG
                     DLTERKLAPALYNLMLGGTLPPEFTVVGFARRDLSADEFRAHLRSGIAKYSRNRPVKA
                     SIWESFSAGIEYQRGEFHDPIAWAALAKKLERIDRDRGTAGNRLFYLAVPPALHAEIV
                     RQIDAAGLSGTGNGTDPRYRGWSRVIVEKPFGFDLASARKLNRELREVFDEDQIFRID
                     HYLGKETVQNLSVFRFGNGLFEPIWNRRYIDSVQITVAETVGIEGRGESYDETGALRD
                     IVQNHVLQLMAVFAMEPPIEFKAADLRDEKVKVLRAVKPMSPTEVEASTIRGQYVSGW
                     VEGEKVPAYREEPEVAASSESETYVALKLAIESWRWAGVPFYVRTGKALATRVTEIAV
                     QFKRAPLALFSRAGAPPMEPNILAIRVQPDEGILLRFGAKVPGPGLQIRGVNMDFRYG
                     SSFAVDSPDAYETLLLDCMIGDASLFTRNDEVERAWEILDPILDAWASGEGGRLHFYP
                     AGSWGPAAADELVEHDGRAWRRP"
     gene            complement(3770..>4589)
                     /gene="gnd"
                     /locus_tag="VIN32_08170"
     CDS             complement(3770..>4589)
                     /gene="gnd"
                     /locus_tag="VIN32_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HEY8922588.1"
                     /translation="DLAAEPENGGHVTVAESLEALVALMSAPRHVAVSVPSGDATGEL
                     VDRLAKVLSPGDVIVDAGNSNFHDSQRRSTQLEAGGIEFLDMGVSGGIWGLQVGFCAM
                     IGGKREVFERFEPAVKSLAPEGGYLYCGPSGAGHYVKMVHNGIEYGLMQAYGEGFDIM
                     HASEFEIDLAAVSRLWNNGSVIRSWLLELAGDAFAKEGNDLADIRGFVADSGEGRWTV
                     ADAIEHDVPAPIITLSLLQRFRSRRDPDSFSDRVLAALRNEFGGHAVKSSDEGS"
BASE COUNT          729 a         1593 c         1586 g          681 t
ORIGIN      
        1 gctcaccggc gcgcccgcgc caggcgaggt agcgatccgt gttcaggtcc cgacggcggc
       61 gcgcggcgaa gccggtccag gtcggccatc cgacgaggta ggcggcgaca cctgcgatgg
      121 cagcgccgat cagccagagc caggtcactt gggagccgcc tcacccgctg cgagcgcatc
      181 cagcagccag agggttgacg ttgcccgaac gcgcgcggcg ggaagatcac gagacccgga
      241 gcgaatcgag cggatcgccc tggccttgtc cgctccccca accacgaaca tcacggcccg
      301 ggcggcgttg atgagcggga gcgtgaatgt catacgccct ggtaccgcgg aggtggcggg
      361 ggcggttgcc ggaagcaccc agcgccgtcg ctcggccagg gtactcgcgc ccgggaagag
      421 ggaggcggtg tgcccgtcgg gccccatccc cagccatatg agatcgaacc tgggccggcg
      481 gctcgtaccc ggctgcattc cgaaggcgtg gcccacctca gcctcgtacc gctcagcggc
      541 ggccacggag tcgggtgcgt cggccggcat gcggtgaacc gctgcgggac ggacgccggg
      601 aaggtggccg agcaggagcg tccgcgccag gccaaagttc gagtcctggc tgcccggcgg
      661 gacggcgcgc tcgtcgcccc agaagaactc cacgcgcgac cagtcgacca ggtcgatccg
      721 gggccgggac accagcaggg ggtagatgag gcgcggggtc gagcctcccg agagggcgag
      781 caggcaccga ccgcgacggc ggatcgcgtt gcgggccgcc gccgcgaggc ggtcggccgc
      841 cgcctcagcc aatgccgcgg gcgtgggcag gatcctgaac cgaacggtca ccccgccagg
      901 tcgacctgcc gggccgaggc gaggaagacg gcggccgcgc gaaccgcggc ctcgtacacg
      961 gggtcgtgct ggtccatcac caggtcggct gccagcagct cggactcgct cggcagctgc
     1021 atcggcatcc gacgcaggag ggcggtcatg ccatctgcat tggaggcgac gaccgcctcg
     1081 tcggtggatc gctcgatgat gaactcggcc gcgcccatct cgccgaacgc ccgcaggcgg
     1141 acggcgatca gctccccggg cggcagcgca tcggtctcaa ccgcctcgac ctgcaggtcg
     1201 accatttcgt ggcggccttc gagcttgagc cgcagagtgc catccttcag cggaagggtt
     1261 gtcgcgtagc ggcgccactc gatgcgcgag gcgatccaac ctgcgtagag catcgggccg
     1321 gcgagcggcg acgcaacgtc cgaggctgcg tggagatcgc ctcggggctt ccgtgcacgc
     1381 cgcggcttgt gcgccggcgg aaccgcgtag cgaatcgtca gccggttcag gttcggcagg
     1441 tagcgtcgga agcgcggggc gtcgaagaac tcggccgtca gctcctgcca ccacgccagc
     1501 cggtgccatg ccaggtcacc cacgccgctt cggcggcgga gggtggtcag ccgtcgcagc
     1561 ccgtgcagca gatcgctgaa ttcgtcggtg tccaccagca cgcggtcacc gatctcgacc
     1621 aactggtcga agatcgggtc ggcgaacggc ggatcgcccg gccaccagat gtgggtcggc
     1681 aggtcgtgga tcagcagcgg cgcgacgatc ccagcgaggt ggtcggcagc gtcgccatga
     1741 acgctgagca ccaccacctc gaagcagatc ggttcggccc cctcgggccc ggggttgcag
     1801 tgggcagtga tgctggcatt cagggtcgga ccggccgcct tcggatccgc ggacaggacg
     1861 atcgcgcgag atgggtgacg cacgccaagg ccgaccatcg tctcgacgac gcggcttgcc
     1921 gcctcctcat cagcgaccat cacgatcagg ttcagcaccg atgcgcgcgc gtgcgccatc
     1981 cccttctcgg tgaccgtcga atccccgctg ctgggcgggc tggtccacag ctgcgccaga
     2041 tggccggcga tggcctgcac cgtggtcccg cggtcgaccc agggctcggc caccgtggac
     2101 tcggcctcgc caaagcgcat caggcggcgg tggaccgcca gcgcccgtcg ggcggcggtc
     2161 gagcgcccta cggccgtcgc catgcccgtc catcgtgctc gaccagctcg tcagcggccg
     2221 ccggacccca gctgccagcg ggatagaagt gcagccgacc gccctcgcct gatgcccagg
     2281 catccaggat cggatccagg atctcccagg cgcgctccac ctcgtcgttg cgggtgaaga
     2341 gcgaggcgtc gccgatcatg caatcgagca ggagcgtctc gtatgcatcg ggcgagtcga
     2401 ccgcgaagct cgacccgtat cgaaagtcca tgttcacgcc acggatctgg agccccggac
     2461 ccggcacctt ggctccgaat cgcagcagga tcccctcgtc cggctggact cggatggcaa
     2521 ggatgttcgg ctccatgggt ggtgcgccgg cacgcgagaa gagcgcgagc ggcgcccgct
     2581 tgaactggac cgcgatctcg gtgacgcgcg tggcgagcgc cttgccggtc cgaacgtaga
     2641 acggcacacc ggcccagcgc cacgactcga tcgccagctt gagggcgacg tacgtctcgc
     2701 tctccgagct tgcggcgact tcgggctcct cccggtaggc ggggaccttc tccccttcga
     2761 cccagccgga cacgtactgg ccgcgaatgg tggatgcctc gacctcggtc ggggacatcg
     2821 gcttcaccgc gcgcagcacc ttcaccttct cgtcgcgcag gtcagccgct ttgaactcga
     2881 tcggcggctc catggcgaag accgccatca gctgcaggac gtggttctgc acgatgtcgc
     2941 gcagggcacc ggtctcgtcg tatgactcgc ctcgaccctc gatcccgacc gtctctgcga
     3001 cggtgatctg gacggagtcg atgtagcggc gattccagat cggctcgaac aggccgttcc
     3061 cgaaccggaa gaccgacagg ttctggaccg tctccttgcc caggtaatga tcgatacgga
     3121 agatctggtc ctcgtcgaag acctcccgca gctcccgatt gagcttcctg gcgctcgcca
     3181 ggtcgaagcc gaagggcttc tcgacgatga cccgggacca gccgcgatag cgcgggtcag
     3241 ttccattccc cgtgccggag agcccggcgg catcgatctg acgcacgatc tccgcatgca
     3301 gggcgggcgg caccgccagg tagaagaggc ggttccccgc cgttccccga tcgcgatcga
     3361 tccgctccag cttcttggcc agcgcagccc acgcgatcgg gtcgtggaac tcgccgcgct
     3421 ggtactcaat cccggcgctg aacgactccc agatcgaggc cttgaccggc cggttgcggc
     3481 tgtacttggc gatcccgctg cgcagatgcg cacggaactc gtcggcgctg aggtcacgcc
     3541 tcgcgaagcc gaccaccgtg aattcgggtg gcagggtgcc gcccagcatg aggttgtaga
     3601 gggcgggggc cagcttgcgc tcggtgaggt cgccggtcgc gccgatgatg accatggtgc
     3661 acggctccgg catccgctgg agccggaggc ccgctcgaag ggggttgcga gcggtcgccg
     3721 gccgcgaggg cgccttctcg tcgatgtcgc tgctgatggt catcgcgcgt caggagccct
     3781 catccgagga cttgacggcg tgtccgccga actcgttgcg cagggcagcc aggacgcgat
     3841 cgctgaagct gtccgggtcg cgccggctgc ggaatcgctg gagcagcgag agggtgatga
     3901 tcggcgccgg cacgtcatgc tcgatcgcat cggccaccgt ccagcgtccc tcgcctgaat
     3961 cggcgacaaa gccgcggata tcggccaggt cgttgccctc cttggcgaag gcgtcacctg
     4021 ccagctcgag cagccaggag cggatcaccg agccgttgtt ccagaggcgc gacacggcgg
     4081 cgaggtcgat ctcgaactcg gaggcgtgca tgatgtcgaa gccctcgccg tatgcctgca
     4141 tcaggccgta ctcaatcccg ttgtgcacca tcttcacgta gtgcccggcc cccgatggac
     4201 cgcagtacag gtagcccccc tccggtgcca gcgacttcac ggccggctcg aagcgctcga
     4261 agacctcgcg cttcccgccg atcatggcgc agaagccgac ctgcagcccc cagatgccgc
     4321 cgctgacccc catgtccagg aactcgatgc cacccgcctc gagctgggtg gagcgccgct
     4381 gcgagtcgtg gaagttgctg ttgccggcgt cgacgatcac gtcgccgggc gagagaacct
     4441 tggccaggcg atccaccagc tcgccggtcg cgtcgccgga aggaacgctg accgcgacat
     4501 gtcggggtgc gctcatgagc gcgacgagcg cctcgagcga ctccgcgacc gtcacgtggc
     4561 cgccgttctc cggctccgcc gccaggtcg
//
LOCUS       DATCQR010000246         3533 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_106283, whole genome shotgun sequence.
ACCESSION   DATCQR010000246 DATCQR010000000
VERSION     DATCQR010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3533
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106283"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(170..1774)
                     /locus_tag="VIN32_08175"
     CDS             complement(170..1774)
                     /locus_tag="VIN32_08175"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009192952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HEY8922589.1"
                     /translation="MKSTYDYIVVGAGSAGCVLAARLTEDAEVKVLLLEAGPPDSEPG
                     IHVPVAFSRLFKTPFDWDYSTEPEAHLDGRRIYHPRGRVLGGSSSINAMVYIRGNRAD
                     YDEWRDLGNPGWGYEDVLPYFKRAEDNERGASKYHGAGGPLSVSEGRSQNPLARAFVE
                     SAVAAGLPRNEDFNGQEQDGAGYYQVTQRNGRRCSAAVAYLHPAMSRPNLAVRTGTLV
                     TRVIFEGRRAIGVEAVEEGKVVVHRAEREVILSGGAYNSPQLLMLSGVGPAAHLRAVG
                     VRVLEDLPVGENLQDHPGTILTWTTDQPVSLLTAESAENLDNFTTNGRGPLTSNVAES
                     GGFVRTRPGLPAPDIQFHATPVMFLGEGLVPPPEHGHSIGPTLLKPESRGSVTLASAD
                     PAAKALIRANYFESENDMASMVAGVRLAMEIAAAPPLKDYRKGAYEVPASTSEEDVRS
                     FIRRYCQTIYHPTGTCAMGAVVDPELRVRGLEGLRVVDASVMPTVVRGNTNAPTIMIG
                     EKASDLIKKSTRAGATATRAAAQPAR"
     gene            complement(1791..2831)
                     /locus_tag="VIN32_08180"
     CDS             complement(1791..2831)
                     /locus_tag="VIN32_08180"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEY8922590.1"
                     /translation="MKAVTMHGLKDVRVEDVPRPEIQEPSDVILRMTTAAICGSDLHL
                     YHDKMPGLLPGSVVGHEFLGVVEEVGSAVRLVEAGKRYVASFYPACGACAACMRHDWR
                     NCPFFAVFGFGEAFGGLHGSQAEYVRVPLADMTLAPVPDSLTDEQALFTGDILSTAFT
                     GVLKSGLQAGQTLAVVGAGPVGLLVVQCAQLVGPSKIFSIDLVPERLKLAQSFGAIPI
                     NAAEGDASDAVFEQTGFLGVDVVIEAVGNAKSLETAWSVARTGATIVIVGVLVDEPWP
                     ISCGDNWLRALTVKPILGDCLTYRYDILKLIEAGKLRPEAIISHTMRLDDATEAYEMF
                     DSRQATKILLKP"
     gene            complement(2881..3288)
                     /locus_tag="VIN32_08185"
     CDS             complement(2881..3288)
                     /locus_tag="VIN32_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016889191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HEY8922591.1"
                     /translation="MAYFKDREEVYQYIGAIFEQALSDPVLGPKFAESGVVLKLVYTD
                     PDSTLVVDMPGGKVYRDQADLKPTVEMFMKADVAHRFWLGKVNIGMALAKGDMRAKGP
                     VPKILKLVPLASSLYPRYRQMLVDDGRQDLAGA"
     gene            complement(3323..>3533)
                     /locus_tag="VIN32_08190"
     CDS             complement(3323..>3533)
                     /locus_tag="VIN32_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012686931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY8922592.1"
                     /translation="AAGIWTRDLARAHRTAAALQAGTVWINAYNVLDPSTPFGGYKES
                     GFGRDLGEEALLHYTQTKCVWVQLD"
BASE COUNT          558 a         1202 c         1191 g          582 t
ORIGIN      
        1 ccagtacgtc gccgagggac ggaccttgaa cgaggccgcc caggagatct tcctgagccc
       61 gtacacgatc cgcgactacc tgctgagcgc gatgcacaag ctgggcgccc agaaccgcgc
      121 ggaagccgtg gtcagagcca ccaagctcgg cctcgtcgtc tgacgccgtt cagcgtgcgg
      181 gttgggcggc cgccctcgtt gccgtggccc ctgccctggt cgatttcttt atcaggtcgg
      241 aagccttctc cccgatcatg atggtggggg cgttggtgtt gccccgcacc acggtcggca
      301 tcaccgaggc atccacgacg cgaaggccct ccaggcctcg gacgcgcagc tcgggatcga
      361 ccacagcgcc catggcgcag gtccccgtcg ggtggtagat ggtctggcaa tagcggcgga
      421 tgaaagacct gacgtcttcc tccgacgtcg aggccggcac ctcgtaggcg cccttccggt
      481 agtccttgag aggcggcgcg gcggcgatct ccatcgccag ccggaccccg gcgaccatgc
      541 tggccatgtc gttctcgctc tcgaagtagt tggcacgaat cagcgccttg gcggccggat
      601 cggccgaggc cagcgtcacg ctgccgcggc tttcaggctt gagcagggtg ggcccgatcg
      661 aatggccgtg ctcgggcggc gggaccaagc cttcgcccag gaacatcacc ggggtggcgt
      721 ggaactggat gtcgggcgcc ggcaggccgg gccgggtgcg cacgaagccg ccggactccg
      781 cgacgttgga ggtgagcggc cccctgccgt tggtggtgaa gttgtcgagg ttctcggctg
      841 actccgcggt cagcaggctc acaggctggt ccgtggtcca ggtgaggatc gtgcccgggt
      901 ggtcctgcag gttctcgccg acgggcagat cctccagcac cctcaccccg actgcgcgca
      961 gatgagccgc cggccccaca cccgacagca tcaggagctg cggcgagttg taggcgccgc
     1021 cgctgaggat gacctcgcgc tcggcgcgat ggaccacgac cttgccttct tccaccgcct
     1081 cgactccgat cgcccggcgc ccttcgaaga tgacgcgggt gaccagcgtg ccggtccgca
     1141 cggccaggtt gggccgcgac atggcggggt gcagataggc cacggcggcg ctgcagcggc
     1201 ggccattgcg ctgggtcacc tggtagtagc cggcgccgtc ctgctcctga ccgttgaagt
     1261 cctcgttgcg aggcagcccg gccgccaccg cggactcgac gaaagcgcgc gccagcgggt
     1321 tctgcgaacg gccttcggac accgacagcg gtccgcccgc gccgtgatac ttggaggcgc
     1381 cccgctcgtt gtcctcggcc ctcttgaagt agggcaggac gtcctcatac ccccagccgg
     1441 ggttgcccag gtcgcgccac tcgtcgtagt cggcccggtt gccccggatg tagaccatgg
     1501 cgttgatcga cgagcttcca cccagcaccc ggcctcgagg gtggtagatg cggcggccat
     1561 ccagatgcgc ctccggttcc gtgctgtagt cccagtcgaa gggcgtcttg aagagccgcg
     1621 agaacgcgac cgggacgtgg atcccgggct cggagtccgg cgggcccgcc tcgagcagga
     1681 gaaccttgac ctcggcgtcc tctgtaagcc tggcggcgag gacgcagccg gccgagccgg
     1741 ccccgacgac gatgtagtcg tatgtgctct tcatcgcatc ccctatctgc tcagggcttg
     1801 agcaggatct tggtggcctg ccgggagtcg aacatctcat aggcctcggt ggcgtcgtcg
     1861 agccgcatgg tgtgggagat gatcgcctcc ggccgcagct tgccggcctc gatcagcttc
     1921 aggatgtcgt agcggtaggt gaggcaatcc ccgaggatcg gcttgacggt gagcgcgcgc
     1981 agccagttgt cgccgcagct gatcggccag ggttcgtcca ccagcacgcc gacgatgacg
     2041 atggtggcac cggtcctggc caccgaccag gccgtctcca ggctcttggc gttgcccacc
     2101 gcctcgatca ccacgtcgac gcccagaaag cccgtctgct cgaagaccgc gtccgaggcg
     2161 tcaccctcgg cagcgttgat ggggatggcg ccgaacgact gggcgagctt cagccgctcg
     2221 ggcacgaggt cgatggaaaa gatcttggag ggccccacga gctgggcgca ctgcacgacc
     2281 aacaggccga ccggcccggc gccgaccacg gccagcgtct gtccggcttg aaggccggac
     2341 ttcagcaccc cggtgaatgc ggtcgagagg atgtcccccg tgaacagcgc ctgctcgtcg
     2401 gtgagcgagt cggggacggg agccagggtc atgtcggcga gcggcacccg tacgtactcg
     2461 gcctgcgagc catgcaggcc accgaacgcc tcgccaaagc cgaagacggc gaagaagggg
     2521 cagttgcgcc agtcgtgccg catgcacgcg gcgcaggcac cgcaggcagg atagaaggag
     2581 gccacgtagc gcttgcccgc ctccaccagg cggaccgccg agccgacctc ctcgacgacc
     2641 ccgaggaact cgtggccgac caccgagccg ggcagcagcc cgggcatctt gtcgtggtag
     2701 agatgcaggt cgctgccgca gattgccgcc gtcgtcatcc tcaagatgac atcggatggc
     2761 tcctgtatct ccgggcgcgg gacatcctcc accctcacat ctttcagtcc gtgcatggtc
     2821 acagccttca tggccggctc ctccctcctc gtcgatccat accgatgcgg gcgcgccgca
     2881 ctacgcgccg gccaggtcct gccggccgtc gtcgaccagc atctggcggt agcgggggta
     2941 gagggagctg gcgaggggga ccagcttcag gatcttgggt accggtccct tggcccgcat
     3001 gtcacccttg gcgagggcca tcccgatgtt caccttgccg agccagaacc ggtgggcgac
     3061 atcggccttc atgaacatct cgacggtcgg cttgaggtct gcctgatcgc ggtagacctt
     3121 gccgccgggc atgtccacca ccagggtaga gtcgggatcg gtgtagacga gcttgagcac
     3181 cacccccgac tcagcgaact ttgggcccag caccggatcg ctgagcgcct gctcgaagat
     3241 cgcaccgatg tattggtaga cctcttcgcg gtccttgaaa taggccatca ctcacacctc
     3301 ccgattcttg cctgcaccac gatcagtcga gctggaccca gacgcacttg gtctgggtgt
     3361 aatggaggag cgcctcctcg ccgagatctc gcccgaagcc ggactccttg tagcccccga
     3421 acggtgtgct cgggtcgagg acgttgtacg cgttgatcca gaccgtgccc gcctgcaggg
     3481 ccgctgcggt gcggtgggcc cgggccaggt cgcgggtcca gatcccagcc gcc
//
LOCUS       DATCQR010000247         5279 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_230361, whole genome shotgun sequence.
ACCESSION   DATCQR010000247 DATCQR010000000
VERSION     DATCQR010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5279
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230361"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /locus_tag="VIN32_08195"
     CDS             complement(<1..462)
                     /locus_tag="VIN32_08195"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY8922593.1"
                     /translation="MVQTGDRRRVTVPDGDGPTRRAGHRRLPGVDLRPGAVKQARMEA
                     GLSLAKVGQGCVTAPAVYLIETGRTRPSLPTLEHIARRLGKSVEFFLADQSGGADDTR
                     TGLIELEAMVAEGRHQEAITHGEELLKLSTSAHRLGQLRFLLGQAYLTLAQS"
     gene            complement(1165..2436)
                     /locus_tag="VIN32_08200"
     CDS             complement(1165..2436)
                     /locus_tag="VIN32_08200"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: protein motif:HMM:NF002086.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEY8922594.1"
                     /translation="MNSVRPNLDAAYAHTTERVMILDTTLRDGEGSPGFHLDANSKVR
                     IARQLEKLGVDVIEAGFPISSRGDFEACRRIAREIRGTGVTALARAVREDIDAVWAAI
                     KEAETPQIHIVLSVSDVHIKRKLGMTRQQVLKQGAEMVAYARTLCDRVEYSPEDAGRA
                     DPEYLLETVETMIAAGATVINIPDTTGYCLPGEFGELVHRLIADARGADKVLFSVHCH
                     DDLGLATANALAALTAGARRVECTVNGIGERAGNTSLEEIVMLLRVRQSRLGLECAVE
                     PTEITRTSRLVAELTGTPVQPNKAVVGANAFAHAAGIHQDGILKDRLNYEIMTPEDVG
                     LADSRIVLTARSGRHAFHHRLSRLGVVLTEAQTQAAWKLFKEVADNKREVTDEDLRKI
                     VTAPESGGGGFADQSNDHNHVADTLRHLIFG"
     gene            complement(2433..2909)
                     /gene="ilvN"
                     /locus_tag="VIN32_08205"
     CDS             complement(2433..2909)
                     /gene="ilvN"
                     /locus_tag="VIN32_08205"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEY8922595.1"
                     /translation="MIEEATRVRVLSVLVEDGSSTLTKVTGMIRRRGFHVRAITVGPS
                     GEPGRSRMTLQIDAGHAEVDQVRKQLERLVEVLEIEDLTEHAVHSRELVVARVAAAAV
                     PELVAKGARVLQPGSVLTTIEFVGDGNEVATFVEDLRRHGILDVVRSGPVVMRRTA"
     gene            complement(2906..4588)
                     /gene="ilvB"
                     /locus_tag="VIN32_08210"
     CDS             complement(2906..4588)
                     /gene="ilvB"
                     /locus_tag="VIN32_08210"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012191973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HEY8922596.1"
                     /translation="MTGADLVLEALEQEGVDLIFGYPGGANLPIYQRLPEHPKLKHIL
                     VRHEQGAAHMADGYARACGRPGVVFATSGPGALNLVTGLCTAYMDSVPMIAITGQVNS
                     TQVGRDAFQESDVIGATSSVTKHSYLVTDIAQLPRVIHEAFHIATTGRPGPVLIDICK
                     DVQMAEAERTPRAPLSIPGYHLNGHVKLDDAKIAALALAHAERPVILAGHGVLLSHAE
                     QALLDVAEKAGVPVGTTLLGIGGFPVNHPLSLHMTGFMGTGWCLKAVQNADVLMMVGM
                     RVDDRVTARLADFAPRVTTFIHVDIDGSEMGKNVRPHIELVADARKALEAMLPHIDSV
                     DEGRGAWLTQIDAWREEHPLRYTHSNGHLQPQDVLIDMYRRCRSEAVVVADVGQNQIW
                     AALWWNYDRPGLFINSGGAGTMGFALPAAIGAKFARPDLDVWCVAGEGGFVMTAQELS
                     VAVEHQLDIKIVLLNNFSLGMVRQFQDDFYGGVRSQVELTHIPDFVKLADAYGLPGVR
                     VEKLADVGPAMDAAERTKGPFLIDFRIDPEANVYPIVPLGKSLNDFWEAPQQ"
     gene            4883..>5279
                     /locus_tag="VIN32_08215"
     CDS             4883..>5279
                     /locus_tag="VIN32_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013151242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HEY8922597.1"
                     /translation="MPTTLAKKLWDSHVVRSTPEAPDLLYVDLHLVHEVTSPQAFEGL
                     RLAGRKVRRPDLTVATMDHNVPTRGGVRAADELSRKQMEVLAENCRREGIPLHQIGSR
                     RQGIVHVIGPELGLTQPGMLIVCGDSHTST"
BASE COUNT          823 a         1757 c         1794 g          905 t
ORIGIN      
        1 cgactgggcc agcgtcaaat acgcttggcc gagcaggaag cgcagctggc ccaggcggtg
       61 cgccgacgtg ctgagcttta gcaactcttc gccatgcgtg atcgcttcct ggtgccggcc
      121 ttcggccacc atcgcctcga gctcgatgag cccggtcctg gtgtcatcgg cgccaccgct
      181 ctggtcggcc aggaagaact cgaccgactt gccgagccgg cgggcaatgt gctcaagagt
      241 gggcagcgag gggcgcgtgc ggcccgtttc gatcaggtag accgcagggg ctgtaacgca
      301 gccctggcca accttggcga ggctgaggcc ggcctccatg cgggcctgct tgaccgcccc
      361 gggccggaga tctactccag gcaaacgccg atgcccggca cgccgagtcg ggccgtcccc
      421 gtcgggaacg gtcactcgcc ggcggtcacc ggtctgaacc acgctgggag cttaaccgaa
      481 attacttaac ggtggcacgg gcgtgtggca tccggcgcgg caggcgcgcc tgtggcagca
      541 atcccggcag ggcttactta ggcttgggcc tacttaagcg gtggccatac atcggccaac
      601 ccggtcgtcg agaagagggc catcagagcg gcggccatcg tggcgacggt gaagacgaat
      661 ccgaacctgc gcatatctct cacctcccag gccgcgtaaa tggacgcgcg cccgcataaa
      721 ggctagagtt aacggggccg ttaagctagt ttcagatcga gttggacggt cgatccatcg
      781 ccttccggcg cgcaggtccg cggtagagtg cttccccgcc gcggcctgcg ccccggtcgg
      841 cggtctgttt tccaggacaa ggcgtggcgt ccaggccgga tttactccgg ccatcaccga
      901 gcctcggcag ggaccgccgc tacattcact ccttctcgcg catctggcta cgaaaaggcg
      961 cagcgtccgc tggggccccc gcgactctgt cgcgggagct tgttcctcac ccgcgccgtt
     1021 acccgacaag gcgtggcgtc aaggccggat ttactccggc cgtcacccag ccctccattt
     1081 ccaaacacct ccaggcgtcg tggctaacag gagggggttc cgcgggggag gacttgtgtc
     1141 ctcacccgcg ccgttatcgc gccgttatcc gaagatcagg tgccggagag tgtccgcgac
     1201 gtggttgtga tcgttggact ggtcggcaaa accgcccccg ccgctctccg gggccgtgac
     1261 gatcttgcgg aggtcctcgt cggtgacctc gcgcttgttg tcggcgacct ctttgaacag
     1321 cttccaggca gcctgcgtct gggcctccgt cagcaccacg ccgagccgac tcagccggtg
     1381 gtggaaggcg tggcgccccg accgggcggt cagcacgatc cgcgaatctg caaggccgac
     1441 gtcctccggc gtcatgatct cgtagttcaa gcggtccttc aggatgccgt cctggtggat
     1501 gcccgcggcg tgcgcgaacg cgttggcgcc cacgaccgcc ttgttcggct gcaccggcgt
     1561 gccggtgagc tccgcgacca gccgtgacgt ccgggtgatc tcggtgggtt ccacggcgca
     1621 ttcgaggccg agcctcgact gccggacgcg aaggagcatg acgatctcct cgagggatgt
     1681 gttgccggcc cgctcgccga tgccattgac ggtgcactcc acgcgacgcg ccccggcggt
     1741 gagggcggcc agcgcgttgg ccgtcgccag gccaaggtcg tcgtggcagt gcacggagaa
     1801 caggaccttg tcggcgcctc gcgcgtccgc gatgaggcgg tgcaccagct ccccgaactc
     1861 tccgggcagg cagtagccgg tggtgtccgg gatgttgatg acggtcgcgc cggcggcgat
     1921 catcgtctcg accgtctcca ggaggtactc ggggtcggcg cggcccgcgt cctcgggcga
     1981 atactcgacc cggtcgcaca gcgtgcgcgc gtaggcgacc atctcggcgc cctgcttgag
     2041 gacctgctgg cgcgtcatgc cgagcttgcg cttgatgtgg acgtcggaga cggaaagaac
     2101 gatgtggatc tgcggagtct cggcctcttt gatcgcggcc cacaccgcgt cgatgtcttc
     2161 gcgcacggcg cgcgccagcg cggtcacgcc ggtgccacgg atctcccgcg cgatgcggcg
     2221 gcaggcttca aagtcgccgc gcgacgagat ggggaagccc gcctcgatga cgtcgacgcc
     2281 aagcttctcg agctggcgcg cgatgcggac cttggagttg gcgtcgaggt ggaagccagg
     2341 cgacccctcg ccgtcgcgca gcgtggtgtc gagaatcatt acgcgttccg tggtgtgcgc
     2401 atacgcggca tccaggttgg gccttactga attcatgccg tcctcctcat gacgacgggg
     2461 cccgagcgga ccacgtcgag aattccgtga cgtctcaaat cttcgacaaa cgttgcgacc
     2521 tcgttgccat cgccgacgaa ctcgatggtc gtcagcaccg acccgggctg cagcaccctg
     2581 gcgccctttg caacgagctc cgggacggcc gctgccgcca cgcgggccac gaccagctcg
     2641 cggctgtgca cggcgtgctc ggtgaggtct tcgatctcaa gcacctcgac gaggcgctcc
     2701 agctgcttgc ggacctggtc cacctcggca tggccggcgt cgatctggag cgtcatgcgg
     2761 gagcggccgg gctcgccgct gggtccgacc gtgatcgcgc gcacgtggaa gccgcgccgg
     2821 cggatcatgc ctgtgacctt ggtcagggtg ctcgagccgt cctcgaccaa taccgacagg
     2881 acgcgtacac gggtcgcctc ttcgatcact gctgcggagc ctcccagaag tcgttcaggc
     2941 tcttgccgag cggaacgatc gggtagacgt tggcctcggg gtcgatgcgg aagtcgatca
     3001 ggaacgggcc cttggtgcgc tcggccgcat ccatcgccgg ccccacgtcg gcgagcttct
     3061 cgacccgcac ccctggcagc ccgtaggcgt ccgcgagctt gacgaagtcg ggaatgtggg
     3121 tgagctccac ctgggagcgc acgccgccgt agaagtcgtc ctggaactgg cggaccatcc
     3181 caagcgagaa gttgttgagg agaacgatct tgatgtcgag ctggtgctcg acggcaacgg
     3241 acagctcttg cgccgtcatc acgaagccgc cctcgccagc gacgcaccac acgtccaggt
     3301 cgggccttgc gaacttggcc ccgatggccg ccggcagcgc gaagcccatg gtgccggcgc
     3361 cgcctgaatt gatgaacaac cccggtcggt cgtagttcca ccagagagcc gcccagatct
     3421 ggttctgccc cacgtcggcg acgaccaccg cctcgctgcg gcagcgcctg tacatgtcga
     3481 tgagcacgtc ctgcggctgc aggtggccgt tcgagtgggt gtagcggagc gggtgctcct
     3541 ctcgccacgc gtcgatctgc gtgagccagg cgccgcggcc ctcatccacc gaatcgatgt
     3601 gcgggagcat cgcctcgagg gccttgcgcg cgtctgcgac cagctcgatg tgcgggcgaa
     3661 cgttcttgcc catctcggag ccgtcgatgt cgacgtggat gaacgtcgtg accctgggcg
     3721 cgaagtcggc gaggcgggcg gtgacccggt cgtcgacccg catgccgacc atcatcagga
     3781 cgtcggcgtt ctggaccgcc ttgaggcacc agccggtgcc catgaacccg gtcatatgca
     3841 gcgacagagg atggttgacg gggaacccgc cgatgcccag aagcgtcgta cccacgggga
     3901 ctccggcctt ctcggcgacg tcgagcagcg cctgctctgc gtggctgagg aggacgccgt
     3961 ggccggcgag gatgacgggc cgttccgcat gcgcaagggc aagcgctgcg atcttggcgt
     4021 cgtcgagctt gacgtggccg ttcaggtggt agccgggaat gctgaggggc gctcgcggcg
     4081 tccgctcggc ctcggccatc tggacgtcct tgcagatgtc gatgagcact ggccccggcc
     4141 ggccggtggt ggcgatgtgg aaggcctcgt ggatcacccg cggcagctgc gcgatgtcgg
     4201 tgacgaggta gctgtgcttg gtcaccgagc tggtggcccc gatgacgtcg gactcctgga
     4261 aggcatccct gccgacctgg gtgctgttga cctggccggt gatggcgatc atcggcacgg
     4321 agtccatgta ggcggtgcag aggccggtga cgaggttcag ggcgccggga cccgaggtgg
     4381 cgaagacgac gcccggacgg ccgcaggcgc gggcgtagcc gtccgccatg tgcgcggcgc
     4441 cctgctcgtg gcgcaccagg atgtgcttga gcttggggtg ctcgggcagg cgctgataga
     4501 tcggcaggtt cgccccgccg gggtagccga agatgaggtc gacgccctcc tgctccagcg
     4561 cctccagcac caggtccgct ccggtcactt cgtctccttc aaaacatggc cttacaacaa
     4621 gaaagccccc gcctttcggt cgggggcttt gcgatttgac tctttctgac taggtcgcgc
     4681 gcgcccccgt ttcgccgagg ccaataaggc ccaggatccc caggagtacc agtacgcggc
     4741 cgttggcaac catttctgtc attgcatggt aacgaagctg gatggcgttc tgttgtgtcc
     4801 tgcacacaaa ttcaggcgcg atacctttga accggcgcca cgggcaggag cggttaaggg
     4861 ccggtaaagt tggccttccg ccatgccgac cacgctcgcc aagaagctct gggattcgca
     4921 cgtcgtccga tccacgcccg aggcacccga ccttctctac gtcgacctcc acctcgtcca
     4981 cgaggtgacc tcgccccagg cgtttgaggg cctgcggctg gcaggacgca aggtccgccg
     5041 gcccgacctg actgtggcga cgatggacca caacgtcccc acccggggcg gcgtccgcgc
     5101 cgccgacgag ctgtcgcgca agcagatgga ggtgctggcg gagaactgcc ggcgcgaggg
     5161 catcccgctc caccagatcg gcagccgccg ccagggcatc gtgcacgtga tcggcccgga
     5221 gctgggcctg acccagcccg gaatgctcat cgtgtgtggc gactcgcaca cctcgactc
//
LOCUS       DATCQR010000248         4576 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_60517, whole genome shotgun sequence.
ACCESSION   DATCQR010000248 DATCQR010000000
VERSION     DATCQR010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4576
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60517"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..301)
                     /locus_tag="VIN32_08220"
     CDS             complement(<1..301)
                     /locus_tag="VIN32_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HEY8922598.1"
                     /translation="MLAETLSASIMGVEGLPVRVEVDVAFGLPALTIVGLAGSQVQEA
                     RERVRSALRNSGFEVPARRITINLSPADLPKDGTGYDLPIAVGILAASGQLRDVER"
     gene            complement(446..1633)
                     /locus_tag="VIN32_08225"
     CDS             complement(446..1633)
                     /locus_tag="VIN32_08225"
                     /inference="COORDINATES: protein motif:HMM:NF025001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8922599.1"
                     /translation="MATRVLVVDDDLNIQRVLVFALKQEGYAVTVASDGAAGVEMALD
                     ARPDLILMDAAMPKLDGYAATQQIRAAEKGVGRIPIIMLTSEADVGQRVRGLRAGADD
                     DIVKPFHPLELMARIKALLAQSAPVSAKTGGDAPTIGKLCAFYPAKGGVGTTTIAINT
                     GIALARELKRRTALIDANLQFGDLRVFLDLGLDTPSIVSAVSEPDLDLDLYKTMMVSH
                     RSGIDLLLAPPTPASADIVVERQRQAPTVLSNILSQSRRLYDYTLVDMSQAIDDFNLQ
                     LFDEADVIYVVMTADLSCLKNVPLVLETMDSLGYEKSKVQLVLNRSNAYTGINVSNAE
                     SALGRKIDYQVINEYRGAISALNSGEPFMSSRADAPLSKSVLAFARAIDKAFAASAVP
                     ARA"
     gene            complement(1668..2594)
                     /locus_tag="VIN32_08230"
     CDS             complement(1668..2594)
                     /locus_tag="VIN32_08230"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY8922600.1"
                     /translation="MATPPRRGRGLIGWLARIAAAVTFGYLGYLTVEGSRRIVSPGRR
                     PFVPEEGDPATPADIGLEYEDVRFTTDDGVTLSGWLVPAARETRAAVVLMHGFSWHRL
                     PWLAGFVPWLQRRYHVLQFDFRGHGGSDDALISLGTLEQRDVAAAVHFLAGRGLGPIA
                     LMGISMGGSVAIMAAPDLPVAAVVADAAYADLRDPIANRMREIGYPLARLGTGLVLAA
                     ASLRARVWLRSPMNRVSQIAPRGLLLISPGEDRLVSPQQSQRMFRAAGEPKELFVVEG
                     AAHGEAHLTAPEAYERRVLDFLARHLDGEAPV"
     gene            complement(2612..3508)
                     /locus_tag="VIN32_08235"
     CDS             complement(2612..3508)
                     /locus_tag="VIN32_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018035117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEY8922601.1"
                     /translation="MPDNLVGLVILLVGLAVLLVLVTSVVHIIREYERLVVFFLGRLQ
                     GARGPGLVLLIPFVQQAVKVDLRERFLEVPQQTCITKDNAPISIDFLVYSKVFDPVET
                     VTAVVDFTGASQALAATTLRAVIGDIPLDDVLAKREEINNVLRSKLDEVTHRWGVKIT
                     NVEIREIVPPRDIQEAMNRQMSAERNRRANITESEGTRQATINVAEGDKQSAILRAEG
                     ERKAQVLRAEGFSNALKTIFGAAKGVDEKTMTLQYLEALKALAAGASTKWIVPMELAE
                     LTRPLAGFMRAAREAAEPTPKT"
     gene            complement(3541..>4576)
                     /locus_tag="VIN32_08240"
     CDS             complement(3541..>4576)
                     /locus_tag="VIN32_08240"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEY8922602.1"
                     /translation="ASPILSPFASFPAILGHEVVGMVEEAGSAVEVRTGERVVVDPII
                     SCFVRGLEPCSACRDGLPALCRHAADGSLSPGMLIGYCRDLPGAWSEAMLAHASQLHR
                     VPDSMSDETAVLVEPLACSLHAVLAAPPGRDEKVLVVGGGTIGLGVLVALRMVDPGAD
                     VTAVVRHPFQASLAERLGASRVVLDRGGDGPQRAAVEVTGARVHRPIVGDEVLTGGFD
                     LVFDGVGSRDSVDACLRVVAPRGRLVLLGTAGELEHLDLTLAWARELRIVGSYVYGRE
                     ASLPGSPHTFDYLLQRLVEPGAPAVAELVTHRFGLDQWRQALAVATGRGRHASVKVVF
                     DHATAYAASD"
BASE COUNT          717 a         1617 c         1558 g          684 t
ORIGIN      
        1 gccgttcgac gtcgcgcagc tggcccgagg cggccaggat ccccaccgcg atcggaaggt
       61 catagcccgt cccgtccttt ggcaggtctg ccggcgagag gttgatggtg atgcgccggg
      121 ccgggacttc gaagccgctg ttgcgcaggg cgctccgcac ccgctcgcgg gcctcctgca
      181 cctggctccc cgccaggccc acgatggtga gcgccggcag accgaaggcg acgtccacct
      241 cgacgcggac cggcagcccc tcgacgccca tgatcgaagc gctgagcgtc tctgccagca
      301 cgcgcaccac ctcgcctcgg ggaggcggat cggagcgaat gaggtgccgg ccgcacacct
      361 gctcgcctcc caaactccgg cggcgaccgg ctgagcagga ggctagggat cgagccttat
      421 cggcccgtta ggcgaggctt atcggtcagg cgcgagccgg aacggcgctg gccgcgaagg
      481 ccttgtcgat ggcccgtgcg aaggccagca cgctcttgct caagggggca tcggcccggc
      541 tggacatgaa cggctcgccc gagttgaggg cgctgatcgc gccgcggtac tcgttgatga
      601 cctggtagtc gatcttgcgg ccgagcgccg actccgcgtt gctcacgttg atgccggtgt
      661 acgcattgga ccgattcagg acgagctgca ccttgctctt ctcgtagccc aggctgtcca
      721 tcgtctcgag caccagcggt acgttcttaa ggcacgaaag gtccgcggtc atgaccacgt
      781 agatgacatc ggcctcgtcg aagagctgga ggttgaagtc gtcgatcgcc tgcgacatgt
      841 cgaccagcgt gtagtcgtac aggcgtcgcg actggctcaa gatgttgctg agcacggtgg
      901 gagcctgtcg ctggcgctcg accacgatgt cggcgctcgc cggtgtcggc ggagccagca
      961 gcaggtcgat cccggaccga tggctgacca tcatggtctt gtacaggtcc aggtccaggt
     1021 ccggctcgct gaccgcgctc acgatcgagg gagtgtcgag gcccaggtcc aggaacacgc
     1081 gcaggtcgcc gaactggagg ttggcgtcga tcagggctgt ccgtcgcttg agctcacgcg
     1141 ctagcgcgat gccggtgttg atggcgatcg tcgtcgtccc gacgccgccc ttggcgggat
     1201 agaacgcgca cagcttgccg atcgtcggcg cgtcgccgcc cgtcttggcg ctgacaggag
     1261 cgctctgcgc cagcagcgcc ttgatgcgcg ccatcagctc gagcggatgg aacggcttga
     1321 cgatgtcgtc gtcggcgccg gcgcggaggc ccctgacccg ctgtccaacg tcagcctcgg
     1381 aggtgagcat gatgatcggg atgcggccga cccccttctc ggccgcccgg atctgctgcg
     1441 tggctgcgta gccgtccagc ttgggcatgg ccgcgtccat caggatcagg tccggcctgg
     1501 cgtcgagtgc catctccacg ccggcggcgc cgtctgaggc gaccgtgacg gcgtacccct
     1561 cctgcttgag ggcaaagacc aggacgcgct ggatgttgag gtcgtcgtcg acgaccagga
     1621 ccctcgtggc catgcagctc cccgtggagg attggaggtc cggccgatta tacgggcgct
     1681 tccccatcca ggtggcgggc caggaagtcc aggacgcggc gctcgtaggc ctcgggggcg
     1741 gtgaggtgcg cctcgccatg ggccgctccc tcgaccacga acagctcttt cggctcgccc
     1801 gcggcgcgga acatgcgctg cgattgctgg gggctcacca gccgatcctc ccccggcgag
     1861 atgagcagca gcccacgcgg cgcgatctgc gaaacccggt tcatcggcga gcgcagccag
     1921 acccgcgctc gcaggctcgc ggccgcgagc accagcccgg tgcccagccg agccaaggga
     1981 tacccgatct cgcgcatccg gttggcgatc gggtcgcgca ggtctgcgta ggcggcatcg
     2041 gctacgacgg cagccaccgg gaggtcgggc gcggccatga tcgccactga gccgcccatg
     2101 ctgatcccca tcagcgcgat cggccccaac ccacgaccgg ccaggaagtg caccgccgcg
     2161 gccacgtcgc gctgctcgag cgtcccgagc gagatgagcg cgtcgtcgct ccccccatgg
     2221 ccgcggaagt cgaactggag gacgtggtag cgccgctgga gccacggcac gaagccggcc
     2281 agccacggca ggcggtgcca cgagaagccg tgcatcagga ccaccgcggc acgcgtctcg
     2341 cgcgcggcgg ggacgagcca gccggacagg gtcacgccat catcggtcgt gaagcggacg
     2401 tcctcgtatt ccaggccgat gtccgcgggc gtcgcaggat caccctcctc cggcacgaat
     2461 ggccggcggc cggggctgac gatgcggcga gacccctcga ccgtcaggta gcccagatag
     2521 ccgaaggtca ccgccgcggc gatccgggcc agccagccga tcagcccgcg ccctcggcgc
     2581 ggaggggttg ccaccggact cgaacggtcc ctcaggtctt gggagtcggc tcggcggcct
     2641 cgcgcgcagc gcgcatgaat ccggccagcg gccgcgtcag ctcggcgagc tccatcggaa
     2701 cgatccactt ggtggatgca cccgcggcca gcgccttgag cgcctcgagg tactggaggg
     2761 tcatcgtctt ctcgtcgacc ccctttgccg ccccgaagat cgtcttgagc gcattgctga
     2821 agccttcggc gcgcagtacc tgggcctttc gctcgccctc ggcgcgcagg atggcgctct
     2881 gcttgtcgcc ctcggccacg ttgatggtcg cctgccgggt cccctccgac tcggtgatgt
     2941 tggcgcgccg gttgcgctcg gcgctcatct ggcggttcat cgcctcctgg atgtcgcgcg
     3001 gcggcacgat ctcgcggatc tcgacgttgg tgatcttcac gccccagcgg tgcgtcacct
     3061 cgtcgagctt cgagcgcagg acgttgttga tctcctcgcg cttggccagc acgtcatcga
     3121 gcgggatgtc gccgatcacg gcgcgcagcg tggtcgccgc caatgcctgc gaggcaccgg
     3181 tgaagtccac taccgcggtc acggtctcga cggggtcgaa gaccttggag tagacgagga
     3241 agtcgatgct gatcggcgcg ttgtccttgg tgatgcaggt ctgctgcggc acctccagga
     3301 agcgctcgcg caggtcgacc ttgaccgcct gctggacgaa cgggatgagc agtaccagcc
     3361 ccgggccgcg cgcaccctgc aaccgcccca ggaagaagac gaccagccgc tcgtactcgc
     3421 ggatgatgtg gacgacgctg gtcaccagga cgaggagaac cgccaggccg accagcagga
     3481 tgaccaggcc aaccagattg tcaggcatcc gggagcttcc cttctcgaac ggcgcaggct
     3541 ttagtcgctg gcagcatagg ccgttgcatg atcgaagacc accttcaccg atgcgtgtcg
     3601 cccgcgcccc gtcgcgaccg ccagtgcctg ccgccactgg tcgagcccga accgatgcgt
     3661 gaccagctcc gcgacggctg gcgcgccagg ttcgaccagc cgctgcagca ggtagtcgaa
     3721 cgtgtgcggc gaccccggca gggaggcttc gcgcccgtag acgtagctgc cgacgatccg
     3781 cagctcgcgc gcccacgcga gcgtcagatc gaggtgctcc agctctcccg cagtgccgag
     3841 caggacgagg cgaccccgcg gcgcgacgac ccgcaggcag gcgtccacgc tgtcccggct
     3901 gccgaccccg tcgaagacca ggtcgaagcc gccggtgagg acctcgtccc ccacgatcgg
     3961 ccgatgcact cgcgcgccgg tcacctccac ggcagcccgc tgcggcccgt ccccgccgcg
     4021 atcgagcacg acccggctgg cgcccagccg ctcggccagg ctggcctgga acggatgccg
     4081 caccacggcc gtgacatcag cgccggggtc caccatgcgc agggcaacca gcaccccaag
     4141 cccgatcgtc ccgccgccaa ccaccagcac cttctcgtca cgccccggcg gagcggccag
     4201 gaccgcatga agggagcagg caagcggctc gaccaggacc gcggtctcgt cgctcatcga
     4261 gtccggaacc cggtgcagct ggctcgcgtg cgccagcatc gcctccgacc aggcgccggg
     4321 gaggtcacgg cagtatccga tcagcatccc gggtgacaga gatccgtcgg cggcgtgacg
     4381 gcacagggcc ggcagcccgt cgcggcaggc ggaacacggt tccaggccgc gaacgaagca
     4441 cgagatgatc gggtcgacca ccacccgttc ccccgttctc acctcgacgg cgctgccggc
     4501 ctcctcgacc atgcccacca cctcgtggcc caggatcgcc gggaaggagg caaacggcga
     4561 caggatcggg ctggcc
//
LOCUS       DATCQR010000249         7727 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_45588, whole genome shotgun sequence.
ACCESSION   DATCQR010000249 DATCQR010000000
VERSION     DATCQR010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 7727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7727
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45588"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1200)
                     /locus_tag="VIN32_08245"
     CDS             complement(<1..1200)
                     /locus_tag="VIN32_08245"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY8922603.1"
                     /translation="MRIGIVGTRLAGVDGVTFEAAKWETVLLGLGHEVRLCAGELDAL
                     RSNARLIPAMHFAYPPAARVTAAAFDPASDPASVLGEIERLAGQLLPPLRDWVAANRL
                     EALIIENAWAIPMHLPLGVALRRLVEESGIPTIGHHHDYWWERDRFAGSVVPEILESA
                     FPPDLPSVRHASINSLAAAQLRKRRGLESVVVPNVFDFEQPLPPHRRRVRNRMRRELG
                     MGDSGLLVVQPTRVVPRKGIELAIELVGRLGDRDAVLLITSPAGDEGLEYLVQLELQA
                     ARAGVRLQYAADRFEPDHEGIPIGPAHTLGDAYAAADLITYPSLYEGFGNALIEAIFH
                     GKPVLVNRYPVYVADIAPLGMELIEIDGAITDQTVAAVRAVLANPARQRRVARRNFEI
                     AQRHLSYN"
     gene            1230..2672
                     /gene="der"
                     /locus_tag="VIN32_08250"
     CDS             1230..2672
                     /gene="der"
                     /locus_tag="VIN32_08250"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015254751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEY8922604.1"
                     /translation="MSLPVVAIVGRPNVGKSTLFNRLVGERVAIVEDVPGTTRDRVYG
                     TGEWNGRRFTVVDTGGLELEPGTHIEERVQDQARVAIEESDLVLFVVDAAVGIAPLDH
                     EVADRLRRAGKPTILVVNKADNPRREHEGVEFYALGFEPTVAVSAQHGRSTGDLLDLV
                     VDALPPAEPGEATPEASVYDDRDLVEGDLAELAETEMGPPRVAIVGRPNTGKSTFVNR
                     VLGEERMIVSDLPGTTRDPVDSAVMVDGEPMILVDTAGIRRRGSVDRGIERYSVLRAM
                     KAIERADVAVVMIDAIEGYTAQDAHVVGHVLEAGKGIVLVLNKWDALEKDGHTADDWT
                     KTLRREAPYLAWADIVFASAQTGQRVERILHEARRVAEERFRRIPTGELNRLVTDAVR
                     AHPPAHVRNRQPKILYATQVAIGPPTFVVFVNDPELIHFSYRRYLENRIRAEYGFLGT
                     PIKLIFRQRVSEDAARRAARPRRVARSGRR"
     gene            2675..3889
                     /locus_tag="VIN32_08255"
     CDS             2675..3889
                     /locus_tag="VIN32_08255"
                     /inference="COORDINATES: protein motif:HMM:NF014433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015446 - ATPase-coupled arsenite
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0071722 - detoxification of
                     arsenic-containing substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRC40/GET3/ArsA family transport-energizing
                     ATPase"
                     /protein_id="HEY8922605.1"
                     /translation="MKPRILLYTGKGGVGKTSVAAATALACAKRGYRTMVLSTDIAHS
                     LGDVFGVELGPQPKEIAPNMWGQESDVYFNVDRYWGRIQEYAASVLRWRGLDEVIAEE
                     MTVLPGMDEVGSLLWIAEHHDSGDYDVIVVDAAPTGETLRLLSLPEAGKWWMEKLEPI
                     GRRITKLTGPFIQRVVGMPMPGEDVFNAGEELFRRLEHMHDLLIDPELTSVRVVLTLE
                     QVVIKEAQRSFTYFHLYGYPTDLVVANRVLPDAVGWKYFRGWYERQQRYGPMVEQAFA
                     PIPILSAPFLDQEVIGLPVLTELGEQLYGDADPTKQFYVGRPYSVERQDGELVLSVEL
                     PFATKEQISLSRHADELVIDVGTWRRNLVLPRLLVEATTLGAKFDDHILRIRFAAPPR
                     STNGGKKRGRDG"
     gene            3882..4148
                     /locus_tag="VIN32_08260"
     CDS             3882..4148
                     /locus_tag="VIN32_08260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8922606.1"
                     /translation="MDSASKADLEARIADLERRLPRGERREGVETAFWAVMRNIFPSE
                     TRTHVKAAGREQLLAARSYLDHWIAKMDAEPAEEKGTRESIEVE"
     gene            4145..5167
                     /locus_tag="VIN32_08265"
     CDS             4145..5167
                     /locus_tag="VIN32_08265"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014742403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEY8922607.1"
                     /translation="MTDQVGVVGAGAWGTTLAIKLAAAERPVTLWAHTAEAADELASL
                     RENRRYLPGIVFPPNLRVATDDAYLSEPHRSFVLAVPSAHLRETLRRLRSTLFAEASL
                     LSVVKGIEQGTHLRMSEVIEEELPSRRVAALSGPNLAREIAEGLPAVSVVASPDVELG
                     GEFAALLGSESFRLYTNPDLVGVELSGALKNVVALAAGFVDGLGFGDSAKAGIITRGL
                     AEMTRLGVAAGGQQLTFAGLAGVGDLIATCMSPLSRNRRAGELMASGMPWADAAAVLN
                     GVAEGVDTVRGALELAVELGVEMPIAEQVAAVVHQGRPPMEAVAELMSRVQKDELTGL
                     WAQPAR"
     gene            5177..6127
                     /gene="speB"
                     /locus_tag="VIN32_08270"
     CDS             5177..6127
                     /gene="speB"
                     /locus_tag="VIN32_08270"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HEY8922608.1"
                     /translation="MPPRYVPEDSLQSPRFTGPSTFARLPHVRTLEEVDVAIVGVPFD
                     TGVTYRVGGRFGPNAVRAASVMLRPYNANLDVKPFEILSCVDYGDVAIIPGNTERSYQ
                     AIEETVAPIVQAGVVPLLLGGDHACTLPHLRATRTRGPVAVIDFDAHTDAWDSYFGEK
                     YNHGTWMRRAIEEGLVDTAHSIEVGLRGSIYDAEDRTGLRTELGLDYLTTEDVFELGP
                     AAVASRIRERVGDQPAFISFDIDVVDPGFAPGTGTPEAGGPSAHDMLAVLRGLTGIDF
                     IGFDVVEVIPAYDPAGQTATLAANLAYEMLSLVALRRAGS"
     gene            6133..7059
                     /locus_tag="VIN32_08275"
     CDS             6133..7059
                     /locus_tag="VIN32_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012688732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HEY8922609.1"
                     /translation="MPEAPGLDWHSPMPLGFAWPEGKRAAACFTFDVDAESPILFDHP
                     ESAGWLDVMSHQAYGPRTGVPRLLRVLDRQGIRATFFVPGYTAERWPEAIRAIRDAGH
                     EIGHHGYLHEGAHSAPDAETEERHLLRGLEALRDVIGVRPVGYRAPMWELSYRLPALL
                     AKHGFLYDSGLMDADHPYRLATGPESGASSIIELPAHWSLDDWEPYAYLPGITGSGVI
                     ASPTEVLERWTLELEALVAEGGLFMLTNHPFVSGRASRAVALETLMERARAIDGLWIA
                     AADQIAAHVATLPLEPVVHRPHAPVASRGPDG"
     gene            complement(7085..>7727)
                     /locus_tag="VIN32_08280"
     CDS             complement(7085..>7727)
                     /locus_tag="VIN32_08280"
                     /inference="COORDINATES: protein motif:HMM:NF014218.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HEY8922610.1"
                     /translation="LEILGVLVATNPAAAGGESSDTIFALLGGSGALMAGFAMVAIFI
                     GTIAVNAMNDYTGSLSLQAAGFKVKRIYSAAIVAILGFGFTLVLQYNGEFSGNFFNFL
                     LFISYWISPFVGVVLADWWLRRGQADPYSVVDYAKLHTGTIGLVALVVGFLVGIPFEA
                     SSLGYAWSAEWWGVPFNWVTANYLHGADLAYYVGGGVAFLIYWFGARRSLQRT"
BASE COUNT         1230 a         2586 c         2717 g         1194 t
ORIGIN      
        1 gttgtaggag aggtgccgct gcgcgatctc gaagttgcga cgggcaacac gacgctgacg
       61 agcggggttg gcgagcacgg cgcgcaccgc cgcgaccgtc tggtcggtga tggcgccgtc
      121 gatctcgatc agctccatgc caagcggcgc gatgtcggcc acgtagaccg ggtatcggtt
      181 cacgagcacc ggcttgccgt ggaagatggc ctcgatcagg gcgttgccga acccctcgta
      241 caggctcggg taggtgatca ggtcggccgc cgcatacgcg tcgcccaggg tgtgggctgg
      301 tccgatcggg atcccctcgt ggtcgggctc gaaccgatcc gcggcgtact gcagccggac
      361 cccggcgcgg gcggcctgga gctcgagctg gacgaggtac tcgagtccct cgtcgccggc
      421 cgggctggtg atgaggagca ccgcgtcccg gtcgccgagc cggccgacca gctcgatggc
      481 gagctcgatc cccttgcgtg gcacgacccg cgtgggctgc accaccagca gtcccgaatc
      541 gcccattccg agctcgcgcc gcatccggtt gcgcacccgg cggcggtggg gcggcagcgg
      601 ctgctcgaag tcgaacacgt tggggacgac aaccgactcg agcccgcgcc gctttcgaag
      661 ctgcgcagcg gcaaggctgt tgatgctcgc gtggcggacg ctgggcaggt cgggaggaaa
      721 ggcgctctcc aggatctcgg ggacgaccga ccccgcgaac cgatctcgtt cccaccagta
      781 gtcgtggtgg tgaccgatgg tcgggatccc ggactcctcc accaggcgtc gcagggcgac
      841 cccgagggga aggtgcatcg ggatcgccca ggcgttctcg atgatgaggg cctccagccg
      901 attggccgcg acccagtcgc gcaggggcgg cagcagctgg ccggcgagcc gctcgatctc
      961 gcccaggacc gaggcaggat cgctggcggg gtcgaatgcc gccgccgtga cgcgcgccgc
     1021 cggcgggtag gcgaagtgca tggcggggat gagtcgagcg ttggagcgca gggcatccag
     1081 ctcgcccgcg cagagccgga cctcgtgtcc gaggccgagg agcaccgtct cccacttggc
     1141 ggcctcgaag gtgaccccat caaccccggc cagccgggtt ccaacgatcc cgatccgcat
     1201 cccggccatg ctacccgtac actcgggcca tgtcccttcc cgtggtcgcc atcgtcggcc
     1261 gccccaatgt cggcaagtcg acactcttca accggctggt cggggagcgg gtggcgatcg
     1321 tcgaggacgt ccccgggacc acccgtgacc gggtgtatgg caccggcgag tggaacgggc
     1381 gccgcttcac cgtggtggac acgggcgggc tggagctaga gccggggacc cacatcgagg
     1441 agcgcgtgca ggaccaggcg cgcgttgcga tcgaggagtc cgacctcgtc ctcttcgtgg
     1501 tggacgctgc cgtgggcatc gcgccgctcg accacgaggt cgcggatcgc ctgaggcgcg
     1561 cgggcaagcc gacgatcctg gtcgtcaaca aggccgacaa ccctcggcgt gagcacgagg
     1621 gggtggagtt ctacgcgctc ggattcgagc cgaccgtggc cgtcagcgcg cagcacggcc
     1681 gcagcacggg cgacctcctc gacctggtgg tggacgcgct tccgccggca gagcccggcg
     1741 aagcgacgcc ggaggcatcg gtctatgacg accgcgacct ggtcgaggga gacctggccg
     1801 aactggccga gaccgagatg gggccgcctc gagtcgcgat cgtcggccgt cccaacaccg
     1861 gcaagtccac cttcgtcaat cgcgtgctgg gcgaggagcg gatgatcgtc tcggacctgc
     1921 ccgggacgac acgcgacccg gtcgactcgg cggtgatggt cgacggtgag ccgatgatcc
     1981 tggtggacac ggccggcatc cggcgccgcg gctcagtcga tcgcggtatc gagcgctact
     2041 cggtcctgcg tgccatgaag gcgatcgagc gggcggacgt ggcggtggtc atgatcgacg
     2101 ccatcgaggg atacaccgcc caggatgccc acgtggtcgg ccacgtgctg gaggcgggca
     2161 aggggatcgt gctggtcctg aacaagtggg acgccctgga gaaggacggc cacaccgccg
     2221 atgactggac gaagaccctg cgccgcgagg cgccctacct ggcctgggcc gacatcgtgt
     2281 tcgcctcggc ccagaccggc cagcgggtcg agcgcatcct gcacgaggcg cgtcgcgtgg
     2341 ccgaggagcg cttccgtcgc attccgaccg gggagctcaa ccgactggtg accgatgcgg
     2401 ttcgcgccca cccgcccgcg cacgtgcgca acaggcaacc gaagatcctg tacgccaccc
     2461 aggtggcgat cggcccgccc accttcgtcg tcttcgtcaa cgatccggag ctgatccact
     2521 tcagctaccg gcgctacctc gagaaccgga tccgggccga gtacggcttc ctcggcaccc
     2581 cgatcaagct gatcttcaga cagcgcgtct ccgaggatgc cgcgcgccgc gcggcccgcc
     2641 cgcgacgggt cgctcgctcc ggccggcgct gagcgtgaag ccgcgcatcc tcctgtacac
     2701 cggcaagggc ggcgtcggca agacgagcgt cgccgctgcc acggcgctcg cctgcgccaa
     2761 gcgcggctac cgcaccatgg tcctctcgac cgacatcgcg cacagcctgg gcgacgtctt
     2821 cggggtcgag ctggggccgc agccgaagga gatcgcgccg aacatgtggg gccaggagtc
     2881 cgacgtctac ttcaacgtgg atcggtactg gggtcgaatc caggagtacg ccgccagtgt
     2941 gctgcgctgg cgcggcctgg acgaggtgat cgccgaggag atgaccgtgc tgccgggcat
     3001 ggacgaggtc ggtagcctgc tgtggatcgc cgagcaccac gactctggcg actacgacgt
     3061 gatcgtggtc gacgcggcgc cgaccggtga gacgctgcgg ctcctttcgc tgcccgaagc
     3121 cggcaagtgg tggatggaga agctcgagcc gatcggccga cgcatcacga agctgaccgg
     3181 accgttcatc cagcgcgtgg tcgggatgcc gatgcccgga gaggacgtct tcaatgccgg
     3241 cgaggagctc tttcgtcggc tcgagcacat gcatgacctc ctgatcgacc ccgagctgac
     3301 ctcggtccgt gtggtgctga cgctggagca ggtggtcatc aaggaagcgc agcgctcgtt
     3361 cacctacttc catctgtacg gctacccgac cgatctggtc gtcgccaacc gcgtgctgcc
     3421 tgatgccgtg gggtggaaat acttccgggg ctggtacgag cggcagcagc gctacggccc
     3481 catggtcgag caggcgtttg cgccgatccc gatcctcagc gcgccattcc tcgaccagga
     3541 ggtgatcggg cttccggttc tcaccgagct cggcgagcag ctctatggcg acgctgaccc
     3601 gacgaagcag ttctacgttg ggcgcccgta ctcggtggag cgccaggacg gtgagctcgt
     3661 tctatccgtg gagctgccct tcgcgacaaa ggagcagatc tccctctccc ggcacgccga
     3721 cgagctggtc atcgacgtcg gcacctggcg gcgtaatctg gtcctgccgc gcctcctcgt
     3781 ggaggcgacg acactgggcg ccaagttcga cgaccatatt ctcaggattc gcttcgctgc
     3841 cccgccgcga agcacgaatg gaggtaaaaa gcgtggcaga gatggatagc gcctcgaagg
     3901 ccgacctcga ggctcgtatc gccgacctgg agcgccgctt gccgcgaggc gagcgacgtg
     3961 agggcgtgga gaccgccttc tgggcggtca tgcgcaacat cttccccagt gagacgcgca
     4021 cccatgtgaa ggcggccggg cgtgagcagc tgctcgcagc tcgaagctac ctggatcact
     4081 ggatcgccaa gatggacgcg gagcctgccg aggagaaggg gacgcgcgag agcatcgagg
     4141 tcgaatgacc gatcaggtgg gggtggtggg ggccggcgcg tggggcacca cgctcgccat
     4201 caagcttgcc gccgcggagc gtcctgtcac cctctgggcg cacaccgcgg aggctgcgga
     4261 cgagctcgcc tcactccgcg agaaccgccg ctacctgccc gggatcgtct tcccgcccaa
     4321 cctgcgggtg gcgaccgatg atgcctacct gtcggagccc catcgctcgt tcgtcctcgc
     4381 cgttccctcg gcgcacctgc gggagacgct gcgacgtctg cggtccacgc tcttcgccga
     4441 ggcgtccctg ttgagcgtcg tgaaggggat cgagcaggga acccatctgc gcatgagcga
     4501 ggtgatcgag gaggagctgc cgagccgccg agtggcggca ctctccgggc cgaacctggc
     4561 gcgcgagatc gcggagggcc tgccggcggt gagcgtggtg gcctcgcctg acgtcgagct
     4621 ggggggggag ttcgccgcgc tgctgggcag cgagagcttc cgcctctaca ccaacccgga
     4681 cctggtcggc gtcgagctgt ccggcgccct caagaacgtg gttgccctcg ccgctggatt
     4741 cgtcgatggg ctcggcttcg gcgacagcgc caaggcgggc atcatcaccc gtgggctggc
     4801 cgaaatgacc cggctgggcg tcgctgccgg aggccagcag ctgaccttcg ccgggctggc
     4861 tggggtcggc gacctgatcg cgacctgcat gagcccgctc tcccgcaatc gacgcgccgg
     4921 cgagctgatg gcgtccggca tgccctgggc ggatgccgct gcggtcctga atggcgtggc
     4981 cgagggagtg gacacggtgc ggggcgcgct cgagctggcc gtcgaactgg gcgtggagat
     5041 gccgattgcg gagcaggtcg ccgccgtcgt gcaccagggg cgtccgccga tggaagcggt
     5101 tgccgagctg atgtcccggg tgcagaagga cgagctgacc ggcctgtggg cgcagcccgc
     5161 gcgctagact catcccgtgc ctccgcggta tgtccccgag gactctctcc aaagtccccg
     5221 cttcaccggt ccctcaacct tcgcgcggct gccgcatgtc cgaacgctgg aggaggtgga
     5281 cgtcgcgatc gtgggcgtcc cgttcgacac cggggtgacc taccgggtcg gcggccgatt
     5341 cggcccgaat gcggtccggg cggcgagcgt gatgctgcgg ccgtacaacg ccaacctgga
     5401 tgtcaagccc ttcgagatcc tctcgtgcgt cgactatggc gacgtggcca tcattccggg
     5461 caacaccgag cggagctacc aggccatcga ggagacggtt gccccgatcg tccaagcggg
     5521 agtcgtgcca ctcctactcg gcggcgacca cgcgtgcacc ctcccgcacc tgcgggcgac
     5581 gcgaaccagg ggcccggtgg cggtcatcga cttcgatgcg cacaccgatg cctgggacag
     5641 ctacttcggc gagaagtaca accacgggac ctggatgcgg cgagccatcg aggagggtct
     5701 ggtggatacc gcccattcga tcgaggtcgg cctgcgcggg tcgatctacg acgccgagga
     5761 tcggaccggc ctgcggacgg agctgggact ggattacctg accaccgagg atgtcttcga
     5821 gcttggtcct gcggctgtcg cgtcccgcat ccgggagcga gtcggcgatc agccggcctt
     5881 catcagcttc gacatcgatg tcgtggatcc tggattcgcc ccgggcaccg gaacgcccga
     5941 ggcgggtggg ccatcggcgc acgacatgct ggccgtcctg cgcgggctga ctgggatcga
     6001 cttcatcggc ttcgacgtgg tcgaggtgat tccggcctac gatcctgccg gccagaccgc
     6061 gacgctggcg gcgaatctcg cctacgagat gctctcattg gtcgcgctgc ggcgcgctgg
     6121 cagctgaggc cgatgccgga ggctcccggg ctcgactggc acagcccgat gccgcttggc
     6181 tttgcgtggc cggagggcaa gcgagcggcc gcctgcttca ccttcgacgt cgacgcggag
     6241 agcccgatcc tgttcgacca ccccgagagt gcgggttggc tcgacgtcat gtcgcaccag
     6301 gcctatgggc cacgaaccgg ggttccgcgg ctgctgcgcg tcctggaccg gcaggggatc
     6361 cgcgccacct tcttcgtgcc cggctacacc gccgagcgct ggcctgaagc gatccgcgcc
     6421 atccgcgatg cggggcatga gatcggtcat cacgggtacc tgcacgaagg ggcgcattcg
     6481 gcccccgatg ccgagacgga ggagcggcat ctgctccgtg ggctggaggc cctgcgggac
     6541 gtcatcggcg tgcggccggt ggggtatcga gcgccgatgt gggagctctc ctaccgcctc
     6601 cccgcccttc tggcgaagca cgggttcctg tacgacagcg gcctcatgga cgcggaccat
     6661 ccctaccggc tcgcgacagg tccggagtcg ggagcgtctt cgatcattga gctgccggcg
     6721 cactggagcc ttgacgactg ggagccatat gcctacctgc cggggatcac cggctccggc
     6781 gtgatcgcca gcccgaccga ggtgctcgag cgctggacgc tggagctgga ggcgctcgtc
     6841 gctgaagggg gcctcttcat gctcaccaac caccccttcg tctcagggcg ggcatcgcgg
     6901 gccgttgccc tcgagacgct gatggagcgg gcacgtgcga tcgacggact gtggatcgcc
     6961 gcggccgatc agatcgctgc gcacgtggcg accctgccgc tcgagccagt cgtccatcgg
     7021 ccgcacgccc cggttgcatc aagaggcccc gacggatgac cgccggggcc tcccgtgggt
     7081 cagctcaggt tcgttgcagc gagcgtcgcg cgccgaacca atagatcagg aaagccactc
     7141 cgccgcccac gtagtacgcc aggtcggcgc catggaggta gttggcggtg acccagttga
     7201 agggcacgcc ccaccattcg gcgctccacg cgtaccccag ggacgaggcc tcgaagggga
     7261 tcccgaccag gaagccgacg accagcgcca ccagcccgat cgtcccggtg tgcaactttg
     7321 catagtcgac caccgaatac ggatcggcct gccccctccg cagccaccaa tccgccagga
     7381 cgaccccgac gaagggtgag atccagtagc tgatgaagag caggaagttg aagaagttcc
     7441 ctgagaactc tccgttgtac tggagcacca gggtgaaccc gaagccgagg attgccacga
     7501 tggccgcaga gtagatgcgc ttcaccttga atccagccgc ctggagcgac agagagccgg
     7561 tgtagtcgtt catcgcgttg acggcgatcg tcccgatgaa gatggcgacc atcgcgaagc
     7621 cggccatcaa ggcgccgctc ccgcctagca aggcgaagat cgtgtcgctg gactcgccgc
     7681 cggcagccgc cgggttggtg gcgaccagca cgccgaggat ctcgagc
//
LOCUS       DATCQR010000250         2792 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_215676, whole genome shotgun sequence.
ACCESSION   DATCQR010000250 DATCQR010000000
VERSION     DATCQR010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2792
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215676"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1059)
                     /gene="uvrC"
                     /locus_tag="VIN32_08285"
     CDS             complement(<1..1059)
                     /gene="uvrC"
                     /locus_tag="VIN32_08285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEY8922611.1"
                     /translation="MTLDGQREAHLRTVLRQLPLSPGVYLMKGPDGRVLYVGKADLLR
                     HRVRSYFGSKTGLDRRILRMTAEVADIEYIVTDTVSEAFLLESNLIKEHRPRFNIRLR
                     DDKSYPFVKITLGEDFPRIVRTRKLVRDGSRYFGPYASASSVDETLKLLRRIFPFRTC
                     NLDIPDGKRVLERPCLLYYIKRCQGPCIQAIEKAEYRATIDRVVDFLEGRQEGIAAEL
                     RGEMQDHSHALRYEQAAIARDKLRAVERTIEQQKVAAFSRAEQDVIGIAREEGEACLQ
                     LFVIRNGKMIGREHFIVEGARDVTDAEVLGSFLPQYYAATERPPREVVLPFAPSEADA
                     LAAFLADRRGSKVALTVPE"
     gene            complement(1056..2237)
                     /locus_tag="VIN32_08290"
     CDS             complement(1056..2237)
                     /locus_tag="VIN32_08290"
                     /inference="COORDINATES: protein motif:HMM:NF015755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEY8922612.1"
                     /translation="MNRRDRRPNHATESPRARRIREATEREQLLRARGIDPLSPPPRR
                     SRASTRRPRRWKRNLVLLLVILLLSGCTGGWLIWQRVAAFNDDVSSAGSLSSSLLFPL
                     IGSDRVNVAIYGYGGPGHTGGIYLADSIMILSIDPKTDTTTMIPIPRDLWIQGFPELP
                     NNAKINEAFADGYVRGGVYEAGKFATSVLAEVTGLPIQHWMALDFAGFRAMVDSVGGV
                     TVDNPVAFAYTWDENRFFAHKWDGGSFESGPIFLDGVHALDYARSRYTSVTAESTDFA
                     RSVRQQRVLIAIRGKLGGGLAALGPGLAIMDALKGQMATDLSAIDLYLLSGHLGVDRR
                     IELTEGVILEATSNSIGQYILVVIGRASGSDYAPLRQYLQTELARPLPTPLPSPSPSQ
                     P"
     gene            2350..>2792
                     /locus_tag="VIN32_08295"
     CDS             2350..>2792
                     /locus_tag="VIN32_08295"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEY8922613.1"
                     /translation="MALAQAVIPTVAPLLDALDAEQQAAATLPDGPAQIIAPAGSGKT
                     TTLIARLGVLLTRGVAAERIAVVTFNREAAEELSARIALRLGPLLPEADGIEVRTLHA
                     LARQVLLDAGVGVHLVADRLPLLRAARRRVIARRSPDAPPVPEAAA"
BASE COUNT          429 a          921 c          998 g          444 t
ORIGIN      
        1 ctcgggcacg gtcagcgcca ccttcgagcc acgccggtcg gcgaggaagg cggccagcgc
       61 atcggcctcc gatggtgcga acggcagcac cacctcgcgc ggcggccgct cggtcgccgc
      121 gtagtactgc ggcaggaagg agccgagcac ctcggcgtcg gtcacgtcgc gcgccccctc
      181 cacgatgaag tgctcgcggc cgatcatctt cccgttgcgg atcacgaaca gctggaggca
      241 ggcctcgccc tcctcgcgag cgatcccgat cacgtcctgc tcggcccggc tgaaggcggc
      301 gaccttctgc tgctcgatgg tgcgctcgac ggcgcgcagc ttgtcgcggg cgatggcggc
      361 ctgctcgtac cgaagggcat gcgagtggtc ctgcatctcg ccgcgcagct cggccgcgat
      421 cccctcctgg cgtccctcca ggaagtcgac cacgcgatcg atggtggcgc ggtactcggc
      481 cttctcgatg gcctggatgc acggcccctg gcagcgcttg atgtagtaca ggaggcacgg
      541 gcgttcgagg acccgctttc cgtcggggat gtccaggttg caggtccgga acgggaagat
      601 gcggcgcagc agcttgaggg tctcgtcgac gctggaggcg gaggcgtacg ggccgaagta
      661 gcggctgccg tcccggacca gcttgcgggt gcgcacgatc cgcggaaagt cttcgccgag
      721 cgtgatcttc acgaacgggt agctcttgtc gtcgcggagg cggatgttga agcggggccg
      781 atgctccttg atcaggttgg actcgagcag gaacgcctcg ctcaccgtgt cggtgacgat
      841 gtactcgatg tccgcgacct cggccgtcat gcgcaggatc cgcctgtcga ggcctgtctt
      901 cgagccgaaa taggaacgga cccgatgccg aagcaggtcc gccttgccga cgtacaggac
      961 ccggccgtcg ggccccttca tcaggtacac gcccggtgac aggggcagct ggcgcagcac
     1021 ggtgcgcagg tgcgcctccc gctggccgtc gagggtcatg gctggctcgg cgaagggctc
     1081 ggaagggggg tgggaagcgg ccgcgccagc tcagtctgca ggtattgccg caacggggcg
     1141 tagtcgctcc ccgaagcccg cccgatgacg acgaggatgt actggccgat gctgttgctg
     1201 gtcgcctcca ggatcacgcc ctcggtcagc tcgatgcgcc gatccacgcc gaggtggccg
     1261 gagagcaggt acaggtcgat cgccgacagg tcggtggcca tctggccctt cagggcatcc
     1321 atgatcgcaa gccccggtcc cagggcagcc agtcccccgc cgagcttgcc acggatcgcg
     1381 atcagcacgc gctgctggcg aacggatcgc gcgaagtccg tcgattcggc agtcacactg
     1441 gtgtaccgcg aacgcgcgta gtccagggcg tgcacgccgt cgaggaagat cggtcccgac
     1501 tcgaaggagc cgccgtccca cttgtgcgcg aagaacctgt tctcgtccca ggtgtaggcg
     1561 aacgcgaccg ggttgtccac cgtcacaccc ccgacgctgt cgaccatcgc cctgaacccg
     1621 gcgaagtcga gcgccatcca gtgctggatg gggaggccgg tcacctcggc gaggaccgag
     1681 gtggcgaatt tgcccgcctc gtacacgcca ccgcgcacgt agccgtccgc gaacgcctcg
     1741 ttgatcttgg cgttgttcgg aagctcgggg aagccctgga tccacaggtc gcgggggatc
     1801 gggatcatgg tggtcgtatc ggtctttgga tcgatcgaga ggatcatgat cgagtcggcc
     1861 aggtagatcc ctccggtgtg ccccggcccc ccgtagccgt agatggcaac gttgacgcgg
     1921 tcgctgccga tgagcgggaa gaggagggag gatgagaggg agccggccga gctgacgtcg
     1981 tcgttgaagg ccgccacgcg ctgccagatg agccatccgc cggtgcatcc gctcagcagc
     2041 agaatgacca gcagcaggac caggttgcgc ttccagcgcc gtgggcggcg cgtcgacgcc
     2101 cgtgatcggc gcggaggagg actcagcggg tcgatgccgc gcgcgcgcag cagctgctcg
     2161 cgttcggtgg cctcccggat ccggcgggcg cgcggacttt cggtggcgtg gttgggccgg
     2221 cggtcgcgcc ggttcatcgg caactcatgg gttcggtgaa gggtcccctt atgtaagcga
     2281 gcggacgaca ggatatcccg ataagcgggc gatcatgcac tggtaaggct gcgggccgat
     2341 cctgtcctca tggcgctggc ccaggccgtg atcccaaccg tggctcccct gctcgatgcg
     2401 ctcgatgcgg agcagcaagc cgccgcaacc ctgccggatg ggccagccca gatcatcgct
     2461 cccgcgggca gtggcaagac cacgacactc atcgcccggc tcggggttct gctgacgcgc
     2521 ggcgtggccg ccgagcggat cgcggtggtc accttcaacc gcgaggctgc ggaggagctg
     2581 agcgcgcgga tcgccctgcg gctgggtccg ctactgcccg aagcggacgg gatcgaggtc
     2641 cggacgctgc acgcactggc gcgtcaggtc ctcctcgacg caggcgttgg agtgcacttg
     2701 gtcgcggacc gcctcccgct gctgcgcgcg gcgcgacggc gcgtgatcgc aaggcgatct
     2761 ccggacgccc cgccggtgcc ggaggcggcc gc
//
LOCUS       DATCQR010000251         2370 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_230423, whole genome shotgun sequence.
ACCESSION   DATCQR010000251 DATCQR010000000
VERSION     DATCQR010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2370
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230423"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /locus_tag="VIN32_08300"
     CDS             complement(<1..462)
                     /locus_tag="VIN32_08300"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY8922614.1"
                     /translation="MVQTGDRRRVTVPDGDGPTRRAGHRRLPGVDLRPGAVKQARVEA
                     GLSLAKVGKGCVTAPAVYLIETGRTRPSLPTLEHIARRLGKSVEFFLADQSGNADETR
                     TGLIELEAMVAEGRHQEAITHGEELLKLSTSAHRLGQLRFLLGQAYLTLAQS"
     gene            complement(1018..2250)
                     /locus_tag="VIN32_08305"
     CDS             complement(1018..2250)
                     /locus_tag="VIN32_08305"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: protein motif:HMM:NF002086.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEY8922615.1"
                     /translation="MTPEHVMILDTTLRDGEGSPGFHLDANSKVRIARQLEKLGVDVI
                     EAGFPISSRGDFEACRRIAREIRGTGVTALARAVREDIDAVWAAIKEAETPQIHIVLS
                     VSDVHIKRKLGMTRQQVLKQGAEMVAYARTLCDRVEYSPEDAGRADPEYLLETVETMI
                     AAGATVINIPDTTGYCLPGEFGELVHRLIADARGADKVLFSVHCHDDLGLATANALAA
                     LTAGARRVECTVNGIGERAGNTSLEEIVMLLRVRQSRLGLECAVEPTEITRTSRLVAE
                     LTGTPVQPNKAVVGANAFAHAAGIHQDGILKDRLNYEIMKPEDVGLSDSRIVLTARSG
                     RHAFHHRLSRLGVVLTEAQTQAAWKLFKEVADNKREVTDEDLRKIVTAPESGGGGFAD
                     QSNDHNHVADTLRHLIFG"
BASE COUNT          365 a          790 c          799 g          416 t
ORIGIN      
        1 cgactgggcc agcgtcaaat acgcttggcc gagcaggaag cgcagctggc ccaggcggtg
       61 cgccgacgtg ctgagcttta gcaactcttc gccatgcgtg atcgcttcct ggtgccggcc
      121 ttcggccacc atcgcctcga gttcgatgag cccggtcctg gtctcgtcgg cgttgccgct
      181 ctggtcggcc aggaagaact cgaccgactt gccgaggcgg cgggcgatgt gctcgagagt
      241 gggcagtgag gggcgtgtgc gaccggtctc gatcaagtag acggccgggg ccgtgacgca
      301 gcccttgcca accttggcga ggctgaggcc cgcctcaacg cgggcctgct tgaccgcccc
      361 ggggcggaga tctactccag gcagacgccg atgcccggca cgccgagtgg ggccgtcccc
      421 gtcgggaacg gtcactcgcc ggcggtcacc ggtctgaacc acgctgggag cttaacccaa
      481 attacttaac ggtgccacgg gcgtgtggca tccggcgcga cagcgcgcct gtggcagcaa
      541 tcccggcagg gcttatttag gcttggctac ttaagcggtg gccatacatc ggccaaaccg
      601 gtcgccgcga aaagggccat cagagcggcg gccatagtgg cgatgctgaa tacgaacccg
      661 aacttgcgca tatctctcac ctcccaggcc gcgtaaatgg acgcgcgcct gcataaagcc
      721 tagagttaac gaaggcgtta agctagtttc aggatcaggt tgggcggtcg atcgatcgcc
      781 ttccggcgcg caggcccgcg gtagagtgct tccccgccgc ggcctgcgct ccgggtcggc
      841 ggtaccgttt gcaggacaag gcgtggcgtc caggccggat ttactccggc cgtcacccag
      901 cctcggccgg gaccgccgct acattcactc cttctcgtgc atccgcctac gaaaaggcgc
      961 agcgtccact ggggcccccg cgactctgtc gcgggagctt gctcctcacc cgcgccgtta
     1021 gccgaagatc aggtgccgga gcgtgtccgc aacgtggttg tggtcgttgg actggtcggc
     1081 gaagccgccc ccaccgctct ccggggccgt gacgatcttg cggaggtcct cgtcggtcac
     1141 ctcacgcttg ttgtcagcga cctccttgaa cagcttccag gccgcctgcg tctgggcctc
     1201 cgtcagcacc actccgagcc ggctcagccg gtggtggaag gcgtggcgcc ccgaccgggc
     1261 ggtcagcacg atccgagagt ccgaaaggcc gacgtcctcc ggcttcatga tctcgtagtt
     1321 caggcggtcc ttcaggatgc cgtcctggtg gatgcccgcg gcgtgcgcga acgcgttggc
     1381 gcccacgaca gccttgttcg gctgcacagg cgtgcccgtg agctccgcga ccagccgtga
     1441 cgtccgcgtg atctccgtcg gctcgaccgc gcattccagc ccgagacgcg actggcgaac
     1501 gcgaaggagc atcacgatct cctcgaggga cgtgttgccg gcgcgctcgc cgatgccgtt
     1561 gacggtgcac tcgacgcgac gcgctccggc ggtgagggcg gccagcgcgt tggccgtggc
     1621 cagtcccagg tcgtcgtggc aatgcacaga gaacaggacc ttgtcggcgc cgcgcgcatc
     1681 cgcgatgagg cggtgcacga gctccccgaa ctctccgggc aggcagtagc cggtggtgtc
     1741 cgggatgttg atgacggtcg caccggcggc gatcatcgtc tcgaccgtct ccaggaggta
     1801 ctcggggtcg gcgcggcctg cgtcttccgg cgaatactcg acccggtcgc acagcgtgcg
     1861 cgcgtaggcg accatctcgg cgccctgctt gagcacctgc tggcgcgtca tgccgagctt
     1921 gcgcttgatg tgcacgtcgg agacggaaag gacgatgtgg atctgcgggg tctcggcctc
     1981 tttgatggcg gcccacaccg catcgatatc ttcgcgcacg gcgcgcgcaa gcgcggtcac
     2041 gccggtgccc cggatctccc gggcaatgcg gcggcaggct tcgaagtcgc cgcgcgacga
     2101 gatcgggaag cccgcctcga tgacatcgac gccaagcttc tcgagctggc gcgcgatgcg
     2161 gactttggag ttggcgtcga ggtggaagcc aggcgatccc tcgccgtcgc gcagcgtggt
     2221 gtcgagaatc attacgtgct ccggggtcat gctgtcctcc tcatgacgac ggggcccgag
     2281 cggaccacgt cgagatttcc gtgacgcctg agctcttcaa cgaacgctcc gacctcttcg
     2341 ccttctccgg cgaactcgat ggtcgtcgag
//
LOCUS       DATCQR010000252         2045 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_230577, whole genome shotgun sequence.
ACCESSION   DATCQR010000252 DATCQR010000000
VERSION     DATCQR010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230577"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(76..357)
                     /locus_tag="VIN32_08310"
     CDS             complement(76..357)
                     /locus_tag="VIN32_08310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8922616.1"
                     /translation="MTLAASILLGFPLGLWLMFRRRTQVIFGVVWLAILAFQTWDIAF
                     DPGAPNRGVQTKWYYWVGQPVIFAVGVTLMWVGSRARTRLRRRSAQVDG"
     gene            567..>2045
                     /locus_tag="VIN32_08315"
     CDS             567..>2045
                     /locus_tag="VIN32_08315"
                     /inference="COORDINATES: protein motif:HMM:NF019350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEY8922617.1"
                     /translation="MTTILAWSLCAVSVAVAGAALGLHILSGGVDPKGVTDGWASRVL
                     VVPLPMVMAALIAAGRPRNPLGWLLAGGITFEAIAGLLAEYALYGRTTAPGSLPATSA
                     AIFLSTFIWTPAIAAFVLMVMLFPDGRLPSPAKRWRAVAAAALGSSLALMVSTALAPY
                     PVASARPAGFAGLQNPLSGVGTALSDPIGALGGLLLVASAAGAIWALVLRFRRSRGAE
                     REQIAWVVYAAWIPLALFVAHLLPLQLPIFVGPVAICVFAVAMAIAVLRHRLLDIHVV
                     VSRTVVLVLLVLALGALYVAVVEEASTFMRGAAGAAPSVLATAAVAIALAPLHQLLQR
                     TVNRLLFGDRGNTGAVMARVGQSIEGTDEPLDVLAALSETLVAALKLKGARIELGADP
                     AADRPGEGEGLVTPLLVQGKQVGRVVAVPREGERLGAADHRLLSSLSPHLAIVVRAVQ
                     LGKDLQHSREQLVTAREDERRRIRRDLHDGLGPTLAGVAMQLE"
BASE COUNT          296 a          679 c          710 g          360 t
ORIGIN      
        1 gagtaacgat cgttccgttc ttgctgcccg cggaggccag gaacatggat cgatcgcggt
       61 atcggcgatg ggtcatcagc cgtcgacctg cgcggaccgg cgccggagcc gggtgcgggc
      121 gcgggaacca acccacatga gggtgacgcc gacggcgaag atcaccggct ggccgaccca
      181 gtagtaccac ttggtctgga cgccgcggtt cggggccccg gggtcgaacg cgatgtccca
      241 tgtctggaac gccaggatgg ccagccacac aacgccgaag atgacctggg tgcggcgtcg
      301 gaacattagc cagagaccaa gcggaaaccc gagcaggatc gatgcagcga gtgtcataag
      361 cagtgtctcc aatcagcggc cagccgatgg gtatcggcct gtctcaacca ctgagggcag
      421 tgtctcggtc ccgccgaccc ggcctccaga ggcaggagta atcaatctga cgaggacgcc
      481 gccttagcat gtcccgggac atctccaggt tcgacctggg actgtcagac tccgatcaca
      541 ccatgaataa aactgcggcg tcccagatga ccacgatcct ggcgtggtcc ctctgcgcgg
      601 tatccgtcgc cgtggccggc gctgctctcg ggttacacat cctgagcggc ggcgtggacc
      661 ccaagggcgt gacggatggg tgggccagcc gggtgctggt ggttcccctg cccatggtga
      721 tggcggccct cattgccgcc ggccggccgc gcaacccgct cgggtggctc ctggctggag
      781 gcatcacgtt cgaagccatc gccgggctcc tggccgaata cgcgctctac ggtcggacaa
      841 cggcccctgg ttctctgccc gctacctcgg cggcgatctt cctgagcacc ttcatctgga
      901 caccggcgat cgccgccttt gtcctgatgg tcatgctctt cccggatggc cggcttccat
      961 cgccggccaa gcgctggcgc gccgtcgccg ccgcggcgct gggatcatcg ctggcgctga
     1021 tggtctcgac ggcgctggct ccctacccgg tggcctcggc acggccggct ggtttcgcgg
     1081 gactgcagaa cccgctgtcc ggtgtcggga ctgcgctatc cgacccgatc ggagcgttag
     1141 ggggcctgtt gctcgtggcc tccgcggcgg gcgcgatatg ggccctggtc ctgcgcttcc
     1201 gccgcagccg cggtgccgag cgcgaacaga tcgcatgggt cgtatacgcc gcctggatcc
     1261 cactggcact cttcgttgcc cacctcttgc cgctgcaact gccgatcttc gtcgggccgg
     1321 tggcgatatg cgtcttcgcg gttgcgatgg cgatcgcggt gttgcgacac cggctgctcg
     1381 acatccacgt cgtcgtcagt cgaaccgttg tcctggtcct gctggttctg gccctcggtg
     1441 ccctctacgt cgccgtggtg gaggaggctt cgaccttcat gcgaggcgcc gcgggggccg
     1501 cgccgtcggt gctggcgaca gcggctgtgg cgattgcgct ggcgcccctg caccagctgc
     1561 tgcagcgcac ggtcaatcgc ctcctgtttg gtgaccgcgg gaataccggt gcggtgatgg
     1621 cccgagttgg ccagtcgatc gaagggaccg acgagccgct ggacgtgctc gcggctctct
     1681 cggagacgct ggtcgcggcc ctcaagctga agggtgcgag gatcgagctg ggcgccgatc
     1741 cggcggcgga ccgtcccggc gaaggcgaag gcctggtgac accgcttctg gtccagggca
     1801 agcaggtggg acgcgtggtc gcggttccgc gggagggcga gaggctcggt gcggcggatc
     1861 accggctgct cagctccctc tcccctcatc tcgcgatcgt ggttcgcgcc gtccagctcg
     1921 gcaaggacct gcagcactct cgcgaacaat tggtgaccgc gcgtgaggat gaacgacggc
     1981 gcatccgccg cgacctccac gacgggctgg ggccgacgct ggcaggcgtg gcgatgcagc
     2041 tcgag
//
LOCUS       DATCQR010000253         3819 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_154172, whole genome shotgun sequence.
ACCESSION   DATCQR010000253 DATCQR010000000
VERSION     DATCQR010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3819
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154172"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1617
                     /gene="aspS"
                     /locus_tag="VIN32_08320"
     CDS             <1..1617
                     /gene="aspS"
                     /locus_tag="VIN32_08320"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF001750.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEY8922618.1"
                     /translation="HASAEPARNEWVVQVEGVVRHRPPGTTNGELATGEIEVAVDTFA
                     VLNPSKVPPFYVNEEQPGLDESLRLTYRYLDLRRPPLQGRMLLRAQLASAVRRHLEAA
                     AFVEVETPTLIRSTPEGARDFVVPSRVQPGKFYALPQSPQQLKQLLMVAGYDRYYQLA
                     RAYRDEDLRGDRQLEHTQIDIEMSFVAAEDVMATVEQMVTAVSREALPDRPIMQTPFP
                     RLTYEQAMSRYGSDKPDLRFGMELVDLREPVGDVDFRIFSEALAGGGAVYAIRAPGCG
                     DYSRRQLDELIDLAQRHGAKGLIHVSVQDDGSLHGPLGKFLSLTQTAAILEATGGEAG
                     DLLLIVADTDRLVAAGALGHLRLEMGQRLDLRDATTLAYVWVYPFPMFKWDADLDRWD
                     ATHNPFSAPVWDEEAAMEADPGAVHAQQYDLALNGWELGGGSIRIHRRDLLERAFALM
                     GHSIEGMRDQFGALLDALEYGAPPHGGIAIGLDRWSALLADQDNIREVMAFPKTQSGS
                     DLMMDAPSPITPAQLAELSLRVVDEDPDKP"
     gene            1674..1826
                     /locus_tag="VIN32_08325"
     CDS             1674..1826
                     /locus_tag="VIN32_08325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8922619.1"
                     /translation="MSMRPGAILLIIAVVLFLLAAFKVDLGSLDLVTLGLAAFAGSFL
                     IDGWRR"
     gene            complement(2086..2301)
                     /locus_tag="VIN32_08330"
     CDS             complement(2086..2301)
                     /locus_tag="VIN32_08330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8922620.1"
                     /translation="MTAETIMLVRLLTTAALIGATAEYAAMIRRRVVRRADAREHRVL
                     EDLGLQSLVRVFLTDPPAIEQPARAQP"
     gene            complement(2380..2766)
                     /locus_tag="VIN32_08335"
     CDS             complement(2380..2766)
                     /locus_tag="VIN32_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004672464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ectoine synthase"
                     /protein_id="HEY8922621.1"
                     /translation="MIVRTLEDVVGSDRDVAGEGWRSRRFLLQRDALGFSLHDTTVAA
                     GTELNLQYKHHFEACYCLEGEAEVVDLATGERHAIGPGTMYALDAHDRHTVTVQQDLH
                     LVCVFNPALTGGEVHDADGSFPPPDE"
     gene            2839..>3819
                     /locus_tag="VIN32_08340"
     CDS             2839..>3819
                     /locus_tag="VIN32_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011954963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HEY8922622.1"
                     /translation="MSIYLDHAATTPLRAEVLEAMLPYLTEHYGNASSPHAAGRRARQ
                     GLDEAREAIASILGAKPREIVFTGGGSEADNLAIKGAVWAASARGRHIVTSGVEHKAV
                     LHACAIMERSGFGVTYVPVDRFGRVDPAAVEAAINDHTSLVSIMYANNEVGTIQPIAE
                     IGAICRRRGILFHTDAIQAAGFLPLDVDNLGVDLLSLAAHKVYGPKGVGALFVRQGTA
                     LLPQMQGGAQERQRRAGTENVAGIVGFARALELAQGDASARGVENARLAGLRDRLLAG
                     IRDLEGVEPTGHPTERLPHSASWLIDGVEGGDLVAALDLEGVEGSTGSACT"
BASE COUNT          625 a         1298 c         1292 g          604 t
ORIGIN      
        1 catgcatccg ccgagccggc tcgaaacgaa tgggtggtgc aggtggaggg cgtggtgcgc
       61 catcggcctc cggggacgac caacggcgag cttgcgaccg gcgagatcga ggtcgcggtt
      121 gacacctttg cggtcctcaa ccccagcaag gtgccgccct tctatgtcaa cgaggagcag
      181 cccggtctcg acgagtcact gcgcctgacc tatcgctacc tggacctgcg ccggcctccg
      241 ctccaggggc ggatgctgct gcgggcgcag ctcgcaagtg ccgtgcgccg ccacctcgag
      301 gcggccgcat tcgttgaggt cgagaccccg accctgattc gcagcacgcc cgagggtgcc
      361 cgcgacttcg tggtgcccag ccgcgtccag cccggcaagt tctacgcgtt gccgcagtcc
      421 ccgcagcagc tgaagcagct gctgatggtg gccggctacg accgctacta ccagctggcc
      481 cgcgcctatc gcgacgagga cctgcgaggt gatcggcagc tggagcacac ccagatcgac
      541 atcgagatga gcttcgtggc agccgaggac gtgatggcga ccgtcgagca gatggtgacc
      601 gccgtcagcc gcgaggcgct tccggatcgt ccgatcatgc agaccccgtt cccgcgcctc
      661 acctatgagc aggcgatgag ccgctacggg tcggacaagc ccgacctgcg cttcgggatg
      721 gagctggtcg acctccgcga gccggtcggc gacgtcgact tccggatctt cagcgaagcg
      781 ctcgcggggg gtggcgccgt ctacgccatc cgtgctcctg gctgcggcga ctattcgcgc
      841 cgccagctcg acgagctgat cgatctcgcc cagcgtcacg gtgcgaaggg cctcatccac
      901 gtgtcggtgc aggacgacgg atcgcttcac ggcccgctcg gcaagttcct gtccctgacc
      961 cagacggcag ccatcctcga agcgactggt ggtgaggccg gcgacctgct cctgatcgtg
     1021 gccgacacgg atcgcctggt ggccgccggg gcgctcggcc acctgcggct cgagatgggc
     1081 cagcggctcg acctgcgaga tgccaccacg ctggcgtacg tctgggtcta tcccttcccg
     1141 atgttcaaat gggacgccga cctggaccgc tgggacgcca cccacaaccc gttcagcgct
     1201 ccggtctggg acgaggaggc agccatggag gccgatcccg gcgcggtgca tgcccagcag
     1261 tacgacctcg ccctgaacgg ctgggagctg ggtggcgggt cgatccggat tcatcggcga
     1321 gacctgctgg aacgcgcctt tgcgctgatg gggcacagca tcgagggcat gcgcgaccag
     1381 ttcggcgcac tgctcgacgc gctcgaatat ggcgccccgc cgcacggtgg catcgcgatc
     1441 gggctggacc gatggtctgc cctgctcgcc gaccaggaca acatccggga ggtcatggcc
     1501 ttccccaaga cccagtcggg gagcgacctg atgatggacg cgccctcgcc catcaccccc
     1561 gcacagctgg ccgagctgtc gctgcgggtg gtggatgagg acccagacaa gccctaggcg
     1621 gttgacagtc ggcgtctaat cactgcgggg gccacttgga gaggaggagc caaatgtcca
     1681 tgagaccagg cgccattctg ctgatcatcg cagtcgtcct gtttctactc gctgccttca
     1741 aggtcgacct cggttcgctc gacctcgtaa ccctgggcct ggcggcattc gcgggatcgt
     1801 tcctgatcga cggctggagg cgctagctca gaacagggcc tgcacgcacg acgcgagcag
     1861 ccacgcaagg agcgggctgc tcacggccca gtcggacgaa ttcatacggg ggctgcgtta
     1921 tccgggttga gtgcccggat agccggcata tgcagccctg agcctgcagc ggcgccggtt
     1981 cgagccgcaa caaccgcaat agcgcagccg tgatctgaat ctgaacccga tgagggtcgc
     2041 gccgagcgga ggtcattccg agggctgcgg gtggtcagcc gcgggttacg gctgcgctcg
     2101 tgcgggttgc tcgatggcag gcggatcggt caggaacacg cgcacgaggc tctgcaggcc
     2161 gaggtcctca aggacgcggt gttcgcgagc atcggcccga cgcaccacgc ggcgcctgat
     2221 catggcggcg tactcagccg ttgcgccaat gagcgcggct gtagtgagga gcctgacgag
     2281 catgatcgtc tccgctgtca tgcccaaacc atcggctctg agctgcctgg gcctcaacag
     2341 gacgatggtc atggccctgt tcagctccga ccccgagcct cattcgtctg gaggcgggaa
     2401 gctcccatcg gcgtcgtgaa cttcgccgcc ggtcaacgca ggattgaaga cgcagacgag
     2461 gtggaggtcc tgctgcacgg tgaccgtgtg gcggtcgtga gcgtcgaggg cgtacatggt
     2521 ccccggaccg atcgcatggc gctcgccggt ggccaggtcc accacctccg cctcgccctc
     2581 gaggcagtag caggcctcga agtggtgctt gtactgcagg ttgagctccg ttcccgccgc
     2641 gacggtggtg tcgtgcagcg agaagcccag cgcgtcgcgt tgcaacagga agcgacggct
     2701 gcgccaaccc tctcctgcga cgtcccggtc actccccacc acgtcttcga gagttcgcac
     2761 gatcaccggt gaacacctcc atcatcccca ggtcgtagac tccgccatca tgcacgaggc
     2821 gcccgggaga cgcggccgat gagcatctac ctcgaccatg ccgccaccac gcccctccgc
     2881 gccgaggtgc tggaggcgat gctgccgtac ctgaccgagc actacggcaa cgcgtcgagc
     2941 ccgcatgcag ccggccggcg ggcgcgacag gggctcgacg aggcgcgcga ggcgatcgcc
     3001 tcgatcctcg gcgccaagcc ccgcgagatc gtctttaccg ggggcggcag cgaagccgat
     3061 aacctcgcca tcaagggtgc tgtctgggcc gccagcgcca ggggacggca catcgtcacc
     3121 agcggcgtcg agcacaaggc ggtcctccac gcctgcgcga tcatggagcg ctccggcttc
     3181 ggggtcacct acgtccctgt cgaccgattc gggcgcgtcg atcctgccgc ggtcgaggcc
     3241 gcgatcaacg accacaccag cctggtctcg atcatgtacg ccaacaacga ggtcggcacc
     3301 atccagccga tcgccgagat cggcgccatc tgccgtcggc gcggcattct cttccatacc
     3361 gatgcaatcc aggctgcggg cttccttccg ctcgacgtcg acaacctggg ggtcgacctg
     3421 ctgagcctcg ccgcccacaa ggtctacggc cccaaggggg tcggtgcgct cttcgtgcgg
     3481 caggggacgg cgctcctgcc ccagatgcag ggaggcgcac aggagaggca gcgacgggcc
     3541 ggaaccgaga atgtggccgg catcgtgggc ttcgcgcggg cgcttgagct ggcacagggc
     3601 gacgcctccg ctcgtggcgt tgagaacgcg cggctggcag gcctgcggga tcggcttctc
     3661 gctggcatcc gggacctcga gggagtggag cccaccggcc atccgaccga gaggctgccg
     3721 cacagcgcca gctggctgat cgacggggtc gaaggaggcg atctggtcgc cgcccttgac
     3781 ctcgagggag tcgagggcag cactggcagc gcctgtacc
//
LOCUS       DATCQR010000254         2099 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_199586, whole genome shotgun sequence.
ACCESSION   DATCQR010000254 DATCQR010000000
VERSION     DATCQR010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199586"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            133..753
                     /gene="upp"
                     /locus_tag="VIN32_08345"
     CDS             133..753
                     /gene="upp"
                     /locus_tag="VIN32_08345"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEY8922623.1"
                     /translation="MPLRVSKHPLVADSLAGLRDRATEPEEFRELARKVITLLLYEAT
                     ADLPVRNGTVQTPLRESAATFVESEVVAIPVLRAGLGILGPALELLPRVSVGYIGLER
                     DEETAVARIYYQKLPSLAGKIPLLLDPMLATGGSAAQALDLIKEAGGRDPRMICIVAA
                     PEGVKVLEERHPEVQIFTAALDEGLNDRSFIVPGLGDFGDRLFGTG"
     gene            complement(750..1760)
                     /gene="add"
                     /locus_tag="VIN32_08350"
     CDS             complement(750..1760)
                     /gene="add"
                     /locus_tag="VIN32_08350"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HEY8922624.1"
                     /translation="MRDPGQVRHRLRPRLRRALPRAAWHLLAVIGKYTAWRKAELHCH
                     LDGAVRSSTAEELAVEQGLDLKRPLRMTAPEDCPSQAVYISYFDDAIAVLQTARALER
                     AALELGIDSAEENIDYLEVRWAPRLHLRNGLTVQEVIEAVLSGLSAAPLRAVAIVCAM
                     RHHSVEDNVVLAKEAGLFAGRGVVGFDLAGDEIRYPAAPQRPAFEAARAAGLRLTCHA
                     GEAGDPLNVEEALDLGVERIAHGVIGAREPRIVERIRSEGVVLDLCPTANWKCKAVPT
                     LARHPLPALVRAGVRCTISTDSRTVADTTLSQEFERASAMGLSDDELKGCNETAYAAR
                     FG"
     gene            complement(1670..>2099)
                     /gene="hpt"
                     /locus_tag="VIN32_08355"
     CDS             complement(1670..>2099)
                     /gene="hpt"
                     /locus_tag="VIN32_08355"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEY8922625.1"
                     /translation="IDTPLVMVVILKGATVFAADLLRSLTIPAELEFMRAASYGSGTS
                     GGRLRLAHMVEGPLVGRHVLLVEDIVDSGRTIGAIKKRLNRLGPASLRLAALLDRPAR
                     REVEVEIDFTGFVIPDKFVIGYGLDYAGLYRELPGIYSLS"
BASE COUNT          368 a          707 c          674 g          350 t
ORIGIN      
        1 ttcgggctgg cactctgaac ttatgcgtcg acgagatggg tgcgggcgag atacaccacg
       61 tggacccatc cttcatggac cgcatgttca aggtcgcggt ggacgcgatc cggcgcctca
      121 tcgagatgga tcgtgccact aagggtcagt aagcatccgc tggtagcgga cagccttgcc
      181 ggtcttcgcg accgcgccac cgaacctgaa gagttccgcg agctggccag gaaggtgatc
      241 acgcttttgc tttacgaggc aactgcggat ctaccagtgc ggaatggaac tgtccagacg
      301 ccgctcagag aatccgctgc cacgttcgtc gagagtgagg tcgtcgccat ccccgtcctt
      361 cgggcaggcc tggggatcct cggcccggcc ctggagctct tgcctcgagt cagcgtcggc
      421 tacatcgggc tcgagcgcga cgaggagacg gcagtcgcgc ggatctatta ccaaaagctg
      481 ccaagcctcg ccggcaaaat cccgctgctg ctggatccga tgctcgcgac cgggggctcg
      541 gcggcccagg cgctggacct gatcaaggag gccggcggcc gcgaccctcg catgatctgc
      601 atagtggcgg cgccggaggg ggtcaaggtg ctcgaggaac gccaccccga ggtccagatc
      661 ttcacggcag ccctcgacga aggcctcaac gatcgatcct tcatcgtccc ggggttgggc
      721 gacttcggcg accgcttgtt cggtaccggt tagccgaacc tcgccgcgta agcggtttcg
      781 ttgcagccct tcagctcgtc atcgctcagg cccatcgcgg atgcacgctc gaactcctgg
      841 ctcaaggtcg tgtcggcgac agttcgcgaa tccgtgctga tggtgcagcg caccccggct
      901 cggaccaacg ccggcagcgg gtgccgcgcg agggtgggga ccgccttgca cttccagttg
      961 gcggttgggc agaggtcgag gaccacgccc tctgatcgaa tcctctccac gatgcgcggc
     1021 tcgcgagccc cgatcactcc gtgcgcaatg cgctccacgc ccaggtccag cgcttcctcg
     1081 acgttcaacg gatcgcccgc ctctcccgca tggcatgtga gcctcaggcc cgctgcccgc
     1141 gcagcctcga atgcaggccg ctgaggcgct gccgggtagc ggatctcgtc gccggcgaga
     1201 tcgaaaccga cgacaccgcg accggcaaac agccccgcct cctttgccag cacgacgttg
     1261 tcctctaccg agtggtggcg catggcgcag acgattgcca ctgcacgcaa gggagcggcg
     1321 ctgagcccag acaggactgc ctcgatcacc tcttgcaccg tgaggccgtt gcgcaagtgg
     1381 aggcgcggcg cccagcgtac ctccaggtag tcgatgttct cctctgccga atcaattccg
     1441 agctccagcg cggccctctc gagcgcgcgc gccgtctgca ggacggcgat cgcgtcgtcg
     1501 aagtaggaga tgtaaacggc ctgcgaagga cagtcctcgg gcgccgtcat gcgcagcggc
     1561 ctcttgaggt cgagaccctg ctccacagcg agctcttcgg cggtcgacga acgcaccgcc
     1621 ccgtcgaggt ggcagtgcag ctcagcctta cgccaagccg tgtatttgcc tatgacagcg
     1681 agtagatgcc aggcagctcg cggtagagcc cggcgtaatc gaggccgtag ccgatgacga
     1741 acttgtccgg gatcacgaat cccgtgaaat cgatctccac ctcaacctca cgacgggcgg
     1801 gccggtcgag gagcgccgcc aggcgcagcg aggcaggccc cagccgattg agccgcttct
     1861 tgatggcgcc aatcgtccgt cccgagtcga cgatgtcctc gaccagcagg acatgacgtc
     1921 cgaccagcgg gccttcgacc atgtgcgcca gccgcagccg cccgcccgag gtgcctgagc
     1981 catagctcgc tgcgcgcatg aactccagct cggcgggaat ggtcaggctg cgcagcaggt
     2041 cggccgcaaa gaccgtggcg cccttgagga tgacgaccat caccaaaggc gtgtcgatg
//
LOCUS       DATCQR010000255         5113 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_106994, whole genome shotgun sequence.
ACCESSION   DATCQR010000255 DATCQR010000000
VERSION     DATCQR010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5113
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106994"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            54..371
                     /gene="groES"
                     /locus_tag="VIN32_08360"
     CDS             54..371
                     /gene="groES"
                     /locus_tag="VIN32_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEY8922626.1"
                     /translation="MSASTPVASETKLKPLGDRLVVRPSAREEMTKSGIVLPDTVKER
                     PQEGTILAVGPGRTLDDGSREPIEVSVGEKILFQKYAGTEFKLDDEDLLILSQKDVLA
                     VIK"
     gene            393..2021
                     /gene="groL"
                     /locus_tag="VIN32_08365"
     CDS             393..2021
                     /gene="groL"
                     /locus_tag="VIN32_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009349866.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEY8922627.1"
                     /translation="MAKQLLFDEEARRALKRGVDKMADAVKVTLGPRGRYALLDKKYG
                     SPTITNDGVTIARDIELEDPYENMGAQLLEEVAAKTNDIAGDGTTTSIVLAQAIISEG
                     LRNVTAGANPMALKRGIERGVTAVVDEIKRLSKPVLTKDDIAHIAAISARDTEIGEMI
                     AEVMEKVGKDGVVTVEESQGLKLDKEYVEGMQLDRGYVSAYMVTSNDAGRMEATLEAP
                     YILVTDKKISAVADILPTLEKVVGAGRPLLIVAEDVDGEALATLVVNKLKGTISVLAI
                     KAPGFGDRRKAMLEDIGTLTGARVISEEVGRKLESVALEDLGQARRVTATKDTTTIVE
                     GKGDANAIEARKSQIKLQIDDTTSDFDREKLQERLAKLSGGVAILKVGAATEVELKEK
                     KHRIEDALSAARAGVEEGMVAGGGSVLLHAIPALNKVEALGDEATGVNILRRALEEPL
                     RQIAANGGWEGSVVVEAVRKLKPGNGWDAQKAEYTDMFVAGIIDPAKVTRSALENAAS
                     VAALLLTTETVVTDLPEKEKAPMPGMGGGGMGDY"
     gene            complement(2230..2901)
                     /locus_tag="VIN32_08370"
     CDS             complement(2230..2901)
                     /locus_tag="VIN32_08370"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY8922628.1"
                     /translation="MGSRDDRRTIGRLKGERLARQLTAEWRELRLGAGVSQSAVSQTV
                     GISRSAYARLERGEATEIGVVRAAIVTAALGGDLSVKVYPGGPPIRDAGHVALLAMLD
                     ARVSGRWRVTHEAPVQAGDGRAWDRRLDGPVSIGLEAEVALRDLQALERRMQRKKEDS
                     GVTRMILLVRGTRRNREVLRELLPSVRGTFPLGSRELLRALADGRDPGADGLLVLDAP
                     FARAT"
     gene            3117..>5113
                     /locus_tag="VIN32_08375"
     CDS             3117..>5113
                     /locus_tag="VIN32_08375"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEY8922629.1"
                     /translation="MSPQPTTPATLSPEEILARLNEPQREAVSHTGGPLLILAGAGSG
                     KTRVLAHRVAYLVATGTKPWQIVAVTFTNKAANEMRERIAGLIGEEGAREATIGTFHA
                     ICARILRRDGNAIGLTRSFTIYDRADQVAMVKGILRRLDLDEKRFAPAGMLAWIGQRK
                     DELADPATATRQAANYYDETAARVYAAYQEQLNEDDAVDFDDLLMRAVFLFEQHPEIL
                     ARYQGRWQQILVDEYQDTNRAQYLLCSLLAAKHGNLAVVGDDDQSIYSWRGADLRNIL
                     DFERDHPTTKVVKLEQNYRSTQTILDAAHAVVSRNEGRKEKKLWTDRGAGTAITLFDA
                     YNEYEEAEFVARQVERLTGSGRGGLNAILTARADDEAGSLRYGDIAVTYRINAQSRVL
                     EESFVRFGIPYQLVGGTRFYERREVKDALGYVRLSRNTSDRVALGRVVNVPARGIGDK
                     TLEELRASAEARGGTLWDAIERAGENPNLSPRARTALSGFAELVRGFTAIAAKEPPSA
                     IFDAVYERTGLREQLQDGTDDGEERWTNLLELRNHAAEFDELAIPEGLERFLEEVALV
                     SDQDELEDRPERVTLITLHAAKGLEFPVVFIVGLEEGLLPHRRALEDDRELEEERRLA
                     YVGMTRAKDRLYLVHANHRSTWGVGAASEPSRFLAELPEDLL"
BASE COUNT          934 a         1739 c         1724 g          716 t
ORIGIN      
        1 attcttgaca cgcggcttcc tgccgggcta cgatccgtta gcactctcag cggttgagtg
       61 ccagcacccc ggtcgcatcg gagaccaagc tcaagcccct cggcgaccgc ctggtcgtca
      121 ggcccagcgc ccgcgaggag atgaccaaga gcggcatcgt cctgccagat accgtcaagg
      181 agcgccctca ggaagggacg atcctggcgg tcggaccggg gcgcaccctg gacgacggca
      241 gccgcgagcc gatcgaggtc tcggtcggag agaagatcct gttccagaag tacgccggga
      301 ccgagttcaa gctggatgac gaggatctcc tgatcctctc ccagaaggac gtcctggcag
      361 tcatcaagta gtttcggagg acgaacaaca tcgtggcaaa gcagctcctg ttcgacgaag
      421 aggcacgacg cgcgctgaag cgcggggtcg acaagatggc cgacgccgtc aaggtcaccc
      481 tgggcccccg cggccggtat gcgctcctgg acaagaagta cggatcgccg acgatcacca
      541 atgacggcgt caccatcgcg cgcgacatcg agctggagga cccctacgag aacatgggtg
      601 cccagctgct cgaggaggtc gcggccaaga ccaatgacat cgccggtgac ggcaccacca
      661 ccagcatcgt gctggcccag gcgatcatca gcgagggcct gcgcaacgtg accgccggtg
      721 ccaacccgat ggcgctcaag cgcggcatcg agcgtggcgt gaccgcggtc gtggacgaga
      781 tcaagcgact ctcgaagccg gtcctcacca aggacgacat cgctcacatc gcggccatca
      841 gcgcccgtga caccgagatc ggcgagatga tcgccgaggt catggagaag gtcggcaagg
      901 atggcgtcgt caccgtcgag gagagccagg ggctcaagct cgacaaggaa tacgtcgagg
      961 ggatgcagct cgatcggggc tacgtcagcg cctacatggt cacctcgaac gacgcaggcc
     1021 gcatggaggc caccctcgag gcgccctaca tcctcgtcac cgacaagaag atcagcgccg
     1081 tggccgacat cctgccgacc ctcgagaagg tcgtcggcgc cggcaggccg ctcctgatcg
     1141 tggccgagga cgtggacggc gaggcactcg ccaccctggt cgtcaacaag ctcaagggca
     1201 ccatcagcgt gctcgcgatc aaggcacccg gcttcgggga tcgccgcaag gccatgctcg
     1261 aggacatcgg caccttgacc ggtgcgcggg tcatctccga ggaggtcggg cgaaagctcg
     1321 agagcgtggc gctcgaagac ctcgggcagg cgcgtcgagt gaccgctacc aaggacacca
     1381 ccaccatcgt cgaggggaag ggcgacgcga acgcgatcga ggcccgcaag tctcagatca
     1441 agcttcagat cgacgacacc accagcgact tcgaccgcga gaagctccag gagcgcctgg
     1501 ccaagctctc cggcggcgtg gcgatcctca aggtcggcgc ggccaccgag gtcgagctga
     1561 aggagaagaa gcaccgcatc gaggacgcgc tctcggcagc ccgtgccggg gtcgaggagg
     1621 ggatggtcgc cggtggcggc tcggtcctgc tccatgcgat ccccgcattg aacaaggtcg
     1681 aggccctcgg agacgaggcg acgggcgtca acatcctgcg ccgtgcgctc gaggagccgc
     1741 ttcgccagat cgccgcgaac ggcggctggg agggatcggt cgtcgtcgag gcggttcgca
     1801 agctgaagcc tggcaatggc tgggatgccc agaaggccga gtacaccgat atgttcgtgg
     1861 ccggcatcat cgacccggcc aaggtcaccc gctcggcact cgagaacgcc gcatcggtgg
     1921 cagccctgct gctcaccacc gagacggtcg tcaccgatct gccggagaag gagaaggcgc
     1981 cgatgcccgg catgggcggc ggcgggatgg gcgactacta acccgtcctc cacacaccgt
     2041 taggagagcc ccggcttcgg ccggggctct ttcgttggtt cctggcagcg aacgaccacg
     2101 cggttgtcat tgccgacacc tcatcggtct ttatgaccat ccggcgggcc aaatctgcat
     2161 ccggctggct catatgacag cccagtcgtc agacgggcgc ccgggctgac agcagtgacg
     2221 cctcagtggt caggtcgcac gagcgaacgg ggcgtcgagg acgagcaagc cgtcagcacc
     2281 ggggtcgcgg ccatcggcca gcgcacggag gagctcccgt gagccgaggg ggaaggttcc
     2341 acgcacggag ggaagcagct ctcgcaggac ctcgcggtta cgccgcgtcc ctcgcacgag
     2401 caggatcatg cgagtcacgc cgctgtcctc cttcttgcgc tgcatcctgc gctccagcgc
     2461 ctggaggtca cggagcgcca cctcagcttc caggccgatc gagaccggtc cgtcgaggcg
     2521 gcggtcccac gcccgtccgt caccggcctg aaccggtgcc tcgtgtgtga ctcgccaccg
     2581 gccactgacc cgcgcgtcga gcattgccag cagcgccacg tgccctgcgt cccggatggg
     2641 cggtccgcct ggatagacct tgaccgacag gtcgcccccg agcgcggcgg tgacgatggc
     2701 cgcccgcacc actccgattt ccgtagcctc gccgcgttcg agccgagcat atgcggaccg
     2761 cgagattccg acggtttgag aaacggccga ttgcgagact ccggccccaa gccgcagctc
     2821 gcgccactct gccgtgagct ggcgggccag gcgctcaccc ttgagccggc caatcgtgcg
     2881 tcggtcgtcg cggctgccca tgacgggatg gtcatgtacg cggccttacg gcgcgcttat
     2941 ccgccaatga tcgccgggtg atcaatacga ctagccatgc gtcgctggtg accacccagc
     3001 acgccatcgg gcgggtcgag tgctcaagct gacgatccgg cgagccagat ctgccaccca
     3061 atgctctgat tgaccgcgag gcggtcacgg gccgtcgacc ctagaatcag gagccgatgt
     3121 ccccccagcc cacgaccccc gcgaccctca gccccgagga gatcctcgca cgcctcaacg
     3181 agccgcagag ggaggcggtg tcgcacaccg gcgggcccct tctcatcctc gccggggccg
     3241 ggtccggcaa gacgagagtc ctcgcgcatc gggtcgccta cctggtcgcc acgggcacca
     3301 agccgtggca gatcgtggcg gtcaccttca ccaacaaggc ggccaacgag atgcgcgagc
     3361 ggatcgcggg gctgatcggg gaggagggag cgagggaggc gacgatcggc accttccacg
     3421 ccatctgtgc gcgcatcctg cgccgcgacg gcaacgccat cggcctcaca cggtccttca
     3481 cgatctacga ccgggccgac caggtggcga tggtcaaggg gatcctgcgt cggcttgacc
     3541 tcgacgagaa gcgattcgcg cctgcgggca tgctcgcctg gatcggtcag agaaaggacg
     3601 agctggccga cccggccacc gcaaccaggc aggccgcgaa ctactacgac gagacggcgg
     3661 ctcgtgtcta cgccgcctac caggagcagc tcaacgagga cgacgcggtc gacttcgacg
     3721 acctgctgat gcgcgcggtc ttcctgttcg agcagcaccc tgagatcctt gcccgctacc
     3781 aggggcgctg gcagcagatc ctggtcgacg agtaccagga caccaaccgg gcgcaatacc
     3841 tgctctgcag cctgctggcg gcgaagcacg gcaacctggc ggtggttggc gacgacgacc
     3901 agagcatcta ctcgtggcgc ggcgcggacc ttcgcaacat cctggacttc gagcgcgacc
     3961 accccaccac caaggtcgtg aagctggagc agaactaccg ctccacgcag accatcctgg
     4021 acgctgccca cgcggtcgtc tcgcgcaacg aggggcgcaa ggagaagaag ctgtggaccg
     4081 atcgcggggc cggcaccgcg atcaccctct tcgacgccta caacgagtac gaggaggccg
     4141 agttcgtggc gcgccaggtc gagcgcctga ccgggtcggg gcgcggaggg ctcaacgcca
     4201 tcctcaccgc ccgggccgat gacgaggcag gctccctgcg ctacggcgat atcgccgtca
     4261 cctaccgcat caacgcccag agccgggtac tggaggagtc cttcgtccgc ttcgggatcc
     4321 cgtaccagct ggtcggcggc acccgcttct acgagcggcg cgaggtcaag gacgcgctcg
     4381 gctacgtccg cctgtctcgc aacacctcgg atcgggtggc actggggcgg gtcgtcaacg
     4441 tcccggcgcg cgggatcggc gacaagaccc tggaggagct gcgcgcatcc gccgaagccc
     4501 gcggcgggac gctgtgggat gccatcgagc gcgccggcga gaacccgaac ctgtcgccgc
     4561 gcgcccgtac cgcgctctcc ggcttcgccg agctggtgcg cggcttcacg gccattgccg
     4621 ccaaggagcc gccatcggcc atcttcgacg cggtctacga gcgcaccgga ctgcgcgagc
     4681 agctccagga cgggaccgat gacggcgagg agcgctggac caacctgctc gagctgcgca
     4741 accacgccgc cgagttcgac gagctggcaa tcccggaggg gctcgagcgc ttcctggagg
     4801 aggtcgcgct ggtgagcgac caggacgagc tggaggaccg acccgagcgg gtgacgctca
     4861 tcaccctgca cgccgccaag gggctggagt ttccggtcgt cttcatcgtg gggctggagg
     4921 aagggctgct gccgcatcgc cgcgccctcg aggatgaccg cgagctggag gaggagcggc
     4981 ggctcgccta cgtgggcatg acgcgggcca aggatcggct ctacctggtg cacgccaacc
     5041 atcggtccac gtggggcgtg ggagcggcca gcgagccgtc gcgcttcctg gccgagctgc
     5101 cggaggacct gct
//
LOCUS       DATCQR010000256         3597 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_123402, whole genome shotgun sequence.
ACCESSION   DATCQR010000256 DATCQR010000000
VERSION     DATCQR010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3597
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123402"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            431..1312
                     /locus_tag="VIN32_08380"
     CDS             431..1312
                     /locus_tag="VIN32_08380"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018636731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEY8922630.1"
                     /translation="MTLGRIGVWTNTLDAQPALKAQEATQELERLGYAAIWFPESVRR
                     EALSNAGLLLAATSRIVVATGIASIYARDAMTMAAGQKTLAEAYPGRFLLGLGVSHQP
                     NVEQLRGHIYGKPVATMRAYLDAMSTAPYQSVEPSEKPQTVLAALGPNMLKLAAELTS
                     GAHPYFVPVEHTRRAREALGPKAILAPEQAVVLETDANRARAIARSHTQHYLRLPNYV
                     NNLHMLGYTEEDVTGGGSNRLVDEIVAWGDLDLVTSRIRAHFEAGADHVCVQVIAEDQ
                     KALPMEAWRALAEQLLT"
     gene            1724..2665
                     /locus_tag="VIN32_08385"
     CDS             1724..2665
                     /locus_tag="VIN32_08385"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: protein motif:HMM:NF015047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEY8922631.1"
                     /translation="MTEAARKLCQLLGIDVPILQAGMGAATSPALVAAVSNAGGLGII
                     GCLRRGPDEVRQLIAQITAGTTRQFAANHVIAHLNGAALEASLEAGAPLISTAWGDPA
                     AVVQVAHAAGALVIHQVNTVEQAVSARDVGVDVVVAQGNEGGGHVGHLATFSLVPQVV
                     DAVAPLPVLAAGGIADGRGVAAALMLGAAGVLVGTRFLATEEAPVAPAWKEAIRCAGG
                     DQTVTTGFYDAMRAEVWPGAVVRAIRNRWTDEWGGREGEWVQVAEELRPEFLRSFAAG
                     EFPMAGQAIGLISDVVPAGGLVQRLWQEGSALLHPED"
     gene            complement(2985..3257)
                     /locus_tag="VIN32_08390"
     CDS             complement(2985..3257)
                     /locus_tag="VIN32_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018187744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEY8922632.1"
                     /translation="MPKHENNHQVQGQLRRIEAKVRGLQKMIDDHRYCVDVLTQINAV
                     KEDLETVALLLMADHTEHCVAEVISGGDAGVKVRELNVAVERLLRG"
BASE COUNT          575 a         1178 c         1237 g          607 t
ORIGIN      
        1 actatgtgcc atcggacggc cgggacgtgg cactggcaaa gctggagcag ctggcggaga
       61 tggccaggcc atacatgccc tgatcccggg cggcgtctcg gttcacgtgg cgggagtggc
      121 cgcggccgac gaggtgacca ccgagggccg cctgatcgtc gagctgcccg cgtcgtgagc
      181 cgcgtccggc gcaggatcgc aggcagtcga tgtgctgctc atcatcggcg ggggcagtgc
      241 cggcctctgc gccgccctgt cggcccgcga gagtggcgcg ggtgcttcgg cgatggcgtt
      301 tcgccttcgg tgatcgcggc ggtgtccgca gcgctggcct ctcccaatca cgcgcctacg
      361 attggtgcag catgccgacc ttcgccgccg cagaacgcca agtcgcgtgg aatcgggccg
      421 gggctaacta atgacactcg gccgtatcgg cgtttggacc aacacgctcg acgcacagcc
      481 ggccttgaag gcgcaagagg ccacccagga gctggagcgg ttgggctacg ccgccatctg
      541 gttcccggag tcggtacggc gcgaggccct cagcaacgcc ggcctgctgc tagccgcgac
      601 cagccggatt gtggtggcga ccgggatcgc cagcatctac gcgcgcgacg ccatgaccat
      661 ggcagccggg cagaagacgc ttgccgaagc ctacccgggc cgcttcctgc tcggcctcgg
      721 ggtcagccac cagcccaatg tcgagcagct gcgaggccac atctacggca agccggtggc
      781 caccatgcgt gcctacctgg acgctatgtc gacggctcct taccagtcgg tcgagccgtc
      841 cgagaagccg cagacggtcc tcgccgcact cgggccaaac atgctgaagc tggcggcgga
      901 gctgacctca ggagcccatc cctacttcgt cccagtcgag cacacgcgcc gggcccgcga
      961 ggcgcttggc ccgaaggcaa tactcgcgcc ggaacaggcg gtggtgctgg agacggacgc
     1021 aaacagggcg cgggccatcg cccgcagcca cacgcagcac tacctccgcc tgcccaacta
     1081 cgtgaacaac ctgcacatgc tgggctacac cgaggaggac gtcaccggcg gaggcagcaa
     1141 ccgcctggtg gacgaaatcg tggcctgggg cgacctggac ttggtgacct ctcggatccg
     1201 cgcccacttt gaggcggggg cggatcatgt ctgcgtgcag gtcatcgccg aggaccagaa
     1261 ggcgctgcct atggaggcgt ggagggctct ggctgagcag ctgctgacct agcgccggcg
     1321 cacccgctag ctccctgttc gaggtggagc tggtggcgga gaacgaggtt ctcaagggcg
     1381 acgttacgcc gggaccggaa tgccggcaag cagactccgg agcagtagga cgatgagatg
     1441 gagcatccgg caaggtaccg tccgatgccg gtagccgatc gagttggagg gtgtttgccc
     1501 cccttcaacc agaggaatgc gtggactagt aaaagacacc ccttcattga ccccgggcgg
     1561 caacttggtt cgggcgggaa gggcggtcga gccaggcccc aatcgggttg ttgcaatcgt
     1621 gcggggggag catgatggcc ggcatgagag tccggctgct catgtggatt ggggccgggg
     1681 ttgccgtcct ggccgtggct gggtccaggt ctatgatcgg gccgtgaccg aggcggcccg
     1741 gaagctctgc cagctcctcg gcatcgacgt cccgatcctc caggccggca tgggcgcggc
     1801 cacgtcgccg gcccttgtgg ctgcggtcag caatgccggc ggccttggca tcatcggctg
     1861 cctgcgccgc ggtcctgatg aggtccggca gctgatcgcc cagatcacgg cggggactac
     1921 gcggcagttc gccgccaacc acgtgatcgc gcacctcaat ggcgccgcgc tcgaggcgtc
     1981 gctggaggcg ggcgcccccc tcatctccac tgcgtggggc gacccggcgg cggtggtgca
     2041 ggtggcgcac gccgccgggg ccctcgtcat ccaccaggtc aacaccgtgg agcaggccgt
     2101 cagtgctcgc gacgtggggg tggacgtggt ggtcgcccag ggaaacgagg ggggaggcca
     2161 cgtcggccac ctggccacat tctcactggt cccccaggtc gtcgacgcgg tggcgccgct
     2221 cccagtgctg gcggccggtg ggatcgcgga cggccggggt gtcgccgcgg ccctcatgct
     2281 gggcgcggcc ggggtcctgg tggggacgcg ctttcttgcc accgaagaag cccctgtcgc
     2341 tccggcctgg aaggaggcga tccgctgcgc gggcggcgat cagaccgtga cgaccggctt
     2401 ctacgacgcc atgcgcgccg aggtctggcc gggggcggtg gtccgcgcga tcaggaaccg
     2461 ctggaccgat gagtggggcg gccgcgaggg cgagtgggtg caggtggccg aggagcttag
     2521 gcccgagttc ctccgctcct tcgccgccgg cgagttcccg atggcgggcc aggccatcgg
     2581 actgatcagc gacgtggttc cggccggcgg cctggttcag cgcctctggc aggaggggtc
     2641 ggctctgctc cacccagagg actgacgctg gaagggcggt gcgggacgcc tgccgggtcg
     2701 gtttcgtggc cgaggagctg gtcgtcgtcc acgccatcct cggctccgac gttggcctct
     2761 tctccttcaa gcccggagtc gaccgtcctc accgcagcct ggtcgccaga gtcccgtgcc
     2821 attcgaagcc aaactggcga cggaacggtt gtcaggcgac cggagttaga aggtctcccc
     2881 cacgtctacg acagggcagc atgagttcgg atcacgtttt gccccccctt cagcacccca
     2941 caccatctgg agtgcctggc cgactgccaa tatgaccgac atcgttaccc cctgaggagg
     3001 cgctcgacgg ccacgttcag ctcgcgcacc ttcacgccgg catccccgcc actaatcact
     3061 tcggccacgc agtgctcggt gtggtcagcc attagaagca gggcaacggt ctctaggtct
     3121 tccttcaccg cgttgatctg ggtcaaaacg tcgacgcagt agcgatggtc gtcgatcatt
     3181 ttctggaggc cgcggacctt tgcctcgatg cgcctgagct ggccctgtac ttggtggttg
     3241 ttctcgtgct taggcatgtc tatgactaga cctccaagag ttacaccact atcgccgcga
     3301 gcttgccgct ttatcgctcg tcgtgctcat ttccgacgtg ctccgcgcag ttggctcggc
     3361 cgtccacgct tggtacacgc cccacccgat tccggctgcc aaaacggcca acgcggcagc
     3421 ttgggcgatt acgggcaaac ccctgagcgc agccgccaga tctcccgtca gaccgatggc
     3481 ggcattgtcg ggtggcattc tcaaattgtc aagagatcaa ttgacttctt gacacatacg
     3541 ggcgataagg cgatactggt gagccatgac ggatcgtgcc ttgaagatcg agctctt
//
LOCUS       DATCQR010000257         8927 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_123430, whole genome shotgun sequence.
ACCESSION   DATCQR010000257 DATCQR010000000
VERSION     DATCQR010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 8927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8927
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123430"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..624
                     /locus_tag="VIN32_08395"
     CDS             <1..624
                     /locus_tag="VIN32_08395"
                     /inference="COORDINATES: protein motif:HMM:NF020926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1998 domain-containing protein"
                     /protein_id="HEY8922633.1"
                     /translation="ERKAYVTRTDVDYYTDADLGITLKVLEVFEEADEPPAGMRQRGE
                     VMVAWQVTMFKKIKYHTHENVGWGSISIPEQEMHTTACWLVPPAELVNRYDRDTLDGA
                     LIGLARVARTTASLLLMCDPRDLGVQAQVQSPFTGRPTVYVYDAVPGGVGLTERLYTL
                     TDELVRACREAVDSCACTDGCPACVGPAIEVGSRGKATVSELLAGLS"
     gene            628..1854
                     /locus_tag="VIN32_08400"
     CDS             628..1854
                     /locus_tag="VIN32_08400"
                     /inference="COORDINATES: protein motif:HMM:NF024873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HEY8922634.1"
                     /translation="MRTLADRLAEMRAERGKPAPPRPARLPDNRAEVLARWFGARLQV
                     APDGAAVVVERRVSLPPATVANLAGLPSACYFDTETTGLSTGAGTVVFLAGLGHLEGS
                     QLVVRQLLLPDYPHEAALLRIACGELAALPRVVTYNGRGFDLPLLITRLTVHRLFREM
                     SALPELHDDLLPVARRLYRRPLGGARLADVESGVLGVIRTSDCPGSEVPSRYFGYLRG
                     GSPDLLTDVLDHNLQDIVSLALLEAEIVRMRAGGWRDAPVLDPHGMAVELLRHGASDE
                     ALEVVEAAMLPGVDQDRAHSLRRMAARLLISAGQVDRAEELWTAATRRASTDAAAAWL
                     EVARIRERQRGDLHGALEAALTSSRVLDLAFALGRGGGMLAVGRVRLRVDARLRRLRR
                     WVAAADRRAAREPRVA"
     gene            2007..2330
                     /locus_tag="VIN32_08405"
     CDS             2007..2330
                     /locus_tag="VIN32_08405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8922635.1"
                     /translation="MKLFFYELHEGATDLLTDAVLVSDQEYAPEAFASLVLAARAAVV
                     DTFEEDTLVEAIARELERSHGFSYAGDEKLTASMSVGMDEADTYLVETSDEFRSVIAE
                     VDLSS"
     gene            complement(2339..3430)
                     /locus_tag="VIN32_08410"
     CDS             complement(2339..3430)
                     /locus_tag="VIN32_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HEY8922636.1"
                     /translation="MQVTIVGLPGSGKTTVFNALTGAHADTGGFSGGRAAPNMGVVKV
                     PDERLDRLAELFHPKKTTPADVTYVDVAIPAGATREGTINPDVLALVRNADALLHVAR
                     AFDGGAEGPPDPWRDIEALDLEFAIADLMVIEKRLEKLRTSGRHGSPAEREANAQEEA
                     LLDRLEPELSAGQPLRAVALSDEDELRLRGYRFLTQKPALVVLNVDEGQLGAAAELEA
                     AGRTRYGQPQTDVAALGGKIEAELTQLSEADAALFMEDLGIAEPSRGRVIHLTYALLG
                     LFSFFTAGEDECRAWTLRRGATAVDAAAAIHTDLARGFIRAEVVTYDDLIACGSMAEA
                     RKRGLLRSEGKTYAVRDGDVIEVLFNVAR"
     gene            complement(3434..4327)
                     /locus_tag="VIN32_08415"
     CDS             complement(3434..4327)
                     /locus_tag="VIN32_08415"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HEY8922637.1"
                     /translation="MQISREGLVHDDAEAALASARVLLRGGVPASVLDHIIARAPRLE
                     WIHSFSAGVDRVATPAVRERGLVVTNARGVFSRPIAEYVVMMCLAIARRLPQLLELQR
                     ERTWQPLRGTELGGMTVGIVGFGSIGSEIAKLLAPFEAKVLATRRHPDRGAGEASNVE
                     LLGLDQLDELLRRSDIVVVAAPLTDDTAGLIGSAQLQEMPEHSWLINIARGRLLDELA
                     LRRALESGWIGGAVLDVFNEEPLPPDSPLYGTPNLIITPHTSWSSDRVVDRSLDLFVA
                     NLRRFAAGEPLENVVDLEAGY"
     gene            complement(4423..5595)
                     /locus_tag="VIN32_08420"
     CDS             complement(4423..5595)
                     /locus_tag="VIN32_08420"
                     /inference="COORDINATES: protein motif:HMM:TIGR03624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HEY8922638.1"
                     /translation="MARSASRIGMGIVVGGLLAMGATAVQRELLRRAGTRLIDWEAVR
                     RIARRRLGPEGAALSAADRSAAEEFYRAELLRIEPIVAEAIGAELPRALETPAVVDRF
                     EWIDLNLATFEQLFGRVERIINDATTGADTPGRALARILNRSFGNQQLGLLMAFLARK
                     VLGQYDVSLLAAAPAARGRLNFVEPNIRASAATLGVSLGEFRTFVALHEATHAFEFEA
                     YPWLRDHFAASVGEAIEQMATDTGGLGQRLRGALRGRDGHWLERMMTPAQLSTFRRTQ
                     ALMSLLEGYSNHVMNDAGERLLPGFTKLHERFERRNEARGALERAIMRLTGLDLKMEQ
                     YAAGERFVKAVLAVRDRAFLNQVWEGPANLPDLAEIRYPQGWIDRMEGRGAREQVS"
     gene            complement(5605..6429)
                     /gene="murI"
                     /locus_tag="VIN32_08425"
     CDS             complement(5605..6429)
                     /gene="murI"
                     /locus_tag="VIN32_08425"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEY8922639.1"
                     /translation="MGLRTDPRPIGVFDSGVGGLTVLAELRRRLPAESTLYLGDNARA
                     PYGPRSADEVRAFTLESVAWLLSQDVKLLVLACNTATAQALPMVRELAAVPVLGVVRP
                     GALAAAASTRVGRVGVIATAGTVASGAYPTAIGEADPALLVTQLACPQLVPMVEAGEL
                     DGPVAEVTVGGYLTELFGMDREIDTLLLGCTHYPLLRPLIERLTGPGVAVVDSAFTTA
                     LATEDLLDALGGRTASGGPGDNRIVTTGDVGTFSAVAATVFGAALPSVEQAVVAAV"
     gene            complement(6438..7583)
                     /locus_tag="VIN32_08430"
     CDS             complement(6438..7583)
                     /locus_tag="VIN32_08430"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8922640.1"
                     /translation="MAEQIRLLVIEDVPQVAMHMRSLLQAQSQIKMLDVITSGEQALS
                     AVTELRPDVLIVDALLQGRVSGMQVAEQVRRTEPTVSVIVLTVPQNPVTEDPAAGIDA
                     VLKMPFSGFDLTTMVRKVHEQSNVAGGHNASVMVSLFSPKGGVGRTTLAFNLAVALSA
                     DHQVCLIDGSLQFSDMRGLLRAPAAAPSIMNLPTDRIRDTDLAEVLWKDPSGIDVLLA
                     PPRIEMAEMVTVRDIEKALSIIRQVYEVIVVDTRASLSDDVLAFLDASDLILEVLAYD
                     SMAIRALAMADEAFATIGYAPSKIEAVLNRSDSTGGFSKADVEEALNRQIDHEVVSDG
                     KLVIAANNEGVPFVLANPDAQVSQGIRAMAASIAARLPARAGVLARR"
     gene            complement(7576..8421)
                     /locus_tag="VIN32_08435"
     CDS             complement(7576..8421)
                     /locus_tag="VIN32_08435"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY8922641.1"
                     /translation="MSADIFGSQPADEDRLAELYDLEHDELVEDLPFYRELARRTDGP
                     VLDLGCGSGRLFRTLLDAGASRILGLDGSAALLRRAEARIAADEGLAQAARDGRLSVM
                     RGDVRAISTLNIRERFDLVVAVGVLPHLDGPEDALRLLGGTRSVLAKGGRLVIDDLGP
                     AEMPVRDMPLSIDWRRQMDGREVVRRSELVRRESPEGLRVAFTTIADAVQPDGTIARL
                     PASHQLWYPSPEALAALVKQAGMVVDLSYGSHDLEPLGRESGRRIYVVAYEFAAAPSH
                     EVKVG"
     gene            complement(8435..8842)
                     /locus_tag="VIN32_08440"
     CDS             complement(8435..8842)
                     /locus_tag="VIN32_08440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8922642.1"
                     /translation="MTSRSRIAVAALVLLAVLGAVVLVAFAGNACAGDLPGQPCPNAG
                     VNRAAVVALAALTAGVAVMPFALLAEFAVRRRIIYRGAWGRAIRRGVLVGAVIAAVAG
                     LRLGGALSVPVALFVVSLAAATEWIALRRFDLP"
BASE COUNT         1377 a         3166 c         3081 g         1303 t
ORIGIN      
        1 gagcgcaagg catacgtcac gcgcaccgac gtcgactact acaccgatgc cgacctcggc
       61 atcacgctca aggtgctcga ggtcttcgag gaggccgatg agcctcccgc cggcatgcga
      121 cagcgcggcg aggtcatggt cgcctggcag gtgaccatgt tcaagaagat caagtaccac
      181 acccacgaga acgtcggatg gggctcgatc agcatccccg agcaggagat gcacaccacc
      241 gcctgctggc tcgtcccgcc ggccgagctg gtgaatcgat acgaccgtga cacgctcgac
      301 ggggcgctca tcggcctggc gcgagtcgcc cgcacgaccg catcgctgct cctcatgtgc
      361 gacccgcgcg atctcggcgt gcaggcccag gtgcagtcgc ccttcaccgg ccggccgacg
      421 gtctacgtgt acgacgccgt accgggcggg gtgggcctga ccgagcgcct ctacacgctc
      481 accgacgagc tggtgcgagc atgccgggag gcggtcgact cgtgtgcctg caccgatggc
      541 tgcccggcct gcgtcggccc tgccatcgaa gttggctccc gcggcaaggc gaccgtctct
      601 gagctgctgg ccgggctctc ctgagcgatg cgcaccctcg ccgatcgact ggcggagatg
      661 cgggcagagc ggggaaagcc ggctccgccg cgcccggcgc ggctccccga caatcgcgcc
      721 gaagttctcg cgcgctggtt cggagcccgt ctacaggtcg cgcccgatgg cgcggcggtc
      781 gtcgtcgagc ggcgggtttc gctgccgccc gcgaccgtcg cgaacctggc tggcctgccc
      841 tcggcctgct acttcgacac ggagacgacc ggcctctcga ccggcgccgg aaccgtcgtc
      901 ttcctcgcgg gcctcggcca tcttgaaggc agccagctgg tcgtgcggca gctcctcctc
      961 cccgactatc cccacgaggc cgcgctgctc cggatcgcgt gcggcgagct ggcggcgctg
     1021 ccgcgagtcg tcacctacaa cggccgcggc ttcgacctgc cgctcctcat cacccgcctg
     1081 acagttcatc gcctgttccg cgagatgtcg gcattgcccg agctccatga cgacctgctg
     1141 ccggtcgcac gacgcctgta ccgccgcccg ctcggcggcg cgcggctcgc ggacgtggag
     1201 tccggcgtgc tcggggtgat ccgcacctcc gactgccctg gcagcgaggt cccgtcgcgc
     1261 tacttcggat acctgcgcgg cggctcgccg gacctcctga ccgacgtcct cgatcacaac
     1321 ctgcaggaca tcgtctcgct ggcgctgctg gaagccgaga tcgtccggat gcgcgccggc
     1381 ggctggcgag atgctccggt gctcgacccg cacgggatgg cggtcgagct gctacgccac
     1441 ggcgcgagcg acgaggcgct cgaggtggtc gaggcggcga tgctgcccgg ggtcgaccag
     1501 gatcgggcac attcactgcg gcggatggcg gcgcgcctgc tgatcagcgc cgggcaggtt
     1561 gaccgtgcgg aggagctgtg gactgccgcc acgcgccgcg cctcgaccga tgcagccgcg
     1621 gcctggctcg aggtggcgcg catccgcgaa cggcagcgtg gcgatctgca cggagcgctg
     1681 gaggccgctc tcacgagctc ccgcgtgctc gacctggcct ttgcccttgg ccggggaggc
     1741 gggatgctgg cggtcggtcg cgtgcggctg cgcgtcgatg cacggctgcg caggcttcgt
     1801 cgctgggtcg cggctgccga ccgtcgcgcg gccagggagc cccgcgtcgc ctgacgccgc
     1861 ctcgagcgtc taggcgcgcc gttcctccag gacttcggcg acggcagcac tgaaccgccc
     1921 ggagccgacg agccatcggc aaagcggtcg acggtcaggc cgccgatcac gagcagatcg
     1981 ggctgggcgg ctatcatcgg gccatcgtga aactcttctt ctacgagctg cacgaaggtg
     2041 caaccgatct gctgaccgat gccgtgctgg tcagcgacca ggagtacgcg ccggaggcgt
     2101 tcgcctcact cgtgcttgcc gccagggcgg ccgtggtcga caccttcgag gaggacacgc
     2161 tggtcgaggc gatcgcgcgc gagctggagc gcagccacgg cttcagctat gcgggggacg
     2221 agaagctgac ggccagcatg agcgtgggca tggacgaggc ggacacctac ctggtggaga
     2281 ccagcgacga gttccgcagc gtcatcgcgg aggtcgacct ctcctcgtag cggccgcctc
     2341 agcgcgcgac gttgaagagc acctcgatca cgtcgccgtc gcgcaccgcg tacgtcttcc
     2401 cctccgatcg gagcagcccg cgcttgcgtg cctcggccat gctgccgcag gcgatcaggt
     2461 cgtcgtaggt gaccacctcg gcgcggatga agccgcgcgc caggtcggta tggatcgccg
     2521 cggcggcgtc tactgccgtg gcgccgcggc gcagcgtcca ggcccggcac tcgtcctcgc
     2581 cggcagtgaa gaacgagaag agtccgagca gcgcgtaggt gaggtggatg acgcgaccgc
     2641 ggctcggctc cgcgatcccc aggtcctcca tgaagagcgc ggcatcggcc tcggagagct
     2701 gggtcagctc cgcttcgatc ttgccaccca gggcggcaac atcggtctgc ggctgcccgt
     2761 agcgggtgcg tccggcggcc tcgagctccg cggcggcccc aagctggccc tcgtcgacgt
     2821 tgaggaccac cagcgccggc ttctgggtca ggaaccgata gccacgcagg cgaagctcgt
     2881 cctcgtcgga cagcgcgaca gcgcgcagcg gctgcccggc tgacagctcc ggctccagtc
     2941 ggtcgaggag cgcctcctcc tgggcattcg cctctcgctc tgccggcgag ccatggcgcc
     3001 cggaagtccg cagcttctca aggcgcttct cgatcaccat caggtcggcg atcgcgaact
     3061 ccaggtcgag cgcctcgatg tcacgccatg ggtccggcgg accctccgcg ccgccgtcaa
     3121 aggcacgggc cacgtggagg agcgcatcgg cattgcggac cagggccagc acgtcggggt
     3181 tgatggtccc ctcgcgcgtc gcccctgccg gaatcgcaac gtcgacgtag gtgacgtcgg
     3241 ccggggtggt cttcttgggg tggaacagct cggcgaggcg atcgaggcgc tcgtcgggga
     3301 ccttgaccac gcccatgttc ggcgcggcgc gtccgccgga gaagcccccg gtgtcggcat
     3361 gggccccggt cagggcattg aagacggtcg tcttgccgct ccccggcagc ccgacgatgg
     3421 tgacctgcac cggctagtac ccggcctcca gatcgacgac gttctcgagc ggctcgcccg
     3481 cagcgaagcg gcgaaggtta gccacgaaca ggtccagcga gcggtccacc acacggtcgc
     3541 tcgaccacga ggtatgcggc gtgatgatca ggttcggggt gccatacagc ggcgaatcgg
     3601 gcgggagcgg ctcctcgttg aagacgtcca gcaccgcccc accgatccag cccgattcga
     3661 gcgcgcggcg cagcgcaagc tcgtcgagca ggcggccgcg tgcgatgttg atcagccacg
     3721 agtgctccgg catctcctgc agctgggcgg agccgatcag ccccgccgtg tcgtcggtca
     3781 gcggcgccgc cacgaccacg atgtcgctgc gtcgcaggag ctcgtcgagc tggtcgagcc
     3841 ccagcagctc gacgttcgac gcttcgccgg caccccggtc cggatgccgg cgggtcgcca
     3901 gcaccttcgc ctcgaagggg gcgagcagct tggcgatctc ggagccgatg ctgccgaagc
     3961 ccacgatgcc gaccgtcatg ccgcccagct cggtgccgcg cagcggctgc cacgtgcgct
     4021 cgcgctgcag ctccagcaac tgtggcaggc gccgagcgat ggccaggcac atcatcacca
     4081 cgtactccgc gatcggccgc gagaagacgc cgcgcgcgtt ggtcaccacc aggccgcgct
     4141 cgcgaaccgc gggagttgcc acgcggtcca ccccggccga gaaggagtgg atccattcca
     4201 gtcgcggggc gcgagcgatg atgtgatcga gcaccgaggc cggcacgccg cctcgcagca
     4261 gtacgcgcgc ggatgcgagc gccgcctccg catcgtcatg gaccagaccc tcgcgggata
     4321 tctgcacgac ccgcgcaccg ggcactgctt cgagctgcgc ggcgtgttcg gcggagagcg
     4381 gggcgccgaa gatcggcgtc gccaggatga cgatctcgtc ggtcagctga cctgctccct
     4441 ggccccgcgt ccctccatcc ggtcgatcca gccctgcggg tagcggatct cggcgaggtc
     4501 cggcaggttg gcgggacctt cccagacctg gttcaggaag gcgcggtccc gcaccgccag
     4561 gacggccttc acgaatcgct ccccggccgc gtactgctcc atcttcaggt cgaggccggt
     4621 gagacgcatg atcgcccgct ccagggcccc gcgcgcctcg ttgcggcgct cgaaccgctc
     4681 gtgcagcttc gtgaagcccg gcaggagccg ctcgccggcg tcgttcatga cgtggttgga
     4741 gtacccctcc agcagcgaca tcagcgcctg ggtgcgacgg aaggtgctca gctgggcggg
     4801 ggtcatcatc cgctccagcc agtgtccgtc gcggccgcgc agcgcgcccc gcagtcgctg
     4861 gccgagcccg ccggtgtcgg tcgccatctg ctcgatcgcc tcgccgaccg aagcggcgaa
     4921 atggtcacgc agccacgggt atgcctcgaa ctcgaaggcg tgggtcgcct cgtggagcgc
     4981 gacgaaggtg cggaactccc ccaacgaaac gccgagcgtg gccgcgctgg ccctgatgtt
     5041 cggctcgacg aagttcaacc gacctctcgc tgccggcgcc gcggcgagga ggctgacgtc
     5101 gtactggccg agcaccttgc gcgccaggaa cgccatcagc agccccagct gctggttgcc
     5161 gaacgaacgg ttcaggattc gtgcgagtgc gcggccggga gtatcggctc cggtggtggc
     5221 gtcgttgatg atccgctcga ctcggccaaa gagctgctcg aaggtcgcca ggttcaggtc
     5281 gatccactcg aagcgatcga ccactgccgg cgtctcgagc gcgcgcggca gctcggcgcc
     5341 gatcgcctcg gccacgatcg gctcgattcg cagcaactcg gcgcggtaga actcctccgc
     5401 tgccgaacgg tcggctgcgc tcagtgctgc cccctcgggt cccagccggc gccgcgcgat
     5461 gcgccgaacc gcttcccagt cgatgagacg cgtgccggct cggcggagca gctcgcgctg
     5521 cacggccgtg gcgcccatcg ccagcagtcc gccgacgacg atccccattc cgatacgact
     5581 tgcgctgcgt gccatgcggg gagtctagac ggctgccacc accgcctgct caaccgaggg
     5641 aagggcagcg ccgaagaccg tcgccgcaac ggcgctgaag gtccccacgt cgccggtggt
     5701 cacgatccgg ttgtcgcccg ggccgccgct cgccgttcga ccgccgaggg cgtcgagaag
     5761 gtcctctgtc gccagtgcgg tggtgaacgc ggaatccacc accgccactc cggggcctgt
     5821 cagccgctcg atcagcggcc gcaacagcgg atagtgggtg cagcccagca gcagcgtgtc
     5881 gatctcgcgg tccatcccga acagctcggt caggtatccg cccacggtga cctcggcaac
     5941 ggggccgtcc agctcacccg cctccaccat cggcaccagc tgcgggcagg ccagctgggt
     6001 cacgagcagt gcaggatcgg cctccccgat ggcggtggga taggcgcccg aggccaccgt
     6061 tcctgccgtg gcgatgaccc cgacgcgtcc gacgcgggtg gatgcggctg ccgcgagggc
     6121 gcccggccgc acgacgccca gcaccggcac cgccgccagc tcccggacca tggggagggc
     6181 ctgggcggtt gccgtgttgc aggcgaggac cagcagcttc acgtcctgcg acagcagcca
     6241 tgcgaccgac tccagggtga aggcgcgtac ctcgtccgcg ctgcggggac cgtatggagc
     6301 ccgagcgttg tcgcccaggt agagggtcga ctcggctggc agtcggcgac gcagctcggc
     6361 caggacggtc agcccaccga cgccggaatc gaagacgccg atcggccgag ggtcggtgcg
     6421 cagccccacc gcgacggtca gcggcgcgcg aggacgccgg cgcgcgccgg cagccgcgct
     6481 gcgatcgagg cggccatcgc ccggatcccc tgcgagacct gggcatcggg gttggccagc
     6541 acgaagggga caccctcgtt gttggcggcg atcaccagct tcccgtccga gacgacctcg
     6601 tgatcgatct ggcgattcag ggcctcctcg acatcggcct tgctgaagcc accggtcgaa
     6661 tccgaccggt tcaggactgc ctcgatcttg ctcggcgcat agccgatcgt ggcgaatgcc
     6721 tcatcggcca tggccagcgc gcggatcgcc atgctgtcat aggccagcac ctcgaggatc
     6781 aggtcggatg catcgaggaa ggccagcacg tcgtcggaca gggatgcgcg cgtgtcgacg
     6841 acgatcactt cgtagacctg gcggatgatc gagagcgcct tctcgatgtc gcgaacggtc
     6901 accatctcgg ccatctcgat ccgcggcggc gccagcagga cgtcgattcc ggacgggtcc
     6961 ttccacagca cctcggccag gtccgtgtcg cggatgcggt cggtcggcag gttcatgatc
     7021 gagggtgcag ccgcgggcgc tcgcagcagt ccgcgcatgt cgctgaattg aagcgaaccg
     7081 tcgatcaggc agacctgatg gtcggcgctc agcgccacgg ccaggttgaa ggccagggtc
     7141 gtccgcccca cgccgccctt gggcgagaag aggctgacca tgaccgaggc gttgtggccg
     7201 ccggcgacgt tgctctgctc gtggaccttg cggaccatgg tggtcaggtc gaacccggag
     7261 aacggcatct tgaggacagc gtcgatgccg gcggcagggt cctcggtgac tgggttctgt
     7321 ggcacggtca gcacgatcac cgacacggtg ggctcggtgc ggcggacctg ctcggcgacc
     7381 tgcatgccgg agacgcggcc ctgcagcagc gcgtcgacga tcaggacgtc cggtcgcagc
     7441 tcggtgaccg ccgagagcgc ctgctctccg gaggtgatca cgtcgagcat cttgatctgc
     7501 gactgggcct gcaggagcga ccgcatgtgc atcgccacct gcggaacgtc ctcgatcacc
     7561 agcagccgga tctgctcagc caaccttcac ctcgtgcgac ggagccgccg cgaattcgta
     7621 cgcgacgaca tagatccggc gcccgctctc ccgtcccagt ggctcgagat cgtgtgagcc
     7681 gtagctcaaa tcgaccacca tccccgcctg tttgacgagt gcggcaagcg cctcaggaga
     7741 tggataccag agctgatggc ttgccggaag ccgcgctatc gtaccatcgg gttgtactgc
     7801 atccgcgatc gtggtgaagg cgacgcggag cccctcgggc gactcgcggc gcaccaattc
     7861 gctgcgtcga accacctcac ggccatccat ctgacgccgc cagtcgatcg acagcggcat
     7921 gtcacggacg ggcatctcgg ctggacccag gtcgtcgata acgagccgcc cgcccttggc
     7981 caggaccgat cgggtgccgc ccagcaggcg cagcgcatcc tccgggccgt cgaggtgcgg
     8041 gagcacgccc acggccacca ccaggtcgaa tcgctcacga atgttgaggg tcgagatcgc
     8101 gcgcacgtca ccacgcatca ctgacagccg gccgtcccgg gccgcctggg cgagcccctc
     8161 gtccgcggcg atgcgcgcct cggcacggcg gagcagcgcg gccgaaccgt cgagcccgag
     8221 aatgcgagag gccccggcat ccagcagagt ccggaagaga cgcccggatc cgcagccaag
     8281 atcgaggacc ggtccgtcag tgcgcctggc aagttcgcgg tagaagggga gatcctcgac
     8341 cagctcgtcg tgctcgaggt cgtagagctc ggccagccgg tcctcgtccg ccggctgcga
     8401 cccgaagatg tcggcgctca cgccagcgcc ggacctaggg aaggtcgaag cggcgaagcg
     8461 cgatccattc cgtcgcggcg gccaggctga ccacgaacag cgcgaccggg accgacaggg
     8521 cgccgcccag gcgcaacccg gcgactgccg cgatcacggc ccccaccagc acgcctcgtc
     8581 gtatggcacg tccccaggcc ccgcgataga tgatccgccg ccgcaccgcg aattcggcga
     8641 gcagggcgaa cggcatgacc gccacgcccg ccgtcagcgc cgcgagggcg accactgcgg
     8701 cacgattgac cccggcattc ggacagggtt ggcccggcag gtctccggca cacgcgtttc
     8761 cggcgaaggc cacgagcacg accgccccga gcaccgccag cagcaccagc gcggcgactg
     8821 cgatgcgcga tcgggaggtc atcggatcgc gtcggggcag atctcacgga atggacagta
     8881 cccgcatctg gttggagacg ggttcgcgtc gaacctgccg gctcgga
//
LOCUS       DATCQR010000258         3357 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_169559, whole genome shotgun sequence.
ACCESSION   DATCQR010000258 DATCQR010000000
VERSION     DATCQR010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3357
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169559"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1949)
                     /locus_tag="VIN32_08445"
     CDS             complement(<1..1949)
                     /locus_tag="VIN32_08445"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HEY8922643.1"
                     /translation="MDARLRLEIRLLGRFSVRRAGQEIPAPELGGRLGRSLIRILLTR
                     RGTVLPVDVLAEALWPDRQPADPAANVAVLVSRVRRALKDPRLIAAATGGYLFTAGDC
                     CAVDAELFLDYAERGRAHLDLRRADAALKEFRAALESWAGEPLPEDTYSDWSREYRER
                     LLRSYVQVLEGGAEAALELGDVSLAETLAARAVAREPLRETGHLLLMRALAASGNQAA
                     ALATFDALKLVLAEELAVEPSREALELRQQVADGRLARGRLELRRSEPAVPAAHPAAA
                     LPFVGRREELAVIDTALSSLDRSVVVVTGPGGSGKSRLLSEAVARTRLPVVAARAFRP
                     EREEAWGTARSLFREALSLDPDAVRGIPDPAARALTDLLPEVAELRPLLDAAIDPESR
                     RALVLEGGARLVSAALRENGLIVIDDLEWADASSLELVEQVVRRRDRIHLILAYRPEE
                     VAEERAAAAFVAGLDDVTTVHRIALGPLPRLAIADLVEDEGLARTIAEETDGTPLAVS
                     EALRLLGSEGMIVPGPRGRWRTVVPGAGERGRAAVRAGQREAIRRRVDRLTPRCRTVL
                     RLLALLGREVPARLLARAIGSGQRQVLQALDLLSRIDLAAAGEGGWRVSHDVIGEAVV
                     EGLRQAELAQLHVLLAEALSLEAGDP"
     gene            complement(2391..2999)
                     /locus_tag="VIN32_08450"
     CDS             complement(2391..2999)
                     /locus_tag="VIN32_08450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8922644.1"
                     /translation="MVPNDARRHAREVAGLADELSVRLRVLEESGATLQVHVDARPIG
                     PGGGGLASLASTGIEVPADADIEALEFIVMMEAAKSAQDDLKAILAAVKSINVAKNRL
                     SSELEKARARGSSTSGKNSPRRGSADLDALLQVLLTAYGIGVEQEGERLINDLDSMSE
                     LGEIESLRLQMAMDRMSKMMSTLSNLLKKIADANQQIVQNLK"
BASE COUNT          566 a         1158 c         1127 g          506 t
ORIGIN      
        1 gggtcccccg cctccagcga cagcgcctcg gccaacagga cgtgcagctg cgccagctca
       61 gcctgtcgca atccctcgac gaccgcctcg ccgatcacgt cgtggctcac cctccagcct
      121 ccctccccgg ccgcggccag gtcgatccgg gagagcaggt ccagcgcctg gagtacctgc
      181 cgctggccgc tcccgatggc gcgggccagc agtctggcgg gtacttcccg accgaggagc
      241 gccagcaatc gaaggaccgt gcggcaacgt ggcgtaaggc gatcgacacg ccggcggatg
      301 gcctcgcgct gcccggcccg gaccgctgcc ctgcctcttt cgccggcccc gggtacgacg
      361 gtgcgccagc ggccacgcgg accgggcacg atcatcccct cgctacccag gaggcgcaac
      421 gcttccgaaa ccgccagggg cgtgccatcc gtctcctcgg caatcgttcg ggccagtccc
      481 tcgtcctcca cgaggtcggc gatcgccagc cgcggtagcg gcccgagcgc gatcctgtgg
      541 accgttgtca catcgtcgag cccggcgacg aaggccgcgg ccgcccgctc ttcagcaacc
      601 tcctcggggc ggtaggcaag aatgagatgg atgcggtcgc gccggcggac gacctgttcg
      661 acgagttcca agctgctggc gtcggcccac tccaggtcat cgatgacgat cagcccgttc
      721 tcgcggagcg cggctgacac cagtcgggcg cccccttcga ggaccaaggc gcgccggctc
      781 tcaggatcga tggcggcgtc gaggaggggg cgcaactcgg cgacctcggg cagcaggtcc
      841 gtgagggcgc gggcggccgg atcgggaatg cctcgtacgg catcggggtc gagcgagagc
      901 gcctcccgga agagcgaccg cgccgtaccc caggcctcct cacgctcggg gcggaaggcg
      961 cgggcggcca cgactggcag acgcgtgcgt gccacagcct cggaaaggag cctggacttt
     1021 cccgagcccc cgggtcccgt cacaacgacg actgagcggt cgaggctcga tagcgcggta
     1081 tcaatgacag cgagctcttc ccgccgaccg acgaaaggaa gcgccgccgc cggatgcgca
     1141 gccggcaccg ctggctccga tcgacggagc tcgagtcggc cgcgggcaag gcggccgtcg
     1201 gcgacctgct gccggagctc caaggcctcg cgtgacggct ccacggcgag ctcctcggcc
     1261 agcaccaact tgagggcgtc gaacgtcgca agcgccgctg cctggttgcc tgatgcggcg
     1321 agggcccgca tgaggaggag gtgtcccgtt tcccggagcg gctcgcgggc gactgcccgc
     1381 gcggcaagag tctcagccag cgacacgtcg ccaagctcca gcgccgcttc cgctccgcct
     1441 tcgagcacct ggacatacga acgcagcagt cgctccctgt actcccggga ccaatcggag
     1501 tacgtgtcct ccggaagcgg ttccccagcc cacgactcga gagccgcccg gaactccttg
     1561 agcgcggcat ccgcgcgtcg taaatcgagg tgggcgcggc cgcgctccgc gtaatccagg
     1621 aaaagctcgg cgtccacggc gcagcagtcg ccggcggtga ataggtagcc ccccgtcgcc
     1681 gcagcaatga gtctgggatc cttgagcgcc cgccgaacgc ggctgacaag gaccgccaca
     1741 ttggccgccg ggtccgcagg ttggcgatcc ggccacaggg cctcagccag gacgtcgacc
     1801 ggcagcaccg ttccgcgccg cgtcagcagg attcggatca ggctgcgccc caggcgccca
     1861 ccgagttcgg gcgcggggat ctcctgtccg gcgcgtcgta cggagaaacg tcccaggaga
     1921 cggatttcca gccggagcct ggcgtccatc gtcccaatta cctccgggcc gcaatgaaat
     1981 tgcaaacagg tcctgccagc gtcggcgcga cgccaagagc gcgctgaagc cacttcagga
     2041 gcaggccaac gagggtgaga tggcggagta cctgctgcac cacgtgcacc ggcccgaaca
     2101 gtgtcaggag atttccgagg cctggagagc gcccgacgca ccgaccaaaa tgaggggaca
     2161 cgagttcttt agctactgcc ggagcggcga tcacggcgcc ttcatcggtg cggaagcaga
     2221 cagcccggag actgccctgg cgttactacc ggctctcctg cgaccgacga cgcgagtgta
     2281 cgcaggggag acgatgagga tcgagaccgg gattgctgag gtcgtgaacc gaacgcgtcg
     2341 ccgtggctga gcgcacgatc aggtcatcgt gccggtcgcg cagaagcgcg ctatttgagg
     2401 ttctgaacga tttgctgatt ggcgtcggcg atcttcttca gaaggttaga gagcgtgctc
     2461 atcatcttgc tcatgcggtc catcgccatc tgaaggcgga ggctctcaat ttcacccagt
     2521 tcgctcatcg agtcgaggtc attgatcaga cgctcgccct cttgttcgac gcctatgccg
     2581 taggcggtga gcaagacctg aaggagagcg tccagatccg cactcccgcg tcgcggactg
     2641 ttcttgccgc tagtcgacga gcctcgggcg cgggccttct cgagctcgct actaaggcgg
     2701 ttcttggcca cgttgatcga tttgacggcc gcgaggatcg ctttgaggtc gtcctgcgct
     2761 gacttcgcgg cctccatcat cacgatgaac tcgagggcct cgatgtccgc gtctgcgggg
     2821 acctcgatgc ctgtactcgc gagcgaggcg aggccgcctc ctccaggtcc gatcgggcgc
     2881 gcgtctacgt gtacctgcag cgtcgcgccc gattcctcga gcacccgcag gcggacagaa
     2941 agttcatcgg ccagcccggc gacctcgcgt gcgtgccgac gtgcgtcgtt cggaaccatg
     3001 tagtcaaccc tcagtgcgat cgtcgtcggc gaggaaggat tcggaccgta ttccgccgta
     3061 agcagcgtcg cccctgcgcc gaaggctcta ggaaatcagg ggcagacacg ggcgtcccga
     3121 atctaccgcg accgccaact cggctgcagg tcgccgaggg gtccaagcgg cagtaaggct
     3181 cggcgcacga tccggccatc gtgcggggcg caatgcaccg cccacggcca ccaaggcgag
     3241 tcagggtgcc acctggggcg gcttcgaaga gccgcccgat ggtggcgtcc tacggacccg
     3301 cgccaggttg gtcagcgact cgcgagcgtc ggcagctggc cgagctgggt catgaag
//
LOCUS       DATCQR010000259         2305 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_199792, whole genome shotgun sequence.
ACCESSION   DATCQR010000259 DATCQR010000000
VERSION     DATCQR010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2305
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199792"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(835..1704)
                     /locus_tag="VIN32_08455"
     CDS             complement(835..1704)
                     /locus_tag="VIN32_08455"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEY8922645.1"
                     /translation="MEVLKITPRGYCHGVVDAFRIAKRVREETTGPVHMLGMLVHNTH
                     ATDDLQRQGVELVDQNDRLAGLSQIKGGTVIFTAHGVSPQVKQRAVELGLTPIDATCS
                     DVVRTHELVADLARKGYEVVYIGRKGHPEPEGVIGEAPGKVHLVQDPEDIDALELKGD
                     RIAVTCQTTLSVWDTEDLIGRVQARYPNAEVHNEICRATQERQEAAVEAAPLVDLVIV
                     VGSPRSSNSLRLVEVVKKLGHKPAYLVDNLEDLDLAWFNGAKKVGVTSGASTPSQLTR
                     RVIEYLEALEVPA"
     gene            complement(1739..>2305)
                     /locus_tag="VIN32_08460"
     CDS             complement(1739..>2305)
                     /locus_tag="VIN32_08460"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEY8922646.1"
                     /translation="DSGMVGTPENDDPRSFHQAPLHEAAAKLAAILREERPDVVVTYD
                     ADGTYGHPDHIKAHLVTNAALDLLEKEGWRPSKLYYTGIPRTLMQQFMESLPEDARRQ
                     EQEGTIRIPGTPDELITTQLDVGEYVDRKREAFAAHVTQNDPNSWFTTMADQIYRLAF
                     GTEYYRLARGKPGSKLPENDLFVGVADK"
BASE COUNT          351 a          791 c          761 g          402 t
ORIGIN      
        1 ttgttcgggc tatactcggc ccgacctgga gccgatctct ttcgacgacc tccccctcgg
       61 cggcgagtgg acgacccggc ggcgcaccat ctccgaatct gagatcgcgc tgttcgccgc
      121 cgttgccggc gacttctccc ccctcacgat cgatgccacc caccgaaatg gacgcatcgc
      181 ccctcctgcc ctcctcgtcg ccatggccgt cggcctgggc tccgtggaca tgccggtgcc
      241 ggccgtcgcc gagtgggagt ggctcaactg gaagtttccc cgcgccgtcc gcgcaggaga
      301 cacgatctac gctcgctgga cgctgaccca gaaacgcgcc ccggtcgggg gttcgcccac
      361 agcaatagtg gtctggagag tggacgtcca tacggcaggc ggcgcgctct gcgccgaggg
      421 cgaggtcgga gccagcgtgc tgcgcgcttc ccaggcccga accgccgagt ccgccgcaag
      481 agagccggtc gccgaaccgg cgacaactgc agcgtctggc gtgatcccac ggagggggcg
      541 acggcgacga cgcccatcgc ccggtgacgc gaccaagacg aaggcggtag acacagtgga
      601 ggctcccgtc caggccccgt ccataggggc ggcaactgct gggccggagc gaacggtaag
      661 cggcaggcgc cggcgccggc gccggcctgc gagctccact gcggcccgaa acggcgctac
      721 caccgctccg acagctccga cagacgcccc aaccgcgacg gctgcagtgc cagaggcgac
      781 cgcacccgac accgcaaacc cgctgaggcg cgcgatcagg cgattgcgtc gaccctaggc
      841 cgggacctcc agagcctcca ggtattcgat cacccgacgg gtgagctggc ttggggtgga
      901 ggccccgctc gtgacgccaa ccttcttggc tccgttgaac caggcgagat ccaggtcctc
      961 caggttgtcg acgaggtagg ccggcttgtg gccaagcttc ttgacgacct cgaccagcct
     1021 gagcgagttc gatgatcgtg ggctacccac cacgatcacc aggtcgacga gtggcgccgc
     1081 ctcgacggcg gcttcctgcc gctcctgcgt cgcgcggcag atctcgttgt gtacctcggc
     1141 gttcgggtag cgggcctgga cgcggccgat caggtcttcg gtatcccaca cggacagggt
     1201 ggtctggcag gtgaccgcga tccgatctcc tttcagctca agggcgtcga tgtcctcggg
     1261 gtcctggacg aggtgcacct tgcccggagc ctcccctatc acgccttccg gctcgggatg
     1321 ccccttccga ccgatgtaga cgacctcgta gcccttccgg gcgagatcgg ccaccagctc
     1381 gtgcgtgcgg acgacgtcag agcaggttgc gtcgatcggg gtcaagccaa gctccaccgc
     1441 ccgctgcttg acctggggcg agacgccgtg ggcggtgaaa atcacggtgc cgcccttgat
     1501 ctggctaagg ccggccagcc ggtcgttctg gtccaccagc tcgaccccct ggcgctgcag
     1561 atcgtcggtg gcgtgggtgt tgtgaaccag catcccgagc atgtgcacgg gcccagtggt
     1621 ctcctcacgc acgcgcttgg caatgcgaaa ggcatcgact acgccgtggc agtagccccg
     1681 cggggtgatc ttcaggactt ccatctcttt tgagtctacg cggaggggtt gagccggctt
     1741 acttgtcggc cacacccacg aagaggtcgt tctccggcag ctttgagccc ggcttgccgc
     1801 gcgccagccg gtaatactcg gtgccgaacg ccaggcggta gatctggtcc gccatcgtcg
     1861 tgaaccatga gttggggtcg ttctgggtca cgtgcgcggc gaaggcctca cgcttgcggt
     1921 cgacatactc gccgacgtcg agctgcgtcg tgatcagctc gtccggtgtc cccggaatcc
     1981 tgatggtgcc ctcctgctcc tgccgtcgcg cgtcctcagg cagcgattcc atgaactgct
     2041 gcatcagcgt gcgagggatg cccgtgtagt agagcttgct ggggcgccac ccctcctttt
     2101 cgaggaggtc gagcgcggcg ttggtgacga ggtgcgcctt gatgtggtca gggtgcccgt
     2161 aggtgccatc cgcgtcgtaa gtcacgacga cgtccggccg ctcctcccgc aggattgctg
     2221 ccagcttggc ggccgcttcg tgcaacggcg cctggtgaaa ggacctcggg tcatcgttct
     2281 cgggcgttcc gaccatgccc gagtc
//
LOCUS       DATCQR010000260         4557 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_216202, whole genome shotgun sequence.
ACCESSION   DATCQR010000260 DATCQR010000000
VERSION     DATCQR010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4557
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216202"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="VIN32_08465"
     CDS             complement(<1..398)
                     /locus_tag="VIN32_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020001167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HEY8922647.1"
                     /translation="MPTTSNVSAKLIFPTKEIVEEYYLPIIYTACRTCYSEQLPDQIW
                     DKAVSRGVADEKQQALVRKVMESGHGSTIEHVNFTFAISGVTRTLSHQLVRHRAGTAF
                     DQQSQRYVSFKQRDNYTIPDSIAKGDLPDDL"
     gene            complement(481..708)
                     /locus_tag="VIN32_08470"
     CDS             complement(481..708)
                     /locus_tag="VIN32_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8922648.1"
                     /translation="MTKARWLGIGLIVAGLAVLLVAPQAQSCVTRFDGTACQASGTVL
                     LKVVGLGLVAAAAAVLARHGNPAGGSGDSTR"
     gene            complement(705..2099)
                     /locus_tag="VIN32_08475"
     CDS             complement(705..2099)
                     /locus_tag="VIN32_08475"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEY8922649.1"
                     /translation="MAVLLPLAALVLLRAFPSLDAPTLPSVALAVAASVALIAGGALL
                     AGVAAALERGRIRDLADLAGLGLIATSFAVVAFDSAGTLGLAIGVTAAAAAFNVGAVM
                     RRWAVTTGRGRVVGIVIALALIEAGLATILTAAGWSIPEGTATLFLGGGALLMAAAAT
                     GSLDDPARATALGIAASSAGAIAIAAPSGTESLVGPAGMGLAALVLGSRFVADRRQRP
                     AVAEPAAALSELPGPLDTPPEYDELARLTRELRATLDDLVAARHLIELQRIEIDRATS
                     TDALTGLPSRWPTLDRLRTEAAEGRRYAHPVAAVLLDIDHFAQLNHDQGLEVGDEMLR
                     RIALRLRVRMREADAVGRVGADAFLAILPHTDEEGAATFARAVLDRVLERQVMTERGE
                     VTVSLSIGIALMRPGMTLTGDELLAAAEEALASARAAGGNRIAFDRLHGLARLDEPEG
                     APQSSVHDVAEGTA"
     gene            2255..2620
                     /locus_tag="VIN32_08480"
     CDS             2255..2620
                     /locus_tag="VIN32_08480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8922650.1"
                     /translation="MASPTSRRTAMSRRRFLEQTDQLIADGDALVATPHWDLFRAWLL
                     NSDELLERVWGRMDRYHLAWLNVGRDSAPSGSDLDEAGTARFITEVASAKVAVLRTMR
                     VAVATRGWRNLSDEDEEDR"
     gene            2617..3345
                     /locus_tag="VIN32_08485"
     CDS             2617..3345
                     /locus_tag="VIN32_08485"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HEY8922651.1"
                     /translation="MTDRQREPIGGLAAVFAHPDDESFTAAGALALAHDAGRTVRLLL
                     LTRGEAGNPERSPDLDLADTREEEMRCAAAKIGMDEVTVVDHPDGRLSEVPFSTLVDE
                     IALWLADRRPDAVITFGAHGVTNDADHVVVGAATRWAVERLAESGIAPHAVYVVAPVF
                     GPGQRRYDLSPEEGAASHRIEITDVAGRKLAALACHVSQTDVTEEIAELRAALDRDGV
                     VYEGYTRVRPTVPAARPTFYSRLV"
     gene            3360..3686
                     /locus_tag="VIN32_08490"
     CDS             3360..3686
                     /locus_tag="VIN32_08490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8922652.1"
                     /translation="MEQAPRARRPSAAWTILAGLAMVATLLVDGVLLWGVVSYPLAGR
                     PLTPGDWIVAAVLAGAMGGALVVLLRLIGRRRPGWLGLLVAALGLALPGGIALQIALS
                     GPPFIS"
     gene            3796..4086
                     /locus_tag="VIN32_08495"
     CDS             3796..4086
                     /locus_tag="VIN32_08495"
                     /inference="COORDINATES: protein motif:HMM:NF013999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HEY8922653.1"
                     /translation="MTIDSNLAKVDEVMTALYDIHDPEIGMSIVDLDLIKNVEIGVDG
                     APTEIKMILTTPFCPWAGELIQTVKQKTEEVVGPPVKVTLLADRWEPPPGLF"
     gene            4323..>4557
                     /locus_tag="VIN32_08500"
     CDS             4323..>4557
                     /locus_tag="VIN32_08500"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF014033.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009235 - cobalamin metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP:cob(I)alamin adenosyltransferase"
                     /protein_id="HEY8922654.1"
                     /translation="MRIYTRKGDTGTTGLLFGGARVSKADLRTDAYGTTDEAVSALGL
                     ARAAIGAVTDRTEAQLEPLVERLQRELFVVGAEL"
BASE COUNT          693 a         1594 c         1587 g          683 t
ORIGIN      
        1 aggtcgtccg gcaggtcgcc cttggcgatc gagtccggaa ttgtgtagtt gtcgcgctgc
       61 ttgaagctga cgtagcgctg cgactgctgg tcgaaggcgg tcccggcccg atggcgcacc
      121 agctgatgcg agagcgttcg ggtcacgccg ctgatggcga aggtgaagtt gacgtgctcg
      181 atggtcgagc catgccccga ttccatcacc ttgcgcacga gggcctgctg cttctcgtcc
      241 gccaccccgc gagacaccgc cttatcccag atctggtcgg ggagctgctc ggagtagcag
      301 gtccggcacg cggtgtagat gatcgggagg tagtactcct cgacgatctc cttggtcgga
      361 aagatcagct tggcggagac gttagaggtg gtcggcatgg gacggcgagg ataccaccgc
      421 cggggccgat cggggggcgc ttatccacaa cctgtagcgg tgtggacaac tggcgacccg
      481 ctatcttgtg ctgtcgcccg atccgccggc ggggttgcca tgcctcgcca gaacggctgc
      541 cgcggcggcc accaggccaa ggccaacgac cttgaggagg accgtgccgc tcgcctggca
      601 ggcggtgccg tcgaaccgcg tgacgcagga ctgcgcctgc ggtgcgacca gcagcacggc
      661 aaggccggcc acgatcaggc cgatccccag ccagcgcgcc ttggtcatgc ggtcccctcg
      721 gccacgtcgt gcacggagct ctgtggggct ccctcaggct cgtcgaggcg agccagccca
      781 tggagccgat cgaaagcgat ccggttgccg ccggccgcgc gcgcggacgc cagcgcctcc
      841 tcggctgcgg ccaggagctc gtcgcccgtg agcgtcatgc cgggacgcat cagcgcgatc
      901 ccgatcgaga ggctgacagt gacctcgccc cgttcggtca tgacctggcg ctcgagcacg
      961 cgatccagca cggcccgggc gaaggtggcg gcgccctctt cgtcggtgtg gggcaggatg
     1021 gccaggaagg catcggcgcc gacgcgaccc accgcatcgg cctcgcgcat ccgtacccgc
     1081 agccgaagcg caatcctgcg cagcatctcg tcgccaacct ccagaccctg gtcgtggttg
     1141 agttgggcga agtggtcgat gtccagcagg acggctgcca ccgggtgggc atagcgcctt
     1201 ccctcggccg cctcggtccg caatcggtcg agggttggcc agcggctggg caggccggtc
     1261 agcgcatcgg tgctggtggc ccggtcgatc tcgatccgct gcagctcgat caggtgacgg
     1321 gcggcgacca ggtcatcgag cgtggcgcgc agctcgcggg tgagtcgggc gagctcgtcg
     1381 tactcgggcg gcgtgtcgag agggcctgga agctcggaaa gggccgcagc cggttcggct
     1441 actgccggcc gttgccggcg atcggccaca aatcgcgagc cgagcacgag agcggccagg
     1501 cccatgccgg ccggacccac caggctttcg gtgccggacg gagccgcgat cgcgatggcg
     1561 cccgcggatg acgccgcgat gccgagcgcg gtggctcggg ctgggtcgtc caggctgcct
     1621 gtcgcggccg ccgccatcaa cagcgctccg cctcccagga agagtgtcgc ggtgccttca
     1681 gggatcgacc agcctgccgc ggtgagaatt gtcgccaggc cggcctcgat cagcgctaat
     1741 gcaatgacga tgccaacgac gcgtccgcgc ccggtggtca cggcccatcg ccgcattacc
     1801 gccccgacgt tgaaggcggc ggccgccgcg gtcaccccga tcgcaaggcc aagcgttccg
     1861 gctgaatcga aggcgacgac ggcgaacgag gtcgcgatga gccccaggcc ggcaagatcg
     1921 gccagatcgc gaattcggcc ccgttccagg gcagccgcca ctcctgccaa gagcgcgccg
     1981 ccggcgatga gagccacgct cgcggctacg gccagggcga ccgaagggag cgtcggtgca
     2041 tccagggagg ggaacgctcg cagcagcacg agggcagcga gcggcaggag gaccgccacg
     2101 ttgcggcctc cagtgcgcgc gagggtcgcg ccagatgatc ggttcaaggg catctcgcgg
     2161 tgcggccgtg caccgccgag tgtccgaggc ccgcctcccc ggcggcaata gtacgaacgg
     2221 ggcgcccgtg ctccggctgc cgtatcctcg gaccatggcc agcccgacct cccgccgcac
     2281 tgcgatgtcc cgccgcaggt tcctcgaaca gaccgatcag ctgatcgccg acggcgacgc
     2341 gctggtcgcc actccccact gggatctctt ccgtgcctgg ctgctcaact ccgacgagct
     2401 gctcgaacgc gtgtggggcc ggatggaccg ataccacctg gcctggctca acgtcggccg
     2461 cgactcggct ccatctggct ccgacctgga cgaggcgggc acggctcgct tcatcaccga
     2521 ggtggccagc gccaaggttg ccgtgctgcg taccatgcgg gtcgccgttg cgacgcgcgg
     2581 ctggcgaaac ctgagcgacg aagacgagga ggatcggtga ccgatcgtca gcgtgagccg
     2641 atcggcggcc tggcggccgt cttcgcgcat cccgacgacg agagcttcac cgcagccggc
     2701 gccctcgcgc tcgctcacga cgcgggccgg acggtgcggc tgctgctcct cacccgcggt
     2761 gaggccggca accccgagcg gtcaccggac ctcgacctgg ccgatacccg cgaggaggag
     2821 atgcgctgtg cagccgccaa gatcgggatg gacgaggtca ccgtcgtcga ccatccggac
     2881 ggccggctgt ccgaggttcc gttctcgacc ctcgtcgacg agatcgccct gtggctggcc
     2941 gaccggcggc cggacgcggt catcaccttc ggcgcccacg gcgtcaccaa cgatgcggat
     3001 cacgtggtgg tcggcgccgc gacccgttgg gccgtggagc gcctggccga atccgggatc
     3061 gccccgcacg cggtgtacgt ggtagccccg gtcttcggac ctggacagcg tcgctatgac
     3121 ctctccccgg aggagggggc ggccagccac cggatcgaga tcaccgacgt cgccggccgc
     3181 aagctggccg ccctggcttg ccacgtcagc cagaccgatg tcaccgagga gatcgcggag
     3241 ctgcgcgccg cgctcgaccg cgacggggtg gtgtacgagg gctacacccg agtccgcccc
     3301 accgtcccgg cggcccgccc gaccttctac tcgcgcctgg tgtagcgcgc cggcgtggca
     3361 tggagcaggc cccacgcgca cgccggccat ccgccgcctg gacgatcctg gccgggctgg
     3421 cgatggtggc cacgctcctg gtcgatggag tgctgctgtg gggcgtcgtt tcctatccgc
     3481 tcgccggccg ccccctgaca cctggtgatt ggatcgtcgc agccgtactg gcgggcgcca
     3541 tgggcggcgc gctcgtggtc cttctccgac tcatcgggcg gcgccgaccc ggctggctgg
     3601 ggctcctggt cgcggcgctc ggcctggcgc tgccgggcgg gatcgcactc cagattgccc
     3661 tgtcgggtcc acccttcatc tcctgagctg ccggcacttc ccgcgcttga tgcgcggggc
     3721 ttcgagcgtg gtgcagggcg ccggacgggt ggcccccgga agctcccgtg cgaatccttg
     3781 ctagactggt cggcaatgac cattgactcg aacctcgcca aggtcgacga ggtgatgacg
     3841 gccctctacg acatccacga cccggagatc gggatgagca tcgtggacct ggacctgatc
     3901 aagaacgtcg agatcggggt cgacggcgcc ccgaccgaga tcaagatgat cctcaccacc
     3961 ccgttctgcc cgtgggccgg ggagctgatc cagacggtca agcagaagac cgaggaggtc
     4021 gtcgggcctc ccgtcaaggt cacgctgctg gccgatcgct gggagccccc gcccggcctc
     4081 ttctaggccc cgatcacgcc tggatgttcg cgcgtaccaa ctccattggt acaagcccgc
     4141 atctggacgc aaatccaggc ccatccggcc ggaaacacat gtccccacca gtgacattgg
     4201 tagcggccca cgaagctgat ccctgtggtg ctgttctacc aatggggttg gtagacggcc
     4261 gccgtctcgg cgcaccatga gtaacaaccc cgaaagcgcg cagcggcgct agcatctccc
     4321 cgatgcgcat ctacacccgc aagggcgaca cgggcaccac cgggctcctt ttcggcggcg
     4381 cccgcgtgag caaggcggac ctgcgcacgg acgcgtacgg caccaccgat gaagcggtct
     4441 ccgcactggg gctcgcgcgg gcagccatcg gcgccgtcac cgaccgcacc gaggcccagc
     4501 tggagcccct cgtcgagcgg ttgcagcgcg agctcttcgt ggtcggggcc gagctgg
//
LOCUS       DATCQR010000261         3489 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_15031, whole genome shotgun sequence.
ACCESSION   DATCQR010000261 DATCQR010000000
VERSION     DATCQR010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3489
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15031"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2565)
                     /gene="carB"
                     /locus_tag="VIN32_08505"
     CDS             complement(<1..2565)
                     /gene="carB"
                     /locus_tag="VIN32_08505"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEY8922655.1"
                     /translation="MTPRKVLVIGSGPIVIGQAAEFDYAGVQACLALRGEGVETILVN
                     SNPATVMTDPDVGGTVVIGPLTVEHLERVIAEHRPDALLPTLGGQTGLNLAVALDDAG
                     VLERYEVRVLGTPLAAVRAAEDRGGFRDLVTRIGEPVPESAVVETIEEGLRFVEELGA
                     PVVVRPAFTLGGGGGGFASNLEEARIRLATGLAASPIGQVLVERSLLGWYEIEFEVLR
                     DASDTAIAICGMENLDPMGVHTGDSIVVAPIQTLPDQVVQRLRRCALKIVRELRLEGG
                     CNVQLAVRPDGGDYRVIEVNPRVSRSSALASKATGYPIARVAALIALGKRLHELPNPA
                     TGSGSAAFEPAVDYVVVKLPRWPFDKFPTADRSLGIQMKATGEAMAIDREFGTALLKA
                     IRSLEPRGHGWLWEDPTWDLSHRPPDDLASFLEPTDTRLWRMVSLLRHGWTDAAGLSQ
                     ATGIAPWFSERLAELVEAEQALVGMPLLEAKQLGFGDADIATLSGVPAVAVRRARVRA
                     GIRPAYRRVDTCAGEFPAETPYFYSSHTVAEVAPPANRRSVIVVGSGPIRIGQGIEFD
                     YCSVRAAWAIREMGLDAVVINNNPETVSTDYDACSRLYFEPLDAESVLDVVDHERALT
                     GEPPHVVLTFGGQTAIDLAKELAYADVPIAGLTAQAIELTEDRERFAALLDELGIEGP
                     RGKLAGNAAELSSVVGELGLPVIVRPSWVIGGRGIAVLESERDVVALLMTEVGWPLRV
                     DPMVDGVELDVDVISDGQDWCVPGILEQLDPPGVHSGDSVAVLPPQRIPRQLQEAAAE
                     TAGRIALRLGVRGILNVQMIVADQRIVVIEANPRASRTVPIVAKATGHDIVAAAVRC"
     gene            complement(2562..>3489)
                     /locus_tag="VIN32_08510"
     CDS             complement(2562..>3489)
                     /locus_tag="VIN32_08510"
                     /inference="COORDINATES: protein motif:HMM:NF009475.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HEY8922656.1"
                     /translation="HPMVGNYRIDPNELESDRVHPRAVIVTRLVTPPAGPGRTFEELL
                     IEAGIPALEGIDTRALTLELRRGARRKAVIRAAAEESGAVEQASQIIGWEAVDHVAAV
                     SARTPYSLSPVGALRGRATLVDFGVKRSLLHALTGRGLQVEVLPHDATAVDVAATQPD
                     LVVLSPGPGDPSRMAPQIETVRQLAQRAVAGGPPVLGICLGHQLLALAAGAGTRRLAV
                     GHHGGNHAVLELATGRVDIGAHNHEVEVLDGPMLAAAGYRVSHQDLNDGTVEGLVHAS
                     GRIASVQFHPEGAPGPIDAARVFDLAVSDVTA"
BASE COUNT          534 a         1285 c         1167 g          503 t
ORIGIN      
        1 gcatcgcacc gcggcggcaa caatgtcatg tccggtcgcc ttggccacga tcggcaccgt
       61 ccggctcgca cgaggattgg cttcgatcac gacgatccgc tgatcggcaa cgatcatctg
      121 gacgttcagg attccccgca ccccaaggcg cagggcgatg cgcccggccg tttcggcggc
      181 tgcctcctgc aattgacgtg gaatgcgctg cgggggcagg accgcgacgg aatcgcccga
      241 gtgcacaccg ggagggtcaa gctgctcgag gatcccgggc acgcaccagt cctgcccatc
      301 gctgatgacg tccacgtcga gctcgactcc atcgaccatc gggtccactc gcagcggcca
      361 gccgacctcg gtcatgagca gggcgacgac gtcacgctcg ctctccagga ccgcgatccc
      421 ccgcccgccg atcacccacg acggccggac gatgacgggc agcccgagtt cgccgaccac
      481 ggagctcagc tccgccgcgt ttcctgccag cttcccgcgc ggcccctcga tgccgagctc
      541 gtcgaggagc gcggcgaagc gctcccggtc ctcggtcagc tcgatcgcct gggcggtcag
      601 cccggcgatc ggcacatccg cataagccag ctccttggcc aggtcgatcg ccgtctgccc
      661 cccgaaggtg aggacgacgt ggggcggctc gccggtgagc gcacgctcat ggtcgaccac
      721 gtcgagcacg ctctcggcgt cgagcggctc gaagtagagg cgcgagcagg cgtcgtagtc
      781 ggtcgacacc gtctccgggt tgttgttgat gacgaccgcg tcaaggccca tctcgcggat
      841 cgcccacgcg gctcgcaccg agcagtagtc gaactcgatc ccctgcccga tgcggatcgg
      901 tccagagccg accacgatca cgcttcgccg attcgccggc ggggcaacct cggcaacggt
      961 gtgcgaggag tagaaatagg gagtctctgc cgggaactcg ccggcgcagg tgtccacccg
     1021 ccgatacgcg gggcgaatcc ccgcgcgaac gcgcgcgcgt cgcacggcca ccgccgggac
     1081 gccggagaga gtcgcgatgt cggcgtcgcc gaagcccagc tgcttggcct cgagcagcgg
     1141 catgccgacc agcgcctgct cggcctccac gagctccgcc agccgttcgc tgaaccaggg
     1201 cgcgatccca gtggcctggc tcagcccggc ggcatcggtc cagccatggc gcagcaggct
     1261 caccatccgc cacaggcggg tgtcggtggg ttccaggaat gaggcaaggt catccggagg
     1321 ccgatgcgag aggtcccagg tggggtcctc ccacagccag ccgtgcccgc gcggctccag
     1381 ggagcggatc gccttgagga gtgccgtgcc gaactcacgg tcgatcgcca tcgcctcgcc
     1441 ggtcgccttc atctggatcc ccagtgaccg atccgccgtc gggaacttgt cgaagggcca
     1501 gcgcggcagc ttgacgacca cgtagtcgac cgcaggctcg aatgcggcgc tgccagaccc
     1561 ggtagcgggg ttcggcagct cgtgcagccg cttgccgagg gcgatgaggg cagcaacccg
     1621 cgcgatcggg tagccggtcg ccttgcttgc cagcgccgat gagcggctga cccgcgggtt
     1681 gacctcgatc acccggtagt ccccgccgtc gggccgcacc gccagctgca cgttgcagcc
     1741 gccttccagc cgcagctcgc gcacgatctt cagcgcgcac cgtcgcagcc gctggacgac
     1801 ctggtccggc agggtctgga tgggagccac cacgatcgaa tccccggtgt gcacgcccat
     1861 cgggtcgagg ttctccatgc cgcagatcgc gatcgccgtg tcggacgcgt cgcgcagcac
     1921 ctcgaactcg atctcgtacc agccgagcag gctgcgctcg accagcacct ggccgatggg
     1981 gctggctgcc agtcctgtcg ccaggcgtat ccgtgcctcc tcgagattcg acgcgaaccc
     2041 gcctccgccg cctccgaggg tgaatgccgg ccgcacgacc accggggcgc ccagctcttc
     2101 cacgaaacgg aggccctcct cgatcgtctc gaccacggcg ctctcgggca ccggctcccc
     2161 gatccgcgtg accagatccc ggaacccgcc gcgatcctcc gccgcgcgga ccgcggccag
     2221 gggcgtgccc agcactcgca cctcgtaccg ctccaggacg ccggcatcgt cgagcgcaac
     2281 cgccaggttc aggcccgtct gcccgccgag ggtcggcagg agggcgtcgg ggcggtgctc
     2341 ggcgatgacc cgctccagat gctcgaccgt caatggcccg atcaccaccg tgccgcccac
     2401 gtcgggatcg gtcatgaccg tggcgggatt cgagttgacc aggatcgtct cgacaccctc
     2461 gccgcgcagc gccaggcagg cctgcacccc ggcgtagtcg aactccgcgg cctgtccgat
     2521 gacgatcggt ccggagccga tgacgagcac cttgcgtggc gtcatgccgt cacatcgctg
     2581 accgccaggt cgaagacgcg cgccgcgtca atcggtccgg gagccccttc gggatgaaat
     2641 tggaccgatg cgatccgccc cgaggcatgc accagcccct cgaccgtccc atcgttgaga
     2701 tcctggtggc tgacccggta gcctgcggcg gccagcatcg gaccgtcgag gacctccacc
     2761 tcgtggttat gggcaccgat gtccacgcgt ccggtggcca gctcgagcac cgcgtggttg
     2821 ccgccgtgat gaccgaccgc cagtcgccgc gtgcctgccc ccgcggccag cgccaggagc
     2881 tggtggccaa ggcagatacc caacaccggt gggccgcccg ccactgcgcg ctgagccagc
     2941 tgccgcacgg tctcgatctg tggcgccatg cgggaagggt cgccagggcc gggcgagagg
     3001 accacgaggt ctggctgggt tgccgccaca tcgacggcgg tcgcgtcatg cggcagcacc
     3061 tcgacctgca gcccgcgtcc ggtcagcgcg tgcagcaggg atcgcttcac cccgaagtcg
     3121 acgagggtgg cgcgcccgcg aagcgcacca accggtgaga ggctgtacgg cgtccgggcc
     3181 gagaccgccg cgacgtggtc gacggcctcc catccgatga tctggctcgc ctgctcgacg
     3241 gccccggact cctcagcggc ggcacggatg accgccttgc gcctcgcccc gcggcgcagc
     3301 tccagcgtca gggcacgggt atcgatcccc tcgagcgccg ggatgcccgc ctcgatcagc
     3361 agctcctcga aggtgcggcc cgggccggcc gggggcgtca ccagccgggt gacgatgacg
     3421 gccctgggat gcactcgatc ggactcgagc tcgttcggat cgatccggta gttgcccacc
     3481 atcgggtga
//
LOCUS       DATCQR010000262         8058 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_231284, whole genome shotgun sequence.
ACCESSION   DATCQR010000262 DATCQR010000000
VERSION     DATCQR010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 8058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8058
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231284"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1029)
                     /locus_tag="VIN32_08515"
     CDS             complement(<1..1029)
                     /locus_tag="VIN32_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HEY8922657.1"
                     /translation="MSIAAPVERHGLTEAEYGLAVEALGRELNLVELGMFGAMWSEHC
                     AYKHSRPLLSGLPSSGDRVLVGPGENAGVLDIGDGLAVVFKVESHNHPSAVEPYQGAA
                     TGVGGIIRDIFTMGARPIALLNSLRFGPVQPSEDATGRTDEAAATRNRFLMGGVVAGI
                     AGYGNCIGIPDVGGEIGFDASYNGNPLVNAMCVGIARHEEITLARAAGPGNALLLVGA
                     STGRDGIQGASFASANLGEDSEERRPAVQVGNPFLEKLLMEACLDLSSSDSVVAMQDL
                     GAAGLTCALAELSARGGCGAEVDLDLVPRREPGMSPYEVLLSESQERMLLVVRAGDEA
                     AVQASFERY"
     gene            complement(1053..2027)
                     /locus_tag="VIN32_08520"
     CDS             complement(1053..2027)
                     /locus_tag="VIN32_08520"
                     /inference="COORDINATES: protein motif:HMM:NF024183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEY8922658.1"
                     /translation="MQDDERDHGDAAAEQPIESAEAPTESAPSEAEQPIESAEAPTES
                     APSEAEAWVVPPPPTPSDPDVIPAHPVPATPLSEGTVCPRCGTDNRPGIAFCRACGQR
                     LMAPGAPAAVERPSPPEGTQACPRCGTHNRAGVPFCQNCGANLRPSDTAAEPALTPAA
                     TTAQTIEAPRRAILGPVVLLIGAAGMLTGWLLPFAFGGASLWDRSYGAPGGYGIGFWQ
                     GYPGGPISETAYFGLAAQVPILVALLVILAVAGAVRAAPGIVQRIGLFVALAWALALV
                     ALFLVVELIGGPGGDLIGVLRALSPGGIIFLLAGLIVTIGTLTRLWRA"
     gene            complement(2070..2813)
                     /gene="purQ"
                     /locus_tag="VIN32_08525"
     CDS             complement(2070..2813)
                     /gene="purQ"
                     /locus_tag="VIN32_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011058021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HEY8922659.1"
                     /translation="MSRSPRVAVLVFPGTWSDRDFVHVASVVGWETRLVWHTETDLGD
                     ADAVVLPGGFAHGDHLRTGAIARFSPVMAAVEAFAAAGGAVLGSCNGFQILTEAGILP
                     GVLLRNDHLQFRCDWQPLRVEQTAGPWLGELDSGDTIRLPISHGEGRYYAADAVLDEL
                     EASGRVALRYADADGSVTPDANPNGSLRSIAGIVNERGNVLGLMPHPERAAEADVGGT
                     DGLRILRGLDAWLAMQPAAAERRPAERRL"
     gene            complement(2810..3085)
                     /gene="purS"
                     /locus_tag="VIN32_08530"
     CDS             complement(2810..3085)
                     /gene="purS"
                     /locus_tag="VIN32_08530"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HEY8922660.1"
                     /translation="MRWLAEVHVRLRSGIADPEGQTIAGGLRALGFEVADVRAGKLLR
                     VTFEAPDRASAEAVVAKMCSRLLANPVMETADWELSAQESVAVGVDS"
     gene            complement(3085..3984)
                     /locus_tag="VIN32_08535"
     CDS             complement(3085..3984)
                     /locus_tag="VIN32_08535"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008476301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEY8922661.1"
                     /translation="MTLTESTIPGLALLHRGKVRETYAVGEDRLLLVATDRLSAFDVV
                     FEQPIPDKGRLLTALSAFWFRQLESLGPTHFLSTDADQLPPAARSSELAGRVTLARRA
                     ERIDAECVVRGYLAGSGWAEYRAGGEVGGHRLPEGLREADRLPEPIFTPSTKAEVGHD
                     ENITREQLAEMVGTELALELEERSLALYRAGARRAAEVGLILADTKFEFGWIDGAVAL
                     IDELLTPDSSRFWDAGQYKPGSSPPSFDKQYVRDFVAGSGWDREPPAPRLPDEVIEGT
                     RERYATAYERLTGEAWRADEGSA"
     gene            complement(3981..4553)
                     /locus_tag="VIN32_08540"
     CDS             complement(3981..4553)
                     /locus_tag="VIN32_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004380531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEY8922662.1"
                     /translation="MVAGVRGAPDGGRAGVDGGTALTPELGFNLELELAMRMAIGLVL
                     GAVIGFERELHHQPAGFRTHSLVAVGSALFTIISAYGFAGSAVDPTRIAAQIVSGIGF
                     IGAGTILQHRGSVRGLTTAASLWSVAAIGMAAGAGLLAIATVGTLLILVVLSVFDRIE
                     DFTRRRLGIESHLPEPMDAPSADGRTEDLS"
     gene            complement(4487..5407)
                     /locus_tag="VIN32_08545"
     CDS             complement(4487..5407)
                     /locus_tag="VIN32_08545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8922663.1"
                     /translation="MSTEGSWSRNAAVAASVATGRGDLWLPGALGALTYLAWLPLVIT
                     VAAAPRTSDLAFLGAGLLSSALFPLNVVLIAVVAALGLLIACLIASLAEASLLRATGL
                     GTPGRSMAREVEVSFSVMLLAVLPAVAVGAALISAAAAVGPAEFGSPDLGVPLALRMA
                     LRLAPLLAAFILLAWFGQAFGAVALRKAIGPGALPVGEAAKAALRDLLRRPVRRLGLA
                     LTAFITDLIAVALAAVLLRTLWAPIGAHVPSGQLVSPTALLLLVGFVAIWLAVILGFG
                     ALHVLFSTWWSLESGALRTEAAPESMEAQP"
     gene            complement(5404..6720)
                     /gene="purB"
                     /locus_tag="VIN32_08550"
     CDS             complement(5404..6720)
                     /gene="purB"
                     /locus_tag="VIN32_08550"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEY8922664.1"
                     /translation="MIDRYALPELRDLFSEQHKLDLWLRIELLAVEARHSAGLVPDDD
                     WERIRATAGRVDGARAHEIEQESQHDVIAFLRSVTERMGPEGRWLHFGLTSSDLLDTA
                     TAVVLRDAAAFVEGELAKLAELLAGMAVEHRSTPMVGRSHGIHAEPITFGFKAAGWLA
                     EVQRDQARLARAREGIAVGKVSGAVGTHAHVSAEVEEFVCRSLGLGIDAASTQVVSRD
                     RHAELLTAIAILGGTLERMAVEVRHLQRTEVGEAFEPFGAGQQGSSAMPHKRNPVLAE
                     RITGLARLLRADALVGLENMALWHERDISHSSAERFVFERALGVAGYATRSMTGLLGG
                     LEIDAGRMASNLELLGGMVHSEALLLAMIAKGADRQEAYRLVQGAAKRAWSGESTFRA
                     ELGADAEVGHWLSAGEVEEAMTMQPHLDGIGASYRALGLAADGGGG"
     gene            complement(6717..7208)
                     /gene="purE"
                     /locus_tag="VIN32_08555"
     CDS             complement(6717..7208)
                     /gene="purE"
                     /locus_tag="VIN32_08555"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012141680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEY8922665.1"
                     /translation="MSSTPIVAVICGSRSDLPALKGCFDVLDDYGISWEASVISAHRQ
                     PDALAAYVRDAEGRGVRIFVGAAGMAAHLPGVLASLTARPVIGVPLDAGALGGADALY
                     SVVQMPPGVPVAAVAIGSGGAKNAAHLAARILALGDPTLATRVDAVRATLAEAAELDI
                     DPR"
     gene            7316..7735
                     /locus_tag="VIN32_08560"
     CDS             7316..7735
                     /locus_tag="VIN32_08560"
                     /inference="COORDINATES: protein motif:HMM:NF017970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HEY8922666.1"
                     /translation="MNKQMVEEAPIGARIADKVTGFMGSWRFLILQTVLVAVWLAGNI
                     YLLSKPFDPYPFILLNLAFSTQAAYAAPLILLAGNRSAQRDRLTLEHAADETDVEEKQ
                     NVRLLRGNKEILEHVDALEHRILQLEERIMAALKPAG"
     gene            complement(7732..>8058)
                     /locus_tag="VIN32_08565"
     CDS             complement(7732..>8058)
                     /locus_tag="VIN32_08565"
                     /inference="COORDINATES: protein motif:HMM:NF014856.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide synthetase C
                     domain-containing protein"
                     /protein_id="HEY8922667.1"
                     /translation="MEGSLALKQGASVGVVVAAEGYPSEPVTGRRIEGAEPAAADDGG
                     PLLCFHAGTSRMADGSYASKGGRVVTFVGLDGDLADAREIAYRGVAACQLEGGQHRTD
                     IAVREL"
BASE COUNT         1287 a         2958 c         2720 g         1093 t
ORIGIN      
        1 ataacgctcg aaggatgcct gcaccgccgc ctcgtcacct gcgcgcacta cgagcagcat
       61 ccgctcctgc gattcgctca acaggacctc gtaggggctc atcccgggct cgcggcgcgg
      121 gaccaggtcc aggtcgacct cggcgccgca accaccgcgc gccgacagct cggcaagtgc
      181 gcaggtcagg ccggccgcac ccaggtcctg catcgccact accgaatcgg aggaggagag
      241 gtcgaggcag gcctccatca gcagcttttc gaggaacggg ttgccgacct gcaccgccgg
      301 gcggcgctcc tcgctgtcct ccccgagatt ggccgatgcg aagcttgcgc cctggatccc
      361 gtccctcccg gtcgacgctc ccaccagcag cagggcattg ccggggcccg ccgcccgtgc
      421 cagggtgatc tcctcgtgcc gggcgatccc gacgcacatc gcattgacca gcgggttccc
      481 gttgtatgag gcgtcgaagc cgatctcgcc gccgacatcg ggaatgccga tgcagttgcc
      541 atagccggcg atccccgcca ccaccccgcc catcaggaag cggttgcggg tggcagcagc
      601 ttcatcggtc cggccggtcg catcctccga tggctggacg ggaccgaaac gaagcgagtt
      661 caggagcgcg atcgggcgcg cccccatggt gaagatgtcg cggatgatgc ccccgacccc
      721 ggtggccgcg ccctggtagg gctccacggc gctggggtgg ttgtgcgact cgaccttgaa
      781 gacgaccgcc agcccgtcgc cgatgtcgag gacgccggca ttctccccgg ggccgacgag
      841 cacccggtct ccactcgagg gaaggccgct cagcagcggg cgagagtgct tgtaggcgca
      901 gtgctcgctc cacatcgcgc cgaacatgcc cagctcgaca aggttcagct cgcggccgag
      961 cgcctcgacg gccagcccgt actcggcctc ggtcagcccg tggcgctcca cgggcgctgc
     1021 gatgctcact ggctggggct caccctcgag cgtcaggctc gccaaaggcg cgtcagggtg
     1081 ccgatggtga cgatcagccc tgccagcagg aagatgatcc cgcccggcga cagggctcgc
     1141 aggacgccga tgaggtcgcc accagggcca ccgatcagct cgaccacgag gaagagggcg
     1201 accagcgcga gcgcccaggc gagcgcgacg aacagcccga tccgctgcac gatccccggt
     1261 gcggcccgca ccgccccggc cacggccagg atcaccagca gtgcgaccag gatcgggacc
     1321 tgagctgcca atccaaagta ggcggtctcg ctgatcggcc cgcccgggta tccctgccag
     1381 aagccgatcc catagcctcc cggagcgccg tatgagcggt cccacaggga tgcgccgccg
     1441 aaggcgaacg gcagcagcca gccggtcagc atgccagccg ccccgatcag cagcaccacc
     1501 gggccgagga tcgcccgacg aggagcctcg atggtctgag cggtggtcgc ggcaggagtc
     1561 agagcgggct cggccgcggt gtcggagggg cgcaggttgg cgccacagtt ctggcagaag
     1621 ggcacgccgg cgcgattgtg ggttccgcag cgtggacagg cctgcgtccc ttccggtgga
     1681 ctcggtcgtt ccacggcagc cggagcgccc ggggccatca gacgctggcc gcaggctcgg
     1741 cagaaggcga tccccggacg gttatccgtg ccgcagcgtg ggcagacggt cccctcgctg
     1801 agcggcgtgg ccggcaccgg atgcgcgggg atcacatcgg gatcggatgg ggtcggcggg
     1861 ggcggtacca cccacgcttc ggcctcgctt ggcgctgact cagtcggcgc ctcggccgac
     1921 tcaatcggct gctcggcctc gcttggcgct gactcggtcg gcgcctcggc cgactcgatt
     1981 ggctgctcgg ccgcagcgtc gccgtggtcc cgctcgtcat cctgcatcgg gcgatggacc
     2041 tccatcagga ccgcttcgga ggcctcgaat catagccgac gctccgccgg ccgacgttcc
     2101 gccgccgccg gctgcatggc cagccacgcg tccaggccgc gcaggatcct caggccgtcc
     2161 gtgccaccaa cgtctgcctc tgccgcacgc tcggggtgcg gcatcaggcc gagcacgttg
     2221 ccgcgctcgt tgacgatccc ggcgatcgat cgcagcgatc cgttcgggtt ggcatcaggc
     2281 gtgacgctgc cgtccgcgtc ggcgtagcgc agggcgacac gcccgctcgc ctccagctcg
     2341 tcgaggaccg cgtccgccgc gtagtaccga ccctcgccat gcgagattgg cagccggatc
     2401 gtgtcgccgg agtccagctc gccgagccag ggaccggcgg tttgctcgac ccgcagcggc
     2461 tgccaatcgc agcgaaactg caggtggtcg ttgcgcagca ggacaccggg caggatcccg
     2521 gcctcggtca ggatctggaa gccgttgcac gagccaagga ccgcaccacc cgcggcggcg
     2581 aaggcttcga ccgccgccat caccggcgag aagcgggcga tcgctccggt ccgcagatga
     2641 tcgccgtgtg cgaagccgcc gggcaggacc actgcgtcgg cgtcaccgag atccgtctcc
     2701 gtgtgccaca ccagccgcgt ctcccagccg acgacactgg cgacgtgcac gaagtcccga
     2761 tcgctccacg tccccgggaa gacgagcacg gcgactcgcg ggctgcggct cacgagtcga
     2821 cgcccaccgc cacggactcc tgggcgctca gctcccagtc ggcggtctcc atgacggggt
     2881 tggcgaggag ccggctgcac atcttcgcca ccaccgcctc ggcgctcgcg cgatcggggg
     2941 cctcaaaggt gacgcggagc agcttgccgg cgcgcacgtc ggcaacttcg aatccgagcg
     3001 cccgcagccc accggcgatg gtctgaccct cgggatccgc gatccccgag cgcagccgaa
     3061 cgtggacctc cgccagccag cgcatcaggc cgatccctca tccgcccgcc atgcctcgcc
     3121 tgtcagtcgc tcgtaggcgg tggcgtaccg ctcccgcgtc ccctcgatca cctcgtccgg
     3181 caggcgcggg gcaggcggct cgcggtccca gcccgatccg gcgacgaagt cgcgcacgta
     3241 ttgcttgtcg aacgacggcg ggctcgagcc cggcttgtac tgcccggcat cccagaagcg
     3301 cgacgagtca ggggtcagaa gctcatcgat gagcgccacg gcgccgtcga tccagccgaa
     3361 ctcgaacttg gtgtcggcca ggatcagccc cacctcggcc gcccgccggg cgccggcccg
     3421 atacagggcc agcgaccgtt cctccagttc gagggcgagc tccgtcccga ccatctcggc
     3481 cagctgctcg cgggtgatgt tctcgtcatg gccgacctct gccttggtgg agggcgtgaa
     3541 gatcggctct ggcaggcgat ccgcctcgcg caggccctcc ggtagccggt ggccgcctac
     3601 ctcgccgccg gcccgatact cggcccagcc agatcccgcg aggtagccac gaacgacgca
     3661 ttcggcgtca atccgctcgg ctcgtcgcgc gagagtgacc cgtcccgcca gctcagagct
     3721 gcgggctgcg ggcggcagct ggtccgcatc ggtcgacagg aagtgcgtcg gacccagcga
     3781 ttcaagctgc cggaaccaga acgccgacag cgccgtgagg agcctcccct tgtcggggat
     3841 cggctgctcg aagaccacgt cgaatgccga gaggcgatcg gtcgcgacca gcagcaggcg
     3901 atcctcgcct accgcgtacg tttcgcgcac cttgcctcga tgcagcagtg ccagccccgg
     3961 gatggtcgac tcggtcaggg tcatgacagg tcctctgttc ggccgtcagc cgacggcgca
     4021 tccatcggct caggcaggtg cgactcgatt ccaaggcgcc gccgcgtgaa atcctcgatc
     4081 cggtcgaaca ccgacagaac taccaggatc aggagggtgc ccacggttgc gatggccaac
     4141 aggccagctc ctgccgccat gccgatggct gccaccgacc agagcgaggc ggcggtggtc
     4201 aacccgcgga cgctgccgcg atgctggagg atggtgcccg cgccgatgaa gccgatcccg
     4261 gacacgatct gggcagcgat ccgggtcgga tcgaccgcgg acccggcgaa cccgtacgcc
     4321 gagatgatgg taaagagcgc cgatcccacc gccaccagcg agtgcgtgcg gaagcctgcg
     4381 ggctggtggt gcaactcgcg ttcgaagccg atcacggccc cgagcaccag gccgatcgcc
     4441 atccgcatgg ccagctcgag ctcgaggttg aagcccagtt cgggggtcag ggctgtgcct
     4501 ccatcgactc cggcgcggcc tccgtccgga gcgccccgga ctccagcgac caccaggtcg
     4561 agaacaggac gtggagcgcc ccgaagccca ggatcaccgc cagccagatc gccacgaagc
     4621 ctaccagcag caggagcgcc gtcgggctca ccaactgccc gctcggcaca tgagcaccaa
     4681 tcggcgccca gagggtgcgc agcagcaccg cggccagggc gacggcgatg aggtcggtga
     4741 tgaacgccgt gagtgccagc ccgagccgac gcaccggtcg gcggaggagg tcgcgcaggg
     4801 ccgccttggc cgcctcgccg acgggaagcg cacccggccc gatcgccttt cgcagcgcca
     4861 ccgccccgaa ggcctgcccg aaccacgcca ggagaatgaa tgccgcgagc aggggcgcga
     4921 ggcggagcgc cattcgcagc gccagcggga cgcccaggtc gggcgagccg aactcggctg
     4981 gccccacggc cgccgcagcg ctgatcagcg ccgcgccgac cgccacggcg ggcagcaccg
     5041 ccaacagcat cacgctgaag ctgacctcca cctcccgtgc catcgaccga cccggtgtgc
     5101 caaggccggt cgcacggagg agcgacgcct cggccaggga cgcgatcagg caggcgatga
     5161 ggagccccag cgccgcgacg acggcgatca gcacgacatt gagcggaaac agcgccgagc
     5221 tcaggaggcc cgcaccgagg aaggcaaggt cgctggtgcg tggggctgcg gccacggtga
     5281 tcacgagcgg cagccaggcc aggtaggtga gggcgcccag cgcccccggc agccacaggt
     5341 ccccacgacc tgtggccacg cttgccgcga cggcggcatt ccgggaccag ctgccctccg
     5401 tgctcatccc ccgccgccgt ccgccgcaag tcccaatgcc cggtagctcg ccccgatccc
     5461 atccaggtgc ggctgcatcg tcatcgcctc ctcgacctcc ccggcagaca gccaatgacc
     5521 tacctccgcg tcggcaccaa gctccgcgcg gaaggtgctc tcccccgacc acgcgcgctt
     5581 ggcggcaccc tgcaccaggc gataggcctc ctgacggtcg gcgcccttgg cgatcatggc
     5641 cagcaggagc gcctccgagt ggaccatgcc gccgaggagc tcgaggttgc tcgccatgcg
     5701 tcccgcatcg atctccaggc cgcccagcag cccggtcatg gatcgggtgg cgtagccggc
     5761 gacccccagg gcacgctcga agacgaagcg ctcggcgctg gagtggctga tgtcgcgctc
     5821 atgccacagc gccatgttct cgaggccgac cagcgcatcg gcccgaagca ggcgagcgag
     5881 cccggtgatc cgctccgcga gcacggggtt gcgcttgtgg ggcatcgcgc tgctcccctg
     5941 ctggccggcg ccgaagggct cgaacgcctc gccgacctcc gtgcgctgca ggtggcgaac
     6001 ctcgacggcc atccgctcca gcgtcccgcc caggatggcg atcgccgtga gcagctcggc
     6061 gtgccgatcg cggctcacca cctgggtcga ggccgcgtca atgccgagcc cgagtgagcg
     6121 gcacacgaac tcctccacct ccgcgctgac atgggcgtgc gttcccaccg ccccggagac
     6181 cttgccgacg gcgatcccct cccgggcgcg tgccagccgc gcctggtcgc gttgcacctc
     6241 tgccaaccag ccggccgcct tgaacccgaa ggtgatcggc tcggcatgga tcccgtggct
     6301 gcgtccgacc atcggggtgg accgatgctc gacagccatg ccggccagca gctctgccag
     6361 cttggccagc tcgccttcca cgaacgcggc cgcgtcccgc agcaccaccg cggtggcggt
     6421 gtccaggagg tcggagctgg tcaacccgaa gtgcagccag cgtccctcgg ggcccatccg
     6481 ctcggtgacc gatcgcagga aggcgatcac gtcgtgctgc gactcctgct cgatctcgtg
     6541 ggcgcgggcg ccgtcgacgc gcccggcggt ggcgcggatt cgctcccagt cgtcgtccgg
     6601 caccagtcca gctgaatgcc tggcctccac cgccagcagc tcgatccgca gccagaggtc
     6661 cagcttgtgc tgctccgaga agaggtcgcg aagctcgggg agcgcgtatc ggtcgatcat
     6721 cgggggtcga tgtccagctc ggccgcctcc gccagcgtgg cgcgcaccgc gtccacccgc
     6781 gtggccagcg tcggatctcc gagcgcaagg atgcgggctg ccaggtgcgc ggcattcttc
     6841 gccccgccgg acccgatggc gaccgccgcc accgggacgc cgggcggcat ctgcaccacg
     6901 ctgtacagcg catcggcgcc gccgagggcg cccgcgtcga gcggaacgcc gatcaccggt
     6961 cgcgcggtca gggaggcgag cacgccgggc aggtgggccg ccatgcctgc ggcgccgacg
     7021 aagatgcgca cccctcgtcc ctctgcgtca cgaacgtagg cggcgagcgc atccggttgg
     7081 cgatgggcgc tgatcacgct cgcctcccat gagatcccgt agtcgtccag gacgtcgaag
     7141 cagcccttga gcgcgggcag gtcggaacga gagccgcaga tgacggcgac gatgggggtg
     7201 gagctcatcg gtcctaggat atcccgcatg cgagcggccg tcggccgagc gcgggcgagt
     7261 ctggaggaat gccatgtcga cgaggtccac cacgaggccg gcgtaccggc atccggtgaa
     7321 caagcagatg gtcgaggagg cgccgatcgg ggcgcgcatc gccgacaagg tcaccggctt
     7381 catgggcagc tggcgattcc tgatcctgca gaccgtgctg gtggccgtct ggctggccgg
     7441 caacatctac ctgctcagca agccattcga tccctacccg ttcatcctgc tgaacctggc
     7501 cttctcgacc caggccgcct atgcggcgcc gctgatcctc ctggcaggaa accgctcggc
     7561 gcagcgagac cgcctgacgc ttgaacacgc cgccgacgag accgatgtgg aggagaagca
     7621 gaacgtgcgg ctgttgcgcg gcaacaagga gatcctcgag cacgtcgacg cactcgagca
     7681 ccggatcctc cagctcgagg agcggatcat ggcggctctc aagcccgccg gctagagctc
     7741 gcggacggcg atgtcggttc gatgctgacc gccctccagc tgacaggcgg cgacgccgcg
     7801 gtaggcgatc tctcgtgcgt cggcaaggtc gccgtccagc ccaacgaagg tgaccactcg
     7861 cccgcccttc gatgcgtacg aaccatccgc catgcggctc gtgccggcgt ggaagcacag
     7921 cagcgggccg ccatcgtcag ccgcggcggg ctccgcaccc tcgatgcgcc gccctgtgac
     7981 cggctctgac ggataaccct cggcggccac caccacaccg accgatgcgc cctgtttgag
     8041 cgccagtgag ccctccat
//
LOCUS       DATCQR010000263         5164 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_216631, whole genome shotgun sequence.
ACCESSION   DATCQR010000263 DATCQR010000000
VERSION     DATCQR010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5164
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216631"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /locus_tag="VIN32_08570"
     CDS             <1..552
                     /locus_tag="VIN32_08570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8922668.1"
                     /translation="SGNWKPGIYNGFAPSGGTMNGGVYKIINATNLSLGTITNTIYTP
                     SGTSDPTGAVAIVLDSSDTATLDITDAKLNGLDDLYPVNYTGTRDPMGTHNFVIFGGN
                     GPTGFAGSMAVGPAATTDLSGIIYLPEVAYSSNGNSSPQFTGSVMVASMTVTGGGNGV
                     QVFHWVCGLQAVAGQPWQGGLLR"
     gene            610..2040
                     /locus_tag="VIN32_08575"
     CDS             610..2040
                     /locus_tag="VIN32_08575"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEY8922669.1"
                     /translation="MIPNLIGSEWRSPDVNDSLPIYNPATGEVIGQVPLSGAKEVDAA
                     VQAAVAAYKSWSRTALMERVRLMFRYKALLEEHFEDLAAIITRHHGKTLEESRGEVRR
                     GIEVVDFACGAPTLLQGRTLRDVSGGVDQDLYRYPVGVCAGIPPFNFPVMIPLWMFPL
                     AVVAGNTFVLKPSERTPLGAARLAEIFLEAGFPEGVLNVVHGGSEAVNALLVHPGVNA
                     ISFVGSAPVARHVYTTAAAHGKRVQALGGAKNHIVVMPDADPEIAMPAILNSAFGNAG
                     ERCLAGSVAVAVGEAAEGLIGPLAKAAAKMVVGPGDQEGVQVGPLIRADHRDRVAGWV
                     ERGVAEGAELLVDGRGQMSRPGFFLGPTILDNVTSEMAVGREEIFGPVLSVARAATLD
                     DAIAQANRMSLGNMATIFTQSGKSGREFRERVEAGMTGINVPIAQPFAFFPFSGWKGS
                     FYGDLHVHGTDGIEFYTRKKVVVTRW"
     gene            complement(2044..2904)
                     /locus_tag="VIN32_08580"
     CDS             complement(2044..2904)
                     /locus_tag="VIN32_08580"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY8922670.1"
                     /translation="MAIARARFGRTEHQSSRVIFGGAALSSVSQDVADRTLDVLLEHG
                     INHIDVAASYGDAELRVKPWLRGARDSFFLATKTDERKAVQAREELHRSLDRLGVDHV
                     DLWQLHNLADPIEWDTALSPGGAIEAAVEAREQGLVRWIGVTGHGAQIAANHRRSIAR
                     FDFDSVLLPYNFVTMQSPYYAENFEALVAACTERGTAVQTIKSIALRPWSGLTHTRST
                     WYQPLEEQADIDLAVSWVLGRPGVFLNTVGDVDLLPRVLDAAGRFEKRPDDAAMKSLL
                     ERSKTEPLFV"
     gene            complement(2965..4152)
                     /locus_tag="VIN32_08585"
     CDS             complement(2965..4152)
                     /locus_tag="VIN32_08585"
                     /inference="COORDINATES: protein motif:HMM:NF024241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8922671.1"
                     /translation="MPSTKRPRGALYGIVAAVFVSGTGIGSLLPILPLFLRERGASYS
                     FVGLIVGAALAAQAVGQWPAGWLAERIGRKEMMVVGLLVASAASLMFLLPLSTEWLVA
                     VRFLQGLGFAAAAPAEIAAVADVVPPEELGRAYGWVSAARQSGFIVGPALGGVLAVFG
                     RWTVFAVTGGALATAALVAALTLPRSVRRPRAEVSIGRVFMRSTRVGVAMRAVVTMTV
                     GLGLLIGIYDVIWSLYMRQIGASDFIIGLSFTFFALPLVAATPFAGWASDRWDRRWLA
                     FTSVALGSLMAVIYPFVNFIPVVIGVGVIEASFWAFTEPAMNSFLMEAVRDRRGEAQG
                     VVGTAQSGSMAIGSLIGGSLFALGLGVPFFVAAAVGFGFALAALPGLRAAGRRAPIIQ
                     EPA"
     gene            4197..>5164
                     /locus_tag="VIN32_08590"
     CDS             4197..>5164
                     /locus_tag="VIN32_08590"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY8922672.1"
                     /translation="MANDRADLVVVGAGTVGGWASYFAATQGAERVVVIERGRAGSGA
                     SSRAAGIVRAQGGTPTTVGLGRWSIDFYRGQQARLGTDSGFRELGYLIFAGTDHEARV
                     GRERVSMQRSAGLDVEWLEPDAVVKHNPALAPDSFVGASYLATDGCIDPPRNVLAYSL
                     AMQQAGVHLREHTTFVGLRTERASGGNRLTGVETSAGLIETSRAILTGGPTLREVGRA
                     AGVEIPAGAVRHQVAVTEPHAAFDVERQAMGFDLGAGLYWRLEEGGLLFGMSNPDEPP
                     GPSREIDWPYMDEMRSRLAHYLPVTAGLGLRKAWAATIDYTPDHQP"
BASE COUNT          919 a         1733 c         1716 g          796 t
ORIGIN      
        1 tcgggcaact ggaagccggg catctacaac gggttcgcgc cgagtggcgg cacgatgaac
       61 ggcggcgtct acaagatcat caacgccacc aacctctcgc ttggcaccat caccaatacc
      121 atctacacgc cgtccgggac ttccgatccg acgggcgcag tggcgatagt gctcgactcg
      181 tcggacaccg ccacgctcga tatcaccgat gccaagctca acgggctcga cgacctttac
      241 cccgtgaact acacagggac tcgcgacccg atgggcaccc acaacttcgt gatcttcggc
      301 gggaacggcc cgaccgggtt tgcaggctcg atggcggtgg gtccggccgc gaccaccgac
      361 ctgagtggca tcatctacct gcccgaggtc gcctacagca gcaacggcaa ctcgagcccg
      421 cagttcacgg ggtcggtgat ggtggccagc atgacggtta ccggcggcgg caacggcgtg
      481 caggtattcc actgggtctg cggcctgcag gcagtagccg ggcagccctg gcaggggggc
      541 ctgctccgct agcaaacaaa cccggctgca ctcgacgccg gtcattggga aggcgctaga
      601 ttgaggccga tgatccccaa cctcatcggc tccgagtggc gcagccctga cgtcaatgac
      661 tccctaccga tttacaaccc ggcgacgggc gaggtgatcg gccaggtccc gctatcgggc
      721 gccaaagagg tggacgccgc cgtgcaggcg gctgtcgccg cttacaagag ctggtcgcgc
      781 accgcgctca tggagcgcgt gcgcttgatg ttccgctaca aggcgctgct cgaggagcac
      841 ttcgaggatc tggctgccat catcacgcgg caccacggca aaaccctcga ggagtcgcgt
      901 ggcgaggtac gccgcggcat cgaggtggtg gactttgcct gcggcgcgcc gacgctgctc
      961 cagggccgca ccttgcgcga cgtttcgggt ggagtcgacc aggacctcta ccgttatccg
     1021 gtcggcgttt gcgctggcat cccgcccttc aacttcccgg tgatgatccc gctctggatg
     1081 ttcccgctcg cggtcgtggc cggtaatacc ttcgtcctga agccgtccga gcgcactccc
     1141 ctcggggcgg cgcgcctggc cgagatcttc ctcgaggcgg gctttcccga aggcgtgctg
     1201 aacgtcgtgc atggaggcag cgaggcggtc aacgcgctcc tcgtgcatcc gggcgtcaac
     1261 gcgatctcgt tcgtcggctc tgcgccagtg gcgcgtcacg tgtacaccac cgccgcggct
     1321 cacggcaagc gggtccaggc tctaggggga gcgaagaatc acattgtcgt gatgccggat
     1381 gcggatccgg agatcgcaat gcctgcgatc ctcaactcgg ccttcggcaa tgcgggcgag
     1441 cgctgccttg cgggctccgt cgctgtcgcg gtgggggagg ccgccgaagg cctcatcggc
     1501 ccactcgcga aagctgccgc gaagatggtc gtcgggccag gcgaccagga aggcgtgcag
     1561 gtcgggccgc tgatccgcgc cgaccaccgc gaccgcgtcg ccggctgggt cgagaggggt
     1621 gtcgccgagg gggctgagct ccttgtcgac ggccgcggtc agatgtctcg gccaggtttc
     1681 ttccttggcc cgacgatcct cgacaacgtc acctctgaga tggcggtcgg tcgcgaggag
     1741 attttcgggc ctgtgctctc ggtggcaagg gcggccacgc ttgacgacgc gatcgcgcag
     1801 gccaatcgga tgtcgctggg caacatggcc acgatcttca cgcagtcggg gaagtcagga
     1861 cgggagttcc gggagcgcgt ggaagcgggg atgacgggca tcaatgttcc catcgcacag
     1921 cccttcgcct tctttccgtt cagcggttgg aagggctcgt tctacggcga cctccatgtc
     1981 cacggcacgg acgggatcga gttctataca aggaagaaag tcgtcgtaac ccgctggtaa
     2041 cgcctaaacg aacagcggct ctgtcttcga tctctccagc aggcttttca tcgcggcgtc
     2101 atcaggcctc ttctcgaacc gcccggccgc atcgagcacc ctcggcagca ggtctacgtc
     2161 gccgaccgtg ttcaagaaaa cgccgggccg tcccagcacc cacgagacag cgaggtcgat
     2221 gtcggcctgc tcctccagcg gctggtacca cgtcgatcgc gtatgcgtca ggcccgacca
     2281 cggccgcagc gctattgact tgatcgtctg caccgccgtc ccacgctccg tgcaagcggc
     2341 gacgagggcc tcgaagttct ccgcgtagta gggcgactgc atggtcacaa agttgtaagg
     2401 gagcagaacg gaatcgaagt cgaagcgcgc gatgctccgg cggtggttgg cagcgatctg
     2461 cgcgccgtgg cccgtgactc cgatccaccg gaccaggccc tgctcgcgcg cctcgaccgc
     2521 ggcctcgatc gccccacccg ggctgagggc ggtgtcccat tcgatgggat cggcgaggtt
     2581 gtgcagctgc cagaggtcga catggtcgac acccaaccgg tcgagcgatc gatgcagctc
     2641 ctcacgcgcc tgaacggcct tgcgctcatc ggtcttggtg gccagaaaga atgagtctcg
     2701 ggcgccacgc aaccagggtt tgacgcggag ctccgcatcg ccgtacgacg cggccacgtc
     2761 gatgtgattg atcccatgct cgagcagcac atcgagcgtg cggtcggcca cgtcctggct
     2821 gaccgatgaa agggcggcgc cgccgaagat cacccggctg ctctgatgtt cggtgcgccc
     2881 gaacctcgcg cgagcgatgg ccatcaagaa aatcttaggc gcaaatcgtg gcgctacgcg
     2941 ttgaggggcc gcccaggatg aggatcaagc cggttcctga atgatcggcg cgcgccggcc
     3001 cgcagctcgc aggcctggga gcgcggcaag ggcgaacccg aagccgacgg cggccgccac
     3061 gaagaacggc acacccagcc cgagcgcgaa gagcgagccg cctatcaacg atccgatcgc
     3121 cattgagccg ctctgggccg taccgaccac cccctgcgcc tcgcctcgcc ggtctcgcac
     3181 agcctccatc aagaacgaat tcatggcagg ctcggtgaac gcccaaaagc tggcctcgat
     3241 gacgccgacc ccgatcacga ccgggatgaa gttgacaaac ggatagatca ccgccatcag
     3301 ggagccgagg gcgaccgagg tgaacgcgag ccagcgccgg tcccagcggt cggatgccca
     3361 gccggcgaac ggcgtggcag cgacgagcgg cagcgcaaaa aaggtgaaag acagcccgat
     3421 gatgaagtcg ctcgcgccga tctggcgcat gtagaggctc cagatcacgt cgtagatccc
     3481 gatcagcagg ccgaggccga ctgtcatggt cacgaccgca cgcatcgcca caccaacgcg
     3541 cgtcgagcgc atgaagactc gaccgatgct tacctctgct cgtggccgcc gcaccgagcg
     3601 gggaagcgtc agcgccgcca ccagggccgc ggtcgcaagc gcgcctccgg tgactgcaaa
     3661 cacggtccac cggccgaaca ccgccagcac tccgccaagg gcggggccga cgatgaagcc
     3721 cgattgtcgc gccgccgaaa cccatccgta agcgcggccg agctcctcgg gtggcaccac
     3781 gtcggcaacg gcggcaatct cggccggcgc cgccgccgcg aaaccgaggc cctgcaggaa
     3841 gcgaacagcg accagccatt cggttgaaag agggaggagg aacatcagcg atgcggccga
     3901 agccaccagg aggccgacca ccatcatctc cttgcgcccg atcctctccg ccagccatcc
     3961 cgccggccac tggccgaccg cctgagcggc aagcgccgct ccgacgatga gtccgacgaa
     4021 ggagtacgac gcgccccgct cgcgcaggaa caacgggagg atcggcagca gcgacccgat
     4081 cccggtgccg gaaacgaaga ccgcggcaac gattccgtag agcgcgccgc ggggccgttt
     4141 ggtggaaggc atcccgaaag gttaaggggc cgcccctgtc cgacgataat ggcgcgatgg
     4201 caaacgatcg cgcggacctt gtcgtggtcg gagccggcac ggtcggcggc tgggcttcgt
     4261 attttgcggc cacccagggt gctgagcgag tcgtggtcat cgagcgcgga cgagccggta
     4321 gcggcgccag cagccgtgcc gcaggcattg tgcgtgcgca gggcggaacc ccgaccacag
     4381 tcgggcttgg tcgctggagc atcgacttct atcgcggcca gcaggctcgg ctgggaaccg
     4441 actccggttt ccgcgagctg ggttatctga tcttcgccgg caccgatcac gaagcgcgcg
     4501 tcggacgcga acgcgtgtcc atgcagcggt cggccggtct tgacgttgaa tggctggagc
     4561 cggacgccgt tgtgaagcac aatcccgcgc tggcgcctga cagcttcgtg ggggcaagct
     4621 acctcgctac tgacggatgc atcgatccgc cgcgaaacgt cctcgcctac tcgctcgcca
     4681 tgcagcaggc gggggttcat cttcgcgagc acaccacgtt tgtcggcctg cgcacggagc
     4741 gggcctccgg cggcaacagg ctcaccggcg tcgagacctc tgccgggctc atcgagacgt
     4801 ctcgggccat cctcacggga ggcccaaccc tgcgggaggt cgggcgcgct gcgggtgtgg
     4861 agattcccgc gggcgcggtg cgtcaccagg tcgcggtcac cgagccccat gcggccttcg
     4921 acgtcgagcg gcaagcgatg ggcttcgacc tcggcgcggg cctctactgg cggctggaag
     4981 agggcggcct gctattcggc atgagcaacc cggacgagcc cccaggtccc tcgcgcgaga
     5041 tcgattggcc gtacatggac gagatgcgat cgcggctcgc gcactacctg ccggtgaccg
     5101 cgggcctggg cctgcgcaag gcctgggcgg ccaccatcga ctacacgccg gaccaccagc
     5161 cgat
//
LOCUS       DATCQR010000264        11436 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_107765, whole genome shotgun sequence.
ACCESSION   DATCQR010000264 DATCQR010000000
VERSION     DATCQR010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 11436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11436
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107765"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(109..531)
                     /locus_tag="VIN32_08595"
     CDS             complement(109..531)
                     /locus_tag="VIN32_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008954788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3830 family protein"
                     /protein_id="HEY8922673.1"
                     /translation="MPNLTIRVGDLNFTARWEAEAPRTVEAVRRLLPMRSQLIHCRWS
                     GEGTWIPLGDLQTGVGYEHHTSHPSPGELLIYTGNLSECEILFPYGACMFSSKLGQLA
                     GNHFATLLPDEGWRDRLREVGRRVLWDGAHEIEIGEDA"
     gene            547..900
                     /gene="uraH"
                     /locus_tag="VIN32_08600"
     CDS             547..900
                     /gene="uraH"
                     /locus_tag="VIN32_08600"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007727227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HEY8922674.1"
                     /translation="MADLRPTISTHVLDLELGVPAAGLPVALFRLEQDGRPELITELA
                     TDADGRIPSLLPGELEVGDYQLVFDLAAYAATLDRDADFFENLAVGIRIDDAERSYHV
                     PLLLSPFGISAYRGT"
     gene            900..1430
                     /locus_tag="VIN32_08605"
     CDS             900..1430
                     /locus_tag="VIN32_08605"
                     /inference="COORDINATES: protein motif:HMM:NF020907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline
                     decarboxylase"
                     /protein_id="HEY8922675.1"
                     /translation="MGYLSIDDLNDLSADAFADEVAPLFEGAGGFLRRLAAARPFDSY
                     HSFLATAREVARTMPEADQIELLNAHPTLGGDSALVSAASFDEQGYGSPEEDGQPVLE
                     ELAMLNEVYEGRFGFRYVVFVAGRPPSAMGPLIEAAMRNDRAAELARGLDEVIEIAGD
                     RLGRMRGDYLDPEEGS"
     gene            1430..2359
                     /gene="pucL"
                     /locus_tag="VIN32_08610"
     CDS             1430..2359
                     /gene="pucL"
                     /locus_tag="VIN32_08610"
                     /inference="COORDINATES: protein motif:HMM:TIGR03383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004846 - urate oxidase activity [Evidence
                     IEA];
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urate oxidase"
                     /protein_id="HEY8922676.1"
                     /translation="MQYEIHYGKADVKVYRTYGPPLREVRQIPESPFGGRENTLMAAE
                     LEVTVHGTAFLDAYTRGDNRRVVATDTMKNFMHTASLASKAPTLEGWLHHVGSAFLAT
                     YPDMEHLTMHGRELPFPAALVPADDGDGFAASEVLFARDRNDRSTASLELERHEDGIR
                     IAGHRCGRTELQLIKITGSAFADFARDEHTTLPERPDRPLYVWSDIGWRYTDALDALG
                     GDPGRYVAGEQVADLAASVFHGFVSLSIQHLVNEIGIRMLERWPQLAEVSFDASNRLW
                     DTGVQSEADPRVKTYADPRPPFGKIGLVLRRDD"
     gene            complement(2362..2808)
                     /locus_tag="VIN32_08615"
     CDS             complement(2362..2808)
                     /locus_tag="VIN32_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020576865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEY8922677.1"
                     /translation="MTVQSKRWNDFEVARRILSEFRVWAVVGASPDPTRASFGVTRSL
                     MRFGFEVIPVYPRETTIHGLATVPDLRTAAAQRPAGSPIEVVDIFRASHRAGAHVDEA
                     IEIGARAVWLQLGVWDEEAAERAAAAGLLVVMDRCPAIDFPQLEPG"
     gene            complement(2805..3380)
                     /gene="ruvA"
                     /locus_tag="VIN32_08620"
     CDS             complement(2805..3380)
                     /gene="ruvA"
                     /locus_tag="VIN32_08620"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEY8922678.1"
                     /translation="MIGSLRGPVTHVGQEFVLVELGGVGYRVLVGPSLLAQLRLGSEA
                     RIFVHHLVREDQQALFGFASPEELAFFELLMTVTGVGPRLALAITSAHAVTKLQLAIV
                     TDDADLLTSVSGVGRKTALRIILELKEKIHAAGIAAGGGASTDSDVVAALESLGYTAS
                     EARRAAGAVAGGEDGLDLRIKAALQELARNR"
     gene            complement(3377..3874)
                     /gene="ruvC"
                     /locus_tag="VIN32_08625"
     CDS             complement(3377..3874)
                     /gene="ruvC"
                     /locus_tag="VIN32_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEY8922679.1"
                     /translation="MIALGIDPGTARCGFGVVALDDGRLRLIDAGVIRTESAETDADR
                     LRSLHSALTELFTLHHPHRVGVERLFFQRNVQTAMAVGQARGVALLAAAEAGLLVDEP
                     TPNEVKQAVCGNGAADKAQVASMVARLLGIALDGVADDATDALAVAIGCAYRGMTSAR
                     LAVIG"
     gene            complement(3900..4658)
                     /locus_tag="VIN32_08630"
     CDS             complement(3900..4658)
                     /locus_tag="VIN32_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY8922680.1"
                     /translation="MSGHSKWSQIKRQKGVNDARRGALFTKLTREIITAARQGGGDVD
                     GNYRLRMAIDKARDNSMPMENIKRAIERATGAGADAVQYEEITYEGLGPANVAVIVAV
                     MTDNRNRTASEVRSIFTKVGGSLTPVAWQFQQRGLLSIPLKGSDPDEVTLAAIDAGAT
                     DVDTPEGGVILVMTEPADLERVRSSLADAGHPATSAEVSMEPTTKVEISDEKAARQVL
                     GFVERLEELEDVQNVYANFDLPDALMEQLEASVA"
     gene            4728..5186
                     /locus_tag="VIN32_08635"
     CDS             4728..5186
                     /locus_tag="VIN32_08635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8922681.1"
                     /translation="MVPFLDRAENAGALRYLAHGRSDAEGFFGPPPADLDRRHLGTHP
                     TVVDRLWEELNQALPADARWLVFDGPALVHPGGSILAAAMGTQYALRLLTDDRAAAIA
                     AGAEVVHHFQTVGTTLDLPAMFGPEWVFGLWDEREPDWLRASYDAIGPAA"
     gene            5323..7398
                     /gene="fusA"
                     /locus_tag="VIN32_08640"
     CDS             5323..7398
                     /gene="fusA"
                     /locus_tag="VIN32_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008517311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEY8922682.1"
                     /translation="MKPTSPDRIRNVALISHGGAGKTSLAEAMLFDAGAIARLGSVDA
                     GSAALDHDPDEQKRRQSINLAIGTLEADDARITLVDTPGYADFQADVIEALAAVDAAI
                     LVVDASAGVEVGTEEVWRLAEERNLPRFIFVNKMDRENANYDAVLDGLKAKFGPKIAP
                     VYLPIGSAESFRGYIDVIEQHANVYESGNPSEVPIPDDMRAGEEARREALVEAAAEAS
                     DELMTRYLDGETISDEEIETAVHKGTREGSVVPVFVGSALKNIGVRELASMIAKHIPS
                     AAEVGARTTSDGKRIEPDPDAPLVAQVFKTTADPFVGRLTYFRIVAGTLHAQGHLWNA
                     TRKAEERIGNILGLLGKEQENMPQAGPGDIVAVAKLASTQIGDTLVADRNQAVSLPPL
                     EFPSPSLQVSIEPESKADLDKLGQALSRMLEEEPCARVHREEGTGETILTAMGDAHVD
                     VLVERLKRKFGASVRTGSPHVPYRETIRKPAKIDNRFKRQTGGHGQFGHVVIEFEPLP
                     SGSGFQFGDKIVGGVVPRQYIPAVEKGLRETLAEGVLAGYPVVDLSARLVDGSYHTVD
                     SSEMAFKVAASQALRRAFPEADPTLLEPILEVEVTVPDAYMGDVMGQITAKRGHVLGM
                     DSSDGMQHLRAQVPQAEMFHYATELRSITQGRGRFSWKLDHYAEVPSTISDRVIADHQ
                     AKVGAGEKH"
     gene            complement(7482..8702)
                     /gene="tyrS"
                     /locus_tag="VIN32_08645"
     CDS             complement(7482..8702)
                     /gene="tyrS"
                     /locus_tag="VIN32_08645"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEY8922683.1"
                     /translation="MKIDKREAAVDALLSNRVAQVVEHDELRRLLLAGDRPLRVKFGV
                     DPTSPDLHIGHGVPFERLRAFQDLGHLPVLVVGDTTAQIGDPSERNSTRPMLTADQVR
                     ANAETYLAQFGLIVDPERTEVRWQSEWFGRFGLGDVFKLLSDFTLARIIERDTFAKRL
                     KDGIPISMHETLYPLLQAYDSVAVQADVELGGTDQTFNLLVGRDLLRDHGQAPQQIMT
                     VELLVGTDGVQKMSKSLGNAIGLTDAPYEQYAKTMSIPDELMDNWAHLVTRWTNAEAD
                     SLVAALSAGEIHPKAAKQRLAREIVSRWHGEGAAESAAEEWERAIGRGEGITDIATID
                     LAAPDGQLSLIQIMTMSGLAGSNTQVRNLVRQGGVRLNGEVVASPDIPTFAPGDQPEI
                     RVGKRVGKRIRVTK"
     gene            8904..>11436
                     /locus_tag="VIN32_08650"
     CDS             8904..>11436
                     /locus_tag="VIN32_08650"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEY8922684.1"
                     /translation="MLAGALFIGVVAVFASYASGLPDPSQLAHFELSQGSSVMSADGV
                     ELATFAAEQRRVLTYDKIPQVMADAQVAAEDQTFWSNPCIDFKGIVRAALQNFSASQT
                     VSGASTICQQLVRIRLFDADLLANPDRRWERKIKEALLALQVGDAYPGTEGKQKLLEM
                     YLNQVYYGNNAYGIWAAANRYFGKDITSSAPENQLSVGEAALLAGLVRAPSALDPTQV
                     AVPSTDAAGNTILVVPPDADAIRVQGYVLNGMVKTGAITPAERDQAAAEQIVLVPPQS
                     RQYKAPHFVYAVRRAAADLLGNEDLLDRGGLVISTTLNYNGYQVSAEKWAGVAYDLDR
                     LSDQELTAKYGEAALAWIKQLQGRNINNDALVTINYRTGAVMAYVGSANYYGEATAAH
                     QPQYDVVGQAYRQSGSAFKPITYATGFERGTITPATMLMDVKGIIAEGYSVPNASGNE
                     RGPVRVRDALKYSWNIPVAKAQQLIGTQNVVDMAKRLGLSWDPQQKDVAVPSLTLGTI
                     GVHMLDLAGAYGAIANGGTLSKPFLIEKITDRDGTVIYDHGADAPKPERVLSEDAAYL
                     VTDILADNTNPATNIIWGQRFQLSTDAGRRPATLKTGTTNDFRDLQAFGYLAPDKDPK
                     VDEGAIITGVWVGNSDFSAIKDVFAADGPTFIWHDYMAEVSAANKLPSRDFPRPADIV
                     SLKVDALTGMLPGDFTTETVTEDFAAAHQPTERDTLHRKLAIEEATGKIWQPGCGDPT
                     AASAAPGASPGSQAPEEKVYLDLVDYEASHPTWEEANRTWIERYIGKEDQVRRSPAPA
                     FDAPLAPIEKCTPGEIPTSTPWPSPSPTPSPTPVPTPPPAPTPTPI"
BASE COUNT         1919 a         3940 c         3752 g         1825 t
ORIGIN      
        1 tggcgagagc gagcagccca gcgcctccgg gctgccccgt gccaataccg gcaaatgcga
       61 gcgccgcggc aacgaagccc atctgggcga cggccgcttg gcgacgaatc acgcgtcctc
      121 tccaatctcg atctcatggg caccgtccca caggacacgc cggccgacct cgcgcaggcg
      181 gtcgcgccac ccttcatcag gcagcagcgt ggcgaagtga ttgccggcca gctggcccag
      241 cttcgaggag aacatgcagg cgccgtaggg gaagaggatc tcgcactcgg acaggtttcc
      301 ggtgtagatc agcagctccc ccggggacgg atgcgaggtg tggtgctcgt agccgacgcc
      361 ggtctgcagg tcgcccagcg ggatccatgt cccctcgccg ctccagcggc aatggatcaa
      421 ctgcgagcgc atcggtagca ggcgtcgcac cgcctcgacc gttcgcggcg cctcggcttc
      481 ccatcgggca gtaaagttca ggtcgcctac ccggatcgtc aggttcggca tgcgcggagg
      541 atagcagtgg ccgatctccg tcccaccatc tcgacccacg tcctcgacct cgagctgggc
      601 gtcccggcgg cagggctgcc ggtggcgctc ttccggctcg agcaggacgg ccgtccggag
      661 ctgatcaccg agcttgcgac cgatgcagat ggccgcatac cctcgctgct gccgggcgag
      721 ttggaggtcg gcgactacca gctcgtcttc gacctggcgg cctatgcggc gactctcgat
      781 cgagacgccg atttcttcga gaacctggcg gtcggcatcc ggatcgacga cgcggagcgc
      841 agctaccacg tgccgctcct gctttccccc ttcgggatct cggcctatcg cggcacctga
      901 tgggctacct ctcgatcgac gatctgaacg acctgtcagc ggacgcattc gccgatgagg
      961 tggctcccct gttcgagggc gctggcgggt tcctgcggcg cctcgcagcg gctcggccct
     1021 tcgactctta ccactcgttc ctggcgacgg cccgcgaggt ggcccgaacg atgcccgagg
     1081 cggaccagat cgagctgctg aacgcgcatc ccacactggg cggcgattcc gcgttggtct
     1141 cagcagcgtc attcgacgag cagggctacg ggtcgccgga agaggatggg cagcccgtcc
     1201 tcgaagagct ggccatgctg aacgaggtgt acgaggggcg attcgggttt cggtacgtgg
     1261 tcttcgtggc aggtcggccg ccgtcggcga tggggccgct gatcgaggcc gcgatgcgca
     1321 acgatcgtgc ggcggagctg gcccgtggcc tcgatgaggt aatcgaaatc gccggcgacc
     1381 ggcttggccg catgcgcggc gactacctcg accctgagga agggtcctga tgcaatacga
     1441 gatccactat ggcaaggccg atgtcaaggt gtaccgcacc tacgggccgc ccttgcggga
     1501 ggtcaggcag atccccgagt cgccattcgg cggccgcgag aacaccctga tggcggccga
     1561 gctcgaggtg acggtgcacg gcaccgcgtt ccttgacgcg tacacccgtg gcgacaaccg
     1621 gcgggtggtg gcgaccgaca cgatgaagaa cttcatgcac acggcctccc tggcatcgaa
     1681 ggcgcccacc ctcgagggct ggctccacca cgttgggtcg gcattcctgg cgacctaccc
     1741 ggacatggaa cacctcacga tgcacgggcg cgagctgcca ttccccgcag cgctggtccc
     1801 ggctgacgat ggagacggtt tcgccgcctc cgaggtcctc ttcgcccgcg accgcaacga
     1861 ccgctccacg gcgtccctcg agctggagcg ccacgaggac ggtataagaa tcgccggcca
     1921 ccgctgcgga cgaacggagc ttcagctgat caagatcact ggcagcgcct tcgcggactt
     1981 cgcgcgcgac gagcacacca cgctccccga acgccccgat cgcccgctct acgtctggtc
     2041 cgacatcggg tggcggtaca cggacgccct ggacgccctc ggcggcgacc ctggccgata
     2101 cgtggcgggc gagcaggtcg ccgacctggc ggcatcggtc ttccacgggt tcgtcagcct
     2161 atcgatccag cacctcgtga atgagatcgg gattcgaatg ctggaacgct ggccgcagct
     2221 ggccgaggtc tcgttcgacg catccaatcg actctgggat accggggtgc agagcgaggc
     2281 cgacccccgg gtcaagacct acgccgaccc gcgccccccg ttcggaaaga tcgggctggt
     2341 cctgcgccgc gacgactgaa tctagcctgg ctccagctgc gggaagtcga tcgccgggca
     2401 acggtccatc accaccagca gtccggcagc cgccgcccgc tccgcggcct cctcgtccca
     2461 gacacccagc tgcagccaga cggccctcgc gccgatctcg atcgcctcgt ccacgtgcgc
     2521 tcctgcgcgg tgggatgcgc ggaagatgtc gaccacttcg atggggctgc cggcgggccg
     2581 ttgagccgct gcggtgcgca ggtccgggac cgtggcgagg ccatggatcg tcgtctcgcg
     2641 cgggtagacg gggatcacct cgaacccgaa tcgcatcaac gagcgcgtga cgccgaagct
     2701 ggctcgggtg gggtccggcg aggctccgac gacagcccag acacggaatt cgctgaggat
     2761 ccttcgggcg acctcaaagt cgttccagcg cttcgattgc acggtcatcg gttgcgggcc
     2821 agttcctgga gggctgcctt gatgcgcagg tcgaggccgt cctcgcctcc ggccaccgcc
     2881 ccggcggcgc gtcgagcctc cgaggcggta tagcccagcg actccagcgc cgcgacgacg
     2941 tccgagtcgg tgctcgctcc cccgccggct gcgatcccgg cagcgtggat cttctccttc
     3001 agctccagga tgatgcgcag ggccgtcttg cgtcccaccc cgctgaccga ggtgagcagg
     3061 tccgcgtcgt cagtgacgat cgccaactgg agcttggtca cggcatgggc gctggtgatg
     3121 gcgagcgcca ggcgcggccc gacgccggtc acggtcatga gcagctcgaa gaaggcaagc
     3181 tcctccggcg aggcgaagcc gaagagcgcc tgctggtcct cgcgcacgag atggtgcacg
     3241 aagatgcgcg cctcgctacc cagccgaagc tgggcgagca gagacggacc gaccagcacc
     3301 cgatagccga cgccacccag ctccaccagc acgaattcct ggccgacgtg cgtgaccggc
     3361 ccgcgcagcg agccgatcat ccgatcacgg cgaggcgtgc gctggtcatg ccgcgatacg
     3421 cgcagccgat tgccaccgcc agcgcatcgg tcgcgtcgtc ggccacgcca tcgagggcaa
     3481 tccccagcag gcgagcgacc atggacgcta cctgggcctt gtcggcggcg ccgttgccgc
     3541 agaccgcctg cttcacttcg ttcggcgtcg gctcgtcgac cagcagtccg gcttcggcgg
     3601 cggcgagcag ggcaacgcca cgcgcctgcc cgacggccat ggcggtctgc acgttgcgct
     3661 ggaagaagag gcgctcgacc ccgactcgat gcggatggtg cagggtgaag agctcggtca
     3721 gggcactgtg cagggaacgc agccgatccg catcggtctc ggcgctctcc gtgcggatga
     3781 ctccggcgtc gatcaatcga agccgaccgt cgtcgagggc caccaccccg aatccgcagc
     3841 gggcggtgcc tgggtcgatg ccgagcgcga tcaccgcagc atcgtagcgt cgcgcggggt
     3901 caggcgaccg atgcctccag ctgctccatc agcgcatcgg gcaggtcgaa gttggcgtag
     3961 acgttctgca cgtcctccag ctcctcgagg cgctcgacga agcccagcac ctggcgggcg
     4021 gccttctcgt cggagatctc gaccttggtg gtcggctcca tcgagacttc cgccgaggtc
     4081 gcggggtggc cagcgtcggc cagcgacgag cgcacgcgtt caaggtcggc gggctcagtc
     4141 atgaccagga tcacgccgcc ctcgggagtg tcgacgtcgg tggcccctgc atcgatggcc
     4201 gccagcgtca cctcgtcagg gtcactcccc ttgagcggga tgctcagcag gccgcgctgc
     4261 tggaactgcc atgcgaccgg cgtcagcgag cctcccacct tggtgaagat gctgcggacc
     4321 tccgaggcgg tgcggttccg gttgtcggtc atcacggcca cgatcaccgc cacgttggcc
     4381 gggccgagcc cctcgtaggt gatctcctcg tattgcaccg cgtcggcacc ggcgccggtg
     4441 gctcgctcga ttgcacgctt gatgttctcc atcggcatgc tgttgtcgcg cgccttgtcg
     4501 atcgccatcc gcagccgata gttgccatcg acgtcgccac caccctggcg cgcggcagtg
     4561 atgatctcgc gcgtcagctt cgtgaacagc gctccgcgcc gggcgtcgtt gacacccttc
     4621 tgccgcttga tctgtgacca tttgctgtgg ccggacatcg gtcctccggt cggtgtgggg
     4681 agcaccgatt gtagcaatgc aacagcggcc tccctaccat gggcccgatg gtgccgttcc
     4741 tcgatcgggc cgagaacgcc ggcgcgctgc gttacctggc gcacggtcga tccgatgccg
     4801 aggggttttt cggacccccg ccggcggatc tcgaccggcg acacctgggc acgcatccga
     4861 ccgtcgtcga tcggctctgg gaggagctca accaggcgct cccggccgac gctcgctggc
     4921 tcgtcttcga cgggccggcc ctcgtccatc cgggcggatc gatcctggcg gccgcgatgg
     4981 gtacgcagta cgcactgcgg ctgttgactg acgatcgcgc cgccgcgatc gccgccggtg
     5041 ccgaggtcgt ccatcacttc cagaccgtcg gcacgacgct tgacctgccc gccatgtttg
     5101 ggccggagtg ggtgttcggc ctttgggatg aacgcgagcc ggattggctc cgggcgagct
     5161 acgatgccat cgggcctgcg gcctgaggtt ggctatcaca ccacccgctt gcgataccgg
     5221 cagcccgcgc cgcctcccta taattggcct tcatctcggc ttgaagattc gcacgaggcg
     5281 gattcggcct cggctgctgc gacccgcgca caaggagcac cgatgaagcc gaccagtcct
     5341 gatcggatcc ggaacgtggc cctcatctcc cacggtggcg ccgggaagac gagtctcgcc
     5401 gaggcgatgc tcttcgacgc cggcgccatt gcgcgactgg gcagcgtcga cgccggatcc
     5461 gcagcgctcg atcacgaccc tgatgagcag aagcggcggc agagcatcaa cctcgcgatc
     5521 gggaccctcg aggccgacga tgcccgcatc accctggtcg acacccccgg ctatgccgac
     5581 ttccaggccg atgtcatcga ggccctggcg gcggtggacg ccgcgatcct ggtggtcgac
     5641 gcctccgcgg gggtcgaggt cggcaccgag gaggtctggc ggctggccga ggagcgcaat
     5701 ctgccccgct tcatcttcgt caacaagatg gaccgcgaga acgccaacta cgatgccgtg
     5761 ctggacgggc tgaaggcgaa gttcggcccc aagatcgcgc ccgtctacct gccgatcggc
     5821 tccgccgagt cgtttcgtgg ctatatcgac gtcatcgagc agcacgccaa cgtgtacgag
     5881 agcggcaacc cgagcgaggt gccgatcccc gacgacatgc gagccggcga ggaggcccgc
     5941 cgcgaggccc tggtcgaggc tgcggccgag gcgagcgacg agctgatgac ccgctacctg
     6001 gacggcgaga cgatcagcga cgaggagatc gagacggccg tccacaaggg aacccgcgag
     6061 ggcagcgtgg tgcccgtctt cgtggggagc gcactgaaga acatcggcgt ccgcgagctg
     6121 gcatccatga tcgccaagca catcccctcg gcggccgagg tcggcgcccg gaccacgagc
     6181 gacggcaagc gcatcgagcc cgacccggac gcccctctcg tggcacaggt cttcaagacc
     6241 acggcggacc ctttcgtggg ccggctcact tacttccgca tcgtggccgg caccctccat
     6301 gcccagggcc acctgtggaa tgcgacgcgc aaagccgagg agcggatcgg caacatcctt
     6361 ggcctgctgg gcaaggagca ggagaacatg ccccaggcgg gtccgggcga catcgttgcg
     6421 gtcgccaagc tggcgagcac ccagatcggt gacaccctcg tggcggaccg caaccaggcg
     6481 gtcagcctcc cgccgctcga gttcccgtcg ccgagcctgc aggtcagcat cgagcctgag
     6541 agcaaggcgg acctcgacaa gctcggccag gctctctcgc gcatgctcga ggaggagccg
     6601 tgcgcccggg tgcaccgcga ggagggcacc ggcgagacga tcctgaccgc gatgggcgac
     6661 gcccacgtgg atgtcctggt cgagcggctg aagcgcaagt tcggggcatc ggttcgcacc
     6721 ggcagcccgc acgtgccgta ccgggagacc attcggaagc cagcaaagat cgacaaccgc
     6781 ttcaagcgac agaccggcgg ccatgggcag ttcggccacg tggtgatcga gttcgagccg
     6841 ctgccgtccg gatcgggctt ccagttcggc gacaagatcg tcggcggcgt ggttcccagg
     6901 cagtacatcc ccgcggtcga gaaggggctt cgtgagaccc tcgccgaggg ggtgctggcc
     6961 ggctacccgg tcgtggacct cagcgctcgc cttgtggatg gcagctacca caccgtggac
     7021 agctcggaga tggccttcaa ggtggccgcc agccaggccc tcaggcgcgc cttccccgaa
     7081 gccgacccga ccctgctgga gccgatcctg gaggtcgagg tgaccgtccc ggatgcctac
     7141 atgggcgacg tgatgggaca gatcaccgcc aagcgcgggc acgtgctggg catggactca
     7201 tccgacggga tgcagcacct tcgcgcccag gtgccgcagg ccgagatgtt ccactacgcc
     7261 accgagctgc gcagcatcac ccaggggagg ggccggttca gctggaagct cgaccactat
     7321 gccgaggtgc cgagcacgat atctgaccgc gtcatcgccg accaccaggc caaggtgggt
     7381 gccggcgaga agcattgacg cagcgctgtc caacttcgtg cgacgttccc cgacgtcgcg
     7441 ctgacgtagc tcgaggcgct ggctatggct gtcgaaacct gttacttcgt cacgcggatg
     7501 cgcttcccta cgcgtttccc aacacgaatc tccggctggt caccgggcgc aaacgtgggg
     7561 atgtcaggac tggcgactac ttcgccatta agccgcacgc cgccctgacg aacaagattc
     7621 cgtacttgcg tgttcgagcc agccaagccg gacatcgtca tgatctgaat caaggagagc
     7681 tgcccgtcgg gcgctgcgag atcgatggtc gcaatatcgg tgattccctc gcctcggccg
     7741 atcgcgcgct cccattcctc cgctgctgat tcagcggctc cctcgccgtg ccagcggctc
     7801 acgatttcgc gcgccaagcg ctgcttcgcg gccttggggt ggatttcacc tgcggagagg
     7861 gcggctacga ggctatctgc ctcagcgttg gtccatcgcg tgacgaggtg cgcccagttg
     7921 tccatgagtt catcagggat gctcatggtc ttcgcgtact gttcgtacgg agcatcggtc
     7981 aagccaatcg cgttgccaag cgacttgctc atcttctgga cgccatcggt gccgacaagc
     8041 aattcgacgg tcatgatctg ctgcggcgct tggccatggt cgcggagcag atcgcgccca
     8101 acgagcaggt tgaaggtttg atcagtgcca ccgagttcga cgtcggcctg taccgcgaca
     8161 ctgtcgtacg cctggagcag cggatacagc gtctcatgca tgctgatggg gatcccatcc
     8221 ttcaggcgct tggcgaacgt gtcgcgctca attattcgcg cgagcgtgaa atcgctcagt
     8281 agcttgaata cgtctccgag tccgaagcgc ccgaaccatt cactctgcca gcgaacctct
     8341 gtccgttcag gatccacgat cagcccgaat tgggcgagat aggtctcagc attggcgcgc
     8401 acttggtccg cggtgagcat cgggcgcgtc gaattacgct cggaaggatc accgatctgg
     8461 gcggtcgtat cgccaaccac caacaccggc agatggccca agtcctggaa agcccggagg
     8521 cgttcgaatg gcacgccatg gccaatgtgc aggtcggggc tagttgggtc gacaccgaac
     8581 ttgactcgca atggtcggtc gcccgcgagg agtaggcgtc gcagctcgtc atgttcgacg
     8641 acttgcgcaa cccgattgct aaggagcgcg tcgacggctg cttcgcgctt atcgatcttc
     8701 acaggctgtg gagtataagg cgcgtccaac ggctttccgg agtgcccggt cgctccgtgc
     8761 taggcttggc ctcaatggac ggacggcccc caaggcgggc ccggggccgc tcttcagggg
     8821 ggccgccggc gtctcgctcc cccagctcag gccccggtca ccgggttgcg ccactgcccg
     8881 cgctgatcct cggcgccttc gtcgtgctgg ccggcgccct gttcatcggc gtcgtggccg
     8941 tcttcgcatc gtacgccagc ggcctgccgg atccgtccca gctcgcgcac ttcgagctga
     9001 gtcagggatc gagcgtcatg tccgccgatg gggtcgagct cgccaccttc gccgccgagc
     9061 agcggcgcgt cctcacctac gacaagatcc cgcaggtgat ggccgacgcc caggtggcgg
     9121 ccgaggatca gacgttctgg tccaacccgt gcatcgactt caaggggatc gtgcgtgcgg
     9181 cgctgcagaa cttctcggcc tcgcagacgg tctccggcgc ctcgaccatc tgccagcagc
     9241 tggttcgcat ccggctcttc gacgcggacc tgctggccaa ccccgatcgg cgctgggagc
     9301 ggaagatcaa ggaggcgctc ctcgccctgc aggtcggaga cgcgtatccg ggcaccgagg
     9361 gcaagcagaa gttgttggag atgtacctca accaggtcta ctacggcaac aacgcctacg
     9421 gcatctgggc cgccgcaaac cgctatttcg gcaaggacat cacttcgtcg gcgcccgaga
     9481 accagctgag cgtcggagag gctgcactgc tggcggggct cgtgcgggcg ccctctgcac
     9541 tggatccgac ccaggtggcc gtcccgtcca cggatgccgc cggcaacacg atcctggtcg
     9601 tcccgccgga tgccgacgcg atccgggtcc agggctacgt cctgaacggc atggtcaaga
     9661 ccggcgccat caccccggcc gagcgggacc aggcagccgc ggagcagatc gtgctggtgc
     9721 cgccgcagag ccggcagtac aaggctcctc acttcgtcta cgccgtgcgc cgcgccgcgg
     9781 ccgacctgct gggcaacgag gacctgctcg atcgcggcgg cctcgtgatc agcaccacgc
     9841 tcaattacaa cggctaccag gtgagcgccg agaagtgggc gggcgtggcc tacgaccttg
     9901 accggctgag cgaccaggag ctgaccgcga agtacggcga ggccgcgttg gcatggatca
     9961 agcagctgca gggccgcaac atcaacaacg acgcgttggt gacgatcaac taccgaactg
    10021 gagccgtgat ggcctacgtt ggctccgcca actactacgg ggaggcgacc gcggcccacc
    10081 agccgcagta cgacgtggtg ggccaggcct atcggcagtc cggttctgcc ttcaagccga
    10141 tcacctacgc caccggcttc gagcgcggca cgatcacgcc ggcgaccatg ctcatggacg
    10201 tcaaggggat catcgccgag ggctactcag tccctaacgc cagcggcaac gagcgtggcc
    10261 cggtccgcgt gcgcgacgcg ctcaagtact cgtggaacat cccggtcgcc aaggcccagc
    10321 agctgatcgg cacccagaac gtggtcgaca tggccaagcg cctcgggctc tcatgggacc
    10381 cgcagcagaa ggacgtggct gtgccatccc tcacgttggg caccatcggc gtgcacatgc
    10441 tcgacctggc cggggcatac ggcgccatcg ccaacggcgg cacgctctcg aagccgttcc
    10501 tgatcgagaa gatcaccgat cgcgatggaa cggtcatcta cgaccacggc gcggatgcac
    10561 cgaagccgga gcgggtcctg agcgaggatg ccgcctacct ggtgaccgac atcctggccg
    10621 acaacaccaa ccccgccacc aacatcatct ggggtcagcg cttccagctg agcaccgatg
    10681 cgggtcgccg cccggcaacg ctgaagaccg gcaccaccaa cgacttccgc gacctgcagg
    10741 ccttcggcta cctggcaccc gacaaggacc cgaaggtcga cgagggcgcg atcatcacgg
    10801 gggtttgggt cggcaacagc gacttctcgg cgatcaagga cgtcttcgcg gccgatggcc
    10861 cgaccttcat ctggcacgac tacatggccg aggtgagcgc ggccaacaag ctgccgtcgc
    10921 gcgacttccc gcgccctgcg gacatcgtgt cgctcaaggt tgatgctctg actggaatgc
    10981 tgccgggcga cttcaccacc gagacggtga cggaggactt tgccgccgcg caccagccga
    11041 ccgagcgtga cacgctgcac cgcaagctgg ccatcgagga ggccaccggc aagatctggc
    11101 aaccgggatg tggcgaccca acagctgcgt cggcggctcc aggcgcttcg ccgggctcgc
    11161 aggcgcccga ggagaaggtc tacctcgacc tcgtcgacta cgaggcctcg catccgacct
    11221 gggaggaggc caaccggacc tggatcgagc gctacatcgg caaggaggac caggtccgcc
    11281 ggtcgccggc accggcattc gacgccccgc tcgccccgat cgagaagtgc acccccggcg
    11341 agattcccac ctcgacacca tggcccagcc cgtctccgac cccgtcaccc acgcccgtcc
    11401 cgaccccccc ccccgcaccg actccgacgc cgatag
//
LOCUS       DATCQR010000265         2514 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_61646, whole genome shotgun sequence.
ACCESSION   DATCQR010000265 DATCQR010000000
VERSION     DATCQR010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2514
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61646"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(103..1272)
                     /locus_tag="VIN32_08655"
     CDS             complement(103..1272)
                     /locus_tag="VIN32_08655"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HEY8922685.1"
                     /translation="MTGRLRVGIFAGGRSAEHEVSVSSADAVLRAIDRDRFEPYLVYI
                     DREGRWHLPAGPAPLLGEEPLAQLLGAASPAEQTSLLRAHADMPLTVVDDRAELAPRA
                     ILRSLAEAIDVAFLALHGPFGEDGTLQGFLEMAGIPYTGAGVMASGVAMDKVVFKDLM
                     RGHGLPVVDYAWFRRAAWRRAPERVLSDIAEAVGERSVVKPARLGSSVGMTLVHGPGE
                     LADALDEAFRYDSKVIVEAYVAGARELECGVLGNDDPLVFEPGEVISHHEWYDYEAKY
                     VPGLADVVPAAQVDRELAARVMELALAAYRAVDCAGLARVDFLVPPGAVYLSEMNTLP
                     GFTATSMYPKQAELAGLAFPALIGRLIDLGLESAREGRTPGNGDLPGNGDTTGAP"
     gene            complement(1269..2222)
                     /gene="murB"
                     /locus_tag="VIN32_08660"
     CDS             complement(1269..2222)
                     /gene="murB"
                     /locus_tag="VIN32_08660"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEY8922686.1"
                     /translation="MAPINAIELQRLDALAAERGIALVADAAMAPLTTLRVGGTADRL
                     LEVHSREELLAALGLARDSKAPWFVLGNGSDLVVADAGMRGLVIRNRARNVAIDGQHL
                     QADAGAPMALLVRRCTAGGLAGLEFGTSIPGTLGGAVWADAGAHGREMRDVVVAVEAW
                     DPADGSVGSLDAAACRFAYRESRFKHSREVIVAATLALHRDGPDAIARRVAENQAQRV
                     ATQPLADQNAGSVFRNPPGDHAGRLIDAAGLKGLRVGTAGVSALHANFIVTDRGGRAA
                     DVRALGDRVRTEVGAQFGIDLAYEIEFVGEWASASGDEGAE"
     gene            complement(2228..>2514)
                     /locus_tag="VIN32_08665"
     CDS             complement(2228..>2514)
                     /locus_tag="VIN32_08665"
                     /inference="COORDINATES: protein motif:HMM:NF016027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY8922687.1"
                     /translation="VASILVPYPHAAGHQQANADWLAGRGAAVVVPDAELDGDRLVAE
                     ATALLDDEQRRRMGQAAKELARPDAARLLAEELLAMGEGRPLPSLTGTAG"
BASE COUNT          358 a          955 c          865 g          336 t
ORIGIN      
        1 gaccgggggc cggcagcacg cgccggaccc gcctgcgcag cggcgcgcgg ggacgcttgg
       61 catgagccga cgggcgccgc cgtgtcgtcg tcgagcggcg cgtcatggcg ccccggttgt
      121 gtcgccgttg cccggcagat cgccgttgcc aggcgttcgt ccctctcgag ctgattccag
      181 cccgaggtcg atcagccgtc cgatcagcgc cgggaaggcg aggcccgcca gctcggcctg
      241 cttggggtac atgctggtcg ccgtgaagcc cggcagcgtg ttcatctccg acagatagac
      301 cgcgccgggc gggaccagga agtcgacgcg cgccagaccg gcgcagtcca cggcccgata
      361 ggcggccagc gccagctcca tgacgcgcgc cgccagttcc cgatcgacct gcgccgcagg
      421 cacgacatcg gccagtccgg ggacgtactt ggcctcgtag tcgtaccact catggtggct
      481 gatcacctcg cccggctcaa agacgagcgg gtcgtcgttg ccgagcaccc cgcactccag
      541 ctcccgggcg cctgccacgt acgcctcgac gatcaccttg ctgtcatagc ggaacgcctc
      601 gtcaagggcg tcggcaagct cgccggggcc gtggaccagg gtcatcccga cgctgctccc
      661 aagccgagcg ggcttgacca cggaacgctc cccgactgcc tccgcgatgt ccgacagcac
      721 ccgctccggc gcgcgacgcc aggcggcgcg acggaaccag gcgtagtcga ccaccggcag
      781 gccatggcca cgcatcaggt ccttgaagac gaccttgtcc atggccaccc cgctcgccat
      841 cacgccggcg ccggtgtacg ggatgccggc catctcgagg aacccctgca gcgtgccgtc
      901 ctcgccgaac ggcccgtgca gcgccaggaa tgccacgtcg atcgcctcgg ccagggagcg
      961 gaggatggcc cgcggcgcga gctcggcgcg atcgtccacg acggtgagcg gcatgtccgc
     1021 atgagcgcgc agcaggctcg tctgctctgc cggcgaggcg gctcccagca gctgtgccag
     1081 cggttcctcg ccgagcaacg gcgccgggcc ggcgggcaga tgccagcgcc cttcgcggtc
     1141 gatgtacacc aggtacggct cgaatcggtc gcgatcgatc gcccgcagaa ccgcgtcggc
     1201 cgagctgacg ctcacctcgt gctctgccga tcggccaccc gcaaagatgc cgacccggag
     1261 gcgcccggtc attcagcacc ctcgtcgccg gaggcggagg cccattcgcc gacgaactcg
     1321 atctcgtagg cgagatcgat cccgaattgc gcgccgacct cggtgcggac ccggtcaccc
     1381 aacgctcgca cgtcggccgc ccgaccgccg cgatcggtca cgatgaaatt ggcatgcagg
     1441 gcgctgacgc cggcggtgcc gacgcgcagc cccttcaggc cggccgcgtc gatgaggcgt
     1501 cctgcatggt cgcccggcgg gttgcggaag acgctccccg cgttctggtc ggccagcggc
     1561 tgggtggcca cgcgctgcgc ctggttctcc gccactcggc gagcgatcgc gtccggcccg
     1621 tcgcggtgga gggcgagggt cgccgccacg atcacctccc ggctgtgctt gaagcgagac
     1681 tcgcgatagg cgaagcgaca ggcggccgca tccaggctcc cgacgctgcc gtccgcggga
     1741 tcccaggcct ccaccgccac gaccacgtcc cgcatctcgc ggccatgcgc gccggcgtca
     1801 gcccagaccg cgccgcccag ggtgcccgga atgctcgtgc cgaactccag gccggccagg
     1861 ccgccggcag tgcaacgacg gacgaggagc gccatcggcg cgcccgcatc tgcctgcagg
     1921 tgttgcccgt cgatcgcgac gttcctcgcc cgattgcgaa tcacgaggcc gcgcatgccg
     1981 gcgtcggcaa ccaccaggtc gctgccgttg ccgaggacga accagggagc cttcgagtcg
     2041 cgcgccaggc cgagcgccgc cagcagctcc tcgcgagagt ggacctcgag caggcggtcg
     2101 gccgttcccc caacgcgcag cgtggtcagc ggggccatcg cggcgtccgc gacgagggcg
     2161 atcccgcgct cggcggccag ggcgtcgagg cgctgcagct cgatggcgtt gatgggcgcc
     2221 atgagcgtca acccgccgtc ccggtcagcg acggcagggg acgcccctcc cccatggcca
     2281 ggagctcctc ggcgaggagt cgcgccgcat ccggccgagc caactccttc gcggcctgcc
     2341 ccatcctcct ccgctgctcg tcgtcgagca gtgccgttgc ctcggccacc agccggtccc
     2401 cgtcgagctc ggcgtccggt accacgaccg cggctccccg gccggcgagc cagtcggcgt
     2461 tcgcctgctg gtggccggcc gcgtggggat acggcaccag gatggaggcg actc
//
LOCUS       DATCQR010000266         5553 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_138715, whole genome shotgun sequence.
ACCESSION   DATCQR010000266 DATCQR010000000
VERSION     DATCQR010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5553
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138715"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(150..650)
                     /locus_tag="VIN32_08670"
     CDS             complement(150..650)
                     /locus_tag="VIN32_08670"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEY8922688.1"
                     /translation="MTEPGQPTSSADPYQILQVLPSAEQEVLHAAFRALAMKYHPDRD
                     SSARAARRMMELNQAYAMLREPELRAQFDKSRRAARVPFDPASIPPMGGRAGAPPPQT
                     RRTNASSANATSTKLADGRYAGWTLKDLAGHDPDYLKWLARHSSGLRYRKEISLILGS
                     KAVAGG"
     gene            complement(643..1761)
                     /locus_tag="VIN32_08675"
     CDS             complement(643..1761)
                     /locus_tag="VIN32_08675"
                     /inference="COORDINATES: protein motif:HMM:NF015736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HEY8922689.1"
                     /translation="MIEDPTSDPQADPLADPLADLKPAAVDWRLEGEAGALAPRSALD
                     VLTNLNERIASGKVDEFQPIGTGFVPLDKTIGGGLRPGELMLIGGAQGAGKTTMALQM
                     ARNMAASGQATALYVCFEHEEEYLIQRLISMESALGQLPKKTGAVKIQDVKKEVMQSY
                     AAAVEAGQRAKLGTNPRLRPSLERIARYGSNLFLMRGTATGSTIDNLRSLVAEHRGTE
                     GKRLVLFVDYLQKVPVIPEPTMEEEKVTRIVSGLKDIALSLEIPVVAIVAADREGLKA
                     KRLRNHHLRGSSAINYESDIILILNDKYHIVAKVNIEFNPYQAQRYRDWVVATVEKNR
                     GGQDNIDLEYEKLFEFSCFDPTGRVTAEKLIEERLFND"
     gene            complement(2391..4328)
                     /gene="ftsH"
                     /locus_tag="VIN32_08680"
     CDS             complement(2391..4328)
                     /gene="ftsH"
                     /locus_tag="VIN32_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEY8922690.1"
                     /translation="MRNSVVLVVLAVFGVALLWTYVVQPPSTPPYTYGKLLQDAAKGV
                     VTSVEQDGTRLSVVLNGDKENPKAVVVPSSFVNVNAEVCAAAGSNDLGNCPIDYSAVE
                     ESAAGQWLGLLITAFLPVLLIGGFIFFMMRQAQGTNNQAMSFGKSRARMFLGNKTVIT
                     FNDVAGVDEAKQELAEVVEFLKYPEKFNSLGARIPRGVLLVGPPGTGKTLLARAVAGE
                     AGVPFFSISGSEFVEMFVGVGASRVRDLFEQAKRNSPCIVFVDEIDAVGRQRGAGLGG
                     SHDEREQTLNQILVEMDGFDTNTNVIVVAATNRPDVLDPALLRPGRFDRQVVLDRPDI
                     RGRREILNVHIKGKPLDKTVDLDELARRTPGFSGADLANLVNEAAILAARHNKKSVAP
                     SDFNEAIDRVVAGPERRSRLITPEEKTIIAYHEGGHAVVQRVLPKCDPVSKVTIVSRG
                     MALGYTMSLPSEDRYLHSKSEFEDKLAGMLGGHVAEEIVFGDTTTGASNDIEKATGLA
                     RAMVTQYGMSEKLGPLTFGKKDEMIFLGREISEQRNYSDEVAAKIDAEVRELIDRAYQ
                     RAKESLTTHREVLDRLAALLIEKETIESEEFESLFTGVLPPRGSSGPTPRRAGEVDSG
                     EQLPTPQPAPKRRRKPAPQPA"
     gene            complement(4465..5430)
                     /gene="tilS"
                     /locus_tag="VIN32_08685"
     CDS             complement(4465..5430)
                     /gene="tilS"
                     /locus_tag="VIN32_08685"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEY8922691.1"
                     /translation="MVGSDPLRPIIDALTDGAARLEIPDGVAMVLAVSGGPDSTALLY
                     GAAGMASDRGWRLTVAHLDHGLRETSAEEAAAVAANAAGMGLPAEVRRVEVAELATSE
                     HRSLEDAGRQARYRFLEEVAAELGPEALIATAHTADDAAETVLLRLARGSGLRGLRGI
                     PARRGRIVRPLLHARRAVLRAALDAAGIAYLIDPSNANLAHTRNRVRAELLPALERLN
                     PAAVEVLTRFGRLAADDDDLLDALATAELARRRDSPEASIDWHRPPARALGRRVLRLA
                     IGDPAPSAERIEALLDASEGPRGGLSIELGGGRRASVRERRIRLD"
BASE COUNT          829 a         1840 c         1908 g          976 t
ORIGIN      
        1 tcctcgaagg cgatggcgta cacgtgctcc cacgaagggt tggggcgcat cacggtgtcg
       61 ccgatgtcca ggaagacagc acgcaagctc gtcgtcggtt gtgcgtcgct cattctggcc
      121 aagcgtagca agtcaaggcc ccacgtggat cagcccccgg cgacggcctt ggaaccaagg
      181 atgagcgaga tctccttgcg gtagcgcagg cccgaggaat ggcgcgccag ccacttcagg
      241 tagtccgggt cgtggcccgc caggtccttc agcgtccacc cggcgtagcg gccgtccgcc
      301 agcttggtgg aggtggcgtt ggcgctgctg gcattcgtgc gccgcgtctg cggaggaggc
      361 gcccccgcgc ggccgcccat cggtggaatg gaggccgggt cgaacgggac ccgcgcggca
      421 cgccgggact tgtcgaactg tgcccgcagc tctggttcgc ggagcatggc gtaggcctgg
      481 ttcagctcca tcatgcgtcg ggctgcacgg gccgatgaat cgcgatcggg gtggtacttc
      541 atggccagcg cgcggaatgc ggcatgcagc acttcctgct cggccgatgg gaggacctgc
      601 aggatctgat acgggtcggc cgatgacgtc ggctgccccg gctcagtcat tgaagagccg
      661 ctcctcgatc agcttctcag ccgtcactcg cccggtcggg tcgaagcagc tgaactcgaa
      721 cagcttctcg tactcgaggt cgatgttgtc ctgtccgccg cggttcttct cgacggtggc
      781 taccacccag tcgcgatagc gctgggcctg gtacgggttg aactcgatgt tgaccttggc
      841 gacgatgtgg tacttgtcgt tcaggatcag gatgatgtcg ctctcgtagt tgatggcgct
      901 gctgccgcgc aggtggtggt tgcgcaggcg cttggccttc agcccctcgc ggtcggccgc
      961 cacgatggcc accaccggga tctcgagcga cagggcgatg tccttcagac cgctgacgat
     1021 ccgcgtgacc ttctcctcct ccattgtcgg ctccgggatg accgggacct tctggaggta
     1081 gtcgacgaag aggacgaggc gctttccttc cgtgccacgg tgctcggcaa cgagggagcg
     1141 caggttatcg atggtggatc cggtcgcggt gccgcgcatc aggaacaggt tgctgccgta
     1201 gcgggcgatc cgctcgagcg acggtcgcag gcgcgggttg gtgccgagct tggcgcgctg
     1261 ccccgcctcg accgctgcgg cgtagctctg catgacctct ttcttgacgt cctggatctt
     1321 cacggcgccc gtcttcttcg gcagttggcc gagcgcggac tccatggaga tgaggcgctg
     1381 gatgaggtac tcctcctcgt gctcgaagca gacgtagagc gcggtcgcct ggcccgatgc
     1441 ggccatgttg cgggccatct gcagcgccat ggtggtcttg ccggcgccct gggcgccgcc
     1501 gatcagcatc agctcgccgg gtcgcagtcc cccaccgatg gtcttgtcga ggggcacgaa
     1561 gccggtgccg atgggctgga actcgtcaac cttgcccgag gcgatccgct cattcaggtt
     1621 ggtcagcacg tccagggccg atcgcggcgc gagcgccccg gcctctccct ccaggcgcca
     1681 gtcgactgcc gccggcttga ggtcggcgag aggatcggcc agaggatcgg cctggggatc
     1741 gctggtcggg tcttcaatca tgggtgccgc ggctcctcac acgagcaatg ccgcgcatgg
     1801 tgctcatgcg caacggaggc gagtatacga ccagccatct tggctccgaa atggcccgaa
     1861 tgggctaccg gcgacccctc ttcggccccg tcccgtcctg cttattaggc ggacttatga
     1921 acgtgcaccg atggggcctc tactgataag cctgataagc ctggcttatc acacggcacc
     1981 gatcgagcct catccgtgca cgaccataag ccacgcttat cgggcgcgct gccgcctctc
     2041 agctcccgtg ctgaatggct gcgcggacta cgaacgacgc ggagttgggt gcaagccgtc
     2101 gtcccgagtc cactgggcgc atatttcgac gtgatggacg acgtatccgg tccttcgtag
     2161 tccatcgcga catatgtcgc gaggacggcc gtcacgcagg ctgatccgcc cgacctgatt
     2221 actcacagtt atcgagatgc accgatccgg cccgtcccgc gtcgtgtgat aaccgtggct
     2281 tatcgcacgg ccccgatcga gtgccttcgg tgcacgacga ttacccacgg ttatcaggcg
     2341 cgcagacgcg cagggcgcgc gcagacgcgc agcgagcgat cgacgcgagg ctaggccggt
     2401 tggggtgctg gcttccttcg acgcttggga gcgggctgcg gggtgggaag ctgctcgcct
     2461 gagtcgacct ctcccgcgcg tcgcggggtt gggcccgagc tgcctcgagg cgggagaacg
     2521 ccggtgaaga gcgactcgaa ctcctcgctc tcgatcgtct ccttctcgat cagcagggcg
     2581 gccaatcggt cgagcacctc gcggtgcgtg gtgagcgact ccttggcgcg ctggtaggcg
     2641 cgatcgatga gctcgcgaac ctcggcgtcg atcttggcgg cgacctcgtc ggagtagttg
     2701 cgctgctcgc tgatctcgcg gccgaggaag atcatctcgt ccttcttgcc gaaggtcagc
     2761 ggaccgagct tctcgctcat gccgtactgg gtcaccatcg cgcgggccag gccggtcgcc
     2821 ttctcgatgt cgttgctggc gccggtggtc gtgtcgccga agacgatctc ctcggccacg
     2881 tgaccgccca gcatgcctgc gagcttgtcc tcgaactccg acttggagtg gaggtatcgg
     2941 tcctccgacg gcaggctcat ggtgtagccc agcgccatcc cgcggctcac gatcgtcacc
     3001 ttgctgacgg gatcgcactt cggcagcacg cgctgcacca ctgcgtgccc gccctcgtgg
     3061 taggcgatga tcgtcttctc ctcgggggtg atcaggcggc tgcgccgctc cgggccggcc
     3121 actacccggt caatcgcctc gttgaagtcg ctcggtgcga ccgacttctt gttgtggcgc
     3181 gcggccagga tggctgcctc gttgacgagg ttggcgaggt ccgcccccga gaagccgggc
     3241 gttcggcgcg ccagctcgtc gaggtcgacc gtcttgtcga ggggctttcc cttgatgtgg
     3301 acgttcagga tctcccgtcg tcccctgatg tcaggccgat cgagcacgac ctggcggtcg
     3361 aagcggcccg ggcgcaggag cgccgggtcg aggacatcgg gtcggttggt ggctgccacg
     3421 acgatcacgt tggtgttcgt gtcgaagccg tccatctcga ccaggatctg gttgagggtc
     3481 tgctcgcgct cgtcgtgcga gccgcccagc ccggcgccgc gctggcgtcc gaccgcgtcg
     3541 atctcgtcca cgaacacgat gcagggcgag ttgcgcttgg cctgctcgaa gaggtcacgc
     3601 acgcgagagg cgccgacccc cacgaacatc tccacgaact ccgagccgga gatggagaag
     3661 aagggaactc ccgcctcgcc ggcgaccgcg cgggcgagca gggtcttgcc ggttccaggc
     3721 gggccgacca gcagcacgcc gcgcggaatc cgcgcaccga gcgagttgaa cttctcgggg
     3781 tacttgagga actcgaccac ctcggccagc tcctgcttcg cctcgtccac cccggccacg
     3841 tcgttgaagg tgatgaccgt cttgttgccc aggaacatgc gcgcccggct cttgccgaag
     3901 ctcatcgcct ggttattggt cccctgggcc tgccgcatca tgaagaagat gaagccgccg
     3961 atcagcagga cgggcaggaa cgcggtgatc aggaggccga gccactgccc ggcagcgctc
     4021 tcctcgaccg ccgagtagtc gatcgggcag ttgcccaggt cgttgctgcc ggcggcggcg
     4081 cagacctcgg cgttgacgtt cacgaacgaa gatgggacca cgacggcctt gggattctcc
     4141 ttgtcgccat tgaggacgac gctcaggcgc gtgccgtcct gctcgacgga agtgacgacg
     4201 cccttcgccg cgtcctgcag cagcttgccg taggtatagg gcggggtgga ggggggctgg
     4261 acgacgtagg tccacagcag cgccacgcca aacacggcga ggacgaccag gacaacgctg
     4321 ttacgcatga gtcggctgct caacgtggct ctctctcagt ctcaggtgcg ggggatcgct
     4381 ctgcgcctgc gcgcaggccg ggcaagtata ggtgcgaccc ggcaacgcca acaaacgggc
     4441 catctgggcc atgaccgatg gctgtcagtc caggcgaatg cgcctttccc tgaccgatgc
     4501 ccgacggccg ccgcccagct cgatgctgag gccgccacgc ggcccttcgg aggcgtccag
     4561 gagcgcctcg attcgttcgg cggagggcgc cggatcgccg atcgccaggc gcagcacgcg
     4621 acgccccagc gctcgtgcag ggggcctgtg ccagtcgatg gacgcctcgg ggctgtcgcg
     4681 tcggcgcgcc agctcggctg tggcgagggc gtccagcagg tcgtcgtcgt ccgctgccag
     4741 gcgcccaaac cgtgtcagca cctcgaccgc ggcagggttc agccgctcga gggccggcag
     4801 gagctcggcg cggacccggt tgcgcgtgtg ggcaaggttc gcgttggagg gatcgatgag
     4861 gtaggcgatg cccgctgcgt cgagggccgc gcgcaggacc gcacggcggg cgtgcagcag
     4921 cggccggacg atgcgtccgc gccgcgccgg gatgccacgc aggcctctaa gcccgctgcc
     4981 acgtgccagg cgcagcagga ccgtctcagc cgcgtcatcg gcggtgtgag cggtggcgat
     5041 cagcgcctcc ggtccgagct ccgctgcaac ctcctcgagg aagcggtagc gggcctgccg
     5101 accggcatct tccagcgacc ggtgttcgga ggtggcgagc tctgccacct cgactcgccg
     5161 cacctctgcc ggcagcccca tgccggccgc gttggcggcc accgcggcgg cctcctcagc
     5221 ggaggtctcc ctcagtccgt gatcgaggtg ggcgacggtc aggcgccagc cgcggtcgct
     5281 tgccatgccg gccgccccgt acagcagcgc cgtggagtcc gggccgccgg agacagcgag
     5341 caccatcgcg acgccatctg ggatctccag gcgggccgcg ccgtcggtca gcgcgtcaat
     5401 gatcggccgg agcggatcgc taccaaccac attggtagaa ccacgcccat cgggccttga
     5461 ccctccgctc gaggcctgca gacgtcttgc ggcaccaatg gcattggtag ggcgtcgcgc
     5521 gggcggagtc atcggcgcag ttgtaccagc cac
//
LOCUS       DATCQR010000267        12475 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_170248, whole genome shotgun sequence.
ACCESSION   DATCQR010000267 DATCQR010000000
VERSION     DATCQR010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 12475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12475
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170248"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..311)
                     /locus_tag="VIN32_08690"
     CDS             complement(<1..311)
                     /locus_tag="VIN32_08690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8922692.1"
                     /translation="MIGADRRRRALIAIGVWLAVGIGFGLRSADPAHADPPGAAARAQ
                     LMADAAVAAEDGLVRLGSELDRAREHARRGTALTASGAAPAPELTAAAAIVAGASDEA
                     DA"
     gene            318..1358
                     /gene="add"
                     /locus_tag="VIN32_08695"
     CDS             318..1358
                     /gene="add"
                     /locus_tag="VIN32_08695"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF013155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HEY8922693.1"
                     /translation="MDLDRMRMLPKAELHLHLDGSMRPQTAVELAAEGGRELRLEDAI
                     SRLVGPVHCANQAELLTFFDLPISLLQTPGSLRRVTAELVDSLADDGVTYAEIRWAPR
                     LHLERDMSVPVVIEAVARGVAEAASRLGPRTPFIGLIVTAMRSHPPAANVELAKAAAA
                     FGPPVVGFDLAGPEAEYPAPPHAAAFLAAASGGLDLTAHAGEVPGPERIREALAFGVR
                     RIAHGVTAAEDPDTMALLRDRDVTLDLCPTSNVQTGIVAELAVHPLAALHRAGVSVTL
                     STDDRTVANTSLTEEMARSAAAMQLTADELAVIAVNGFRRAFGPRPVVDLMMRAAEAA
                     WSAWLAEARVIT"
     gene            complement(1351..2205)
                     /locus_tag="VIN32_08700"
     CDS             complement(1351..2205)
                     /locus_tag="VIN32_08700"
                     /inference="COORDINATES: protein motif:HMM:TIGR01460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HEY8922694.1"
                     /translation="MDRSGEPALRLVIFDLDGVMYRGREPVPGAAALVAALRDRGLLI
                     RFATNNSMATRATYVARLLEMGVPVELAEIVTSTSATIDHLHAHLPEVRSVMAVGAPG
                     MLEELRAAGFAATAAGDAVDASYDGGPLEGSFHAVIAGLDPSFDFRRLAAATTAVRAG
                     AHFIATNADLRYPTPTGFLPGAGSIVAALRAASGEEPLVIGKPEPGIFSAILERAGVR
                     PEEAVAIGDNPDADMVAAHRAGMRSILVLTGVADAALAATLDGERRPDQVARDPSEVA
                     PLLGVGFR"
     gene            2377..3567
                     /locus_tag="VIN32_08705"
     CDS             2377..3567
                     /locus_tag="VIN32_08705"
                     /inference="COORDINATES: protein motif:HMM:NF014647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY8922695.1"
                     /translation="MRKQRWLGVSAALVTLALVLAACQSNGSSAGPSNTGSATASASS
                     AASAAPQSDLKIGVVTDAAGINDKGFNEYSYKGAQQGATDIGATAPAVVVPKDQTEYA
                     TAIQSMVDEKLDVIVTVGFNLAGPTIAAAKANPDIWFIGVDQQVGPGKDICVDETGAV
                     DPTYACKGDATALLPKYVQLIFKEDQAGYLAGMVAASVSKSGVVAAVGGLTTCGPCVR
                     YIQGYELGAKSIKSDITVISAYVTKSFDPVKSFGDQPGGKLFAQNLMASNSTLDVLFA
                     VGGLTGNGVLDAACDKGIWGIGVDVDQFVSYAADSKCILTSAEKHLASAVDQALKAAD
                     AGSLTPGPLTFDATNDGVGVSDFHDNASAVPTDLQAKLDDALAAMKAGSLTTCPTDCG
                     APPK"
     gene            3659..5242
                     /locus_tag="VIN32_08710"
     CDS             3659..5242
                     /locus_tag="VIN32_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012190588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8922696.1"
                     /translation="MSVPMVEPPDVVVTPAIEMRGITKRYPGVVANDRIDLVVRPGEI
                     HALLGENGAGKTTLMNVLYGLTTPDEGEILLNGNPVRISGPADAISRGIDMVHQHFML
                     VPVLTVAENILLGLEPMSGPIFIDREAARRRIVELGRQFGFEIDPDARVSSLSVGWQQ
                     RVEILKALYRNTRILVLDEPTAVLTPQETEEIFAVLRNLAAEGHSIVFISHKLYEVLE
                     IADRITVIRRGRVVGERIPAETNEEDLAELMVGRGVELTIDRGTSHPGPVVLRVDALR
                     VKDDRGNEVVRGVSLEIRAGEILGIAGVAGNGQDELVEALYGLRRPSGGSVTLRDANV
                     TGRSPRGMNEAGVGYVPADRQRFGLVLPFPVADNIVLTSYYRAPFARGILRNDDAILA
                     AAVQGIDEFDVRTRSADASVRTLSGGNQQKVVVAREFGRDLSLLVLDQPTRGLDVGSI
                     EFIHRQVIAKRDAGTAVLLVSAELDEVLEMADRIAVMYRGEIVAVVDGRTADKNDVGL
                     LMATGGQGRPEAGAASVTS"
     gene            5239..6396
                     /locus_tag="VIN32_08715"
     CDS             5239..6396
                     /locus_tag="VIN32_08715"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8922697.1"
                     /translation="MTGESRSPRATLTRFVRAAWLVGALPVVSIALALVVGAVVILAS
                     ELLLPNHAFDPFLPISSYLALLDGSFGQGSAIVNTLVSTTPLLLGGLAVAIGFKAGLF
                     NIGAQGQFLMGALGAVAVGVAVNQAPPLVAIPVALAGGMLAGAAWGFIPGFLKAVSGA
                     HEVVTTIMLNYVAGSVLAWAVTGPLAVPQSPTPVTYAVGNAAYPILIGRNGHLGILVA
                     AAAVVVIWWLLYRTTLGFEIRTVGASPDAARYAGMRPRLLIILTMSMAGLLAGTAGAG
                     VILGINHQMNSTFATTVGFDAIAVALLARSNPLAVPFAALLFGAMRAGSGLMQIRAGI
                     PVELIDVIQAMILLFLVAGPALQRLFRMTAVHSDLGGPETVVPPDPGQVSL"
     gene            6396..7475
                     /locus_tag="VIN32_08720"
     CDS             6396..7475
                     /locus_tag="VIN32_08720"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8922698.1"
                     /translation="MDPFINTLYGIPVLGLVFQLVGYIIQVIPPIAPLIMRAGAPLAL
                     GAMCGVMCERSGVVNIAIEGTMLASAFTGWVVAVALGPYLGTSSSLPFGITPTLLIAL
                     AAALLVALLVSGIHAWLSITVKADQIISGTIINIAAFGVTGYLNTILAPLSPTSGGNF
                     SAFIPPKAVTDLPVVGWIFAMFLNQGPIAMSVLVLVVVFQILLFRSRWGLRSRAVGEH
                     PRAVETAGIDVIRLRYRNVMVGGVFAGLAGAFLSMEATNQFQQGMSGGRGFIALAAMI
                     VGRWTPIGAFGAAILFSTFAALGQVIGFNPPSGSLGEVVAKLPPQTWDALPYLVTIII
                     LAGIVGRSTPPAADGQPYEREQAGA"
     gene            complement(7492..8247)
                     /locus_tag="VIN32_08725"
     CDS             complement(7492..8247)
                     /locus_tag="VIN32_08725"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEY8922699.1"
                     /translation="MRIAVLADLHANLPAVRAVLDDVLALGCDTMWCVGDVVGRGPHP
                     SEVVDELRALAVPTIQGNWDEAAALHRKHPGVVWPDTEAETAGQLSLEWTIRQLSEDQ
                     RSWLRQLPVTSRFAMDERSVLLFHGSPMRQNEYLWSDRPSRVFARVVSDQGDDLLCFG
                     NTHEAFHRLVGKSHFVAAGSVGCGRLDDMSAQYAVIDVAGPDVMVNFRSVPYDHFSVE
                     ADMRAAGLSPMLLRAPPSSHLALDAESEHLVEA"
     gene            complement(8313..9404)
                     /locus_tag="VIN32_08730"
     CDS             complement(8313..9404)
                     /locus_tag="VIN32_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006563532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HEY8922700.1"
                     /translation="MVDQLTDENIMAALSQVKDPEIGKDLVSLGMVKAIEITGSTVDL
                     TIELTTPACPLKAKIESDIVDGLAVLGATTVRVDWASNVKRSFGGPAADLIPGVKNTI
                     AVASGKGGVGKTTVAVNLAVSLARDGARVGLLDADITGPNVPMMMGTSGSHVTGVGSR
                     VNPVVAHGVQMMSIEYFLTGGAPVIWRGPLIHSAIQQFLRDVEWGELDYLIVDLPPGT
                     GDAALSLSQLIPLSGAVVVTTPQEVSLLDARKAIAMFNKVNVRTIGVVENMSGFICPD
                     CGTEHDIFGTGGANSLSRELGLEVLARIPLEPSVRTGGDAGAPITSTQHPSSDTSVAA
                     AALRELARTVAGRISVIAEPFAGARARAS"
     gene            complement(9510..10376)
                     /locus_tag="VIN32_08735"
     CDS             complement(9510..10376)
                     /locus_tag="VIN32_08735"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HEY8922701.1"
                     /translation="MSSIWRPVRERNPARSLMVVVAHPGDEAFGFGGAIAHAAASGAY
                     TVVVCATRGAFDRRLLDPAPAPGGRNRSYQKPLIWRNLDTVREDELRRSVGLLGVRVL
                     RMLDYSEEGLSKVDFDELVGRIVQPIRMHRPEVILTFGPDGVSGDPDHEAVGRATKAA
                     FERAAEPLAYEDDLEEDQVAWRAAKLYQLVVPAAALRPLGDRAPDGYGSPDGTDTLTL
                     ELGELAQVKLSAIGRHVSQTGSAGPFHDWRPEVRDAFLATEHYRLAGSNLPVPTGGGG
                     PPTESGLWDGLA"
     gene            complement(10395..11468)
                     /locus_tag="VIN32_08740"
     CDS             complement(10395..11468)
                     /locus_tag="VIN32_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012526622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY8922702.1"
                     /translation="MPRPRLDPRIPLAELHVHLGAAVTPAIMWGIAHAQGIRLPTKDY
                     WAFRELITVGRRRRSFDAYLDLFHWTELIQSSPIAVERSVYEVIGGAYRKSNITRIEL
                     RFNPMKRNRGGEQDLDHIIAAAVRGMDRAVLEYPQVRAGLILCLDRAFSHDLNEIIAA
                     KAISWHSRGVAAVDIAGPVDPSFRFIDYRDLFDECRRAGLGVTVHAGETGGPEEVREA
                     IEALEPSRIGHGVKSAYDPTVMGMLRERGIVLEVCPSSNLTTRVVRNVAEMRFILRSL
                     IDNAVPFTLSTDGPEMLRSHLRDELALLLRSEMMTLEEIHQAIQTAHAASFLNDEQPL
                     PGGAAGVSAAGDGHAPIALEVEV"
     gene            11555..11953
                     /locus_tag="VIN32_08745"
     CDS             11555..11953
                     /locus_tag="VIN32_08745"
                     /inference="COORDINATES: protein motif:HMM:NF024183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEY8922703.1"
                     /translation="MELIRDVINFIATDLGDFGATLAPYAFIGMVGLYLLWVVIGYLR
                     VSQVGIQAESTTLPALPLPRAPNGAIDVPRGVPYCPNDGLRYPAGARFCTRCEADLLA
                     SCANCGATIRAADESCYRCGTHATLVAASD"
     gene            complement(12049..>12475)
                     /locus_tag="VIN32_08750"
     CDS             complement(12049..>12475)
                     /locus_tag="VIN32_08750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEY8922704.1"
                     /translation="NSVAADVLLRLALLAGHPDHDRRARSILRAAGPALERHPAQFGR
                     MLAAADRALGEPIDAVIVGDAIDRAAVALRRAAATPFQPDLVIAPLSPGAAIAAWPVF
                     EGKTARDGLATAYVCRGYACDEPTQDPERVLSQVGRLSD"
BASE COUNT         1897 a         4329 c         4265 g         1984 t
ORIGIN      
        1 gcgtctgcct cgtcgctggc gccagcgacg atcgcggcgg cggcggtcag ctccggagct
       61 ggcgccgcac cggaggcggt cagcgccgtg ccgcgccgcg cgtgctcacg ggcacgatcg
      121 agctcgcttc cgaggcgaac cagaccgtct tccgcggcga cggcagcgtc agccatcaac
      181 tgcgcgcgag ccgccgcgcc gggcgggtcg gcatgggccg gatcggcgga acgcaggccg
      241 aagccgatcc cgaccgccag ccagacgccg atggcgatca gcgctcggcg ccggcgatcg
      301 gcacctatca tctgtcggtg gacctcgatc gcatgaggat gctgccgaag gcggagttgc
      361 acctgcatct ggacggctcg atgcggccgc agaccgcggt ggagctcgct gccgagggcg
      421 gccgggagct caggctggaa gacgccatct cgcggctggt cgggccggtc cactgcgcga
      481 accaggcgga gttgctcacc ttcttcgacc tgcccatctc gctcctccag acgccaggct
      541 cactcaggcg ggtcaccgcc gagctggtcg actcgctggc cgatgacgga gtcacgtacg
      601 ccgagatcag gtgggcgccg cgcctccacc tggagcggga catgtccgtc ccggtggtga
      661 tcgaggcggt ggcccgtggc gtcgccgagg cggcctccag gctcggtcca cgcacgccgt
      721 tcatcggcct gatcgtcacc gccatgcgct cacaccctcc ggccgccaac gtcgagctgg
      781 cgaaggcggc ggcggccttc gggccgccag tggtcgggtt cgatctggcg ggcccggagg
      841 cggagtatcc ggcacccccg cacgcggccg ccttcctggc agcggcctcg ggcggcctgg
      901 acctgacggc gcacgccggt gaggtgcccg gaccggagcg gatccgcgag gcgctcgcct
      961 tcggagtgcg gcgcatcgcc cacggcgtga cggccgccga ggatccggac acgatggcgc
     1021 tgcttcgcga ccgtgacgtg accctggacc tgtgtcccac cagcaacgtg cagacgggca
     1081 tcgttgccga gctggcagtc cacccgctgg ctgccctgca ccgcgccgga gtcagcgtga
     1141 ccctctccac cgacgatcgc accgtcgcga atacgtcgct caccgaggag atggcgcgca
     1201 gcgcggccgc catgcagctc accgccgacg agcttgcagt catcgcggtc aacggcttcc
     1261 ggcgagcgtt tggtccccgc cccgtggtcg acctgatgat gcgtgcggca gaggccgcat
     1321 ggtcggcgtg gctggcggag gctcgcgtca tcacctgaat ccgacgccca gcagcggggc
     1381 cacctcggac gggtcgcggg caacctgatc cgggcggcgc tccccgtcga gggtcgcggc
     1441 cagcgccgcg tccgcgacgc cggtgaggac aaggatcgac ctcatgcctg cgcgatgggc
     1501 ggcgaccatg tcggcgtcgg ggttgtcgcc gatggccaca gcttcctcag gtcgcacgcc
     1561 ggcccgctcc aggatcgcgc tgaagatccc gggctccggc ttcccgatga ccaggggctc
     1621 ttccccgctc gcggcgcgga gggcagccac gatcgatccg gcgccgggca ggaagccggt
     1681 cggggtgggg tagcgcaggt ccgcgttcgt ggcgatgaag tgggcgccgg cgcggaccgc
     1741 agtcgtggcg gctgccaggc gccgaaagtc gaatgacggg tcgagcccgg caatcacggc
     1801 gtggaacgac ccctcgagcg gtccgccgtc gtacgaggca tcgaccgcgt cgcccgccgc
     1861 cgtggcagcg aaccccgccg cgcgcagctc ctcgagcatg ccgggcgcgc cgaccgccat
     1921 gacgctgcga acctcgggca ggtgggcgtg cagatggtcg atcgtggcag aggtcgaggt
     1981 cacgatctcg gccagctcga ctggaactcc catctccaac aggcgggcca cgtaggtcgc
     2041 tcgcgtcgcc atcgagttgt tggtggcaaa gcgaatgaga aggccccggt cgcggagagc
     2101 cgcgacgagc gcggcggcgc ccgggaccgg ctcccgaccg cgatacatga cgccgtcgag
     2161 gtcgaagatc acgaggcgca gagcgggctc tccggatcgg tccatgccgg acggatgata
     2221 gggatgccga gcgcacaagg cacttgagcg gctgcctgac cgcgcagtat gatcgcgcgg
     2281 attcgcgccg gcctcgccct cgggcacagg acgcgaccga cccccgcgat cgctcgcggc
     2341 gcctgccatc agtgagtgag gggagaagga gtatccatgc ggaagcagcg atggctcggc
     2401 gtgagcgccg ccctcgtaac cttggcgctt gtgctggcgg cctgccagtc gaacggctcg
     2461 tcagccggac catccaacac ggggtcggcg actgcgtcag cgtcgtcggc tgcgtcggcc
     2521 gcgccacaga gcgacctgaa gatcggcgtc gtgactgacg ctgccgggat caacgacaag
     2581 ggattcaacg agtactccta caagggggct cagcagggcg ccacggacat cggcgccacg
     2641 gccccggccg tcgtcgtccc gaaggaccag accgagtacg ccacggcgat tcagtccatg
     2701 gtcgacgaga agctcgacgt catcgtgacg gtcggcttca acctcgccgg cccgaccatc
     2761 gccgcggcca aggcgaatcc cgacatctgg ttcatcggcg ttgaccagca ggtcggcccg
     2821 ggcaaggaca tctgcgtcga cgaaacgggc gcagtggatc cgacctacgc ctgcaagggc
     2881 gacgcgacag cgctcctgcc gaagtacgtc cagctcatct tcaaggagga ccaggcgggc
     2941 tacctcgccg gcatggtggc ggcgtccgtc agcaagagcg gcgtcgttgc cgccgtaggc
     3001 ggcctgacga cctgcggacc ctgcgttcgc tacatccagg gctacgagct cggcgccaag
     3061 tccatcaagt ccgatatcac cgtgatatcg gcctatgtga ccaagtcgtt cgatccggtc
     3121 aagtcgttcg gcgaccagcc gggcggcaag ctcttcgccc agaacctgat ggcctcgaac
     3181 tcgacccttg acgtcctgtt cgcggtgggc ggcctcaccg gcaacggcgt cctcgacgcc
     3241 gcgtgcgaca agggcatctg ggggatcggc gtcgacgtcg accagttcgt ctcgtacgcc
     3301 gccgattcga agtgcatcct gaccagcgcc gagaagcatc ttgcatcggc cgtggaccag
     3361 gccctcaagg cagccgacgc aggttcgctg acccccggtc ctctgacctt cgatgcgacg
     3421 aacgacgggg tcggcgtctc cgacttccac gataatgcca gcgccgtgcc gaccgacctc
     3481 caggcgaagc tggacgacgc tctcgcggcg atgaaggcag gctcgctgac gacctgcccg
     3541 acggactgcg gcgccccgcc aaagtgattc gcggatcgcg gtgattcgcg gatctggctg
     3601 aaaccgaggg cgggtcggca tcgatgctga cccgccctct ccactccatc cgctcagcat
     3661 gagcgtgccg atggtcgagc cacctgacgt ggtcgtcacc ccagcgatcg agatgcgggg
     3721 tatcacgaag cgctatccgg gcgtcgtcgc gaacgatcgc attgatctcg tcgttcggcc
     3781 cggcgagatc catgcgctgc tcggggagaa cggcgccgga aagacgaccc tgatgaacgt
     3841 gctctacggg ctcaccacgc ccgacgaagg cgagatcctg ctgaacggca atccggtcag
     3901 gatcagcgga ccggccgatg cgatcagccg cggcatcgac atggtccatc agcacttcat
     3961 gctcgttcca gtgctgacgg tggccgagaa catcctcctc ggcctggagc cgatgtccgg
     4021 gccgatcttc atcgaccgcg aggcggcgcg gcgccgcatt gtcgagctcg gccggcaatt
     4081 tggcttcgag atcgatcctg acgcgcgagt tagcagcctg tcggtcggct ggcagcagcg
     4141 ggtagagatc ctcaaggccc tgtaccgcaa tacccgcatc cttgtcctcg acgaaccgac
     4201 ggccgttctg accccgcaag agaccgagga gatcttcgcc gtcctgcgaa acctcgccgc
     4261 ggaagggcac agcatcgtct tcatcagcca caagctgtac gaggtccttg agatcgctga
     4321 tcggatcacc gtgattcgcc gcggccgagt cgtcggggag cgcattccag ccgaaacgaa
     4381 cgaggaggac ctggccgagc tcatggtggg tcgcggggtc gaactcacga tcgaccgagg
     4441 gacgtcccat cccggacctg tcgtccttcg cgtcgatgcc ctccgcgtca aggacgaccg
     4501 cgggaacgag gtcgtccggg gggtcagcct ggagatccgc gctggcgaga tcctcggcat
     4561 cgccggcgtc gccggcaacg gccaggatga gctcgtcgag gccctctacg gcctgcgccg
     4621 accatcaggc ggctcggtga cgcttcgcga cgccaacgtg accggacggt ctccgcgcgg
     4681 catgaatgag gccggtgtgg gatacgtccc cgcggatcgc cagcggttcg gcctcgtcct
     4741 gccgtttcct gtcgccgaca acatcgtcct cacgtcctac tatcgcgccc cctttgcccg
     4801 ggggatcctg cgaaacgacg acgcgatcct ggcggccgcc gtccagggca ttgacgaatt
     4861 cgacgtgcgc acccgctcgg ctgatgccag cgtccgcacc ctttcgggcg ggaaccagca
     4921 gaaagtcgtg gtcgcgcgcg agttcgggcg ggacctctcg cttctcgtcc tcgaccagcc
     4981 gacgcgtggg ctggacgtcg gcagcatcga gttcatccac aggcaggtca tcgccaagcg
     5041 tgacgccggc acggcggtgc tcctcgtgtc ggccgagctc gacgaagtcc tggagatggc
     5101 agaccggatc gccgtcatgt accggggcga gatcgtcgcc gtcgtggacg gtcggaccgc
     5161 cgacaagaat gacgttggcc tgctgatggc cacgggcggc caggggcggc cggaggctgg
     5221 cgcagccagc gtgacgtcgt gaccggcgag tcgcgatcac cacgggccac cctgacccgg
     5281 ttcgttcggg cagcctggct ggtaggcgcc ctcccggtcg tgtcgatcgc ccttgcactc
     5341 gtcgtcggcg ccgtggtcat cctcgcgtcg gagctgctcc ttcccaatca cgccttcgat
     5401 cctttcctcc cgatcagctc gtatctggcg ctgctcgacg gctccttcgg gcaagggtcg
     5461 gcgatcgtca acaccctcgt ctcaaccacg ccgctcctcc tcggcggcct cgccgtcgcg
     5521 atcgggttca aggcggggct cttcaacatc ggcgcacagg gacagttcct catgggagcg
     5581 ctgggcgccg tcgctgtcgg cgtggcggtc aatcaggccc cgccgctcgt cgccattcca
     5641 gtcgcactcg ccggtggcat gctcgccggg gccgcctggg gattcatccc cgggttcctc
     5701 aaggccgtct caggggcgca tgaggtggtg acgacgatca tgctcaacta cgtcgccggt
     5761 tcggtgctgg cctgggcggt gacgggaccc ctggccgttc cgcaatcgcc gacgccagtg
     5821 acctacgccg ttggcaacgc tgcctacccg atcctgatcg gccggaacgg gcatctcggg
     5881 atcctggtcg cggctgccgc ggtcgttgtc atatggtggc tgctctaccg cacgacgctc
     5941 ggcttcgaga tccgcaccgt cggggcaagc ccggacgcgg cgcgctatgc ggggatgcgg
     6001 ccgcggctgc tgatcatcct gacgatgagc atggcaggct tgctcgccgg caccgctggc
     6061 gccggtgtca tcctcggcat caaccaccag atgaactcga cgttcgcgac gacggtcggc
     6121 ttcgacgcga tcgccgtcgc tctccttgcc cgttccaatc cgcttgccgt cccattcgcg
     6181 gcgctcctgt tcggggcgat gcgggcaggc tcagggctga tgcagatcag ggccggcatt
     6241 ccggtcgagc tgatcgacgt cattcaggcc atgatcctgc tgttcctcgt ggccggtccc
     6301 gcactccagc gcttgttccg aatgacggcc gtccacagcg atctgggcgg cccggagact
     6361 gtcgtaccgc cagatcctgg ccaggtgtcg ctctgatgga cccgttcatc aacacgctgt
     6421 acgggatccc ggtcctgggg ctggtgttcc agctcgtcgg ctacatcatc caggtcattc
     6481 cgccgattgc gccgctcatc atgcgcgccg gggcgccgct cgcccttgga gccatgtgcg
     6541 gtgtcatgtg cgagcgatcc ggcgtcgtca acatcgcgat cgagggaacg atgctggcga
     6601 gcgcgttcac aggctgggtc gtcgccgtcg cgctcgggcc gtaccttggg acaagctcca
     6661 gcctcccctt cggcatcaca ccgacgctcc tgatcgcact cgcggcagcc ttgttggtgg
     6721 cgcttctcgt ctcgggcatc catgcctggc tgtcgatcac ggtgaaggcg gaccagatca
     6781 tcagcggcac gatcatcaac atcgcggcct tcggcgtgac cggctacctg aacacgatcc
     6841 tggcgccact ctcgccgacc agcggcggca acttcagtgc gttcatccct cccaaggccg
     6901 tcaccgacct tccggtcgtg ggctggatct tcgcgatgtt cctgaaccag ggtccgatcg
     6961 cgatgtcggt gctcgttctg gtcgtggtct tccagatcct tctgttccgg tcgcgctggg
     7021 ggcttcggtc ccgggctgtc ggagaacacc cacgggccgt cgagaccgcc ggcatcgacg
     7081 tcatccgcct gcggtatcgc aacgtcatgg tcggcggggt gttcgccggc ctggcgggag
     7141 cgttcctcag catggaggcg accaatcagt tccagcaggg catgagcggc ggtcgaggct
     7201 tcatcgcgct ggcggcgatg atcgtcggac ggtggacgcc gatcggcgct ttcggtgcag
     7261 cgatcctgtt ctccaccttc gcggctctcg ggcaggtcat cggcttcaat ccgccgagcg
     7321 gcagcctggg cgaagtggtg gcgaagctcc cgccgcagac gtgggatgcc ctgccatacc
     7381 tggtcacgat catcatcctg gccggcatcg tcggccggtc gaccccgccg gcggcggacg
     7441 gtcagccata tgagcgcgag caggcgggcg cctgagcgtc cgttcccgga atcaggcctc
     7501 gaccagatgc tcggactcgg cgtcgagggc caggtgagac gaaggcggcg cccgaagcag
     7561 catcggcgac aggccggccg cccgcatgtc cgcctcgacg ctgaagtggt cgtaggggac
     7621 cgatcggaag ttcaccatca cgtcgggccc ggcgacgtcg atcacggcgt attgcgcgct
     7681 catgtcatcc agcctcccgc agccgaccga cccggccgcg acgaagtggc tcttgccgac
     7741 cagccgatga aaggcctcgt gcgtattgcc gaagcacagc aggtcgtcgc cctggtcgct
     7801 cacgacgcgg gcaaagacgc ggctcggtcg atcactccac aggtattcgt tctggcgcat
     7861 cggcgaaccg tggaacagga gcaccgaacg ctcatccatt gcgaaccggc tggtgacggg
     7921 cagctgccgc agccaggagc gctggtcctc ggagagctgc ctgatcgtcc actccagcga
     7981 gagctgcccc gcggtctcgg cctccgtgtc tggccagacc acgccgggat gcttcctgtg
     8041 cagcgccgca gcctcgtccc agttgccctg gatggttggc acggccaggg cacgcagctc
     8101 gtccacgacc tcggaagggt gcgggccgcg cccgaccacg tcgccgacgc accacatcgt
     8161 gtcgcagccc agcgcgagca cgtcgtcgag caccgcgcgc acggcgggca ggttggcgtg
     8221 caggtcggcg agcacggcga tccgcatgga gccgattctc accggcagcg gggcggcggc
     8281 aatcccccgc gggcaggact tccgggtgga ggctagctgg cgcgggcgcg ggcgccggcg
     8341 aacggctcgg cgatgacgct gatgcggccg gcgacggtgc gagccagctc acgcagcgcg
     8401 gcggcagcga ccgaggtgtc gctcgagggg tgctgggtcg aggtgatcgg tgcgccggca
     8461 tcgcccccgg ttcgcaccga gggctccagg ggaatgcgcg ccaggacctc gagcccgagc
     8521 tcgcggctca ggctgttggc cccaccggtg ccgaagatgt cgtgctcggt gccgcaatca
     8581 gggcagatga acccgctcat gttctcgacc acgccaatcg tccgcacgtt gaccttgttg
     8641 aacatggcga tcgccttgcg cgcatcgagc aggctcacct cctggggcgt ggtcacgacc
     8701 actgccccgg agagcgggat gagctggctc aggctcagcg cggcgtcgcc cgtgcccggc
     8761 ggcaggtcga cgatcaggta gtcgagctcg ccccattcga cgtcgcgcag gaattgctgg
     8821 atggcggagt gaatgagcgg gccgcgccag atgaccggcg ctccgccggt caggaagtac
     8881 tcgatgctca tcatctgcac cccgtgcgcc accaccgggt tgactcggga accgacgccg
     8941 gtgacgtggg atccgctggt gcccatcatc atcggcacgt tcggccctgt gatatcggca
     9001 tcaaggaggc cgacccgggc gccgtcgcgc gccaggctca cggcgaggtt cacggccacg
     9061 gtcgtcttgc cgacgccgcc cttgcccgag gcgacggcga tcgtgttctt gacccccggg
     9121 atcaggtcag ccgccggccc cccgaacgag cgcttcacgt tgctcgccca gtccacccgc
     9181 acggtcgtcg cacccagcac ggcaaggcca tccacgatgt ccgactcgat cttggccttc
     9241 agggggcagg cgggagtggt gagctcgatc gtgaggtcga ccgtcgagcc ggtgatctcg
     9301 atcgccttga ccatgcccag gcttaccagg tccttcccga tctccgggtc cttgacctgt
     9361 gacagggcag ccatgatgtt ctcgtcggtc agctggtcga ccatggtcaa gagaagttcc
     9421 tttccggcat tgggaggcgt cgctgccgcg agcgtaccag agcgggccgg cgatcgctgc
     9481 tacctatgtt gaggacggtc gggcccaggc taggcgaggc cgtcccacag tccggactcg
     9541 gtgggcggcc cgccgccgcc cgtcggcacc ggcaggtttg acccggcaag ccggtaatgc
     9601 tcggtggcca ggaaggcgtc ccgcacctcg ggccgccagt cgtggaacgg accggcagat
     9661 ccggtctgcg acacgtggcg gccgatggcg ctcagcttga cctgggcgag ctccccgagc
     9721 tcgagcgtga gcgtgtcggt gccatccggc gagccatagc catcgggggc tcggtccccg
     9781 agcggacgca gcgcggcggc cggcacgaca agctggtaca gcttggccgc gcgccaggcc
     9841 acctggtcct cctccaggtc gtcctcgtac gcgagcggtt cagccgcacg ctcgaaggct
     9901 gccttcgtgg cacgccccac cgcctcgtga tcaggatcgc cgctgactcc atcgggccca
     9961 aaggtcagga tcacctccgg ccgatgcatg cggattggct gcacgatccg tccgacaagc
    10021 tcgtcgaagt ccaccttcga gagcccctcc tcggagtagt cgagcatgcg cagcacccgc
    10081 accccgagca ggccgaccga tcggcgcagc tcgtcctcgc gaacggtgtc caggtttcgc
    10141 cagatgagcg gcttctggta ggagcggttc cggccgccgg gcgccggggc tgggtcgagg
    10201 agccgccgat cgaatgcgcc gcgagtcgcg cagaccacga cggtgtaggc acccgacgcc
    10261 gccgcgtggg cgattgcgcc gccgaagcca aacgcctcgt cgcccggatg ggcgaccacg
    10321 accatcaggc tgcgggcagg gttccgttca cggaccggcc gccagatcga ggacatggcc
    10381 gcgagtctat caacctagac ctcgacctcg agggcgatcg gcgcgtggcc atctccggcc
    10441 gcagaaacgc ccgccgcgcc accagggagc ggctgctcgt cgttcaggaa gctcgctgcg
    10501 tgagccgtct ggatcgcctg gtggatctcc tcgagcgtca tcatctcgct ccgcagcagc
    10561 agtgccagct cgtcgcgcag atgcgagcgc agcatctccg gaccatcggt ggacagggtg
    10621 aacggcaccg cgttgtcgat cagcgatcgc aggatgaagc gcatctcggc aacgttgcgc
    10681 accacgcgcg tggtgaggtt ggacgagggg cacacctcga gcacgatgcc gcgttcgcgc
    10741 agcatcccca tgacggtcgg gtcataggcg ctcttgacgc cgtggccgat ccgcgacggc
    10801 tcaagcgcct cgatcgcctc gcgcacctcc tcgggacccc ccgtctcgcc cgcgtgcacc
    10861 gtcacgccca gcccggcgcg ccggcactcg tcgaagaggt cgcgatagtc gatgaatcgg
    10921 aatgacggat cgacaggccc ggcgatgtcg accgccgcca cgccgcggct gtgccagctg
    10981 atcgccttgg cggcgatgat ctcgttcagg tcgtggctga aggcacggtc caggcagagg
    11041 atgaggcccg cgcgaacctg cgggtattcg aggaccgcgc ggtccatgcc ccgcaccgct
    11101 gccgcgatga tgtggtcgag gtcctgctcg ccgccgcggt tgcgcttcat ggggttgaag
    11161 cgcagctcga tgcgggtgat gttgctcttc cggtaggcgc cgccaatgac ctcgtacacg
    11221 ctgcgctcga cggcgatcgg gctcgactgg atcagctcgg tccagtgaaa gaggtcgagg
    11281 taggcgtcga aggagcgacg ccggcgtccg acggtgatca gctcccggaa ggcccagtaa
    11341 tccttggtcg gcagcctgat cccctgggcg tgggcgatcc cccacatgat ggccggcgtt
    11401 accgccgcac cgaggtggac gtggagctcg gcgagcggaa tacgggggtc gagccggggt
    11461 cggggcatct tcctcgcggg gcgacgattt ggccgagtgt agcgcagccc ggcgacccgg
    11521 cgccgtctgg ggccgatgct agaatcgccg cgccatggag ctcatccggg acgtcatcaa
    11581 tttcatcgcc acggaccttg gtgacttcgg cgcgacgctg gcgccgtatg ccttcatcgg
    11641 catggtcggg ctctacctgc tgtgggtcgt catcggctac ctgcgggtct cgcaggtcgg
    11701 catccaggcc gaatcgacga cgctgccggc gctcccgctg ccccgcgccc ccaacggcgc
    11761 gatcgacgtg ccacgcggag tcccctactg cccaaacgac ggcctgcgct accccgccgg
    11821 cgcgcgcttc tgcacccggt gcgaggccga cctgctggcc tcttgcgcga actgcggtgc
    11881 aacgattcgc gccgccgatg agtcctgcta ccgatgcggc acccacgcca cgctcgtggc
    11941 cgcctcggac tgagcgccgc cgcccgactc cttgtccggc gggccggtgc gggactcttc
    12001 gtcggccagc attcctacgc cggcgcctga cggctgcgcg ctatctgatc agtcggacag
    12061 gcgccccacc tgggacagca cgcgctcagg gtcctgcgtc ggctcgtcgc acgcgtagcc
    12121 acgacacacg tacgcggtcg ccaggccatc gcgcgccgtc ttcccctcga acacgggcca
    12181 ggccgcgata gcggcaccgg gcgagagcgg ggcgatcacc aggtccggct ggaatggcgt
    12241 ggcagccgcg cgtctgaggg cgactgccgc ccggtcaatt gcgtcgccga cgatgacggc
    12301 gtcgatcggc tcgcccagtg cgcggtccgc cgcggccagc atccttccga actgggcggg
    12361 gtgccgctcc agcgccgggc ccgcggcccg caggatgctg cgggcgcgac gatcgtgatc
    12421 ggggtggcca gcgagcagcg caaggcgcag caggacgtct gccgcgacgg aattg
//
LOCUS       DATCQR010000268         2268 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_61937, whole genome shotgun sequence.
ACCESSION   DATCQR010000268 DATCQR010000000
VERSION     DATCQR010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2268
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61937"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..871
                     /locus_tag="VIN32_08755"
     CDS             <1..871
                     /locus_tag="VIN32_08755"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEY8922705.1"
                     /translation="ARAEASREHAVESAARRSLAFADFHTHDRFSRDSILSEDKFIRV
                     ALERGLTHVAVTNHNNVEGAIAVRDRVAALGVDDQLTVILGEEVSTSDGEVVGLFLER
                     TIPRGLTADQTADEIHAQGGLVSIPHPFDPIRPSHIRRLPLENLAAAGKIDMIEVFNS
                     RVTFSRHNDQAADFAARHGIVGIACSDSHSAFEVAMSFNALPAFTTADELREGLTENE
                     WHGSRSTILIHLTTRWAVWSNIVRGWLGRGTATAPVLGPEPPKQVEKEPVEAPAPSEL
                     PNAGDPEASDERG"
     gene            858..2000
                     /locus_tag="VIN32_08760"
     CDS             858..2000
                     /locus_tag="VIN32_08760"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEY8922706.1"
                     /translation="MSGDSEPPGERPGHHPTGSHEHGHSLYEPGEFLDAPPDEVIPER
                     EVSLARRLLNVRTIGSIVFGLVLVFLLFRVILNVDFGRAFELMRGANPWMLLAGLVAY
                     YLTFPLRSLRWTLILRRVGTPVSYRAATEILFLSWFVNCIVPAKLGDLYRAYLLRGNF
                     GASISRTVGTIFIERVTDIIVIFALALAAGFWSFRGRNNPQVDALFVAGFVVAVVLVL
                     LVVALRYLGGHLTRVLPERVAELYARFHEGSTGALTPRVLLPVGAITVAVWLLEGARV
                     YFVIHALNLTGMQLGISSSVFVALAASLLTAIPLTPAGIGFVQAGIVYALVLYGVAPE
                     AGTAVALTDFVLSTLSVIVIGGVFYAFSPMIRRAHAGGAATESARA"
BASE COUNT          351 a          792 c          731 g          394 t
ORIGIN      
        1 cgcacgggcc gaggcgagcc gcgagcacgc cgtcgagtcg gccgcccgcc gctcacttgc
       61 cttcgcggac ttccataccc atgaccgctt cagtcgcgac tcgatcctgt cggaggacaa
      121 gttcatccgc gtcgcgctgg agcggggcct gacgcacgtg gccgtgacca accacaacaa
      181 cgtcgagggg gcgatcgcgg ttcgcgaccg ggtggccgct ctgggcgtcg acgatcagct
      241 caccgtgatc ctcggcgagg aggtctccac ctcggacggc gaggtggtgg ggctcttcct
      301 ggagcgcacc atccctcgcg gcctgaccgc cgaccagacg gccgacgaga tccacgccca
      361 gggcgggctg gtctcgatcc cgcatccgtt cgatccgatc cgcccatcgc acatccgtcg
      421 gctgccgctg gagaacctcg cagcggccgg caagatcgac atgatcgaag tgttcaacag
      481 ccgggtgacc ttcagccggc acaacgatca ggccgccgac ttcgcggcgc gacacggcat
      541 cgtgggcatc gcatgcagcg acagccattc cgcgttcgag gtggccatga gcttcaacgc
      601 tctccctgcc ttcacgaccg ccgacgagct tcgagagggg ctcaccgaga acgaatggca
      661 cggcagccga tcgaccatcc tcattcacct cacgacccga tgggccgtgt ggagcaacat
      721 cgtccgcggc tggctcggcc ggggcaccgc cacggcgccg gtccttggcc cggagccgcc
      781 gaagcaggtt gagaaggagc ccgtggaagc accggcaccg tccgagctgc cgaacgcggg
      841 agacccagag gcctcggatg agcggggata gcgagccgcc gggggagcga cccggccacc
      901 atccaaccgg ctcgcacgag cacggccact ccctctacga gccgggcgag ttcctggacg
      961 ccccacccga cgaggtgatc ccggagcgag aagtgagcct tgccaggcgt ctgctgaacg
     1021 tccgcacgat cggctccatc gtcttcggcc tggtcctcgt cttcctcctg ttccgcgtca
     1081 tcctcaacgt cgactttggc cgggcgttcg agctcatgcg cggggcgaat ccctggatgc
     1141 tcctcgccgg tctcgtcgcg tactacctca cattccccct gcgcagcctc aggtggacgc
     1201 tcatccttcg gcgtgtcggc acgcccgtca gctaccgggc cgctaccgag atcctcttcc
     1261 tgtcctggtt cgtgaactgc atcgtgccgg cgaagcttgg ggacctgtac cgcgcgtacc
     1321 tgctgcgagg caacttcggc gcctcgatct cacgaacggt cgggacgatc tttatcgagc
     1381 gcgtcaccga catcatcgtg atcttcgccc tcgcgctcgc ggcgggattc tggagctttc
     1441 gtggacgaaa caatccgcag gttgatgcgc tcttcgtggc ggggttcgtg gtcgcagtcg
     1501 tcctcgtgct actcgtcgtc gcgctgcgct acctcggcgg acacctgacg cgggtgctcc
     1561 ccgaacgggt cgcggagctg tatgcgcggt tccacgaggg cagcactgga gcgctgacgc
     1621 cacgcgtcct gctgccggtc ggcgcgatca cagtggccgt ctggctcctt gagggggccc
     1681 gggtctactt cgtgatccat gccctgaacc tcaccggcat gcagctcggg atcagctcat
     1741 cggtattcgt cgccttggct gcatctctcc tcactgccat cccgctgacg cccgccggaa
     1801 tcggcttcgt ccaggcgggg atcgtgtacg cgctcgtgct gtacggggtg gcgccagagg
     1861 ccggaaccgc ggtggcgctg accgacttcg tgctcagcac gctcagcgtg atcgtgatcg
     1921 gtggggtctt ctacgccttc tcgcccatga ttcggcgcgc gcacgccggc ggggccgcaa
     1981 cggagagcgc gcgggcctga cgtcgcaccg catgccggct gcggtcggta cctttgtccc
     2041 attgagagac cacggcccga ctgcgattct gcggttgctc gcacgcacca cctgggcgat
     2101 tcatgtccgg gtgggcgacg gcacaggacg aaggcgcatg acgctccaga ctccgcggtc
     2161 gttcaacagc tggctgcgag gccagctgaa ggaaaagaag atgagccagc gccagctggc
     2221 cctccagtcg ggtgtcgatc actcgaccat ctcccgcttg atcaaggg
//
LOCUS       DATCQR010000269         9835 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U7258
            k141_200805, whole genome shotgun sequence.
ACCESSION   DATCQR010000269 DATCQR010000000
VERSION     DATCQR010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955072
            Sequence Read Archive: SRR1586324
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 9835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 17:33:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,767
            CDSs (total)                      :: 1,736
            Genes (coding)                    :: 1,729
            CDSs (with protein)               :: 1,729
            Genes (RNA)                       :: 31
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9835
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200805"
                     /isolate="SMAG_U7258"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="Canada: Axel Heiberg Island,Nunavut"
                     /lat_lon="79.42 N 90.76 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            295..969
                     /locus_tag="VIN32_08765"
     CDS             295..969
                     /locus_tag="VIN32_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007918636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY8922707.1"
                     /translation="MHLLLVEDDPKLGRLLTRMLQEERHIVELTASGEEALEIAEANG
                     GIDAMVLDIGLPDIPGTDVARRLRAKGSRLPILMLTARDAVDDRVRGLDAGADDYLVK
                     PFAFSELSARLRALARRVAPDAHDGVILRNGPISLDEVRRLVVVDDVPVELSPREFSL
                     LECLLRHPGQVLTRDQLLDHAWPYGDMLTHNTVDTYVHYLRSKLGSGSARISTVRGIG
                     YRMAEA"
     gene            966..2276
                     /locus_tag="VIN32_08770"
     CDS             966..2276
                     /locus_tag="VIN32_08770"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY8922708.1"
                     /translation="MSDPSVLRQPGDARLLSRVRWRLVAWSAGSTLILLLVLGTALYL
                     SVDASLAASGRSQLEHQAEGLRNYLAKLPPGGLTDSGGEGNESPPIGRAEFGGPGSGT
                     ISIVLDQDGDFMGTKPADTTDLPVRDGVSAALAGATDIRLASVNGAPVRVLSESVEVD
                     GAPHVVQVIQLRAAEERTLTTLLVVLLGGGLAVLALAVAVGFVYAGRALVPIRDSLRR
                     QREFAADASHELRTPLTVVRGSVEYLRRHARQPVAEVGDALNDIESEVDRLTSLVDDL
                     LLLARGDSGAIELRRESVDLAEVAGEALQRLRAQAAARGVQLRLDAAPATVGGDPDRL
                     RQLVAILIDNAIRHSPNGAEVRVGIRGEAHPGISVEDEGPGIRSEDLPHLFDRFWRAA
                     DAPEGGTGLGLSIAAWIAEHHGGRISASNRPGGGARFEVQLPHA"
     gene            2376..2879
                     /locus_tag="VIN32_08775"
     CDS             2376..2879
                     /locus_tag="VIN32_08775"
                     /inference="COORDINATES: protein motif:HMM:NF021538.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2269 family protein"
                     /protein_id="HEY8922709.1"
                     /translation="MPELVPWFLILHVLGAIIAFGPTFSFSVIGGLGGAEPMHGNFAT
                     RISEAITTRRVIPVGLTMPITGIGLIWSVGIDPFARHDRWLLAGIILYTVAITFALVV
                     QLPTVRRIVAMTAGPPPGATPAPPPTGPPPGLMDAVHRVQRGGMLLSGLVVAIAFLMV
                     VKPDLGF"
     gene            complement(2876..3013)
                     /locus_tag="VIN32_08780"
     CDS             complement(2876..3013)
                     /locus_tag="VIN32_08780"
                     /inference="COORDINATES: protein motif:HMM:NF024843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain containing protein"
                     /protein_id="HEY8922710.1"
                     /translation="MYSTNYADKTLSCVDCNQAFTFPATDQQFYAEKEFSEPRRCPTR
                     P"
     gene            complement(3124..3351)
                     /locus_tag="VIN32_08785"
     CDS             complement(3124..3351)
                     /locus_tag="VIN32_08785"
                     /inference="COORDINATES: protein motif:HMM:NF021378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HEY8922711.1"
                     /translation="MFWDRHNDIALWWPMGLGALVCLVIIGVLVWVVVRATSQRHGST
                     ESPEELLRRRFAAGEIDEEEFAKRSEVLRRK"
     gene            complement(3364..4116)
                     /locus_tag="VIN32_08790"
     CDS             complement(3364..4116)
                     /locus_tag="VIN32_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018557484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEY8922712.1"
                     /translation="MTLATGLLRVEQIMGTAITIDLRDDGVPVAALDEAFASLVRADA
                     RYSTYRPDSEVSRLGRGEMVLADCSLELRQVLTLAEQLRVDSDGHFDVRHRADGALDP
                     SGLVKGWAVEEAAWILDQAGARNYAINAGGDIVLRGEPEPGQPWRVGIRHPLLAEAVA
                     HVLELRTGAVATSGTYERGEHISRPRAGDGSRDWLSLTVVGPSLAYADAYATAGFAMG
                     HPGLAWVNRHDGYRAYGMIGNASGVRWIEGSN"
     gene            complement(4113..4592)
                     /locus_tag="VIN32_08795"
     CDS             complement(4113..4592)
                     /locus_tag="VIN32_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003798561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HEY8922713.1"
                     /translation="MPKRGVISALLTAIGVVLLINFKTPSQQGFAAFNRQTVTGTTNT
                     DGTASAGGTPPPTATVPPTAAATPTTKTITGPAISYFFGTAQVQVTLENGKITDVTAV
                     QLPNEGQSAWISQQVAPILRSEVLSAQSAQINLISGATYTSAAYRQSLQAALDQARA"
     gene            complement(4607..5980)
                     /locus_tag="VIN32_08800"
     CDS             complement(4607..5980)
                     /locus_tag="VIN32_08800"
                     /inference="COORDINATES: protein motif:HMM:NF013918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin reductase family protein"
                     /protein_id="HEY8922714.1"
                     /translation="MAAIPTTLARVPMPRTWRLKPIDVAALLVGNGLFIAAMWWRHGG
                     LDQIPTPGGPFIAAGQLTALLGTYTALVQLVLMSRSPWLDQLFGMHRLAAWHRWIGFA
                     TVWLLLGHGAFTTIGYAAIGHTSPIGELWLLLTTFPYVLMATAGMAMLIAVGVTSVRI
                     ARRKLAYETWHGIHLYGYLAIALATAHQLVVGSDFMHDPVARIYWISLYVAVAAMVLT
                     FRVGQPIAISLRHRLRVANVVTEAPGVVSVYLVGCRLEQLAVRAGQYFVWRFLTRDGW
                     WRGHPFSLSAAPNGEYLRLTIKQSGDYTRLLQQLRPGTRVFAEGPYGVLTGARRTRPH
                     LLLIAGGIGIAPLRALLEELPVARGNLTLLYRASDWDDVVFRDEIDHLVEQRGGLVRY
                     LVGRRGTREMPTDPLQARALRRLVPDIGRRDIYLCGSIQMMEAVEESLEILRVPPAQI
                     HTERFSY"
     gene            6245..6424
                     /locus_tag="VIN32_08805"
     CDS             6245..6424
                     /locus_tag="VIN32_08805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8922715.1"
                     /translation="MIIDMTAGPPPETPPGAAPSGPPPALMALVHKVQRGGMFLSVMI
                     AVIVFLMVVKPNLGF"
     gene            complement(6432..7193)
                     /locus_tag="VIN32_08810"
     CDS             complement(6432..7193)
                     /locus_tag="VIN32_08810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8922716.1"
                     /translation="MFDDRRDRLEARLAELAAEVEFPATPPLVALVGERLAQAPPRIG
                     LGRPVSRGLALAMAATVLLAGIAVAFGIGVGGLRLVFGPASFSPIPSMVVGPGLGAPT
                     SLDAARGGVTFTLRVPGLPELGQPDRVYLADPPAGGAVTLLYGERSGFPTDPASGIGL
                     IVTQFRADIGPDVFEKLIEGGVRVMPARVHGEPAWWVAGGDHFFFYRDAQGHIVDSTL
                     RLASPTLIWEEGGVTYRVEGAPSLAEAARVAESLD"
     gene            complement(7186..7773)
                     /locus_tag="VIN32_08815"
     CDS             complement(7186..7773)
                     /locus_tag="VIN32_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018561237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY8922717.1"
                     /translation="MGGVGVAEVEGRPLLEREWIESARRGDASAFEELVRHYQEIAFR
                     TAYLVIGDADEARDAAQDGFVRAHAALGRFREGAEFRPWLLRIVANAARNRRRSASRR
                     ADLALRAFHDAVSGDAAPSPEVLLLADERRRELLAAINGLRVDDRLVIALRWFLDLSE
                     EEMAAVLACPRGTVKSRLSRATARLREAIGEASDV"
     gene            complement(7850..8206)
                     /locus_tag="VIN32_08820"
     CDS             complement(7850..8206)
                     /locus_tag="VIN32_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CxxC-x17-CxxC domain-containing protein"
                     /protein_id="HEY8922718.1"
                     /translation="MYTPSTYSDKILSCVDCNQEFVFAATDQQFYAEKEFSEPRRCAS
                     CRAARKAARGESGGYSSGSSYGSGSGYGGGSSYGGGQREMFTATCDGCGGEARVPFRP
                     TGSKPVYCSNCFSTRR"
     gene            complement(8367..8645)
                     /locus_tag="VIN32_08825"
     CDS             complement(8367..8645)
                     /locus_tag="VIN32_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018334682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8922719.1"
                     /translation="MKGDRVEAVVDTGQGTQTFEIEATRAGRRIEVTTSRGVVEVSEV
                     TRGGTPVRTGRFMSSRLIALVEHPAHEGRDSKVDVQTRHRITKAGDGL"
     gene            8766..9356
                     /locus_tag="VIN32_08830"
     CDS             8766..9356
                     /locus_tag="VIN32_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018638120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase-like oxidoreductase"
                     /protein_id="HEY8922720.1"
                     /translation="MGRARDPGDKASRLPPGQYLTDGFPVLSAGPTPQVPLDTWEFTI
                     TGLLKTPIRWSWQDFIALPSRDWTVDISCVTKWTKLDTIWHGVSLDDILAAVEIEPRA
                     RFVTAHSYGGYTTNLPLTDLLNNQAFVAWQYGGAPLEPAHGGPARLVVPHLYFWKSAK
                     WVRGLQLREDDEPGFWESLGYHNRGDPWREERYSGD"
     gene            9374..>9835
                     /locus_tag="VIN32_08835"
     CDS             9374..>9835
                     /locus_tag="VIN32_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015931645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY8922721.1"
                     /translation="MAEPLAWQISTVSAIRSETPEVSSFTLKLPNWRAHRAGQHYDVR
                     LTAADGYQAQRSYSIASPPSRSGEVDLTIERIADGEVSPYFHDIVEVGDQVEVRGPIG
                     GYFVWEPSLGGPLLLVAGGSGVVPLMAMLRERASADRKPPAVLLYSSRTVDD"
BASE COUNT         1537 a         3402 c         3352 g         1544 t
ORIGIN      
        1 gcatgaatgt ggtgcctgcc cctgccaagg ctccgcctgc gatcgcgagc gcgaggatgg
       61 ccgcggcaat cggcctgatc agcgtgtggt gtgtgtcgtc tggatcgttc atgcccccag
      121 gctggactcc gagtctggga cggtcctgag agcgctaccc cgtcgctccc atgcgattcc
      181 catcaatggc tccaagaatc cggatcatga atgcaccgat ctcccgcgac ccggcccacg
      241 ccgccatctg ggataccctt gcgacgctgc ggcatcccgc actggaatcg accgatgcac
      301 ctgctcctgg ttgaagacga ccccaagctc ggacggctgc tgacccgcat gcttcaggag
      361 gagcgccata tcgtcgagct gaccgcctcc ggcgaggagg cgctcgagat tgccgaggcg
      421 aacggcggga tcgacgccat ggtcctcgac atcggcctgc cggacatccc gggcaccgac
      481 gtcgcccgcc gcctgcgggc gaagggctca cgcctgccga tcctgatgct caccgcccgc
      541 gatgcggtcg acgaccgggt caggggcctg gatgccggag ccgacgacta cctggtcaag
      601 ccattcgcct tctcggagct gtcggcgcgg ctgcgtgcgc tggcccgtcg cgttgccccc
      661 gacgcgcacg atggggtcat cctgcgcaac ggcccgatct cgctcgatga ggtccggcgg
      721 ctggtggtcg tcgacgacgt cccggttgag ctgagtccgc gcgagttcag cctgctcgag
      781 tgcctcctgc gccacccggg acaggtgctg acccgtgacc agctgctcga ccacgcctgg
      841 ccgtacgggg acatgctgac ccacaacacc gtcgacacct acgtccacta cctgcgttcg
      901 aagctcggca gcggatccgc caggatctcg accgtgcggg ggatcggcta ccggatggcg
      961 gaggcgtgag cgacccctcg gtcctgcggc agcccggtga cgcgcgtctc ctgagccggg
     1021 tgcgctggcg actggtggcc tggagcgccg gctcgaccct gatcctgctg ctggtcctgg
     1081 gaacggcgct ctacctcagc gtcgacgcgt cgctggccgc gtcgggtcgc agccagctcg
     1141 agcaccaggc ggaagggttg cggaactacc tggccaagct gccgcctgga gggctgaccg
     1201 attcgggtgg cgaaggcaac gagagccccc ctatcgggcg cgccgagttc ggcgggccag
     1261 gatcggggac gatctcgatc gtcctcgacc aggacgggga cttcatgggg acgaagccgg
     1321 ccgacacgac cgatctcccc gtgcgcgacg gggtgagcgc ggcgctcgcc ggggccaccg
     1381 acatccggct ggcgtcggtc aacggggcgc cggtccgggt tctcagtgaa tcggtcgagg
     1441 tcgatggcgc gccccacgtg gtccaggtga tccagctgcg ggccgccgag gagcgcaccc
     1501 tcacgacact gctggtcgtt ctgctgggcg gcgggctggc agtgctggcg ctggcggtgg
     1561 cagtcggctt cgtctacgcc gggcgcgccc tggtcccaat ccgggattcg cttcgtcgcc
     1621 agcgtgagtt cgccgccgac gccagccacg agctgcgcac cccgctgacg gtcgtgcgcg
     1681 gcagcgtcga atacctccgc cgccacgcca ggcagccggt ggcggaggta ggtgatgcgc
     1741 tcaatgacat cgagagcgag gtggatcggc tcaccagcct cgtcgacgac ctgctcctgc
     1801 tggcgcgcgg cgattccggc gccatcgagc tgcgccgcga atcggtagac cttgccgagg
     1861 tcgccgggga ggcgcttcag cgactgcggg cgcaggcggc cgcgcgcggc gtgcagctcc
     1921 gactcgacgc ggcgccggca acggtggggg gggacccgga ccggctccgc cagctggtcg
     1981 ccatcctgat cgacaacgcc atccgccact ccccgaacgg tgccgaggtg cgcgtcggca
     2041 tccgtggcga agcccatccg ggcatctcgg tcgaggacga ggggcccggg attcggagcg
     2101 aggacctgcc gcatctcttc gaccggttct ggcgcgcggc cgatgcgccg gagggcggca
     2161 ccggccttgg actgtcgatc gccgcctgga tcgccgagca tcacggcggt cgtatcagcg
     2221 cctcgaaccg gccaggcggc ggcgcacgct tcgaggtcca gctaccccac gcctgacgcc
     2281 ctccggccgt gcattcaggt tgcatctggc ctgccggcag cgcccggaga tagactggcg
     2341 gacgtcgttc cggaaacgag cctgcgagga gaccgatgcc cgagctggtg ccgtggttcc
     2401 tgattctgca cgtgctgggg gcgatcatcg ccttcggccc gaccttctcc ttctccgtca
     2461 tcggtggatt gggcggcgca gaaccgatgc atggcaactt cgcaacgcgg atctccgaag
     2521 cgatcaccac ccgccgcgtc atcccggtgg gactgacgat gccgatcacc ggcatcggcc
     2581 tgatctggtc agttggtatc gatccattcg cccgccacga ccgatggctc ctggctggga
     2641 tcatcctcta cacggtggcg atcacctttg cgttggtggt ccagctgccc accgtacggc
     2701 gaatcgtggc catgacggcc ggaccacctc cgggtgcgac accggcgccc cctcccaccg
     2761 gaccgccgcc cggcctgatg gatgccgtgc accgggtgca gcgcggaggc atgctgctga
     2821 gcggcctggt ggtggccatc gccttcctga tggtggtcaa gccggacctc ggcttctagg
     2881 gccgcgtcgg gcagcggcgc ggctcgctga attccttttc cgcgtagaac tgctggtcgg
     2941 tggcggggaa ggtgaatgcc tggttacagt caacgcagct cagggtcttg tcggcatagt
     3001 tggtggaata caaagaaaaa actccttctc gtgcggcttc agcggtcctg tctcgccgta
     3061 aaccgtgagg aggagtgagc gtcgatgccg cgccaccaca cttcagcagg aaagcggcat
     3121 cccttacttt cgccgaagca cttccgaacg cttcgcgaac tcctcctcgt cgatctcccc
     3181 ggccgcgaag cgacgacgca ggagctcctc gggggattcg gtcgagccat ggcgctggct
     3241 ggtggctcgc accaccaccc ataccagcac gccgatgatc accaggcata ccagtgcgcc
     3301 gaggcccatc ggccaccaca gggcgatgtc gttgtgtcgg tcccagaaca tcctttgcgt
     3361 cccctagttg ctgccttcga tccagcgtac gccactcgcg ttgccaatca tgccgtaggc
     3421 ccgatatccg tcatgccgat tcacccatgc caggccggga tgccccatcg cgaagccggc
     3481 tgtggcatag gcgtcggcat acgccaggct tggcccaacc acggtcagac tgagccagtc
     3541 gcgcgaccca tcgcccgcac gcggacgcga gatgtgctca ccgcgctcat aggtgccgga
     3601 ggtggccacg gccccggtgc gcagctcgag gacgtgcgcc actgcctccg ccagcagggg
     3661 atgccggatg ccgacccgcc acggctgccc cggctcgggc tcgccgcgga gcacgatgtc
     3721 gccgccggca ttgatcgcgt agttccgcgc gccggcctgg tccaggatcc aggctgcctc
     3781 ctcaaccgcc cagcccttca ccaggccaga gggatcgagt gccccgtcgg cgcgatgtcg
     3841 cacgtcgaag tggccgtcgc tgtcgacgcg cagctgctcg gccagcgtga gcacctggcg
     3901 gagctcgagg ctgcagtcgg ccagcaccat ctcgcctcgg ccgagccggc tcacctcgct
     3961 gtccggtcga taggtgctgt accgcgcgtc ggcgcgcacc agggaggcga aggcctcgtc
     4021 gagcgccgcc acgggcacgc catcgtcgcg caggtcgatg gtgatggcgg tgcccatgat
     4081 ctgctccact cgcaggaggc cggtggcgag ggtcacgcgc gcgcctggtc gagcgccgcc
     4141 tggagcgact ggcggtacgc cgcgctggtg taggtggccc cggagatgag gttgatctgc
     4201 gcgctctgcg ccgacagcac ctcggagcgc agtatcggcg cgacctgctg cgagatccag
     4261 gccgactgcc cttcgttcgg gagctggacc gcggtgacgt cggtgatctt cccgttctcg
     4321 agcgtcacct gcacctgcgc cgtcccgaag aagtacgaga tggcggggcc cgtgatggtc
     4381 ttcgtcgtcg gcgtggcggc cgcagtcggc gggaccgtgg cggtcggtgg cggagtgcca
     4441 cctgccgagg cggtcccgtc ggtgttcgtg gtcccggtga cggtttgccg gttgaacgca
     4501 gcgaagccct gctgcgaggg ggtcttgaag ttgatgagca gcacgacgcc gatggcggtg
     4561 agcagtgctg agatgacgcc gcgtttcggc aaggttcggc ctccggtcag tagctgaatc
     4621 gctcggtgtg gatctgcgcg ggcggcacgc ggaggatctc caggctctcc tcgaccgcct
     4681 ccatcatctg gatcgagccg cacaggtaga tgtcgcgccg cccgatatcc gggaccaggc
     4741 gacgcagcgc tcgggcttgc agcggatcgg tcggcatctc gcgcgtgccg cgccgcccca
     4801 cgaggtatcg aaccaggccg ccgcgctgct cgaccaggtg atcgatctcg tcacggaaga
     4861 cgacatcgtc ccagtcgctg gcccggtaca gcagcgtcag gttgccgcgg gccaccggca
     4921 gctcctcgag aagcgctcgc agcggcgcga tcccgatccc gccagcgatg aggagcaggt
     4981 gcggtcgcgt gcggcgcgca ccggtcagta cgccgtaggg gccctccgcg aagacgcgcg
     5041 tcccaggccg cagctgctgg agcagtctcg tgtagtcgcc agactgcttg atcgtcaggc
     5101 gcaggtactc gccgttcggc gcggcggaga gcgagaacgg gtggccgcgc caccagccat
     5161 cgcgcgtcag gaagcgccaa acgaagtatt ggccggctcg gacggccagc tgttcgagcc
     5221 ggcacccgac caggtagacc gataccaccc cgggggcctc ggtcaccacg ttcgccacgc
     5281 gcagccgatg gcgcagcgag atggcgatcg gctgcccgac gcggaaggtc agcaccatgg
     5341 cagccaccgc gacgtagagg ctgatccagt agatccgcgc caccgggtcg tgcatgaagt
     5401 cgctgccgac gaccagctgg tgcgccgttg ccagcgcgat ggcgaggtag ccgtagaggt
     5461 ggattccgtg ccatgtctcg taggcgagct tgcgacgcgc gatgcgcacc gaggtgacgc
     5521 ccaccgcgat cagcatcgcc atgccggccg tcgccatgag cacgtacggg aaggtggtca
     5581 gcagcagcca cagctcaccg atcggcgagg tgtggccgat cgccgcgtac ccgatggtgg
     5641 tgaacgcacc gtggccgagc agcagccaca cggtcgcgaa gccgatccat cggtgccagg
     5701 cggccaggcg atgcatcccg aagagctggt cgagccaggg cgagcgggac atcagcacca
     5761 gctgcaccag cgcggtgtag gtgccgagca gcgcggtcag ctgccccgcc gcgatgaacg
     5821 gcccgcctgg cgtcgggatc tggtcgagcc caccgtggcg ccaccacatc gcggcgatga
     5881 acagcccgtt gccgaccagc agggccgcca cgtcgattgg cttcagccgc caggtgcgcg
     5941 gcatcggcac gcgtgcaagg gtggtcggga tggcagccat gcgtcgatgg tgccggtcgt
     6001 tcctgagagt gtcctgagag ccaatgattc ccgatgcggc ggatcagcga ccgggtcggg
     6061 ctatgggatg gtgtggtggc cgaggtgggc tgatcggcct gggagccgcg caggttgggg
     6121 tagacccaaa cgatccagac gtggagaatc gctgccatgg acacgcgcat gacgcatgcc
     6181 gcggtggagg aggccgcgac gccgctcacg gacgcaacgc tcggcgcgct gcgggaggca
     6241 ttccgtgatc attgacatga cggcaggccc gccaccggag acaccgccgg gcgccgcacc
     6301 gagcgggccg ccacccgcct tgatggccct cgtgcacaag gtgcagcgcg gcggcatgtt
     6361 cctgagcgtg atgatcgccg tgatcgtgtt tctgatggtg gtgaagccga acctcggctt
     6421 ctgacgggcc gctagtcgag cgactcggcg acccgggccg cctccgccag cgagggggcg
     6481 ccctcgacgc gataggtgac cccgccctcc tcccagatca gggtcgggct ggcaaggcgg
     6541 agggtcgagt cgacgatatg cccctgagca tcacggtaga agaagaagtg gtctccgccc
     6601 gccacccacc aggcgggttc gccgtgcacc cgggcaggca ttacacgcac gccgccctcg
     6661 atcagcttct cgaacacgtc cggcccgatg tcggctcgga actgggtcac gatcaggccg
     6721 attcccgagg cggggtccgt cgggaatccg gaccgttcgc cgtacaacag cgtcaccgct
     6781 cctccggcag gcgggtccgc caggtagaca cggtcgggct ggccgagctc gggtagccct
     6841 gggacacgca gggtgaaggt caccccgccc cgcgccgcat ccagggaggt cggcgctcca
     6901 agaccgggtc ccaccaccat gctcgggatc ggcgagaagc tcgcgggtcc gaataccagc
     6961 cgcaggccgc cgacgccgat cccgaatgcc accgcgatcc cggccagcag gaccgtcgct
     7021 gccatggcca gcgccaggcc gcggctgacc gggcgcccga gaccgatccg cgggggcgcc
     7081 tgagccaggc gctctccgac cagcgcgacg agcgggggcg tggcgggaaa ctcgacctca
     7141 gcggccagct cggccaggcg agcttccaac cgatcccggc gatcgtcaaa catcggatgc
     7201 ctccccgatc gcttcccgca ggcgggcggt ggcccgtgac agccgggact tgacggtgcc
     7261 gcgcgggcac gccaggacgg cggccatttc ctcctcgctg agatccagga accagcgcag
     7321 cgcgatcacc aggcgatcgt cgacgcgcag cccattgatc gcggccagga gctcgcgcct
     7381 tcgctcgtcg gcaaggagca gcacctcggg cgatggggcc gcatcgcccg agactgcatc
     7441 gtggaacgcc cgcagcgcca ggtcggcgcg gcgcgacgcg ctccgccggc ggttccgcgc
     7501 ggcgttggcc acgatccgga gcagccacgg gcgaaactca gcgccttcgc gaaagcgccc
     7561 cagcgcagcg tgcgcgcgga caaagccatc ctgggccgca tctcgcgcct catcggcatc
     7621 accgatcacc aggtacgcgg tccgaaaggc gatctcctgg tagtggcgca cgagctcctc
     7681 gaaggccgac gcatcaccgc gtcgagccga ttcgatccat tcgcgttcga gcagtggacg
     7741 gccctccacc tctgccaccc ctacaccgcc catcgtgcgc cggttccatc ctgctggaaa
     7801 aaggaagagg cccggcgatt gctcgccggg cctcttgtcg ctgagcggct tagcgccgcg
     7861 tgctgaagca gttgctgcag tagacgggct tggagccggt cggacggaac ggaacgcgcg
     7921 cctcgccgcc gcaaccatcg caggttgcgg tgaacatctc gcgctgaccg ccgccgtagc
     7981 tggagccacc gccgtagcct gagccactgc cgtaactgga gccgctgctg tagccgccgc
     8041 tctcgccgcg tgcggccttg cgggctgcgc gacaggaggc gcagcggcgc ggctcgctga
     8101 attccttctc cgcgtagaac tgctgatcag tcgccgcgaa gacgaattcc tggttgcaat
     8161 cgacgcagct caggatcttg tctgaatagg tggacggggt atacaaagag aaaactcctt
     8221 ctcgtgcggc ctcagcgatc ctgtctcgcc gtaaagccgt gaggaggagt gaacgtcgat
     8281 gccgcgggtc ttatgtcccc gccattcggg gccttgtcct cgcaccgtac cacatctcgc
     8341 cgaattagca aacaggaccc gcatcgctag agcccgtcgc ctgccttcgt gatgcgatgt
     8401 cgggtctgga catccacctt cgagtcacgt ccctcgtggg caggatgctc gaccagcgcg
     8461 atcagccggc tggacatgaa gcgtccggtg cggaccggcg tgccgccgcg ggtcacctcg
     8521 ctgacctcga ccactcctcg actggtggtc acctcgatcc ggcgaccggc gcgggtcgct
     8581 tcgatctcga aggtctgcgt gccctggccg gtgtcgacca cggcctccac tcgatcgccc
     8641 ttcatgaatt ctccctctgg tgagcacgcc cgtccgcgcg atgctacccg gatatgctgc
     8701 ccgaggccat aattcgttcc tcgcagggaa tggtcatgac cgatcgattc gtgtcgcgcg
     8761 gcttcatggg gcgtgcccgg gatcctggcg acaaggccag ccgcctgccg ccgggccagt
     8821 acctgaccga tggattcccc gtcctctctg ccggccccac gccgcaggtg ccgcttgaca
     8881 cctgggagtt cacgatcacc ggcctcctaa agacacccat ccgatggagc tggcaggact
     8941 tcatcgcgct cccatcgcgc gattggacgg tcgacatcag ctgcgtgacc aagtggacca
     9001 agctcgacac catctggcat ggggtgagcc tcgacgacat cctggccgcc gtcgagatcg
     9061 agccgcgggc tcgcttcgtg acggcacact cgtacggtgg ctacacgacc aacctgccgc
     9121 tgacggacct gctcaacaat caggccttcg tcgcctggca gtacggagga gcgccgctcg
     9181 agcccgcgca cggcggcccg gcgcgcctgg tggtgccgca cctctacttc tggaagagcg
     9241 caaagtgggt tcggggtctg cagctgcgcg aggacgacga gccaggcttc tgggaatcgc
     9301 tgggctacca caaccgcggc gacccctggc gcgaagagcg ctactcaggc gactgaagac
     9361 cctccagaga ccgatggccg agcccctcgc ctggcagatc tccacggtca gcgccattcg
     9421 atccgagacg ccggaggtca gctcgttcac gctgaagctg ccgaactggc gggctcatcg
     9481 ggccggacag cactacgacg tgcgcctgac tgcggccgat ggctaccagg cccagcgctc
     9541 gtactccatc gcctcgccac cgtcgaggtc aggcgaggtc gacctcacca tcgagcggat
     9601 cgcggacggg gaggtctcgc cgtactttca tgacatcgtc gaggtggggg accaggtcga
     9661 ggtgcgcggc cccatcggtg gctacttcgt gtgggagccc tcactcggtg ggccgctgct
     9721 cctcgtcgcc ggcgggtcgg gcgtggtgcc cctgatggcg atgctgcgcg agcgcgcatc
     9781 ggccgaccgg aagccaccgg cggtcctgct ctactcgtcg cgcaccgttg acgac
//
